LOC100179130 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100179130
Entrez Gene ID	100179130
Full Name	maltase-glucoamylase, intestinal-like
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
XM_026838510.1	XP_026694311.1	LOW QUALITY PROTEIN: lysosomal alpha-glucosidase-like
XM_018815570.1	XP_018671115.1	maltase-glucoamylase, intestinal-like

Pathways from BioSystems
Gene Ontology

The following LOC100179130 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100179130 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCi69565	XM_018815570.1 Latest version!	Ciona intestinalis maltase-glucoamylase, intestinal-like (LOC100179130), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCi152608	XM_026838510.1 Latest version!	Ciona intestinalis lysosomal alpha-glucosidase-like (LOC100179130), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100179130 ( XM_018815570.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018671115.1 Ciona intestinalis maltase-glucoamylase, intestinal-like (LOC100179130), mRNA.

Price & Availability↑

CloneID	OCi69565
Clone ID Related Accession (Same CDS sequence)	XM_018815570.1
Accession Version	XM_018815570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-20
Organism	Ciona intestinalis(vase tunicate)
Product	maltase-glucoamylase, intestinal-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004190416.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 100% of CDS bases ##RefSeq-Attributes-END##

ATGCATTTCT TATGGTGGTT AGTCCTTGCC ATTAGCTGTG AAGCTGCATT AGGCTACACA 
GCGGCCACTT GTCCATCCAC GGTCCCGGAA TGGAGCCGAG TTGATTGTTA CCCGGAGTCT 
GGATCCAACG AAGCGAGCTG TAATAGCAGA GGTTGTATGT GGTGTGAAGC GTCAGTTCAA 
GGCCCTCCGT GGTGTTTTTA CGATGATGCA ACGACTGCAG CGCCTCCAGG GGAAGGCTGC 
TCGTCCTGTA GTTCATGCAG CAACAAAGTA GATTGTTACC CTGAAGCTGG GTCCAGTGAA 
GGCGGTTGTT ACGACCGTGG TTGTCTATGG TGTCCATCTA ATGTTCCAAA TGAACCATGG 
TGCCTTGTTC CTGATGGAGG TGCAATTGTG AACCCACCAA TCCAGACACT GGATGTCCTT 
CTGACATCCC AGAAGCCGAG AGAATCGATT GTTATCCTGG TGGAGGAGCT TCTCAATCTG 
GATAGTGCTG GCTGCATTTG GTGCGAGAGC AATACTAATG GCGTACCATG GTGTTTTTTC 
AACACAACCA ATCTTCCAGA AGGTATTGAC GATGATGAAA GAGTCAATTG CATTCCTGAA 
GGTGGTTTCT CTCCATCACT TTGCACTGAA AGATCATGTG CTTTTGCAAA CACCAACTCT 
CCAAATGCCC CAATGTGCTA TTTTCAGAAT GATCAATATG GGTACTCAAT GCAAGGCACC 
CCTGTTGCAA CCTACAATGG ATATCGTGTA ACTCTTAACA GAATCCACAC TTCTACTTTA 
TTTGGTGATG ATGTAGACAC AGTGACGCTG GATGTTACTT TCGATACTCA GTCCAGAATC 
CACATTAAGT TTTACGATTC CTCTGAAGAT CGATTTGAGG TTCCTCTTAC AATCAATGGA 
GCAGACCCAA GCCCCCCTTC AAACCCACTT TATGATATAC AGTTTTTCAA TGATCCTTCT 
TTTTACTTTA AAGTGATCAG ACAATCCACC GGAGCTGTTC TACTCGATAC TTCATTAGGT 
GGTTTGACCT TCAGTAATCA GTTCCTGCAG ATCGCAACTC GTGTTCCAAC CAAAACAATG 
TATGGGTTCG GTGAACAAGA ACATCAAACG CTTGCTCACA CATTTGAATG GGAGAGCTTT 
GGAATGTACG CGAGGGACCA ACCCCCAGAT CCAGGTGCAA ATCTCTATGG GACTCACCCA 
TTCTATGTAT CTGTTGAAGA TGATGGAAAA TCTCACGGAG TTCTTTTCCT CAATTCCAAT 
GCACAAGATG TGACCTTGAC ACCAGCACCT GGCGTTGTGT ACAGAACTAT AGGAGGTGTA 
TTGGACATGT ATGTCTTCCT TGGTCCAGAA CCAAACAGTG TAATTGAACA GTATAACACT 
GCCATTGGAA CTCCATTCAT GCCCCCATAT TGGTCCCTTG GATTCCAGCT TTGTCGTTAT 
GGTTACGGAA GCTTGGATGT TGTGAAGGCC ACTGTGGCAA GAATGGATGC TTATGATATC 
CCAATGGACG TTCAGTATGG TGATATTGAC TACATGGATG AACGAAGAGA CTTCACTTAC 
GATCATACCA ACTATGCTGG CTTGCCTGAT TATGTTAAAC AGCTACAAAG CGGTGGAAAG 
CATTATATTA TTATCTTGGA TCCCTGCATC ACTGAAGACG ACCCAGCTGG TACTTACCCA 
CCTTATGACA TAGGAGCTTC GATGAATGTG TTTGTTACCG AGTCAGATGG AGTAACTCCT 
GCATCTGGAC GAGTGTGGCC TCCTGGTCAA TGTGCATTTC CAGATTACAC CAACCCAGAC 
ACTGAAACCT GGTGGACAGA TCAGTGTGTA GACTTCCATA AGACAATCAA CTTTGATGGA 
CTTTGGATTG ATATGAACGA ACCAGCAAAC TTTGTGGCCG GCAGCACGAG TGGTTGCGCA 
ACCAATGCTC TTAACAACCC GCCTTATAAA CCAAAGATCT GGGGTGATAT ACTTGCGGAT 
AAGACCATGT GTCCTGATCA TTTGCATAAA CTTGGTAACC ATTACGATGT GCATAACTTG 
TATGGGTGGA GTCAATCTAA TGTGACGATA CTATCTGCCA CAGCAGCAAC CGGTAAGCGA 
CCATTCGTAG TTAGCAGAAG TACGTACGTT GGGTCGGGAC AATGGGCGGC ACATTGGCTT 
GGTGATAACA ACTCTGATTG GCATGATCTG AAAATGTCAG TCATTGGAAT GTTGGAATTC 
AACATGTTTG GAATACCTTA TGTTGGAGCT GACATCTGTG GGTTCAATGG TGACGCTCAG 
GAAGAACTTT GCGATCGTTG GATGGAACTT GGTGCATTCT ACCCGTTCTC CAGAAACCAT 
AATGGGCTAG GATACAAAGA GCAAGACCCT GCCGCATGGG GGGATGCATT CGCTGCTCGA 
TCGAGGGCAG TACTTCGTAC TCGATATACT TTGCTACCAT ATTTGTACGA GCTCTTCTAC 
ACAGCACACA CTGCAGGTAC TGGTATTGTG CGACCTCTAT CTTTTGAGTT TATTAATGAC 
GTAAACACAA TTACTATCGA CGAGCAATTT ATGTGGGGTC CTGCACTTCT CTTTTCCCCT 
GTGTTGTACC AAGGAGAGAC CTCTGTTTCT GCTTACTTCC CTGATGCAAG ATGGTATTGT 
AGCCACCTTT TTATATTTTT ATTGACTTTT GCTACAGCAT TTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018671115.1
CDS	1..2688
Translation	MHFLWWLVLAISCEAALGYTAATCPSTVPEWSRVDCYPESGSNEASCNSRGCMWCEASVQGPPWCFYDDATTAAPPGEGCSSCSSCSNKVDCYPEAGSSEGGCYDRGCLWCPSNVPNEPWCLVPDGGAIVNPPIQTLDVLLTSQKPRESIVILVEELLNLDSAGCIWCESNTNGVPWCFFNTTNLPEGIDDDERVNCIPEGGFSPSLCTERSCAFANTNSPNAPMCYFQNDQYGYSMQGTPVATYNGYRVTLNRIHTSTLFGDDVDTVTLDVTFDTQSRIHIKFYDSSEDRFEVPLTINGADPSPPSNPLYDIQFFNDPSFYFKVIRQSTGAVLLDTSLGGLTFSNQFLQIATRVPTKTMYGFGEQEHQTLAHTFEWESFGMYARDQPPDPGANLYGTHPFYVSVEDDGKSHGVLFLNSNAQDVTLTPAPGVVYRTIGGVLDMYVFLGPEPNSVIEQYNTAIGTPFMPPYWSLGFQLCRYGYGSLDVVKATVARMDAYDIPMDVQYGDIDYMDERRDFTYDHTNYAGLPDYVKQLQSGGKHYIIILDPCITEDDPAGTYPPYDIGASMNVFVTESDGVTPASGRVWPPGQCAFPDYTNPDTETWWTDQCVDFHKTINFDGLWIDMNEPANFVAGSTSGCATNALNNPPYKPKIWGDILADKTMCPDHLHKLGNHYDVHNLYGWSQSNVTILSATAATGKRPFVVSRSTYVGSGQWAAHWLGDNNSDWHDLKMSVIGMLEFNMFGIPYVGADICGFNGDAQEELCDRWMELGAFYPFSRNHNGLGYKEQDPAAWGDAFAARSRAVLRTRYTLLPYLYELFYTAHTAGTGIVRPLSFEFINDVNTITIDEQFMWGPALLFSPVLYQGETSVSAYFPDARWYCSHLFIFLLTFATAFD

Target ORF information:

RefSeq Version	XM_018815570.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis maltase-glucoamylase, intestinal-like (LOC100179130), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018815570.1

ORF Insert Sequence:

ATGCATTTCT TATGGTGGTT AGTCCTTGCC ATTAGCTGTG AAGCTGCATT AGGCTACACA 
GCGGCCACTT GTCCATCCAC GGTCCCGGAA TGGAGCCGAG TTGATTGTTA CCCGGAGTCT 
GGATCCAACG AAGCGAGCTG TAATAGCAGA GGTTGTATGT GGTGTGAAGC GTCAGTTCAA 
GGCCCTCCGT GGTGTTTTTA CGATGATGCA ACGACTGCAG CGCCTCCAGG GGAAGGCTGC 
TCGTCCTGTA GTTCATGCAG CAACAAAGTA GATTGTTACC CTGAAGCTGG GTCCAGTGAA 
GGCGGTTGTT ACGACCGTGG TTGTCTATGG TGTCCATCTA ATGTTCCAAA TGAACCATGG 
TGCCTTGTTC CTGATGGAGG TGCAATTGTG AACCCACCAA TCCAGACACT GGATGTCCTT 
CTGACATCCC AGAAGCCGAG AGAATCGATT GTTATCCTGG TGGAGGAGCT TCTCAATCTG 
GATAGTGCTG GCTGCATTTG GTGCGAGAGC AATACTAATG GCGTACCATG GTGTTTTTTC 
AACACAACCA ATCTTCCAGA AGGTATTGAC GATGATGAAA GAGTCAATTG CATTCCTGAA 
GGTGGTTTCT CTCCATCACT TTGCACTGAA AGATCATGTG CTTTTGCAAA CACCAACTCT 
CCAAATGCCC CAATGTGCTA TTTTCAGAAT GATCAATATG GGTACTCAAT GCAAGGCACC 
CCTGTTGCAA CCTACAATGG ATATCGTGTA ACTCTTAACA GAATCCACAC TTCTACTTTA 
TTTGGTGATG ATGTAGACAC AGTGACGCTG GATGTTACTT TCGATACTCA GTCCAGAATC 
CACATTAAGT TTTACGATTC CTCTGAAGAT CGATTTGAGG TTCCTCTTAC AATCAATGGA 
GCAGACCCAA GCCCCCCTTC AAACCCACTT TATGATATAC AGTTTTTCAA TGATCCTTCT 
TTTTACTTTA AAGTGATCAG ACAATCCACC GGAGCTGTTC TACTCGATAC TTCATTAGGT 
GGTTTGACCT TCAGTAATCA GTTCCTGCAG ATCGCAACTC GTGTTCCAAC CAAAACAATG 
TATGGGTTCG GTGAACAAGA ACATCAAACG CTTGCTCACA CATTTGAATG GGAGAGCTTT 
GGAATGTACG CGAGGGACCA ACCCCCAGAT CCAGGTGCAA ATCTCTATGG GACTCACCCA 
TTCTATGTAT CTGTTGAAGA TGATGGAAAA TCTCACGGAG TTCTTTTCCT CAATTCCAAT 
GCACAAGATG TGACCTTGAC ACCAGCACCT GGCGTTGTGT ACAGAACTAT AGGAGGTGTA 
TTGGACATGT ATGTCTTCCT TGGTCCAGAA CCAAACAGTG TAATTGAACA GTATAACACT 
GCCATTGGAA CTCCATTCAT GCCCCCATAT TGGTCCCTTG GATTCCAGCT TTGTCGTTAT 
GGTTACGGAA GCTTGGATGT TGTGAAGGCC ACTGTGGCAA GAATGGATGC TTATGATATC 
CCAATGGACG TTCAGTATGG TGATATTGAC TACATGGATG AACGAAGAGA CTTCACTTAC 
GATCATACCA ACTATGCTGG CTTGCCTGAT TATGTTAAAC AGCTACAAAG CGGTGGAAAG 
CATTATATTA TTATCTTGGA TCCCTGCATC ACTGAAGACG ACCCAGCTGG TACTTACCCA 
CCTTATGACA TAGGAGCTTC GATGAATGTG TTTGTTACCG AGTCAGATGG AGTAACTCCT 
GCATCTGGAC GAGTGTGGCC TCCTGGTCAA TGTGCATTTC CAGATTACAC CAACCCAGAC 
ACTGAAACCT GGTGGACAGA TCAGTGTGTA GACTTCCATA AGACAATCAA CTTTGATGGA 
CTTTGGATTG ATATGAACGA ACCAGCAAAC TTTGTGGCCG GCAGCACGAG TGGTTGCGCA 
ACCAATGCTC TTAACAACCC GCCTTATAAA CCAAAGATCT GGGGTGATAT ACTTGCGGAT 
AAGACCATGT GTCCTGATCA TTTGCATAAA CTTGGTAACC ATTACGATGT GCATAACTTG 
TATGGGTGGA GTCAATCTAA TGTGACGATA CTATCTGCCA CAGCAGCAAC CGGTAAGCGA 
CCATTCGTAG TTAGCAGAAG TACGTACGTT GGGTCGGGAC AATGGGCGGC ACATTGGCTT 
GGTGATAACA ACTCTGATTG GCATGATCTG AAAATGTCAG TCATTGGAAT GTTGGAATTC 
AACATGTTTG GAATACCTTA TGTTGGAGCT GACATCTGTG GGTTCAATGG TGACGCTCAG 
GAAGAACTTT GCGATCGTTG GATGGAACTT GGTGCATTCT ACCCGTTCTC CAGAAACCAT 
AATGGGCTAG GATACAAAGA GCAAGACCCT GCCGCATGGG GGGATGCATT CGCTGCTCGA 
TCGAGGGCAG TACTTCGTAC TCGATATACT TTGCTACCAT ATTTGTACGA GCTCTTCTAC 
ACAGCACACA CTGCAGGTAC TGGTATTGTG CGACCTCTAT CTTTTGAGTT TATTAATGAC 
GTAAACACAA TTACTATCGA CGAGCAATTT ATGTGGGGTC CTGCACTTCT CTTTTCCCCT 
GTGTTGTACC AAGGAGAGAC CTCTGTTTCT GCTTACTTCC CTGATGCAAG ATGGTATTGT 
AGCCACCTTT TTATATTTTT ATTGACTTTT GCTACAGCAT TTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100179130 ( XM_026838510.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_026694311.1 Ciona intestinalis lysosomal alpha-glucosidase-like (LOC100179130), mRNA.

Price & Availability↑

CloneID	OCi152608
Clone ID Related Accession (Same CDS sequence)	XM_026838510.1
Accession Version	XM_026838510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Ciona intestinalis(vase tunicate)
Product	LOW QUALITY PROTEIN: lysosomal alpha-glucosidase-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004190416.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCATTTCT TATGGTGGTT AGTCCTTGCC ATTAGCTGTG AAGCTGCATT AGGCTACACA 
GCGGCCACTT GTCCATCCAC GGTCCCGGAA TGGAGCCGAG TTGATTGTTA CCCGGAGTCT 
GGATCCAACG AAGCGAGCTG TAATAGCAGA GGTTGTATGT GGTGTGAAGC GTCAGTTCAA 
GGCCCTCCGT GGTGTTTTTA CGATGATGCA ACGACTGCAG CGCCTCCAGG GGAAGGCTGC 
TCGTCCTGTA GTTCATGCAG CAACAAAGTA GATTGTTACC CTGAAGCTGG GTCCAGTGAA 
GGCGGTTGTT ACGACCGTGG TTGTCTATGG TGTCCATCTA ATGTTCCAAA TGAACCATGG 
TGCCTTGTTC CTGATGGAGG TGCAATTGTG AACCCACCAA TCCAGACACT GGATGTCCTT 
CTGACATCCC AGAAGCCGAG AGAATCGATT GTTATCCTGG TGGAGGAGCT TCTCAATCTG 
GATGTGAGAG TGCTGGCTGC ATTTGGTGCG AGAGCAATAC TAATGGCGTA CCATGGTGTT 
TTTTCAACAC AACCAATCTT CCAGAAGGTA TTGACGATGA TGAAAGAGTC AATTGCATTC 
CTGAAGGGTT TCTCTCCATC ACTTTGCACT GAAAGATCAT GTGCTTTTGC AAACACCAAC 
TCTCCAAATG CCCCAATGTG CTATTTTCAG AATGATCAAT ATGGGTACTC AATGCAAGGC 
ACCCCTGTTG CAACCTACAA TGGATATCGT GTAACTCTTA ACAGAATCCA CACTTCTACT 
TTATTTGGTG ATGATGTAGA CACAGTGACG CTGGATGTTA CTTTCGATAC TCAGTCCAGA 
ATCCACATTA AGTTTTACGA TTCCTCTGAA GATCGATTTG AGGTTCCTCT TACAATCAAT 
GGAGCAGACC CAAGCCCCCC TTCAAACCCA CTTTATGATA TACAGTTTTT CAATGATCCT 
TCTTTTTACT TTAAAGTGAT CAGACAATCC ACCGGAGCTG TTCTACTCGA TACTTCATTA 
GGTGGTTTGA CCTTCAGTAA TCAGTTCCTG CAGATCGCAA CTCGTGTTCC AACCAAAACA 
ATGTATGGGT TCGGTGAACA AGAACATCAA ACGCTTGCTC ACACATTTGA ATGGGAGAGC 
TTTGGAATGT ACGCGAGGGA CCAACCCCCA GATCCAGGTG CAAATCTCTA TGGGACTCAC 
CCATTCTATG TATCTGTTGA AGATGATGGA AAATCTCACG GAGTTCTTTT CCTCAATTCC 
AATGCACAAG ATGTGACCTT GACACCAGCA CCTGGCGTTG TGTACAGAAC TATAGGAGGT 
GTATTGGACA TGTATGTCTT CCTTGGTCCA GAACCAAACA GTGTAATTGA ACAGTATAAC 
ACTGCCATTG GAACTCCATT CATGCCCCCA TATTGGTCCC TTGGATTCCA GCTTTGTCGT 
TATGGTTACG GAAGCTTGGA TGTTGTGAAG GCCACTGTGG CAAGAATGGA TGCTTATGAT 
ATCCCAATGG ACGTTCAGTA TGGTGATATT GACTACATGG ATGAACGAAG AGACTTCACT 
TACGATCATA CCAACTATGC TGGCTTGCCT GATTATGTTA AACAGCTACA AAGCGGTGGA 
AAGCATTATA TTATTATCTT GGATCCCTGC ATCACTGAAG ACGACCCAGC TGGTACTTAC 
CCACCTTATG ACATAGGAGC TTCGATGAAT GTGTTTGTTA CCGAGTCAGA TGGAGTAACT 
CCTGCATCTG GACGAGTGTG GCCTCCTGGT CAATGTGCAT TTCCAGATTA CACCAACCCA 
GACACTGAAA CCTGGTGGAC AGATCAGTGT GTAGACTTCC ATAAGACAAT CAACTTTGAT 
GGACTTTGGA TTGATATGAA CGAACCAGCA AACTTTGTGG CCGGCAGCAC GAGTGGTTGC 
GCAACCAATG CTCTTAACAA CCCGCCTTAT AAACCAAAGA TCTGGGGTGA TATACTTGCG 
GATAAGACCA TGTGTCCTGA TCATTTGCAT AAACTTGGTA ACCATTACGA TGTGCATAAC 
TTGTATGGGT GGAGTCAATC TAATGTGACG ATACTATCTG CCACAGCAGC AACCGGTAAG 
CGACCATTCG TAGTTAGCAG AAGTACGTAC GTTGGGTCGG GACAATGGGC GGCACATTGG 
CTTGGTGATA ACAACTCTGA TTGGCATGAT CTGAAAATGT CAGTCATTGG AATGTTGGAA 
TTCAACATGT TTGGAATACC TTATGTTGGA GCTGACATCT GTGGGTTCAA TGGTGACGCT 
CAGGAAGAAC TTTGCGATCG TTGGATGGAA CTTGGTGCAT TCTACCCGTT CTCCAGAAAC 
CATAATGGGC TAGGATACAA AGAGCAAGAC CCTGCCGCAT GGGGGGATGC ATTCGCTGCT 
CGATCGAGGG CAGTACTTCG TACTCGATAT ACTTTGCTAC CATATTTGTA CGAGCTCTTC 
TACACAGCAC ACACTGCAGG TACTGGTATT GTGCGACCTC TATCTTTTGA GTTTATTAAT 
GACGTAAACA CAATTACTAT CGACGAGCAA TTTATGTGGG GTCCTGCACT TCTCTTTTCC 
CCTGTGTTGT ACCAAGGAGA GACCTCTGTT TCTGCTTACT TCCCTGATGC AAGATGGTAC 
GACTATGCAA ATGGTGTTGA GTTAGGTACA CGAGGTAATG TCGCCAGCAT TAGCGCTCCA 
ATAGGAACTA TTCCCATCCA TGTACGAGGT GGTTATGTCA TCCCAACCCA GGAACCTGCC 
TCTAACACTG AGTTATCAAG AAAAAATCCG TTCGGATTAA TTATTGCTTT GGATGACAGC 
GGTTCAGCTG TTGGGCAAAT GTACTGGGAT CAGGGAGATA CCTTGAACCC AATACTTAGC 
GGACAACATG CACTGTTTAC CTTCTCAGCA CAAAACAACA AACTGACCAC ATCTGCAAGT 
GGTAGCTCGA GCATAATGTC AAGCATGACT ATGGGTAGCT TGCGTATCAT GGGTGTATCA 
TCTTCCCCAC AATCTGTTAC TGTCAATGGA GTTTCCAAGT CATTCACGGT GTCTAACTCG 
CAAGTAACAG TTAGCAACAT AAACGTGGTG ATGAGCGGAT CATTTGAAGT ACAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026694311.1
CDS	153..3272
Translation	MHFLWWLVLAISCEAALGYTAATCPSTVPEWSRVDCYPESGSNEASCNSRGCMWCEASVQGPPWCFYDDATTAAPPGEGCSSCSSCSNKVDCYPEAGSSEGGCYDRGCLWCPSNVPNEPWCLVPDGGAIVNPPIQTLDVLLTSQKPRESIVILVEELLNLDVRVLAAFGARAILMAYHGVFSTQPIFQKVLTMMKESIAFLKGFSPSLCTERSCAFANTNSPNAPMCYFQNDQYGYSMQGTPVATYNGYRVTLNRIHTSTLFGDDVDTVTLDVTFDTQSRIHIKFYDSSEDRFEVPLTINGADPSPPSNPLYDIQFFNDPSFYFKVIRQSTGAVLLDTSLGGLTFSNQFLQIATRVPTKTMYGFGEQEHQTLAHTFEWESFGMYARDQPPDPGANLYGTHPFYVSVEDDGKSHGVLFLNSNAQDVTLTPAPGVVYRTIGGVLDMYVFLGPEPNSVIEQYNTAIGTPFMPPYWSLGFQLCRYGYGSLDVVKATVARMDAYDIPMDVQYGDIDYMDERRDFTYDHTNYAGLPDYVKQLQSGGKHYIIILDPCITEDDPAGTYPPYDIGASMNVFVTESDGVTPASGRVWPPGQCAFPDYTNPDTETWWTDQCVDFHKTINFDGLWIDMNEPANFVAGSTSGCATNALNNPPYKPKIWGDILADKTMCPDHLHKLGNHYDVHNLYGWSQSNVTILSATAATGKRPFVVSRSTYVGSGQWAAHWLGDNNSDWHDLKMSVIGMLEFNMFGIPYVGADICGFNGDAQEELCDRWMELGAFYPFSRNHNGLGYKEQDPAAWGDAFAARSRAVLRTRYTLLPYLYELFYTAHTAGTGIVRPLSFEFINDVNTITIDEQFMWGPALLFSPVLYQGETSVSAYFPDARWYDYANGVELGTRGNVASISAPIGTIPIHVRGGYVIPTQEPASNTELSRKNPFGLIIALDDSGSAVGQMYWDQGDTLNPILSGQHALFTFSAQNNKLTTSASGSSSIMSSMTMGSLRIMGVSSSPQSVTVNGVSKSFTVSNSQVTVSNINVVMSGSFEVQW

Target ORF information:

RefSeq Version	XM_026838510.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis lysosomal alpha-glucosidase-like (LOC100179130), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026838510.1

ORF Insert Sequence:

ATGCATTTCT TATGGTGGTT AGTCCTTGCC ATTAGCTGTG AAGCTGCATT AGGCTACACA 
GCGGCCACTT GTCCATCCAC GGTCCCGGAA TGGAGCCGAG TTGATTGTTA CCCGGAGTCT 
GGATCCAACG AAGCGAGCTG TAATAGCAGA GGTTGTATGT GGTGTGAAGC GTCAGTTCAA 
GGCCCTCCGT GGTGTTTTTA CGATGATGCA ACGACTGCAG CGCCTCCAGG GGAAGGCTGC 
TCGTCCTGTA GTTCATGCAG CAACAAAGTA GATTGTTACC CTGAAGCTGG GTCCAGTGAA 
GGCGGTTGTT ACGACCGTGG TTGTCTATGG TGTCCATCTA ATGTTCCAAA TGAACCATGG 
TGCCTTGTTC CTGATGGAGG TGCAATTGTG AACCCACCAA TCCAGACACT GGATGTCCTT 
CTGACATCCC AGAAGCCGAG AGAATCGATT GTTATCCTGG TGGAGGAGCT TCTCAATCTG 
GATGTGAGAG TGCTGGCTGC ATTTGGTGCG AGAGCAATAC TAATGGCGTA CCATGGTGTT 
TTTTCAACAC AACCAATCTT CCAGAAGGTA TTGACGATGA TGAAAGAGTC AATTGCATTC 
CTGAAGGGTT TCTCTCCATC ACTTTGCACT GAAAGATCAT GTGCTTTTGC AAACACCAAC 
TCTCCAAATG CCCCAATGTG CTATTTTCAG AATGATCAAT ATGGGTACTC AATGCAAGGC 
ACCCCTGTTG CAACCTACAA TGGATATCGT GTAACTCTTA ACAGAATCCA CACTTCTACT 
TTATTTGGTG ATGATGTAGA CACAGTGACG CTGGATGTTA CTTTCGATAC TCAGTCCAGA 
ATCCACATTA AGTTTTACGA TTCCTCTGAA GATCGATTTG AGGTTCCTCT TACAATCAAT 
GGAGCAGACC CAAGCCCCCC TTCAAACCCA CTTTATGATA TACAGTTTTT CAATGATCCT 
TCTTTTTACT TTAAAGTGAT CAGACAATCC ACCGGAGCTG TTCTACTCGA TACTTCATTA 
GGTGGTTTGA CCTTCAGTAA TCAGTTCCTG CAGATCGCAA CTCGTGTTCC AACCAAAACA 
ATGTATGGGT TCGGTGAACA AGAACATCAA ACGCTTGCTC ACACATTTGA ATGGGAGAGC 
TTTGGAATGT ACGCGAGGGA CCAACCCCCA GATCCAGGTG CAAATCTCTA TGGGACTCAC 
CCATTCTATG TATCTGTTGA AGATGATGGA AAATCTCACG GAGTTCTTTT CCTCAATTCC 
AATGCACAAG ATGTGACCTT GACACCAGCA CCTGGCGTTG TGTACAGAAC TATAGGAGGT 
GTATTGGACA TGTATGTCTT CCTTGGTCCA GAACCAAACA GTGTAATTGA ACAGTATAAC 
ACTGCCATTG GAACTCCATT CATGCCCCCA TATTGGTCCC TTGGATTCCA GCTTTGTCGT 
TATGGTTACG GAAGCTTGGA TGTTGTGAAG GCCACTGTGG CAAGAATGGA TGCTTATGAT 
ATCCCAATGG ACGTTCAGTA TGGTGATATT GACTACATGG ATGAACGAAG AGACTTCACT 
TACGATCATA CCAACTATGC TGGCTTGCCT GATTATGTTA AACAGCTACA AAGCGGTGGA 
AAGCATTATA TTATTATCTT GGATCCCTGC ATCACTGAAG ACGACCCAGC TGGTACTTAC 
CCACCTTATG ACATAGGAGC TTCGATGAAT GTGTTTGTTA CCGAGTCAGA TGGAGTAACT 
CCTGCATCTG GACGAGTGTG GCCTCCTGGT CAATGTGCAT TTCCAGATTA CACCAACCCA 
GACACTGAAA CCTGGTGGAC AGATCAGTGT GTAGACTTCC ATAAGACAAT CAACTTTGAT 
GGACTTTGGA TTGATATGAA CGAACCAGCA AACTTTGTGG CCGGCAGCAC GAGTGGTTGC 
GCAACCAATG CTCTTAACAA CCCGCCTTAT AAACCAAAGA TCTGGGGTGA TATACTTGCG 
GATAAGACCA TGTGTCCTGA TCATTTGCAT AAACTTGGTA ACCATTACGA TGTGCATAAC 
TTGTATGGGT GGAGTCAATC TAATGTGACG ATACTATCTG CCACAGCAGC AACCGGTAAG 
CGACCATTCG TAGTTAGCAG AAGTACGTAC GTTGGGTCGG GACAATGGGC GGCACATTGG 
CTTGGTGATA ACAACTCTGA TTGGCATGAT CTGAAAATGT CAGTCATTGG AATGTTGGAA 
TTCAACATGT TTGGAATACC TTATGTTGGA GCTGACATCT GTGGGTTCAA TGGTGACGCT 
CAGGAAGAAC TTTGCGATCG TTGGATGGAA CTTGGTGCAT TCTACCCGTT CTCCAGAAAC 
CATAATGGGC TAGGATACAA AGAGCAAGAC CCTGCCGCAT GGGGGGATGC ATTCGCTGCT 
CGATCGAGGG CAGTACTTCG TACTCGATAT ACTTTGCTAC CATATTTGTA CGAGCTCTTC 
TACACAGCAC ACACTGCAGG TACTGGTATT GTGCGACCTC TATCTTTTGA GTTTATTAAT 
GACGTAAACA CAATTACTAT CGACGAGCAA TTTATGTGGG GTCCTGCACT TCTCTTTTCC 
CCTGTGTTGT ACCAAGGAGA GACCTCTGTT TCTGCTTACT TCCCTGATGC AAGATGGTAC 
GACTATGCAA ATGGTGTTGA GTTAGGTACA CGAGGTAATG TCGCCAGCAT TAGCGCTCCA 
ATAGGAACTA TTCCCATCCA TGTACGAGGT GGTTATGTCA TCCCAACCCA GGAACCTGCC 
TCTAACACTG AGTTATCAAG AAAAAATCCG TTCGGATTAA TTATTGCTTT GGATGACAGC 
GGTTCAGCTG TTGGGCAAAT GTACTGGGAT CAGGGAGATA CCTTGAACCC AATACTTAGC 
GGACAACATG CACTGTTTAC CTTCTCAGCA CAAAACAACA AACTGACCAC ATCTGCAAGT 
GGTAGCTCGA GCATAATGTC AAGCATGACT ATGGGTAGCT TGCGTATCAT GGGTGTATCA 
TCTTCCCCAC AATCTGTTAC TGTCAATGGA GTTTCCAAGT CATTCACGGT GTCTAACTCG 
CAAGTAACAG TTAGCAACAT AAACGTGGTG ATGAGCGGAT CATTTGAAGT ACAGTGGTGA