n cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	n
Entrez Gene ID	723806
Full Name	Notch
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001044360.1	NP_001037825.1	Notch precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Transcriptional regulation of the peripheral nervous system in Ciona intestinalis.
Joyce Tang W, Chen JS, Zeller RW
Developmental biology378(2)183-93(2013 Jun)

Neural tube patterning by Ephrin, FGF and Notch signaling relays.
Stolfi A, Wagner E, Taliaferro JM, Chou S, Levine M
Development (Cambridge, England)138(24)5429-39(2011 Dec)

Interaction of notochord-derived fibrinogen-like protein with Notch regulates the patterning of the central nervous system of Ciona intestinalis embryos.
Yamada S, Hotta K, Yamamoto TS, Ueno N, Satoh N, Takahashi H
Developmental biology328(1)1-12(2009 Apr)

A signalling relay involving Nodal and Delta ligands acts during secondary notochord induction in Ciona embryos.
Hudson C, Yasuo H
Development (Cambridge, England)133(15)2855-64(2006 Aug)

Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.
Imai KS, Hino K, Yagi K, Satoh N, Satou Y
Development (Cambridge, England)131(16)4047-58(2004 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that the microRNA miR-124, which inhibits Notch signaling in epidermal sensory neurons (ESNs), is activated downstream of all the proneural factors, suggesting that these genes operate collectively in a regulatory network.
Title: Transcriptional regulation of the peripheral nervous system in Ciona intestinalis.

It was shown that the developing motor ganglion is patterned by sequential Ephrin/FGF8/MAPK and Delta/Notch signaling events. FGF8/MAPK attenuation by a localized Ephrin Ab signal specified posterior neuronal subtypes.
Title: Neural tube patterning by Ephrin, FGF and Notch signaling relays.

Laterally produced Nodal acts to specify the secondary notochord precursor.
Title: A signalling relay involving Nodal and Delta ligands acts during secondary notochord induction in Ciona embryos.

The following n gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the n cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCi13596	NM_001044360.1 Latest version!	Ciona intestinalis Notch (n), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

n ( NM_001044360.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> NP_001037825.1 Ciona intestinalis Notch (n), mRNA.

Price & Availability↑

CloneID	OCi13596
Clone ID Related Accession (Same CDS sequence)	NM_001044360.1
Accession Version	NM_001044360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-28
Organism	Ciona intestinalis(vase tunicate)
Product	Notch precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB231293.1. ##Evidence-Data-START## Transcript exon combination :: AB231293.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028078, SAMD00028079 [ECO:0000348] ##Evidence-Data-END##

ATGGGATTAA AAGTTCTAAT GTTTTGCACA CTAGTTGCAC TTCTTATATT TTCATTTGCT 
GGAAACTGCA AAGGTGTTTC ATGTTCATTG AATCCTTGTT TAAATGGTGG GGAATGTATA 
ACACATTATG ATGGGAATGA CATATGCAGG TGTGCGCGTG GTTACGGGGG AGCTACGTGC 
AACGTACAGG ATACCACATG TGATGTATCG CATTGTAACA ACGGTGGTAC ATGCCATACT 
GCAGTAGAGG ACGGCCGCAC CGTTCAAATG TGTTTATGTA CCTTCGCATA CACCGGTGAT 
ACTTGTCAAG TGCTGATAAG TTCGTGCGAG GAAGTGGATT GTGTGCAAGG CGAATGCGAA 
ATACAAGACG GTCGGGCGGT GTGTGTTTGT GAGGAAAATT ACACAGGCCC AAGATGCAAC 
AACTATGATT ATTGCTCCAG TGAGCCATGT AGGAACGGGG CAACATGCAC CAACCTGCCA 
CAGCACTTCA GCTGTACATG TGCAGAATAC TGGTTCGGCG ACACGTGCGA AAATTTCCGA 
AGTCCTTGTA TATCAGTGCC CAATATATGC AAGAATAATG GTGTTTGCGA GACTACAGAT 
GATCATTGGT ATTGTGTTTG TCCCAATGGT TTTGTGGGCA ATTATTGTGA AGCCAAGAAC 
ATTCAACGCA GCTCAGACCC ATGTACACCT GATCCTTGTG TTAATAGTGG TACATGCGTG 
GAGAAGTCAG CCACAGTGTA CCAGTGTGTG TGTTTACCTG GGTTTGGAGG AACCAACTGT 
GAAATAGACA TTGACGACTG CCAAGATGAT CCTTGTCATA ATGGAGCTAC ATGTATTGAC 
CAAGCTAACT CACCGTTGTG TCAGTGTGCC CCTGGGTGGA CAGGTGCTAC ATGCGAACAT 
GGCGTGGACG AATGCGCACT AAATCCATCA GCATGTTTAC ATGGGGGAAC ATGTCTCAAC 
TCAGAAGGTT CCTACCAATG TACTTGTGTT AATGGTTGGA CAGGTGAAGA TTGTGGAACA 
AACGAGGACA ATTGTTTAAA TAAACCTTGT TTTCATGGAG CTACATGTAT TGACTTGGTG 
GGAAGATTTG AATGCATTTG TCCTCTTAAT AAAACAGGAT TGTTATGCCA TGTTGACAAC 
GCTTGTGTGG CGGACCCTCC CCCTTGCGCA GCGGGTGCAC AATGCGACAC CAACCCAATA 
AACGGGCAAG CGATATGCAC GTGCCCTGCA GGTTACACTG GTGTTAATTG CTCGCTTGAT 
CTGAACGAAT GTGACTTGGG AACGAACCCA TGTGAATATG ATGGCACATG CGTTAACATA 
CCGGGCAGCT ACAAGTGTGT GTGCGCTAAT GGTTATTCTG GCCCAAGATG TGAGGAAAAT 
ATAAACGAAT GCGAATCCAA CCCATGTGTT AATGACGCAA CTTGTCTGGA TAAGATTGGA 
ACGTTTCTAT GTCTTTGCTT GCCAGGTTAT ACTGGGGTAA CATGCAGTGA AGAAATTGAT 
GAATGCGAAT CAAACCCTTG TCTTTATGAT GGAGAATGCA TTGACCAAGT GCATGGATAT 
ATCTGTAACT GTGCAGCAGG ATACACTGGT ACAAACTGTC AAACCAACAT AGATGATTGC 
GCGTTGGCAC ATTGTCAGAA CCACATAGAA TGTGTAGATG ATGTGGACGA CTATACTTGC 
ATTTGTCTAC CTGGATATGG TGGTAGGAAT TGTGAAACGG ATATTGATGA ATGTGCATCA 
TCTCCCTGCC ATTATGGTAC TTGTGTAGAT GGAATTAACA GATACACATG TAACTGTGAG 
CTTGGATATG GTGGTACATT ATGTGACACC CCGATAAATG AATGCGACTC CAACCCATGT 
CAGAATGGTG GCACGTGCCA CGATCTTGTG GCTTCGTATT CATGCGACTG CCCTCTTGGT 
ACCGCAGGAT CTAATTGCGA AACCAACATT CAAGAATGTG CGTCCAATCC TTGTCAACAT 
GGTCAATGTG TTGATGGTAT CAACAAATAC ACATGTGACT GTGATCCTGG CTATTCAGGC 
CTTCATTGTG GAGCAGATAT CAACGAATGT TTATCCAACC CATGTTATAA TGGGGGCACT 
TGCGAAAATC GACTCAATTC ATTTGTGTGT CATTGCCCTG CAGGTTATAA CGACCCAAGA 
TGTTATTCCA ACATTGATGA ATGCATGAGC AATCCCTGTG TGAATGGAGA TTGCCAGGAT 
GACATCAACA AGTATATATG CAACTGCTAC CATGGGTATG TGGGTGTCAA CTGTGAGTAC 
GATATTAATG AATGCGAGTC ATCACCTTGC GTTAATGGAG GTTCATGCTT GGATCTTGTC 
AACTCATATT CATGCAGATG CAAGACTGGA TATGAAGGTG TAAATTGTGA AACTGATATT 
GATGAATGTA GTCCATCACC TTGTTTAAAT GGTGGTTCAT GTGCAAACCT TATTGGTCGA 
TATGTTTGTA CTTGTCCACT GGGATTCACA GGTAGCGAAT GTGAAACAGC TTTAACCCCA 
TGTGACCCCA TACCATGTAA TAATGGTGGT AGTTGCCAAC CTAATGCTGA CTACCTTGGC 
TACACATGTG TTTGTACACC AGGATTTCAA GGCCCCACAT GCGCAAATGA CATCAATGAA 
TGCATGTCCC CACCCTGTAA GAATGGTGGC AAATGTAGAA ACCGGGAACC CGGTTATTTC 
TGTGAATGTT TGGATGGTTA TTCCGGCGTA AACTGTGAGG AGAACGTGGA TGATTGTGCT 
AGTGACCCAT GCATGAACGG TGGAACTTGC CTTGATGATG TTAATTCGTA TAAATGTCTT 
TGTAAACGTG GTTTTGACGG CAACCAATGT CAGAACGATG TGAATGAATG TGAGAATGAA 
CCATGTAAGA ATGGAGCTAC GTGTACAGAT TATGTAAACA GCTACGCTTG TACTTGCCCG 
CCTGGTTTTC GTGGAACAAC ATGTATGGAA AACATTGATG AATGTAATAT TGGTTCATGT 
TTAAATGGTG GAACATGTGT GGACGGGATT AATTCATATT CATGCAACTG TATGGCTGGT 
TTCACAGGTG CCAACTGTGA GCGCGATATA GATGAATGTG TTTCCTCCCC GTGTAAGAAT 
GGAGCACCAT GCATTCATGG TATCAATACA TTCACATGCC AGTGTTTGAC CGGCTATACT 
GGACCCACAT GTGCTCAAAT GGTTGATTTG TGCCAGAACA ACCCATGTAG GAATGGTGGA 
CAATGTAGTC AAACAGGAAC TACATCTAAA TGCTTGTGTA CATCATCGTA TTCTGGTGTT 
TATTGCGATG TACCTCGGTT GTCGTGCTCT GCTGCTGCTA CATGGCAAGG TGTTGAAGAG 
ACATCATTAT GCCAACATGG TGGGCAATGC ATCAACAGTG GCTCGACACA TTATTGCTCA 
TGTAGGGCAG GTTATGTGGG AAGTTATTGT GAAACAGATG AAGATGATTG TGCGAGCTAC 
CCTTGTAAGA ATGGAGCGAC GTGTACTGAC TATCCTGGAA GCTACACATG TACATGCATG 
GATGGATTCC AAGGAACTAG ATGTGAAACT GAACTGAATG AATGCGAATC TAACCCATGT 
TTAAACAACG GACAATGTGT TGATTTGATC AACGCTTATA AATGTTTATG TCAAGTAGGG 
ACCAGTGGTG ATCGTTGCCA AGACAATGAG AATGAATGTA CCAGCACTTC TTGCTTCAAT 
AGTGGGAACT GTGTGGATGG GATTGGTGGA TTCACATGTA CCTGTGTTCC TGGTTATGCT 
GGCCCAAGAT GCGAGGGTGA CGTGAATGAA TGTGTGCCAA ACCCATGTAA TACTGAAGGT 
TCAACTAACT GTGTACAAGG GATAAATAAG TTTACGTGTA CATGTAAACA GGGATATACT 
GGCACGAGGT GTGAGAAAAA ACTTACAAGT TGCAGCACTA TGCCTTGTTT TAATGGAGCC 
ACATGTACAG ATGCTCCGTA TGAACCCACT GGGTATAGAT GTACTTGCTC TAATGGTTAT 
CGCGGCACCA ACTGTGAGGA AGTGATGTCA CGATGTAGAG ATGGGCTTGT GTGTTACAAC 
GGGGGTACAT GCCATGAAAA CGCAAGTGGT GGCGAAATGT GTWTGTGTCC TGCTGACTAT 
AATGGTGACA ACTGTATATA CTACATACCT GGTCAAGCTG CAGGTCCTGG GACCAATAAT 
ATCAATATAC CTGAGTTTAT AAAGAAAGTA TGTCTGGATC CCACATGCGC TGCATTGAAA 
GGAAATGGGG AATGTGATGC GAAATGTAAC ACCCACGGTT GTGAGTACGA TGGGGGAGAT 
TGTTCTTACG GAAATACCGA TCCTTGGGCA AAATGTCCGG CATCTTTACA ATGTTACAAT 
TACTTTCACG ATGGTGCATG CGATGAAGCG TGTAATAATG CTGATTGTTT GTTTGACGGC 
TTCGATTGTG AAACGCCACA TGAAGCTTGC CCAGCCGATT ACCAAGATTA TTGCGATAAG 
AATTACAAGA ATAATAAATG CGACAGCGGT TGTAATACAT TTGGGTGTGG TTGGGACGGG 
TTGGATTGCG ATAAAGATCA TCCGCAACAA CCTGCAGCTG GAGAACTGAT AATGATCGTC 
CTTATACCAC CTGAGGAATT GATCTCGCGC CAAGCAGAGT TTCTTCGGTT TATTGGAACT 
GTGCTTCACT CTGTTGTTGA GTTTAAACTA ACAACATCAG GACAACCTTA CATTGAAGCA 
ATATCTTCAC AGTTATCACG AAAGAAAAGA TCGGTAATAT CTTCGCTTGG TTGGGAGAAG 
TTTGTCGGAT ATGATTCATC CATTTATCAT AACAAAACAC ACACTCGATT ACGAAGAGCT 
TCAAACACTT TCTTCTCTAA GGCTCACTTA TTGGTAAACA GCCAGAAATG CAAGCAAACA 
TCATCTGAAT GCATTGATAC GACTGACAAC GCAGCTTCTG TGATATCAGC GCGTGCAAGC 
ACAGGAACGT TGGAAGCCGA GTTTCCCATC AATACTGTAG CCAGCACTAC CAACCCAACC 
CCCCCACCAC AGGATTATAC ATACATAGTT GGGGCAGCAG GAGGAGTGGT TCTCGTGATC 
GTGATCATTG GTGTTCTGAC GCACCGCAAG CGAAAGCGTG AGACAAGTAC ATTATGGGCA 
CCCGAAGGTT TCAATGTTAC CAAGAAACAA AGGAGAGAAC CCATCGGACA AGATGACTTG 
AATGGTTTAA ACGGCAGCAT CCACCCCGGC GAGCTTACCC AGCTTGATAC AGCAGGAACC 
CCGTTTTTAA ATCGATGGGA AAACACCTCG TTGCCCCAGA AATCMAACCA CTACCATGTA 
CAATACACAC CAGAGAATAT TACATTTCTT CCTAACAACG GCACTGTGCC ACGCTCACAA 
ATGTCGAGCT CGGCGCAAAA ATCGTCGAGT CGCGACGACA TGGAACCCAC CGACAATCGC 
AAGTGGACGC CACAACATCT GGAAGCTGCG GATTTATCAC GAGCGGGGTC CGCTTGTACC 
CCCGTTACCG ACCTCACCCC ACCCCCGCAT ATAGATGTGG ATGAAGATGA TGTTAACGCA 
CGCGGTCCAG ATGGAGTAAC TCCATTGATG GTTGCCTCCA TACGTGGTGG GGGGGTTGAC 
CATGGTATTA GTGACGATGA ATCCCAACAT AGTGGTGATG CTGGTATAAG TGGTGAAGGA 
AGCGATTCCA TGATTGCTGG TCTTCTACTG CAAGGAGCAT CATTGAGCGC CCAAACAGAT 
AGATCTGGTG AAACTGCATT GCACTTGGCT GCGCGTTACG CACGAGCGGA TGCAGCGAAA 
CGACTCCTTG ATGCAGGAGC GGATGCAAAT ATGAAGGACC ACAGTGGGAG GACTCCTCTT 
CATTCTGCTG TGGCTGCCGA CGCACAAGGA GTCTTCCAAA TATTACTCAG AAACAGAGCA 
ACAGACCTTG ATGCAAGAAC AAACGATGGA ACGACACCCA TGATATTGGC ATCTAGATTA 
GCAGTTGAAG GAATGGTGGA AGAATTGATA AGTGCTAATG CTGATGTTAA TGCTGTGGAT 
GATCATGGCA AATCTGCTCT CCACTGGGCT GCAGCGGTGA ACAACGTGGA TGCGGTTTCC 
ACGCTCTTGA GAGCTGGATG TAACAGGGAT GCTCAAACTG AGCGCGAGGA GACTCCTCTC 
TTTCTTGCAG CAAAGGAGGG AAGTTACGAA GCCATTCGTA TTTTACTCGA TCATTACGCT 
AACAGGGATA TAACGGACCA TATGGATAGG TTACCCCGTG ACATTGCACA AGAACGCCTC 
CATACAGATA TCGTTCAACT ACTTGATGAA TATAACCTCG TGCGAAGCCC ACCACTGCCG 
GCCAATCACA TGACGTCACA CATGAACGGA CATGCGATGA TGTCACGTGA CCCATACCTC 
ACTTCACAGA TGAAACAACA TACAACGGGC AAGAAACCTC GTCGTCAATC AAATCGTGCG 
GATGGTAAAC CTTCTCGGAG GGTAAAAAAA ACCCCTACAA GCTCTCAAGG CCCCCCATCA 
TCTGTTACCA CTGCCGATAG CATCACCCCT GTAATTGACC CCACCCCAAA TATGGCAATT 
CACCCCGGGA TCACCCACGT GGGTGAAAGC ACCCTCTCAC CCGGCAACAA CTCCCTGCCT 
TCACCCGCTA CCACGTATAG CACCGACTCC ACCCTGTCAC CCCCGTCTTC CATGGGCCCC 
CCATCGAACG GGACGTACCA CCCCCACCTA CACCCCCACC AACAAGGGGT TCCGCACCAG 
TTCAGCAACA TGGAAGAGCG AATGATGTCA ACGCTCAACT ACCGGGGAAC CCCCCCACGC 
CTCTCACACC CCCTACCGCA CGGTGGGGCA CCAGGGTTAT ACCCCGCACG ATCACACGGA 
CACCTTGCAG GCACCCACAC TGGCCTCGGG TTCTCACTTG GCCAAATACC CAACGGACAG 
GTCCCACAAG AGTGGATACA ACGCAATGAA AGAATTCAAC AACAGAATCA ACAAAATCAA 
CAGATGTACA ACGCAGTGAT CAGCCAATCA GGCGTCCACC ACCCGTCACA GTTCGCCGCA 
TATCGTGCCC TTGGTTTCCC CGAGCAACAA CATAACAGGG TGGATAGCGC CTCTAGTGAC 
CAAAGAAGAT ATTCTGAAGT GATATACGGC AAAGGGTTGC CTATATACAG TCTACCACAC 
ACCCAACCCC AGCTAGGCTA CACCACTATA CAGGGCGTAC CACACCAACA CCCAGTAAGC 
TCTGCAGGAC AGACACCTCC AGTGCAAACT GGCACAGTTA TAGCACCACA TAGTTACACC 
AATGTATTGG AGAAATATCC GACCCCCCCA TCTGCGCATA CGATGTCTCC TTCATCTGGA 
TCGGAGAACC TTTCACCCCC TGGACCATTA CCCCCTGCCC CCAAGCATTT GGTGCACGAT 
CTCTCATACG AAACCCCCAG CCCCGATTCT CCCGACCCAT GGTCCAGCTC TTCACCCAGC 
AACTCAGACT GGTCTGAGGG TATCCGTAGC CCCCCAGGCC CCATGCAGGG TCACCACCAT 
CATGCCCCGC AACCACAGCA CCAGAGAATG CGAGTTTACC CACCACAGAA CACCGGGGCT 
GCGATCGCCG GCAAAATGGG GGTAGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001037825.1
CDS	1..7650
Translation	MGLKVLMFCTLVALLIFSFAGNCKGVSCSLNPCLNGGECITHYDGNDICRCARGYGGATCNVQDTTCDVSHCNNGGTCHTAVEDGRTVQMCLCTFAYTGDTCQVLISSCEEVDCVQGECEIQDGRAVCVCEENYTGPRCNNYDYCSSEPCRNGATCTNLPQHFSCTCAEYWFGDTCENFRSPCISVPNICKNNGVCETTDDHWYCVCPNGFVGNYCEAKNIQRSSDPCTPDPCVNSGTCVEKSATVYQCVCLPGFGGTNCEIDIDDCQDDPCHNGATCIDQANSPLCQCAPGWTGATCEHGVDECALNPSACLHGGTCLNSEGSYQCTCVNGWTGEDCGTNEDNCLNKPCFHGATCIDLVGRFECICPLNKTGLLCHVDNACVADPPPCAAGAQCDTNPINGQAICTCPAGYTGVNCSLDLNECDLGTNPCEYDGTCVNIPGSYKCVCANGYSGPRCEENINECESNPCVNDATCLDKIGTFLCLCLPGYTGVTCSEEIDECESNPCLYDGECIDQVHGYICNCAAGYTGTNCQTNIDDCALAHCQNHIECVDDVDDYTCICLPGYGGRNCETDIDECASSPCHYGTCVDGINRYTCNCELGYGGTLCDTPINECDSNPCQNGGTCHDLVASYSCDCPLGTAGSNCETNIQECASNPCQHGQCVDGINKYTCDCDPGYSGLHCGADINECLSNPCYNGGTCENRLNSFVCHCPAGYNDPRCYSNIDECMSNPCVNGDCQDDINKYICNCYHGYVGVNCEYDINECESSPCVNGGSCLDLVNSYSCRCKTGYEGVNCETDIDECSPSPCLNGGSCANLIGRYVCTCPLGFTGSECETALTPCDPIPCNNGGSCQPNADYLGYTCVCTPGFQGPTCANDINECMSPPCKNGGKCRNREPGYFCECLDGYSGVNCEENVDDCASDPCMNGGTCLDDVNSYKCLCKRGFDGNQCQNDVNECENEPCKNGATCTDYVNSYACTCPPGFRGTTCMENIDECNIGSCLNGGTCVDGINSYSCNCMAGFTGANCERDIDECVSSPCKNGAPCIHGINTFTCQCLTGYTGPTCAQMVDLCQNNPCRNGGQCSQTGTTSKCLCTSSYSGVYCDVPRLSCSAAATWQGVEETSLCQHGGQCINSGSTHYCSCRAGYVGSYCETDEDDCASYPCKNGATCTDYPGSYTCTCMDGFQGTRCETELNECESNPCLNNGQCVDLINAYKCLCQVGTSGDRCQDNENECTSTSCFNSGNCVDGIGGFTCTCVPGYAGPRCEGDVNECVPNPCNTEGSTNCVQGINKFTCTCKQGYTGTRCEKKLTSCSTMPCFNGATCTDAPYEPTGYRCTCSNGYRGTNCEEVMSRCRDGLVCYNGGTCHENASGGEMCnullCPADYNGDNCIYYIPGQAAGPGTNNINIPEFIKKVCLDPTCAALKGNGECDAKCNTHGCEYDGGDCSYGNTDPWAKCPASLQCYNYFHDGACDEACNNADCLFDGFDCETPHEACPADYQDYCDKNYKNNKCDSGCNTFGCGWDGLDCDKDHPQQPAAGELIMIVLIPPEELISRQAEFLRFIGTVLHSVVEFKLTTSGQPYIEAISSQLSRKKRSVISSLGWEKFVGYDSSIYHNKTHTRLRRASNTFFSKAHLLVNSQKCKQTSSECIDTTDNAASVISARASTGTLEAEFPINTVASTTNPTPPPQDYTYIVGAAGGVVLVIVIIGVLTHRKRKRETSTLWAPEGFNVTKKQRREPIGQDDLNGLNGSIHPGELTQLDTAGTPFLNRWENTSLPQKnullNHYHVQYTPENITFLPNNGTVPRSQMSSSAQKSSSRDDMEPTDNRKWTPQHLEAADLSRAGSACTPVTDLTPPPHIDVDEDDVNARGPDGVTPLMVASIRGGGVDHGISDDESQHSGDAGISGEGSDSMIAGLLLQGASLSAQTDRSGETALHLAARYARADAAKRLLDAGADANMKDHSGRTPLHSAVAADAQGVFQILLRNRATDLDARTNDGTTPMILASRLAVEGMVEELISANADVNAVDDHGKSALHWAAAVNNVDAVSTLLRAGCNRDAQTEREETPLFLAAKEGSYEAIRILLDHYANRDITDHMDRLPRDIAQERLHTDIVQLLDEYNLVRSPPLPANHMTSHMNGHAMMSRDPYLTSQMKQHTTGKKPRRQSNRADGKPSRRVKKTPTSSQGPPSSVTTADSITPVIDPTPNMAIHPGITHVGESTLSPGNNSLPSPATTYSTDSTLSPPSSMGPPSNGTYHPHLHPHQQGVPHQFSNMEERMMSTLNYRGTPPRLSHPLPHGGAPGLYPARSHGHLAGTHTGLGFSLGQIPNGQVPQEWIQRNERIQQQNQQNQQMYNAVISQSGVHHPSQFAAYRALGFPEQQHNRVDSASSDQRRYSEVIYGKGLPIYSLPHTQPQLGYTTIQGVPHQHPVSSAGQTPPVQTGTVIAPHSYTNVLEKYPTPPSAHTMSPSSGSENLSPPGPLPPAPKHLVHDLSYETPSPDSPDPWSSSSPSNSDWSEGIRSPPGPMQGHHHHAPQPQHQRMRVYPPQNTGAAIAGKMGVA

Target ORF information:

RefSeq Version	NM_001044360.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis Notch (n), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044360.1

ORF Insert Sequence:

ATGGGATTAA AAGTTCTAAT GTTTTGCACA CTAGTTGCAC TTCTTATATT TTCATTTGCT 
GGAAACTGCA AAGGTGTTTC ATGTTCATTG AATCCTTGTT TAAATGGTGG GGAATGTATA 
ACACATTATG ATGGGAATGA CATATGCAGG TGTGCGCGTG GTTACGGGGG AGCTACGTGC 
AACGTACAGG ATACCACATG TGATGTATCG CATTGTAACA ACGGTGGTAC ATGCCATACT 
GCAGTAGAGG ACGGCCGCAC CGTTCAAATG TGTTTATGTA CCTTCGCATA CACCGGTGAT 
ACTTGTCAAG TGCTGATAAG TTCGTGCGAG GAAGTGGATT GTGTGCAAGG CGAATGCGAA 
ATACAAGACG GTCGGGCGGT GTGTGTTTGT GAGGAAAATT ACACAGGCCC AAGATGCAAC 
AACTATGATT ATTGCTCCAG TGAGCCATGT AGGAACGGGG CAACATGCAC CAACCTGCCA 
CAGCACTTCA GCTGTACATG TGCAGAATAC TGGTTCGGCG ACACGTGCGA AAATTTCCGA 
AGTCCTTGTA TATCAGTGCC CAATATATGC AAGAATAATG GTGTTTGCGA GACTACAGAT 
GATCATTGGT ATTGTGTTTG TCCCAATGGT TTTGTGGGCA ATTATTGTGA AGCCAAGAAC 
ATTCAACGCA GCTCAGACCC ATGTACACCT GATCCTTGTG TTAATAGTGG TACATGCGTG 
GAGAAGTCAG CCACAGTGTA CCAGTGTGTG TGTTTACCTG GGTTTGGAGG AACCAACTGT 
GAAATAGACA TTGACGACTG CCAAGATGAT CCTTGTCATA ATGGAGCTAC ATGTATTGAC 
CAAGCTAACT CACCGTTGTG TCAGTGTGCC CCTGGGTGGA CAGGTGCTAC ATGCGAACAT 
GGCGTGGACG AATGCGCACT AAATCCATCA GCATGTTTAC ATGGGGGAAC ATGTCTCAAC 
TCAGAAGGTT CCTACCAATG TACTTGTGTT AATGGTTGGA CAGGTGAAGA TTGTGGAACA 
AACGAGGACA ATTGTTTAAA TAAACCTTGT TTTCATGGAG CTACATGTAT TGACTTGGTG 
GGAAGATTTG AATGCATTTG TCCTCTTAAT AAAACAGGAT TGTTATGCCA TGTTGACAAC 
GCTTGTGTGG CGGACCCTCC CCCTTGCGCA GCGGGTGCAC AATGCGACAC CAACCCAATA 
AACGGGCAAG CGATATGCAC GTGCCCTGCA GGTTACACTG GTGTTAATTG CTCGCTTGAT 
CTGAACGAAT GTGACTTGGG AACGAACCCA TGTGAATATG ATGGCACATG CGTTAACATA 
CCGGGCAGCT ACAAGTGTGT GTGCGCTAAT GGTTATTCTG GCCCAAGATG TGAGGAAAAT 
ATAAACGAAT GCGAATCCAA CCCATGTGTT AATGACGCAA CTTGTCTGGA TAAGATTGGA 
ACGTTTCTAT GTCTTTGCTT GCCAGGTTAT ACTGGGGTAA CATGCAGTGA AGAAATTGAT 
GAATGCGAAT CAAACCCTTG TCTTTATGAT GGAGAATGCA TTGACCAAGT GCATGGATAT 
ATCTGTAACT GTGCAGCAGG ATACACTGGT ACAAACTGTC AAACCAACAT AGATGATTGC 
GCGTTGGCAC ATTGTCAGAA CCACATAGAA TGTGTAGATG ATGTGGACGA CTATACTTGC 
ATTTGTCTAC CTGGATATGG TGGTAGGAAT TGTGAAACGG ATATTGATGA ATGTGCATCA 
TCTCCCTGCC ATTATGGTAC TTGTGTAGAT GGAATTAACA GATACACATG TAACTGTGAG 
CTTGGATATG GTGGTACATT ATGTGACACC CCGATAAATG AATGCGACTC CAACCCATGT 
CAGAATGGTG GCACGTGCCA CGATCTTGTG GCTTCGTATT CATGCGACTG CCCTCTTGGT 
ACCGCAGGAT CTAATTGCGA AACCAACATT CAAGAATGTG CGTCCAATCC TTGTCAACAT 
GGTCAATGTG TTGATGGTAT CAACAAATAC ACATGTGACT GTGATCCTGG CTATTCAGGC 
CTTCATTGTG GAGCAGATAT CAACGAATGT TTATCCAACC CATGTTATAA TGGGGGCACT 
TGCGAAAATC GACTCAATTC ATTTGTGTGT CATTGCCCTG CAGGTTATAA CGACCCAAGA 
TGTTATTCCA ACATTGATGA ATGCATGAGC AATCCCTGTG TGAATGGAGA TTGCCAGGAT 
GACATCAACA AGTATATATG CAACTGCTAC CATGGGTATG TGGGTGTCAA CTGTGAGTAC 
GATATTAATG AATGCGAGTC ATCACCTTGC GTTAATGGAG GTTCATGCTT GGATCTTGTC 
AACTCATATT CATGCAGATG CAAGACTGGA TATGAAGGTG TAAATTGTGA AACTGATATT 
GATGAATGTA GTCCATCACC TTGTTTAAAT GGTGGTTCAT GTGCAAACCT TATTGGTCGA 
TATGTTTGTA CTTGTCCACT GGGATTCACA GGTAGCGAAT GTGAAACAGC TTTAACCCCA 
TGTGACCCCA TACCATGTAA TAATGGTGGT AGTTGCCAAC CTAATGCTGA CTACCTTGGC 
TACACATGTG TTTGTACACC AGGATTTCAA GGCCCCACAT GCGCAAATGA CATCAATGAA 
TGCATGTCCC CACCCTGTAA GAATGGTGGC AAATGTAGAA ACCGGGAACC CGGTTATTTC 
TGTGAATGTT TGGATGGTTA TTCCGGCGTA AACTGTGAGG AGAACGTGGA TGATTGTGCT 
AGTGACCCAT GCATGAACGG TGGAACTTGC CTTGATGATG TTAATTCGTA TAAATGTCTT 
TGTAAACGTG GTTTTGACGG CAACCAATGT CAGAACGATG TGAATGAATG TGAGAATGAA 
CCATGTAAGA ATGGAGCTAC GTGTACAGAT TATGTAAACA GCTACGCTTG TACTTGCCCG 
CCTGGTTTTC GTGGAACAAC ATGTATGGAA AACATTGATG AATGTAATAT TGGTTCATGT 
TTAAATGGTG GAACATGTGT GGACGGGATT AATTCATATT CATGCAACTG TATGGCTGGT 
TTCACAGGTG CCAACTGTGA GCGCGATATA GATGAATGTG TTTCCTCCCC GTGTAAGAAT 
GGAGCACCAT GCATTCATGG TATCAATACA TTCACATGCC AGTGTTTGAC CGGCTATACT 
GGACCCACAT GTGCTCAAAT GGTTGATTTG TGCCAGAACA ACCCATGTAG GAATGGTGGA 
CAATGTAGTC AAACAGGAAC TACATCTAAA TGCTTGTGTA CATCATCGTA TTCTGGTGTT 
TATTGCGATG TACCTCGGTT GTCGTGCTCT GCTGCTGCTA CATGGCAAGG TGTTGAAGAG 
ACATCATTAT GCCAACATGG TGGGCAATGC ATCAACAGTG GCTCGACACA TTATTGCTCA 
TGTAGGGCAG GTTATGTGGG AAGTTATTGT GAAACAGATG AAGATGATTG TGCGAGCTAC 
CCTTGTAAGA ATGGAGCGAC GTGTACTGAC TATCCTGGAA GCTACACATG TACATGCATG 
GATGGATTCC AAGGAACTAG ATGTGAAACT GAACTGAATG AATGCGAATC TAACCCATGT 
TTAAACAACG GACAATGTGT TGATTTGATC AACGCTTATA AATGTTTATG TCAAGTAGGG 
ACCAGTGGTG ATCGTTGCCA AGACAATGAG AATGAATGTA CCAGCACTTC TTGCTTCAAT 
AGTGGGAACT GTGTGGATGG GATTGGTGGA TTCACATGTA CCTGTGTTCC TGGTTATGCT 
GGCCCAAGAT GCGAGGGTGA CGTGAATGAA TGTGTGCCAA ACCCATGTAA TACTGAAGGT 
TCAACTAACT GTGTACAAGG GATAAATAAG TTTACGTGTA CATGTAAACA GGGATATACT 
GGCACGAGGT GTGAGAAAAA ACTTACAAGT TGCAGCACTA TGCCTTGTTT TAATGGAGCC 
ACATGTACAG ATGCTCCGTA TGAACCCACT GGGTATAGAT GTACTTGCTC TAATGGTTAT 
CGCGGCACCA ACTGTGAGGA AGTGATGTCA CGATGTAGAG ATGGGCTTGT GTGTTACAAC 
GGGGGTACAT GCCATGAAAA CGCAAGTGGT GGCGAAATGT GTWTGTGTCC TGCTGACTAT 
AATGGTGACA ACTGTATATA CTACATACCT GGTCAAGCTG CAGGTCCTGG GACCAATAAT 
ATCAATATAC CTGAGTTTAT AAAGAAAGTA TGTCTGGATC CCACATGCGC TGCATTGAAA 
GGAAATGGGG AATGTGATGC GAAATGTAAC ACCCACGGTT GTGAGTACGA TGGGGGAGAT 
TGTTCTTACG GAAATACCGA TCCTTGGGCA AAATGTCCGG CATCTTTACA ATGTTACAAT 
TACTTTCACG ATGGTGCATG CGATGAAGCG TGTAATAATG CTGATTGTTT GTTTGACGGC 
TTCGATTGTG AAACGCCACA TGAAGCTTGC CCAGCCGATT ACCAAGATTA TTGCGATAAG 
AATTACAAGA ATAATAAATG CGACAGCGGT TGTAATACAT TTGGGTGTGG TTGGGACGGG 
TTGGATTGCG ATAAAGATCA TCCGCAACAA CCTGCAGCTG GAGAACTGAT AATGATCGTC 
CTTATACCAC CTGAGGAATT GATCTCGCGC CAAGCAGAGT TTCTTCGGTT TATTGGAACT 
GTGCTTCACT CTGTTGTTGA GTTTAAACTA ACAACATCAG GACAACCTTA CATTGAAGCA 
ATATCTTCAC AGTTATCACG AAAGAAAAGA TCGGTAATAT CTTCGCTTGG TTGGGAGAAG 
TTTGTCGGAT ATGATTCATC CATTTATCAT AACAAAACAC ACACTCGATT ACGAAGAGCT 
TCAAACACTT TCTTCTCTAA GGCTCACTTA TTGGTAAACA GCCAGAAATG CAAGCAAACA 
TCATCTGAAT GCATTGATAC GACTGACAAC GCAGCTTCTG TGATATCAGC GCGTGCAAGC 
ACAGGAACGT TGGAAGCCGA GTTTCCCATC AATACTGTAG CCAGCACTAC CAACCCAACC 
CCCCCACCAC AGGATTATAC ATACATAGTT GGGGCAGCAG GAGGAGTGGT TCTCGTGATC 
GTGATCATTG GTGTTCTGAC GCACCGCAAG CGAAAGCGTG AGACAAGTAC ATTATGGGCA 
CCCGAAGGTT TCAATGTTAC CAAGAAACAA AGGAGAGAAC CCATCGGACA AGATGACTTG 
AATGGTTTAA ACGGCAGCAT CCACCCCGGC GAGCTTACCC AGCTTGATAC AGCAGGAACC 
CCGTTTTTAA ATCGATGGGA AAACACCTCG TTGCCCCAGA AATCMAACCA CTACCATGTA 
CAATACACAC CAGAGAATAT TACATTTCTT CCTAACAACG GCACTGTGCC ACGCTCACAA 
ATGTCGAGCT CGGCGCAAAA ATCGTCGAGT CGCGACGACA TGGAACCCAC CGACAATCGC 
AAGTGGACGC CACAACATCT GGAAGCTGCG GATTTATCAC GAGCGGGGTC CGCTTGTACC 
CCCGTTACCG ACCTCACCCC ACCCCCGCAT ATAGATGTGG ATGAAGATGA TGTTAACGCA 
CGCGGTCCAG ATGGAGTAAC TCCATTGATG GTTGCCTCCA TACGTGGTGG GGGGGTTGAC 
CATGGTATTA GTGACGATGA ATCCCAACAT AGTGGTGATG CTGGTATAAG TGGTGAAGGA 
AGCGATTCCA TGATTGCTGG TCTTCTACTG CAAGGAGCAT CATTGAGCGC CCAAACAGAT 
AGATCTGGTG AAACTGCATT GCACTTGGCT GCGCGTTACG CACGAGCGGA TGCAGCGAAA 
CGACTCCTTG ATGCAGGAGC GGATGCAAAT ATGAAGGACC ACAGTGGGAG GACTCCTCTT 
CATTCTGCTG TGGCTGCCGA CGCACAAGGA GTCTTCCAAA TATTACTCAG AAACAGAGCA 
ACAGACCTTG ATGCAAGAAC AAACGATGGA ACGACACCCA TGATATTGGC ATCTAGATTA 
GCAGTTGAAG GAATGGTGGA AGAATTGATA AGTGCTAATG CTGATGTTAA TGCTGTGGAT 
GATCATGGCA AATCTGCTCT CCACTGGGCT GCAGCGGTGA ACAACGTGGA TGCGGTTTCC 
ACGCTCTTGA GAGCTGGATG TAACAGGGAT GCTCAAACTG AGCGCGAGGA GACTCCTCTC 
TTTCTTGCAG CAAAGGAGGG AAGTTACGAA GCCATTCGTA TTTTACTCGA TCATTACGCT 
AACAGGGATA TAACGGACCA TATGGATAGG TTACCCCGTG ACATTGCACA AGAACGCCTC 
CATACAGATA TCGTTCAACT ACTTGATGAA TATAACCTCG TGCGAAGCCC ACCACTGCCG 
GCCAATCACA TGACGTCACA CATGAACGGA CATGCGATGA TGTCACGTGA CCCATACCTC 
ACTTCACAGA TGAAACAACA TACAACGGGC AAGAAACCTC GTCGTCAATC AAATCGTGCG 
GATGGTAAAC CTTCTCGGAG GGTAAAAAAA ACCCCTACAA GCTCTCAAGG CCCCCCATCA 
TCTGTTACCA CTGCCGATAG CATCACCCCT GTAATTGACC CCACCCCAAA TATGGCAATT 
CACCCCGGGA TCACCCACGT GGGTGAAAGC ACCCTCTCAC CCGGCAACAA CTCCCTGCCT 
TCACCCGCTA CCACGTATAG CACCGACTCC ACCCTGTCAC CCCCGTCTTC CATGGGCCCC 
CCATCGAACG GGACGTACCA CCCCCACCTA CACCCCCACC AACAAGGGGT TCCGCACCAG 
TTCAGCAACA TGGAAGAGCG AATGATGTCA ACGCTCAACT ACCGGGGAAC CCCCCCACGC 
CTCTCACACC CCCTACCGCA CGGTGGGGCA CCAGGGTTAT ACCCCGCACG ATCACACGGA 
CACCTTGCAG GCACCCACAC TGGCCTCGGG TTCTCACTTG GCCAAATACC CAACGGACAG 
GTCCCACAAG AGTGGATACA ACGCAATGAA AGAATTCAAC AACAGAATCA ACAAAATCAA 
CAGATGTACA ACGCAGTGAT CAGCCAATCA GGCGTCCACC ACCCGTCACA GTTCGCCGCA 
TATCGTGCCC TTGGTTTCCC CGAGCAACAA CATAACAGGG TGGATAGCGC CTCTAGTGAC 
CAAAGAAGAT ATTCTGAAGT GATATACGGC AAAGGGTTGC CTATATACAG TCTACCACAC 
ACCCAACCCC AGCTAGGCTA CACCACTATA CAGGGCGTAC CACACCAACA CCCAGTAAGC 
TCTGCAGGAC AGACACCTCC AGTGCAAACT GGCACAGTTA TAGCACCACA TAGTTACACC 
AATGTATTGG AGAAATATCC GACCCCCCCA TCTGCGCATA CGATGTCTCC TTCATCTGGA 
TCGGAGAACC TTTCACCCCC TGGACCATTA CCCCCTGCCC CCAAGCATTT GGTGCACGAT 
CTCTCATACG AAACCCCCAG CCCCGATTCT CCCGACCCAT GGTCCAGCTC TTCACCCAGC 
AACTCAGACT GGTCTGAGGG TATCCGTAGC CCCCCAGGCC CCATGCAGGG TCACCACCAT 
CATGCCCCGC AACCACAGCA CCAGAGAATG CGAGTTTACC CACCACAGAA CACCGGGGCT 
GCGATCGCCG GCAAAATGGG GGTAGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptional regulation of the peripheral nervous system in Ciona intestinalis.
Developmental biology378(2)183-93(2013 Jun)
Joyce Tang W,Chen JS,Zeller RW

Neural tube patterning by Ephrin, FGF and Notch signaling relays.
Development (Cambridge, England)138(24)5429-39(2011 Dec)
Stolfi A,Wagner E,Taliaferro JM,Chou S,Levine M

Interaction of notochord-derived fibrinogen-like protein with Notch regulates the patterning of the central nervous system of Ciona intestinalis embryos.
Developmental biology328(1)1-12(2009 Apr)
Yamada S,Hotta K,Yamamoto TS,Ueno N,Satoh N,Takahashi H

A signalling relay involving Nodal and Delta ligands acts during secondary notochord induction in Ciona embryos.
Development (Cambridge, England)133(15)2855-64(2006 Aug)
Hudson C,Yasuo H

Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.
Development (Cambridge, England)131(16)4047-58(2004 Aug)
Imai KS,Hino K,Yagi K,Satoh N,Satou Y