HACD1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	HACD1
Entrez Gene ID	574011
Full Name	3-hydroxyacyl-CoA dehydratase 1
Synonyms	PTPLA
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001025269.1	NP_001020440.1	very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PTPLA	NP_001020440.1
Gallus gallus (chicken)	PTPLA	XP_418620.2
Danio rerio (zebrafish)	LOC553326	XP_699783.2
Homo sapiens (human)	PTPLA	NP_055056.3
Mus musculus (house mouse)	Ptpla	NP_038963.3
Rattus norvegicus (Norway rat)	Ptpla	NP_001094471.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003815	XP_310376.5
Pan troglodytes (chimpanzee)	PTPLA	XP_003312513.1
Bos taurus (cattle)	PTPLA	XP_002692150.1
Saccharomyces cerevisiae (baker's yeast)	PHS1	NP_012438.1
Schizosaccharomyces pombe (fission yeast)	SPBC19C2.15c	NP_595700.1
Magnaporthe oryzae (rice blast fungus)	MGG_07602	XP_003711520.1
Xenopus tropicalis (tropical clawed frog)	ptpla	XP_002939049.2
	PTPLA	XP_001091308.1
Kluyveromyces lactis	KLLA0F14146g	XP_455715.1
Oryza sativa (rice)	Os01g0150800	NP_001042035.1
Oryza sativa (rice)	Os01g0150500	NP_001042034.2

Gene Ontology
Bibliography

Related articles in PubMed

Progressive Structural Defects in Canine Centronuclear Myopathy Indicate a Role for HACD1 in Maintaining Skeletal Muscle Membrane Systems.
Walmsley GL, Blot S, Venner K, Sewry C, Laporte J, Blondelle J, Barth?l?my I, Maurer M, Blanchard-Gutton N, Pilot-Storck F, Tiret L, Piercy RJ
The American journal of pathology187(2)441-456(2017 Feb)

HACD1, a regulator of membrane composition and fluidity, promotes myoblast fusion and skeletal muscle growth.
Blondelle J, Ohno Y, Gache V, Guyot S, Storck S, Blanchard-Gutton N, Barth?l?my I, Walmsley G, Rahier A, Gadin S, Maurer M, Guillaud L, Prola A, Ferry A, Aubin-Houzelstein G, Demarquoy J, Relaix F, Piercy RJ, Blot S, Kihara A, Tiret L, Pilot-Storck F
Journal of molecular cell biology7(5)429-40(2015 Oct)

Centronuclear myopathy in Labrador retrievers: a recent founder mutation in the PTPLA gene has rapidly disseminated worldwide.
Maurer M, Mary J, Guillaud L, Fender M, Pel? M, Bilzer T, Olby N, Penderis J, Shelton GD, Panthier JJ, Thibaud JL, Barth?l?my I, Aubin-Houzelstein G, Blot S, Hitte C, Tiret L
PloS one7(10)e46408(2012)

SINE exonic insertion in the PTPLA gene leads to multiple splicing defects and segregates with the autosomal recessive centronuclear myopathy in dogs.
Pel? M, Tiret L, Kessler JL, Blot S, Panthier JJ
Human molecular genetics14(11)1417-27(2005 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

confirm that dogs deficient in HACD1 are relevant models
Title: Progressive Structural Defects in Canine Centronuclear Myopathy Indicate a Role for HACD1 in Maintaining Skeletal Muscle Membrane Systems.

Loss of function in myoblasts, knockout mice and spontaneously affected Labrador retrievers leads to reduced myoblast fusion (hypotrophy), associated with modified lipid composition and physical properties of membranes.
Title: HACD1, a regulator of membrane composition and fluidity, promotes myoblast fusion and skeletal muscle growth.

Labrador retrievers carrying two copies of a unique HACD1 loss-of-function allele that recently disseminated worldwide, are all affected by a congenital myopathy, confirming its role in muscle development.
Title: Centronuclear myopathy in Labrador retrievers: a recent founder mutation in the PTPLA gene has rapidly disseminated worldwide.

A loss-of-function mutation identified by genetic mapping in a French pedigree of Labrador retrievers causes a congenital myopathy named centronuclear myopathy (CNM).
Title: SINE exonic insertion in the PTPLA gene leads to multiple splicing defects and segregates with the autosomal recessive centronuclear myopathy in dogs.

The following HACD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HACD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00213	NM_001025269.1 Latest version!	Canis lupus familiaris 3-hydroxyacyl-CoA dehydratase 1 (HACD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HACD1 ( NM_001025269.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001020440.1 Canis lupus familiaris 3-hydroxyacyl-CoA dehydratase 1 (HACD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OCe00213
Clone ID Related Accession (Same CDS sequence)	NM_001025269.1
Accession Version	NM_001025269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Canis lupus familiaris(dog)
Product	very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ876904.1. On or before Jul 26, 2007 this sequence version replaced XM_843275.1, XM_850889.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the supported protein. ##Evidence-Data-START## Transcript exon combination :: AJ876904.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00013596, SAMN00013600 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCCA GCGAGGAGGA CGGCACCAAC GGCGGCGCCT CGGAGGCCGG CGAGGAGAAG 
GAGGCCCCCG GCAGGCGGAG GCGCCTCGGC TTGTTGGCCA CCGTCTGGCT CACCTTCTAC 
AACATCGCCA TGACCGCGGG GTGGTTGGTT CTAGCTATTG CCATGGTACG TTTTTATATG 
GAAAAAGGAA CACACAAAGG TTTATATAAA AGTATTCAGA AGACACTTAA ATTTTTCCAG 
ACATTTGCCT TGCTTGAGAT AGTCCACTGT TTAATTGGGA TTGTACCTAC TTCTGTGATT 
GTGGCTGGGG TCCAAGTGAG CTCAAGAATC TTTATGGTGT GGCTCATTAC TCACAGTATA 
AAACCAATCC AGAATGAGGA GAGCGTGGTG CTTTTTCTGG TCGCGTGGAC TGTGACAGAG 
ATCACTCGCT ATTCCTTCTA CACATTCAGT CTTCTCGACC ACTTGCCATA CTTCATTAAA 
TGGGCCAGAT ACAATTTTTT TATCATCTTA TATCCTGTCG GGGTTGTTGG TGAACTCCTT 
ACAATATATG CTGCCTTACC ATATGTGAAG AAGACAGGAA TGTTTTCGAT AAGACTTCCT 
AATAAATACA ATGTCTCCTT TGACTACTAC TATTTTCTTC TTATAACCAT GGCCTCATAT 
ATACCATTGT TCCCACAACT CTACTTTCAT ATGTTACGCC AAAGAAGAAA GGTGCTTCAT 
GGAGAAGTGA TTGTAGAAAA GGATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020440.1
CDS	42..791
Translation	MASSEEDGTNGGASEAGEEKEAPGRRRRLGLLATVWLTFYNIAMTAGWLVLAIAMVRFYMEKGTHKGLYKSIQKTLKFFQTFALLEIVHCLIGIVPTSVIVAGVQVSSRIFMVWLITHSIKPIQNEESVVLFLVAWTVTEITRYSFYTFSLLDHLPYFIKWARYNFFIILYPVGVVGELLTIYAALPYVKKTGMFSIRLPNKYNVSFDYYYFLLITMASYIPLFPQLYFHMLRQRRKVLHGEVIVEKDD

Target ORF information:

RefSeq Version	NM_001025269.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris 3-hydroxyacyl-CoA dehydratase 1 (HACD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025269.1

ORF Insert Sequence:

ATGGCGTCCA GCGAGGAGGA CGGCACCAAC GGCGGCGCCT CGGAGGCCGG CGAGGAGAAG 
GAGGCCCCCG GCAGGCGGAG GCGCCTCGGC TTGTTGGCCA CCGTCTGGCT CACCTTCTAC 
AACATCGCCA TGACCGCGGG GTGGTTGGTT CTAGCTATTG CCATGGTACG TTTTTATATG 
GAAAAAGGAA CACACAAAGG TTTATATAAA AGTATTCAGA AGACACTTAA ATTTTTCCAG 
ACATTTGCCT TGCTTGAGAT AGTCCACTGT TTAATTGGGA TTGTACCTAC TTCTGTGATT 
GTGGCTGGGG TCCAAGTGAG CTCAAGAATC TTTATGGTGT GGCTCATTAC TCACAGTATA 
AAACCAATCC AGAATGAGGA GAGCGTGGTG CTTTTTCTGG TCGCGTGGAC TGTGACAGAG 
ATCACTCGCT ATTCCTTCTA CACATTCAGT CTTCTCGACC ACTTGCCATA CTTCATTAAA 
TGGGCCAGAT ACAATTTTTT TATCATCTTA TATCCTGTCG GGGTTGTTGG TGAACTCCTT 
ACAATATATG CTGCCTTACC ATATGTGAAG AAGACAGGAA TGTTTTCGAT AAGACTTCCT 
AATAAATACA ATGTCTCCTT TGACTACTAC TATTTTCTTC TTATAACCAT GGCCTCATAT 
ATACCATTGT TCCCACAACT CTACTTTCAT ATGTTACGCC AAAGAAGAAA GGTGCTTCAT 
GGAGAAGTGA TTGTAGAAAA GGATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Progressive Structural Defects in Canine Centronuclear Myopathy Indicate a Role for HACD1 in Maintaining Skeletal Muscle Membrane Systems.
The American journal of pathology187(2)441-456(2017 Feb)
Walmsley GL,Blot S,Venner K,Sewry C,Laporte J,Blondelle J,Barth?l?my I,Maurer M,Blanchard-Gutton N,Pilot-Storck F,Tiret L,Piercy RJ

HACD1, a regulator of membrane composition and fluidity, promotes myoblast fusion and skeletal muscle growth.
Journal of molecular cell biology7(5)429-40(2015 Oct)
Blondelle J,Ohno Y,Gache V,Guyot S,Storck S,Blanchard-Gutton N,Barth?l?my I,Walmsley G,Rahier A,Gadin S,Maurer M,Guillaud L,Prola A,Ferry A,Aubin-Houzelstein G,Demarquoy J,Relaix F,Piercy RJ,Blot S,Kihara A,Tiret L,Pilot-Storck F

Centronuclear myopathy in Labrador retrievers: a recent founder mutation in the PTPLA gene has rapidly disseminated worldwide.
PloS one7(10)e46408(2012)
Maurer M,Mary J,Guillaud L,Fender M,Pel? M,Bilzer T,Olby N,Penderis J,Shelton GD,Panthier JJ,Thibaud JL,Barth?l?my I,Aubin-Houzelstein G,Blot S,Hitte C,Tiret L

SINE exonic insertion in the PTPLA gene leads to multiple splicing defects and segregates with the autosomal recessive centronuclear myopathy in dogs.
Human molecular genetics14(11)1417-27(2005 Jun)
Pel? M,Tiret L,Kessler JL,Blot S,Panthier JJ

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HACD1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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