ERG25 cDNA ORF clone, Chlamydomonas reinhardtii

The following ERG25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ERG25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCh236931 XM_001696535.2
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Chlamydomonas reinhardtii sterol desaturase (ERG25), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCh236931
    Clone ID Related Accession (Same CDS sequence) XM_001696535.2
    Accession Version XM_001696535.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-08-28
    Organism Chlamydomonas reinhardtii
    Product sterol desaturase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001843733). On Aug 29, 2017 this sequence version replaced XM_001696535.1. COMPLETENESS: incomplete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGAGCTCG CGAAGCAGGC GCTGTCCCAG ATATCCCTTG TGCCGCTGTA TGGCGGGGGC 
    TTCTGGGTTG CTTTCACGCA GCTGGTGGTG TTCTATTACA GCGTTGGCGC GCTTCTGCAC
    AGCGTACTGC CACGCGTCAT CGCTGTGCAC GGCATCCAAC AGCAGCAGCG CAAACAGGGC
    GAGGTGCTGC GGGACGCCTT CTACAGCTTA GGTCCGCTCG CTGTCAAGGC CGGCGTGTGG
    GCGGCGGTGG AGGTGCTGCA TGCGCGCGGG CTGACGCTCA CCTACACCGG CCGCGTGTCG
    GGGTGGACGG CGTGGCTGTA CGTGGCGCTG TGTGTGGCGC TGCTGGACTA CCTGCACGAC
    ACCTGGTTCT ACTGGACACA CAGGCTGCTG CACTGGAAGC CGCTGTACGT GCACGTGCAC
    TACATCCACC ACAAGTCCAA GGCCCCCTCG GCCTACACCG GGTACTCCTT CCACCTGGTG
    GAGGCGCTCA TCGTGTTCGC CAACGAGGTG CTGGTGTGCT TCCTGTTCCC GCTGCACATG
    GGCCTGCACC GCGCCTACCA CTTGTACACC ACACTCATAC ACGAGGGCGG GCACGTGGGT
    TACGAGCTGG CTCCCTTCAT CCCCACCTTT GCCGGTGTGG CCACGCTGGC CTTCCGCGGC
    GTGCGGCCCT GCGCCGCCCT CAACACGGTG CAGCACCACG ACATGCACCA CCGCTACCCC
    ACCAAGCACT TCTCGCTCTA CTTCACGCAC TGGGACCGGC TGTGCGGCAC ACTGCACCCC
    GCCTACGACC GCGACCTGTT TCACTACTTC CCGACCAAGG CCGCGGCGGG TGCTGCTGCC
    GGTGCTAACG CAGGGCAGCA GGACAAGGGC GCGCAGCTGC AGAGCGGGGC GGCGGCGACC
    AACGGCGGCG TGAAGCACGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001696587.1
    CDS339..1262
    Translation

    Target ORF information:

    RefSeq Version XM_001696535.2
    Organism Chlamydomonas reinhardtii
    Definition Chlamydomonas reinhardtii sterol desaturase (ERG25), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001696535.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGAGCTCG CGAAGCAGGC GCTGTCCCAG ATATCCCTTG TGCCGCTGTA TGGCGGGGGC 
    TTCTGGGTTG CTTTCACGCA GCTGGTGGTG TTCTATTACA GCGTTGGCGC GCTTCTGCAC
    AGCGTACTGC CACGCGTCAT CGCTGTGCAC GGCATCCAAC AGCAGCAGCG CAAACAGGGC
    GAGGTGCTGC GGGACGCCTT CTACAGCTTA GGTCCGCTCG CTGTCAAGGC CGGCGTGTGG
    GCGGCGGTGG AGGTGCTGCA TGCGCGCGGG CTGACGCTCA CCTACACCGG CCGCGTGTCG
    GGGTGGACGG CGTGGCTGTA CGTGGCGCTG TGTGTGGCGC TGCTGGACTA CCTGCACGAC
    ACCTGGTTCT ACTGGACACA CAGGCTGCTG CACTGGAAGC CGCTGTACGT GCACGTGCAC
    TACATCCACC ACAAGTCCAA GGCCCCCTCG GCCTACACCG GGTACTCCTT CCACCTGGTG
    GAGGCGCTCA TCGTGTTCGC CAACGAGGTG CTGGTGTGCT TCCTGTTCCC GCTGCACATG
    GGCCTGCACC GCGCCTACCA CTTGTACACC ACACTCATAC ACGAGGGCGG GCACGTGGGT
    TACGAGCTGG CTCCCTTCAT CCCCACCTTT GCCGGTGTGG CCACGCTGGC CTTCCGCGGC
    GTGCGGCCCT GCGCCGCCCT CAACACGGTG CAGCACCACG ACATGCACCA CCGCTACCCC
    ACCAAGCACT TCTCGCTCTA CTTCACGCAC TGGGACCGGC TGTGCGGCAC ACTGCACCCC
    GCCTACGACC GCGACCTGTT TCACTACTTC CCGACCAAGG CCGCGGCGGG TGCTGCTGCC
    GGTGCTAACG CAGGGCAGCA GGACAAGGGC GCGCAGCTGC AGAGCGGGGC GGCGGCGACC
    AACGGCGGCG TGAAGCACGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The Chlamydomonas genome reveals the evolution of key animal and plant functions.
    Science (New York, N.Y.)318(5848)245-50(2007 Oct)
    Merchant SS,Prochnik SE,Vallon O,Harris EH,Karpowicz SJ,Witman GB,Terry A,Salamov A,Fritz-Laylin LK,Mar?chal-Drouard L,Marshall WF,Qu LH,Nelson DR,Sanderfoot AA,Spalding MH,Kapitonov VV,Ren Q,Ferris P,Lindquist E,Shapiro H,Lucas SM,Grimwood J,Schmutz J,Cardol P,Cerutti H,Chanfreau G,Chen CL,Cognat V,Croft MT,Dent R,Dutcher S,Fern?ndez E,Fukuzawa H,Gonz?lez-Ballester D,Gonz?lez-Halphen D,Hallmann A,Hanikenne M,Hippler M,Inwood W,Jabbari K,Kalanon M,Kuras R,Lefebvre PA,Lemaire SD,Lobanov AV,Lohr M,Manuell A,Meier I,Mets L,Mittag M,Mittelmeier T,Moroney JV,Moseley J,Napoli C,Nedelcu AM,Niyogi K,Novoselov SV,Paulsen IT,Pazour G,Purton S,Ral JP,Ria?o-Pach?n DM,Riekhof W,Rymarquis L,Schroda M,Stern D,Umen J,Willows R,Wilson N,Zimmer SL,Allmer J,Balk J,Bisova K,Chen CJ,Elias M,Gendler K,Hauser C,Lamb MR,Ledford H,Long JC,Minagawa J,Page MD,Pan J,Pootakham W,Roje S,Rose A,Stahlberg E,Terauchi AM,Yang P,Ball S,Bowler C,Dieckmann CL,Gladyshev VN,Green P,Jorgensen R,Mayfield S,Mueller-Roeber B,Rajamani S,Sayre RT,Brokstein P,Dubchak I,Goodstein D,Hornick L,Huang YW,Jhaveri J,Luo Y,Mart?nez D,Ngau WC,Otillar B,Poliakov A,Porter A,Szajkowski L,Werner G,Zhou K,Grigoriev IV,Rokhsar DS,Grossman AR