CHLREDRAFT_146309 cDNA ORF clone, Chlamydomonas reinhardtii

Gene
Clones
gRNAs

Summary

Gene Symbol	CHLREDRAFT_146309
Entrez Gene ID	5715156
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Chlamydomonas reinhardtii

mRNA and Protein(s)

mRNA	Protein	Name
XM_001689884.1	XP_001689936.1	predicted protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Chlamydomonas genome reveals the evolution of key animal and plant functions.
Merchant SS, Prochnik SE, Vallon O, Harris EH, Karpowicz SJ, Witman GB, Terry A, Salamov A, Fritz-Laylin LK, Mar?chal-Drouard L, Marshall WF, Qu LH, Nelson DR, Sanderfoot AA, Spalding MH, Kapitonov VV, Ren Q, Ferris P, Lindquist E, Shapiro H, Lucas SM, Grimwood J, Schmutz J, Cardol P, Cerutti H, Chanfreau G, Chen CL, Cognat V, Croft MT, Dent R, Dutcher S, Fern?ndez E, Fukuzawa H, Gonz?lez-Ballester D, Gonz?lez-Halphen D, Hallmann A, Hanikenne M, Hippler M, Inwood W, Jabbari K, Kalanon M, Kuras R, Lefebvre PA, Lemaire SD, Lobanov AV, Lohr M, Manuell A, Meier I, Mets L, Mittag M, Mittelmeier T, Moroney JV, Moseley J, Napoli C, Nedelcu AM, Niyogi K, Novoselov SV, Paulsen IT, Pazour G, Purton S, Ral JP, Ria?o-Pach?n DM, Riekhof W, Rymarquis L, Schroda M, Stern D, Umen J, Willows R, Wilson N, Zimmer SL, Allmer J, Balk J, Bisova K, Chen CJ, Elias M, Gendler K, Hauser C, Lamb MR, Ledford H, Long JC, Minagawa J, Page MD, Pan J, Pootakham W, Roje S, Rose A, Stahlberg E, Terauchi AM, Yang P, Ball S, Bowler C, Dieckmann CL, Gladyshev VN, Green P, Jorgensen R, Mayfield S, Mueller-Roeber B, Rajamani S, Sayre RT, Brokstein P, Dubchak I, Goodstein D, Hornick L, Huang YW, Jhaveri J, Luo Y, Mart?nez D, Ngau WC, Otillar B, Poliakov A, Porter A, Szajkowski L, Werner G, Zhou K, Grigoriev IV, Rokhsar DS, Grossman AR
Science (New York, N.Y.)318(5848)245-50(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CHLREDRAFT_146309 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHLREDRAFT_146309 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCh00196	XM_001689884.1 Latest version!	Chlamydomonas reinhardtii predicted protein (CHLREDRAFT_146309), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHLREDRAFT_146309 ( XM_001689884.1 ) cDNA ORF clone, Chlamydomonas reinhardtii -> XP_001689936.1 Chlamydomonas reinhardtii predicted protein (CHLREDRAFT_146309), partial mRNA.

Price & Availability↑

CloneID	OCh00196
Clone ID Related Accession (Same CDS sequence)	XM_001689884.1
Accession Version	XM_001689884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-28
Organism	Chlamydomonas reinhardtii
Product	predicted protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001843471). COMPLETENESS: incomplete on the 5' end.

ATGTACGACC TGTGGGCCAC CGAGGCGTCA GCATCGGGGC TGACTGGTGC AAAGCGGCGG 
AGCGGCGCCG TCGCTGCTGC CGCGCCCGGC GGAGTTGCCG CACGGGCTGG CGGTGGTGGT 
GGGCCGCCGG CCGCCAAGCG CGCCAAGCAA GAGGCTGGGA CACCGCGGGC TGCCGAGGCT 
TCCAAGGCGC AACCAAAAGC AGCAGCTGCG GCTGCTGCGG GCAGCAGCCG ACGGCCTCCA 
CGCAGCGCCG CTGCCGCTGC GGCAGTAGCC GATGCTGACG CAAAGCCTGA GTCCACGGCA 
CCGAAGTCCG CAGCCAAGTC GAAAGGCAAG ACTACGGCAG AGGACGCGGG CGCCACCAGC 
AAGCAGCCCG CCCCCAAGGG CAAGACTACA GCCAAAGCAG CGGAGAACGA CGCTGACGAG 
GACAAGGCAG CTGACGCTGC TCAGGCCACC GCAGTAGCTG CGAAAAAGGA TGATGCCGCA 
GGGGGCAAGA AGGGCAAAGC AGTAGCTAAG AAGGCGGGGG CTGCGGAGGC TGCCAGCGGC 
GAAGCAGCGG GCGACAAGAA CGGCAAGGCA ACGGCCAAAG CTGGCAAGGG CGAGGCAGCG 
GGAGTCAAGG CGGAAGCAGC AGAGCCGCAG TATAGTGGGG CAGCTGACGC AAAGCGTGCA 
GGCGGAGCCA AGGGCAAGGG CGCGCAGCAA GCGAAAAAGA AGGATGGCGC TGCCGATGCC 
GAGACCGCTG CCGCTACGAC GTCTGAGGCT GAGGAGGTGA AGCCGGAGCC AGGGGAGCCC 
AAGCCGCAGG GCAAGCGGAA GGCGGCAGCT GCAAGCGGGA AGAAGGGCAA AGGGCACGGC 
CAGGAGGAGG TGGTGGAACC TAAGGAGGAG AAGGAGGAGG CTGTAGCGGG TCCGTCGACA 
GCGGCAGCAG CGGACTCGCA GGCAACGGAC GGTGGCAAGC CGGCTGCCTC CACCGCCGCA 
GCGTCGGAGA AGCAAGCGGC CAGCGGCAAA GGCAAGCGGG GTGCGGCGGC GGCTGATGCT 
AAGAAGCCGG CCGCCCGAAC TCCATCCCGG AAGACAGCTT CGGCCTCGGC AGCGTCCAAG 
GACAATGCTG CCGCTGCGGG CAGCAACAAG GAGGCTACGT CACCTGGCGA GGGCGTGAAG 
ACGGCAGAGG CAACGGAGGA TGCGGAGATG AAGGATGCGG AGGTCAACGC GGAGGCACCG 
GCGGTGGGTA GCGAAGGCGC TGGTGGGAAG GACGCTGCGG CTGCCGCAGG AAGCAGCAAA 
GAAGAGCATG GCGGCGAGGC CGCTGGCAGC AAGGACGCAG CCGCTGCTTC TGCCAAGGCT 
GGCGAACGCA GCACCCGCCT GCCACGGCGC AGCAAGCTGC TCAAGCCGAC ACCCGCAGAC 
GCAGCACCGA AGCCGGCGGG AGCGGACGCT ACTGCGGCCG GTACGGCAGA GCCGGCAGCT 
GATGCTGCCG CCATGTCCGC CAAACTGGCG GGTGGTGATA ACGAGGCAGT GGCCGGCGTC 
AAGGCCGAGG CCGCAGACGA GCCGGCCGCT GATGCTGCAG CGTCTAAGGA CGACGACGCC 
GGCGACGCAA CTGCGCCCAA ACCGCCGCGG CCACCGCCGC CGCCTGGTCC ACCGGGCTTC 
AAGGAGGACC CTGACTGGCT GGACTGGCCC ATCAAGCTGA CGGACGAACA TGTGGACTTC 
ATCCGCACAC TGGCGCACAA GGTGTTCGCG GCCAAGGAGG CGCCGCCGCG CGACGCCAAG 
GCGCTGCTGC GCTGCTCGGG CATGTGGGGG CGCAAGCTCG AGTACCGCAA GCGCGACGGT 
CGTGAGGGCA CCTACCGGCC CATCTTCAGC GGCATCGTGA CGGACAAGGG CACGCTGGCC 
TGCACCTGCG AGGCCTGCAA GGAGGAGAGG ACGCACTCGC TGACCGAGTT CGAGGTGCAC 
TGCGGCGGCA CGCACAAGAA GCCGGCAGAC CACACATGGG TCATCGAGCT GGGCCTCAAC 
CTGCGAGACC TGGCGCTCAT GACCTGCCGC TGCCTGGGCC TGGGCCACAT CGCCGCCGGC 
ACCGGCCAGG GCCGCATCAG CGGCGCCGCC GTAAAGGCTG CGCTGGGCGA GTCCGCGCTG 
GGCACGGGCG AGGGGAAGAA GCGCAGCGGC GGCGGCAAGC CCCGCAAGTC TGCTGCCGCT 
GCCGCGGCAG CCGGCGGCGC TGAAGGTGAG GGCGGCACAG CTGCCCTAGT CAGCTCGCGC 
CCACGCGGCA CAGGCGGCAG CCCGGCAGGC CGCAAGCCCG GCAGGCTTCG CGCCGCCGGC 
TCCAGCACCG AGACGGAGTC CGACGTGGAG ACCGAGACGG AGACTGAGGA TGACCTGGAC 
GACTTCGAGG AGCAAGTGGA GCGGACGGCG CCCACCGGCG GCACAGCCCG CCAGCGCGCC 
GCTGCCGCGC AGGGGGCCGC GGCCGTGGCT ACCGCGGGCG TAGCGGGATG GGACCGCCAC 
GCGCCCGTGC CGGCGGGCAC TTGCACGGTG TGCTTGCGGG CGCTGCCAGA AAACTACAAG 
GAGCTGGCGG CAGCGCCCGT CAACGCGACC GTCCTTTCCT GCTCCAAGTG CCAGGGCCAC 
TGCCACACCT CCTGCGCGGC GCCTTTCGGC TTGCCGCCAC CGAAGCCAGC CACGGCTGCA 
GCTCCGGCCG CAGCGCCCGC TGTGGACGCG CCCGCTCCAC AGCCCGCGGC GGCGCCTGAG 
CAGGCACCCG AGGGCGCTGC CCCTAGCACC CAGCCCGCCG CGGCAGCGCC CGCGGCAGCG 
CCCGCGGCTG ACGCCGCCCC AGTGGCAGAC GCGGCACAGG CAGCCGACGC AGTCATGGTG 
GAGGCGGGCG AGCCAACCGC ACAAGCTGCA GACGCTCCTT CCGTCGACGC CGCAGCCCCG 
GCCCCGGCCC CGGCCGATGC GCCTGCTGCT GTTGCCGCGG AAGCTTCAGC TGCGCCGGCC 
GCTGACGCGT CCGCTGACGC GCCTGCTGTC GCCGCTGCTG CAGACACAGC TGCCGTTGCC 
ACCGACGCTG CCATTTCTGT GGCGGCCACC TGCGACTGGC AGTGCCCCCA TTGCCGCCAG 
GTGGGCCGCG GCAAGCGCAT GAAGCGCCCC AACCCCACCT ACTTAGACCA AGACGCTGCA 
GCGACAGCTA CCGCCGCCGG CCCCGCACCC GAGCGGCTAG GGCGCGGGCG CGGGCGCGGC 
GGCAGGCCGC CGGGCCCCGG TGTTGCAATC GGCGGTGGCC GCGGGCGCGG TCGGGGCCGC 
GGGCGCAACG TCATGAACAT GACGCAGGCT GAGATCGCGG AGATGTACTT CTCCAAGGCG 
CGCATGGCAA TGGCAAAGGG CGGCGACGGC ACCATCCCCA AGCCCACGTT CAGCAGCGGC 
GCCGGCGGCG CCTTGGGTGG CCTGGACGGC GCCGCGCCGG CGGCAGAGCG TGTGCCTGCA 
GCTGAGGGTG CCGCCGAAGC GGCTGCGACA GCGCCTGCCG AAGGCGCAGC CGCGCACCCA 
CCCGCCACGG CGCCAGGTGG CGCTGTGACA GCAGCACCAA CTGATACCGC TGCCGCCGGA 
GGCGAGGTGG CCGCGGCCGG GACTTCTGGA ACCGCCGCGC AGGTGCCCAC GCAGCCAGGG 
GACGCGGCCG CCACATCTGC TGCTCCTGCC GACGTAGACG CAGCCAAGGA GAAGGCCAAG 
CGCGAGCAGG AGGACGCGGC GCTCGCACGC GCCATCGCCA TCGCCGAGGG CTGGGGCGAG 
GAGGAGTATG GGGAGGAGTA CGGTGAGGAG GATGAGGAGG ATGACGACGA TGAGGACGAG 
GAGGAGGAGG CGGTGGACGA GGACGACGAG GACTATGAGG GCGCCCGGCG GCGCAAGCGG 
CCGAAGGCCG CCGTCGCGCC GCCGCCGCGG ACGCATTTTA GCATGGCGCC GCCGCCAATG 
CCGTCGCCCG TGCCCCGGCC GGCTGGCGCC CCTGCGGGTG CTGCGCCGGG GCCCGCAGGT 
GGCGGCGGCC GTATACAGAT GACGCTGCCG GTAACCATGC GCGCGTCCGC GGCTCGCAGC 
AACGCCTCGG CGCGGCGGCA GGTGTGCGGC CGGACGCTGT TCGACGGCCT GCCAGGCGGC 
CTCAAGCACG ACGACATCGT GTGGTACACG CTGCAGGGCA AGCGGCTCCT GCGCGGCGTG 
GTTCAGCTGC AGGCGTGTGG CGCCGCGACC AGCGGCATCC TGTGCGGCCA CTGCGACACC 
ATCATCAGCG CCTCCGCCTT CGAGGCGCAT GCAGGCCACA AGCACCGGCG CAACCCGTAT 
GAGGCCATCC TCACCGATGA CGGCATGACG CTGAAGCGTA TCGCCGAGCG CCTCATGCCC 
ATTGCCGCCA GCCAGGCAGG CCACGCCTCC CCGCCACAGC CGCGCGCCAA GGGCGCTGGC 
GGTGCCCTGG CGGCCGCCGC CGCCGCGGTC GCGGCCGCAG CTCACGCCGC AGCGGCAGTG 
CATCACGATG ATCACCAACA CCACCACGAC GGCGGTGAGG CCGGCGAGGA CGACGACGAC 
GTGGAGATGG TCGAGCTGGT GGATGACGCT GCCTGCGTGC TGTGCCACGA GCCGGACTTT 
GACCGGGAGG GCTTCAGCGA CAAGACCGTG CTTATCTGCG ACCAGTGCGA GAAGGAGTAC 
CACATCGGCT GCCTGCGTCA GCACAAGATG GTGGACATGC AGGCGGTGCC GGAGGGCGAA 
TGGTTCTGCA GCGACGAGTG CGTGCGCATT CGCGATGCGC TGGGCGAGGA CGTGGCGGCG 
GGCGAGGTGC TCATGCCTGG CAACCCCGCC TACCGCTGGC AGATCCTGCG CGGCAAGAAT 
GGCCGGCAGC AGACCTGGCA CGCGCTGTCC ACTGTGCTCA ACATCCTTCA GGAGTCGTTC 
GACCCCATCA TCGACACCGG CAGCGGCTCC GACCTGCTGC CGGCCATGGT CAACGCCGAG 
ACGGCGGGCG ACTACGACTT CCAGGGAATG TACTCCATCC TGCTGCGGTA CAGGGGCCCG 
GACAAGGAGG CGCGCGGCAA GCCGGTGCTG GCGGCGCTGT TCCGCGTGCT GGGCAGCAGT 
ATGGCGGAGA TGCCGCTGGT GGCCACCCGC TACGACTGCC GCCGCCAGGG CCACCTGCGC 
GCGCTGGTGG ACGCGATGCG CCACAAGCTG CTGGGGCTGG GCGTGCGCGC CATCGTTCTG 
CCCGCCACGG CCGACGCGCA ACCCGCCTGG CGCCAGCTGC AGTTCCAGGA CCTGGACGAG 
CCGTCCACAC GCGTGGCGCG CAGCGAGCAC CGCATGGTCA TCTTCCCGCA CACCACCGTG 
CTGGCGCGCC CGCTCATCCG CGTGCCTGGC TACGACCAGA GCCAGCTGCA GTCCGCGCAC 
GAGGCGGCCG ACCCAGCATT CATCGCCGCC TGCGCCAAAG CCATGGAGGA GGCCCGCGTC 
GCCTCCGGCA AGGAGATCGT GGACAGCGAG GAGAAGATCT ACGCCGCTAT GGACGACGCG 
GCGGCCGCCG CCGCGGAGGT GCAGGCGGCC AAGGCGGCCG CGCTGGCGGA GCAAGGCATT 
TTCGTAGGCC GCGGCCGAGG CCGCGGCCGC GGGCGGGGGC GAGGTCGTGG TTTTGCGGCG 
GCCGCCGCCA CCGGAGGGGG CTACGGTGCG CCATACAGCG CCGCCGGGCC TGTGGAGGTG 
GATGCCTACG GGCAGCCGGT GGAGGGCGAG CCGTACAGCG GCGCCGGAGG GCCATACCCT 
GGCGCGCCGC CGCACTTGGC GCTGCTGCCG GGCTCGCCGG GCGCACCGGG CCGGGGGCGC 
GGCCGGGGGC GCGGGCGCCG CGGGCGGCCT CCCATGTCGG CCCATGTCGC GTACCCGCAC 
CCCGGCGCTG TCGCCGGCGA CCCAGGCGCC GGCGATTATC CGGCCGCACC GCCCGAGGGT 
GATGACGGCA TGGCGGCGTA CGGCGAAGGC GCGGGCGAGG GCGATGACGG CGAGGAGGGC 
GACGACACCT GGGCCGGCTA CGGCGGCGTC GGTGAGGTGG CGTCGGCGGT GGGCAGCGCT 
GTTGCTGCAG GCGAGACGGG CGAGGGCATG ATGACGGCGG CCATGGGCGC GGCGTCACGC 
TTCTCCATGG GCCCGCTGCA AGGCGCCATG GTGGCAGCTG CGGCCGCGGC CGCGGCAGCG 
GCGGCAAAGC AGTACGCGCC GCAGATGGCG GCGGTGGATG CGGCGGGCAG GCCGCGGCAG 
CCATCCGACG GCACGGATGC CGCTGGCATG CCGGCAGCGG CCTTTGGCAT GCCGCCGCCT 
CAGCAGCCAG GCCAGGGGCT TGCAGCCTTC GGCGCGGCCG CATCTGGGGC GTCCGTAGCC 
GGCATGGCGC CCGGCTACGG TGCGGATGCT GCCGGGCAAG CTGGAGCGCA GGCGCCCGCA 
GCGGTGGCTG CGGCTGCGGC GGCTTACAAG TGGGCGCTGG AGCAGTTCAC GCAGAAGCAG 
CACCAGCAGC AACCGCCAGC ACCGGCAGCC GGCCCTGGGC CGGAAAGCAC AGGCGCCGCA 
GCGTACGGCA TGGCGGCGCG GGCTTACGGC AACGCGATGA ATGCTGGGAT GGCGGCGGCG 
ACAGCAGCGC GCACTAGTGG CGGCGGCGCC CCGCCTACCG GCGCGTGGAG CAGCCAGCCG 
CCGGGCTGGC CGCAGCCGCA GCAGGTGCAC TACCAGCCGC CGACATCCCA GCCGCCGCAG 
CCAGTGTCCT TGCAGCCGAG CCAGCAGCAG CTGCAGGCCT GCAATTTCCT TGCAAGCCTA 
ACGGGCGGCG GCGGCCTCTC TAGTGGTGGT GCGCCGCCGG GCCCTGCGGC AGGCGCGCCA 
GGGAGCAGCT GGGGAGCTCC TCCGGCGCAA GCGCCGCAGG CGGCTCAGCA TTCCTACCAG 
CCACCGCCGC AGCCGCAGCC ACCGCAGCAG CACCATCACC AACCGCACCA GCTGCACCAG 
CAGCCGCACC AGATGCAGGG CGGCATGGTG GCTTACCACC AAACGCACCA GCAACCATTG 
TCAGCGGCGC AGCCGCAGCA CTATCAGCAG CAGCCTGGCG CGTGGCCCCA AGCGGCTGCG 
GGCGGCAGCA ACCCATCATT GACAGACCAG AACTACCACG AGACGCTTGC GGCACTGCAG 
CGGCAGCAGG AGCGGCAAAA CCAACAAGCG TTGCTGGGGA TACCGCCGGC TCAGCAGCAA 
CAAGCACAGC AGCACCCGCA GTTCCCGCAG CATGGGGGTT ATCCTACCGG GATTGCGCCG 
CCGCAGCAGC CGCCGCAGCA GCAACAGCAG TATGGGTATC CTGACCCGCA GGCGGCTGCG 
CAGCAGGCGG CTGCGCAGTT CGCTGCGTAC GGGCAGTACG GGGGGCCGGC TGGGGCGCCA 
GGGCTGTTCG GCCAGCAGCC GCAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001689936.1
CDS	1..7350
Translation	MYDLWATEASASGLTGAKRRSGAVAAAAPGGVAARAGGGGGPPAAKRAKQEAGTPRAAEASKAQPKAAAAAAAGSSRRPPRSAAAAAAVADADAKPESTAPKSAAKSKGKTTAEDAGATSKQPAPKGKTTAKAAENDADEDKAADAAQATAVAAKKDDAAGGKKGKAVAKKAGAAEAASGEAAGDKNGKATAKAGKGEAAGVKAEAAEPQYSGAADAKRAGGAKGKGAQQAKKKDGAADAETAAATTSEAEEVKPEPGEPKPQGKRKAAAASGKKGKGHGQEEVVEPKEEKEEAVAGPSTAAAADSQATDGGKPAASTAAASEKQAASGKGKRGAAAADAKKPAARTPSRKTASASAASKDNAAAAGSNKEATSPGEGVKTAEATEDAEMKDAEVNAEAPAVGSEGAGGKDAAAAAGSSKEEHGGEAAGSKDAAAASAKAGERSTRLPRRSKLLKPTPADAAPKPAGADATAAGTAEPAADAAAMSAKLAGGDNEAVAGVKAEAADEPAADAAASKDDDAGDATAPKPPRPPPPPGPPGFKEDPDWLDWPIKLTDEHVDFIRTLAHKVFAAKEAPPRDAKALLRCSGMWGRKLEYRKRDGREGTYRPIFSGIVTDKGTLACTCEACKEERTHSLTEFEVHCGGTHKKPADHTWVIELGLNLRDLALMTCRCLGLGHIAAGTGQGRISGAAVKAALGESALGTGEGKKRSGGGKPRKSAAAAAAAGGAEGEGGTAALVSSRPRGTGGSPAGRKPGRLRAAGSSTETESDVETETETEDDLDDFEEQVERTAPTGGTARQRAAAAQGAAAVATAGVAGWDRHAPVPAGTCTVCLRALPENYKELAAAPVNATVLSCSKCQGHCHTSCAAPFGLPPPKPATAAAPAAAPAVDAPAPQPAAAPEQAPEGAAPSTQPAAAAPAAAPAADAAPVADAAQAADAVMVEAGEPTAQAADAPSVDAAAPAPAPADAPAAVAAEASAAPAADASADAPAVAAAADTAAVATDAAISVAATCDWQCPHCRQVGRGKRMKRPNPTYLDQDAAATATAAGPAPERLGRGRGRGGRPPGPGVAIGGGRGRGRGRGRNVMNMTQAEIAEMYFSKARMAMAKGGDGTIPKPTFSSGAGGALGGLDGAAPAAERVPAAEGAAEAAATAPAEGAAAHPPATAPGGAVTAAPTDTAAAGGEVAAAGTSGTAAQVPTQPGDAAATSAAPADVDAAKEKAKREQEDAALARAIAIAEGWGEEEYGEEYGEEDEEDDDDEDEEEEAVDEDDEDYEGARRRKRPKAAVAPPPRTHFSMAPPPMPSPVPRPAGAPAGAAPGPAGGGGRIQMTLPVTMRASAARSNASARRQVCGRTLFDGLPGGLKHDDIVWYTLQGKRLLRGVVQLQACGAATSGILCGHCDTIISASAFEAHAGHKHRRNPYEAILTDDGMTLKRIAERLMPIAASQAGHASPPQPRAKGAGGALAAAAAAVAAAAHAAAAVHHDDHQHHHDGGEAGEDDDDVEMVELVDDAACVLCHEPDFDREGFSDKTVLICDQCEKEYHIGCLRQHKMVDMQAVPEGEWFCSDECVRIRDALGEDVAAGEVLMPGNPAYRWQILRGKNGRQQTWHALSTVLNILQESFDPIIDTGSGSDLLPAMVNAETAGDYDFQGMYSILLRYRGPDKEARGKPVLAALFRVLGSSMAEMPLVATRYDCRRQGHLRALVDAMRHKLLGLGVRAIVLPATADAQPAWRQLQFQDLDEPSTRVARSEHRMVIFPHTTVLARPLIRVPGYDQSQLQSAHEAADPAFIAACAKAMEEARVASGKEIVDSEEKIYAAMDDAAAAAAEVQAAKAAALAEQGIFVGRGRGRGRGRGRGRGFAAAAATGGGYGAPYSAAGPVEVDAYGQPVEGEPYSGAGGPYPGAPPHLALLPGSPGAPGRGRGRGRGRRGRPPMSAHVAYPHPGAVAGDPGAGDYPAAPPEGDDGMAAYGEGAGEGDDGEEGDDTWAGYGGVGEVASAVGSAVAAGETGEGMMTAAMGAASRFSMGPLQGAMVAAAAAAAAAAAKQYAPQMAAVDAAGRPRQPSDGTDAAGMPAAAFGMPPPQQPGQGLAAFGAAASGASVAGMAPGYGADAAGQAGAQAPAAVAAAAAAYKWALEQFTQKQHQQQPPAPAAGPGPESTGAAAYGMAARAYGNAMNAGMAAATAARTSGGGAPPTGAWSSQPPGWPQPQQVHYQPPTSQPPQPVSLQPSQQQLQACNFLASLTGGGGLSSGGAPPGPAAGAPGSSWGAPPAQAPQAAQHSYQPPPQPQPPQQHHHQPHQLHQQPHQMQGGMVAYHQTHQQPLSAAQPQHYQQQPGAWPQAAAGGSNPSLTDQNYHETLAALQRQQERQNQQALLGIPPAQQQQAQQHPQFPQHGGYPTGIAPPQQPPQQQQQYGYPDPQAAAQQAAAQFAAYGQYGGPAGAPGLFGQQPQR

Target ORF information:

RefSeq Version	XM_001689884.1
Organism	Chlamydomonas reinhardtii
Definition	Chlamydomonas reinhardtii predicted protein (CHLREDRAFT_146309), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001689884.1

ORF Insert Sequence:

ATGTACGACC TGTGGGCCAC CGAGGCGTCA GCATCGGGGC TGACTGGTGC AAAGCGGCGG 
AGCGGCGCCG TCGCTGCTGC CGCGCCCGGC GGAGTTGCCG CACGGGCTGG CGGTGGTGGT 
GGGCCGCCGG CCGCCAAGCG CGCCAAGCAA GAGGCTGGGA CACCGCGGGC TGCCGAGGCT 
TCCAAGGCGC AACCAAAAGC AGCAGCTGCG GCTGCTGCGG GCAGCAGCCG ACGGCCTCCA 
CGCAGCGCCG CTGCCGCTGC GGCAGTAGCC GATGCTGACG CAAAGCCTGA GTCCACGGCA 
CCGAAGTCCG CAGCCAAGTC GAAAGGCAAG ACTACGGCAG AGGACGCGGG CGCCACCAGC 
AAGCAGCCCG CCCCCAAGGG CAAGACTACA GCCAAAGCAG CGGAGAACGA CGCTGACGAG 
GACAAGGCAG CTGACGCTGC TCAGGCCACC GCAGTAGCTG CGAAAAAGGA TGATGCCGCA 
GGGGGCAAGA AGGGCAAAGC AGTAGCTAAG AAGGCGGGGG CTGCGGAGGC TGCCAGCGGC 
GAAGCAGCGG GCGACAAGAA CGGCAAGGCA ACGGCCAAAG CTGGCAAGGG CGAGGCAGCG 
GGAGTCAAGG CGGAAGCAGC AGAGCCGCAG TATAGTGGGG CAGCTGACGC AAAGCGTGCA 
GGCGGAGCCA AGGGCAAGGG CGCGCAGCAA GCGAAAAAGA AGGATGGCGC TGCCGATGCC 
GAGACCGCTG CCGCTACGAC GTCTGAGGCT GAGGAGGTGA AGCCGGAGCC AGGGGAGCCC 
AAGCCGCAGG GCAAGCGGAA GGCGGCAGCT GCAAGCGGGA AGAAGGGCAA AGGGCACGGC 
CAGGAGGAGG TGGTGGAACC TAAGGAGGAG AAGGAGGAGG CTGTAGCGGG TCCGTCGACA 
GCGGCAGCAG CGGACTCGCA GGCAACGGAC GGTGGCAAGC CGGCTGCCTC CACCGCCGCA 
GCGTCGGAGA AGCAAGCGGC CAGCGGCAAA GGCAAGCGGG GTGCGGCGGC GGCTGATGCT 
AAGAAGCCGG CCGCCCGAAC TCCATCCCGG AAGACAGCTT CGGCCTCGGC AGCGTCCAAG 
GACAATGCTG CCGCTGCGGG CAGCAACAAG GAGGCTACGT CACCTGGCGA GGGCGTGAAG 
ACGGCAGAGG CAACGGAGGA TGCGGAGATG AAGGATGCGG AGGTCAACGC GGAGGCACCG 
GCGGTGGGTA GCGAAGGCGC TGGTGGGAAG GACGCTGCGG CTGCCGCAGG AAGCAGCAAA 
GAAGAGCATG GCGGCGAGGC CGCTGGCAGC AAGGACGCAG CCGCTGCTTC TGCCAAGGCT 
GGCGAACGCA GCACCCGCCT GCCACGGCGC AGCAAGCTGC TCAAGCCGAC ACCCGCAGAC 
GCAGCACCGA AGCCGGCGGG AGCGGACGCT ACTGCGGCCG GTACGGCAGA GCCGGCAGCT 
GATGCTGCCG CCATGTCCGC CAAACTGGCG GGTGGTGATA ACGAGGCAGT GGCCGGCGTC 
AAGGCCGAGG CCGCAGACGA GCCGGCCGCT GATGCTGCAG CGTCTAAGGA CGACGACGCC 
GGCGACGCAA CTGCGCCCAA ACCGCCGCGG CCACCGCCGC CGCCTGGTCC ACCGGGCTTC 
AAGGAGGACC CTGACTGGCT GGACTGGCCC ATCAAGCTGA CGGACGAACA TGTGGACTTC 
ATCCGCACAC TGGCGCACAA GGTGTTCGCG GCCAAGGAGG CGCCGCCGCG CGACGCCAAG 
GCGCTGCTGC GCTGCTCGGG CATGTGGGGG CGCAAGCTCG AGTACCGCAA GCGCGACGGT 
CGTGAGGGCA CCTACCGGCC CATCTTCAGC GGCATCGTGA CGGACAAGGG CACGCTGGCC 
TGCACCTGCG AGGCCTGCAA GGAGGAGAGG ACGCACTCGC TGACCGAGTT CGAGGTGCAC 
TGCGGCGGCA CGCACAAGAA GCCGGCAGAC CACACATGGG TCATCGAGCT GGGCCTCAAC 
CTGCGAGACC TGGCGCTCAT GACCTGCCGC TGCCTGGGCC TGGGCCACAT CGCCGCCGGC 
ACCGGCCAGG GCCGCATCAG CGGCGCCGCC GTAAAGGCTG CGCTGGGCGA GTCCGCGCTG 
GGCACGGGCG AGGGGAAGAA GCGCAGCGGC GGCGGCAAGC CCCGCAAGTC TGCTGCCGCT 
GCCGCGGCAG CCGGCGGCGC TGAAGGTGAG GGCGGCACAG CTGCCCTAGT CAGCTCGCGC 
CCACGCGGCA CAGGCGGCAG CCCGGCAGGC CGCAAGCCCG GCAGGCTTCG CGCCGCCGGC 
TCCAGCACCG AGACGGAGTC CGACGTGGAG ACCGAGACGG AGACTGAGGA TGACCTGGAC 
GACTTCGAGG AGCAAGTGGA GCGGACGGCG CCCACCGGCG GCACAGCCCG CCAGCGCGCC 
GCTGCCGCGC AGGGGGCCGC GGCCGTGGCT ACCGCGGGCG TAGCGGGATG GGACCGCCAC 
GCGCCCGTGC CGGCGGGCAC TTGCACGGTG TGCTTGCGGG CGCTGCCAGA AAACTACAAG 
GAGCTGGCGG CAGCGCCCGT CAACGCGACC GTCCTTTCCT GCTCCAAGTG CCAGGGCCAC 
TGCCACACCT CCTGCGCGGC GCCTTTCGGC TTGCCGCCAC CGAAGCCAGC CACGGCTGCA 
GCTCCGGCCG CAGCGCCCGC TGTGGACGCG CCCGCTCCAC AGCCCGCGGC GGCGCCTGAG 
CAGGCACCCG AGGGCGCTGC CCCTAGCACC CAGCCCGCCG CGGCAGCGCC CGCGGCAGCG 
CCCGCGGCTG ACGCCGCCCC AGTGGCAGAC GCGGCACAGG CAGCCGACGC AGTCATGGTG 
GAGGCGGGCG AGCCAACCGC ACAAGCTGCA GACGCTCCTT CCGTCGACGC CGCAGCCCCG 
GCCCCGGCCC CGGCCGATGC GCCTGCTGCT GTTGCCGCGG AAGCTTCAGC TGCGCCGGCC 
GCTGACGCGT CCGCTGACGC GCCTGCTGTC GCCGCTGCTG CAGACACAGC TGCCGTTGCC 
ACCGACGCTG CCATTTCTGT GGCGGCCACC TGCGACTGGC AGTGCCCCCA TTGCCGCCAG 
GTGGGCCGCG GCAAGCGCAT GAAGCGCCCC AACCCCACCT ACTTAGACCA AGACGCTGCA 
GCGACAGCTA CCGCCGCCGG CCCCGCACCC GAGCGGCTAG GGCGCGGGCG CGGGCGCGGC 
GGCAGGCCGC CGGGCCCCGG TGTTGCAATC GGCGGTGGCC GCGGGCGCGG TCGGGGCCGC 
GGGCGCAACG TCATGAACAT GACGCAGGCT GAGATCGCGG AGATGTACTT CTCCAAGGCG 
CGCATGGCAA TGGCAAAGGG CGGCGACGGC ACCATCCCCA AGCCCACGTT CAGCAGCGGC 
GCCGGCGGCG CCTTGGGTGG CCTGGACGGC GCCGCGCCGG CGGCAGAGCG TGTGCCTGCA 
GCTGAGGGTG CCGCCGAAGC GGCTGCGACA GCGCCTGCCG AAGGCGCAGC CGCGCACCCA 
CCCGCCACGG CGCCAGGTGG CGCTGTGACA GCAGCACCAA CTGATACCGC TGCCGCCGGA 
GGCGAGGTGG CCGCGGCCGG GACTTCTGGA ACCGCCGCGC AGGTGCCCAC GCAGCCAGGG 
GACGCGGCCG CCACATCTGC TGCTCCTGCC GACGTAGACG CAGCCAAGGA GAAGGCCAAG 
CGCGAGCAGG AGGACGCGGC GCTCGCACGC GCCATCGCCA TCGCCGAGGG CTGGGGCGAG 
GAGGAGTATG GGGAGGAGTA CGGTGAGGAG GATGAGGAGG ATGACGACGA TGAGGACGAG 
GAGGAGGAGG CGGTGGACGA GGACGACGAG GACTATGAGG GCGCCCGGCG GCGCAAGCGG 
CCGAAGGCCG CCGTCGCGCC GCCGCCGCGG ACGCATTTTA GCATGGCGCC GCCGCCAATG 
CCGTCGCCCG TGCCCCGGCC GGCTGGCGCC CCTGCGGGTG CTGCGCCGGG GCCCGCAGGT 
GGCGGCGGCC GTATACAGAT GACGCTGCCG GTAACCATGC GCGCGTCCGC GGCTCGCAGC 
AACGCCTCGG CGCGGCGGCA GGTGTGCGGC CGGACGCTGT TCGACGGCCT GCCAGGCGGC 
CTCAAGCACG ACGACATCGT GTGGTACACG CTGCAGGGCA AGCGGCTCCT GCGCGGCGTG 
GTTCAGCTGC AGGCGTGTGG CGCCGCGACC AGCGGCATCC TGTGCGGCCA CTGCGACACC 
ATCATCAGCG CCTCCGCCTT CGAGGCGCAT GCAGGCCACA AGCACCGGCG CAACCCGTAT 
GAGGCCATCC TCACCGATGA CGGCATGACG CTGAAGCGTA TCGCCGAGCG CCTCATGCCC 
ATTGCCGCCA GCCAGGCAGG CCACGCCTCC CCGCCACAGC CGCGCGCCAA GGGCGCTGGC 
GGTGCCCTGG CGGCCGCCGC CGCCGCGGTC GCGGCCGCAG CTCACGCCGC AGCGGCAGTG 
CATCACGATG ATCACCAACA CCACCACGAC GGCGGTGAGG CCGGCGAGGA CGACGACGAC 
GTGGAGATGG TCGAGCTGGT GGATGACGCT GCCTGCGTGC TGTGCCACGA GCCGGACTTT 
GACCGGGAGG GCTTCAGCGA CAAGACCGTG CTTATCTGCG ACCAGTGCGA GAAGGAGTAC 
CACATCGGCT GCCTGCGTCA GCACAAGATG GTGGACATGC AGGCGGTGCC GGAGGGCGAA 
TGGTTCTGCA GCGACGAGTG CGTGCGCATT CGCGATGCGC TGGGCGAGGA CGTGGCGGCG 
GGCGAGGTGC TCATGCCTGG CAACCCCGCC TACCGCTGGC AGATCCTGCG CGGCAAGAAT 
GGCCGGCAGC AGACCTGGCA CGCGCTGTCC ACTGTGCTCA ACATCCTTCA GGAGTCGTTC 
GACCCCATCA TCGACACCGG CAGCGGCTCC GACCTGCTGC CGGCCATGGT CAACGCCGAG 
ACGGCGGGCG ACTACGACTT CCAGGGAATG TACTCCATCC TGCTGCGGTA CAGGGGCCCG 
GACAAGGAGG CGCGCGGCAA GCCGGTGCTG GCGGCGCTGT TCCGCGTGCT GGGCAGCAGT 
ATGGCGGAGA TGCCGCTGGT GGCCACCCGC TACGACTGCC GCCGCCAGGG CCACCTGCGC 
GCGCTGGTGG ACGCGATGCG CCACAAGCTG CTGGGGCTGG GCGTGCGCGC CATCGTTCTG 
CCCGCCACGG CCGACGCGCA ACCCGCCTGG CGCCAGCTGC AGTTCCAGGA CCTGGACGAG 
CCGTCCACAC GCGTGGCGCG CAGCGAGCAC CGCATGGTCA TCTTCCCGCA CACCACCGTG 
CTGGCGCGCC CGCTCATCCG CGTGCCTGGC TACGACCAGA GCCAGCTGCA GTCCGCGCAC 
GAGGCGGCCG ACCCAGCATT CATCGCCGCC TGCGCCAAAG CCATGGAGGA GGCCCGCGTC 
GCCTCCGGCA AGGAGATCGT GGACAGCGAG GAGAAGATCT ACGCCGCTAT GGACGACGCG 
GCGGCCGCCG CCGCGGAGGT GCAGGCGGCC AAGGCGGCCG CGCTGGCGGA GCAAGGCATT 
TTCGTAGGCC GCGGCCGAGG CCGCGGCCGC GGGCGGGGGC GAGGTCGTGG TTTTGCGGCG 
GCCGCCGCCA CCGGAGGGGG CTACGGTGCG CCATACAGCG CCGCCGGGCC TGTGGAGGTG 
GATGCCTACG GGCAGCCGGT GGAGGGCGAG CCGTACAGCG GCGCCGGAGG GCCATACCCT 
GGCGCGCCGC CGCACTTGGC GCTGCTGCCG GGCTCGCCGG GCGCACCGGG CCGGGGGCGC 
GGCCGGGGGC GCGGGCGCCG CGGGCGGCCT CCCATGTCGG CCCATGTCGC GTACCCGCAC 
CCCGGCGCTG TCGCCGGCGA CCCAGGCGCC GGCGATTATC CGGCCGCACC GCCCGAGGGT 
GATGACGGCA TGGCGGCGTA CGGCGAAGGC GCGGGCGAGG GCGATGACGG CGAGGAGGGC 
GACGACACCT GGGCCGGCTA CGGCGGCGTC GGTGAGGTGG CGTCGGCGGT GGGCAGCGCT 
GTTGCTGCAG GCGAGACGGG CGAGGGCATG ATGACGGCGG CCATGGGCGC GGCGTCACGC 
TTCTCCATGG GCCCGCTGCA AGGCGCCATG GTGGCAGCTG CGGCCGCGGC CGCGGCAGCG 
GCGGCAAAGC AGTACGCGCC GCAGATGGCG GCGGTGGATG CGGCGGGCAG GCCGCGGCAG 
CCATCCGACG GCACGGATGC CGCTGGCATG CCGGCAGCGG CCTTTGGCAT GCCGCCGCCT 
CAGCAGCCAG GCCAGGGGCT TGCAGCCTTC GGCGCGGCCG CATCTGGGGC GTCCGTAGCC 
GGCATGGCGC CCGGCTACGG TGCGGATGCT GCCGGGCAAG CTGGAGCGCA GGCGCCCGCA 
GCGGTGGCTG CGGCTGCGGC GGCTTACAAG TGGGCGCTGG AGCAGTTCAC GCAGAAGCAG 
CACCAGCAGC AACCGCCAGC ACCGGCAGCC GGCCCTGGGC CGGAAAGCAC AGGCGCCGCA 
GCGTACGGCA TGGCGGCGCG GGCTTACGGC AACGCGATGA ATGCTGGGAT GGCGGCGGCG 
ACAGCAGCGC GCACTAGTGG CGGCGGCGCC CCGCCTACCG GCGCGTGGAG CAGCCAGCCG 
CCGGGCTGGC CGCAGCCGCA GCAGGTGCAC TACCAGCCGC CGACATCCCA GCCGCCGCAG 
CCAGTGTCCT TGCAGCCGAG CCAGCAGCAG CTGCAGGCCT GCAATTTCCT TGCAAGCCTA 
ACGGGCGGCG GCGGCCTCTC TAGTGGTGGT GCGCCGCCGG GCCCTGCGGC AGGCGCGCCA 
GGGAGCAGCT GGGGAGCTCC TCCGGCGCAA GCGCCGCAGG CGGCTCAGCA TTCCTACCAG 
CCACCGCCGC AGCCGCAGCC ACCGCAGCAG CACCATCACC AACCGCACCA GCTGCACCAG 
CAGCCGCACC AGATGCAGGG CGGCATGGTG GCTTACCACC AAACGCACCA GCAACCATTG 
TCAGCGGCGC AGCCGCAGCA CTATCAGCAG CAGCCTGGCG CGTGGCCCCA AGCGGCTGCG 
GGCGGCAGCA ACCCATCATT GACAGACCAG AACTACCACG AGACGCTTGC GGCACTGCAG 
CGGCAGCAGG AGCGGCAAAA CCAACAAGCG TTGCTGGGGA TACCGCCGGC TCAGCAGCAA 
CAAGCACAGC AGCACCCGCA GTTCCCGCAG CATGGGGGTT ATCCTACCGG GATTGCGCCG 
CCGCAGCAGC CGCCGCAGCA GCAACAGCAG TATGGGTATC CTGACCCGCA GGCGGCTGCG 
CAGCAGGCGG CTGCGCAGTT CGCTGCGTAC GGGCAGTACG GGGGGCCGGC TGGGGCGCCA 
GGGCTGTTCG GCCAGCAGCC GCAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Chlamydomonas genome reveals the evolution of key animal and plant functions.
Science (New York, N.Y.)318(5848)245-50(2007 Oct)
Merchant SS,Prochnik SE,Vallon O,Harris EH,Karpowicz SJ,Witman GB,Terry A,Salamov A,Fritz-Laylin LK,Mar?chal-Drouard L,Marshall WF,Qu LH,Nelson DR,Sanderfoot AA,Spalding MH,Kapitonov VV,Ren Q,Ferris P,Lindquist E,Shapiro H,Lucas SM,Grimwood J,Schmutz J,Cardol P,Cerutti H,Chanfreau G,Chen CL,Cognat V,Croft MT,Dent R,Dutcher S,Fern?ndez E,Fukuzawa H,Gonz?lez-Ballester D,Gonz?lez-Halphen D,Hallmann A,Hanikenne M,Hippler M,Inwood W,Jabbari K,Kalanon M,Kuras R,Lefebvre PA,Lemaire SD,Lobanov AV,Lohr M,Manuell A,Meier I,Mets L,Mittag M,Mittelmeier T,Moroney JV,Moseley J,Napoli C,Nedelcu AM,Niyogi K,Novoselov SV,Paulsen IT,Pazour G,Purton S,Ral JP,Ria?o-Pach?n DM,Riekhof W,Rymarquis L,Schroda M,Stern D,Umen J,Willows R,Wilson N,Zimmer SL,Allmer J,Balk J,Bisova K,Chen CJ,Elias M,Gendler K,Hauser C,Lamb MR,Ledford H,Long JC,Minagawa J,Page MD,Pan J,Pootakham W,Roje S,Rose A,Stahlberg E,Terauchi AM,Yang P,Ball S,Bowler C,Dieckmann CL,Gladyshev VN,Green P,Jorgensen R,Mayfield S,Mueller-Roeber B,Rajamani S,Sayre RT,Brokstein P,Dubchak I,Goodstein D,Hornick L,Huang YW,Jhaveri J,Luo Y,Mart?nez D,Ngau WC,Otillar B,Poliakov A,Porter A,Szajkowski L,Werner G,Zhou K,Grigoriev IV,Rokhsar DS,Grossman AR

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CHLREDRAFT_146309 cDNA ORF clone, Chlamydomonas reinhardtii

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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