gld-3 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	gld-3
Entrez Gene ID	174174
Full Name	Defective in germ line development protein 3
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_063079.6	NP_495480.1	Defective in germ line development protein 3
NM_063079.5	NP_495480.1	Defective in germ line development protein 3
NM_063078.3	NP_495479.2	Defective in germ line development protein 3
NM_063078.4	NP_495479.2	Defective in germ line development protein 3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

picture that emerges from the structural and biochemical data are that GLD-3 activates GLD-2 both indirectly by stabilizing the enzyme and directly by contributing positively charged residues near the RNA-binding cleft
Title: Structural basis for the activation of the C. elegans noncanonical cytoplasmic poly(A)-polymerase GLD-2 by GLD-3.

Data indicate that a 10-amino-acid region in germline development defective-3 (GLD-3) is required for fem-3 binding factor (FBF) binding, while a 7-amino-acid loop in FBF between PUF repeats 7 and 8 is necessary for GLD-3 binding.
Title: A protein.protein interaction platform involved in recruitment of GLD-3 to the FBF.fem-3 mRNA complex.

report the 2.8-A-resolution crystal structure of a proteolytically stable fragment encompassing the KH2, KH3, KH4, and KH5 domains of C. elegans GLD-3
Title: Four KH domains of the C. elegans Bicaudal-C ortholog GLD-3 form a globular structural platform.

In C. elegans hermaphrodites, gamete production begins with spermatogenesis and transitions later to oogenesis; the combinatorial use of GLD-2 contributes to this transition, as GLD-2/GLD-3 promotes spermatogenesis, and GLD-2/RNP-8 specifies oogenesis.
Title: Antagonism between GLD-2 binding partners controls gamete sex.

In germ cells, GLD-3 promotes the transition from mitosis to meiosis primarily via its interaction with GLD-2, and it promotes spermatogenesis primarily via its interaction with FBF.
Title: GLD-3 and control of the mitosis/meiosis decision in the germline of Caenorhabditis elegans.

The following gld-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gld-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa338786	NM_063079.6 Latest version!	Caenorhabditis elegans Defective in germ line development protein 3 (gld-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa338787	NM_063078.4 Latest version!	Caenorhabditis elegans Defective in germ line development protein 3 (gld-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCa338786	NM_063079.5	Caenorhabditis elegans Defective in germ line development protein 3 (gld-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCa338787	NM_063078.3	Caenorhabditis elegans Defective in germ line development protein 3 (gld-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gld-3 ( NM_063079.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495480.1 Caenorhabditis elegans Defective in germ line development protein 3 (gld-3), partial mRNA.

Price & Availability↑

CloneID	OCa338786
Clone ID Related Accession (Same CDS sequence)	NM_063079.6 , NM_063079.5
Accession Version	NM_063079.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Defective in germ line development protein 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_063079.5. COMPLETENESS: incomplete on the 5' end.

ATGGGGGAGC AAAGCCATGA AAAAGATCAT GATGAAGCGC ACTCCTACAA TCCATTCGTT 
CGCTCTGCCG TTGAATACGA TGCTGACACA CGCCTCCAAA TGGCAGAAAA TGCGGCATCT 
GCAAGAAAAC TATTTGTATC GTCTGCCCTC AAAGATATTA TCGTGAATCC TGAAAACTTC 
TATCATGATT TCCAGCAATC TGCACAGATG GCGGAGGATG CTAATCAGAG ACGACAGGTG 
TCATACAACA CGAAACGAGA GGCTCATATT CATCAGCTAA AAGCGCAAGG ATTGCCTCTG 
CCGTCAAATA TACCAATGAT CGAGATTAAC CCAACTAGAG TAACCCTCAA TATGGAGTTT 
GAATCCCAAT ACTATTCACT AATGACCAGT GACAACGGGG ATCACGAGAA TGTCGCGTCA 
ATCATGGCAG AAACTAACAC CCTCATTCAA CTTCCCGATC GTTCTGTCGG AGGAACTACA 
CCTGATCCAT TTGCACAGCA AGTAACCATC ACTGGATACT TTGGTGACGT GGATAGAGCA 
CGAATGTTGA TGAGAAGAAA CTGTCATTTT ACAGTTTTTA TGGCACTGTC AAAAATGAAG 
ATGCCACTTC ACGAACTTCA GGCACATGTC AGACAGAACC CCATTCAAAA TGTGGAAATG 
TCTTTCGTCG ATGCTCCAGA AAAGAATGGA ATCGTCACAA CCTATCTTCG TATCACCGCC 
CGCGAAAAGA ATCAGCATGA ACTGATCGAA GCTGCAAAAC GACTGAATGA AATACTTTTC 
CGCGAGTCTC CGGCACCGGA GAATAATTTC ACTCTCCATT TCACATTGTC AACATACTAC 
GTGGATCAAG TTCTTGGAAG TTCATCGACG GCGCAACTCA TGCCTGTAAT CGAGAGGGAG 
ACAACGACAA TAATTAGTTA TCCATGTTAC AATAATCGAA ACGAAACTCG TGGAAATATT 
TATGAAATTA AAGTTGTTGG GAACATTGAC AATGTTCTTA AAGCCAGGAG ATACATAATG 
GACCTCCTTC CTATTTCGAT GTGTTTCAAC ATTAAAAATA CTGATATGGC AGAACCGAGC 
AGAGTTTCTG ATCGGAACAT ACACATGATT ATTGATGAGA GTGGGATTAT TCTTAAAATG 
ACTCCTTCCG TGTATGAGCC CGCGGATCTA CTGTCAGGAG AGGTTCCGTT GAACTGTGCC 
AGTCTTCGTT CGAAGGAGTT TAACATAAAG AAGCTGTACA CCGCTTATCA AAAAGTTCTC 
TCGAAAAAAT TTGATTTCAT TGCTCCACAA CCAAATGATT ATGACAACTC CATCTGGCAT 
CATTCTCTTC CGGCAAACTT TTTGAAAAAT TTCAATATGC CGTGTCGGGG TGAACTTTCT 
GATGGTTCAA ACGGTCGTCG TCATAGAAGC TCATCAATAG CTTCTTCTCG TTCCAAACAC 
AGTTACATGA GCAAAGGAAA ACAATTTTCT GAGTCGTCTG GGGGTCCATC AAGGTCTCAC 
ACAAGAGTAT CTTCGTTTTC CGAAAATTCA TCAACCGTCC CTATTATGCA ATTTCCAACG 
CCACATTTTG CACCACCAAT GTTAACTCCT CATCATCACA TGCTGAAATA CGTCTATTTG 
CAGCAACATC AGCAAGCTCA GACATTTTTG AAAGGTGCAG CTGGATTACA TCCTGGCACA 
CATATCATGT TCCCTCCACC GATCATCGTC GATGGATCCT TTGGTGCGCG TTATTCTTCA 
AATATTTATC CATTTTCTTA CATAGTTCCA CAGAACTTTA ACTTTTTGAA CGGAAATTTC 
GGTCACCTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495480.1
CDS	1..1812
Translation	MGEQSHEKDHDEAHSYNPFVRSAVEYDADTRLQMAENAASARKLFVSSALKDIIVNPENFYHDFQQSAQMAEDANQRRQVSYNTKREAHIHQLKAQGLPLPSNIPMIEINPTRVTLNMEFESQYYSLMTSDNGDHENVASIMAETNTLIQLPDRSVGGTTPDPFAQQVTITGYFGDVDRARMLMRRNCHFTVFMALSKMKMPLHELQAHVRQNPIQNVEMSFVDAPEKNGIVTTYLRITAREKNQHELIEAAKRLNEILFRESPAPENNFTLHFTLSTYYVDQVLGSSSTAQLMPVIERETTTIISYPCYNNRNETRGNIYEIKVVGNIDNVLKARRYIMDLLPISMCFNIKNTDMAEPSRVSDRNIHMIIDESGIILKMTPSVYEPADLLSGEVPLNCASLRSKEFNIKKLYTAYQKVLSKKFDFIAPQPNDYDNSIWHHSLPANFLKNFNMPCRGELSDGSNGRRHRSSSIASSRSKHSYMSKGKQFSESSGGPSRSHTRVSSFSENSSTVPIMQFPTPHFAPPMLTPHHHMLKYVYLQQHQQAQTFLKGAAGLHPGTHIMFPPPIIVDGSFGARYSSNIYPFSYIVPQNFNFLNGNFGHL

Target ORF information:

RefSeq Version	NM_063079.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Defective in germ line development protein 3 (gld-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063079.6

ORF Insert Sequence:

ATGGGGGAGC AAAGCCATGA AAAAGATCAT GATGAAGCGC ACTCCTACAA TCCATTCGTT 
CGCTCTGCCG TTGAATACGA TGCTGACACA CGCCTCCAAA TGGCAGAAAA TGCGGCATCT 
GCAAGAAAAC TATTTGTATC GTCTGCCCTC AAAGATATTA TCGTGAATCC TGAAAACTTC 
TATCATGATT TCCAGCAATC TGCACAGATG GCGGAGGATG CTAATCAGAG ACGACAGGTG 
TCATACAACA CGAAACGAGA GGCTCATATT CATCAGCTAA AAGCGCAAGG ATTGCCTCTG 
CCGTCAAATA TACCAATGAT CGAGATTAAC CCAACTAGAG TAACCCTCAA TATGGAGTTT 
GAATCCCAAT ACTATTCACT AATGACCAGT GACAACGGGG ATCACGAGAA TGTCGCGTCA 
ATCATGGCAG AAACTAACAC CCTCATTCAA CTTCCCGATC GTTCTGTCGG AGGAACTACA 
CCTGATCCAT TTGCACAGCA AGTAACCATC ACTGGATACT TTGGTGACGT GGATAGAGCA 
CGAATGTTGA TGAGAAGAAA CTGTCATTTT ACAGTTTTTA TGGCACTGTC AAAAATGAAG 
ATGCCACTTC ACGAACTTCA GGCACATGTC AGACAGAACC CCATTCAAAA TGTGGAAATG 
TCTTTCGTCG ATGCTCCAGA AAAGAATGGA ATCGTCACAA CCTATCTTCG TATCACCGCC 
CGCGAAAAGA ATCAGCATGA ACTGATCGAA GCTGCAAAAC GACTGAATGA AATACTTTTC 
CGCGAGTCTC CGGCACCGGA GAATAATTTC ACTCTCCATT TCACATTGTC AACATACTAC 
GTGGATCAAG TTCTTGGAAG TTCATCGACG GCGCAACTCA TGCCTGTAAT CGAGAGGGAG 
ACAACGACAA TAATTAGTTA TCCATGTTAC AATAATCGAA ACGAAACTCG TGGAAATATT 
TATGAAATTA AAGTTGTTGG GAACATTGAC AATGTTCTTA AAGCCAGGAG ATACATAATG 
GACCTCCTTC CTATTTCGAT GTGTTTCAAC ATTAAAAATA CTGATATGGC AGAACCGAGC 
AGAGTTTCTG ATCGGAACAT ACACATGATT ATTGATGAGA GTGGGATTAT TCTTAAAATG 
ACTCCTTCCG TGTATGAGCC CGCGGATCTA CTGTCAGGAG AGGTTCCGTT GAACTGTGCC 
AGTCTTCGTT CGAAGGAGTT TAACATAAAG AAGCTGTACA CCGCTTATCA AAAAGTTCTC 
TCGAAAAAAT TTGATTTCAT TGCTCCACAA CCAAATGATT ATGACAACTC CATCTGGCAT 
CATTCTCTTC CGGCAAACTT TTTGAAAAAT TTCAATATGC CGTGTCGGGG TGAACTTTCT 
GATGGTTCAA ACGGTCGTCG TCATAGAAGC TCATCAATAG CTTCTTCTCG TTCCAAACAC 
AGTTACATGA GCAAAGGAAA ACAATTTTCT GAGTCGTCTG GGGGTCCATC AAGGTCTCAC 
ACAAGAGTAT CTTCGTTTTC CGAAAATTCA TCAACCGTCC CTATTATGCA ATTTCCAACG 
CCACATTTTG CACCACCAAT GTTAACTCCT CATCATCACA TGCTGAAATA CGTCTATTTG 
CAGCAACATC AGCAAGCTCA GACATTTTTG AAAGGTGCAG CTGGATTACA TCCTGGCACA 
CATATCATGT TCCCTCCACC GATCATCGTC GATGGATCCT TTGGTGCGCG TTATTCTTCA 
AATATTTATC CATTTTCTTA CATAGTTCCA CAGAACTTTA ACTTTTTGAA CGGAAATTTC 
GGTCACCTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gld-3 ( NM_063079.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495480.1 Caenorhabditis elegans Defective in germ line development protein 3 (gld-3), partial mRNA.

Price & Availability↑

CloneID	OCa338786
Clone ID Related Accession (Same CDS sequence)	NM_063079.6 , NM_063079.5
Accession Version	NM_063079.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Defective in germ line development protein 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_063079.4. COMPLETENESS: incomplete on both ends.

ATGGGGGAGC AAAGCCATGA AAAAGATCAT GATGAAGCGC ACTCCTACAA TCCATTCGTT 
CGCTCTGCCG TTGAATACGA TGCTGACACA CGCCTCCAAA TGGCAGAAAA TGCGGCATCT 
GCAAGAAAAC TATTTGTATC GTCTGCCCTC AAAGATATTA TCGTGAATCC TGAAAACTTC 
TATCATGATT TCCAGCAATC TGCACAGATG GCGGAGGATG CTAATCAGAG ACGACAGGTG 
TCATACAACA CGAAACGAGA GGCTCATATT CATCAGCTAA AAGCGCAAGG ATTGCCTCTG 
CCGTCAAATA TACCAATGAT CGAGATTAAC CCAACTAGAG TAACCCTCAA TATGGAGTTT 
GAATCCCAAT ACTATTCACT AATGACCAGT GACAACGGGG ATCACGAGAA TGTCGCGTCA 
ATCATGGCAG AAACTAACAC CCTCATTCAA CTTCCCGATC GTTCTGTCGG AGGAACTACA 
CCTGATCCAT TTGCACAGCA AGTAACCATC ACTGGATACT TTGGTGACGT GGATAGAGCA 
CGAATGTTGA TGAGAAGAAA CTGTCATTTT ACAGTTTTTA TGGCACTGTC AAAAATGAAG 
ATGCCACTTC ACGAACTTCA GGCACATGTC AGACAGAACC CCATTCAAAA TGTGGAAATG 
TCTTTCGTCG ATGCTCCAGA AAAGAATGGA ATCGTCACAA CCTATCTTCG TATCACCGCC 
CGCGAAAAGA ATCAGCATGA ACTGATCGAA GCTGCAAAAC GACTGAATGA AATACTTTTC 
CGCGAGTCTC CGGCACCGGA GAATAATTTC ACTCTCCATT TCACATTGTC AACATACTAC 
GTGGATCAAG TTCTTGGAAG TTCATCGACG GCGCAACTCA TGCCTGTAAT CGAGAGGGAG 
ACAACGACAA TAATTAGTTA TCCATGTTAC AATAATCGAA ACGAAACTCG TGGAAATATT 
TATGAAATTA AAGTTGTTGG GAACATTGAC AATGTTCTTA AAGCCAGGAG ATACATAATG 
GACCTCCTTC CTATTTCGAT GTGTTTCAAC ATTAAAAATA CTGATATGGC AGAACCGAGC 
AGAGTTTCTG ATCGGAACAT ACACATGATT ATTGATGAGA GTGGGATTAT TCTTAAAATG 
ACTCCTTCCG TGTATGAGCC CGCGGATCTA CTGTCAGGAG AGGTTCCGTT GAACTGTGCC 
AGTCTTCGTT CGAAGGAGTT TAACATAAAG AAGCTGTACA CCGCTTATCA AAAAGTTCTC 
TCGAAAAAAT TTGATTTCAT TGCTCCACAA CCAAATGATT ATGACAACTC CATCTGGCAT 
CATTCTCTTC CGGCAAACTT TTTGAAAAAT TTCAATATGC CGTGTCGGGG TGAACTTTCT 
GATGGTTCAA ACGGTCGTCG TCATAGAAGC TCATCAATAG CTTCTTCTCG TTCCAAACAC 
AGTTACATGA GCAAAGGAAA ACAATTTTCT GAGTCGTCTG GGGGTCCATC AAGGTCTCAC 
ACAAGAGTAT CTTCGTTTTC CGAAAATTCA TCAACCGTCC CTATTATGCA ATTTCCAACG 
CCACATTTTG CACCACCAAT GTTAACTCCT CATCATCACA TGCTGAAATA CGTCTATTTG 
CAGCAACATC AGCAAGCTCA GACATTTTTG AAAGGTGCAG CTGGATTACA TCCTGGCACA 
CATATCATGT TCCCTCCACC GATCATCGTC GATGGATCCT TTGGTGCGCG TTATTCTTCA 
AATATTTATC CATTTTCTTA CATAGTTCCA CAGAACTTTA ACTTTTTGAA CGGAAATTTC 
GGTCACCTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495480.1
CDS	1..1812
Translation	MGEQSHEKDHDEAHSYNPFVRSAVEYDADTRLQMAENAASARKLFVSSALKDIIVNPENFYHDFQQSAQMAEDANQRRQVSYNTKREAHIHQLKAQGLPLPSNIPMIEINPTRVTLNMEFESQYYSLMTSDNGDHENVASIMAETNTLIQLPDRSVGGTTPDPFAQQVTITGYFGDVDRARMLMRRNCHFTVFMALSKMKMPLHELQAHVRQNPIQNVEMSFVDAPEKNGIVTTYLRITAREKNQHELIEAAKRLNEILFRESPAPENNFTLHFTLSTYYVDQVLGSSSTAQLMPVIERETTTIISYPCYNNRNETRGNIYEIKVVGNIDNVLKARRYIMDLLPISMCFNIKNTDMAEPSRVSDRNIHMIIDESGIILKMTPSVYEPADLLSGEVPLNCASLRSKEFNIKKLYTAYQKVLSKKFDFIAPQPNDYDNSIWHHSLPANFLKNFNMPCRGELSDGSNGRRHRSSSIASSRSKHSYMSKGKQFSESSGGPSRSHTRVSSFSENSSTVPIMQFPTPHFAPPMLTPHHHMLKYVYLQQHQQAQTFLKGAAGLHPGTHIMFPPPIIVDGSFGARYSSNIYPFSYIVPQNFNFLNGNFGHL

Target ORF information:

RefSeq Version	NM_063079.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Defective in germ line development protein 3 (gld-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063079.5

ORF Insert Sequence:

ATGGGGGAGC AAAGCCATGA AAAAGATCAT GATGAAGCGC ACTCCTACAA TCCATTCGTT 
CGCTCTGCCG TTGAATACGA TGCTGACACA CGCCTCCAAA TGGCAGAAAA TGCGGCATCT 
GCAAGAAAAC TATTTGTATC GTCTGCCCTC AAAGATATTA TCGTGAATCC TGAAAACTTC 
TATCATGATT TCCAGCAATC TGCACAGATG GCGGAGGATG CTAATCAGAG ACGACAGGTG 
TCATACAACA CGAAACGAGA GGCTCATATT CATCAGCTAA AAGCGCAAGG ATTGCCTCTG 
CCGTCAAATA TACCAATGAT CGAGATTAAC CCAACTAGAG TAACCCTCAA TATGGAGTTT 
GAATCCCAAT ACTATTCACT AATGACCAGT GACAACGGGG ATCACGAGAA TGTCGCGTCA 
ATCATGGCAG AAACTAACAC CCTCATTCAA CTTCCCGATC GTTCTGTCGG AGGAACTACA 
CCTGATCCAT TTGCACAGCA AGTAACCATC ACTGGATACT TTGGTGACGT GGATAGAGCA 
CGAATGTTGA TGAGAAGAAA CTGTCATTTT ACAGTTTTTA TGGCACTGTC AAAAATGAAG 
ATGCCACTTC ACGAACTTCA GGCACATGTC AGACAGAACC CCATTCAAAA TGTGGAAATG 
TCTTTCGTCG ATGCTCCAGA AAAGAATGGA ATCGTCACAA CCTATCTTCG TATCACCGCC 
CGCGAAAAGA ATCAGCATGA ACTGATCGAA GCTGCAAAAC GACTGAATGA AATACTTTTC 
CGCGAGTCTC CGGCACCGGA GAATAATTTC ACTCTCCATT TCACATTGTC AACATACTAC 
GTGGATCAAG TTCTTGGAAG TTCATCGACG GCGCAACTCA TGCCTGTAAT CGAGAGGGAG 
ACAACGACAA TAATTAGTTA TCCATGTTAC AATAATCGAA ACGAAACTCG TGGAAATATT 
TATGAAATTA AAGTTGTTGG GAACATTGAC AATGTTCTTA AAGCCAGGAG ATACATAATG 
GACCTCCTTC CTATTTCGAT GTGTTTCAAC ATTAAAAATA CTGATATGGC AGAACCGAGC 
AGAGTTTCTG ATCGGAACAT ACACATGATT ATTGATGAGA GTGGGATTAT TCTTAAAATG 
ACTCCTTCCG TGTATGAGCC CGCGGATCTA CTGTCAGGAG AGGTTCCGTT GAACTGTGCC 
AGTCTTCGTT CGAAGGAGTT TAACATAAAG AAGCTGTACA CCGCTTATCA AAAAGTTCTC 
TCGAAAAAAT TTGATTTCAT TGCTCCACAA CCAAATGATT ATGACAACTC CATCTGGCAT 
CATTCTCTTC CGGCAAACTT TTTGAAAAAT TTCAATATGC CGTGTCGGGG TGAACTTTCT 
GATGGTTCAA ACGGTCGTCG TCATAGAAGC TCATCAATAG CTTCTTCTCG TTCCAAACAC 
AGTTACATGA GCAAAGGAAA ACAATTTTCT GAGTCGTCTG GGGGTCCATC AAGGTCTCAC 
ACAAGAGTAT CTTCGTTTTC CGAAAATTCA TCAACCGTCC CTATTATGCA ATTTCCAACG 
CCACATTTTG CACCACCAAT GTTAACTCCT CATCATCACA TGCTGAAATA CGTCTATTTG 
CAGCAACATC AGCAAGCTCA GACATTTTTG AAAGGTGCAG CTGGATTACA TCCTGGCACA 
CATATCATGT TCCCTCCACC GATCATCGTC GATGGATCCT TTGGTGCGCG TTATTCTTCA 
AATATTTATC CATTTTCTTA CATAGTTCCA CAGAACTTTA ACTTTTTGAA CGGAAATTTC 
GGTCACCTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gld-3 ( NM_063078.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495479.2 Caenorhabditis elegans Defective in germ line development protein 3 (gld-3), partial mRNA.

Price & Availability↑

CloneID	OCa338787
Clone ID Related Accession (Same CDS sequence)	NM_063078.3 , NM_063078.4
Accession Version	NM_063078.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Defective in germ line development protein 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_063078.2. COMPLETENESS: incomplete on both ends.

ATGGGGGAGC AAAGCCATGA AAAAGATCAT GATGAAGCGC ACTCCTACAA TCCATTCGTT 
CGCTCTGCCG TTGAATACGA TGCTGACACA CGCCTCCAAA TGGCAGAAAA TGCGGCATCT 
GCAAGAAAAC TATTTGTATC GTCTGCCCTC AAAGATATTA TCGTGAATCC TGAAAACTTC 
TATCATGATT TCCAGCAATC TGCACAGATG GCGGAGGATG CTAATCAGAG ACGACAGGTG 
TCATACAACA CGAAACGAGA GGCTCATATT CATCAGCTAA AAGCGCAAGG ATTGCCTCTG 
CCGTCAAATA TACCAATGAT CGAGATTAAC CCAACTAGAG TAACCCTCAA TATGGAGTTT 
GAATCCCAAT ACTATTCACT AATGACCAGT GACAACGGGG ATCACGAGAA TGTCGCGTCA 
ATCATGGCAG AAACTAACAC CCTCATTCAA CTTCCCGATC GTTCTGTCGG AGGAACTACA 
CCTGATCCAT TTGCACAGCA AGTAACCATC ACTGGATACT TTGGTGACGT GGATAGAGCA 
CGAATGTTGA TGAGAAGAAA CTGTCATTTT ACAGTTTTTA TGGCACTGTC AAAAATGAAG 
ATGCCACTTC ACGAACTTCA GGCACATGTC AGACAGAACC CCATTCAAAA TGTGGAAATG 
TCTTTCGTCG ATGCTCCAGA AAAGAATGGA ATCGTCACAA CCTATCTTCG TATCACCGCC 
CGCGAAAAGA ATCAGCATGA ACTGATCGAA GCTGCAAAAC GACTGAATGA AATACTTTTC 
CGCGAGTCTC CGGCACCGGA GAATAATTTC ACTCTCCATT TCACATTGTC AACATACTAC 
GTGGATCAAG TTCTTGGAAG TTCATCGACG GCGCAACTCA TGCCTGTAAT CGAGAGGGAG 
ACAACGACAA TAATTAGTTA TCCATGTTAC AATAATCGAA ACGAAACTCG TGGAAATATT 
TATGAAATTA AAGTTGTTGG GAACATTGAC AATGTTCTTA AAGCCAGGAG ATACATAATG 
GACCTCCTTC CTATTTCGAT GTGTTTCAAC ATTAAAAATA CTGATATGGC AGAACCGAGC 
AGAGTTTCTG ATCGGAACAT ACACATGATT ATTGATGAGA GTGGGATTAT TCTTAAAATG 
ACTCCTTCCG TGTATGAGCC CGCGGATCTA CTGTCAGGAG AGGTTCCGTT GAACTGTGCC 
AGTCTTCGTT CGAAGGAGTT TAACATAAAG AAGCTGTACA CCGCTTATCA AAAAGTTCTC 
TCGAAAAAAT TTGATTTCAT TGCTCCACAA CCAAATGATT ATGACAACTC CATCTGGCAT 
CATTCTCTTC CGGCAAACTT TTTGAAAAAT TTCAATATGC CGTGTCGGGG TGAACTTTCT 
GATGGTTCAA ACGGTCGTCG TCATAGAAGC TCATCAATAG CTTCTTCTCG TTCCAAACAC 
AGTTACATGA GCAAAGGAAA ACAATTTTCT GAGTCGTCTG GGGGTCCATC AAGGTCTCAC 
ACAAGAGTAT CTTCGTTTTC CGAAAATTCA TCAACCGTCC CTATTATGCA ATTTCCAACG 
CCACATTTTG CACCACCAAT GTTAACTCCT CATCATCACA TGCTGAAATA CGTCTATTTG 
CAGCAACATC AGCAAGCTCA GACATTTTTG AAAGGTGCAG CTGGATTACA TCCTGGCACA 
CATATCATGT TCCCTCCACC GATCATCGTC GATGGATCCT TTGTGTCTGC TCTGCCATTT 
GCGGATCCTG TAGTATTTGA TGGTTTTCCT TATGTCCACG GATTGTTTCC GGTAAATGAA 
GCCGAACAAC ACAGAAATCA CCGTGAATCA TCACCATCTC TACGATCAAC ACAAGAAATC 
AGAAAACCGT CTAGAAACAT GGGAAATCGA CCATCATCGA GCACAGGTTC TTATTATCCA 
TCGACAACAC CTCGTCAACG TGTTTACGAA CAAGTTCGAG AAGATGATCT TCGTTCACAT 
ATTGGTTCAA GAAGAACATC AGTGAATGGA GACGATCAGA ATGTTGAAAG CATGCACGAC 
CAAGGATATG AAAGACAGTA CCCACGACAG CATCAAAGAC TTCAGAAGGA TGATCAACAA 
CGCTGGAAGA CAGGAAGTCG TGGAGATATT CACTCAAGTA GAACAATTAA TGTGCACAGA 
GACGTTCGCA ATTCCAATGA ATACGACTTT CATGTGGGAA ATTCTGGGCC AGCTAAGAGG 
TCCCCGTCTT TGGAACAAGT ACAATTGCAA ATGACACACC ACTTGAAGTT GAAATCGAAT 
GATGTTGATT TGGATCATGA AAAGCTTTAT ATGCATGAGT CACCGCACAA TGATTCTGAT 
ACTACAGTTT CTGCAAGTGG CTTCGGAAAT GATTTAATGG ATGGAGACTT TGTTCAGAGA 
TTCCTATCCA ATGCAAATAT CAATGAATCG GGAAGAAGAC CAAGAACAGT TAGCTGTTTT 
ACCGAGAAAG ATGGTCAATC CGCCCGATAC ATCGATTCTG ATGGTGCATA CAGCGTTGTT 
GATCATGCAT CCACACATCA ATCTCGTTCA TATGATAGCT TCAGAAAAGT TGGCGACAAC 
GGAGTCACAA AAACCATATT GGAACCACGT GCTCGAGTAG AGAAAGATTA TGGCAAAATA 
AGCCTAGAAC ACAAGACGAA ATACTCAAAT GAGTACGGTG ATGAAGAGAA GTCAGCAGAA 
AATGATACGT CTTCGTTGGG ATCTAGACAA TACAGAATTG ATCCAATGAA GTTAATAGCT 
AGTGTTCGTG AATCGAGTGA GCAACTACCA CGTATTCATG AAAGGCAGTT TAGCGATGTT 
CTGAATGAAA AAGAAAAAGA GATCGCCGAC AAAAGTATTG AATCTACAGT AACCCAAGAT 
CTTTCGTTAG ACGAAACATC TACATATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495479.2
CDS	1..2910
Translation	MGEQSHEKDHDEAHSYNPFVRSAVEYDADTRLQMAENAASARKLFVSSALKDIIVNPENFYHDFQQSAQMAEDANQRRQVSYNTKREAHIHQLKAQGLPLPSNIPMIEINPTRVTLNMEFESQYYSLMTSDNGDHENVASIMAETNTLIQLPDRSVGGTTPDPFAQQVTITGYFGDVDRARMLMRRNCHFTVFMALSKMKMPLHELQAHVRQNPIQNVEMSFVDAPEKNGIVTTYLRITAREKNQHELIEAAKRLNEILFRESPAPENNFTLHFTLSTYYVDQVLGSSSTAQLMPVIERETTTIISYPCYNNRNETRGNIYEIKVVGNIDNVLKARRYIMDLLPISMCFNIKNTDMAEPSRVSDRNIHMIIDESGIILKMTPSVYEPADLLSGEVPLNCASLRSKEFNIKKLYTAYQKVLSKKFDFIAPQPNDYDNSIWHHSLPANFLKNFNMPCRGELSDGSNGRRHRSSSIASSRSKHSYMSKGKQFSESSGGPSRSHTRVSSFSENSSTVPIMQFPTPHFAPPMLTPHHHMLKYVYLQQHQQAQTFLKGAAGLHPGTHIMFPPPIIVDGSFVSALPFADPVVFDGFPYVHGLFPVNEAEQHRNHRESSPSLRSTQEIRKPSRNMGNRPSSSTGSYYPSTTPRQRVYEQVREDDLRSHIGSRRTSVNGDDQNVESMHDQGYERQYPRQHQRLQKDDQQRWKTGSRGDIHSSRTINVHRDVRNSNEYDFHVGNSGPAKRSPSLEQVQLQMTHHLKLKSNDVDLDHEKLYMHESPHNDSDTTVSASGFGNDLMDGDFVQRFLSNANINESGRRPRTVSCFTEKDGQSARYIDSDGAYSVVDHASTHQSRSYDSFRKVGDNGVTKTILEPRARVEKDYGKISLEHKTKYSNEYGDEEKSAENDTSSLGSRQYRIDPMKLIASVRESSEQLPRIHERQFSDVLNEKEKEIADKSIESTVTQDLSLDETSTY

Target ORF information:

RefSeq Version	NM_063078.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Defective in germ line development protein 3 (gld-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063078.3

ORF Insert Sequence:

ATGGGGGAGC AAAGCCATGA AAAAGATCAT GATGAAGCGC ACTCCTACAA TCCATTCGTT 
CGCTCTGCCG TTGAATACGA TGCTGACACA CGCCTCCAAA TGGCAGAAAA TGCGGCATCT 
GCAAGAAAAC TATTTGTATC GTCTGCCCTC AAAGATATTA TCGTGAATCC TGAAAACTTC 
TATCATGATT TCCAGCAATC TGCACAGATG GCGGAGGATG CTAATCAGAG ACGACAGGTG 
TCATACAACA CGAAACGAGA GGCTCATATT CATCAGCTAA AAGCGCAAGG ATTGCCTCTG 
CCGTCAAATA TACCAATGAT CGAGATTAAC CCAACTAGAG TAACCCTCAA TATGGAGTTT 
GAATCCCAAT ACTATTCACT AATGACCAGT GACAACGGGG ATCACGAGAA TGTCGCGTCA 
ATCATGGCAG AAACTAACAC CCTCATTCAA CTTCCCGATC GTTCTGTCGG AGGAACTACA 
CCTGATCCAT TTGCACAGCA AGTAACCATC ACTGGATACT TTGGTGACGT GGATAGAGCA 
CGAATGTTGA TGAGAAGAAA CTGTCATTTT ACAGTTTTTA TGGCACTGTC AAAAATGAAG 
ATGCCACTTC ACGAACTTCA GGCACATGTC AGACAGAACC CCATTCAAAA TGTGGAAATG 
TCTTTCGTCG ATGCTCCAGA AAAGAATGGA ATCGTCACAA CCTATCTTCG TATCACCGCC 
CGCGAAAAGA ATCAGCATGA ACTGATCGAA GCTGCAAAAC GACTGAATGA AATACTTTTC 
CGCGAGTCTC CGGCACCGGA GAATAATTTC ACTCTCCATT TCACATTGTC AACATACTAC 
GTGGATCAAG TTCTTGGAAG TTCATCGACG GCGCAACTCA TGCCTGTAAT CGAGAGGGAG 
ACAACGACAA TAATTAGTTA TCCATGTTAC AATAATCGAA ACGAAACTCG TGGAAATATT 
TATGAAATTA AAGTTGTTGG GAACATTGAC AATGTTCTTA AAGCCAGGAG ATACATAATG 
GACCTCCTTC CTATTTCGAT GTGTTTCAAC ATTAAAAATA CTGATATGGC AGAACCGAGC 
AGAGTTTCTG ATCGGAACAT ACACATGATT ATTGATGAGA GTGGGATTAT TCTTAAAATG 
ACTCCTTCCG TGTATGAGCC CGCGGATCTA CTGTCAGGAG AGGTTCCGTT GAACTGTGCC 
AGTCTTCGTT CGAAGGAGTT TAACATAAAG AAGCTGTACA CCGCTTATCA AAAAGTTCTC 
TCGAAAAAAT TTGATTTCAT TGCTCCACAA CCAAATGATT ATGACAACTC CATCTGGCAT 
CATTCTCTTC CGGCAAACTT TTTGAAAAAT TTCAATATGC CGTGTCGGGG TGAACTTTCT 
GATGGTTCAA ACGGTCGTCG TCATAGAAGC TCATCAATAG CTTCTTCTCG TTCCAAACAC 
AGTTACATGA GCAAAGGAAA ACAATTTTCT GAGTCGTCTG GGGGTCCATC AAGGTCTCAC 
ACAAGAGTAT CTTCGTTTTC CGAAAATTCA TCAACCGTCC CTATTATGCA ATTTCCAACG 
CCACATTTTG CACCACCAAT GTTAACTCCT CATCATCACA TGCTGAAATA CGTCTATTTG 
CAGCAACATC AGCAAGCTCA GACATTTTTG AAAGGTGCAG CTGGATTACA TCCTGGCACA 
CATATCATGT TCCCTCCACC GATCATCGTC GATGGATCCT TTGTGTCTGC TCTGCCATTT 
GCGGATCCTG TAGTATTTGA TGGTTTTCCT TATGTCCACG GATTGTTTCC GGTAAATGAA 
GCCGAACAAC ACAGAAATCA CCGTGAATCA TCACCATCTC TACGATCAAC ACAAGAAATC 
AGAAAACCGT CTAGAAACAT GGGAAATCGA CCATCATCGA GCACAGGTTC TTATTATCCA 
TCGACAACAC CTCGTCAACG TGTTTACGAA CAAGTTCGAG AAGATGATCT TCGTTCACAT 
ATTGGTTCAA GAAGAACATC AGTGAATGGA GACGATCAGA ATGTTGAAAG CATGCACGAC 
CAAGGATATG AAAGACAGTA CCCACGACAG CATCAAAGAC TTCAGAAGGA TGATCAACAA 
CGCTGGAAGA CAGGAAGTCG TGGAGATATT CACTCAAGTA GAACAATTAA TGTGCACAGA 
GACGTTCGCA ATTCCAATGA ATACGACTTT CATGTGGGAA ATTCTGGGCC AGCTAAGAGG 
TCCCCGTCTT TGGAACAAGT ACAATTGCAA ATGACACACC ACTTGAAGTT GAAATCGAAT 
GATGTTGATT TGGATCATGA AAAGCTTTAT ATGCATGAGT CACCGCACAA TGATTCTGAT 
ACTACAGTTT CTGCAAGTGG CTTCGGAAAT GATTTAATGG ATGGAGACTT TGTTCAGAGA 
TTCCTATCCA ATGCAAATAT CAATGAATCG GGAAGAAGAC CAAGAACAGT TAGCTGTTTT 
ACCGAGAAAG ATGGTCAATC CGCCCGATAC ATCGATTCTG ATGGTGCATA CAGCGTTGTT 
GATCATGCAT CCACACATCA ATCTCGTTCA TATGATAGCT TCAGAAAAGT TGGCGACAAC 
GGAGTCACAA AAACCATATT GGAACCACGT GCTCGAGTAG AGAAAGATTA TGGCAAAATA 
AGCCTAGAAC ACAAGACGAA ATACTCAAAT GAGTACGGTG ATGAAGAGAA GTCAGCAGAA 
AATGATACGT CTTCGTTGGG ATCTAGACAA TACAGAATTG ATCCAATGAA GTTAATAGCT 
AGTGTTCGTG AATCGAGTGA GCAACTACCA CGTATTCATG AAAGGCAGTT TAGCGATGTT 
CTGAATGAAA AAGAAAAAGA GATCGCCGAC AAAAGTATTG AATCTACAGT AACCCAAGAT 
CTTTCGTTAG ACGAAACATC TACATATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gld-3 ( NM_063078.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495479.2 Caenorhabditis elegans Defective in germ line development protein 3 (gld-3), partial mRNA.

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CloneID	OCa338787
Clone ID Related Accession (Same CDS sequence)	NM_063078.3 , NM_063078.4
Accession Version	NM_063078.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Defective in germ line development protein 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_063078.3. COMPLETENESS: incomplete on the 5' end.

ATGGGGGAGC AAAGCCATGA AAAAGATCAT GATGAAGCGC ACTCCTACAA TCCATTCGTT 
CGCTCTGCCG TTGAATACGA TGCTGACACA CGCCTCCAAA TGGCAGAAAA TGCGGCATCT 
GCAAGAAAAC TATTTGTATC GTCTGCCCTC AAAGATATTA TCGTGAATCC TGAAAACTTC 
TATCATGATT TCCAGCAATC TGCACAGATG GCGGAGGATG CTAATCAGAG ACGACAGGTG 
TCATACAACA CGAAACGAGA GGCTCATATT CATCAGCTAA AAGCGCAAGG ATTGCCTCTG 
CCGTCAAATA TACCAATGAT CGAGATTAAC CCAACTAGAG TAACCCTCAA TATGGAGTTT 
GAATCCCAAT ACTATTCACT AATGACCAGT GACAACGGGG ATCACGAGAA TGTCGCGTCA 
ATCATGGCAG AAACTAACAC CCTCATTCAA CTTCCCGATC GTTCTGTCGG AGGAACTACA 
CCTGATCCAT TTGCACAGCA AGTAACCATC ACTGGATACT TTGGTGACGT GGATAGAGCA 
CGAATGTTGA TGAGAAGAAA CTGTCATTTT ACAGTTTTTA TGGCACTGTC AAAAATGAAG 
ATGCCACTTC ACGAACTTCA GGCACATGTC AGACAGAACC CCATTCAAAA TGTGGAAATG 
TCTTTCGTCG ATGCTCCAGA AAAGAATGGA ATCGTCACAA CCTATCTTCG TATCACCGCC 
CGCGAAAAGA ATCAGCATGA ACTGATCGAA GCTGCAAAAC GACTGAATGA AATACTTTTC 
CGCGAGTCTC CGGCACCGGA GAATAATTTC ACTCTCCATT TCACATTGTC AACATACTAC 
GTGGATCAAG TTCTTGGAAG TTCATCGACG GCGCAACTCA TGCCTGTAAT CGAGAGGGAG 
ACAACGACAA TAATTAGTTA TCCATGTTAC AATAATCGAA ACGAAACTCG TGGAAATATT 
TATGAAATTA AAGTTGTTGG GAACATTGAC AATGTTCTTA AAGCCAGGAG ATACATAATG 
GACCTCCTTC CTATTTCGAT GTGTTTCAAC ATTAAAAATA CTGATATGGC AGAACCGAGC 
AGAGTTTCTG ATCGGAACAT ACACATGATT ATTGATGAGA GTGGGATTAT TCTTAAAATG 
ACTCCTTCCG TGTATGAGCC CGCGGATCTA CTGTCAGGAG AGGTTCCGTT GAACTGTGCC 
AGTCTTCGTT CGAAGGAGTT TAACATAAAG AAGCTGTACA CCGCTTATCA AAAAGTTCTC 
TCGAAAAAAT TTGATTTCAT TGCTCCACAA CCAAATGATT ATGACAACTC CATCTGGCAT 
CATTCTCTTC CGGCAAACTT TTTGAAAAAT TTCAATATGC CGTGTCGGGG TGAACTTTCT 
GATGGTTCAA ACGGTCGTCG TCATAGAAGC TCATCAATAG CTTCTTCTCG TTCCAAACAC 
AGTTACATGA GCAAAGGAAA ACAATTTTCT GAGTCGTCTG GGGGTCCATC AAGGTCTCAC 
ACAAGAGTAT CTTCGTTTTC CGAAAATTCA TCAACCGTCC CTATTATGCA ATTTCCAACG 
CCACATTTTG CACCACCAAT GTTAACTCCT CATCATCACA TGCTGAAATA CGTCTATTTG 
CAGCAACATC AGCAAGCTCA GACATTTTTG AAAGGTGCAG CTGGATTACA TCCTGGCACA 
CATATCATGT TCCCTCCACC GATCATCGTC GATGGATCCT TTGTGTCTGC TCTGCCATTT 
GCGGATCCTG TAGTATTTGA TGGTTTTCCT TATGTCCACG GATTGTTTCC GGTAAATGAA 
GCCGAACAAC ACAGAAATCA CCGTGAATCA TCACCATCTC TACGATCAAC ACAAGAAATC 
AGAAAACCGT CTAGAAACAT GGGAAATCGA CCATCATCGA GCACAGGTTC TTATTATCCA 
TCGACAACAC CTCGTCAACG TGTTTACGAA CAAGTTCGAG AAGATGATCT TCGTTCACAT 
ATTGGTTCAA GAAGAACATC AGTGAATGGA GACGATCAGA ATGTTGAAAG CATGCACGAC 
CAAGGATATG AAAGACAGTA CCCACGACAG CATCAAAGAC TTCAGAAGGA TGATCAACAA 
CGCTGGAAGA CAGGAAGTCG TGGAGATATT CACTCAAGTA GAACAATTAA TGTGCACAGA 
GACGTTCGCA ATTCCAATGA ATACGACTTT CATGTGGGAA ATTCTGGGCC AGCTAAGAGG 
TCCCCGTCTT TGGAACAAGT ACAATTGCAA ATGACACACC ACTTGAAGTT GAAATCGAAT 
GATGTTGATT TGGATCATGA AAAGCTTTAT ATGCATGAGT CACCGCACAA TGATTCTGAT 
ACTACAGTTT CTGCAAGTGG CTTCGGAAAT GATTTAATGG ATGGAGACTT TGTTCAGAGA 
TTCCTATCCA ATGCAAATAT CAATGAATCG GGAAGAAGAC CAAGAACAGT TAGCTGTTTT 
ACCGAGAAAG ATGGTCAATC CGCCCGATAC ATCGATTCTG ATGGTGCATA CAGCGTTGTT 
GATCATGCAT CCACACATCA ATCTCGTTCA TATGATAGCT TCAGAAAAGT TGGCGACAAC 
GGAGTCACAA AAACCATATT GGAACCACGT GCTCGAGTAG AGAAAGATTA TGGCAAAATA 
AGCCTAGAAC ACAAGACGAA ATACTCAAAT GAGTACGGTG ATGAAGAGAA GTCAGCAGAA 
AATGATACGT CTTCGTTGGG ATCTAGACAA TACAGAATTG ATCCAATGAA GTTAATAGCT 
AGTGTTCGTG AATCGAGTGA GCAACTACCA CGTATTCATG AAAGGCAGTT TAGCGATGTT 
CTGAATGAAA AAGAAAAAGA GATCGCCGAC AAAAGTATTG AATCTACAGT AACCCAAGAT 
CTTTCGTTAG ACGAAACATC TACATATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495479.2
CDS	1..2910
Translation	MGEQSHEKDHDEAHSYNPFVRSAVEYDADTRLQMAENAASARKLFVSSALKDIIVNPENFYHDFQQSAQMAEDANQRRQVSYNTKREAHIHQLKAQGLPLPSNIPMIEINPTRVTLNMEFESQYYSLMTSDNGDHENVASIMAETNTLIQLPDRSVGGTTPDPFAQQVTITGYFGDVDRARMLMRRNCHFTVFMALSKMKMPLHELQAHVRQNPIQNVEMSFVDAPEKNGIVTTYLRITAREKNQHELIEAAKRLNEILFRESPAPENNFTLHFTLSTYYVDQVLGSSSTAQLMPVIERETTTIISYPCYNNRNETRGNIYEIKVVGNIDNVLKARRYIMDLLPISMCFNIKNTDMAEPSRVSDRNIHMIIDESGIILKMTPSVYEPADLLSGEVPLNCASLRSKEFNIKKLYTAYQKVLSKKFDFIAPQPNDYDNSIWHHSLPANFLKNFNMPCRGELSDGSNGRRHRSSSIASSRSKHSYMSKGKQFSESSGGPSRSHTRVSSFSENSSTVPIMQFPTPHFAPPMLTPHHHMLKYVYLQQHQQAQTFLKGAAGLHPGTHIMFPPPIIVDGSFVSALPFADPVVFDGFPYVHGLFPVNEAEQHRNHRESSPSLRSTQEIRKPSRNMGNRPSSSTGSYYPSTTPRQRVYEQVREDDLRSHIGSRRTSVNGDDQNVESMHDQGYERQYPRQHQRLQKDDQQRWKTGSRGDIHSSRTINVHRDVRNSNEYDFHVGNSGPAKRSPSLEQVQLQMTHHLKLKSNDVDLDHEKLYMHESPHNDSDTTVSASGFGNDLMDGDFVQRFLSNANINESGRRPRTVSCFTEKDGQSARYIDSDGAYSVVDHASTHQSRSYDSFRKVGDNGVTKTILEPRARVEKDYGKISLEHKTKYSNEYGDEEKSAENDTSSLGSRQYRIDPMKLIASVRESSEQLPRIHERQFSDVLNEKEKEIADKSIESTVTQDLSLDETSTY

Target ORF information:

RefSeq Version	NM_063078.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Defective in germ line development protein 3 (gld-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063078.4

ORF Insert Sequence:

ATGGGGGAGC AAAGCCATGA AAAAGATCAT GATGAAGCGC ACTCCTACAA TCCATTCGTT 
CGCTCTGCCG TTGAATACGA TGCTGACACA CGCCTCCAAA TGGCAGAAAA TGCGGCATCT 
GCAAGAAAAC TATTTGTATC GTCTGCCCTC AAAGATATTA TCGTGAATCC TGAAAACTTC 
TATCATGATT TCCAGCAATC TGCACAGATG GCGGAGGATG CTAATCAGAG ACGACAGGTG 
TCATACAACA CGAAACGAGA GGCTCATATT CATCAGCTAA AAGCGCAAGG ATTGCCTCTG 
CCGTCAAATA TACCAATGAT CGAGATTAAC CCAACTAGAG TAACCCTCAA TATGGAGTTT 
GAATCCCAAT ACTATTCACT AATGACCAGT GACAACGGGG ATCACGAGAA TGTCGCGTCA 
ATCATGGCAG AAACTAACAC CCTCATTCAA CTTCCCGATC GTTCTGTCGG AGGAACTACA 
CCTGATCCAT TTGCACAGCA AGTAACCATC ACTGGATACT TTGGTGACGT GGATAGAGCA 
CGAATGTTGA TGAGAAGAAA CTGTCATTTT ACAGTTTTTA TGGCACTGTC AAAAATGAAG 
ATGCCACTTC ACGAACTTCA GGCACATGTC AGACAGAACC CCATTCAAAA TGTGGAAATG 
TCTTTCGTCG ATGCTCCAGA AAAGAATGGA ATCGTCACAA CCTATCTTCG TATCACCGCC 
CGCGAAAAGA ATCAGCATGA ACTGATCGAA GCTGCAAAAC GACTGAATGA AATACTTTTC 
CGCGAGTCTC CGGCACCGGA GAATAATTTC ACTCTCCATT TCACATTGTC AACATACTAC 
GTGGATCAAG TTCTTGGAAG TTCATCGACG GCGCAACTCA TGCCTGTAAT CGAGAGGGAG 
ACAACGACAA TAATTAGTTA TCCATGTTAC AATAATCGAA ACGAAACTCG TGGAAATATT 
TATGAAATTA AAGTTGTTGG GAACATTGAC AATGTTCTTA AAGCCAGGAG ATACATAATG 
GACCTCCTTC CTATTTCGAT GTGTTTCAAC ATTAAAAATA CTGATATGGC AGAACCGAGC 
AGAGTTTCTG ATCGGAACAT ACACATGATT ATTGATGAGA GTGGGATTAT TCTTAAAATG 
ACTCCTTCCG TGTATGAGCC CGCGGATCTA CTGTCAGGAG AGGTTCCGTT GAACTGTGCC 
AGTCTTCGTT CGAAGGAGTT TAACATAAAG AAGCTGTACA CCGCTTATCA AAAAGTTCTC 
TCGAAAAAAT TTGATTTCAT TGCTCCACAA CCAAATGATT ATGACAACTC CATCTGGCAT 
CATTCTCTTC CGGCAAACTT TTTGAAAAAT TTCAATATGC CGTGTCGGGG TGAACTTTCT 
GATGGTTCAA ACGGTCGTCG TCATAGAAGC TCATCAATAG CTTCTTCTCG TTCCAAACAC 
AGTTACATGA GCAAAGGAAA ACAATTTTCT GAGTCGTCTG GGGGTCCATC AAGGTCTCAC 
ACAAGAGTAT CTTCGTTTTC CGAAAATTCA TCAACCGTCC CTATTATGCA ATTTCCAACG 
CCACATTTTG CACCACCAAT GTTAACTCCT CATCATCACA TGCTGAAATA CGTCTATTTG 
CAGCAACATC AGCAAGCTCA GACATTTTTG AAAGGTGCAG CTGGATTACA TCCTGGCACA 
CATATCATGT TCCCTCCACC GATCATCGTC GATGGATCCT TTGTGTCTGC TCTGCCATTT 
GCGGATCCTG TAGTATTTGA TGGTTTTCCT TATGTCCACG GATTGTTTCC GGTAAATGAA 
GCCGAACAAC ACAGAAATCA CCGTGAATCA TCACCATCTC TACGATCAAC ACAAGAAATC 
AGAAAACCGT CTAGAAACAT GGGAAATCGA CCATCATCGA GCACAGGTTC TTATTATCCA 
TCGACAACAC CTCGTCAACG TGTTTACGAA CAAGTTCGAG AAGATGATCT TCGTTCACAT 
ATTGGTTCAA GAAGAACATC AGTGAATGGA GACGATCAGA ATGTTGAAAG CATGCACGAC 
CAAGGATATG AAAGACAGTA CCCACGACAG CATCAAAGAC TTCAGAAGGA TGATCAACAA 
CGCTGGAAGA CAGGAAGTCG TGGAGATATT CACTCAAGTA GAACAATTAA TGTGCACAGA 
GACGTTCGCA ATTCCAATGA ATACGACTTT CATGTGGGAA ATTCTGGGCC AGCTAAGAGG 
TCCCCGTCTT TGGAACAAGT ACAATTGCAA ATGACACACC ACTTGAAGTT GAAATCGAAT 
GATGTTGATT TGGATCATGA AAAGCTTTAT ATGCATGAGT CACCGCACAA TGATTCTGAT 
ACTACAGTTT CTGCAAGTGG CTTCGGAAAT GATTTAATGG ATGGAGACTT TGTTCAGAGA 
TTCCTATCCA ATGCAAATAT CAATGAATCG GGAAGAAGAC CAAGAACAGT TAGCTGTTTT 
ACCGAGAAAG ATGGTCAATC CGCCCGATAC ATCGATTCTG ATGGTGCATA CAGCGTTGTT 
GATCATGCAT CCACACATCA ATCTCGTTCA TATGATAGCT TCAGAAAAGT TGGCGACAAC 
GGAGTCACAA AAACCATATT GGAACCACGT GCTCGAGTAG AGAAAGATTA TGGCAAAATA 
AGCCTAGAAC ACAAGACGAA ATACTCAAAT GAGTACGGTG ATGAAGAGAA GTCAGCAGAA 
AATGATACGT CTTCGTTGGG ATCTAGACAA TACAGAATTG ATCCAATGAA GTTAATAGCT 
AGTGTTCGTG AATCGAGTGA GCAACTACCA CGTATTCATG AAAGGCAGTT TAGCGATGTT 
CTGAATGAAA AAGAAAAAGA GATCGCCGAC AAAAGTATTG AATCTACAGT AACCCAAGAT 
CTTTCGTTAG ACGAAACATC TACATATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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gld-3 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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