nhl-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	nhl-1
Entrez Gene ID	176295
Full Name	NHL (ring finger b-box coiled coil) domain containing
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_066627.5	NP_499028.1	RING finger protein nhl-1
NM_001322726.1	NP_001309582.1	RING finger protein nhl-1
NM_066627.6	NP_499028.1	RING finger protein nhl-1
NM_001322726.2	NP_001309582.1	RING-type domain-containing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NHL-1 acts in chemosensory neurons to promote stress resistance in distal tissues by DAF-16 activation but is dispensable for the activation of HSF-1.
Title: Differential regulation of the heat shock factor 1 and DAF-16 by neuronal nhl-1 in the nematode C. elegans.

The following nhl-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nhl-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340394	NM_001322726.2 Latest version!	Caenorhabditis elegans RING-type domain-containing protein (nhl-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCa341785	NM_066627.6 Latest version!	Caenorhabditis elegans RING finger protein nhl-1 (nhl-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCa341785	NM_066627.5	Caenorhabditis elegans RING finger protein nhl-1 (nhl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa340394	NM_001322726.1	Caenorhabditis elegans RING finger protein nhl-1 (nhl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nhl-1 ( NM_001322726.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001309582.1 Caenorhabditis elegans RING finger protein nhl-1 (nhl-1), partial mRNA.

Price & Availability↑

CloneID	OCa340394
Clone ID Related Accession (Same CDS sequence)	NM_001322726.1 , NM_001322726.2
Accession Version	NM_001322726.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	RING finger protein nhl-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). COMPLETENESS: incomplete on both ends.

ATGAGCAGTT CTCCACAAAA TGAAGCAGAA GCTCGCGAAA AGATGCGCGA GCTGATGTCA 
CGTCCACCAT CGAGTCGACC AGCTGATTTT GTGAATCCTC TCGAAAAGAT CGAGCAGTTG 
CTGACGTGTC CGATTTGTCT CGATCGCTAC AAACAACCCA AGCTTCTACC TTGTCAACAC 
ACATTTTGCT ATCCATGCTT GGAAAGTTGT GCAGACACGT TACATCGCAA TTTGAAATGT 
CCAGAATGTC GAGCAGAACA CAATATTCCA TATGACGGAG TAAAGGCGTT TCAGCCAAAC 
TACACATTAA CCGGGTTTCT CGAGATTCAT CTGCAGGCCA CACCAGAATC GGCTGCAGAA 
ATTGAAGAGT ACATTCACAG GTACAATTTG GAACGGTGCA AGATTTGTGA TGAGAAAGCA 
GACTGTGAAC CATGTGCACA TTGTGATAGA AAAGCATGTA AAGAATGTCG ACAGACTCAT 
ATGGATATGC TTAAAAGAGA CATGTCGCGA CTTTTGAATC AGGTTAAACG GCTTGCCAAT 
CGAATTACGG AAGCATCGGA TAATTTATCA AAAGGAGTTG ATATGATGAC AATGAACTGT 
GAGACAACAA AAGCAGAAAT TAAAGAATAT TTCCACAGAT ACCAGAGAGA TCTGAAAAAG 
AAGGAAGACA ACTTTCTCAT GGAAGTTGAT ACATTTCAAG CTACTGAGAC TCGTAATATG 
AGCAATCTTC GTGATGTTCT GGAAATTGAA AGTTCTAATA TGAGTGAAGC AGTTTCTAGA 
CTGGATGCTG CAATCAAGGG AGAATGTTCA ATTGAAGATT CTGAACTTGT TCGAATGAAA 
AACACGTTTA CGGAAGGGCT TGAATATCTT AGGAACTTTC AGCCCGACGC CGACGAACTT 
TTTAATCGAA AACTCCGATT CTCAGCTGGA GATGATGCTG CTAAGCTTCC AGCAGCGATC 
ACTACTTCTG GTGAACTTTG TGTTCTAGTT CCTCAATTTT CGGGAAGATA CCTCCCTCTT 
GAACAATCAT ACCTTCCAAG ACCATTCCGT TTACCATTGG AAAGTGACAG TTATCGGGTT 
AAATCTGACG AAAGAGCAAG TATGAGAGAT CGAGAAGCTG ATAGGACATC TTCAAGGCAC 
AGTCATCGAA ATCCTGAGCC TGATGAGTCC TCGATTCGTT ACCGTCGTCG TCAGCAGCTG 
GAAGACGAAG CTTGGAATCG TCTCCGTAAT TCATCTGCTG CTCCTTCTCT TCTTACTACA 
TCAGTCACTG CGGACTCTTC ATCACGAACA TCGCCGTGGG CTGCTGATCG TGTCACCCGA 
TCAGTTGAAC CAACCAAGTC ACGGCCAACA AGTCTGATTG TTCCGAATAC TGAAACACCA 
AGAACTGTAT CTCCAGCTAG TAAACCTCCT CTTCCACCAC AATCTGTTGA ACGGGTTGAG 
AGGACTGAAG ATGCATCACC TGCTCCACTT CCACAACTTC CGATCCGAAA GCCACCTCTG 
CCACGTCAAC AGTCCAGTAA CGATGATTCT TTGAATGAGA AAGTGGAAAC CATCCGTAGA 
GCTCATCAAC AACGTCAAGA TGCATCTAGA GCTGCATCAA GAGCAGTTTC AAGTGAAGAA 
TCAGAGGGAG AGGATTTTCC CGTATCCACC AACCGCGGTA GAATACGAAT AGTTTGTCGC 
GCGGCATCTG TAAACCGTGA TGATGGACTG ATGAGTATGA TCCCAGGAAC TGGTACGATC 
ATAAATGTTC CGCCACCTCA ACATCAACTT CCGGCATCCG CTCCGATCAC CAATGGGACA 
TCTGAACAAG TCGCGATTCC AGTCACACAT TTCACAGACT CTTCTACGAC AACCGACGAA 
GAGAAAGAGA AGCTTGCCGT TTTGCGACAG CGTGGCCGCA GCGCTTCTCG TGAAGCTGGT 
GAATGGAGGG CTCGTGGTAG ACCACGTGCA GTATTCGGAC GAAAAGGTGC GAAGGATGGC 
GAGTTAAACT GGCCACGTGG AATTTGTGCA TTGTCCGGAG GTCTTGTCGC AACTTGTGAT 
TCGTCTAATC ATCGTGTCTG TGTTTTCGAT AAAGATGGAA AATTTGTTCG TCAGTTCGGA 
GGCTACGGTG CCGGTGCCGG ACAACTTGAT TCAGCTGCCG GGCTAGCAAG CTCAAAGCTC 
CGAGTTATTG TATCCGACAG GTACAATCAT CGAATTTCCG TTTTTGGATT GGAAGGAGAT 
CATTTGTTCT CATTCGGTGG ACATGGACAG GGAAATGCTA AGTTCAATAA TCCATGGGGA 
GTTGCTGTCG ATGATTTGGG AAGTATCTAT GTGGCTGATA AGGATAATCA CCGCGTCCAA 
GTCTTCGACA AAAATGGTCA ATTCATTGCA AAATTCGGAT CTTTCGGACA TCTACCTGGT 
CAACTAAACA GTCCATTGTT CATAGCAGTT AGCAGAGTTA CTCATCACGT GTATGTATCA 
GATTCTTCGA ATCATCGAAT CAGCGTATTC GATCCCCACG GTGTTCATTT GTTTTCATTC 
GGCGAGGAAG GTTTTCATGG TGGACAATTC AAATTCCCAC GTGGAATTGC TATTGATTCA 
CAGGAAAACC TAATAATCGC CGACTCCGGT AACAACAGGA TCCAAGTTTT CGACGCCCAA 
GGTCAATTTG TCTCCTCTTT CGGAACTTGG GGTGGAGGAG CTGGACAACT GAAAGGTGTC 
GAAGATGTTT GTGTCACTGC TGACGGTTCA ATTGTTGTCA CCGATCGCGA GAATCATCGC 
ATTCAGATCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309582.1
CDS	1..2775
Translation	MSSSPQNEAEAREKMRELMSRPPSSRPADFVNPLEKIEQLLTCPICLDRYKQPKLLPCQHTFCYPCLESCADTLHRNLKCPECRAEHNIPYDGVKAFQPNYTLTGFLEIHLQATPESAAEIEEYIHRYNLERCKICDEKADCEPCAHCDRKACKECRQTHMDMLKRDMSRLLNQVKRLANRITEASDNLSKGVDMMTMNCETTKAEIKEYFHRYQRDLKKKEDNFLMEVDTFQATETRNMSNLRDVLEIESSNMSEAVSRLDAAIKGECSIEDSELVRMKNTFTEGLEYLRNFQPDADELFNRKLRFSAGDDAAKLPAAITTSGELCVLVPQFSGRYLPLEQSYLPRPFRLPLESDSYRVKSDERASMRDREADRTSSRHSHRNPEPDESSIRYRRRQQLEDEAWNRLRNSSAAPSLLTTSVTADSSSRTSPWAADRVTRSVEPTKSRPTSLIVPNTETPRTVSPASKPPLPPQSVERVERTEDASPAPLPQLPIRKPPLPRQQSSNDDSLNEKVETIRRAHQQRQDASRAASRAVSSEESEGEDFPVSTNRGRIRIVCRAASVNRDDGLMSMIPGTGTIINVPPPQHQLPASAPITNGTSEQVAIPVTHFTDSSTTTDEEKEKLAVLRQRGRSASREAGEWRARGRPRAVFGRKGAKDGELNWPRGICALSGGLVATCDSSNHRVCVFDKDGKFVRQFGGYGAGAGQLDSAAGLASSKLRVIVSDRYNHRISVFGLEGDHLFSFGGHGQGNAKFNNPWGVAVDDLGSIYVADKDNHRVQVFDKNGQFIAKFGSFGHLPGQLNSPLFIAVSRVTHHVYVSDSSNHRISVFDPHGVHLFSFGEEGFHGGQFKFPRGIAIDSQENLIIADSGNNRIQVFDAQGQFVSSFGTWGGGAGQLKGVEDVCVTADGSIVVTDRENHRIQIF

Target ORF information:

RefSeq Version	NM_001322726.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans RING finger protein nhl-1 (nhl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322726.1

ORF Insert Sequence:

ATGAGCAGTT CTCCACAAAA TGAAGCAGAA GCTCGCGAAA AGATGCGCGA GCTGATGTCA 
CGTCCACCAT CGAGTCGACC AGCTGATTTT GTGAATCCTC TCGAAAAGAT CGAGCAGTTG 
CTGACGTGTC CGATTTGTCT CGATCGCTAC AAACAACCCA AGCTTCTACC TTGTCAACAC 
ACATTTTGCT ATCCATGCTT GGAAAGTTGT GCAGACACGT TACATCGCAA TTTGAAATGT 
CCAGAATGTC GAGCAGAACA CAATATTCCA TATGACGGAG TAAAGGCGTT TCAGCCAAAC 
TACACATTAA CCGGGTTTCT CGAGATTCAT CTGCAGGCCA CACCAGAATC GGCTGCAGAA 
ATTGAAGAGT ACATTCACAG GTACAATTTG GAACGGTGCA AGATTTGTGA TGAGAAAGCA 
GACTGTGAAC CATGTGCACA TTGTGATAGA AAAGCATGTA AAGAATGTCG ACAGACTCAT 
ATGGATATGC TTAAAAGAGA CATGTCGCGA CTTTTGAATC AGGTTAAACG GCTTGCCAAT 
CGAATTACGG AAGCATCGGA TAATTTATCA AAAGGAGTTG ATATGATGAC AATGAACTGT 
GAGACAACAA AAGCAGAAAT TAAAGAATAT TTCCACAGAT ACCAGAGAGA TCTGAAAAAG 
AAGGAAGACA ACTTTCTCAT GGAAGTTGAT ACATTTCAAG CTACTGAGAC TCGTAATATG 
AGCAATCTTC GTGATGTTCT GGAAATTGAA AGTTCTAATA TGAGTGAAGC AGTTTCTAGA 
CTGGATGCTG CAATCAAGGG AGAATGTTCA ATTGAAGATT CTGAACTTGT TCGAATGAAA 
AACACGTTTA CGGAAGGGCT TGAATATCTT AGGAACTTTC AGCCCGACGC CGACGAACTT 
TTTAATCGAA AACTCCGATT CTCAGCTGGA GATGATGCTG CTAAGCTTCC AGCAGCGATC 
ACTACTTCTG GTGAACTTTG TGTTCTAGTT CCTCAATTTT CGGGAAGATA CCTCCCTCTT 
GAACAATCAT ACCTTCCAAG ACCATTCCGT TTACCATTGG AAAGTGACAG TTATCGGGTT 
AAATCTGACG AAAGAGCAAG TATGAGAGAT CGAGAAGCTG ATAGGACATC TTCAAGGCAC 
AGTCATCGAA ATCCTGAGCC TGATGAGTCC TCGATTCGTT ACCGTCGTCG TCAGCAGCTG 
GAAGACGAAG CTTGGAATCG TCTCCGTAAT TCATCTGCTG CTCCTTCTCT TCTTACTACA 
TCAGTCACTG CGGACTCTTC ATCACGAACA TCGCCGTGGG CTGCTGATCG TGTCACCCGA 
TCAGTTGAAC CAACCAAGTC ACGGCCAACA AGTCTGATTG TTCCGAATAC TGAAACACCA 
AGAACTGTAT CTCCAGCTAG TAAACCTCCT CTTCCACCAC AATCTGTTGA ACGGGTTGAG 
AGGACTGAAG ATGCATCACC TGCTCCACTT CCACAACTTC CGATCCGAAA GCCACCTCTG 
CCACGTCAAC AGTCCAGTAA CGATGATTCT TTGAATGAGA AAGTGGAAAC CATCCGTAGA 
GCTCATCAAC AACGTCAAGA TGCATCTAGA GCTGCATCAA GAGCAGTTTC AAGTGAAGAA 
TCAGAGGGAG AGGATTTTCC CGTATCCACC AACCGCGGTA GAATACGAAT AGTTTGTCGC 
GCGGCATCTG TAAACCGTGA TGATGGACTG ATGAGTATGA TCCCAGGAAC TGGTACGATC 
ATAAATGTTC CGCCACCTCA ACATCAACTT CCGGCATCCG CTCCGATCAC CAATGGGACA 
TCTGAACAAG TCGCGATTCC AGTCACACAT TTCACAGACT CTTCTACGAC AACCGACGAA 
GAGAAAGAGA AGCTTGCCGT TTTGCGACAG CGTGGCCGCA GCGCTTCTCG TGAAGCTGGT 
GAATGGAGGG CTCGTGGTAG ACCACGTGCA GTATTCGGAC GAAAAGGTGC GAAGGATGGC 
GAGTTAAACT GGCCACGTGG AATTTGTGCA TTGTCCGGAG GTCTTGTCGC AACTTGTGAT 
TCGTCTAATC ATCGTGTCTG TGTTTTCGAT AAAGATGGAA AATTTGTTCG TCAGTTCGGA 
GGCTACGGTG CCGGTGCCGG ACAACTTGAT TCAGCTGCCG GGCTAGCAAG CTCAAAGCTC 
CGAGTTATTG TATCCGACAG GTACAATCAT CGAATTTCCG TTTTTGGATT GGAAGGAGAT 
CATTTGTTCT CATTCGGTGG ACATGGACAG GGAAATGCTA AGTTCAATAA TCCATGGGGA 
GTTGCTGTCG ATGATTTGGG AAGTATCTAT GTGGCTGATA AGGATAATCA CCGCGTCCAA 
GTCTTCGACA AAAATGGTCA ATTCATTGCA AAATTCGGAT CTTTCGGACA TCTACCTGGT 
CAACTAAACA GTCCATTGTT CATAGCAGTT AGCAGAGTTA CTCATCACGT GTATGTATCA 
GATTCTTCGA ATCATCGAAT CAGCGTATTC GATCCCCACG GTGTTCATTT GTTTTCATTC 
GGCGAGGAAG GTTTTCATGG TGGACAATTC AAATTCCCAC GTGGAATTGC TATTGATTCA 
CAGGAAAACC TAATAATCGC CGACTCCGGT AACAACAGGA TCCAAGTTTT CGACGCCCAA 
GGTCAATTTG TCTCCTCTTT CGGAACTTGG GGTGGAGGAG CTGGACAACT GAAAGGTGTC 
GAAGATGTTT GTGTCACTGC TGACGGTTCA ATTGTTGTCA CCGATCGCGA GAATCATCGC 
ATTCAGATCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhl-1 ( NM_001322726.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001309582.1 Caenorhabditis elegans RING-type domain-containing protein (nhl-1), mRNA.

Price & Availability↑

CloneID	OCa340394
Clone ID Related Accession (Same CDS sequence)	NM_001322726.1 , NM_001322726.2
Accession Version	NM_001322726.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	RING-type domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001322726.1.

ATGAGCAGTT CTCCACAAAA TGAAGCAGAA GCTCGCGAAA AGATGCGCGA GCTGATGTCA 
CGTCCACCAT CGAGTCGACC AGCTGATTTT GTGAATCCTC TCGAAAAGAT CGAGCAGTTG 
CTGACGTGTC CGATTTGTCT CGATCGCTAC AAACAACCCA AGCTTCTACC TTGTCAACAC 
ACATTTTGCT ATCCATGCTT GGAAAGTTGT GCAGACACGT TACATCGCAA TTTGAAATGT 
CCAGAATGTC GAGCAGAACA CAATATTCCA TATGACGGAG TAAAGGCGTT TCAGCCAAAC 
TACACATTAA CCGGGTTTCT CGAGATTCAT CTGCAGGCCA CACCAGAATC GGCTGCAGAA 
ATTGAAGAGT ACATTCACAG GTACAATTTG GAACGGTGCA AGATTTGTGA TGAGAAAGCA 
GACTGTGAAC CATGTGCACA TTGTGATAGA AAAGCATGTA AAGAATGTCG ACAGACTCAT 
ATGGATATGC TTAAAAGAGA CATGTCGCGA CTTTTGAATC AGGTTAAACG GCTTGCCAAT 
CGAATTACGG AAGCATCGGA TAATTTATCA AAAGGAGTTG ATATGATGAC AATGAACTGT 
GAGACAACAA AAGCAGAAAT TAAAGAATAT TTCCACAGAT ACCAGAGAGA TCTGAAAAAG 
AAGGAAGACA ACTTTCTCAT GGAAGTTGAT ACATTTCAAG CTACTGAGAC TCGTAATATG 
AGCAATCTTC GTGATGTTCT GGAAATTGAA AGTTCTAATA TGAGTGAAGC AGTTTCTAGA 
CTGGATGCTG CAATCAAGGG AGAATGTTCA ATTGAAGATT CTGAACTTGT TCGAATGAAA 
AACACGTTTA CGGAAGGGCT TGAATATCTT AGGAACTTTC AGCCCGACGC CGACGAACTT 
TTTAATCGAA AACTCCGATT CTCAGCTGGA GATGATGCTG CTAAGCTTCC AGCAGCGATC 
ACTACTTCTG GTGAACTTTG TGTTCTAGTT CCTCAATTTT CGGGAAGATA CCTCCCTCTT 
GAACAATCAT ACCTTCCAAG ACCATTCCGT TTACCATTGG AAAGTGACAG TTATCGGGTT 
AAATCTGACG AAAGAGCAAG TATGAGAGAT CGAGAAGCTG ATAGGACATC TTCAAGGCAC 
AGTCATCGAA ATCCTGAGCC TGATGAGTCC TCGATTCGTT ACCGTCGTCG TCAGCAGCTG 
GAAGACGAAG CTTGGAATCG TCTCCGTAAT TCATCTGCTG CTCCTTCTCT TCTTACTACA 
TCAGTCACTG CGGACTCTTC ATCACGAACA TCGCCGTGGG CTGCTGATCG TGTCACCCGA 
TCAGTTGAAC CAACCAAGTC ACGGCCAACA AGTCTGATTG TTCCGAATAC TGAAACACCA 
AGAACTGTAT CTCCAGCTAG TAAACCTCCT CTTCCACCAC AATCTGTTGA ACGGGTTGAG 
AGGACTGAAG ATGCATCACC TGCTCCACTT CCACAACTTC CGATCCGAAA GCCACCTCTG 
CCACGTCAAC AGTCCAGTAA CGATGATTCT TTGAATGAGA AAGTGGAAAC CATCCGTAGA 
GCTCATCAAC AACGTCAAGA TGCATCTAGA GCTGCATCAA GAGCAGTTTC AAGTGAAGAA 
TCAGAGGGAG AGGATTTTCC CGTATCCACC AACCGCGGTA GAATACGAAT AGTTTGTCGC 
GCGGCATCTG TAAACCGTGA TGATGGACTG ATGAGTATGA TCCCAGGAAC TGGTACGATC 
ATAAATGTTC CGCCACCTCA ACATCAACTT CCGGCATCCG CTCCGATCAC CAATGGGACA 
TCTGAACAAG TCGCGATTCC AGTCACACAT TTCACAGACT CTTCTACGAC AACCGACGAA 
GAGAAAGAGA AGCTTGCCGT TTTGCGACAG CGTGGCCGCA GCGCTTCTCG TGAAGCTGGT 
GAATGGAGGG CTCGTGGTAG ACCACGTGCA GTATTCGGAC GAAAAGGTGC GAAGGATGGC 
GAGTTAAACT GGCCACGTGG AATTTGTGCA TTGTCCGGAG GTCTTGTCGC AACTTGTGAT 
TCGTCTAATC ATCGTGTCTG TGTTTTCGAT AAAGATGGAA AATTTGTTCG TCAGTTCGGA 
GGCTACGGTG CCGGTGCCGG ACAACTTGAT TCAGCTGCCG GGCTAGCAAG CTCAAAGCTC 
CGAGTTATTG TATCCGACAG GTACAATCAT CGAATTTCCG TTTTTGGATT GGAAGGAGAT 
CATTTGTTCT CATTCGGTGG ACATGGACAG GGAAATGCTA AGTTCAATAA TCCATGGGGA 
GTTGCTGTCG ATGATTTGGG AAGTATCTAT GTGGCTGATA AGGATAATCA CCGCGTCCAA 
GTCTTCGACA AAAATGGTCA ATTCATTGCA AAATTCGGAT CTTTCGGACA TCTACCTGGT 
CAACTAAACA GTCCATTGTT CATAGCAGTT AGCAGAGTTA CTCATCACGT GTATGTATCA 
GATTCTTCGA ATCATCGAAT CAGCGTATTC GATCCCCACG GTGTTCATTT GTTTTCATTC 
GGCGAGGAAG GTTTTCATGG TGGACAATTC AAATTCCCAC GTGGAATTGC TATTGATTCA 
CAGGAAAACC TAATAATCGC CGACTCCGGT AACAACAGGA TCCAAGTTTT CGACGCCCAA 
GGTCAATTTG TCTCCTCTTT CGGAACTTGG GGTGGAGGAG CTGGACAACT GAAAGGTGTC 
GAAGATGTTT GTGTCACTGC TGACGGTTCA ATTGTTGTCA CCGATCGCGA GAATCATCGC 
ATTCAGATCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309582.1
CDS	106..2880
Translation	MSSSPQNEAEAREKMRELMSRPPSSRPADFVNPLEKIEQLLTCPICLDRYKQPKLLPCQHTFCYPCLESCADTLHRNLKCPECRAEHNIPYDGVKAFQPNYTLTGFLEIHLQATPESAAEIEEYIHRYNLERCKICDEKADCEPCAHCDRKACKECRQTHMDMLKRDMSRLLNQVKRLANRITEASDNLSKGVDMMTMNCETTKAEIKEYFHRYQRDLKKKEDNFLMEVDTFQATETRNMSNLRDVLEIESSNMSEAVSRLDAAIKGECSIEDSELVRMKNTFTEGLEYLRNFQPDADELFNRKLRFSAGDDAAKLPAAITTSGELCVLVPQFSGRYLPLEQSYLPRPFRLPLESDSYRVKSDERASMRDREADRTSSRHSHRNPEPDESSIRYRRRQQLEDEAWNRLRNSSAAPSLLTTSVTADSSSRTSPWAADRVTRSVEPTKSRPTSLIVPNTETPRTVSPASKPPLPPQSVERVERTEDASPAPLPQLPIRKPPLPRQQSSNDDSLNEKVETIRRAHQQRQDASRAASRAVSSEESEGEDFPVSTNRGRIRIVCRAASVNRDDGLMSMIPGTGTIINVPPPQHQLPASAPITNGTSEQVAIPVTHFTDSSTTTDEEKEKLAVLRQRGRSASREAGEWRARGRPRAVFGRKGAKDGELNWPRGICALSGGLVATCDSSNHRVCVFDKDGKFVRQFGGYGAGAGQLDSAAGLASSKLRVIVSDRYNHRISVFGLEGDHLFSFGGHGQGNAKFNNPWGVAVDDLGSIYVADKDNHRVQVFDKNGQFIAKFGSFGHLPGQLNSPLFIAVSRVTHHVYVSDSSNHRISVFDPHGVHLFSFGEEGFHGGQFKFPRGIAIDSQENLIIADSGNNRIQVFDAQGQFVSSFGTWGGGAGQLKGVEDVCVTADGSIVVTDRENHRIQIF

Target ORF information:

RefSeq Version	NM_001322726.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans RING-type domain-containing protein (nhl-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322726.2

ORF Insert Sequence:

ATGAGCAGTT CTCCACAAAA TGAAGCAGAA GCTCGCGAAA AGATGCGCGA GCTGATGTCA 
CGTCCACCAT CGAGTCGACC AGCTGATTTT GTGAATCCTC TCGAAAAGAT CGAGCAGTTG 
CTGACGTGTC CGATTTGTCT CGATCGCTAC AAACAACCCA AGCTTCTACC TTGTCAACAC 
ACATTTTGCT ATCCATGCTT GGAAAGTTGT GCAGACACGT TACATCGCAA TTTGAAATGT 
CCAGAATGTC GAGCAGAACA CAATATTCCA TATGACGGAG TAAAGGCGTT TCAGCCAAAC 
TACACATTAA CCGGGTTTCT CGAGATTCAT CTGCAGGCCA CACCAGAATC GGCTGCAGAA 
ATTGAAGAGT ACATTCACAG GTACAATTTG GAACGGTGCA AGATTTGTGA TGAGAAAGCA 
GACTGTGAAC CATGTGCACA TTGTGATAGA AAAGCATGTA AAGAATGTCG ACAGACTCAT 
ATGGATATGC TTAAAAGAGA CATGTCGCGA CTTTTGAATC AGGTTAAACG GCTTGCCAAT 
CGAATTACGG AAGCATCGGA TAATTTATCA AAAGGAGTTG ATATGATGAC AATGAACTGT 
GAGACAACAA AAGCAGAAAT TAAAGAATAT TTCCACAGAT ACCAGAGAGA TCTGAAAAAG 
AAGGAAGACA ACTTTCTCAT GGAAGTTGAT ACATTTCAAG CTACTGAGAC TCGTAATATG 
AGCAATCTTC GTGATGTTCT GGAAATTGAA AGTTCTAATA TGAGTGAAGC AGTTTCTAGA 
CTGGATGCTG CAATCAAGGG AGAATGTTCA ATTGAAGATT CTGAACTTGT TCGAATGAAA 
AACACGTTTA CGGAAGGGCT TGAATATCTT AGGAACTTTC AGCCCGACGC CGACGAACTT 
TTTAATCGAA AACTCCGATT CTCAGCTGGA GATGATGCTG CTAAGCTTCC AGCAGCGATC 
ACTACTTCTG GTGAACTTTG TGTTCTAGTT CCTCAATTTT CGGGAAGATA CCTCCCTCTT 
GAACAATCAT ACCTTCCAAG ACCATTCCGT TTACCATTGG AAAGTGACAG TTATCGGGTT 
AAATCTGACG AAAGAGCAAG TATGAGAGAT CGAGAAGCTG ATAGGACATC TTCAAGGCAC 
AGTCATCGAA ATCCTGAGCC TGATGAGTCC TCGATTCGTT ACCGTCGTCG TCAGCAGCTG 
GAAGACGAAG CTTGGAATCG TCTCCGTAAT TCATCTGCTG CTCCTTCTCT TCTTACTACA 
TCAGTCACTG CGGACTCTTC ATCACGAACA TCGCCGTGGG CTGCTGATCG TGTCACCCGA 
TCAGTTGAAC CAACCAAGTC ACGGCCAACA AGTCTGATTG TTCCGAATAC TGAAACACCA 
AGAACTGTAT CTCCAGCTAG TAAACCTCCT CTTCCACCAC AATCTGTTGA ACGGGTTGAG 
AGGACTGAAG ATGCATCACC TGCTCCACTT CCACAACTTC CGATCCGAAA GCCACCTCTG 
CCACGTCAAC AGTCCAGTAA CGATGATTCT TTGAATGAGA AAGTGGAAAC CATCCGTAGA 
GCTCATCAAC AACGTCAAGA TGCATCTAGA GCTGCATCAA GAGCAGTTTC AAGTGAAGAA 
TCAGAGGGAG AGGATTTTCC CGTATCCACC AACCGCGGTA GAATACGAAT AGTTTGTCGC 
GCGGCATCTG TAAACCGTGA TGATGGACTG ATGAGTATGA TCCCAGGAAC TGGTACGATC 
ATAAATGTTC CGCCACCTCA ACATCAACTT CCGGCATCCG CTCCGATCAC CAATGGGACA 
TCTGAACAAG TCGCGATTCC AGTCACACAT TTCACAGACT CTTCTACGAC AACCGACGAA 
GAGAAAGAGA AGCTTGCCGT TTTGCGACAG CGTGGCCGCA GCGCTTCTCG TGAAGCTGGT 
GAATGGAGGG CTCGTGGTAG ACCACGTGCA GTATTCGGAC GAAAAGGTGC GAAGGATGGC 
GAGTTAAACT GGCCACGTGG AATTTGTGCA TTGTCCGGAG GTCTTGTCGC AACTTGTGAT 
TCGTCTAATC ATCGTGTCTG TGTTTTCGAT AAAGATGGAA AATTTGTTCG TCAGTTCGGA 
GGCTACGGTG CCGGTGCCGG ACAACTTGAT TCAGCTGCCG GGCTAGCAAG CTCAAAGCTC 
CGAGTTATTG TATCCGACAG GTACAATCAT CGAATTTCCG TTTTTGGATT GGAAGGAGAT 
CATTTGTTCT CATTCGGTGG ACATGGACAG GGAAATGCTA AGTTCAATAA TCCATGGGGA 
GTTGCTGTCG ATGATTTGGG AAGTATCTAT GTGGCTGATA AGGATAATCA CCGCGTCCAA 
GTCTTCGACA AAAATGGTCA ATTCATTGCA AAATTCGGAT CTTTCGGACA TCTACCTGGT 
CAACTAAACA GTCCATTGTT CATAGCAGTT AGCAGAGTTA CTCATCACGT GTATGTATCA 
GATTCTTCGA ATCATCGAAT CAGCGTATTC GATCCCCACG GTGTTCATTT GTTTTCATTC 
GGCGAGGAAG GTTTTCATGG TGGACAATTC AAATTCCCAC GTGGAATTGC TATTGATTCA 
CAGGAAAACC TAATAATCGC CGACTCCGGT AACAACAGGA TCCAAGTTTT CGACGCCCAA 
GGTCAATTTG TCTCCTCTTT CGGAACTTGG GGTGGAGGAG CTGGACAACT GAAAGGTGTC 
GAAGATGTTT GTGTCACTGC TGACGGTTCA ATTGTTGTCA CCGATCGCGA GAATCATCGC 
ATTCAGATCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhl-1 ( NM_066627.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_499028.1 Caenorhabditis elegans RING finger protein nhl-1 (nhl-1), partial mRNA.

Price & Availability↑

CloneID	OCa341785
Clone ID Related Accession (Same CDS sequence)	NM_066627.5 , NM_066627.6
Accession Version	NM_066627.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	RING finger protein nhl-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_066627.4. COMPLETENESS: incomplete on both ends.

ATGAGCAGTT CTCCACAAAA TGAAGCAGAA GCTCGCGAAA AGATGCGCGA GCTGATGTCA 
CGTCCACCAT CGAGTCGACC AGCTGATTTT GTGAATCCTC TCGAAAAGAT CGAGCAGTTG 
CTGACGTGTC CGATTTGTCT CGATCGCTAC AAACAACCCA AGCTTCTACC TTGTCAACAC 
ACATTTTGCT ATCCATGCTT GGAAAGTTGT GCAGACACGT TACATCGCAA TTTGAAATGT 
CCAGAATGTC GAGCAGAACA CAATATTCCA TATGACGGAG TAAAGGCGTT TCAGCCAAAC 
TACACATTAA CCGGGTTTCT CGAGATTCAT CTGCAGGCCA CACCAGAATC GGCTGCAGAA 
ATTGAAGAGT ACATTCACAG GTACAATTTG GAACGGTGCA AGATTTGTGA TGAGAAAGCA 
GACTGTGAAC CATGTGCACA TTGTGATAGA AAAGCATGTA AAGAATGTCG ACAGACTCAT 
ATGGATATGC TTAAAAGAGA CATGTCGCGA CTTTTGAATC AGGTTAAACG GCTTGCCAAT 
CGAATTACGG AAGCATCGGA TAATTTATCA AAAGGAGTTG ATATGATGAC AATGAACTGT 
GAGACAACAA AAGCAGAAAT TAAAGAATAT TTCCACAGAT ACCAGAGAGA TCTGAAAAAG 
AAGGAAGACA ACTTTCTCAT GGAAGTTGAT ACATTTCAAG CTACTGAGAC TCGTAATATG 
AGCAATCTTC GTGATGTTCT GGAAATTGAA AGTTCTAATA TGAGTGAAGC AGTTTCTAGA 
CTGGATGCTG CAATCAAGGG AGAATGTTCA ATTGAAGATT CTGAACTTGT TCGAATGAAA 
AACACGTTTA CGGAAGGGCT TGAATATCTT AGGAACTTTC AGCCCGACGC CGACGAACTT 
TTTAATCGAA AACTCCGATT CTCAGCTGGA GATGATGCTG CTAAGCTTCC AGCAGCGATC 
ACTACTTCTG GTGAACTTTG TGTTCTAGTT CCTCAATTTT CGGGAAGATA CCTCCCTCTT 
GAACAATCAT ACCTTCCAAG ACCATTCCGT TTACCATTGG AAAGTGACAG TTATCGGGTT 
AAATCTGACG AAAGAGCAAG TATGAGAGAT CGAGAAGCTG ATAGGACATC TTCAAGGCAC 
AGTCATCGAA ATCCTGAGCC TGATGAGTCC TCGATTCGTT ACCGTCGTCG TCAGCAGCTG 
GAAGACGAAG CTTGGAATCG TCTCCGTAAT TCATCTGCTG CTCCTTCTCT TCTTACTACA 
TCAGTCACTG CGGACTCTTC ATCACGAACA TCGCCGTGGG CTGCTGATCG TGTCACCCGA 
TCAGTTGAAC CAACCAAGTC ACGGCCAACA AGTCTGATTG TTCCGAATAC TGAAACACCA 
AGAACTGTAT CTCCAGCTAG TAAACCTCCT CTTCCACCAC AATCTGTTGA ACGGGTTGAG 
AGGACTGAAG ATGCATCACC TGCTCCACTT CCACAACTTC CGATCCGAAA GCCACCTCTG 
CCACGTCAAC AGTCCAGTAA CGATGATTCT TTGAATGAGA AAGTGGAAAC CATCCGTAGA 
GCTCATCAAC AACGTCAAGA TGCATCTAGA GCTGCATCAA GAGCAGTTTC AAGTGAAGAA 
TCAGAGGGAG AGGATTTTCC CGTATCCACC AACCGCGGTA GAATACGAAT AGTTTGTCGC 
GCGGCATCTG TAAACCGTGA TGATGGACTG ATGAGTATGA TCCCAGGAAC TGGTACGATC 
ATAAATGTTC CGCCACCTCA ACATCAACTT CCGGCATCCG CTCCGATCAC CAATGGGACA 
TCTGAACAAG TCGCGATTCC AGTCACACAT TTCACAGGTG AGGAAGACGA AAGTGACATC 
GTCGTGCCAG CGTGGTTACA GCGTCGGAGG CAACGCTTCC AACGCTCGCG CACAAATCCC 
GACATACAGG CACAGTTCAC AAGTGCTCGA GTGCAGCAGC TTCTCGCCGA ACGTCAATCT 
CGTTTAGACT CTTCTACGAC AACCGACGAA GAGAAAGAGA AGCTTGCCGT TTTGCGACAG 
CGTGGCCGCA GCGCTTCTCG TGAAGCTGGT GAATGGAGGG CTCGTGGTAG ACCACGTGCA 
GTATTCGGAC GAAAAGGTGC GAAGGATGGC GAGTTAAACT GGCCACGTGG AATTTGTGCA 
TTGTCCGGAG GTCTTGTCGC AACTTGTGAT TCGTCTAATC ATCGTGTCTG TGTTTTCGAT 
AAAGATGGAA AATTTGTTCG TCAGTTCGGA GGCTACGGTG CCGGTGCCGG ACAACTTGAT 
TCAGCTGCCG GGCTAGCAAG CTCAAAGCTC CGAGTTATTG TATCCGACAG GTACAATCAT 
CGAATTTCCG TTTTTGGATT GGAAGGAGAT CATTTGTTCT CATTCGGTGG ACATGGACAG 
GGAAATGCTA AGTTCAATAA TCCATGGGGA GTTGCTGTCG ATGATTTGGG AAGTATCTAT 
GTGGCTGATA AGGATAATCA CCGCGTCCAA GTCTTCGACA AAAATGGTCA ATTCATTGCA 
AAATTCGGAT CTTTCGGACA TCTACCTGGT CAACTAAACA GTCCATTGTT CATAGCAGTT 
AGCAGAGTTA CTCATCACGT GTATGTATCA GATTCTTCGA ATCATCGAAT CAGCGTATTC 
GATCCCCACG GTGTTCATTT GTTTTCATTC GGCGAGGAAG GTTTTCATGG TGGACAATTC 
AAATTCCCAC GTGGAATTGC TATTGATTCA CAGGAAAACC TAATAATCGC CGACTCCGGT 
AACAACAGGA TCCAAGTTTT CGACGCCCAA GGTCAATTTG TCTCCTCTTT CGGAACTTGG 
GGTGGAGGAG CTGGACAACT GAAAGGTGTC GAAGATGTTT GTGTCACTGC TGACGGTTCA 
ATTGTTGTCA CCGATCGCGA GAATCATCGC ATTCAGATCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_499028.1
CDS	1..2925
Translation	MSSSPQNEAEAREKMRELMSRPPSSRPADFVNPLEKIEQLLTCPICLDRYKQPKLLPCQHTFCYPCLESCADTLHRNLKCPECRAEHNIPYDGVKAFQPNYTLTGFLEIHLQATPESAAEIEEYIHRYNLERCKICDEKADCEPCAHCDRKACKECRQTHMDMLKRDMSRLLNQVKRLANRITEASDNLSKGVDMMTMNCETTKAEIKEYFHRYQRDLKKKEDNFLMEVDTFQATETRNMSNLRDVLEIESSNMSEAVSRLDAAIKGECSIEDSELVRMKNTFTEGLEYLRNFQPDADELFNRKLRFSAGDDAAKLPAAITTSGELCVLVPQFSGRYLPLEQSYLPRPFRLPLESDSYRVKSDERASMRDREADRTSSRHSHRNPEPDESSIRYRRRQQLEDEAWNRLRNSSAAPSLLTTSVTADSSSRTSPWAADRVTRSVEPTKSRPTSLIVPNTETPRTVSPASKPPLPPQSVERVERTEDASPAPLPQLPIRKPPLPRQQSSNDDSLNEKVETIRRAHQQRQDASRAASRAVSSEESEGEDFPVSTNRGRIRIVCRAASVNRDDGLMSMIPGTGTIINVPPPQHQLPASAPITNGTSEQVAIPVTHFTGEEDESDIVVPAWLQRRRQRFQRSRTNPDIQAQFTSARVQQLLAERQSRLDSSTTTDEEKEKLAVLRQRGRSASREAGEWRARGRPRAVFGRKGAKDGELNWPRGICALSGGLVATCDSSNHRVCVFDKDGKFVRQFGGYGAGAGQLDSAAGLASSKLRVIVSDRYNHRISVFGLEGDHLFSFGGHGQGNAKFNNPWGVAVDDLGSIYVADKDNHRVQVFDKNGQFIAKFGSFGHLPGQLNSPLFIAVSRVTHHVYVSDSSNHRISVFDPHGVHLFSFGEEGFHGGQFKFPRGIAIDSQENLIIADSGNNRIQVFDAQGQFVSSFGTWGGGAGQLKGVEDVCVTADGSIVVTDRENHRIQIF

Target ORF information:

RefSeq Version	NM_066627.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans RING finger protein nhl-1 (nhl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066627.5

ORF Insert Sequence:

ATGAGCAGTT CTCCACAAAA TGAAGCAGAA GCTCGCGAAA AGATGCGCGA GCTGATGTCA 
CGTCCACCAT CGAGTCGACC AGCTGATTTT GTGAATCCTC TCGAAAAGAT CGAGCAGTTG 
CTGACGTGTC CGATTTGTCT CGATCGCTAC AAACAACCCA AGCTTCTACC TTGTCAACAC 
ACATTTTGCT ATCCATGCTT GGAAAGTTGT GCAGACACGT TACATCGCAA TTTGAAATGT 
CCAGAATGTC GAGCAGAACA CAATATTCCA TATGACGGAG TAAAGGCGTT TCAGCCAAAC 
TACACATTAA CCGGGTTTCT CGAGATTCAT CTGCAGGCCA CACCAGAATC GGCTGCAGAA 
ATTGAAGAGT ACATTCACAG GTACAATTTG GAACGGTGCA AGATTTGTGA TGAGAAAGCA 
GACTGTGAAC CATGTGCACA TTGTGATAGA AAAGCATGTA AAGAATGTCG ACAGACTCAT 
ATGGATATGC TTAAAAGAGA CATGTCGCGA CTTTTGAATC AGGTTAAACG GCTTGCCAAT 
CGAATTACGG AAGCATCGGA TAATTTATCA AAAGGAGTTG ATATGATGAC AATGAACTGT 
GAGACAACAA AAGCAGAAAT TAAAGAATAT TTCCACAGAT ACCAGAGAGA TCTGAAAAAG 
AAGGAAGACA ACTTTCTCAT GGAAGTTGAT ACATTTCAAG CTACTGAGAC TCGTAATATG 
AGCAATCTTC GTGATGTTCT GGAAATTGAA AGTTCTAATA TGAGTGAAGC AGTTTCTAGA 
CTGGATGCTG CAATCAAGGG AGAATGTTCA ATTGAAGATT CTGAACTTGT TCGAATGAAA 
AACACGTTTA CGGAAGGGCT TGAATATCTT AGGAACTTTC AGCCCGACGC CGACGAACTT 
TTTAATCGAA AACTCCGATT CTCAGCTGGA GATGATGCTG CTAAGCTTCC AGCAGCGATC 
ACTACTTCTG GTGAACTTTG TGTTCTAGTT CCTCAATTTT CGGGAAGATA CCTCCCTCTT 
GAACAATCAT ACCTTCCAAG ACCATTCCGT TTACCATTGG AAAGTGACAG TTATCGGGTT 
AAATCTGACG AAAGAGCAAG TATGAGAGAT CGAGAAGCTG ATAGGACATC TTCAAGGCAC 
AGTCATCGAA ATCCTGAGCC TGATGAGTCC TCGATTCGTT ACCGTCGTCG TCAGCAGCTG 
GAAGACGAAG CTTGGAATCG TCTCCGTAAT TCATCTGCTG CTCCTTCTCT TCTTACTACA 
TCAGTCACTG CGGACTCTTC ATCACGAACA TCGCCGTGGG CTGCTGATCG TGTCACCCGA 
TCAGTTGAAC CAACCAAGTC ACGGCCAACA AGTCTGATTG TTCCGAATAC TGAAACACCA 
AGAACTGTAT CTCCAGCTAG TAAACCTCCT CTTCCACCAC AATCTGTTGA ACGGGTTGAG 
AGGACTGAAG ATGCATCACC TGCTCCACTT CCACAACTTC CGATCCGAAA GCCACCTCTG 
CCACGTCAAC AGTCCAGTAA CGATGATTCT TTGAATGAGA AAGTGGAAAC CATCCGTAGA 
GCTCATCAAC AACGTCAAGA TGCATCTAGA GCTGCATCAA GAGCAGTTTC AAGTGAAGAA 
TCAGAGGGAG AGGATTTTCC CGTATCCACC AACCGCGGTA GAATACGAAT AGTTTGTCGC 
GCGGCATCTG TAAACCGTGA TGATGGACTG ATGAGTATGA TCCCAGGAAC TGGTACGATC 
ATAAATGTTC CGCCACCTCA ACATCAACTT CCGGCATCCG CTCCGATCAC CAATGGGACA 
TCTGAACAAG TCGCGATTCC AGTCACACAT TTCACAGGTG AGGAAGACGA AAGTGACATC 
GTCGTGCCAG CGTGGTTACA GCGTCGGAGG CAACGCTTCC AACGCTCGCG CACAAATCCC 
GACATACAGG CACAGTTCAC AAGTGCTCGA GTGCAGCAGC TTCTCGCCGA ACGTCAATCT 
CGTTTAGACT CTTCTACGAC AACCGACGAA GAGAAAGAGA AGCTTGCCGT TTTGCGACAG 
CGTGGCCGCA GCGCTTCTCG TGAAGCTGGT GAATGGAGGG CTCGTGGTAG ACCACGTGCA 
GTATTCGGAC GAAAAGGTGC GAAGGATGGC GAGTTAAACT GGCCACGTGG AATTTGTGCA 
TTGTCCGGAG GTCTTGTCGC AACTTGTGAT TCGTCTAATC ATCGTGTCTG TGTTTTCGAT 
AAAGATGGAA AATTTGTTCG TCAGTTCGGA GGCTACGGTG CCGGTGCCGG ACAACTTGAT 
TCAGCTGCCG GGCTAGCAAG CTCAAAGCTC CGAGTTATTG TATCCGACAG GTACAATCAT 
CGAATTTCCG TTTTTGGATT GGAAGGAGAT CATTTGTTCT CATTCGGTGG ACATGGACAG 
GGAAATGCTA AGTTCAATAA TCCATGGGGA GTTGCTGTCG ATGATTTGGG AAGTATCTAT 
GTGGCTGATA AGGATAATCA CCGCGTCCAA GTCTTCGACA AAAATGGTCA ATTCATTGCA 
AAATTCGGAT CTTTCGGACA TCTACCTGGT CAACTAAACA GTCCATTGTT CATAGCAGTT 
AGCAGAGTTA CTCATCACGT GTATGTATCA GATTCTTCGA ATCATCGAAT CAGCGTATTC 
GATCCCCACG GTGTTCATTT GTTTTCATTC GGCGAGGAAG GTTTTCATGG TGGACAATTC 
AAATTCCCAC GTGGAATTGC TATTGATTCA CAGGAAAACC TAATAATCGC CGACTCCGGT 
AACAACAGGA TCCAAGTTTT CGACGCCCAA GGTCAATTTG TCTCCTCTTT CGGAACTTGG 
GGTGGAGGAG CTGGACAACT GAAAGGTGTC GAAGATGTTT GTGTCACTGC TGACGGTTCA 
ATTGTTGTCA CCGATCGCGA GAATCATCGC ATTCAGATCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhl-1 ( NM_066627.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_499028.1 Caenorhabditis elegans RING finger protein nhl-1 (nhl-1), mRNA.

Price & Availability↑

CloneID	OCa341785
Clone ID Related Accession (Same CDS sequence)	NM_066627.5 , NM_066627.6
Accession Version	NM_066627.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	RING finger protein nhl-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_066627.5.

ATGAGCAGTT CTCCACAAAA TGAAGCAGAA GCTCGCGAAA AGATGCGCGA GCTGATGTCA 
CGTCCACCAT CGAGTCGACC AGCTGATTTT GTGAATCCTC TCGAAAAGAT CGAGCAGTTG 
CTGACGTGTC CGATTTGTCT CGATCGCTAC AAACAACCCA AGCTTCTACC TTGTCAACAC 
ACATTTTGCT ATCCATGCTT GGAAAGTTGT GCAGACACGT TACATCGCAA TTTGAAATGT 
CCAGAATGTC GAGCAGAACA CAATATTCCA TATGACGGAG TAAAGGCGTT TCAGCCAAAC 
TACACATTAA CCGGGTTTCT CGAGATTCAT CTGCAGGCCA CACCAGAATC GGCTGCAGAA 
ATTGAAGAGT ACATTCACAG GTACAATTTG GAACGGTGCA AGATTTGTGA TGAGAAAGCA 
GACTGTGAAC CATGTGCACA TTGTGATAGA AAAGCATGTA AAGAATGTCG ACAGACTCAT 
ATGGATATGC TTAAAAGAGA CATGTCGCGA CTTTTGAATC AGGTTAAACG GCTTGCCAAT 
CGAATTACGG AAGCATCGGA TAATTTATCA AAAGGAGTTG ATATGATGAC AATGAACTGT 
GAGACAACAA AAGCAGAAAT TAAAGAATAT TTCCACAGAT ACCAGAGAGA TCTGAAAAAG 
AAGGAAGACA ACTTTCTCAT GGAAGTTGAT ACATTTCAAG CTACTGAGAC TCGTAATATG 
AGCAATCTTC GTGATGTTCT GGAAATTGAA AGTTCTAATA TGAGTGAAGC AGTTTCTAGA 
CTGGATGCTG CAATCAAGGG AGAATGTTCA ATTGAAGATT CTGAACTTGT TCGAATGAAA 
AACACGTTTA CGGAAGGGCT TGAATATCTT AGGAACTTTC AGCCCGACGC CGACGAACTT 
TTTAATCGAA AACTCCGATT CTCAGCTGGA GATGATGCTG CTAAGCTTCC AGCAGCGATC 
ACTACTTCTG GTGAACTTTG TGTTCTAGTT CCTCAATTTT CGGGAAGATA CCTCCCTCTT 
GAACAATCAT ACCTTCCAAG ACCATTCCGT TTACCATTGG AAAGTGACAG TTATCGGGTT 
AAATCTGACG AAAGAGCAAG TATGAGAGAT CGAGAAGCTG ATAGGACATC TTCAAGGCAC 
AGTCATCGAA ATCCTGAGCC TGATGAGTCC TCGATTCGTT ACCGTCGTCG TCAGCAGCTG 
GAAGACGAAG CTTGGAATCG TCTCCGTAAT TCATCTGCTG CTCCTTCTCT TCTTACTACA 
TCAGTCACTG CGGACTCTTC ATCACGAACA TCGCCGTGGG CTGCTGATCG TGTCACCCGA 
TCAGTTGAAC CAACCAAGTC ACGGCCAACA AGTCTGATTG TTCCGAATAC TGAAACACCA 
AGAACTGTAT CTCCAGCTAG TAAACCTCCT CTTCCACCAC AATCTGTTGA ACGGGTTGAG 
AGGACTGAAG ATGCATCACC TGCTCCACTT CCACAACTTC CGATCCGAAA GCCACCTCTG 
CCACGTCAAC AGTCCAGTAA CGATGATTCT TTGAATGAGA AAGTGGAAAC CATCCGTAGA 
GCTCATCAAC AACGTCAAGA TGCATCTAGA GCTGCATCAA GAGCAGTTTC AAGTGAAGAA 
TCAGAGGGAG AGGATTTTCC CGTATCCACC AACCGCGGTA GAATACGAAT AGTTTGTCGC 
GCGGCATCTG TAAACCGTGA TGATGGACTG ATGAGTATGA TCCCAGGAAC TGGTACGATC 
ATAAATGTTC CGCCACCTCA ACATCAACTT CCGGCATCCG CTCCGATCAC CAATGGGACA 
TCTGAACAAG TCGCGATTCC AGTCACACAT TTCACAGGTG AGGAAGACGA AAGTGACATC 
GTCGTGCCAG CGTGGTTACA GCGTCGGAGG CAACGCTTCC AACGCTCGCG CACAAATCCC 
GACATACAGG CACAGTTCAC AAGTGCTCGA GTGCAGCAGC TTCTCGCCGA ACGTCAATCT 
CGTTTAGACT CTTCTACGAC AACCGACGAA GAGAAAGAGA AGCTTGCCGT TTTGCGACAG 
CGTGGCCGCA GCGCTTCTCG TGAAGCTGGT GAATGGAGGG CTCGTGGTAG ACCACGTGCA 
GTATTCGGAC GAAAAGGTGC GAAGGATGGC GAGTTAAACT GGCCACGTGG AATTTGTGCA 
TTGTCCGGAG GTCTTGTCGC AACTTGTGAT TCGTCTAATC ATCGTGTCTG TGTTTTCGAT 
AAAGATGGAA AATTTGTTCG TCAGTTCGGA GGCTACGGTG CCGGTGCCGG ACAACTTGAT 
TCAGCTGCCG GGCTAGCAAG CTCAAAGCTC CGAGTTATTG TATCCGACAG GTACAATCAT 
CGAATTTCCG TTTTTGGATT GGAAGGAGAT CATTTGTTCT CATTCGGTGG ACATGGACAG 
GGAAATGCTA AGTTCAATAA TCCATGGGGA GTTGCTGTCG ATGATTTGGG AAGTATCTAT 
GTGGCTGATA AGGATAATCA CCGCGTCCAA GTCTTCGACA AAAATGGTCA ATTCATTGCA 
AAATTCGGAT CTTTCGGACA TCTACCTGGT CAACTAAACA GTCCATTGTT CATAGCAGTT 
AGCAGAGTTA CTCATCACGT GTATGTATCA GATTCTTCGA ATCATCGAAT CAGCGTATTC 
GATCCCCACG GTGTTCATTT GTTTTCATTC GGCGAGGAAG GTTTTCATGG TGGACAATTC 
AAATTCCCAC GTGGAATTGC TATTGATTCA CAGGAAAACC TAATAATCGC CGACTCCGGT 
AACAACAGGA TCCAAGTTTT CGACGCCCAA GGTCAATTTG TCTCCTCTTT CGGAACTTGG 
GGTGGAGGAG CTGGACAACT GAAAGGTGTC GAAGATGTTT GTGTCACTGC TGACGGTTCA 
ATTGTTGTCA CCGATCGCGA GAATCATCGC ATTCAGATCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_499028.1
CDS	106..3030
Translation	MSSSPQNEAEAREKMRELMSRPPSSRPADFVNPLEKIEQLLTCPICLDRYKQPKLLPCQHTFCYPCLESCADTLHRNLKCPECRAEHNIPYDGVKAFQPNYTLTGFLEIHLQATPESAAEIEEYIHRYNLERCKICDEKADCEPCAHCDRKACKECRQTHMDMLKRDMSRLLNQVKRLANRITEASDNLSKGVDMMTMNCETTKAEIKEYFHRYQRDLKKKEDNFLMEVDTFQATETRNMSNLRDVLEIESSNMSEAVSRLDAAIKGECSIEDSELVRMKNTFTEGLEYLRNFQPDADELFNRKLRFSAGDDAAKLPAAITTSGELCVLVPQFSGRYLPLEQSYLPRPFRLPLESDSYRVKSDERASMRDREADRTSSRHSHRNPEPDESSIRYRRRQQLEDEAWNRLRNSSAAPSLLTTSVTADSSSRTSPWAADRVTRSVEPTKSRPTSLIVPNTETPRTVSPASKPPLPPQSVERVERTEDASPAPLPQLPIRKPPLPRQQSSNDDSLNEKVETIRRAHQQRQDASRAASRAVSSEESEGEDFPVSTNRGRIRIVCRAASVNRDDGLMSMIPGTGTIINVPPPQHQLPASAPITNGTSEQVAIPVTHFTGEEDESDIVVPAWLQRRRQRFQRSRTNPDIQAQFTSARVQQLLAERQSRLDSSTTTDEEKEKLAVLRQRGRSASREAGEWRARGRPRAVFGRKGAKDGELNWPRGICALSGGLVATCDSSNHRVCVFDKDGKFVRQFGGYGAGAGQLDSAAGLASSKLRVIVSDRYNHRISVFGLEGDHLFSFGGHGQGNAKFNNPWGVAVDDLGSIYVADKDNHRVQVFDKNGQFIAKFGSFGHLPGQLNSPLFIAVSRVTHHVYVSDSSNHRISVFDPHGVHLFSFGEEGFHGGQFKFPRGIAIDSQENLIIADSGNNRIQVFDAQGQFVSSFGTWGGGAGQLKGVEDVCVTADGSIVVTDRENHRIQIF

Target ORF information:

RefSeq Version	NM_066627.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans RING finger protein nhl-1 (nhl-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066627.6

ORF Insert Sequence:

ATGAGCAGTT CTCCACAAAA TGAAGCAGAA GCTCGCGAAA AGATGCGCGA GCTGATGTCA 
CGTCCACCAT CGAGTCGACC AGCTGATTTT GTGAATCCTC TCGAAAAGAT CGAGCAGTTG 
CTGACGTGTC CGATTTGTCT CGATCGCTAC AAACAACCCA AGCTTCTACC TTGTCAACAC 
ACATTTTGCT ATCCATGCTT GGAAAGTTGT GCAGACACGT TACATCGCAA TTTGAAATGT 
CCAGAATGTC GAGCAGAACA CAATATTCCA TATGACGGAG TAAAGGCGTT TCAGCCAAAC 
TACACATTAA CCGGGTTTCT CGAGATTCAT CTGCAGGCCA CACCAGAATC GGCTGCAGAA 
ATTGAAGAGT ACATTCACAG GTACAATTTG GAACGGTGCA AGATTTGTGA TGAGAAAGCA 
GACTGTGAAC CATGTGCACA TTGTGATAGA AAAGCATGTA AAGAATGTCG ACAGACTCAT 
ATGGATATGC TTAAAAGAGA CATGTCGCGA CTTTTGAATC AGGTTAAACG GCTTGCCAAT 
CGAATTACGG AAGCATCGGA TAATTTATCA AAAGGAGTTG ATATGATGAC AATGAACTGT 
GAGACAACAA AAGCAGAAAT TAAAGAATAT TTCCACAGAT ACCAGAGAGA TCTGAAAAAG 
AAGGAAGACA ACTTTCTCAT GGAAGTTGAT ACATTTCAAG CTACTGAGAC TCGTAATATG 
AGCAATCTTC GTGATGTTCT GGAAATTGAA AGTTCTAATA TGAGTGAAGC AGTTTCTAGA 
CTGGATGCTG CAATCAAGGG AGAATGTTCA ATTGAAGATT CTGAACTTGT TCGAATGAAA 
AACACGTTTA CGGAAGGGCT TGAATATCTT AGGAACTTTC AGCCCGACGC CGACGAACTT 
TTTAATCGAA AACTCCGATT CTCAGCTGGA GATGATGCTG CTAAGCTTCC AGCAGCGATC 
ACTACTTCTG GTGAACTTTG TGTTCTAGTT CCTCAATTTT CGGGAAGATA CCTCCCTCTT 
GAACAATCAT ACCTTCCAAG ACCATTCCGT TTACCATTGG AAAGTGACAG TTATCGGGTT 
AAATCTGACG AAAGAGCAAG TATGAGAGAT CGAGAAGCTG ATAGGACATC TTCAAGGCAC 
AGTCATCGAA ATCCTGAGCC TGATGAGTCC TCGATTCGTT ACCGTCGTCG TCAGCAGCTG 
GAAGACGAAG CTTGGAATCG TCTCCGTAAT TCATCTGCTG CTCCTTCTCT TCTTACTACA 
TCAGTCACTG CGGACTCTTC ATCACGAACA TCGCCGTGGG CTGCTGATCG TGTCACCCGA 
TCAGTTGAAC CAACCAAGTC ACGGCCAACA AGTCTGATTG TTCCGAATAC TGAAACACCA 
AGAACTGTAT CTCCAGCTAG TAAACCTCCT CTTCCACCAC AATCTGTTGA ACGGGTTGAG 
AGGACTGAAG ATGCATCACC TGCTCCACTT CCACAACTTC CGATCCGAAA GCCACCTCTG 
CCACGTCAAC AGTCCAGTAA CGATGATTCT TTGAATGAGA AAGTGGAAAC CATCCGTAGA 
GCTCATCAAC AACGTCAAGA TGCATCTAGA GCTGCATCAA GAGCAGTTTC AAGTGAAGAA 
TCAGAGGGAG AGGATTTTCC CGTATCCACC AACCGCGGTA GAATACGAAT AGTTTGTCGC 
GCGGCATCTG TAAACCGTGA TGATGGACTG ATGAGTATGA TCCCAGGAAC TGGTACGATC 
ATAAATGTTC CGCCACCTCA ACATCAACTT CCGGCATCCG CTCCGATCAC CAATGGGACA 
TCTGAACAAG TCGCGATTCC AGTCACACAT TTCACAGGTG AGGAAGACGA AAGTGACATC 
GTCGTGCCAG CGTGGTTACA GCGTCGGAGG CAACGCTTCC AACGCTCGCG CACAAATCCC 
GACATACAGG CACAGTTCAC AAGTGCTCGA GTGCAGCAGC TTCTCGCCGA ACGTCAATCT 
CGTTTAGACT CTTCTACGAC AACCGACGAA GAGAAAGAGA AGCTTGCCGT TTTGCGACAG 
CGTGGCCGCA GCGCTTCTCG TGAAGCTGGT GAATGGAGGG CTCGTGGTAG ACCACGTGCA 
GTATTCGGAC GAAAAGGTGC GAAGGATGGC GAGTTAAACT GGCCACGTGG AATTTGTGCA 
TTGTCCGGAG GTCTTGTCGC AACTTGTGAT TCGTCTAATC ATCGTGTCTG TGTTTTCGAT 
AAAGATGGAA AATTTGTTCG TCAGTTCGGA GGCTACGGTG CCGGTGCCGG ACAACTTGAT 
TCAGCTGCCG GGCTAGCAAG CTCAAAGCTC CGAGTTATTG TATCCGACAG GTACAATCAT 
CGAATTTCCG TTTTTGGATT GGAAGGAGAT CATTTGTTCT CATTCGGTGG ACATGGACAG 
GGAAATGCTA AGTTCAATAA TCCATGGGGA GTTGCTGTCG ATGATTTGGG AAGTATCTAT 
GTGGCTGATA AGGATAATCA CCGCGTCCAA GTCTTCGACA AAAATGGTCA ATTCATTGCA 
AAATTCGGAT CTTTCGGACA TCTACCTGGT CAACTAAACA GTCCATTGTT CATAGCAGTT 
AGCAGAGTTA CTCATCACGT GTATGTATCA GATTCTTCGA ATCATCGAAT CAGCGTATTC 
GATCCCCACG GTGTTCATTT GTTTTCATTC GGCGAGGAAG GTTTTCATGG TGGACAATTC 
AAATTCCCAC GTGGAATTGC TATTGATTCA CAGGAAAACC TAATAATCGC CGACTCCGGT 
AACAACAGGA TCCAAGTTTT CGACGCCCAA GGTCAATTTG TCTCCTCTTT CGGAACTTGG 
GGTGGAGGAG CTGGACAACT GAAAGGTGTC GAAGATGTTT GTGTCACTGC TGACGGTTCA 
ATTGTTGTCA CCGATCGCGA GAATCATCGC ATTCAGATCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

nhl-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報