ani-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	ani-1
Entrez Gene ID	176672
Full Name	Anillin-like protein 1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_067223.6	NP_499624.2	Anillin-like protein 1
NM_067223.7	NP_499624.2	Anillin-like protein 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

active form of RhoG/MIG-2 directly binds to Anillin and recruits it to the leading edge of Q neuroblasts
Title: Anillin Regulates Neuronal Migration and Neurite Growth by Linking RhoG to the Actin Cytoskeleton.

Caenorhabditis elegans anillin (ani-1) regulates neuroblast cytokinesis and epidermal morphogenesis during embryonic development.
Title: Caenorhabditis elegans anillin (ani-1) regulates neuroblast cytokinesis and epidermal morphogenesis during embryonic development.

Anillin-1 promotes astral microtubule-directed cortical myosin polarization
Title: Anillin promotes astral microtubule-directed cortical myosin polarization.

Anillin is dispensable for contractile ring assembly, initiation, and closure but is required for the meiotic contractile ring to transform from a disc into a tube.
Title: Actomyosin tube formation in polar body cytokinesis requires Anillin in C. elegans.

Asymmetric ingression requires Anillin and the septins, which promote the coalescence of components on one side of the contractile ring, but is insensitive to a 10-fold reduction in myosin II levels.
Title: Anillin and the septins promote asymmetric ingression of the cytokinetic furrow.

The following ani-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ani-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340227	NM_067223.7 Latest version!	Caenorhabditis elegans Anillin-like protein 1 (ani-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa340227	NM_067223.6	Caenorhabditis elegans Anillin-like protein 1 (ani-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ani-1 ( NM_067223.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_499624.2 Caenorhabditis elegans Anillin-like protein 1 (ani-1), partial mRNA.

Price & Availability↑

CloneID	OCa340227
Clone ID Related Accession (Same CDS sequence)	NM_067223.6 , NM_067223.7
Accession Version	NM_067223.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Anillin-like protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_067223.5. COMPLETENESS: incomplete on both ends.

ATGGGGGATC AATTCGATTC TCTAATGGAA CGCATACGAG TTCGGCAAGC CGAAATGTCA 
GGCGAAGGAG AGGTGGCAAA GGAGAATGGG CCAGTGACCT CAAAAATTAC ATCTGTGAAG 
CAAGAAGTTG CATCTCCAAC AAAAGTCTTC GGAAGCTCCT CAAAATGCAA CGACGGCCCG 
TCAACTCCAG TGCATTTTCA CCCTCAAGAG CCAAAGGAGA CAACTCCAAA TATGAAGGAA 
AACGCTGAAA ATTCATTAAA TTCGTTCAAA GATGCCACAG TCAACGAGTC AAGCTCCAAA 
AAGACGTCGA GATTCTCAAT GTTGGCTCAA GAAATCGATG AATACGAATA TGACTACCAA 
TCACAGTACA ATAAGCCGAA AGAAGCATAT ATGAAGGGAA GATCTCCACG AATGTCGATC 
GGAGAGACAC GTCCAGCAGT CCTGTGTACT CCGGCTGGAG CACAAGCCAT GAAAAGCCCA 
AATGTAGCGG TATCGTCGAA AAGTGCTCCC GAAATCGCTC TCGGAATGTC GGATTTCGAA 
GCTCGCCGCA TCAAATTCGC CCAGCCAATT GTGAATGTGA ACTATCTGCC CAACGAATCG 
TCGATTTTCT CCGGAGGATC CGGATCATCT GTAAACGATC AGAGCACTGT GCTCGGAGGC 
TCTGCCGAAA TGATGAACGT CACCACGTCG TCGCTGAGCT GTGGAGAGCT GTCGATGAAT 
CAGCATATAA CACACGAAAA TACGATTATC AACGCACAAT CGTGTGATAA TCGAGAGGCG 
ATTTTGAGAG AAAGACAGCA AGTTTCAAAC GAAGAATATG GACCGCACAC ATTTATGAGA 
AAGAAGGTGC CAAAAGAAGC TTCATCTGCG ACATCATCAT CATCGTCAAC GACAACTTTA 
ACTACGATCT CCGGTGCATC AGGCTCTACA ACGTCTGGAA TATCGAATGC TCCACAGGAC 
TCGGCTTCAA CAAAAACCAC GACTAATACA TTTACTTCTT CGTATTTGCT CACTAAAACC 
TCGAACAACA ACATCAATGC ACTTGTCTCG TCGTCGCCAA AACCGTTTTC TAAAGATATC 
GGAAGATCCA TGTTCTCGCC TGTGCATTTT ACTCCAAAAA GCACGTCGTC ACCGAAAACT 
CTGTCGGAGA GCATCTTCTC GCCATCGAAA TCCGCCGCAG TCGAAGGATC AATTGCGACT 
ACGAGACGGC TTCAGTTTGA GGAGAAATTG AAGAAAAGTA GTAGCGCGAA TGTTACAGCG 
CCTCCTGCAC CTACATCAGC ACCAGTTCCA ACGCCACGCC ACGTGGCTCC ATTGGCACCT 
ACAGTAGCCC AGCAGAGCCA CTTGACTCCA AACCATCGTC ATGCTGCTCA ACAAAAGAAG 
CACCTGTTCC CCGTAGTGGG GATCGTCGCG ACTGCACCGA TTCCAGTTCA AACTCAATGG 
AGAGGACAAT CGAATACTCC AGTTGTGCAG GGAGCTCGTG CCGACGAGAA GACTGCTGGT 
AATGAGCCAC CAGTTGGAGC CGGAGTTGGA AAGCTGAAGA ATTTGAAGTC GAGATGGGAG 
TTTTCGTCGG CAACTGGCAC GCCAATACAT CCTGATGCTA CCGAAGATTC GTTGATTGCT 
ACGGCAATTA AGATGAAGGA GAGTGCGATT CCGAAACAGC TTGGTCACAG ATCAGAGCGA 
AAAGGACCAT CCGCCTCGTC GCTCTACTCG CAAGGTGCTC GGAGTAACAC GGCTTCTCCT 
GCATCGAAAA GTACTCGTTA TGAGCAAGAA GAAGAAGATG ATGTGTTTGA AGCTCCAGAA 
TTTAATGATG GAGGAGACGT GATATCAGAA GATGGAATTC TTCAGGAAGA AGAAGAGGAT 
ACTTCTAAAT TCATTGATAA TGCATTTGGA TTTATGGAAG GATCTGGGGC TGGAACGCCG 
AGCCCATATC GCGAGCCTCC GTTGCAACGT TTGGAAAAGA ATAGGCCGCC GGCGGAGGTT 
ATTGAAGAAG AAACTGAAAA TGAGGATGAA TCTGAACCCT ATGAGCCGGA AGAAGAAGAG 
GATGACGATG CAACAACACA ATTTCCTGTA CCTGAAAGAT CACGAAAATC AAGTTCTCAA 
CTGGCCTACT CTGTTTCGTT TTATCGAAAA ATTCAACGAG ACAGAAACGA AGAATCGTCG 
ACAGTGCTGG CCGGGCCAGT TATCAGTCAA ATCAGTCCAT CTGCTCCGCC GATGTCATCA 
TCGCTCACAT CACAACAGAA ACTTCGACAG CTCACCACCG GGCCTGCGAA TGGAGCACGA 
ATTGTCGAGT CGGCTAAGGA TGCTCACGAT CGTATCAAGC GAGCGATTCA AGTTGAAGAG 
CAGCTTGTTG CTCAATCCAA GAGAGCAATG ATATTGGCCC GGGATAAGCC AAGTTTCCGT 
GGATCGCGTG AGGAATTTGA AGCACAATGG GCAATGCTTC GTCATGTGGA AAAGCATCGA 
GCACTTCTCA CTGAGTATGA TCGATTGAAA CGAGATGGAC CGAGAATTAT CGATGGACCA 
CGTGGAACGA TAACAGTATC CCAGTTGTCA GTGAACATGG CTCGTGATTA TGTGTCAGCT 
AATATTGCGT CAAGCAAGAA ATCCGATGAA GTCTTCTATT TCGCGGCGAT TCTCCGCTAC 
GGCGAGCAAG TCGACGTGTC GAAAATGGTG ACAAGCGACG GTGGTCTGAA TCGTCGTGGT 
GTTCTCGAGT TTCCCGTACC ACTGATGTTG ACCGGGATTC CACCGGATTT CCGAGCAACT 
GTCGAGATTT ATGGACAACG ATCGATGCGA GAATCGACGT CGCACGAGGA TAAGTACAAG 
TTGAAGAATT CGACGTTTAA GGCGAAGACT CGTAACACAT TCCTGGGCGG CGGATCAACT 
TCATCAGCAA ACCAGAGCCT ATTCGTCGAT CCAGCAGCAT CGTCATCATC AACTTCGTCG 
ACCACATCCA ACTTCAATTT GCTCGGAACA TTCTCGTTCG ACATCAATTG CCCTGGAAAA 
CATTTGTACA ACATGTCACA TACTGTATAT CCGCTCGAAG GTATTACACA GATGAAAGTA 
CGCAAACAGG CGATCGATGG AGCTGATATC ACTTATCACG GATTCTTGTC AATGTATCAG 
AGAACTGGCG AGGGGCTCGG ATCGTGGACG AGATACTGGT GTGCTCTTGA GAATGGAGAG 
ATGAAGTTTT GGAAGCAGCC AGAAGATGAG GGAACTAAGG GTTACACTGC ACTAATGGAT 
CTTTCAACGT GTTGCCGTTC CGAAGGTGCA TCGGTAGTTG AAGACATTTG CCCATTCCCG 
AATAGCTTCC ACATTGATGT TTGGGCTCCA AAAATGGATA CATCGGATCC ACGTGGCATT 
GAGCGTCTTC GAGTTATGCT GGCGGCCGAT ACAGCTCAAG ATCTTCAAAC ATGGCTGTCA 
CTGATCAATT CTACGTCGAA GCAGTTGTGC ACGTGGAGAA ATCCGATTGT TAATCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_499624.2
CDS	1..3480
Translation	MGDQFDSLMERIRVRQAEMSGEGEVAKENGPVTSKITSVKQEVASPTKVFGSSSKCNDGPSTPVHFHPQEPKETTPNMKENAENSLNSFKDATVNESSSKKTSRFSMLAQEIDEYEYDYQSQYNKPKEAYMKGRSPRMSIGETRPAVLCTPAGAQAMKSPNVAVSSKSAPEIALGMSDFEARRIKFAQPIVNVNYLPNESSIFSGGSGSSVNDQSTVLGGSAEMMNVTTSSLSCGELSMNQHITHENTIINAQSCDNREAILRERQQVSNEEYGPHTFMRKKVPKEASSATSSSSSTTTLTTISGASGSTTSGISNAPQDSASTKTTTNTFTSSYLLTKTSNNNINALVSSSPKPFSKDIGRSMFSPVHFTPKSTSSPKTLSESIFSPSKSAAVEGSIATTRRLQFEEKLKKSSSANVTAPPAPTSAPVPTPRHVAPLAPTVAQQSHLTPNHRHAAQQKKHLFPVVGIVATAPIPVQTQWRGQSNTPVVQGARADEKTAGNEPPVGAGVGKLKNLKSRWEFSSATGTPIHPDATEDSLIATAIKMKESAIPKQLGHRSERKGPSASSLYSQGARSNTASPASKSTRYEQEEEDDVFEAPEFNDGGDVISEDGILQEEEEDTSKFIDNAFGFMEGSGAGTPSPYREPPLQRLEKNRPPAEVIEEETENEDESEPYEPEEEEDDDATTQFPVPERSRKSSSQLAYSVSFYRKIQRDRNEESSTVLAGPVISQISPSAPPMSSSLTSQQKLRQLTTGPANGARIVESAKDAHDRIKRAIQVEEQLVAQSKRAMILARDKPSFRGSREEFEAQWAMLRHVEKHRALLTEYDRLKRDGPRIIDGPRGTITVSQLSVNMARDYVSANIASSKKSDEVFYFAAILRYGEQVDVSKMVTSDGGLNRRGVLEFPVPLMLTGIPPDFRATVEIYGQRSMRESTSHEDKYKLKNSTFKAKTRNTFLGGGSTSSANQSLFVDPAASSSSTSSTTSNFNLLGTFSFDINCPGKHLYNMSHTVYPLEGITQMKVRKQAIDGADITYHGFLSMYQRTGEGLGSWTRYWCALENGEMKFWKQPEDEGTKGYTALMDLSTCCRSEGASVVEDICPFPNSFHIDVWAPKMDTSDPRGIERLRVMLAADTAQDLQTWLSLINSTSKQLCTWRNPIVNQ

Target ORF information:

RefSeq Version	NM_067223.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Anillin-like protein 1 (ani-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_067223.6

ORF Insert Sequence:

ATGGGGGATC AATTCGATTC TCTAATGGAA CGCATACGAG TTCGGCAAGC CGAAATGTCA 
GGCGAAGGAG AGGTGGCAAA GGAGAATGGG CCAGTGACCT CAAAAATTAC ATCTGTGAAG 
CAAGAAGTTG CATCTCCAAC AAAAGTCTTC GGAAGCTCCT CAAAATGCAA CGACGGCCCG 
TCAACTCCAG TGCATTTTCA CCCTCAAGAG CCAAAGGAGA CAACTCCAAA TATGAAGGAA 
AACGCTGAAA ATTCATTAAA TTCGTTCAAA GATGCCACAG TCAACGAGTC AAGCTCCAAA 
AAGACGTCGA GATTCTCAAT GTTGGCTCAA GAAATCGATG AATACGAATA TGACTACCAA 
TCACAGTACA ATAAGCCGAA AGAAGCATAT ATGAAGGGAA GATCTCCACG AATGTCGATC 
GGAGAGACAC GTCCAGCAGT CCTGTGTACT CCGGCTGGAG CACAAGCCAT GAAAAGCCCA 
AATGTAGCGG TATCGTCGAA AAGTGCTCCC GAAATCGCTC TCGGAATGTC GGATTTCGAA 
GCTCGCCGCA TCAAATTCGC CCAGCCAATT GTGAATGTGA ACTATCTGCC CAACGAATCG 
TCGATTTTCT CCGGAGGATC CGGATCATCT GTAAACGATC AGAGCACTGT GCTCGGAGGC 
TCTGCCGAAA TGATGAACGT CACCACGTCG TCGCTGAGCT GTGGAGAGCT GTCGATGAAT 
CAGCATATAA CACACGAAAA TACGATTATC AACGCACAAT CGTGTGATAA TCGAGAGGCG 
ATTTTGAGAG AAAGACAGCA AGTTTCAAAC GAAGAATATG GACCGCACAC ATTTATGAGA 
AAGAAGGTGC CAAAAGAAGC TTCATCTGCG ACATCATCAT CATCGTCAAC GACAACTTTA 
ACTACGATCT CCGGTGCATC AGGCTCTACA ACGTCTGGAA TATCGAATGC TCCACAGGAC 
TCGGCTTCAA CAAAAACCAC GACTAATACA TTTACTTCTT CGTATTTGCT CACTAAAACC 
TCGAACAACA ACATCAATGC ACTTGTCTCG TCGTCGCCAA AACCGTTTTC TAAAGATATC 
GGAAGATCCA TGTTCTCGCC TGTGCATTTT ACTCCAAAAA GCACGTCGTC ACCGAAAACT 
CTGTCGGAGA GCATCTTCTC GCCATCGAAA TCCGCCGCAG TCGAAGGATC AATTGCGACT 
ACGAGACGGC TTCAGTTTGA GGAGAAATTG AAGAAAAGTA GTAGCGCGAA TGTTACAGCG 
CCTCCTGCAC CTACATCAGC ACCAGTTCCA ACGCCACGCC ACGTGGCTCC ATTGGCACCT 
ACAGTAGCCC AGCAGAGCCA CTTGACTCCA AACCATCGTC ATGCTGCTCA ACAAAAGAAG 
CACCTGTTCC CCGTAGTGGG GATCGTCGCG ACTGCACCGA TTCCAGTTCA AACTCAATGG 
AGAGGACAAT CGAATACTCC AGTTGTGCAG GGAGCTCGTG CCGACGAGAA GACTGCTGGT 
AATGAGCCAC CAGTTGGAGC CGGAGTTGGA AAGCTGAAGA ATTTGAAGTC GAGATGGGAG 
TTTTCGTCGG CAACTGGCAC GCCAATACAT CCTGATGCTA CCGAAGATTC GTTGATTGCT 
ACGGCAATTA AGATGAAGGA GAGTGCGATT CCGAAACAGC TTGGTCACAG ATCAGAGCGA 
AAAGGACCAT CCGCCTCGTC GCTCTACTCG CAAGGTGCTC GGAGTAACAC GGCTTCTCCT 
GCATCGAAAA GTACTCGTTA TGAGCAAGAA GAAGAAGATG ATGTGTTTGA AGCTCCAGAA 
TTTAATGATG GAGGAGACGT GATATCAGAA GATGGAATTC TTCAGGAAGA AGAAGAGGAT 
ACTTCTAAAT TCATTGATAA TGCATTTGGA TTTATGGAAG GATCTGGGGC TGGAACGCCG 
AGCCCATATC GCGAGCCTCC GTTGCAACGT TTGGAAAAGA ATAGGCCGCC GGCGGAGGTT 
ATTGAAGAAG AAACTGAAAA TGAGGATGAA TCTGAACCCT ATGAGCCGGA AGAAGAAGAG 
GATGACGATG CAACAACACA ATTTCCTGTA CCTGAAAGAT CACGAAAATC AAGTTCTCAA 
CTGGCCTACT CTGTTTCGTT TTATCGAAAA ATTCAACGAG ACAGAAACGA AGAATCGTCG 
ACAGTGCTGG CCGGGCCAGT TATCAGTCAA ATCAGTCCAT CTGCTCCGCC GATGTCATCA 
TCGCTCACAT CACAACAGAA ACTTCGACAG CTCACCACCG GGCCTGCGAA TGGAGCACGA 
ATTGTCGAGT CGGCTAAGGA TGCTCACGAT CGTATCAAGC GAGCGATTCA AGTTGAAGAG 
CAGCTTGTTG CTCAATCCAA GAGAGCAATG ATATTGGCCC GGGATAAGCC AAGTTTCCGT 
GGATCGCGTG AGGAATTTGA AGCACAATGG GCAATGCTTC GTCATGTGGA AAAGCATCGA 
GCACTTCTCA CTGAGTATGA TCGATTGAAA CGAGATGGAC CGAGAATTAT CGATGGACCA 
CGTGGAACGA TAACAGTATC CCAGTTGTCA GTGAACATGG CTCGTGATTA TGTGTCAGCT 
AATATTGCGT CAAGCAAGAA ATCCGATGAA GTCTTCTATT TCGCGGCGAT TCTCCGCTAC 
GGCGAGCAAG TCGACGTGTC GAAAATGGTG ACAAGCGACG GTGGTCTGAA TCGTCGTGGT 
GTTCTCGAGT TTCCCGTACC ACTGATGTTG ACCGGGATTC CACCGGATTT CCGAGCAACT 
GTCGAGATTT ATGGACAACG ATCGATGCGA GAATCGACGT CGCACGAGGA TAAGTACAAG 
TTGAAGAATT CGACGTTTAA GGCGAAGACT CGTAACACAT TCCTGGGCGG CGGATCAACT 
TCATCAGCAA ACCAGAGCCT ATTCGTCGAT CCAGCAGCAT CGTCATCATC AACTTCGTCG 
ACCACATCCA ACTTCAATTT GCTCGGAACA TTCTCGTTCG ACATCAATTG CCCTGGAAAA 
CATTTGTACA ACATGTCACA TACTGTATAT CCGCTCGAAG GTATTACACA GATGAAAGTA 
CGCAAACAGG CGATCGATGG AGCTGATATC ACTTATCACG GATTCTTGTC AATGTATCAG 
AGAACTGGCG AGGGGCTCGG ATCGTGGACG AGATACTGGT GTGCTCTTGA GAATGGAGAG 
ATGAAGTTTT GGAAGCAGCC AGAAGATGAG GGAACTAAGG GTTACACTGC ACTAATGGAT 
CTTTCAACGT GTTGCCGTTC CGAAGGTGCA TCGGTAGTTG AAGACATTTG CCCATTCCCG 
AATAGCTTCC ACATTGATGT TTGGGCTCCA AAAATGGATA CATCGGATCC ACGTGGCATT 
GAGCGTCTTC GAGTTATGCT GGCGGCCGAT ACAGCTCAAG ATCTTCAAAC ATGGCTGTCA 
CTGATCAATT CTACGTCGAA GCAGTTGTGC ACGTGGAGAA ATCCGATTGT TAATCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ani-1 ( NM_067223.7 ) cDNA ORF clone, Caenorhabditis elegans -> NP_499624.2 Caenorhabditis elegans Anillin-like protein 1 (ani-1), mRNA.

Price & Availability↑

CloneID	OCa340227
Clone ID Related Accession (Same CDS sequence)	NM_067223.6 , NM_067223.7
Accession Version	NM_067223.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Anillin-like protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_067223.6.

ATGGGGGATC AATTCGATTC TCTAATGGAA CGCATACGAG TTCGGCAAGC CGAAATGTCA 
GGCGAAGGAG AGGTGGCAAA GGAGAATGGG CCAGTGACCT CAAAAATTAC ATCTGTGAAG 
CAAGAAGTTG CATCTCCAAC AAAAGTCTTC GGAAGCTCCT CAAAATGCAA CGACGGCCCG 
TCAACTCCAG TGCATTTTCA CCCTCAAGAG CCAAAGGAGA CAACTCCAAA TATGAAGGAA 
AACGCTGAAA ATTCATTAAA TTCGTTCAAA GATGCCACAG TCAACGAGTC AAGCTCCAAA 
AAGACGTCGA GATTCTCAAT GTTGGCTCAA GAAATCGATG AATACGAATA TGACTACCAA 
TCACAGTACA ATAAGCCGAA AGAAGCATAT ATGAAGGGAA GATCTCCACG AATGTCGATC 
GGAGAGACAC GTCCAGCAGT CCTGTGTACT CCGGCTGGAG CACAAGCCAT GAAAAGCCCA 
AATGTAGCGG TATCGTCGAA AAGTGCTCCC GAAATCGCTC TCGGAATGTC GGATTTCGAA 
GCTCGCCGCA TCAAATTCGC CCAGCCAATT GTGAATGTGA ACTATCTGCC CAACGAATCG 
TCGATTTTCT CCGGAGGATC CGGATCATCT GTAAACGATC AGAGCACTGT GCTCGGAGGC 
TCTGCCGAAA TGATGAACGT CACCACGTCG TCGCTGAGCT GTGGAGAGCT GTCGATGAAT 
CAGCATATAA CACACGAAAA TACGATTATC AACGCACAAT CGTGTGATAA TCGAGAGGCG 
ATTTTGAGAG AAAGACAGCA AGTTTCAAAC GAAGAATATG GACCGCACAC ATTTATGAGA 
AAGAAGGTGC CAAAAGAAGC TTCATCTGCG ACATCATCAT CATCGTCAAC GACAACTTTA 
ACTACGATCT CCGGTGCATC AGGCTCTACA ACGTCTGGAA TATCGAATGC TCCACAGGAC 
TCGGCTTCAA CAAAAACCAC GACTAATACA TTTACTTCTT CGTATTTGCT CACTAAAACC 
TCGAACAACA ACATCAATGC ACTTGTCTCG TCGTCGCCAA AACCGTTTTC TAAAGATATC 
GGAAGATCCA TGTTCTCGCC TGTGCATTTT ACTCCAAAAA GCACGTCGTC ACCGAAAACT 
CTGTCGGAGA GCATCTTCTC GCCATCGAAA TCCGCCGCAG TCGAAGGATC AATTGCGACT 
ACGAGACGGC TTCAGTTTGA GGAGAAATTG AAGAAAAGTA GTAGCGCGAA TGTTACAGCG 
CCTCCTGCAC CTACATCAGC ACCAGTTCCA ACGCCACGCC ACGTGGCTCC ATTGGCACCT 
ACAGTAGCCC AGCAGAGCCA CTTGACTCCA AACCATCGTC ATGCTGCTCA ACAAAAGAAG 
CACCTGTTCC CCGTAGTGGG GATCGTCGCG ACTGCACCGA TTCCAGTTCA AACTCAATGG 
AGAGGACAAT CGAATACTCC AGTTGTGCAG GGAGCTCGTG CCGACGAGAA GACTGCTGGT 
AATGAGCCAC CAGTTGGAGC CGGAGTTGGA AAGCTGAAGA ATTTGAAGTC GAGATGGGAG 
TTTTCGTCGG CAACTGGCAC GCCAATACAT CCTGATGCTA CCGAAGATTC GTTGATTGCT 
ACGGCAATTA AGATGAAGGA GAGTGCGATT CCGAAACAGC TTGGTCACAG ATCAGAGCGA 
AAAGGACCAT CCGCCTCGTC GCTCTACTCG CAAGGTGCTC GGAGTAACAC GGCTTCTCCT 
GCATCGAAAA GTACTCGTTA TGAGCAAGAA GAAGAAGATG ATGTGTTTGA AGCTCCAGAA 
TTTAATGATG GAGGAGACGT GATATCAGAA GATGGAATTC TTCAGGAAGA AGAAGAGGAT 
ACTTCTAAAT TCATTGATAA TGCATTTGGA TTTATGGAAG GATCTGGGGC TGGAACGCCG 
AGCCCATATC GCGAGCCTCC GTTGCAACGT TTGGAAAAGA ATAGGCCGCC GGCGGAGGTT 
ATTGAAGAAG AAACTGAAAA TGAGGATGAA TCTGAACCCT ATGAGCCGGA AGAAGAAGAG 
GATGACGATG CAACAACACA ATTTCCTGTA CCTGAAAGAT CACGAAAATC AAGTTCTCAA 
CTGGCCTACT CTGTTTCGTT TTATCGAAAA ATTCAACGAG ACAGAAACGA AGAATCGTCG 
ACAGTGCTGG CCGGGCCAGT TATCAGTCAA ATCAGTCCAT CTGCTCCGCC GATGTCATCA 
TCGCTCACAT CACAACAGAA ACTTCGACAG CTCACCACCG GGCCTGCGAA TGGAGCACGA 
ATTGTCGAGT CGGCTAAGGA TGCTCACGAT CGTATCAAGC GAGCGATTCA AGTTGAAGAG 
CAGCTTGTTG CTCAATCCAA GAGAGCAATG ATATTGGCCC GGGATAAGCC AAGTTTCCGT 
GGATCGCGTG AGGAATTTGA AGCACAATGG GCAATGCTTC GTCATGTGGA AAAGCATCGA 
GCACTTCTCA CTGAGTATGA TCGATTGAAA CGAGATGGAC CGAGAATTAT CGATGGACCA 
CGTGGAACGA TAACAGTATC CCAGTTGTCA GTGAACATGG CTCGTGATTA TGTGTCAGCT 
AATATTGCGT CAAGCAAGAA ATCCGATGAA GTCTTCTATT TCGCGGCGAT TCTCCGCTAC 
GGCGAGCAAG TCGACGTGTC GAAAATGGTG ACAAGCGACG GTGGTCTGAA TCGTCGTGGT 
GTTCTCGAGT TTCCCGTACC ACTGATGTTG ACCGGGATTC CACCGGATTT CCGAGCAACT 
GTCGAGATTT ATGGACAACG ATCGATGCGA GAATCGACGT CGCACGAGGA TAAGTACAAG 
TTGAAGAATT CGACGTTTAA GGCGAAGACT CGTAACACAT TCCTGGGCGG CGGATCAACT 
TCATCAGCAA ACCAGAGCCT ATTCGTCGAT CCAGCAGCAT CGTCATCATC AACTTCGTCG 
ACCACATCCA ACTTCAATTT GCTCGGAACA TTCTCGTTCG ACATCAATTG CCCTGGAAAA 
CATTTGTACA ACATGTCACA TACTGTATAT CCGCTCGAAG GTATTACACA GATGAAAGTA 
CGCAAACAGG CGATCGATGG AGCTGATATC ACTTATCACG GATTCTTGTC AATGTATCAG 
AGAACTGGCG AGGGGCTCGG ATCGTGGACG AGATACTGGT GTGCTCTTGA GAATGGAGAG 
ATGAAGTTTT GGAAGCAGCC AGAAGATGAG GGAACTAAGG GTTACACTGC ACTAATGGAT 
CTTTCAACGT GTTGCCGTTC CGAAGGTGCA TCGGTAGTTG AAGACATTTG CCCATTCCCG 
AATAGCTTCC ACATTGATGT TTGGGCTCCA AAAATGGATA CATCGGATCC ACGTGGCATT 
GAGCGTCTTC GAGTTATGCT GGCGGCCGAT ACAGCTCAAG ATCTTCAAAC ATGGCTGTCA 
CTGATCAATT CTACGTCGAA GCAGTTGTGC ACGTGGAGAA ATCCGATTGT TAATCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_499624.2
CDS	19..3498
Translation	MGDQFDSLMERIRVRQAEMSGEGEVAKENGPVTSKITSVKQEVASPTKVFGSSSKCNDGPSTPVHFHPQEPKETTPNMKENAENSLNSFKDATVNESSSKKTSRFSMLAQEIDEYEYDYQSQYNKPKEAYMKGRSPRMSIGETRPAVLCTPAGAQAMKSPNVAVSSKSAPEIALGMSDFEARRIKFAQPIVNVNYLPNESSIFSGGSGSSVNDQSTVLGGSAEMMNVTTSSLSCGELSMNQHITHENTIINAQSCDNREAILRERQQVSNEEYGPHTFMRKKVPKEASSATSSSSSTTTLTTISGASGSTTSGISNAPQDSASTKTTTNTFTSSYLLTKTSNNNINALVSSSPKPFSKDIGRSMFSPVHFTPKSTSSPKTLSESIFSPSKSAAVEGSIATTRRLQFEEKLKKSSSANVTAPPAPTSAPVPTPRHVAPLAPTVAQQSHLTPNHRHAAQQKKHLFPVVGIVATAPIPVQTQWRGQSNTPVVQGARADEKTAGNEPPVGAGVGKLKNLKSRWEFSSATGTPIHPDATEDSLIATAIKMKESAIPKQLGHRSERKGPSASSLYSQGARSNTASPASKSTRYEQEEEDDVFEAPEFNDGGDVISEDGILQEEEEDTSKFIDNAFGFMEGSGAGTPSPYREPPLQRLEKNRPPAEVIEEETENEDESEPYEPEEEEDDDATTQFPVPERSRKSSSQLAYSVSFYRKIQRDRNEESSTVLAGPVISQISPSAPPMSSSLTSQQKLRQLTTGPANGARIVESAKDAHDRIKRAIQVEEQLVAQSKRAMILARDKPSFRGSREEFEAQWAMLRHVEKHRALLTEYDRLKRDGPRIIDGPRGTITVSQLSVNMARDYVSANIASSKKSDEVFYFAAILRYGEQVDVSKMVTSDGGLNRRGVLEFPVPLMLTGIPPDFRATVEIYGQRSMRESTSHEDKYKLKNSTFKAKTRNTFLGGGSTSSANQSLFVDPAASSSSTSSTTSNFNLLGTFSFDINCPGKHLYNMSHTVYPLEGITQMKVRKQAIDGADITYHGFLSMYQRTGEGLGSWTRYWCALENGEMKFWKQPEDEGTKGYTALMDLSTCCRSEGASVVEDICPFPNSFHIDVWAPKMDTSDPRGIERLRVMLAADTAQDLQTWLSLINSTSKQLCTWRNPIVNQ

Target ORF information:

RefSeq Version	NM_067223.7
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Anillin-like protein 1 (ani-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_067223.7

ORF Insert Sequence:

ATGGGGGATC AATTCGATTC TCTAATGGAA CGCATACGAG TTCGGCAAGC CGAAATGTCA 
GGCGAAGGAG AGGTGGCAAA GGAGAATGGG CCAGTGACCT CAAAAATTAC ATCTGTGAAG 
CAAGAAGTTG CATCTCCAAC AAAAGTCTTC GGAAGCTCCT CAAAATGCAA CGACGGCCCG 
TCAACTCCAG TGCATTTTCA CCCTCAAGAG CCAAAGGAGA CAACTCCAAA TATGAAGGAA 
AACGCTGAAA ATTCATTAAA TTCGTTCAAA GATGCCACAG TCAACGAGTC AAGCTCCAAA 
AAGACGTCGA GATTCTCAAT GTTGGCTCAA GAAATCGATG AATACGAATA TGACTACCAA 
TCACAGTACA ATAAGCCGAA AGAAGCATAT ATGAAGGGAA GATCTCCACG AATGTCGATC 
GGAGAGACAC GTCCAGCAGT CCTGTGTACT CCGGCTGGAG CACAAGCCAT GAAAAGCCCA 
AATGTAGCGG TATCGTCGAA AAGTGCTCCC GAAATCGCTC TCGGAATGTC GGATTTCGAA 
GCTCGCCGCA TCAAATTCGC CCAGCCAATT GTGAATGTGA ACTATCTGCC CAACGAATCG 
TCGATTTTCT CCGGAGGATC CGGATCATCT GTAAACGATC AGAGCACTGT GCTCGGAGGC 
TCTGCCGAAA TGATGAACGT CACCACGTCG TCGCTGAGCT GTGGAGAGCT GTCGATGAAT 
CAGCATATAA CACACGAAAA TACGATTATC AACGCACAAT CGTGTGATAA TCGAGAGGCG 
ATTTTGAGAG AAAGACAGCA AGTTTCAAAC GAAGAATATG GACCGCACAC ATTTATGAGA 
AAGAAGGTGC CAAAAGAAGC TTCATCTGCG ACATCATCAT CATCGTCAAC GACAACTTTA 
ACTACGATCT CCGGTGCATC AGGCTCTACA ACGTCTGGAA TATCGAATGC TCCACAGGAC 
TCGGCTTCAA CAAAAACCAC GACTAATACA TTTACTTCTT CGTATTTGCT CACTAAAACC 
TCGAACAACA ACATCAATGC ACTTGTCTCG TCGTCGCCAA AACCGTTTTC TAAAGATATC 
GGAAGATCCA TGTTCTCGCC TGTGCATTTT ACTCCAAAAA GCACGTCGTC ACCGAAAACT 
CTGTCGGAGA GCATCTTCTC GCCATCGAAA TCCGCCGCAG TCGAAGGATC AATTGCGACT 
ACGAGACGGC TTCAGTTTGA GGAGAAATTG AAGAAAAGTA GTAGCGCGAA TGTTACAGCG 
CCTCCTGCAC CTACATCAGC ACCAGTTCCA ACGCCACGCC ACGTGGCTCC ATTGGCACCT 
ACAGTAGCCC AGCAGAGCCA CTTGACTCCA AACCATCGTC ATGCTGCTCA ACAAAAGAAG 
CACCTGTTCC CCGTAGTGGG GATCGTCGCG ACTGCACCGA TTCCAGTTCA AACTCAATGG 
AGAGGACAAT CGAATACTCC AGTTGTGCAG GGAGCTCGTG CCGACGAGAA GACTGCTGGT 
AATGAGCCAC CAGTTGGAGC CGGAGTTGGA AAGCTGAAGA ATTTGAAGTC GAGATGGGAG 
TTTTCGTCGG CAACTGGCAC GCCAATACAT CCTGATGCTA CCGAAGATTC GTTGATTGCT 
ACGGCAATTA AGATGAAGGA GAGTGCGATT CCGAAACAGC TTGGTCACAG ATCAGAGCGA 
AAAGGACCAT CCGCCTCGTC GCTCTACTCG CAAGGTGCTC GGAGTAACAC GGCTTCTCCT 
GCATCGAAAA GTACTCGTTA TGAGCAAGAA GAAGAAGATG ATGTGTTTGA AGCTCCAGAA 
TTTAATGATG GAGGAGACGT GATATCAGAA GATGGAATTC TTCAGGAAGA AGAAGAGGAT 
ACTTCTAAAT TCATTGATAA TGCATTTGGA TTTATGGAAG GATCTGGGGC TGGAACGCCG 
AGCCCATATC GCGAGCCTCC GTTGCAACGT TTGGAAAAGA ATAGGCCGCC GGCGGAGGTT 
ATTGAAGAAG AAACTGAAAA TGAGGATGAA TCTGAACCCT ATGAGCCGGA AGAAGAAGAG 
GATGACGATG CAACAACACA ATTTCCTGTA CCTGAAAGAT CACGAAAATC AAGTTCTCAA 
CTGGCCTACT CTGTTTCGTT TTATCGAAAA ATTCAACGAG ACAGAAACGA AGAATCGTCG 
ACAGTGCTGG CCGGGCCAGT TATCAGTCAA ATCAGTCCAT CTGCTCCGCC GATGTCATCA 
TCGCTCACAT CACAACAGAA ACTTCGACAG CTCACCACCG GGCCTGCGAA TGGAGCACGA 
ATTGTCGAGT CGGCTAAGGA TGCTCACGAT CGTATCAAGC GAGCGATTCA AGTTGAAGAG 
CAGCTTGTTG CTCAATCCAA GAGAGCAATG ATATTGGCCC GGGATAAGCC AAGTTTCCGT 
GGATCGCGTG AGGAATTTGA AGCACAATGG GCAATGCTTC GTCATGTGGA AAAGCATCGA 
GCACTTCTCA CTGAGTATGA TCGATTGAAA CGAGATGGAC CGAGAATTAT CGATGGACCA 
CGTGGAACGA TAACAGTATC CCAGTTGTCA GTGAACATGG CTCGTGATTA TGTGTCAGCT 
AATATTGCGT CAAGCAAGAA ATCCGATGAA GTCTTCTATT TCGCGGCGAT TCTCCGCTAC 
GGCGAGCAAG TCGACGTGTC GAAAATGGTG ACAAGCGACG GTGGTCTGAA TCGTCGTGGT 
GTTCTCGAGT TTCCCGTACC ACTGATGTTG ACCGGGATTC CACCGGATTT CCGAGCAACT 
GTCGAGATTT ATGGACAACG ATCGATGCGA GAATCGACGT CGCACGAGGA TAAGTACAAG 
TTGAAGAATT CGACGTTTAA GGCGAAGACT CGTAACACAT TCCTGGGCGG CGGATCAACT 
TCATCAGCAA ACCAGAGCCT ATTCGTCGAT CCAGCAGCAT CGTCATCATC AACTTCGTCG 
ACCACATCCA ACTTCAATTT GCTCGGAACA TTCTCGTTCG ACATCAATTG CCCTGGAAAA 
CATTTGTACA ACATGTCACA TACTGTATAT CCGCTCGAAG GTATTACACA GATGAAAGTA 
CGCAAACAGG CGATCGATGG AGCTGATATC ACTTATCACG GATTCTTGTC AATGTATCAG 
AGAACTGGCG AGGGGCTCGG ATCGTGGACG AGATACTGGT GTGCTCTTGA GAATGGAGAG 
ATGAAGTTTT GGAAGCAGCC AGAAGATGAG GGAACTAAGG GTTACACTGC ACTAATGGAT 
CTTTCAACGT GTTGCCGTTC CGAAGGTGCA TCGGTAGTTG AAGACATTTG CCCATTCCCG 
AATAGCTTCC ACATTGATGT TTGGGCTCCA AAAATGGATA CATCGGATCC ACGTGGCATT 
GAGCGTCTTC GAGTTATGCT GGCGGCCGAT ACAGCTCAAG ATCTTCAAAC ATGGCTGTCA 
CTGATCAATT CTACGTCGAA GCAGTTGTGC ACGTGGAGAA ATCCGATTGT TAATCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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ani-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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