chat cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	chat
Entrez Gene ID	445622
Full Name	choline acetyltransferase
Synonyms	Ci-ChAT
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001032449.1	NP_001027621.1	choline acetyltransferase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Developmental expression of ascidian neurotransmitter synthesis genes. I. Choline acetyltransferase and acetylcholine transporter genes.
Takamura K, Egawa T, Ohnishi S, Okada T, Fukuoka T
Development genes and evolution212(1)50-3(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCi13210	NM_001032449.1 Latest version!	Ciona intestinalis choline acetyltransferase (chat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chat ( NM_001032449.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> NP_001027621.1 Ciona intestinalis choline acetyltransferase (chat), mRNA.

Price & Availability↑

CloneID	OCi13210
Clone ID Related Accession (Same CDS sequence)	NM_001032449.1
Accession Version	NM_001032449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Ciona intestinalis(vase tunicate)
Product	choline acetyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB071999.1. ##Evidence-Data-START## Transcript exon combination :: AB071999.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028080, SAMD00028081 [ECO:0000350] ##Evidence-Data-END##

ATGCCTGGTG CACTACATCA GAAACCACTG GAAAAAAGAA GCAGCCGCTT AAATATACAG 
AAATTATCAT GTTCTACATC CTTGCCTTCC CTTCCACTGC CGGATCTTCA AGAAACACTA 
GAACAATATC TTCTGTGTAT AAAAGCGCTC GTGTCCGAAT CTCAATTTGA ACAAACCAAG 
GAAATTGTCG AATCGTTCGG TGCAACAGGT GGTGTAGGTG AAGCTATTCA AAGACGTCTT 
GAAGAATGTG CCAAAACTAA AGTCAACTGG GCATACGAGT GGTGGCTAAA TGACATGTAC 
TTAACAAATC CTGCCTCGTT ACCAATTAAT TCAAATCCTG GGATGGTTCT TCCAAAACAA 
AACTTTGAAG ATCAGCTGAG CATGCTCCAA TACGCTGCTA AGATTACTTC AGGAATTCTG 
GACTTCAAAG TTATATTAGA CGCACGTGCC CTACCCGTTG ATGTTGCAAG AGGACAGCTT 
GCCGGTCAAG CAATGTGTAT GGAACAATAC TACAGACTGC TTAATTCATA TAGACTCCCT 
GGATATACAC GTGATAAACT TGTTTCGCTA AAATCCGTTC AATCTGATGA ACCAGAACAC 
GTGATCGTTG TTTGTAACAA CCAGTTTTTC GTAGTTCCAG TCATGATGGG CAATTACCGA 
TTGAGTGAGG CCGAATTTGT CAGCCAATTC CAGCGTATTT TAGATGCCGC TCACGATCCT 
GCTGAACATA ATCCCCCGGT TGGAGTTTTA ACGTCACTGA ACCGACCAGA TTGGGCTGAG 
GCTCGGGAGA TTATGATGTC AGAACCAATA AATCGCCTCT CACTCGATTA CATCGAACAA 
AGTATTTTCA TTCTGTGTCT CGATAAATCA CTGGAAAATG AAGATGACGT CACAATGGCG 
CACCAAATGC TTTATGGAGG AGGACCAGAA AAAAACAGTT GTAACAGGTG GTACGACAAA 
ACTATCCAAC TTATAATCGG TTCTGACGGA AAAACAGGTC TTTCTTATGA ACATTCTGCT 
TCTGAGGGTA TAGCTGTCAC CGCGCTCATA GAACACTTGT TAAAATACAT AGCACTACAA 
GAGAAAAAAG GAGCATACAG ATCAAATTCA AGCATCGTGC TACCTTCCCC ACGACGCCTC 
CAGTGGAGAC TTAACCCTTC TGTTAATCAG TATGTCGAAA TTGGTAAAGC CAAAAACAGG 
CACGTTGCGA ATTCACTAGA TTTAGAAATT CTTCGATTCA CGAATTACGG CAAAACCTTC 
ATCAAACGAC AGAACATGAG CCCAGATGCT TACGTGCAAG TTTGTCTACA AATTACATAT 
TACAAGCTAT ACAAACGTTT GGTATCTACG TATGAAAGTG CTTCAACCAG AAGATTTAGA 
GAAGGCAGAG TGGATAACAT AAGAGCTGCA ACGGTTCAGG CGCTTGAATT AGCGAAAGGA 
TTGACTGAAC CTGATGAAAG CGGGAAGATA AAAATGGACG CAAAAAAGAT GTTACTGTTG 
CGGAGGGCTG TGAAAGCACA AACTGACTTC ACCATTGCAG CCATCACTGG TCATGGCATT 
GATTGTCATT TACTTGGACT TAAACAAATG GCAGACGAAA TGGGTCTTCC AACCCCAGGT 
ATATTCAAAG ACTCTTCATA TGAGCTTAGC AATTATTTTC AACTCTCTAC AAGTCAGATA 
CCAACGAGTA CAGATTCTTT TATGTGCTAT GGACCAGTGG TGCCGGACGG TTATGGTGTT 
TCCTACAATC CTCACAGCAA TTCAATCATT TTCTGTACAG CATCATTTCG TACTTGCCCG 
AGTACAAGTT CCGAAAAGTT TGTTGCAGCA TTGGTTGAAT CTCTACATAT AATGAAAGAA 
ATTTGTGTCC AGTGGAATAG ACGTAGATCG TCAAGTCTAG AGCACCTGTC GATTCATCGC 
AAACTCTCTT TACATCGTCG CGTAGATGCG AGATCGTATT CAGTCGATTG TGACGCATTA 
AGGGAGGAAT TCTCCGCGGA ATCAGATTCA AGTGATAATG AGGTTTTGGA GAAGAAGAAG 
ATACTAAAAA AGCAAAAAAG TTTTACCACC ACAAGAATGA TTAAGCGAAA ACTGGAACGG 
AGAGTTTCTT TGATATCCAA TTTAAATGCA CACCATCGTG GCAACAGCAA ATGTGGTATT 
GTTGATAAAA CACAAACTCA TACCTCGTCA AATGTAGAAA GTTCACAAAT TCCATGTGAC 
CTGATGAAGC TTCTGAGACA AGAAAGTTTA AATCAAAAAA ACAATATCAC CATTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027621.1
CDS	103..2382
Translation	MPGALHQKPLEKRSSRLNIQKLSCSTSLPSLPLPDLQETLEQYLLCIKALVSESQFEQTKEIVESFGATGGVGEAIQRRLEECAKTKVNWAYEWWLNDMYLTNPASLPINSNPGMVLPKQNFEDQLSMLQYAAKITSGILDFKVILDARALPVDVARGQLAGQAMCMEQYYRLLNSYRLPGYTRDKLVSLKSVQSDEPEHVIVVCNNQFFVVPVMMGNYRLSEAEFVSQFQRILDAAHDPAEHNPPVGVLTSLNRPDWAEAREIMMSEPINRLSLDYIEQSIFILCLDKSLENEDDVTMAHQMLYGGGPEKNSCNRWYDKTIQLIIGSDGKTGLSYEHSASEGIAVTALIEHLLKYIALQEKKGAYRSNSSIVLPSPRRLQWRLNPSVNQYVEIGKAKNRHVANSLDLEILRFTNYGKTFIKRQNMSPDAYVQVCLQITYYKLYKRLVSTYESASTRRFREGRVDNIRAATVQALELAKGLTEPDESGKIKMDAKKMLLLRRAVKAQTDFTIAAITGHGIDCHLLGLKQMADEMGLPTPGIFKDSSYELSNYFQLSTSQIPTSTDSFMCYGPVVPDGYGVSYNPHSNSIIFCTASFRTCPSTSSEKFVAALVESLHIMKEICVQWNRRRSSSLEHLSIHRKLSLHRRVDARSYSVDCDALREEFSAESDSSDNEVLEKKKILKKQKSFTTTRMIKRKLERRVSLISNLNAHHRGNSKCGIVDKTQTHTSSNVESSQIPCDLMKLLRQESLNQKNNITIS

Target ORF information:

RefSeq Version	NM_001032449.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis choline acetyltransferase (chat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032449.1

ORF Insert Sequence:

ATGCCTGGTG CACTACATCA GAAACCACTG GAAAAAAGAA GCAGCCGCTT AAATATACAG 
AAATTATCAT GTTCTACATC CTTGCCTTCC CTTCCACTGC CGGATCTTCA AGAAACACTA 
GAACAATATC TTCTGTGTAT AAAAGCGCTC GTGTCCGAAT CTCAATTTGA ACAAACCAAG 
GAAATTGTCG AATCGTTCGG TGCAACAGGT GGTGTAGGTG AAGCTATTCA AAGACGTCTT 
GAAGAATGTG CCAAAACTAA AGTCAACTGG GCATACGAGT GGTGGCTAAA TGACATGTAC 
TTAACAAATC CTGCCTCGTT ACCAATTAAT TCAAATCCTG GGATGGTTCT TCCAAAACAA 
AACTTTGAAG ATCAGCTGAG CATGCTCCAA TACGCTGCTA AGATTACTTC AGGAATTCTG 
GACTTCAAAG TTATATTAGA CGCACGTGCC CTACCCGTTG ATGTTGCAAG AGGACAGCTT 
GCCGGTCAAG CAATGTGTAT GGAACAATAC TACAGACTGC TTAATTCATA TAGACTCCCT 
GGATATACAC GTGATAAACT TGTTTCGCTA AAATCCGTTC AATCTGATGA ACCAGAACAC 
GTGATCGTTG TTTGTAACAA CCAGTTTTTC GTAGTTCCAG TCATGATGGG CAATTACCGA 
TTGAGTGAGG CCGAATTTGT CAGCCAATTC CAGCGTATTT TAGATGCCGC TCACGATCCT 
GCTGAACATA ATCCCCCGGT TGGAGTTTTA ACGTCACTGA ACCGACCAGA TTGGGCTGAG 
GCTCGGGAGA TTATGATGTC AGAACCAATA AATCGCCTCT CACTCGATTA CATCGAACAA 
AGTATTTTCA TTCTGTGTCT CGATAAATCA CTGGAAAATG AAGATGACGT CACAATGGCG 
CACCAAATGC TTTATGGAGG AGGACCAGAA AAAAACAGTT GTAACAGGTG GTACGACAAA 
ACTATCCAAC TTATAATCGG TTCTGACGGA AAAACAGGTC TTTCTTATGA ACATTCTGCT 
TCTGAGGGTA TAGCTGTCAC CGCGCTCATA GAACACTTGT TAAAATACAT AGCACTACAA 
GAGAAAAAAG GAGCATACAG ATCAAATTCA AGCATCGTGC TACCTTCCCC ACGACGCCTC 
CAGTGGAGAC TTAACCCTTC TGTTAATCAG TATGTCGAAA TTGGTAAAGC CAAAAACAGG 
CACGTTGCGA ATTCACTAGA TTTAGAAATT CTTCGATTCA CGAATTACGG CAAAACCTTC 
ATCAAACGAC AGAACATGAG CCCAGATGCT TACGTGCAAG TTTGTCTACA AATTACATAT 
TACAAGCTAT ACAAACGTTT GGTATCTACG TATGAAAGTG CTTCAACCAG AAGATTTAGA 
GAAGGCAGAG TGGATAACAT AAGAGCTGCA ACGGTTCAGG CGCTTGAATT AGCGAAAGGA 
TTGACTGAAC CTGATGAAAG CGGGAAGATA AAAATGGACG CAAAAAAGAT GTTACTGTTG 
CGGAGGGCTG TGAAAGCACA AACTGACTTC ACCATTGCAG CCATCACTGG TCATGGCATT 
GATTGTCATT TACTTGGACT TAAACAAATG GCAGACGAAA TGGGTCTTCC AACCCCAGGT 
ATATTCAAAG ACTCTTCATA TGAGCTTAGC AATTATTTTC AACTCTCTAC AAGTCAGATA 
CCAACGAGTA CAGATTCTTT TATGTGCTAT GGACCAGTGG TGCCGGACGG TTATGGTGTT 
TCCTACAATC CTCACAGCAA TTCAATCATT TTCTGTACAG CATCATTTCG TACTTGCCCG 
AGTACAAGTT CCGAAAAGTT TGTTGCAGCA TTGGTTGAAT CTCTACATAT AATGAAAGAA 
ATTTGTGTCC AGTGGAATAG ACGTAGATCG TCAAGTCTAG AGCACCTGTC GATTCATCGC 
AAACTCTCTT TACATCGTCG CGTAGATGCG AGATCGTATT CAGTCGATTG TGACGCATTA 
AGGGAGGAAT TCTCCGCGGA ATCAGATTCA AGTGATAATG AGGTTTTGGA GAAGAAGAAG 
ATACTAAAAA AGCAAAAAAG TTTTACCACC ACAAGAATGA TTAAGCGAAA ACTGGAACGG 
AGAGTTTCTT TGATATCCAA TTTAAATGCA CACCATCGTG GCAACAGCAA ATGTGGTATT 
GTTGATAAAA CACAAACTCA TACCTCGTCA AATGTAGAAA GTTCACAAAT TCCATGTGAC 
CTGATGAAGC TTCTGAGACA AGAAAGTTTA AATCAAAAAA ACAATATCAC CATTTCTTAA