UBE3B cDNA ORF clone, Corvus cornix cornix

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE3B
Entrez Gene ID	104688656
Full Name	ubiquitin protein ligase E3B
Gene Type	protein-coding
Organism	Corvus cornix cornix

mRNA and Protein(s)

mRNA	Protein	Name
XM_010398282.3	XP_010396584.1	ubiquitin-protein ligase E3B isoform X1
XM_019285154.2	XP_019140699.1	ubiquitin-protein ligase E3B isoform X2

Pathways from BioSystems
Gene Ontology

The following UBE3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo103342	XM_019285154.2 Latest version!	Corvus cornix cornix ubiquitin protein ligase E3B (UBE3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCo103341	XM_010398282.3 Latest version!	Corvus cornix cornix ubiquitin protein ligase E3B (UBE3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE3B ( XM_019285154.2 ) cDNA ORF clone, Corvus cornix cornix -> XP_019140699.1 Corvus cornix cornix ubiquitin protein ligase E3B (UBE3B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCo103342
Clone ID Related Accession (Same CDS sequence)	XM_019285154.2
Accession Version	XM_019285154.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-21
Organism	Corvus cornix cornix
Product	ubiquitin-protein ligase E3B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018114020.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Mar 22, 2017 this sequence version replaced XM_019285154.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Corvus cornix cornix Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGTG CCAGCCAGTC GTCCAGGGCT CAGTTCCTGG ACAAGGCCCG GCAGGCGCGG 
GAGGAGCGGC GGGAGCTCAA GGAGAGGGAG AGAGCTGCAG TCCAGCTCCA GGCTCTGGTC 
AGGAGGTTCC TGTGTCGATG CCGCCTGCAG AGGGAGATCA GGAGAGAGGT GGAGGATTTC 
TTTGGGACAA ATGAATGTGG CTCAAGTAAG AGAAGTGCCC TGTCTGTGTT CAGAATTGCC 
AGGAAGCTGC TGTTTGTGTT CAATCCCAAG GAGGACAAGG AGAGGTTTGA AAAGCTGTGC 
CGTTGTATCC TGAACAGCAT GGATGTGGAG AATGAGCCCA AGGTGTGGTA CGTGTCACTG 
GCACTCTCCA AGGACCTCAC ACTCCTCTGG ATCAAGCAGA TCAAAGACAT CCTGTGGTTT 
TGCTGTGAGT TCCTCAAGCA GCTCAAGCCT GATATCCTGC AGGACTCCAG GCTGGTGAAC 
TTGCACCTCA CAATGCTGGT CACCTTCACA GACACTTCCA CCTGGAAAAT CCTCCGGGGA 
AAAGGTGAAA CCCTGAGACC TGCCATGAAC CACATCTGTG CCAACATCAT GGGCCACCTG 
AACCAGAAGG GCTTCTACTC CGTGCTGCAG ATTTTGCTAA CTAATGGCTT GGCAAGATCC 
AGACCTTCCC TGTCCAAAGG CTCCTTAACT GCCATCTTCT CGCTTGCCTT GCGCCCTGTG 
GTTGCTGCAC AGTTCTCAGA CAATCTGCTG AGGTCCTTCC TGATCCATGT CATGTCTGTG 
CCTGCTATAA TGACTCACCT TGCTACTCTC ACCCCTGAGC GCCTGGCAGT GATCGAATCC 
CACGAGCTCT TCCGGAAGTT CATCCTGTTT CTGAGCCGAG AATCCCAGTG CCGGGATGTC 
TGCGTGTGCC TGGAGGGCAG TCACACTCTC TGCTTGCTGG GCAATCTGGT GTTCCTGGGC 
TTCCTGAATG ACCAGGTGCT GGAGGAGGAG ACAGCTCATT TTGTGGGGGT GCTCATCCAC 
ATGCTCTCCT ACTGCCAGAA GTACGTGTCC CAGAAGAAAT CCAACCTCAC CCACTGGCAC 
CCTGTGCTGG GATGGTTCTC ACAGACTGTG GATTATGGGC TGAACGAGTC CATGCCCCTG 
CTGACCAAGC AGCTGCAGCA CCTCTGGGGA GTCCACATGA TCCGTATCCT CTTCAGTGAT 
GTGCTCAGCA AGAAACTGCT GGAAAATCAG GAGATAGCTC AGCTGCCAGC ACAGCCAGTC 
TCCCCCCAGA ACAGCCTTCC TATGAAAAAC CTGTTCAAGA GAGCTTTCCA GAAGTCGGCC 
TCAGTCCGGA ACATCCTGAA GCCGGTGGGA GGGAAGCGCG TGGACTCTGC CGAGGTGCAG 
AAGGTTTGCA GCATCTGTGT CCTGTACCAA ACCACCCTGA CCACCCTCAC CCAGATCCGC 
CTGCAGATCC TCACAGGTCT CACTTACCTG GATGATCTGC TGCCCAAATT ATGGGCTTTC 
ATCTGTGAGC TGGGACCACA GGGGGGGTTA AAACTCTTCT TGGAGTGTTT GAACAACGAC 
ACAGAGGAAT CCAAGAGGCT GCTGGCCATG TTGATGCTCT TCTGTGACTG CTCCCGTCAC 
CTCATCACGA TTCTGGATGA CATCGAGGTC TACGAAGAGC AGATTTCATT CAAACTGGAA 
GAGCTGGTGA CCATCTCCTC TTTCCTGAAT TCCTTTGTGT TTAAGATGAT CTGGGATGGA 
ATTGTAGAGA ATGCCCGAGG GGAGACCCTG GAGCTGTTCC ACTCTGTCCA TGGCTGGCTC 
ATGGTGCTGT ACGAGCGGGA TTGTCGCCGG CGCTTCGCTC CCGAGGATCA CTGGCTGCGC 
AAGGACCTCA AACCCAGCGT GCTCTTCCAG GAGTTGGACA AGGACAAGAA ACGAGCCCAG 
CTGCTGCTGC AGTACATCCC ACACGTCATT CCCCATAAGA ACAGGGTGCT GCTCTTCCGG 
AACATGGTCA CAAAGGAGAA GGAGAAGCTG GGGCTGGTGG AGACGAGCTC GGCTTCCCCT 
CACGTCACAC ACATCACCAT CCGCCGCTCC CGCATGCTGG AGGATGGATA CGAGCAGCTC 
CGGCAGCTTT CCCAGAACGC CATGAAGGGA GTGATCAGGG TGAAGTTTGT CAATGATCTG 
GGGGTTGATG AGGCTGGTAT TGATCAAGAT GGAGTCTTCA AAGAGTTCCT GGAAGAGATC 
ATTAAAAAGG TGTTTGACCC TGCACTGAAT CTGTTCAAGA CCACCAGTGG TGATGAGAGG 
CTGTATCCAT CCCCAACATC CTACATCCAT GAGAACTACC TGCAGCTCTT TGAGTTCGTG 
GGGAAGATGC TTGGGAAGGC TGTGTATGAG GGAATAGTTG TGGATGTACC ATTTGCTTCC 
TTCTTTCTGA GTCAGCTCCT CGGGCACCAC CACAGTGTCT TCTACAGCTC CGTGGATGAA 
CTGCCCTCCT TGGACTCGGA GTTCTATAAA AATCTCACTT CAATTAAGCG TTATGATGGT 
GATATCAGTG ACCTGGGCTT GACACTGTCC TATGATGAAG ATGTGATGGG TCAGCTGGTT 
TGCCATGAAC TTGTTCCTGG AGGGAAGACC ATTCCCGTTA CCAATGAAAA TAACACTTCT 
GAGGTCAAAG GCACCCAGTC AGGGAGATTC TGCAGGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019140699.1
CDS	154..2835
Translation	MFSASQSSRAQFLDKARQAREERRELKERERAAVQLQALVRRFLCRCRLQREIRREVEDFFGTNECGSSKRSALSVFRIARKLLFVFNPKEDKERFEKLCRCILNSMDVENEPKVWYVSLALSKDLTLLWIKQIKDILWFCCEFLKQLKPDILQDSRLVNLHLTMLVTFTDTSTWKILRGKGETLRPAMNHICANIMGHLNQKGFYSVLQILLTNGLARSRPSLSKGSLTAIFSLALRPVVAAQFSDNLLRSFLIHVMSVPAIMTHLATLTPERLAVIESHELFRKFILFLSRESQCRDVCVCLEGSHTLCLLGNLVFLGFLNDQVLEEETAHFVGVLIHMLSYCQKYVSQKKSNLTHWHPVLGWFSQTVDYGLNESMPLLTKQLQHLWGVHMIRILFSDVLSKKLLENQEIAQLPAQPVSPQNSLPMKNLFKRAFQKSASVRNILKPVGGKRVDSAEVQKVCSICVLYQTTLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPQGGLKLFLECLNNDTEESKRLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENARGETLELFHSVHGWLMVLYERDCRRRFAPEDHWLRKDLKPSVLFQELDKDKKRAQLLLQYIPHVIPHKNRVLLFRNMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQNAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKKVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDISDLGLTLSYDEDVMGQLVCHELVPGGKTIPVTNENNTSEVKGTQSGRFCRI

Target ORF information:

RefSeq Version	XM_019285154.2
Organism	Corvus cornix cornix
Definition	Corvus cornix cornix ubiquitin protein ligase E3B (UBE3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019285154.2

ORF Insert Sequence:

ATGTTCAGTG CCAGCCAGTC GTCCAGGGCT CAGTTCCTGG ACAAGGCCCG GCAGGCGCGG 
GAGGAGCGGC GGGAGCTCAA GGAGAGGGAG AGAGCTGCAG TCCAGCTCCA GGCTCTGGTC 
AGGAGGTTCC TGTGTCGATG CCGCCTGCAG AGGGAGATCA GGAGAGAGGT GGAGGATTTC 
TTTGGGACAA ATGAATGTGG CTCAAGTAAG AGAAGTGCCC TGTCTGTGTT CAGAATTGCC 
AGGAAGCTGC TGTTTGTGTT CAATCCCAAG GAGGACAAGG AGAGGTTTGA AAAGCTGTGC 
CGTTGTATCC TGAACAGCAT GGATGTGGAG AATGAGCCCA AGGTGTGGTA CGTGTCACTG 
GCACTCTCCA AGGACCTCAC ACTCCTCTGG ATCAAGCAGA TCAAAGACAT CCTGTGGTTT 
TGCTGTGAGT TCCTCAAGCA GCTCAAGCCT GATATCCTGC AGGACTCCAG GCTGGTGAAC 
TTGCACCTCA CAATGCTGGT CACCTTCACA GACACTTCCA CCTGGAAAAT CCTCCGGGGA 
AAAGGTGAAA CCCTGAGACC TGCCATGAAC CACATCTGTG CCAACATCAT GGGCCACCTG 
AACCAGAAGG GCTTCTACTC CGTGCTGCAG ATTTTGCTAA CTAATGGCTT GGCAAGATCC 
AGACCTTCCC TGTCCAAAGG CTCCTTAACT GCCATCTTCT CGCTTGCCTT GCGCCCTGTG 
GTTGCTGCAC AGTTCTCAGA CAATCTGCTG AGGTCCTTCC TGATCCATGT CATGTCTGTG 
CCTGCTATAA TGACTCACCT TGCTACTCTC ACCCCTGAGC GCCTGGCAGT GATCGAATCC 
CACGAGCTCT TCCGGAAGTT CATCCTGTTT CTGAGCCGAG AATCCCAGTG CCGGGATGTC 
TGCGTGTGCC TGGAGGGCAG TCACACTCTC TGCTTGCTGG GCAATCTGGT GTTCCTGGGC 
TTCCTGAATG ACCAGGTGCT GGAGGAGGAG ACAGCTCATT TTGTGGGGGT GCTCATCCAC 
ATGCTCTCCT ACTGCCAGAA GTACGTGTCC CAGAAGAAAT CCAACCTCAC CCACTGGCAC 
CCTGTGCTGG GATGGTTCTC ACAGACTGTG GATTATGGGC TGAACGAGTC CATGCCCCTG 
CTGACCAAGC AGCTGCAGCA CCTCTGGGGA GTCCACATGA TCCGTATCCT CTTCAGTGAT 
GTGCTCAGCA AGAAACTGCT GGAAAATCAG GAGATAGCTC AGCTGCCAGC ACAGCCAGTC 
TCCCCCCAGA ACAGCCTTCC TATGAAAAAC CTGTTCAAGA GAGCTTTCCA GAAGTCGGCC 
TCAGTCCGGA ACATCCTGAA GCCGGTGGGA GGGAAGCGCG TGGACTCTGC CGAGGTGCAG 
AAGGTTTGCA GCATCTGTGT CCTGTACCAA ACCACCCTGA CCACCCTCAC CCAGATCCGC 
CTGCAGATCC TCACAGGTCT CACTTACCTG GATGATCTGC TGCCCAAATT ATGGGCTTTC 
ATCTGTGAGC TGGGACCACA GGGGGGGTTA AAACTCTTCT TGGAGTGTTT GAACAACGAC 
ACAGAGGAAT CCAAGAGGCT GCTGGCCATG TTGATGCTCT TCTGTGACTG CTCCCGTCAC 
CTCATCACGA TTCTGGATGA CATCGAGGTC TACGAAGAGC AGATTTCATT CAAACTGGAA 
GAGCTGGTGA CCATCTCCTC TTTCCTGAAT TCCTTTGTGT TTAAGATGAT CTGGGATGGA 
ATTGTAGAGA ATGCCCGAGG GGAGACCCTG GAGCTGTTCC ACTCTGTCCA TGGCTGGCTC 
ATGGTGCTGT ACGAGCGGGA TTGTCGCCGG CGCTTCGCTC CCGAGGATCA CTGGCTGCGC 
AAGGACCTCA AACCCAGCGT GCTCTTCCAG GAGTTGGACA AGGACAAGAA ACGAGCCCAG 
CTGCTGCTGC AGTACATCCC ACACGTCATT CCCCATAAGA ACAGGGTGCT GCTCTTCCGG 
AACATGGTCA CAAAGGAGAA GGAGAAGCTG GGGCTGGTGG AGACGAGCTC GGCTTCCCCT 
CACGTCACAC ACATCACCAT CCGCCGCTCC CGCATGCTGG AGGATGGATA CGAGCAGCTC 
CGGCAGCTTT CCCAGAACGC CATGAAGGGA GTGATCAGGG TGAAGTTTGT CAATGATCTG 
GGGGTTGATG AGGCTGGTAT TGATCAAGAT GGAGTCTTCA AAGAGTTCCT GGAAGAGATC 
ATTAAAAAGG TGTTTGACCC TGCACTGAAT CTGTTCAAGA CCACCAGTGG TGATGAGAGG 
CTGTATCCAT CCCCAACATC CTACATCCAT GAGAACTACC TGCAGCTCTT TGAGTTCGTG 
GGGAAGATGC TTGGGAAGGC TGTGTATGAG GGAATAGTTG TGGATGTACC ATTTGCTTCC 
TTCTTTCTGA GTCAGCTCCT CGGGCACCAC CACAGTGTCT TCTACAGCTC CGTGGATGAA 
CTGCCCTCCT TGGACTCGGA GTTCTATAAA AATCTCACTT CAATTAAGCG TTATGATGGT 
GATATCAGTG ACCTGGGCTT GACACTGTCC TATGATGAAG ATGTGATGGG TCAGCTGGTT 
TGCCATGAAC TTGTTCCTGG AGGGAAGACC ATTCCCGTTA CCAATGAAAA TAACACTTCT 
GAGGTCAAAG GCACCCAGTC AGGGAGATTC TGCAGGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( XM_010398282.3 ) cDNA ORF clone, Corvus cornix cornix -> XP_010396584.1 Corvus cornix cornix ubiquitin protein ligase E3B (UBE3B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCo103341
Clone ID Related Accession (Same CDS sequence)	XM_010398282.3
Accession Version	XM_010398282.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-21
Organism	Corvus cornix cornix
Product	ubiquitin-protein ligase E3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018114020.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 22, 2017 this sequence version replaced XM_010398282.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Corvus cornix cornix Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGTG CCAGCCAGTC GTCCAGGGCT CAGTTCCTGG ACAAGGCCCG GCAGGCGCGG 
GAGGAGCGGC GGGAGCTCAA GGAGAGGGAG AGAGCTGCAG TCCAGCTCCA GGCTCTGGTC 
AGGAGGTTCC TGTGTCGATG CCGCCTGCAG AGGGAGATCA GGAGAGAGGT GGAGGATTTC 
TTTGGGACAA ATGAATGTGG CTCAAGTAAG AGAAGTGCCC TGTCTGTGTT CAGAATTGCC 
AGGAAGCTGC TGTTTGTGTT CAATCCCAAG GAGGACAAGG AGAGGTTTGA AAAGCTGTGC 
CGTTGTATCC TGAACAGCAT GGATGTGGAG AATGAGCCCA AGGTGTGGTA CGTGTCACTG 
GCACTCTCCA AGGACCTCAC ACTCCTCTGG ATCAAGCAGA TCAAAGACAT CCTGTGGTTT 
TGCTGTGAGT TCCTCAAGCA GCTCAAGCCT GATATCCTGC AGGACTCCAG GCTGGTGAAC 
TTGCACCTCA CAATGCTGGT CACCTTCACA GACACTTCCA CCTGGAAAAT CCTCCGGGGA 
AAAGGTGAAA CCCTGAGACC TGCCATGAAC CACATCTGTG CCAACATCAT GGGCCACCTG 
AACCAGAAGG GCTTCTACTC CGTGCTGCAG ATTTTGCTAA CTAATGGCTT GGCAAGATCC 
AGACCTTCCC TGTCCAAAGG CTCCTTAACT GCCATCTTCT CGCTTGCCTT GCGCCCTGTG 
GTTGCTGCAC AGTTCTCAGA CAATCTGCTG AGGTCCTTCC TGATCCATGT CATGTCTGTG 
CCTGCTATAA TGACTCACCT TGCTACTCTC ACCCCTGAGC GCCTGGCAGT GATCGAATCC 
CACGAGCTCT TCCGGAAGTT CATCCTGTTT CTGAGCCGAG AATCCCAGTG CCGGGATGTC 
TGCGTGTGCC TGGAGGGCAG TCACACTCTC TGCTTGCTGG GCAATCTGGT GTTCCTGGGC 
TTCCTGAATG ACCAGGTGCT GGAGGAGGAG ACAGCTCATT TTGTGGGGGT GCTCATCCAC 
ATGCTCTCCT ACTGCCAGAA GTACGTGTCC CAGAAGAAAT CCAACCTCAC CCACTGGCAC 
CCTGTGCTGG GATGGTTCTC ACAGACTGTG GATTATGGGC TGAACGAGTC CATGCCCCTG 
CTGACCAAGC AGCTGCAGCA CCTCTGGGGA GTCCACATGA TCCGTATCCT CTTCAGTGAT 
GTGCTCAGCA AGAAACTGCT GGAAAATCAG GAGATAGCTC AGCTGCCAGC ACAGCCAGTC 
TCCCCCCAGA ACAGCCTTCC TATGAAAAAC CTGTTCAAGA GAGCTTTCCA GAAGTCGGCC 
TCAGTCCGGA ACATCCTGAA GCCGGTGGGA GGGAAGCGCG TGGACTCTGC CGAGGTGCAG 
AAGGTTTGCA GCATCTGTGT CCTGTACCAA ACCACCCTGA CCACCCTCAC CCAGATCCGC 
CTGCAGATCC TCACAGGTCT CACTTACCTG GATGATCTGC TGCCCAAATT ATGGGCTTTC 
ATCTGTGAGC TGGGACCACA GGGGGGGTTA AAACTCTTCT TGGAGTGTTT GAACAACGAC 
ACAGAGGAAT CCAAGAGGCT GCTGGCCATG TTGATGCTCT TCTGTGACTG CTCCCGTCAC 
CTCATCACGA TTCTGGATGA CATCGAGGTC TACGAAGAGC AGATTTCATT CAAACTGGAA 
GAGCTGGTGA CCATCTCCTC TTTCCTGAAT TCCTTTGTGT TTAAGATGAT CTGGGATGGA 
ATTGTAGAGA ATGCCCGAGG GGAGACCCTG GAGCTGTTCC ACTCTGTCCA TGGCTGGCTC 
ATGGTGCTGT ACGAGCGGGA TTGTCGCCGG CGCTTCGCTC CCGAGGATCA CTGGCTGCGC 
AAGGACCTCA AACCCAGCGT GCTCTTCCAG GAGTTGGACA AGGACAAGAA ACGAGCCCAG 
CTGCTGCTGC AGTACATCCC ACACGTCATT CCCCATAAGA ACAGGGTGCT GCTCTTCCGG 
AACATGGTCA CAAAGGAGAA GGAGAAGCTG GGGCTGGTGG AGACGAGCTC GGCTTCCCCT 
CACGTCACAC ACATCACCAT CCGCCGCTCC CGCATGCTGG AGGATGGATA CGAGCAGCTC 
CGGCAGCTTT CCCAGAACGC CATGAAGGGA GTGATCAGGG TGAAGTTTGT CAATGATCTG 
GGGGTTGATG AGGCTGGTAT TGATCAAGAT GGAGTCTTCA AAGAGTTCCT GGAAGAGATC 
ATTAAAAAGG TGTTTGACCC TGCACTGAAT CTGTTCAAGA CCACCAGTGG TGATGAGAGG 
CTGTATCCAT CCCCAACATC CTACATCCAT GAGAACTACC TGCAGCTCTT TGAGTTCGTG 
GGGAAGATGC TTGGGAAGGC TGTGTATGAG GGAATAGTTG TGGATGTACC ATTTGCTTCC 
TTCTTTCTGA GTCAGCTCCT CGGGCACCAC CACAGTGTCT TCTACAGCTC CGTGGATGAA 
CTGCCCTCCT TGGACTCGGA GTTCTATAAA AATCTCACTT CAATTAAGCG TTATGATGGT 
GATATCAGTG ACCTGGGCTT GACACTGTCC TATGATGAAG ATGTGATGGG TCAGCTGGTT 
TGCCATGAAC TTGTTCCTGG AGGGAAGACC ATTCCCGTTA CCAATGAAAA TAAGATCAGC 
TACATCCACC TGATGGCCCA TTTCCGGATG CACACCCAGA TCAAGAGCCA GACTGCAGCT 
CTCATCAGCG GCTTCAGGTC CATCATCAAG CCCGAGTGGA TCCGGATGTT TTCCGCGCCG 
GAGCTGCAGC GCCTCATCTC CGGGGACAAC GCCGAGATCG ACCTCGAGGA CCTGAAGAAG 
CACACGGTGT ACTACGGGGG CTTCCATGGC AGTCACCGCG TCATCATCTG GCTCTGGGAC 
ATCCTGGCCA ACGACTTCAG CCCTGAGGAG AGAGCCATGT TCCTCAAGTT TGTCACCAGC 
TGCTCCAGGC CTCCACTTCT GGGCTTTGCC TACCTCAAGC CTCCTTTCTC CATCCGCTGT 
GTGGAAGTGT CTGATGATCA GGACACGGGG GACACCCTGG GCAGCGTTCT GCGCGGCTTC 
TTCACCATCC GCAAGAAGGA GCCGGGCGGG CGCCTCCCGA CCTCCTCCAC ATGTTTCAAC 
CTCCTCAAGC TCCCCAACTA CAGCAAGAAG AGCATCCTGC GGGAGAAGCT GCGCTACGCC 
ATCAGCATGA ACACCGGCTT CGAGCTGTCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010396584.1
CDS	151..3363
Translation	MFSASQSSRAQFLDKARQAREERRELKERERAAVQLQALVRRFLCRCRLQREIRREVEDFFGTNECGSSKRSALSVFRIARKLLFVFNPKEDKERFEKLCRCILNSMDVENEPKVWYVSLALSKDLTLLWIKQIKDILWFCCEFLKQLKPDILQDSRLVNLHLTMLVTFTDTSTWKILRGKGETLRPAMNHICANIMGHLNQKGFYSVLQILLTNGLARSRPSLSKGSLTAIFSLALRPVVAAQFSDNLLRSFLIHVMSVPAIMTHLATLTPERLAVIESHELFRKFILFLSRESQCRDVCVCLEGSHTLCLLGNLVFLGFLNDQVLEEETAHFVGVLIHMLSYCQKYVSQKKSNLTHWHPVLGWFSQTVDYGLNESMPLLTKQLQHLWGVHMIRILFSDVLSKKLLENQEIAQLPAQPVSPQNSLPMKNLFKRAFQKSASVRNILKPVGGKRVDSAEVQKVCSICVLYQTTLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPQGGLKLFLECLNNDTEESKRLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENARGETLELFHSVHGWLMVLYERDCRRRFAPEDHWLRKDLKPSVLFQELDKDKKRAQLLLQYIPHVIPHKNRVLLFRNMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQNAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKKVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDISDLGLTLSYDEDVMGQLVCHELVPGGKTIPVTNENKISYIHLMAHFRMHTQIKSQTAALISGFRSIIKPEWIRMFSAPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILANDFSPEERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKKEPGGRLPTSSTCFNLLKLPNYSKKSILREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_010398282.3
Organism	Corvus cornix cornix
Definition	Corvus cornix cornix ubiquitin protein ligase E3B (UBE3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010398282.3

ORF Insert Sequence:

ATGTTCAGTG CCAGCCAGTC GTCCAGGGCT CAGTTCCTGG ACAAGGCCCG GCAGGCGCGG 
GAGGAGCGGC GGGAGCTCAA GGAGAGGGAG AGAGCTGCAG TCCAGCTCCA GGCTCTGGTC 
AGGAGGTTCC TGTGTCGATG CCGCCTGCAG AGGGAGATCA GGAGAGAGGT GGAGGATTTC 
TTTGGGACAA ATGAATGTGG CTCAAGTAAG AGAAGTGCCC TGTCTGTGTT CAGAATTGCC 
AGGAAGCTGC TGTTTGTGTT CAATCCCAAG GAGGACAAGG AGAGGTTTGA AAAGCTGTGC 
CGTTGTATCC TGAACAGCAT GGATGTGGAG AATGAGCCCA AGGTGTGGTA CGTGTCACTG 
GCACTCTCCA AGGACCTCAC ACTCCTCTGG ATCAAGCAGA TCAAAGACAT CCTGTGGTTT 
TGCTGTGAGT TCCTCAAGCA GCTCAAGCCT GATATCCTGC AGGACTCCAG GCTGGTGAAC 
TTGCACCTCA CAATGCTGGT CACCTTCACA GACACTTCCA CCTGGAAAAT CCTCCGGGGA 
AAAGGTGAAA CCCTGAGACC TGCCATGAAC CACATCTGTG CCAACATCAT GGGCCACCTG 
AACCAGAAGG GCTTCTACTC CGTGCTGCAG ATTTTGCTAA CTAATGGCTT GGCAAGATCC 
AGACCTTCCC TGTCCAAAGG CTCCTTAACT GCCATCTTCT CGCTTGCCTT GCGCCCTGTG 
GTTGCTGCAC AGTTCTCAGA CAATCTGCTG AGGTCCTTCC TGATCCATGT CATGTCTGTG 
CCTGCTATAA TGACTCACCT TGCTACTCTC ACCCCTGAGC GCCTGGCAGT GATCGAATCC 
CACGAGCTCT TCCGGAAGTT CATCCTGTTT CTGAGCCGAG AATCCCAGTG CCGGGATGTC 
TGCGTGTGCC TGGAGGGCAG TCACACTCTC TGCTTGCTGG GCAATCTGGT GTTCCTGGGC 
TTCCTGAATG ACCAGGTGCT GGAGGAGGAG ACAGCTCATT TTGTGGGGGT GCTCATCCAC 
ATGCTCTCCT ACTGCCAGAA GTACGTGTCC CAGAAGAAAT CCAACCTCAC CCACTGGCAC 
CCTGTGCTGG GATGGTTCTC ACAGACTGTG GATTATGGGC TGAACGAGTC CATGCCCCTG 
CTGACCAAGC AGCTGCAGCA CCTCTGGGGA GTCCACATGA TCCGTATCCT CTTCAGTGAT 
GTGCTCAGCA AGAAACTGCT GGAAAATCAG GAGATAGCTC AGCTGCCAGC ACAGCCAGTC 
TCCCCCCAGA ACAGCCTTCC TATGAAAAAC CTGTTCAAGA GAGCTTTCCA GAAGTCGGCC 
TCAGTCCGGA ACATCCTGAA GCCGGTGGGA GGGAAGCGCG TGGACTCTGC CGAGGTGCAG 
AAGGTTTGCA GCATCTGTGT CCTGTACCAA ACCACCCTGA CCACCCTCAC CCAGATCCGC 
CTGCAGATCC TCACAGGTCT CACTTACCTG GATGATCTGC TGCCCAAATT ATGGGCTTTC 
ATCTGTGAGC TGGGACCACA GGGGGGGTTA AAACTCTTCT TGGAGTGTTT GAACAACGAC 
ACAGAGGAAT CCAAGAGGCT GCTGGCCATG TTGATGCTCT TCTGTGACTG CTCCCGTCAC 
CTCATCACGA TTCTGGATGA CATCGAGGTC TACGAAGAGC AGATTTCATT CAAACTGGAA 
GAGCTGGTGA CCATCTCCTC TTTCCTGAAT TCCTTTGTGT TTAAGATGAT CTGGGATGGA 
ATTGTAGAGA ATGCCCGAGG GGAGACCCTG GAGCTGTTCC ACTCTGTCCA TGGCTGGCTC 
ATGGTGCTGT ACGAGCGGGA TTGTCGCCGG CGCTTCGCTC CCGAGGATCA CTGGCTGCGC 
AAGGACCTCA AACCCAGCGT GCTCTTCCAG GAGTTGGACA AGGACAAGAA ACGAGCCCAG 
CTGCTGCTGC AGTACATCCC ACACGTCATT CCCCATAAGA ACAGGGTGCT GCTCTTCCGG 
AACATGGTCA CAAAGGAGAA GGAGAAGCTG GGGCTGGTGG AGACGAGCTC GGCTTCCCCT 
CACGTCACAC ACATCACCAT CCGCCGCTCC CGCATGCTGG AGGATGGATA CGAGCAGCTC 
CGGCAGCTTT CCCAGAACGC CATGAAGGGA GTGATCAGGG TGAAGTTTGT CAATGATCTG 
GGGGTTGATG AGGCTGGTAT TGATCAAGAT GGAGTCTTCA AAGAGTTCCT GGAAGAGATC 
ATTAAAAAGG TGTTTGACCC TGCACTGAAT CTGTTCAAGA CCACCAGTGG TGATGAGAGG 
CTGTATCCAT CCCCAACATC CTACATCCAT GAGAACTACC TGCAGCTCTT TGAGTTCGTG 
GGGAAGATGC TTGGGAAGGC TGTGTATGAG GGAATAGTTG TGGATGTACC ATTTGCTTCC 
TTCTTTCTGA GTCAGCTCCT CGGGCACCAC CACAGTGTCT TCTACAGCTC CGTGGATGAA 
CTGCCCTCCT TGGACTCGGA GTTCTATAAA AATCTCACTT CAATTAAGCG TTATGATGGT 
GATATCAGTG ACCTGGGCTT GACACTGTCC TATGATGAAG ATGTGATGGG TCAGCTGGTT 
TGCCATGAAC TTGTTCCTGG AGGGAAGACC ATTCCCGTTA CCAATGAAAA TAAGATCAGC 
TACATCCACC TGATGGCCCA TTTCCGGATG CACACCCAGA TCAAGAGCCA GACTGCAGCT 
CTCATCAGCG GCTTCAGGTC CATCATCAAG CCCGAGTGGA TCCGGATGTT TTCCGCGCCG 
GAGCTGCAGC GCCTCATCTC CGGGGACAAC GCCGAGATCG ACCTCGAGGA CCTGAAGAAG 
CACACGGTGT ACTACGGGGG CTTCCATGGC AGTCACCGCG TCATCATCTG GCTCTGGGAC 
ATCCTGGCCA ACGACTTCAG CCCTGAGGAG AGAGCCATGT TCCTCAAGTT TGTCACCAGC 
TGCTCCAGGC CTCCACTTCT GGGCTTTGCC TACCTCAAGC CTCCTTTCTC CATCCGCTGT 
GTGGAAGTGT CTGATGATCA GGACACGGGG GACACCCTGG GCAGCGTTCT GCGCGGCTTC 
TTCACCATCC GCAAGAAGGA GCCGGGCGGG CGCCTCCCGA CCTCCTCCAC ATGTTTCAAC 
CTCCTCAAGC TCCCCAACTA CAGCAAGAAG AGCATCCTGC GGGAGAAGCT GCGCTACGCC 
ATCAGCATGA ACACCGGCTT CGAGCTGTCC TAG