MSTN cDNA ORF clone, Capra hircus(goat)

Gene
Clones
gRNAs

Summary

Gene Symbol	MSTN
Entrez Gene ID	100860887
Full Name	myostatin
Synonyms	GDF-8,GDF8
Gene Type	protein-coding
Organism	Capra hircus(goat)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001285737.1	NP_001272666.1	growth/differentiation factor 8 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Short-hairpin Mediated Myostatin Knockdown Resulted in Altered Expression of Myogenic Regulatory Factors with Enhanced Myoblast Proliferation in Fetal Myoblast Cells of Goats.
Kumar R, Singh SP, Mitra A
Animal biotechnology29(1)59-67(2018 Jan)

Quantitative Evaluation of Myostatin Gene in Stably Transfected Caprine Fibroblast Cells by Anti-Myostatin shRNA.
Jain SK, Jain H, Kumar D, Bedekar MK, Pandey AK, Sarkhel BC
Applied biochemistry and biotechnology177(2)486-97(2015 Sep)

Small interfering RNA (siRNA)-mediated knockdown of myostatin influences the expression of myogenic regulatory factors in caprine foetal myoblasts.
Kumar R, Singh SP, Kumari P, Mitra A
Applied biochemistry and biotechnology172(3)1714-24(2014 Feb)

Characterization of 5' upstream region and investigation of TTTTA deletion in 5' UTR of myostatin (MSTN) gene in Indian goat breeds.
Singh SP, Kumar R, Kumari P, Kumar S, Mitra A
Animal biotechnology25(1)55-68(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The reduced expression of myostatin gene was achieved and measured in clonal fibroblast cells by real-time PCR.
Title: Quantitative Evaluation of Myostatin Gene in Stably Transfected Caprine Fibroblast Cells by Anti-Myostatin shRNA.

This study also suggests the importance of siRNA-mediated knockdown of MSTN as a potential alternative to increase muscle mass and meat production.
Title: Small interfering RNA (siRNA)-mediated knockdown of myostatin influences the expression of myogenic regulatory factors in caprine foetal myoblasts.

A study of the MSTN 5' upstream region and investigation of 5'UTR TTTTA deletion was carried out in seven different Indian goat breeds. An 1181 bp fragment of 5' upstream region of MSTN gene was PCR amplified, cloned, and sequenced.
Title: Characterization of 5' upstream region and investigation of TTTTA deletion in 5' UTR of myostatin (MSTN) gene in Indian goat breeds.

myostatin plays a negative role in regulating the expression of adipogenesis related genes in goat fetal fibroblasts.
Title: The effect of myostatin silencing by lentiviral-mediated RNA interference on goat fetal fibroblasts.

Polymorphisms of myostatin gene as markers associated with growth in Boer goats.
Title: Polymorphisms of myostatin gene (MSTN) in four goat breeds and their effects on Boer goat growth performance.

The following MSTN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MSTN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa335198	NM_001285737.1 Latest version!	Capra hircus myostatin (MSTN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MSTN ( NM_001285737.1 ) cDNA ORF clone, Capra hircus(goat) -> NP_001272666.1 Capra hircus myostatin (MSTN), mRNA.

Price & Availability↑

CloneID	OCa335198
Clone ID Related Accession (Same CDS sequence)	NM_001285737.1
Accession Version	NM_001285737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Capra hircus(goat)
Product	growth/differentiation factor 8 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GQ246166.1. On Oct 22, 2013 this sequence version replaced XM_005675859.1. ##Evidence-Data-START## Transcript exon combination :: GQ246166.1, AY436347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02712004 [ECO:0000348] ##Evidence-Data-END##

ATGCAAAAAC TGCAAATCTT TGTTTATATT TACCTATTTA TGCTGCTTGT TGCTGGCCCA 
GTGGATCTGA ATGAGAACAG CGAGCAGAAG GAAAATGTGG AAAAAAAGGG GCTGTGTAAT 
GCATGCTTGT GGAGACAAAA CAATAAATCC TCAAGACTAG AAGCCATAAA AATCCAAATC 
CTCAGTAAGC TTCGCCTGGA AACAGCTCCT AACATCAGCA AAGATGCTAT AAGACAACTT 
TTGCCCAAGG CTCCTCCACT CCGGGAACTG ATTGATCAGT ACGATGTCCA GAGAGATGAC 
AGCAGCGACG GCTCCTTGGA AGACGATGAC TACCACGTTA CGACGGAAAC GGTCATTACC 
ATGCCCACGG AGTCTGATCT TCTAGCAGAA GTGCAAGAAA AACCCAAATG TTGCTTCTTT 
AAATTTAGCT CTAAGATACA ACACAATAAA GTAGTAAAGG CCCAACTGTG GATATATCTG 
AGACCTGTCA AGACTCCTAC AACAGTGTTT GTGCAAATCC TGAGACTCAT CAAACCCATG 
AAAGACGGTA CAAGGTATAC TGGAATCCGA TCTCTGAAAC TTGACATGAA CCCAGGCACT 
GGTATTTGGC AGAGCATTGA TGTGAAGACA GTGTTGCAAA ACTGGCTCAA ACAACCTGAA 
TCCAACTTAG GCATTGAAAT CAAAGCTTTA GATGAGAATG GTCATGATCT TGCTGTAACC 
TTCCCAGAAC CAGGAGAAGA AGGACTGAAT CCTTTTTTAG AAGTCAAGGT AACAGACACA 
CCAAAAAGAT CTAGGAGAGA TTTTGGGCTT GATTGTGATG AGCACTCCAC AGAATCTCGA 
TGCTGTCGTT ACCCTCTAAC TGTGGATTTT GAAGCCTTTG GATGGGATTG GATTATTGCA 
CCTAAAAGAT ATAAGGCCAA TTACTGCTCT GGAGAATGTG AATTTTTATT TTTGCAAAAG 
TATCCTCATA CCCATCTTGT GCACCAAGCA AACCCCAAAG GTTCAGCCGG CCCTTGCTGT 
ACTCCTACAA AGATGTCTCC AATTAATATG CTATATTTTA ATGGCAAAGA ACAAATAATA 
TATGGGAAGA TTCCAGGCAT GGTAGTAGAT CGCTGTGGGT GCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272666.1
CDS	127..1254
Translation	MQKLQIFVYIYLFMLLVAGPVDLNENSEQKENVEKKGLCNACLWRQNNKSSRLEAIKIQILSKLRLETAPNISKDAIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHVTTETVITMPTESDLLAEVQEKPKCCFFKFSSKIQHNKVVKAQLWIYLRPVKTPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNWLKQPESNLGIEIKALDENGHDLAVTFPEPGEEGLNPFLEVKVTDTPKRSRRDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFLFLQKYPHTHLVHQANPKGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIPGMVVDRCGCS

Target ORF information:

RefSeq Version	NM_001285737.1
Organism	Capra hircus(goat)
Definition	Capra hircus myostatin (MSTN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001285737.1

ORF Insert Sequence:

ATGCAAAAAC TGCAAATCTT TGTTTATATT TACCTATTTA TGCTGCTTGT TGCTGGCCCA 
GTGGATCTGA ATGAGAACAG CGAGCAGAAG GAAAATGTGG AAAAAAAGGG GCTGTGTAAT 
GCATGCTTGT GGAGACAAAA CAATAAATCC TCAAGACTAG AAGCCATAAA AATCCAAATC 
CTCAGTAAGC TTCGCCTGGA AACAGCTCCT AACATCAGCA AAGATGCTAT AAGACAACTT 
TTGCCCAAGG CTCCTCCACT CCGGGAACTG ATTGATCAGT ACGATGTCCA GAGAGATGAC 
AGCAGCGACG GCTCCTTGGA AGACGATGAC TACCACGTTA CGACGGAAAC GGTCATTACC 
ATGCCCACGG AGTCTGATCT TCTAGCAGAA GTGCAAGAAA AACCCAAATG TTGCTTCTTT 
AAATTTAGCT CTAAGATACA ACACAATAAA GTAGTAAAGG CCCAACTGTG GATATATCTG 
AGACCTGTCA AGACTCCTAC AACAGTGTTT GTGCAAATCC TGAGACTCAT CAAACCCATG 
AAAGACGGTA CAAGGTATAC TGGAATCCGA TCTCTGAAAC TTGACATGAA CCCAGGCACT 
GGTATTTGGC AGAGCATTGA TGTGAAGACA GTGTTGCAAA ACTGGCTCAA ACAACCTGAA 
TCCAACTTAG GCATTGAAAT CAAAGCTTTA GATGAGAATG GTCATGATCT TGCTGTAACC 
TTCCCAGAAC CAGGAGAAGA AGGACTGAAT CCTTTTTTAG AAGTCAAGGT AACAGACACA 
CCAAAAAGAT CTAGGAGAGA TTTTGGGCTT GATTGTGATG AGCACTCCAC AGAATCTCGA 
TGCTGTCGTT ACCCTCTAAC TGTGGATTTT GAAGCCTTTG GATGGGATTG GATTATTGCA 
CCTAAAAGAT ATAAGGCCAA TTACTGCTCT GGAGAATGTG AATTTTTATT TTTGCAAAAG 
TATCCTCATA CCCATCTTGT GCACCAAGCA AACCCCAAAG GTTCAGCCGG CCCTTGCTGT 
ACTCCTACAA AGATGTCTCC AATTAATATG CTATATTTTA ATGGCAAAGA ACAAATAATA 
TATGGGAAGA TTCCAGGCAT GGTAGTAGAT CGCTGTGGGT GCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Short-hairpin Mediated Myostatin Knockdown Resulted in Altered Expression of Myogenic Regulatory Factors with Enhanced Myoblast Proliferation in Fetal Myoblast Cells of Goats.
Animal biotechnology29(1)59-67(2018 Jan)
Kumar R,Singh SP,Mitra A

Quantitative Evaluation of Myostatin Gene in Stably Transfected Caprine Fibroblast Cells by Anti-Myostatin shRNA.
Applied biochemistry and biotechnology177(2)486-97(2015 Sep)
Jain SK,Jain H,Kumar D,Bedekar MK,Pandey AK,Sarkhel BC

Small interfering RNA (siRNA)-mediated knockdown of myostatin influences the expression of myogenic regulatory factors in caprine foetal myoblasts.
Applied biochemistry and biotechnology172(3)1714-24(2014 Feb)
Kumar R,Singh SP,Kumari P,Mitra A

Characterization of 5' upstream region and investigation of TTTTA deletion in 5' UTR of myostatin (MSTN) gene in Indian goat breeds.
Animal biotechnology25(1)55-68(2014)
Singh SP,Kumar R,Kumari P,Kumar S,Mitra A

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MSTN cDNA ORF clone, Capra hircus(goat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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