LOC494391 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC494391
Entrez Gene ID	494391
Full Name	eukaryotic translation initiation factor 2-alpha kinase 3
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002128452.4	XP_002128488.1	eukaryotic translation initiation factor 2-alpha kinase 3
XM_002128452.3	XP_002128488.1	eukaryotic translation initiation factor 2-alpha kinase 3

Pathways from BioSystems
Gene Ontology

The following LOC494391 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC494391 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCi01751	XM_002128452.4 Latest version!	Ciona intestinalis eukaryotic translation initiation factor 2-alpha kinase 3 (LOC494391), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OCi01751	XM_002128452.3	Ciona intestinalis eukaryotic translation initiation factor 2-alpha kinase 3 (LOC494391), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC494391 ( XM_002128452.4 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_002128488.1 Ciona intestinalis eukaryotic translation initiation factor 2-alpha kinase 3 (LOC494391), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCi01751
Clone ID Related Accession (Same CDS sequence)	XM_002128452.4 , XM_002128452.3
Accession Version	XM_002128452.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Ciona intestinalis(vase tunicate)
Product	eukaryotic translation initiation factor 2-alpha kinase 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020167.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 22, 2018 this sequence version replaced XM_002128452.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGTG GTGTTACTGG CATTGCTAAG TTTTTGACTT CTGTTGGTTT ATTTTCTATT 
GTAACAGCTG GTTCACCAAA CACAGAAAAT CAATCAAAAG AAAAACCTAA AGCTGTCACC 
CTCTCAAGTT ATTCTGACCC ATTGCTCATT GCAACTACAC TCGATGGTAA AATCTCAGCA 
TTGAACATTG CTGATGGTGG AAAATTGAAG TGGGTTGTGG ATTTGGGTCA TGGACCTTTG 
GTTTCATCAT CTCTTGCAAA ACTTGTTTAT AAAGAAGATG GAAAATATGT GCGATTGATT 
CCTTCATTAC ACGGGGGATT ATTTCGCTTT GATGGAGAAT CTGTCGAGGC TGTCCCATTT 
GATGCTGATC AATTATTAAG CTCATCCTTT CGGTTGGCTG ACGAGACAGT GCTTGTTGGT 
GGGAAAGAGA CAAGCACGAG TGGCATTGAT GCCTTTAGTG GAAGAGTGTT ATACACCTGC 
ACTGCCAATG GATGCGACAA ACAACAAAAT GTCCACGATG CTTCAGATTT GTTGATAGTG 
AAACGACAAC AACAAGTTGT TCGTTCAATC ATCAACAGAA ATGGACACGA AAATTGGAAC 
TTCAGTGTTG GTGAAATCAA CCTGGATTTT TCTCAAGGAG CAAATCTACA TCTGTTAGAG 
GAATCATCTG AACATTCAAA TGAACAATCA TACCAGAAAC AGGACACCAA ACAAACCCAC 
ACTGAGAGTG ATGAATTCTC AATGACGGAT GCTCATTGTA TTAAAGTTGT CGTACCAGAT 
GGAATGGTCT GTCTTGTGAC GAGAAAAAAT GAGCATGTAG TGAAATGGAA GCATAAACTT 
GGTTCTCCTG TAAGTGCAGC ATGGTTATTA GTGGATGGAG AACTGAGAAA ACTTGATTTA 
TTTGATCCAA GTATGGTCCC TGCATTGGAT GAAAGCACAG CGGTGGCAAA GAAGGCACCT 
CAGATTAACA CAATTTATAT GGGATCATAT GACAACCATG TTTACATCCA AGCCTCACCT 
GCTGTACAGA AATCTATTGC TATCATCACT GCAAGTAATA GATACGGCAG TACAAATGCA 
AGACAAATGT CACCCAAGTT CGCCTACCGT CGTTGCCACG GTGATATGAC ACCAGTTGCC 
ATAGAAACCA ACAAGGATGA CCCAACAACA GAGTTAGTGG TAAAAGGAAA ACCTCCGCAA 
AAAGCAAACT GTGTTCATGG GTTGACGCTC TACAATGAGC AGTTTCCTAA TGATGGAGGA 
TATTATTTAC AAGATGACTC GAGTTATTTA TCGAGAATAC AAAGCTCGAA GAGGATGGGA 
AGGAATACTG TTGACTCATT AGAGAATGAT TCATATGACA AATACAACAT AGATATCGTA 
TATTCTTCAT TCATGCACTG GTGGAAGGAA CTTCTTATAT TAACTGTGTC GGTTTCAATT 
TTTGTGCAAT GTCTTATCAA CAAGTGCATG CAAGCCTTCA AGTCTCGATC TACAAATGAA 
ACAACGACAA GTTTATATGA ATCACCACCA GAACATCCCC CTCCTGTTTG TGAGGTGGAG 
AGAGAATACG AATCAAGATT CTTGACTGAT TTTGAACCTT TGGAATGCCT TGGAAAGGGA 
GGATTTGGAA TCGTATTCAA GGCAAGAAAT AAAATGGATG ATTGCACATA TGCTGTGAAA 
CGAATTCTTC TTCCTAACAA TGTAAGTTCT CGGGATAAAG TCTTGCGAGA AGTGCGAGCG 
CTTGCAAAGT TGGACCATCC TGCCATAGTG AGATATTTCA ACTCGTGGAA TGAAGAACCT 
CCAGCAGGGT GGCAACAAAA GAGAGATGCT CACCTTAAAG ATATGAGTGC TTGGAGCAGC 
ACTGCGTTTC CCAACACAAG TGAAAAACCA ATCAGCAAGA GGAATAAACA TTCTACTTCA 
AGTGGGACTG GTCAACAAAG CACAGAATTA TCATCAAGTA ACCACATACA ACATGATGAT 
GATGACGTCA TATTTGGGTG TGGTACAACT CAAGGATTTG TTGTTGATAA GACAACAGAG 
AAGACAAGTG ACAGTGGATT GTTTGATGAT CAACATAACG ATAGTTGGGG TAACAAGGAT 
GAAACATGGG GTGATGAACC CGTTGAAATG AGATCCAACA TAGGGGGAAA GTTTTTCCAA 
AGTGACAGCT CCCTCCCCTC AGTCTCTGGA AGTATCGTAC GAAATCGGAA CAATAATAGT 
TCACCCAGAG CTTCTGTTCC ATTTGGAAGA TACATGATGC AAGATTCGCT GAGTGTTGTG 
TTTGATGATC AAGTTTCAAA TGCAGAGAAT TTCAGAACGA ATAATAAGGT CAATGATAAT 
AAACCACCAA GCTATGAAAG TTTGTTTTCC AACGCTGCTG TAAGAAGACG CCCATCAGCA 
AAAGCAACAG ACACCAGCAC TTCCGAATTA AAGGCAGTGG TTTGTATGCA AGATGCGAGC 
ACAGGACACC AGGGAAGACT TGGTCAGCAT AATGATGAAG ACACCCACCC ACAAGACAAC 
ACAATATATC TTTACATTCA AATGCAACTT TGTAAGAAAG AAAGCTTGAG AGAATGGCTT 
GCTGCTAATG TGGAGAAAAG GGATTTCCAC TACTGCTTGA ATATCTTTCA ACAGGTTGTC 
AGTGCAGTTG CCTATGTACA CGACTCTGGG TTGATTCACC GTGATCTAAA ACCATCCAAT 
GTTCTCTTCT CACTTGATGG GACAATAAAA GTTGGTGACT TTGGGTTGGT AACACACGCT 
GGAGAGGGAG ATCCGTTTTT TAACGTGGGA GAACTACCAG AGGCGATACT TGGTAAAGCA 
GATGACAAAC ATACACAAAG AGTTGGCACG AGAATGTACA TGGCTCCTGA GCAAATGTCC 
AGCAGCACAT ACAGTGAAAA GATCGATATA TTTGCACTTG GTTTGATATT TTTTGAGCTC 
ATTCACTCGT TTGGAACACA AATGGAAAGA ATTCTTCATC TTTCTGATGC AAGAAAACTC 
AAGTTTCCCA TTCAATTTTT AAACAACTAC CCGAAAGAGT CCAAGCTGAC TCATCAAATG 
CTGAGTCACA AAGCAAGTGA CCGACCTTCA GCCAACGAAG TGAATGAACA TGAAGTATTC 
ACAGAAGTTT TGGACTTACA ACAATCAAGG TCCCGGCATC GAACGACGTC ATCCAGAAGT 
TCTGACTCCA CTTCAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002128488.1
CDS	339..3539
Translation	MFGGVTGIAKFLTSVGLFSIVTAGSPNTENQSKEKPKAVTLSSYSDPLLIATTLDGKISALNIADGGKLKWVVDLGHGPLVSSSLAKLVYKEDGKYVRLIPSLHGGLFRFDGESVEAVPFDADQLLSSSFRLADETVLVGGKETSTSGIDAFSGRVLYTCTANGCDKQQNVHDASDLLIVKRQQQVVRSIINRNGHENWNFSVGEINLDFSQGANLHLLEESSEHSNEQSYQKQDTKQTHTESDEFSMTDAHCIKVVVPDGMVCLVTRKNEHVVKWKHKLGSPVSAAWLLVDGELRKLDLFDPSMVPALDESTAVAKKAPQINTIYMGSYDNHVYIQASPAVQKSIAIITASNRYGSTNARQMSPKFAYRRCHGDMTPVAIETNKDDPTTELVVKGKPPQKANCVHGLTLYNEQFPNDGGYYLQDDSSYLSRIQSSKRMGRNTVDSLENDSYDKYNIDIVYSSFMHWWKELLILTVSVSIFVQCLINKCMQAFKSRSTNETTTSLYESPPEHPPPVCEVEREYESRFLTDFEPLECLGKGGFGIVFKARNKMDDCTYAVKRILLPNNVSSRDKVLREVRALAKLDHPAIVRYFNSWNEEPPAGWQQKRDAHLKDMSAWSSTAFPNTSEKPISKRNKHSTSSGTGQQSTELSSSNHIQHDDDDVIFGCGTTQGFVVDKTTEKTSDSGLFDDQHNDSWGNKDETWGDEPVEMRSNIGGKFFQSDSSLPSVSGSIVRNRNNNSSPRASVPFGRYMMQDSLSVVFDDQVSNAENFRTNNKVNDNKPPSYESLFSNAAVRRRPSAKATDTSTSELKAVVCMQDASTGHQGRLGQHNDEDTHPQDNTIYLYIQMQLCKKESLREWLAANVEKRDFHYCLNIFQQVVSAVAYVHDSGLIHRDLKPSNVLFSLDGTIKVGDFGLVTHAGEGDPFFNVGELPEAILGKADDKHTQRVGTRMYMAPEQMSSSTYSEKIDIFALGLIFFELIHSFGTQMERILHLSDARKLKFPIQFLNNYPKESKLTHQMLSHKASDRPSANEVNEHEVFTEVLDLQQSRSRHRTTSSRSSDSTSS

Target ORF information:

RefSeq Version	XM_002128452.4
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis eukaryotic translation initiation factor 2-alpha kinase 3 (LOC494391), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002128452.4

ORF Insert Sequence:

ATGTTTGGTG GTGTTACTGG CATTGCTAAG TTTTTGACTT CTGTTGGTTT ATTTTCTATT 
GTAACAGCTG GTTCACCAAA CACAGAAAAT CAATCAAAAG AAAAACCTAA AGCTGTCACC 
CTCTCAAGTT ATTCTGACCC ATTGCTCATT GCAACTACAC TCGATGGTAA AATCTCAGCA 
TTGAACATTG CTGATGGTGG AAAATTGAAG TGGGTTGTGG ATTTGGGTCA TGGACCTTTG 
GTTTCATCAT CTCTTGCAAA ACTTGTTTAT AAAGAAGATG GAAAATATGT GCGATTGATT 
CCTTCATTAC ACGGGGGATT ATTTCGCTTT GATGGAGAAT CTGTCGAGGC TGTCCCATTT 
GATGCTGATC AATTATTAAG CTCATCCTTT CGGTTGGCTG ACGAGACAGT GCTTGTTGGT 
GGGAAAGAGA CAAGCACGAG TGGCATTGAT GCCTTTAGTG GAAGAGTGTT ATACACCTGC 
ACTGCCAATG GATGCGACAA ACAACAAAAT GTCCACGATG CTTCAGATTT GTTGATAGTG 
AAACGACAAC AACAAGTTGT TCGTTCAATC ATCAACAGAA ATGGACACGA AAATTGGAAC 
TTCAGTGTTG GTGAAATCAA CCTGGATTTT TCTCAAGGAG CAAATCTACA TCTGTTAGAG 
GAATCATCTG AACATTCAAA TGAACAATCA TACCAGAAAC AGGACACCAA ACAAACCCAC 
ACTGAGAGTG ATGAATTCTC AATGACGGAT GCTCATTGTA TTAAAGTTGT CGTACCAGAT 
GGAATGGTCT GTCTTGTGAC GAGAAAAAAT GAGCATGTAG TGAAATGGAA GCATAAACTT 
GGTTCTCCTG TAAGTGCAGC ATGGTTATTA GTGGATGGAG AACTGAGAAA ACTTGATTTA 
TTTGATCCAA GTATGGTCCC TGCATTGGAT GAAAGCACAG CGGTGGCAAA GAAGGCACCT 
CAGATTAACA CAATTTATAT GGGATCATAT GACAACCATG TTTACATCCA AGCCTCACCT 
GCTGTACAGA AATCTATTGC TATCATCACT GCAAGTAATA GATACGGCAG TACAAATGCA 
AGACAAATGT CACCCAAGTT CGCCTACCGT CGTTGCCACG GTGATATGAC ACCAGTTGCC 
ATAGAAACCA ACAAGGATGA CCCAACAACA GAGTTAGTGG TAAAAGGAAA ACCTCCGCAA 
AAAGCAAACT GTGTTCATGG GTTGACGCTC TACAATGAGC AGTTTCCTAA TGATGGAGGA 
TATTATTTAC AAGATGACTC GAGTTATTTA TCGAGAATAC AAAGCTCGAA GAGGATGGGA 
AGGAATACTG TTGACTCATT AGAGAATGAT TCATATGACA AATACAACAT AGATATCGTA 
TATTCTTCAT TCATGCACTG GTGGAAGGAA CTTCTTATAT TAACTGTGTC GGTTTCAATT 
TTTGTGCAAT GTCTTATCAA CAAGTGCATG CAAGCCTTCA AGTCTCGATC TACAAATGAA 
ACAACGACAA GTTTATATGA ATCACCACCA GAACATCCCC CTCCTGTTTG TGAGGTGGAG 
AGAGAATACG AATCAAGATT CTTGACTGAT TTTGAACCTT TGGAATGCCT TGGAAAGGGA 
GGATTTGGAA TCGTATTCAA GGCAAGAAAT AAAATGGATG ATTGCACATA TGCTGTGAAA 
CGAATTCTTC TTCCTAACAA TGTAAGTTCT CGGGATAAAG TCTTGCGAGA AGTGCGAGCG 
CTTGCAAAGT TGGACCATCC TGCCATAGTG AGATATTTCA ACTCGTGGAA TGAAGAACCT 
CCAGCAGGGT GGCAACAAAA GAGAGATGCT CACCTTAAAG ATATGAGTGC TTGGAGCAGC 
ACTGCGTTTC CCAACACAAG TGAAAAACCA ATCAGCAAGA GGAATAAACA TTCTACTTCA 
AGTGGGACTG GTCAACAAAG CACAGAATTA TCATCAAGTA ACCACATACA ACATGATGAT 
GATGACGTCA TATTTGGGTG TGGTACAACT CAAGGATTTG TTGTTGATAA GACAACAGAG 
AAGACAAGTG ACAGTGGATT GTTTGATGAT CAACATAACG ATAGTTGGGG TAACAAGGAT 
GAAACATGGG GTGATGAACC CGTTGAAATG AGATCCAACA TAGGGGGAAA GTTTTTCCAA 
AGTGACAGCT CCCTCCCCTC AGTCTCTGGA AGTATCGTAC GAAATCGGAA CAATAATAGT 
TCACCCAGAG CTTCTGTTCC ATTTGGAAGA TACATGATGC AAGATTCGCT GAGTGTTGTG 
TTTGATGATC AAGTTTCAAA TGCAGAGAAT TTCAGAACGA ATAATAAGGT CAATGATAAT 
AAACCACCAA GCTATGAAAG TTTGTTTTCC AACGCTGCTG TAAGAAGACG CCCATCAGCA 
AAAGCAACAG ACACCAGCAC TTCCGAATTA AAGGCAGTGG TTTGTATGCA AGATGCGAGC 
ACAGGACACC AGGGAAGACT TGGTCAGCAT AATGATGAAG ACACCCACCC ACAAGACAAC 
ACAATATATC TTTACATTCA AATGCAACTT TGTAAGAAAG AAAGCTTGAG AGAATGGCTT 
GCTGCTAATG TGGAGAAAAG GGATTTCCAC TACTGCTTGA ATATCTTTCA ACAGGTTGTC 
AGTGCAGTTG CCTATGTACA CGACTCTGGG TTGATTCACC GTGATCTAAA ACCATCCAAT 
GTTCTCTTCT CACTTGATGG GACAATAAAA GTTGGTGACT TTGGGTTGGT AACACACGCT 
GGAGAGGGAG ATCCGTTTTT TAACGTGGGA GAACTACCAG AGGCGATACT TGGTAAAGCA 
GATGACAAAC ATACACAAAG AGTTGGCACG AGAATGTACA TGGCTCCTGA GCAAATGTCC 
AGCAGCACAT ACAGTGAAAA GATCGATATA TTTGCACTTG GTTTGATATT TTTTGAGCTC 
ATTCACTCGT TTGGAACACA AATGGAAAGA ATTCTTCATC TTTCTGATGC AAGAAAACTC 
AAGTTTCCCA TTCAATTTTT AAACAACTAC CCGAAAGAGT CCAAGCTGAC TCATCAAATG 
CTGAGTCACA AAGCAAGTGA CCGACCTTCA GCCAACGAAG TGAATGAACA TGAAGTATTC 
ACAGAAGTTT TGGACTTACA ACAATCAAGG TCCCGGCATC GAACGACGTC ATCCAGAAGT 
TCTGACTCCA CTTCAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC494391 ( XM_002128452.3 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_002128488.1 Ciona intestinalis eukaryotic translation initiation factor 2-alpha kinase 3 (LOC494391), mRNA.

Price & Availability↑

CloneID	OCi01751
Clone ID Related Accession (Same CDS sequence)	XM_002128452.4 , XM_002128452.3
Accession Version	XM_002128452.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-20
Organism	Ciona intestinalis(vase tunicate)
Product	eukaryotic translation initiation factor 2-alpha kinase 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020167.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 21, 2016 this sequence version replaced XM_002128452.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGTG GTGTTACTGG CATTGCTAAG TTTTTGACTT CTGTTGGTTT ATTTTCTATT 
GTAACAGCTG GTTCACCAAA CACAGAAAAT CAATCAAAAG AAAAACCTAA AGCTGTCACC 
CTCTCAAGTT ATTCTGACCC ATTGCTCATT GCAACTACAC TCGATGGTAA AATCTCAGCA 
TTGAACATTG CTGATGGTGG AAAATTGAAG TGGGTTGTGG ATTTGGGTCA TGGACCTTTG 
GTTTCATCAT CTCTTGCAAA ACTTGTTTAT AAAGAAGATG GAAAATATGT GCGATTGATT 
CCTTCATTAC ACGGGGGATT ATTTCGCTTT GATGGAGAAT CTGTCGAGGC TGTCCCATTT 
GATGCTGATC AATTATTAAG CTCATCCTTT CGGTTGGCTG ACGAGACAGT GCTTGTTGGT 
GGGAAAGAGA CAAGCACGAG TGGCATTGAT GCCTTTAGTG GAAGAGTGTT ATACACCTGC 
ACTGCCAATG GATGCGACAA ACAACAAAAT GTCCACGATG CTTCAGATTT GTTGATAGTG 
AAACGACAAC AACAAGTTGT TCGTTCAATC ATCAACAGAA ATGGACACGA AAATTGGAAC 
TTCAGTGTTG GTGAAATCAA CCTGGATTTT TCTCAAGGAG CAAATCTACA TCTGTTAGAG 
GAATCATCTG AACATTCAAA TGAACAATCA TACCAGAAAC AGGACACCAA ACAAACCCAC 
ACTGAGAGTG ATGAATTCTC AATGACGGAT GCTCATTGTA TTAAAGTTGT CGTACCAGAT 
GGAATGGTCT GTCTTGTGAC GAGAAAAAAT GAGCATGTAG TGAAATGGAA GCATAAACTT 
GGTTCTCCTG TAAGTGCAGC ATGGTTATTA GTGGATGGAG AACTGAGAAA ACTTGATTTA 
TTTGATCCAA GTATGGTCCC TGCATTGGAT GAAAGCACAG CGGTGGCAAA GAAGGCACCT 
CAGATTAACA CAATTTATAT GGGATCATAT GACAACCATG TTTACATCCA AGCCTCACCT 
GCTGTACAGA AATCTATTGC TATCATCACT GCAAGTAATA GATACGGCAG TACAAATGCA 
AGACAAATGT CACCCAAGTT CGCCTACCGT CGTTGCCACG GTGATATGAC ACCAGTTGCC 
ATAGAAACCA ACAAGGATGA CCCAACAACA GAGTTAGTGG TAAAAGGAAA ACCTCCGCAA 
AAAGCAAACT GTGTTCATGG GTTGACGCTC TACAATGAGC AGTTTCCTAA TGATGGAGGA 
TATTATTTAC AAGATGACTC GAGTTATTTA TCGAGAATAC AAAGCTCGAA GAGGATGGGA 
AGGAATACTG TTGACTCATT AGAGAATGAT TCATATGACA AATACAACAT AGATATCGTA 
TATTCTTCAT TCATGCACTG GTGGAAGGAA CTTCTTATAT TAACTGTGTC GGTTTCAATT 
TTTGTGCAAT GTCTTATCAA CAAGTGCATG CAAGCCTTCA AGTCTCGATC TACAAATGAA 
ACAACGACAA GTTTATATGA ATCACCACCA GAACATCCCC CTCCTGTTTG TGAGGTGGAG 
AGAGAATACG AATCAAGATT CTTGACTGAT TTTGAACCTT TGGAATGCCT TGGAAAGGGA 
GGATTTGGAA TCGTATTCAA GGCAAGAAAT AAAATGGATG ATTGCACATA TGCTGTGAAA 
CGAATTCTTC TTCCTAACAA TGTAAGTTCT CGGGATAAAG TCTTGCGAGA AGTGCGAGCG 
CTTGCAAAGT TGGACCATCC TGCCATAGTG AGATATTTCA ACTCGTGGAA TGAAGAACCT 
CCAGCAGGGT GGCAACAAAA GAGAGATGCT CACCTTAAAG ATATGAGTGC TTGGAGCAGC 
ACTGCGTTTC CCAACACAAG TGAAAAACCA ATCAGCAAGA GGAATAAACA TTCTACTTCA 
AGTGGGACTG GTCAACAAAG CACAGAATTA TCATCAAGTA ACCACATACA ACATGATGAT 
GATGACGTCA TATTTGGGTG TGGTACAACT CAAGGATTTG TTGTTGATAA GACAACAGAG 
AAGACAAGTG ACAGTGGATT GTTTGATGAT CAACATAACG ATAGTTGGGG TAACAAGGAT 
GAAACATGGG GTGATGAACC CGTTGAAATG AGATCCAACA TAGGGGGAAA GTTTTTCCAA 
AGTGACAGCT CCCTCCCCTC AGTCTCTGGA AGTATCGTAC GAAATCGGAA CAATAATAGT 
TCACCCAGAG CTTCTGTTCC ATTTGGAAGA TACATGATGC AAGATTCGCT GAGTGTTGTG 
TTTGATGATC AAGTTTCAAA TGCAGAGAAT TTCAGAACGA ATAATAAGGT CAATGATAAT 
AAACCACCAA GCTATGAAAG TTTGTTTTCC AACGCTGCTG TAAGAAGACG CCCATCAGCA 
AAAGCAACAG ACACCAGCAC TTCCGAATTA AAGGCAGTGG TTTGTATGCA AGATGCGAGC 
ACAGGACACC AGGGAAGACT TGGTCAGCAT AATGATGAAG ACACCCACCC ACAAGACAAC 
ACAATATATC TTTACATTCA AATGCAACTT TGTAAGAAAG AAAGCTTGAG AGAATGGCTT 
GCTGCTAATG TGGAGAAAAG GGATTTCCAC TACTGCTTGA ATATCTTTCA ACAGGTTGTC 
AGTGCAGTTG CCTATGTACA CGACTCTGGG TTGATTCACC GTGATCTAAA ACCATCCAAT 
GTTCTCTTCT CACTTGATGG GACAATAAAA GTTGGTGACT TTGGGTTGGT AACACACGCT 
GGAGAGGGAG ATCCGTTTTT TAACGTGGGA GAACTACCAG AGGCGATACT TGGTAAAGCA 
GATGACAAAC ATACACAAAG AGTTGGCACG AGAATGTACA TGGCTCCTGA GCAAATGTCC 
AGCAGCACAT ACAGTGAAAA GATCGATATA TTTGCACTTG GTTTGATATT TTTTGAGCTC 
ATTCACTCGT TTGGAACACA AATGGAAAGA ATTCTTCATC TTTCTGATGC AAGAAAACTC 
AAGTTTCCCA TTCAATTTTT AAACAACTAC CCGAAAGAGT CCAAGCTGAC TCATCAAATG 
CTGAGTCACA AAGCAAGTGA CCGACCTTCA GCCAACGAAG TGAATGAACA TGAAGTATTC 
ACAGAAGTTT TGGACTTACA ACAATCAAGG TCCCGGCATC GAACGACGTC ATCCAGAAGT 
TCTGACTCCA CTTCAAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002128488.1
CDS	694..3894
Translation	MFGGVTGIAKFLTSVGLFSIVTAGSPNTENQSKEKPKAVTLSSYSDPLLIATTLDGKISALNIADGGKLKWVVDLGHGPLVSSSLAKLVYKEDGKYVRLIPSLHGGLFRFDGESVEAVPFDADQLLSSSFRLADETVLVGGKETSTSGIDAFSGRVLYTCTANGCDKQQNVHDASDLLIVKRQQQVVRSIINRNGHENWNFSVGEINLDFSQGANLHLLEESSEHSNEQSYQKQDTKQTHTESDEFSMTDAHCIKVVVPDGMVCLVTRKNEHVVKWKHKLGSPVSAAWLLVDGELRKLDLFDPSMVPALDESTAVAKKAPQINTIYMGSYDNHVYIQASPAVQKSIAIITASNRYGSTNARQMSPKFAYRRCHGDMTPVAIETNKDDPTTELVVKGKPPQKANCVHGLTLYNEQFPNDGGYYLQDDSSYLSRIQSSKRMGRNTVDSLENDSYDKYNIDIVYSSFMHWWKELLILTVSVSIFVQCLINKCMQAFKSRSTNETTTSLYESPPEHPPPVCEVEREYESRFLTDFEPLECLGKGGFGIVFKARNKMDDCTYAVKRILLPNNVSSRDKVLREVRALAKLDHPAIVRYFNSWNEEPPAGWQQKRDAHLKDMSAWSSTAFPNTSEKPISKRNKHSTSSGTGQQSTELSSSNHIQHDDDDVIFGCGTTQGFVVDKTTEKTSDSGLFDDQHNDSWGNKDETWGDEPVEMRSNIGGKFFQSDSSLPSVSGSIVRNRNNNSSPRASVPFGRYMMQDSLSVVFDDQVSNAENFRTNNKVNDNKPPSYESLFSNAAVRRRPSAKATDTSTSELKAVVCMQDASTGHQGRLGQHNDEDTHPQDNTIYLYIQMQLCKKESLREWLAANVEKRDFHYCLNIFQQVVSAVAYVHDSGLIHRDLKPSNVLFSLDGTIKVGDFGLVTHAGEGDPFFNVGELPEAILGKADDKHTQRVGTRMYMAPEQMSSSTYSEKIDIFALGLIFFELIHSFGTQMERILHLSDARKLKFPIQFLNNYPKESKLTHQMLSHKASDRPSANEVNEHEVFTEVLDLQQSRSRHRTTSSRSSDSTSS

Target ORF information:

RefSeq Version	XM_002128452.3
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis eukaryotic translation initiation factor 2-alpha kinase 3 (LOC494391), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002128452.3

ORF Insert Sequence:

ATGTTTGGTG GTGTTACTGG CATTGCTAAG TTTTTGACTT CTGTTGGTTT ATTTTCTATT 
GTAACAGCTG GTTCACCAAA CACAGAAAAT CAATCAAAAG AAAAACCTAA AGCTGTCACC 
CTCTCAAGTT ATTCTGACCC ATTGCTCATT GCAACTACAC TCGATGGTAA AATCTCAGCA 
TTGAACATTG CTGATGGTGG AAAATTGAAG TGGGTTGTGG ATTTGGGTCA TGGACCTTTG 
GTTTCATCAT CTCTTGCAAA ACTTGTTTAT AAAGAAGATG GAAAATATGT GCGATTGATT 
CCTTCATTAC ACGGGGGATT ATTTCGCTTT GATGGAGAAT CTGTCGAGGC TGTCCCATTT 
GATGCTGATC AATTATTAAG CTCATCCTTT CGGTTGGCTG ACGAGACAGT GCTTGTTGGT 
GGGAAAGAGA CAAGCACGAG TGGCATTGAT GCCTTTAGTG GAAGAGTGTT ATACACCTGC 
ACTGCCAATG GATGCGACAA ACAACAAAAT GTCCACGATG CTTCAGATTT GTTGATAGTG 
AAACGACAAC AACAAGTTGT TCGTTCAATC ATCAACAGAA ATGGACACGA AAATTGGAAC 
TTCAGTGTTG GTGAAATCAA CCTGGATTTT TCTCAAGGAG CAAATCTACA TCTGTTAGAG 
GAATCATCTG AACATTCAAA TGAACAATCA TACCAGAAAC AGGACACCAA ACAAACCCAC 
ACTGAGAGTG ATGAATTCTC AATGACGGAT GCTCATTGTA TTAAAGTTGT CGTACCAGAT 
GGAATGGTCT GTCTTGTGAC GAGAAAAAAT GAGCATGTAG TGAAATGGAA GCATAAACTT 
GGTTCTCCTG TAAGTGCAGC ATGGTTATTA GTGGATGGAG AACTGAGAAA ACTTGATTTA 
TTTGATCCAA GTATGGTCCC TGCATTGGAT GAAAGCACAG CGGTGGCAAA GAAGGCACCT 
CAGATTAACA CAATTTATAT GGGATCATAT GACAACCATG TTTACATCCA AGCCTCACCT 
GCTGTACAGA AATCTATTGC TATCATCACT GCAAGTAATA GATACGGCAG TACAAATGCA 
AGACAAATGT CACCCAAGTT CGCCTACCGT CGTTGCCACG GTGATATGAC ACCAGTTGCC 
ATAGAAACCA ACAAGGATGA CCCAACAACA GAGTTAGTGG TAAAAGGAAA ACCTCCGCAA 
AAAGCAAACT GTGTTCATGG GTTGACGCTC TACAATGAGC AGTTTCCTAA TGATGGAGGA 
TATTATTTAC AAGATGACTC GAGTTATTTA TCGAGAATAC AAAGCTCGAA GAGGATGGGA 
AGGAATACTG TTGACTCATT AGAGAATGAT TCATATGACA AATACAACAT AGATATCGTA 
TATTCTTCAT TCATGCACTG GTGGAAGGAA CTTCTTATAT TAACTGTGTC GGTTTCAATT 
TTTGTGCAAT GTCTTATCAA CAAGTGCATG CAAGCCTTCA AGTCTCGATC TACAAATGAA 
ACAACGACAA GTTTATATGA ATCACCACCA GAACATCCCC CTCCTGTTTG TGAGGTGGAG 
AGAGAATACG AATCAAGATT CTTGACTGAT TTTGAACCTT TGGAATGCCT TGGAAAGGGA 
GGATTTGGAA TCGTATTCAA GGCAAGAAAT AAAATGGATG ATTGCACATA TGCTGTGAAA 
CGAATTCTTC TTCCTAACAA TGTAAGTTCT CGGGATAAAG TCTTGCGAGA AGTGCGAGCG 
CTTGCAAAGT TGGACCATCC TGCCATAGTG AGATATTTCA ACTCGTGGAA TGAAGAACCT 
CCAGCAGGGT GGCAACAAAA GAGAGATGCT CACCTTAAAG ATATGAGTGC TTGGAGCAGC 
ACTGCGTTTC CCAACACAAG TGAAAAACCA ATCAGCAAGA GGAATAAACA TTCTACTTCA 
AGTGGGACTG GTCAACAAAG CACAGAATTA TCATCAAGTA ACCACATACA ACATGATGAT 
GATGACGTCA TATTTGGGTG TGGTACAACT CAAGGATTTG TTGTTGATAA GACAACAGAG 
AAGACAAGTG ACAGTGGATT GTTTGATGAT CAACATAACG ATAGTTGGGG TAACAAGGAT 
GAAACATGGG GTGATGAACC CGTTGAAATG AGATCCAACA TAGGGGGAAA GTTTTTCCAA 
AGTGACAGCT CCCTCCCCTC AGTCTCTGGA AGTATCGTAC GAAATCGGAA CAATAATAGT 
TCACCCAGAG CTTCTGTTCC ATTTGGAAGA TACATGATGC AAGATTCGCT GAGTGTTGTG 
TTTGATGATC AAGTTTCAAA TGCAGAGAAT TTCAGAACGA ATAATAAGGT CAATGATAAT 
AAACCACCAA GCTATGAAAG TTTGTTTTCC AACGCTGCTG TAAGAAGACG CCCATCAGCA 
AAAGCAACAG ACACCAGCAC TTCCGAATTA AAGGCAGTGG TTTGTATGCA AGATGCGAGC 
ACAGGACACC AGGGAAGACT TGGTCAGCAT AATGATGAAG ACACCCACCC ACAAGACAAC 
ACAATATATC TTTACATTCA AATGCAACTT TGTAAGAAAG AAAGCTTGAG AGAATGGCTT 
GCTGCTAATG TGGAGAAAAG GGATTTCCAC TACTGCTTGA ATATCTTTCA ACAGGTTGTC 
AGTGCAGTTG CCTATGTACA CGACTCTGGG TTGATTCACC GTGATCTAAA ACCATCCAAT 
GTTCTCTTCT CACTTGATGG GACAATAAAA GTTGGTGACT TTGGGTTGGT AACACACGCT 
GGAGAGGGAG ATCCGTTTTT TAACGTGGGA GAACTACCAG AGGCGATACT TGGTAAAGCA 
GATGACAAAC ATACACAAAG AGTTGGCACG AGAATGTACA TGGCTCCTGA GCAAATGTCC 
AGCAGCACAT ACAGTGAAAA GATCGATATA TTTGCACTTG GTTTGATATT TTTTGAGCTC 
ATTCACTCGT TTGGAACACA AATGGAAAGA ATTCTTCATC TTTCTGATGC AAGAAAACTC 
AAGTTTCCCA TTCAATTTTT AAACAACTAC CCGAAAGAGT CCAAGCTGAC TCATCAAATG 
CTGAGTCACA AAGCAAGTGA CCGACCTTCA GCCAACGAAG TGAATGAACA TGAAGTATTC 
ACAGAAGTTT TGGACTTACA ACAATCAAGG TCCCGGCATC GAACGACGTC ATCCAGAAGT 
TCTGACTCCA CTTCAAGCTA A