TGS1 cDNA ORF clone, Corvus cornix cornix

Gene
Clones
gRNAs

Summary

Gene Symbol	TGS1
Entrez Gene ID	104690381
Full Name	trimethylguanosine synthase 1
Gene Type	protein-coding
Organism	Corvus cornix cornix

mRNA and Protein(s)

mRNA	Protein	Name
XM_010401163.3	XP_010399465.2	LOW QUALITY PROTEIN: trimethylguanosine synthase

Pathways from BioSystems
Gene Ontology

The following TGS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo99591	XM_010401163.3 Latest version!	Corvus cornix cornix trimethylguanosine synthase 1 (TGS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TGS1 ( XM_010401163.3 ) cDNA ORF clone, Corvus cornix cornix -> XP_010399465.2 Corvus cornix cornix trimethylguanosine synthase 1 (TGS1), mRNA.

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CloneID	OCo99591
Clone ID Related Accession (Same CDS sequence)	XM_010401163.3
Accession Version	XM_010401163.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-21
Organism	Corvus cornix cornix
Product	LOW QUALITY PROTEIN: trimethylguanosine synthase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018114003.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 22, 2017 this sequence version replaced XM_010401163.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Corvus cornix cornix Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGTGCAGG ACCCGGGCCC GAGGCTGGTG GCCGAGCTGC TGCTGCGGGC GGGGCCGGCC 
GGCGGCATCC TCTGCCTCTG CTCCCGCGCC TTCGTCGAAG ATCGAAAATT ATATAAACTG 
GGATTAAAAG GATTTTATGT CAAAGATGGC AATGACAGTA CAGGGGAAGA ACAAGCATCT 
GAGGAGGAGA ACCGTTATCC CAATGGGAGA TTAAAGGTGG ATACTAATCA TGCCCTGGGC 
GTGGAAGAAG TTGAACTAGA CTCAGAAGCT GAGCTTATGA AGAGCATGGG ATTGCCTCTT 
CAGTTTGGTG GGCAGTCAGC CCACAGGGAC TTCGTGGCAA CAGAAAATTA CAGAAAGAAA 
AATAACATGA AGATTATGAA AAAGAAAAAG AAGAAGAAAA AAGAGTTGCA GCAAAAACAT 
GAGGATAAAA TGGGGCAGGA ATGCCAGGAC AAAACTTGTG GTGGTTGTAT CCAGTCCGTT 
TCTGGTGAGC TGGCCCTAGC TACAGAGCAG TGTGAGATGA GCAGCAAACC TCAAGCTTTA 
GTTGAAGGGA ATTGTGAAAG TTCAGAAAGC CTTGCAAGTG AGACTTTACC CAGTGAACTT 
AAGGAAAAAT GGGAGAAGTA TTGGAGCGAG TACGGAGAGG GCCTTCTATG GCAAAGTTGG 
TTGAAAAAGC ACCAAGAGGT CTCATTGTCT GAGGGCATCA CAGCCTCTGA GCCTTGGAAT 
AGTCCAGATA CTAAGGAAGA ATGGGAGCAG CATTATAGTG AACTGTACTG GTATTATTGG 
GAACAGTTTC AGTACTGGAC AAGTCAAGGA TGGACTACTG AGAGCTCACA TGGTGACAAG 
GTGGAAGTTA ATGGGGTTAC ACAGGAGACT GGTCCTTTGA GAGAAATGGA CTTGGTTAGC 
CCAGGACCTG AGCACAGTGA AGTGCTGAGC TTGAAGCTGT CTCCCTCCAA CACCAGAAGT 
GAGGAAAGAC TCCCTTCAAG TGCTGAGCCC CACAGTGAAA TAATCTCTGG GATTTGTAAT 
TTAAATCTGA ATTTGGAGGA AGTGGAACAG AGCAGTGCTG CTCTAACTGT AGCCCACAGT 
GGTCCTGAGG AACAGAGTTC GTCTGACAGT GGAAGCCAGA AGGAGCCCTG TGATGGAGGA 
AGCAGGAAAA GGAGAGCATC TTGTGAAAAT AAAAGTGTTA ACCAGTCAGG TGTGCAGGAG 
CCATGCAGCT CTAATTCAAA TGAAAAAGAG CAGTTGCTGC TTCATGACCA AGATGAAGAT 
GAGGATGAAG AGCCTCCTGA ATACAGACAT GTCAAAGTCA AGAGAAGCCA TGAGCTGGAT 
GTGGATGAGA ACCCCATGGA AGATCCTGAG GAAACCTGCT CTGTGTTGGG TTTCAAGTGT 
GGCACAGGAC AAAAATATGG TGGAATTCCA GATTTCACCC ACCAAAGTGT GCAGTACTTG 
GAGAAAAAAG CAAAACTCAA GTCCAAATTC TTGGATATGC GTAAACCAAG GAAGAGCAAA 
AACACACACA TCTTCTTTAC AGAGGAATCT GAAATGATAT GCAAAAAAAG CAAAACTTTG 
AAGAAGGTGG AAATGTTCCT GAAGCAGGTT AGTAAACCTG AGGAGGAAGA CACGTCCCAG 
CCAGCCACCC CTCAGGGTAA AGCAGAGGCA TTGTCTGCGA GCAGCAGCGA CTCAGAGGGT 
CAGGAAGGCG TTGCTGCCAC ACAGAGTGTG ACGCTGGACA CTGAAATCCA AGAGCCACCC 
TCTTCCAGTG TGGCCTGCAC AGAACCTGGA GGGAGTAACC TTGAGGGGGA GGTGCAGGAC 
ACTGCAGCGA GTGGCCCTGA TGAGCAGGGG GCAGGGAGGC CAGAGCACAG CGGTGGGCGG 
CAGCTTGTCC CTCTGGATAT TCCCGATTAT CTCCAGCCAG ACACGGAGGA TGTCAGCCAA 
GCTGTAGATG AAAAAATTAC TGTGAAGAAC AAGGAGAAGA AAAGAAGAAG AAGGAATAAA 
AATGCACTGA GAACTATACC TCCTGAGATT GCTGCTGATC CAGAGCTGGT CAAGTACTGG 
GCACAGCGCT ACCGGCTCTT CTCTCGGTTT GATGAAGGAA TTAAACTAGA CAGAGAGGGT 
TGGTTTTCTG TTACTCCTGA GAAAATAGCT GAGCACATTG CTGTCCGCGT TAGTCAGTCA 
TTCAACTGCA ACATCATAGT GGATGCGTTC TGTGGGGTTG GAGGGAACGC TATTCAGTTT 
GCACTGACCT CAAAAAGAGT GATCGCCGTC GACATTGACC CCGAGAAGCT GCGGCTGGCG 
CGGCACAACG CGGAGGTGTA CGGCGTGGCC GAGCACATCG ACTTCCTCTG CGGGGACTTC 
ATGGCGCTGG CGGCCGGCCT GCGCGCGGAC GCGGTGTTCC TCAGCCCGCC CTGGGGCGGG 
CCCGACTACG CCACTGCCGA AATCTTCGAC ATCCACACCA TGATTTGCCC AGACGGCTTT 
GAAATTTTCA GGCTCTCCAA GAAGATCACC AACAATATTG TGTATTTTCT GCCTCGGAAT 
GCTGATATTA ACCAGGTGGC TTCCTTAGCA GGACCAGGAG GGAAAGTTGA AATAGAACAA 
AATTTTCTCA ATAACAAACT GAAGACAATA ACAGCTTATT TTGGTGATCT AATAAGGCAT 
GACATCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010399465.2
CDS	44..2695
Translation	MVQDPGPRLVAELLLRAGPAGGILCLCSRAFVEDRKLYKLGLKGFYVKDGNDSTGEEQASEEENRYPNGRLKVDTNHALGVEEVELDSEAELMKSMGLPLQFGGQSAHRDFVATENYRKKNNMKIMKKKKKKKKELQQKHEDKMGQECQDKTCGGCIQSVSGELALATEQCEMSSKPQALVEGNCESSESLASETLPSELKEKWEKYWSEYGEGLLWQSWLKKHQEVSLSEGITASEPWNSPDTKEEWEQHYSELYWYYWEQFQYWTSQGWTTESSHGDKVEVNGVTQETGPLREMDLVSPGPEHSEVLSLKLSPSNTRSEERLPSSAEPHSEIISGICNLNLNLEEVEQSSAALTVAHSGPEEQSSSDSGSQKEPCDGGSRKRRASCENKSVNQSGVQEPCSSNSNEKEQLLLHDQDEDEDEEPPEYRHVKVKRSHELDVDENPMEDPEETCSVLGFKCGTGQKYGGIPDFTHQSVQYLEKKAKLKSKFLDMRKPRKSKNTHIFFTEESEMICKKSKTLKKVEMFLKQVSKPEEEDTSQPATPQGKAEALSASSSDSEGQEGVAATQSVTLDTEIQEPPSSSVACTEPGGSNLEGEVQDTAASGPDEQGAGRPEHSGGRQLVPLDIPDYLQPDTEDVSQAVDEKITVKNKEKKRRRRNKNALRTIPPEIAADPELVKYWAQRYRLFSRFDEGIKLDREGWFSVTPEKIAEHIAVRVSQSFNCNIIVDAFCGVGGNAIQFALTSKRVIAVDIDPEKLRLARHNAEVYGVAEHIDFLCGDFMALAAGLRADAVFLSPPWGGPDYATAEIFDIHTMICPDGFEIFRLSKKITNNIVYFLPRNADINQVASLAGPGGKVEIEQNFLNNKLKTITAYFGDLIRHDIS

Target ORF information:

RefSeq Version	XM_010401163.3
Organism	Corvus cornix cornix
Definition	Corvus cornix cornix trimethylguanosine synthase 1 (TGS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010401163.3

ORF Insert Sequence:

ATGGTGCAGG ACCCGGGCCC GAGGCTGGTG GCCGAGCTGC TGCTGCGGGC GGGGCCGGCC 
GGCGGCATCC TCTGCCTCTG CTCCCGCGCC TTCGTCGAAG ATCGAAAATT ATATAAACTG 
GGATTAAAAG GATTTTATGT CAAAGATGGC AATGACAGTA CAGGGGAAGA ACAAGCATCT 
GAGGAGGAGA ACCGTTATCC CAATGGGAGA TTAAAGGTGG ATACTAATCA TGCCCTGGGC 
GTGGAAGAAG TTGAACTAGA CTCAGAAGCT GAGCTTATGA AGAGCATGGG ATTGCCTCTT 
CAGTTTGGTG GGCAGTCAGC CCACAGGGAC TTCGTGGCAA CAGAAAATTA CAGAAAGAAA 
AATAACATGA AGATTATGAA AAAGAAAAAG AAGAAGAAAA AAGAGTTGCA GCAAAAACAT 
GAGGATAAAA TGGGGCAGGA ATGCCAGGAC AAAACTTGTG GTGGTTGTAT CCAGTCCGTT 
TCTGGTGAGC TGGCCCTAGC TACAGAGCAG TGTGAGATGA GCAGCAAACC TCAAGCTTTA 
GTTGAAGGGA ATTGTGAAAG TTCAGAAAGC CTTGCAAGTG AGACTTTACC CAGTGAACTT 
AAGGAAAAAT GGGAGAAGTA TTGGAGCGAG TACGGAGAGG GCCTTCTATG GCAAAGTTGG 
TTGAAAAAGC ACCAAGAGGT CTCATTGTCT GAGGGCATCA CAGCCTCTGA GCCTTGGAAT 
AGTCCAGATA CTAAGGAAGA ATGGGAGCAG CATTATAGTG AACTGTACTG GTATTATTGG 
GAACAGTTTC AGTACTGGAC AAGTCAAGGA TGGACTACTG AGAGCTCACA TGGTGACAAG 
GTGGAAGTTA ATGGGGTTAC ACAGGAGACT GGTCCTTTGA GAGAAATGGA CTTGGTTAGC 
CCAGGACCTG AGCACAGTGA AGTGCTGAGC TTGAAGCTGT CTCCCTCCAA CACCAGAAGT 
GAGGAAAGAC TCCCTTCAAG TGCTGAGCCC CACAGTGAAA TAATCTCTGG GATTTGTAAT 
TTAAATCTGA ATTTGGAGGA AGTGGAACAG AGCAGTGCTG CTCTAACTGT AGCCCACAGT 
GGTCCTGAGG AACAGAGTTC GTCTGACAGT GGAAGCCAGA AGGAGCCCTG TGATGGAGGA 
AGCAGGAAAA GGAGAGCATC TTGTGAAAAT AAAAGTGTTA ACCAGTCAGG TGTGCAGGAG 
CCATGCAGCT CTAATTCAAA TGAAAAAGAG CAGTTGCTGC TTCATGACCA AGATGAAGAT 
GAGGATGAAG AGCCTCCTGA ATACAGACAT GTCAAAGTCA AGAGAAGCCA TGAGCTGGAT 
GTGGATGAGA ACCCCATGGA AGATCCTGAG GAAACCTGCT CTGTGTTGGG TTTCAAGTGT 
GGCACAGGAC AAAAATATGG TGGAATTCCA GATTTCACCC ACCAAAGTGT GCAGTACTTG 
GAGAAAAAAG CAAAACTCAA GTCCAAATTC TTGGATATGC GTAAACCAAG GAAGAGCAAA 
AACACACACA TCTTCTTTAC AGAGGAATCT GAAATGATAT GCAAAAAAAG CAAAACTTTG 
AAGAAGGTGG AAATGTTCCT GAAGCAGGTT AGTAAACCTG AGGAGGAAGA CACGTCCCAG 
CCAGCCACCC CTCAGGGTAA AGCAGAGGCA TTGTCTGCGA GCAGCAGCGA CTCAGAGGGT 
CAGGAAGGCG TTGCTGCCAC ACAGAGTGTG ACGCTGGACA CTGAAATCCA AGAGCCACCC 
TCTTCCAGTG TGGCCTGCAC AGAACCTGGA GGGAGTAACC TTGAGGGGGA GGTGCAGGAC 
ACTGCAGCGA GTGGCCCTGA TGAGCAGGGG GCAGGGAGGC CAGAGCACAG CGGTGGGCGG 
CAGCTTGTCC CTCTGGATAT TCCCGATTAT CTCCAGCCAG ACACGGAGGA TGTCAGCCAA 
GCTGTAGATG AAAAAATTAC TGTGAAGAAC AAGGAGAAGA AAAGAAGAAG AAGGAATAAA 
AATGCACTGA GAACTATACC TCCTGAGATT GCTGCTGATC CAGAGCTGGT CAAGTACTGG 
GCACAGCGCT ACCGGCTCTT CTCTCGGTTT GATGAAGGAA TTAAACTAGA CAGAGAGGGT 
TGGTTTTCTG TTACTCCTGA GAAAATAGCT GAGCACATTG CTGTCCGCGT TAGTCAGTCA 
TTCAACTGCA ACATCATAGT GGATGCGTTC TGTGGGGTTG GAGGGAACGC TATTCAGTTT 
GCACTGACCT CAAAAAGAGT GATCGCCGTC GACATTGACC CCGAGAAGCT GCGGCTGGCG 
CGGCACAACG CGGAGGTGTA CGGCGTGGCC GAGCACATCG ACTTCCTCTG CGGGGACTTC 
ATGGCGCTGG CGGCCGGCCT GCGCGCGGAC GCGGTGTTCC TCAGCCCGCC CTGGGGCGGG 
CCCGACTACG CCACTGCCGA AATCTTCGAC ATCCACACCA TGATTTGCCC AGACGGCTTT 
GAAATTTTCA GGCTCTCCAA GAAGATCACC AACAATATTG TGTATTTTCT GCCTCGGAAT 
GCTGATATTA ACCAGGTGGC TTCCTTAGCA GGACCAGGAG GGAAAGTTGA AATAGAACAA 
AATTTTCTCA ATAACAAACT GAAGACAATA ACAGCTTATT TTGGTGATCT AATAAGGCAT 
GACATCTCCT GA