ROT3 cDNA ORF clone, Camelina sativa(false flax)

Gene
Clones
gRNAs

Summary

Gene Symbol	ROT3
Entrez Gene ID	104716496
Full Name	3-epi-6-deoxocathasterone 23-monooxygenase-like
Gene Type	protein-coding
Organism	Camelina sativa(false flax)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001302991.1	NP_001289920.1	3-epi-6-deoxocathasterone 23-monooxygenase-like

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

De novo transcriptome analysis of an imminent biofuel crop, Camelina sativa L. using Illumina GAIIX sequencing platform and identification of SSR markers.
Mudalkar S, Golla R, Ghatty S, Reddy AR
Plant molecular biology84(1-2)159-71(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ROT3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROT3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa83027	NM_001302991.1 Latest version!	Camelina sativa 3-epi-6-deoxocathasterone 23-monooxygenase-like (ROT3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ROT3 ( NM_001302991.1 ) cDNA ORF clone, Camelina sativa(false flax) -> NP_001289920.1 Camelina sativa 3-epi-6-deoxocathasterone 23-monooxygenase-like (ROT3), mRNA.

Price & Availability↑

CloneID	OCa83027
Clone ID Related Accession (Same CDS sequence)	NM_001302991.1
Accession Version	NM_001302991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-25
Organism	Camelina sativa(false flax)
Product	3-epi-6-deoxocathasterone 23-monooxygenase-like
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KJ708758.1. On Dec 9, 2014 this sequence version replaced XM_010433878.1. ##Evidence-Data-START## Transcript exon combination :: KJ708758.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01163691, SAMN02230203 [ECO:0000348] ##Evidence-Data-END##

ATGGATTATT GGGTTGCTGG TTTCTTGGTT TTGACGACCG GAATACTTCT CCGTCCATGG 
TTCTGGTTTC GTCTAAGAGA ATCTAAAAAG AAAGATGGAG GAGATGAACA AGAAGAGAAG 
AAGAAGGGAA TGATTCCAAA GGGGAGCTTA GGCTGGCCGC TGATCGGAGA AACCCTAAAC 
TTCATCGCTT GTGGTTATTC TTCTCAGCCG GTTACCTTCA TGGAAAAACG AAAATCTTTA 
TACGGAAAGG TGTTCAAGAC GAACATAATA GGGACACCAA TCATAGTATC AACTGATGCA 
GAGGTCAACA AAGTGGTGCT CCAAAACCAC GGGAACATAT TTGTCCCTGC ATACCCTAAA 
TCAATCACTG AATTACTAGG AGAAAACTCT ATTCTCAGCA TCAATGGACC TCACCAAAAA 
AGGCTTCACA CGCTCATTAG CGCCTTCCTC AGATCTCCTC ACCTTAAAGA CCGGATCACT 
CGAGAGATCG AGACCTCAGT TTGTCTCACT TTGGCTTCTT GGGCTCAACT TCCTTTGGTT 
CATGTTCAAG ATGAGATCAA AAAGATGACG TTCGAGATAT TAGTTAAAGT GCTGATGAGC 
ACAGCCCCTG GTGAAGATTT GGACATTCTC AAACTTGAGT TCGAAGAATT TATCAAGGGT 
TTGATTTGTA TCCCCATCAA ATTCCCTGGA ACTAGACTCT ATAAATCCCT AAAGGCGAAA 
GAGAGATTAA TAAAGATGGT TAAAAAAGTT GTGGAGGAGA GACAAGTGGC GGCGGGGACG 
ACGGCGTCTC CGGCGAATGA CGTGGTAGAT GTGCTTTTAA GAGATGTTAG TGACGGTGGT 
GATTCAGAGA AGCAATCTCA GCCGTCGGAT TTCGTCAGCG GAAAGATCGT AGAGATGATG 
ATACCCGGAG AGGAAACAAT GCCAACGGCG ATGACCTTGG CTGTCAAATT CCTAAGTGAC 
AATCCCGTCG CCTTAGCCAA ACTAGTGGAG GAGAATATGG AGATGAAGAG GCGGAAATTG 
GAATCAGGTG AAGTATACAG TTGGACTGAT TATATGTCTC TCTCTTTTAC TCAAAATGTG 
ATAAACGAAA CGCTGAGAAT GGCTAACATC ATTAACGGAG TGTGGAGGAA GGCTCTCAAA 
GATGTTGAAA TTAAAGGTTA CTTGATACCA AAAGGATGGG GTGTATTGGC ATCATTCATA 
GCGGTTCACA TGGATGAAGA CATTTATGAG AATCCCTATC AATTCGATCC GTGGAGATGG 
GACAGGATTA ATGGATCTGC AAACAGCAGT ATTTGGTTCA CACCCTTTGG CGGTGGGCAA 
AGGCTATGTC CTGGTTTAGA ACTGTCGAAG CTCGAAATAG CCATCTTTCT TCACCACCTC 
GTGACGCGGT ACAGTTGGAC GGCTGAGGAA GACGAGATCG TGTCATTTCC GACGGTGAAA 
ATGAAGCGGA GGCTCCCCAT CCGAGTGGCT ATGGTTCCCA TCTCAGTTGA AAATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289920.1
CDS	1..1500
Translation	MDYWVAGFLVLTTGILLRPWFWFRLRESKKKDGGDEQEEKKKGMIPKGSLGWPLIGETLNFIACGYSSQPVTFMEKRKSLYGKVFKTNIIGTPIIVSTDAEVNKVVLQNHGNIFVPAYPKSITELLGENSILSINGPHQKRLHTLISAFLRSPHLKDRITREIETSVCLTLASWAQLPLVHVQDEIKKMTFEILVKVLMSTAPGEDLDILKLEFEEFIKGLICIPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAAGTTASPANDVVDVLLRDVSDGGDSEKQSQPSDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLESGEVYSWTDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWGVLASFIAVHMDEDIYENPYQFDPWRWDRINGSANSSIWFTPFGGGQRLCPGLELSKLEIAIFLHHLVTRYSWTAEEDEIVSFPTVKMKRRLPIRVAMVPISVENH

Target ORF information:

RefSeq Version	NM_001302991.1
Organism	Camelina sativa(false flax)
Definition	Camelina sativa 3-epi-6-deoxocathasterone 23-monooxygenase-like (ROT3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302991.1

ORF Insert Sequence:

ATGGATTATT GGGTTGCTGG TTTCTTGGTT TTGACGACCG GAATACTTCT CCGTCCATGG 
TTCTGGTTTC GTCTAAGAGA ATCTAAAAAG AAAGATGGAG GAGATGAACA AGAAGAGAAG 
AAGAAGGGAA TGATTCCAAA GGGGAGCTTA GGCTGGCCGC TGATCGGAGA AACCCTAAAC 
TTCATCGCTT GTGGTTATTC TTCTCAGCCG GTTACCTTCA TGGAAAAACG AAAATCTTTA 
TACGGAAAGG TGTTCAAGAC GAACATAATA GGGACACCAA TCATAGTATC AACTGATGCA 
GAGGTCAACA AAGTGGTGCT CCAAAACCAC GGGAACATAT TTGTCCCTGC ATACCCTAAA 
TCAATCACTG AATTACTAGG AGAAAACTCT ATTCTCAGCA TCAATGGACC TCACCAAAAA 
AGGCTTCACA CGCTCATTAG CGCCTTCCTC AGATCTCCTC ACCTTAAAGA CCGGATCACT 
CGAGAGATCG AGACCTCAGT TTGTCTCACT TTGGCTTCTT GGGCTCAACT TCCTTTGGTT 
CATGTTCAAG ATGAGATCAA AAAGATGACG TTCGAGATAT TAGTTAAAGT GCTGATGAGC 
ACAGCCCCTG GTGAAGATTT GGACATTCTC AAACTTGAGT TCGAAGAATT TATCAAGGGT 
TTGATTTGTA TCCCCATCAA ATTCCCTGGA ACTAGACTCT ATAAATCCCT AAAGGCGAAA 
GAGAGATTAA TAAAGATGGT TAAAAAAGTT GTGGAGGAGA GACAAGTGGC GGCGGGGACG 
ACGGCGTCTC CGGCGAATGA CGTGGTAGAT GTGCTTTTAA GAGATGTTAG TGACGGTGGT 
GATTCAGAGA AGCAATCTCA GCCGTCGGAT TTCGTCAGCG GAAAGATCGT AGAGATGATG 
ATACCCGGAG AGGAAACAAT GCCAACGGCG ATGACCTTGG CTGTCAAATT CCTAAGTGAC 
AATCCCGTCG CCTTAGCCAA ACTAGTGGAG GAGAATATGG AGATGAAGAG GCGGAAATTG 
GAATCAGGTG AAGTATACAG TTGGACTGAT TATATGTCTC TCTCTTTTAC TCAAAATGTG 
ATAAACGAAA CGCTGAGAAT GGCTAACATC ATTAACGGAG TGTGGAGGAA GGCTCTCAAA 
GATGTTGAAA TTAAAGGTTA CTTGATACCA AAAGGATGGG GTGTATTGGC ATCATTCATA 
GCGGTTCACA TGGATGAAGA CATTTATGAG AATCCCTATC AATTCGATCC GTGGAGATGG 
GACAGGATTA ATGGATCTGC AAACAGCAGT ATTTGGTTCA CACCCTTTGG CGGTGGGCAA 
AGGCTATGTC CTGGTTTAGA ACTGTCGAAG CTCGAAATAG CCATCTTTCT TCACCACCTC 
GTGACGCGGT ACAGTTGGAC GGCTGAGGAA GACGAGATCG TGTCATTTCC GACGGTGAAA 
ATGAAGCGGA GGCTCCCCAT CCGAGTGGCT ATGGTTCCCA TCTCAGTTGA AAATCATTAA