rde-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	rde-1
Entrez Gene ID	179393
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_171525.4	NP_741611.1	hypothetical protein
NM_171525.5	NP_741611.1	Piwi domain-containing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results uncouple the role of the RDE-1 RNase H activity in small RNA maturation from its role in target-mRNA silencing in vivo.
Title: RDE-1 slicer activity is required only for passenger-strand cleavage during RNAi in Caenorhabditis elegans.

The majority of Dicer-regulated genes are not dependent on RDE-4 or RDE-1, including the X-linked genes.
Title: Genes misregulated in C. elegans deficient in Dicer, RDE-4, or RDE-1 are enriched for innate immunity genes.

The following rde-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rde-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa354530	NM_171525.5 Latest version!	Caenorhabditis elegans Piwi domain-containing protein (rde-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa354530	NM_171525.4	Caenorhabditis elegans Uncharacterized protein (rde-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rde-1 ( NM_171525.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_741611.1 Caenorhabditis elegans Uncharacterized protein (rde-1), partial mRNA.

Price & Availability↑

CloneID	OCa354530
Clone ID Related Accession (Same CDS sequence)	NM_171525.4 , NM_171525.5
Accession Version	NM_171525.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-09
Organism	Caenorhabditis elegans
Product	hypothetical protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Jun 26, 2012 this sequence version replaced NM_171525.3. COMPLETENESS: incomplete on both ends.

ATGTCCTCGA ATTTTCCCGA ATTGGAAAAA GGATTTTATC GTCATTCTCT CGATCCGGAG 
ATGAAATGGC TTGCGAGGCC CACTGGTAAA TGCGACGGCA AATTCTATGA GAAGAAAGTA 
CTTCTTTTGG TAAATTGGTT CAAGTTCTCC AGCAAAATTT ACGATCGGGA ATACTACGAG 
TATGAAGTGA AAATGACAAA GGAAGTATTG AATAGAAAAC CAGGAAAACC TTTCCCAAAA 
AAGACAGAAA TTCCAATTCC CGATCGTGCA AAACTCTTCT GGCAACATCT TCGGCATGAG 
AAGAAGCAGA CAGATTTTAT TCTCGAAGAC TATGTTTTTG ATGAAAAGGA CACTGTTTAT 
AGTGTTTGTC GACTGAACAC TGTCACATCA AAAATGCTGG TTTCGGAGAA AGTAGTAAAA 
AAGGATTCGG AGAAAAAAGA TGAAAAGGAT TTGGAGAAAA AAATCTTATA CACAATGATA 
CTTACCTATC GTAAAAAATT TCACCTGAAC TTTAGTCGAG AAAATCCGGA AAAAGACGAA 
GAAGCGAATC GGAGTTACAA ATTCCTGAAG AATGTTATGA CCCAGAAAGT TCGCTACGCG 
CCTTTTGTGA ACGAGGAGAT TAAAGTACAA TTCGCGAAAA ATTTTGTGTA CGATAATAAT 
TCAATTCTGC GAGTTCCTGA ATCGTTTCAC GATCCAAACA GATTCGAACA ATCATTAGAA 
GTAGCACCAA GAATCGAAGC ATGGTTTGGA ATTTACATTG GAATCAAAGA ATTGTTCGAT 
GGTGAACCTG TGCTCAATTT TGCAATTGTC GATAAACTAT TCTACAATGC ACCGAAAATG 
TCTCTTCTGG ATTATCTTCT CCTAATTGTC GACCCCCAGT CGTGTAACGA TGATGTACGA 
AAAGATCTTA AAACAAAACT GATGGCGGGA AAAATGACAA TCAGACAAGC CGCGCGGCCA 
AGAATTCGAC AATTATTGGA AAATTTGAAG CTGAAATGCG CAGAAGTTTG GGATAACGAA 
ATGTCGAGAT TGACAGAACG ACATCTGACA TTTCTAGATT TGTGCGAGGA AAACTCTCTT 
GTTTATAAAG TCACTGGTAA ATCGGACAGA GGAAGAAATG CAAAAAAGTA CGATACTACA 
TTGTTCAAAA TCTATGAGGA AAACAAAAAG TTCATTGAGT TTCCCCACCT ACCACTAGTC 
AAAGTTAAAA GTGGAGCAAA AGAATACGCT GTACCAATGG AACATCTTGA AGTTCATGAG 
AAGCCACAAA GATACAAGAA TCGAATTGAT CTGGTGATGC AAGACAAGTT TCTAAAGCGA 
GCTACACGAA AACCTCACGA CTACAAAGAA AATACCCTAA AAATGCTGAA AGAATTGGAT 
TTCTCTTCTG AAGAGCTAAA TTTTGTTGAA AGATTTGGAT TATGCTCCAA ACTTCAGATG 
ATCGAATGTC CAGGAAAGGT TTTGAAAGAG CCAATGCTTG TGAATAGTGT AAATGAACAA 
ATTAAAATGA CACCAGTGAT TCGTGGATTT CAAGAAAAAC AATTGAATGT GGTTCCCGAA 
AAAGAACTTT GCTGTGCTGT TTTTGTAGTC AACGAAACAG CGGGAAATCC ATGCTTAGAA 
GAGAACGACG TTGTTAAGTT CTACACCGAA CTAATTGGTG GTTGCAAGTT CCGTGGAATA 
CGAATTGGTG CCAATGAAAA CAGAGGAGCG CAATCTATTA TGTACGACGC GACGAAAAAT 
GAATATGCCT TCTACAAAAA TTGTACACTA AATACCGGAA TCGGTAGATT TGAAATAGCC 
GCAACAGAAG CGAAGAATAT GTTTGAACGT CTTCCCGATA AAGAACAAAA AGTCTTAATG 
TTCATTATCA TTTCCAAACG ACAACTGAAT GCTTACGGTT TTGTGAAACA TTATTGCGAT 
CACACCATCG GTGTAGCTAA TCAGCATATT ACTTCTGAAA CAGTCACAAA AGCTTTGGCA 
TCACTAAGGC ACGAGAAAGG ATCAAAACGA ATTTTCTATC AAATTGCATT GAAAATCAAC 
GCGAAATTAG GAGGTATTAA CCAGGAGCTT GACTGGTCAG AAATTGCAGA AATATCACCA 
GAAGAAAAAG AAAGACGGAA AACAATGCCA TTAACTATGT ATGTTGGAAT TGATGTAACT 
CATCCAACCT CCTACAGTGG AATTGATTAT TCTATAGCGG CTGTAGTAGC GAGTATCAAT 
CCAGGTGGAA CTATCTATCG AAATATGATT GTGACTCAAG AAGAATGTCG TCCCGGTGAG 
CGTGCAGTGG CTCATGGACG GGAAAGAACA GATATTTTGG AAGCAAAGTT CGTGAAATTG 
CTCAGAGAAT TCGCAGAAAA CAACGACAAT CGAGCACCAG CGCATATTGT AGTCTATCGA 
GACGGAGTTA GCGATTCGGA GATGCTACGT GTTAGTCATG ATGAGCTTCG ATCTTTAAAA 
AGCGAAGTAA AACAATTCAT GTCGGAACGG GATGGAGAAG ATCCAGAGCC GAAGTACACG 
TTCATTGTGA TTCAGAAAAG ACACAATACA CGATTGCTTC GAAGAATGGA AAAAGATAAG 
CCAGTGGTCA ATAAAGATCT TACTCCTGCT GAAACAGATG TCGCTGTTGC TGCTGTTAAA 
CAATGGGAGG AGGATATGAA AGAAAGCAAA GAAACTGGAA TTGTGAACCC ATCATCCGGA 
ACAACTGTGG ATAAACTTAT CGTTTCGAAA TACAAATTCG ATTTTTTCTT GGCATCTCAT 
CATGGTGTCC TTGGTACATC TCGTCCAGGA CATTACACTG TTATGTATGA CGATAAAGGA 
ATGAGCCAAG ATGAAGTCTA TAAAATGACC TACGGACTTG CTTTTCTCTC TGCTAGATGT 
CGAAAACCCA TCTCGTTGCC TGTTCCGGTT CATTATGCTC ATTTATCATG TGAAAAAGCG 
AAAGAGCTTT ATCGAACTTA CAAGGAACAT TACATCGGTG ACTATGCACA GCCACGGACT 
CGACACGAAA TGGAACATTT TCTCCAAACT AACGTGAAGT ACCCTGGAAT GTCGTTCGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_741611.1
CDS	1..3063
Translation	MSSNFPELEKGFYRHSLDPEMKWLARPTGKCDGKFYEKKVLLLVNWFKFSSKIYDREYYEYEVKMTKEVLNRKPGKPFPKKTEIPIPDRAKLFWQHLRHEKKQTDFILEDYVFDEKDTVYSVCRLNTVTSKMLVSEKVVKKDSEKKDEKDLEKKILYTMILTYRKKFHLNFSRENPEKDEEANRSYKFLKNVMTQKVRYAPFVNEEIKVQFAKNFVYDNNSILRVPESFHDPNRFEQSLEVAPRIEAWFGIYIGIKELFDGEPVLNFAIVDKLFYNAPKMSLLDYLLLIVDPQSCNDDVRKDLKTKLMAGKMTIRQAARPRIRQLLENLKLKCAEVWDNEMSRLTERHLTFLDLCEENSLVYKVTGKSDRGRNAKKYDTTLFKIYEENKKFIEFPHLPLVKVKSGAKEYAVPMEHLEVHEKPQRYKNRIDLVMQDKFLKRATRKPHDYKENTLKMLKELDFSSEELNFVERFGLCSKLQMIECPGKVLKEPMLVNSVNEQIKMTPVIRGFQEKQLNVVPEKELCCAVFVVNETAGNPCLEENDVVKFYTELIGGCKFRGIRIGANENRGAQSIMYDATKNEYAFYKNCTLNTGIGRFEIAATEAKNMFERLPDKEQKVLMFIIISKRQLNAYGFVKHYCDHTIGVANQHITSETVTKALASLRHEKGSKRIFYQIALKINAKLGGINQELDWSEIAEISPEEKERRKTMPLTMYVGIDVTHPTSYSGIDYSIAAVVASINPGGTIYRNMIVTQEECRPGERAVAHGRERTDILEAKFVKLLREFAENNDNRAPAHIVVYRDGVSDSEMLRVSHDELRSLKSEVKQFMSERDGEDPEPKYTFIVIQKRHNTRLLRRMEKDKPVVNKDLTPAETDVAVAAVKQWEEDMKESKETGIVNPSSGTTVDKLIVSKYKFDFFLASHHGVLGTSRPGHYTVMYDDKGMSQDEVYKMTYGLAFLSARCRKPISLPVPVHYAHLSCEKAKELYRTYKEHYIGDYAQPRTRHEMEHFLQTNVKYPGMSFA

Target ORF information:

RefSeq Version	NM_171525.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (rde-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_171525.4

ORF Insert Sequence:

ATGTCCTCGA ATTTTCCCGA ATTGGAAAAA GGATTTTATC GTCATTCTCT CGATCCGGAG 
ATGAAATGGC TTGCGAGGCC CACTGGTAAA TGCGACGGCA AATTCTATGA GAAGAAAGTA 
CTTCTTTTGG TAAATTGGTT CAAGTTCTCC AGCAAAATTT ACGATCGGGA ATACTACGAG 
TATGAAGTGA AAATGACAAA GGAAGTATTG AATAGAAAAC CAGGAAAACC TTTCCCAAAA 
AAGACAGAAA TTCCAATTCC CGATCGTGCA AAACTCTTCT GGCAACATCT TCGGCATGAG 
AAGAAGCAGA CAGATTTTAT TCTCGAAGAC TATGTTTTTG ATGAAAAGGA CACTGTTTAT 
AGTGTTTGTC GACTGAACAC TGTCACATCA AAAATGCTGG TTTCGGAGAA AGTAGTAAAA 
AAGGATTCGG AGAAAAAAGA TGAAAAGGAT TTGGAGAAAA AAATCTTATA CACAATGATA 
CTTACCTATC GTAAAAAATT TCACCTGAAC TTTAGTCGAG AAAATCCGGA AAAAGACGAA 
GAAGCGAATC GGAGTTACAA ATTCCTGAAG AATGTTATGA CCCAGAAAGT TCGCTACGCG 
CCTTTTGTGA ACGAGGAGAT TAAAGTACAA TTCGCGAAAA ATTTTGTGTA CGATAATAAT 
TCAATTCTGC GAGTTCCTGA ATCGTTTCAC GATCCAAACA GATTCGAACA ATCATTAGAA 
GTAGCACCAA GAATCGAAGC ATGGTTTGGA ATTTACATTG GAATCAAAGA ATTGTTCGAT 
GGTGAACCTG TGCTCAATTT TGCAATTGTC GATAAACTAT TCTACAATGC ACCGAAAATG 
TCTCTTCTGG ATTATCTTCT CCTAATTGTC GACCCCCAGT CGTGTAACGA TGATGTACGA 
AAAGATCTTA AAACAAAACT GATGGCGGGA AAAATGACAA TCAGACAAGC CGCGCGGCCA 
AGAATTCGAC AATTATTGGA AAATTTGAAG CTGAAATGCG CAGAAGTTTG GGATAACGAA 
ATGTCGAGAT TGACAGAACG ACATCTGACA TTTCTAGATT TGTGCGAGGA AAACTCTCTT 
GTTTATAAAG TCACTGGTAA ATCGGACAGA GGAAGAAATG CAAAAAAGTA CGATACTACA 
TTGTTCAAAA TCTATGAGGA AAACAAAAAG TTCATTGAGT TTCCCCACCT ACCACTAGTC 
AAAGTTAAAA GTGGAGCAAA AGAATACGCT GTACCAATGG AACATCTTGA AGTTCATGAG 
AAGCCACAAA GATACAAGAA TCGAATTGAT CTGGTGATGC AAGACAAGTT TCTAAAGCGA 
GCTACACGAA AACCTCACGA CTACAAAGAA AATACCCTAA AAATGCTGAA AGAATTGGAT 
TTCTCTTCTG AAGAGCTAAA TTTTGTTGAA AGATTTGGAT TATGCTCCAA ACTTCAGATG 
ATCGAATGTC CAGGAAAGGT TTTGAAAGAG CCAATGCTTG TGAATAGTGT AAATGAACAA 
ATTAAAATGA CACCAGTGAT TCGTGGATTT CAAGAAAAAC AATTGAATGT GGTTCCCGAA 
AAAGAACTTT GCTGTGCTGT TTTTGTAGTC AACGAAACAG CGGGAAATCC ATGCTTAGAA 
GAGAACGACG TTGTTAAGTT CTACACCGAA CTAATTGGTG GTTGCAAGTT CCGTGGAATA 
CGAATTGGTG CCAATGAAAA CAGAGGAGCG CAATCTATTA TGTACGACGC GACGAAAAAT 
GAATATGCCT TCTACAAAAA TTGTACACTA AATACCGGAA TCGGTAGATT TGAAATAGCC 
GCAACAGAAG CGAAGAATAT GTTTGAACGT CTTCCCGATA AAGAACAAAA AGTCTTAATG 
TTCATTATCA TTTCCAAACG ACAACTGAAT GCTTACGGTT TTGTGAAACA TTATTGCGAT 
CACACCATCG GTGTAGCTAA TCAGCATATT ACTTCTGAAA CAGTCACAAA AGCTTTGGCA 
TCACTAAGGC ACGAGAAAGG ATCAAAACGA ATTTTCTATC AAATTGCATT GAAAATCAAC 
GCGAAATTAG GAGGTATTAA CCAGGAGCTT GACTGGTCAG AAATTGCAGA AATATCACCA 
GAAGAAAAAG AAAGACGGAA AACAATGCCA TTAACTATGT ATGTTGGAAT TGATGTAACT 
CATCCAACCT CCTACAGTGG AATTGATTAT TCTATAGCGG CTGTAGTAGC GAGTATCAAT 
CCAGGTGGAA CTATCTATCG AAATATGATT GTGACTCAAG AAGAATGTCG TCCCGGTGAG 
CGTGCAGTGG CTCATGGACG GGAAAGAACA GATATTTTGG AAGCAAAGTT CGTGAAATTG 
CTCAGAGAAT TCGCAGAAAA CAACGACAAT CGAGCACCAG CGCATATTGT AGTCTATCGA 
GACGGAGTTA GCGATTCGGA GATGCTACGT GTTAGTCATG ATGAGCTTCG ATCTTTAAAA 
AGCGAAGTAA AACAATTCAT GTCGGAACGG GATGGAGAAG ATCCAGAGCC GAAGTACACG 
TTCATTGTGA TTCAGAAAAG ACACAATACA CGATTGCTTC GAAGAATGGA AAAAGATAAG 
CCAGTGGTCA ATAAAGATCT TACTCCTGCT GAAACAGATG TCGCTGTTGC TGCTGTTAAA 
CAATGGGAGG AGGATATGAA AGAAAGCAAA GAAACTGGAA TTGTGAACCC ATCATCCGGA 
ACAACTGTGG ATAAACTTAT CGTTTCGAAA TACAAATTCG ATTTTTTCTT GGCATCTCAT 
CATGGTGTCC TTGGTACATC TCGTCCAGGA CATTACACTG TTATGTATGA CGATAAAGGA 
ATGAGCCAAG ATGAAGTCTA TAAAATGACC TACGGACTTG CTTTTCTCTC TGCTAGATGT 
CGAAAACCCA TCTCGTTGCC TGTTCCGGTT CATTATGCTC ATTTATCATG TGAAAAAGCG 
AAAGAGCTTT ATCGAACTTA CAAGGAACAT TACATCGGTG ACTATGCACA GCCACGGACT 
CGACACGAAA TGGAACATTT TCTCCAAACT AACGTGAAGT ACCCTGGAAT GTCGTTCGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rde-1 ( NM_171525.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_741611.1 Caenorhabditis elegans Piwi domain-containing protein (rde-1), mRNA.

Price & Availability↑

CloneID	OCa354530
Clone ID Related Accession (Same CDS sequence)	NM_171525.4 , NM_171525.5
Accession Version	NM_171525.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Piwi domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_171525.4.

ATGTCCTCGA ATTTTCCCGA ATTGGAAAAA GGATTTTATC GTCATTCTCT CGATCCGGAG 
ATGAAATGGC TTGCGAGGCC CACTGGTAAA TGCGACGGCA AATTCTATGA GAAGAAAGTA 
CTTCTTTTGG TAAATTGGTT CAAGTTCTCC AGCAAAATTT ACGATCGGGA ATACTACGAG 
TATGAAGTGA AAATGACAAA GGAAGTATTG AATAGAAAAC CAGGAAAACC TTTCCCAAAA 
AAGACAGAAA TTCCAATTCC CGATCGTGCA AAACTCTTCT GGCAACATCT TCGGCATGAG 
AAGAAGCAGA CAGATTTTAT TCTCGAAGAC TATGTTTTTG ATGAAAAGGA CACTGTTTAT 
AGTGTTTGTC GACTGAACAC TGTCACATCA AAAATGCTGG TTTCGGAGAA AGTAGTAAAA 
AAGGATTCGG AGAAAAAAGA TGAAAAGGAT TTGGAGAAAA AAATCTTATA CACAATGATA 
CTTACCTATC GTAAAAAATT TCACCTGAAC TTTAGTCGAG AAAATCCGGA AAAAGACGAA 
GAAGCGAATC GGAGTTACAA ATTCCTGAAG AATGTTATGA CCCAGAAAGT TCGCTACGCG 
CCTTTTGTGA ACGAGGAGAT TAAAGTACAA TTCGCGAAAA ATTTTGTGTA CGATAATAAT 
TCAATTCTGC GAGTTCCTGA ATCGTTTCAC GATCCAAACA GATTCGAACA ATCATTAGAA 
GTAGCACCAA GAATCGAAGC ATGGTTTGGA ATTTACATTG GAATCAAAGA ATTGTTCGAT 
GGTGAACCTG TGCTCAATTT TGCAATTGTC GATAAACTAT TCTACAATGC ACCGAAAATG 
TCTCTTCTGG ATTATCTTCT CCTAATTGTC GACCCCCAGT CGTGTAACGA TGATGTACGA 
AAAGATCTTA AAACAAAACT GATGGCGGGA AAAATGACAA TCAGACAAGC CGCGCGGCCA 
AGAATTCGAC AATTATTGGA AAATTTGAAG CTGAAATGCG CAGAAGTTTG GGATAACGAA 
ATGTCGAGAT TGACAGAACG ACATCTGACA TTTCTAGATT TGTGCGAGGA AAACTCTCTT 
GTTTATAAAG TCACTGGTAA ATCGGACAGA GGAAGAAATG CAAAAAAGTA CGATACTACA 
TTGTTCAAAA TCTATGAGGA AAACAAAAAG TTCATTGAGT TTCCCCACCT ACCACTAGTC 
AAAGTTAAAA GTGGAGCAAA AGAATACGCT GTACCAATGG AACATCTTGA AGTTCATGAG 
AAGCCACAAA GATACAAGAA TCGAATTGAT CTGGTGATGC AAGACAAGTT TCTAAAGCGA 
GCTACACGAA AACCTCACGA CTACAAAGAA AATACCCTAA AAATGCTGAA AGAATTGGAT 
TTCTCTTCTG AAGAGCTAAA TTTTGTTGAA AGATTTGGAT TATGCTCCAA ACTTCAGATG 
ATCGAATGTC CAGGAAAGGT TTTGAAAGAG CCAATGCTTG TGAATAGTGT AAATGAACAA 
ATTAAAATGA CACCAGTGAT TCGTGGATTT CAAGAAAAAC AATTGAATGT GGTTCCCGAA 
AAAGAACTTT GCTGTGCTGT TTTTGTAGTC AACGAAACAG CGGGAAATCC ATGCTTAGAA 
GAGAACGACG TTGTTAAGTT CTACACCGAA CTAATTGGTG GTTGCAAGTT CCGTGGAATA 
CGAATTGGTG CCAATGAAAA CAGAGGAGCG CAATCTATTA TGTACGACGC GACGAAAAAT 
GAATATGCCT TCTACAAAAA TTGTACACTA AATACCGGAA TCGGTAGATT TGAAATAGCC 
GCAACAGAAG CGAAGAATAT GTTTGAACGT CTTCCCGATA AAGAACAAAA AGTCTTAATG 
TTCATTATCA TTTCCAAACG ACAACTGAAT GCTTACGGTT TTGTGAAACA TTATTGCGAT 
CACACCATCG GTGTAGCTAA TCAGCATATT ACTTCTGAAA CAGTCACAAA AGCTTTGGCA 
TCACTAAGGC ACGAGAAAGG ATCAAAACGA ATTTTCTATC AAATTGCATT GAAAATCAAC 
GCGAAATTAG GAGGTATTAA CCAGGAGCTT GACTGGTCAG AAATTGCAGA AATATCACCA 
GAAGAAAAAG AAAGACGGAA AACAATGCCA TTAACTATGT ATGTTGGAAT TGATGTAACT 
CATCCAACCT CCTACAGTGG AATTGATTAT TCTATAGCGG CTGTAGTAGC GAGTATCAAT 
CCAGGTGGAA CTATCTATCG AAATATGATT GTGACTCAAG AAGAATGTCG TCCCGGTGAG 
CGTGCAGTGG CTCATGGACG GGAAAGAACA GATATTTTGG AAGCAAAGTT CGTGAAATTG 
CTCAGAGAAT TCGCAGAAAA CAACGACAAT CGAGCACCAG CGCATATTGT AGTCTATCGA 
GACGGAGTTA GCGATTCGGA GATGCTACGT GTTAGTCATG ATGAGCTTCG ATCTTTAAAA 
AGCGAAGTAA AACAATTCAT GTCGGAACGG GATGGAGAAG ATCCAGAGCC GAAGTACACG 
TTCATTGTGA TTCAGAAAAG ACACAATACA CGATTGCTTC GAAGAATGGA AAAAGATAAG 
CCAGTGGTCA ATAAAGATCT TACTCCTGCT GAAACAGATG TCGCTGTTGC TGCTGTTAAA 
CAATGGGAGG AGGATATGAA AGAAAGCAAA GAAACTGGAA TTGTGAACCC ATCATCCGGA 
ACAACTGTGG ATAAACTTAT CGTTTCGAAA TACAAATTCG ATTTTTTCTT GGCATCTCAT 
CATGGTGTCC TTGGTACATC TCGTCCAGGA CATTACACTG TTATGTATGA CGATAAAGGA 
ATGAGCCAAG ATGAAGTCTA TAAAATGACC TACGGACTTG CTTTTCTCTC TGCTAGATGT 
CGAAAACCCA TCTCGTTGCC TGTTCCGGTT CATTATGCTC ATTTATCATG TGAAAAAGCG 
AAAGAGCTTT ATCGAACTTA CAAGGAACAT TACATCGGTG ACTATGCACA GCCACGGACT 
CGACACGAAA TGGAACATTT TCTCCAAACT AACGTGAAGT ACCCTGGAAT GTCGTTCGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_741611.1
CDS	29..3091
Translation	MSSNFPELEKGFYRHSLDPEMKWLARPTGKCDGKFYEKKVLLLVNWFKFSSKIYDREYYEYEVKMTKEVLNRKPGKPFPKKTEIPIPDRAKLFWQHLRHEKKQTDFILEDYVFDEKDTVYSVCRLNTVTSKMLVSEKVVKKDSEKKDEKDLEKKILYTMILTYRKKFHLNFSRENPEKDEEANRSYKFLKNVMTQKVRYAPFVNEEIKVQFAKNFVYDNNSILRVPESFHDPNRFEQSLEVAPRIEAWFGIYIGIKELFDGEPVLNFAIVDKLFYNAPKMSLLDYLLLIVDPQSCNDDVRKDLKTKLMAGKMTIRQAARPRIRQLLENLKLKCAEVWDNEMSRLTERHLTFLDLCEENSLVYKVTGKSDRGRNAKKYDTTLFKIYEENKKFIEFPHLPLVKVKSGAKEYAVPMEHLEVHEKPQRYKNRIDLVMQDKFLKRATRKPHDYKENTLKMLKELDFSSEELNFVERFGLCSKLQMIECPGKVLKEPMLVNSVNEQIKMTPVIRGFQEKQLNVVPEKELCCAVFVVNETAGNPCLEENDVVKFYTELIGGCKFRGIRIGANENRGAQSIMYDATKNEYAFYKNCTLNTGIGRFEIAATEAKNMFERLPDKEQKVLMFIIISKRQLNAYGFVKHYCDHTIGVANQHITSETVTKALASLRHEKGSKRIFYQIALKINAKLGGINQELDWSEIAEISPEEKERRKTMPLTMYVGIDVTHPTSYSGIDYSIAAVVASINPGGTIYRNMIVTQEECRPGERAVAHGRERTDILEAKFVKLLREFAENNDNRAPAHIVVYRDGVSDSEMLRVSHDELRSLKSEVKQFMSERDGEDPEPKYTFIVIQKRHNTRLLRRMEKDKPVVNKDLTPAETDVAVAAVKQWEEDMKESKETGIVNPSSGTTVDKLIVSKYKFDFFLASHHGVLGTSRPGHYTVMYDDKGMSQDEVYKMTYGLAFLSARCRKPISLPVPVHYAHLSCEKAKELYRTYKEHYIGDYAQPRTRHEMEHFLQTNVKYPGMSFA

Target ORF information:

RefSeq Version	NM_171525.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Piwi domain-containing protein (rde-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_171525.5

ORF Insert Sequence:

ATGTCCTCGA ATTTTCCCGA ATTGGAAAAA GGATTTTATC GTCATTCTCT CGATCCGGAG 
ATGAAATGGC TTGCGAGGCC CACTGGTAAA TGCGACGGCA AATTCTATGA GAAGAAAGTA 
CTTCTTTTGG TAAATTGGTT CAAGTTCTCC AGCAAAATTT ACGATCGGGA ATACTACGAG 
TATGAAGTGA AAATGACAAA GGAAGTATTG AATAGAAAAC CAGGAAAACC TTTCCCAAAA 
AAGACAGAAA TTCCAATTCC CGATCGTGCA AAACTCTTCT GGCAACATCT TCGGCATGAG 
AAGAAGCAGA CAGATTTTAT TCTCGAAGAC TATGTTTTTG ATGAAAAGGA CACTGTTTAT 
AGTGTTTGTC GACTGAACAC TGTCACATCA AAAATGCTGG TTTCGGAGAA AGTAGTAAAA 
AAGGATTCGG AGAAAAAAGA TGAAAAGGAT TTGGAGAAAA AAATCTTATA CACAATGATA 
CTTACCTATC GTAAAAAATT TCACCTGAAC TTTAGTCGAG AAAATCCGGA AAAAGACGAA 
GAAGCGAATC GGAGTTACAA ATTCCTGAAG AATGTTATGA CCCAGAAAGT TCGCTACGCG 
CCTTTTGTGA ACGAGGAGAT TAAAGTACAA TTCGCGAAAA ATTTTGTGTA CGATAATAAT 
TCAATTCTGC GAGTTCCTGA ATCGTTTCAC GATCCAAACA GATTCGAACA ATCATTAGAA 
GTAGCACCAA GAATCGAAGC ATGGTTTGGA ATTTACATTG GAATCAAAGA ATTGTTCGAT 
GGTGAACCTG TGCTCAATTT TGCAATTGTC GATAAACTAT TCTACAATGC ACCGAAAATG 
TCTCTTCTGG ATTATCTTCT CCTAATTGTC GACCCCCAGT CGTGTAACGA TGATGTACGA 
AAAGATCTTA AAACAAAACT GATGGCGGGA AAAATGACAA TCAGACAAGC CGCGCGGCCA 
AGAATTCGAC AATTATTGGA AAATTTGAAG CTGAAATGCG CAGAAGTTTG GGATAACGAA 
ATGTCGAGAT TGACAGAACG ACATCTGACA TTTCTAGATT TGTGCGAGGA AAACTCTCTT 
GTTTATAAAG TCACTGGTAA ATCGGACAGA GGAAGAAATG CAAAAAAGTA CGATACTACA 
TTGTTCAAAA TCTATGAGGA AAACAAAAAG TTCATTGAGT TTCCCCACCT ACCACTAGTC 
AAAGTTAAAA GTGGAGCAAA AGAATACGCT GTACCAATGG AACATCTTGA AGTTCATGAG 
AAGCCACAAA GATACAAGAA TCGAATTGAT CTGGTGATGC AAGACAAGTT TCTAAAGCGA 
GCTACACGAA AACCTCACGA CTACAAAGAA AATACCCTAA AAATGCTGAA AGAATTGGAT 
TTCTCTTCTG AAGAGCTAAA TTTTGTTGAA AGATTTGGAT TATGCTCCAA ACTTCAGATG 
ATCGAATGTC CAGGAAAGGT TTTGAAAGAG CCAATGCTTG TGAATAGTGT AAATGAACAA 
ATTAAAATGA CACCAGTGAT TCGTGGATTT CAAGAAAAAC AATTGAATGT GGTTCCCGAA 
AAAGAACTTT GCTGTGCTGT TTTTGTAGTC AACGAAACAG CGGGAAATCC ATGCTTAGAA 
GAGAACGACG TTGTTAAGTT CTACACCGAA CTAATTGGTG GTTGCAAGTT CCGTGGAATA 
CGAATTGGTG CCAATGAAAA CAGAGGAGCG CAATCTATTA TGTACGACGC GACGAAAAAT 
GAATATGCCT TCTACAAAAA TTGTACACTA AATACCGGAA TCGGTAGATT TGAAATAGCC 
GCAACAGAAG CGAAGAATAT GTTTGAACGT CTTCCCGATA AAGAACAAAA AGTCTTAATG 
TTCATTATCA TTTCCAAACG ACAACTGAAT GCTTACGGTT TTGTGAAACA TTATTGCGAT 
CACACCATCG GTGTAGCTAA TCAGCATATT ACTTCTGAAA CAGTCACAAA AGCTTTGGCA 
TCACTAAGGC ACGAGAAAGG ATCAAAACGA ATTTTCTATC AAATTGCATT GAAAATCAAC 
GCGAAATTAG GAGGTATTAA CCAGGAGCTT GACTGGTCAG AAATTGCAGA AATATCACCA 
GAAGAAAAAG AAAGACGGAA AACAATGCCA TTAACTATGT ATGTTGGAAT TGATGTAACT 
CATCCAACCT CCTACAGTGG AATTGATTAT TCTATAGCGG CTGTAGTAGC GAGTATCAAT 
CCAGGTGGAA CTATCTATCG AAATATGATT GTGACTCAAG AAGAATGTCG TCCCGGTGAG 
CGTGCAGTGG CTCATGGACG GGAAAGAACA GATATTTTGG AAGCAAAGTT CGTGAAATTG 
CTCAGAGAAT TCGCAGAAAA CAACGACAAT CGAGCACCAG CGCATATTGT AGTCTATCGA 
GACGGAGTTA GCGATTCGGA GATGCTACGT GTTAGTCATG ATGAGCTTCG ATCTTTAAAA 
AGCGAAGTAA AACAATTCAT GTCGGAACGG GATGGAGAAG ATCCAGAGCC GAAGTACACG 
TTCATTGTGA TTCAGAAAAG ACACAATACA CGATTGCTTC GAAGAATGGA AAAAGATAAG 
CCAGTGGTCA ATAAAGATCT TACTCCTGCT GAAACAGATG TCGCTGTTGC TGCTGTTAAA 
CAATGGGAGG AGGATATGAA AGAAAGCAAA GAAACTGGAA TTGTGAACCC ATCATCCGGA 
ACAACTGTGG ATAAACTTAT CGTTTCGAAA TACAAATTCG ATTTTTTCTT GGCATCTCAT 
CATGGTGTCC TTGGTACATC TCGTCCAGGA CATTACACTG TTATGTATGA CGATAAAGGA 
ATGAGCCAAG ATGAAGTCTA TAAAATGACC TACGGACTTG CTTTTCTCTC TGCTAGATGT 
CGAAAACCCA TCTCGTTGCC TGTTCCGGTT CATTATGCTC ATTTATCATG TGAAAAAGCG 
AAAGAGCTTT ATCGAACTTA CAAGGAACAT TACATCGGTG ACTATGCACA GCCACGGACT 
CGACACGAAA TGGAACATTT TCTCCAAACT AACGTGAAGT ACCCTGGAAT GTCGTTCGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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rde-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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