nrde-3 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	nrde-3
Entrez Gene ID	180392
Full Name	Nuclear RNAi defective-3 protein
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_075691.4	NP_508092.1	Nuclear RNAi defective-3 protein
NM_075691.5	NP_508092.1	Nuclear RNAi defective-3 protein

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	wago-9	NP_497834.1
Caenorhabditis elegans (roundworm)	wago-11	NP_494593.1
Caenorhabditis elegans (roundworm)	nrde-3	NP_508092.1
Caenorhabditis elegans (roundworm)	C14B1.7	NP_001254877.1
Caenorhabditis elegans (roundworm)	wago-10	NP_503177.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

nrde-3 contributes to transitive RNAi, whereby amplified silencing triggers act in trans to silence sequence-similar genes.
Title: The nuclear argonaute NRDE-3 contributes to transitive RNAi in Caenorhabditis elegans.

Studies indicate that the heritable silencing pathway depends on at least four genes, designated as nuclear RNAi defective 1-4 (nrde-1/2/3/4).
Title: Small RNAs, RNAi and the inheritance of gene silencing in Caenorhabditis elegans.

NRDE-3 binds siRNAs generated by RNA-dependent RNA polymerases acting on messenger RNA templates in the cytoplasm and redistributes to the nucleus
Title: An Argonaute transports siRNAs from the cytoplasm to the nucleus.

The following nrde-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nrde-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa349181	NM_075691.5 Latest version!	Caenorhabditis elegans Nuclear RNAi defective-3 protein (nrde-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa349181	NM_075691.4	Caenorhabditis elegans Nuclear RNAi defective-3 protein (nrde-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nrde-3 ( NM_075691.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_508092.1 Caenorhabditis elegans Nuclear RNAi defective-3 protein (nrde-3), partial mRNA.

Price & Availability↑

CloneID	OCa349181
Clone ID Related Accession (Same CDS sequence)	NM_075691.4 , NM_075691.5
Accession Version	NM_075691.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Nuclear RNAi defective-3 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_075691.3. COMPLETENESS: incomplete on both ends.

ATGGATCTCC TAGACAAAGT AATGGGTGAG ATGGGCTCTA AGCCAGGCTC AACTGCCAAA 
AAACCAGCCA CATCTGCGTC ATCCACACCA CGTACAAATG TTTGGGGCAC TGCAAAAAAG 
CCATCCAGCC AACAGCAGCC ACCGAAGCCA CTTTTCACTA CTCCGGGCTC ACAGCAAGGA 
TCTCTTGGAG GTCGGATTCC AAAAAGAGAG CATACCGATC GCACAGGTCC CGATCCAAAA 
CGAAAGCCAC TGGGAGGATT GTCGGTGCCG GATTCATTCA ATAATTTTGG TACGTTTCGG 
GTACAAATGA ATGCTTGGAA TTTAGATATT TCTAAAATGG ACGAGAGAAT TAGCAGAATA 
ATGTTTCGTG CCACATTGGT TCACACGGAT GGACGTCGCT TTGAACTTTC TCTTGGCGTT 
TCGGCATTCT CCGGAGACGT CAACCGTCAA CAAAGACGTC AAGCTCAATG CCTGCTCTTC 
CGGGCATGGT TCAAACGAAA TCCTGAGCTG TTCAAAGGTA TGACTGATCC GGCAATCGCT 
GCATACGACG CTGCGGAAAC TATCTATGTG GGATGTAGCT TCTTTGACGT CGAGTTGACC 
GAGCACGTAT GCCATCTGAC AGAAGCTGAC TTTTCGCCAC AAGAATGGAA AATCGTTTCT 
CTCATCTCAC GTCGGAGTGG GTCGACATTT GAAATTCGCA TCAAGACAAA TCCACCAATC 
TACACGCGTG GACCAAACGC TTTGACATTG GAGAACCGTT CTGAGCTAAC ACGTATCATT 
GAAGCTATAA CAGACCAATG TCTTCACAAT GAGAAGTTTT TGCTCTATTC GTCGGGAACT 
TTCCCCACGA AGGGGGGCGA TATTGCCAGT CCCGACGAGG TTACTCTGAT TAAGTCAGGA 
TTCGTGAAGA CTACAAAAAT TGTGGATCGG GACGGTGTAC CAGATGCAAT TATGACCGTT 
GACACAACAA AATCGCCATT CTACAAAGAT ACGTCTCTCC TCAAATTTTT CACTGCCAAA 
ATGGATCAAC TTACAAATAG TGGTGGAGGA CCCAGAGGCC ACAATGGTGG CAGAGAGCGT 
CGCGATGGCG GCGGGAACAG TAGAAAATAC GATGATAGAC GCTCACCGCG TGATGGTGAA 
ATTGATTATG ACGAGAGAAC TGTCTCACAT TATCAAAGAC AGTTTCAAGA CGAACGCATT 
TCGGATGGTA TGCTGAACAC TCTCAAGCAG TCTCTCAAAG GATTGGATTG TCAGCCGATT 
CATCTTAAAG ACTCGAAAGC CAATCGTTCA ATCATGATAG ACGAAATTCA CACCGGAACA 
GCAGATAGTG TCACATTTGA ACAAAAACTT CCCGACGGAG AGATGAAGCT CACTTCCATC 
ACAGAGTATT ATTTACAACG GTACAACTAT CGTCTCAAAT TCCCACATCT TCCGTTGGTG 
ACTTCAAAAC GGGCGAAATG TTACGATTTC TATCCTATGG AACTCATGTC CATTCTTCCT 
GGCCAGAGAA TCAAACAGTC ACATATGACT GTTGATATTC AATCTTATAT GACTGGAAAA 
ATGAGCAGCC TGCCTGATCA GCACATCAAA CAGTCGAAAT TGGTTTTGAC GGAATATTTA 
AAACTTGGTG ACCAGCCTGC AAATCGCCAA ATGGATGCTT TCCGTGTGTC GCTCAAAAGC 
ATCCAGCCAA TTGTCACGAA TGCCCATTGG CTCTCCCCAC CAGACATGAA GTTCGCAAAC 
AATCAGCTGT ACAGTCTAAA TCCAACCCGT GGTGTGCGTT TCCAGACTAA TGGCAAGTTT 
GTCATGCCAG CCAGAGTAAA GTCGGTGACG ATTATCAACT ACGACAAGGA ATTTAATAGA 
AACGTCGATA TGTTTGCCGA AGGGCTTGCC AAGCATTGCT CCGAACAAGG AATGAAGTTT 
GATAGCCGCC CGAACAGCTG GAAAAAAGTT AATCTCGGCT CATCAGACCG ACGAGGTACA 
AAAGTGGAGA TAGAAGAAGC CATTCGAAAC GGCGTTACGA TTGTGTTTGG AATTATTGCC 
GAAAAACGAC CAGATATGCA TGACATTCTC AAATACTTTG AGGAGAAGCT TGGACAGCAA 
ACCATTCAAA TTTCTTCTGA AACCGCGGAC AAATTCATGC GTGACCATGG TGGAAAACAA 
ACCATTGACA ATGTCATCAG AAAATTGAAC CCCAAGTGTG GAGGAACTAA CTTCCTAATT 
GACGTGCCCG AGTCGGTTGG CCATCGAGTC GTTTGCAACA ATTCTGCTGA AATGAGAGCA 
AAACTGTACG CAAAGACGCA ATTTATCGGA TTCGAGATGT CGCATACAGG CGCTAGGACC 
AGATTCGACA TTCAGAAAGT TATGTTTGAC GGAGATCCAA CTGTCGTCGG AGTTGCGTAC 
TCTCTCAAAC ACAGTGCTCA GCTTGGAGGG TTTTCATATT TCCAGGAAAG TCGTTTGCAC 
AAGCTCACAA ATCTTCAAGA GAAGATGCAA ATTTGCTTGA ATGCTTACGA GCAATCGAGC 
TCGTATCTTC CTGAGACAGT TGTTGTCTAT CGTGTCGGAA GTGGTGAAGG AGATTATCCG 
CAAATTGTCA ACGAGGTTAA CGAGATGAAG CTGGCTGCAA GAAAGAAGAA GCATGGTTAC 
AATCCCAAGT TTCTCGTTAT TTGCACACAA CGCAACTCTC ACATCCGAGT ATTCCCTGAA 
CACATTAATG AACGTGGAAA GTCCATGGAG CAAAATGTAA AATCCGGCAC TTGCGTCGAT 
GTGCCAGGAG CCTCTCACGG ATACGAGGAG TTCATTCTTT GTTGCCAAAC ACCTCTGATT 
GGAACAGTAA AACCAACAAA GTATACTATC ATTGTCAACG ATTGTCGTTG GAGCAAGAAT 
GAGATCATGA ATGTCACCTA CCACTTGGCG TTTGCCCACC AGGTCTCTTA TGCACCACCA 
GCTATCCCCA ACGTGAGCTA TGCTGCTCAA AATCTTGCTA AACGTGGTCA CAACAACTAC 
AAAACACACA CAAAGCTTGT GGACATGAAC GACTACTCTT ATCGAATCAA GGAAAAACAT 
GAGGAAATCA TTTCGTCAGA GGAAGTTGAC GATATTCTGA TGCGCGATTT CATTGAGACA 
GTTTCCAACG ATCTAAATGC AATGACCATC AACGGACGCA ACTTTTGGGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_508092.1
CDS	1..3174
Translation	MDLLDKVMGEMGSKPGSTAKKPATSASSTPRTNVWGTAKKPSSQQQPPKPLFTTPGSQQGSLGGRIPKREHTDRTGPDPKRKPLGGLSVPDSFNNFGTFRVQMNAWNLDISKMDERISRIMFRATLVHTDGRRFELSLGVSAFSGDVNRQQRRQAQCLLFRAWFKRNPELFKGMTDPAIAAYDAAETIYVGCSFFDVELTEHVCHLTEADFSPQEWKIVSLISRRSGSTFEIRIKTNPPIYTRGPNALTLENRSELTRIIEAITDQCLHNEKFLLYSSGTFPTKGGDIASPDEVTLIKSGFVKTTKIVDRDGVPDAIMTVDTTKSPFYKDTSLLKFFTAKMDQLTNSGGGPRGHNGGRERRDGGGNSRKYDDRRSPRDGEIDYDERTVSHYQRQFQDERISDGMLNTLKQSLKGLDCQPIHLKDSKANRSIMIDEIHTGTADSVTFEQKLPDGEMKLTSITEYYLQRYNYRLKFPHLPLVTSKRAKCYDFYPMELMSILPGQRIKQSHMTVDIQSYMTGKMSSLPDQHIKQSKLVLTEYLKLGDQPANRQMDAFRVSLKSIQPIVTNAHWLSPPDMKFANNQLYSLNPTRGVRFQTNGKFVMPARVKSVTIINYDKEFNRNVDMFAEGLAKHCSEQGMKFDSRPNSWKKVNLGSSDRRGTKVEIEEAIRNGVTIVFGIIAEKRPDMHDILKYFEEKLGQQTIQISSETADKFMRDHGGKQTIDNVIRKLNPKCGGTNFLIDVPESVGHRVVCNNSAEMRAKLYAKTQFIGFEMSHTGARTRFDIQKVMFDGDPTVVGVAYSLKHSAQLGGFSYFQESRLHKLTNLQEKMQICLNAYEQSSSYLPETVVVYRVGSGEGDYPQIVNEVNEMKLAARKKKHGYNPKFLVICTQRNSHIRVFPEHINERGKSMEQNVKSGTCVDVPGASHGYEEFILCCQTPLIGTVKPTKYTIIVNDCRWSKNEIMNVTYHLAFAHQVSYAPPAIPNVSYAAQNLAKRGHNNYKTHTKLVDMNDYSYRIKEKHEEIISSEEVDDILMRDFIETVSNDLNAMTINGRNFWA

Target ORF information:

RefSeq Version	NM_075691.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Nuclear RNAi defective-3 protein (nrde-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_075691.4

ORF Insert Sequence:

ATGGATCTCC TAGACAAAGT AATGGGTGAG ATGGGCTCTA AGCCAGGCTC AACTGCCAAA 
AAACCAGCCA CATCTGCGTC ATCCACACCA CGTACAAATG TTTGGGGCAC TGCAAAAAAG 
CCATCCAGCC AACAGCAGCC ACCGAAGCCA CTTTTCACTA CTCCGGGCTC ACAGCAAGGA 
TCTCTTGGAG GTCGGATTCC AAAAAGAGAG CATACCGATC GCACAGGTCC CGATCCAAAA 
CGAAAGCCAC TGGGAGGATT GTCGGTGCCG GATTCATTCA ATAATTTTGG TACGTTTCGG 
GTACAAATGA ATGCTTGGAA TTTAGATATT TCTAAAATGG ACGAGAGAAT TAGCAGAATA 
ATGTTTCGTG CCACATTGGT TCACACGGAT GGACGTCGCT TTGAACTTTC TCTTGGCGTT 
TCGGCATTCT CCGGAGACGT CAACCGTCAA CAAAGACGTC AAGCTCAATG CCTGCTCTTC 
CGGGCATGGT TCAAACGAAA TCCTGAGCTG TTCAAAGGTA TGACTGATCC GGCAATCGCT 
GCATACGACG CTGCGGAAAC TATCTATGTG GGATGTAGCT TCTTTGACGT CGAGTTGACC 
GAGCACGTAT GCCATCTGAC AGAAGCTGAC TTTTCGCCAC AAGAATGGAA AATCGTTTCT 
CTCATCTCAC GTCGGAGTGG GTCGACATTT GAAATTCGCA TCAAGACAAA TCCACCAATC 
TACACGCGTG GACCAAACGC TTTGACATTG GAGAACCGTT CTGAGCTAAC ACGTATCATT 
GAAGCTATAA CAGACCAATG TCTTCACAAT GAGAAGTTTT TGCTCTATTC GTCGGGAACT 
TTCCCCACGA AGGGGGGCGA TATTGCCAGT CCCGACGAGG TTACTCTGAT TAAGTCAGGA 
TTCGTGAAGA CTACAAAAAT TGTGGATCGG GACGGTGTAC CAGATGCAAT TATGACCGTT 
GACACAACAA AATCGCCATT CTACAAAGAT ACGTCTCTCC TCAAATTTTT CACTGCCAAA 
ATGGATCAAC TTACAAATAG TGGTGGAGGA CCCAGAGGCC ACAATGGTGG CAGAGAGCGT 
CGCGATGGCG GCGGGAACAG TAGAAAATAC GATGATAGAC GCTCACCGCG TGATGGTGAA 
ATTGATTATG ACGAGAGAAC TGTCTCACAT TATCAAAGAC AGTTTCAAGA CGAACGCATT 
TCGGATGGTA TGCTGAACAC TCTCAAGCAG TCTCTCAAAG GATTGGATTG TCAGCCGATT 
CATCTTAAAG ACTCGAAAGC CAATCGTTCA ATCATGATAG ACGAAATTCA CACCGGAACA 
GCAGATAGTG TCACATTTGA ACAAAAACTT CCCGACGGAG AGATGAAGCT CACTTCCATC 
ACAGAGTATT ATTTACAACG GTACAACTAT CGTCTCAAAT TCCCACATCT TCCGTTGGTG 
ACTTCAAAAC GGGCGAAATG TTACGATTTC TATCCTATGG AACTCATGTC CATTCTTCCT 
GGCCAGAGAA TCAAACAGTC ACATATGACT GTTGATATTC AATCTTATAT GACTGGAAAA 
ATGAGCAGCC TGCCTGATCA GCACATCAAA CAGTCGAAAT TGGTTTTGAC GGAATATTTA 
AAACTTGGTG ACCAGCCTGC AAATCGCCAA ATGGATGCTT TCCGTGTGTC GCTCAAAAGC 
ATCCAGCCAA TTGTCACGAA TGCCCATTGG CTCTCCCCAC CAGACATGAA GTTCGCAAAC 
AATCAGCTGT ACAGTCTAAA TCCAACCCGT GGTGTGCGTT TCCAGACTAA TGGCAAGTTT 
GTCATGCCAG CCAGAGTAAA GTCGGTGACG ATTATCAACT ACGACAAGGA ATTTAATAGA 
AACGTCGATA TGTTTGCCGA AGGGCTTGCC AAGCATTGCT CCGAACAAGG AATGAAGTTT 
GATAGCCGCC CGAACAGCTG GAAAAAAGTT AATCTCGGCT CATCAGACCG ACGAGGTACA 
AAAGTGGAGA TAGAAGAAGC CATTCGAAAC GGCGTTACGA TTGTGTTTGG AATTATTGCC 
GAAAAACGAC CAGATATGCA TGACATTCTC AAATACTTTG AGGAGAAGCT TGGACAGCAA 
ACCATTCAAA TTTCTTCTGA AACCGCGGAC AAATTCATGC GTGACCATGG TGGAAAACAA 
ACCATTGACA ATGTCATCAG AAAATTGAAC CCCAAGTGTG GAGGAACTAA CTTCCTAATT 
GACGTGCCCG AGTCGGTTGG CCATCGAGTC GTTTGCAACA ATTCTGCTGA AATGAGAGCA 
AAACTGTACG CAAAGACGCA ATTTATCGGA TTCGAGATGT CGCATACAGG CGCTAGGACC 
AGATTCGACA TTCAGAAAGT TATGTTTGAC GGAGATCCAA CTGTCGTCGG AGTTGCGTAC 
TCTCTCAAAC ACAGTGCTCA GCTTGGAGGG TTTTCATATT TCCAGGAAAG TCGTTTGCAC 
AAGCTCACAA ATCTTCAAGA GAAGATGCAA ATTTGCTTGA ATGCTTACGA GCAATCGAGC 
TCGTATCTTC CTGAGACAGT TGTTGTCTAT CGTGTCGGAA GTGGTGAAGG AGATTATCCG 
CAAATTGTCA ACGAGGTTAA CGAGATGAAG CTGGCTGCAA GAAAGAAGAA GCATGGTTAC 
AATCCCAAGT TTCTCGTTAT TTGCACACAA CGCAACTCTC ACATCCGAGT ATTCCCTGAA 
CACATTAATG AACGTGGAAA GTCCATGGAG CAAAATGTAA AATCCGGCAC TTGCGTCGAT 
GTGCCAGGAG CCTCTCACGG ATACGAGGAG TTCATTCTTT GTTGCCAAAC ACCTCTGATT 
GGAACAGTAA AACCAACAAA GTATACTATC ATTGTCAACG ATTGTCGTTG GAGCAAGAAT 
GAGATCATGA ATGTCACCTA CCACTTGGCG TTTGCCCACC AGGTCTCTTA TGCACCACCA 
GCTATCCCCA ACGTGAGCTA TGCTGCTCAA AATCTTGCTA AACGTGGTCA CAACAACTAC 
AAAACACACA CAAAGCTTGT GGACATGAAC GACTACTCTT ATCGAATCAA GGAAAAACAT 
GAGGAAATCA TTTCGTCAGA GGAAGTTGAC GATATTCTGA TGCGCGATTT CATTGAGACA 
GTTTCCAACG ATCTAAATGC AATGACCATC AACGGACGCA ACTTTTGGGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrde-3 ( NM_075691.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_508092.1 Caenorhabditis elegans Nuclear RNAi defective-3 protein (nrde-3), mRNA.

Price & Availability↑

CloneID	OCa349181
Clone ID Related Accession (Same CDS sequence)	NM_075691.4 , NM_075691.5
Accession Version	NM_075691.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Nuclear RNAi defective-3 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_075691.4.

ATGGATCTCC TAGACAAAGT AATGGGTGAG ATGGGCTCTA AGCCAGGCTC AACTGCCAAA 
AAACCAGCCA CATCTGCGTC ATCCACACCA CGTACAAATG TTTGGGGCAC TGCAAAAAAG 
CCATCCAGCC AACAGCAGCC ACCGAAGCCA CTTTTCACTA CTCCGGGCTC ACAGCAAGGA 
TCTCTTGGAG GTCGGATTCC AAAAAGAGAG CATACCGATC GCACAGGTCC CGATCCAAAA 
CGAAAGCCAC TGGGAGGATT GTCGGTGCCG GATTCATTCA ATAATTTTGG TACGTTTCGG 
GTACAAATGA ATGCTTGGAA TTTAGATATT TCTAAAATGG ACGAGAGAAT TAGCAGAATA 
ATGTTTCGTG CCACATTGGT TCACACGGAT GGACGTCGCT TTGAACTTTC TCTTGGCGTT 
TCGGCATTCT CCGGAGACGT CAACCGTCAA CAAAGACGTC AAGCTCAATG CCTGCTCTTC 
CGGGCATGGT TCAAACGAAA TCCTGAGCTG TTCAAAGGTA TGACTGATCC GGCAATCGCT 
GCATACGACG CTGCGGAAAC TATCTATGTG GGATGTAGCT TCTTTGACGT CGAGTTGACC 
GAGCACGTAT GCCATCTGAC AGAAGCTGAC TTTTCGCCAC AAGAATGGAA AATCGTTTCT 
CTCATCTCAC GTCGGAGTGG GTCGACATTT GAAATTCGCA TCAAGACAAA TCCACCAATC 
TACACGCGTG GACCAAACGC TTTGACATTG GAGAACCGTT CTGAGCTAAC ACGTATCATT 
GAAGCTATAA CAGACCAATG TCTTCACAAT GAGAAGTTTT TGCTCTATTC GTCGGGAACT 
TTCCCCACGA AGGGGGGCGA TATTGCCAGT CCCGACGAGG TTACTCTGAT TAAGTCAGGA 
TTCGTGAAGA CTACAAAAAT TGTGGATCGG GACGGTGTAC CAGATGCAAT TATGACCGTT 
GACACAACAA AATCGCCATT CTACAAAGAT ACGTCTCTCC TCAAATTTTT CACTGCCAAA 
ATGGATCAAC TTACAAATAG TGGTGGAGGA CCCAGAGGCC ACAATGGTGG CAGAGAGCGT 
CGCGATGGCG GCGGGAACAG TAGAAAATAC GATGATAGAC GCTCACCGCG TGATGGTGAA 
ATTGATTATG ACGAGAGAAC TGTCTCACAT TATCAAAGAC AGTTTCAAGA CGAACGCATT 
TCGGATGGTA TGCTGAACAC TCTCAAGCAG TCTCTCAAAG GATTGGATTG TCAGCCGATT 
CATCTTAAAG ACTCGAAAGC CAATCGTTCA ATCATGATAG ACGAAATTCA CACCGGAACA 
GCAGATAGTG TCACATTTGA ACAAAAACTT CCCGACGGAG AGATGAAGCT CACTTCCATC 
ACAGAGTATT ATTTACAACG GTACAACTAT CGTCTCAAAT TCCCACATCT TCCGTTGGTG 
ACTTCAAAAC GGGCGAAATG TTACGATTTC TATCCTATGG AACTCATGTC CATTCTTCCT 
GGCCAGAGAA TCAAACAGTC ACATATGACT GTTGATATTC AATCTTATAT GACTGGAAAA 
ATGAGCAGCC TGCCTGATCA GCACATCAAA CAGTCGAAAT TGGTTTTGAC GGAATATTTA 
AAACTTGGTG ACCAGCCTGC AAATCGCCAA ATGGATGCTT TCCGTGTGTC GCTCAAAAGC 
ATCCAGCCAA TTGTCACGAA TGCCCATTGG CTCTCCCCAC CAGACATGAA GTTCGCAAAC 
AATCAGCTGT ACAGTCTAAA TCCAACCCGT GGTGTGCGTT TCCAGACTAA TGGCAAGTTT 
GTCATGCCAG CCAGAGTAAA GTCGGTGACG ATTATCAACT ACGACAAGGA ATTTAATAGA 
AACGTCGATA TGTTTGCCGA AGGGCTTGCC AAGCATTGCT CCGAACAAGG AATGAAGTTT 
GATAGCCGCC CGAACAGCTG GAAAAAAGTT AATCTCGGCT CATCAGACCG ACGAGGTACA 
AAAGTGGAGA TAGAAGAAGC CATTCGAAAC GGCGTTACGA TTGTGTTTGG AATTATTGCC 
GAAAAACGAC CAGATATGCA TGACATTCTC AAATACTTTG AGGAGAAGCT TGGACAGCAA 
ACCATTCAAA TTTCTTCTGA AACCGCGGAC AAATTCATGC GTGACCATGG TGGAAAACAA 
ACCATTGACA ATGTCATCAG AAAATTGAAC CCCAAGTGTG GAGGAACTAA CTTCCTAATT 
GACGTGCCCG AGTCGGTTGG CCATCGAGTC GTTTGCAACA ATTCTGCTGA AATGAGAGCA 
AAACTGTACG CAAAGACGCA ATTTATCGGA TTCGAGATGT CGCATACAGG CGCTAGGACC 
AGATTCGACA TTCAGAAAGT TATGTTTGAC GGAGATCCAA CTGTCGTCGG AGTTGCGTAC 
TCTCTCAAAC ACAGTGCTCA GCTTGGAGGG TTTTCATATT TCCAGGAAAG TCGTTTGCAC 
AAGCTCACAA ATCTTCAAGA GAAGATGCAA ATTTGCTTGA ATGCTTACGA GCAATCGAGC 
TCGTATCTTC CTGAGACAGT TGTTGTCTAT CGTGTCGGAA GTGGTGAAGG AGATTATCCG 
CAAATTGTCA ACGAGGTTAA CGAGATGAAG CTGGCTGCAA GAAAGAAGAA GCATGGTTAC 
AATCCCAAGT TTCTCGTTAT TTGCACACAA CGCAACTCTC ACATCCGAGT ATTCCCTGAA 
CACATTAATG AACGTGGAAA GTCCATGGAG CAAAATGTAA AATCCGGCAC TTGCGTCGAT 
GTGCCAGGAG CCTCTCACGG ATACGAGGAG TTCATTCTTT GTTGCCAAAC ACCTCTGATT 
GGAACAGTAA AACCAACAAA GTATACTATC ATTGTCAACG ATTGTCGTTG GAGCAAGAAT 
GAGATCATGA ATGTCACCTA CCACTTGGCG TTTGCCCACC AGGTCTCTTA TGCACCACCA 
GCTATCCCCA ACGTGAGCTA TGCTGCTCAA AATCTTGCTA AACGTGGTCA CAACAACTAC 
AAAACACACA CAAAGCTTGT GGACATGAAC GACTACTCTT ATCGAATCAA GGAAAAACAT 
GAGGAAATCA TTTCGTCAGA GGAAGTTGAC GATATTCTGA TGCGCGATTT CATTGAGACA 
GTTTCCAACG ATCTAAATGC AATGACCATC AACGGACGCA ACTTTTGGGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_508092.1
CDS	20..3193
Translation	MDLLDKVMGEMGSKPGSTAKKPATSASSTPRTNVWGTAKKPSSQQQPPKPLFTTPGSQQGSLGGRIPKREHTDRTGPDPKRKPLGGLSVPDSFNNFGTFRVQMNAWNLDISKMDERISRIMFRATLVHTDGRRFELSLGVSAFSGDVNRQQRRQAQCLLFRAWFKRNPELFKGMTDPAIAAYDAAETIYVGCSFFDVELTEHVCHLTEADFSPQEWKIVSLISRRSGSTFEIRIKTNPPIYTRGPNALTLENRSELTRIIEAITDQCLHNEKFLLYSSGTFPTKGGDIASPDEVTLIKSGFVKTTKIVDRDGVPDAIMTVDTTKSPFYKDTSLLKFFTAKMDQLTNSGGGPRGHNGGRERRDGGGNSRKYDDRRSPRDGEIDYDERTVSHYQRQFQDERISDGMLNTLKQSLKGLDCQPIHLKDSKANRSIMIDEIHTGTADSVTFEQKLPDGEMKLTSITEYYLQRYNYRLKFPHLPLVTSKRAKCYDFYPMELMSILPGQRIKQSHMTVDIQSYMTGKMSSLPDQHIKQSKLVLTEYLKLGDQPANRQMDAFRVSLKSIQPIVTNAHWLSPPDMKFANNQLYSLNPTRGVRFQTNGKFVMPARVKSVTIINYDKEFNRNVDMFAEGLAKHCSEQGMKFDSRPNSWKKVNLGSSDRRGTKVEIEEAIRNGVTIVFGIIAEKRPDMHDILKYFEEKLGQQTIQISSETADKFMRDHGGKQTIDNVIRKLNPKCGGTNFLIDVPESVGHRVVCNNSAEMRAKLYAKTQFIGFEMSHTGARTRFDIQKVMFDGDPTVVGVAYSLKHSAQLGGFSYFQESRLHKLTNLQEKMQICLNAYEQSSSYLPETVVVYRVGSGEGDYPQIVNEVNEMKLAARKKKHGYNPKFLVICTQRNSHIRVFPEHINERGKSMEQNVKSGTCVDVPGASHGYEEFILCCQTPLIGTVKPTKYTIIVNDCRWSKNEIMNVTYHLAFAHQVSYAPPAIPNVSYAAQNLAKRGHNNYKTHTKLVDMNDYSYRIKEKHEEIISSEEVDDILMRDFIETVSNDLNAMTINGRNFWA

Target ORF information:

RefSeq Version	NM_075691.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Nuclear RNAi defective-3 protein (nrde-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_075691.5

ORF Insert Sequence:

ATGGATCTCC TAGACAAAGT AATGGGTGAG ATGGGCTCTA AGCCAGGCTC AACTGCCAAA 
AAACCAGCCA CATCTGCGTC ATCCACACCA CGTACAAATG TTTGGGGCAC TGCAAAAAAG 
CCATCCAGCC AACAGCAGCC ACCGAAGCCA CTTTTCACTA CTCCGGGCTC ACAGCAAGGA 
TCTCTTGGAG GTCGGATTCC AAAAAGAGAG CATACCGATC GCACAGGTCC CGATCCAAAA 
CGAAAGCCAC TGGGAGGATT GTCGGTGCCG GATTCATTCA ATAATTTTGG TACGTTTCGG 
GTACAAATGA ATGCTTGGAA TTTAGATATT TCTAAAATGG ACGAGAGAAT TAGCAGAATA 
ATGTTTCGTG CCACATTGGT TCACACGGAT GGACGTCGCT TTGAACTTTC TCTTGGCGTT 
TCGGCATTCT CCGGAGACGT CAACCGTCAA CAAAGACGTC AAGCTCAATG CCTGCTCTTC 
CGGGCATGGT TCAAACGAAA TCCTGAGCTG TTCAAAGGTA TGACTGATCC GGCAATCGCT 
GCATACGACG CTGCGGAAAC TATCTATGTG GGATGTAGCT TCTTTGACGT CGAGTTGACC 
GAGCACGTAT GCCATCTGAC AGAAGCTGAC TTTTCGCCAC AAGAATGGAA AATCGTTTCT 
CTCATCTCAC GTCGGAGTGG GTCGACATTT GAAATTCGCA TCAAGACAAA TCCACCAATC 
TACACGCGTG GACCAAACGC TTTGACATTG GAGAACCGTT CTGAGCTAAC ACGTATCATT 
GAAGCTATAA CAGACCAATG TCTTCACAAT GAGAAGTTTT TGCTCTATTC GTCGGGAACT 
TTCCCCACGA AGGGGGGCGA TATTGCCAGT CCCGACGAGG TTACTCTGAT TAAGTCAGGA 
TTCGTGAAGA CTACAAAAAT TGTGGATCGG GACGGTGTAC CAGATGCAAT TATGACCGTT 
GACACAACAA AATCGCCATT CTACAAAGAT ACGTCTCTCC TCAAATTTTT CACTGCCAAA 
ATGGATCAAC TTACAAATAG TGGTGGAGGA CCCAGAGGCC ACAATGGTGG CAGAGAGCGT 
CGCGATGGCG GCGGGAACAG TAGAAAATAC GATGATAGAC GCTCACCGCG TGATGGTGAA 
ATTGATTATG ACGAGAGAAC TGTCTCACAT TATCAAAGAC AGTTTCAAGA CGAACGCATT 
TCGGATGGTA TGCTGAACAC TCTCAAGCAG TCTCTCAAAG GATTGGATTG TCAGCCGATT 
CATCTTAAAG ACTCGAAAGC CAATCGTTCA ATCATGATAG ACGAAATTCA CACCGGAACA 
GCAGATAGTG TCACATTTGA ACAAAAACTT CCCGACGGAG AGATGAAGCT CACTTCCATC 
ACAGAGTATT ATTTACAACG GTACAACTAT CGTCTCAAAT TCCCACATCT TCCGTTGGTG 
ACTTCAAAAC GGGCGAAATG TTACGATTTC TATCCTATGG AACTCATGTC CATTCTTCCT 
GGCCAGAGAA TCAAACAGTC ACATATGACT GTTGATATTC AATCTTATAT GACTGGAAAA 
ATGAGCAGCC TGCCTGATCA GCACATCAAA CAGTCGAAAT TGGTTTTGAC GGAATATTTA 
AAACTTGGTG ACCAGCCTGC AAATCGCCAA ATGGATGCTT TCCGTGTGTC GCTCAAAAGC 
ATCCAGCCAA TTGTCACGAA TGCCCATTGG CTCTCCCCAC CAGACATGAA GTTCGCAAAC 
AATCAGCTGT ACAGTCTAAA TCCAACCCGT GGTGTGCGTT TCCAGACTAA TGGCAAGTTT 
GTCATGCCAG CCAGAGTAAA GTCGGTGACG ATTATCAACT ACGACAAGGA ATTTAATAGA 
AACGTCGATA TGTTTGCCGA AGGGCTTGCC AAGCATTGCT CCGAACAAGG AATGAAGTTT 
GATAGCCGCC CGAACAGCTG GAAAAAAGTT AATCTCGGCT CATCAGACCG ACGAGGTACA 
AAAGTGGAGA TAGAAGAAGC CATTCGAAAC GGCGTTACGA TTGTGTTTGG AATTATTGCC 
GAAAAACGAC CAGATATGCA TGACATTCTC AAATACTTTG AGGAGAAGCT TGGACAGCAA 
ACCATTCAAA TTTCTTCTGA AACCGCGGAC AAATTCATGC GTGACCATGG TGGAAAACAA 
ACCATTGACA ATGTCATCAG AAAATTGAAC CCCAAGTGTG GAGGAACTAA CTTCCTAATT 
GACGTGCCCG AGTCGGTTGG CCATCGAGTC GTTTGCAACA ATTCTGCTGA AATGAGAGCA 
AAACTGTACG CAAAGACGCA ATTTATCGGA TTCGAGATGT CGCATACAGG CGCTAGGACC 
AGATTCGACA TTCAGAAAGT TATGTTTGAC GGAGATCCAA CTGTCGTCGG AGTTGCGTAC 
TCTCTCAAAC ACAGTGCTCA GCTTGGAGGG TTTTCATATT TCCAGGAAAG TCGTTTGCAC 
AAGCTCACAA ATCTTCAAGA GAAGATGCAA ATTTGCTTGA ATGCTTACGA GCAATCGAGC 
TCGTATCTTC CTGAGACAGT TGTTGTCTAT CGTGTCGGAA GTGGTGAAGG AGATTATCCG 
CAAATTGTCA ACGAGGTTAA CGAGATGAAG CTGGCTGCAA GAAAGAAGAA GCATGGTTAC 
AATCCCAAGT TTCTCGTTAT TTGCACACAA CGCAACTCTC ACATCCGAGT ATTCCCTGAA 
CACATTAATG AACGTGGAAA GTCCATGGAG CAAAATGTAA AATCCGGCAC TTGCGTCGAT 
GTGCCAGGAG CCTCTCACGG ATACGAGGAG TTCATTCTTT GTTGCCAAAC ACCTCTGATT 
GGAACAGTAA AACCAACAAA GTATACTATC ATTGTCAACG ATTGTCGTTG GAGCAAGAAT 
GAGATCATGA ATGTCACCTA CCACTTGGCG TTTGCCCACC AGGTCTCTTA TGCACCACCA 
GCTATCCCCA ACGTGAGCTA TGCTGCTCAA AATCTTGCTA AACGTGGTCA CAACAACTAC 
AAAACACACA CAAAGCTTGT GGACATGAAC GACTACTCTT ATCGAATCAA GGAAAAACAT 
GAGGAAATCA TTTCGTCAGA GGAAGTTGAC GATATTCTGA TGCGCGATTT CATTGAGACA 
GTTTCCAACG ATCTAAATGC AATGACCATC AACGGACGCA ACTTTTGGGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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nrde-3 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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