nrde-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	nrde-2
Entrez Gene ID	174589
Full Name	Nuclear RNAi defective-2 protein
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_063808.4	NP_496209.1	Nuclear RNAi defective-2 protein
NM_063808.5	NP_496209.1	Nuclear exosome regulator NRDE2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate that the heritable silencing pathway depends on at least four genes, designated as nuclear RNAi defective 1-4 (nrde-1/2/3/4).
Title: Small RNAs, RNAi and the inheritance of gene silencing in Caenorhabditis elegans.

results define NRDE-2 as a component of the nuclear RNAi machinery and demonstrate that metazoan siRNAs can silence nuclear-localized RNAs co-transcriptionally
Title: Small regulatory RNAs inhibit RNA polymerase II during the elongation phase of transcription.

The following nrde-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nrde-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa339682	NM_063808.5 Latest version!	Caenorhabditis elegans Nuclear exosome regulator NRDE2 (nrde-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa339682	NM_063808.4	Caenorhabditis elegans Nuclear RNAi defective-2 protein (nrde-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nrde-2 ( NM_063808.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496209.1 Caenorhabditis elegans Nuclear RNAi defective-2 protein (nrde-2), partial mRNA.

Price & Availability↑

CloneID	OCa339682
Clone ID Related Accession (Same CDS sequence)	NM_063808.4 , NM_063808.5
Accession Version	NM_063808.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Nuclear RNAi defective-2 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_063808.3. COMPLETENESS: incomplete on both ends.

ATGTTTCGAG CGTATGGAAA TAATGGATTG AAAAATCCAG AAAGAATTTC TGGTGAGAAT 
CCAGATCTTT ATACGCAAAC AAGGGCTGCT GTTCAACAAC GAGCGACTAC CACTCTAAAA 
AGAAACGAAA AACAGAAACT CGCCGTGCAG AATGACTCTG TTTTTCAGCA AGTTGGAATT 
GGAGAATCTG ATAGTGATGA CGATAATGGT GGTGTTCGGA TTAGAATGAG CCCCCACAGG 
TACATTGATC CAGATGATGT TTTCACTCTC CCAGAGGTGA AAAAGCAAAA TGCCTTGAGA 
GACGCGAAAA TTGCGGCCCG AGCAGCTCAG GCAACTGCTT ACAATACATT TCCGTCCGTT 
AAAAGCTTAA ATGGTTGCCA AGATCCACCA GAAACAAGTC AACAATCCAC AAGTCGTAAA 
CGTTCAGCAA GCAACAGCAG GAGTCCATCG AGATCTCACA GTCGCCGATA CGATCGGGAT 
AATGGCAGGC AACGCAGCAG GAGTCGTGAA AAGAAGAGAA GAAAGAAGGA ACGCAGACGA 
AAAAGATCCA GTAGCCGAAG CTCTAGTTCA TCTCGTTCTC GGGATAGGTC ATCTCGTGCT 
CGGGATACGT CATCTCACAC TTTAATGAAA ATGAACAAAC CTGCAAAATA TGCATTTCTC 
ACCGATGAAG AATATCGCAC GTGCGATGCG TACATTTCAT CTGCGTTTAT TACTCAAACT 
AAAAGTGATT GTGAAAACTA TACGCAAGGA GTTCCGAAGA AAGAAATTGC CAAGTGTCGA 
TTGAGTGTCA AGTTTATTGT CGGTTTGGAG CATAACAATA TTCTGTTCAA CAATATTTAT 
GGAGCGGAAT ATGCCAGAGA CAAGGAAAAC CGCCCATTCT GGGAGCAGCT GGATAGATAC 
TTGAAGGATG TTCCGAAAGA GACATTCTTT CGATATGTGC CTCCAGTCGG CGGTTACTGG 
AAGATTCGTG ACAGAGTTGA TTTATTAAAT CTTGATCACA TTGACGATGA TGTTCTAGCA 
AACGATTCGG ATAATCGAAA AGATGCGTTC ACATTTGAGC TCGAACAGGC GAAGAAAACA 
TTTTCCGAAA ATGTTCATAA CATTGATGCT CTAATCAAAG TAATTTCAAT GGAAGAAGAA 
ATGTGTCGTC GGAATGTTGG AAGTTTCTCC AGTTCAAATC CAGCAGCTCT TGCCGAACGT 
CACCAGGAAA TGGTGAAAAA AGCCATTAAA GCCGACGGTA GAAATGCAAA ACTTCGATTG 
ATGAAAATTG AATTACTCAT CAAAATGGAT CCCAACTCTC CTACTATTAT TGATGACTTC 
AAAAATCTTA CCATCACATT TCCACATGAA CCAATGGTAT GGATCAAGTA TTTGGACTAT 
ATACAATATG ATAGCAATGT GTACAATTAC AAAAAATTGA AGAATGCATT CGAAGATTGT 
ATCCGACAAG TGACTGGATT GACAAATGGA ACACTTCTTT CACATTTGAA TGCTGTTAAC 
GATAGACCAT TGCTTAGAAT GTTCCATTTA TGGATTTATA TTCGTTATTT GAAGTGGATG 
ATTTCCTGTG CACACACGCC AGTGGTACTG GCAAACATAC AGGCCACTTT TGAATACAAT 
TTTGGACTCG CTGATGTTGA AAAGCGGACG AGCACTAATT CAAAAGAACG GGAAGTCAGA 
TTGGAGGAAT TCTGGGAATC TGGTCTTCCG CGAATCGGCG ACGAAGGTGC TGTTGGTGCA 
GAGAAGATGC TTAAACAATC GGAAGAATTA TCTGATGAAG ATATTCAGAA ATTGGAAAAT 
GATGATTTTG ATATTCTTAT TTCACGCACC GAGGAAACAA TAGCGACGTG TCTTCAAGCT 
CAACGGGACG TGCAGATTTC GTGGATCGAA GTTGAACGAG AAATGATGAA CATCGATGCT 
CGCGTGAAAC GGACGAAACT GAAAGATTGT GAACTTTATG AAGATCATGT GGACGACTTA 
GAGACATGTG AACTCTGGGA CATCATTCCT TTTGATAGAA TTCGATATTA TGAAGCACCA 
GGCGATTGTG CTAATTTCGA TTTTGTACAA CCTTTTTTGG AGTTGCTCGG CGTCAAATTT 
TTAAATTCGA CGAACTGCTT CACAACAACC GAACAAATCA TTTCTGATTG GATTTCTAAT 
GATTCTACTG TTAACTTCTA CAAAACTCCG ACTTATACCG AGAAAAAGTG CTTTGAAGTT 
GGAAATAATA TTCTGAAGTT TATGCTTTAC AATCGACTCA AACTGACCGA AAACAATCCT 
GAATACTTGG ATAAGACTAT GGTGAAGTAC TTGCTGGCAA TGCTCGTCAC CGAAGCTTCG 
GAACAGGAGA AGAAATTGAA TTTCCACAGT TTTAAACTGA ATCTTAAAAA CTTGGTTGGA 
ACATTCATCA CTAAACATCC AGACATTTTC AAGCGTGCCA TGCTGAGTAA GATCACAGGA 
ATTGTCTACA TGGAAAAGTT TGTGTCTTGG TGGGAGCGTG CACTGAAAGA ACAGGAAAAA 
GTAGTGGAAG CTGACGAGAG AAGGAAGAAT TATAAAGAGA TCAAGATGGA GGAAGGCGTC 
GTAGATGATG TTAAATTCGA TGTCATATTG TTAAAGAAAG ACAAAGAACG AGTGCAAACA 
ATTCGTGATA AGATTAGGGA TATGATTGAT ATAGCTATCC CCAAATCAAC AGAAAAGCTC 
ATTCAGAGCG CTGATTCTTC ATTGCCAACG CTTCAATTAC ATCTTTATGC CAATGTTCTA 
CGTGGAAGAC TTTCTATTCT AAATCAAAAT GCTTTAGAAG AGACTCGAGA TGTTTTCTGT 
AAAGAGATTC TTGGAATTCA CACTTCGGAA TTTGAAAGTG ATGAAGCTCT CTTATTGGCA 
TTGGATCAAG GATTGAACGA ATTGCTGGAG CATTGCAAGG AGAAGGACAA TCTTGAATCG 
GTCGACAGTA TACCAGAATT ACCAAGAGCT GAGGCACTGT GCGAAGCTTT GAAAGTTGTG 
GCGGTATTTG TTTTCTTGGA CAAGATGGCA TTCAGCAGGC GTGCAGTCGA CTGCCTCATT 
GCCAACGCGA TCACCAAATT CGAGCAATTT GAAGCCAAGA AAAATGATTT CAATCGTGGA 
ACTTATGAAA AATACTGTGA TCAGATCGAT TTGAAATTCA TCACGGACAC TCTCATAACC 
TTCTTTTCGC ACAAAAAACA CCGTTTCATC TACAATGAAA ACTTCAAAAA GTTGATCTTC 
CAAGCATCGC AAGCGTTCCC ATGTGATTCG AAATATGCGA AGATGCTCGG CGAGCTGCAT 
TCCAGCGGTC GTCTGCAAGT GATGAAGCTT CAAGGATTCA CCGACAGCAG AAATAGTATT 
TTGAATGCGA AACGAGATCA GCAATTTGAT CCAGAACTTG AAACTCGTCT TCTCATGAAC 
TCGTTGACTA TTATGTTCTC TTGGATGAAT GCAGCCAACC GGATTGGAGA CGCCGGAAAT 
CAAATCCTCT ATAAAAATTG GAAACGAGAA GCAGCTAATA CACGTGATCC GGCTATCTGG 
AGACAAGTTA TAAGAGTTGC CAGTAAATTG TCACAGAAGA TTCTAAAGGA CGATGCTTAC 
ACTCGTGCAA GAGGTCAATG TACATGGGCT CTCAATTTGC ACTTTGATTA TATTGAAGCA 
AAAACTGTCC GCAAGAATGG AGATCTAATG GAGATGATCT ACCTCATTCT CGAACAAAGC 
ATGGGACAAG AGCATAGTTT ATTTGTGACC GACGAGGAAT ACATGAAAAC ACAGCAGGAA 
ATTGGACTAC AGTATTCCGA ATCCGGACGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496209.1
CDS	1..3813
Translation	MFRAYGNNGLKNPERISGENPDLYTQTRAAVQQRATTTLKRNEKQKLAVQNDSVFQQVGIGESDSDDDNGGVRIRMSPHRYIDPDDVFTLPEVKKQNALRDAKIAARAAQATAYNTFPSVKSLNGCQDPPETSQQSTSRKRSASNSRSPSRSHSRRYDRDNGRQRSRSREKKRRKKERRRKRSSSRSSSSSRSRDRSSRARDTSSHTLMKMNKPAKYAFLTDEEYRTCDAYISSAFITQTKSDCENYTQGVPKKEIAKCRLSVKFIVGLEHNNILFNNIYGAEYARDKENRPFWEQLDRYLKDVPKETFFRYVPPVGGYWKIRDRVDLLNLDHIDDDVLANDSDNRKDAFTFELEQAKKTFSENVHNIDALIKVISMEEEMCRRNVGSFSSSNPAALAERHQEMVKKAIKADGRNAKLRLMKIELLIKMDPNSPTIIDDFKNLTITFPHEPMVWIKYLDYIQYDSNVYNYKKLKNAFEDCIRQVTGLTNGTLLSHLNAVNDRPLLRMFHLWIYIRYLKWMISCAHTPVVLANIQATFEYNFGLADVEKRTSTNSKEREVRLEEFWESGLPRIGDEGAVGAEKMLKQSEELSDEDIQKLENDDFDILISRTEETIATCLQAQRDVQISWIEVEREMMNIDARVKRTKLKDCELYEDHVDDLETCELWDIIPFDRIRYYEAPGDCANFDFVQPFLELLGVKFLNSTNCFTTTEQIISDWISNDSTVNFYKTPTYTEKKCFEVGNNILKFMLYNRLKLTENNPEYLDKTMVKYLLAMLVTEASEQEKKLNFHSFKLNLKNLVGTFITKHPDIFKRAMLSKITGIVYMEKFVSWWERALKEQEKVVEADERRKNYKEIKMEEGVVDDVKFDVILLKKDKERVQTIRDKIRDMIDIAIPKSTEKLIQSADSSLPTLQLHLYANVLRGRLSILNQNALEETRDVFCKEILGIHTSEFESDEALLLALDQGLNELLEHCKEKDNLESVDSIPELPRAEALCEALKVVAVFVFLDKMAFSRRAVDCLIANAITKFEQFEAKKNDFNRGTYEKYCDQIDLKFITDTLITFFSHKKHRFIYNENFKKLIFQASQAFPCDSKYAKMLGELHSSGRLQVMKLQGFTDSRNSILNAKRDQQFDPELETRLLMNSLTIMFSWMNAANRIGDAGNQILYKNWKREAANTRDPAIWRQVIRVASKLSQKILKDDAYTRARGQCTWALNLHFDYIEAKTVRKNGDLMEMIYLILEQSMGQEHSLFVTDEEYMKTQQEIGLQYSESGR

Target ORF information:

RefSeq Version	NM_063808.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Nuclear RNAi defective-2 protein (nrde-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063808.4

ORF Insert Sequence:

ATGTTTCGAG CGTATGGAAA TAATGGATTG AAAAATCCAG AAAGAATTTC TGGTGAGAAT 
CCAGATCTTT ATACGCAAAC AAGGGCTGCT GTTCAACAAC GAGCGACTAC CACTCTAAAA 
AGAAACGAAA AACAGAAACT CGCCGTGCAG AATGACTCTG TTTTTCAGCA AGTTGGAATT 
GGAGAATCTG ATAGTGATGA CGATAATGGT GGTGTTCGGA TTAGAATGAG CCCCCACAGG 
TACATTGATC CAGATGATGT TTTCACTCTC CCAGAGGTGA AAAAGCAAAA TGCCTTGAGA 
GACGCGAAAA TTGCGGCCCG AGCAGCTCAG GCAACTGCTT ACAATACATT TCCGTCCGTT 
AAAAGCTTAA ATGGTTGCCA AGATCCACCA GAAACAAGTC AACAATCCAC AAGTCGTAAA 
CGTTCAGCAA GCAACAGCAG GAGTCCATCG AGATCTCACA GTCGCCGATA CGATCGGGAT 
AATGGCAGGC AACGCAGCAG GAGTCGTGAA AAGAAGAGAA GAAAGAAGGA ACGCAGACGA 
AAAAGATCCA GTAGCCGAAG CTCTAGTTCA TCTCGTTCTC GGGATAGGTC ATCTCGTGCT 
CGGGATACGT CATCTCACAC TTTAATGAAA ATGAACAAAC CTGCAAAATA TGCATTTCTC 
ACCGATGAAG AATATCGCAC GTGCGATGCG TACATTTCAT CTGCGTTTAT TACTCAAACT 
AAAAGTGATT GTGAAAACTA TACGCAAGGA GTTCCGAAGA AAGAAATTGC CAAGTGTCGA 
TTGAGTGTCA AGTTTATTGT CGGTTTGGAG CATAACAATA TTCTGTTCAA CAATATTTAT 
GGAGCGGAAT ATGCCAGAGA CAAGGAAAAC CGCCCATTCT GGGAGCAGCT GGATAGATAC 
TTGAAGGATG TTCCGAAAGA GACATTCTTT CGATATGTGC CTCCAGTCGG CGGTTACTGG 
AAGATTCGTG ACAGAGTTGA TTTATTAAAT CTTGATCACA TTGACGATGA TGTTCTAGCA 
AACGATTCGG ATAATCGAAA AGATGCGTTC ACATTTGAGC TCGAACAGGC GAAGAAAACA 
TTTTCCGAAA ATGTTCATAA CATTGATGCT CTAATCAAAG TAATTTCAAT GGAAGAAGAA 
ATGTGTCGTC GGAATGTTGG AAGTTTCTCC AGTTCAAATC CAGCAGCTCT TGCCGAACGT 
CACCAGGAAA TGGTGAAAAA AGCCATTAAA GCCGACGGTA GAAATGCAAA ACTTCGATTG 
ATGAAAATTG AATTACTCAT CAAAATGGAT CCCAACTCTC CTACTATTAT TGATGACTTC 
AAAAATCTTA CCATCACATT TCCACATGAA CCAATGGTAT GGATCAAGTA TTTGGACTAT 
ATACAATATG ATAGCAATGT GTACAATTAC AAAAAATTGA AGAATGCATT CGAAGATTGT 
ATCCGACAAG TGACTGGATT GACAAATGGA ACACTTCTTT CACATTTGAA TGCTGTTAAC 
GATAGACCAT TGCTTAGAAT GTTCCATTTA TGGATTTATA TTCGTTATTT GAAGTGGATG 
ATTTCCTGTG CACACACGCC AGTGGTACTG GCAAACATAC AGGCCACTTT TGAATACAAT 
TTTGGACTCG CTGATGTTGA AAAGCGGACG AGCACTAATT CAAAAGAACG GGAAGTCAGA 
TTGGAGGAAT TCTGGGAATC TGGTCTTCCG CGAATCGGCG ACGAAGGTGC TGTTGGTGCA 
GAGAAGATGC TTAAACAATC GGAAGAATTA TCTGATGAAG ATATTCAGAA ATTGGAAAAT 
GATGATTTTG ATATTCTTAT TTCACGCACC GAGGAAACAA TAGCGACGTG TCTTCAAGCT 
CAACGGGACG TGCAGATTTC GTGGATCGAA GTTGAACGAG AAATGATGAA CATCGATGCT 
CGCGTGAAAC GGACGAAACT GAAAGATTGT GAACTTTATG AAGATCATGT GGACGACTTA 
GAGACATGTG AACTCTGGGA CATCATTCCT TTTGATAGAA TTCGATATTA TGAAGCACCA 
GGCGATTGTG CTAATTTCGA TTTTGTACAA CCTTTTTTGG AGTTGCTCGG CGTCAAATTT 
TTAAATTCGA CGAACTGCTT CACAACAACC GAACAAATCA TTTCTGATTG GATTTCTAAT 
GATTCTACTG TTAACTTCTA CAAAACTCCG ACTTATACCG AGAAAAAGTG CTTTGAAGTT 
GGAAATAATA TTCTGAAGTT TATGCTTTAC AATCGACTCA AACTGACCGA AAACAATCCT 
GAATACTTGG ATAAGACTAT GGTGAAGTAC TTGCTGGCAA TGCTCGTCAC CGAAGCTTCG 
GAACAGGAGA AGAAATTGAA TTTCCACAGT TTTAAACTGA ATCTTAAAAA CTTGGTTGGA 
ACATTCATCA CTAAACATCC AGACATTTTC AAGCGTGCCA TGCTGAGTAA GATCACAGGA 
ATTGTCTACA TGGAAAAGTT TGTGTCTTGG TGGGAGCGTG CACTGAAAGA ACAGGAAAAA 
GTAGTGGAAG CTGACGAGAG AAGGAAGAAT TATAAAGAGA TCAAGATGGA GGAAGGCGTC 
GTAGATGATG TTAAATTCGA TGTCATATTG TTAAAGAAAG ACAAAGAACG AGTGCAAACA 
ATTCGTGATA AGATTAGGGA TATGATTGAT ATAGCTATCC CCAAATCAAC AGAAAAGCTC 
ATTCAGAGCG CTGATTCTTC ATTGCCAACG CTTCAATTAC ATCTTTATGC CAATGTTCTA 
CGTGGAAGAC TTTCTATTCT AAATCAAAAT GCTTTAGAAG AGACTCGAGA TGTTTTCTGT 
AAAGAGATTC TTGGAATTCA CACTTCGGAA TTTGAAAGTG ATGAAGCTCT CTTATTGGCA 
TTGGATCAAG GATTGAACGA ATTGCTGGAG CATTGCAAGG AGAAGGACAA TCTTGAATCG 
GTCGACAGTA TACCAGAATT ACCAAGAGCT GAGGCACTGT GCGAAGCTTT GAAAGTTGTG 
GCGGTATTTG TTTTCTTGGA CAAGATGGCA TTCAGCAGGC GTGCAGTCGA CTGCCTCATT 
GCCAACGCGA TCACCAAATT CGAGCAATTT GAAGCCAAGA AAAATGATTT CAATCGTGGA 
ACTTATGAAA AATACTGTGA TCAGATCGAT TTGAAATTCA TCACGGACAC TCTCATAACC 
TTCTTTTCGC ACAAAAAACA CCGTTTCATC TACAATGAAA ACTTCAAAAA GTTGATCTTC 
CAAGCATCGC AAGCGTTCCC ATGTGATTCG AAATATGCGA AGATGCTCGG CGAGCTGCAT 
TCCAGCGGTC GTCTGCAAGT GATGAAGCTT CAAGGATTCA CCGACAGCAG AAATAGTATT 
TTGAATGCGA AACGAGATCA GCAATTTGAT CCAGAACTTG AAACTCGTCT TCTCATGAAC 
TCGTTGACTA TTATGTTCTC TTGGATGAAT GCAGCCAACC GGATTGGAGA CGCCGGAAAT 
CAAATCCTCT ATAAAAATTG GAAACGAGAA GCAGCTAATA CACGTGATCC GGCTATCTGG 
AGACAAGTTA TAAGAGTTGC CAGTAAATTG TCACAGAAGA TTCTAAAGGA CGATGCTTAC 
ACTCGTGCAA GAGGTCAATG TACATGGGCT CTCAATTTGC ACTTTGATTA TATTGAAGCA 
AAAACTGTCC GCAAGAATGG AGATCTAATG GAGATGATCT ACCTCATTCT CGAACAAAGC 
ATGGGACAAG AGCATAGTTT ATTTGTGACC GACGAGGAAT ACATGAAAAC ACAGCAGGAA 
ATTGGACTAC AGTATTCCGA ATCCGGACGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrde-2 ( NM_063808.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496209.1 Caenorhabditis elegans Nuclear exosome regulator NRDE2 (nrde-2), mRNA.

Price & Availability↑

CloneID	OCa339682
Clone ID Related Accession (Same CDS sequence)	NM_063808.4 , NM_063808.5
Accession Version	NM_063808.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Nuclear exosome regulator NRDE2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_063808.4.

ATGTTTCGAG CGTATGGAAA TAATGGATTG AAAAATCCAG AAAGAATTTC TGGTGAGAAT 
CCAGATCTTT ATACGCAAAC AAGGGCTGCT GTTCAACAAC GAGCGACTAC CACTCTAAAA 
AGAAACGAAA AACAGAAACT CGCCGTGCAG AATGACTCTG TTTTTCAGCA AGTTGGAATT 
GGAGAATCTG ATAGTGATGA CGATAATGGT GGTGTTCGGA TTAGAATGAG CCCCCACAGG 
TACATTGATC CAGATGATGT TTTCACTCTC CCAGAGGTGA AAAAGCAAAA TGCCTTGAGA 
GACGCGAAAA TTGCGGCCCG AGCAGCTCAG GCAACTGCTT ACAATACATT TCCGTCCGTT 
AAAAGCTTAA ATGGTTGCCA AGATCCACCA GAAACAAGTC AACAATCCAC AAGTCGTAAA 
CGTTCAGCAA GCAACAGCAG GAGTCCATCG AGATCTCACA GTCGCCGATA CGATCGGGAT 
AATGGCAGGC AACGCAGCAG GAGTCGTGAA AAGAAGAGAA GAAAGAAGGA ACGCAGACGA 
AAAAGATCCA GTAGCCGAAG CTCTAGTTCA TCTCGTTCTC GGGATAGGTC ATCTCGTGCT 
CGGGATACGT CATCTCACAC TTTAATGAAA ATGAACAAAC CTGCAAAATA TGCATTTCTC 
ACCGATGAAG AATATCGCAC GTGCGATGCG TACATTTCAT CTGCGTTTAT TACTCAAACT 
AAAAGTGATT GTGAAAACTA TACGCAAGGA GTTCCGAAGA AAGAAATTGC CAAGTGTCGA 
TTGAGTGTCA AGTTTATTGT CGGTTTGGAG CATAACAATA TTCTGTTCAA CAATATTTAT 
GGAGCGGAAT ATGCCAGAGA CAAGGAAAAC CGCCCATTCT GGGAGCAGCT GGATAGATAC 
TTGAAGGATG TTCCGAAAGA GACATTCTTT CGATATGTGC CTCCAGTCGG CGGTTACTGG 
AAGATTCGTG ACAGAGTTGA TTTATTAAAT CTTGATCACA TTGACGATGA TGTTCTAGCA 
AACGATTCGG ATAATCGAAA AGATGCGTTC ACATTTGAGC TCGAACAGGC GAAGAAAACA 
TTTTCCGAAA ATGTTCATAA CATTGATGCT CTAATCAAAG TAATTTCAAT GGAAGAAGAA 
ATGTGTCGTC GGAATGTTGG AAGTTTCTCC AGTTCAAATC CAGCAGCTCT TGCCGAACGT 
CACCAGGAAA TGGTGAAAAA AGCCATTAAA GCCGACGGTA GAAATGCAAA ACTTCGATTG 
ATGAAAATTG AATTACTCAT CAAAATGGAT CCCAACTCTC CTACTATTAT TGATGACTTC 
AAAAATCTTA CCATCACATT TCCACATGAA CCAATGGTAT GGATCAAGTA TTTGGACTAT 
ATACAATATG ATAGCAATGT GTACAATTAC AAAAAATTGA AGAATGCATT CGAAGATTGT 
ATCCGACAAG TGACTGGATT GACAAATGGA ACACTTCTTT CACATTTGAA TGCTGTTAAC 
GATAGACCAT TGCTTAGAAT GTTCCATTTA TGGATTTATA TTCGTTATTT GAAGTGGATG 
ATTTCCTGTG CACACACGCC AGTGGTACTG GCAAACATAC AGGCCACTTT TGAATACAAT 
TTTGGACTCG CTGATGTTGA AAAGCGGACG AGCACTAATT CAAAAGAACG GGAAGTCAGA 
TTGGAGGAAT TCTGGGAATC TGGTCTTCCG CGAATCGGCG ACGAAGGTGC TGTTGGTGCA 
GAGAAGATGC TTAAACAATC GGAAGAATTA TCTGATGAAG ATATTCAGAA ATTGGAAAAT 
GATGATTTTG ATATTCTTAT TTCACGCACC GAGGAAACAA TAGCGACGTG TCTTCAAGCT 
CAACGGGACG TGCAGATTTC GTGGATCGAA GTTGAACGAG AAATGATGAA CATCGATGCT 
CGCGTGAAAC GGACGAAACT GAAAGATTGT GAACTTTATG AAGATCATGT GGACGACTTA 
GAGACATGTG AACTCTGGGA CATCATTCCT TTTGATAGAA TTCGATATTA TGAAGCACCA 
GGCGATTGTG CTAATTTCGA TTTTGTACAA CCTTTTTTGG AGTTGCTCGG CGTCAAATTT 
TTAAATTCGA CGAACTGCTT CACAACAACC GAACAAATCA TTTCTGATTG GATTTCTAAT 
GATTCTACTG TTAACTTCTA CAAAACTCCG ACTTATACCG AGAAAAAGTG CTTTGAAGTT 
GGAAATAATA TTCTGAAGTT TATGCTTTAC AATCGACTCA AACTGACCGA AAACAATCCT 
GAATACTTGG ATAAGACTAT GGTGAAGTAC TTGCTGGCAA TGCTCGTCAC CGAAGCTTCG 
GAACAGGAGA AGAAATTGAA TTTCCACAGT TTTAAACTGA ATCTTAAAAA CTTGGTTGGA 
ACATTCATCA CTAAACATCC AGACATTTTC AAGCGTGCCA TGCTGAGTAA GATCACAGGA 
ATTGTCTACA TGGAAAAGTT TGTGTCTTGG TGGGAGCGTG CACTGAAAGA ACAGGAAAAA 
GTAGTGGAAG CTGACGAGAG AAGGAAGAAT TATAAAGAGA TCAAGATGGA GGAAGGCGTC 
GTAGATGATG TTAAATTCGA TGTCATATTG TTAAAGAAAG ACAAAGAACG AGTGCAAACA 
ATTCGTGATA AGATTAGGGA TATGATTGAT ATAGCTATCC CCAAATCAAC AGAAAAGCTC 
ATTCAGAGCG CTGATTCTTC ATTGCCAACG CTTCAATTAC ATCTTTATGC CAATGTTCTA 
CGTGGAAGAC TTTCTATTCT AAATCAAAAT GCTTTAGAAG AGACTCGAGA TGTTTTCTGT 
AAAGAGATTC TTGGAATTCA CACTTCGGAA TTTGAAAGTG ATGAAGCTCT CTTATTGGCA 
TTGGATCAAG GATTGAACGA ATTGCTGGAG CATTGCAAGG AGAAGGACAA TCTTGAATCG 
GTCGACAGTA TACCAGAATT ACCAAGAGCT GAGGCACTGT GCGAAGCTTT GAAAGTTGTG 
GCGGTATTTG TTTTCTTGGA CAAGATGGCA TTCAGCAGGC GTGCAGTCGA CTGCCTCATT 
GCCAACGCGA TCACCAAATT CGAGCAATTT GAAGCCAAGA AAAATGATTT CAATCGTGGA 
ACTTATGAAA AATACTGTGA TCAGATCGAT TTGAAATTCA TCACGGACAC TCTCATAACC 
TTCTTTTCGC ACAAAAAACA CCGTTTCATC TACAATGAAA ACTTCAAAAA GTTGATCTTC 
CAAGCATCGC AAGCGTTCCC ATGTGATTCG AAATATGCGA AGATGCTCGG CGAGCTGCAT 
TCCAGCGGTC GTCTGCAAGT GATGAAGCTT CAAGGATTCA CCGACAGCAG AAATAGTATT 
TTGAATGCGA AACGAGATCA GCAATTTGAT CCAGAACTTG AAACTCGTCT TCTCATGAAC 
TCGTTGACTA TTATGTTCTC TTGGATGAAT GCAGCCAACC GGATTGGAGA CGCCGGAAAT 
CAAATCCTCT ATAAAAATTG GAAACGAGAA GCAGCTAATA CACGTGATCC GGCTATCTGG 
AGACAAGTTA TAAGAGTTGC CAGTAAATTG TCACAGAAGA TTCTAAAGGA CGATGCTTAC 
ACTCGTGCAA GAGGTCAATG TACATGGGCT CTCAATTTGC ACTTTGATTA TATTGAAGCA 
AAAACTGTCC GCAAGAATGG AGATCTAATG GAGATGATCT ACCTCATTCT CGAACAAAGC 
ATGGGACAAG AGCATAGTTT ATTTGTGACC GACGAGGAAT ACATGAAAAC ACAGCAGGAA 
ATTGGACTAC AGTATTCCGA ATCCGGACGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496209.1
CDS	29..3841
Translation	MFRAYGNNGLKNPERISGENPDLYTQTRAAVQQRATTTLKRNEKQKLAVQNDSVFQQVGIGESDSDDDNGGVRIRMSPHRYIDPDDVFTLPEVKKQNALRDAKIAARAAQATAYNTFPSVKSLNGCQDPPETSQQSTSRKRSASNSRSPSRSHSRRYDRDNGRQRSRSREKKRRKKERRRKRSSSRSSSSSRSRDRSSRARDTSSHTLMKMNKPAKYAFLTDEEYRTCDAYISSAFITQTKSDCENYTQGVPKKEIAKCRLSVKFIVGLEHNNILFNNIYGAEYARDKENRPFWEQLDRYLKDVPKETFFRYVPPVGGYWKIRDRVDLLNLDHIDDDVLANDSDNRKDAFTFELEQAKKTFSENVHNIDALIKVISMEEEMCRRNVGSFSSSNPAALAERHQEMVKKAIKADGRNAKLRLMKIELLIKMDPNSPTIIDDFKNLTITFPHEPMVWIKYLDYIQYDSNVYNYKKLKNAFEDCIRQVTGLTNGTLLSHLNAVNDRPLLRMFHLWIYIRYLKWMISCAHTPVVLANIQATFEYNFGLADVEKRTSTNSKEREVRLEEFWESGLPRIGDEGAVGAEKMLKQSEELSDEDIQKLENDDFDILISRTEETIATCLQAQRDVQISWIEVEREMMNIDARVKRTKLKDCELYEDHVDDLETCELWDIIPFDRIRYYEAPGDCANFDFVQPFLELLGVKFLNSTNCFTTTEQIISDWISNDSTVNFYKTPTYTEKKCFEVGNNILKFMLYNRLKLTENNPEYLDKTMVKYLLAMLVTEASEQEKKLNFHSFKLNLKNLVGTFITKHPDIFKRAMLSKITGIVYMEKFVSWWERALKEQEKVVEADERRKNYKEIKMEEGVVDDVKFDVILLKKDKERVQTIRDKIRDMIDIAIPKSTEKLIQSADSSLPTLQLHLYANVLRGRLSILNQNALEETRDVFCKEILGIHTSEFESDEALLLALDQGLNELLEHCKEKDNLESVDSIPELPRAEALCEALKVVAVFVFLDKMAFSRRAVDCLIANAITKFEQFEAKKNDFNRGTYEKYCDQIDLKFITDTLITFFSHKKHRFIYNENFKKLIFQASQAFPCDSKYAKMLGELHSSGRLQVMKLQGFTDSRNSILNAKRDQQFDPELETRLLMNSLTIMFSWMNAANRIGDAGNQILYKNWKREAANTRDPAIWRQVIRVASKLSQKILKDDAYTRARGQCTWALNLHFDYIEAKTVRKNGDLMEMIYLILEQSMGQEHSLFVTDEEYMKTQQEIGLQYSESGR

Target ORF information:

RefSeq Version	NM_063808.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Nuclear exosome regulator NRDE2 (nrde-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063808.5

ORF Insert Sequence:

ATGTTTCGAG CGTATGGAAA TAATGGATTG AAAAATCCAG AAAGAATTTC TGGTGAGAAT 
CCAGATCTTT ATACGCAAAC AAGGGCTGCT GTTCAACAAC GAGCGACTAC CACTCTAAAA 
AGAAACGAAA AACAGAAACT CGCCGTGCAG AATGACTCTG TTTTTCAGCA AGTTGGAATT 
GGAGAATCTG ATAGTGATGA CGATAATGGT GGTGTTCGGA TTAGAATGAG CCCCCACAGG 
TACATTGATC CAGATGATGT TTTCACTCTC CCAGAGGTGA AAAAGCAAAA TGCCTTGAGA 
GACGCGAAAA TTGCGGCCCG AGCAGCTCAG GCAACTGCTT ACAATACATT TCCGTCCGTT 
AAAAGCTTAA ATGGTTGCCA AGATCCACCA GAAACAAGTC AACAATCCAC AAGTCGTAAA 
CGTTCAGCAA GCAACAGCAG GAGTCCATCG AGATCTCACA GTCGCCGATA CGATCGGGAT 
AATGGCAGGC AACGCAGCAG GAGTCGTGAA AAGAAGAGAA GAAAGAAGGA ACGCAGACGA 
AAAAGATCCA GTAGCCGAAG CTCTAGTTCA TCTCGTTCTC GGGATAGGTC ATCTCGTGCT 
CGGGATACGT CATCTCACAC TTTAATGAAA ATGAACAAAC CTGCAAAATA TGCATTTCTC 
ACCGATGAAG AATATCGCAC GTGCGATGCG TACATTTCAT CTGCGTTTAT TACTCAAACT 
AAAAGTGATT GTGAAAACTA TACGCAAGGA GTTCCGAAGA AAGAAATTGC CAAGTGTCGA 
TTGAGTGTCA AGTTTATTGT CGGTTTGGAG CATAACAATA TTCTGTTCAA CAATATTTAT 
GGAGCGGAAT ATGCCAGAGA CAAGGAAAAC CGCCCATTCT GGGAGCAGCT GGATAGATAC 
TTGAAGGATG TTCCGAAAGA GACATTCTTT CGATATGTGC CTCCAGTCGG CGGTTACTGG 
AAGATTCGTG ACAGAGTTGA TTTATTAAAT CTTGATCACA TTGACGATGA TGTTCTAGCA 
AACGATTCGG ATAATCGAAA AGATGCGTTC ACATTTGAGC TCGAACAGGC GAAGAAAACA 
TTTTCCGAAA ATGTTCATAA CATTGATGCT CTAATCAAAG TAATTTCAAT GGAAGAAGAA 
ATGTGTCGTC GGAATGTTGG AAGTTTCTCC AGTTCAAATC CAGCAGCTCT TGCCGAACGT 
CACCAGGAAA TGGTGAAAAA AGCCATTAAA GCCGACGGTA GAAATGCAAA ACTTCGATTG 
ATGAAAATTG AATTACTCAT CAAAATGGAT CCCAACTCTC CTACTATTAT TGATGACTTC 
AAAAATCTTA CCATCACATT TCCACATGAA CCAATGGTAT GGATCAAGTA TTTGGACTAT 
ATACAATATG ATAGCAATGT GTACAATTAC AAAAAATTGA AGAATGCATT CGAAGATTGT 
ATCCGACAAG TGACTGGATT GACAAATGGA ACACTTCTTT CACATTTGAA TGCTGTTAAC 
GATAGACCAT TGCTTAGAAT GTTCCATTTA TGGATTTATA TTCGTTATTT GAAGTGGATG 
ATTTCCTGTG CACACACGCC AGTGGTACTG GCAAACATAC AGGCCACTTT TGAATACAAT 
TTTGGACTCG CTGATGTTGA AAAGCGGACG AGCACTAATT CAAAAGAACG GGAAGTCAGA 
TTGGAGGAAT TCTGGGAATC TGGTCTTCCG CGAATCGGCG ACGAAGGTGC TGTTGGTGCA 
GAGAAGATGC TTAAACAATC GGAAGAATTA TCTGATGAAG ATATTCAGAA ATTGGAAAAT 
GATGATTTTG ATATTCTTAT TTCACGCACC GAGGAAACAA TAGCGACGTG TCTTCAAGCT 
CAACGGGACG TGCAGATTTC GTGGATCGAA GTTGAACGAG AAATGATGAA CATCGATGCT 
CGCGTGAAAC GGACGAAACT GAAAGATTGT GAACTTTATG AAGATCATGT GGACGACTTA 
GAGACATGTG AACTCTGGGA CATCATTCCT TTTGATAGAA TTCGATATTA TGAAGCACCA 
GGCGATTGTG CTAATTTCGA TTTTGTACAA CCTTTTTTGG AGTTGCTCGG CGTCAAATTT 
TTAAATTCGA CGAACTGCTT CACAACAACC GAACAAATCA TTTCTGATTG GATTTCTAAT 
GATTCTACTG TTAACTTCTA CAAAACTCCG ACTTATACCG AGAAAAAGTG CTTTGAAGTT 
GGAAATAATA TTCTGAAGTT TATGCTTTAC AATCGACTCA AACTGACCGA AAACAATCCT 
GAATACTTGG ATAAGACTAT GGTGAAGTAC TTGCTGGCAA TGCTCGTCAC CGAAGCTTCG 
GAACAGGAGA AGAAATTGAA TTTCCACAGT TTTAAACTGA ATCTTAAAAA CTTGGTTGGA 
ACATTCATCA CTAAACATCC AGACATTTTC AAGCGTGCCA TGCTGAGTAA GATCACAGGA 
ATTGTCTACA TGGAAAAGTT TGTGTCTTGG TGGGAGCGTG CACTGAAAGA ACAGGAAAAA 
GTAGTGGAAG CTGACGAGAG AAGGAAGAAT TATAAAGAGA TCAAGATGGA GGAAGGCGTC 
GTAGATGATG TTAAATTCGA TGTCATATTG TTAAAGAAAG ACAAAGAACG AGTGCAAACA 
ATTCGTGATA AGATTAGGGA TATGATTGAT ATAGCTATCC CCAAATCAAC AGAAAAGCTC 
ATTCAGAGCG CTGATTCTTC ATTGCCAACG CTTCAATTAC ATCTTTATGC CAATGTTCTA 
CGTGGAAGAC TTTCTATTCT AAATCAAAAT GCTTTAGAAG AGACTCGAGA TGTTTTCTGT 
AAAGAGATTC TTGGAATTCA CACTTCGGAA TTTGAAAGTG ATGAAGCTCT CTTATTGGCA 
TTGGATCAAG GATTGAACGA ATTGCTGGAG CATTGCAAGG AGAAGGACAA TCTTGAATCG 
GTCGACAGTA TACCAGAATT ACCAAGAGCT GAGGCACTGT GCGAAGCTTT GAAAGTTGTG 
GCGGTATTTG TTTTCTTGGA CAAGATGGCA TTCAGCAGGC GTGCAGTCGA CTGCCTCATT 
GCCAACGCGA TCACCAAATT CGAGCAATTT GAAGCCAAGA AAAATGATTT CAATCGTGGA 
ACTTATGAAA AATACTGTGA TCAGATCGAT TTGAAATTCA TCACGGACAC TCTCATAACC 
TTCTTTTCGC ACAAAAAACA CCGTTTCATC TACAATGAAA ACTTCAAAAA GTTGATCTTC 
CAAGCATCGC AAGCGTTCCC ATGTGATTCG AAATATGCGA AGATGCTCGG CGAGCTGCAT 
TCCAGCGGTC GTCTGCAAGT GATGAAGCTT CAAGGATTCA CCGACAGCAG AAATAGTATT 
TTGAATGCGA AACGAGATCA GCAATTTGAT CCAGAACTTG AAACTCGTCT TCTCATGAAC 
TCGTTGACTA TTATGTTCTC TTGGATGAAT GCAGCCAACC GGATTGGAGA CGCCGGAAAT 
CAAATCCTCT ATAAAAATTG GAAACGAGAA GCAGCTAATA CACGTGATCC GGCTATCTGG 
AGACAAGTTA TAAGAGTTGC CAGTAAATTG TCACAGAAGA TTCTAAAGGA CGATGCTTAC 
ACTCGTGCAA GAGGTCAATG TACATGGGCT CTCAATTTGC ACTTTGATTA TATTGAAGCA 
AAAACTGTCC GCAAGAATGG AGATCTAATG GAGATGATCT ACCTCATTCT CGAACAAAGC 
ATGGGACAAG AGCATAGTTT ATTTGTGACC GACGAGGAAT ACATGAAAAC ACAGCAGGAA 
ATTGGACTAC AGTATTCCGA ATCCGGACGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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nrde-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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