ISA3 cDNA ORF clone, Chlamydomonas reinhardtii

The following ISA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ISA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCh06411 XM_001699402.1
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Chlamydomonas reinhardtii isoamylase, starch debranching enzyme (ISA3), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCh06411
    Clone ID Related Accession (Same CDS sequence) XM_001699402.1
    Accession Version XM_001699402.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-08-28
    Organism Chlamydomonas reinhardtii
    Product isoamylase, starch debranching enzyme
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001843820).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCAGCTGC GACAGGCGGG CCTGAGGGCT CGCAGCACCG CTTCGGGTGC AGTGCCATGC 
    ATTCCAGTTG GTCTTCACGC TGGCGCAATT GGCATGCAGT CGGCGCTGGC TGGCGGGCGC
    GCTGCGGGGC GAGGGCGTGT TGCAACACGA CTACCGGCAC CGAAGCGCCA GTCTGAGCGC
    GTACGCGTGG TGGCTCAGAG CGTTTCGACT CTTTCTACTT CACAGCAGCT CTATAGCTCA
    GTGGCTGTCG GCAAGCCCGA GCCTCTGGGC CCCAGCCGCT CCGGCTCCGG CGTCAACTTC
    GCGCTGTTCT CCAAACACGC CAGGAGCGTG AGGCTGTGCC TGTTTGACGG CGAGGCGCAG
    CCGCTGGGCG AGGTGGACTG CAACCGCACG GGCGACGTGT GGCACGTGGA GCTGGCGGAC
    CTGCCGCTCA GCGGGCTGCG CTACGGCTTC AAGGTGGCGG GCGACGGCGG CTGGGACACG
    GGCGCGCGCT GGGCGCCCGG CCGCGTGCTG CTGGACCCCT ACGCGCCGCT GGTGGCGGGG
    CGGCGCCAGT TCGGGGTGCG CGACGCCGTG GAGCAGTTCA AGGGCAAGGA GGGCAGCCAG
    TTTCTGGGCG CGTTCGACTT CTCCTCCGCG CCCTACGACT GGGGCGCTCA GGAGGAGGCG
    GGCCGCCCGC GCACCGCCCT CAAGGACCTG GTGATCTACG AGGTGCCGGT GCGCTGCTTC
    ACCGCCGACG GCTCCTCGGG CCTGCCGGCG GAGGAGCGCG GCACCTTCCG GGGCATGGCC
    GCCAAGGCCT CCTACCTGGC CGGCCTGGGC GTGAACGCGG TGGAGCTGCT GCCGGTGTTC
    GAGTGGGACG AGCTCGAGTT CCGCCGCACC CGCAACCCGC GCGACCACAT GACCAACGTC
    CTGCACGCGC AGGGCATCCA GGTGCTGCTG GACGTGGTGT ACAACCACAC GGCCGAGGCG
    GACGACAAGG ACCCCTACAC GCTGTGCATG CGCGGCGTGG ACAACAAGAC CTACTACATG
    ATCGACCCCA GCCAGTACGT GCAGCTGGTC AACTGGAGCG GGTGCGGCAA CACCGTCAAC
    GCCAACAACC CGCCCGTCAC GCAGATGATC ATCGACAGCC TGGTGCACTG GGTGACGGAG
    TACCACGTGG ACGGCTTCCG CTTCGACCTG GCCAGCTGCC TGTGCAGGGA CGAGCGCGGC
    CACCCCATGG CCGTGCCGCC GCTCATCCGC GCCATCAGCA AGCACCCGCT GCTGAGCCAG
    GTGCACTTGA TCGCCGAGCC CTGGGACATC GGCATGTACC AGGTGGGCAG CTTCCCCAAC
    TGGGACATCT GGGCGGAGTG GAACGGCAAG TACCGCGACG ACGTGCGCCG CTTCATCAAG
    GGAGACGCAG GCATGAAGCG CGCCTTCGCC ACGCGCCTGG CCGGCTCCGC CGACCTGTAC
    CAGACCAACA ACCGCAAGCC CTACCACTCC ATCAACTTCA TCACCGCGCA CGACGGCTTC
    AGCCTGTACG ACATGGTGTC CTACAACGAC AAACACAACG ACGCCAACGG CGAGGGCAAC
    CGCGACGGCA CCAACGACAA CTTCTCCTGG AACTGCGGCG CCGAGGGCGA GACCGGCAAC
    GGCGGCGTGC GGGCGCTGCG CCAGCGGCAG ATGCGCAACT GCATGGTGGC GCTCATGATG
    AGCCAGGGCA CACCCATGAT TGTCAGCGGC GATGAGATCA TCAAGACGCA CGGCGGCAAC
    AACAACTGGT ACGGCCACGA CACAGCCATG GCGCACCTGC AGTGGCCCGA GGGCGACGCC
    GACAAGGAGG CGCTGCTGCG CTTCTGCTCC CAGCTCATCG CCTTCCGCAA GAGCCACCCC
    GCGCTGGGGC GGGAACACTT CCTGGGCCCC AACGACATCA CCTGGCACGA GGACAACTGG
    GGCAACGACG AGAGCCGCTT CCTGGCCTTC ACGCTGCACC ACCGCGAGGC TGGCGACATC
    TACGCCGCCT TCAACGCCCA CGCCTTCGCC GTGTCCGCGC CGCTGCCGCA CCCGCCCGCC
    GGCCGCAAGT GGTGCCGCCT GGTGGACACC AACCTGCCGC CGCCCAAGGA CTTCACGCCC
    GGCGGCAACG CGGGTGTGGA TGCGGTGTAC GGCGTGCAGG CGTTCTCGTC AATTGTGCTT
    ATCGCCAAGC CGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001699454.1
    CDS172..2349
    Translation

    Target ORF information:

    RefSeq Version XM_001699402.1
    Organism Chlamydomonas reinhardtii
    Definition Chlamydomonas reinhardtii isoamylase, starch debranching enzyme (ISA3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001699402.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCAGCTGC GACAGGCGGG CCTGAGGGCT CGCAGCACCG CTTCGGGTGC AGTGCCATGC 
    ATTCCAGTTG GTCTTCACGC TGGCGCAATT GGCATGCAGT CGGCGCTGGC TGGCGGGCGC
    GCTGCGGGGC GAGGGCGTGT TGCAACACGA CTACCGGCAC CGAAGCGCCA GTCTGAGCGC
    GTACGCGTGG TGGCTCAGAG CGTTTCGACT CTTTCTACTT CACAGCAGCT CTATAGCTCA
    GTGGCTGTCG GCAAGCCCGA GCCTCTGGGC CCCAGCCGCT CCGGCTCCGG CGTCAACTTC
    GCGCTGTTCT CCAAACACGC CAGGAGCGTG AGGCTGTGCC TGTTTGACGG CGAGGCGCAG
    CCGCTGGGCG AGGTGGACTG CAACCGCACG GGCGACGTGT GGCACGTGGA GCTGGCGGAC
    CTGCCGCTCA GCGGGCTGCG CTACGGCTTC AAGGTGGCGG GCGACGGCGG CTGGGACACG
    GGCGCGCGCT GGGCGCCCGG CCGCGTGCTG CTGGACCCCT ACGCGCCGCT GGTGGCGGGG
    CGGCGCCAGT TCGGGGTGCG CGACGCCGTG GAGCAGTTCA AGGGCAAGGA GGGCAGCCAG
    TTTCTGGGCG CGTTCGACTT CTCCTCCGCG CCCTACGACT GGGGCGCTCA GGAGGAGGCG
    GGCCGCCCGC GCACCGCCCT CAAGGACCTG GTGATCTACG AGGTGCCGGT GCGCTGCTTC
    ACCGCCGACG GCTCCTCGGG CCTGCCGGCG GAGGAGCGCG GCACCTTCCG GGGCATGGCC
    GCCAAGGCCT CCTACCTGGC CGGCCTGGGC GTGAACGCGG TGGAGCTGCT GCCGGTGTTC
    GAGTGGGACG AGCTCGAGTT CCGCCGCACC CGCAACCCGC GCGACCACAT GACCAACGTC
    CTGCACGCGC AGGGCATCCA GGTGCTGCTG GACGTGGTGT ACAACCACAC GGCCGAGGCG
    GACGACAAGG ACCCCTACAC GCTGTGCATG CGCGGCGTGG ACAACAAGAC CTACTACATG
    ATCGACCCCA GCCAGTACGT GCAGCTGGTC AACTGGAGCG GGTGCGGCAA CACCGTCAAC
    GCCAACAACC CGCCCGTCAC GCAGATGATC ATCGACAGCC TGGTGCACTG GGTGACGGAG
    TACCACGTGG ACGGCTTCCG CTTCGACCTG GCCAGCTGCC TGTGCAGGGA CGAGCGCGGC
    CACCCCATGG CCGTGCCGCC GCTCATCCGC GCCATCAGCA AGCACCCGCT GCTGAGCCAG
    GTGCACTTGA TCGCCGAGCC CTGGGACATC GGCATGTACC AGGTGGGCAG CTTCCCCAAC
    TGGGACATCT GGGCGGAGTG GAACGGCAAG TACCGCGACG ACGTGCGCCG CTTCATCAAG
    GGAGACGCAG GCATGAAGCG CGCCTTCGCC ACGCGCCTGG CCGGCTCCGC CGACCTGTAC
    CAGACCAACA ACCGCAAGCC CTACCACTCC ATCAACTTCA TCACCGCGCA CGACGGCTTC
    AGCCTGTACG ACATGGTGTC CTACAACGAC AAACACAACG ACGCCAACGG CGAGGGCAAC
    CGCGACGGCA CCAACGACAA CTTCTCCTGG AACTGCGGCG CCGAGGGCGA GACCGGCAAC
    GGCGGCGTGC GGGCGCTGCG CCAGCGGCAG ATGCGCAACT GCATGGTGGC GCTCATGATG
    AGCCAGGGCA CACCCATGAT TGTCAGCGGC GATGAGATCA TCAAGACGCA CGGCGGCAAC
    AACAACTGGT ACGGCCACGA CACAGCCATG GCGCACCTGC AGTGGCCCGA GGGCGACGCC
    GACAAGGAGG CGCTGCTGCG CTTCTGCTCC CAGCTCATCG CCTTCCGCAA GAGCCACCCC
    GCGCTGGGGC GGGAACACTT CCTGGGCCCC AACGACATCA CCTGGCACGA GGACAACTGG
    GGCAACGACG AGAGCCGCTT CCTGGCCTTC ACGCTGCACC ACCGCGAGGC TGGCGACATC
    TACGCCGCCT TCAACGCCCA CGCCTTCGCC GTGTCCGCGC CGCTGCCGCA CCCGCCCGCC
    GGCCGCAAGT GGTGCCGCCT GGTGGACACC AACCTGCCGC CGCCCAAGGA CTTCACGCCC
    GGCGGCAACG CGGGTGTGGA TGCGGTGTAC GGCGTGCAGG CGTTCTCGTC AATTGTGCTT
    ATCGCCAAGC CGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The Chlamydomonas genome reveals the evolution of key animal and plant functions.
    Science (New York, N.Y.)318(5848)245-50(2007 Oct)
    Merchant SS,Prochnik SE,Vallon O,Harris EH,Karpowicz SJ,Witman GB,Terry A,Salamov A,Fritz-Laylin LK,Mar?chal-Drouard L,Marshall WF,Qu LH,Nelson DR,Sanderfoot AA,Spalding MH,Kapitonov VV,Ren Q,Ferris P,Lindquist E,Shapiro H,Lucas SM,Grimwood J,Schmutz J,Cardol P,Cerutti H,Chanfreau G,Chen CL,Cognat V,Croft MT,Dent R,Dutcher S,Fern?ndez E,Fukuzawa H,Gonz?lez-Ballester D,Gonz?lez-Halphen D,Hallmann A,Hanikenne M,Hippler M,Inwood W,Jabbari K,Kalanon M,Kuras R,Lefebvre PA,Lemaire SD,Lobanov AV,Lohr M,Manuell A,Meier I,Mets L,Mittag M,Mittelmeier T,Moroney JV,Moseley J,Napoli C,Nedelcu AM,Niyogi K,Novoselov SV,Paulsen IT,Pazour G,Purton S,Ral JP,Ria?o-Pach?n DM,Riekhof W,Rymarquis L,Schroda M,Stern D,Umen J,Willows R,Wilson N,Zimmer SL,Allmer J,Balk J,Bisova K,Chen CJ,Elias M,Gendler K,Hauser C,Lamb MR,Ledford H,Long JC,Minagawa J,Page MD,Pan J,Pootakham W,Roje S,Rose A,Stahlberg E,Terauchi AM,Yang P,Ball S,Bowler C,Dieckmann CL,Gladyshev VN,Green P,Jorgensen R,Mayfield S,Mueller-Roeber B,Rajamani S,Sayre RT,Brokstein P,Dubchak I,Goodstein D,Hornick L,Huang YW,Jhaveri J,Luo Y,Mart?nez D,Ngau WC,Otillar B,Poliakov A,Porter A,Szajkowski L,Werner G,Zhou K,Grigoriev IV,Rokhsar DS,Grossman AR