ITCH cDNA ORF clone, Cariama cristata(Red-legged seriema)

Gene
Clones
gRNAs

Summary

Gene Symbol	ITCH
Entrez Gene ID	104155367
Full Name	itchy E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Cariama cristata(Red-legged seriema)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009710302.1	XP_009708604.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1
XM_009710303.1	XP_009708605.1	E3 ubiquitin-protein ligase Itchy homolog isoform X2

Pathways from BioSystems
Gene Ontology

The following ITCH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITCH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa455443	XM_009710302.1 Latest version!	Cariama cristata itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa455444	XM_009710303.1 Latest version!	Cariama cristata itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ITCH ( XM_009710302.1 ) cDNA ORF clone, Cariama cristata(Red-legged seriema) -> XP_009708604.1 Cariama cristata itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa455443
Clone ID Related Accession (Same CDS sequence)	XM_009710302.1
Accession Version	XM_009710302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-20
Organism	Cariama cristata(Red-legged seriema)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009601070.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cariama cristata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCA GTGGGTCCAG ATCAGGACCA TCCAGTGGGT TTTCCATGAA ATCACAGCTT 
CAAATCACTG TTATTTCAGC AAAACTAAAA GAAAAAAATA AATGGTTTGG ACCTAGCCCT 
TATGTGGAAG TCTCAGTAGA TGGACAGTCA AAGAAGACAG AAAAATGCAA CAATACAAAT 
AGTCCGAAGT GGAAACAGCA TCTTACAGTA ATTGTCACTC CTGTAAGTAA ATTAAACTTT 
CGAGTCTGGA GCCATCAAAC ATTAAAATCT GATGTCCTTC TGGGAAGTGC TGCTCTGGAT 
ATTCAGGAGA CTTTGAAATC AAACAGTATG AAACTTGAGC AAGTAGTGGT GACATTGCAT 
CTTATTGGTG ACAGAGAACC AGCAGAAGTG GTGGGAGATT TGTCTGTTTG TCTAGATGGG 
ATGCAGGTAG ATCCTGAGCT CTTAACCAAT GGTGATGCCT CAAGTGCAAG AAGTCCAACA 
CAGAATGATA GCTCCAAAGT AAGGAATGAT ACACGAACTA ACTCAAATGG CCTTGAAAGC 
TGTGAAGATG CTGCTCCCGG TGAAAACAAG ACTGTTAATG GCAGTGATTC GCCATTACTC 
ACAAATGGAA ACTGCAAACC TTCTAGACCG CCCAGGCCTT CTAGACCACC ACCACCCACT 
CCCCGCAGAC CCACTTCAGT AGCTGCAAGT GTAAATGGTC CTTCATCCAC ATCCACAGAA 
AATGATGGAG CCAGTGCAGG ATCACTGGCA GGTACAGCTG CAAGTACATC TTCAGAACAG 
AGCCCTGAGG GGGCAACAGC TGCAACAACA GCTCCTGCAA CTGCTGCACT CACCACTCCT 
CCAGTATCAA GACAAGTCCA GCCAGTAAAT TCTCCACCGC AGGCACTTAC TCCAGTCAGT 
CAAGGTCCTC TTCCACCTGG TTGGGAGCAA AGAGTAGACC AACATGGTCG AGTATACTAT 
GTAGATCATG TAGAAAAAAG AACAACGTGG GATCGGCCAG AGCCTCTTCC TCCAAGCTGG 
GAGCGACGAG TTGACAACAT GGGGAGAATT TATTATGTTG ACCACTTCAC TAGAACAACA 
ACGTGGCAGA GACCAACATT AGAGTCTGTC CGAAATTATG AGCAGTGGCA GCTTCAGCGC 
AGTCAGCTTC AAGGAGCTAT GCAGCAATTT AATCAGAGGT TTATATATGG GAACCAGGAC 
TTTTCATCCA CACAGAACAA AGAGTTTGAT CCTCTTGGCC CTCTGCCACA TGGATGGGAA 
AAGAGAACTG ACAGCAATGG CAGAGTGTAT TTTGTCAATC ACAACACACG AATCACTCAG 
TGGGAAGATC CCAGGAGTCA GGGTCAATTA AATGAGAAAC CCTTACCAGA GGGCTGGGAA 
ATGCGATTTA CTGTGGATGG TATTCCATAT TTTGTGGATC ACAATAGAAG AACCACTACA 
TATATAGATC CCCGCACGGG CAAGTCTGCT CTAGACAATG GACCCCAGAT AGCTTATGTG 
CGTGATTTCA AGGCAAAGGT CCATTATTTC AGATTCTGGT GTCAGCAACT GGCAATGCCA 
CAACACATAA AGATTACAGT GAGCAGAAAA ACATTATTCG AGGACTCGTT TCAACAGATA 
ATGAGCTTCA GCCCTCAGGA TCTACGAAGA CGTTTATGGG TTATTTTCCC TGGTGAAGAA 
GGCTTAGATT ATGGAGGTGT TGCAAGGGAA TGGTTCTTTC TATTGTCACA TGAAGTTTTG 
AATCCAATGT ATTGCCTGTT TGAATATGCA GGGAAAGATA ACTACTGCTT ACAGATAAAC 
CCAGCCTCTT ATATTAATCC AGACCATCTG AAATACTTCC GATTTATTGG AAGATTCATT 
GCTATGGCTT TGTTCCATGG GAAATTCATT GACACGGGTT TCTCCCTGCC GTTCTATAAA 
CGTATCCTAA ACAAGCCAGT AGGACTCAAG GATTTAGAAT CTGTTGACCC AGAGTTTTAC 
AACTCTCTCA TCTGGGTAAA AGAGAATGAT ATTGAAGAAT GTGGCTTGGA AATGTTTTTC 
TCAGTTGATA AAGAAATTCT AGGTGAAATC AAAAGCCATG ATTTGAAGCC AAATGGTAGC 
AACATTCTGG TCACAGAAGA AAACAAAGAA GAATACATTA GGCTAGTAGC AGAATGGAGA 
CTTTCCCGAG GTGTTGAGGA GCAGACACAG GCTTTCTTTG AAGGCTTCAA TGAAATTCTG 
CCACAACAGT ATTTGCAGTA TTTTGATGCG AAGGAACTGG AGGTGCTTCT ATGTGGAATG 
CAAGAAATTG ATTTGAATGA CTGGCAAAGG CATACCATCT ACCGGCACTA TACCAGGACC 
AGCAGACAGA TAGTATGGTT CTGGCAGTTT GTGAAAGAAA TAGATAATGA GAAAAGAATG 
AGACTCTTGC AGTTTGTTAC TGGAACATGC AGATTACCAG TGGGAGGATT TGCCGACCTC 
ATGGGGAGCA ATGGACCCCA AAAATTTTGT ATTGAAAAAG TTGGAAAGGA AAACTGGTTA 
CCTAGAAGTC ATACCTGTTT CAATCGCTTA GACCTTCCAC CCTATAAGAA TTATGAGCAG 
TTGAAGGAAA AACTGTTGTT TGCAATTGAA GAAACAGAAG GATTTGGGCA AGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009708604.1
CDS	17..2653
Translation	MAGSGSRSGPSSGFSMKSQLQITVISAKLKEKNKWFGPSPYVEVSVDGQSKKTEKCNNTNSPKWKQHLTVIVTPVSKLNFRVWSHQTLKSDVLLGSAALDIQETLKSNSMKLEQVVVTLHLIGDREPAEVVGDLSVCLDGMQVDPELLTNGDASSARSPTQNDSSKVRNDTRTNSNGLESCEDAAPGENKTVNGSDSPLLTNGNCKPSRPPRPSRPPPPTPRRPTSVAASVNGPSSTSTENDGASAGSLAGTAASTSSEQSPEGATAATTAPATAALTTPPVSRQVQPVNSPPQALTPVSQGPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPSWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDFSSTQNKEFDPLGPLPHGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVHYFRFWCQQLAMPQHIKITVSRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESVDPEFYNSLIWVKENDIEECGLEMFFSVDKEILGEIKSHDLKPNGSNILVTEENKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHTIYRHYTRTSRQIVWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKNYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_009710302.1
Organism	Cariama cristata(Red-legged seriema)
Definition	Cariama cristata itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009710302.1

ORF Insert Sequence:

ATGGCTGGCA GTGGGTCCAG ATCAGGACCA TCCAGTGGGT TTTCCATGAA ATCACAGCTT 
CAAATCACTG TTATTTCAGC AAAACTAAAA GAAAAAAATA AATGGTTTGG ACCTAGCCCT 
TATGTGGAAG TCTCAGTAGA TGGACAGTCA AAGAAGACAG AAAAATGCAA CAATACAAAT 
AGTCCGAAGT GGAAACAGCA TCTTACAGTA ATTGTCACTC CTGTAAGTAA ATTAAACTTT 
CGAGTCTGGA GCCATCAAAC ATTAAAATCT GATGTCCTTC TGGGAAGTGC TGCTCTGGAT 
ATTCAGGAGA CTTTGAAATC AAACAGTATG AAACTTGAGC AAGTAGTGGT GACATTGCAT 
CTTATTGGTG ACAGAGAACC AGCAGAAGTG GTGGGAGATT TGTCTGTTTG TCTAGATGGG 
ATGCAGGTAG ATCCTGAGCT CTTAACCAAT GGTGATGCCT CAAGTGCAAG AAGTCCAACA 
CAGAATGATA GCTCCAAAGT AAGGAATGAT ACACGAACTA ACTCAAATGG CCTTGAAAGC 
TGTGAAGATG CTGCTCCCGG TGAAAACAAG ACTGTTAATG GCAGTGATTC GCCATTACTC 
ACAAATGGAA ACTGCAAACC TTCTAGACCG CCCAGGCCTT CTAGACCACC ACCACCCACT 
CCCCGCAGAC CCACTTCAGT AGCTGCAAGT GTAAATGGTC CTTCATCCAC ATCCACAGAA 
AATGATGGAG CCAGTGCAGG ATCACTGGCA GGTACAGCTG CAAGTACATC TTCAGAACAG 
AGCCCTGAGG GGGCAACAGC TGCAACAACA GCTCCTGCAA CTGCTGCACT CACCACTCCT 
CCAGTATCAA GACAAGTCCA GCCAGTAAAT TCTCCACCGC AGGCACTTAC TCCAGTCAGT 
CAAGGTCCTC TTCCACCTGG TTGGGAGCAA AGAGTAGACC AACATGGTCG AGTATACTAT 
GTAGATCATG TAGAAAAAAG AACAACGTGG GATCGGCCAG AGCCTCTTCC TCCAAGCTGG 
GAGCGACGAG TTGACAACAT GGGGAGAATT TATTATGTTG ACCACTTCAC TAGAACAACA 
ACGTGGCAGA GACCAACATT AGAGTCTGTC CGAAATTATG AGCAGTGGCA GCTTCAGCGC 
AGTCAGCTTC AAGGAGCTAT GCAGCAATTT AATCAGAGGT TTATATATGG GAACCAGGAC 
TTTTCATCCA CACAGAACAA AGAGTTTGAT CCTCTTGGCC CTCTGCCACA TGGATGGGAA 
AAGAGAACTG ACAGCAATGG CAGAGTGTAT TTTGTCAATC ACAACACACG AATCACTCAG 
TGGGAAGATC CCAGGAGTCA GGGTCAATTA AATGAGAAAC CCTTACCAGA GGGCTGGGAA 
ATGCGATTTA CTGTGGATGG TATTCCATAT TTTGTGGATC ACAATAGAAG AACCACTACA 
TATATAGATC CCCGCACGGG CAAGTCTGCT CTAGACAATG GACCCCAGAT AGCTTATGTG 
CGTGATTTCA AGGCAAAGGT CCATTATTTC AGATTCTGGT GTCAGCAACT GGCAATGCCA 
CAACACATAA AGATTACAGT GAGCAGAAAA ACATTATTCG AGGACTCGTT TCAACAGATA 
ATGAGCTTCA GCCCTCAGGA TCTACGAAGA CGTTTATGGG TTATTTTCCC TGGTGAAGAA 
GGCTTAGATT ATGGAGGTGT TGCAAGGGAA TGGTTCTTTC TATTGTCACA TGAAGTTTTG 
AATCCAATGT ATTGCCTGTT TGAATATGCA GGGAAAGATA ACTACTGCTT ACAGATAAAC 
CCAGCCTCTT ATATTAATCC AGACCATCTG AAATACTTCC GATTTATTGG AAGATTCATT 
GCTATGGCTT TGTTCCATGG GAAATTCATT GACACGGGTT TCTCCCTGCC GTTCTATAAA 
CGTATCCTAA ACAAGCCAGT AGGACTCAAG GATTTAGAAT CTGTTGACCC AGAGTTTTAC 
AACTCTCTCA TCTGGGTAAA AGAGAATGAT ATTGAAGAAT GTGGCTTGGA AATGTTTTTC 
TCAGTTGATA AAGAAATTCT AGGTGAAATC AAAAGCCATG ATTTGAAGCC AAATGGTAGC 
AACATTCTGG TCACAGAAGA AAACAAAGAA GAATACATTA GGCTAGTAGC AGAATGGAGA 
CTTTCCCGAG GTGTTGAGGA GCAGACACAG GCTTTCTTTG AAGGCTTCAA TGAAATTCTG 
CCACAACAGT ATTTGCAGTA TTTTGATGCG AAGGAACTGG AGGTGCTTCT ATGTGGAATG 
CAAGAAATTG ATTTGAATGA CTGGCAAAGG CATACCATCT ACCGGCACTA TACCAGGACC 
AGCAGACAGA TAGTATGGTT CTGGCAGTTT GTGAAAGAAA TAGATAATGA GAAAAGAATG 
AGACTCTTGC AGTTTGTTAC TGGAACATGC AGATTACCAG TGGGAGGATT TGCCGACCTC 
ATGGGGAGCA ATGGACCCCA AAAATTTTGT ATTGAAAAAG TTGGAAAGGA AAACTGGTTA 
CCTAGAAGTC ATACCTGTTT CAATCGCTTA GACCTTCCAC CCTATAAGAA TTATGAGCAG 
TTGAAGGAAA AACTGTTGTT TGCAATTGAA GAAACAGAAG GATTTGGGCA AGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_009710303.1 ) cDNA ORF clone, Cariama cristata(Red-legged seriema) -> XP_009708605.1 Cariama cristata itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa455444
Clone ID Related Accession (Same CDS sequence)	XM_009710303.1
Accession Version	XM_009710303.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-20
Organism	Cariama cristata(Red-legged seriema)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009601070.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cariama cristata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCA GTGGGTCCAG ATCAGGACCA TCCAGTGGGT TTTCCATGAA ATCACAGCTT 
CAAATCACTG TTATTTCAGC AAAACTAAAA GAAAAAAATA AATGGTTTGG ACCTAGCCCT 
TATGTGGAAG TCTCAGTAGA TGGACAGTCA AAGAAGACAG AAAAATGCAA CAATACAAAT 
AGTCCGAAGT GGAAACAGCA TCTTACAGTA ATTGTCACTC CTGTAAGTAA ATTAAACTTT 
CGAGTCTGGA GCCATCAAAC ATTAAAATCT GATGTCCTTC TGGGAAGTGC TGCTCTGGAT 
ATTCAGGAGA CTTTGAAATC AAACAGTATG AAACTTGAGC AAGTAGTGGT GACATTGCAT 
CTTATTGGTG ACAGAGAACC AGCAGAAGTG GTGGGAGATT TGTCTGTTTG TCTAGATGGG 
ATGCAGGTAG ATCCTGAGCT CTTAACCAAT GGTGATGCCT CAAGTGCAAG AAGTCCAACA 
CAGAATGATA GCTCCAAAGT AAGGAATGAT ACACGAACTA ACTCAAATGG CCTTGAAAGC 
TGTGAAGATG CTGCTCCCGG TGAAAACAAG ACTGTTAATG GCAGTGATTC GCCATTACTC 
ACAAATGGAA ACTGCAAACC TTCTAGACCG CCCAGGCCTT CTAGACCACC ACCACCCACT 
CCCCGCAGAC CCACTTCAGT AGCTGAAAAT GATGGAGCCA GTGCAGGATC ACTGGCAGGT 
ACAGCTGCAA GTACATCTTC AGAACAGAGC CCTGAGGGGG CAACAGCTGC AACAACAGCT 
CCTGCAACTG CTGCACTCAC CACTCCTCCA GTATCAAGAC AAGTCCAGCC AGTAAATTCT 
CCACCGCAGG CACTTACTCC AGTCAGTCAA GGTCCTCTTC CACCTGGTTG GGAGCAAAGA 
GTAGACCAAC ATGGTCGAGT ATACTATGTA GATCATGTAG AAAAAAGAAC AACGTGGGAT 
CGGCCAGAGC CTCTTCCTCC AAGCTGGGAG CGACGAGTTG ACAACATGGG GAGAATTTAT 
TATGTTGACC ACTTCACTAG AACAACAACG TGGCAGAGAC CAACATTAGA GTCTGTCCGA 
AATTATGAGC AGTGGCAGCT TCAGCGCAGT CAGCTTCAAG GAGCTATGCA GCAATTTAAT 
CAGAGGTTTA TATATGGGAA CCAGGACTTT TCATCCACAC AGAACAAAGA GTTTGATCCT 
CTTGGCCCTC TGCCACATGG ATGGGAAAAG AGAACTGACA GCAATGGCAG AGTGTATTTT 
GTCAATCACA ACACACGAAT CACTCAGTGG GAAGATCCCA GGAGTCAGGG TCAATTAAAT 
GAGAAACCCT TACCAGAGGG CTGGGAAATG CGATTTACTG TGGATGGTAT TCCATATTTT 
GTGGATCACA ATAGAAGAAC CACTACATAT ATAGATCCCC GCACGGGCAA GTCTGCTCTA 
GACAATGGAC CCCAGATAGC TTATGTGCGT GATTTCAAGG CAAAGGTCCA TTATTTCAGA 
TTCTGGTGTC AGCAACTGGC AATGCCACAA CACATAAAGA TTACAGTGAG CAGAAAAACA 
TTATTCGAGG ACTCGTTTCA ACAGATAATG AGCTTCAGCC CTCAGGATCT ACGAAGACGT 
TTATGGGTTA TTTTCCCTGG TGAAGAAGGC TTAGATTATG GAGGTGTTGC AAGGGAATGG 
TTCTTTCTAT TGTCACATGA AGTTTTGAAT CCAATGTATT GCCTGTTTGA ATATGCAGGG 
AAAGATAACT ACTGCTTACA GATAAACCCA GCCTCTTATA TTAATCCAGA CCATCTGAAA 
TACTTCCGAT TTATTGGAAG ATTCATTGCT ATGGCTTTGT TCCATGGGAA ATTCATTGAC 
ACGGGTTTCT CCCTGCCGTT CTATAAACGT ATCCTAAACA AGCCAGTAGG ACTCAAGGAT 
TTAGAATCTG TTGACCCAGA GTTTTACAAC TCTCTCATCT GGGTAAAAGA GAATGATATT 
GAAGAATGTG GCTTGGAAAT GTTTTTCTCA GTTGATAAAG AAATTCTAGG TGAAATCAAA 
AGCCATGATT TGAAGCCAAA TGGTAGCAAC ATTCTGGTCA CAGAAGAAAA CAAAGAAGAA 
TACATTAGGC TAGTAGCAGA ATGGAGACTT TCCCGAGGTG TTGAGGAGCA GACACAGGCT 
TTCTTTGAAG GCTTCAATGA AATTCTGCCA CAACAGTATT TGCAGTATTT TGATGCGAAG 
GAACTGGAGG TGCTTCTATG TGGAATGCAA GAAATTGATT TGAATGACTG GCAAAGGCAT 
ACCATCTACC GGCACTATAC CAGGACCAGC AGACAGATAG TATGGTTCTG GCAGTTTGTG 
AAAGAAATAG ATAATGAGAA AAGAATGAGA CTCTTGCAGT TTGTTACTGG AACATGCAGA 
TTACCAGTGG GAGGATTTGC CGACCTCATG GGGAGCAATG GACCCCAAAA ATTTTGTATT 
GAAAAAGTTG GAAAGGAAAA CTGGTTACCT AGAAGTCATA CCTGTTTCAA TCGCTTAGAC 
CTTCCACCCT ATAAGAATTA TGAGCAGTTG AAGGAAAAAC TGTTGTTTGC AATTGAAGAA 
ACAGAAGGAT TTGGGCAAGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009708605.1
CDS	17..2620
Translation	MAGSGSRSGPSSGFSMKSQLQITVISAKLKEKNKWFGPSPYVEVSVDGQSKKTEKCNNTNSPKWKQHLTVIVTPVSKLNFRVWSHQTLKSDVLLGSAALDIQETLKSNSMKLEQVVVTLHLIGDREPAEVVGDLSVCLDGMQVDPELLTNGDASSARSPTQNDSSKVRNDTRTNSNGLESCEDAAPGENKTVNGSDSPLLTNGNCKPSRPPRPSRPPPPTPRRPTSVAENDGASAGSLAGTAASTSSEQSPEGATAATTAPATAALTTPPVSRQVQPVNSPPQALTPVSQGPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPSWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDFSSTQNKEFDPLGPLPHGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVHYFRFWCQQLAMPQHIKITVSRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESVDPEFYNSLIWVKENDIEECGLEMFFSVDKEILGEIKSHDLKPNGSNILVTEENKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHTIYRHYTRTSRQIVWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKNYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_009710303.1
Organism	Cariama cristata(Red-legged seriema)
Definition	Cariama cristata itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009710303.1

ORF Insert Sequence:

ATGGCTGGCA GTGGGTCCAG ATCAGGACCA TCCAGTGGGT TTTCCATGAA ATCACAGCTT 
CAAATCACTG TTATTTCAGC AAAACTAAAA GAAAAAAATA AATGGTTTGG ACCTAGCCCT 
TATGTGGAAG TCTCAGTAGA TGGACAGTCA AAGAAGACAG AAAAATGCAA CAATACAAAT 
AGTCCGAAGT GGAAACAGCA TCTTACAGTA ATTGTCACTC CTGTAAGTAA ATTAAACTTT 
CGAGTCTGGA GCCATCAAAC ATTAAAATCT GATGTCCTTC TGGGAAGTGC TGCTCTGGAT 
ATTCAGGAGA CTTTGAAATC AAACAGTATG AAACTTGAGC AAGTAGTGGT GACATTGCAT 
CTTATTGGTG ACAGAGAACC AGCAGAAGTG GTGGGAGATT TGTCTGTTTG TCTAGATGGG 
ATGCAGGTAG ATCCTGAGCT CTTAACCAAT GGTGATGCCT CAAGTGCAAG AAGTCCAACA 
CAGAATGATA GCTCCAAAGT AAGGAATGAT ACACGAACTA ACTCAAATGG CCTTGAAAGC 
TGTGAAGATG CTGCTCCCGG TGAAAACAAG ACTGTTAATG GCAGTGATTC GCCATTACTC 
ACAAATGGAA ACTGCAAACC TTCTAGACCG CCCAGGCCTT CTAGACCACC ACCACCCACT 
CCCCGCAGAC CCACTTCAGT AGCTGAAAAT GATGGAGCCA GTGCAGGATC ACTGGCAGGT 
ACAGCTGCAA GTACATCTTC AGAACAGAGC CCTGAGGGGG CAACAGCTGC AACAACAGCT 
CCTGCAACTG CTGCACTCAC CACTCCTCCA GTATCAAGAC AAGTCCAGCC AGTAAATTCT 
CCACCGCAGG CACTTACTCC AGTCAGTCAA GGTCCTCTTC CACCTGGTTG GGAGCAAAGA 
GTAGACCAAC ATGGTCGAGT ATACTATGTA GATCATGTAG AAAAAAGAAC AACGTGGGAT 
CGGCCAGAGC CTCTTCCTCC AAGCTGGGAG CGACGAGTTG ACAACATGGG GAGAATTTAT 
TATGTTGACC ACTTCACTAG AACAACAACG TGGCAGAGAC CAACATTAGA GTCTGTCCGA 
AATTATGAGC AGTGGCAGCT TCAGCGCAGT CAGCTTCAAG GAGCTATGCA GCAATTTAAT 
CAGAGGTTTA TATATGGGAA CCAGGACTTT TCATCCACAC AGAACAAAGA GTTTGATCCT 
CTTGGCCCTC TGCCACATGG ATGGGAAAAG AGAACTGACA GCAATGGCAG AGTGTATTTT 
GTCAATCACA ACACACGAAT CACTCAGTGG GAAGATCCCA GGAGTCAGGG TCAATTAAAT 
GAGAAACCCT TACCAGAGGG CTGGGAAATG CGATTTACTG TGGATGGTAT TCCATATTTT 
GTGGATCACA ATAGAAGAAC CACTACATAT ATAGATCCCC GCACGGGCAA GTCTGCTCTA 
GACAATGGAC CCCAGATAGC TTATGTGCGT GATTTCAAGG CAAAGGTCCA TTATTTCAGA 
TTCTGGTGTC AGCAACTGGC AATGCCACAA CACATAAAGA TTACAGTGAG CAGAAAAACA 
TTATTCGAGG ACTCGTTTCA ACAGATAATG AGCTTCAGCC CTCAGGATCT ACGAAGACGT 
TTATGGGTTA TTTTCCCTGG TGAAGAAGGC TTAGATTATG GAGGTGTTGC AAGGGAATGG 
TTCTTTCTAT TGTCACATGA AGTTTTGAAT CCAATGTATT GCCTGTTTGA ATATGCAGGG 
AAAGATAACT ACTGCTTACA GATAAACCCA GCCTCTTATA TTAATCCAGA CCATCTGAAA 
TACTTCCGAT TTATTGGAAG ATTCATTGCT ATGGCTTTGT TCCATGGGAA ATTCATTGAC 
ACGGGTTTCT CCCTGCCGTT CTATAAACGT ATCCTAAACA AGCCAGTAGG ACTCAAGGAT 
TTAGAATCTG TTGACCCAGA GTTTTACAAC TCTCTCATCT GGGTAAAAGA GAATGATATT 
GAAGAATGTG GCTTGGAAAT GTTTTTCTCA GTTGATAAAG AAATTCTAGG TGAAATCAAA 
AGCCATGATT TGAAGCCAAA TGGTAGCAAC ATTCTGGTCA CAGAAGAAAA CAAAGAAGAA 
TACATTAGGC TAGTAGCAGA ATGGAGACTT TCCCGAGGTG TTGAGGAGCA GACACAGGCT 
TTCTTTGAAG GCTTCAATGA AATTCTGCCA CAACAGTATT TGCAGTATTT TGATGCGAAG 
GAACTGGAGG TGCTTCTATG TGGAATGCAA GAAATTGATT TGAATGACTG GCAAAGGCAT 
ACCATCTACC GGCACTATAC CAGGACCAGC AGACAGATAG TATGGTTCTG GCAGTTTGTG 
AAAGAAATAG ATAATGAGAA AAGAATGAGA CTCTTGCAGT TTGTTACTGG AACATGCAGA 
TTACCAGTGG GAGGATTTGC CGACCTCATG GGGAGCAATG GACCCCAAAA ATTTTGTATT 
GAAAAAGTTG GAAAGGAAAA CTGGTTACCT AGAAGTCATA CCTGTTTCAA TCGCTTAGAC 
CTTCCACCCT ATAAGAATTA TGAGCAGTTG AAGGAAAAAC TGTTGTTTGC AATTGAAGAA 
ACAGAAGGAT TTGGGCAAGA ATAG