F25D7.5 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	F25D7.5
Entrez Gene ID	184923
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_060324.2	NP_492725.2	hypothetical protein
NM_060324.3	NP_492725.2	EGF-like domain-containing protein

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	F25D7.5	NP_492725.2
Caenorhabditis elegans (roundworm)	CELE_Y70G10A.2	NP_499266.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following F25D7.5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F25D7.5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa357403	NM_060324.3 Latest version!	Caenorhabditis elegans EGF-like domain-containing protein (F25D7.5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa357403	NM_060324.2	Caenorhabditis elegans Uncharacterized protein (F25D7.5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F25D7.5 ( NM_060324.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492725.2 Caenorhabditis elegans Uncharacterized protein (F25D7.5), partial mRNA.

Price & Availability↑

CloneID	OCa357403
Clone ID Related Accession (Same CDS sequence)	NM_060324.2 , NM_060324.3
Accession Version	NM_060324.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-09
Organism	Caenorhabditis elegans
Product	hypothetical protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 27, 2008 this sequence version replaced NM_060324.1. COMPLETENESS: incomplete on both ends.

ATGCGGAACA AATATATTTT CTCCATGTTT CCTATTATTT TGCTGGTTTT CTTTGGAAAA 
CTAGAAAGTA CTGATCGAAT GTCAACGAAT ACGATTCCAA TTGAACCTTC TACTCCTACA 
ACAACACCTT CTGATATTGT GCTCCCAGAA AACACTACTA TGTCCGATAT ATTAAAAAAT 
ACTAAAAGCG GGCAATGTGA AACATTGGAA GAAGTTCTGT GTGGATGTTC GACATGTTCA 
ACATCATGTC TTGGAACGTC AACGACAGTA GGATCTTCCA AGCGGAGTTC AATTTCCACT 
GTGACCAAAA ATGAAACGTC CACTAGATCA TCGTCATCTA CTTCGCAGAG CTTACCAACA 
AGCAAAAGTT TGGTTTCGAG CACCACATCC AACAAAGATC TTGAAACAAC TTCTGCTACG 
ATTACGGTCT CATCTAGCTC CATCACCAGC TCCACCAAAG AAATCTCAAG TAGAACAACT 
GGAAACTCAT CTGGAACTTC TGAAGTCACT TCAACTTTCT CTAAGGTGCC ACTGGCTACA 
CAATCCTCTC ACTCTACATC CTTGCCGTCA ACCCCATCTA CTTCAACTCA ACCAAGTTCA 
ACATCTGCCA AAGGCTCCAC AACATCCACT ATTGGTGATT TGAACACAAC TCGGACATCG 
ACGATTTCAA GCACAGAAAA ATCTAGCTCA ATGTCCCCAA CAATCAAAAG CTCATCTCCC 
TCCTCACCAC CTATGACACC CACAATTAAG ATTTCAACAC AGAAAGGATT ATCTTCTAGC 
ACAGTCAGTT TTCCGAGATG TTTCGTTTTC TATCAAAAAT CAACCTACAT TTCAACAAGA 
ACTGAGTCAA CCACCGTATC ACCGACATCA TCAAGTACTG CAATACAGGA TAATACAACA 
TTCTCCACAG CAAAATCCAC TGCAACTTCA AGCCAAACTC CAATTCATTC GACTTCAGCA 
ACCCTCGCAA GCAGTACCGT AAAATCTTCA TCTCCTGCTT CATCAGGATC TACTAGTTCC 
AGCTCCACCT CACAGCCTTC GACATCGTCA AAAAACACAG ATTCGTCAAC TGCACCTTGG 
ACTGAGCACG CAAAAGCTGA AGATATAATT TCAAGTACTA CTACTTCTAA AATAAGTTCA 
TCTTTGTCAT CTACCCAGTC TACAGCTTCA ACTTCAAGCA CTCCATATTC CACCTCGTCT 
TCAGAAACTC CAGATTCTTC TACCACCACG ACTGTGATAT CCACCACTAG AGATCCATCT 
TGTTCGGTGC AATGTCCTGA TGGATATGTT GCAGGTGAAA CAATGTGCTA CTACTTTGCC 
GAACTATCTG ACATCAAGTC CTATCAATCA GCTCTTTCTT ACTGCCAAAC AGTCAATCTT 
CGGAACCTGA TCTTTCTTGA GAATCTTCGA GATCTCAATG ATCTTCAAAC TCTTCGCTCA 
AAAAGTTCAT CAATTCAATC CACGTCGTTC TATTTTGCAA ACGGAGGAGG ATCTGTGCAA 
GAGCGGCTCA ACAAAAAAGC TCAAGTACTT GATATTACCC AATTAACAGC CATGACATCT 
CCAATTGCAA TGACAGTGGG GATATCTGAC ACTACGACGA ATGTTTCAGC GATATGTGTT 
CTTCCACAAT ACTGTAACAT ATCCAAATGC TACGTGGAAA CTGCTCTAAA CATCCTCGAA 
CTCAACTCAA TTCTCTCTTC ATCAAAAGAA ACAATTGATA CTACTTCCAC TGCAAGCCTA 
ACATGCTCCT ACACAAAATC AATTACAACT GTCTCATGTG GAACACGTGG AAATATAATG 
CCAAGTCCAA CAACTATCAG TTGTGCAGCA GAAGGTGTTG CTCAGAAGCT TGCAAATACA 
ACGTCTGATT TTGTCTCTAG TTGCGCTATG TGTTTTACTC GTGGAACTGA CTTCTGTGAG 
ACAACAGTTG ATGCAGATGG AAATTATGCC TATCAATGTC ATTGCAAGCC TAAATATTCT 
ATGAGCACCT GCTGGTACAC TCCTGATGCT TGCACTCCAA CTACATGTAA TGGTCATGGA 
AAGTGTTATG ATTACGTAGA AGATGTGAAA TGTGACTGTT ACTGGGGATA CGAGGGCGAA 
CATTGTGAAG TGAATAAGGA TCGAGTCACA AATACAACTG ACTCATTTTT CACTCGTTTT 
GGAAATTATA TTTTCGATTC TCCGACTATC AATTCTCTCG TGTCCTGTTC TTCGACAGTG 
CTCCTGATCG GTGTTAAATC TGTTGGAAAA ATTTACTTGT CGAATGGAGA AGATGATCCA 
CAAGAAACTC ATCAAACGGC TCGATCATTT TTATTGACTC TCGGCACATC CTGCGTACTC 
TTTTTCAACG ATCCGTATAT GTTCAAAATC TCACAAGCCA CGTGTCGGAT GTTCTTTATC 
GTTATACATT TTTGTTTCAT TGGAGCAATG ATCCAGTGGA TGCTAGAAGG ATATAATGCA 
AATCAAGTGG TTCGATGTGT TCATCTAAAT GAATGGGAAC GAGATTTTGA ATGTAGAAGA 
GCATGGGGTA TAATGGTTGC TCCACGAATG GTTGTACCAT TGCTTTTATT GGCAGTAGCA 
CTGGGTGTCC TTTTTCAATC AAACTGGAAT CAACTTCCTT CAACATGGAC ATGCGTTGGT 
GTGATTTGCA ATGAAACAAC TTCAGTTTGG TTATCAGCAA TATGGATTGT TCTTTGTATT 
GTTGTCTCTA CGGCAACATT TGCAGAATCC AGTGTTTTAA TCAAAAATCG CCGTCCCTTG 
TACAATCTGA AAATTCGACA ACGAATCGAA CATAAGGAGG GAGTCATTGA TGGTTGGGTT 
GCCGAAAAGT GCCGAAGAAA TACAGTTTTA TGTTTTATTG GAGTTTCTCT TCTGTCTGCA 
ACGTGGCTCC TGACCATTCT AGCCGCGGAT AAACGGAGCA AAGGAATTTA TGGATGGGTT 
CTCACACTCG TGGGAATCAT TTATGGAGTA TTCAGTTTTG TCCAAGGACT CTACACGGAT 
CCAAATTGTT GGTCCTCTAT TCTATGGCTC GCGATGAAAA AGTTTCCTGC TCGATTTGCT 
CCATCCTATG ATCCGATTTC CATGTGGACT CGTGAGGAAG TGAAAGAAAT TCAAAAGCTT 
CCAAAAGAAC AACAAGAAAT AATGAAACAA CAAATGCTTC CATTGAATAA AAGATTATTC 
CTACATCACA AATGGGATTT GATTCTCAAT CAGAAATTGG AAGCAGGACT GGATGTCTCT 
GCTGGTTTAC TGGAAATTCT GCGAACTGAG ATAAAGAAGC ATCATGATTT GGCTGGAACT 
CAATTTCAAA AAGAACAACT CCAAGAAACA TTTGCGGAAT TTGTGAAATC AGTCGCTGAA 
CGACCACCAA GAAATGATTT ATTGGGAACC AATGCAAAGA GTGAAGTTGT GACTCTATGT 
GCAGAAAAAG AGGATGGAAC TGCAAGAGTG ACCAAATTTA TGCTAGTCCC CGGAATCGAT 
GTTTTCAAAC CAGAAGAGTA CTATGATGAT GCTGATGATA TTGACAGAAA GAGATTTAAT 
AATGAAATTG ATAAGGAAAC CTACAAAATT GCAAGAGAAG AAGCAACGTC TCAAAACAAC 
TTTATGAATT CGGCCATCAA ATTCAAGTAC TACGGAAAAA TCAAAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492725.2
CDS	1..3651
Translation	MRNKYIFSMFPIILLVFFGKLESTDRMSTNTIPIEPSTPTTTPSDIVLPENTTMSDILKNTKSGQCETLEEVLCGCSTCSTSCLGTSTTVGSSKRSSISTVTKNETSTRSSSSTSQSLPTSKSLVSSTTSNKDLETTSATITVSSSSITSSTKEISSRTTGNSSGTSEVTSTFSKVPLATQSSHSTSLPSTPSTSTQPSSTSAKGSTTSTIGDLNTTRTSTISSTEKSSSMSPTIKSSSPSSPPMTPTIKISTQKGLSSSTVSFPRCFVFYQKSTYISTRTESTTVSPTSSSTAIQDNTTFSTAKSTATSSQTPIHSTSATLASSTVKSSSPASSGSTSSSSTSQPSTSSKNTDSSTAPWTEHAKAEDIISSTTTSKISSSLSSTQSTASTSSTPYSTSSSETPDSSTTTTVISTTRDPSCSVQCPDGYVAGETMCYYFAELSDIKSYQSALSYCQTVNLRNLIFLENLRDLNDLQTLRSKSSSIQSTSFYFANGGGSVQERLNKKAQVLDITQLTAMTSPIAMTVGISDTTTNVSAICVLPQYCNISKCYVETALNILELNSILSSSKETIDTTSTASLTCSYTKSITTVSCGTRGNIMPSPTTISCAAEGVAQKLANTTSDFVSSCAMCFTRGTDFCETTVDADGNYAYQCHCKPKYSMSTCWYTPDACTPTTCNGHGKCYDYVEDVKCDCYWGYEGEHCEVNKDRVTNTTDSFFTRFGNYIFDSPTINSLVSCSSTVLLIGVKSVGKIYLSNGEDDPQETHQTARSFLLTLGTSCVLFFNDPYMFKISQATCRMFFIVIHFCFIGAMIQWMLEGYNANQVVRCVHLNEWERDFECRRAWGIMVAPRMVVPLLLLAVALGVLFQSNWNQLPSTWTCVGVICNETTSVWLSAIWIVLCIVVSTATFAESSVLIKNRRPLYNLKIRQRIEHKEGVIDGWVAEKCRRNTVLCFIGVSLLSATWLLTILAADKRSKGIYGWVLTLVGIIYGVFSFVQGLYTDPNCWSSILWLAMKKFPARFAPSYDPISMWTREEVKEIQKLPKEQQEIMKQQMLPLNKRLFLHHKWDLILNQKLEAGLDVSAGLLEILRTEIKKHHDLAGTQFQKEQLQETFAEFVKSVAERPPRNDLLGTNAKSEVVTLCAEKEDGTARVTKFMLVPGIDVFKPEEYYDDADDIDRKRFNNEIDKETYKIAREEATSQNNFMNSAIKFKYYGKIKR

Target ORF information:

RefSeq Version	NM_060324.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (F25D7.5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_060324.2

ORF Insert Sequence:

ATGCGGAACA AATATATTTT CTCCATGTTT CCTATTATTT TGCTGGTTTT CTTTGGAAAA 
CTAGAAAGTA CTGATCGAAT GTCAACGAAT ACGATTCCAA TTGAACCTTC TACTCCTACA 
ACAACACCTT CTGATATTGT GCTCCCAGAA AACACTACTA TGTCCGATAT ATTAAAAAAT 
ACTAAAAGCG GGCAATGTGA AACATTGGAA GAAGTTCTGT GTGGATGTTC GACATGTTCA 
ACATCATGTC TTGGAACGTC AACGACAGTA GGATCTTCCA AGCGGAGTTC AATTTCCACT 
GTGACCAAAA ATGAAACGTC CACTAGATCA TCGTCATCTA CTTCGCAGAG CTTACCAACA 
AGCAAAAGTT TGGTTTCGAG CACCACATCC AACAAAGATC TTGAAACAAC TTCTGCTACG 
ATTACGGTCT CATCTAGCTC CATCACCAGC TCCACCAAAG AAATCTCAAG TAGAACAACT 
GGAAACTCAT CTGGAACTTC TGAAGTCACT TCAACTTTCT CTAAGGTGCC ACTGGCTACA 
CAATCCTCTC ACTCTACATC CTTGCCGTCA ACCCCATCTA CTTCAACTCA ACCAAGTTCA 
ACATCTGCCA AAGGCTCCAC AACATCCACT ATTGGTGATT TGAACACAAC TCGGACATCG 
ACGATTTCAA GCACAGAAAA ATCTAGCTCA ATGTCCCCAA CAATCAAAAG CTCATCTCCC 
TCCTCACCAC CTATGACACC CACAATTAAG ATTTCAACAC AGAAAGGATT ATCTTCTAGC 
ACAGTCAGTT TTCCGAGATG TTTCGTTTTC TATCAAAAAT CAACCTACAT TTCAACAAGA 
ACTGAGTCAA CCACCGTATC ACCGACATCA TCAAGTACTG CAATACAGGA TAATACAACA 
TTCTCCACAG CAAAATCCAC TGCAACTTCA AGCCAAACTC CAATTCATTC GACTTCAGCA 
ACCCTCGCAA GCAGTACCGT AAAATCTTCA TCTCCTGCTT CATCAGGATC TACTAGTTCC 
AGCTCCACCT CACAGCCTTC GACATCGTCA AAAAACACAG ATTCGTCAAC TGCACCTTGG 
ACTGAGCACG CAAAAGCTGA AGATATAATT TCAAGTACTA CTACTTCTAA AATAAGTTCA 
TCTTTGTCAT CTACCCAGTC TACAGCTTCA ACTTCAAGCA CTCCATATTC CACCTCGTCT 
TCAGAAACTC CAGATTCTTC TACCACCACG ACTGTGATAT CCACCACTAG AGATCCATCT 
TGTTCGGTGC AATGTCCTGA TGGATATGTT GCAGGTGAAA CAATGTGCTA CTACTTTGCC 
GAACTATCTG ACATCAAGTC CTATCAATCA GCTCTTTCTT ACTGCCAAAC AGTCAATCTT 
CGGAACCTGA TCTTTCTTGA GAATCTTCGA GATCTCAATG ATCTTCAAAC TCTTCGCTCA 
AAAAGTTCAT CAATTCAATC CACGTCGTTC TATTTTGCAA ACGGAGGAGG ATCTGTGCAA 
GAGCGGCTCA ACAAAAAAGC TCAAGTACTT GATATTACCC AATTAACAGC CATGACATCT 
CCAATTGCAA TGACAGTGGG GATATCTGAC ACTACGACGA ATGTTTCAGC GATATGTGTT 
CTTCCACAAT ACTGTAACAT ATCCAAATGC TACGTGGAAA CTGCTCTAAA CATCCTCGAA 
CTCAACTCAA TTCTCTCTTC ATCAAAAGAA ACAATTGATA CTACTTCCAC TGCAAGCCTA 
ACATGCTCCT ACACAAAATC AATTACAACT GTCTCATGTG GAACACGTGG AAATATAATG 
CCAAGTCCAA CAACTATCAG TTGTGCAGCA GAAGGTGTTG CTCAGAAGCT TGCAAATACA 
ACGTCTGATT TTGTCTCTAG TTGCGCTATG TGTTTTACTC GTGGAACTGA CTTCTGTGAG 
ACAACAGTTG ATGCAGATGG AAATTATGCC TATCAATGTC ATTGCAAGCC TAAATATTCT 
ATGAGCACCT GCTGGTACAC TCCTGATGCT TGCACTCCAA CTACATGTAA TGGTCATGGA 
AAGTGTTATG ATTACGTAGA AGATGTGAAA TGTGACTGTT ACTGGGGATA CGAGGGCGAA 
CATTGTGAAG TGAATAAGGA TCGAGTCACA AATACAACTG ACTCATTTTT CACTCGTTTT 
GGAAATTATA TTTTCGATTC TCCGACTATC AATTCTCTCG TGTCCTGTTC TTCGACAGTG 
CTCCTGATCG GTGTTAAATC TGTTGGAAAA ATTTACTTGT CGAATGGAGA AGATGATCCA 
CAAGAAACTC ATCAAACGGC TCGATCATTT TTATTGACTC TCGGCACATC CTGCGTACTC 
TTTTTCAACG ATCCGTATAT GTTCAAAATC TCACAAGCCA CGTGTCGGAT GTTCTTTATC 
GTTATACATT TTTGTTTCAT TGGAGCAATG ATCCAGTGGA TGCTAGAAGG ATATAATGCA 
AATCAAGTGG TTCGATGTGT TCATCTAAAT GAATGGGAAC GAGATTTTGA ATGTAGAAGA 
GCATGGGGTA TAATGGTTGC TCCACGAATG GTTGTACCAT TGCTTTTATT GGCAGTAGCA 
CTGGGTGTCC TTTTTCAATC AAACTGGAAT CAACTTCCTT CAACATGGAC ATGCGTTGGT 
GTGATTTGCA ATGAAACAAC TTCAGTTTGG TTATCAGCAA TATGGATTGT TCTTTGTATT 
GTTGTCTCTA CGGCAACATT TGCAGAATCC AGTGTTTTAA TCAAAAATCG CCGTCCCTTG 
TACAATCTGA AAATTCGACA ACGAATCGAA CATAAGGAGG GAGTCATTGA TGGTTGGGTT 
GCCGAAAAGT GCCGAAGAAA TACAGTTTTA TGTTTTATTG GAGTTTCTCT TCTGTCTGCA 
ACGTGGCTCC TGACCATTCT AGCCGCGGAT AAACGGAGCA AAGGAATTTA TGGATGGGTT 
CTCACACTCG TGGGAATCAT TTATGGAGTA TTCAGTTTTG TCCAAGGACT CTACACGGAT 
CCAAATTGTT GGTCCTCTAT TCTATGGCTC GCGATGAAAA AGTTTCCTGC TCGATTTGCT 
CCATCCTATG ATCCGATTTC CATGTGGACT CGTGAGGAAG TGAAAGAAAT TCAAAAGCTT 
CCAAAAGAAC AACAAGAAAT AATGAAACAA CAAATGCTTC CATTGAATAA AAGATTATTC 
CTACATCACA AATGGGATTT GATTCTCAAT CAGAAATTGG AAGCAGGACT GGATGTCTCT 
GCTGGTTTAC TGGAAATTCT GCGAACTGAG ATAAAGAAGC ATCATGATTT GGCTGGAACT 
CAATTTCAAA AAGAACAACT CCAAGAAACA TTTGCGGAAT TTGTGAAATC AGTCGCTGAA 
CGACCACCAA GAAATGATTT ATTGGGAACC AATGCAAAGA GTGAAGTTGT GACTCTATGT 
GCAGAAAAAG AGGATGGAAC TGCAAGAGTG ACCAAATTTA TGCTAGTCCC CGGAATCGAT 
GTTTTCAAAC CAGAAGAGTA CTATGATGAT GCTGATGATA TTGACAGAAA GAGATTTAAT 
AATGAAATTG ATAAGGAAAC CTACAAAATT GCAAGAGAAG AAGCAACGTC TCAAAACAAC 
TTTATGAATT CGGCCATCAA ATTCAAGTAC TACGGAAAAA TCAAAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F25D7.5 ( NM_060324.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492725.2 Caenorhabditis elegans EGF-like domain-containing protein (F25D7.5), mRNA.

Price & Availability↑

CloneID	OCa357403
Clone ID Related Accession (Same CDS sequence)	NM_060324.2 , NM_060324.3
Accession Version	NM_060324.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	EGF-like domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_060324.2.

ATGCGGAACA AATATATTTT CTCCATGTTT CCTATTATTT TGCTGGTTTT CTTTGGAAAA 
CTAGAAAGTA CTGATCGAAT GTCAACGAAT ACGATTCCAA TTGAACCTTC TACTCCTACA 
ACAACACCTT CTGATATTGT GCTCCCAGAA AACACTACTA TGTCCGATAT ATTAAAAAAT 
ACTAAAAGCG GGCAATGTGA AACATTGGAA GAAGTTCTGT GTGGATGTTC GACATGTTCA 
ACATCATGTC TTGGAACGTC AACGACAGTA GGATCTTCCA AGCGGAGTTC AATTTCCACT 
GTGACCAAAA ATGAAACGTC CACTAGATCA TCGTCATCTA CTTCGCAGAG CTTACCAACA 
AGCAAAAGTT TGGTTTCGAG CACCACATCC AACAAAGATC TTGAAACAAC TTCTGCTACG 
ATTACGGTCT CATCTAGCTC CATCACCAGC TCCACCAAAG AAATCTCAAG TAGAACAACT 
GGAAACTCAT CTGGAACTTC TGAAGTCACT TCAACTTTCT CTAAGGTGCC ACTGGCTACA 
CAATCCTCTC ACTCTACATC CTTGCCGTCA ACCCCATCTA CTTCAACTCA ACCAAGTTCA 
ACATCTGCCA AAGGCTCCAC AACATCCACT ATTGGTGATT TGAACACAAC TCGGACATCG 
ACGATTTCAA GCACAGAAAA ATCTAGCTCA ATGTCCCCAA CAATCAAAAG CTCATCTCCC 
TCCTCACCAC CTATGACACC CACAATTAAG ATTTCAACAC AGAAAGGATT ATCTTCTAGC 
ACAGTCAGTT TTCCGAGATG TTTCGTTTTC TATCAAAAAT CAACCTACAT TTCAACAAGA 
ACTGAGTCAA CCACCGTATC ACCGACATCA TCAAGTACTG CAATACAGGA TAATACAACA 
TTCTCCACAG CAAAATCCAC TGCAACTTCA AGCCAAACTC CAATTCATTC GACTTCAGCA 
ACCCTCGCAA GCAGTACCGT AAAATCTTCA TCTCCTGCTT CATCAGGATC TACTAGTTCC 
AGCTCCACCT CACAGCCTTC GACATCGTCA AAAAACACAG ATTCGTCAAC TGCACCTTGG 
ACTGAGCACG CAAAAGCTGA AGATATAATT TCAAGTACTA CTACTTCTAA AATAAGTTCA 
TCTTTGTCAT CTACCCAGTC TACAGCTTCA ACTTCAAGCA CTCCATATTC CACCTCGTCT 
TCAGAAACTC CAGATTCTTC TACCACCACG ACTGTGATAT CCACCACTAG AGATCCATCT 
TGTTCGGTGC AATGTCCTGA TGGATATGTT GCAGGTGAAA CAATGTGCTA CTACTTTGCC 
GAACTATCTG ACATCAAGTC CTATCAATCA GCTCTTTCTT ACTGCCAAAC AGTCAATCTT 
CGGAACCTGA TCTTTCTTGA GAATCTTCGA GATCTCAATG ATCTTCAAAC TCTTCGCTCA 
AAAAGTTCAT CAATTCAATC CACGTCGTTC TATTTTGCAA ACGGAGGAGG ATCTGTGCAA 
GAGCGGCTCA ACAAAAAAGC TCAAGTACTT GATATTACCC AATTAACAGC CATGACATCT 
CCAATTGCAA TGACAGTGGG GATATCTGAC ACTACGACGA ATGTTTCAGC GATATGTGTT 
CTTCCACAAT ACTGTAACAT ATCCAAATGC TACGTGGAAA CTGCTCTAAA CATCCTCGAA 
CTCAACTCAA TTCTCTCTTC ATCAAAAGAA ACAATTGATA CTACTTCCAC TGCAAGCCTA 
ACATGCTCCT ACACAAAATC AATTACAACT GTCTCATGTG GAACACGTGG AAATATAATG 
CCAAGTCCAA CAACTATCAG TTGTGCAGCA GAAGGTGTTG CTCAGAAGCT TGCAAATACA 
ACGTCTGATT TTGTCTCTAG TTGCGCTATG TGTTTTACTC GTGGAACTGA CTTCTGTGAG 
ACAACAGTTG ATGCAGATGG AAATTATGCC TATCAATGTC ATTGCAAGCC TAAATATTCT 
ATGAGCACCT GCTGGTACAC TCCTGATGCT TGCACTCCAA CTACATGTAA TGGTCATGGA 
AAGTGTTATG ATTACGTAGA AGATGTGAAA TGTGACTGTT ACTGGGGATA CGAGGGCGAA 
CATTGTGAAG TGAATAAGGA TCGAGTCACA AATACAACTG ACTCATTTTT CACTCGTTTT 
GGAAATTATA TTTTCGATTC TCCGACTATC AATTCTCTCG TGTCCTGTTC TTCGACAGTG 
CTCCTGATCG GTGTTAAATC TGTTGGAAAA ATTTACTTGT CGAATGGAGA AGATGATCCA 
CAAGAAACTC ATCAAACGGC TCGATCATTT TTATTGACTC TCGGCACATC CTGCGTACTC 
TTTTTCAACG ATCCGTATAT GTTCAAAATC TCACAAGCCA CGTGTCGGAT GTTCTTTATC 
GTTATACATT TTTGTTTCAT TGGAGCAATG ATCCAGTGGA TGCTAGAAGG ATATAATGCA 
AATCAAGTGG TTCGATGTGT TCATCTAAAT GAATGGGAAC GAGATTTTGA ATGTAGAAGA 
GCATGGGGTA TAATGGTTGC TCCACGAATG GTTGTACCAT TGCTTTTATT GGCAGTAGCA 
CTGGGTGTCC TTTTTCAATC AAACTGGAAT CAACTTCCTT CAACATGGAC ATGCGTTGGT 
GTGATTTGCA ATGAAACAAC TTCAGTTTGG TTATCAGCAA TATGGATTGT TCTTTGTATT 
GTTGTCTCTA CGGCAACATT TGCAGAATCC AGTGTTTTAA TCAAAAATCG CCGTCCCTTG 
TACAATCTGA AAATTCGACA ACGAATCGAA CATAAGGAGG GAGTCATTGA TGGTTGGGTT 
GCCGAAAAGT GCCGAAGAAA TACAGTTTTA TGTTTTATTG GAGTTTCTCT TCTGTCTGCA 
ACGTGGCTCC TGACCATTCT AGCCGCGGAT AAACGGAGCA AAGGAATTTA TGGATGGGTT 
CTCACACTCG TGGGAATCAT TTATGGAGTA TTCAGTTTTG TCCAAGGACT CTACACGGAT 
CCAAATTGTT GGTCCTCTAT TCTATGGCTC GCGATGAAAA AGTTTCCTGC TCGATTTGCT 
CCATCCTATG ATCCGATTTC CATGTGGACT CGTGAGGAAG TGAAAGAAAT TCAAAAGCTT 
CCAAAAGAAC AACAAGAAAT AATGAAACAA CAAATGCTTC CATTGAATAA AAGATTATTC 
CTACATCACA AATGGGATTT GATTCTCAAT CAGAAATTGG AAGCAGGACT GGATGTCTCT 
GCTGGTTTAC TGGAAATTCT GCGAACTGAG ATAAAGAAGC ATCATGATTT GGCTGGAACT 
CAATTTCAAA AAGAACAACT CCAAGAAACA TTTGCGGAAT TTGTGAAATC AGTCGCTGAA 
CGACCACCAA GAAATGATTT ATTGGGAACC AATGCAAAGA GTGAAGTTGT GACTCTATGT 
GCAGAAAAAG AGGATGGAAC TGCAAGAGTG ACCAAATTTA TGCTAGTCCC CGGAATCGAT 
GTTTTCAAAC CAGAAGAGTA CTATGATGAT GCTGATGATA TTGACAGAAA GAGATTTAAT 
AATGAAATTG ATAAGGAAAC CTACAAAATT GCAAGAGAAG AAGCAACGTC TCAAAACAAC 
TTTATGAATT CGGCCATCAA ATTCAAGTAC TACGGAAAAA TCAAAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492725.2
CDS	9..3659
Translation	MRNKYIFSMFPIILLVFFGKLESTDRMSTNTIPIEPSTPTTTPSDIVLPENTTMSDILKNTKSGQCETLEEVLCGCSTCSTSCLGTSTTVGSSKRSSISTVTKNETSTRSSSSTSQSLPTSKSLVSSTTSNKDLETTSATITVSSSSITSSTKEISSRTTGNSSGTSEVTSTFSKVPLATQSSHSTSLPSTPSTSTQPSSTSAKGSTTSTIGDLNTTRTSTISSTEKSSSMSPTIKSSSPSSPPMTPTIKISTQKGLSSSTVSFPRCFVFYQKSTYISTRTESTTVSPTSSSTAIQDNTTFSTAKSTATSSQTPIHSTSATLASSTVKSSSPASSGSTSSSSTSQPSTSSKNTDSSTAPWTEHAKAEDIISSTTTSKISSSLSSTQSTASTSSTPYSTSSSETPDSSTTTTVISTTRDPSCSVQCPDGYVAGETMCYYFAELSDIKSYQSALSYCQTVNLRNLIFLENLRDLNDLQTLRSKSSSIQSTSFYFANGGGSVQERLNKKAQVLDITQLTAMTSPIAMTVGISDTTTNVSAICVLPQYCNISKCYVETALNILELNSILSSSKETIDTTSTASLTCSYTKSITTVSCGTRGNIMPSPTTISCAAEGVAQKLANTTSDFVSSCAMCFTRGTDFCETTVDADGNYAYQCHCKPKYSMSTCWYTPDACTPTTCNGHGKCYDYVEDVKCDCYWGYEGEHCEVNKDRVTNTTDSFFTRFGNYIFDSPTINSLVSCSSTVLLIGVKSVGKIYLSNGEDDPQETHQTARSFLLTLGTSCVLFFNDPYMFKISQATCRMFFIVIHFCFIGAMIQWMLEGYNANQVVRCVHLNEWERDFECRRAWGIMVAPRMVVPLLLLAVALGVLFQSNWNQLPSTWTCVGVICNETTSVWLSAIWIVLCIVVSTATFAESSVLIKNRRPLYNLKIRQRIEHKEGVIDGWVAEKCRRNTVLCFIGVSLLSATWLLTILAADKRSKGIYGWVLTLVGIIYGVFSFVQGLYTDPNCWSSILWLAMKKFPARFAPSYDPISMWTREEVKEIQKLPKEQQEIMKQQMLPLNKRLFLHHKWDLILNQKLEAGLDVSAGLLEILRTEIKKHHDLAGTQFQKEQLQETFAEFVKSVAERPPRNDLLGTNAKSEVVTLCAEKEDGTARVTKFMLVPGIDVFKPEEYYDDADDIDRKRFNNEIDKETYKIAREEATSQNNFMNSAIKFKYYGKIKR

Target ORF information:

RefSeq Version	NM_060324.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans EGF-like domain-containing protein (F25D7.5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_060324.3

ORF Insert Sequence:

ATGCGGAACA AATATATTTT CTCCATGTTT CCTATTATTT TGCTGGTTTT CTTTGGAAAA 
CTAGAAAGTA CTGATCGAAT GTCAACGAAT ACGATTCCAA TTGAACCTTC TACTCCTACA 
ACAACACCTT CTGATATTGT GCTCCCAGAA AACACTACTA TGTCCGATAT ATTAAAAAAT 
ACTAAAAGCG GGCAATGTGA AACATTGGAA GAAGTTCTGT GTGGATGTTC GACATGTTCA 
ACATCATGTC TTGGAACGTC AACGACAGTA GGATCTTCCA AGCGGAGTTC AATTTCCACT 
GTGACCAAAA ATGAAACGTC CACTAGATCA TCGTCATCTA CTTCGCAGAG CTTACCAACA 
AGCAAAAGTT TGGTTTCGAG CACCACATCC AACAAAGATC TTGAAACAAC TTCTGCTACG 
ATTACGGTCT CATCTAGCTC CATCACCAGC TCCACCAAAG AAATCTCAAG TAGAACAACT 
GGAAACTCAT CTGGAACTTC TGAAGTCACT TCAACTTTCT CTAAGGTGCC ACTGGCTACA 
CAATCCTCTC ACTCTACATC CTTGCCGTCA ACCCCATCTA CTTCAACTCA ACCAAGTTCA 
ACATCTGCCA AAGGCTCCAC AACATCCACT ATTGGTGATT TGAACACAAC TCGGACATCG 
ACGATTTCAA GCACAGAAAA ATCTAGCTCA ATGTCCCCAA CAATCAAAAG CTCATCTCCC 
TCCTCACCAC CTATGACACC CACAATTAAG ATTTCAACAC AGAAAGGATT ATCTTCTAGC 
ACAGTCAGTT TTCCGAGATG TTTCGTTTTC TATCAAAAAT CAACCTACAT TTCAACAAGA 
ACTGAGTCAA CCACCGTATC ACCGACATCA TCAAGTACTG CAATACAGGA TAATACAACA 
TTCTCCACAG CAAAATCCAC TGCAACTTCA AGCCAAACTC CAATTCATTC GACTTCAGCA 
ACCCTCGCAA GCAGTACCGT AAAATCTTCA TCTCCTGCTT CATCAGGATC TACTAGTTCC 
AGCTCCACCT CACAGCCTTC GACATCGTCA AAAAACACAG ATTCGTCAAC TGCACCTTGG 
ACTGAGCACG CAAAAGCTGA AGATATAATT TCAAGTACTA CTACTTCTAA AATAAGTTCA 
TCTTTGTCAT CTACCCAGTC TACAGCTTCA ACTTCAAGCA CTCCATATTC CACCTCGTCT 
TCAGAAACTC CAGATTCTTC TACCACCACG ACTGTGATAT CCACCACTAG AGATCCATCT 
TGTTCGGTGC AATGTCCTGA TGGATATGTT GCAGGTGAAA CAATGTGCTA CTACTTTGCC 
GAACTATCTG ACATCAAGTC CTATCAATCA GCTCTTTCTT ACTGCCAAAC AGTCAATCTT 
CGGAACCTGA TCTTTCTTGA GAATCTTCGA GATCTCAATG ATCTTCAAAC TCTTCGCTCA 
AAAAGTTCAT CAATTCAATC CACGTCGTTC TATTTTGCAA ACGGAGGAGG ATCTGTGCAA 
GAGCGGCTCA ACAAAAAAGC TCAAGTACTT GATATTACCC AATTAACAGC CATGACATCT 
CCAATTGCAA TGACAGTGGG GATATCTGAC ACTACGACGA ATGTTTCAGC GATATGTGTT 
CTTCCACAAT ACTGTAACAT ATCCAAATGC TACGTGGAAA CTGCTCTAAA CATCCTCGAA 
CTCAACTCAA TTCTCTCTTC ATCAAAAGAA ACAATTGATA CTACTTCCAC TGCAAGCCTA 
ACATGCTCCT ACACAAAATC AATTACAACT GTCTCATGTG GAACACGTGG AAATATAATG 
CCAAGTCCAA CAACTATCAG TTGTGCAGCA GAAGGTGTTG CTCAGAAGCT TGCAAATACA 
ACGTCTGATT TTGTCTCTAG TTGCGCTATG TGTTTTACTC GTGGAACTGA CTTCTGTGAG 
ACAACAGTTG ATGCAGATGG AAATTATGCC TATCAATGTC ATTGCAAGCC TAAATATTCT 
ATGAGCACCT GCTGGTACAC TCCTGATGCT TGCACTCCAA CTACATGTAA TGGTCATGGA 
AAGTGTTATG ATTACGTAGA AGATGTGAAA TGTGACTGTT ACTGGGGATA CGAGGGCGAA 
CATTGTGAAG TGAATAAGGA TCGAGTCACA AATACAACTG ACTCATTTTT CACTCGTTTT 
GGAAATTATA TTTTCGATTC TCCGACTATC AATTCTCTCG TGTCCTGTTC TTCGACAGTG 
CTCCTGATCG GTGTTAAATC TGTTGGAAAA ATTTACTTGT CGAATGGAGA AGATGATCCA 
CAAGAAACTC ATCAAACGGC TCGATCATTT TTATTGACTC TCGGCACATC CTGCGTACTC 
TTTTTCAACG ATCCGTATAT GTTCAAAATC TCACAAGCCA CGTGTCGGAT GTTCTTTATC 
GTTATACATT TTTGTTTCAT TGGAGCAATG ATCCAGTGGA TGCTAGAAGG ATATAATGCA 
AATCAAGTGG TTCGATGTGT TCATCTAAAT GAATGGGAAC GAGATTTTGA ATGTAGAAGA 
GCATGGGGTA TAATGGTTGC TCCACGAATG GTTGTACCAT TGCTTTTATT GGCAGTAGCA 
CTGGGTGTCC TTTTTCAATC AAACTGGAAT CAACTTCCTT CAACATGGAC ATGCGTTGGT 
GTGATTTGCA ATGAAACAAC TTCAGTTTGG TTATCAGCAA TATGGATTGT TCTTTGTATT 
GTTGTCTCTA CGGCAACATT TGCAGAATCC AGTGTTTTAA TCAAAAATCG CCGTCCCTTG 
TACAATCTGA AAATTCGACA ACGAATCGAA CATAAGGAGG GAGTCATTGA TGGTTGGGTT 
GCCGAAAAGT GCCGAAGAAA TACAGTTTTA TGTTTTATTG GAGTTTCTCT TCTGTCTGCA 
ACGTGGCTCC TGACCATTCT AGCCGCGGAT AAACGGAGCA AAGGAATTTA TGGATGGGTT 
CTCACACTCG TGGGAATCAT TTATGGAGTA TTCAGTTTTG TCCAAGGACT CTACACGGAT 
CCAAATTGTT GGTCCTCTAT TCTATGGCTC GCGATGAAAA AGTTTCCTGC TCGATTTGCT 
CCATCCTATG ATCCGATTTC CATGTGGACT CGTGAGGAAG TGAAAGAAAT TCAAAAGCTT 
CCAAAAGAAC AACAAGAAAT AATGAAACAA CAAATGCTTC CATTGAATAA AAGATTATTC 
CTACATCACA AATGGGATTT GATTCTCAAT CAGAAATTGG AAGCAGGACT GGATGTCTCT 
GCTGGTTTAC TGGAAATTCT GCGAACTGAG ATAAAGAAGC ATCATGATTT GGCTGGAACT 
CAATTTCAAA AAGAACAACT CCAAGAAACA TTTGCGGAAT TTGTGAAATC AGTCGCTGAA 
CGACCACCAA GAAATGATTT ATTGGGAACC AATGCAAAGA GTGAAGTTGT GACTCTATGT 
GCAGAAAAAG AGGATGGAAC TGCAAGAGTG ACCAAATTTA TGCTAGTCCC CGGAATCGAT 
GTTTTCAAAC CAGAAGAGTA CTATGATGAT GCTGATGATA TTGACAGAAA GAGATTTAAT 
AATGAAATTG ATAAGGAAAC CTACAAAATT GCAAGAGAAG AAGCAACGTC TCAAAACAAC 
TTTATGAATT CGGCCATCAA ATTCAAGTAC TACGGAAAAA TCAAAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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F25D7.5 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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