ssl-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	ssl-1
Entrez Gene ID	190954
Full Name	Helicase ssl-1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001268252.1	NP_001255181.1	Helicase ssl-1
NM_001268251.2	NP_001255180.1	Helicase ssl-1
NM_001268253.2	NP_001255182.1	Helicase ssl-1
NM_001268250.1	NP_001255179.1	Helicase ssl-1
NM_001268251.1	NP_001255180.1	Helicase ssl-1
NM_001268253.1	NP_001255182.1	Helicase ssl-1
NM_001268250.2	NP_001255179.1	Helicase ssl-1
NM_001268252.2	NP_001255181.1	Helicase ssl-1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ssl-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ssl-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340603	NM_001268253.2 Latest version!	Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCa340602	NM_001268251.2 Latest version!	Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa340236	NM_001268250.2 Latest version!	Caenorhabditis elegans Helicase ssl-1 (ssl-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCa340604	NM_001268252.2 Latest version!	Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCa340602	NM_001268251.1	Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCa340603	NM_001268253.1	Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCa340236	NM_001268250.1	Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCa340604	NM_001268252.1	Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ssl-1 ( NM_001268253.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255182.1 Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.

Price & Availability↑

CloneID	OCa340603
Clone ID Related Accession (Same CDS sequence)	NM_001268253.2 , NM_001268253.1
Accession Version	NM_001268253.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Helicase ssl-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001268253.1. COMPLETENESS: incomplete on the 5' end.

ATGCCAATCT GGCTTCCACC ATCACCACCA GATTCGGATG CGGATTTCGA CTTGAGAATG 
GAAGATGATT GTCTCGATCT GATGTATGAA ATTGAACAAA TGAACGAGGC TCGCCTACCA 
CAAGTTTGTC ATGAAATGAG ACGTCCGTTG GCTGAAAAAC AGCAGAAACA GAACACGTTG 
AATGCGTTTA ATGACATTCT ATCGGCAAAA GAAAAGGAAT CGGTGTACGA TGCGGTCAAC 
AAGTGCCTTC AAATGCCACA ATCCGAAGCG ATCACAGCAG AATCTGCAGC GTCTCCAGCA 
TACACGGAAC ACTCATCATT CTCGATGGAT GATACAAGCC AGGATGCGAA GATTGAGCCA 
AGTTTGACTG AAAATCAACA ACCCACCACC ACCGCCACTA CTACTACTAC AGTACCCCAA 
CAACAACAAC AACAGCAGCA GCAAAAATCG TCGAAAAAGA AGAGAAATGA TAATCGAACG 
GCTCAAAATC GAACAGCTGA AAATGGTGTG AAACGAGCGA CAACTCCACC ACCATCATGG 
CGTGAAGAGC CAGATTATGA TGGAGCCGAA TGGAATATAG TTGAAGATTA TGCACTACTT 
CAAGCAGTTC AAGTCGAATT TGCAAATGCT CATTTAGTCG AAAAATCGGC GAATGAGGGA 
ATGGTGTTGA ACTGGGAATT CGTGTCGAAT GCCGTTAATA AGCAGACAAG ATTTTTCCGC 
TCGGCCCGTC AATGCTCAAT TCGATATCAA ATGTTTGTTC GGCCAAAAGA GCTCGGACAG 
TTGGTGGCTT CTGATCCGAT TTCCAAGAAA ACGATGAAAG TCGACCTATC GCATACTGAA 
TTATCTCATT TGAGAAAAGG ACGAATGACT ACGGAGAGCC AATATGCTCA TGATTATGGA 
ATATTGACTG ATAAGAAACA TGTGAATAGA TTTAAAAGTG TTCGAGTGGC GGCAACACGG 
AGACCTGTTC AGTTTTGGAG AGGCCCTAAA GCTCTAGAAT CTCGAAATCT GCAATCTCTA 
AACGGCGGAA TGCCACCACG ACACGAGTCG AGACTCGCCG AATTCGACGT AAAAACCAAT 
ATTCGCCTGG ACGCCGAGGA CATTGTCACA ATGTCCGACG AGTCGATTGT CGCCTATGAA 
GCGAGCAAGA AGAAGCTACT GGCCAGTCGT CAAACAAAAC CCTCACCACG TCAAGATGTC 
CGATTCCATA CGCTGGTTCT TCGGCCGTAT ACCGTACCTG TGACAACTGA GTACTCGGCT 
GCACCTTCTC GTCGTGAAAT GCGCATCGCT GTTCCACCGC TTCAGCCTTC GGCTTTATCT 
ACGATTTCCT CAGTTGCTGC TGCTGCCACG TCTGGGCCAC TACCATCAAT TCAGCATTTG 
CAGTCGTCGT CTACGGGCTT GGGATCTCAG CAAAATTTGC AAAATTCGCA TAATTCTGAG 
CAAAGAAATA ATGTGCAAAA TATGCATCAA AATCAATATA ATTCAAGTCA AAATCCGCCA 
ATACCTATCC GACAAATCGG AGCAGCATCA TCACACCAAC ATGATCAAGG ATCTCAGGGG 
CCTGGGGGAA AACCACAAGC CTATCACCTG GTGCAACAGG GATCACAGCA ACAGCAGCAG 
CAGCAGCAGC AGGCGACGTT ACAGCGAAGA AATGCGGCGG CGGCGGCAGG GTCGAATGTG 
CAGTTTATTC AGCAGCAGCA GCAGCAGCAG CAATCGGGTA AAAATTGCGG TCAAGGACAA 
AGCTTCGTTG TCATGGGCTC GCAGAGCTCA TCAAATGATG GACAAGGTGG AGCATCGACC 
GTCGGAGGAG GAGGAGGAGG ATCACAACAG CCTCACCAGC AGCAGCAGCA GCAGCCACAA 
CAAAGAATAC AGTACATTCC ACAAGTTACC GGTAGCGGAA ATAACGGTGG AGGTGGTGGA 
AGAGGAGGCT ACGGTAGTAC ACTGGTCATG CCAAGAGGAG GACGTGTTGT CAGGCCAGCG 
GGAACTCTAC CAGGCGGTGG ACGTCTGTAT GTCGATCATA ACCGTCATCC ATATCCAATG 
TCGTCAAATG TTGTGCCAGT ACGTGTTCTA CCAGCCACGC AACAAGGACA ACAACGAATG 
ATGACAGGAC AACGTCGTCC GGCTCCAGCG CCCGGTACTG TCGCCGCAAT GGTGTTGCCG 
AATCGAGGAG CTGGTGGAAT TCCGCAAATG CGCAGTTTGC AACGAGGCAG TTACACCGGC 
GGTGGTGGTC AGCAACGAAT CAACGTGATG GTTCAACCAC AACAAATGCG CAGCAACAAT 
GGCGGTGGAG TCGGTGGCCA AGGAGGCCTC CAGGGTGGTC CAGGAGGTCC GCAAGGAATT 
CGTCGGCCAC TCGTCGGACG GCCACTACAA CGAGGAGTCG ATAATCAGGC GCCGACGGTT 
GCTCAGGTCG TTGTTGCTCC GCCGCAAGGA ATGCAGCAGG CATCACAAGG ACCACCCGTA 
CTTCATATGC AGAGAGCGGT TTCCATGCAA ATGCCGACGA GTCATCATCA TCAAGGCCAA 
CAGCAGGCTC CTCCGCAGAG CTCACAGCAG GCTTCGCAAC AGGCTCCCAC ATCGGATTCT 
GGGACGAGTG CTCCGCCACG ACAAGCACCA CCACCACAAA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255182.1
CDS	1..2625
Translation	MPIWLPPSPPDSDADFDLRMEDDCLDLMYEIEQMNEARLPQVCHEMRRPLAEKQQKQNTLNAFNDILSAKEKESVYDAVNKCLQMPQSEAITAESAASPAYTEHSSFSMDDTSQDAKIEPSLTENQQPTTTATTTTTVPQQQQQQQQQKSSKKKRNDNRTAQNRTAENGVKRATTPPPSWREEPDYDGAEWNIVEDYALLQAVQVEFANAHLVEKSANEGMVLNWEFVSNAVNKQTRFFRSARQCSIRYQMFVRPKELGQLVASDPISKKTMKVDLSHTELSHLRKGRMTTESQYAHDYGILTDKKHVNRFKSVRVAATRRPVQFWRGPKALESRNLQSLNGGMPPRHESRLAEFDVKTNIRLDAEDIVTMSDESIVAYEASKKKLLASRQTKPSPRQDVRFHTLVLRPYTVPVTTEYSAAPSRREMRIAVPPLQPSALSTISSVAAAATSGPLPSIQHLQSSSTGLGSQQNLQNSHNSEQRNNVQNMHQNQYNSSQNPPIPIRQIGAASSHQHDQGSQGPGGKPQAYHLVQQGSQQQQQQQQQATLQRRNAAAAAGSNVQFIQQQQQQQQSGKNCGQGQSFVVMGSQSSSNDGQGGASTVGGGGGGSQQPHQQQQQQPQQRIQYIPQVTGSGNNGGGGGRGGYGSTLVMPRGGRVVRPAGTLPGGGRLYVDHNRHPYPMSSNVVPVRVLPATQQGQQRMMTGQRRPAPAPGTVAAMVLPNRGAGGIPQMRSLQRGSYTGGGGQQRINVMVQPQQMRSNNGGGVGGQGGLQGGPGGPQGIRRPLVGRPLQRGVDNQAPTVAQVVVAPPQGMQQASQGPPVLHMQRAVSMQMPTSHHHQGQQQAPPQSSQQASQQAPTSDSGTSAPPRQAPPPQN

Target ORF information:

RefSeq Version	NM_001268253.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268253.2

ORF Insert Sequence:

ATGCCAATCT GGCTTCCACC ATCACCACCA GATTCGGATG CGGATTTCGA CTTGAGAATG 
GAAGATGATT GTCTCGATCT GATGTATGAA ATTGAACAAA TGAACGAGGC TCGCCTACCA 
CAAGTTTGTC ATGAAATGAG ACGTCCGTTG GCTGAAAAAC AGCAGAAACA GAACACGTTG 
AATGCGTTTA ATGACATTCT ATCGGCAAAA GAAAAGGAAT CGGTGTACGA TGCGGTCAAC 
AAGTGCCTTC AAATGCCACA ATCCGAAGCG ATCACAGCAG AATCTGCAGC GTCTCCAGCA 
TACACGGAAC ACTCATCATT CTCGATGGAT GATACAAGCC AGGATGCGAA GATTGAGCCA 
AGTTTGACTG AAAATCAACA ACCCACCACC ACCGCCACTA CTACTACTAC AGTACCCCAA 
CAACAACAAC AACAGCAGCA GCAAAAATCG TCGAAAAAGA AGAGAAATGA TAATCGAACG 
GCTCAAAATC GAACAGCTGA AAATGGTGTG AAACGAGCGA CAACTCCACC ACCATCATGG 
CGTGAAGAGC CAGATTATGA TGGAGCCGAA TGGAATATAG TTGAAGATTA TGCACTACTT 
CAAGCAGTTC AAGTCGAATT TGCAAATGCT CATTTAGTCG AAAAATCGGC GAATGAGGGA 
ATGGTGTTGA ACTGGGAATT CGTGTCGAAT GCCGTTAATA AGCAGACAAG ATTTTTCCGC 
TCGGCCCGTC AATGCTCAAT TCGATATCAA ATGTTTGTTC GGCCAAAAGA GCTCGGACAG 
TTGGTGGCTT CTGATCCGAT TTCCAAGAAA ACGATGAAAG TCGACCTATC GCATACTGAA 
TTATCTCATT TGAGAAAAGG ACGAATGACT ACGGAGAGCC AATATGCTCA TGATTATGGA 
ATATTGACTG ATAAGAAACA TGTGAATAGA TTTAAAAGTG TTCGAGTGGC GGCAACACGG 
AGACCTGTTC AGTTTTGGAG AGGCCCTAAA GCTCTAGAAT CTCGAAATCT GCAATCTCTA 
AACGGCGGAA TGCCACCACG ACACGAGTCG AGACTCGCCG AATTCGACGT AAAAACCAAT 
ATTCGCCTGG ACGCCGAGGA CATTGTCACA ATGTCCGACG AGTCGATTGT CGCCTATGAA 
GCGAGCAAGA AGAAGCTACT GGCCAGTCGT CAAACAAAAC CCTCACCACG TCAAGATGTC 
CGATTCCATA CGCTGGTTCT TCGGCCGTAT ACCGTACCTG TGACAACTGA GTACTCGGCT 
GCACCTTCTC GTCGTGAAAT GCGCATCGCT GTTCCACCGC TTCAGCCTTC GGCTTTATCT 
ACGATTTCCT CAGTTGCTGC TGCTGCCACG TCTGGGCCAC TACCATCAAT TCAGCATTTG 
CAGTCGTCGT CTACGGGCTT GGGATCTCAG CAAAATTTGC AAAATTCGCA TAATTCTGAG 
CAAAGAAATA ATGTGCAAAA TATGCATCAA AATCAATATA ATTCAAGTCA AAATCCGCCA 
ATACCTATCC GACAAATCGG AGCAGCATCA TCACACCAAC ATGATCAAGG ATCTCAGGGG 
CCTGGGGGAA AACCACAAGC CTATCACCTG GTGCAACAGG GATCACAGCA ACAGCAGCAG 
CAGCAGCAGC AGGCGACGTT ACAGCGAAGA AATGCGGCGG CGGCGGCAGG GTCGAATGTG 
CAGTTTATTC AGCAGCAGCA GCAGCAGCAG CAATCGGGTA AAAATTGCGG TCAAGGACAA 
AGCTTCGTTG TCATGGGCTC GCAGAGCTCA TCAAATGATG GACAAGGTGG AGCATCGACC 
GTCGGAGGAG GAGGAGGAGG ATCACAACAG CCTCACCAGC AGCAGCAGCA GCAGCCACAA 
CAAAGAATAC AGTACATTCC ACAAGTTACC GGTAGCGGAA ATAACGGTGG AGGTGGTGGA 
AGAGGAGGCT ACGGTAGTAC ACTGGTCATG CCAAGAGGAG GACGTGTTGT CAGGCCAGCG 
GGAACTCTAC CAGGCGGTGG ACGTCTGTAT GTCGATCATA ACCGTCATCC ATATCCAATG 
TCGTCAAATG TTGTGCCAGT ACGTGTTCTA CCAGCCACGC AACAAGGACA ACAACGAATG 
ATGACAGGAC AACGTCGTCC GGCTCCAGCG CCCGGTACTG TCGCCGCAAT GGTGTTGCCG 
AATCGAGGAG CTGGTGGAAT TCCGCAAATG CGCAGTTTGC AACGAGGCAG TTACACCGGC 
GGTGGTGGTC AGCAACGAAT CAACGTGATG GTTCAACCAC AACAAATGCG CAGCAACAAT 
GGCGGTGGAG TCGGTGGCCA AGGAGGCCTC CAGGGTGGTC CAGGAGGTCC GCAAGGAATT 
CGTCGGCCAC TCGTCGGACG GCCACTACAA CGAGGAGTCG ATAATCAGGC GCCGACGGTT 
GCTCAGGTCG TTGTTGCTCC GCCGCAAGGA ATGCAGCAGG CATCACAAGG ACCACCCGTA 
CTTCATATGC AGAGAGCGGT TTCCATGCAA ATGCCGACGA GTCATCATCA TCAAGGCCAA 
CAGCAGGCTC CTCCGCAGAG CTCACAGCAG GCTTCGCAAC AGGCTCCCAC ATCGGATTCT 
GGGACGAGTG CTCCGCCACG ACAAGCACCA CCACCACAAA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssl-1 ( NM_001268251.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255180.1 Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.

Price & Availability↑

CloneID	OCa340602
Clone ID Related Accession (Same CDS sequence)	NM_001268251.2 , NM_001268251.1
Accession Version	NM_001268251.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Helicase ssl-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001268251.1. COMPLETENESS: incomplete on the 3' end.

ATGCCGGCAA CACCGGTGCG TGCTTCAAGT ACTCGAATAA GCAGACGTAC ATCATCAAGA 
TCAGTGGCTG ATGATCAGCC ATCAACTTCG TCTGCGGTGG CTCCACCTCC TTCACCCATT 
GCCATAGAAA CTGATGAAGA TGCGGTAGTT GAGGAGGAGA AAAAGAAGAA AAAGACATCA 
GATGATTTGG AAATTATCAC TCCAAGAACT CCAGTCGATC GGCGAATTCC CTACATTTGC 
TCGATTCTTT TGACTGAAAA TCGATCGATT CGCGATAAAT TGGTTCTGAG CAGCGGTCCA 
GTTCGTCAAG AAGATCACGA AGAACAGATT GCTCGAGCTC AACGGATACA GCCAGTTGTC 
GATCAAATTC AACGAGTCGA GCAAATCATA CTCAATGGTT CAGTGGAAGA TATTCTGAAA 
GATCCTCGAT TCGCAGTAAT GGCAGATCTC ACAAAAGAAC CACCACCAAC ACCTGCACCT 
CCTCCTCCAA TCCAGAAGAC AATGCAACCG ATTGAGGTGA AAATTGAGGA TTCAGAGGGC 
TCAAATACGG CTCAACCGAG TGTTCTGCCC AGTTGTGGAG GAGGAGAGAC GAATGTGGAA 
AGAGCCGCCA AAAGAGAAGC GCATGTATTG GCTCGAATCG CCGAGCTCCG TAAGAACGGC 
TTATGGTCGA ACAGTCGTCT GCCAAAGTGC GTCGAACCTG AACGTAATAA AACGCATTGG 
GATTATCTAC TGGAAGAGGT CAAATGGATG GCAGTTGATT TCCGAACCGA GACGAATACG 
AAGCGAAAAA TCGCCAAAGT TATAGCTCAC GCCATTGCGA AACAGCACCG CGACAAGCAG 
ATCGAGATTG AGAGAGCCGC CGAACGGGAG ATCAAGGAGA AGCGAAAAAT GTGTGCAGGA 
ATCGCGAAGA TGGTACGGGA TTTCTGGTCG TCTACGGATA AAGTTGTGGA TATTCGAGCG 
AAGGAAGTTC TGGAGTCGAG GCTCAGGAAG GCGAGAAATA AGCATTTGAT GTTTGTAATT 
GGACAAGTCG ATGAAATGAG CAATATTGTG CAAGAAGGAC TTGTTTCATC GTCGAAATCC 
CCATCAATTG CATCGGATCG AGATGATAAA GATGAAGAAT TCAAAGCACC TGGCTCTGAT 
TCAGAATCTG ACGATGAGCA GACAATTGCA AACGCGGAAA AGTCACAGAA AAAGGAAGAT 
GTTCGACAGG AAGTTGATGC TCTTCAAAAC GAGGCAACTG TGGATATGGA TGACTTTTTG 
TACACTTTAC CGCCGGAATA TCTGAAGGCT TATGGTCTGA CGCAGGAGGA TTTGGAGGAG 
ATGAAGCGCG AGAAATTGGA GGAGCAGAAG GCTCGGAAGG AAGCTTGTGG TGATAATGAG 
GAGAAAATGG AGATTGATGA AAGCCCATCA TCAGATGCTC AAAAGCCTTC CACCTCAAGC 
TCAGATCTCA CCGCCGAGCA GCTTCAAGAT CCAACAGCTG AAGACGGCAA CGGTGATGGT 
CATGGTGTAC TTGAAAACGT GGATTACGTG AAGCTCAACA GTCAGGATAG TGATGAACGA 
CAACAAGAGT TGGCGAATAT CGCAGAAGAA GCGCTGAAAT TCCAGCCAAA AGGATATACA 
CTTGAGACGA CACAAGTCAA GACGCCCGTA CCATTCCTGA TTCGAGGACA ACTGAGAGAA 
TATCAAATGG TTGGATTGGA TTGGATGGTT ACACTTTATG AGAAGAATTT GAATGGAATT 
CTTGCCGACG AGATGGGCCT GGGAAAGACG ATTCAAACGA TTTCCCTGCT GGCTCATATG 
GCTTGTAGTG AATCGATTTG GGGACCACAC TTGATTGTTG TGCCGACGTC TGTCATTCTG 
AATTGGGAGA TGGAGTTCAA GAAATGGTGT CCGGCTCTGA AGATTTTGAC GTATTTTGGT 
ACGGCGAAGG AGCGTGCCGA GAAGCGGAAG GGATGGATGA AGCCGAATTG TTTCCATGTG 
TGCATCACAT CATACAAGAC GGTTACTCAA GATATTAGAG CTTTTAAGCA GAGGGCCTGG 
CAGTACCTAA TTCTCGATGA AGCTCAAAAT ATCAAAAACT GGAAGTCCCA ACGTTGGCAG 
GCTCTTCTGA ATGTCCGTGC TCGACGTCGC CTTCTCCTGA CCGGAACTCC ACTTCAGAAC 
TCTCTAATGG AACTGTGGTC GTTGATGCAT TTTTTGATGC CAACAATATT CTCAAGTCAT 
GATGATTTCA AGGATTGGTT CTCGAATCCG TTGACAGGGA TGATGGAAGG AAATATGGAA 
TTCAATGCTC CACTAATCGG ACGACTTCAC AAAGTGCTCC GTCCGTTTAT TCTGCGGCGG 
CTCAAGAAGG AAGTTGAGAA GCAGCTGCCA GAGAAGACTG AGCATATTGT GAATTGTTCG 
TTGTCAAAGC GGCAGAGATA CCTGTACGAT GACTTTATGA GTCGTAGATC AACAAAGGAG 
AATCTAAAGT CTGGAAATAT GATGTCGGTG CTCAACATTG TGATGCAACT CCGAAAATGT 
TGTAATCATC CGAATCTCTT CGAGCCGCGG CCAGTTGTTG CTCCGTTCGT CGTTGAGAAG 
CTTCAGCTCG ATGTTCCGGC TCGTCTCTTT GAAATTTCGC AGCAAGATCC CTCCTCCTCC 
TCAGCTAGTC AAATTCCGGA AATTTTCAAT TTATCCAAAA TCGGCTATCA ATCTTCCGTT 
CGATCTGCAA AACCACTCAT CGAAGAGCTT GAAGCAATGA GCACTTATCC GGAGCCACGA 
GCACCAGAAG TTGGCGGATT TCGGTTCAAT CGGACGGCTT TTGTTGCAAA GAATCCGCAT 
ACGGAAGAGT CGGAGGACGA AGGTGTTATG AGAAGTCGTG TTCTGCCAAA ACCAATTAAT 
GGAACAGCTC AACCACTTCA AAATGGAAAT TCAATACCAC AAAATGCTCC AAATCGTCCA 
CAAACTTCAT GCATTCGTTC AAAAACCGTC GTAAATACAG TTCCACTGAC CATCTCCACC 
GATCGAAGTG GTTTTCATTT TAATATGGCC AATGTTGGAA GAGGTGTTGT TCGTTTGGAT 
GATTCAGCAC GTATGAGCCC ACCGCTCAAA CGTCAGAAGC TCACCGGAAC TGCAACGAAT 
TGGAGTGATT ATGTTCCGCG ACACGTTGTT GAAAAGATGG AAGAATCGAG AAAAAACCAG 
CTGGAAATTG TTCGAAGGCG ATTTGAGATG ATTCGTGCTC CGATTATTCC ACTGGAAATG 
GTTGCGCTGG TTCGAGAGGA AATTATTGCA GAATTTCCAC GTTTGGCTGT GGAAGAGGAC 
GAGGTTGTGC AGGAGAGGCT TTTGGAGTAT TGCGAGTTGT TGGTGCAAAG ATTCGGAATG 
TACGTCGAAC CAGTGCTGAC CGATGCTTGG CAGTGTCGTC CATCATCGTC TGGTCTTCCA 
TCATATATTC GCAACAATTT ATCAAATATC GAGCTGAATT CTCGTTCTCT TCTCCTCAAC 
ACCTCCACTA ATTTCGATAC CCGAATGTCG ATCTCACGTG CTCTTCAATT CCCAGAACTC 
CGTCTGATCG AGTACGATTG TGGAAAGCTT CAGACGTTGG CTGTTCTGCT TCGTCAGTTG 
TACCTGTACA AGCACAGATG TCTGATCTTC ACGCAAATGT CAAAGATGCT CGACGTTCTG 
CAGACCTTCC TTTCTCATCA CGGTTATCAG TATTTCCGCC TCGACGGTAC CACTGGTGTC 
GAACAAAGAC AGGCGATGAT GGAGCGGTTC AACGCGGATC CCAAGGTGTT TTGCTTCATT 
CTGTCGACGA GATCCGGTGG TGTTGGAGTC AATCTAACCG GTGCTGACAC TGTGATCTTC 
TACGATTCGG ATTGGAATCC GACGATGGAT GCTCAGGCTC AGGATAGATG TCATCGTATC 
GGACAGACGA GGAATGTCTC GATTTATCGA TTGATTTCCG AGCGAACAAT TGAGGAGAAT 
ATTCTGAGAA AGGCAACACA GAAGCGGCGA CTTGGAGAGT TGGCAATTGA CGAGGCTGGC 
TTCACACCCG AGTTCTTCAA ACAATCTGAC AGTATTCGGG ATCTTTTTGA TGGAGAGAAT 
GTGGAAGTGA CTGCTGTGGC AGATGTTGCG ACGACGATGA GCGAGAAAGA AATGGAGGTT 
GCGATGGCAA AGTGTGAAGA TGAAGCTGAT GTGAATGCGG CGAAGATTGC GGTGGCCGAG 
GCGAACGTTG ATAATGCGGA GTTTGATGAG AAATCATTGC CGCCGATGAG CAATTTGCAA 
GGAGATGAGG AGGCTGATGA GAAGTATATG GAGTTGATAC AACAGCTCAA ACCAATCGAA 
CGATATGCCA TTAACTTTCT TGAGACACAG TACAAGCCAG AATTTGAGGA AGAATGCAAA 
GAGGCAGAGG CTCTTATCGA CCAAAAACGC GAAGAATGGG ACAAAAATCT CAACGATACC 
GCCGTCATTG ACCTCGACGA TTCGGATAGT CTGCTGCTCA ACGATCCTTC GACTTCTGCC 
GATTTTTATC AGAGCTCAAG TCTTTTAGAC GAGGTACGCG ATCGTCGTCG TCGCAGCAGC 
AGCCTTCTCC AAAAAGCCGC TCAAAAACCG GCAAAAAAGC CTCAAAACTT CCAAATTCGT 
GCTCGCTCCC CGTCTAAGCG TAAATCTCAG GCTCCTTCCT TCGATCCATA TGTTTCGTAC 
GCACCGCACG CGCTCGCTTC TCCCCCGGAT TCCCCGCGTA AGAGAAGATC ACGTGGTGCG 
CGTAGTTTAG GTAGTGGTGG TGGTGGTGGT GGTGGTAGTA GATCTGTTGG AAGACCTGCC 
CGCCGATCAG TGAAGAAAGA AGAATCAGAT GATGATGATG AGGATTATTG CCAAGAAGAG 
GAAGTGAAGC GAAATCCGGC AGAAAAGGTC CCGCCGAAAA GAAAACGAGT TGTGTTTGTG 
GAACCTCCAG AGGTGAAGCC GCCGGAGCCG AAAAAACGAG TTGTTGTTCC TGCTCCATCA 
TCATCATCAT CAGCTCTAAC TACTCTTCCA CAACAAGGAC CGCTGATTTC GTTGCCAAAA 
GCTGTGCCAG TTGTACCTCG GCCCCAACAA CAAGCACCAC CACAGCTCAT CAAAAAGCAC 
CAGCAGACTC TGATGCCTGT GAAGGTGCTC AAGATTAGTG GTGGTGGTGG TGGTACTCCA 
GGACCATCCA GTGTATCGCC AGGTCCATCA ATCCTCCGAA GAACCGTTGT TCCAGGCATA 
GGCGCTGGTG GTGTTGGACG CCTACCGCTT GTCAGAATGC CTGTTCGCCC TCCATTTCCT 
GGCTCGCAAG CTCCTGCTCC ACCGCTGAGA AGTGGTGTTG CTCCAACAGC TCCTGCAGCA 
GCTCCACGCC AGTTCGTCGT TCCGTCGTCG AGAGTTCGAG TTATCACGAC GAGAACTCCG 
GTCGCCACCA CCATGGTGCA ACAACAACAA AGCCCGAGCC CGTTGATGTT TCCAGTCCGG 
GTTGTGCAAA GGCCCGGGCC ATCTGGACCA CCACCACCTG GACCTCCAGA TCGCCCAGGA 
TTTGGAATCT ATGAGAAGCC GAGATTCTCA CTTGGATCAC GAAGAAGCCG TGGAGATTCG 
GGCCCGGAAG ATCCGGCGCC ACCACAGCCA CCACCACCCA CCACTTCTAG GCCACCGCCA 
CAAGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255180.1
CDS	23..5671
Translation	MPATPVRASSTRISRRTSSRSVADDQPSTSSAVAPPPSPIAIETDEDAVVEEEKKKKKTSDDLEIITPRTPVDRRIPYICSILLTENRSIRDKLVLSSGPVRQEDHEEQIARAQRIQPVVDQIQRVEQIILNGSVEDILKDPRFAVMADLTKEPPPTPAPPPPIQKTMQPIEVKIEDSEGSNTAQPSVLPSCGGGETNVERAAKREAHVLARIAELRKNGLWSNSRLPKCVEPERNKTHWDYLLEEVKWMAVDFRTETNTKRKIAKVIAHAIAKQHRDKQIEIERAAEREIKEKRKMCAGIAKMVRDFWSSTDKVVDIRAKEVLESRLRKARNKHLMFVIGQVDEMSNIVQEGLVSSSKSPSIASDRDDKDEEFKAPGSDSESDDEQTIANAEKSQKKEDVRQEVDALQNEATVDMDDFLYTLPPEYLKAYGLTQEDLEEMKREKLEEQKARKEACGDNEEKMEIDESPSSDAQKPSTSSSDLTAEQLQDPTAEDGNGDGHGVLENVDYVKLNSQDSDERQQELANIAEEALKFQPKGYTLETTQVKTPVPFLIRGQLREYQMVGLDWMVTLYEKNLNGILADEMGLGKTIQTISLLAHMACSESIWGPHLIVVPTSVILNWEMEFKKWCPALKILTYFGTAKERAEKRKGWMKPNCFHVCITSYKTVTQDIRAFKQRAWQYLILDEAQNIKNWKSQRWQALLNVRARRRLLLTGTPLQNSLMELWSLMHFLMPTIFSSHDDFKDWFSNPLTGMMEGNMEFNAPLIGRLHKVLRPFILRRLKKEVEKQLPEKTEHIVNCSLSKRQRYLYDDFMSRRSTKENLKSGNMMSVLNIVMQLRKCCNHPNLFEPRPVVAPFVVEKLQLDVPARLFEISQQDPSSSSASQIPEIFNLSKIGYQSSVRSAKPLIEELEAMSTYPEPRAPEVGGFRFNRTAFVAKNPHTEESEDEGVMRSRVLPKPINGTAQPLQNGNSIPQNAPNRPQTSCIRSKTVVNTVPLTISTDRSGFHFNMANVGRGVVRLDDSARMSPPLKRQKLTGTATNWSDYVPRHVVEKMEESRKNQLEIVRRRFEMIRAPIIPLEMVALVREEIIAEFPRLAVEEDEVVQERLLEYCELLVQRFGMYVEPVLTDAWQCRPSSSGLPSYIRNNLSNIELNSRSLLLNTSTNFDTRMSISRALQFPELRLIEYDCGKLQTLAVLLRQLYLYKHRCLIFTQMSKMLDVLQTFLSHHGYQYFRLDGTTGVEQRQAMMERFNADPKVFCFILSTRSGGVGVNLTGADTVIFYDSDWNPTMDAQAQDRCHRIGQTRNVSIYRLISERTIEENILRKATQKRRLGELAIDEAGFTPEFFKQSDSIRDLFDGENVEVTAVADVATTMSEKEMEVAMAKCEDEADVNAAKIAVAEANVDNAEFDEKSLPPMSNLQGDEEADEKYMELIQQLKPIERYAINFLETQYKPEFEEECKEAEALIDQKREEWDKNLNDTAVIDLDDSDSLLLNDPSTSADFYQSSSLLDEVRDRRRRSSSLLQKAAQKPAKKPQNFQIRARSPSKRKSQAPSFDPYVSYAPHALASPPDSPRKRRSRGARSLGSGGGGGGGSRSVGRPARRSVKKEESDDDDEDYCQEEEVKRNPAEKVPPKRKRVVFVEPPEVKPPEPKKRVVVPAPSSSSSALTTLPQQGPLISLPKAVPVVPRPQQQAPPQLIKKHQQTLMPVKVLKISGGGGGTPGPSSVSPGPSILRRTVVPGIGAGGVGRLPLVRMPVRPPFPGSQAPAPPLRSGVAPTAPAAAPRQFVVPSSRVRVITTRTPVATTMVQQQQSPSPLMFPVRVVQRPGPSGPPPPGPPDRPGFGIYEKPRFSLGSRRSRGDSGPEDPAPPQPPPPTTSRPPPQA

Target ORF information:

RefSeq Version	NM_001268251.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268251.2

ORF Insert Sequence:

ATGCCGGCAA CACCGGTGCG TGCTTCAAGT ACTCGAATAA GCAGACGTAC ATCATCAAGA 
TCAGTGGCTG ATGATCAGCC ATCAACTTCG TCTGCGGTGG CTCCACCTCC TTCACCCATT 
GCCATAGAAA CTGATGAAGA TGCGGTAGTT GAGGAGGAGA AAAAGAAGAA AAAGACATCA 
GATGATTTGG AAATTATCAC TCCAAGAACT CCAGTCGATC GGCGAATTCC CTACATTTGC 
TCGATTCTTT TGACTGAAAA TCGATCGATT CGCGATAAAT TGGTTCTGAG CAGCGGTCCA 
GTTCGTCAAG AAGATCACGA AGAACAGATT GCTCGAGCTC AACGGATACA GCCAGTTGTC 
GATCAAATTC AACGAGTCGA GCAAATCATA CTCAATGGTT CAGTGGAAGA TATTCTGAAA 
GATCCTCGAT TCGCAGTAAT GGCAGATCTC ACAAAAGAAC CACCACCAAC ACCTGCACCT 
CCTCCTCCAA TCCAGAAGAC AATGCAACCG ATTGAGGTGA AAATTGAGGA TTCAGAGGGC 
TCAAATACGG CTCAACCGAG TGTTCTGCCC AGTTGTGGAG GAGGAGAGAC GAATGTGGAA 
AGAGCCGCCA AAAGAGAAGC GCATGTATTG GCTCGAATCG CCGAGCTCCG TAAGAACGGC 
TTATGGTCGA ACAGTCGTCT GCCAAAGTGC GTCGAACCTG AACGTAATAA AACGCATTGG 
GATTATCTAC TGGAAGAGGT CAAATGGATG GCAGTTGATT TCCGAACCGA GACGAATACG 
AAGCGAAAAA TCGCCAAAGT TATAGCTCAC GCCATTGCGA AACAGCACCG CGACAAGCAG 
ATCGAGATTG AGAGAGCCGC CGAACGGGAG ATCAAGGAGA AGCGAAAAAT GTGTGCAGGA 
ATCGCGAAGA TGGTACGGGA TTTCTGGTCG TCTACGGATA AAGTTGTGGA TATTCGAGCG 
AAGGAAGTTC TGGAGTCGAG GCTCAGGAAG GCGAGAAATA AGCATTTGAT GTTTGTAATT 
GGACAAGTCG ATGAAATGAG CAATATTGTG CAAGAAGGAC TTGTTTCATC GTCGAAATCC 
CCATCAATTG CATCGGATCG AGATGATAAA GATGAAGAAT TCAAAGCACC TGGCTCTGAT 
TCAGAATCTG ACGATGAGCA GACAATTGCA AACGCGGAAA AGTCACAGAA AAAGGAAGAT 
GTTCGACAGG AAGTTGATGC TCTTCAAAAC GAGGCAACTG TGGATATGGA TGACTTTTTG 
TACACTTTAC CGCCGGAATA TCTGAAGGCT TATGGTCTGA CGCAGGAGGA TTTGGAGGAG 
ATGAAGCGCG AGAAATTGGA GGAGCAGAAG GCTCGGAAGG AAGCTTGTGG TGATAATGAG 
GAGAAAATGG AGATTGATGA AAGCCCATCA TCAGATGCTC AAAAGCCTTC CACCTCAAGC 
TCAGATCTCA CCGCCGAGCA GCTTCAAGAT CCAACAGCTG AAGACGGCAA CGGTGATGGT 
CATGGTGTAC TTGAAAACGT GGATTACGTG AAGCTCAACA GTCAGGATAG TGATGAACGA 
CAACAAGAGT TGGCGAATAT CGCAGAAGAA GCGCTGAAAT TCCAGCCAAA AGGATATACA 
CTTGAGACGA CACAAGTCAA GACGCCCGTA CCATTCCTGA TTCGAGGACA ACTGAGAGAA 
TATCAAATGG TTGGATTGGA TTGGATGGTT ACACTTTATG AGAAGAATTT GAATGGAATT 
CTTGCCGACG AGATGGGCCT GGGAAAGACG ATTCAAACGA TTTCCCTGCT GGCTCATATG 
GCTTGTAGTG AATCGATTTG GGGACCACAC TTGATTGTTG TGCCGACGTC TGTCATTCTG 
AATTGGGAGA TGGAGTTCAA GAAATGGTGT CCGGCTCTGA AGATTTTGAC GTATTTTGGT 
ACGGCGAAGG AGCGTGCCGA GAAGCGGAAG GGATGGATGA AGCCGAATTG TTTCCATGTG 
TGCATCACAT CATACAAGAC GGTTACTCAA GATATTAGAG CTTTTAAGCA GAGGGCCTGG 
CAGTACCTAA TTCTCGATGA AGCTCAAAAT ATCAAAAACT GGAAGTCCCA ACGTTGGCAG 
GCTCTTCTGA ATGTCCGTGC TCGACGTCGC CTTCTCCTGA CCGGAACTCC ACTTCAGAAC 
TCTCTAATGG AACTGTGGTC GTTGATGCAT TTTTTGATGC CAACAATATT CTCAAGTCAT 
GATGATTTCA AGGATTGGTT CTCGAATCCG TTGACAGGGA TGATGGAAGG AAATATGGAA 
TTCAATGCTC CACTAATCGG ACGACTTCAC AAAGTGCTCC GTCCGTTTAT TCTGCGGCGG 
CTCAAGAAGG AAGTTGAGAA GCAGCTGCCA GAGAAGACTG AGCATATTGT GAATTGTTCG 
TTGTCAAAGC GGCAGAGATA CCTGTACGAT GACTTTATGA GTCGTAGATC AACAAAGGAG 
AATCTAAAGT CTGGAAATAT GATGTCGGTG CTCAACATTG TGATGCAACT CCGAAAATGT 
TGTAATCATC CGAATCTCTT CGAGCCGCGG CCAGTTGTTG CTCCGTTCGT CGTTGAGAAG 
CTTCAGCTCG ATGTTCCGGC TCGTCTCTTT GAAATTTCGC AGCAAGATCC CTCCTCCTCC 
TCAGCTAGTC AAATTCCGGA AATTTTCAAT TTATCCAAAA TCGGCTATCA ATCTTCCGTT 
CGATCTGCAA AACCACTCAT CGAAGAGCTT GAAGCAATGA GCACTTATCC GGAGCCACGA 
GCACCAGAAG TTGGCGGATT TCGGTTCAAT CGGACGGCTT TTGTTGCAAA GAATCCGCAT 
ACGGAAGAGT CGGAGGACGA AGGTGTTATG AGAAGTCGTG TTCTGCCAAA ACCAATTAAT 
GGAACAGCTC AACCACTTCA AAATGGAAAT TCAATACCAC AAAATGCTCC AAATCGTCCA 
CAAACTTCAT GCATTCGTTC AAAAACCGTC GTAAATACAG TTCCACTGAC CATCTCCACC 
GATCGAAGTG GTTTTCATTT TAATATGGCC AATGTTGGAA GAGGTGTTGT TCGTTTGGAT 
GATTCAGCAC GTATGAGCCC ACCGCTCAAA CGTCAGAAGC TCACCGGAAC TGCAACGAAT 
TGGAGTGATT ATGTTCCGCG ACACGTTGTT GAAAAGATGG AAGAATCGAG AAAAAACCAG 
CTGGAAATTG TTCGAAGGCG ATTTGAGATG ATTCGTGCTC CGATTATTCC ACTGGAAATG 
GTTGCGCTGG TTCGAGAGGA AATTATTGCA GAATTTCCAC GTTTGGCTGT GGAAGAGGAC 
GAGGTTGTGC AGGAGAGGCT TTTGGAGTAT TGCGAGTTGT TGGTGCAAAG ATTCGGAATG 
TACGTCGAAC CAGTGCTGAC CGATGCTTGG CAGTGTCGTC CATCATCGTC TGGTCTTCCA 
TCATATATTC GCAACAATTT ATCAAATATC GAGCTGAATT CTCGTTCTCT TCTCCTCAAC 
ACCTCCACTA ATTTCGATAC CCGAATGTCG ATCTCACGTG CTCTTCAATT CCCAGAACTC 
CGTCTGATCG AGTACGATTG TGGAAAGCTT CAGACGTTGG CTGTTCTGCT TCGTCAGTTG 
TACCTGTACA AGCACAGATG TCTGATCTTC ACGCAAATGT CAAAGATGCT CGACGTTCTG 
CAGACCTTCC TTTCTCATCA CGGTTATCAG TATTTCCGCC TCGACGGTAC CACTGGTGTC 
GAACAAAGAC AGGCGATGAT GGAGCGGTTC AACGCGGATC CCAAGGTGTT TTGCTTCATT 
CTGTCGACGA GATCCGGTGG TGTTGGAGTC AATCTAACCG GTGCTGACAC TGTGATCTTC 
TACGATTCGG ATTGGAATCC GACGATGGAT GCTCAGGCTC AGGATAGATG TCATCGTATC 
GGACAGACGA GGAATGTCTC GATTTATCGA TTGATTTCCG AGCGAACAAT TGAGGAGAAT 
ATTCTGAGAA AGGCAACACA GAAGCGGCGA CTTGGAGAGT TGGCAATTGA CGAGGCTGGC 
TTCACACCCG AGTTCTTCAA ACAATCTGAC AGTATTCGGG ATCTTTTTGA TGGAGAGAAT 
GTGGAAGTGA CTGCTGTGGC AGATGTTGCG ACGACGATGA GCGAGAAAGA AATGGAGGTT 
GCGATGGCAA AGTGTGAAGA TGAAGCTGAT GTGAATGCGG CGAAGATTGC GGTGGCCGAG 
GCGAACGTTG ATAATGCGGA GTTTGATGAG AAATCATTGC CGCCGATGAG CAATTTGCAA 
GGAGATGAGG AGGCTGATGA GAAGTATATG GAGTTGATAC AACAGCTCAA ACCAATCGAA 
CGATATGCCA TTAACTTTCT TGAGACACAG TACAAGCCAG AATTTGAGGA AGAATGCAAA 
GAGGCAGAGG CTCTTATCGA CCAAAAACGC GAAGAATGGG ACAAAAATCT CAACGATACC 
GCCGTCATTG ACCTCGACGA TTCGGATAGT CTGCTGCTCA ACGATCCTTC GACTTCTGCC 
GATTTTTATC AGAGCTCAAG TCTTTTAGAC GAGGTACGCG ATCGTCGTCG TCGCAGCAGC 
AGCCTTCTCC AAAAAGCCGC TCAAAAACCG GCAAAAAAGC CTCAAAACTT CCAAATTCGT 
GCTCGCTCCC CGTCTAAGCG TAAATCTCAG GCTCCTTCCT TCGATCCATA TGTTTCGTAC 
GCACCGCACG CGCTCGCTTC TCCCCCGGAT TCCCCGCGTA AGAGAAGATC ACGTGGTGCG 
CGTAGTTTAG GTAGTGGTGG TGGTGGTGGT GGTGGTAGTA GATCTGTTGG AAGACCTGCC 
CGCCGATCAG TGAAGAAAGA AGAATCAGAT GATGATGATG AGGATTATTG CCAAGAAGAG 
GAAGTGAAGC GAAATCCGGC AGAAAAGGTC CCGCCGAAAA GAAAACGAGT TGTGTTTGTG 
GAACCTCCAG AGGTGAAGCC GCCGGAGCCG AAAAAACGAG TTGTTGTTCC TGCTCCATCA 
TCATCATCAT CAGCTCTAAC TACTCTTCCA CAACAAGGAC CGCTGATTTC GTTGCCAAAA 
GCTGTGCCAG TTGTACCTCG GCCCCAACAA CAAGCACCAC CACAGCTCAT CAAAAAGCAC 
CAGCAGACTC TGATGCCTGT GAAGGTGCTC AAGATTAGTG GTGGTGGTGG TGGTACTCCA 
GGACCATCCA GTGTATCGCC AGGTCCATCA ATCCTCCGAA GAACCGTTGT TCCAGGCATA 
GGCGCTGGTG GTGTTGGACG CCTACCGCTT GTCAGAATGC CTGTTCGCCC TCCATTTCCT 
GGCTCGCAAG CTCCTGCTCC ACCGCTGAGA AGTGGTGTTG CTCCAACAGC TCCTGCAGCA 
GCTCCACGCC AGTTCGTCGT TCCGTCGTCG AGAGTTCGAG TTATCACGAC GAGAACTCCG 
GTCGCCACCA CCATGGTGCA ACAACAACAA AGCCCGAGCC CGTTGATGTT TCCAGTCCGG 
GTTGTGCAAA GGCCCGGGCC ATCTGGACCA CCACCACCTG GACCTCCAGA TCGCCCAGGA 
TTTGGAATCT ATGAGAAGCC GAGATTCTCA CTTGGATCAC GAAGAAGCCG TGGAGATTCG 
GGCCCGGAAG ATCCGGCGCC ACCACAGCCA CCACCACCCA CCACTTCTAG GCCACCGCCA 
CAAGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssl-1 ( NM_001268250.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255179.1 Caenorhabditis elegans Helicase ssl-1 (ssl-1), mRNA.

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CloneID	OCa340236
Clone ID Related Accession (Same CDS sequence)	NM_001268250.1 , NM_001268250.2
Accession Version	NM_001268250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Helicase ssl-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001268250.1.

ATGCCGGCAA CACCGGTGCG TGCTTCAAGT ACTCGAATAA GCAGACGTAC ATCATCAAGA 
TCAGTGGCTG ATGATCAGCC ATCAACTTCG TCTGCGGTGG CTCCACCTCC TTCACCCATT 
GCCATAGAAA CTGATGAAGA TGCGGTAGTT GAGGAGGAGA AAAAGAAGAA AAAGACATCA 
GATGATTTGG AAATTATCAC TCCAAGAACT CCAGTCGATC GGCGAATTCC CTACATTTGC 
TCGATTCTTT TGACTGAAAA TCGATCGATT CGCGATAAAT TGGTTCTGAG CAGCGGTCCA 
GTTCGTCAAG AAGATCACGA AGAACAGATT GCTCGAGCTC AACGGATACA GCCAGTTGTC 
GATCAAATTC AACGAGTCGA GCAAATCATA CTCAATGGTT CAGTGGAAGA TATTCTGAAA 
GATCCTCGAT TCGCAGTAAT GGCAGATCTC ACAAAAGAAC CACCACCAAC ACCTGCACCT 
CCTCCTCCAA TCCAGAAGAC AATGCAACCG ATTGAGGTGA AAATTGAGGA TTCAGAGGGC 
TCAAATACGG CTCAACCGAG TGTTCTGCCC AGTTGTGGAG GAGGAGAGAC GAATGTGGAA 
AGAGCCGCCA AAAGAGAAGC GCATGTATTG GCTCGAATCG CCGAGCTCCG TAAGAACGGC 
TTATGGTCGA ACAGTCGTCT GCCAAAGTGC GTCGAACCTG AACGTAATAA AACGCATTGG 
GATTATCTAC TGGAAGAGGT CAAATGGATG GCAGTTGATT TCCGAACCGA GACGAATACG 
AAGCGAAAAA TCGCCAAAGT TATAGCTCAC GCCATTGCGA AACAGCACCG CGACAAGCAG 
ATCGAGATTG AGAGAGCCGC CGAACGGGAG ATCAAGGAGA AGCGAAAAAT GTGTGCAGGA 
ATCGCGAAGA TGGTACGGGA TTTCTGGTCG TCTACGGATA AAGTTGTGGA TATTCGAGCG 
AAGGAAGTTC TGGAGTCGAG GCTCAGGAAG GCGAGAAATA AGCATTTGAT GTTTGTAATT 
GGACAAGTCG ATGAAATGAG CAATATTGTG CAAGAAGGAC TTGTTTCATC GTCGAAATCC 
CCATCAATTG CATCGGATCG AGATGATAAA GATGAAGAAT TCAAAGCACC TGGCTCTGAT 
TCAGAATCTG ACGATGAGCA GACAATTGCA AACGCGGAAA AGTCACAGAA AAAGGAAGAT 
GTTCGACAGG AAGTTGATGC TCTTCAAAAC GAGGCAACTG TGGATATGGA TGACTTTTTG 
TACACTTTAC CGCCGGAATA TCTGAAGGCT TATGGTCTGA CGCAGGAGGA TTTGGAGGAG 
ATGAAGCGCG AGAAATTGGA GGAGCAGAAG GCTCGGAAGG AAGCTTGTGG TGATAATGAG 
GAGAAAATGG AGATTGATGA AAGCCCATCA TCAGATGCTC AAAAGCCTTC CACCTCAAGC 
TCAGATCTCA CCGCCGAGCA GCTTCAAGAT CCAACAGCTG AAGACGGCAA CGGTGATGGT 
CATGGTGTAC TTGAAAACGT GGATTACGTG AAGCTCAACA GTCAGGATAG TGATGAACGA 
CAACAAGAGT TGGCGAATAT CGCAGAAGAA GCGCTGAAAT TCCAGCCAAA AGGATATACA 
CTTGAGACGA CACAAGTCAA GACGCCCGTA CCATTCCTGA TTCGAGGACA ACTGAGAGAA 
TATCAAATGG TTGGATTGGA TTGGATGGTT ACACTTTATG AGAAGAATTT GAATGGAATT 
CTTGCCGACG AGATGGGCCT GGGAAAGACG ATTCAAACGA TTTCCCTGCT GGCTCATATG 
GCTTGTAGTG AATCGATTTG GGGACCACAC TTGATTGTTG TGCCGACGTC TGTCATTCTG 
AATTGGGAGA TGGAGTTCAA GAAATGGTGT CCGGCTCTGA AGATTTTGAC GTATTTTGGT 
ACGGCGAAGG AGCGTGCCGA GAAGCGGAAG GGATGGATGA AGCCGAATTG TTTCCATGTG 
TGCATCACAT CATACAAGAC GGTTACTCAA GATATTAGAG CTTTTAAGCA GAGGGCCTGG 
CAGTACCTAA TTCTCGATGA AGCTCAAAAT ATCAAAAACT GGAAGTCCCA ACGTTGGCAG 
GCTCTTCTGA ATGTCCGTGC TCGACGTCGC CTTCTCCTGA CCGGAACTCC ACTTCAGAAC 
TCTCTAATGG AACTGTGGTC GTTGATGCAT TTTTTGATGC CAACAATATT CTCAAGTCAT 
GATGATTTCA AGGATTGGTT CTCGAATCCG TTGACAGGGA TGATGGAAGG AAATATGGAA 
TTCAATGCTC CACTAATCGG ACGACTTCAC AAAGTGCTCC GTCCGTTTAT TCTGCGGCGG 
CTCAAGAAGG AAGTTGAGAA GCAGCTGCCA GAGAAGACTG AGCATATTGT GAATTGTTCG 
TTGTCAAAGC GGCAGAGATA CCTGTACGAT GACTTTATGA GTCGTAGATC AACAAAGGAG 
AATCTAAAGT CTGGAAATAT GATGTCGGTG CTCAACATTG TGATGCAACT CCGAAAATGT 
TGTAATCATC CGAATCTCTT CGAGCCGCGG CCAGTTGTTG CTCCGTTCGT CGTTGAGAAG 
CTTCAGCTCG ATGTTCCGGC TCGTCTCTTT GAAATTTCGC AGCAAGATCC CTCCTCCTCC 
TCAGCTAGTC AAATTCCGGA AATTTTCAAT TTATCCAAAA TCGGCTATCA ATCTTCCGTT 
CGATCTGCAA AACCACTCAT CGAAGAGCTT GAAGCAATGA GCACTTATCC GGAGCCACGA 
GCACCAGAAG TTGGCGGATT TCGGTTCAAT CGGACGGCTT TTGTTGCAAA GAATCCGCAT 
ACGGAAGAGT CGGAGGACGA AGGTGTTATG AGAAGTCGTG TTCTGCCAAA ACCAATTAAT 
GGAACAGCTC AACCACTTCA AAATGGAAAT TCAATACCAC AAAATGCTCC AAATCGTCCA 
CAAACTTCAT GCATTCGTTC AAAAACCGTC GTAAATACAG TTCCACTGAC CATCTCCACC 
GATCGAAGTG GTTTTCATTT TAATATGGCC AATGTTGGAA GAGGTGTTGT TCGTTTGGAT 
GATTCAGCAC GTATGAGCCC ACCGCTCAAA CGTCAGAAGC TCACCGGAAC TGCAACGAAT 
TGGAGTGATT ATGTTCCGCG ACACGTTGTT GAAAAGATGG AAGAATCGAG AAAAAACCAG 
CTGGAAATTG TTCGAAGGCG ATTTGAGATG ATTCGTGCTC CGATTATTCC ACTGGAAATG 
GTTGCGCTGG TTCGAGAGGA AATTATTGCA GAATTTCCAC GTTTGGCTGT GGAAGAGGAC 
GAGGTTGTGC AGGAGAGGCT TTTGGAGTAT TGCGAGTTGT TGGTGCAAAG ATTCGGAATG 
TACGTCGAAC CAGTGCTGAC CGATGCTTGG CAGTGTCGTC CATCATCGTC TGGTCTTCCA 
TCATATATTC GCAACAATTT ATCAAATATC GAGCTGAATT CTCGTTCTCT TCTCCTCAAC 
ACCTCCACTA ATTTCGATAC CCGAATGTCG ATCTCACGTG CTCTTCAATT CCCAGAACTC 
CGTCTGATCG AGTACGATTG TGGAAAGCTT CAGACGTTGG CTGTTCTGCT TCGTCAGTTG 
TACCTGTACA AGCACAGATG TCTGATCTTC ACGCAAATGT CAAAGATGCT CGACGTTCTG 
CAGACCTTCC TTTCTCATCA CGGTTATCAG TATTTCCGCC TCGACGGTAC CACTGGTGTC 
GAACAAAGAC AGGCGATGAT GGAGCGGTTC AACGCGGATC CCAAGGTGTT TTGCTTCATT 
CTGTCGACGA GATCCGGTGG TGTTGGAGTC AATCTAACCG GTGCTGACAC TGTGATCTTC 
TACGATTCGG ATTGGAATCC GACGATGGAT GCTCAGGCTC AGGATAGATG TCATCGTATC 
GGACAGACGA GGAATGTCTC GATTTATCGA TTGATTTCCG AGCGAACAAT TGAGGAGAAT 
ATTCTGAGAA AGGCAACACA GAAGCGGCGA CTTGGAGAGT TGGCAATTGA CGAGGCTGGC 
TTCACACCCG AGTTCTTCAA ACAATCTGAC AGTATTCGGG ATCTTTTTGA TGGAGAGAAT 
GTGGAAGTGA CTGCTGTGGC AGATGTTGCG ACGACGATGA GCGAGAAAGA AATGGAGGTT 
GCGATGGCAA AGTGTGAAGA TGAAGCTGAT GTGAATGCGG CGAAGATTGC GGTGGCCGAG 
GCGAACGTTG ATAATGCGGA GTTTGATGAG AAATCATTGC CGCCGATGAG CAATTTGCAA 
GGAGATGAGG AGGCTGATGA GAAGTATATG GAGTTGATAC AACAGCTCAA ACCAATCGAA 
CGATATGCCA TTAACTTTCT TGAGACACAG TACAAGCCAG AATTTGAGGA AGAATGCAAA 
GAGGCAGAGG CTCTTATCGA CCAAAAACGC GAAGAATGGG ACAAAAATCT CAACGATACC 
GCCGTCATTG ACCTCGACGA TTCGGATAGT CTGCTGCTCA ACGATCCTTC GACTTCTGCC 
GATTTTTATC AGAGCTCAAG TCTTTTAGAC GAGATAAAAT TCTACGACGA GCTGGACGAT 
ATCATGCCAA TCTGGCTTCC ACCATCACCA CCAGATTCGG ATGCGGATTT CGACTTGAGA 
ATGGAAGATG ATTGTCTCGA TCTGATGTAT GAAATTGAAC AAATGAACGA GGCTCGCCTA 
CCACAAGTTT GTCATGAAAT GAGACGTCCG TTGGCTGAAA AACAGCAGAA ACAGAACACG 
TTGAATGCGT TTAATGACAT TCTATCGGCA AAAGAAAAGG AATCGGTGTA CGATGCGGTC 
AACAAGTGCC TTCAAATGCC ACAATCCGAA GCGATCACAG CAGAATCTGC AGCGTCTCCA 
GCATACACGG AACACTCATC ATTCTCGATG GATGATACAA GCCAGGATGC GAAGATTGAG 
CCAAGTTTGA CTGAAAATCA ACAACCCACC ACCACCGCCA CTACTACTAC TACAGTACCC 
CAACAACAAC AACAACAGCA GCAGCAAAAA TCGTCGAAAA AGAAGAGAAA TGATAATCGA 
ACGGCTCAAA ATCGAACAGC TGAAAATGGT GTGAAACGAG CGACAACTCC ACCACCATCA 
TGGCGTGAAG AGCCAGATTA TGATGGAGCC GAATGGAATA TAGTTGAAGA TTATGCACTA 
CTTCAAGCAG TTCAAGTCGA ATTTGCAAAT GCTCATTTAG TCGAAAAATC GGCGAATGAG 
GGAATGGTGT TGAACTGGGA ATTCGTGTCG AATGCCGTTA ATAAGCAGAC AAGATTTTTC 
CGCTCGGCCC GTCAATGCTC AATTCGATAT CAAATGTTTG TTCGGCCAAA AGAGCTCGGA 
CAGTTGGTGG CTTCTGATCC GATTTCCAAG AAAACGATGA AAGTCGACCT ATCGCATACT 
GAATTATCTC ATTTGAGAAA AGGACGAATG ACTACGGAGA GCCAATATGC TCATGATTAT 
GGAATATTGA CTGATAAGAA ACATGTGAAT AGATTTAAAA GTGTTCGAGT GGCGGCAACA 
CGGAGACCTG TTCAGTTTTG GAGAGGCCCT AAAGCTCTAG AATCTCGAAA TCTGCAATCT 
CTAAACGGCG GAATGCCACC ACGACACGAG TCGAGACTCG CCGAATTCGA CGTAAAAACC 
AATATTCGCC TGGACGCCGA GGACATTGTC ACAATGTCCG ACGAGTCGAT TGTCGCCTAT 
GAAGCGAGCA AGAAGAAGCT ACTGGCCAGT CGTCAAACAA AACCCTCACC ACGTCAAGAT 
GTCCGATTCC ATACGCTGGT TCTTCGGCCG TATACCGTAC CTGTGACAAC TGAGTACTCG 
GCTGCACCTT CTCGTCGTGA AATGCGCATC GCTGTTCCAC CGCTTCAGCC TTCGGCTTTA 
TCTACGATTT CCTCAGTTGC TGCTGCTGCC ACGTCTGGGC CACTACCATC AATTCAGCAT 
TTGCAGTCGT CGTCTACGGG CTTGGGATCT CAGCAAAATT TGCAAAATTC GCATAATTCT 
GAGCAAAGAA ATAATGTGCA AAATATGCAT CAAAATCAAT ATAATTCAAG TCAAAATCCG 
CCAATACCTA TCCGACAAAT CGGAGCAGCA TCATCACACC AACATGATCA AGGATCTCAG 
GGGCCTGGGG GAAAACCACA AGCCTATCAC CTGGTGCAAC AGGGATCACA GCAACAGCAG 
CAGCAGCAGC AGCAGGCGAC GTTACAGCGA AGAAATGCGG CGGCGGCGGC AGGGTCGAAT 
GTGCAGTTTA TTCAGCAGCA GCAGCAGCAG CAGCAATCGG GTAAAAATTG CGGTCAAGGA 
CAAAGCTTCG TTGTCATGGG CTCGCAGAGC TCATCAAATG ATGGACAAGG TGGAGCATCG 
ACCGTCGGAG GAGGAGGAGG AGGATCACAA CAGCCTCACC AGCAGCAGCA GCAGCAGCCA 
CAACAAAGAA TACAGTACAT TCCACAAGTT ACCGGTAGCG GAAATAACGG TGGAGGTGGT 
GGAAGAGGAG GCTACGGTAG TACACTGGTC ATGCCAAGAG GAGGACGTGT TGTCAGGCCA 
GCGGGAACTC TACCAGGCGG TGGACGTCTG TATGTCGATC ATAACCGTCA TCCATATCCA 
ATGTCGTCAA ATGTTGTGCC AGTACGTGTT CTACCAGCCA CGCAACAAGG ACAACAACGA 
ATGATGACAG GACAACGTCG TCCGGCTCCA GCGCCCGGTA CTGTCGCCGC AATGGTGTTG 
CCGAATCGAG GAGCTGGTGG AATTCCGCAA ATGCGCAGTT TGCAACGAGG CAGTTACACC 
GGCGGTGGTG GTCAGCAACG AATCAACGTG ATGGTTCAAC CACAACAAAT GCGCAGCAAC 
AATGGCGGTG GAGTCGGTGG CCAAGGAGGC CTCCAGGGTG GTCCAGGAGG TCCGCAAGGA 
ATTCGTCGGC CACTCGTCGG ACGGCCACTA CAACGAGGAG TCGATAATCA GGCGCCGACG 
GTTGCTCAGG TCGTTGTTGC TCCGCCGCAA GGAATGCAGC AGGCATCACA AGGACCACCC 
GTACTTCATA TGCAGAGAGC GGTTTCCATG CAAATGCCGA CGAGTCATCA TCATCAAGGC 
CAACAGCAGG CTCCTCCGCA GAGCTCACAG CAGGCTTCGC AACAGGCTCC CACATCGGAT 
TCTGGGACGA GTGCTCCGCC ACGACAAGCA CCACCACCAC AAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255179.1
CDS	23..7210
Translation	MPATPVRASSTRISRRTSSRSVADDQPSTSSAVAPPPSPIAIETDEDAVVEEEKKKKKTSDDLEIITPRTPVDRRIPYICSILLTENRSIRDKLVLSSGPVRQEDHEEQIARAQRIQPVVDQIQRVEQIILNGSVEDILKDPRFAVMADLTKEPPPTPAPPPPIQKTMQPIEVKIEDSEGSNTAQPSVLPSCGGGETNVERAAKREAHVLARIAELRKNGLWSNSRLPKCVEPERNKTHWDYLLEEVKWMAVDFRTETNTKRKIAKVIAHAIAKQHRDKQIEIERAAEREIKEKRKMCAGIAKMVRDFWSSTDKVVDIRAKEVLESRLRKARNKHLMFVIGQVDEMSNIVQEGLVSSSKSPSIASDRDDKDEEFKAPGSDSESDDEQTIANAEKSQKKEDVRQEVDALQNEATVDMDDFLYTLPPEYLKAYGLTQEDLEEMKREKLEEQKARKEACGDNEEKMEIDESPSSDAQKPSTSSSDLTAEQLQDPTAEDGNGDGHGVLENVDYVKLNSQDSDERQQELANIAEEALKFQPKGYTLETTQVKTPVPFLIRGQLREYQMVGLDWMVTLYEKNLNGILADEMGLGKTIQTISLLAHMACSESIWGPHLIVVPTSVILNWEMEFKKWCPALKILTYFGTAKERAEKRKGWMKPNCFHVCITSYKTVTQDIRAFKQRAWQYLILDEAQNIKNWKSQRWQALLNVRARRRLLLTGTPLQNSLMELWSLMHFLMPTIFSSHDDFKDWFSNPLTGMMEGNMEFNAPLIGRLHKVLRPFILRRLKKEVEKQLPEKTEHIVNCSLSKRQRYLYDDFMSRRSTKENLKSGNMMSVLNIVMQLRKCCNHPNLFEPRPVVAPFVVEKLQLDVPARLFEISQQDPSSSSASQIPEIFNLSKIGYQSSVRSAKPLIEELEAMSTYPEPRAPEVGGFRFNRTAFVAKNPHTEESEDEGVMRSRVLPKPINGTAQPLQNGNSIPQNAPNRPQTSCIRSKTVVNTVPLTISTDRSGFHFNMANVGRGVVRLDDSARMSPPLKRQKLTGTATNWSDYVPRHVVEKMEESRKNQLEIVRRRFEMIRAPIIPLEMVALVREEIIAEFPRLAVEEDEVVQERLLEYCELLVQRFGMYVEPVLTDAWQCRPSSSGLPSYIRNNLSNIELNSRSLLLNTSTNFDTRMSISRALQFPELRLIEYDCGKLQTLAVLLRQLYLYKHRCLIFTQMSKMLDVLQTFLSHHGYQYFRLDGTTGVEQRQAMMERFNADPKVFCFILSTRSGGVGVNLTGADTVIFYDSDWNPTMDAQAQDRCHRIGQTRNVSIYRLISERTIEENILRKATQKRRLGELAIDEAGFTPEFFKQSDSIRDLFDGENVEVTAVADVATTMSEKEMEVAMAKCEDEADVNAAKIAVAEANVDNAEFDEKSLPPMSNLQGDEEADEKYMELIQQLKPIERYAINFLETQYKPEFEEECKEAEALIDQKREEWDKNLNDTAVIDLDDSDSLLLNDPSTSADFYQSSSLLDEIKFYDELDDIMPIWLPPSPPDSDADFDLRMEDDCLDLMYEIEQMNEARLPQVCHEMRRPLAEKQQKQNTLNAFNDILSAKEKESVYDAVNKCLQMPQSEAITAESAASPAYTEHSSFSMDDTSQDAKIEPSLTENQQPTTTATTTTTVPQQQQQQQQQKSSKKKRNDNRTAQNRTAENGVKRATTPPPSWREEPDYDGAEWNIVEDYALLQAVQVEFANAHLVEKSANEGMVLNWEFVSNAVNKQTRFFRSARQCSIRYQMFVRPKELGQLVASDPISKKTMKVDLSHTELSHLRKGRMTTESQYAHDYGILTDKKHVNRFKSVRVAATRRPVQFWRGPKALESRNLQSLNGGMPPRHESRLAEFDVKTNIRLDAEDIVTMSDESIVAYEASKKKLLASRQTKPSPRQDVRFHTLVLRPYTVPVTTEYSAAPSRREMRIAVPPLQPSALSTISSVAAAATSGPLPSIQHLQSSSTGLGSQQNLQNSHNSEQRNNVQNMHQNQYNSSQNPPIPIRQIGAASSHQHDQGSQGPGGKPQAYHLVQQGSQQQQQQQQQATLQRRNAAAAAGSNVQFIQQQQQQQQSGKNCGQGQSFVVMGSQSSSNDGQGGASTVGGGGGGSQQPHQQQQQQPQQRIQYIPQVTGSGNNGGGGGRGGYGSTLVMPRGGRVVRPAGTLPGGGRLYVDHNRHPYPMSSNVVPVRVLPATQQGQQRMMTGQRRPAPAPGTVAAMVLPNRGAGGIPQMRSLQRGSYTGGGGQQRINVMVQPQQMRSNNGGGVGGQGGLQGGPGGPQGIRRPLVGRPLQRGVDNQAPTVAQVVVAPPQGMQQASQGPPVLHMQRAVSMQMPTSHHHQGQQQAPPQSSQQASQQAPTSDSGTSAPPRQAPPPQN

Target ORF information:

RefSeq Version	NM_001268250.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Helicase ssl-1 (ssl-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268250.2

ORF Insert Sequence:

ATGCCGGCAA CACCGGTGCG TGCTTCAAGT ACTCGAATAA GCAGACGTAC ATCATCAAGA 
TCAGTGGCTG ATGATCAGCC ATCAACTTCG TCTGCGGTGG CTCCACCTCC TTCACCCATT 
GCCATAGAAA CTGATGAAGA TGCGGTAGTT GAGGAGGAGA AAAAGAAGAA AAAGACATCA 
GATGATTTGG AAATTATCAC TCCAAGAACT CCAGTCGATC GGCGAATTCC CTACATTTGC 
TCGATTCTTT TGACTGAAAA TCGATCGATT CGCGATAAAT TGGTTCTGAG CAGCGGTCCA 
GTTCGTCAAG AAGATCACGA AGAACAGATT GCTCGAGCTC AACGGATACA GCCAGTTGTC 
GATCAAATTC AACGAGTCGA GCAAATCATA CTCAATGGTT CAGTGGAAGA TATTCTGAAA 
GATCCTCGAT TCGCAGTAAT GGCAGATCTC ACAAAAGAAC CACCACCAAC ACCTGCACCT 
CCTCCTCCAA TCCAGAAGAC AATGCAACCG ATTGAGGTGA AAATTGAGGA TTCAGAGGGC 
TCAAATACGG CTCAACCGAG TGTTCTGCCC AGTTGTGGAG GAGGAGAGAC GAATGTGGAA 
AGAGCCGCCA AAAGAGAAGC GCATGTATTG GCTCGAATCG CCGAGCTCCG TAAGAACGGC 
TTATGGTCGA ACAGTCGTCT GCCAAAGTGC GTCGAACCTG AACGTAATAA AACGCATTGG 
GATTATCTAC TGGAAGAGGT CAAATGGATG GCAGTTGATT TCCGAACCGA GACGAATACG 
AAGCGAAAAA TCGCCAAAGT TATAGCTCAC GCCATTGCGA AACAGCACCG CGACAAGCAG 
ATCGAGATTG AGAGAGCCGC CGAACGGGAG ATCAAGGAGA AGCGAAAAAT GTGTGCAGGA 
ATCGCGAAGA TGGTACGGGA TTTCTGGTCG TCTACGGATA AAGTTGTGGA TATTCGAGCG 
AAGGAAGTTC TGGAGTCGAG GCTCAGGAAG GCGAGAAATA AGCATTTGAT GTTTGTAATT 
GGACAAGTCG ATGAAATGAG CAATATTGTG CAAGAAGGAC TTGTTTCATC GTCGAAATCC 
CCATCAATTG CATCGGATCG AGATGATAAA GATGAAGAAT TCAAAGCACC TGGCTCTGAT 
TCAGAATCTG ACGATGAGCA GACAATTGCA AACGCGGAAA AGTCACAGAA AAAGGAAGAT 
GTTCGACAGG AAGTTGATGC TCTTCAAAAC GAGGCAACTG TGGATATGGA TGACTTTTTG 
TACACTTTAC CGCCGGAATA TCTGAAGGCT TATGGTCTGA CGCAGGAGGA TTTGGAGGAG 
ATGAAGCGCG AGAAATTGGA GGAGCAGAAG GCTCGGAAGG AAGCTTGTGG TGATAATGAG 
GAGAAAATGG AGATTGATGA AAGCCCATCA TCAGATGCTC AAAAGCCTTC CACCTCAAGC 
TCAGATCTCA CCGCCGAGCA GCTTCAAGAT CCAACAGCTG AAGACGGCAA CGGTGATGGT 
CATGGTGTAC TTGAAAACGT GGATTACGTG AAGCTCAACA GTCAGGATAG TGATGAACGA 
CAACAAGAGT TGGCGAATAT CGCAGAAGAA GCGCTGAAAT TCCAGCCAAA AGGATATACA 
CTTGAGACGA CACAAGTCAA GACGCCCGTA CCATTCCTGA TTCGAGGACA ACTGAGAGAA 
TATCAAATGG TTGGATTGGA TTGGATGGTT ACACTTTATG AGAAGAATTT GAATGGAATT 
CTTGCCGACG AGATGGGCCT GGGAAAGACG ATTCAAACGA TTTCCCTGCT GGCTCATATG 
GCTTGTAGTG AATCGATTTG GGGACCACAC TTGATTGTTG TGCCGACGTC TGTCATTCTG 
AATTGGGAGA TGGAGTTCAA GAAATGGTGT CCGGCTCTGA AGATTTTGAC GTATTTTGGT 
ACGGCGAAGG AGCGTGCCGA GAAGCGGAAG GGATGGATGA AGCCGAATTG TTTCCATGTG 
TGCATCACAT CATACAAGAC GGTTACTCAA GATATTAGAG CTTTTAAGCA GAGGGCCTGG 
CAGTACCTAA TTCTCGATGA AGCTCAAAAT ATCAAAAACT GGAAGTCCCA ACGTTGGCAG 
GCTCTTCTGA ATGTCCGTGC TCGACGTCGC CTTCTCCTGA CCGGAACTCC ACTTCAGAAC 
TCTCTAATGG AACTGTGGTC GTTGATGCAT TTTTTGATGC CAACAATATT CTCAAGTCAT 
GATGATTTCA AGGATTGGTT CTCGAATCCG TTGACAGGGA TGATGGAAGG AAATATGGAA 
TTCAATGCTC CACTAATCGG ACGACTTCAC AAAGTGCTCC GTCCGTTTAT TCTGCGGCGG 
CTCAAGAAGG AAGTTGAGAA GCAGCTGCCA GAGAAGACTG AGCATATTGT GAATTGTTCG 
TTGTCAAAGC GGCAGAGATA CCTGTACGAT GACTTTATGA GTCGTAGATC AACAAAGGAG 
AATCTAAAGT CTGGAAATAT GATGTCGGTG CTCAACATTG TGATGCAACT CCGAAAATGT 
TGTAATCATC CGAATCTCTT CGAGCCGCGG CCAGTTGTTG CTCCGTTCGT CGTTGAGAAG 
CTTCAGCTCG ATGTTCCGGC TCGTCTCTTT GAAATTTCGC AGCAAGATCC CTCCTCCTCC 
TCAGCTAGTC AAATTCCGGA AATTTTCAAT TTATCCAAAA TCGGCTATCA ATCTTCCGTT 
CGATCTGCAA AACCACTCAT CGAAGAGCTT GAAGCAATGA GCACTTATCC GGAGCCACGA 
GCACCAGAAG TTGGCGGATT TCGGTTCAAT CGGACGGCTT TTGTTGCAAA GAATCCGCAT 
ACGGAAGAGT CGGAGGACGA AGGTGTTATG AGAAGTCGTG TTCTGCCAAA ACCAATTAAT 
GGAACAGCTC AACCACTTCA AAATGGAAAT TCAATACCAC AAAATGCTCC AAATCGTCCA 
CAAACTTCAT GCATTCGTTC AAAAACCGTC GTAAATACAG TTCCACTGAC CATCTCCACC 
GATCGAAGTG GTTTTCATTT TAATATGGCC AATGTTGGAA GAGGTGTTGT TCGTTTGGAT 
GATTCAGCAC GTATGAGCCC ACCGCTCAAA CGTCAGAAGC TCACCGGAAC TGCAACGAAT 
TGGAGTGATT ATGTTCCGCG ACACGTTGTT GAAAAGATGG AAGAATCGAG AAAAAACCAG 
CTGGAAATTG TTCGAAGGCG ATTTGAGATG ATTCGTGCTC CGATTATTCC ACTGGAAATG 
GTTGCGCTGG TTCGAGAGGA AATTATTGCA GAATTTCCAC GTTTGGCTGT GGAAGAGGAC 
GAGGTTGTGC AGGAGAGGCT TTTGGAGTAT TGCGAGTTGT TGGTGCAAAG ATTCGGAATG 
TACGTCGAAC CAGTGCTGAC CGATGCTTGG CAGTGTCGTC CATCATCGTC TGGTCTTCCA 
TCATATATTC GCAACAATTT ATCAAATATC GAGCTGAATT CTCGTTCTCT TCTCCTCAAC 
ACCTCCACTA ATTTCGATAC CCGAATGTCG ATCTCACGTG CTCTTCAATT CCCAGAACTC 
CGTCTGATCG AGTACGATTG TGGAAAGCTT CAGACGTTGG CTGTTCTGCT TCGTCAGTTG 
TACCTGTACA AGCACAGATG TCTGATCTTC ACGCAAATGT CAAAGATGCT CGACGTTCTG 
CAGACCTTCC TTTCTCATCA CGGTTATCAG TATTTCCGCC TCGACGGTAC CACTGGTGTC 
GAACAAAGAC AGGCGATGAT GGAGCGGTTC AACGCGGATC CCAAGGTGTT TTGCTTCATT 
CTGTCGACGA GATCCGGTGG TGTTGGAGTC AATCTAACCG GTGCTGACAC TGTGATCTTC 
TACGATTCGG ATTGGAATCC GACGATGGAT GCTCAGGCTC AGGATAGATG TCATCGTATC 
GGACAGACGA GGAATGTCTC GATTTATCGA TTGATTTCCG AGCGAACAAT TGAGGAGAAT 
ATTCTGAGAA AGGCAACACA GAAGCGGCGA CTTGGAGAGT TGGCAATTGA CGAGGCTGGC 
TTCACACCCG AGTTCTTCAA ACAATCTGAC AGTATTCGGG ATCTTTTTGA TGGAGAGAAT 
GTGGAAGTGA CTGCTGTGGC AGATGTTGCG ACGACGATGA GCGAGAAAGA AATGGAGGTT 
GCGATGGCAA AGTGTGAAGA TGAAGCTGAT GTGAATGCGG CGAAGATTGC GGTGGCCGAG 
GCGAACGTTG ATAATGCGGA GTTTGATGAG AAATCATTGC CGCCGATGAG CAATTTGCAA 
GGAGATGAGG AGGCTGATGA GAAGTATATG GAGTTGATAC AACAGCTCAA ACCAATCGAA 
CGATATGCCA TTAACTTTCT TGAGACACAG TACAAGCCAG AATTTGAGGA AGAATGCAAA 
GAGGCAGAGG CTCTTATCGA CCAAAAACGC GAAGAATGGG ACAAAAATCT CAACGATACC 
GCCGTCATTG ACCTCGACGA TTCGGATAGT CTGCTGCTCA ACGATCCTTC GACTTCTGCC 
GATTTTTATC AGAGCTCAAG TCTTTTAGAC GAGATAAAAT TCTACGACGA GCTGGACGAT 
ATCATGCCAA TCTGGCTTCC ACCATCACCA CCAGATTCGG ATGCGGATTT CGACTTGAGA 
ATGGAAGATG ATTGTCTCGA TCTGATGTAT GAAATTGAAC AAATGAACGA GGCTCGCCTA 
CCACAAGTTT GTCATGAAAT GAGACGTCCG TTGGCTGAAA AACAGCAGAA ACAGAACACG 
TTGAATGCGT TTAATGACAT TCTATCGGCA AAAGAAAAGG AATCGGTGTA CGATGCGGTC 
AACAAGTGCC TTCAAATGCC ACAATCCGAA GCGATCACAG CAGAATCTGC AGCGTCTCCA 
GCATACACGG AACACTCATC ATTCTCGATG GATGATACAA GCCAGGATGC GAAGATTGAG 
CCAAGTTTGA CTGAAAATCA ACAACCCACC ACCACCGCCA CTACTACTAC TACAGTACCC 
CAACAACAAC AACAACAGCA GCAGCAAAAA TCGTCGAAAA AGAAGAGAAA TGATAATCGA 
ACGGCTCAAA ATCGAACAGC TGAAAATGGT GTGAAACGAG CGACAACTCC ACCACCATCA 
TGGCGTGAAG AGCCAGATTA TGATGGAGCC GAATGGAATA TAGTTGAAGA TTATGCACTA 
CTTCAAGCAG TTCAAGTCGA ATTTGCAAAT GCTCATTTAG TCGAAAAATC GGCGAATGAG 
GGAATGGTGT TGAACTGGGA ATTCGTGTCG AATGCCGTTA ATAAGCAGAC AAGATTTTTC 
CGCTCGGCCC GTCAATGCTC AATTCGATAT CAAATGTTTG TTCGGCCAAA AGAGCTCGGA 
CAGTTGGTGG CTTCTGATCC GATTTCCAAG AAAACGATGA AAGTCGACCT ATCGCATACT 
GAATTATCTC ATTTGAGAAA AGGACGAATG ACTACGGAGA GCCAATATGC TCATGATTAT 
GGAATATTGA CTGATAAGAA ACATGTGAAT AGATTTAAAA GTGTTCGAGT GGCGGCAACA 
CGGAGACCTG TTCAGTTTTG GAGAGGCCCT AAAGCTCTAG AATCTCGAAA TCTGCAATCT 
CTAAACGGCG GAATGCCACC ACGACACGAG TCGAGACTCG CCGAATTCGA CGTAAAAACC 
AATATTCGCC TGGACGCCGA GGACATTGTC ACAATGTCCG ACGAGTCGAT TGTCGCCTAT 
GAAGCGAGCA AGAAGAAGCT ACTGGCCAGT CGTCAAACAA AACCCTCACC ACGTCAAGAT 
GTCCGATTCC ATACGCTGGT TCTTCGGCCG TATACCGTAC CTGTGACAAC TGAGTACTCG 
GCTGCACCTT CTCGTCGTGA AATGCGCATC GCTGTTCCAC CGCTTCAGCC TTCGGCTTTA 
TCTACGATTT CCTCAGTTGC TGCTGCTGCC ACGTCTGGGC CACTACCATC AATTCAGCAT 
TTGCAGTCGT CGTCTACGGG CTTGGGATCT CAGCAAAATT TGCAAAATTC GCATAATTCT 
GAGCAAAGAA ATAATGTGCA AAATATGCAT CAAAATCAAT ATAATTCAAG TCAAAATCCG 
CCAATACCTA TCCGACAAAT CGGAGCAGCA TCATCACACC AACATGATCA AGGATCTCAG 
GGGCCTGGGG GAAAACCACA AGCCTATCAC CTGGTGCAAC AGGGATCACA GCAACAGCAG 
CAGCAGCAGC AGCAGGCGAC GTTACAGCGA AGAAATGCGG CGGCGGCGGC AGGGTCGAAT 
GTGCAGTTTA TTCAGCAGCA GCAGCAGCAG CAGCAATCGG GTAAAAATTG CGGTCAAGGA 
CAAAGCTTCG TTGTCATGGG CTCGCAGAGC TCATCAAATG ATGGACAAGG TGGAGCATCG 
ACCGTCGGAG GAGGAGGAGG AGGATCACAA CAGCCTCACC AGCAGCAGCA GCAGCAGCCA 
CAACAAAGAA TACAGTACAT TCCACAAGTT ACCGGTAGCG GAAATAACGG TGGAGGTGGT 
GGAAGAGGAG GCTACGGTAG TACACTGGTC ATGCCAAGAG GAGGACGTGT TGTCAGGCCA 
GCGGGAACTC TACCAGGCGG TGGACGTCTG TATGTCGATC ATAACCGTCA TCCATATCCA 
ATGTCGTCAA ATGTTGTGCC AGTACGTGTT CTACCAGCCA CGCAACAAGG ACAACAACGA 
ATGATGACAG GACAACGTCG TCCGGCTCCA GCGCCCGGTA CTGTCGCCGC AATGGTGTTG 
CCGAATCGAG GAGCTGGTGG AATTCCGCAA ATGCGCAGTT TGCAACGAGG CAGTTACACC 
GGCGGTGGTG GTCAGCAACG AATCAACGTG ATGGTTCAAC CACAACAAAT GCGCAGCAAC 
AATGGCGGTG GAGTCGGTGG CCAAGGAGGC CTCCAGGGTG GTCCAGGAGG TCCGCAAGGA 
ATTCGTCGGC CACTCGTCGG ACGGCCACTA CAACGAGGAG TCGATAATCA GGCGCCGACG 
GTTGCTCAGG TCGTTGTTGC TCCGCCGCAA GGAATGCAGC AGGCATCACA AGGACCACCC 
GTACTTCATA TGCAGAGAGC GGTTTCCATG CAAATGCCGA CGAGTCATCA TCATCAAGGC 
CAACAGCAGG CTCCTCCGCA GAGCTCACAG CAGGCTTCGC AACAGGCTCC CACATCGGAT 
TCTGGGACGA GTGCTCCGCC ACGACAAGCA CCACCACCAC AAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssl-1 ( NM_001268252.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255181.1 Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.

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CloneID	OCa340604
Clone ID Related Accession (Same CDS sequence)	NM_001268252.1 , NM_001268252.2
Accession Version	NM_001268252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Helicase ssl-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001268252.1. COMPLETENESS: incomplete on the 5' end.

ATGGCAGATC TCACAAAAGA ACCACCACCA ACACCTGCAC CTCCTCCTCC AATCCAGAAG 
ACAATGCAAC CGATTGAGGT GAAAATTGAG GATTCAGAGG GCTCAAATAC GGCTCAACCG 
AGTGTTCTGC CCAGTTGTGG AGGAGGAGAG ACGAATGTGG AAAGAGCCGC CAAAAGAGAA 
GCGCATGTAT TGGCTCGAAT CGCCGAGCTC CGTAAGAACG GCTTATGGTC GAACAGTCGT 
CTGCCAAAGT GCGTCGAACC TGAACGTAAT AAAACGCATT GGGATTATCT ACTGGAAGAG 
GTCAAATGGA TGGCAGTTGA TTTCCGAACC GAGACGAATA CGAAGCGAAA AATCGCCAAA 
GTTATAGCTC ACGCCATTGC GAAACAGCAC CGCGACAAGC AGATCGAGAT TGAGAGAGCC 
GCCGAACGGG AGATCAAGGA GAAGCGAAAA ATGTGTGCAG GAATCGCGAA GATGGTACGG 
GATTTCTGGT CGTCTACGGA TAAAGTTGTG GATATTCGAG CGAAGGAAGT TCTGGAGTCG 
AGGCTCAGGA AGGCGAGAAA TAAGCATTTG ATGTTTGTAA TTGGACAAGT CGATGAAATG 
AGCAATATTG TGCAAGAAGG ACTTGTTTCA TCGTCGAAAT CCCCATCAAT TGCATCGGAT 
CGAGATGATA AAGATGAAGA ATTCAAAGCA CCTGGCTCTG ATTCAGAATC TGACGATGAG 
CAGACAATTG CAAACGCGGA AAAGTCACAG AAAAAGGAAG ATGTTCGACA GGAAGTTGAT 
GCTCTTCAAA ACGAGGCAAC TGTGGATATG GATGACTTTT TGTACACTTT ACCGCCGGAA 
TATCTGAAGG CTTATGGTCT GACGCAGGAG GATTTGGAGG AGATGAAGCG CGAGAAATTG 
GAGGAGCAGA AGGCTCGGAA GGAAGCTTGT GGTGATAATG AGGAGAAAAT GGAGATTGAT 
GAAAGCCCAT CATCAGATGC TCAAAAGCCT TCCACCTCAA GCTCAGATCT CACCGCCGAG 
CAGCTTCAAG ATCCAACAGC TGAAGACGGC AACGGTGATG GTCATGGTGT ACTTGAAAAC 
GTGGATTACG TGAAGCTCAA CAGTCAGGAT AGTGATGAAC GACAACAAGA GTTGGCGAAT 
ATCGCAGAAG AAGCGCTGAA ATTCCAGCCA AAAGGATATA CACTTGAGAC GACACAAGTC 
AAGACGCCCG TACCATTCCT GATTCGAGGA CAACTGAGAG AATATCAAAT GGTTGGATTG 
GATTGGATGG TTACACTTTA TGAGAAGAAT TTGAATGGAA TTCTTGCCGA CGAGATGGGC 
CTGGGAAAGA CGATTCAAAC GATTTCCCTG CTGGCTCATA TGGCTTGTAG TGAATCGATT 
TGGGGACCAC ACTTGATTGT TGTGCCGACG TCTGTCATTC TGAATTGGGA GATGGAGTTC 
AAGAAATGGT GTCCGGCTCT GAAGATTTTG ACGTATTTTG GTACGGCGAA GGAGCGTGCC 
GAGAAGCGGA AGGGATGGAT GAAGCCGAAT TGTTTCCATG TGTGCATCAC ATCATACAAG 
ACGGTTACTC AAGATATTAG AGCTTTTAAG CAGAGGGCCT GGCAGTACCT AATTCTCGAT 
GAAGCTCAAA ATATCAAAAA CTGGAAGTCC CAACGTTGGC AGGCTCTTCT GAATGTCCGT 
GCTCGACGTC GCCTTCTCCT GACCGGAACT CCACTTCAGA ACTCTCTAAT GGAACTGTGG 
TCGTTGATGC ATTTTTTGAT GCCAACAATA TTCTCAAGTC ATGATGATTT CAAGGATTGG 
TTCTCGAATC CGTTGACAGG GATGATGGAA GGAAATATGG AATTCAATGC TCCACTAATC 
GGACGACTTC ACAAAGTGCT CCGTCCGTTT ATTCTGCGGC GGCTCAAGAA GGAAGTTGAG 
AAGCAGCTGC CAGAGAAGAC TGAGCATATT GTGAATTGTT CGTTGTCAAA GCGGCAGAGA 
TACCTGTACG ATGACTTTAT GAGTCGTAGA TCAACAAAGG AGAATCTAAA GTCTGGAAAT 
ATGATGTCGG TGCTCAACAT TGTGATGCAA CTCCGAAAAT GTTGTAATCA TCCGAATCTC 
TTCGAGCCGC GGCCAGTTGT TGCTCCGTTC GTCGTTGAGA AGCTTCAGCT CGATGTTCCG 
GCTCGTCTCT TTGAAATTTC GCAGCAAGAT CCCTCCTCCT CCTCAGCTAG TCAAATTCCG 
GAAATTTTCA ATTTATCCAA AATCGGCTAT CAATCTTCCG TTCGATCTGC AAAACCACTC 
ATCGAAGAGC TTGAAGCAAT GAGCACTTAT CCGGAGCCAC GAGCACCAGA AGTTGGCGGA 
TTTCGGTTCA ATCGGACGGC TTTTGTTGCA AAGAATCCGC ATACGGAAGA GTCGGAGGAC 
GAAGGTGTTA TGAGAAGTCG TGTTCTGCCA AAACCAATTA ATGGAACAGC TCAACCACTT 
CAAAATGGAA ATTCAATACC ACAAAATGCT CCAAATCGTC CACAAACTTC ATGCATTCGT 
TCAAAAACCG TCGTAAATAC AGTTCCACTG ACCATCTCCA CCGATCGAAG TGGTTTTCAT 
TTTAATATGG CCAATGTTGG AAGAGGTGTT GTTCGTTTGG ATGATTCAGC ACGTATGAGC 
CCACCGCTCA AACGTCAGAA GCTCACCGGA ACTGCAACGA ATTGGAGTGA TTATGTTCCG 
CGACACGTTG TTGAAAAGAT GGAAGAATCG AGAAAAAACC AGCTGGAAAT TGTTCGAAGG 
CGATTTGAGA TGATTCGTGC TCCGATTATT CCACTGGAAA TGGTTGCGCT GGTTCGAGAG 
GAAATTATTG CAGAATTTCC ACGTTTGGCT GTGGAAGAGG ACGAGGTTGT GCAGGAGAGG 
CTTTTGGAGT ATTGCGAGTT GTTGGTGCAA AGATTCGGAA TGTACGTCGA ACCAGTGCTG 
ACCGATGCTT GGCAGTGTCG TCCATCATCG TCTGGTCTTC CATCATATAT TCGCAACAAT 
TTATCAAATA TCGAGCTGAA TTCTCGTTCT CTTCTCCTCA ACACCTCCAC TAATTTCGAT 
ACCCGAATGT CGATCTCACG TGCTCTTCAA TTCCCAGAAC TCCGTCTGAT CGAGTACGAT 
TGTGGAAAGC TTCAGACGTT GGCTGTTCTG CTTCGTCAGT TGTACCTGTA CAAGCACAGA 
TGTCTGATCT TCACGCAAAT GTCAAAGATG CTCGACGTTC TGCAGACCTT CCTTTCTCAT 
CACGGTTATC AGTATTTCCG CCTCGACGGT ACCACTGGTG TCGAACAAAG ACAGGCGATG 
ATGGAGCGGT TCAACGCGGA TCCCAAGGTG TTTTGCTTCA TTCTGTCGAC GAGATCCGGT 
GGTGTTGGAG TCAATCTAAC CGGTGCTGAC ACTGTGATCT TCTACGATTC GGATTGGAAT 
CCGACGATGG ATGCTCAGGC TCAGGATAGA TGTCATCGTA TCGGACAGAC GAGGAATGTC 
TCGATTTATC GATTGATTTC CGAGCGAACA ATTGAGGAGA ATATTCTGAG AAAGGCAACA 
CAGAAGCGGC GACTTGGAGA GTTGGCAATT GACGAGGCTG GCTTCACACC CGAGTTCTTC 
AAACAATCTG ACAGTATTCG GGATCTTTTT GATGGAGAGA ATGTGGAAGT GACTGCTGTG 
GCAGATGTTG CGACGACGAT GAGCGAGAAA GAAATGGAGG TTGCGATGGC AAAGTGTGAA 
GATGAAGCTG ATGTGAATGC GGCGAAGATT GCGGTGGCCG AGGCGAACGT TGATAATGCG 
GAGTTTGATG AGAAATCATT GCCGCCGATG AGCAATTTGC AAGGAGATGA GGAGGCTGAT 
GAGAAGTATA TGGAGTTGAT ACAACAGCTC AAACCAATCG AACGATATGC CATTAACTTT 
CTTGAGACAC AGTACAAGCC AGAATTTGAG GAAGAATGCA AAGAGGCAGA GGCTCTTATC 
GACCAAAAAC GCGAAGAATG GGACAAAAAT CTCAACGATA CCGCCGTCAT TGACCTCGAC 
GATTCGGATA GTCTGCTGCT CAACGATCCT TCGACTTCTG CCGATTTTTA TCAGAGCTCA 
AGTCTTTTAG ACGAGATAAA ATTCTACGAC GAGCTGGACG ATATCATGCC AATCTGGCTT 
CCACCATCAC CACCAGATTC GGATGCGGAT TTCGACTTGA GAATGGAAGA TGATTGTCTC 
GATCTGATGT ATGAAATTGA ACAAATGAAC GAGGCTCGCC TACCACAAGT TTGTCATGAA 
ATGAGACGTC CGTTGGCTGA AAAACAGCAG AAACAGAACA CGTTGAATGC GTTTAATGAC 
ATTCTATCGG CAAAAGAAAA GGAATCGGTG TACGATGCGG TCAACAAGTG CCTTCAAATG 
CCACAATCCG AAGCGATCAC AGCAGAATCT GCAGCGTCTC CAGCATACAC GGAACACTCA 
TCATTCTCGA TGGATGATAC AAGCCAGGAT GCGAAGATTG AGCCAAGTTT GACTGAAAAT 
CAACAACCCA CCACCACCGC CACTACTACT ACTACAGTAC CCCAACAACA ACAACAACAG 
CAGCAGCAAA AATCGTCGAA AAAGAAGAGA AATGATAATC GAACGGCTCA AAATCGAACA 
GCTGAAAATG GTGTGAAACG AGCGACAACT CCACCACCAT CATGGCGTGA AGAGCCAGAT 
TATGATGGAG CCGAATGGAA TATAGTTGAA GATTATGCAC TACTTCAAGC AGTTCAAGTC 
GAATTTGCAA ATGCTCATTT AGTCGAAAAA TCGGCGAATG AGGGAATGGT GTTGAACTGG 
GAATTCGTGT CGAATGCCGT TAATAAGCAG ACAAGATTTT TCCGCTCGGC CCGTCAATGC 
TCAATTCGAT ATCAAATGTT TGTTCGGCCA AAAGAGCTCG GACAGTTGGT GGCTTCTGAT 
CCGATTTCCA AGAAAACGAT GAAAGTCGAC CTATCGCATA CTGAATTATC TCATTTGAGA 
AAAGGACGAA TGACTACGGA GAGCCAATAT GCTCATGATT ATGGAATATT GACTGATAAG 
AAACATGTGA ATAGATTTAA AAGTGTTCGA GTGGCGGCAA CACGGAGACC TGTTCAGTTT 
TGGAGAGGCC CTAAAGCTCT AGAATCTCGA AATCTGCAAT CTCTAAACGG CGGAATGCCA 
CCACGACACG AGTCGAGACT CGCCGAATTC GACGTAAAAA CCAATATTCG CCTGGACGCC 
GAGGACATTG TCACAATGTC CGACGAGTCG ATTGTCGCCT ATGAAGCGAG CAAGAAGAAG 
CTACTGGCCA GTCGTCAAAC AAAACCCTCA CCACGTCAAG ATGTCCGATT CCATACGCTG 
GTTCTTCGGC CGTATACCGT ACCTGTGACA ACTGAGTACT CGGCTGCACC TTCTCGTCGT 
GAAATGCGCA TCGCTGTTCC ACCGCTTCAG CCTTCGGCTT TATCTACGAT TTCCTCAGTT 
GCTGCTGCTG CCACGTCTGG GCCACTACCA TCAATTCAGC ATTTGCAGTC GTCGTCTACG 
GGCTTGGGAT CTCAGCAAAA TTTGCAAAAT TCGCATAATT CTGAGCAAAG AAATAATGTG 
CAAAATATGC ATCAAAATCA ATATAATTCA AGTCAAAATC CGCCAATACC TATCCGACAA 
ATCGGAGCAG CATCATCACA CCAACATGAT CAAGGATCTC AGGGGCCTGG GGGAAAACCA 
CAAGCCTATC ACCTGGTGCA ACAGGGATCA CAGCAACAGC AGCAGCAGCA GCAGCAGGCG 
ACGTTACAGC GAAGAAATGC GGCGGCGGCG GCAGGGTCGA ATGTGCAGTT TATTCAGCAG 
CAGCAGCAGC AGCAGCAATC GGGTAAAAAT TGCGGTCAAG GACAAAGCTT CGTTGTCATG 
GGCTCGCAGA GCTCATCAAA TGATGGACAA GGTGGAGCAT CGACCGTCGG AGGAGGAGGA 
GGAGGATCAC AACAGCCTCA CCAGCAGCAG CAGCAGCAGC CACAACAAAG AATACAGTAC 
ATTCCACAAG TTACCGGTAG CGGAAATAAC GGTGGAGGTG GTGGAAGAGG AGGCTACGGT 
AGTACACTGG TCATGCCAAG AGGAGGACGT GTTGTCAGGC CAGCGGGAAC TCTACCAGGC 
GGTGGACGTC TGTATGTCGA TCATAACCGT CATCCATATC CAATGTCGTC AAATGTTGTG 
CCAGTACGTG TTCTACCAGC CACGCAACAA GGACAACAAC GAATGATGAC AGGACAACGT 
CGTCCGGCTC CAGCGCCCGG TACTGTCGCC GCAATGGTGT TGCCGAATCG AGGAGCTGGT 
GGAATTCCGC AAATGCGCAG TTTGCAACGA GGCAGTTACA CCGGCGGTGG TGGTCAGCAA 
CGAATCAACG TGATGGTTCA ACCACAACAA ATGCGCAGCA ACAATGGCGG TGGAGTCGGT 
GGCCAAGGAG GCCTCCAGGG TGGTCCAGGA GGTCCGCAAG GAATTCGTCG GCCACTCGTC 
GGACGGCCAC TACAACGAGG AGTCGATAAT CAGGCGCCGA CGGTTGCTCA GGTCGTTGTT 
GCTCCGCCGC AAGGAATGCA GCAGGCATCA CAAGGACCAC CCGTACTTCA TATGCAGAGA 
GCGGTTTCCA TGCAAATGCC GACGAGTCAT CATCATCAAG GCCAACAGCA GGCTCCTCCG 
CAGAGCTCAC AGCAGGCTTC GCAACAGGCT CCCACATCGG ATTCTGGGAC GAGTGCTCCG 
CCACGACAAG CACCACCACC ACAAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255181.1
CDS	1..6750
Translation	MADLTKEPPPTPAPPPPIQKTMQPIEVKIEDSEGSNTAQPSVLPSCGGGETNVERAAKREAHVLARIAELRKNGLWSNSRLPKCVEPERNKTHWDYLLEEVKWMAVDFRTETNTKRKIAKVIAHAIAKQHRDKQIEIERAAEREIKEKRKMCAGIAKMVRDFWSSTDKVVDIRAKEVLESRLRKARNKHLMFVIGQVDEMSNIVQEGLVSSSKSPSIASDRDDKDEEFKAPGSDSESDDEQTIANAEKSQKKEDVRQEVDALQNEATVDMDDFLYTLPPEYLKAYGLTQEDLEEMKREKLEEQKARKEACGDNEEKMEIDESPSSDAQKPSTSSSDLTAEQLQDPTAEDGNGDGHGVLENVDYVKLNSQDSDERQQELANIAEEALKFQPKGYTLETTQVKTPVPFLIRGQLREYQMVGLDWMVTLYEKNLNGILADEMGLGKTIQTISLLAHMACSESIWGPHLIVVPTSVILNWEMEFKKWCPALKILTYFGTAKERAEKRKGWMKPNCFHVCITSYKTVTQDIRAFKQRAWQYLILDEAQNIKNWKSQRWQALLNVRARRRLLLTGTPLQNSLMELWSLMHFLMPTIFSSHDDFKDWFSNPLTGMMEGNMEFNAPLIGRLHKVLRPFILRRLKKEVEKQLPEKTEHIVNCSLSKRQRYLYDDFMSRRSTKENLKSGNMMSVLNIVMQLRKCCNHPNLFEPRPVVAPFVVEKLQLDVPARLFEISQQDPSSSSASQIPEIFNLSKIGYQSSVRSAKPLIEELEAMSTYPEPRAPEVGGFRFNRTAFVAKNPHTEESEDEGVMRSRVLPKPINGTAQPLQNGNSIPQNAPNRPQTSCIRSKTVVNTVPLTISTDRSGFHFNMANVGRGVVRLDDSARMSPPLKRQKLTGTATNWSDYVPRHVVEKMEESRKNQLEIVRRRFEMIRAPIIPLEMVALVREEIIAEFPRLAVEEDEVVQERLLEYCELLVQRFGMYVEPVLTDAWQCRPSSSGLPSYIRNNLSNIELNSRSLLLNTSTNFDTRMSISRALQFPELRLIEYDCGKLQTLAVLLRQLYLYKHRCLIFTQMSKMLDVLQTFLSHHGYQYFRLDGTTGVEQRQAMMERFNADPKVFCFILSTRSGGVGVNLTGADTVIFYDSDWNPTMDAQAQDRCHRIGQTRNVSIYRLISERTIEENILRKATQKRRLGELAIDEAGFTPEFFKQSDSIRDLFDGENVEVTAVADVATTMSEKEMEVAMAKCEDEADVNAAKIAVAEANVDNAEFDEKSLPPMSNLQGDEEADEKYMELIQQLKPIERYAINFLETQYKPEFEEECKEAEALIDQKREEWDKNLNDTAVIDLDDSDSLLLNDPSTSADFYQSSSLLDEIKFYDELDDIMPIWLPPSPPDSDADFDLRMEDDCLDLMYEIEQMNEARLPQVCHEMRRPLAEKQQKQNTLNAFNDILSAKEKESVYDAVNKCLQMPQSEAITAESAASPAYTEHSSFSMDDTSQDAKIEPSLTENQQPTTTATTTTTVPQQQQQQQQQKSSKKKRNDNRTAQNRTAENGVKRATTPPPSWREEPDYDGAEWNIVEDYALLQAVQVEFANAHLVEKSANEGMVLNWEFVSNAVNKQTRFFRSARQCSIRYQMFVRPKELGQLVASDPISKKTMKVDLSHTELSHLRKGRMTTESQYAHDYGILTDKKHVNRFKSVRVAATRRPVQFWRGPKALESRNLQSLNGGMPPRHESRLAEFDVKTNIRLDAEDIVTMSDESIVAYEASKKKLLASRQTKPSPRQDVRFHTLVLRPYTVPVTTEYSAAPSRREMRIAVPPLQPSALSTISSVAAAATSGPLPSIQHLQSSSTGLGSQQNLQNSHNSEQRNNVQNMHQNQYNSSQNPPIPIRQIGAASSHQHDQGSQGPGGKPQAYHLVQQGSQQQQQQQQQATLQRRNAAAAAGSNVQFIQQQQQQQQSGKNCGQGQSFVVMGSQSSSNDGQGGASTVGGGGGGSQQPHQQQQQQPQQRIQYIPQVTGSGNNGGGGGRGGYGSTLVMPRGGRVVRPAGTLPGGGRLYVDHNRHPYPMSSNVVPVRVLPATQQGQQRMMTGQRRPAPAPGTVAAMVLPNRGAGGIPQMRSLQRGSYTGGGGQQRINVMVQPQQMRSNNGGGVGGQGGLQGGPGGPQGIRRPLVGRPLQRGVDNQAPTVAQVVVAPPQGMQQASQGPPVLHMQRAVSMQMPTSHHHQGQQQAPPQSSQQASQQAPTSDSGTSAPPRQAPPPQN

Target ORF information:

RefSeq Version	NM_001268252.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268252.2

ORF Insert Sequence:

ATGGCAGATC TCACAAAAGA ACCACCACCA ACACCTGCAC CTCCTCCTCC AATCCAGAAG 
ACAATGCAAC CGATTGAGGT GAAAATTGAG GATTCAGAGG GCTCAAATAC GGCTCAACCG 
AGTGTTCTGC CCAGTTGTGG AGGAGGAGAG ACGAATGTGG AAAGAGCCGC CAAAAGAGAA 
GCGCATGTAT TGGCTCGAAT CGCCGAGCTC CGTAAGAACG GCTTATGGTC GAACAGTCGT 
CTGCCAAAGT GCGTCGAACC TGAACGTAAT AAAACGCATT GGGATTATCT ACTGGAAGAG 
GTCAAATGGA TGGCAGTTGA TTTCCGAACC GAGACGAATA CGAAGCGAAA AATCGCCAAA 
GTTATAGCTC ACGCCATTGC GAAACAGCAC CGCGACAAGC AGATCGAGAT TGAGAGAGCC 
GCCGAACGGG AGATCAAGGA GAAGCGAAAA ATGTGTGCAG GAATCGCGAA GATGGTACGG 
GATTTCTGGT CGTCTACGGA TAAAGTTGTG GATATTCGAG CGAAGGAAGT TCTGGAGTCG 
AGGCTCAGGA AGGCGAGAAA TAAGCATTTG ATGTTTGTAA TTGGACAAGT CGATGAAATG 
AGCAATATTG TGCAAGAAGG ACTTGTTTCA TCGTCGAAAT CCCCATCAAT TGCATCGGAT 
CGAGATGATA AAGATGAAGA ATTCAAAGCA CCTGGCTCTG ATTCAGAATC TGACGATGAG 
CAGACAATTG CAAACGCGGA AAAGTCACAG AAAAAGGAAG ATGTTCGACA GGAAGTTGAT 
GCTCTTCAAA ACGAGGCAAC TGTGGATATG GATGACTTTT TGTACACTTT ACCGCCGGAA 
TATCTGAAGG CTTATGGTCT GACGCAGGAG GATTTGGAGG AGATGAAGCG CGAGAAATTG 
GAGGAGCAGA AGGCTCGGAA GGAAGCTTGT GGTGATAATG AGGAGAAAAT GGAGATTGAT 
GAAAGCCCAT CATCAGATGC TCAAAAGCCT TCCACCTCAA GCTCAGATCT CACCGCCGAG 
CAGCTTCAAG ATCCAACAGC TGAAGACGGC AACGGTGATG GTCATGGTGT ACTTGAAAAC 
GTGGATTACG TGAAGCTCAA CAGTCAGGAT AGTGATGAAC GACAACAAGA GTTGGCGAAT 
ATCGCAGAAG AAGCGCTGAA ATTCCAGCCA AAAGGATATA CACTTGAGAC GACACAAGTC 
AAGACGCCCG TACCATTCCT GATTCGAGGA CAACTGAGAG AATATCAAAT GGTTGGATTG 
GATTGGATGG TTACACTTTA TGAGAAGAAT TTGAATGGAA TTCTTGCCGA CGAGATGGGC 
CTGGGAAAGA CGATTCAAAC GATTTCCCTG CTGGCTCATA TGGCTTGTAG TGAATCGATT 
TGGGGACCAC ACTTGATTGT TGTGCCGACG TCTGTCATTC TGAATTGGGA GATGGAGTTC 
AAGAAATGGT GTCCGGCTCT GAAGATTTTG ACGTATTTTG GTACGGCGAA GGAGCGTGCC 
GAGAAGCGGA AGGGATGGAT GAAGCCGAAT TGTTTCCATG TGTGCATCAC ATCATACAAG 
ACGGTTACTC AAGATATTAG AGCTTTTAAG CAGAGGGCCT GGCAGTACCT AATTCTCGAT 
GAAGCTCAAA ATATCAAAAA CTGGAAGTCC CAACGTTGGC AGGCTCTTCT GAATGTCCGT 
GCTCGACGTC GCCTTCTCCT GACCGGAACT CCACTTCAGA ACTCTCTAAT GGAACTGTGG 
TCGTTGATGC ATTTTTTGAT GCCAACAATA TTCTCAAGTC ATGATGATTT CAAGGATTGG 
TTCTCGAATC CGTTGACAGG GATGATGGAA GGAAATATGG AATTCAATGC TCCACTAATC 
GGACGACTTC ACAAAGTGCT CCGTCCGTTT ATTCTGCGGC GGCTCAAGAA GGAAGTTGAG 
AAGCAGCTGC CAGAGAAGAC TGAGCATATT GTGAATTGTT CGTTGTCAAA GCGGCAGAGA 
TACCTGTACG ATGACTTTAT GAGTCGTAGA TCAACAAAGG AGAATCTAAA GTCTGGAAAT 
ATGATGTCGG TGCTCAACAT TGTGATGCAA CTCCGAAAAT GTTGTAATCA TCCGAATCTC 
TTCGAGCCGC GGCCAGTTGT TGCTCCGTTC GTCGTTGAGA AGCTTCAGCT CGATGTTCCG 
GCTCGTCTCT TTGAAATTTC GCAGCAAGAT CCCTCCTCCT CCTCAGCTAG TCAAATTCCG 
GAAATTTTCA ATTTATCCAA AATCGGCTAT CAATCTTCCG TTCGATCTGC AAAACCACTC 
ATCGAAGAGC TTGAAGCAAT GAGCACTTAT CCGGAGCCAC GAGCACCAGA AGTTGGCGGA 
TTTCGGTTCA ATCGGACGGC TTTTGTTGCA AAGAATCCGC ATACGGAAGA GTCGGAGGAC 
GAAGGTGTTA TGAGAAGTCG TGTTCTGCCA AAACCAATTA ATGGAACAGC TCAACCACTT 
CAAAATGGAA ATTCAATACC ACAAAATGCT CCAAATCGTC CACAAACTTC ATGCATTCGT 
TCAAAAACCG TCGTAAATAC AGTTCCACTG ACCATCTCCA CCGATCGAAG TGGTTTTCAT 
TTTAATATGG CCAATGTTGG AAGAGGTGTT GTTCGTTTGG ATGATTCAGC ACGTATGAGC 
CCACCGCTCA AACGTCAGAA GCTCACCGGA ACTGCAACGA ATTGGAGTGA TTATGTTCCG 
CGACACGTTG TTGAAAAGAT GGAAGAATCG AGAAAAAACC AGCTGGAAAT TGTTCGAAGG 
CGATTTGAGA TGATTCGTGC TCCGATTATT CCACTGGAAA TGGTTGCGCT GGTTCGAGAG 
GAAATTATTG CAGAATTTCC ACGTTTGGCT GTGGAAGAGG ACGAGGTTGT GCAGGAGAGG 
CTTTTGGAGT ATTGCGAGTT GTTGGTGCAA AGATTCGGAA TGTACGTCGA ACCAGTGCTG 
ACCGATGCTT GGCAGTGTCG TCCATCATCG TCTGGTCTTC CATCATATAT TCGCAACAAT 
TTATCAAATA TCGAGCTGAA TTCTCGTTCT CTTCTCCTCA ACACCTCCAC TAATTTCGAT 
ACCCGAATGT CGATCTCACG TGCTCTTCAA TTCCCAGAAC TCCGTCTGAT CGAGTACGAT 
TGTGGAAAGC TTCAGACGTT GGCTGTTCTG CTTCGTCAGT TGTACCTGTA CAAGCACAGA 
TGTCTGATCT TCACGCAAAT GTCAAAGATG CTCGACGTTC TGCAGACCTT CCTTTCTCAT 
CACGGTTATC AGTATTTCCG CCTCGACGGT ACCACTGGTG TCGAACAAAG ACAGGCGATG 
ATGGAGCGGT TCAACGCGGA TCCCAAGGTG TTTTGCTTCA TTCTGTCGAC GAGATCCGGT 
GGTGTTGGAG TCAATCTAAC CGGTGCTGAC ACTGTGATCT TCTACGATTC GGATTGGAAT 
CCGACGATGG ATGCTCAGGC TCAGGATAGA TGTCATCGTA TCGGACAGAC GAGGAATGTC 
TCGATTTATC GATTGATTTC CGAGCGAACA ATTGAGGAGA ATATTCTGAG AAAGGCAACA 
CAGAAGCGGC GACTTGGAGA GTTGGCAATT GACGAGGCTG GCTTCACACC CGAGTTCTTC 
AAACAATCTG ACAGTATTCG GGATCTTTTT GATGGAGAGA ATGTGGAAGT GACTGCTGTG 
GCAGATGTTG CGACGACGAT GAGCGAGAAA GAAATGGAGG TTGCGATGGC AAAGTGTGAA 
GATGAAGCTG ATGTGAATGC GGCGAAGATT GCGGTGGCCG AGGCGAACGT TGATAATGCG 
GAGTTTGATG AGAAATCATT GCCGCCGATG AGCAATTTGC AAGGAGATGA GGAGGCTGAT 
GAGAAGTATA TGGAGTTGAT ACAACAGCTC AAACCAATCG AACGATATGC CATTAACTTT 
CTTGAGACAC AGTACAAGCC AGAATTTGAG GAAGAATGCA AAGAGGCAGA GGCTCTTATC 
GACCAAAAAC GCGAAGAATG GGACAAAAAT CTCAACGATA CCGCCGTCAT TGACCTCGAC 
GATTCGGATA GTCTGCTGCT CAACGATCCT TCGACTTCTG CCGATTTTTA TCAGAGCTCA 
AGTCTTTTAG ACGAGATAAA ATTCTACGAC GAGCTGGACG ATATCATGCC AATCTGGCTT 
CCACCATCAC CACCAGATTC GGATGCGGAT TTCGACTTGA GAATGGAAGA TGATTGTCTC 
GATCTGATGT ATGAAATTGA ACAAATGAAC GAGGCTCGCC TACCACAAGT TTGTCATGAA 
ATGAGACGTC CGTTGGCTGA AAAACAGCAG AAACAGAACA CGTTGAATGC GTTTAATGAC 
ATTCTATCGG CAAAAGAAAA GGAATCGGTG TACGATGCGG TCAACAAGTG CCTTCAAATG 
CCACAATCCG AAGCGATCAC AGCAGAATCT GCAGCGTCTC CAGCATACAC GGAACACTCA 
TCATTCTCGA TGGATGATAC AAGCCAGGAT GCGAAGATTG AGCCAAGTTT GACTGAAAAT 
CAACAACCCA CCACCACCGC CACTACTACT ACTACAGTAC CCCAACAACA ACAACAACAG 
CAGCAGCAAA AATCGTCGAA AAAGAAGAGA AATGATAATC GAACGGCTCA AAATCGAACA 
GCTGAAAATG GTGTGAAACG AGCGACAACT CCACCACCAT CATGGCGTGA AGAGCCAGAT 
TATGATGGAG CCGAATGGAA TATAGTTGAA GATTATGCAC TACTTCAAGC AGTTCAAGTC 
GAATTTGCAA ATGCTCATTT AGTCGAAAAA TCGGCGAATG AGGGAATGGT GTTGAACTGG 
GAATTCGTGT CGAATGCCGT TAATAAGCAG ACAAGATTTT TCCGCTCGGC CCGTCAATGC 
TCAATTCGAT ATCAAATGTT TGTTCGGCCA AAAGAGCTCG GACAGTTGGT GGCTTCTGAT 
CCGATTTCCA AGAAAACGAT GAAAGTCGAC CTATCGCATA CTGAATTATC TCATTTGAGA 
AAAGGACGAA TGACTACGGA GAGCCAATAT GCTCATGATT ATGGAATATT GACTGATAAG 
AAACATGTGA ATAGATTTAA AAGTGTTCGA GTGGCGGCAA CACGGAGACC TGTTCAGTTT 
TGGAGAGGCC CTAAAGCTCT AGAATCTCGA AATCTGCAAT CTCTAAACGG CGGAATGCCA 
CCACGACACG AGTCGAGACT CGCCGAATTC GACGTAAAAA CCAATATTCG CCTGGACGCC 
GAGGACATTG TCACAATGTC CGACGAGTCG ATTGTCGCCT ATGAAGCGAG CAAGAAGAAG 
CTACTGGCCA GTCGTCAAAC AAAACCCTCA CCACGTCAAG ATGTCCGATT CCATACGCTG 
GTTCTTCGGC CGTATACCGT ACCTGTGACA ACTGAGTACT CGGCTGCACC TTCTCGTCGT 
GAAATGCGCA TCGCTGTTCC ACCGCTTCAG CCTTCGGCTT TATCTACGAT TTCCTCAGTT 
GCTGCTGCTG CCACGTCTGG GCCACTACCA TCAATTCAGC ATTTGCAGTC GTCGTCTACG 
GGCTTGGGAT CTCAGCAAAA TTTGCAAAAT TCGCATAATT CTGAGCAAAG AAATAATGTG 
CAAAATATGC ATCAAAATCA ATATAATTCA AGTCAAAATC CGCCAATACC TATCCGACAA 
ATCGGAGCAG CATCATCACA CCAACATGAT CAAGGATCTC AGGGGCCTGG GGGAAAACCA 
CAAGCCTATC ACCTGGTGCA ACAGGGATCA CAGCAACAGC AGCAGCAGCA GCAGCAGGCG 
ACGTTACAGC GAAGAAATGC GGCGGCGGCG GCAGGGTCGA ATGTGCAGTT TATTCAGCAG 
CAGCAGCAGC AGCAGCAATC GGGTAAAAAT TGCGGTCAAG GACAAAGCTT CGTTGTCATG 
GGCTCGCAGA GCTCATCAAA TGATGGACAA GGTGGAGCAT CGACCGTCGG AGGAGGAGGA 
GGAGGATCAC AACAGCCTCA CCAGCAGCAG CAGCAGCAGC CACAACAAAG AATACAGTAC 
ATTCCACAAG TTACCGGTAG CGGAAATAAC GGTGGAGGTG GTGGAAGAGG AGGCTACGGT 
AGTACACTGG TCATGCCAAG AGGAGGACGT GTTGTCAGGC CAGCGGGAAC TCTACCAGGC 
GGTGGACGTC TGTATGTCGA TCATAACCGT CATCCATATC CAATGTCGTC AAATGTTGTG 
CCAGTACGTG TTCTACCAGC CACGCAACAA GGACAACAAC GAATGATGAC AGGACAACGT 
CGTCCGGCTC CAGCGCCCGG TACTGTCGCC GCAATGGTGT TGCCGAATCG AGGAGCTGGT 
GGAATTCCGC AAATGCGCAG TTTGCAACGA GGCAGTTACA CCGGCGGTGG TGGTCAGCAA 
CGAATCAACG TGATGGTTCA ACCACAACAA ATGCGCAGCA ACAATGGCGG TGGAGTCGGT 
GGCCAAGGAG GCCTCCAGGG TGGTCCAGGA GGTCCGCAAG GAATTCGTCG GCCACTCGTC 
GGACGGCCAC TACAACGAGG AGTCGATAAT CAGGCGCCGA CGGTTGCTCA GGTCGTTGTT 
GCTCCGCCGC AAGGAATGCA GCAGGCATCA CAAGGACCAC CCGTACTTCA TATGCAGAGA 
GCGGTTTCCA TGCAAATGCC GACGAGTCAT CATCATCAAG GCCAACAGCA GGCTCCTCCG 
CAGAGCTCAC AGCAGGCTTC GCAACAGGCT CCCACATCGG ATTCTGGGAC GAGTGCTCCG 
CCACGACAAG CACCACCACC ACAAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssl-1 ( NM_001268250.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255179.1 Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.

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CloneID	OCa340236
Clone ID Related Accession (Same CDS sequence)	NM_001268250.1 , NM_001268250.2
Accession Version	NM_001268250.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Helicase ssl-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). COMPLETENESS: incomplete on both ends.

ATGCCGGCAA CACCGGTGCG TGCTTCAAGT ACTCGAATAA GCAGACGTAC ATCATCAAGA 
TCAGTGGCTG ATGATCAGCC ATCAACTTCG TCTGCGGTGG CTCCACCTCC TTCACCCATT 
GCCATAGAAA CTGATGAAGA TGCGGTAGTT GAGGAGGAGA AAAAGAAGAA AAAGACATCA 
GATGATTTGG AAATTATCAC TCCAAGAACT CCAGTCGATC GGCGAATTCC CTACATTTGC 
TCGATTCTTT TGACTGAAAA TCGATCGATT CGCGATAAAT TGGTTCTGAG CAGCGGTCCA 
GTTCGTCAAG AAGATCACGA AGAACAGATT GCTCGAGCTC AACGGATACA GCCAGTTGTC 
GATCAAATTC AACGAGTCGA GCAAATCATA CTCAATGGTT CAGTGGAAGA TATTCTGAAA 
GATCCTCGAT TCGCAGTAAT GGCAGATCTC ACAAAAGAAC CACCACCAAC ACCTGCACCT 
CCTCCTCCAA TCCAGAAGAC AATGCAACCG ATTGAGGTGA AAATTGAGGA TTCAGAGGGC 
TCAAATACGG CTCAACCGAG TGTTCTGCCC AGTTGTGGAG GAGGAGAGAC GAATGTGGAA 
AGAGCCGCCA AAAGAGAAGC GCATGTATTG GCTCGAATCG CCGAGCTCCG TAAGAACGGC 
TTATGGTCGA ACAGTCGTCT GCCAAAGTGC GTCGAACCTG AACGTAATAA AACGCATTGG 
GATTATCTAC TGGAAGAGGT CAAATGGATG GCAGTTGATT TCCGAACCGA GACGAATACG 
AAGCGAAAAA TCGCCAAAGT TATAGCTCAC GCCATTGCGA AACAGCACCG CGACAAGCAG 
ATCGAGATTG AGAGAGCCGC CGAACGGGAG ATCAAGGAGA AGCGAAAAAT GTGTGCAGGA 
ATCGCGAAGA TGGTACGGGA TTTCTGGTCG TCTACGGATA AAGTTGTGGA TATTCGAGCG 
AAGGAAGTTC TGGAGTCGAG GCTCAGGAAG GCGAGAAATA AGCATTTGAT GTTTGTAATT 
GGACAAGTCG ATGAAATGAG CAATATTGTG CAAGAAGGAC TTGTTTCATC GTCGAAATCC 
CCATCAATTG CATCGGATCG AGATGATAAA GATGAAGAAT TCAAAGCACC TGGCTCTGAT 
TCAGAATCTG ACGATGAGCA GACAATTGCA AACGCGGAAA AGTCACAGAA AAAGGAAGAT 
GTTCGACAGG AAGTTGATGC TCTTCAAAAC GAGGCAACTG TGGATATGGA TGACTTTTTG 
TACACTTTAC CGCCGGAATA TCTGAAGGCT TATGGTCTGA CGCAGGAGGA TTTGGAGGAG 
ATGAAGCGCG AGAAATTGGA GGAGCAGAAG GCTCGGAAGG AAGCTTGTGG TGATAATGAG 
GAGAAAATGG AGATTGATGA AAGCCCATCA TCAGATGCTC AAAAGCCTTC CACCTCAAGC 
TCAGATCTCA CCGCCGAGCA GCTTCAAGAT CCAACAGCTG AAGACGGCAA CGGTGATGGT 
CATGGTGTAC TTGAAAACGT GGATTACGTG AAGCTCAACA GTCAGGATAG TGATGAACGA 
CAACAAGAGT TGGCGAATAT CGCAGAAGAA GCGCTGAAAT TCCAGCCAAA AGGATATACA 
CTTGAGACGA CACAAGTCAA GACGCCCGTA CCATTCCTGA TTCGAGGACA ACTGAGAGAA 
TATCAAATGG TTGGATTGGA TTGGATGGTT ACACTTTATG AGAAGAATTT GAATGGAATT 
CTTGCCGACG AGATGGGCCT GGGAAAGACG ATTCAAACGA TTTCCCTGCT GGCTCATATG 
GCTTGTAGTG AATCGATTTG GGGACCACAC TTGATTGTTG TGCCGACGTC TGTCATTCTG 
AATTGGGAGA TGGAGTTCAA GAAATGGTGT CCGGCTCTGA AGATTTTGAC GTATTTTGGT 
ACGGCGAAGG AGCGTGCCGA GAAGCGGAAG GGATGGATGA AGCCGAATTG TTTCCATGTG 
TGCATCACAT CATACAAGAC GGTTACTCAA GATATTAGAG CTTTTAAGCA GAGGGCCTGG 
CAGTACCTAA TTCTCGATGA AGCTCAAAAT ATCAAAAACT GGAAGTCCCA ACGTTGGCAG 
GCTCTTCTGA ATGTCCGTGC TCGACGTCGC CTTCTCCTGA CCGGAACTCC ACTTCAGAAC 
TCTCTAATGG AACTGTGGTC GTTGATGCAT TTTTTGATGC CAACAATATT CTCAAGTCAT 
GATGATTTCA AGGATTGGTT CTCGAATCCG TTGACAGGGA TGATGGAAGG AAATATGGAA 
TTCAATGCTC CACTAATCGG ACGACTTCAC AAAGTGCTCC GTCCGTTTAT TCTGCGGCGG 
CTCAAGAAGG AAGTTGAGAA GCAGCTGCCA GAGAAGACTG AGCATATTGT GAATTGTTCG 
TTGTCAAAGC GGCAGAGATA CCTGTACGAT GACTTTATGA GTCGTAGATC AACAAAGGAG 
AATCTAAAGT CTGGAAATAT GATGTCGGTG CTCAACATTG TGATGCAACT CCGAAAATGT 
TGTAATCATC CGAATCTCTT CGAGCCGCGG CCAGTTGTTG CTCCGTTCGT CGTTGAGAAG 
CTTCAGCTCG ATGTTCCGGC TCGTCTCTTT GAAATTTCGC AGCAAGATCC CTCCTCCTCC 
TCAGCTAGTC AAATTCCGGA AATTTTCAAT TTATCCAAAA TCGGCTATCA ATCTTCCGTT 
CGATCTGCAA AACCACTCAT CGAAGAGCTT GAAGCAATGA GCACTTATCC GGAGCCACGA 
GCACCAGAAG TTGGCGGATT TCGGTTCAAT CGGACGGCTT TTGTTGCAAA GAATCCGCAT 
ACGGAAGAGT CGGAGGACGA AGGTGTTATG AGAAGTCGTG TTCTGCCAAA ACCAATTAAT 
GGAACAGCTC AACCACTTCA AAATGGAAAT TCAATACCAC AAAATGCTCC AAATCGTCCA 
CAAACTTCAT GCATTCGTTC AAAAACCGTC GTAAATACAG TTCCACTGAC CATCTCCACC 
GATCGAAGTG GTTTTCATTT TAATATGGCC AATGTTGGAA GAGGTGTTGT TCGTTTGGAT 
GATTCAGCAC GTATGAGCCC ACCGCTCAAA CGTCAGAAGC TCACCGGAAC TGCAACGAAT 
TGGAGTGATT ATGTTCCGCG ACACGTTGTT GAAAAGATGG AAGAATCGAG AAAAAACCAG 
CTGGAAATTG TTCGAAGGCG ATTTGAGATG ATTCGTGCTC CGATTATTCC ACTGGAAATG 
GTTGCGCTGG TTCGAGAGGA AATTATTGCA GAATTTCCAC GTTTGGCTGT GGAAGAGGAC 
GAGGTTGTGC AGGAGAGGCT TTTGGAGTAT TGCGAGTTGT TGGTGCAAAG ATTCGGAATG 
TACGTCGAAC CAGTGCTGAC CGATGCTTGG CAGTGTCGTC CATCATCGTC TGGTCTTCCA 
TCATATATTC GCAACAATTT ATCAAATATC GAGCTGAATT CTCGTTCTCT TCTCCTCAAC 
ACCTCCACTA ATTTCGATAC CCGAATGTCG ATCTCACGTG CTCTTCAATT CCCAGAACTC 
CGTCTGATCG AGTACGATTG TGGAAAGCTT CAGACGTTGG CTGTTCTGCT TCGTCAGTTG 
TACCTGTACA AGCACAGATG TCTGATCTTC ACGCAAATGT CAAAGATGCT CGACGTTCTG 
CAGACCTTCC TTTCTCATCA CGGTTATCAG TATTTCCGCC TCGACGGTAC CACTGGTGTC 
GAACAAAGAC AGGCGATGAT GGAGCGGTTC AACGCGGATC CCAAGGTGTT TTGCTTCATT 
CTGTCGACGA GATCCGGTGG TGTTGGAGTC AATCTAACCG GTGCTGACAC TGTGATCTTC 
TACGATTCGG ATTGGAATCC GACGATGGAT GCTCAGGCTC AGGATAGATG TCATCGTATC 
GGACAGACGA GGAATGTCTC GATTTATCGA TTGATTTCCG AGCGAACAAT TGAGGAGAAT 
ATTCTGAGAA AGGCAACACA GAAGCGGCGA CTTGGAGAGT TGGCAATTGA CGAGGCTGGC 
TTCACACCCG AGTTCTTCAA ACAATCTGAC AGTATTCGGG ATCTTTTTGA TGGAGAGAAT 
GTGGAAGTGA CTGCTGTGGC AGATGTTGCG ACGACGATGA GCGAGAAAGA AATGGAGGTT 
GCGATGGCAA AGTGTGAAGA TGAAGCTGAT GTGAATGCGG CGAAGATTGC GGTGGCCGAG 
GCGAACGTTG ATAATGCGGA GTTTGATGAG AAATCATTGC CGCCGATGAG CAATTTGCAA 
GGAGATGAGG AGGCTGATGA GAAGTATATG GAGTTGATAC AACAGCTCAA ACCAATCGAA 
CGATATGCCA TTAACTTTCT TGAGACACAG TACAAGCCAG AATTTGAGGA AGAATGCAAA 
GAGGCAGAGG CTCTTATCGA CCAAAAACGC GAAGAATGGG ACAAAAATCT CAACGATACC 
GCCGTCATTG ACCTCGACGA TTCGGATAGT CTGCTGCTCA ACGATCCTTC GACTTCTGCC 
GATTTTTATC AGAGCTCAAG TCTTTTAGAC GAGATAAAAT TCTACGACGA GCTGGACGAT 
ATCATGCCAA TCTGGCTTCC ACCATCACCA CCAGATTCGG ATGCGGATTT CGACTTGAGA 
ATGGAAGATG ATTGTCTCGA TCTGATGTAT GAAATTGAAC AAATGAACGA GGCTCGCCTA 
CCACAAGTTT GTCATGAAAT GAGACGTCCG TTGGCTGAAA AACAGCAGAA ACAGAACACG 
TTGAATGCGT TTAATGACAT TCTATCGGCA AAAGAAAAGG AATCGGTGTA CGATGCGGTC 
AACAAGTGCC TTCAAATGCC ACAATCCGAA GCGATCACAG CAGAATCTGC AGCGTCTCCA 
GCATACACGG AACACTCATC ATTCTCGATG GATGATACAA GCCAGGATGC GAAGATTGAG 
CCAAGTTTGA CTGAAAATCA ACAACCCACC ACCACCGCCA CTACTACTAC TACAGTACCC 
CAACAACAAC AACAACAGCA GCAGCAAAAA TCGTCGAAAA AGAAGAGAAA TGATAATCGA 
ACGGCTCAAA ATCGAACAGC TGAAAATGGT GTGAAACGAG CGACAACTCC ACCACCATCA 
TGGCGTGAAG AGCCAGATTA TGATGGAGCC GAATGGAATA TAGTTGAAGA TTATGCACTA 
CTTCAAGCAG TTCAAGTCGA ATTTGCAAAT GCTCATTTAG TCGAAAAATC GGCGAATGAG 
GGAATGGTGT TGAACTGGGA ATTCGTGTCG AATGCCGTTA ATAAGCAGAC AAGATTTTTC 
CGCTCGGCCC GTCAATGCTC AATTCGATAT CAAATGTTTG TTCGGCCAAA AGAGCTCGGA 
CAGTTGGTGG CTTCTGATCC GATTTCCAAG AAAACGATGA AAGTCGACCT ATCGCATACT 
GAATTATCTC ATTTGAGAAA AGGACGAATG ACTACGGAGA GCCAATATGC TCATGATTAT 
GGAATATTGA CTGATAAGAA ACATGTGAAT AGATTTAAAA GTGTTCGAGT GGCGGCAACA 
CGGAGACCTG TTCAGTTTTG GAGAGGCCCT AAAGCTCTAG AATCTCGAAA TCTGCAATCT 
CTAAACGGCG GAATGCCACC ACGACACGAG TCGAGACTCG CCGAATTCGA CGTAAAAACC 
AATATTCGCC TGGACGCCGA GGACATTGTC ACAATGTCCG ACGAGTCGAT TGTCGCCTAT 
GAAGCGAGCA AGAAGAAGCT ACTGGCCAGT CGTCAAACAA AACCCTCACC ACGTCAAGAT 
GTCCGATTCC ATACGCTGGT TCTTCGGCCG TATACCGTAC CTGTGACAAC TGAGTACTCG 
GCTGCACCTT CTCGTCGTGA AATGCGCATC GCTGTTCCAC CGCTTCAGCC TTCGGCTTTA 
TCTACGATTT CCTCAGTTGC TGCTGCTGCC ACGTCTGGGC CACTACCATC AATTCAGCAT 
TTGCAGTCGT CGTCTACGGG CTTGGGATCT CAGCAAAATT TGCAAAATTC GCATAATTCT 
GAGCAAAGAA ATAATGTGCA AAATATGCAT CAAAATCAAT ATAATTCAAG TCAAAATCCG 
CCAATACCTA TCCGACAAAT CGGAGCAGCA TCATCACACC AACATGATCA AGGATCTCAG 
GGGCCTGGGG GAAAACCACA AGCCTATCAC CTGGTGCAAC AGGGATCACA GCAACAGCAG 
CAGCAGCAGC AGCAGGCGAC GTTACAGCGA AGAAATGCGG CGGCGGCGGC AGGGTCGAAT 
GTGCAGTTTA TTCAGCAGCA GCAGCAGCAG CAGCAATCGG GTAAAAATTG CGGTCAAGGA 
CAAAGCTTCG TTGTCATGGG CTCGCAGAGC TCATCAAATG ATGGACAAGG TGGAGCATCG 
ACCGTCGGAG GAGGAGGAGG AGGATCACAA CAGCCTCACC AGCAGCAGCA GCAGCAGCCA 
CAACAAAGAA TACAGTACAT TCCACAAGTT ACCGGTAGCG GAAATAACGG TGGAGGTGGT 
GGAAGAGGAG GCTACGGTAG TACACTGGTC ATGCCAAGAG GAGGACGTGT TGTCAGGCCA 
GCGGGAACTC TACCAGGCGG TGGACGTCTG TATGTCGATC ATAACCGTCA TCCATATCCA 
ATGTCGTCAA ATGTTGTGCC AGTACGTGTT CTACCAGCCA CGCAACAAGG ACAACAACGA 
ATGATGACAG GACAACGTCG TCCGGCTCCA GCGCCCGGTA CTGTCGCCGC AATGGTGTTG 
CCGAATCGAG GAGCTGGTGG AATTCCGCAA ATGCGCAGTT TGCAACGAGG CAGTTACACC 
GGCGGTGGTG GTCAGCAACG AATCAACGTG ATGGTTCAAC CACAACAAAT GCGCAGCAAC 
AATGGCGGTG GAGTCGGTGG CCAAGGAGGC CTCCAGGGTG GTCCAGGAGG TCCGCAAGGA 
ATTCGTCGGC CACTCGTCGG ACGGCCACTA CAACGAGGAG TCGATAATCA GGCGCCGACG 
GTTGCTCAGG TCGTTGTTGC TCCGCCGCAA GGAATGCAGC AGGCATCACA AGGACCACCC 
GTACTTCATA TGCAGAGAGC GGTTTCCATG CAAATGCCGA CGAGTCATCA TCATCAAGGC 
CAACAGCAGG CTCCTCCGCA GAGCTCACAG CAGGCTTCGC AACAGGCTCC CACATCGGAT 
TCTGGGACGA GTGCTCCGCC ACGACAAGCA CCACCACCAC AAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255179.1
CDS	1..7188
Translation	MPATPVRASSTRISRRTSSRSVADDQPSTSSAVAPPPSPIAIETDEDAVVEEEKKKKKTSDDLEIITPRTPVDRRIPYICSILLTENRSIRDKLVLSSGPVRQEDHEEQIARAQRIQPVVDQIQRVEQIILNGSVEDILKDPRFAVMADLTKEPPPTPAPPPPIQKTMQPIEVKIEDSEGSNTAQPSVLPSCGGGETNVERAAKREAHVLARIAELRKNGLWSNSRLPKCVEPERNKTHWDYLLEEVKWMAVDFRTETNTKRKIAKVIAHAIAKQHRDKQIEIERAAEREIKEKRKMCAGIAKMVRDFWSSTDKVVDIRAKEVLESRLRKARNKHLMFVIGQVDEMSNIVQEGLVSSSKSPSIASDRDDKDEEFKAPGSDSESDDEQTIANAEKSQKKEDVRQEVDALQNEATVDMDDFLYTLPPEYLKAYGLTQEDLEEMKREKLEEQKARKEACGDNEEKMEIDESPSSDAQKPSTSSSDLTAEQLQDPTAEDGNGDGHGVLENVDYVKLNSQDSDERQQELANIAEEALKFQPKGYTLETTQVKTPVPFLIRGQLREYQMVGLDWMVTLYEKNLNGILADEMGLGKTIQTISLLAHMACSESIWGPHLIVVPTSVILNWEMEFKKWCPALKILTYFGTAKERAEKRKGWMKPNCFHVCITSYKTVTQDIRAFKQRAWQYLILDEAQNIKNWKSQRWQALLNVRARRRLLLTGTPLQNSLMELWSLMHFLMPTIFSSHDDFKDWFSNPLTGMMEGNMEFNAPLIGRLHKVLRPFILRRLKKEVEKQLPEKTEHIVNCSLSKRQRYLYDDFMSRRSTKENLKSGNMMSVLNIVMQLRKCCNHPNLFEPRPVVAPFVVEKLQLDVPARLFEISQQDPSSSSASQIPEIFNLSKIGYQSSVRSAKPLIEELEAMSTYPEPRAPEVGGFRFNRTAFVAKNPHTEESEDEGVMRSRVLPKPINGTAQPLQNGNSIPQNAPNRPQTSCIRSKTVVNTVPLTISTDRSGFHFNMANVGRGVVRLDDSARMSPPLKRQKLTGTATNWSDYVPRHVVEKMEESRKNQLEIVRRRFEMIRAPIIPLEMVALVREEIIAEFPRLAVEEDEVVQERLLEYCELLVQRFGMYVEPVLTDAWQCRPSSSGLPSYIRNNLSNIELNSRSLLLNTSTNFDTRMSISRALQFPELRLIEYDCGKLQTLAVLLRQLYLYKHRCLIFTQMSKMLDVLQTFLSHHGYQYFRLDGTTGVEQRQAMMERFNADPKVFCFILSTRSGGVGVNLTGADTVIFYDSDWNPTMDAQAQDRCHRIGQTRNVSIYRLISERTIEENILRKATQKRRLGELAIDEAGFTPEFFKQSDSIRDLFDGENVEVTAVADVATTMSEKEMEVAMAKCEDEADVNAAKIAVAEANVDNAEFDEKSLPPMSNLQGDEEADEKYMELIQQLKPIERYAINFLETQYKPEFEEECKEAEALIDQKREEWDKNLNDTAVIDLDDSDSLLLNDPSTSADFYQSSSLLDEIKFYDELDDIMPIWLPPSPPDSDADFDLRMEDDCLDLMYEIEQMNEARLPQVCHEMRRPLAEKQQKQNTLNAFNDILSAKEKESVYDAVNKCLQMPQSEAITAESAASPAYTEHSSFSMDDTSQDAKIEPSLTENQQPTTTATTTTTVPQQQQQQQQQKSSKKKRNDNRTAQNRTAENGVKRATTPPPSWREEPDYDGAEWNIVEDYALLQAVQVEFANAHLVEKSANEGMVLNWEFVSNAVNKQTRFFRSARQCSIRYQMFVRPKELGQLVASDPISKKTMKVDLSHTELSHLRKGRMTTESQYAHDYGILTDKKHVNRFKSVRVAATRRPVQFWRGPKALESRNLQSLNGGMPPRHESRLAEFDVKTNIRLDAEDIVTMSDESIVAYEASKKKLLASRQTKPSPRQDVRFHTLVLRPYTVPVTTEYSAAPSRREMRIAVPPLQPSALSTISSVAAAATSGPLPSIQHLQSSSTGLGSQQNLQNSHNSEQRNNVQNMHQNQYNSSQNPPIPIRQIGAASSHQHDQGSQGPGGKPQAYHLVQQGSQQQQQQQQQATLQRRNAAAAAGSNVQFIQQQQQQQQSGKNCGQGQSFVVMGSQSSSNDGQGGASTVGGGGGGSQQPHQQQQQQPQQRIQYIPQVTGSGNNGGGGGRGGYGSTLVMPRGGRVVRPAGTLPGGGRLYVDHNRHPYPMSSNVVPVRVLPATQQGQQRMMTGQRRPAPAPGTVAAMVLPNRGAGGIPQMRSLQRGSYTGGGGQQRINVMVQPQQMRSNNGGGVGGQGGLQGGPGGPQGIRRPLVGRPLQRGVDNQAPTVAQVVVAPPQGMQQASQGPPVLHMQRAVSMQMPTSHHHQGQQQAPPQSSQQASQQAPTSDSGTSAPPRQAPPPQN

Target ORF information:

RefSeq Version	NM_001268250.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268250.1

ORF Insert Sequence:

ATGCCGGCAA CACCGGTGCG TGCTTCAAGT ACTCGAATAA GCAGACGTAC ATCATCAAGA 
TCAGTGGCTG ATGATCAGCC ATCAACTTCG TCTGCGGTGG CTCCACCTCC TTCACCCATT 
GCCATAGAAA CTGATGAAGA TGCGGTAGTT GAGGAGGAGA AAAAGAAGAA AAAGACATCA 
GATGATTTGG AAATTATCAC TCCAAGAACT CCAGTCGATC GGCGAATTCC CTACATTTGC 
TCGATTCTTT TGACTGAAAA TCGATCGATT CGCGATAAAT TGGTTCTGAG CAGCGGTCCA 
GTTCGTCAAG AAGATCACGA AGAACAGATT GCTCGAGCTC AACGGATACA GCCAGTTGTC 
GATCAAATTC AACGAGTCGA GCAAATCATA CTCAATGGTT CAGTGGAAGA TATTCTGAAA 
GATCCTCGAT TCGCAGTAAT GGCAGATCTC ACAAAAGAAC CACCACCAAC ACCTGCACCT 
CCTCCTCCAA TCCAGAAGAC AATGCAACCG ATTGAGGTGA AAATTGAGGA TTCAGAGGGC 
TCAAATACGG CTCAACCGAG TGTTCTGCCC AGTTGTGGAG GAGGAGAGAC GAATGTGGAA 
AGAGCCGCCA AAAGAGAAGC GCATGTATTG GCTCGAATCG CCGAGCTCCG TAAGAACGGC 
TTATGGTCGA ACAGTCGTCT GCCAAAGTGC GTCGAACCTG AACGTAATAA AACGCATTGG 
GATTATCTAC TGGAAGAGGT CAAATGGATG GCAGTTGATT TCCGAACCGA GACGAATACG 
AAGCGAAAAA TCGCCAAAGT TATAGCTCAC GCCATTGCGA AACAGCACCG CGACAAGCAG 
ATCGAGATTG AGAGAGCCGC CGAACGGGAG ATCAAGGAGA AGCGAAAAAT GTGTGCAGGA 
ATCGCGAAGA TGGTACGGGA TTTCTGGTCG TCTACGGATA AAGTTGTGGA TATTCGAGCG 
AAGGAAGTTC TGGAGTCGAG GCTCAGGAAG GCGAGAAATA AGCATTTGAT GTTTGTAATT 
GGACAAGTCG ATGAAATGAG CAATATTGTG CAAGAAGGAC TTGTTTCATC GTCGAAATCC 
CCATCAATTG CATCGGATCG AGATGATAAA GATGAAGAAT TCAAAGCACC TGGCTCTGAT 
TCAGAATCTG ACGATGAGCA GACAATTGCA AACGCGGAAA AGTCACAGAA AAAGGAAGAT 
GTTCGACAGG AAGTTGATGC TCTTCAAAAC GAGGCAACTG TGGATATGGA TGACTTTTTG 
TACACTTTAC CGCCGGAATA TCTGAAGGCT TATGGTCTGA CGCAGGAGGA TTTGGAGGAG 
ATGAAGCGCG AGAAATTGGA GGAGCAGAAG GCTCGGAAGG AAGCTTGTGG TGATAATGAG 
GAGAAAATGG AGATTGATGA AAGCCCATCA TCAGATGCTC AAAAGCCTTC CACCTCAAGC 
TCAGATCTCA CCGCCGAGCA GCTTCAAGAT CCAACAGCTG AAGACGGCAA CGGTGATGGT 
CATGGTGTAC TTGAAAACGT GGATTACGTG AAGCTCAACA GTCAGGATAG TGATGAACGA 
CAACAAGAGT TGGCGAATAT CGCAGAAGAA GCGCTGAAAT TCCAGCCAAA AGGATATACA 
CTTGAGACGA CACAAGTCAA GACGCCCGTA CCATTCCTGA TTCGAGGACA ACTGAGAGAA 
TATCAAATGG TTGGATTGGA TTGGATGGTT ACACTTTATG AGAAGAATTT GAATGGAATT 
CTTGCCGACG AGATGGGCCT GGGAAAGACG ATTCAAACGA TTTCCCTGCT GGCTCATATG 
GCTTGTAGTG AATCGATTTG GGGACCACAC TTGATTGTTG TGCCGACGTC TGTCATTCTG 
AATTGGGAGA TGGAGTTCAA GAAATGGTGT CCGGCTCTGA AGATTTTGAC GTATTTTGGT 
ACGGCGAAGG AGCGTGCCGA GAAGCGGAAG GGATGGATGA AGCCGAATTG TTTCCATGTG 
TGCATCACAT CATACAAGAC GGTTACTCAA GATATTAGAG CTTTTAAGCA GAGGGCCTGG 
CAGTACCTAA TTCTCGATGA AGCTCAAAAT ATCAAAAACT GGAAGTCCCA ACGTTGGCAG 
GCTCTTCTGA ATGTCCGTGC TCGACGTCGC CTTCTCCTGA CCGGAACTCC ACTTCAGAAC 
TCTCTAATGG AACTGTGGTC GTTGATGCAT TTTTTGATGC CAACAATATT CTCAAGTCAT 
GATGATTTCA AGGATTGGTT CTCGAATCCG TTGACAGGGA TGATGGAAGG AAATATGGAA 
TTCAATGCTC CACTAATCGG ACGACTTCAC AAAGTGCTCC GTCCGTTTAT TCTGCGGCGG 
CTCAAGAAGG AAGTTGAGAA GCAGCTGCCA GAGAAGACTG AGCATATTGT GAATTGTTCG 
TTGTCAAAGC GGCAGAGATA CCTGTACGAT GACTTTATGA GTCGTAGATC AACAAAGGAG 
AATCTAAAGT CTGGAAATAT GATGTCGGTG CTCAACATTG TGATGCAACT CCGAAAATGT 
TGTAATCATC CGAATCTCTT CGAGCCGCGG CCAGTTGTTG CTCCGTTCGT CGTTGAGAAG 
CTTCAGCTCG ATGTTCCGGC TCGTCTCTTT GAAATTTCGC AGCAAGATCC CTCCTCCTCC 
TCAGCTAGTC AAATTCCGGA AATTTTCAAT TTATCCAAAA TCGGCTATCA ATCTTCCGTT 
CGATCTGCAA AACCACTCAT CGAAGAGCTT GAAGCAATGA GCACTTATCC GGAGCCACGA 
GCACCAGAAG TTGGCGGATT TCGGTTCAAT CGGACGGCTT TTGTTGCAAA GAATCCGCAT 
ACGGAAGAGT CGGAGGACGA AGGTGTTATG AGAAGTCGTG TTCTGCCAAA ACCAATTAAT 
GGAACAGCTC AACCACTTCA AAATGGAAAT TCAATACCAC AAAATGCTCC AAATCGTCCA 
CAAACTTCAT GCATTCGTTC AAAAACCGTC GTAAATACAG TTCCACTGAC CATCTCCACC 
GATCGAAGTG GTTTTCATTT TAATATGGCC AATGTTGGAA GAGGTGTTGT TCGTTTGGAT 
GATTCAGCAC GTATGAGCCC ACCGCTCAAA CGTCAGAAGC TCACCGGAAC TGCAACGAAT 
TGGAGTGATT ATGTTCCGCG ACACGTTGTT GAAAAGATGG AAGAATCGAG AAAAAACCAG 
CTGGAAATTG TTCGAAGGCG ATTTGAGATG ATTCGTGCTC CGATTATTCC ACTGGAAATG 
GTTGCGCTGG TTCGAGAGGA AATTATTGCA GAATTTCCAC GTTTGGCTGT GGAAGAGGAC 
GAGGTTGTGC AGGAGAGGCT TTTGGAGTAT TGCGAGTTGT TGGTGCAAAG ATTCGGAATG 
TACGTCGAAC CAGTGCTGAC CGATGCTTGG CAGTGTCGTC CATCATCGTC TGGTCTTCCA 
TCATATATTC GCAACAATTT ATCAAATATC GAGCTGAATT CTCGTTCTCT TCTCCTCAAC 
ACCTCCACTA ATTTCGATAC CCGAATGTCG ATCTCACGTG CTCTTCAATT CCCAGAACTC 
CGTCTGATCG AGTACGATTG TGGAAAGCTT CAGACGTTGG CTGTTCTGCT TCGTCAGTTG 
TACCTGTACA AGCACAGATG TCTGATCTTC ACGCAAATGT CAAAGATGCT CGACGTTCTG 
CAGACCTTCC TTTCTCATCA CGGTTATCAG TATTTCCGCC TCGACGGTAC CACTGGTGTC 
GAACAAAGAC AGGCGATGAT GGAGCGGTTC AACGCGGATC CCAAGGTGTT TTGCTTCATT 
CTGTCGACGA GATCCGGTGG TGTTGGAGTC AATCTAACCG GTGCTGACAC TGTGATCTTC 
TACGATTCGG ATTGGAATCC GACGATGGAT GCTCAGGCTC AGGATAGATG TCATCGTATC 
GGACAGACGA GGAATGTCTC GATTTATCGA TTGATTTCCG AGCGAACAAT TGAGGAGAAT 
ATTCTGAGAA AGGCAACACA GAAGCGGCGA CTTGGAGAGT TGGCAATTGA CGAGGCTGGC 
TTCACACCCG AGTTCTTCAA ACAATCTGAC AGTATTCGGG ATCTTTTTGA TGGAGAGAAT 
GTGGAAGTGA CTGCTGTGGC AGATGTTGCG ACGACGATGA GCGAGAAAGA AATGGAGGTT 
GCGATGGCAA AGTGTGAAGA TGAAGCTGAT GTGAATGCGG CGAAGATTGC GGTGGCCGAG 
GCGAACGTTG ATAATGCGGA GTTTGATGAG AAATCATTGC CGCCGATGAG CAATTTGCAA 
GGAGATGAGG AGGCTGATGA GAAGTATATG GAGTTGATAC AACAGCTCAA ACCAATCGAA 
CGATATGCCA TTAACTTTCT TGAGACACAG TACAAGCCAG AATTTGAGGA AGAATGCAAA 
GAGGCAGAGG CTCTTATCGA CCAAAAACGC GAAGAATGGG ACAAAAATCT CAACGATACC 
GCCGTCATTG ACCTCGACGA TTCGGATAGT CTGCTGCTCA ACGATCCTTC GACTTCTGCC 
GATTTTTATC AGAGCTCAAG TCTTTTAGAC GAGATAAAAT TCTACGACGA GCTGGACGAT 
ATCATGCCAA TCTGGCTTCC ACCATCACCA CCAGATTCGG ATGCGGATTT CGACTTGAGA 
ATGGAAGATG ATTGTCTCGA TCTGATGTAT GAAATTGAAC AAATGAACGA GGCTCGCCTA 
CCACAAGTTT GTCATGAAAT GAGACGTCCG TTGGCTGAAA AACAGCAGAA ACAGAACACG 
TTGAATGCGT TTAATGACAT TCTATCGGCA AAAGAAAAGG AATCGGTGTA CGATGCGGTC 
AACAAGTGCC TTCAAATGCC ACAATCCGAA GCGATCACAG CAGAATCTGC AGCGTCTCCA 
GCATACACGG AACACTCATC ATTCTCGATG GATGATACAA GCCAGGATGC GAAGATTGAG 
CCAAGTTTGA CTGAAAATCA ACAACCCACC ACCACCGCCA CTACTACTAC TACAGTACCC 
CAACAACAAC AACAACAGCA GCAGCAAAAA TCGTCGAAAA AGAAGAGAAA TGATAATCGA 
ACGGCTCAAA ATCGAACAGC TGAAAATGGT GTGAAACGAG CGACAACTCC ACCACCATCA 
TGGCGTGAAG AGCCAGATTA TGATGGAGCC GAATGGAATA TAGTTGAAGA TTATGCACTA 
CTTCAAGCAG TTCAAGTCGA ATTTGCAAAT GCTCATTTAG TCGAAAAATC GGCGAATGAG 
GGAATGGTGT TGAACTGGGA ATTCGTGTCG AATGCCGTTA ATAAGCAGAC AAGATTTTTC 
CGCTCGGCCC GTCAATGCTC AATTCGATAT CAAATGTTTG TTCGGCCAAA AGAGCTCGGA 
CAGTTGGTGG CTTCTGATCC GATTTCCAAG AAAACGATGA AAGTCGACCT ATCGCATACT 
GAATTATCTC ATTTGAGAAA AGGACGAATG ACTACGGAGA GCCAATATGC TCATGATTAT 
GGAATATTGA CTGATAAGAA ACATGTGAAT AGATTTAAAA GTGTTCGAGT GGCGGCAACA 
CGGAGACCTG TTCAGTTTTG GAGAGGCCCT AAAGCTCTAG AATCTCGAAA TCTGCAATCT 
CTAAACGGCG GAATGCCACC ACGACACGAG TCGAGACTCG CCGAATTCGA CGTAAAAACC 
AATATTCGCC TGGACGCCGA GGACATTGTC ACAATGTCCG ACGAGTCGAT TGTCGCCTAT 
GAAGCGAGCA AGAAGAAGCT ACTGGCCAGT CGTCAAACAA AACCCTCACC ACGTCAAGAT 
GTCCGATTCC ATACGCTGGT TCTTCGGCCG TATACCGTAC CTGTGACAAC TGAGTACTCG 
GCTGCACCTT CTCGTCGTGA AATGCGCATC GCTGTTCCAC CGCTTCAGCC TTCGGCTTTA 
TCTACGATTT CCTCAGTTGC TGCTGCTGCC ACGTCTGGGC CACTACCATC AATTCAGCAT 
TTGCAGTCGT CGTCTACGGG CTTGGGATCT CAGCAAAATT TGCAAAATTC GCATAATTCT 
GAGCAAAGAA ATAATGTGCA AAATATGCAT CAAAATCAAT ATAATTCAAG TCAAAATCCG 
CCAATACCTA TCCGACAAAT CGGAGCAGCA TCATCACACC AACATGATCA AGGATCTCAG 
GGGCCTGGGG GAAAACCACA AGCCTATCAC CTGGTGCAAC AGGGATCACA GCAACAGCAG 
CAGCAGCAGC AGCAGGCGAC GTTACAGCGA AGAAATGCGG CGGCGGCGGC AGGGTCGAAT 
GTGCAGTTTA TTCAGCAGCA GCAGCAGCAG CAGCAATCGG GTAAAAATTG CGGTCAAGGA 
CAAAGCTTCG TTGTCATGGG CTCGCAGAGC TCATCAAATG ATGGACAAGG TGGAGCATCG 
ACCGTCGGAG GAGGAGGAGG AGGATCACAA CAGCCTCACC AGCAGCAGCA GCAGCAGCCA 
CAACAAAGAA TACAGTACAT TCCACAAGTT ACCGGTAGCG GAAATAACGG TGGAGGTGGT 
GGAAGAGGAG GCTACGGTAG TACACTGGTC ATGCCAAGAG GAGGACGTGT TGTCAGGCCA 
GCGGGAACTC TACCAGGCGG TGGACGTCTG TATGTCGATC ATAACCGTCA TCCATATCCA 
ATGTCGTCAA ATGTTGTGCC AGTACGTGTT CTACCAGCCA CGCAACAAGG ACAACAACGA 
ATGATGACAG GACAACGTCG TCCGGCTCCA GCGCCCGGTA CTGTCGCCGC AATGGTGTTG 
CCGAATCGAG GAGCTGGTGG AATTCCGCAA ATGCGCAGTT TGCAACGAGG CAGTTACACC 
GGCGGTGGTG GTCAGCAACG AATCAACGTG ATGGTTCAAC CACAACAAAT GCGCAGCAAC 
AATGGCGGTG GAGTCGGTGG CCAAGGAGGC CTCCAGGGTG GTCCAGGAGG TCCGCAAGGA 
ATTCGTCGGC CACTCGTCGG ACGGCCACTA CAACGAGGAG TCGATAATCA GGCGCCGACG 
GTTGCTCAGG TCGTTGTTGC TCCGCCGCAA GGAATGCAGC AGGCATCACA AGGACCACCC 
GTACTTCATA TGCAGAGAGC GGTTTCCATG CAAATGCCGA CGAGTCATCA TCATCAAGGC 
CAACAGCAGG CTCCTCCGCA GAGCTCACAG CAGGCTTCGC AACAGGCTCC CACATCGGAT 
TCTGGGACGA GTGCTCCGCC ACGACAAGCA CCACCACCAC AAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssl-1 ( NM_001268251.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255180.1 Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.

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CloneID	OCa340602
Clone ID Related Accession (Same CDS sequence)	NM_001268251.2 , NM_001268251.1
Accession Version	NM_001268251.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Helicase ssl-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). COMPLETENESS: incomplete on both ends.

ATGCCGGCAA CACCGGTGCG TGCTTCAAGT ACTCGAATAA GCAGACGTAC ATCATCAAGA 
TCAGTGGCTG ATGATCAGCC ATCAACTTCG TCTGCGGTGG CTCCACCTCC TTCACCCATT 
GCCATAGAAA CTGATGAAGA TGCGGTAGTT GAGGAGGAGA AAAAGAAGAA AAAGACATCA 
GATGATTTGG AAATTATCAC TCCAAGAACT CCAGTCGATC GGCGAATTCC CTACATTTGC 
TCGATTCTTT TGACTGAAAA TCGATCGATT CGCGATAAAT TGGTTCTGAG CAGCGGTCCA 
GTTCGTCAAG AAGATCACGA AGAACAGATT GCTCGAGCTC AACGGATACA GCCAGTTGTC 
GATCAAATTC AACGAGTCGA GCAAATCATA CTCAATGGTT CAGTGGAAGA TATTCTGAAA 
GATCCTCGAT TCGCAGTAAT GGCAGATCTC ACAAAAGAAC CACCACCAAC ACCTGCACCT 
CCTCCTCCAA TCCAGAAGAC AATGCAACCG ATTGAGGTGA AAATTGAGGA TTCAGAGGGC 
TCAAATACGG CTCAACCGAG TGTTCTGCCC AGTTGTGGAG GAGGAGAGAC GAATGTGGAA 
AGAGCCGCCA AAAGAGAAGC GCATGTATTG GCTCGAATCG CCGAGCTCCG TAAGAACGGC 
TTATGGTCGA ACAGTCGTCT GCCAAAGTGC GTCGAACCTG AACGTAATAA AACGCATTGG 
GATTATCTAC TGGAAGAGGT CAAATGGATG GCAGTTGATT TCCGAACCGA GACGAATACG 
AAGCGAAAAA TCGCCAAAGT TATAGCTCAC GCCATTGCGA AACAGCACCG CGACAAGCAG 
ATCGAGATTG AGAGAGCCGC CGAACGGGAG ATCAAGGAGA AGCGAAAAAT GTGTGCAGGA 
ATCGCGAAGA TGGTACGGGA TTTCTGGTCG TCTACGGATA AAGTTGTGGA TATTCGAGCG 
AAGGAAGTTC TGGAGTCGAG GCTCAGGAAG GCGAGAAATA AGCATTTGAT GTTTGTAATT 
GGACAAGTCG ATGAAATGAG CAATATTGTG CAAGAAGGAC TTGTTTCATC GTCGAAATCC 
CCATCAATTG CATCGGATCG AGATGATAAA GATGAAGAAT TCAAAGCACC TGGCTCTGAT 
TCAGAATCTG ACGATGAGCA GACAATTGCA AACGCGGAAA AGTCACAGAA AAAGGAAGAT 
GTTCGACAGG AAGTTGATGC TCTTCAAAAC GAGGCAACTG TGGATATGGA TGACTTTTTG 
TACACTTTAC CGCCGGAATA TCTGAAGGCT TATGGTCTGA CGCAGGAGGA TTTGGAGGAG 
ATGAAGCGCG AGAAATTGGA GGAGCAGAAG GCTCGGAAGG AAGCTTGTGG TGATAATGAG 
GAGAAAATGG AGATTGATGA AAGCCCATCA TCAGATGCTC AAAAGCCTTC CACCTCAAGC 
TCAGATCTCA CCGCCGAGCA GCTTCAAGAT CCAACAGCTG AAGACGGCAA CGGTGATGGT 
CATGGTGTAC TTGAAAACGT GGATTACGTG AAGCTCAACA GTCAGGATAG TGATGAACGA 
CAACAAGAGT TGGCGAATAT CGCAGAAGAA GCGCTGAAAT TCCAGCCAAA AGGATATACA 
CTTGAGACGA CACAAGTCAA GACGCCCGTA CCATTCCTGA TTCGAGGACA ACTGAGAGAA 
TATCAAATGG TTGGATTGGA TTGGATGGTT ACACTTTATG AGAAGAATTT GAATGGAATT 
CTTGCCGACG AGATGGGCCT GGGAAAGACG ATTCAAACGA TTTCCCTGCT GGCTCATATG 
GCTTGTAGTG AATCGATTTG GGGACCACAC TTGATTGTTG TGCCGACGTC TGTCATTCTG 
AATTGGGAGA TGGAGTTCAA GAAATGGTGT CCGGCTCTGA AGATTTTGAC GTATTTTGGT 
ACGGCGAAGG AGCGTGCCGA GAAGCGGAAG GGATGGATGA AGCCGAATTG TTTCCATGTG 
TGCATCACAT CATACAAGAC GGTTACTCAA GATATTAGAG CTTTTAAGCA GAGGGCCTGG 
CAGTACCTAA TTCTCGATGA AGCTCAAAAT ATCAAAAACT GGAAGTCCCA ACGTTGGCAG 
GCTCTTCTGA ATGTCCGTGC TCGACGTCGC CTTCTCCTGA CCGGAACTCC ACTTCAGAAC 
TCTCTAATGG AACTGTGGTC GTTGATGCAT TTTTTGATGC CAACAATATT CTCAAGTCAT 
GATGATTTCA AGGATTGGTT CTCGAATCCG TTGACAGGGA TGATGGAAGG AAATATGGAA 
TTCAATGCTC CACTAATCGG ACGACTTCAC AAAGTGCTCC GTCCGTTTAT TCTGCGGCGG 
CTCAAGAAGG AAGTTGAGAA GCAGCTGCCA GAGAAGACTG AGCATATTGT GAATTGTTCG 
TTGTCAAAGC GGCAGAGATA CCTGTACGAT GACTTTATGA GTCGTAGATC AACAAAGGAG 
AATCTAAAGT CTGGAAATAT GATGTCGGTG CTCAACATTG TGATGCAACT CCGAAAATGT 
TGTAATCATC CGAATCTCTT CGAGCCGCGG CCAGTTGTTG CTCCGTTCGT CGTTGAGAAG 
CTTCAGCTCG ATGTTCCGGC TCGTCTCTTT GAAATTTCGC AGCAAGATCC CTCCTCCTCC 
TCAGCTAGTC AAATTCCGGA AATTTTCAAT TTATCCAAAA TCGGCTATCA ATCTTCCGTT 
CGATCTGCAA AACCACTCAT CGAAGAGCTT GAAGCAATGA GCACTTATCC GGAGCCACGA 
GCACCAGAAG TTGGCGGATT TCGGTTCAAT CGGACGGCTT TTGTTGCAAA GAATCCGCAT 
ACGGAAGAGT CGGAGGACGA AGGTGTTATG AGAAGTCGTG TTCTGCCAAA ACCAATTAAT 
GGAACAGCTC AACCACTTCA AAATGGAAAT TCAATACCAC AAAATGCTCC AAATCGTCCA 
CAAACTTCAT GCATTCGTTC AAAAACCGTC GTAAATACAG TTCCACTGAC CATCTCCACC 
GATCGAAGTG GTTTTCATTT TAATATGGCC AATGTTGGAA GAGGTGTTGT TCGTTTGGAT 
GATTCAGCAC GTATGAGCCC ACCGCTCAAA CGTCAGAAGC TCACCGGAAC TGCAACGAAT 
TGGAGTGATT ATGTTCCGCG ACACGTTGTT GAAAAGATGG AAGAATCGAG AAAAAACCAG 
CTGGAAATTG TTCGAAGGCG ATTTGAGATG ATTCGTGCTC CGATTATTCC ACTGGAAATG 
GTTGCGCTGG TTCGAGAGGA AATTATTGCA GAATTTCCAC GTTTGGCTGT GGAAGAGGAC 
GAGGTTGTGC AGGAGAGGCT TTTGGAGTAT TGCGAGTTGT TGGTGCAAAG ATTCGGAATG 
TACGTCGAAC CAGTGCTGAC CGATGCTTGG CAGTGTCGTC CATCATCGTC TGGTCTTCCA 
TCATATATTC GCAACAATTT ATCAAATATC GAGCTGAATT CTCGTTCTCT TCTCCTCAAC 
ACCTCCACTA ATTTCGATAC CCGAATGTCG ATCTCACGTG CTCTTCAATT CCCAGAACTC 
CGTCTGATCG AGTACGATTG TGGAAAGCTT CAGACGTTGG CTGTTCTGCT TCGTCAGTTG 
TACCTGTACA AGCACAGATG TCTGATCTTC ACGCAAATGT CAAAGATGCT CGACGTTCTG 
CAGACCTTCC TTTCTCATCA CGGTTATCAG TATTTCCGCC TCGACGGTAC CACTGGTGTC 
GAACAAAGAC AGGCGATGAT GGAGCGGTTC AACGCGGATC CCAAGGTGTT TTGCTTCATT 
CTGTCGACGA GATCCGGTGG TGTTGGAGTC AATCTAACCG GTGCTGACAC TGTGATCTTC 
TACGATTCGG ATTGGAATCC GACGATGGAT GCTCAGGCTC AGGATAGATG TCATCGTATC 
GGACAGACGA GGAATGTCTC GATTTATCGA TTGATTTCCG AGCGAACAAT TGAGGAGAAT 
ATTCTGAGAA AGGCAACACA GAAGCGGCGA CTTGGAGAGT TGGCAATTGA CGAGGCTGGC 
TTCACACCCG AGTTCTTCAA ACAATCTGAC AGTATTCGGG ATCTTTTTGA TGGAGAGAAT 
GTGGAAGTGA CTGCTGTGGC AGATGTTGCG ACGACGATGA GCGAGAAAGA AATGGAGGTT 
GCGATGGCAA AGTGTGAAGA TGAAGCTGAT GTGAATGCGG CGAAGATTGC GGTGGCCGAG 
GCGAACGTTG ATAATGCGGA GTTTGATGAG AAATCATTGC CGCCGATGAG CAATTTGCAA 
GGAGATGAGG AGGCTGATGA GAAGTATATG GAGTTGATAC AACAGCTCAA ACCAATCGAA 
CGATATGCCA TTAACTTTCT TGAGACACAG TACAAGCCAG AATTTGAGGA AGAATGCAAA 
GAGGCAGAGG CTCTTATCGA CCAAAAACGC GAAGAATGGG ACAAAAATCT CAACGATACC 
GCCGTCATTG ACCTCGACGA TTCGGATAGT CTGCTGCTCA ACGATCCTTC GACTTCTGCC 
GATTTTTATC AGAGCTCAAG TCTTTTAGAC GAGGTACGCG ATCGTCGTCG TCGCAGCAGC 
AGCCTTCTCC AAAAAGCCGC TCAAAAACCG GCAAAAAAGC CTCAAAACTT CCAAATTCGT 
GCTCGCTCCC CGTCTAAGCG TAAATCTCAG GCTCCTTCCT TCGATCCATA TGTTTCGTAC 
GCACCGCACG CGCTCGCTTC TCCCCCGGAT TCCCCGCGTA AGAGAAGATC ACGTGGTGCG 
CGTAGTTTAG GTAGTGGTGG TGGTGGTGGT GGTGGTAGTA GATCTGTTGG AAGACCTGCC 
CGCCGATCAG TGAAGAAAGA AGAATCAGAT GATGATGATG AGGATTATTG CCAAGAAGAG 
GAAGTGAAGC GAAATCCGGC AGAAAAGGTC CCGCCGAAAA GAAAACGAGT TGTGTTTGTG 
GAACCTCCAG AGGTGAAGCC GCCGGAGCCG AAAAAACGAG TTGTTGTTCC TGCTCCATCA 
TCATCATCAT CAGCTCTAAC TACTCTTCCA CAACAAGGAC CGCTGATTTC GTTGCCAAAA 
GCTGTGCCAG TTGTACCTCG GCCCCAACAA CAAGCACCAC CACAGCTCAT CAAAAAGCAC 
CAGCAGACTC TGATGCCTGT GAAGGTGCTC AAGATTAGTG GTGGTGGTGG TGGTACTCCA 
GGACCATCCA GTGTATCGCC AGGTCCATCA ATCCTCCGAA GAACCGTTGT TCCAGGCATA 
GGCGCTGGTG GTGTTGGACG CCTACCGCTT GTCAGAATGC CTGTTCGCCC TCCATTTCCT 
GGCTCGCAAG CTCCTGCTCC ACCGCTGAGA AGTGGTGTTG CTCCAACAGC TCCTGCAGCA 
GCTCCACGCC AGTTCGTCGT TCCGTCGTCG AGAGTTCGAG TTATCACGAC GAGAACTCCG 
GTCGCCACCA CCATGGTGCA ACAACAACAA AGCCCGAGCC CGTTGATGTT TCCAGTCCGG 
GTTGTGCAAA GGCCCGGGCC ATCTGGACCA CCACCACCTG GACCTCCAGA TCGCCCAGGA 
TTTGGAATCT ATGAGAAGCC GAGATTCTCA CTTGGATCAC GAAGAAGCCG TGGAGATTCG 
GGCCCGGAAG ATCCGGCGCC ACCACAGCCA CCACCACCCA CCACTTCTAG GCCACCGCCA 
CAAGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255180.1
CDS	1..5649
Translation	MPATPVRASSTRISRRTSSRSVADDQPSTSSAVAPPPSPIAIETDEDAVVEEEKKKKKTSDDLEIITPRTPVDRRIPYICSILLTENRSIRDKLVLSSGPVRQEDHEEQIARAQRIQPVVDQIQRVEQIILNGSVEDILKDPRFAVMADLTKEPPPTPAPPPPIQKTMQPIEVKIEDSEGSNTAQPSVLPSCGGGETNVERAAKREAHVLARIAELRKNGLWSNSRLPKCVEPERNKTHWDYLLEEVKWMAVDFRTETNTKRKIAKVIAHAIAKQHRDKQIEIERAAEREIKEKRKMCAGIAKMVRDFWSSTDKVVDIRAKEVLESRLRKARNKHLMFVIGQVDEMSNIVQEGLVSSSKSPSIASDRDDKDEEFKAPGSDSESDDEQTIANAEKSQKKEDVRQEVDALQNEATVDMDDFLYTLPPEYLKAYGLTQEDLEEMKREKLEEQKARKEACGDNEEKMEIDESPSSDAQKPSTSSSDLTAEQLQDPTAEDGNGDGHGVLENVDYVKLNSQDSDERQQELANIAEEALKFQPKGYTLETTQVKTPVPFLIRGQLREYQMVGLDWMVTLYEKNLNGILADEMGLGKTIQTISLLAHMACSESIWGPHLIVVPTSVILNWEMEFKKWCPALKILTYFGTAKERAEKRKGWMKPNCFHVCITSYKTVTQDIRAFKQRAWQYLILDEAQNIKNWKSQRWQALLNVRARRRLLLTGTPLQNSLMELWSLMHFLMPTIFSSHDDFKDWFSNPLTGMMEGNMEFNAPLIGRLHKVLRPFILRRLKKEVEKQLPEKTEHIVNCSLSKRQRYLYDDFMSRRSTKENLKSGNMMSVLNIVMQLRKCCNHPNLFEPRPVVAPFVVEKLQLDVPARLFEISQQDPSSSSASQIPEIFNLSKIGYQSSVRSAKPLIEELEAMSTYPEPRAPEVGGFRFNRTAFVAKNPHTEESEDEGVMRSRVLPKPINGTAQPLQNGNSIPQNAPNRPQTSCIRSKTVVNTVPLTISTDRSGFHFNMANVGRGVVRLDDSARMSPPLKRQKLTGTATNWSDYVPRHVVEKMEESRKNQLEIVRRRFEMIRAPIIPLEMVALVREEIIAEFPRLAVEEDEVVQERLLEYCELLVQRFGMYVEPVLTDAWQCRPSSSGLPSYIRNNLSNIELNSRSLLLNTSTNFDTRMSISRALQFPELRLIEYDCGKLQTLAVLLRQLYLYKHRCLIFTQMSKMLDVLQTFLSHHGYQYFRLDGTTGVEQRQAMMERFNADPKVFCFILSTRSGGVGVNLTGADTVIFYDSDWNPTMDAQAQDRCHRIGQTRNVSIYRLISERTIEENILRKATQKRRLGELAIDEAGFTPEFFKQSDSIRDLFDGENVEVTAVADVATTMSEKEMEVAMAKCEDEADVNAAKIAVAEANVDNAEFDEKSLPPMSNLQGDEEADEKYMELIQQLKPIERYAINFLETQYKPEFEEECKEAEALIDQKREEWDKNLNDTAVIDLDDSDSLLLNDPSTSADFYQSSSLLDEVRDRRRRSSSLLQKAAQKPAKKPQNFQIRARSPSKRKSQAPSFDPYVSYAPHALASPPDSPRKRRSRGARSLGSGGGGGGGSRSVGRPARRSVKKEESDDDDEDYCQEEEVKRNPAEKVPPKRKRVVFVEPPEVKPPEPKKRVVVPAPSSSSSALTTLPQQGPLISLPKAVPVVPRPQQQAPPQLIKKHQQTLMPVKVLKISGGGGGTPGPSSVSPGPSILRRTVVPGIGAGGVGRLPLVRMPVRPPFPGSQAPAPPLRSGVAPTAPAAAPRQFVVPSSRVRVITTRTPVATTMVQQQQSPSPLMFPVRVVQRPGPSGPPPPGPPDRPGFGIYEKPRFSLGSRRSRGDSGPEDPAPPQPPPPTTSRPPPQA

Target ORF information:

RefSeq Version	NM_001268251.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268251.1

ORF Insert Sequence:

ATGCCGGCAA CACCGGTGCG TGCTTCAAGT ACTCGAATAA GCAGACGTAC ATCATCAAGA 
TCAGTGGCTG ATGATCAGCC ATCAACTTCG TCTGCGGTGG CTCCACCTCC TTCACCCATT 
GCCATAGAAA CTGATGAAGA TGCGGTAGTT GAGGAGGAGA AAAAGAAGAA AAAGACATCA 
GATGATTTGG AAATTATCAC TCCAAGAACT CCAGTCGATC GGCGAATTCC CTACATTTGC 
TCGATTCTTT TGACTGAAAA TCGATCGATT CGCGATAAAT TGGTTCTGAG CAGCGGTCCA 
GTTCGTCAAG AAGATCACGA AGAACAGATT GCTCGAGCTC AACGGATACA GCCAGTTGTC 
GATCAAATTC AACGAGTCGA GCAAATCATA CTCAATGGTT CAGTGGAAGA TATTCTGAAA 
GATCCTCGAT TCGCAGTAAT GGCAGATCTC ACAAAAGAAC CACCACCAAC ACCTGCACCT 
CCTCCTCCAA TCCAGAAGAC AATGCAACCG ATTGAGGTGA AAATTGAGGA TTCAGAGGGC 
TCAAATACGG CTCAACCGAG TGTTCTGCCC AGTTGTGGAG GAGGAGAGAC GAATGTGGAA 
AGAGCCGCCA AAAGAGAAGC GCATGTATTG GCTCGAATCG CCGAGCTCCG TAAGAACGGC 
TTATGGTCGA ACAGTCGTCT GCCAAAGTGC GTCGAACCTG AACGTAATAA AACGCATTGG 
GATTATCTAC TGGAAGAGGT CAAATGGATG GCAGTTGATT TCCGAACCGA GACGAATACG 
AAGCGAAAAA TCGCCAAAGT TATAGCTCAC GCCATTGCGA AACAGCACCG CGACAAGCAG 
ATCGAGATTG AGAGAGCCGC CGAACGGGAG ATCAAGGAGA AGCGAAAAAT GTGTGCAGGA 
ATCGCGAAGA TGGTACGGGA TTTCTGGTCG TCTACGGATA AAGTTGTGGA TATTCGAGCG 
AAGGAAGTTC TGGAGTCGAG GCTCAGGAAG GCGAGAAATA AGCATTTGAT GTTTGTAATT 
GGACAAGTCG ATGAAATGAG CAATATTGTG CAAGAAGGAC TTGTTTCATC GTCGAAATCC 
CCATCAATTG CATCGGATCG AGATGATAAA GATGAAGAAT TCAAAGCACC TGGCTCTGAT 
TCAGAATCTG ACGATGAGCA GACAATTGCA AACGCGGAAA AGTCACAGAA AAAGGAAGAT 
GTTCGACAGG AAGTTGATGC TCTTCAAAAC GAGGCAACTG TGGATATGGA TGACTTTTTG 
TACACTTTAC CGCCGGAATA TCTGAAGGCT TATGGTCTGA CGCAGGAGGA TTTGGAGGAG 
ATGAAGCGCG AGAAATTGGA GGAGCAGAAG GCTCGGAAGG AAGCTTGTGG TGATAATGAG 
GAGAAAATGG AGATTGATGA AAGCCCATCA TCAGATGCTC AAAAGCCTTC CACCTCAAGC 
TCAGATCTCA CCGCCGAGCA GCTTCAAGAT CCAACAGCTG AAGACGGCAA CGGTGATGGT 
CATGGTGTAC TTGAAAACGT GGATTACGTG AAGCTCAACA GTCAGGATAG TGATGAACGA 
CAACAAGAGT TGGCGAATAT CGCAGAAGAA GCGCTGAAAT TCCAGCCAAA AGGATATACA 
CTTGAGACGA CACAAGTCAA GACGCCCGTA CCATTCCTGA TTCGAGGACA ACTGAGAGAA 
TATCAAATGG TTGGATTGGA TTGGATGGTT ACACTTTATG AGAAGAATTT GAATGGAATT 
CTTGCCGACG AGATGGGCCT GGGAAAGACG ATTCAAACGA TTTCCCTGCT GGCTCATATG 
GCTTGTAGTG AATCGATTTG GGGACCACAC TTGATTGTTG TGCCGACGTC TGTCATTCTG 
AATTGGGAGA TGGAGTTCAA GAAATGGTGT CCGGCTCTGA AGATTTTGAC GTATTTTGGT 
ACGGCGAAGG AGCGTGCCGA GAAGCGGAAG GGATGGATGA AGCCGAATTG TTTCCATGTG 
TGCATCACAT CATACAAGAC GGTTACTCAA GATATTAGAG CTTTTAAGCA GAGGGCCTGG 
CAGTACCTAA TTCTCGATGA AGCTCAAAAT ATCAAAAACT GGAAGTCCCA ACGTTGGCAG 
GCTCTTCTGA ATGTCCGTGC TCGACGTCGC CTTCTCCTGA CCGGAACTCC ACTTCAGAAC 
TCTCTAATGG AACTGTGGTC GTTGATGCAT TTTTTGATGC CAACAATATT CTCAAGTCAT 
GATGATTTCA AGGATTGGTT CTCGAATCCG TTGACAGGGA TGATGGAAGG AAATATGGAA 
TTCAATGCTC CACTAATCGG ACGACTTCAC AAAGTGCTCC GTCCGTTTAT TCTGCGGCGG 
CTCAAGAAGG AAGTTGAGAA GCAGCTGCCA GAGAAGACTG AGCATATTGT GAATTGTTCG 
TTGTCAAAGC GGCAGAGATA CCTGTACGAT GACTTTATGA GTCGTAGATC AACAAAGGAG 
AATCTAAAGT CTGGAAATAT GATGTCGGTG CTCAACATTG TGATGCAACT CCGAAAATGT 
TGTAATCATC CGAATCTCTT CGAGCCGCGG CCAGTTGTTG CTCCGTTCGT CGTTGAGAAG 
CTTCAGCTCG ATGTTCCGGC TCGTCTCTTT GAAATTTCGC AGCAAGATCC CTCCTCCTCC 
TCAGCTAGTC AAATTCCGGA AATTTTCAAT TTATCCAAAA TCGGCTATCA ATCTTCCGTT 
CGATCTGCAA AACCACTCAT CGAAGAGCTT GAAGCAATGA GCACTTATCC GGAGCCACGA 
GCACCAGAAG TTGGCGGATT TCGGTTCAAT CGGACGGCTT TTGTTGCAAA GAATCCGCAT 
ACGGAAGAGT CGGAGGACGA AGGTGTTATG AGAAGTCGTG TTCTGCCAAA ACCAATTAAT 
GGAACAGCTC AACCACTTCA AAATGGAAAT TCAATACCAC AAAATGCTCC AAATCGTCCA 
CAAACTTCAT GCATTCGTTC AAAAACCGTC GTAAATACAG TTCCACTGAC CATCTCCACC 
GATCGAAGTG GTTTTCATTT TAATATGGCC AATGTTGGAA GAGGTGTTGT TCGTTTGGAT 
GATTCAGCAC GTATGAGCCC ACCGCTCAAA CGTCAGAAGC TCACCGGAAC TGCAACGAAT 
TGGAGTGATT ATGTTCCGCG ACACGTTGTT GAAAAGATGG AAGAATCGAG AAAAAACCAG 
CTGGAAATTG TTCGAAGGCG ATTTGAGATG ATTCGTGCTC CGATTATTCC ACTGGAAATG 
GTTGCGCTGG TTCGAGAGGA AATTATTGCA GAATTTCCAC GTTTGGCTGT GGAAGAGGAC 
GAGGTTGTGC AGGAGAGGCT TTTGGAGTAT TGCGAGTTGT TGGTGCAAAG ATTCGGAATG 
TACGTCGAAC CAGTGCTGAC CGATGCTTGG CAGTGTCGTC CATCATCGTC TGGTCTTCCA 
TCATATATTC GCAACAATTT ATCAAATATC GAGCTGAATT CTCGTTCTCT TCTCCTCAAC 
ACCTCCACTA ATTTCGATAC CCGAATGTCG ATCTCACGTG CTCTTCAATT CCCAGAACTC 
CGTCTGATCG AGTACGATTG TGGAAAGCTT CAGACGTTGG CTGTTCTGCT TCGTCAGTTG 
TACCTGTACA AGCACAGATG TCTGATCTTC ACGCAAATGT CAAAGATGCT CGACGTTCTG 
CAGACCTTCC TTTCTCATCA CGGTTATCAG TATTTCCGCC TCGACGGTAC CACTGGTGTC 
GAACAAAGAC AGGCGATGAT GGAGCGGTTC AACGCGGATC CCAAGGTGTT TTGCTTCATT 
CTGTCGACGA GATCCGGTGG TGTTGGAGTC AATCTAACCG GTGCTGACAC TGTGATCTTC 
TACGATTCGG ATTGGAATCC GACGATGGAT GCTCAGGCTC AGGATAGATG TCATCGTATC 
GGACAGACGA GGAATGTCTC GATTTATCGA TTGATTTCCG AGCGAACAAT TGAGGAGAAT 
ATTCTGAGAA AGGCAACACA GAAGCGGCGA CTTGGAGAGT TGGCAATTGA CGAGGCTGGC 
TTCACACCCG AGTTCTTCAA ACAATCTGAC AGTATTCGGG ATCTTTTTGA TGGAGAGAAT 
GTGGAAGTGA CTGCTGTGGC AGATGTTGCG ACGACGATGA GCGAGAAAGA AATGGAGGTT 
GCGATGGCAA AGTGTGAAGA TGAAGCTGAT GTGAATGCGG CGAAGATTGC GGTGGCCGAG 
GCGAACGTTG ATAATGCGGA GTTTGATGAG AAATCATTGC CGCCGATGAG CAATTTGCAA 
GGAGATGAGG AGGCTGATGA GAAGTATATG GAGTTGATAC AACAGCTCAA ACCAATCGAA 
CGATATGCCA TTAACTTTCT TGAGACACAG TACAAGCCAG AATTTGAGGA AGAATGCAAA 
GAGGCAGAGG CTCTTATCGA CCAAAAACGC GAAGAATGGG ACAAAAATCT CAACGATACC 
GCCGTCATTG ACCTCGACGA TTCGGATAGT CTGCTGCTCA ACGATCCTTC GACTTCTGCC 
GATTTTTATC AGAGCTCAAG TCTTTTAGAC GAGGTACGCG ATCGTCGTCG TCGCAGCAGC 
AGCCTTCTCC AAAAAGCCGC TCAAAAACCG GCAAAAAAGC CTCAAAACTT CCAAATTCGT 
GCTCGCTCCC CGTCTAAGCG TAAATCTCAG GCTCCTTCCT TCGATCCATA TGTTTCGTAC 
GCACCGCACG CGCTCGCTTC TCCCCCGGAT TCCCCGCGTA AGAGAAGATC ACGTGGTGCG 
CGTAGTTTAG GTAGTGGTGG TGGTGGTGGT GGTGGTAGTA GATCTGTTGG AAGACCTGCC 
CGCCGATCAG TGAAGAAAGA AGAATCAGAT GATGATGATG AGGATTATTG CCAAGAAGAG 
GAAGTGAAGC GAAATCCGGC AGAAAAGGTC CCGCCGAAAA GAAAACGAGT TGTGTTTGTG 
GAACCTCCAG AGGTGAAGCC GCCGGAGCCG AAAAAACGAG TTGTTGTTCC TGCTCCATCA 
TCATCATCAT CAGCTCTAAC TACTCTTCCA CAACAAGGAC CGCTGATTTC GTTGCCAAAA 
GCTGTGCCAG TTGTACCTCG GCCCCAACAA CAAGCACCAC CACAGCTCAT CAAAAAGCAC 
CAGCAGACTC TGATGCCTGT GAAGGTGCTC AAGATTAGTG GTGGTGGTGG TGGTACTCCA 
GGACCATCCA GTGTATCGCC AGGTCCATCA ATCCTCCGAA GAACCGTTGT TCCAGGCATA 
GGCGCTGGTG GTGTTGGACG CCTACCGCTT GTCAGAATGC CTGTTCGCCC TCCATTTCCT 
GGCTCGCAAG CTCCTGCTCC ACCGCTGAGA AGTGGTGTTG CTCCAACAGC TCCTGCAGCA 
GCTCCACGCC AGTTCGTCGT TCCGTCGTCG AGAGTTCGAG TTATCACGAC GAGAACTCCG 
GTCGCCACCA CCATGGTGCA ACAACAACAA AGCCCGAGCC CGTTGATGTT TCCAGTCCGG 
GTTGTGCAAA GGCCCGGGCC ATCTGGACCA CCACCACCTG GACCTCCAGA TCGCCCAGGA 
TTTGGAATCT ATGAGAAGCC GAGATTCTCA CTTGGATCAC GAAGAAGCCG TGGAGATTCG 
GGCCCGGAAG ATCCGGCGCC ACCACAGCCA CCACCACCCA CCACTTCTAG GCCACCGCCA 
CAAGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssl-1 ( NM_001268253.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255182.1 Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.

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CloneID	OCa340603
Clone ID Related Accession (Same CDS sequence)	NM_001268253.2 , NM_001268253.1
Accession Version	NM_001268253.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Helicase ssl-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). COMPLETENESS: incomplete on both ends.

ATGCCAATCT GGCTTCCACC ATCACCACCA GATTCGGATG CGGATTTCGA CTTGAGAATG 
GAAGATGATT GTCTCGATCT GATGTATGAA ATTGAACAAA TGAACGAGGC TCGCCTACCA 
CAAGTTTGTC ATGAAATGAG ACGTCCGTTG GCTGAAAAAC AGCAGAAACA GAACACGTTG 
AATGCGTTTA ATGACATTCT ATCGGCAAAA GAAAAGGAAT CGGTGTACGA TGCGGTCAAC 
AAGTGCCTTC AAATGCCACA ATCCGAAGCG ATCACAGCAG AATCTGCAGC GTCTCCAGCA 
TACACGGAAC ACTCATCATT CTCGATGGAT GATACAAGCC AGGATGCGAA GATTGAGCCA 
AGTTTGACTG AAAATCAACA ACCCACCACC ACCGCCACTA CTACTACTAC AGTACCCCAA 
CAACAACAAC AACAGCAGCA GCAAAAATCG TCGAAAAAGA AGAGAAATGA TAATCGAACG 
GCTCAAAATC GAACAGCTGA AAATGGTGTG AAACGAGCGA CAACTCCACC ACCATCATGG 
CGTGAAGAGC CAGATTATGA TGGAGCCGAA TGGAATATAG TTGAAGATTA TGCACTACTT 
CAAGCAGTTC AAGTCGAATT TGCAAATGCT CATTTAGTCG AAAAATCGGC GAATGAGGGA 
ATGGTGTTGA ACTGGGAATT CGTGTCGAAT GCCGTTAATA AGCAGACAAG ATTTTTCCGC 
TCGGCCCGTC AATGCTCAAT TCGATATCAA ATGTTTGTTC GGCCAAAAGA GCTCGGACAG 
TTGGTGGCTT CTGATCCGAT TTCCAAGAAA ACGATGAAAG TCGACCTATC GCATACTGAA 
TTATCTCATT TGAGAAAAGG ACGAATGACT ACGGAGAGCC AATATGCTCA TGATTATGGA 
ATATTGACTG ATAAGAAACA TGTGAATAGA TTTAAAAGTG TTCGAGTGGC GGCAACACGG 
AGACCTGTTC AGTTTTGGAG AGGCCCTAAA GCTCTAGAAT CTCGAAATCT GCAATCTCTA 
AACGGCGGAA TGCCACCACG ACACGAGTCG AGACTCGCCG AATTCGACGT AAAAACCAAT 
ATTCGCCTGG ACGCCGAGGA CATTGTCACA ATGTCCGACG AGTCGATTGT CGCCTATGAA 
GCGAGCAAGA AGAAGCTACT GGCCAGTCGT CAAACAAAAC CCTCACCACG TCAAGATGTC 
CGATTCCATA CGCTGGTTCT TCGGCCGTAT ACCGTACCTG TGACAACTGA GTACTCGGCT 
GCACCTTCTC GTCGTGAAAT GCGCATCGCT GTTCCACCGC TTCAGCCTTC GGCTTTATCT 
ACGATTTCCT CAGTTGCTGC TGCTGCCACG TCTGGGCCAC TACCATCAAT TCAGCATTTG 
CAGTCGTCGT CTACGGGCTT GGGATCTCAG CAAAATTTGC AAAATTCGCA TAATTCTGAG 
CAAAGAAATA ATGTGCAAAA TATGCATCAA AATCAATATA ATTCAAGTCA AAATCCGCCA 
ATACCTATCC GACAAATCGG AGCAGCATCA TCACACCAAC ATGATCAAGG ATCTCAGGGG 
CCTGGGGGAA AACCACAAGC CTATCACCTG GTGCAACAGG GATCACAGCA ACAGCAGCAG 
CAGCAGCAGC AGGCGACGTT ACAGCGAAGA AATGCGGCGG CGGCGGCAGG GTCGAATGTG 
CAGTTTATTC AGCAGCAGCA GCAGCAGCAG CAATCGGGTA AAAATTGCGG TCAAGGACAA 
AGCTTCGTTG TCATGGGCTC GCAGAGCTCA TCAAATGATG GACAAGGTGG AGCATCGACC 
GTCGGAGGAG GAGGAGGAGG ATCACAACAG CCTCACCAGC AGCAGCAGCA GCAGCCACAA 
CAAAGAATAC AGTACATTCC ACAAGTTACC GGTAGCGGAA ATAACGGTGG AGGTGGTGGA 
AGAGGAGGCT ACGGTAGTAC ACTGGTCATG CCAAGAGGAG GACGTGTTGT CAGGCCAGCG 
GGAACTCTAC CAGGCGGTGG ACGTCTGTAT GTCGATCATA ACCGTCATCC ATATCCAATG 
TCGTCAAATG TTGTGCCAGT ACGTGTTCTA CCAGCCACGC AACAAGGACA ACAACGAATG 
ATGACAGGAC AACGTCGTCC GGCTCCAGCG CCCGGTACTG TCGCCGCAAT GGTGTTGCCG 
AATCGAGGAG CTGGTGGAAT TCCGCAAATG CGCAGTTTGC AACGAGGCAG TTACACCGGC 
GGTGGTGGTC AGCAACGAAT CAACGTGATG GTTCAACCAC AACAAATGCG CAGCAACAAT 
GGCGGTGGAG TCGGTGGCCA AGGAGGCCTC CAGGGTGGTC CAGGAGGTCC GCAAGGAATT 
CGTCGGCCAC TCGTCGGACG GCCACTACAA CGAGGAGTCG ATAATCAGGC GCCGACGGTT 
GCTCAGGTCG TTGTTGCTCC GCCGCAAGGA ATGCAGCAGG CATCACAAGG ACCACCCGTA 
CTTCATATGC AGAGAGCGGT TTCCATGCAA ATGCCGACGA GTCATCATCA TCAAGGCCAA 
CAGCAGGCTC CTCCGCAGAG CTCACAGCAG GCTTCGCAAC AGGCTCCCAC ATCGGATTCT 
GGGACGAGTG CTCCGCCACG ACAAGCACCA CCACCACAAA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255182.1
CDS	1..2625
Translation	MPIWLPPSPPDSDADFDLRMEDDCLDLMYEIEQMNEARLPQVCHEMRRPLAEKQQKQNTLNAFNDILSAKEKESVYDAVNKCLQMPQSEAITAESAASPAYTEHSSFSMDDTSQDAKIEPSLTENQQPTTTATTTTTVPQQQQQQQQQKSSKKKRNDNRTAQNRTAENGVKRATTPPPSWREEPDYDGAEWNIVEDYALLQAVQVEFANAHLVEKSANEGMVLNWEFVSNAVNKQTRFFRSARQCSIRYQMFVRPKELGQLVASDPISKKTMKVDLSHTELSHLRKGRMTTESQYAHDYGILTDKKHVNRFKSVRVAATRRPVQFWRGPKALESRNLQSLNGGMPPRHESRLAEFDVKTNIRLDAEDIVTMSDESIVAYEASKKKLLASRQTKPSPRQDVRFHTLVLRPYTVPVTTEYSAAPSRREMRIAVPPLQPSALSTISSVAAAATSGPLPSIQHLQSSSTGLGSQQNLQNSHNSEQRNNVQNMHQNQYNSSQNPPIPIRQIGAASSHQHDQGSQGPGGKPQAYHLVQQGSQQQQQQQQQATLQRRNAAAAAGSNVQFIQQQQQQQQSGKNCGQGQSFVVMGSQSSSNDGQGGASTVGGGGGGSQQPHQQQQQQPQQRIQYIPQVTGSGNNGGGGGRGGYGSTLVMPRGGRVVRPAGTLPGGGRLYVDHNRHPYPMSSNVVPVRVLPATQQGQQRMMTGQRRPAPAPGTVAAMVLPNRGAGGIPQMRSLQRGSYTGGGGQQRINVMVQPQQMRSNNGGGVGGQGGLQGGPGGPQGIRRPLVGRPLQRGVDNQAPTVAQVVVAPPQGMQQASQGPPVLHMQRAVSMQMPTSHHHQGQQQAPPQSSQQASQQAPTSDSGTSAPPRQAPPPQN

Target ORF information:

RefSeq Version	NM_001268253.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268253.1

ORF Insert Sequence:

ATGCCAATCT GGCTTCCACC ATCACCACCA GATTCGGATG CGGATTTCGA CTTGAGAATG 
GAAGATGATT GTCTCGATCT GATGTATGAA ATTGAACAAA TGAACGAGGC TCGCCTACCA 
CAAGTTTGTC ATGAAATGAG ACGTCCGTTG GCTGAAAAAC AGCAGAAACA GAACACGTTG 
AATGCGTTTA ATGACATTCT ATCGGCAAAA GAAAAGGAAT CGGTGTACGA TGCGGTCAAC 
AAGTGCCTTC AAATGCCACA ATCCGAAGCG ATCACAGCAG AATCTGCAGC GTCTCCAGCA 
TACACGGAAC ACTCATCATT CTCGATGGAT GATACAAGCC AGGATGCGAA GATTGAGCCA 
AGTTTGACTG AAAATCAACA ACCCACCACC ACCGCCACTA CTACTACTAC AGTACCCCAA 
CAACAACAAC AACAGCAGCA GCAAAAATCG TCGAAAAAGA AGAGAAATGA TAATCGAACG 
GCTCAAAATC GAACAGCTGA AAATGGTGTG AAACGAGCGA CAACTCCACC ACCATCATGG 
CGTGAAGAGC CAGATTATGA TGGAGCCGAA TGGAATATAG TTGAAGATTA TGCACTACTT 
CAAGCAGTTC AAGTCGAATT TGCAAATGCT CATTTAGTCG AAAAATCGGC GAATGAGGGA 
ATGGTGTTGA ACTGGGAATT CGTGTCGAAT GCCGTTAATA AGCAGACAAG ATTTTTCCGC 
TCGGCCCGTC AATGCTCAAT TCGATATCAA ATGTTTGTTC GGCCAAAAGA GCTCGGACAG 
TTGGTGGCTT CTGATCCGAT TTCCAAGAAA ACGATGAAAG TCGACCTATC GCATACTGAA 
TTATCTCATT TGAGAAAAGG ACGAATGACT ACGGAGAGCC AATATGCTCA TGATTATGGA 
ATATTGACTG ATAAGAAACA TGTGAATAGA TTTAAAAGTG TTCGAGTGGC GGCAACACGG 
AGACCTGTTC AGTTTTGGAG AGGCCCTAAA GCTCTAGAAT CTCGAAATCT GCAATCTCTA 
AACGGCGGAA TGCCACCACG ACACGAGTCG AGACTCGCCG AATTCGACGT AAAAACCAAT 
ATTCGCCTGG ACGCCGAGGA CATTGTCACA ATGTCCGACG AGTCGATTGT CGCCTATGAA 
GCGAGCAAGA AGAAGCTACT GGCCAGTCGT CAAACAAAAC CCTCACCACG TCAAGATGTC 
CGATTCCATA CGCTGGTTCT TCGGCCGTAT ACCGTACCTG TGACAACTGA GTACTCGGCT 
GCACCTTCTC GTCGTGAAAT GCGCATCGCT GTTCCACCGC TTCAGCCTTC GGCTTTATCT 
ACGATTTCCT CAGTTGCTGC TGCTGCCACG TCTGGGCCAC TACCATCAAT TCAGCATTTG 
CAGTCGTCGT CTACGGGCTT GGGATCTCAG CAAAATTTGC AAAATTCGCA TAATTCTGAG 
CAAAGAAATA ATGTGCAAAA TATGCATCAA AATCAATATA ATTCAAGTCA AAATCCGCCA 
ATACCTATCC GACAAATCGG AGCAGCATCA TCACACCAAC ATGATCAAGG ATCTCAGGGG 
CCTGGGGGAA AACCACAAGC CTATCACCTG GTGCAACAGG GATCACAGCA ACAGCAGCAG 
CAGCAGCAGC AGGCGACGTT ACAGCGAAGA AATGCGGCGG CGGCGGCAGG GTCGAATGTG 
CAGTTTATTC AGCAGCAGCA GCAGCAGCAG CAATCGGGTA AAAATTGCGG TCAAGGACAA 
AGCTTCGTTG TCATGGGCTC GCAGAGCTCA TCAAATGATG GACAAGGTGG AGCATCGACC 
GTCGGAGGAG GAGGAGGAGG ATCACAACAG CCTCACCAGC AGCAGCAGCA GCAGCCACAA 
CAAAGAATAC AGTACATTCC ACAAGTTACC GGTAGCGGAA ATAACGGTGG AGGTGGTGGA 
AGAGGAGGCT ACGGTAGTAC ACTGGTCATG CCAAGAGGAG GACGTGTTGT CAGGCCAGCG 
GGAACTCTAC CAGGCGGTGG ACGTCTGTAT GTCGATCATA ACCGTCATCC ATATCCAATG 
TCGTCAAATG TTGTGCCAGT ACGTGTTCTA CCAGCCACGC AACAAGGACA ACAACGAATG 
ATGACAGGAC AACGTCGTCC GGCTCCAGCG CCCGGTACTG TCGCCGCAAT GGTGTTGCCG 
AATCGAGGAG CTGGTGGAAT TCCGCAAATG CGCAGTTTGC AACGAGGCAG TTACACCGGC 
GGTGGTGGTC AGCAACGAAT CAACGTGATG GTTCAACCAC AACAAATGCG CAGCAACAAT 
GGCGGTGGAG TCGGTGGCCA AGGAGGCCTC CAGGGTGGTC CAGGAGGTCC GCAAGGAATT 
CGTCGGCCAC TCGTCGGACG GCCACTACAA CGAGGAGTCG ATAATCAGGC GCCGACGGTT 
GCTCAGGTCG TTGTTGCTCC GCCGCAAGGA ATGCAGCAGG CATCACAAGG ACCACCCGTA 
CTTCATATGC AGAGAGCGGT TTCCATGCAA ATGCCGACGA GTCATCATCA TCAAGGCCAA 
CAGCAGGCTC CTCCGCAGAG CTCACAGCAG GCTTCGCAAC AGGCTCCCAC ATCGGATTCT 
GGGACGAGTG CTCCGCCACG ACAAGCACCA CCACCACAAA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssl-1 ( NM_001268252.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255181.1 Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.

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CloneID	OCa340604
Clone ID Related Accession (Same CDS sequence)	NM_001268252.1 , NM_001268252.2
Accession Version	NM_001268252.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Helicase ssl-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). COMPLETENESS: incomplete on both ends.

ATGGCAGATC TCACAAAAGA ACCACCACCA ACACCTGCAC CTCCTCCTCC AATCCAGAAG 
ACAATGCAAC CGATTGAGGT GAAAATTGAG GATTCAGAGG GCTCAAATAC GGCTCAACCG 
AGTGTTCTGC CCAGTTGTGG AGGAGGAGAG ACGAATGTGG AAAGAGCCGC CAAAAGAGAA 
GCGCATGTAT TGGCTCGAAT CGCCGAGCTC CGTAAGAACG GCTTATGGTC GAACAGTCGT 
CTGCCAAAGT GCGTCGAACC TGAACGTAAT AAAACGCATT GGGATTATCT ACTGGAAGAG 
GTCAAATGGA TGGCAGTTGA TTTCCGAACC GAGACGAATA CGAAGCGAAA AATCGCCAAA 
GTTATAGCTC ACGCCATTGC GAAACAGCAC CGCGACAAGC AGATCGAGAT TGAGAGAGCC 
GCCGAACGGG AGATCAAGGA GAAGCGAAAA ATGTGTGCAG GAATCGCGAA GATGGTACGG 
GATTTCTGGT CGTCTACGGA TAAAGTTGTG GATATTCGAG CGAAGGAAGT TCTGGAGTCG 
AGGCTCAGGA AGGCGAGAAA TAAGCATTTG ATGTTTGTAA TTGGACAAGT CGATGAAATG 
AGCAATATTG TGCAAGAAGG ACTTGTTTCA TCGTCGAAAT CCCCATCAAT TGCATCGGAT 
CGAGATGATA AAGATGAAGA ATTCAAAGCA CCTGGCTCTG ATTCAGAATC TGACGATGAG 
CAGACAATTG CAAACGCGGA AAAGTCACAG AAAAAGGAAG ATGTTCGACA GGAAGTTGAT 
GCTCTTCAAA ACGAGGCAAC TGTGGATATG GATGACTTTT TGTACACTTT ACCGCCGGAA 
TATCTGAAGG CTTATGGTCT GACGCAGGAG GATTTGGAGG AGATGAAGCG CGAGAAATTG 
GAGGAGCAGA AGGCTCGGAA GGAAGCTTGT GGTGATAATG AGGAGAAAAT GGAGATTGAT 
GAAAGCCCAT CATCAGATGC TCAAAAGCCT TCCACCTCAA GCTCAGATCT CACCGCCGAG 
CAGCTTCAAG ATCCAACAGC TGAAGACGGC AACGGTGATG GTCATGGTGT ACTTGAAAAC 
GTGGATTACG TGAAGCTCAA CAGTCAGGAT AGTGATGAAC GACAACAAGA GTTGGCGAAT 
ATCGCAGAAG AAGCGCTGAA ATTCCAGCCA AAAGGATATA CACTTGAGAC GACACAAGTC 
AAGACGCCCG TACCATTCCT GATTCGAGGA CAACTGAGAG AATATCAAAT GGTTGGATTG 
GATTGGATGG TTACACTTTA TGAGAAGAAT TTGAATGGAA TTCTTGCCGA CGAGATGGGC 
CTGGGAAAGA CGATTCAAAC GATTTCCCTG CTGGCTCATA TGGCTTGTAG TGAATCGATT 
TGGGGACCAC ACTTGATTGT TGTGCCGACG TCTGTCATTC TGAATTGGGA GATGGAGTTC 
AAGAAATGGT GTCCGGCTCT GAAGATTTTG ACGTATTTTG GTACGGCGAA GGAGCGTGCC 
GAGAAGCGGA AGGGATGGAT GAAGCCGAAT TGTTTCCATG TGTGCATCAC ATCATACAAG 
ACGGTTACTC AAGATATTAG AGCTTTTAAG CAGAGGGCCT GGCAGTACCT AATTCTCGAT 
GAAGCTCAAA ATATCAAAAA CTGGAAGTCC CAACGTTGGC AGGCTCTTCT GAATGTCCGT 
GCTCGACGTC GCCTTCTCCT GACCGGAACT CCACTTCAGA ACTCTCTAAT GGAACTGTGG 
TCGTTGATGC ATTTTTTGAT GCCAACAATA TTCTCAAGTC ATGATGATTT CAAGGATTGG 
TTCTCGAATC CGTTGACAGG GATGATGGAA GGAAATATGG AATTCAATGC TCCACTAATC 
GGACGACTTC ACAAAGTGCT CCGTCCGTTT ATTCTGCGGC GGCTCAAGAA GGAAGTTGAG 
AAGCAGCTGC CAGAGAAGAC TGAGCATATT GTGAATTGTT CGTTGTCAAA GCGGCAGAGA 
TACCTGTACG ATGACTTTAT GAGTCGTAGA TCAACAAAGG AGAATCTAAA GTCTGGAAAT 
ATGATGTCGG TGCTCAACAT TGTGATGCAA CTCCGAAAAT GTTGTAATCA TCCGAATCTC 
TTCGAGCCGC GGCCAGTTGT TGCTCCGTTC GTCGTTGAGA AGCTTCAGCT CGATGTTCCG 
GCTCGTCTCT TTGAAATTTC GCAGCAAGAT CCCTCCTCCT CCTCAGCTAG TCAAATTCCG 
GAAATTTTCA ATTTATCCAA AATCGGCTAT CAATCTTCCG TTCGATCTGC AAAACCACTC 
ATCGAAGAGC TTGAAGCAAT GAGCACTTAT CCGGAGCCAC GAGCACCAGA AGTTGGCGGA 
TTTCGGTTCA ATCGGACGGC TTTTGTTGCA AAGAATCCGC ATACGGAAGA GTCGGAGGAC 
GAAGGTGTTA TGAGAAGTCG TGTTCTGCCA AAACCAATTA ATGGAACAGC TCAACCACTT 
CAAAATGGAA ATTCAATACC ACAAAATGCT CCAAATCGTC CACAAACTTC ATGCATTCGT 
TCAAAAACCG TCGTAAATAC AGTTCCACTG ACCATCTCCA CCGATCGAAG TGGTTTTCAT 
TTTAATATGG CCAATGTTGG AAGAGGTGTT GTTCGTTTGG ATGATTCAGC ACGTATGAGC 
CCACCGCTCA AACGTCAGAA GCTCACCGGA ACTGCAACGA ATTGGAGTGA TTATGTTCCG 
CGACACGTTG TTGAAAAGAT GGAAGAATCG AGAAAAAACC AGCTGGAAAT TGTTCGAAGG 
CGATTTGAGA TGATTCGTGC TCCGATTATT CCACTGGAAA TGGTTGCGCT GGTTCGAGAG 
GAAATTATTG CAGAATTTCC ACGTTTGGCT GTGGAAGAGG ACGAGGTTGT GCAGGAGAGG 
CTTTTGGAGT ATTGCGAGTT GTTGGTGCAA AGATTCGGAA TGTACGTCGA ACCAGTGCTG 
ACCGATGCTT GGCAGTGTCG TCCATCATCG TCTGGTCTTC CATCATATAT TCGCAACAAT 
TTATCAAATA TCGAGCTGAA TTCTCGTTCT CTTCTCCTCA ACACCTCCAC TAATTTCGAT 
ACCCGAATGT CGATCTCACG TGCTCTTCAA TTCCCAGAAC TCCGTCTGAT CGAGTACGAT 
TGTGGAAAGC TTCAGACGTT GGCTGTTCTG CTTCGTCAGT TGTACCTGTA CAAGCACAGA 
TGTCTGATCT TCACGCAAAT GTCAAAGATG CTCGACGTTC TGCAGACCTT CCTTTCTCAT 
CACGGTTATC AGTATTTCCG CCTCGACGGT ACCACTGGTG TCGAACAAAG ACAGGCGATG 
ATGGAGCGGT TCAACGCGGA TCCCAAGGTG TTTTGCTTCA TTCTGTCGAC GAGATCCGGT 
GGTGTTGGAG TCAATCTAAC CGGTGCTGAC ACTGTGATCT TCTACGATTC GGATTGGAAT 
CCGACGATGG ATGCTCAGGC TCAGGATAGA TGTCATCGTA TCGGACAGAC GAGGAATGTC 
TCGATTTATC GATTGATTTC CGAGCGAACA ATTGAGGAGA ATATTCTGAG AAAGGCAACA 
CAGAAGCGGC GACTTGGAGA GTTGGCAATT GACGAGGCTG GCTTCACACC CGAGTTCTTC 
AAACAATCTG ACAGTATTCG GGATCTTTTT GATGGAGAGA ATGTGGAAGT GACTGCTGTG 
GCAGATGTTG CGACGACGAT GAGCGAGAAA GAAATGGAGG TTGCGATGGC AAAGTGTGAA 
GATGAAGCTG ATGTGAATGC GGCGAAGATT GCGGTGGCCG AGGCGAACGT TGATAATGCG 
GAGTTTGATG AGAAATCATT GCCGCCGATG AGCAATTTGC AAGGAGATGA GGAGGCTGAT 
GAGAAGTATA TGGAGTTGAT ACAACAGCTC AAACCAATCG AACGATATGC CATTAACTTT 
CTTGAGACAC AGTACAAGCC AGAATTTGAG GAAGAATGCA AAGAGGCAGA GGCTCTTATC 
GACCAAAAAC GCGAAGAATG GGACAAAAAT CTCAACGATA CCGCCGTCAT TGACCTCGAC 
GATTCGGATA GTCTGCTGCT CAACGATCCT TCGACTTCTG CCGATTTTTA TCAGAGCTCA 
AGTCTTTTAG ACGAGATAAA ATTCTACGAC GAGCTGGACG ATATCATGCC AATCTGGCTT 
CCACCATCAC CACCAGATTC GGATGCGGAT TTCGACTTGA GAATGGAAGA TGATTGTCTC 
GATCTGATGT ATGAAATTGA ACAAATGAAC GAGGCTCGCC TACCACAAGT TTGTCATGAA 
ATGAGACGTC CGTTGGCTGA AAAACAGCAG AAACAGAACA CGTTGAATGC GTTTAATGAC 
ATTCTATCGG CAAAAGAAAA GGAATCGGTG TACGATGCGG TCAACAAGTG CCTTCAAATG 
CCACAATCCG AAGCGATCAC AGCAGAATCT GCAGCGTCTC CAGCATACAC GGAACACTCA 
TCATTCTCGA TGGATGATAC AAGCCAGGAT GCGAAGATTG AGCCAAGTTT GACTGAAAAT 
CAACAACCCA CCACCACCGC CACTACTACT ACTACAGTAC CCCAACAACA ACAACAACAG 
CAGCAGCAAA AATCGTCGAA AAAGAAGAGA AATGATAATC GAACGGCTCA AAATCGAACA 
GCTGAAAATG GTGTGAAACG AGCGACAACT CCACCACCAT CATGGCGTGA AGAGCCAGAT 
TATGATGGAG CCGAATGGAA TATAGTTGAA GATTATGCAC TACTTCAAGC AGTTCAAGTC 
GAATTTGCAA ATGCTCATTT AGTCGAAAAA TCGGCGAATG AGGGAATGGT GTTGAACTGG 
GAATTCGTGT CGAATGCCGT TAATAAGCAG ACAAGATTTT TCCGCTCGGC CCGTCAATGC 
TCAATTCGAT ATCAAATGTT TGTTCGGCCA AAAGAGCTCG GACAGTTGGT GGCTTCTGAT 
CCGATTTCCA AGAAAACGAT GAAAGTCGAC CTATCGCATA CTGAATTATC TCATTTGAGA 
AAAGGACGAA TGACTACGGA GAGCCAATAT GCTCATGATT ATGGAATATT GACTGATAAG 
AAACATGTGA ATAGATTTAA AAGTGTTCGA GTGGCGGCAA CACGGAGACC TGTTCAGTTT 
TGGAGAGGCC CTAAAGCTCT AGAATCTCGA AATCTGCAAT CTCTAAACGG CGGAATGCCA 
CCACGACACG AGTCGAGACT CGCCGAATTC GACGTAAAAA CCAATATTCG CCTGGACGCC 
GAGGACATTG TCACAATGTC CGACGAGTCG ATTGTCGCCT ATGAAGCGAG CAAGAAGAAG 
CTACTGGCCA GTCGTCAAAC AAAACCCTCA CCACGTCAAG ATGTCCGATT CCATACGCTG 
GTTCTTCGGC CGTATACCGT ACCTGTGACA ACTGAGTACT CGGCTGCACC TTCTCGTCGT 
GAAATGCGCA TCGCTGTTCC ACCGCTTCAG CCTTCGGCTT TATCTACGAT TTCCTCAGTT 
GCTGCTGCTG CCACGTCTGG GCCACTACCA TCAATTCAGC ATTTGCAGTC GTCGTCTACG 
GGCTTGGGAT CTCAGCAAAA TTTGCAAAAT TCGCATAATT CTGAGCAAAG AAATAATGTG 
CAAAATATGC ATCAAAATCA ATATAATTCA AGTCAAAATC CGCCAATACC TATCCGACAA 
ATCGGAGCAG CATCATCACA CCAACATGAT CAAGGATCTC AGGGGCCTGG GGGAAAACCA 
CAAGCCTATC ACCTGGTGCA ACAGGGATCA CAGCAACAGC AGCAGCAGCA GCAGCAGGCG 
ACGTTACAGC GAAGAAATGC GGCGGCGGCG GCAGGGTCGA ATGTGCAGTT TATTCAGCAG 
CAGCAGCAGC AGCAGCAATC GGGTAAAAAT TGCGGTCAAG GACAAAGCTT CGTTGTCATG 
GGCTCGCAGA GCTCATCAAA TGATGGACAA GGTGGAGCAT CGACCGTCGG AGGAGGAGGA 
GGAGGATCAC AACAGCCTCA CCAGCAGCAG CAGCAGCAGC CACAACAAAG AATACAGTAC 
ATTCCACAAG TTACCGGTAG CGGAAATAAC GGTGGAGGTG GTGGAAGAGG AGGCTACGGT 
AGTACACTGG TCATGCCAAG AGGAGGACGT GTTGTCAGGC CAGCGGGAAC TCTACCAGGC 
GGTGGACGTC TGTATGTCGA TCATAACCGT CATCCATATC CAATGTCGTC AAATGTTGTG 
CCAGTACGTG TTCTACCAGC CACGCAACAA GGACAACAAC GAATGATGAC AGGACAACGT 
CGTCCGGCTC CAGCGCCCGG TACTGTCGCC GCAATGGTGT TGCCGAATCG AGGAGCTGGT 
GGAATTCCGC AAATGCGCAG TTTGCAACGA GGCAGTTACA CCGGCGGTGG TGGTCAGCAA 
CGAATCAACG TGATGGTTCA ACCACAACAA ATGCGCAGCA ACAATGGCGG TGGAGTCGGT 
GGCCAAGGAG GCCTCCAGGG TGGTCCAGGA GGTCCGCAAG GAATTCGTCG GCCACTCGTC 
GGACGGCCAC TACAACGAGG AGTCGATAAT CAGGCGCCGA CGGTTGCTCA GGTCGTTGTT 
GCTCCGCCGC AAGGAATGCA GCAGGCATCA CAAGGACCAC CCGTACTTCA TATGCAGAGA 
GCGGTTTCCA TGCAAATGCC GACGAGTCAT CATCATCAAG GCCAACAGCA GGCTCCTCCG 
CAGAGCTCAC AGCAGGCTTC GCAACAGGCT CCCACATCGG ATTCTGGGAC GAGTGCTCCG 
CCACGACAAG CACCACCACC ACAAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255181.1
CDS	1..6750
Translation	MADLTKEPPPTPAPPPPIQKTMQPIEVKIEDSEGSNTAQPSVLPSCGGGETNVERAAKREAHVLARIAELRKNGLWSNSRLPKCVEPERNKTHWDYLLEEVKWMAVDFRTETNTKRKIAKVIAHAIAKQHRDKQIEIERAAEREIKEKRKMCAGIAKMVRDFWSSTDKVVDIRAKEVLESRLRKARNKHLMFVIGQVDEMSNIVQEGLVSSSKSPSIASDRDDKDEEFKAPGSDSESDDEQTIANAEKSQKKEDVRQEVDALQNEATVDMDDFLYTLPPEYLKAYGLTQEDLEEMKREKLEEQKARKEACGDNEEKMEIDESPSSDAQKPSTSSSDLTAEQLQDPTAEDGNGDGHGVLENVDYVKLNSQDSDERQQELANIAEEALKFQPKGYTLETTQVKTPVPFLIRGQLREYQMVGLDWMVTLYEKNLNGILADEMGLGKTIQTISLLAHMACSESIWGPHLIVVPTSVILNWEMEFKKWCPALKILTYFGTAKERAEKRKGWMKPNCFHVCITSYKTVTQDIRAFKQRAWQYLILDEAQNIKNWKSQRWQALLNVRARRRLLLTGTPLQNSLMELWSLMHFLMPTIFSSHDDFKDWFSNPLTGMMEGNMEFNAPLIGRLHKVLRPFILRRLKKEVEKQLPEKTEHIVNCSLSKRQRYLYDDFMSRRSTKENLKSGNMMSVLNIVMQLRKCCNHPNLFEPRPVVAPFVVEKLQLDVPARLFEISQQDPSSSSASQIPEIFNLSKIGYQSSVRSAKPLIEELEAMSTYPEPRAPEVGGFRFNRTAFVAKNPHTEESEDEGVMRSRVLPKPINGTAQPLQNGNSIPQNAPNRPQTSCIRSKTVVNTVPLTISTDRSGFHFNMANVGRGVVRLDDSARMSPPLKRQKLTGTATNWSDYVPRHVVEKMEESRKNQLEIVRRRFEMIRAPIIPLEMVALVREEIIAEFPRLAVEEDEVVQERLLEYCELLVQRFGMYVEPVLTDAWQCRPSSSGLPSYIRNNLSNIELNSRSLLLNTSTNFDTRMSISRALQFPELRLIEYDCGKLQTLAVLLRQLYLYKHRCLIFTQMSKMLDVLQTFLSHHGYQYFRLDGTTGVEQRQAMMERFNADPKVFCFILSTRSGGVGVNLTGADTVIFYDSDWNPTMDAQAQDRCHRIGQTRNVSIYRLISERTIEENILRKATQKRRLGELAIDEAGFTPEFFKQSDSIRDLFDGENVEVTAVADVATTMSEKEMEVAMAKCEDEADVNAAKIAVAEANVDNAEFDEKSLPPMSNLQGDEEADEKYMELIQQLKPIERYAINFLETQYKPEFEEECKEAEALIDQKREEWDKNLNDTAVIDLDDSDSLLLNDPSTSADFYQSSSLLDEIKFYDELDDIMPIWLPPSPPDSDADFDLRMEDDCLDLMYEIEQMNEARLPQVCHEMRRPLAEKQQKQNTLNAFNDILSAKEKESVYDAVNKCLQMPQSEAITAESAASPAYTEHSSFSMDDTSQDAKIEPSLTENQQPTTTATTTTTVPQQQQQQQQQKSSKKKRNDNRTAQNRTAENGVKRATTPPPSWREEPDYDGAEWNIVEDYALLQAVQVEFANAHLVEKSANEGMVLNWEFVSNAVNKQTRFFRSARQCSIRYQMFVRPKELGQLVASDPISKKTMKVDLSHTELSHLRKGRMTTESQYAHDYGILTDKKHVNRFKSVRVAATRRPVQFWRGPKALESRNLQSLNGGMPPRHESRLAEFDVKTNIRLDAEDIVTMSDESIVAYEASKKKLLASRQTKPSPRQDVRFHTLVLRPYTVPVTTEYSAAPSRREMRIAVPPLQPSALSTISSVAAAATSGPLPSIQHLQSSSTGLGSQQNLQNSHNSEQRNNVQNMHQNQYNSSQNPPIPIRQIGAASSHQHDQGSQGPGGKPQAYHLVQQGSQQQQQQQQQATLQRRNAAAAAGSNVQFIQQQQQQQQSGKNCGQGQSFVVMGSQSSSNDGQGGASTVGGGGGGSQQPHQQQQQQPQQRIQYIPQVTGSGNNGGGGGRGGYGSTLVMPRGGRVVRPAGTLPGGGRLYVDHNRHPYPMSSNVVPVRVLPATQQGQQRMMTGQRRPAPAPGTVAAMVLPNRGAGGIPQMRSLQRGSYTGGGGQQRINVMVQPQQMRSNNGGGVGGQGGLQGGPGGPQGIRRPLVGRPLQRGVDNQAPTVAQVVVAPPQGMQQASQGPPVLHMQRAVSMQMPTSHHHQGQQQAPPQSSQQASQQAPTSDSGTSAPPRQAPPPQN

Target ORF information:

RefSeq Version	NM_001268252.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Helicase ssl-1 (ssl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268252.1

ORF Insert Sequence:

ATGGCAGATC TCACAAAAGA ACCACCACCA ACACCTGCAC CTCCTCCTCC AATCCAGAAG 
ACAATGCAAC CGATTGAGGT GAAAATTGAG GATTCAGAGG GCTCAAATAC GGCTCAACCG 
AGTGTTCTGC CCAGTTGTGG AGGAGGAGAG ACGAATGTGG AAAGAGCCGC CAAAAGAGAA 
GCGCATGTAT TGGCTCGAAT CGCCGAGCTC CGTAAGAACG GCTTATGGTC GAACAGTCGT 
CTGCCAAAGT GCGTCGAACC TGAACGTAAT AAAACGCATT GGGATTATCT ACTGGAAGAG 
GTCAAATGGA TGGCAGTTGA TTTCCGAACC GAGACGAATA CGAAGCGAAA AATCGCCAAA 
GTTATAGCTC ACGCCATTGC GAAACAGCAC CGCGACAAGC AGATCGAGAT TGAGAGAGCC 
GCCGAACGGG AGATCAAGGA GAAGCGAAAA ATGTGTGCAG GAATCGCGAA GATGGTACGG 
GATTTCTGGT CGTCTACGGA TAAAGTTGTG GATATTCGAG CGAAGGAAGT TCTGGAGTCG 
AGGCTCAGGA AGGCGAGAAA TAAGCATTTG ATGTTTGTAA TTGGACAAGT CGATGAAATG 
AGCAATATTG TGCAAGAAGG ACTTGTTTCA TCGTCGAAAT CCCCATCAAT TGCATCGGAT 
CGAGATGATA AAGATGAAGA ATTCAAAGCA CCTGGCTCTG ATTCAGAATC TGACGATGAG 
CAGACAATTG CAAACGCGGA AAAGTCACAG AAAAAGGAAG ATGTTCGACA GGAAGTTGAT 
GCTCTTCAAA ACGAGGCAAC TGTGGATATG GATGACTTTT TGTACACTTT ACCGCCGGAA 
TATCTGAAGG CTTATGGTCT GACGCAGGAG GATTTGGAGG AGATGAAGCG CGAGAAATTG 
GAGGAGCAGA AGGCTCGGAA GGAAGCTTGT GGTGATAATG AGGAGAAAAT GGAGATTGAT 
GAAAGCCCAT CATCAGATGC TCAAAAGCCT TCCACCTCAA GCTCAGATCT CACCGCCGAG 
CAGCTTCAAG ATCCAACAGC TGAAGACGGC AACGGTGATG GTCATGGTGT ACTTGAAAAC 
GTGGATTACG TGAAGCTCAA CAGTCAGGAT AGTGATGAAC GACAACAAGA GTTGGCGAAT 
ATCGCAGAAG AAGCGCTGAA ATTCCAGCCA AAAGGATATA CACTTGAGAC GACACAAGTC 
AAGACGCCCG TACCATTCCT GATTCGAGGA CAACTGAGAG AATATCAAAT GGTTGGATTG 
GATTGGATGG TTACACTTTA TGAGAAGAAT TTGAATGGAA TTCTTGCCGA CGAGATGGGC 
CTGGGAAAGA CGATTCAAAC GATTTCCCTG CTGGCTCATA TGGCTTGTAG TGAATCGATT 
TGGGGACCAC ACTTGATTGT TGTGCCGACG TCTGTCATTC TGAATTGGGA GATGGAGTTC 
AAGAAATGGT GTCCGGCTCT GAAGATTTTG ACGTATTTTG GTACGGCGAA GGAGCGTGCC 
GAGAAGCGGA AGGGATGGAT GAAGCCGAAT TGTTTCCATG TGTGCATCAC ATCATACAAG 
ACGGTTACTC AAGATATTAG AGCTTTTAAG CAGAGGGCCT GGCAGTACCT AATTCTCGAT 
GAAGCTCAAA ATATCAAAAA CTGGAAGTCC CAACGTTGGC AGGCTCTTCT GAATGTCCGT 
GCTCGACGTC GCCTTCTCCT GACCGGAACT CCACTTCAGA ACTCTCTAAT GGAACTGTGG 
TCGTTGATGC ATTTTTTGAT GCCAACAATA TTCTCAAGTC ATGATGATTT CAAGGATTGG 
TTCTCGAATC CGTTGACAGG GATGATGGAA GGAAATATGG AATTCAATGC TCCACTAATC 
GGACGACTTC ACAAAGTGCT CCGTCCGTTT ATTCTGCGGC GGCTCAAGAA GGAAGTTGAG 
AAGCAGCTGC CAGAGAAGAC TGAGCATATT GTGAATTGTT CGTTGTCAAA GCGGCAGAGA 
TACCTGTACG ATGACTTTAT GAGTCGTAGA TCAACAAAGG AGAATCTAAA GTCTGGAAAT 
ATGATGTCGG TGCTCAACAT TGTGATGCAA CTCCGAAAAT GTTGTAATCA TCCGAATCTC 
TTCGAGCCGC GGCCAGTTGT TGCTCCGTTC GTCGTTGAGA AGCTTCAGCT CGATGTTCCG 
GCTCGTCTCT TTGAAATTTC GCAGCAAGAT CCCTCCTCCT CCTCAGCTAG TCAAATTCCG 
GAAATTTTCA ATTTATCCAA AATCGGCTAT CAATCTTCCG TTCGATCTGC AAAACCACTC 
ATCGAAGAGC TTGAAGCAAT GAGCACTTAT CCGGAGCCAC GAGCACCAGA AGTTGGCGGA 
TTTCGGTTCA ATCGGACGGC TTTTGTTGCA AAGAATCCGC ATACGGAAGA GTCGGAGGAC 
GAAGGTGTTA TGAGAAGTCG TGTTCTGCCA AAACCAATTA ATGGAACAGC TCAACCACTT 
CAAAATGGAA ATTCAATACC ACAAAATGCT CCAAATCGTC CACAAACTTC ATGCATTCGT 
TCAAAAACCG TCGTAAATAC AGTTCCACTG ACCATCTCCA CCGATCGAAG TGGTTTTCAT 
TTTAATATGG CCAATGTTGG AAGAGGTGTT GTTCGTTTGG ATGATTCAGC ACGTATGAGC 
CCACCGCTCA AACGTCAGAA GCTCACCGGA ACTGCAACGA ATTGGAGTGA TTATGTTCCG 
CGACACGTTG TTGAAAAGAT GGAAGAATCG AGAAAAAACC AGCTGGAAAT TGTTCGAAGG 
CGATTTGAGA TGATTCGTGC TCCGATTATT CCACTGGAAA TGGTTGCGCT GGTTCGAGAG 
GAAATTATTG CAGAATTTCC ACGTTTGGCT GTGGAAGAGG ACGAGGTTGT GCAGGAGAGG 
CTTTTGGAGT ATTGCGAGTT GTTGGTGCAA AGATTCGGAA TGTACGTCGA ACCAGTGCTG 
ACCGATGCTT GGCAGTGTCG TCCATCATCG TCTGGTCTTC CATCATATAT TCGCAACAAT 
TTATCAAATA TCGAGCTGAA TTCTCGTTCT CTTCTCCTCA ACACCTCCAC TAATTTCGAT 
ACCCGAATGT CGATCTCACG TGCTCTTCAA TTCCCAGAAC TCCGTCTGAT CGAGTACGAT 
TGTGGAAAGC TTCAGACGTT GGCTGTTCTG CTTCGTCAGT TGTACCTGTA CAAGCACAGA 
TGTCTGATCT TCACGCAAAT GTCAAAGATG CTCGACGTTC TGCAGACCTT CCTTTCTCAT 
CACGGTTATC AGTATTTCCG CCTCGACGGT ACCACTGGTG TCGAACAAAG ACAGGCGATG 
ATGGAGCGGT TCAACGCGGA TCCCAAGGTG TTTTGCTTCA TTCTGTCGAC GAGATCCGGT 
GGTGTTGGAG TCAATCTAAC CGGTGCTGAC ACTGTGATCT TCTACGATTC GGATTGGAAT 
CCGACGATGG ATGCTCAGGC TCAGGATAGA TGTCATCGTA TCGGACAGAC GAGGAATGTC 
TCGATTTATC GATTGATTTC CGAGCGAACA ATTGAGGAGA ATATTCTGAG AAAGGCAACA 
CAGAAGCGGC GACTTGGAGA GTTGGCAATT GACGAGGCTG GCTTCACACC CGAGTTCTTC 
AAACAATCTG ACAGTATTCG GGATCTTTTT GATGGAGAGA ATGTGGAAGT GACTGCTGTG 
GCAGATGTTG CGACGACGAT GAGCGAGAAA GAAATGGAGG TTGCGATGGC AAAGTGTGAA 
GATGAAGCTG ATGTGAATGC GGCGAAGATT GCGGTGGCCG AGGCGAACGT TGATAATGCG 
GAGTTTGATG AGAAATCATT GCCGCCGATG AGCAATTTGC AAGGAGATGA GGAGGCTGAT 
GAGAAGTATA TGGAGTTGAT ACAACAGCTC AAACCAATCG AACGATATGC CATTAACTTT 
CTTGAGACAC AGTACAAGCC AGAATTTGAG GAAGAATGCA AAGAGGCAGA GGCTCTTATC 
GACCAAAAAC GCGAAGAATG GGACAAAAAT CTCAACGATA CCGCCGTCAT TGACCTCGAC 
GATTCGGATA GTCTGCTGCT CAACGATCCT TCGACTTCTG CCGATTTTTA TCAGAGCTCA 
AGTCTTTTAG ACGAGATAAA ATTCTACGAC GAGCTGGACG ATATCATGCC AATCTGGCTT 
CCACCATCAC CACCAGATTC GGATGCGGAT TTCGACTTGA GAATGGAAGA TGATTGTCTC 
GATCTGATGT ATGAAATTGA ACAAATGAAC GAGGCTCGCC TACCACAAGT TTGTCATGAA 
ATGAGACGTC CGTTGGCTGA AAAACAGCAG AAACAGAACA CGTTGAATGC GTTTAATGAC 
ATTCTATCGG CAAAAGAAAA GGAATCGGTG TACGATGCGG TCAACAAGTG CCTTCAAATG 
CCACAATCCG AAGCGATCAC AGCAGAATCT GCAGCGTCTC CAGCATACAC GGAACACTCA 
TCATTCTCGA TGGATGATAC AAGCCAGGAT GCGAAGATTG AGCCAAGTTT GACTGAAAAT 
CAACAACCCA CCACCACCGC CACTACTACT ACTACAGTAC CCCAACAACA ACAACAACAG 
CAGCAGCAAA AATCGTCGAA AAAGAAGAGA AATGATAATC GAACGGCTCA AAATCGAACA 
GCTGAAAATG GTGTGAAACG AGCGACAACT CCACCACCAT CATGGCGTGA AGAGCCAGAT 
TATGATGGAG CCGAATGGAA TATAGTTGAA GATTATGCAC TACTTCAAGC AGTTCAAGTC 
GAATTTGCAA ATGCTCATTT AGTCGAAAAA TCGGCGAATG AGGGAATGGT GTTGAACTGG 
GAATTCGTGT CGAATGCCGT TAATAAGCAG ACAAGATTTT TCCGCTCGGC CCGTCAATGC 
TCAATTCGAT ATCAAATGTT TGTTCGGCCA AAAGAGCTCG GACAGTTGGT GGCTTCTGAT 
CCGATTTCCA AGAAAACGAT GAAAGTCGAC CTATCGCATA CTGAATTATC TCATTTGAGA 
AAAGGACGAA TGACTACGGA GAGCCAATAT GCTCATGATT ATGGAATATT GACTGATAAG 
AAACATGTGA ATAGATTTAA AAGTGTTCGA GTGGCGGCAA CACGGAGACC TGTTCAGTTT 
TGGAGAGGCC CTAAAGCTCT AGAATCTCGA AATCTGCAAT CTCTAAACGG CGGAATGCCA 
CCACGACACG AGTCGAGACT CGCCGAATTC GACGTAAAAA CCAATATTCG CCTGGACGCC 
GAGGACATTG TCACAATGTC CGACGAGTCG ATTGTCGCCT ATGAAGCGAG CAAGAAGAAG 
CTACTGGCCA GTCGTCAAAC AAAACCCTCA CCACGTCAAG ATGTCCGATT CCATACGCTG 
GTTCTTCGGC CGTATACCGT ACCTGTGACA ACTGAGTACT CGGCTGCACC TTCTCGTCGT 
GAAATGCGCA TCGCTGTTCC ACCGCTTCAG CCTTCGGCTT TATCTACGAT TTCCTCAGTT 
GCTGCTGCTG CCACGTCTGG GCCACTACCA TCAATTCAGC ATTTGCAGTC GTCGTCTACG 
GGCTTGGGAT CTCAGCAAAA TTTGCAAAAT TCGCATAATT CTGAGCAAAG AAATAATGTG 
CAAAATATGC ATCAAAATCA ATATAATTCA AGTCAAAATC CGCCAATACC TATCCGACAA 
ATCGGAGCAG CATCATCACA CCAACATGAT CAAGGATCTC AGGGGCCTGG GGGAAAACCA 
CAAGCCTATC ACCTGGTGCA ACAGGGATCA CAGCAACAGC AGCAGCAGCA GCAGCAGGCG 
ACGTTACAGC GAAGAAATGC GGCGGCGGCG GCAGGGTCGA ATGTGCAGTT TATTCAGCAG 
CAGCAGCAGC AGCAGCAATC GGGTAAAAAT TGCGGTCAAG GACAAAGCTT CGTTGTCATG 
GGCTCGCAGA GCTCATCAAA TGATGGACAA GGTGGAGCAT CGACCGTCGG AGGAGGAGGA 
GGAGGATCAC AACAGCCTCA CCAGCAGCAG CAGCAGCAGC CACAACAAAG AATACAGTAC 
ATTCCACAAG TTACCGGTAG CGGAAATAAC GGTGGAGGTG GTGGAAGAGG AGGCTACGGT 
AGTACACTGG TCATGCCAAG AGGAGGACGT GTTGTCAGGC CAGCGGGAAC TCTACCAGGC 
GGTGGACGTC TGTATGTCGA TCATAACCGT CATCCATATC CAATGTCGTC AAATGTTGTG 
CCAGTACGTG TTCTACCAGC CACGCAACAA GGACAACAAC GAATGATGAC AGGACAACGT 
CGTCCGGCTC CAGCGCCCGG TACTGTCGCC GCAATGGTGT TGCCGAATCG AGGAGCTGGT 
GGAATTCCGC AAATGCGCAG TTTGCAACGA GGCAGTTACA CCGGCGGTGG TGGTCAGCAA 
CGAATCAACG TGATGGTTCA ACCACAACAA ATGCGCAGCA ACAATGGCGG TGGAGTCGGT 
GGCCAAGGAG GCCTCCAGGG TGGTCCAGGA GGTCCGCAAG GAATTCGTCG GCCACTCGTC 
GGACGGCCAC TACAACGAGG AGTCGATAAT CAGGCGCCGA CGGTTGCTCA GGTCGTTGTT 
GCTCCGCCGC AAGGAATGCA GCAGGCATCA CAAGGACCAC CCGTACTTCA TATGCAGAGA 
GCGGTTTCCA TGCAAATGCC GACGAGTCAT CATCATCAAG GCCAACAGCA GGCTCCTCCG 
CAGAGCTCAC AGCAGGCTTC GCAACAGGCT CCCACATCGG ATTCTGGGAC GAGTGCTCCG 
CCACGACAAG CACCACCACC ACAAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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ssl-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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