gls-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	gls-1
Entrez Gene ID	172682
Full Name	Germline survival defective-1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001263949.1	NP_001250878.1	Germline survival defective-1
NM_001263949.2	NP_001250878.1	Germline survival defective-1
NM_059964.3	NP_492365.3	Germline survival defective-1
NM_182082.5	NP_871882.2	Germline survival defective-1
NM_059964.4	NP_492365.3	Germline survival defective-1
NM_182082.6	NP_871882.2	Germline survival defective-1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gls-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gls-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335347	NM_059964.4 Latest version!	Caenorhabditis elegans Germline survival defective-1 (gls-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa335348	NM_182082.6 Latest version!	Caenorhabditis elegans Germline survival defective-1 (gls-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa336569	NM_001263949.2 Latest version!	Caenorhabditis elegans Germline survival defective-1 (gls-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa336569	NM_001263949.1	Caenorhabditis elegans Germline survival defective-1 (gls-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa335347	NM_059964.3	Caenorhabditis elegans Germline survival defective-1 (gls-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa335348	NM_182082.5	Caenorhabditis elegans Germline survival defective-1 (gls-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gls-1 ( NM_059964.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492365.3 Caenorhabditis elegans Germline survival defective-1 (gls-1), partial mRNA.

Price & Availability↑

CloneID	OCa335347
Clone ID Related Accession (Same CDS sequence)	NM_059964.3 , NM_059964.4
Accession Version	NM_059964.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Germline survival defective-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Aug 9, 2005 this sequence version replaced NM_059964.2. COMPLETENESS: incomplete on both ends.

ATGCGATGTT TGATATCTTA TTTATTTCAT TCCTTCCTTA TTTTTTTAAA ATTTATCCGT 
TCAGACGTCA CAGCTTTAAC TCTTCAAGAA AAGCGTAAGA AGTCTCGGTT AACTGGTATT 
TTGATGAAAA GTATGGCCAA TAAAAAGAAC CATCAGCAAA AGAAAAGTAC AGACGGGTCA 
ACGATGAACG GGAATAACGC AACAGCAACT GCCGCTGCAA CAACTCAGAA TTCATCACAA 
ATTGGACAAA ATTCCAATTC CAGTCATAGC GTCACCAACG ATAACACTTC CAGCAATAAC 
GCTTCGACGT CTACATCGTC CATCACCTCA ACTACCACTA CGACCACTAC AGTACCTTCT 
TCACAGCAGA GTCAATCTCA AAATCAATAT TCACATCAAA GACTTTCATC GACTTCTTCA 
ACGTCACAGC AAACTGGAAT TTCTAAATTT CCTGCCAAAA AGGCTGGGCA CAACGAGCTT 
GAGAAGACAA GCAACACTGC TAATTCACAA TCGGGTGCTT TCAAAGGAAC CACAAATAAA 
GATCGGCCGA AGGAAAAGGA GAAAAACACT CACAGTGGTA ATAGGCAAAG TCAGGTTAAC 
CATAATCATC ATCATTCAAA CCATGTTAGC GGAAATCAGC AGAACCGAAA GAATAAGAAT 
CGTGATCATT CGAACCAAAG TTATCGAGGT GGCTACACAC ATAACCACAA TAATTATCAC 
TCGTTGGAAA ATGCTAAATC TTCTGGATTT CTGTCAAATT CTTCCCTATC CTCTGCGGGA 
CAAATATCAG CGTCTTCTGC TCCTCCAGTG AGCACAACTC CGACTGCTAT TCCCATCTTT 
CAAGAATCCA AAATAGTTCA AACAGATCCA CCAGTCGAAG AAGCAAAAAA GAAGAAGGAA 
AAACCAAAAA TCAAGCGTGA TGAAGAGCCG ATGCCATATA AGAGCACTGA TCCGAAAAAT 
ATGGATTCTG TGATGGCATT CAAAGAGCAC GATGAATGGG ATAAGGTGGA GTTTATGTGT 
GAACTTGTTT CTTTCCTGTC TCCAACCGAT CTTCGCCTGC TTGGAAATTG TATCGAAGGA 
TCTGTTCGTT GCTATAACAA TCAAATGCGG CCAGTAGAAA AGACTTCAAA CTGTTCGGAT 
CCAACAGCTG GTTTACCTCA ATTCGTTTGC TCTCCTCCAC CTCCACCGCA ACAAGTTTAT 
TATTTTCCTG GTATTGCGGA TGCTGCCCTG TATCAAACAA GAAATGCAGT TAACTCGGTT 
TTTGTACAGC AACATCCACC TGGTCTTCCA CCGTTATTAG GCGGAATGCA GAATATTATG 
TATCCACCTG ATGCTAACTT TCATCATTCA ACCTCTTGTA CGACACCTTC ATCATCAGTT 
TCCGTTGCAA ATAAGGATGT TAACAATGTA CCAGTTGTGC TATCAAGTAG TGTGAACGGA 
ATAAGCAATA ATATTCCATC AGATAGACAA CAGCTAGATT CCAAACCAAA TACTGCAAGA 
GGTTCGAGCG GCAATATCAA CCAATCTAAT ACAACATCAC CAGAACCGGA GCCCGGTTCT 
AATCACATCT CCACCACAGC AGCAAACCCT GCACATCCAA CTTCAGTGAC TGTACCACAG 
TCGGTACCAC CGGTCCCTCA AGAACCAGAA TTGTTTCTCA AATCTGTTCT CGATCTTACC 
AGTTACATAT ACACTCTCAT GGCAGTTTGT TCATCAACAA ATAGAAAATC AGCTGCTAAG 
ATCTCTGACT ATGTTCAAAA TGTTATTCTG AGAGAAAAGA GCCAAATACT CGAACGTATT 
CCGGATGAAC TTGATAAAAT TACCGTTTTA CAAGAAATCG GTAAAATTGT CGCAGCCATG 
ACTCATCACC CAGCAATTAC TCTTGATGAC AAAATGAAAT ATGCGGCTCT GCGGGACGGA 
TTACGTGCTC AAATAGAAAC ATTGTTTAGA CAATACTACA CAACTCAGAA ACAGTTGGAG 
CAAAGCAATC TTGTTGTACC ATCATCACAA GCTGTTGGCG ATGAGAATGA TACTGATTCC 
GACCATGAAT CAGAGGAAGA ATTTGAGCCA CTATCAGGTG TTATGGGTTC GCGTTTCGAG 
AGTAATCCAG TTCAGCCGTC GCCATCAGTT CCGGGAACAT TCTTTATCAT TCGATTTATT 
GGACGACAAA TTGAGAAGAA CGATAATCTT TTCAGCCTGG AGATTCATTG GAGTGATGGT 
GACAGAACAT TTGCTCAAAG ATCTCGTGAT CAATTGAAAG CTCTACAGCA TCGTCTTCTT 
GATGAGTTCG GACAACAGAG AAGTGAAAAA TATCTTCATC AAGGATGCAC TACATCCTAC 
TCTTCATTTG ACGATGATAA CAAAAAGCTT TCAGCGAGCA CATCTACAAT GGAGACGTTT 
GCTCCAAACG GAGAGCGTAT TGTTCCACGT CTTGCTCGTG ATGCGACTCC AGCTCAATAT 
GTTCAATACA TCAATGAACT TTCTGACTTG CCAGCTAGAA TGATGCTGTC AGCTGTAATA 
TGTGAAGAGT TCAACGGTAC CAGAGCGAAA ACAGAAGATG AAACTCGCGA AGCATCGGAT 
GGATTAATAT ATTCTCGATG GAAGAATCCA CGTGCCAAAT CTCCAGTTCG TTACTTCAAA 
CGAAATGCAA CAGGAAGCAT TGACCCGATT GAATTGCCTG TCAATATGCA ACCATTCCTT 
TATTCAAATA TCCCGCAAAC ACAAGTGCAG ACGCTGTTCC CAAGTTGCTC TAATTGTGGC 
GGTCCTCACG CTCCGAAACA CTGTGAAAAA CAAACATTGT TATCGAAGAA AGGTGATCAT 
AGAACTCGTT CAGACGGGGA AGGTTCTCAG CAAAATGGTG GAACTTCCTC TTCCAATTCA 
TACGCTCCAA TTCATGCAAA CCTCCCCCAT GCAGTATACA TTGAAAATCC GCAAGCAATG 
TTAGGATCAA ATCACTTCAA TCATCATCAA CAACAGCACA TAATTCAGAA TACTATATTC 
CATAATGGTG GACAATTTCG TGCAAACGGA ACATATGAAC CGCAAATTCC GATAGCATAC 
TATCATGCCC AGGGAACTGT ACAAAACTCA AATAATACTG GTGGAGTCAA CGGAAACAGT 
GGGGGCGGGA ACCAGAATTC GAATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492365.3
CDS	1..3150
Translation	MRCLISYLFHSFLIFLKFIRSDVTALTLQEKRKKSRLTGILMKSMANKKNHQQKKSTDGSTMNGNNATATAAATTQNSSQIGQNSNSSHSVTNDNTSSNNASTSTSSITSTTTTTTTVPSSQQSQSQNQYSHQRLSSTSSTSQQTGISKFPAKKAGHNELEKTSNTANSQSGAFKGTTNKDRPKEKEKNTHSGNRQSQVNHNHHHSNHVSGNQQNRKNKNRDHSNQSYRGGYTHNHNNYHSLENAKSSGFLSNSSLSSAGQISASSAPPVSTTPTAIPIFQESKIVQTDPPVEEAKKKKEKPKIKRDEEPMPYKSTDPKNMDSVMAFKEHDEWDKVEFMCELVSFLSPTDLRLLGNCIEGSVRCYNNQMRPVEKTSNCSDPTAGLPQFVCSPPPPPQQVYYFPGIADAALYQTRNAVNSVFVQQHPPGLPPLLGGMQNIMYPPDANFHHSTSCTTPSSSVSVANKDVNNVPVVLSSSVNGISNNIPSDRQQLDSKPNTARGSSGNINQSNTTSPEPEPGSNHISTTAANPAHPTSVTVPQSVPPVPQEPELFLKSVLDLTSYIYTLMAVCSSTNRKSAAKISDYVQNVILREKSQILERIPDELDKITVLQEIGKIVAAMTHHPAITLDDKMKYAALRDGLRAQIETLFRQYYTTQKQLEQSNLVVPSSQAVGDENDTDSDHESEEEFEPLSGVMGSRFESNPVQPSPSVPGTFFIIRFIGRQIEKNDNLFSLEIHWSDGDRTFAQRSRDQLKALQHRLLDEFGQQRSEKYLHQGCTTSYSSFDDDNKKLSASTSTMETFAPNGERIVPRLARDATPAQYVQYINELSDLPARMMLSAVICEEFNGTRAKTEDETREASDGLIYSRWKNPRAKSPVRYFKRNATGSIDPIELPVNMQPFLYSNIPQTQVQTLFPSCSNCGGPHAPKHCEKQTLLSKKGDHRTRSDGEGSQQNGGTSSSNSYAPIHANLPHAVYIENPQAMLGSNHFNHHQQQHIIQNTIFHNGGQFRANGTYEPQIPIAYYHAQGTVQNSNNTGGVNGNSGGGNQNSNF

Target ORF information:

RefSeq Version	NM_059964.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Germline survival defective-1 (gls-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059964.3

ORF Insert Sequence:

ATGCGATGTT TGATATCTTA TTTATTTCAT TCCTTCCTTA TTTTTTTAAA ATTTATCCGT 
TCAGACGTCA CAGCTTTAAC TCTTCAAGAA AAGCGTAAGA AGTCTCGGTT AACTGGTATT 
TTGATGAAAA GTATGGCCAA TAAAAAGAAC CATCAGCAAA AGAAAAGTAC AGACGGGTCA 
ACGATGAACG GGAATAACGC AACAGCAACT GCCGCTGCAA CAACTCAGAA TTCATCACAA 
ATTGGACAAA ATTCCAATTC CAGTCATAGC GTCACCAACG ATAACACTTC CAGCAATAAC 
GCTTCGACGT CTACATCGTC CATCACCTCA ACTACCACTA CGACCACTAC AGTACCTTCT 
TCACAGCAGA GTCAATCTCA AAATCAATAT TCACATCAAA GACTTTCATC GACTTCTTCA 
ACGTCACAGC AAACTGGAAT TTCTAAATTT CCTGCCAAAA AGGCTGGGCA CAACGAGCTT 
GAGAAGACAA GCAACACTGC TAATTCACAA TCGGGTGCTT TCAAAGGAAC CACAAATAAA 
GATCGGCCGA AGGAAAAGGA GAAAAACACT CACAGTGGTA ATAGGCAAAG TCAGGTTAAC 
CATAATCATC ATCATTCAAA CCATGTTAGC GGAAATCAGC AGAACCGAAA GAATAAGAAT 
CGTGATCATT CGAACCAAAG TTATCGAGGT GGCTACACAC ATAACCACAA TAATTATCAC 
TCGTTGGAAA ATGCTAAATC TTCTGGATTT CTGTCAAATT CTTCCCTATC CTCTGCGGGA 
CAAATATCAG CGTCTTCTGC TCCTCCAGTG AGCACAACTC CGACTGCTAT TCCCATCTTT 
CAAGAATCCA AAATAGTTCA AACAGATCCA CCAGTCGAAG AAGCAAAAAA GAAGAAGGAA 
AAACCAAAAA TCAAGCGTGA TGAAGAGCCG ATGCCATATA AGAGCACTGA TCCGAAAAAT 
ATGGATTCTG TGATGGCATT CAAAGAGCAC GATGAATGGG ATAAGGTGGA GTTTATGTGT 
GAACTTGTTT CTTTCCTGTC TCCAACCGAT CTTCGCCTGC TTGGAAATTG TATCGAAGGA 
TCTGTTCGTT GCTATAACAA TCAAATGCGG CCAGTAGAAA AGACTTCAAA CTGTTCGGAT 
CCAACAGCTG GTTTACCTCA ATTCGTTTGC TCTCCTCCAC CTCCACCGCA ACAAGTTTAT 
TATTTTCCTG GTATTGCGGA TGCTGCCCTG TATCAAACAA GAAATGCAGT TAACTCGGTT 
TTTGTACAGC AACATCCACC TGGTCTTCCA CCGTTATTAG GCGGAATGCA GAATATTATG 
TATCCACCTG ATGCTAACTT TCATCATTCA ACCTCTTGTA CGACACCTTC ATCATCAGTT 
TCCGTTGCAA ATAAGGATGT TAACAATGTA CCAGTTGTGC TATCAAGTAG TGTGAACGGA 
ATAAGCAATA ATATTCCATC AGATAGACAA CAGCTAGATT CCAAACCAAA TACTGCAAGA 
GGTTCGAGCG GCAATATCAA CCAATCTAAT ACAACATCAC CAGAACCGGA GCCCGGTTCT 
AATCACATCT CCACCACAGC AGCAAACCCT GCACATCCAA CTTCAGTGAC TGTACCACAG 
TCGGTACCAC CGGTCCCTCA AGAACCAGAA TTGTTTCTCA AATCTGTTCT CGATCTTACC 
AGTTACATAT ACACTCTCAT GGCAGTTTGT TCATCAACAA ATAGAAAATC AGCTGCTAAG 
ATCTCTGACT ATGTTCAAAA TGTTATTCTG AGAGAAAAGA GCCAAATACT CGAACGTATT 
CCGGATGAAC TTGATAAAAT TACCGTTTTA CAAGAAATCG GTAAAATTGT CGCAGCCATG 
ACTCATCACC CAGCAATTAC TCTTGATGAC AAAATGAAAT ATGCGGCTCT GCGGGACGGA 
TTACGTGCTC AAATAGAAAC ATTGTTTAGA CAATACTACA CAACTCAGAA ACAGTTGGAG 
CAAAGCAATC TTGTTGTACC ATCATCACAA GCTGTTGGCG ATGAGAATGA TACTGATTCC 
GACCATGAAT CAGAGGAAGA ATTTGAGCCA CTATCAGGTG TTATGGGTTC GCGTTTCGAG 
AGTAATCCAG TTCAGCCGTC GCCATCAGTT CCGGGAACAT TCTTTATCAT TCGATTTATT 
GGACGACAAA TTGAGAAGAA CGATAATCTT TTCAGCCTGG AGATTCATTG GAGTGATGGT 
GACAGAACAT TTGCTCAAAG ATCTCGTGAT CAATTGAAAG CTCTACAGCA TCGTCTTCTT 
GATGAGTTCG GACAACAGAG AAGTGAAAAA TATCTTCATC AAGGATGCAC TACATCCTAC 
TCTTCATTTG ACGATGATAA CAAAAAGCTT TCAGCGAGCA CATCTACAAT GGAGACGTTT 
GCTCCAAACG GAGAGCGTAT TGTTCCACGT CTTGCTCGTG ATGCGACTCC AGCTCAATAT 
GTTCAATACA TCAATGAACT TTCTGACTTG CCAGCTAGAA TGATGCTGTC AGCTGTAATA 
TGTGAAGAGT TCAACGGTAC CAGAGCGAAA ACAGAAGATG AAACTCGCGA AGCATCGGAT 
GGATTAATAT ATTCTCGATG GAAGAATCCA CGTGCCAAAT CTCCAGTTCG TTACTTCAAA 
CGAAATGCAA CAGGAAGCAT TGACCCGATT GAATTGCCTG TCAATATGCA ACCATTCCTT 
TATTCAAATA TCCCGCAAAC ACAAGTGCAG ACGCTGTTCC CAAGTTGCTC TAATTGTGGC 
GGTCCTCACG CTCCGAAACA CTGTGAAAAA CAAACATTGT TATCGAAGAA AGGTGATCAT 
AGAACTCGTT CAGACGGGGA AGGTTCTCAG CAAAATGGTG GAACTTCCTC TTCCAATTCA 
TACGCTCCAA TTCATGCAAA CCTCCCCCAT GCAGTATACA TTGAAAATCC GCAAGCAATG 
TTAGGATCAA ATCACTTCAA TCATCATCAA CAACAGCACA TAATTCAGAA TACTATATTC 
CATAATGGTG GACAATTTCG TGCAAACGGA ACATATGAAC CGCAAATTCC GATAGCATAC 
TATCATGCCC AGGGAACTGT ACAAAACTCA AATAATACTG GTGGAGTCAA CGGAAACAGT 
GGGGGCGGGA ACCAGAATTC GAATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gls-1 ( NM_059964.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492365.3 Caenorhabditis elegans Germline survival defective-1 (gls-1), partial mRNA.

Price & Availability↑

CloneID	OCa335347
Clone ID Related Accession (Same CDS sequence)	NM_059964.3 , NM_059964.4
Accession Version	NM_059964.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Germline survival defective-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059964.3. COMPLETENESS: incomplete on the 5' end.

ATGCGATGTT TGATATCTTA TTTATTTCAT TCCTTCCTTA TTTTTTTAAA ATTTATCCGT 
TCAGACGTCA CAGCTTTAAC TCTTCAAGAA AAGCGTAAGA AGTCTCGGTT AACTGGTATT 
TTGATGAAAA GTATGGCCAA TAAAAAGAAC CATCAGCAAA AGAAAAGTAC AGACGGGTCA 
ACGATGAACG GGAATAACGC AACAGCAACT GCCGCTGCAA CAACTCAGAA TTCATCACAA 
ATTGGACAAA ATTCCAATTC CAGTCATAGC GTCACCAACG ATAACACTTC CAGCAATAAC 
GCTTCGACGT CTACATCGTC CATCACCTCA ACTACCACTA CGACCACTAC AGTACCTTCT 
TCACAGCAGA GTCAATCTCA AAATCAATAT TCACATCAAA GACTTTCATC GACTTCTTCA 
ACGTCACAGC AAACTGGAAT TTCTAAATTT CCTGCCAAAA AGGCTGGGCA CAACGAGCTT 
GAGAAGACAA GCAACACTGC TAATTCACAA TCGGGTGCTT TCAAAGGAAC CACAAATAAA 
GATCGGCCGA AGGAAAAGGA GAAAAACACT CACAGTGGTA ATAGGCAAAG TCAGGTTAAC 
CATAATCATC ATCATTCAAA CCATGTTAGC GGAAATCAGC AGAACCGAAA GAATAAGAAT 
CGTGATCATT CGAACCAAAG TTATCGAGGT GGCTACACAC ATAACCACAA TAATTATCAC 
TCGTTGGAAA ATGCTAAATC TTCTGGATTT CTGTCAAATT CTTCCCTATC CTCTGCGGGA 
CAAATATCAG CGTCTTCTGC TCCTCCAGTG AGCACAACTC CGACTGCTAT TCCCATCTTT 
CAAGAATCCA AAATAGTTCA AACAGATCCA CCAGTCGAAG AAGCAAAAAA GAAGAAGGAA 
AAACCAAAAA TCAAGCGTGA TGAAGAGCCG ATGCCATATA AGAGCACTGA TCCGAAAAAT 
ATGGATTCTG TGATGGCATT CAAAGAGCAC GATGAATGGG ATAAGGTGGA GTTTATGTGT 
GAACTTGTTT CTTTCCTGTC TCCAACCGAT CTTCGCCTGC TTGGAAATTG TATCGAAGGA 
TCTGTTCGTT GCTATAACAA TCAAATGCGG CCAGTAGAAA AGACTTCAAA CTGTTCGGAT 
CCAACAGCTG GTTTACCTCA ATTCGTTTGC TCTCCTCCAC CTCCACCGCA ACAAGTTTAT 
TATTTTCCTG GTATTGCGGA TGCTGCCCTG TATCAAACAA GAAATGCAGT TAACTCGGTT 
TTTGTACAGC AACATCCACC TGGTCTTCCA CCGTTATTAG GCGGAATGCA GAATATTATG 
TATCCACCTG ATGCTAACTT TCATCATTCA ACCTCTTGTA CGACACCTTC ATCATCAGTT 
TCCGTTGCAA ATAAGGATGT TAACAATGTA CCAGTTGTGC TATCAAGTAG TGTGAACGGA 
ATAAGCAATA ATATTCCATC AGATAGACAA CAGCTAGATT CCAAACCAAA TACTGCAAGA 
GGTTCGAGCG GCAATATCAA CCAATCTAAT ACAACATCAC CAGAACCGGA GCCCGGTTCT 
AATCACATCT CCACCACAGC AGCAAACCCT GCACATCCAA CTTCAGTGAC TGTACCACAG 
TCGGTACCAC CGGTCCCTCA AGAACCAGAA TTGTTTCTCA AATCTGTTCT CGATCTTACC 
AGTTACATAT ACACTCTCAT GGCAGTTTGT TCATCAACAA ATAGAAAATC AGCTGCTAAG 
ATCTCTGACT ATGTTCAAAA TGTTATTCTG AGAGAAAAGA GCCAAATACT CGAACGTATT 
CCGGATGAAC TTGATAAAAT TACCGTTTTA CAAGAAATCG GTAAAATTGT CGCAGCCATG 
ACTCATCACC CAGCAATTAC TCTTGATGAC AAAATGAAAT ATGCGGCTCT GCGGGACGGA 
TTACGTGCTC AAATAGAAAC ATTGTTTAGA CAATACTACA CAACTCAGAA ACAGTTGGAG 
CAAAGCAATC TTGTTGTACC ATCATCACAA GCTGTTGGCG ATGAGAATGA TACTGATTCC 
GACCATGAAT CAGAGGAAGA ATTTGAGCCA CTATCAGGTG TTATGGGTTC GCGTTTCGAG 
AGTAATCCAG TTCAGCCGTC GCCATCAGTT CCGGGAACAT TCTTTATCAT TCGATTTATT 
GGACGACAAA TTGAGAAGAA CGATAATCTT TTCAGCCTGG AGATTCATTG GAGTGATGGT 
GACAGAACAT TTGCTCAAAG ATCTCGTGAT CAATTGAAAG CTCTACAGCA TCGTCTTCTT 
GATGAGTTCG GACAACAGAG AAGTGAAAAA TATCTTCATC AAGGATGCAC TACATCCTAC 
TCTTCATTTG ACGATGATAA CAAAAAGCTT TCAGCGAGCA CATCTACAAT GGAGACGTTT 
GCTCCAAACG GAGAGCGTAT TGTTCCACGT CTTGCTCGTG ATGCGACTCC AGCTCAATAT 
GTTCAATACA TCAATGAACT TTCTGACTTG CCAGCTAGAA TGATGCTGTC AGCTGTAATA 
TGTGAAGAGT TCAACGGTAC CAGAGCGAAA ACAGAAGATG AAACTCGCGA AGCATCGGAT 
GGATTAATAT ATTCTCGATG GAAGAATCCA CGTGCCAAAT CTCCAGTTCG TTACTTCAAA 
CGAAATGCAA CAGGAAGCAT TGACCCGATT GAATTGCCTG TCAATATGCA ACCATTCCTT 
TATTCAAATA TCCCGCAAAC ACAAGTGCAG ACGCTGTTCC CAAGTTGCTC TAATTGTGGC 
GGTCCTCACG CTCCGAAACA CTGTGAAAAA CAAACATTGT TATCGAAGAA AGGTGATCAT 
AGAACTCGTT CAGACGGGGA AGGTTCTCAG CAAAATGGTG GAACTTCCTC TTCCAATTCA 
TACGCTCCAA TTCATGCAAA CCTCCCCCAT GCAGTATACA TTGAAAATCC GCAAGCAATG 
TTAGGATCAA ATCACTTCAA TCATCATCAA CAACAGCACA TAATTCAGAA TACTATATTC 
CATAATGGTG GACAATTTCG TGCAAACGGA ACATATGAAC CGCAAATTCC GATAGCATAC 
TATCATGCCC AGGGAACTGT ACAAAACTCA AATAATACTG GTGGAGTCAA CGGAAACAGT 
GGGGGCGGGA ACCAGAATTC GAATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492365.3
CDS	1..3150
Translation	MRCLISYLFHSFLIFLKFIRSDVTALTLQEKRKKSRLTGILMKSMANKKNHQQKKSTDGSTMNGNNATATAAATTQNSSQIGQNSNSSHSVTNDNTSSNNASTSTSSITSTTTTTTTVPSSQQSQSQNQYSHQRLSSTSSTSQQTGISKFPAKKAGHNELEKTSNTANSQSGAFKGTTNKDRPKEKEKNTHSGNRQSQVNHNHHHSNHVSGNQQNRKNKNRDHSNQSYRGGYTHNHNNYHSLENAKSSGFLSNSSLSSAGQISASSAPPVSTTPTAIPIFQESKIVQTDPPVEEAKKKKEKPKIKRDEEPMPYKSTDPKNMDSVMAFKEHDEWDKVEFMCELVSFLSPTDLRLLGNCIEGSVRCYNNQMRPVEKTSNCSDPTAGLPQFVCSPPPPPQQVYYFPGIADAALYQTRNAVNSVFVQQHPPGLPPLLGGMQNIMYPPDANFHHSTSCTTPSSSVSVANKDVNNVPVVLSSSVNGISNNIPSDRQQLDSKPNTARGSSGNINQSNTTSPEPEPGSNHISTTAANPAHPTSVTVPQSVPPVPQEPELFLKSVLDLTSYIYTLMAVCSSTNRKSAAKISDYVQNVILREKSQILERIPDELDKITVLQEIGKIVAAMTHHPAITLDDKMKYAALRDGLRAQIETLFRQYYTTQKQLEQSNLVVPSSQAVGDENDTDSDHESEEEFEPLSGVMGSRFESNPVQPSPSVPGTFFIIRFIGRQIEKNDNLFSLEIHWSDGDRTFAQRSRDQLKALQHRLLDEFGQQRSEKYLHQGCTTSYSSFDDDNKKLSASTSTMETFAPNGERIVPRLARDATPAQYVQYINELSDLPARMMLSAVICEEFNGTRAKTEDETREASDGLIYSRWKNPRAKSPVRYFKRNATGSIDPIELPVNMQPFLYSNIPQTQVQTLFPSCSNCGGPHAPKHCEKQTLLSKKGDHRTRSDGEGSQQNGGTSSSNSYAPIHANLPHAVYIENPQAMLGSNHFNHHQQQHIIQNTIFHNGGQFRANGTYEPQIPIAYYHAQGTVQNSNNTGGVNGNSGGGNQNSNF

Target ORF information:

RefSeq Version	NM_059964.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Germline survival defective-1 (gls-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059964.4

ORF Insert Sequence:

ATGCGATGTT TGATATCTTA TTTATTTCAT TCCTTCCTTA TTTTTTTAAA ATTTATCCGT 
TCAGACGTCA CAGCTTTAAC TCTTCAAGAA AAGCGTAAGA AGTCTCGGTT AACTGGTATT 
TTGATGAAAA GTATGGCCAA TAAAAAGAAC CATCAGCAAA AGAAAAGTAC AGACGGGTCA 
ACGATGAACG GGAATAACGC AACAGCAACT GCCGCTGCAA CAACTCAGAA TTCATCACAA 
ATTGGACAAA ATTCCAATTC CAGTCATAGC GTCACCAACG ATAACACTTC CAGCAATAAC 
GCTTCGACGT CTACATCGTC CATCACCTCA ACTACCACTA CGACCACTAC AGTACCTTCT 
TCACAGCAGA GTCAATCTCA AAATCAATAT TCACATCAAA GACTTTCATC GACTTCTTCA 
ACGTCACAGC AAACTGGAAT TTCTAAATTT CCTGCCAAAA AGGCTGGGCA CAACGAGCTT 
GAGAAGACAA GCAACACTGC TAATTCACAA TCGGGTGCTT TCAAAGGAAC CACAAATAAA 
GATCGGCCGA AGGAAAAGGA GAAAAACACT CACAGTGGTA ATAGGCAAAG TCAGGTTAAC 
CATAATCATC ATCATTCAAA CCATGTTAGC GGAAATCAGC AGAACCGAAA GAATAAGAAT 
CGTGATCATT CGAACCAAAG TTATCGAGGT GGCTACACAC ATAACCACAA TAATTATCAC 
TCGTTGGAAA ATGCTAAATC TTCTGGATTT CTGTCAAATT CTTCCCTATC CTCTGCGGGA 
CAAATATCAG CGTCTTCTGC TCCTCCAGTG AGCACAACTC CGACTGCTAT TCCCATCTTT 
CAAGAATCCA AAATAGTTCA AACAGATCCA CCAGTCGAAG AAGCAAAAAA GAAGAAGGAA 
AAACCAAAAA TCAAGCGTGA TGAAGAGCCG ATGCCATATA AGAGCACTGA TCCGAAAAAT 
ATGGATTCTG TGATGGCATT CAAAGAGCAC GATGAATGGG ATAAGGTGGA GTTTATGTGT 
GAACTTGTTT CTTTCCTGTC TCCAACCGAT CTTCGCCTGC TTGGAAATTG TATCGAAGGA 
TCTGTTCGTT GCTATAACAA TCAAATGCGG CCAGTAGAAA AGACTTCAAA CTGTTCGGAT 
CCAACAGCTG GTTTACCTCA ATTCGTTTGC TCTCCTCCAC CTCCACCGCA ACAAGTTTAT 
TATTTTCCTG GTATTGCGGA TGCTGCCCTG TATCAAACAA GAAATGCAGT TAACTCGGTT 
TTTGTACAGC AACATCCACC TGGTCTTCCA CCGTTATTAG GCGGAATGCA GAATATTATG 
TATCCACCTG ATGCTAACTT TCATCATTCA ACCTCTTGTA CGACACCTTC ATCATCAGTT 
TCCGTTGCAA ATAAGGATGT TAACAATGTA CCAGTTGTGC TATCAAGTAG TGTGAACGGA 
ATAAGCAATA ATATTCCATC AGATAGACAA CAGCTAGATT CCAAACCAAA TACTGCAAGA 
GGTTCGAGCG GCAATATCAA CCAATCTAAT ACAACATCAC CAGAACCGGA GCCCGGTTCT 
AATCACATCT CCACCACAGC AGCAAACCCT GCACATCCAA CTTCAGTGAC TGTACCACAG 
TCGGTACCAC CGGTCCCTCA AGAACCAGAA TTGTTTCTCA AATCTGTTCT CGATCTTACC 
AGTTACATAT ACACTCTCAT GGCAGTTTGT TCATCAACAA ATAGAAAATC AGCTGCTAAG 
ATCTCTGACT ATGTTCAAAA TGTTATTCTG AGAGAAAAGA GCCAAATACT CGAACGTATT 
CCGGATGAAC TTGATAAAAT TACCGTTTTA CAAGAAATCG GTAAAATTGT CGCAGCCATG 
ACTCATCACC CAGCAATTAC TCTTGATGAC AAAATGAAAT ATGCGGCTCT GCGGGACGGA 
TTACGTGCTC AAATAGAAAC ATTGTTTAGA CAATACTACA CAACTCAGAA ACAGTTGGAG 
CAAAGCAATC TTGTTGTACC ATCATCACAA GCTGTTGGCG ATGAGAATGA TACTGATTCC 
GACCATGAAT CAGAGGAAGA ATTTGAGCCA CTATCAGGTG TTATGGGTTC GCGTTTCGAG 
AGTAATCCAG TTCAGCCGTC GCCATCAGTT CCGGGAACAT TCTTTATCAT TCGATTTATT 
GGACGACAAA TTGAGAAGAA CGATAATCTT TTCAGCCTGG AGATTCATTG GAGTGATGGT 
GACAGAACAT TTGCTCAAAG ATCTCGTGAT CAATTGAAAG CTCTACAGCA TCGTCTTCTT 
GATGAGTTCG GACAACAGAG AAGTGAAAAA TATCTTCATC AAGGATGCAC TACATCCTAC 
TCTTCATTTG ACGATGATAA CAAAAAGCTT TCAGCGAGCA CATCTACAAT GGAGACGTTT 
GCTCCAAACG GAGAGCGTAT TGTTCCACGT CTTGCTCGTG ATGCGACTCC AGCTCAATAT 
GTTCAATACA TCAATGAACT TTCTGACTTG CCAGCTAGAA TGATGCTGTC AGCTGTAATA 
TGTGAAGAGT TCAACGGTAC CAGAGCGAAA ACAGAAGATG AAACTCGCGA AGCATCGGAT 
GGATTAATAT ATTCTCGATG GAAGAATCCA CGTGCCAAAT CTCCAGTTCG TTACTTCAAA 
CGAAATGCAA CAGGAAGCAT TGACCCGATT GAATTGCCTG TCAATATGCA ACCATTCCTT 
TATTCAAATA TCCCGCAAAC ACAAGTGCAG ACGCTGTTCC CAAGTTGCTC TAATTGTGGC 
GGTCCTCACG CTCCGAAACA CTGTGAAAAA CAAACATTGT TATCGAAGAA AGGTGATCAT 
AGAACTCGTT CAGACGGGGA AGGTTCTCAG CAAAATGGTG GAACTTCCTC TTCCAATTCA 
TACGCTCCAA TTCATGCAAA CCTCCCCCAT GCAGTATACA TTGAAAATCC GCAAGCAATG 
TTAGGATCAA ATCACTTCAA TCATCATCAA CAACAGCACA TAATTCAGAA TACTATATTC 
CATAATGGTG GACAATTTCG TGCAAACGGA ACATATGAAC CGCAAATTCC GATAGCATAC 
TATCATGCCC AGGGAACTGT ACAAAACTCA AATAATACTG GTGGAGTCAA CGGAAACAGT 
GGGGGCGGGA ACCAGAATTC GAATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gls-1 ( NM_182082.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_871882.2 Caenorhabditis elegans Germline survival defective-1 (gls-1), partial mRNA.

Price & Availability↑

CloneID	OCa335348
Clone ID Related Accession (Same CDS sequence)	NM_182082.5 , NM_182082.6
Accession Version	NM_182082.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Germline survival defective-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_182082.4. COMPLETENESS: incomplete on both ends.

ATGCGATGTT TGATATCTTA TTTATTTCAT TCCTTCCTTA TTTTTTTAAA ATTTATCCGT 
TCAGACGTCA CAGCTTTAAC TCTTCAAGAA AAGCGTAAGA AGTCTCGGTT AACTGGTATT 
TTGATGAAAA GTATGGCCAA TAAAAAGAAC CATCAGCAAA AGAAAAGTAC AGACGGGTCA 
ACGATGAACG GGAATAACGC AACAGCAACT GCCGCTGCAA CAACTCAGAA TTCATCACAA 
ATTGGACAAA ATTCCAATTC CAGTCATAGC GTCACCAACG ATAACACTTC CAGCAATAAC 
GCTTCGACGT CTACATCGTC CATCACCTCA ACTACCACTA CGACCACTAC AGTACCTTCT 
TCACAGCAGA GTCAATCTCA AAATCAATAT TCACATCAAA GACTTTCATC GACTTCTTCA 
ACGTCACAGC AAACTGGAAT TTCTAAATTT CCTGCCAAAA AGGCTGGGCA CAACGAGCTT 
GAGAAGACAA GCAACACTGC TAATTCACAA TCGGGTGCTT TCAAAGGAAC CACAAATAAA 
GATCGGCCGA AGGAAAAGGA GAAAAACACT CACAGTGGTA ATAGGCAAAG TCAGGTTAAC 
CATAATCATC ATCATTCAAA CCATGTTAGC GGAAATCAGC AGAACCGAAA GAATAAGAAT 
CGTGATCATT CGAACCAAAG TTATCGAGGT GGCTACACAC ATAACCACAA TAATTATCAC 
TCGTTGGAAA ATGCTAAATC TTCTGGATTT CTGTCAAATT CTTCCCTATC CTCTGCGGGA 
CAAATATCAG CGTCTTCTGC TCCTCCAGTG AGCACAACTC CGACTGCTAT TCCCATCTTT 
CAAGAATCCA AAATAGTTCA AACAGATCCA CCAGTCGAAG AAGCAAAAAA GAAGAAGGAA 
AAACCAAAAA TCAAGCGTGA TGAAGAGCCG ATGCCATATA AGAGCACTGA TCCGAAAAAT 
ATGGATTCTG TGATGGCATT CAAAGAGCAC GATGAATGGG ATAAGGTGGA GTTTATGTGT 
GAACTTGTTT CTTTCCTGTC TCCAACCGAT CTTCGCCTGC TTGGAAATTG TATCGAAGGA 
TCTGTTCGTT GCTATAACAA TCAAATGCGG CCAGTAGAAA AGACTTCAAA CTGTTCGGAT 
CCAACAGCTG GTTTACCTCA ATTCGTTTGC TCTCCTCCAC CTCCACCGCA ACAAGTTTAT 
TATTTTCCTG GTATTGCGGA TGCTGCCCTG TATCAAACAA GAAATGCAGT TAACTCGGTT 
TTTGTACAGC AACATCCACC TGGTCTTCCA CCGTTATTAG GCGGAATGCA GAATATTATG 
TATCCACCTG ATGCTAACTT TCATCATTCA ACCTCTTGTA CGACACCTTC ATCATCAGTT 
TCCGTTGCAA ATAAGGATGT TAACAATGTA CCAGTTGTGC TATCAAGTAG TGTGAACGGA 
ATAAGCAATA ATATTCCATC AGATAGACAA CAGCTAGATT CCAAACCAAA TACTGCAAGA 
GGTTCGAGCG GCAATATCAA CCAATCTAAT ACAACATCAC CAGAACCGGA GCCCGGTTCT 
AATCACATCT CCACCACAGC AGCAAACCCT GCACATCCAA CTTCAGTGAC TGTACCACAG 
TCGGTACCAC CGGTCCCTCA AGAACCAGAA TTGTTTCTCA AATCTGTTCT CGATCTTACC 
AGTTACATAT ACACTCTCAT GGCAGTTTGT TCATCAACAA ATAGAAAATC AGCTGCTAAG 
ATCTCTGACT ATGTTCAAAA TGTTATTCTG AGAGAAAAGA GCCAAATACT CGAACGTATT 
CCGGATGAAC TTGATAAAAT TACCGTTTTA CAAGAAATCG GTAAAATTGT CGCAGCCATG 
ACTCATCACC CAGCAATTAC TCTTGATGAC AAAATGAAAT ATGCGGCTCT GCGGGACGGA 
TTACGTGCTC AAATAGAAAC ATTGTTTAGA CAATACTACA CAACTCAGAA ACAGTTGGAG 
CAAAGCAATC TTGTTGTACC ATCATCACAA GCTGTTGGCG ATGAGAATGA TACTGATTCC 
GACCATGAAT CAGAGGAAGA ATTTGAGCCA CTATCAGGTG TTATGGGTTC GCGTTTCGAG 
AGTAATCCAG TTCAGCCGTC GCCATCAGTT CCGGGAACAT TCTTTATCAT TCGATTTATT 
GGACGACAAA TTGAGAAGAA CGATAATCTT TTCAGCCTGG AGATTCATTG GAGTGATGGT 
GACAGAACAT TTGCTCAAAG ATCTCGTGAT CAATTGAAAG CTCTACAGCA TCGTCTTCTT 
GATGAGTTCG GACAACAGAG AAGTGAAAAA TATCTTCATC AAGGATGCAC TACATCCTAC 
TCTTCATTTG ACGATGATAA CAAAAAGCTT TCAGCGAGCA CATCTACAAT GGAGACGTTT 
GCTCCAAACG GAGAGCGTAT TGTTCCACGT CTTGCTCGTG ATGCGACTCC AGCTCAATAT 
GTTCAATACA TCAATGAACT TTCTGACTTG CCAGCTAGAA TGATGCTGTC AGCTGTAATA 
TGTGAAGAGT TCAACGGTAC CAGAGCGAAA ACAGAAGATC TATTGCAGGA AACTCGCGAA 
GCATCGGATG GATTAATATA TTCTCGATGG AAGAATCCAC GTGCCAAATC TCCAGTTCGT 
TACTTCAAAC GAAATGCAAC AGGAAGCATT GACCCGATTG AATTGCCTGT CAATATGCAA 
CCATTCCTTT ATTCAAATAT CCCGCAAACA CAAGTGCAGA CGCTGTTCCC AAGTTGCTCT 
AATTGTGGCG GTCCTCACGC TCCGAAACAC TGTGAAAAAC AAACATTGTT ATCGAAGAAA 
GGTGATCATA GAACTCGTTC AGACGGGGAA GGTTCTCAGC AAAATGGTGG AACTTCCTCT 
TCCAATTCAT ACGCTCCAAT TCATGCAAAC CTCCCCCATG CAGTATACAT TGAAAATCCG 
CAAGCAATGT TAGGATCAAA TCACTTCAAT CATCATCAAC AACAGCACAT AATTCAGAAT 
ACTATATTCC ATAATGGTGG ACAATTTCGT GCAAACGGAA CATATGAACC GCAAATTCCG 
ATAGCATACT ATCATGCCCA GGGAACTGTA CAAAACTCAA ATAATACTGG TGGAGTCAAC 
GGAAACAGTG GGGGCGGGAA CCAGAATTCG AATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_871882.2
CDS	1..3159
Translation	MRCLISYLFHSFLIFLKFIRSDVTALTLQEKRKKSRLTGILMKSMANKKNHQQKKSTDGSTMNGNNATATAAATTQNSSQIGQNSNSSHSVTNDNTSSNNASTSTSSITSTTTTTTTVPSSQQSQSQNQYSHQRLSSTSSTSQQTGISKFPAKKAGHNELEKTSNTANSQSGAFKGTTNKDRPKEKEKNTHSGNRQSQVNHNHHHSNHVSGNQQNRKNKNRDHSNQSYRGGYTHNHNNYHSLENAKSSGFLSNSSLSSAGQISASSAPPVSTTPTAIPIFQESKIVQTDPPVEEAKKKKEKPKIKRDEEPMPYKSTDPKNMDSVMAFKEHDEWDKVEFMCELVSFLSPTDLRLLGNCIEGSVRCYNNQMRPVEKTSNCSDPTAGLPQFVCSPPPPPQQVYYFPGIADAALYQTRNAVNSVFVQQHPPGLPPLLGGMQNIMYPPDANFHHSTSCTTPSSSVSVANKDVNNVPVVLSSSVNGISNNIPSDRQQLDSKPNTARGSSGNINQSNTTSPEPEPGSNHISTTAANPAHPTSVTVPQSVPPVPQEPELFLKSVLDLTSYIYTLMAVCSSTNRKSAAKISDYVQNVILREKSQILERIPDELDKITVLQEIGKIVAAMTHHPAITLDDKMKYAALRDGLRAQIETLFRQYYTTQKQLEQSNLVVPSSQAVGDENDTDSDHESEEEFEPLSGVMGSRFESNPVQPSPSVPGTFFIIRFIGRQIEKNDNLFSLEIHWSDGDRTFAQRSRDQLKALQHRLLDEFGQQRSEKYLHQGCTTSYSSFDDDNKKLSASTSTMETFAPNGERIVPRLARDATPAQYVQYINELSDLPARMMLSAVICEEFNGTRAKTEDLLQETREASDGLIYSRWKNPRAKSPVRYFKRNATGSIDPIELPVNMQPFLYSNIPQTQVQTLFPSCSNCGGPHAPKHCEKQTLLSKKGDHRTRSDGEGSQQNGGTSSSNSYAPIHANLPHAVYIENPQAMLGSNHFNHHQQQHIIQNTIFHNGGQFRANGTYEPQIPIAYYHAQGTVQNSNNTGGVNGNSGGGNQNSNF

Target ORF information:

RefSeq Version	NM_182082.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Germline survival defective-1 (gls-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182082.5

ORF Insert Sequence:

ATGCGATGTT TGATATCTTA TTTATTTCAT TCCTTCCTTA TTTTTTTAAA ATTTATCCGT 
TCAGACGTCA CAGCTTTAAC TCTTCAAGAA AAGCGTAAGA AGTCTCGGTT AACTGGTATT 
TTGATGAAAA GTATGGCCAA TAAAAAGAAC CATCAGCAAA AGAAAAGTAC AGACGGGTCA 
ACGATGAACG GGAATAACGC AACAGCAACT GCCGCTGCAA CAACTCAGAA TTCATCACAA 
ATTGGACAAA ATTCCAATTC CAGTCATAGC GTCACCAACG ATAACACTTC CAGCAATAAC 
GCTTCGACGT CTACATCGTC CATCACCTCA ACTACCACTA CGACCACTAC AGTACCTTCT 
TCACAGCAGA GTCAATCTCA AAATCAATAT TCACATCAAA GACTTTCATC GACTTCTTCA 
ACGTCACAGC AAACTGGAAT TTCTAAATTT CCTGCCAAAA AGGCTGGGCA CAACGAGCTT 
GAGAAGACAA GCAACACTGC TAATTCACAA TCGGGTGCTT TCAAAGGAAC CACAAATAAA 
GATCGGCCGA AGGAAAAGGA GAAAAACACT CACAGTGGTA ATAGGCAAAG TCAGGTTAAC 
CATAATCATC ATCATTCAAA CCATGTTAGC GGAAATCAGC AGAACCGAAA GAATAAGAAT 
CGTGATCATT CGAACCAAAG TTATCGAGGT GGCTACACAC ATAACCACAA TAATTATCAC 
TCGTTGGAAA ATGCTAAATC TTCTGGATTT CTGTCAAATT CTTCCCTATC CTCTGCGGGA 
CAAATATCAG CGTCTTCTGC TCCTCCAGTG AGCACAACTC CGACTGCTAT TCCCATCTTT 
CAAGAATCCA AAATAGTTCA AACAGATCCA CCAGTCGAAG AAGCAAAAAA GAAGAAGGAA 
AAACCAAAAA TCAAGCGTGA TGAAGAGCCG ATGCCATATA AGAGCACTGA TCCGAAAAAT 
ATGGATTCTG TGATGGCATT CAAAGAGCAC GATGAATGGG ATAAGGTGGA GTTTATGTGT 
GAACTTGTTT CTTTCCTGTC TCCAACCGAT CTTCGCCTGC TTGGAAATTG TATCGAAGGA 
TCTGTTCGTT GCTATAACAA TCAAATGCGG CCAGTAGAAA AGACTTCAAA CTGTTCGGAT 
CCAACAGCTG GTTTACCTCA ATTCGTTTGC TCTCCTCCAC CTCCACCGCA ACAAGTTTAT 
TATTTTCCTG GTATTGCGGA TGCTGCCCTG TATCAAACAA GAAATGCAGT TAACTCGGTT 
TTTGTACAGC AACATCCACC TGGTCTTCCA CCGTTATTAG GCGGAATGCA GAATATTATG 
TATCCACCTG ATGCTAACTT TCATCATTCA ACCTCTTGTA CGACACCTTC ATCATCAGTT 
TCCGTTGCAA ATAAGGATGT TAACAATGTA CCAGTTGTGC TATCAAGTAG TGTGAACGGA 
ATAAGCAATA ATATTCCATC AGATAGACAA CAGCTAGATT CCAAACCAAA TACTGCAAGA 
GGTTCGAGCG GCAATATCAA CCAATCTAAT ACAACATCAC CAGAACCGGA GCCCGGTTCT 
AATCACATCT CCACCACAGC AGCAAACCCT GCACATCCAA CTTCAGTGAC TGTACCACAG 
TCGGTACCAC CGGTCCCTCA AGAACCAGAA TTGTTTCTCA AATCTGTTCT CGATCTTACC 
AGTTACATAT ACACTCTCAT GGCAGTTTGT TCATCAACAA ATAGAAAATC AGCTGCTAAG 
ATCTCTGACT ATGTTCAAAA TGTTATTCTG AGAGAAAAGA GCCAAATACT CGAACGTATT 
CCGGATGAAC TTGATAAAAT TACCGTTTTA CAAGAAATCG GTAAAATTGT CGCAGCCATG 
ACTCATCACC CAGCAATTAC TCTTGATGAC AAAATGAAAT ATGCGGCTCT GCGGGACGGA 
TTACGTGCTC AAATAGAAAC ATTGTTTAGA CAATACTACA CAACTCAGAA ACAGTTGGAG 
CAAAGCAATC TTGTTGTACC ATCATCACAA GCTGTTGGCG ATGAGAATGA TACTGATTCC 
GACCATGAAT CAGAGGAAGA ATTTGAGCCA CTATCAGGTG TTATGGGTTC GCGTTTCGAG 
AGTAATCCAG TTCAGCCGTC GCCATCAGTT CCGGGAACAT TCTTTATCAT TCGATTTATT 
GGACGACAAA TTGAGAAGAA CGATAATCTT TTCAGCCTGG AGATTCATTG GAGTGATGGT 
GACAGAACAT TTGCTCAAAG ATCTCGTGAT CAATTGAAAG CTCTACAGCA TCGTCTTCTT 
GATGAGTTCG GACAACAGAG AAGTGAAAAA TATCTTCATC AAGGATGCAC TACATCCTAC 
TCTTCATTTG ACGATGATAA CAAAAAGCTT TCAGCGAGCA CATCTACAAT GGAGACGTTT 
GCTCCAAACG GAGAGCGTAT TGTTCCACGT CTTGCTCGTG ATGCGACTCC AGCTCAATAT 
GTTCAATACA TCAATGAACT TTCTGACTTG CCAGCTAGAA TGATGCTGTC AGCTGTAATA 
TGTGAAGAGT TCAACGGTAC CAGAGCGAAA ACAGAAGATC TATTGCAGGA AACTCGCGAA 
GCATCGGATG GATTAATATA TTCTCGATGG AAGAATCCAC GTGCCAAATC TCCAGTTCGT 
TACTTCAAAC GAAATGCAAC AGGAAGCATT GACCCGATTG AATTGCCTGT CAATATGCAA 
CCATTCCTTT ATTCAAATAT CCCGCAAACA CAAGTGCAGA CGCTGTTCCC AAGTTGCTCT 
AATTGTGGCG GTCCTCACGC TCCGAAACAC TGTGAAAAAC AAACATTGTT ATCGAAGAAA 
GGTGATCATA GAACTCGTTC AGACGGGGAA GGTTCTCAGC AAAATGGTGG AACTTCCTCT 
TCCAATTCAT ACGCTCCAAT TCATGCAAAC CTCCCCCATG CAGTATACAT TGAAAATCCG 
CAAGCAATGT TAGGATCAAA TCACTTCAAT CATCATCAAC AACAGCACAT AATTCAGAAT 
ACTATATTCC ATAATGGTGG ACAATTTCGT GCAAACGGAA CATATGAACC GCAAATTCCG 
ATAGCATACT ATCATGCCCA GGGAACTGTA CAAAACTCAA ATAATACTGG TGGAGTCAAC 
GGAAACAGTG GGGGCGGGAA CCAGAATTCG AATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gls-1 ( NM_182082.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_871882.2 Caenorhabditis elegans Germline survival defective-1 (gls-1), partial mRNA.

Price & Availability↑

CloneID	OCa335348
Clone ID Related Accession (Same CDS sequence)	NM_182082.5 , NM_182082.6
Accession Version	NM_182082.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Germline survival defective-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_182082.5. COMPLETENESS: incomplete on the 5' end.

ATGCGATGTT TGATATCTTA TTTATTTCAT TCCTTCCTTA TTTTTTTAAA ATTTATCCGT 
TCAGACGTCA CAGCTTTAAC TCTTCAAGAA AAGCGTAAGA AGTCTCGGTT AACTGGTATT 
TTGATGAAAA GTATGGCCAA TAAAAAGAAC CATCAGCAAA AGAAAAGTAC AGACGGGTCA 
ACGATGAACG GGAATAACGC AACAGCAACT GCCGCTGCAA CAACTCAGAA TTCATCACAA 
ATTGGACAAA ATTCCAATTC CAGTCATAGC GTCACCAACG ATAACACTTC CAGCAATAAC 
GCTTCGACGT CTACATCGTC CATCACCTCA ACTACCACTA CGACCACTAC AGTACCTTCT 
TCACAGCAGA GTCAATCTCA AAATCAATAT TCACATCAAA GACTTTCATC GACTTCTTCA 
ACGTCACAGC AAACTGGAAT TTCTAAATTT CCTGCCAAAA AGGCTGGGCA CAACGAGCTT 
GAGAAGACAA GCAACACTGC TAATTCACAA TCGGGTGCTT TCAAAGGAAC CACAAATAAA 
GATCGGCCGA AGGAAAAGGA GAAAAACACT CACAGTGGTA ATAGGCAAAG TCAGGTTAAC 
CATAATCATC ATCATTCAAA CCATGTTAGC GGAAATCAGC AGAACCGAAA GAATAAGAAT 
CGTGATCATT CGAACCAAAG TTATCGAGGT GGCTACACAC ATAACCACAA TAATTATCAC 
TCGTTGGAAA ATGCTAAATC TTCTGGATTT CTGTCAAATT CTTCCCTATC CTCTGCGGGA 
CAAATATCAG CGTCTTCTGC TCCTCCAGTG AGCACAACTC CGACTGCTAT TCCCATCTTT 
CAAGAATCCA AAATAGTTCA AACAGATCCA CCAGTCGAAG AAGCAAAAAA GAAGAAGGAA 
AAACCAAAAA TCAAGCGTGA TGAAGAGCCG ATGCCATATA AGAGCACTGA TCCGAAAAAT 
ATGGATTCTG TGATGGCATT CAAAGAGCAC GATGAATGGG ATAAGGTGGA GTTTATGTGT 
GAACTTGTTT CTTTCCTGTC TCCAACCGAT CTTCGCCTGC TTGGAAATTG TATCGAAGGA 
TCTGTTCGTT GCTATAACAA TCAAATGCGG CCAGTAGAAA AGACTTCAAA CTGTTCGGAT 
CCAACAGCTG GTTTACCTCA ATTCGTTTGC TCTCCTCCAC CTCCACCGCA ACAAGTTTAT 
TATTTTCCTG GTATTGCGGA TGCTGCCCTG TATCAAACAA GAAATGCAGT TAACTCGGTT 
TTTGTACAGC AACATCCACC TGGTCTTCCA CCGTTATTAG GCGGAATGCA GAATATTATG 
TATCCACCTG ATGCTAACTT TCATCATTCA ACCTCTTGTA CGACACCTTC ATCATCAGTT 
TCCGTTGCAA ATAAGGATGT TAACAATGTA CCAGTTGTGC TATCAAGTAG TGTGAACGGA 
ATAAGCAATA ATATTCCATC AGATAGACAA CAGCTAGATT CCAAACCAAA TACTGCAAGA 
GGTTCGAGCG GCAATATCAA CCAATCTAAT ACAACATCAC CAGAACCGGA GCCCGGTTCT 
AATCACATCT CCACCACAGC AGCAAACCCT GCACATCCAA CTTCAGTGAC TGTACCACAG 
TCGGTACCAC CGGTCCCTCA AGAACCAGAA TTGTTTCTCA AATCTGTTCT CGATCTTACC 
AGTTACATAT ACACTCTCAT GGCAGTTTGT TCATCAACAA ATAGAAAATC AGCTGCTAAG 
ATCTCTGACT ATGTTCAAAA TGTTATTCTG AGAGAAAAGA GCCAAATACT CGAACGTATT 
CCGGATGAAC TTGATAAAAT TACCGTTTTA CAAGAAATCG GTAAAATTGT CGCAGCCATG 
ACTCATCACC CAGCAATTAC TCTTGATGAC AAAATGAAAT ATGCGGCTCT GCGGGACGGA 
TTACGTGCTC AAATAGAAAC ATTGTTTAGA CAATACTACA CAACTCAGAA ACAGTTGGAG 
CAAAGCAATC TTGTTGTACC ATCATCACAA GCTGTTGGCG ATGAGAATGA TACTGATTCC 
GACCATGAAT CAGAGGAAGA ATTTGAGCCA CTATCAGGTG TTATGGGTTC GCGTTTCGAG 
AGTAATCCAG TTCAGCCGTC GCCATCAGTT CCGGGAACAT TCTTTATCAT TCGATTTATT 
GGACGACAAA TTGAGAAGAA CGATAATCTT TTCAGCCTGG AGATTCATTG GAGTGATGGT 
GACAGAACAT TTGCTCAAAG ATCTCGTGAT CAATTGAAAG CTCTACAGCA TCGTCTTCTT 
GATGAGTTCG GACAACAGAG AAGTGAAAAA TATCTTCATC AAGGATGCAC TACATCCTAC 
TCTTCATTTG ACGATGATAA CAAAAAGCTT TCAGCGAGCA CATCTACAAT GGAGACGTTT 
GCTCCAAACG GAGAGCGTAT TGTTCCACGT CTTGCTCGTG ATGCGACTCC AGCTCAATAT 
GTTCAATACA TCAATGAACT TTCTGACTTG CCAGCTAGAA TGATGCTGTC AGCTGTAATA 
TGTGAAGAGT TCAACGGTAC CAGAGCGAAA ACAGAAGATC TATTGCAGGA AACTCGCGAA 
GCATCGGATG GATTAATATA TTCTCGATGG AAGAATCCAC GTGCCAAATC TCCAGTTCGT 
TACTTCAAAC GAAATGCAAC AGGAAGCATT GACCCGATTG AATTGCCTGT CAATATGCAA 
CCATTCCTTT ATTCAAATAT CCCGCAAACA CAAGTGCAGA CGCTGTTCCC AAGTTGCTCT 
AATTGTGGCG GTCCTCACGC TCCGAAACAC TGTGAAAAAC AAACATTGTT ATCGAAGAAA 
GGTGATCATA GAACTCGTTC AGACGGGGAA GGTTCTCAGC AAAATGGTGG AACTTCCTCT 
TCCAATTCAT ACGCTCCAAT TCATGCAAAC CTCCCCCATG CAGTATACAT TGAAAATCCG 
CAAGCAATGT TAGGATCAAA TCACTTCAAT CATCATCAAC AACAGCACAT AATTCAGAAT 
ACTATATTCC ATAATGGTGG ACAATTTCGT GCAAACGGAA CATATGAACC GCAAATTCCG 
ATAGCATACT ATCATGCCCA GGGAACTGTA CAAAACTCAA ATAATACTGG TGGAGTCAAC 
GGAAACAGTG GGGGCGGGAA CCAGAATTCG AATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_871882.2
CDS	1..3159
Translation	MRCLISYLFHSFLIFLKFIRSDVTALTLQEKRKKSRLTGILMKSMANKKNHQQKKSTDGSTMNGNNATATAAATTQNSSQIGQNSNSSHSVTNDNTSSNNASTSTSSITSTTTTTTTVPSSQQSQSQNQYSHQRLSSTSSTSQQTGISKFPAKKAGHNELEKTSNTANSQSGAFKGTTNKDRPKEKEKNTHSGNRQSQVNHNHHHSNHVSGNQQNRKNKNRDHSNQSYRGGYTHNHNNYHSLENAKSSGFLSNSSLSSAGQISASSAPPVSTTPTAIPIFQESKIVQTDPPVEEAKKKKEKPKIKRDEEPMPYKSTDPKNMDSVMAFKEHDEWDKVEFMCELVSFLSPTDLRLLGNCIEGSVRCYNNQMRPVEKTSNCSDPTAGLPQFVCSPPPPPQQVYYFPGIADAALYQTRNAVNSVFVQQHPPGLPPLLGGMQNIMYPPDANFHHSTSCTTPSSSVSVANKDVNNVPVVLSSSVNGISNNIPSDRQQLDSKPNTARGSSGNINQSNTTSPEPEPGSNHISTTAANPAHPTSVTVPQSVPPVPQEPELFLKSVLDLTSYIYTLMAVCSSTNRKSAAKISDYVQNVILREKSQILERIPDELDKITVLQEIGKIVAAMTHHPAITLDDKMKYAALRDGLRAQIETLFRQYYTTQKQLEQSNLVVPSSQAVGDENDTDSDHESEEEFEPLSGVMGSRFESNPVQPSPSVPGTFFIIRFIGRQIEKNDNLFSLEIHWSDGDRTFAQRSRDQLKALQHRLLDEFGQQRSEKYLHQGCTTSYSSFDDDNKKLSASTSTMETFAPNGERIVPRLARDATPAQYVQYINELSDLPARMMLSAVICEEFNGTRAKTEDLLQETREASDGLIYSRWKNPRAKSPVRYFKRNATGSIDPIELPVNMQPFLYSNIPQTQVQTLFPSCSNCGGPHAPKHCEKQTLLSKKGDHRTRSDGEGSQQNGGTSSSNSYAPIHANLPHAVYIENPQAMLGSNHFNHHQQQHIIQNTIFHNGGQFRANGTYEPQIPIAYYHAQGTVQNSNNTGGVNGNSGGGNQNSNF

Target ORF information:

RefSeq Version	NM_182082.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Germline survival defective-1 (gls-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182082.6

ORF Insert Sequence:

ATGCGATGTT TGATATCTTA TTTATTTCAT TCCTTCCTTA TTTTTTTAAA ATTTATCCGT 
TCAGACGTCA CAGCTTTAAC TCTTCAAGAA AAGCGTAAGA AGTCTCGGTT AACTGGTATT 
TTGATGAAAA GTATGGCCAA TAAAAAGAAC CATCAGCAAA AGAAAAGTAC AGACGGGTCA 
ACGATGAACG GGAATAACGC AACAGCAACT GCCGCTGCAA CAACTCAGAA TTCATCACAA 
ATTGGACAAA ATTCCAATTC CAGTCATAGC GTCACCAACG ATAACACTTC CAGCAATAAC 
GCTTCGACGT CTACATCGTC CATCACCTCA ACTACCACTA CGACCACTAC AGTACCTTCT 
TCACAGCAGA GTCAATCTCA AAATCAATAT TCACATCAAA GACTTTCATC GACTTCTTCA 
ACGTCACAGC AAACTGGAAT TTCTAAATTT CCTGCCAAAA AGGCTGGGCA CAACGAGCTT 
GAGAAGACAA GCAACACTGC TAATTCACAA TCGGGTGCTT TCAAAGGAAC CACAAATAAA 
GATCGGCCGA AGGAAAAGGA GAAAAACACT CACAGTGGTA ATAGGCAAAG TCAGGTTAAC 
CATAATCATC ATCATTCAAA CCATGTTAGC GGAAATCAGC AGAACCGAAA GAATAAGAAT 
CGTGATCATT CGAACCAAAG TTATCGAGGT GGCTACACAC ATAACCACAA TAATTATCAC 
TCGTTGGAAA ATGCTAAATC TTCTGGATTT CTGTCAAATT CTTCCCTATC CTCTGCGGGA 
CAAATATCAG CGTCTTCTGC TCCTCCAGTG AGCACAACTC CGACTGCTAT TCCCATCTTT 
CAAGAATCCA AAATAGTTCA AACAGATCCA CCAGTCGAAG AAGCAAAAAA GAAGAAGGAA 
AAACCAAAAA TCAAGCGTGA TGAAGAGCCG ATGCCATATA AGAGCACTGA TCCGAAAAAT 
ATGGATTCTG TGATGGCATT CAAAGAGCAC GATGAATGGG ATAAGGTGGA GTTTATGTGT 
GAACTTGTTT CTTTCCTGTC TCCAACCGAT CTTCGCCTGC TTGGAAATTG TATCGAAGGA 
TCTGTTCGTT GCTATAACAA TCAAATGCGG CCAGTAGAAA AGACTTCAAA CTGTTCGGAT 
CCAACAGCTG GTTTACCTCA ATTCGTTTGC TCTCCTCCAC CTCCACCGCA ACAAGTTTAT 
TATTTTCCTG GTATTGCGGA TGCTGCCCTG TATCAAACAA GAAATGCAGT TAACTCGGTT 
TTTGTACAGC AACATCCACC TGGTCTTCCA CCGTTATTAG GCGGAATGCA GAATATTATG 
TATCCACCTG ATGCTAACTT TCATCATTCA ACCTCTTGTA CGACACCTTC ATCATCAGTT 
TCCGTTGCAA ATAAGGATGT TAACAATGTA CCAGTTGTGC TATCAAGTAG TGTGAACGGA 
ATAAGCAATA ATATTCCATC AGATAGACAA CAGCTAGATT CCAAACCAAA TACTGCAAGA 
GGTTCGAGCG GCAATATCAA CCAATCTAAT ACAACATCAC CAGAACCGGA GCCCGGTTCT 
AATCACATCT CCACCACAGC AGCAAACCCT GCACATCCAA CTTCAGTGAC TGTACCACAG 
TCGGTACCAC CGGTCCCTCA AGAACCAGAA TTGTTTCTCA AATCTGTTCT CGATCTTACC 
AGTTACATAT ACACTCTCAT GGCAGTTTGT TCATCAACAA ATAGAAAATC AGCTGCTAAG 
ATCTCTGACT ATGTTCAAAA TGTTATTCTG AGAGAAAAGA GCCAAATACT CGAACGTATT 
CCGGATGAAC TTGATAAAAT TACCGTTTTA CAAGAAATCG GTAAAATTGT CGCAGCCATG 
ACTCATCACC CAGCAATTAC TCTTGATGAC AAAATGAAAT ATGCGGCTCT GCGGGACGGA 
TTACGTGCTC AAATAGAAAC ATTGTTTAGA CAATACTACA CAACTCAGAA ACAGTTGGAG 
CAAAGCAATC TTGTTGTACC ATCATCACAA GCTGTTGGCG ATGAGAATGA TACTGATTCC 
GACCATGAAT CAGAGGAAGA ATTTGAGCCA CTATCAGGTG TTATGGGTTC GCGTTTCGAG 
AGTAATCCAG TTCAGCCGTC GCCATCAGTT CCGGGAACAT TCTTTATCAT TCGATTTATT 
GGACGACAAA TTGAGAAGAA CGATAATCTT TTCAGCCTGG AGATTCATTG GAGTGATGGT 
GACAGAACAT TTGCTCAAAG ATCTCGTGAT CAATTGAAAG CTCTACAGCA TCGTCTTCTT 
GATGAGTTCG GACAACAGAG AAGTGAAAAA TATCTTCATC AAGGATGCAC TACATCCTAC 
TCTTCATTTG ACGATGATAA CAAAAAGCTT TCAGCGAGCA CATCTACAAT GGAGACGTTT 
GCTCCAAACG GAGAGCGTAT TGTTCCACGT CTTGCTCGTG ATGCGACTCC AGCTCAATAT 
GTTCAATACA TCAATGAACT TTCTGACTTG CCAGCTAGAA TGATGCTGTC AGCTGTAATA 
TGTGAAGAGT TCAACGGTAC CAGAGCGAAA ACAGAAGATC TATTGCAGGA AACTCGCGAA 
GCATCGGATG GATTAATATA TTCTCGATGG AAGAATCCAC GTGCCAAATC TCCAGTTCGT 
TACTTCAAAC GAAATGCAAC AGGAAGCATT GACCCGATTG AATTGCCTGT CAATATGCAA 
CCATTCCTTT ATTCAAATAT CCCGCAAACA CAAGTGCAGA CGCTGTTCCC AAGTTGCTCT 
AATTGTGGCG GTCCTCACGC TCCGAAACAC TGTGAAAAAC AAACATTGTT ATCGAAGAAA 
GGTGATCATA GAACTCGTTC AGACGGGGAA GGTTCTCAGC AAAATGGTGG AACTTCCTCT 
TCCAATTCAT ACGCTCCAAT TCATGCAAAC CTCCCCCATG CAGTATACAT TGAAAATCCG 
CAAGCAATGT TAGGATCAAA TCACTTCAAT CATCATCAAC AACAGCACAT AATTCAGAAT 
ACTATATTCC ATAATGGTGG ACAATTTCGT GCAAACGGAA CATATGAACC GCAAATTCCG 
ATAGCATACT ATCATGCCCA GGGAACTGTA CAAAACTCAA ATAATACTGG TGGAGTCAAC 
GGAAACAGTG GGGGCGGGAA CCAGAATTCG AATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gls-1 ( NM_001263949.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001250878.1 Caenorhabditis elegans Germline survival defective-1 (gls-1), partial mRNA.

Price & Availability↑

CloneID	OCa336569
Clone ID Related Accession (Same CDS sequence)	NM_001263949.1 , NM_001263949.2
Accession Version	NM_001263949.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Germline survival defective-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends.

ATGGCCAATA AAAAGAACCA TCAGCAAAAG AAAAGTACAG ACGGGTCAAC GATGAACGGG 
AATAACGCAA CAGCAACTGC CGCTGCAACA ACTCAGAATT CATCACAAAT TGGACAAAAT 
TCCAATTCCA GTCATAGCGT CACCAACGAT AACACTTCCA GCAATAACGC TTCGACGTCT 
ACATCGTCCA TCACCTCAAC TACCACTACG ACCACTACAG TACCTTCTTC ACAGCAGAGT 
CAATCTCAAA ATCAATATTC ACATCAAAGA CTTTCATCGA CTTCTTCAAC GTCACAGCAA 
ACTGGAATTT CTAAATTTCC TGCCAAAAAG GCTGGGCACA ACGAGCTTGA GAAGACAAGC 
AACACTGCTA ATTCACAATC GGGTGCTTTC AAAGGAACCA CAAATAAAGA TCGGCCGAAG 
GAAAAGGAGA AAAACACTCA CAGTGGTAAT AGGCAAAGTC AGGTTAACCA TAATCATCAT 
CATTCAAACC ATGTTAGCGG AAATCAGCAG AACCGAAAGA ATAAGAATCG TGATCATTCG 
AACCAAAGTT ATCGAGGTGG CTACACACAT AACCACAATA ATTATCACTC GTTGGAAAAT 
GCTAAATCTT CTGGATTTCT GTCAAATTCT TCCCTATCCT CTGCGGGACA AATATCAGCG 
TCTTCTGCTC CTCCAGTGAG CACAACTCCG ACTGCTATTC CCATCTTTCA AGAATCCAAA 
ATAGTTCAAA CAGATCCACC AGTCGAAGAA GCAAAAAAGA AGAAGGAAAA ACCAAAAATC 
AAGCGTGATG AAGAGCCGAT GCCATATAAG AGCACTGATC CGAAAAATAT GGATTCTGTG 
ATGGCATTCA AAGAGCACGA TGAATGGGAT AAGGTGGAGT TTATGTGTGA ACTTGTTTCT 
TTCCTGTCTC CAACCGATCT TCGCCTGCTT GGAAATTGTA TCGAAGGATC TGTTCGTTGC 
TATAACAATC AAATGCGGCC AGTAGAAAAG ACTTCAAACT GTTCGGATCC AACAGCTGGT 
TTACCTCAAT TCGTTTGCTC TCCTCCACCT CCACCGCAAC AAGTTTATTA TTTTCCTGGT 
ATTGCGGATG CTGCCCTGTA TCAAACAAGA AATGCAGTTA ACTCGGTTTT TGTACAGCAA 
CATCCACCTG GTCTTCCACC GTTATTAGGC GGAATGCAGA ATATTATGTA TCCACCTGAT 
GCTAACTTTC ATCATTCAAC CTCTTGTACG ACACCTTCAT CATCAGTTTC CGTTGCAAAT 
AAGGATGTTA ACAATGTACC AGTTGTGCTA TCAAGTAGTG TGAACGGAAT AAGCAATAAT 
ATTCCATCAG ATAGACAACA GCTAGATTCC AAACCAAATA CTGCAAGAGG TTCGAGCGGC 
AATATCAACC AATCTAATAC AACATCACCA GAACCGGAGC CCGGTTCTAA TCACATCTCC 
ACCACAGCAG CAAACCCTGC ACATCCAACT TCAGTGACTG TACCACAGTC GGTACCACCG 
GTCCCTCAAG AACCAGAATT GTTTCTCAAA TCTGTTCTCG ATCTTACCAG TTACATATAC 
ACTCTCATGG CAGTTTGTTC ATCAACAAAT AGAAAATCAG CTGCTAAGAT CTCTGACTAT 
GTTCAAAATG TTATTCTGAG AGAAAAGAGC CAAATACTCG AACGTATTCC GGATGAACTT 
GATAAAATTA CCGTTTTACA AGAAATCGGT AAAATTGTCG CAGCCATGAC TCATCACCCA 
GCAATTACTC TTGATGACAA AATGAAATAT GCGGCTCTGC GGGACGGATT ACGTGCTCAA 
ATAGAAACAT TGTTTAGACA ATACTACACA ACTCAGAAAC AGTTGGAGCA AAGCAATCTT 
GTTGTACCAT CATCACAAGC TGTTGGCGAT GAGAATGATA CTGATTCCGA CCATGAATCA 
GAGGAAGAAT TTGAGCCACT ATCAGGTGTT ATGGGTTCGC GTTTCGAGAG TAATCCAGTT 
CAGCCGTCGC CATCAGTTCC GGGAACATTC TTTATCATTC GATTTATTGG ACGACAAATT 
GAGAAGAACG ATAATCTTTT CAGCCTGGAG ATTCATTGGA GTGATGGTGA CAGAACATTT 
GCTCAAAGAT CTCGTGATCA ATTGAAAGCT CTACAGCATC GTCTTCTTGA TGAGTTCGGA 
CAACAGAGAA GTGAAAAATA TCTTCATCAA GGATGCACTA CATCCTACTC TTCATTTGAC 
GATGATAACA AAAAGCTTTC AGCGAGCACA TCTACAATGG AGACGTTTGC TCCAAACGGA 
GAGCGTATTG TTCCACGTCT TGCTCGTGAT GCGACTCCAG CTCAATATGT TCAATACATC 
AATGAACTTT CTGACTTGCC AGCTAGAATG ATGCTGTCAG CTGTAATATG TGAAGAGTTC 
AACGGTACCA GAGCGAAAAC AGAAGATCTA TTGCAGGAAA CTCGCGAAGC ATCGGATGGA 
TTAATATATT CTCGATGGAA GAATCCACGT GCCAAATCTC CAGTTCGTTA CTTCAAACGA 
AATGCAACAG GAAGCATTGA CCCGATTGAA TTGCCTGTCA ATATGCAACC ATTCCTTTAT 
TCAAATATCC CGCAAACACA AGTGCAGACG CTGTTCCCAA GTTGCTCTAA TTGTGGCGGT 
CCTCACGCTC CGAAACACTG TGAAAAACAA ACATTGTTAT CGAAGAAAGG TGATCATAGA 
ACTCGTTCAG ACGGGGAAGG TTCTCAGCAA AATGGTGGAA CTTCCTCTTC CAATTCATAC 
GCTCCAATTC ATGCAAACCT CCCCCATGCA GTATACATTG AAAATCCGCA AGCAATGTTA 
GGATCAAATC ACTTCAATCA TCATCAACAA CAGCACATAA TTCAGAATAC TATATTCCAT 
AATGGTGGAC AATTTCGTGC AAACGGAACA TATGAACCGC AAATTCCGAT AGCATACTAT 
CATGCCCAGG GAACTGTACA AAACTCAAAT AATACTGGTG GAGTCAACGG AAACAGTGGG 
GGCGGGAACC AGAATTCGAA TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001250878.1
CDS	1..3027
Translation	MANKKNHQQKKSTDGSTMNGNNATATAAATTQNSSQIGQNSNSSHSVTNDNTSSNNASTSTSSITSTTTTTTTVPSSQQSQSQNQYSHQRLSSTSSTSQQTGISKFPAKKAGHNELEKTSNTANSQSGAFKGTTNKDRPKEKEKNTHSGNRQSQVNHNHHHSNHVSGNQQNRKNKNRDHSNQSYRGGYTHNHNNYHSLENAKSSGFLSNSSLSSAGQISASSAPPVSTTPTAIPIFQESKIVQTDPPVEEAKKKKEKPKIKRDEEPMPYKSTDPKNMDSVMAFKEHDEWDKVEFMCELVSFLSPTDLRLLGNCIEGSVRCYNNQMRPVEKTSNCSDPTAGLPQFVCSPPPPPQQVYYFPGIADAALYQTRNAVNSVFVQQHPPGLPPLLGGMQNIMYPPDANFHHSTSCTTPSSSVSVANKDVNNVPVVLSSSVNGISNNIPSDRQQLDSKPNTARGSSGNINQSNTTSPEPEPGSNHISTTAANPAHPTSVTVPQSVPPVPQEPELFLKSVLDLTSYIYTLMAVCSSTNRKSAAKISDYVQNVILREKSQILERIPDELDKITVLQEIGKIVAAMTHHPAITLDDKMKYAALRDGLRAQIETLFRQYYTTQKQLEQSNLVVPSSQAVGDENDTDSDHESEEEFEPLSGVMGSRFESNPVQPSPSVPGTFFIIRFIGRQIEKNDNLFSLEIHWSDGDRTFAQRSRDQLKALQHRLLDEFGQQRSEKYLHQGCTTSYSSFDDDNKKLSASTSTMETFAPNGERIVPRLARDATPAQYVQYINELSDLPARMMLSAVICEEFNGTRAKTEDLLQETREASDGLIYSRWKNPRAKSPVRYFKRNATGSIDPIELPVNMQPFLYSNIPQTQVQTLFPSCSNCGGPHAPKHCEKQTLLSKKGDHRTRSDGEGSQQNGGTSSSNSYAPIHANLPHAVYIENPQAMLGSNHFNHHQQQHIIQNTIFHNGGQFRANGTYEPQIPIAYYHAQGTVQNSNNTGGVNGNSGGGNQNSNF

Target ORF information:

RefSeq Version	NM_001263949.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Germline survival defective-1 (gls-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001263949.1

ORF Insert Sequence:

ATGGCCAATA AAAAGAACCA TCAGCAAAAG AAAAGTACAG ACGGGTCAAC GATGAACGGG 
AATAACGCAA CAGCAACTGC CGCTGCAACA ACTCAGAATT CATCACAAAT TGGACAAAAT 
TCCAATTCCA GTCATAGCGT CACCAACGAT AACACTTCCA GCAATAACGC TTCGACGTCT 
ACATCGTCCA TCACCTCAAC TACCACTACG ACCACTACAG TACCTTCTTC ACAGCAGAGT 
CAATCTCAAA ATCAATATTC ACATCAAAGA CTTTCATCGA CTTCTTCAAC GTCACAGCAA 
ACTGGAATTT CTAAATTTCC TGCCAAAAAG GCTGGGCACA ACGAGCTTGA GAAGACAAGC 
AACACTGCTA ATTCACAATC GGGTGCTTTC AAAGGAACCA CAAATAAAGA TCGGCCGAAG 
GAAAAGGAGA AAAACACTCA CAGTGGTAAT AGGCAAAGTC AGGTTAACCA TAATCATCAT 
CATTCAAACC ATGTTAGCGG AAATCAGCAG AACCGAAAGA ATAAGAATCG TGATCATTCG 
AACCAAAGTT ATCGAGGTGG CTACACACAT AACCACAATA ATTATCACTC GTTGGAAAAT 
GCTAAATCTT CTGGATTTCT GTCAAATTCT TCCCTATCCT CTGCGGGACA AATATCAGCG 
TCTTCTGCTC CTCCAGTGAG CACAACTCCG ACTGCTATTC CCATCTTTCA AGAATCCAAA 
ATAGTTCAAA CAGATCCACC AGTCGAAGAA GCAAAAAAGA AGAAGGAAAA ACCAAAAATC 
AAGCGTGATG AAGAGCCGAT GCCATATAAG AGCACTGATC CGAAAAATAT GGATTCTGTG 
ATGGCATTCA AAGAGCACGA TGAATGGGAT AAGGTGGAGT TTATGTGTGA ACTTGTTTCT 
TTCCTGTCTC CAACCGATCT TCGCCTGCTT GGAAATTGTA TCGAAGGATC TGTTCGTTGC 
TATAACAATC AAATGCGGCC AGTAGAAAAG ACTTCAAACT GTTCGGATCC AACAGCTGGT 
TTACCTCAAT TCGTTTGCTC TCCTCCACCT CCACCGCAAC AAGTTTATTA TTTTCCTGGT 
ATTGCGGATG CTGCCCTGTA TCAAACAAGA AATGCAGTTA ACTCGGTTTT TGTACAGCAA 
CATCCACCTG GTCTTCCACC GTTATTAGGC GGAATGCAGA ATATTATGTA TCCACCTGAT 
GCTAACTTTC ATCATTCAAC CTCTTGTACG ACACCTTCAT CATCAGTTTC CGTTGCAAAT 
AAGGATGTTA ACAATGTACC AGTTGTGCTA TCAAGTAGTG TGAACGGAAT AAGCAATAAT 
ATTCCATCAG ATAGACAACA GCTAGATTCC AAACCAAATA CTGCAAGAGG TTCGAGCGGC 
AATATCAACC AATCTAATAC AACATCACCA GAACCGGAGC CCGGTTCTAA TCACATCTCC 
ACCACAGCAG CAAACCCTGC ACATCCAACT TCAGTGACTG TACCACAGTC GGTACCACCG 
GTCCCTCAAG AACCAGAATT GTTTCTCAAA TCTGTTCTCG ATCTTACCAG TTACATATAC 
ACTCTCATGG CAGTTTGTTC ATCAACAAAT AGAAAATCAG CTGCTAAGAT CTCTGACTAT 
GTTCAAAATG TTATTCTGAG AGAAAAGAGC CAAATACTCG AACGTATTCC GGATGAACTT 
GATAAAATTA CCGTTTTACA AGAAATCGGT AAAATTGTCG CAGCCATGAC TCATCACCCA 
GCAATTACTC TTGATGACAA AATGAAATAT GCGGCTCTGC GGGACGGATT ACGTGCTCAA 
ATAGAAACAT TGTTTAGACA ATACTACACA ACTCAGAAAC AGTTGGAGCA AAGCAATCTT 
GTTGTACCAT CATCACAAGC TGTTGGCGAT GAGAATGATA CTGATTCCGA CCATGAATCA 
GAGGAAGAAT TTGAGCCACT ATCAGGTGTT ATGGGTTCGC GTTTCGAGAG TAATCCAGTT 
CAGCCGTCGC CATCAGTTCC GGGAACATTC TTTATCATTC GATTTATTGG ACGACAAATT 
GAGAAGAACG ATAATCTTTT CAGCCTGGAG ATTCATTGGA GTGATGGTGA CAGAACATTT 
GCTCAAAGAT CTCGTGATCA ATTGAAAGCT CTACAGCATC GTCTTCTTGA TGAGTTCGGA 
CAACAGAGAA GTGAAAAATA TCTTCATCAA GGATGCACTA CATCCTACTC TTCATTTGAC 
GATGATAACA AAAAGCTTTC AGCGAGCACA TCTACAATGG AGACGTTTGC TCCAAACGGA 
GAGCGTATTG TTCCACGTCT TGCTCGTGAT GCGACTCCAG CTCAATATGT TCAATACATC 
AATGAACTTT CTGACTTGCC AGCTAGAATG ATGCTGTCAG CTGTAATATG TGAAGAGTTC 
AACGGTACCA GAGCGAAAAC AGAAGATCTA TTGCAGGAAA CTCGCGAAGC ATCGGATGGA 
TTAATATATT CTCGATGGAA GAATCCACGT GCCAAATCTC CAGTTCGTTA CTTCAAACGA 
AATGCAACAG GAAGCATTGA CCCGATTGAA TTGCCTGTCA ATATGCAACC ATTCCTTTAT 
TCAAATATCC CGCAAACACA AGTGCAGACG CTGTTCCCAA GTTGCTCTAA TTGTGGCGGT 
CCTCACGCTC CGAAACACTG TGAAAAACAA ACATTGTTAT CGAAGAAAGG TGATCATAGA 
ACTCGTTCAG ACGGGGAAGG TTCTCAGCAA AATGGTGGAA CTTCCTCTTC CAATTCATAC 
GCTCCAATTC ATGCAAACCT CCCCCATGCA GTATACATTG AAAATCCGCA AGCAATGTTA 
GGATCAAATC ACTTCAATCA TCATCAACAA CAGCACATAA TTCAGAATAC TATATTCCAT 
AATGGTGGAC AATTTCGTGC AAACGGAACA TATGAACCGC AAATTCCGAT AGCATACTAT 
CATGCCCAGG GAACTGTACA AAACTCAAAT AATACTGGTG GAGTCAACGG AAACAGTGGG 
GGCGGGAACC AGAATTCGAA TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gls-1 ( NM_001263949.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001250878.1 Caenorhabditis elegans Germline survival defective-1 (gls-1), mRNA.

Price & Availability↑

CloneID	OCa336569
Clone ID Related Accession (Same CDS sequence)	NM_001263949.1 , NM_001263949.2
Accession Version	NM_001263949.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Germline survival defective-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001263949.1.

ATGGCCAATA AAAAGAACCA TCAGCAAAAG AAAAGTACAG ACGGGTCAAC GATGAACGGG 
AATAACGCAA CAGCAACTGC CGCTGCAACA ACTCAGAATT CATCACAAAT TGGACAAAAT 
TCCAATTCCA GTCATAGCGT CACCAACGAT AACACTTCCA GCAATAACGC TTCGACGTCT 
ACATCGTCCA TCACCTCAAC TACCACTACG ACCACTACAG TACCTTCTTC ACAGCAGAGT 
CAATCTCAAA ATCAATATTC ACATCAAAGA CTTTCATCGA CTTCTTCAAC GTCACAGCAA 
ACTGGAATTT CTAAATTTCC TGCCAAAAAG GCTGGGCACA ACGAGCTTGA GAAGACAAGC 
AACACTGCTA ATTCACAATC GGGTGCTTTC AAAGGAACCA CAAATAAAGA TCGGCCGAAG 
GAAAAGGAGA AAAACACTCA CAGTGGTAAT AGGCAAAGTC AGGTTAACCA TAATCATCAT 
CATTCAAACC ATGTTAGCGG AAATCAGCAG AACCGAAAGA ATAAGAATCG TGATCATTCG 
AACCAAAGTT ATCGAGGTGG CTACACACAT AACCACAATA ATTATCACTC GTTGGAAAAT 
GCTAAATCTT CTGGATTTCT GTCAAATTCT TCCCTATCCT CTGCGGGACA AATATCAGCG 
TCTTCTGCTC CTCCAGTGAG CACAACTCCG ACTGCTATTC CCATCTTTCA AGAATCCAAA 
ATAGTTCAAA CAGATCCACC AGTCGAAGAA GCAAAAAAGA AGAAGGAAAA ACCAAAAATC 
AAGCGTGATG AAGAGCCGAT GCCATATAAG AGCACTGATC CGAAAAATAT GGATTCTGTG 
ATGGCATTCA AAGAGCACGA TGAATGGGAT AAGGTGGAGT TTATGTGTGA ACTTGTTTCT 
TTCCTGTCTC CAACCGATCT TCGCCTGCTT GGAAATTGTA TCGAAGGATC TGTTCGTTGC 
TATAACAATC AAATGCGGCC AGTAGAAAAG ACTTCAAACT GTTCGGATCC AACAGCTGGT 
TTACCTCAAT TCGTTTGCTC TCCTCCACCT CCACCGCAAC AAGTTTATTA TTTTCCTGGT 
ATTGCGGATG CTGCCCTGTA TCAAACAAGA AATGCAGTTA ACTCGGTTTT TGTACAGCAA 
CATCCACCTG GTCTTCCACC GTTATTAGGC GGAATGCAGA ATATTATGTA TCCACCTGAT 
GCTAACTTTC ATCATTCAAC CTCTTGTACG ACACCTTCAT CATCAGTTTC CGTTGCAAAT 
AAGGATGTTA ACAATGTACC AGTTGTGCTA TCAAGTAGTG TGAACGGAAT AAGCAATAAT 
ATTCCATCAG ATAGACAACA GCTAGATTCC AAACCAAATA CTGCAAGAGG TTCGAGCGGC 
AATATCAACC AATCTAATAC AACATCACCA GAACCGGAGC CCGGTTCTAA TCACATCTCC 
ACCACAGCAG CAAACCCTGC ACATCCAACT TCAGTGACTG TACCACAGTC GGTACCACCG 
GTCCCTCAAG AACCAGAATT GTTTCTCAAA TCTGTTCTCG ATCTTACCAG TTACATATAC 
ACTCTCATGG CAGTTTGTTC ATCAACAAAT AGAAAATCAG CTGCTAAGAT CTCTGACTAT 
GTTCAAAATG TTATTCTGAG AGAAAAGAGC CAAATACTCG AACGTATTCC GGATGAACTT 
GATAAAATTA CCGTTTTACA AGAAATCGGT AAAATTGTCG CAGCCATGAC TCATCACCCA 
GCAATTACTC TTGATGACAA AATGAAATAT GCGGCTCTGC GGGACGGATT ACGTGCTCAA 
ATAGAAACAT TGTTTAGACA ATACTACACA ACTCAGAAAC AGTTGGAGCA AAGCAATCTT 
GTTGTACCAT CATCACAAGC TGTTGGCGAT GAGAATGATA CTGATTCCGA CCATGAATCA 
GAGGAAGAAT TTGAGCCACT ATCAGGTGTT ATGGGTTCGC GTTTCGAGAG TAATCCAGTT 
CAGCCGTCGC CATCAGTTCC GGGAACATTC TTTATCATTC GATTTATTGG ACGACAAATT 
GAGAAGAACG ATAATCTTTT CAGCCTGGAG ATTCATTGGA GTGATGGTGA CAGAACATTT 
GCTCAAAGAT CTCGTGATCA ATTGAAAGCT CTACAGCATC GTCTTCTTGA TGAGTTCGGA 
CAACAGAGAA GTGAAAAATA TCTTCATCAA GGATGCACTA CATCCTACTC TTCATTTGAC 
GATGATAACA AAAAGCTTTC AGCGAGCACA TCTACAATGG AGACGTTTGC TCCAAACGGA 
GAGCGTATTG TTCCACGTCT TGCTCGTGAT GCGACTCCAG CTCAATATGT TCAATACATC 
AATGAACTTT CTGACTTGCC AGCTAGAATG ATGCTGTCAG CTGTAATATG TGAAGAGTTC 
AACGGTACCA GAGCGAAAAC AGAAGATCTA TTGCAGGAAA CTCGCGAAGC ATCGGATGGA 
TTAATATATT CTCGATGGAA GAATCCACGT GCCAAATCTC CAGTTCGTTA CTTCAAACGA 
AATGCAACAG GAAGCATTGA CCCGATTGAA TTGCCTGTCA ATATGCAACC ATTCCTTTAT 
TCAAATATCC CGCAAACACA AGTGCAGACG CTGTTCCCAA GTTGCTCTAA TTGTGGCGGT 
CCTCACGCTC CGAAACACTG TGAAAAACAA ACATTGTTAT CGAAGAAAGG TGATCATAGA 
ACTCGTTCAG ACGGGGAAGG TTCTCAGCAA AATGGTGGAA CTTCCTCTTC CAATTCATAC 
GCTCCAATTC ATGCAAACCT CCCCCATGCA GTATACATTG AAAATCCGCA AGCAATGTTA 
GGATCAAATC ACTTCAATCA TCATCAACAA CAGCACATAA TTCAGAATAC TATATTCCAT 
AATGGTGGAC AATTTCGTGC AAACGGAACA TATGAACCGC AAATTCCGAT AGCATACTAT 
CATGCCCAGG GAACTGTACA AAACTCAAAT AATACTGGTG GAGTCAACGG AAACAGTGGG 
GGCGGGAACC AGAATTCGAA TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001250878.1
CDS	69..3095
Translation	MANKKNHQQKKSTDGSTMNGNNATATAAATTQNSSQIGQNSNSSHSVTNDNTSSNNASTSTSSITSTTTTTTTVPSSQQSQSQNQYSHQRLSSTSSTSQQTGISKFPAKKAGHNELEKTSNTANSQSGAFKGTTNKDRPKEKEKNTHSGNRQSQVNHNHHHSNHVSGNQQNRKNKNRDHSNQSYRGGYTHNHNNYHSLENAKSSGFLSNSSLSSAGQISASSAPPVSTTPTAIPIFQESKIVQTDPPVEEAKKKKEKPKIKRDEEPMPYKSTDPKNMDSVMAFKEHDEWDKVEFMCELVSFLSPTDLRLLGNCIEGSVRCYNNQMRPVEKTSNCSDPTAGLPQFVCSPPPPPQQVYYFPGIADAALYQTRNAVNSVFVQQHPPGLPPLLGGMQNIMYPPDANFHHSTSCTTPSSSVSVANKDVNNVPVVLSSSVNGISNNIPSDRQQLDSKPNTARGSSGNINQSNTTSPEPEPGSNHISTTAANPAHPTSVTVPQSVPPVPQEPELFLKSVLDLTSYIYTLMAVCSSTNRKSAAKISDYVQNVILREKSQILERIPDELDKITVLQEIGKIVAAMTHHPAITLDDKMKYAALRDGLRAQIETLFRQYYTTQKQLEQSNLVVPSSQAVGDENDTDSDHESEEEFEPLSGVMGSRFESNPVQPSPSVPGTFFIIRFIGRQIEKNDNLFSLEIHWSDGDRTFAQRSRDQLKALQHRLLDEFGQQRSEKYLHQGCTTSYSSFDDDNKKLSASTSTMETFAPNGERIVPRLARDATPAQYVQYINELSDLPARMMLSAVICEEFNGTRAKTEDLLQETREASDGLIYSRWKNPRAKSPVRYFKRNATGSIDPIELPVNMQPFLYSNIPQTQVQTLFPSCSNCGGPHAPKHCEKQTLLSKKGDHRTRSDGEGSQQNGGTSSSNSYAPIHANLPHAVYIENPQAMLGSNHFNHHQQQHIIQNTIFHNGGQFRANGTYEPQIPIAYYHAQGTVQNSNNTGGVNGNSGGGNQNSNF

Target ORF information:

RefSeq Version	NM_001263949.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Germline survival defective-1 (gls-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001263949.2

ORF Insert Sequence:

ATGGCCAATA AAAAGAACCA TCAGCAAAAG AAAAGTACAG ACGGGTCAAC GATGAACGGG 
AATAACGCAA CAGCAACTGC CGCTGCAACA ACTCAGAATT CATCACAAAT TGGACAAAAT 
TCCAATTCCA GTCATAGCGT CACCAACGAT AACACTTCCA GCAATAACGC TTCGACGTCT 
ACATCGTCCA TCACCTCAAC TACCACTACG ACCACTACAG TACCTTCTTC ACAGCAGAGT 
CAATCTCAAA ATCAATATTC ACATCAAAGA CTTTCATCGA CTTCTTCAAC GTCACAGCAA 
ACTGGAATTT CTAAATTTCC TGCCAAAAAG GCTGGGCACA ACGAGCTTGA GAAGACAAGC 
AACACTGCTA ATTCACAATC GGGTGCTTTC AAAGGAACCA CAAATAAAGA TCGGCCGAAG 
GAAAAGGAGA AAAACACTCA CAGTGGTAAT AGGCAAAGTC AGGTTAACCA TAATCATCAT 
CATTCAAACC ATGTTAGCGG AAATCAGCAG AACCGAAAGA ATAAGAATCG TGATCATTCG 
AACCAAAGTT ATCGAGGTGG CTACACACAT AACCACAATA ATTATCACTC GTTGGAAAAT 
GCTAAATCTT CTGGATTTCT GTCAAATTCT TCCCTATCCT CTGCGGGACA AATATCAGCG 
TCTTCTGCTC CTCCAGTGAG CACAACTCCG ACTGCTATTC CCATCTTTCA AGAATCCAAA 
ATAGTTCAAA CAGATCCACC AGTCGAAGAA GCAAAAAAGA AGAAGGAAAA ACCAAAAATC 
AAGCGTGATG AAGAGCCGAT GCCATATAAG AGCACTGATC CGAAAAATAT GGATTCTGTG 
ATGGCATTCA AAGAGCACGA TGAATGGGAT AAGGTGGAGT TTATGTGTGA ACTTGTTTCT 
TTCCTGTCTC CAACCGATCT TCGCCTGCTT GGAAATTGTA TCGAAGGATC TGTTCGTTGC 
TATAACAATC AAATGCGGCC AGTAGAAAAG ACTTCAAACT GTTCGGATCC AACAGCTGGT 
TTACCTCAAT TCGTTTGCTC TCCTCCACCT CCACCGCAAC AAGTTTATTA TTTTCCTGGT 
ATTGCGGATG CTGCCCTGTA TCAAACAAGA AATGCAGTTA ACTCGGTTTT TGTACAGCAA 
CATCCACCTG GTCTTCCACC GTTATTAGGC GGAATGCAGA ATATTATGTA TCCACCTGAT 
GCTAACTTTC ATCATTCAAC CTCTTGTACG ACACCTTCAT CATCAGTTTC CGTTGCAAAT 
AAGGATGTTA ACAATGTACC AGTTGTGCTA TCAAGTAGTG TGAACGGAAT AAGCAATAAT 
ATTCCATCAG ATAGACAACA GCTAGATTCC AAACCAAATA CTGCAAGAGG TTCGAGCGGC 
AATATCAACC AATCTAATAC AACATCACCA GAACCGGAGC CCGGTTCTAA TCACATCTCC 
ACCACAGCAG CAAACCCTGC ACATCCAACT TCAGTGACTG TACCACAGTC GGTACCACCG 
GTCCCTCAAG AACCAGAATT GTTTCTCAAA TCTGTTCTCG ATCTTACCAG TTACATATAC 
ACTCTCATGG CAGTTTGTTC ATCAACAAAT AGAAAATCAG CTGCTAAGAT CTCTGACTAT 
GTTCAAAATG TTATTCTGAG AGAAAAGAGC CAAATACTCG AACGTATTCC GGATGAACTT 
GATAAAATTA CCGTTTTACA AGAAATCGGT AAAATTGTCG CAGCCATGAC TCATCACCCA 
GCAATTACTC TTGATGACAA AATGAAATAT GCGGCTCTGC GGGACGGATT ACGTGCTCAA 
ATAGAAACAT TGTTTAGACA ATACTACACA ACTCAGAAAC AGTTGGAGCA AAGCAATCTT 
GTTGTACCAT CATCACAAGC TGTTGGCGAT GAGAATGATA CTGATTCCGA CCATGAATCA 
GAGGAAGAAT TTGAGCCACT ATCAGGTGTT ATGGGTTCGC GTTTCGAGAG TAATCCAGTT 
CAGCCGTCGC CATCAGTTCC GGGAACATTC TTTATCATTC GATTTATTGG ACGACAAATT 
GAGAAGAACG ATAATCTTTT CAGCCTGGAG ATTCATTGGA GTGATGGTGA CAGAACATTT 
GCTCAAAGAT CTCGTGATCA ATTGAAAGCT CTACAGCATC GTCTTCTTGA TGAGTTCGGA 
CAACAGAGAA GTGAAAAATA TCTTCATCAA GGATGCACTA CATCCTACTC TTCATTTGAC 
GATGATAACA AAAAGCTTTC AGCGAGCACA TCTACAATGG AGACGTTTGC TCCAAACGGA 
GAGCGTATTG TTCCACGTCT TGCTCGTGAT GCGACTCCAG CTCAATATGT TCAATACATC 
AATGAACTTT CTGACTTGCC AGCTAGAATG ATGCTGTCAG CTGTAATATG TGAAGAGTTC 
AACGGTACCA GAGCGAAAAC AGAAGATCTA TTGCAGGAAA CTCGCGAAGC ATCGGATGGA 
TTAATATATT CTCGATGGAA GAATCCACGT GCCAAATCTC CAGTTCGTTA CTTCAAACGA 
AATGCAACAG GAAGCATTGA CCCGATTGAA TTGCCTGTCA ATATGCAACC ATTCCTTTAT 
TCAAATATCC CGCAAACACA AGTGCAGACG CTGTTCCCAA GTTGCTCTAA TTGTGGCGGT 
CCTCACGCTC CGAAACACTG TGAAAAACAA ACATTGTTAT CGAAGAAAGG TGATCATAGA 
ACTCGTTCAG ACGGGGAAGG TTCTCAGCAA AATGGTGGAA CTTCCTCTTC CAATTCATAC 
GCTCCAATTC ATGCAAACCT CCCCCATGCA GTATACATTG AAAATCCGCA AGCAATGTTA 
GGATCAAATC ACTTCAATCA TCATCAACAA CAGCACATAA TTCAGAATAC TATATTCCAT 
AATGGTGGAC AATTTCGTGC AAACGGAACA TATGAACCGC AAATTCCGAT AGCATACTAT 
CATGCCCAGG GAACTGTACA AAACTCAAAT AATACTGGTG GAGTCAACGG AAACAGTGGG 
GGCGGGAACC AGAATTCGAA TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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gls-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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