rme-8 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	rme-8
Entrez Gene ID	172594
Full Name	Receptor Mediated Endocytosis
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001026224.3	NP_001021395.1	Receptor Mediated Endocytosis
NM_001026224.4	NP_001021395.1	J domain-containing protein
NM_059821.5	NP_492222.2	J domain-containing protein
NM_059821.4	NP_492222.2	Receptor Mediated Endocytosis

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Dnajc13	XP_003750628.1
Homo sapiens (human)	DNAJC13	NP_056083.3
Pan troglodytes (chimpanzee)	DNAJC13	XP_516755.3
	DNAJC13	XP_001115526.1
Mus musculus (house mouse)	Dnajc13	NP_001156498.1
Drosophila melanogaster (fruit fly)	Rme-8	NP_610467.1
Arabidopsis thaliana (thale cress)	GRV2	NP_180257.3
Oryza sativa (rice)	Os10g0575200	NP_001065479.1
Canis lupus familiaris (dog)	DNAJC13	XP_542783.3
Bos taurus (cattle)	DNAJC13	NP_001107217.1
Gallus gallus (chicken)	DNAJC13	XP_004939502.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005771	XP_315786.4
Caenorhabditis elegans (roundworm)	rme-8	NP_492222.2
Xenopus tropicalis (tropical clawed frog)	dnajc13	XP_002937780.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of SNX-1, RME-8, or the clathrin chaperone Hsc70/HSP-1 leads to over-accumulation of endosomal clathrin, reduced clathrin dynamics, and missorting of MIG-14 to the lysosome.
Title: Regulation of endosomal clathrin and retromer-mediated endosome to Golgi retrograde transport by the J-domain protein RME-8.

The following rme-8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rme-8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335309	NM_059821.5 Latest version!	Caenorhabditis elegans J domain-containing protein (rme-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa335310	NM_001026224.4 Latest version!	Caenorhabditis elegans J domain-containing protein (rme-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCa335310	NM_001026224.3	Caenorhabditis elegans Receptor Mediated Endocytosis (rme-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa335309	NM_059821.4	Caenorhabditis elegans Receptor Mediated Endocytosis (rme-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rme-8 ( NM_059821.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492222.2 Caenorhabditis elegans J domain-containing protein (rme-8), mRNA.

Price & Availability↑

CloneID	OCa335309
Clone ID Related Accession (Same CDS sequence)	NM_059821.5 , NM_059821.4
Accession Version	NM_059821.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	J domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059821.4.

ATGGCTCAAT TTTGCTCGGA AAACCGAGAC ATTGCATGCT ATTTGGTCAC AAAACACTCA 
TGGAAGGGAA AGTACAAACG GGTGTTTTCT ATCGGGACAC TGGCCATCAC CACTTACAAT 
CCTAATACCT TGGAGATTAC CAATCAATGG CTCTACGAGG ACTTCTTATC AGTGAAACCA 
CTTCGAAATG GAGGTTCAAA TGATTCAAAA CAGGATGAAT ACAAGATTCA TGTTAGGAAT 
CGTGGAAAAA ACAATGATAT GCGTTTTTCA TCTGATTATA CCACCGATAT ACTTACACAT 
TGTCTGCAAT TCAGCACCAA GTTCGCTGAT AAGAACTTTG AACCTTTGAC GGTAACTGCA 
TTCAAACAAA GTTGGAGTGA TCGTCGAATT CCCGTAACTC TACGAGCAAA TGCTTCTTGT 
TTGGAGCAAA TTGATAATCG TGGAGTCGTC GTTCAAAGTT ATCCGTACAA AAATATTCGA 
AGTATTGGAA AAGTGTCCGA TTGTCCCGGT GGTTTTGTGG TTGATGTGGG AGAACATAGA 
CGGAGGCACA TGTTCGCTTC TTCAAACAAC GAAGAACTAG TGAAAGAGAT TCGTCGTTTA 
GCATCTGATA ATATCGGAAT TATCGTTCCA ATTGCTAAAG AGCAGCTAAC ACTCGAAGAT 
TTTATGAGAA CTCGACTCGG ATTGTGCAGT CGAGATGAAG AGCTCACTTC ATATGCTGAA 
TTCAAAATTT CCAAAATTAC TCGAAGAAAT GAGATGCCTG TTCGACGGCT TCTATGTCTC 
AGTGAGACGT GTATTATTGA AAGAGACTTG GCTACTTATG CAGTGATTTG TGCAACTCCA 
CTGAAACATA TCGTTTGCCT TGTTCGAAGT GAAAAAGATC CCCAACAATT CATTGTTGAA 
TACGAAAATG GAGATGGGCG AGCATATGTT GCTGCTGAAA GAGATTTGAT CTTAGCCTCA 
CTCTTAGATG GAATTCGTGC TTCTGGAAAT AATGAAGTGT TCGTTTGTGG ACACAGATTT 
GAAAGGAATT TGAGAGTGAT CCCATTCTCA ACGAATTTGG ATGAAGATAG TGAATCTCAA 
TGTATGAAGC ATATTATTGC ACCTCCACCT GGACTTCGTC GTTGTGATTT GATCCGTCGC 
TTCAACGCAA ATGTTCCATA TTCTGGTCTT CGATTTTCAA AGTCGCACGA AGGATTCTTC 
TCAGAAAACA AAGGAAAAGT TATTGTAAAT GCAATTGAGG CAGTTCTAAT GGAGAATTAT 
ACAAAAGACG ATAAGGAGTA CAAGCACAAA ACTGAAGCTC AGTTACAGTG TTTACGAAGA 
CTTTTTGCGT CGAAAAGTGG ATTCCAGGCA TTTACAGAAG TTAATGGTGT TCGTGAGAAG 
CTTGGGAGTC TTGTGGTTCG AGTTCTATCT TGGAAATCTG AATCAATTGA TCATTCGACG 
GTTGAAGCTC TTTGTGCATT GATGTATCCA ATGCATGATC AATATGAACT TAGAATAGAG 
CAACTGAACA AGCAGAGTCT GATGAGCAGC CCAAAATTTG TGGAGAACTT ACTCGATTTG 
ATTGTTCTTC ACGTGGATCG TTCAACTGGA TGGCTTGTGA TTGCATCAAT GCTCAATTTC 
CTCACATTTT CCGTTTGCTC TCCGTACTCT GAAACCACCG CTGGAGATAC TTTCGATCAT 
ATTCTGAAAC TCGTATCTCT CCGAGGGCGC AGCTTTTTCC GTTTATTCCA ATGTCCCAGT 
ATGACAATTG TTAAAGGTGC TGGAATGGTT ATGAGAGCAA TTATCGAAGA AGCTGATGTT 
GAAACTTCAA AATCAATGCA AATGTTAGCA CTATCAGAAG GAGCATTCTT GACACATTTG 
TTCATGTCAT TATTGTCAAC TGGAAAAGAT TTACGAGTAA TGACAAATAA ACAATTGTCT 
GGACATTTGA TAAGTCTTTG GATAGCTGAT AATCAACAGG CTAATGATTT GTTAATTAGG 
TGCCTGCCCC GTGGTCTATT GAACTATATG GAGTCAGACG AAAAAGTTCC AGTAAACGAG 
AAAGATCTTC TCATTGTTCG AAATAACTTT GATGCAGCAT CAAATGAAAC TAAGCAGAAT 
GCGATGAAGG AGAAGTTTGA CCAGCTTCGA GTTACTGCTG AAGCTGGTTT GGAAAGATTT 
GTGCAGCACT GGGATCTCGA ACAAAAACTC AATTTTCTTC CAAAACGAGT TATTGATGAG 
AAACAACAAC AAAGACAGCA ACCGGTTGTC CTGAGAAAAC GAAGAAATCG AGTACGGCCA 
AATGTTAATT GGAAACTATT TGCATATCAA TTCTCGAAGG ATCACTCTCA GGCAGATTTA 
ATTTGGAATG AGAAGACGCG AGAAGAGTTC CGTCATGCAA TGGATACAGA AACACGAGCT 
CTTATCAGTG AAAAAGAACA AGCACCAACT GGATTGCCGA TTGCTTGGAA TCATACTGAG 
TTCCAAATCA GATATCCATC ACTTTTAGAA GAAATTAAAA TTGGAGATTA TTACCTTCGA 
TTGCTTCTGA TTGAGGCAGA CGAAAATGCG ACTCCGATTC ATAATCCGCT GGAGTTCTTC 
AACAACGTCT ATCATCGTTT CCTACTTTCC ACTAAAGTTG ACATGAAATG TCTGTGCCTT 
CGTGCAATGG CTATCACCTA TTCTCGACAT CATATGACAA TCGGAGCATT TCAGGATTCA 
AAGTATTTCG TAGAAATGCT TCAAAAATGC ATAAATCCGT TGGAAAGAGA TCATCTTGTG 
CTTCTGTTAT CGAAACTAGC ACTGAATAAG GATAATGTTC GAGAACTTAT AATATCCAAT 
ATTCTTCCAC TTTTGGTAGA TCTTTGTGTT CTTGCACATT TACACGTGCA GCGAGCAAAA 
GTTCAAAATC AGACAAATGT GATTGAGGCA AGTGCCGAAC AAATGGCTGA AGGAGGTTCT 
GAAGAATGGT ATTATCATGA TAAGGATGCT AAACAAGTCG GACCATTGTC ATTTGAGAAA 
ATGAAGTCTC TTTACACGGA GAAGACTATT TTTGAGAAAT CTCAAATTTG GGCAGCTGGA 
ATGGACAAGT GGATGAGTTT GGCTGCAGTT CCACAATTCA GATGGACTGT TTGTCAGCAA 
AAAGATCAGC CGAACGAGAT TAATACAGGA AAAGGACCAT TCCAGGCAAC TGTCATGAAC 
TTCACAGATC TCAGTGTTCT TTGTTTAGAT ACTATTCTTC AGATGTGCGA ATTTTTCCCA 
TCAAGGGATT CTCATGATTG TGTGGTCCGT CCGATGCCAT CTGTCAAGAA GCAGCTCACT 
GAGCCAGTAC TCCTTTATCA ACTCGTTCAA CTTCTTCTAA CCTATGAACC ACAGATTGTG 
CAGAGAGTTG CTCTACTACT GTATCTTGTA ATGCAGGATA ACCCATTTCT TCCACGACTT 
TATCTTTCTG GTGTCTTCTA TTTTATTCTT ATGTACAATG GATCAAATGT TCTTCCGATT 
GCTCGATTCC TTCACTATAC CCACATGAAA CAAGCATTCC GTTCTACACT TCCACAATTT 
GAGGGACAAC GGCAATCAGT TCTTGCCACT CTTCTTCCAG AAGCCGCCAC ATTCTATTTG 
GAACAATATG GACCTGAAAA GTATGCCGAA GTGTTCCTTG GAGAATTCGA TAATCCCGAA 
ATCATTTGGA ATACGGCGAT GCGTCGACAT TTGATTGAAC GAATCGCTGT TCATGTTGCA 
GATTTCTCGC ATCGATTAAC ATCAAATGTT CGAGCTTTAT ATCAATTCTG TCCAATTCCT 
TTGATCGATT ATCCAGAACT TGCTCAGGAA CTCTTCTGTC ATGTTTATTA TCTTCGACAC 
TTGTGTAATA AGCAAAGATT CCCTGATTGG GAAATTAGGG ATCCGATTCC GTTCCTTCGA 
TGTTGCCTTG CCACATGGTA TAATGAGCTA GAGAAGAAGC CGGCGACGAT GTCAGTCGAT 
CTTGCAAGAG AGATTTTATC AGTTGATTTG ACTAACGAAG AACACCGAAA ACCTGCATTC 
ATACGTCGTC AATATTACAA ACTCGCTGCT AAATACCATC CTGACAAGAA TCCAGAAGGA 
AGAGAAATGT TCGAACGAAT AAATGCAGCT TATGAACTAT TGAGCAGTGA AACTGCAAAT 
AATTCAGGAA TGCCGGATTC GCATCGAATT GTGCTTTGTT TACAGGCACA ATCCATCATT 
TATAGTAGAT ATAGTCAAGA ATTATCCGAA TACAAATACG CTGGTTACTC TCAACTCATT 
AAAACTATCG ATTTGGAAGC AAAAGACGAA GCATTGTTCA TAAAAGGAGG TGGAGACCTG 
TTGTCAGCTG CTATTGAACT TGCTAATTAT ACACTTATTA GTAGTGCTTT GAATGCTGAA 
CAATTGAGAA GAGATAATGG ATTAGAGGCA TTAGTAACTG CATTTGATCG ATGTGTTCCA 
ATGGTAACAA TGAGTTCATT ACCTGATGAT ATGGCTGTTC GAGTTTGTAT TCATGTTTGT 
GATTGTTTTG CCACTGCTGC AACATTTGAA GCGTGTAGGC AGCGACTGAT GGAGATGCCA 
TCCATATTTG GAGCCCTCAC TAGATTACTT CAATTTTCGA ACTTACCCCG TCTCTCCACT 
GCAGCAGCTC AATGTATTCG TGCAATGGCA GTTGACACAC TTCTTCAATT CCAACTTTTC 
CAAACTGGTG TTCTATGGCA ATTGGTACCA CATCTCTTTC ATTTCGATTA TACATTGGAT 
GAAGGAGGTG TTCAACATTC AGAGGATTCT AATAAACAAT CACTCGCTAA TTCATTAGCT 
CGTTCTTCGT GTGAAGCTCT TGCAGCTCTG GCTGGATTCC GTGAGAATAC ACCGGATAAT 
GATGGAGTTC AGGCAAGCCT TCGAGCTCTA CTTACTCCTT ACATCTGTCG ATGCATGAAG 
CTTGAAACAA ATGATATGGT TCTGAAAACG CTGAATAGTA ATATGGAAAA TCCATATATG 
ATCTGGGATA ATGGAACAAG AGCTGAAGTT CTCGAATTTG TGGAGAGACA TCGAACTTCT 
AATGAGCCTA CGAGTGAGCT GTACGGAGCC GAATTCGAGT TATCTGTGCA CAAAAATGAG 
CTTATCGTTG GAGACATATT CATCAGAATC TACAATGAAC AGCCAACATT TGCTCTTCAC 
GAACCCAAAA AAGTTGCAAT CGATCTTTTG GATTACATCA AAAAACACTC GGCAGAACTA 
ACCGGAGCTC CAAAACCTAA AGCAATTTCG GATGATTTGA TTGAAATTGA CTGGGGAAAT 
GGATCACAGG CTAATGGAAA CTCATTGGAC ACAGAAACAA AAGTTCTCAT GACAATGACT 
GCATTAGCTA ATTTAGTTTC AGCGAATCCA GGTGCACTAT TTGCAAACAA AAAGGGTGTT 
GAAATTCTAC TAATTGGAAA CTTCCCACTT CTTATCACTT ATCTACGTTG TCGAAAACAT 
CCGAAGCTTC AAATTGCTGC ACTTCAAGTC ATTCTGTTGG CTGCAGCTAA TAAGGAATGT 
GTAACTGATT TGGCCACGTG TAATGTCACT ACAACACTTT TCACACTTTT ACGTGATCAG 
CCGAAGATGA TAGCAAGAGT ATTAGATGTT CTGATTGCAC TTTCATCAAA TGGACAAATT 
GGAAAAGAAA TGTTAGAACA TGGAGGACTT ATGTATATTC TATCAATTCT TTGTTTGACA 
AATTCTGATC AAGGCCAAAG ACTGCAGGCA GCCGAGCTTT TGGCCAAACT TCAAGCTGAT 
AAGCTTACTG GGCCAAGATG GACACGATTT ATTATCAAGT TTTTGCCAGA AATATTTGCA 
GATTCATTGA GAGATTCGCC CAATACAGCC CTTCAAATGT TCGATTCATC AAGCGAAAAT 
CCAGAGCTTA TTTGGAATGA AGTAACGCGT CAAAAAGTGA AAGGAATCAT TGATACAGAA 
GTCGGAAAAC TCTATGAACA ACACATCAAA GATCCAACTG TCAAATGGAA TACGACTTCA 
TTGGCTGACA AGAGTTGTGC CTATGGTGAT AGTATTAATG GTGAACTTGT TGTTGGAGGT 
GTTTTTGTAC GATTATACGT CGAAAATCCA GGATTCAATG TCAGACATCC AAAATTATTC 
TCAACAGAGC TCATCGAAAA AGTGCTTGAA CTGATGAGTC GTCCAACTCC AGAACTCGAT 
TTGGTCACAA AAGCCTTTGT TGAACTGGTC CGTCATCATC CGAATACTGC TGATCAATTG 
CCATCTCAAG GATATCTACC ACAGTTCTGC ACTGCAATGT GTCTTCAAAA CACATCTGCG 
TCTCGTTCAG CAATTCTAAT TCTTCAAGAA TTGAGCGAAA ATCAATTCTG TTGTGATGCT 
CTTTCTCAGC TGCCATGTAT TGATGGAATA ATGAAATCAA TGAAGAATCA ACCAAGTTTG 
ATGAGAGAAT CGGCGCACGC TTTAAAATGT CTCATGAAGA GAAATACAGG AGAATTGGCT 
CAACAAATGC TCTCATGTGG AATGGTTCCA TATTTGTTAC AAGTTTTGGA TAGTTCAATG 
AATGGAGTAT CAAACGGAGC AGCTGCTCGT GCCGAGATTG TTGACGCATT AAAAAGCGCA 
ATTTTAGATC TGAAAGTTGG TCAGAAGATT GCTGAGATTT TAGACAAGAG TCCCGTCTGG 
GCACAGTTTA AAGATCAACG ACATGATTTA TTCTTGCCAG AAGCGAGAAC TCAAGCTATT 
ACAGGAGGTC CAACTGGTGT TGCTGGATAT CTAACAGAAG GAATGTTCAA TCCACCACCA 
ATGAGCAATC AGCCACCACC AATGCATCAA ACATCTGGAG ATTTCCAACC ACCACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492222.2
CDS	48..6887
Translation	MAQFCSENRDIACYLVTKHSWKGKYKRVFSIGTLAITTYNPNTLEITNQWLYEDFLSVKPLRNGGSNDSKQDEYKIHVRNRGKNNDMRFSSDYTTDILTHCLQFSTKFADKNFEPLTVTAFKQSWSDRRIPVTLRANASCLEQIDNRGVVVQSYPYKNIRSIGKVSDCPGGFVVDVGEHRRRHMFASSNNEELVKEIRRLASDNIGIIVPIAKEQLTLEDFMRTRLGLCSRDEELTSYAEFKISKITRRNEMPVRRLLCLSETCIIERDLATYAVICATPLKHIVCLVRSEKDPQQFIVEYENGDGRAYVAAERDLILASLLDGIRASGNNEVFVCGHRFERNLRVIPFSTNLDEDSESQCMKHIIAPPPGLRRCDLIRRFNANVPYSGLRFSKSHEGFFSENKGKVIVNAIEAVLMENYTKDDKEYKHKTEAQLQCLRRLFASKSGFQAFTEVNGVREKLGSLVVRVLSWKSESIDHSTVEALCALMYPMHDQYELRIEQLNKQSLMSSPKFVENLLDLIVLHVDRSTGWLVIASMLNFLTFSVCSPYSETTAGDTFDHILKLVSLRGRSFFRLFQCPSMTIVKGAGMVMRAIIEEADVETSKSMQMLALSEGAFLTHLFMSLLSTGKDLRVMTNKQLSGHLISLWIADNQQANDLLIRCLPRGLLNYMESDEKVPVNEKDLLIVRNNFDAASNETKQNAMKEKFDQLRVTAEAGLERFVQHWDLEQKLNFLPKRVIDEKQQQRQQPVVLRKRRNRVRPNVNWKLFAYQFSKDHSQADLIWNEKTREEFRHAMDTETRALISEKEQAPTGLPIAWNHTEFQIRYPSLLEEIKIGDYYLRLLLIEADENATPIHNPLEFFNNVYHRFLLSTKVDMKCLCLRAMAITYSRHHMTIGAFQDSKYFVEMLQKCINPLERDHLVLLLSKLALNKDNVRELIISNILPLLVDLCVLAHLHVQRAKVQNQTNVIEASAEQMAEGGSEEWYYHDKDAKQVGPLSFEKMKSLYTEKTIFEKSQIWAAGMDKWMSLAAVPQFRWTVCQQKDQPNEINTGKGPFQATVMNFTDLSVLCLDTILQMCEFFPSRDSHDCVVRPMPSVKKQLTEPVLLYQLVQLLLTYEPQIVQRVALLLYLVMQDNPFLPRLYLSGVFYFILMYNGSNVLPIARFLHYTHMKQAFRSTLPQFEGQRQSVLATLLPEAATFYLEQYGPEKYAEVFLGEFDNPEIIWNTAMRRHLIERIAVHVADFSHRLTSNVRALYQFCPIPLIDYPELAQELFCHVYYLRHLCNKQRFPDWEIRDPIPFLRCCLATWYNELEKKPATMSVDLAREILSVDLTNEEHRKPAFIRRQYYKLAAKYHPDKNPEGREMFERINAAYELLSSETANNSGMPDSHRIVLCLQAQSIIYSRYSQELSEYKYAGYSQLIKTIDLEAKDEALFIKGGGDLLSAAIELANYTLISSALNAEQLRRDNGLEALVTAFDRCVPMVTMSSLPDDMAVRVCIHVCDCFATAATFEACRQRLMEMPSIFGALTRLLQFSNLPRLSTAAAQCIRAMAVDTLLQFQLFQTGVLWQLVPHLFHFDYTLDEGGVQHSEDSNKQSLANSLARSSCEALAALAGFRENTPDNDGVQASLRALLTPYICRCMKLETNDMVLKTLNSNMENPYMIWDNGTRAEVLEFVERHRTSNEPTSELYGAEFELSVHKNELIVGDIFIRIYNEQPTFALHEPKKVAIDLLDYIKKHSAELTGAPKPKAISDDLIEIDWGNGSQANGNSLDTETKVLMTMTALANLVSANPGALFANKKGVEILLIGNFPLLITYLRCRKHPKLQIAALQVILLAAANKECVTDLATCNVTTTLFTLLRDQPKMIARVLDVLIALSSNGQIGKEMLEHGGLMYILSILCLTNSDQGQRLQAAELLAKLQADKLTGPRWTRFIIKFLPEIFADSLRDSPNTALQMFDSSSENPELIWNEVTRQKVKGIIDTEVGKLYEQHIKDPTVKWNTTSLADKSCAYGDSINGELVVGGVFVRLYVENPGFNVRHPKLFSTELIEKVLELMSRPTPELDLVTKAFVELVRHHPNTADQLPSQGYLPQFCTAMCLQNTSASRSAILILQELSENQFCCDALSQLPCIDGIMKSMKNQPSLMRESAHALKCLMKRNTGELAQQMLSCGMVPYLLQVLDSSMNGVSNGAAARAEIVDALKSAILDLKVGQKIAEILDKSPVWAQFKDQRHDLFLPEARTQAITGGPTGVAGYLTEGMFNPPPMSNQPPPMHQTSGDFQPPL

Target ORF information:

RefSeq Version	NM_059821.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans J domain-containing protein (rme-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059821.5

ORF Insert Sequence:

ATGGCTCAAT TTTGCTCGGA AAACCGAGAC ATTGCATGCT ATTTGGTCAC AAAACACTCA 
TGGAAGGGAA AGTACAAACG GGTGTTTTCT ATCGGGACAC TGGCCATCAC CACTTACAAT 
CCTAATACCT TGGAGATTAC CAATCAATGG CTCTACGAGG ACTTCTTATC AGTGAAACCA 
CTTCGAAATG GAGGTTCAAA TGATTCAAAA CAGGATGAAT ACAAGATTCA TGTTAGGAAT 
CGTGGAAAAA ACAATGATAT GCGTTTTTCA TCTGATTATA CCACCGATAT ACTTACACAT 
TGTCTGCAAT TCAGCACCAA GTTCGCTGAT AAGAACTTTG AACCTTTGAC GGTAACTGCA 
TTCAAACAAA GTTGGAGTGA TCGTCGAATT CCCGTAACTC TACGAGCAAA TGCTTCTTGT 
TTGGAGCAAA TTGATAATCG TGGAGTCGTC GTTCAAAGTT ATCCGTACAA AAATATTCGA 
AGTATTGGAA AAGTGTCCGA TTGTCCCGGT GGTTTTGTGG TTGATGTGGG AGAACATAGA 
CGGAGGCACA TGTTCGCTTC TTCAAACAAC GAAGAACTAG TGAAAGAGAT TCGTCGTTTA 
GCATCTGATA ATATCGGAAT TATCGTTCCA ATTGCTAAAG AGCAGCTAAC ACTCGAAGAT 
TTTATGAGAA CTCGACTCGG ATTGTGCAGT CGAGATGAAG AGCTCACTTC ATATGCTGAA 
TTCAAAATTT CCAAAATTAC TCGAAGAAAT GAGATGCCTG TTCGACGGCT TCTATGTCTC 
AGTGAGACGT GTATTATTGA AAGAGACTTG GCTACTTATG CAGTGATTTG TGCAACTCCA 
CTGAAACATA TCGTTTGCCT TGTTCGAAGT GAAAAAGATC CCCAACAATT CATTGTTGAA 
TACGAAAATG GAGATGGGCG AGCATATGTT GCTGCTGAAA GAGATTTGAT CTTAGCCTCA 
CTCTTAGATG GAATTCGTGC TTCTGGAAAT AATGAAGTGT TCGTTTGTGG ACACAGATTT 
GAAAGGAATT TGAGAGTGAT CCCATTCTCA ACGAATTTGG ATGAAGATAG TGAATCTCAA 
TGTATGAAGC ATATTATTGC ACCTCCACCT GGACTTCGTC GTTGTGATTT GATCCGTCGC 
TTCAACGCAA ATGTTCCATA TTCTGGTCTT CGATTTTCAA AGTCGCACGA AGGATTCTTC 
TCAGAAAACA AAGGAAAAGT TATTGTAAAT GCAATTGAGG CAGTTCTAAT GGAGAATTAT 
ACAAAAGACG ATAAGGAGTA CAAGCACAAA ACTGAAGCTC AGTTACAGTG TTTACGAAGA 
CTTTTTGCGT CGAAAAGTGG ATTCCAGGCA TTTACAGAAG TTAATGGTGT TCGTGAGAAG 
CTTGGGAGTC TTGTGGTTCG AGTTCTATCT TGGAAATCTG AATCAATTGA TCATTCGACG 
GTTGAAGCTC TTTGTGCATT GATGTATCCA ATGCATGATC AATATGAACT TAGAATAGAG 
CAACTGAACA AGCAGAGTCT GATGAGCAGC CCAAAATTTG TGGAGAACTT ACTCGATTTG 
ATTGTTCTTC ACGTGGATCG TTCAACTGGA TGGCTTGTGA TTGCATCAAT GCTCAATTTC 
CTCACATTTT CCGTTTGCTC TCCGTACTCT GAAACCACCG CTGGAGATAC TTTCGATCAT 
ATTCTGAAAC TCGTATCTCT CCGAGGGCGC AGCTTTTTCC GTTTATTCCA ATGTCCCAGT 
ATGACAATTG TTAAAGGTGC TGGAATGGTT ATGAGAGCAA TTATCGAAGA AGCTGATGTT 
GAAACTTCAA AATCAATGCA AATGTTAGCA CTATCAGAAG GAGCATTCTT GACACATTTG 
TTCATGTCAT TATTGTCAAC TGGAAAAGAT TTACGAGTAA TGACAAATAA ACAATTGTCT 
GGACATTTGA TAAGTCTTTG GATAGCTGAT AATCAACAGG CTAATGATTT GTTAATTAGG 
TGCCTGCCCC GTGGTCTATT GAACTATATG GAGTCAGACG AAAAAGTTCC AGTAAACGAG 
AAAGATCTTC TCATTGTTCG AAATAACTTT GATGCAGCAT CAAATGAAAC TAAGCAGAAT 
GCGATGAAGG AGAAGTTTGA CCAGCTTCGA GTTACTGCTG AAGCTGGTTT GGAAAGATTT 
GTGCAGCACT GGGATCTCGA ACAAAAACTC AATTTTCTTC CAAAACGAGT TATTGATGAG 
AAACAACAAC AAAGACAGCA ACCGGTTGTC CTGAGAAAAC GAAGAAATCG AGTACGGCCA 
AATGTTAATT GGAAACTATT TGCATATCAA TTCTCGAAGG ATCACTCTCA GGCAGATTTA 
ATTTGGAATG AGAAGACGCG AGAAGAGTTC CGTCATGCAA TGGATACAGA AACACGAGCT 
CTTATCAGTG AAAAAGAACA AGCACCAACT GGATTGCCGA TTGCTTGGAA TCATACTGAG 
TTCCAAATCA GATATCCATC ACTTTTAGAA GAAATTAAAA TTGGAGATTA TTACCTTCGA 
TTGCTTCTGA TTGAGGCAGA CGAAAATGCG ACTCCGATTC ATAATCCGCT GGAGTTCTTC 
AACAACGTCT ATCATCGTTT CCTACTTTCC ACTAAAGTTG ACATGAAATG TCTGTGCCTT 
CGTGCAATGG CTATCACCTA TTCTCGACAT CATATGACAA TCGGAGCATT TCAGGATTCA 
AAGTATTTCG TAGAAATGCT TCAAAAATGC ATAAATCCGT TGGAAAGAGA TCATCTTGTG 
CTTCTGTTAT CGAAACTAGC ACTGAATAAG GATAATGTTC GAGAACTTAT AATATCCAAT 
ATTCTTCCAC TTTTGGTAGA TCTTTGTGTT CTTGCACATT TACACGTGCA GCGAGCAAAA 
GTTCAAAATC AGACAAATGT GATTGAGGCA AGTGCCGAAC AAATGGCTGA AGGAGGTTCT 
GAAGAATGGT ATTATCATGA TAAGGATGCT AAACAAGTCG GACCATTGTC ATTTGAGAAA 
ATGAAGTCTC TTTACACGGA GAAGACTATT TTTGAGAAAT CTCAAATTTG GGCAGCTGGA 
ATGGACAAGT GGATGAGTTT GGCTGCAGTT CCACAATTCA GATGGACTGT TTGTCAGCAA 
AAAGATCAGC CGAACGAGAT TAATACAGGA AAAGGACCAT TCCAGGCAAC TGTCATGAAC 
TTCACAGATC TCAGTGTTCT TTGTTTAGAT ACTATTCTTC AGATGTGCGA ATTTTTCCCA 
TCAAGGGATT CTCATGATTG TGTGGTCCGT CCGATGCCAT CTGTCAAGAA GCAGCTCACT 
GAGCCAGTAC TCCTTTATCA ACTCGTTCAA CTTCTTCTAA CCTATGAACC ACAGATTGTG 
CAGAGAGTTG CTCTACTACT GTATCTTGTA ATGCAGGATA ACCCATTTCT TCCACGACTT 
TATCTTTCTG GTGTCTTCTA TTTTATTCTT ATGTACAATG GATCAAATGT TCTTCCGATT 
GCTCGATTCC TTCACTATAC CCACATGAAA CAAGCATTCC GTTCTACACT TCCACAATTT 
GAGGGACAAC GGCAATCAGT TCTTGCCACT CTTCTTCCAG AAGCCGCCAC ATTCTATTTG 
GAACAATATG GACCTGAAAA GTATGCCGAA GTGTTCCTTG GAGAATTCGA TAATCCCGAA 
ATCATTTGGA ATACGGCGAT GCGTCGACAT TTGATTGAAC GAATCGCTGT TCATGTTGCA 
GATTTCTCGC ATCGATTAAC ATCAAATGTT CGAGCTTTAT ATCAATTCTG TCCAATTCCT 
TTGATCGATT ATCCAGAACT TGCTCAGGAA CTCTTCTGTC ATGTTTATTA TCTTCGACAC 
TTGTGTAATA AGCAAAGATT CCCTGATTGG GAAATTAGGG ATCCGATTCC GTTCCTTCGA 
TGTTGCCTTG CCACATGGTA TAATGAGCTA GAGAAGAAGC CGGCGACGAT GTCAGTCGAT 
CTTGCAAGAG AGATTTTATC AGTTGATTTG ACTAACGAAG AACACCGAAA ACCTGCATTC 
ATACGTCGTC AATATTACAA ACTCGCTGCT AAATACCATC CTGACAAGAA TCCAGAAGGA 
AGAGAAATGT TCGAACGAAT AAATGCAGCT TATGAACTAT TGAGCAGTGA AACTGCAAAT 
AATTCAGGAA TGCCGGATTC GCATCGAATT GTGCTTTGTT TACAGGCACA ATCCATCATT 
TATAGTAGAT ATAGTCAAGA ATTATCCGAA TACAAATACG CTGGTTACTC TCAACTCATT 
AAAACTATCG ATTTGGAAGC AAAAGACGAA GCATTGTTCA TAAAAGGAGG TGGAGACCTG 
TTGTCAGCTG CTATTGAACT TGCTAATTAT ACACTTATTA GTAGTGCTTT GAATGCTGAA 
CAATTGAGAA GAGATAATGG ATTAGAGGCA TTAGTAACTG CATTTGATCG ATGTGTTCCA 
ATGGTAACAA TGAGTTCATT ACCTGATGAT ATGGCTGTTC GAGTTTGTAT TCATGTTTGT 
GATTGTTTTG CCACTGCTGC AACATTTGAA GCGTGTAGGC AGCGACTGAT GGAGATGCCA 
TCCATATTTG GAGCCCTCAC TAGATTACTT CAATTTTCGA ACTTACCCCG TCTCTCCACT 
GCAGCAGCTC AATGTATTCG TGCAATGGCA GTTGACACAC TTCTTCAATT CCAACTTTTC 
CAAACTGGTG TTCTATGGCA ATTGGTACCA CATCTCTTTC ATTTCGATTA TACATTGGAT 
GAAGGAGGTG TTCAACATTC AGAGGATTCT AATAAACAAT CACTCGCTAA TTCATTAGCT 
CGTTCTTCGT GTGAAGCTCT TGCAGCTCTG GCTGGATTCC GTGAGAATAC ACCGGATAAT 
GATGGAGTTC AGGCAAGCCT TCGAGCTCTA CTTACTCCTT ACATCTGTCG ATGCATGAAG 
CTTGAAACAA ATGATATGGT TCTGAAAACG CTGAATAGTA ATATGGAAAA TCCATATATG 
ATCTGGGATA ATGGAACAAG AGCTGAAGTT CTCGAATTTG TGGAGAGACA TCGAACTTCT 
AATGAGCCTA CGAGTGAGCT GTACGGAGCC GAATTCGAGT TATCTGTGCA CAAAAATGAG 
CTTATCGTTG GAGACATATT CATCAGAATC TACAATGAAC AGCCAACATT TGCTCTTCAC 
GAACCCAAAA AAGTTGCAAT CGATCTTTTG GATTACATCA AAAAACACTC GGCAGAACTA 
ACCGGAGCTC CAAAACCTAA AGCAATTTCG GATGATTTGA TTGAAATTGA CTGGGGAAAT 
GGATCACAGG CTAATGGAAA CTCATTGGAC ACAGAAACAA AAGTTCTCAT GACAATGACT 
GCATTAGCTA ATTTAGTTTC AGCGAATCCA GGTGCACTAT TTGCAAACAA AAAGGGTGTT 
GAAATTCTAC TAATTGGAAA CTTCCCACTT CTTATCACTT ATCTACGTTG TCGAAAACAT 
CCGAAGCTTC AAATTGCTGC ACTTCAAGTC ATTCTGTTGG CTGCAGCTAA TAAGGAATGT 
GTAACTGATT TGGCCACGTG TAATGTCACT ACAACACTTT TCACACTTTT ACGTGATCAG 
CCGAAGATGA TAGCAAGAGT ATTAGATGTT CTGATTGCAC TTTCATCAAA TGGACAAATT 
GGAAAAGAAA TGTTAGAACA TGGAGGACTT ATGTATATTC TATCAATTCT TTGTTTGACA 
AATTCTGATC AAGGCCAAAG ACTGCAGGCA GCCGAGCTTT TGGCCAAACT TCAAGCTGAT 
AAGCTTACTG GGCCAAGATG GACACGATTT ATTATCAAGT TTTTGCCAGA AATATTTGCA 
GATTCATTGA GAGATTCGCC CAATACAGCC CTTCAAATGT TCGATTCATC AAGCGAAAAT 
CCAGAGCTTA TTTGGAATGA AGTAACGCGT CAAAAAGTGA AAGGAATCAT TGATACAGAA 
GTCGGAAAAC TCTATGAACA ACACATCAAA GATCCAACTG TCAAATGGAA TACGACTTCA 
TTGGCTGACA AGAGTTGTGC CTATGGTGAT AGTATTAATG GTGAACTTGT TGTTGGAGGT 
GTTTTTGTAC GATTATACGT CGAAAATCCA GGATTCAATG TCAGACATCC AAAATTATTC 
TCAACAGAGC TCATCGAAAA AGTGCTTGAA CTGATGAGTC GTCCAACTCC AGAACTCGAT 
TTGGTCACAA AAGCCTTTGT TGAACTGGTC CGTCATCATC CGAATACTGC TGATCAATTG 
CCATCTCAAG GATATCTACC ACAGTTCTGC ACTGCAATGT GTCTTCAAAA CACATCTGCG 
TCTCGTTCAG CAATTCTAAT TCTTCAAGAA TTGAGCGAAA ATCAATTCTG TTGTGATGCT 
CTTTCTCAGC TGCCATGTAT TGATGGAATA ATGAAATCAA TGAAGAATCA ACCAAGTTTG 
ATGAGAGAAT CGGCGCACGC TTTAAAATGT CTCATGAAGA GAAATACAGG AGAATTGGCT 
CAACAAATGC TCTCATGTGG AATGGTTCCA TATTTGTTAC AAGTTTTGGA TAGTTCAATG 
AATGGAGTAT CAAACGGAGC AGCTGCTCGT GCCGAGATTG TTGACGCATT AAAAAGCGCA 
ATTTTAGATC TGAAAGTTGG TCAGAAGATT GCTGAGATTT TAGACAAGAG TCCCGTCTGG 
GCACAGTTTA AAGATCAACG ACATGATTTA TTCTTGCCAG AAGCGAGAAC TCAAGCTATT 
ACAGGAGGTC CAACTGGTGT TGCTGGATAT CTAACAGAAG GAATGTTCAA TCCACCACCA 
ATGAGCAATC AGCCACCACC AATGCATCAA ACATCTGGAG ATTTCCAACC ACCACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rme-8 ( NM_059821.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492222.2 Caenorhabditis elegans Receptor Mediated Endocytosis (rme-8), partial mRNA.

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CloneID	OCa335309
Clone ID Related Accession (Same CDS sequence)	NM_059821.5 , NM_059821.4
Accession Version	NM_059821.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Receptor Mediated Endocytosis
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_059821.3. COMPLETENESS: incomplete on both ends.

ATGGCTCAAT TTTGCTCGGA AAACCGAGAC ATTGCATGCT ATTTGGTCAC AAAACACTCA 
TGGAAGGGAA AGTACAAACG GGTGTTTTCT ATCGGGACAC TGGCCATCAC CACTTACAAT 
CCTAATACCT TGGAGATTAC CAATCAATGG CTCTACGAGG ACTTCTTATC AGTGAAACCA 
CTTCGAAATG GAGGTTCAAA TGATTCAAAA CAGGATGAAT ACAAGATTCA TGTTAGGAAT 
CGTGGAAAAA ACAATGATAT GCGTTTTTCA TCTGATTATA CCACCGATAT ACTTACACAT 
TGTCTGCAAT TCAGCACCAA GTTCGCTGAT AAGAACTTTG AACCTTTGAC GGTAACTGCA 
TTCAAACAAA GTTGGAGTGA TCGTCGAATT CCCGTAACTC TACGAGCAAA TGCTTCTTGT 
TTGGAGCAAA TTGATAATCG TGGAGTCGTC GTTCAAAGTT ATCCGTACAA AAATATTCGA 
AGTATTGGAA AAGTGTCCGA TTGTCCCGGT GGTTTTGTGG TTGATGTGGG AGAACATAGA 
CGGAGGCACA TGTTCGCTTC TTCAAACAAC GAAGAACTAG TGAAAGAGAT TCGTCGTTTA 
GCATCTGATA ATATCGGAAT TATCGTTCCA ATTGCTAAAG AGCAGCTAAC ACTCGAAGAT 
TTTATGAGAA CTCGACTCGG ATTGTGCAGT CGAGATGAAG AGCTCACTTC ATATGCTGAA 
TTCAAAATTT CCAAAATTAC TCGAAGAAAT GAGATGCCTG TTCGACGGCT TCTATGTCTC 
AGTGAGACGT GTATTATTGA AAGAGACTTG GCTACTTATG CAGTGATTTG TGCAACTCCA 
CTGAAACATA TCGTTTGCCT TGTTCGAAGT GAAAAAGATC CCCAACAATT CATTGTTGAA 
TACGAAAATG GAGATGGGCG AGCATATGTT GCTGCTGAAA GAGATTTGAT CTTAGCCTCA 
CTCTTAGATG GAATTCGTGC TTCTGGAAAT AATGAAGTGT TCGTTTGTGG ACACAGATTT 
GAAAGGAATT TGAGAGTGAT CCCATTCTCA ACGAATTTGG ATGAAGATAG TGAATCTCAA 
TGTATGAAGC ATATTATTGC ACCTCCACCT GGACTTCGTC GTTGTGATTT GATCCGTCGC 
TTCAACGCAA ATGTTCCATA TTCTGGTCTT CGATTTTCAA AGTCGCACGA AGGATTCTTC 
TCAGAAAACA AAGGAAAAGT TATTGTAAAT GCAATTGAGG CAGTTCTAAT GGAGAATTAT 
ACAAAAGACG ATAAGGAGTA CAAGCACAAA ACTGAAGCTC AGTTACAGTG TTTACGAAGA 
CTTTTTGCGT CGAAAAGTGG ATTCCAGGCA TTTACAGAAG TTAATGGTGT TCGTGAGAAG 
CTTGGGAGTC TTGTGGTTCG AGTTCTATCT TGGAAATCTG AATCAATTGA TCATTCGACG 
GTTGAAGCTC TTTGTGCATT GATGTATCCA ATGCATGATC AATATGAACT TAGAATAGAG 
CAACTGAACA AGCAGAGTCT GATGAGCAGC CCAAAATTTG TGGAGAACTT ACTCGATTTG 
ATTGTTCTTC ACGTGGATCG TTCAACTGGA TGGCTTGTGA TTGCATCAAT GCTCAATTTC 
CTCACATTTT CCGTTTGCTC TCCGTACTCT GAAACCACCG CTGGAGATAC TTTCGATCAT 
ATTCTGAAAC TCGTATCTCT CCGAGGGCGC AGCTTTTTCC GTTTATTCCA ATGTCCCAGT 
ATGACAATTG TTAAAGGTGC TGGAATGGTT ATGAGAGCAA TTATCGAAGA AGCTGATGTT 
GAAACTTCAA AATCAATGCA AATGTTAGCA CTATCAGAAG GAGCATTCTT GACACATTTG 
TTCATGTCAT TATTGTCAAC TGGAAAAGAT TTACGAGTAA TGACAAATAA ACAATTGTCT 
GGACATTTGA TAAGTCTTTG GATAGCTGAT AATCAACAGG CTAATGATTT GTTAATTAGG 
TGCCTGCCCC GTGGTCTATT GAACTATATG GAGTCAGACG AAAAAGTTCC AGTAAACGAG 
AAAGATCTTC TCATTGTTCG AAATAACTTT GATGCAGCAT CAAATGAAAC TAAGCAGAAT 
GCGATGAAGG AGAAGTTTGA CCAGCTTCGA GTTACTGCTG AAGCTGGTTT GGAAAGATTT 
GTGCAGCACT GGGATCTCGA ACAAAAACTC AATTTTCTTC CAAAACGAGT TATTGATGAG 
AAACAACAAC AAAGACAGCA ACCGGTTGTC CTGAGAAAAC GAAGAAATCG AGTACGGCCA 
AATGTTAATT GGAAACTATT TGCATATCAA TTCTCGAAGG ATCACTCTCA GGCAGATTTA 
ATTTGGAATG AGAAGACGCG AGAAGAGTTC CGTCATGCAA TGGATACAGA AACACGAGCT 
CTTATCAGTG AAAAAGAACA AGCACCAACT GGATTGCCGA TTGCTTGGAA TCATACTGAG 
TTCCAAATCA GATATCCATC ACTTTTAGAA GAAATTAAAA TTGGAGATTA TTACCTTCGA 
TTGCTTCTGA TTGAGGCAGA CGAAAATGCG ACTCCGATTC ATAATCCGCT GGAGTTCTTC 
AACAACGTCT ATCATCGTTT CCTACTTTCC ACTAAAGTTG ACATGAAATG TCTGTGCCTT 
CGTGCAATGG CTATCACCTA TTCTCGACAT CATATGACAA TCGGAGCATT TCAGGATTCA 
AAGTATTTCG TAGAAATGCT TCAAAAATGC ATAAATCCGT TGGAAAGAGA TCATCTTGTG 
CTTCTGTTAT CGAAACTAGC ACTGAATAAG GATAATGTTC GAGAACTTAT AATATCCAAT 
ATTCTTCCAC TTTTGGTAGA TCTTTGTGTT CTTGCACATT TACACGTGCA GCGAGCAAAA 
GTTCAAAATC AGACAAATGT GATTGAGGCA AGTGCCGAAC AAATGGCTGA AGGAGGTTCT 
GAAGAATGGT ATTATCATGA TAAGGATGCT AAACAAGTCG GACCATTGTC ATTTGAGAAA 
ATGAAGTCTC TTTACACGGA GAAGACTATT TTTGAGAAAT CTCAAATTTG GGCAGCTGGA 
ATGGACAAGT GGATGAGTTT GGCTGCAGTT CCACAATTCA GATGGACTGT TTGTCAGCAA 
AAAGATCAGC CGAACGAGAT TAATACAGGA AAAGGACCAT TCCAGGCAAC TGTCATGAAC 
TTCACAGATC TCAGTGTTCT TTGTTTAGAT ACTATTCTTC AGATGTGCGA ATTTTTCCCA 
TCAAGGGATT CTCATGATTG TGTGGTCCGT CCGATGCCAT CTGTCAAGAA GCAGCTCACT 
GAGCCAGTAC TCCTTTATCA ACTCGTTCAA CTTCTTCTAA CCTATGAACC ACAGATTGTG 
CAGAGAGTTG CTCTACTACT GTATCTTGTA ATGCAGGATA ACCCATTTCT TCCACGACTT 
TATCTTTCTG GTGTCTTCTA TTTTATTCTT ATGTACAATG GATCAAATGT TCTTCCGATT 
GCTCGATTCC TTCACTATAC CCACATGAAA CAAGCATTCC GTTCTACACT TCCACAATTT 
GAGGGACAAC GGCAATCAGT TCTTGCCACT CTTCTTCCAG AAGCCGCCAC ATTCTATTTG 
GAACAATATG GACCTGAAAA GTATGCCGAA GTGTTCCTTG GAGAATTCGA TAATCCCGAA 
ATCATTTGGA ATACGGCGAT GCGTCGACAT TTGATTGAAC GAATCGCTGT TCATGTTGCA 
GATTTCTCGC ATCGATTAAC ATCAAATGTT CGAGCTTTAT ATCAATTCTG TCCAATTCCT 
TTGATCGATT ATCCAGAACT TGCTCAGGAA CTCTTCTGTC ATGTTTATTA TCTTCGACAC 
TTGTGTAATA AGCAAAGATT CCCTGATTGG GAAATTAGGG ATCCGATTCC GTTCCTTCGA 
TGTTGCCTTG CCACATGGTA TAATGAGCTA GAGAAGAAGC CGGCGACGAT GTCAGTCGAT 
CTTGCAAGAG AGATTTTATC AGTTGATTTG ACTAACGAAG AACACCGAAA ACCTGCATTC 
ATACGTCGTC AATATTACAA ACTCGCTGCT AAATACCATC CTGACAAGAA TCCAGAAGGA 
AGAGAAATGT TCGAACGAAT AAATGCAGCT TATGAACTAT TGAGCAGTGA AACTGCAAAT 
AATTCAGGAA TGCCGGATTC GCATCGAATT GTGCTTTGTT TACAGGCACA ATCCATCATT 
TATAGTAGAT ATAGTCAAGA ATTATCCGAA TACAAATACG CTGGTTACTC TCAACTCATT 
AAAACTATCG ATTTGGAAGC AAAAGACGAA GCATTGTTCA TAAAAGGAGG TGGAGACCTG 
TTGTCAGCTG CTATTGAACT TGCTAATTAT ACACTTATTA GTAGTGCTTT GAATGCTGAA 
CAATTGAGAA GAGATAATGG ATTAGAGGCA TTAGTAACTG CATTTGATCG ATGTGTTCCA 
ATGGTAACAA TGAGTTCATT ACCTGATGAT ATGGCTGTTC GAGTTTGTAT TCATGTTTGT 
GATTGTTTTG CCACTGCTGC AACATTTGAA GCGTGTAGGC AGCGACTGAT GGAGATGCCA 
TCCATATTTG GAGCCCTCAC TAGATTACTT CAATTTTCGA ACTTACCCCG TCTCTCCACT 
GCAGCAGCTC AATGTATTCG TGCAATGGCA GTTGACACAC TTCTTCAATT CCAACTTTTC 
CAAACTGGTG TTCTATGGCA ATTGGTACCA CATCTCTTTC ATTTCGATTA TACATTGGAT 
GAAGGAGGTG TTCAACATTC AGAGGATTCT AATAAACAAT CACTCGCTAA TTCATTAGCT 
CGTTCTTCGT GTGAAGCTCT TGCAGCTCTG GCTGGATTCC GTGAGAATAC ACCGGATAAT 
GATGGAGTTC AGGCAAGCCT TCGAGCTCTA CTTACTCCTT ACATCTGTCG ATGCATGAAG 
CTTGAAACAA ATGATATGGT TCTGAAAACG CTGAATAGTA ATATGGAAAA TCCATATATG 
ATCTGGGATA ATGGAACAAG AGCTGAAGTT CTCGAATTTG TGGAGAGACA TCGAACTTCT 
AATGAGCCTA CGAGTGAGCT GTACGGAGCC GAATTCGAGT TATCTGTGCA CAAAAATGAG 
CTTATCGTTG GAGACATATT CATCAGAATC TACAATGAAC AGCCAACATT TGCTCTTCAC 
GAACCCAAAA AAGTTGCAAT CGATCTTTTG GATTACATCA AAAAACACTC GGCAGAACTA 
ACCGGAGCTC CAAAACCTAA AGCAATTTCG GATGATTTGA TTGAAATTGA CTGGGGAAAT 
GGATCACAGG CTAATGGAAA CTCATTGGAC ACAGAAACAA AAGTTCTCAT GACAATGACT 
GCATTAGCTA ATTTAGTTTC AGCGAATCCA GGTGCACTAT TTGCAAACAA AAAGGGTGTT 
GAAATTCTAC TAATTGGAAA CTTCCCACTT CTTATCACTT ATCTACGTTG TCGAAAACAT 
CCGAAGCTTC AAATTGCTGC ACTTCAAGTC ATTCTGTTGG CTGCAGCTAA TAAGGAATGT 
GTAACTGATT TGGCCACGTG TAATGTCACT ACAACACTTT TCACACTTTT ACGTGATCAG 
CCGAAGATGA TAGCAAGAGT ATTAGATGTT CTGATTGCAC TTTCATCAAA TGGACAAATT 
GGAAAAGAAA TGTTAGAACA TGGAGGACTT ATGTATATTC TATCAATTCT TTGTTTGACA 
AATTCTGATC AAGGCCAAAG ACTGCAGGCA GCCGAGCTTT TGGCCAAACT TCAAGCTGAT 
AAGCTTACTG GGCCAAGATG GACACGATTT ATTATCAAGT TTTTGCCAGA AATATTTGCA 
GATTCATTGA GAGATTCGCC CAATACAGCC CTTCAAATGT TCGATTCATC AAGCGAAAAT 
CCAGAGCTTA TTTGGAATGA AGTAACGCGT CAAAAAGTGA AAGGAATCAT TGATACAGAA 
GTCGGAAAAC TCTATGAACA ACACATCAAA GATCCAACTG TCAAATGGAA TACGACTTCA 
TTGGCTGACA AGAGTTGTGC CTATGGTGAT AGTATTAATG GTGAACTTGT TGTTGGAGGT 
GTTTTTGTAC GATTATACGT CGAAAATCCA GGATTCAATG TCAGACATCC AAAATTATTC 
TCAACAGAGC TCATCGAAAA AGTGCTTGAA CTGATGAGTC GTCCAACTCC AGAACTCGAT 
TTGGTCACAA AAGCCTTTGT TGAACTGGTC CGTCATCATC CGAATACTGC TGATCAATTG 
CCATCTCAAG GATATCTACC ACAGTTCTGC ACTGCAATGT GTCTTCAAAA CACATCTGCG 
TCTCGTTCAG CAATTCTAAT TCTTCAAGAA TTGAGCGAAA ATCAATTCTG TTGTGATGCT 
CTTTCTCAGC TGCCATGTAT TGATGGAATA ATGAAATCAA TGAAGAATCA ACCAAGTTTG 
ATGAGAGAAT CGGCGCACGC TTTAAAATGT CTCATGAAGA GAAATACAGG AGAATTGGCT 
CAACAAATGC TCTCATGTGG AATGGTTCCA TATTTGTTAC AAGTTTTGGA TAGTTCAATG 
AATGGAGTAT CAAACGGAGC AGCTGCTCGT GCCGAGATTG TTGACGCATT AAAAAGCGCA 
ATTTTAGATC TGAAAGTTGG TCAGAAGATT GCTGAGATTT TAGACAAGAG TCCCGTCTGG 
GCACAGTTTA AAGATCAACG ACATGATTTA TTCTTGCCAG AAGCGAGAAC TCAAGCTATT 
ACAGGAGGTC CAACTGGTGT TGCTGGATAT CTAACAGAAG GAATGTTCAA TCCACCACCA 
ATGAGCAATC AGCCACCACC AATGCATCAA ACATCTGGAG ATTTCCAACC ACCACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492222.2
CDS	1..6840
Translation	MAQFCSENRDIACYLVTKHSWKGKYKRVFSIGTLAITTYNPNTLEITNQWLYEDFLSVKPLRNGGSNDSKQDEYKIHVRNRGKNNDMRFSSDYTTDILTHCLQFSTKFADKNFEPLTVTAFKQSWSDRRIPVTLRANASCLEQIDNRGVVVQSYPYKNIRSIGKVSDCPGGFVVDVGEHRRRHMFASSNNEELVKEIRRLASDNIGIIVPIAKEQLTLEDFMRTRLGLCSRDEELTSYAEFKISKITRRNEMPVRRLLCLSETCIIERDLATYAVICATPLKHIVCLVRSEKDPQQFIVEYENGDGRAYVAAERDLILASLLDGIRASGNNEVFVCGHRFERNLRVIPFSTNLDEDSESQCMKHIIAPPPGLRRCDLIRRFNANVPYSGLRFSKSHEGFFSENKGKVIVNAIEAVLMENYTKDDKEYKHKTEAQLQCLRRLFASKSGFQAFTEVNGVREKLGSLVVRVLSWKSESIDHSTVEALCALMYPMHDQYELRIEQLNKQSLMSSPKFVENLLDLIVLHVDRSTGWLVIASMLNFLTFSVCSPYSETTAGDTFDHILKLVSLRGRSFFRLFQCPSMTIVKGAGMVMRAIIEEADVETSKSMQMLALSEGAFLTHLFMSLLSTGKDLRVMTNKQLSGHLISLWIADNQQANDLLIRCLPRGLLNYMESDEKVPVNEKDLLIVRNNFDAASNETKQNAMKEKFDQLRVTAEAGLERFVQHWDLEQKLNFLPKRVIDEKQQQRQQPVVLRKRRNRVRPNVNWKLFAYQFSKDHSQADLIWNEKTREEFRHAMDTETRALISEKEQAPTGLPIAWNHTEFQIRYPSLLEEIKIGDYYLRLLLIEADENATPIHNPLEFFNNVYHRFLLSTKVDMKCLCLRAMAITYSRHHMTIGAFQDSKYFVEMLQKCINPLERDHLVLLLSKLALNKDNVRELIISNILPLLVDLCVLAHLHVQRAKVQNQTNVIEASAEQMAEGGSEEWYYHDKDAKQVGPLSFEKMKSLYTEKTIFEKSQIWAAGMDKWMSLAAVPQFRWTVCQQKDQPNEINTGKGPFQATVMNFTDLSVLCLDTILQMCEFFPSRDSHDCVVRPMPSVKKQLTEPVLLYQLVQLLLTYEPQIVQRVALLLYLVMQDNPFLPRLYLSGVFYFILMYNGSNVLPIARFLHYTHMKQAFRSTLPQFEGQRQSVLATLLPEAATFYLEQYGPEKYAEVFLGEFDNPEIIWNTAMRRHLIERIAVHVADFSHRLTSNVRALYQFCPIPLIDYPELAQELFCHVYYLRHLCNKQRFPDWEIRDPIPFLRCCLATWYNELEKKPATMSVDLAREILSVDLTNEEHRKPAFIRRQYYKLAAKYHPDKNPEGREMFERINAAYELLSSETANNSGMPDSHRIVLCLQAQSIIYSRYSQELSEYKYAGYSQLIKTIDLEAKDEALFIKGGGDLLSAAIELANYTLISSALNAEQLRRDNGLEALVTAFDRCVPMVTMSSLPDDMAVRVCIHVCDCFATAATFEACRQRLMEMPSIFGALTRLLQFSNLPRLSTAAAQCIRAMAVDTLLQFQLFQTGVLWQLVPHLFHFDYTLDEGGVQHSEDSNKQSLANSLARSSCEALAALAGFRENTPDNDGVQASLRALLTPYICRCMKLETNDMVLKTLNSNMENPYMIWDNGTRAEVLEFVERHRTSNEPTSELYGAEFELSVHKNELIVGDIFIRIYNEQPTFALHEPKKVAIDLLDYIKKHSAELTGAPKPKAISDDLIEIDWGNGSQANGNSLDTETKVLMTMTALANLVSANPGALFANKKGVEILLIGNFPLLITYLRCRKHPKLQIAALQVILLAAANKECVTDLATCNVTTTLFTLLRDQPKMIARVLDVLIALSSNGQIGKEMLEHGGLMYILSILCLTNSDQGQRLQAAELLAKLQADKLTGPRWTRFIIKFLPEIFADSLRDSPNTALQMFDSSSENPELIWNEVTRQKVKGIIDTEVGKLYEQHIKDPTVKWNTTSLADKSCAYGDSINGELVVGGVFVRLYVENPGFNVRHPKLFSTELIEKVLELMSRPTPELDLVTKAFVELVRHHPNTADQLPSQGYLPQFCTAMCLQNTSASRSAILILQELSENQFCCDALSQLPCIDGIMKSMKNQPSLMRESAHALKCLMKRNTGELAQQMLSCGMVPYLLQVLDSSMNGVSNGAAARAEIVDALKSAILDLKVGQKIAEILDKSPVWAQFKDQRHDLFLPEARTQAITGGPTGVAGYLTEGMFNPPPMSNQPPPMHQTSGDFQPPL

Target ORF information:

RefSeq Version	NM_059821.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Receptor Mediated Endocytosis (rme-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059821.4

ORF Insert Sequence:

ATGGCTCAAT TTTGCTCGGA AAACCGAGAC ATTGCATGCT ATTTGGTCAC AAAACACTCA 
TGGAAGGGAA AGTACAAACG GGTGTTTTCT ATCGGGACAC TGGCCATCAC CACTTACAAT 
CCTAATACCT TGGAGATTAC CAATCAATGG CTCTACGAGG ACTTCTTATC AGTGAAACCA 
CTTCGAAATG GAGGTTCAAA TGATTCAAAA CAGGATGAAT ACAAGATTCA TGTTAGGAAT 
CGTGGAAAAA ACAATGATAT GCGTTTTTCA TCTGATTATA CCACCGATAT ACTTACACAT 
TGTCTGCAAT TCAGCACCAA GTTCGCTGAT AAGAACTTTG AACCTTTGAC GGTAACTGCA 
TTCAAACAAA GTTGGAGTGA TCGTCGAATT CCCGTAACTC TACGAGCAAA TGCTTCTTGT 
TTGGAGCAAA TTGATAATCG TGGAGTCGTC GTTCAAAGTT ATCCGTACAA AAATATTCGA 
AGTATTGGAA AAGTGTCCGA TTGTCCCGGT GGTTTTGTGG TTGATGTGGG AGAACATAGA 
CGGAGGCACA TGTTCGCTTC TTCAAACAAC GAAGAACTAG TGAAAGAGAT TCGTCGTTTA 
GCATCTGATA ATATCGGAAT TATCGTTCCA ATTGCTAAAG AGCAGCTAAC ACTCGAAGAT 
TTTATGAGAA CTCGACTCGG ATTGTGCAGT CGAGATGAAG AGCTCACTTC ATATGCTGAA 
TTCAAAATTT CCAAAATTAC TCGAAGAAAT GAGATGCCTG TTCGACGGCT TCTATGTCTC 
AGTGAGACGT GTATTATTGA AAGAGACTTG GCTACTTATG CAGTGATTTG TGCAACTCCA 
CTGAAACATA TCGTTTGCCT TGTTCGAAGT GAAAAAGATC CCCAACAATT CATTGTTGAA 
TACGAAAATG GAGATGGGCG AGCATATGTT GCTGCTGAAA GAGATTTGAT CTTAGCCTCA 
CTCTTAGATG GAATTCGTGC TTCTGGAAAT AATGAAGTGT TCGTTTGTGG ACACAGATTT 
GAAAGGAATT TGAGAGTGAT CCCATTCTCA ACGAATTTGG ATGAAGATAG TGAATCTCAA 
TGTATGAAGC ATATTATTGC ACCTCCACCT GGACTTCGTC GTTGTGATTT GATCCGTCGC 
TTCAACGCAA ATGTTCCATA TTCTGGTCTT CGATTTTCAA AGTCGCACGA AGGATTCTTC 
TCAGAAAACA AAGGAAAAGT TATTGTAAAT GCAATTGAGG CAGTTCTAAT GGAGAATTAT 
ACAAAAGACG ATAAGGAGTA CAAGCACAAA ACTGAAGCTC AGTTACAGTG TTTACGAAGA 
CTTTTTGCGT CGAAAAGTGG ATTCCAGGCA TTTACAGAAG TTAATGGTGT TCGTGAGAAG 
CTTGGGAGTC TTGTGGTTCG AGTTCTATCT TGGAAATCTG AATCAATTGA TCATTCGACG 
GTTGAAGCTC TTTGTGCATT GATGTATCCA ATGCATGATC AATATGAACT TAGAATAGAG 
CAACTGAACA AGCAGAGTCT GATGAGCAGC CCAAAATTTG TGGAGAACTT ACTCGATTTG 
ATTGTTCTTC ACGTGGATCG TTCAACTGGA TGGCTTGTGA TTGCATCAAT GCTCAATTTC 
CTCACATTTT CCGTTTGCTC TCCGTACTCT GAAACCACCG CTGGAGATAC TTTCGATCAT 
ATTCTGAAAC TCGTATCTCT CCGAGGGCGC AGCTTTTTCC GTTTATTCCA ATGTCCCAGT 
ATGACAATTG TTAAAGGTGC TGGAATGGTT ATGAGAGCAA TTATCGAAGA AGCTGATGTT 
GAAACTTCAA AATCAATGCA AATGTTAGCA CTATCAGAAG GAGCATTCTT GACACATTTG 
TTCATGTCAT TATTGTCAAC TGGAAAAGAT TTACGAGTAA TGACAAATAA ACAATTGTCT 
GGACATTTGA TAAGTCTTTG GATAGCTGAT AATCAACAGG CTAATGATTT GTTAATTAGG 
TGCCTGCCCC GTGGTCTATT GAACTATATG GAGTCAGACG AAAAAGTTCC AGTAAACGAG 
AAAGATCTTC TCATTGTTCG AAATAACTTT GATGCAGCAT CAAATGAAAC TAAGCAGAAT 
GCGATGAAGG AGAAGTTTGA CCAGCTTCGA GTTACTGCTG AAGCTGGTTT GGAAAGATTT 
GTGCAGCACT GGGATCTCGA ACAAAAACTC AATTTTCTTC CAAAACGAGT TATTGATGAG 
AAACAACAAC AAAGACAGCA ACCGGTTGTC CTGAGAAAAC GAAGAAATCG AGTACGGCCA 
AATGTTAATT GGAAACTATT TGCATATCAA TTCTCGAAGG ATCACTCTCA GGCAGATTTA 
ATTTGGAATG AGAAGACGCG AGAAGAGTTC CGTCATGCAA TGGATACAGA AACACGAGCT 
CTTATCAGTG AAAAAGAACA AGCACCAACT GGATTGCCGA TTGCTTGGAA TCATACTGAG 
TTCCAAATCA GATATCCATC ACTTTTAGAA GAAATTAAAA TTGGAGATTA TTACCTTCGA 
TTGCTTCTGA TTGAGGCAGA CGAAAATGCG ACTCCGATTC ATAATCCGCT GGAGTTCTTC 
AACAACGTCT ATCATCGTTT CCTACTTTCC ACTAAAGTTG ACATGAAATG TCTGTGCCTT 
CGTGCAATGG CTATCACCTA TTCTCGACAT CATATGACAA TCGGAGCATT TCAGGATTCA 
AAGTATTTCG TAGAAATGCT TCAAAAATGC ATAAATCCGT TGGAAAGAGA TCATCTTGTG 
CTTCTGTTAT CGAAACTAGC ACTGAATAAG GATAATGTTC GAGAACTTAT AATATCCAAT 
ATTCTTCCAC TTTTGGTAGA TCTTTGTGTT CTTGCACATT TACACGTGCA GCGAGCAAAA 
GTTCAAAATC AGACAAATGT GATTGAGGCA AGTGCCGAAC AAATGGCTGA AGGAGGTTCT 
GAAGAATGGT ATTATCATGA TAAGGATGCT AAACAAGTCG GACCATTGTC ATTTGAGAAA 
ATGAAGTCTC TTTACACGGA GAAGACTATT TTTGAGAAAT CTCAAATTTG GGCAGCTGGA 
ATGGACAAGT GGATGAGTTT GGCTGCAGTT CCACAATTCA GATGGACTGT TTGTCAGCAA 
AAAGATCAGC CGAACGAGAT TAATACAGGA AAAGGACCAT TCCAGGCAAC TGTCATGAAC 
TTCACAGATC TCAGTGTTCT TTGTTTAGAT ACTATTCTTC AGATGTGCGA ATTTTTCCCA 
TCAAGGGATT CTCATGATTG TGTGGTCCGT CCGATGCCAT CTGTCAAGAA GCAGCTCACT 
GAGCCAGTAC TCCTTTATCA ACTCGTTCAA CTTCTTCTAA CCTATGAACC ACAGATTGTG 
CAGAGAGTTG CTCTACTACT GTATCTTGTA ATGCAGGATA ACCCATTTCT TCCACGACTT 
TATCTTTCTG GTGTCTTCTA TTTTATTCTT ATGTACAATG GATCAAATGT TCTTCCGATT 
GCTCGATTCC TTCACTATAC CCACATGAAA CAAGCATTCC GTTCTACACT TCCACAATTT 
GAGGGACAAC GGCAATCAGT TCTTGCCACT CTTCTTCCAG AAGCCGCCAC ATTCTATTTG 
GAACAATATG GACCTGAAAA GTATGCCGAA GTGTTCCTTG GAGAATTCGA TAATCCCGAA 
ATCATTTGGA ATACGGCGAT GCGTCGACAT TTGATTGAAC GAATCGCTGT TCATGTTGCA 
GATTTCTCGC ATCGATTAAC ATCAAATGTT CGAGCTTTAT ATCAATTCTG TCCAATTCCT 
TTGATCGATT ATCCAGAACT TGCTCAGGAA CTCTTCTGTC ATGTTTATTA TCTTCGACAC 
TTGTGTAATA AGCAAAGATT CCCTGATTGG GAAATTAGGG ATCCGATTCC GTTCCTTCGA 
TGTTGCCTTG CCACATGGTA TAATGAGCTA GAGAAGAAGC CGGCGACGAT GTCAGTCGAT 
CTTGCAAGAG AGATTTTATC AGTTGATTTG ACTAACGAAG AACACCGAAA ACCTGCATTC 
ATACGTCGTC AATATTACAA ACTCGCTGCT AAATACCATC CTGACAAGAA TCCAGAAGGA 
AGAGAAATGT TCGAACGAAT AAATGCAGCT TATGAACTAT TGAGCAGTGA AACTGCAAAT 
AATTCAGGAA TGCCGGATTC GCATCGAATT GTGCTTTGTT TACAGGCACA ATCCATCATT 
TATAGTAGAT ATAGTCAAGA ATTATCCGAA TACAAATACG CTGGTTACTC TCAACTCATT 
AAAACTATCG ATTTGGAAGC AAAAGACGAA GCATTGTTCA TAAAAGGAGG TGGAGACCTG 
TTGTCAGCTG CTATTGAACT TGCTAATTAT ACACTTATTA GTAGTGCTTT GAATGCTGAA 
CAATTGAGAA GAGATAATGG ATTAGAGGCA TTAGTAACTG CATTTGATCG ATGTGTTCCA 
ATGGTAACAA TGAGTTCATT ACCTGATGAT ATGGCTGTTC GAGTTTGTAT TCATGTTTGT 
GATTGTTTTG CCACTGCTGC AACATTTGAA GCGTGTAGGC AGCGACTGAT GGAGATGCCA 
TCCATATTTG GAGCCCTCAC TAGATTACTT CAATTTTCGA ACTTACCCCG TCTCTCCACT 
GCAGCAGCTC AATGTATTCG TGCAATGGCA GTTGACACAC TTCTTCAATT CCAACTTTTC 
CAAACTGGTG TTCTATGGCA ATTGGTACCA CATCTCTTTC ATTTCGATTA TACATTGGAT 
GAAGGAGGTG TTCAACATTC AGAGGATTCT AATAAACAAT CACTCGCTAA TTCATTAGCT 
CGTTCTTCGT GTGAAGCTCT TGCAGCTCTG GCTGGATTCC GTGAGAATAC ACCGGATAAT 
GATGGAGTTC AGGCAAGCCT TCGAGCTCTA CTTACTCCTT ACATCTGTCG ATGCATGAAG 
CTTGAAACAA ATGATATGGT TCTGAAAACG CTGAATAGTA ATATGGAAAA TCCATATATG 
ATCTGGGATA ATGGAACAAG AGCTGAAGTT CTCGAATTTG TGGAGAGACA TCGAACTTCT 
AATGAGCCTA CGAGTGAGCT GTACGGAGCC GAATTCGAGT TATCTGTGCA CAAAAATGAG 
CTTATCGTTG GAGACATATT CATCAGAATC TACAATGAAC AGCCAACATT TGCTCTTCAC 
GAACCCAAAA AAGTTGCAAT CGATCTTTTG GATTACATCA AAAAACACTC GGCAGAACTA 
ACCGGAGCTC CAAAACCTAA AGCAATTTCG GATGATTTGA TTGAAATTGA CTGGGGAAAT 
GGATCACAGG CTAATGGAAA CTCATTGGAC ACAGAAACAA AAGTTCTCAT GACAATGACT 
GCATTAGCTA ATTTAGTTTC AGCGAATCCA GGTGCACTAT TTGCAAACAA AAAGGGTGTT 
GAAATTCTAC TAATTGGAAA CTTCCCACTT CTTATCACTT ATCTACGTTG TCGAAAACAT 
CCGAAGCTTC AAATTGCTGC ACTTCAAGTC ATTCTGTTGG CTGCAGCTAA TAAGGAATGT 
GTAACTGATT TGGCCACGTG TAATGTCACT ACAACACTTT TCACACTTTT ACGTGATCAG 
CCGAAGATGA TAGCAAGAGT ATTAGATGTT CTGATTGCAC TTTCATCAAA TGGACAAATT 
GGAAAAGAAA TGTTAGAACA TGGAGGACTT ATGTATATTC TATCAATTCT TTGTTTGACA 
AATTCTGATC AAGGCCAAAG ACTGCAGGCA GCCGAGCTTT TGGCCAAACT TCAAGCTGAT 
AAGCTTACTG GGCCAAGATG GACACGATTT ATTATCAAGT TTTTGCCAGA AATATTTGCA 
GATTCATTGA GAGATTCGCC CAATACAGCC CTTCAAATGT TCGATTCATC AAGCGAAAAT 
CCAGAGCTTA TTTGGAATGA AGTAACGCGT CAAAAAGTGA AAGGAATCAT TGATACAGAA 
GTCGGAAAAC TCTATGAACA ACACATCAAA GATCCAACTG TCAAATGGAA TACGACTTCA 
TTGGCTGACA AGAGTTGTGC CTATGGTGAT AGTATTAATG GTGAACTTGT TGTTGGAGGT 
GTTTTTGTAC GATTATACGT CGAAAATCCA GGATTCAATG TCAGACATCC AAAATTATTC 
TCAACAGAGC TCATCGAAAA AGTGCTTGAA CTGATGAGTC GTCCAACTCC AGAACTCGAT 
TTGGTCACAA AAGCCTTTGT TGAACTGGTC CGTCATCATC CGAATACTGC TGATCAATTG 
CCATCTCAAG GATATCTACC ACAGTTCTGC ACTGCAATGT GTCTTCAAAA CACATCTGCG 
TCTCGTTCAG CAATTCTAAT TCTTCAAGAA TTGAGCGAAA ATCAATTCTG TTGTGATGCT 
CTTTCTCAGC TGCCATGTAT TGATGGAATA ATGAAATCAA TGAAGAATCA ACCAAGTTTG 
ATGAGAGAAT CGGCGCACGC TTTAAAATGT CTCATGAAGA GAAATACAGG AGAATTGGCT 
CAACAAATGC TCTCATGTGG AATGGTTCCA TATTTGTTAC AAGTTTTGGA TAGTTCAATG 
AATGGAGTAT CAAACGGAGC AGCTGCTCGT GCCGAGATTG TTGACGCATT AAAAAGCGCA 
ATTTTAGATC TGAAAGTTGG TCAGAAGATT GCTGAGATTT TAGACAAGAG TCCCGTCTGG 
GCACAGTTTA AAGATCAACG ACATGATTTA TTCTTGCCAG AAGCGAGAAC TCAAGCTATT 
ACAGGAGGTC CAACTGGTGT TGCTGGATAT CTAACAGAAG GAATGTTCAA TCCACCACCA 
ATGAGCAATC AGCCACCACC AATGCATCAA ACATCTGGAG ATTTCCAACC ACCACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rme-8 ( NM_001026224.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021395.1 Caenorhabditis elegans Receptor Mediated Endocytosis (rme-8), partial mRNA.

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CloneID	OCa335310
Clone ID Related Accession (Same CDS sequence)	NM_001026224.3 , NM_001026224.4
Accession Version	NM_001026224.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Receptor Mediated Endocytosis
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_001026224.2. COMPLETENESS: incomplete on both ends.

ATGGCTCAAT TTTGCTCGGA AAACCGAGAC ATTGCATGCT ATTTGGTCAC AAAACACTCA 
TGGAAGGGAA AGTACAAACG GGTGTTTTCT ATCGGGACAC TGGCCATCAC CACTTACAAT 
CCTAATACCT TGGAGATTAC CAATCAATGG CTCTACGAGG ACTTCTTATC AGTGAAACCA 
CTTCGAAATG GAGGTTCAAA TGATTCAAAA CAGGATGAAT ACAAGATTCA TGTTAGGAAT 
CGTGGAAAAA ACAATGATAT GCGTTTTTCA TCTGATTATA CCACCGATAT ACTTACACAT 
TGTCTGCAAT TCAGCACCAA GTTCGCTGAT AAGAACTTTG AACCTTTGAC GGTAACTGCA 
TTCAAACAAA GTTGGAGTGA TCGTCGAATT CCCGTAACTC TACGAGCAAA TGCTTCTTGT 
TTGGAGCAAA TTGATAATCG TGGAGTCGTC GTTCAAAGTT ATCCGTACAA AAATATTCGA 
AGTATTGGAA AAGTGTCCGA TTGTCCCGGT GGTTTTGTGG TTGATGTGGG AGAACATAGA 
CGGAGGCACA TGTTCGCTTC TTCAAACAAC GAAGAACTAG TGAAAGAGAT TCGTCGTTTA 
GCATCTGATA ATATCGGAAT TATCGTTCCA ATTGCTAAAG AGCAGCTAAC ACTCGAAGAT 
TTTATGAGAA CTCGACTCGG ATTGTGCAGT CGAGATGAAG AGCTCACTTC ATATGCTGAA 
TTCAAAATTT CCAAAATTAC TCGAAGAAAT GAGATGCCTG TTCGACGGCT TCTATGTCTC 
AGTGAGACGT GTATTATTGA AAGAGACTTG GCTACTTATG CAGTGATTTG TGCAACTCCA 
CTGAAACATA TCGTTTGCCT TGTTCGAAGT GAAAAAGATC CCCAACAATT CATTGTTGAA 
TACGAAAATG GAGATGGGCG AGCATATGTT GCTGCTGAAA GAGATTTGAT CTTAGCCTCA 
CTCTTAGATG GAATTCGTGC TTCTGGAAAT AATGAAGTGT TCGTTTGTGG ACACAGATTT 
GAAAGGAATT TGAGAGTGAT CCCATTCTCA ACGAATTTGG ATGAAGATAG TGAATCTCAA 
TGTATGAAGC ATATTATTGC ACCTCCACCT GGACTTCGTC GTTGTGATTT GATCCGTCGC 
TTCAACGCAA ATGTTCCATA TTCTGGTCTT CGATTTTCAA AGTCGCACGA AGGATTCTTC 
TCAGAAAACA AAGGAAAAGT TATTGTAAAT GCAATTGAGG CAGTTCTAAT GGAGAATTAT 
ACAAAAGACG ATAAGGAGTA CAAGCACAAA ACTGAAGCTC AGTTACAGTG TTTACGAAGA 
CTTTTTGCGT CGAAAAGTGG ATTCCAGGCA TTTACAGAAG TTAATGGTGT TCGTGAGAAG 
CTTGGGAGTC TTGTGGTTCG AGTTCTATCT TGGAAATCTG AATCAATTGA TCATTCGACG 
GTTGAAGCTC TTTGTGCATT GATGTATCCA ATGCATGATC AATATGAACT TAGAATAGAG 
CAACTGAACA AGCAGAGTCT GATGAGCAGC CCAAAATTTG TGGAGAACTT ACTCGATTTG 
ATTGTTCTTC ACGTGGATCG TTCAACTGGA TGGCTTGTGA TTGCATCAAT GCTCAATTTC 
CTCACATTTT CCGTTTGCTC TCCGTACTCT GAAACCACCG CTGGAGATAC TTTCGATCAT 
ATTCTGAAAC TCGTATCTCT CCGAGGGCGC AGCTTTTTCC GTTTATTCCA ATGTCCCAGT 
ATGACAATTG TTAAAGGTGC TGGAATGGTT ATGAGAGCAA TTATCGAAGA AGCTGATGTT 
GAAACTTCAA AATCAATGCA AATGTTAGCA CTATCAGAAG GAGCATTCTT GACACATTTG 
TTCATGTCAT TATTGTCAAC TGGAAAAGAT TTACGAGTAA TGACAAATAA ACAATTGTCT 
GGACATTTGA TAAGTCTTTG GATAGCTGAT AATCAACAGG CTAATGATTT GTTAATTAGG 
TGCCTGCCCC GTGGTCTATT GAACTATATG GAGTCAGACG AAAAAGTTCC AGTAAACGAG 
AAAGATCTTC TCATTGTTCG AAATAACTTT GATGCAGCAT CAAATGAAAC TAAGCAGAAT 
GCGATGAAGG AGAAGTTTGA CCAGCTTCGA GTTACTGCTG AAGCTGGTTT GGAAAGATTT 
GTGCAGCACT GGGATCTCGA ACAAAAACTC AATTTTCTTC CAAAACGAGT TATTGATGAG 
AAACAACAAC AAAGACAGCA ACCGGTTGTC CTGAGAAAAC GAAGAAATCG AGTACGGCCA 
AATGTTAATT GGAAACTATT TGCATATCAA TTCTCGAAGG ATCACTCTCA GGCAGATTTA 
ATTTGGAATG AGAAGACGCG AGAAGAGTTC CGTCATGCAA TGGATACAGA AACACGAGCT 
CTTATCAGTG AAAAAGAACA AGCACCAACT GGATTGCCGA TTGCTTGGAA TCATACTGAG 
TTCCAAATCA GATATCCATC ACTTTTAGAA GAAATTAAAA TTGGAGATTA TTACCTTCGA 
TTGCTTCTGA TTGAGGCAGA CGAAAATGCG ACTCCGATTC ATAATCCGCT GGAGTTCTTC 
AACAACGTCT ATCATCGTTT CCTACTTTCC ACTAAAGTTG ACATGAAATG TCTGTGCCTT 
CGTGCAATGG CTATCACCTA TTCTCGACAT CATATGACAA TCGGAGCATT TCAGGATTCA 
AAGTATTTCG TAGAAATGCT TCAAAAATGC ATAAATCCGT TGGAAAGAGA TCATCTTGTG 
CTTCTGTTAT CGAAACTAGC ACTGAATAAG GATAATGTTC GAGAACTTAT AATATCCAAT 
ATTCTTCCAC TTTTGGTAGA TCTTTGTGTT CTTGCACATT TACACGTGCA GCGAGCAAAA 
GTTCAAAATC AGACAAATGT GATTGAGGCA AGTGCCGAAC AAATGGCTGA AGGAGGTTCT 
GAAGAATGGT ATTATCATGA TAAGGATGCT AAACAAGTCG GACCATTGTC ATTTGAGAAA 
ATGAAGTCTC TTTACACGGA GAAGACTATT TTTGAGAAAT CTCAAATTTG GGCAGCTGGA 
ATGGACAAGT GGATGAGTTT GGCTGCAGTT CCACAATTCA GATGGACTGT TTGTCAGCAA 
AAAGATCAGC CGAACGAGAT TAATACAGGA AAAGGACCAT TCCAGGCAAC TGTCATGAAC 
TTCACAGATC TCAGTGTTCT TTGTTTAGAT ACTATTCTTC AGATGTGCGA ATTTTTCCCA 
TCAAGGGATT CTCATGATTG TGTGGTCCGT CCGATGCCAT CTGTCAAGAA GCAGCTCACT 
GAGCCAGTAC TCCTTTATCA ACTCGTTCAA CTTCTTCTAA CCTATGAACC ACAGATTGTG 
CAGAGAGTTG CTCTACTACT GTATCTTGTA ATGCAGGATA ACCCATTTCT TCCACGACTT 
TATCTTTCTG GTGTCTTCTA TTTTATTCTT ATGTACAATG GATCAAATGT TCTTCCGATT 
GCTCGATTCC TTCACTATAC CCACATGAAA CAAGCATTCC GTTCTACACT TCCACAATTT 
GAGGGACAAC GGCAATCAGT TCTTGCCACT CTTCTTCCAG AAGCCGCCAC ATTCTATTTG 
GAACAATATG GACCTGAAAA GTATGCCGAA GTGTTCCTTG GAGAATTCGA TAATCCCGAA 
ATCATTTGGA ATACGGCGAT GCGTCGACAT TTGATTGAAC GAATCGCTGT TCATGTTGCA 
GATTTCTCGC ATCGATTAAC ATCAAATGTT CGAGCTTTAT ATCAATTCTG TCCAATTCCT 
TTGATCGATT ATCCAGAACT TGCTCAGGAA CTCTTCTGTC ATGTTTATTA TCTTCGACAC 
TTGTGTAATA AGCAAAGATT CCCTGATTGG GAAATTAGGG ATCCGATTCC GTTCCTTCGA 
TGTTGCCTTG CCACATGGTA TAATGAGCTA GAGAAGAAGC CGGCGACGAT GTCAGTCGAT 
CTTGCAAGAG AGATTTTATC AGTTGATTTG ACTAACGAAG AACACCGAAA ACCTGCATTC 
ATACGTCGTC AATATTACAA ACTCGCTGCT AAATACCATC CTGACAAGAA TCCAGAAGGA 
AGAGAAATGT TCGAACGAAT AAATGCAGCT TATGAACTAT TGAGCAGTGA AACTGCAAAT 
AATTCAGGAA TGCCGGATTC GCATCGAATT GTGCTTTGTT TACAGGCACA ATCCATCATT 
TATAGTAGAT ATAGTCAAGA ATTATCCGAA TACAAATACG CTGGTTACTC TCAACTCATT 
AAAACTATCG ATTTGGAAGC AAAAGACGAA GCATTGTTCA TAAAAGGAGG TGGAGACCTG 
TTGTCAGCTG CTATTGAACT TGCTAATTAT ACACTTATTA GTAGTGCTTT GAATGCTGAA 
CAATTGAGAA GAGATAATGG ATTAGAGGCA TTAGTAACTG CATTTGATCG ATGTGTTCCA 
ATGGTAACAA TGAGTTCATT ACCTGATGAT ATGGCTGTTC GAGTTTGTAT TCATGTTTGT 
GATTGTTTTG CCACTGCTGC AACATTTGAA GCGTGTAGGC AGCGACTGAT GGAGATGCCA 
TCCATATTTG GAGCCCTCAC TAGATTACTT CAATTTTCGA ACTTACCCCG TCTCTCCACT 
GCAGCAGCTC AATGTATTCG TGCAATGGCA GTTGACACAC TTCTTCAATT CCAACTTTTC 
CAAACTGGTG TTCTATGGCA ATTGGTACCA CATCTCTTTC ATTTCGATTA TACATTGGAT 
GAAGGAGGTG TTCAACATTC AGAGGATTCT AATAAACAAT CACTCGCTAA TTCATTAGCT 
CGTTCTTCGT GTGAAGCTCT TGCAGCTCTG GCTGGATTCC GTGAGAATAC ACCGGATAAT 
GATGGAGTTC AGGCAAGCCT TCGAGCTCTA CTTACTCCTT ACATCTGTCG ATGCATGAAG 
CTTGAAACAA ATGATATGGT TCTGAAAACG CTGAATAGTA ATATGGAAAA TCCATATATG 
ATCTGGGATA ATGGAACAAG AGCTGAAGTT CTCGAATTTG TGGAGAGACA TCGAACTTCT 
AATGAGCCTA CGAGTGAGCT GTACGGAGCC GAATTCGAGT TATCTGTGCA CAAAAATGAG 
CTTATCGTTG GAGACATATT CATCAGAATC TACAATGAAC AGCCAACATT TGCTCTTCAC 
GAACCCAAAA AAGTTGCAAT CGATCTTTTG GATTACATCA AAAAACACTC GGCAGAACTA 
ACCGGAGCTC CAAAACCTAA AGCAATTTCG GATGATTTGA TTGAAATTGA CTGGGGAAAT 
GGATCACAGG CTAATGGAAA CTCATTGGAC ACAGAAACAA AAGTTCTCAT GACAATGACT 
GCATTAGCTA ATTTAGTTTC AGCGAATCCA GGTGTTGAAA TTCTACTAAT TGGAAACTTC 
CCACTTCTTA TCACTTATCT ACGTTGTCGA AAACATCCGA AGCTTCAAAT TGCTGCACTT 
CAAGTCATTC TGTTGGCTGC AGCTAATAAG GAATGTGTAA CTGATTTGGC CACGTGTAAT 
GTCACTACAA CACTTTTCAC ACTTTTACGT GATCAGCCGA AGATGATAGC AAGAGTATTA 
GATGTTCTGA TTGCACTTTC ATCAAATGGA CAAATTGGAA AAGAAATGTT AGAACATGGA 
GGACTTATGT ATATTCTATC AATTCTTTGT TTGACAAATT CTGATCAAGG CCAAAGACTG 
CAGGCAGCCG AGCTTTTGGC CAAACTTCAA GCTGATAAGC TTACTGGGCC AAGATGGACA 
CGATTTATTA TCAAGTTTTT GCCAGAAATA TTTGCAGATT CATTGAGAGA TTCGCCCAAT 
ACAGCCCTTC AAATGTTCGA TTCATCAAGC GAAAATCCAG AGCTTATTTG GAATGAAGTA 
ACGCGTCAAA AAGTGAAAGG AATCATTGAT ACAGAAGTCG GAAAACTCTA TGAACAACAC 
ATCAAAGATC CAACTGTCAA ATGGAATACG ACTTCATTGG CTGACAAGAG TTGTGCCTAT 
GGTGATAGTA TTAATGGTGA ACTTGTTGTT GGAGGTGTTT TTGTACGATT ATACGTCGAA 
AATCCAGGAT TCAATGTCAG ACATCCAAAA TTATTCTCAA CAGAGCTCAT CGAAAAAGTG 
CTTGAACTGA TGAGTCGTCC AACTCCAGAA CTCGATTTGG TCACAAAAGC CTTTGTTGAA 
CTGGTCCGTC ATCATCCGAA TACTGCTGAT CAATTGCCAT CTCAAGGATA TCTACCACAG 
TTCTGCACTG CAATGTGTCT TCAAAACACA TCTGCGTCTC GTTCAGCAAT TCTAATTCTT 
CAAGAATTGA GCGAAAATCA ATTCTGTTGT GATGCTCTTT CTCAGCTGCC ATGTATTGAT 
GGAATAATGA AATCAATGAA GAATCAACCA AGTTTGATGA GAGAATCGGC GCACGCTTTA 
AAATGTCTCA TGAAGAGAAA TACAGGAGAA TTGGCTCAAC AAATGCTCTC ATGTGGAATG 
GTTCCATATT TGTTACAAGT TTTGGATAGT TCAATGAATG GAGTATCAAA CGGAGCAGCT 
GCTCGTGCCG AGATTGTTGA CGCATTAAAA AGCGCAATTT TAGATCTGAA AGTTGGTCAG 
AAGATTGCTG AGATTTTAGA CAAGAGTCCC GTCTGGGCAC AGTTTAAAGA TCAACGACAT 
GATTTATTCT TGCCAGAAGC GAGAACTCAA GCTATTACAG GAGGTCCAAC TGGTGTTGCT 
GGATATCTAA CAGAAGGAAT GTTCAATCCA CCACCAATGA GCAATCAGCC ACCACCAATG 
CATCAAACAT CTGGAGATTT CCAACCACCA CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021395.1
CDS	1..6816
Translation	MAQFCSENRDIACYLVTKHSWKGKYKRVFSIGTLAITTYNPNTLEITNQWLYEDFLSVKPLRNGGSNDSKQDEYKIHVRNRGKNNDMRFSSDYTTDILTHCLQFSTKFADKNFEPLTVTAFKQSWSDRRIPVTLRANASCLEQIDNRGVVVQSYPYKNIRSIGKVSDCPGGFVVDVGEHRRRHMFASSNNEELVKEIRRLASDNIGIIVPIAKEQLTLEDFMRTRLGLCSRDEELTSYAEFKISKITRRNEMPVRRLLCLSETCIIERDLATYAVICATPLKHIVCLVRSEKDPQQFIVEYENGDGRAYVAAERDLILASLLDGIRASGNNEVFVCGHRFERNLRVIPFSTNLDEDSESQCMKHIIAPPPGLRRCDLIRRFNANVPYSGLRFSKSHEGFFSENKGKVIVNAIEAVLMENYTKDDKEYKHKTEAQLQCLRRLFASKSGFQAFTEVNGVREKLGSLVVRVLSWKSESIDHSTVEALCALMYPMHDQYELRIEQLNKQSLMSSPKFVENLLDLIVLHVDRSTGWLVIASMLNFLTFSVCSPYSETTAGDTFDHILKLVSLRGRSFFRLFQCPSMTIVKGAGMVMRAIIEEADVETSKSMQMLALSEGAFLTHLFMSLLSTGKDLRVMTNKQLSGHLISLWIADNQQANDLLIRCLPRGLLNYMESDEKVPVNEKDLLIVRNNFDAASNETKQNAMKEKFDQLRVTAEAGLERFVQHWDLEQKLNFLPKRVIDEKQQQRQQPVVLRKRRNRVRPNVNWKLFAYQFSKDHSQADLIWNEKTREEFRHAMDTETRALISEKEQAPTGLPIAWNHTEFQIRYPSLLEEIKIGDYYLRLLLIEADENATPIHNPLEFFNNVYHRFLLSTKVDMKCLCLRAMAITYSRHHMTIGAFQDSKYFVEMLQKCINPLERDHLVLLLSKLALNKDNVRELIISNILPLLVDLCVLAHLHVQRAKVQNQTNVIEASAEQMAEGGSEEWYYHDKDAKQVGPLSFEKMKSLYTEKTIFEKSQIWAAGMDKWMSLAAVPQFRWTVCQQKDQPNEINTGKGPFQATVMNFTDLSVLCLDTILQMCEFFPSRDSHDCVVRPMPSVKKQLTEPVLLYQLVQLLLTYEPQIVQRVALLLYLVMQDNPFLPRLYLSGVFYFILMYNGSNVLPIARFLHYTHMKQAFRSTLPQFEGQRQSVLATLLPEAATFYLEQYGPEKYAEVFLGEFDNPEIIWNTAMRRHLIERIAVHVADFSHRLTSNVRALYQFCPIPLIDYPELAQELFCHVYYLRHLCNKQRFPDWEIRDPIPFLRCCLATWYNELEKKPATMSVDLAREILSVDLTNEEHRKPAFIRRQYYKLAAKYHPDKNPEGREMFERINAAYELLSSETANNSGMPDSHRIVLCLQAQSIIYSRYSQELSEYKYAGYSQLIKTIDLEAKDEALFIKGGGDLLSAAIELANYTLISSALNAEQLRRDNGLEALVTAFDRCVPMVTMSSLPDDMAVRVCIHVCDCFATAATFEACRQRLMEMPSIFGALTRLLQFSNLPRLSTAAAQCIRAMAVDTLLQFQLFQTGVLWQLVPHLFHFDYTLDEGGVQHSEDSNKQSLANSLARSSCEALAALAGFRENTPDNDGVQASLRALLTPYICRCMKLETNDMVLKTLNSNMENPYMIWDNGTRAEVLEFVERHRTSNEPTSELYGAEFELSVHKNELIVGDIFIRIYNEQPTFALHEPKKVAIDLLDYIKKHSAELTGAPKPKAISDDLIEIDWGNGSQANGNSLDTETKVLMTMTALANLVSANPGVEILLIGNFPLLITYLRCRKHPKLQIAALQVILLAAANKECVTDLATCNVTTTLFTLLRDQPKMIARVLDVLIALSSNGQIGKEMLEHGGLMYILSILCLTNSDQGQRLQAAELLAKLQADKLTGPRWTRFIIKFLPEIFADSLRDSPNTALQMFDSSSENPELIWNEVTRQKVKGIIDTEVGKLYEQHIKDPTVKWNTTSLADKSCAYGDSINGELVVGGVFVRLYVENPGFNVRHPKLFSTELIEKVLELMSRPTPELDLVTKAFVELVRHHPNTADQLPSQGYLPQFCTAMCLQNTSASRSAILILQELSENQFCCDALSQLPCIDGIMKSMKNQPSLMRESAHALKCLMKRNTGELAQQMLSCGMVPYLLQVLDSSMNGVSNGAAARAEIVDALKSAILDLKVGQKIAEILDKSPVWAQFKDQRHDLFLPEARTQAITGGPTGVAGYLTEGMFNPPPMSNQPPPMHQTSGDFQPPL

Target ORF information:

RefSeq Version	NM_001026224.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Receptor Mediated Endocytosis (rme-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026224.3

ORF Insert Sequence:

ATGGCTCAAT TTTGCTCGGA AAACCGAGAC ATTGCATGCT ATTTGGTCAC AAAACACTCA 
TGGAAGGGAA AGTACAAACG GGTGTTTTCT ATCGGGACAC TGGCCATCAC CACTTACAAT 
CCTAATACCT TGGAGATTAC CAATCAATGG CTCTACGAGG ACTTCTTATC AGTGAAACCA 
CTTCGAAATG GAGGTTCAAA TGATTCAAAA CAGGATGAAT ACAAGATTCA TGTTAGGAAT 
CGTGGAAAAA ACAATGATAT GCGTTTTTCA TCTGATTATA CCACCGATAT ACTTACACAT 
TGTCTGCAAT TCAGCACCAA GTTCGCTGAT AAGAACTTTG AACCTTTGAC GGTAACTGCA 
TTCAAACAAA GTTGGAGTGA TCGTCGAATT CCCGTAACTC TACGAGCAAA TGCTTCTTGT 
TTGGAGCAAA TTGATAATCG TGGAGTCGTC GTTCAAAGTT ATCCGTACAA AAATATTCGA 
AGTATTGGAA AAGTGTCCGA TTGTCCCGGT GGTTTTGTGG TTGATGTGGG AGAACATAGA 
CGGAGGCACA TGTTCGCTTC TTCAAACAAC GAAGAACTAG TGAAAGAGAT TCGTCGTTTA 
GCATCTGATA ATATCGGAAT TATCGTTCCA ATTGCTAAAG AGCAGCTAAC ACTCGAAGAT 
TTTATGAGAA CTCGACTCGG ATTGTGCAGT CGAGATGAAG AGCTCACTTC ATATGCTGAA 
TTCAAAATTT CCAAAATTAC TCGAAGAAAT GAGATGCCTG TTCGACGGCT TCTATGTCTC 
AGTGAGACGT GTATTATTGA AAGAGACTTG GCTACTTATG CAGTGATTTG TGCAACTCCA 
CTGAAACATA TCGTTTGCCT TGTTCGAAGT GAAAAAGATC CCCAACAATT CATTGTTGAA 
TACGAAAATG GAGATGGGCG AGCATATGTT GCTGCTGAAA GAGATTTGAT CTTAGCCTCA 
CTCTTAGATG GAATTCGTGC TTCTGGAAAT AATGAAGTGT TCGTTTGTGG ACACAGATTT 
GAAAGGAATT TGAGAGTGAT CCCATTCTCA ACGAATTTGG ATGAAGATAG TGAATCTCAA 
TGTATGAAGC ATATTATTGC ACCTCCACCT GGACTTCGTC GTTGTGATTT GATCCGTCGC 
TTCAACGCAA ATGTTCCATA TTCTGGTCTT CGATTTTCAA AGTCGCACGA AGGATTCTTC 
TCAGAAAACA AAGGAAAAGT TATTGTAAAT GCAATTGAGG CAGTTCTAAT GGAGAATTAT 
ACAAAAGACG ATAAGGAGTA CAAGCACAAA ACTGAAGCTC AGTTACAGTG TTTACGAAGA 
CTTTTTGCGT CGAAAAGTGG ATTCCAGGCA TTTACAGAAG TTAATGGTGT TCGTGAGAAG 
CTTGGGAGTC TTGTGGTTCG AGTTCTATCT TGGAAATCTG AATCAATTGA TCATTCGACG 
GTTGAAGCTC TTTGTGCATT GATGTATCCA ATGCATGATC AATATGAACT TAGAATAGAG 
CAACTGAACA AGCAGAGTCT GATGAGCAGC CCAAAATTTG TGGAGAACTT ACTCGATTTG 
ATTGTTCTTC ACGTGGATCG TTCAACTGGA TGGCTTGTGA TTGCATCAAT GCTCAATTTC 
CTCACATTTT CCGTTTGCTC TCCGTACTCT GAAACCACCG CTGGAGATAC TTTCGATCAT 
ATTCTGAAAC TCGTATCTCT CCGAGGGCGC AGCTTTTTCC GTTTATTCCA ATGTCCCAGT 
ATGACAATTG TTAAAGGTGC TGGAATGGTT ATGAGAGCAA TTATCGAAGA AGCTGATGTT 
GAAACTTCAA AATCAATGCA AATGTTAGCA CTATCAGAAG GAGCATTCTT GACACATTTG 
TTCATGTCAT TATTGTCAAC TGGAAAAGAT TTACGAGTAA TGACAAATAA ACAATTGTCT 
GGACATTTGA TAAGTCTTTG GATAGCTGAT AATCAACAGG CTAATGATTT GTTAATTAGG 
TGCCTGCCCC GTGGTCTATT GAACTATATG GAGTCAGACG AAAAAGTTCC AGTAAACGAG 
AAAGATCTTC TCATTGTTCG AAATAACTTT GATGCAGCAT CAAATGAAAC TAAGCAGAAT 
GCGATGAAGG AGAAGTTTGA CCAGCTTCGA GTTACTGCTG AAGCTGGTTT GGAAAGATTT 
GTGCAGCACT GGGATCTCGA ACAAAAACTC AATTTTCTTC CAAAACGAGT TATTGATGAG 
AAACAACAAC AAAGACAGCA ACCGGTTGTC CTGAGAAAAC GAAGAAATCG AGTACGGCCA 
AATGTTAATT GGAAACTATT TGCATATCAA TTCTCGAAGG ATCACTCTCA GGCAGATTTA 
ATTTGGAATG AGAAGACGCG AGAAGAGTTC CGTCATGCAA TGGATACAGA AACACGAGCT 
CTTATCAGTG AAAAAGAACA AGCACCAACT GGATTGCCGA TTGCTTGGAA TCATACTGAG 
TTCCAAATCA GATATCCATC ACTTTTAGAA GAAATTAAAA TTGGAGATTA TTACCTTCGA 
TTGCTTCTGA TTGAGGCAGA CGAAAATGCG ACTCCGATTC ATAATCCGCT GGAGTTCTTC 
AACAACGTCT ATCATCGTTT CCTACTTTCC ACTAAAGTTG ACATGAAATG TCTGTGCCTT 
CGTGCAATGG CTATCACCTA TTCTCGACAT CATATGACAA TCGGAGCATT TCAGGATTCA 
AAGTATTTCG TAGAAATGCT TCAAAAATGC ATAAATCCGT TGGAAAGAGA TCATCTTGTG 
CTTCTGTTAT CGAAACTAGC ACTGAATAAG GATAATGTTC GAGAACTTAT AATATCCAAT 
ATTCTTCCAC TTTTGGTAGA TCTTTGTGTT CTTGCACATT TACACGTGCA GCGAGCAAAA 
GTTCAAAATC AGACAAATGT GATTGAGGCA AGTGCCGAAC AAATGGCTGA AGGAGGTTCT 
GAAGAATGGT ATTATCATGA TAAGGATGCT AAACAAGTCG GACCATTGTC ATTTGAGAAA 
ATGAAGTCTC TTTACACGGA GAAGACTATT TTTGAGAAAT CTCAAATTTG GGCAGCTGGA 
ATGGACAAGT GGATGAGTTT GGCTGCAGTT CCACAATTCA GATGGACTGT TTGTCAGCAA 
AAAGATCAGC CGAACGAGAT TAATACAGGA AAAGGACCAT TCCAGGCAAC TGTCATGAAC 
TTCACAGATC TCAGTGTTCT TTGTTTAGAT ACTATTCTTC AGATGTGCGA ATTTTTCCCA 
TCAAGGGATT CTCATGATTG TGTGGTCCGT CCGATGCCAT CTGTCAAGAA GCAGCTCACT 
GAGCCAGTAC TCCTTTATCA ACTCGTTCAA CTTCTTCTAA CCTATGAACC ACAGATTGTG 
CAGAGAGTTG CTCTACTACT GTATCTTGTA ATGCAGGATA ACCCATTTCT TCCACGACTT 
TATCTTTCTG GTGTCTTCTA TTTTATTCTT ATGTACAATG GATCAAATGT TCTTCCGATT 
GCTCGATTCC TTCACTATAC CCACATGAAA CAAGCATTCC GTTCTACACT TCCACAATTT 
GAGGGACAAC GGCAATCAGT TCTTGCCACT CTTCTTCCAG AAGCCGCCAC ATTCTATTTG 
GAACAATATG GACCTGAAAA GTATGCCGAA GTGTTCCTTG GAGAATTCGA TAATCCCGAA 
ATCATTTGGA ATACGGCGAT GCGTCGACAT TTGATTGAAC GAATCGCTGT TCATGTTGCA 
GATTTCTCGC ATCGATTAAC ATCAAATGTT CGAGCTTTAT ATCAATTCTG TCCAATTCCT 
TTGATCGATT ATCCAGAACT TGCTCAGGAA CTCTTCTGTC ATGTTTATTA TCTTCGACAC 
TTGTGTAATA AGCAAAGATT CCCTGATTGG GAAATTAGGG ATCCGATTCC GTTCCTTCGA 
TGTTGCCTTG CCACATGGTA TAATGAGCTA GAGAAGAAGC CGGCGACGAT GTCAGTCGAT 
CTTGCAAGAG AGATTTTATC AGTTGATTTG ACTAACGAAG AACACCGAAA ACCTGCATTC 
ATACGTCGTC AATATTACAA ACTCGCTGCT AAATACCATC CTGACAAGAA TCCAGAAGGA 
AGAGAAATGT TCGAACGAAT AAATGCAGCT TATGAACTAT TGAGCAGTGA AACTGCAAAT 
AATTCAGGAA TGCCGGATTC GCATCGAATT GTGCTTTGTT TACAGGCACA ATCCATCATT 
TATAGTAGAT ATAGTCAAGA ATTATCCGAA TACAAATACG CTGGTTACTC TCAACTCATT 
AAAACTATCG ATTTGGAAGC AAAAGACGAA GCATTGTTCA TAAAAGGAGG TGGAGACCTG 
TTGTCAGCTG CTATTGAACT TGCTAATTAT ACACTTATTA GTAGTGCTTT GAATGCTGAA 
CAATTGAGAA GAGATAATGG ATTAGAGGCA TTAGTAACTG CATTTGATCG ATGTGTTCCA 
ATGGTAACAA TGAGTTCATT ACCTGATGAT ATGGCTGTTC GAGTTTGTAT TCATGTTTGT 
GATTGTTTTG CCACTGCTGC AACATTTGAA GCGTGTAGGC AGCGACTGAT GGAGATGCCA 
TCCATATTTG GAGCCCTCAC TAGATTACTT CAATTTTCGA ACTTACCCCG TCTCTCCACT 
GCAGCAGCTC AATGTATTCG TGCAATGGCA GTTGACACAC TTCTTCAATT CCAACTTTTC 
CAAACTGGTG TTCTATGGCA ATTGGTACCA CATCTCTTTC ATTTCGATTA TACATTGGAT 
GAAGGAGGTG TTCAACATTC AGAGGATTCT AATAAACAAT CACTCGCTAA TTCATTAGCT 
CGTTCTTCGT GTGAAGCTCT TGCAGCTCTG GCTGGATTCC GTGAGAATAC ACCGGATAAT 
GATGGAGTTC AGGCAAGCCT TCGAGCTCTA CTTACTCCTT ACATCTGTCG ATGCATGAAG 
CTTGAAACAA ATGATATGGT TCTGAAAACG CTGAATAGTA ATATGGAAAA TCCATATATG 
ATCTGGGATA ATGGAACAAG AGCTGAAGTT CTCGAATTTG TGGAGAGACA TCGAACTTCT 
AATGAGCCTA CGAGTGAGCT GTACGGAGCC GAATTCGAGT TATCTGTGCA CAAAAATGAG 
CTTATCGTTG GAGACATATT CATCAGAATC TACAATGAAC AGCCAACATT TGCTCTTCAC 
GAACCCAAAA AAGTTGCAAT CGATCTTTTG GATTACATCA AAAAACACTC GGCAGAACTA 
ACCGGAGCTC CAAAACCTAA AGCAATTTCG GATGATTTGA TTGAAATTGA CTGGGGAAAT 
GGATCACAGG CTAATGGAAA CTCATTGGAC ACAGAAACAA AAGTTCTCAT GACAATGACT 
GCATTAGCTA ATTTAGTTTC AGCGAATCCA GGTGTTGAAA TTCTACTAAT TGGAAACTTC 
CCACTTCTTA TCACTTATCT ACGTTGTCGA AAACATCCGA AGCTTCAAAT TGCTGCACTT 
CAAGTCATTC TGTTGGCTGC AGCTAATAAG GAATGTGTAA CTGATTTGGC CACGTGTAAT 
GTCACTACAA CACTTTTCAC ACTTTTACGT GATCAGCCGA AGATGATAGC AAGAGTATTA 
GATGTTCTGA TTGCACTTTC ATCAAATGGA CAAATTGGAA AAGAAATGTT AGAACATGGA 
GGACTTATGT ATATTCTATC AATTCTTTGT TTGACAAATT CTGATCAAGG CCAAAGACTG 
CAGGCAGCCG AGCTTTTGGC CAAACTTCAA GCTGATAAGC TTACTGGGCC AAGATGGACA 
CGATTTATTA TCAAGTTTTT GCCAGAAATA TTTGCAGATT CATTGAGAGA TTCGCCCAAT 
ACAGCCCTTC AAATGTTCGA TTCATCAAGC GAAAATCCAG AGCTTATTTG GAATGAAGTA 
ACGCGTCAAA AAGTGAAAGG AATCATTGAT ACAGAAGTCG GAAAACTCTA TGAACAACAC 
ATCAAAGATC CAACTGTCAA ATGGAATACG ACTTCATTGG CTGACAAGAG TTGTGCCTAT 
GGTGATAGTA TTAATGGTGA ACTTGTTGTT GGAGGTGTTT TTGTACGATT ATACGTCGAA 
AATCCAGGAT TCAATGTCAG ACATCCAAAA TTATTCTCAA CAGAGCTCAT CGAAAAAGTG 
CTTGAACTGA TGAGTCGTCC AACTCCAGAA CTCGATTTGG TCACAAAAGC CTTTGTTGAA 
CTGGTCCGTC ATCATCCGAA TACTGCTGAT CAATTGCCAT CTCAAGGATA TCTACCACAG 
TTCTGCACTG CAATGTGTCT TCAAAACACA TCTGCGTCTC GTTCAGCAAT TCTAATTCTT 
CAAGAATTGA GCGAAAATCA ATTCTGTTGT GATGCTCTTT CTCAGCTGCC ATGTATTGAT 
GGAATAATGA AATCAATGAA GAATCAACCA AGTTTGATGA GAGAATCGGC GCACGCTTTA 
AAATGTCTCA TGAAGAGAAA TACAGGAGAA TTGGCTCAAC AAATGCTCTC ATGTGGAATG 
GTTCCATATT TGTTACAAGT TTTGGATAGT TCAATGAATG GAGTATCAAA CGGAGCAGCT 
GCTCGTGCCG AGATTGTTGA CGCATTAAAA AGCGCAATTT TAGATCTGAA AGTTGGTCAG 
AAGATTGCTG AGATTTTAGA CAAGAGTCCC GTCTGGGCAC AGTTTAAAGA TCAACGACAT 
GATTTATTCT TGCCAGAAGC GAGAACTCAA GCTATTACAG GAGGTCCAAC TGGTGTTGCT 
GGATATCTAA CAGAAGGAAT GTTCAATCCA CCACCAATGA GCAATCAGCC ACCACCAATG 
CATCAAACAT CTGGAGATTT CCAACCACCA CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rme-8 ( NM_001026224.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021395.1 Caenorhabditis elegans J domain-containing protein (rme-8), mRNA.

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CloneID	OCa335310
Clone ID Related Accession (Same CDS sequence)	NM_001026224.3 , NM_001026224.4
Accession Version	NM_001026224.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	J domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001026224.3.

ATGGCTCAAT TTTGCTCGGA AAACCGAGAC ATTGCATGCT ATTTGGTCAC AAAACACTCA 
TGGAAGGGAA AGTACAAACG GGTGTTTTCT ATCGGGACAC TGGCCATCAC CACTTACAAT 
CCTAATACCT TGGAGATTAC CAATCAATGG CTCTACGAGG ACTTCTTATC AGTGAAACCA 
CTTCGAAATG GAGGTTCAAA TGATTCAAAA CAGGATGAAT ACAAGATTCA TGTTAGGAAT 
CGTGGAAAAA ACAATGATAT GCGTTTTTCA TCTGATTATA CCACCGATAT ACTTACACAT 
TGTCTGCAAT TCAGCACCAA GTTCGCTGAT AAGAACTTTG AACCTTTGAC GGTAACTGCA 
TTCAAACAAA GTTGGAGTGA TCGTCGAATT CCCGTAACTC TACGAGCAAA TGCTTCTTGT 
TTGGAGCAAA TTGATAATCG TGGAGTCGTC GTTCAAAGTT ATCCGTACAA AAATATTCGA 
AGTATTGGAA AAGTGTCCGA TTGTCCCGGT GGTTTTGTGG TTGATGTGGG AGAACATAGA 
CGGAGGCACA TGTTCGCTTC TTCAAACAAC GAAGAACTAG TGAAAGAGAT TCGTCGTTTA 
GCATCTGATA ATATCGGAAT TATCGTTCCA ATTGCTAAAG AGCAGCTAAC ACTCGAAGAT 
TTTATGAGAA CTCGACTCGG ATTGTGCAGT CGAGATGAAG AGCTCACTTC ATATGCTGAA 
TTCAAAATTT CCAAAATTAC TCGAAGAAAT GAGATGCCTG TTCGACGGCT TCTATGTCTC 
AGTGAGACGT GTATTATTGA AAGAGACTTG GCTACTTATG CAGTGATTTG TGCAACTCCA 
CTGAAACATA TCGTTTGCCT TGTTCGAAGT GAAAAAGATC CCCAACAATT CATTGTTGAA 
TACGAAAATG GAGATGGGCG AGCATATGTT GCTGCTGAAA GAGATTTGAT CTTAGCCTCA 
CTCTTAGATG GAATTCGTGC TTCTGGAAAT AATGAAGTGT TCGTTTGTGG ACACAGATTT 
GAAAGGAATT TGAGAGTGAT CCCATTCTCA ACGAATTTGG ATGAAGATAG TGAATCTCAA 
TGTATGAAGC ATATTATTGC ACCTCCACCT GGACTTCGTC GTTGTGATTT GATCCGTCGC 
TTCAACGCAA ATGTTCCATA TTCTGGTCTT CGATTTTCAA AGTCGCACGA AGGATTCTTC 
TCAGAAAACA AAGGAAAAGT TATTGTAAAT GCAATTGAGG CAGTTCTAAT GGAGAATTAT 
ACAAAAGACG ATAAGGAGTA CAAGCACAAA ACTGAAGCTC AGTTACAGTG TTTACGAAGA 
CTTTTTGCGT CGAAAAGTGG ATTCCAGGCA TTTACAGAAG TTAATGGTGT TCGTGAGAAG 
CTTGGGAGTC TTGTGGTTCG AGTTCTATCT TGGAAATCTG AATCAATTGA TCATTCGACG 
GTTGAAGCTC TTTGTGCATT GATGTATCCA ATGCATGATC AATATGAACT TAGAATAGAG 
CAACTGAACA AGCAGAGTCT GATGAGCAGC CCAAAATTTG TGGAGAACTT ACTCGATTTG 
ATTGTTCTTC ACGTGGATCG TTCAACTGGA TGGCTTGTGA TTGCATCAAT GCTCAATTTC 
CTCACATTTT CCGTTTGCTC TCCGTACTCT GAAACCACCG CTGGAGATAC TTTCGATCAT 
ATTCTGAAAC TCGTATCTCT CCGAGGGCGC AGCTTTTTCC GTTTATTCCA ATGTCCCAGT 
ATGACAATTG TTAAAGGTGC TGGAATGGTT ATGAGAGCAA TTATCGAAGA AGCTGATGTT 
GAAACTTCAA AATCAATGCA AATGTTAGCA CTATCAGAAG GAGCATTCTT GACACATTTG 
TTCATGTCAT TATTGTCAAC TGGAAAAGAT TTACGAGTAA TGACAAATAA ACAATTGTCT 
GGACATTTGA TAAGTCTTTG GATAGCTGAT AATCAACAGG CTAATGATTT GTTAATTAGG 
TGCCTGCCCC GTGGTCTATT GAACTATATG GAGTCAGACG AAAAAGTTCC AGTAAACGAG 
AAAGATCTTC TCATTGTTCG AAATAACTTT GATGCAGCAT CAAATGAAAC TAAGCAGAAT 
GCGATGAAGG AGAAGTTTGA CCAGCTTCGA GTTACTGCTG AAGCTGGTTT GGAAAGATTT 
GTGCAGCACT GGGATCTCGA ACAAAAACTC AATTTTCTTC CAAAACGAGT TATTGATGAG 
AAACAACAAC AAAGACAGCA ACCGGTTGTC CTGAGAAAAC GAAGAAATCG AGTACGGCCA 
AATGTTAATT GGAAACTATT TGCATATCAA TTCTCGAAGG ATCACTCTCA GGCAGATTTA 
ATTTGGAATG AGAAGACGCG AGAAGAGTTC CGTCATGCAA TGGATACAGA AACACGAGCT 
CTTATCAGTG AAAAAGAACA AGCACCAACT GGATTGCCGA TTGCTTGGAA TCATACTGAG 
TTCCAAATCA GATATCCATC ACTTTTAGAA GAAATTAAAA TTGGAGATTA TTACCTTCGA 
TTGCTTCTGA TTGAGGCAGA CGAAAATGCG ACTCCGATTC ATAATCCGCT GGAGTTCTTC 
AACAACGTCT ATCATCGTTT CCTACTTTCC ACTAAAGTTG ACATGAAATG TCTGTGCCTT 
CGTGCAATGG CTATCACCTA TTCTCGACAT CATATGACAA TCGGAGCATT TCAGGATTCA 
AAGTATTTCG TAGAAATGCT TCAAAAATGC ATAAATCCGT TGGAAAGAGA TCATCTTGTG 
CTTCTGTTAT CGAAACTAGC ACTGAATAAG GATAATGTTC GAGAACTTAT AATATCCAAT 
ATTCTTCCAC TTTTGGTAGA TCTTTGTGTT CTTGCACATT TACACGTGCA GCGAGCAAAA 
GTTCAAAATC AGACAAATGT GATTGAGGCA AGTGCCGAAC AAATGGCTGA AGGAGGTTCT 
GAAGAATGGT ATTATCATGA TAAGGATGCT AAACAAGTCG GACCATTGTC ATTTGAGAAA 
ATGAAGTCTC TTTACACGGA GAAGACTATT TTTGAGAAAT CTCAAATTTG GGCAGCTGGA 
ATGGACAAGT GGATGAGTTT GGCTGCAGTT CCACAATTCA GATGGACTGT TTGTCAGCAA 
AAAGATCAGC CGAACGAGAT TAATACAGGA AAAGGACCAT TCCAGGCAAC TGTCATGAAC 
TTCACAGATC TCAGTGTTCT TTGTTTAGAT ACTATTCTTC AGATGTGCGA ATTTTTCCCA 
TCAAGGGATT CTCATGATTG TGTGGTCCGT CCGATGCCAT CTGTCAAGAA GCAGCTCACT 
GAGCCAGTAC TCCTTTATCA ACTCGTTCAA CTTCTTCTAA CCTATGAACC ACAGATTGTG 
CAGAGAGTTG CTCTACTACT GTATCTTGTA ATGCAGGATA ACCCATTTCT TCCACGACTT 
TATCTTTCTG GTGTCTTCTA TTTTATTCTT ATGTACAATG GATCAAATGT TCTTCCGATT 
GCTCGATTCC TTCACTATAC CCACATGAAA CAAGCATTCC GTTCTACACT TCCACAATTT 
GAGGGACAAC GGCAATCAGT TCTTGCCACT CTTCTTCCAG AAGCCGCCAC ATTCTATTTG 
GAACAATATG GACCTGAAAA GTATGCCGAA GTGTTCCTTG GAGAATTCGA TAATCCCGAA 
ATCATTTGGA ATACGGCGAT GCGTCGACAT TTGATTGAAC GAATCGCTGT TCATGTTGCA 
GATTTCTCGC ATCGATTAAC ATCAAATGTT CGAGCTTTAT ATCAATTCTG TCCAATTCCT 
TTGATCGATT ATCCAGAACT TGCTCAGGAA CTCTTCTGTC ATGTTTATTA TCTTCGACAC 
TTGTGTAATA AGCAAAGATT CCCTGATTGG GAAATTAGGG ATCCGATTCC GTTCCTTCGA 
TGTTGCCTTG CCACATGGTA TAATGAGCTA GAGAAGAAGC CGGCGACGAT GTCAGTCGAT 
CTTGCAAGAG AGATTTTATC AGTTGATTTG ACTAACGAAG AACACCGAAA ACCTGCATTC 
ATACGTCGTC AATATTACAA ACTCGCTGCT AAATACCATC CTGACAAGAA TCCAGAAGGA 
AGAGAAATGT TCGAACGAAT AAATGCAGCT TATGAACTAT TGAGCAGTGA AACTGCAAAT 
AATTCAGGAA TGCCGGATTC GCATCGAATT GTGCTTTGTT TACAGGCACA ATCCATCATT 
TATAGTAGAT ATAGTCAAGA ATTATCCGAA TACAAATACG CTGGTTACTC TCAACTCATT 
AAAACTATCG ATTTGGAAGC AAAAGACGAA GCATTGTTCA TAAAAGGAGG TGGAGACCTG 
TTGTCAGCTG CTATTGAACT TGCTAATTAT ACACTTATTA GTAGTGCTTT GAATGCTGAA 
CAATTGAGAA GAGATAATGG ATTAGAGGCA TTAGTAACTG CATTTGATCG ATGTGTTCCA 
ATGGTAACAA TGAGTTCATT ACCTGATGAT ATGGCTGTTC GAGTTTGTAT TCATGTTTGT 
GATTGTTTTG CCACTGCTGC AACATTTGAA GCGTGTAGGC AGCGACTGAT GGAGATGCCA 
TCCATATTTG GAGCCCTCAC TAGATTACTT CAATTTTCGA ACTTACCCCG TCTCTCCACT 
GCAGCAGCTC AATGTATTCG TGCAATGGCA GTTGACACAC TTCTTCAATT CCAACTTTTC 
CAAACTGGTG TTCTATGGCA ATTGGTACCA CATCTCTTTC ATTTCGATTA TACATTGGAT 
GAAGGAGGTG TTCAACATTC AGAGGATTCT AATAAACAAT CACTCGCTAA TTCATTAGCT 
CGTTCTTCGT GTGAAGCTCT TGCAGCTCTG GCTGGATTCC GTGAGAATAC ACCGGATAAT 
GATGGAGTTC AGGCAAGCCT TCGAGCTCTA CTTACTCCTT ACATCTGTCG ATGCATGAAG 
CTTGAAACAA ATGATATGGT TCTGAAAACG CTGAATAGTA ATATGGAAAA TCCATATATG 
ATCTGGGATA ATGGAACAAG AGCTGAAGTT CTCGAATTTG TGGAGAGACA TCGAACTTCT 
AATGAGCCTA CGAGTGAGCT GTACGGAGCC GAATTCGAGT TATCTGTGCA CAAAAATGAG 
CTTATCGTTG GAGACATATT CATCAGAATC TACAATGAAC AGCCAACATT TGCTCTTCAC 
GAACCCAAAA AAGTTGCAAT CGATCTTTTG GATTACATCA AAAAACACTC GGCAGAACTA 
ACCGGAGCTC CAAAACCTAA AGCAATTTCG GATGATTTGA TTGAAATTGA CTGGGGAAAT 
GGATCACAGG CTAATGGAAA CTCATTGGAC ACAGAAACAA AAGTTCTCAT GACAATGACT 
GCATTAGCTA ATTTAGTTTC AGCGAATCCA GGTGTTGAAA TTCTACTAAT TGGAAACTTC 
CCACTTCTTA TCACTTATCT ACGTTGTCGA AAACATCCGA AGCTTCAAAT TGCTGCACTT 
CAAGTCATTC TGTTGGCTGC AGCTAATAAG GAATGTGTAA CTGATTTGGC CACGTGTAAT 
GTCACTACAA CACTTTTCAC ACTTTTACGT GATCAGCCGA AGATGATAGC AAGAGTATTA 
GATGTTCTGA TTGCACTTTC ATCAAATGGA CAAATTGGAA AAGAAATGTT AGAACATGGA 
GGACTTATGT ATATTCTATC AATTCTTTGT TTGACAAATT CTGATCAAGG CCAAAGACTG 
CAGGCAGCCG AGCTTTTGGC CAAACTTCAA GCTGATAAGC TTACTGGGCC AAGATGGACA 
CGATTTATTA TCAAGTTTTT GCCAGAAATA TTTGCAGATT CATTGAGAGA TTCGCCCAAT 
ACAGCCCTTC AAATGTTCGA TTCATCAAGC GAAAATCCAG AGCTTATTTG GAATGAAGTA 
ACGCGTCAAA AAGTGAAAGG AATCATTGAT ACAGAAGTCG GAAAACTCTA TGAACAACAC 
ATCAAAGATC CAACTGTCAA ATGGAATACG ACTTCATTGG CTGACAAGAG TTGTGCCTAT 
GGTGATAGTA TTAATGGTGA ACTTGTTGTT GGAGGTGTTT TTGTACGATT ATACGTCGAA 
AATCCAGGAT TCAATGTCAG ACATCCAAAA TTATTCTCAA CAGAGCTCAT CGAAAAAGTG 
CTTGAACTGA TGAGTCGTCC AACTCCAGAA CTCGATTTGG TCACAAAAGC CTTTGTTGAA 
CTGGTCCGTC ATCATCCGAA TACTGCTGAT CAATTGCCAT CTCAAGGATA TCTACCACAG 
TTCTGCACTG CAATGTGTCT TCAAAACACA TCTGCGTCTC GTTCAGCAAT TCTAATTCTT 
CAAGAATTGA GCGAAAATCA ATTCTGTTGT GATGCTCTTT CTCAGCTGCC ATGTATTGAT 
GGAATAATGA AATCAATGAA GAATCAACCA AGTTTGATGA GAGAATCGGC GCACGCTTTA 
AAATGTCTCA TGAAGAGAAA TACAGGAGAA TTGGCTCAAC AAATGCTCTC ATGTGGAATG 
GTTCCATATT TGTTACAAGT TTTGGATAGT TCAATGAATG GAGTATCAAA CGGAGCAGCT 
GCTCGTGCCG AGATTGTTGA CGCATTAAAA AGCGCAATTT TAGATCTGAA AGTTGGTCAG 
AAGATTGCTG AGATTTTAGA CAAGAGTCCC GTCTGGGCAC AGTTTAAAGA TCAACGACAT 
GATTTATTCT TGCCAGAAGC GAGAACTCAA GCTATTACAG GAGGTCCAAC TGGTGTTGCT 
GGATATCTAA CAGAAGGAAT GTTCAATCCA CCACCAATGA GCAATCAGCC ACCACCAATG 
CATCAAACAT CTGGAGATTT CCAACCACCA CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021395.1
CDS	48..6863
Translation	MAQFCSENRDIACYLVTKHSWKGKYKRVFSIGTLAITTYNPNTLEITNQWLYEDFLSVKPLRNGGSNDSKQDEYKIHVRNRGKNNDMRFSSDYTTDILTHCLQFSTKFADKNFEPLTVTAFKQSWSDRRIPVTLRANASCLEQIDNRGVVVQSYPYKNIRSIGKVSDCPGGFVVDVGEHRRRHMFASSNNEELVKEIRRLASDNIGIIVPIAKEQLTLEDFMRTRLGLCSRDEELTSYAEFKISKITRRNEMPVRRLLCLSETCIIERDLATYAVICATPLKHIVCLVRSEKDPQQFIVEYENGDGRAYVAAERDLILASLLDGIRASGNNEVFVCGHRFERNLRVIPFSTNLDEDSESQCMKHIIAPPPGLRRCDLIRRFNANVPYSGLRFSKSHEGFFSENKGKVIVNAIEAVLMENYTKDDKEYKHKTEAQLQCLRRLFASKSGFQAFTEVNGVREKLGSLVVRVLSWKSESIDHSTVEALCALMYPMHDQYELRIEQLNKQSLMSSPKFVENLLDLIVLHVDRSTGWLVIASMLNFLTFSVCSPYSETTAGDTFDHILKLVSLRGRSFFRLFQCPSMTIVKGAGMVMRAIIEEADVETSKSMQMLALSEGAFLTHLFMSLLSTGKDLRVMTNKQLSGHLISLWIADNQQANDLLIRCLPRGLLNYMESDEKVPVNEKDLLIVRNNFDAASNETKQNAMKEKFDQLRVTAEAGLERFVQHWDLEQKLNFLPKRVIDEKQQQRQQPVVLRKRRNRVRPNVNWKLFAYQFSKDHSQADLIWNEKTREEFRHAMDTETRALISEKEQAPTGLPIAWNHTEFQIRYPSLLEEIKIGDYYLRLLLIEADENATPIHNPLEFFNNVYHRFLLSTKVDMKCLCLRAMAITYSRHHMTIGAFQDSKYFVEMLQKCINPLERDHLVLLLSKLALNKDNVRELIISNILPLLVDLCVLAHLHVQRAKVQNQTNVIEASAEQMAEGGSEEWYYHDKDAKQVGPLSFEKMKSLYTEKTIFEKSQIWAAGMDKWMSLAAVPQFRWTVCQQKDQPNEINTGKGPFQATVMNFTDLSVLCLDTILQMCEFFPSRDSHDCVVRPMPSVKKQLTEPVLLYQLVQLLLTYEPQIVQRVALLLYLVMQDNPFLPRLYLSGVFYFILMYNGSNVLPIARFLHYTHMKQAFRSTLPQFEGQRQSVLATLLPEAATFYLEQYGPEKYAEVFLGEFDNPEIIWNTAMRRHLIERIAVHVADFSHRLTSNVRALYQFCPIPLIDYPELAQELFCHVYYLRHLCNKQRFPDWEIRDPIPFLRCCLATWYNELEKKPATMSVDLAREILSVDLTNEEHRKPAFIRRQYYKLAAKYHPDKNPEGREMFERINAAYELLSSETANNSGMPDSHRIVLCLQAQSIIYSRYSQELSEYKYAGYSQLIKTIDLEAKDEALFIKGGGDLLSAAIELANYTLISSALNAEQLRRDNGLEALVTAFDRCVPMVTMSSLPDDMAVRVCIHVCDCFATAATFEACRQRLMEMPSIFGALTRLLQFSNLPRLSTAAAQCIRAMAVDTLLQFQLFQTGVLWQLVPHLFHFDYTLDEGGVQHSEDSNKQSLANSLARSSCEALAALAGFRENTPDNDGVQASLRALLTPYICRCMKLETNDMVLKTLNSNMENPYMIWDNGTRAEVLEFVERHRTSNEPTSELYGAEFELSVHKNELIVGDIFIRIYNEQPTFALHEPKKVAIDLLDYIKKHSAELTGAPKPKAISDDLIEIDWGNGSQANGNSLDTETKVLMTMTALANLVSANPGVEILLIGNFPLLITYLRCRKHPKLQIAALQVILLAAANKECVTDLATCNVTTTLFTLLRDQPKMIARVLDVLIALSSNGQIGKEMLEHGGLMYILSILCLTNSDQGQRLQAAELLAKLQADKLTGPRWTRFIIKFLPEIFADSLRDSPNTALQMFDSSSENPELIWNEVTRQKVKGIIDTEVGKLYEQHIKDPTVKWNTTSLADKSCAYGDSINGELVVGGVFVRLYVENPGFNVRHPKLFSTELIEKVLELMSRPTPELDLVTKAFVELVRHHPNTADQLPSQGYLPQFCTAMCLQNTSASRSAILILQELSENQFCCDALSQLPCIDGIMKSMKNQPSLMRESAHALKCLMKRNTGELAQQMLSCGMVPYLLQVLDSSMNGVSNGAAARAEIVDALKSAILDLKVGQKIAEILDKSPVWAQFKDQRHDLFLPEARTQAITGGPTGVAGYLTEGMFNPPPMSNQPPPMHQTSGDFQPPL

Target ORF information:

RefSeq Version	NM_001026224.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans J domain-containing protein (rme-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026224.4

ORF Insert Sequence:

ATGGCTCAAT TTTGCTCGGA AAACCGAGAC ATTGCATGCT ATTTGGTCAC AAAACACTCA 
TGGAAGGGAA AGTACAAACG GGTGTTTTCT ATCGGGACAC TGGCCATCAC CACTTACAAT 
CCTAATACCT TGGAGATTAC CAATCAATGG CTCTACGAGG ACTTCTTATC AGTGAAACCA 
CTTCGAAATG GAGGTTCAAA TGATTCAAAA CAGGATGAAT ACAAGATTCA TGTTAGGAAT 
CGTGGAAAAA ACAATGATAT GCGTTTTTCA TCTGATTATA CCACCGATAT ACTTACACAT 
TGTCTGCAAT TCAGCACCAA GTTCGCTGAT AAGAACTTTG AACCTTTGAC GGTAACTGCA 
TTCAAACAAA GTTGGAGTGA TCGTCGAATT CCCGTAACTC TACGAGCAAA TGCTTCTTGT 
TTGGAGCAAA TTGATAATCG TGGAGTCGTC GTTCAAAGTT ATCCGTACAA AAATATTCGA 
AGTATTGGAA AAGTGTCCGA TTGTCCCGGT GGTTTTGTGG TTGATGTGGG AGAACATAGA 
CGGAGGCACA TGTTCGCTTC TTCAAACAAC GAAGAACTAG TGAAAGAGAT TCGTCGTTTA 
GCATCTGATA ATATCGGAAT TATCGTTCCA ATTGCTAAAG AGCAGCTAAC ACTCGAAGAT 
TTTATGAGAA CTCGACTCGG ATTGTGCAGT CGAGATGAAG AGCTCACTTC ATATGCTGAA 
TTCAAAATTT CCAAAATTAC TCGAAGAAAT GAGATGCCTG TTCGACGGCT TCTATGTCTC 
AGTGAGACGT GTATTATTGA AAGAGACTTG GCTACTTATG CAGTGATTTG TGCAACTCCA 
CTGAAACATA TCGTTTGCCT TGTTCGAAGT GAAAAAGATC CCCAACAATT CATTGTTGAA 
TACGAAAATG GAGATGGGCG AGCATATGTT GCTGCTGAAA GAGATTTGAT CTTAGCCTCA 
CTCTTAGATG GAATTCGTGC TTCTGGAAAT AATGAAGTGT TCGTTTGTGG ACACAGATTT 
GAAAGGAATT TGAGAGTGAT CCCATTCTCA ACGAATTTGG ATGAAGATAG TGAATCTCAA 
TGTATGAAGC ATATTATTGC ACCTCCACCT GGACTTCGTC GTTGTGATTT GATCCGTCGC 
TTCAACGCAA ATGTTCCATA TTCTGGTCTT CGATTTTCAA AGTCGCACGA AGGATTCTTC 
TCAGAAAACA AAGGAAAAGT TATTGTAAAT GCAATTGAGG CAGTTCTAAT GGAGAATTAT 
ACAAAAGACG ATAAGGAGTA CAAGCACAAA ACTGAAGCTC AGTTACAGTG TTTACGAAGA 
CTTTTTGCGT CGAAAAGTGG ATTCCAGGCA TTTACAGAAG TTAATGGTGT TCGTGAGAAG 
CTTGGGAGTC TTGTGGTTCG AGTTCTATCT TGGAAATCTG AATCAATTGA TCATTCGACG 
GTTGAAGCTC TTTGTGCATT GATGTATCCA ATGCATGATC AATATGAACT TAGAATAGAG 
CAACTGAACA AGCAGAGTCT GATGAGCAGC CCAAAATTTG TGGAGAACTT ACTCGATTTG 
ATTGTTCTTC ACGTGGATCG TTCAACTGGA TGGCTTGTGA TTGCATCAAT GCTCAATTTC 
CTCACATTTT CCGTTTGCTC TCCGTACTCT GAAACCACCG CTGGAGATAC TTTCGATCAT 
ATTCTGAAAC TCGTATCTCT CCGAGGGCGC AGCTTTTTCC GTTTATTCCA ATGTCCCAGT 
ATGACAATTG TTAAAGGTGC TGGAATGGTT ATGAGAGCAA TTATCGAAGA AGCTGATGTT 
GAAACTTCAA AATCAATGCA AATGTTAGCA CTATCAGAAG GAGCATTCTT GACACATTTG 
TTCATGTCAT TATTGTCAAC TGGAAAAGAT TTACGAGTAA TGACAAATAA ACAATTGTCT 
GGACATTTGA TAAGTCTTTG GATAGCTGAT AATCAACAGG CTAATGATTT GTTAATTAGG 
TGCCTGCCCC GTGGTCTATT GAACTATATG GAGTCAGACG AAAAAGTTCC AGTAAACGAG 
AAAGATCTTC TCATTGTTCG AAATAACTTT GATGCAGCAT CAAATGAAAC TAAGCAGAAT 
GCGATGAAGG AGAAGTTTGA CCAGCTTCGA GTTACTGCTG AAGCTGGTTT GGAAAGATTT 
GTGCAGCACT GGGATCTCGA ACAAAAACTC AATTTTCTTC CAAAACGAGT TATTGATGAG 
AAACAACAAC AAAGACAGCA ACCGGTTGTC CTGAGAAAAC GAAGAAATCG AGTACGGCCA 
AATGTTAATT GGAAACTATT TGCATATCAA TTCTCGAAGG ATCACTCTCA GGCAGATTTA 
ATTTGGAATG AGAAGACGCG AGAAGAGTTC CGTCATGCAA TGGATACAGA AACACGAGCT 
CTTATCAGTG AAAAAGAACA AGCACCAACT GGATTGCCGA TTGCTTGGAA TCATACTGAG 
TTCCAAATCA GATATCCATC ACTTTTAGAA GAAATTAAAA TTGGAGATTA TTACCTTCGA 
TTGCTTCTGA TTGAGGCAGA CGAAAATGCG ACTCCGATTC ATAATCCGCT GGAGTTCTTC 
AACAACGTCT ATCATCGTTT CCTACTTTCC ACTAAAGTTG ACATGAAATG TCTGTGCCTT 
CGTGCAATGG CTATCACCTA TTCTCGACAT CATATGACAA TCGGAGCATT TCAGGATTCA 
AAGTATTTCG TAGAAATGCT TCAAAAATGC ATAAATCCGT TGGAAAGAGA TCATCTTGTG 
CTTCTGTTAT CGAAACTAGC ACTGAATAAG GATAATGTTC GAGAACTTAT AATATCCAAT 
ATTCTTCCAC TTTTGGTAGA TCTTTGTGTT CTTGCACATT TACACGTGCA GCGAGCAAAA 
GTTCAAAATC AGACAAATGT GATTGAGGCA AGTGCCGAAC AAATGGCTGA AGGAGGTTCT 
GAAGAATGGT ATTATCATGA TAAGGATGCT AAACAAGTCG GACCATTGTC ATTTGAGAAA 
ATGAAGTCTC TTTACACGGA GAAGACTATT TTTGAGAAAT CTCAAATTTG GGCAGCTGGA 
ATGGACAAGT GGATGAGTTT GGCTGCAGTT CCACAATTCA GATGGACTGT TTGTCAGCAA 
AAAGATCAGC CGAACGAGAT TAATACAGGA AAAGGACCAT TCCAGGCAAC TGTCATGAAC 
TTCACAGATC TCAGTGTTCT TTGTTTAGAT ACTATTCTTC AGATGTGCGA ATTTTTCCCA 
TCAAGGGATT CTCATGATTG TGTGGTCCGT CCGATGCCAT CTGTCAAGAA GCAGCTCACT 
GAGCCAGTAC TCCTTTATCA ACTCGTTCAA CTTCTTCTAA CCTATGAACC ACAGATTGTG 
CAGAGAGTTG CTCTACTACT GTATCTTGTA ATGCAGGATA ACCCATTTCT TCCACGACTT 
TATCTTTCTG GTGTCTTCTA TTTTATTCTT ATGTACAATG GATCAAATGT TCTTCCGATT 
GCTCGATTCC TTCACTATAC CCACATGAAA CAAGCATTCC GTTCTACACT TCCACAATTT 
GAGGGACAAC GGCAATCAGT TCTTGCCACT CTTCTTCCAG AAGCCGCCAC ATTCTATTTG 
GAACAATATG GACCTGAAAA GTATGCCGAA GTGTTCCTTG GAGAATTCGA TAATCCCGAA 
ATCATTTGGA ATACGGCGAT GCGTCGACAT TTGATTGAAC GAATCGCTGT TCATGTTGCA 
GATTTCTCGC ATCGATTAAC ATCAAATGTT CGAGCTTTAT ATCAATTCTG TCCAATTCCT 
TTGATCGATT ATCCAGAACT TGCTCAGGAA CTCTTCTGTC ATGTTTATTA TCTTCGACAC 
TTGTGTAATA AGCAAAGATT CCCTGATTGG GAAATTAGGG ATCCGATTCC GTTCCTTCGA 
TGTTGCCTTG CCACATGGTA TAATGAGCTA GAGAAGAAGC CGGCGACGAT GTCAGTCGAT 
CTTGCAAGAG AGATTTTATC AGTTGATTTG ACTAACGAAG AACACCGAAA ACCTGCATTC 
ATACGTCGTC AATATTACAA ACTCGCTGCT AAATACCATC CTGACAAGAA TCCAGAAGGA 
AGAGAAATGT TCGAACGAAT AAATGCAGCT TATGAACTAT TGAGCAGTGA AACTGCAAAT 
AATTCAGGAA TGCCGGATTC GCATCGAATT GTGCTTTGTT TACAGGCACA ATCCATCATT 
TATAGTAGAT ATAGTCAAGA ATTATCCGAA TACAAATACG CTGGTTACTC TCAACTCATT 
AAAACTATCG ATTTGGAAGC AAAAGACGAA GCATTGTTCA TAAAAGGAGG TGGAGACCTG 
TTGTCAGCTG CTATTGAACT TGCTAATTAT ACACTTATTA GTAGTGCTTT GAATGCTGAA 
CAATTGAGAA GAGATAATGG ATTAGAGGCA TTAGTAACTG CATTTGATCG ATGTGTTCCA 
ATGGTAACAA TGAGTTCATT ACCTGATGAT ATGGCTGTTC GAGTTTGTAT TCATGTTTGT 
GATTGTTTTG CCACTGCTGC AACATTTGAA GCGTGTAGGC AGCGACTGAT GGAGATGCCA 
TCCATATTTG GAGCCCTCAC TAGATTACTT CAATTTTCGA ACTTACCCCG TCTCTCCACT 
GCAGCAGCTC AATGTATTCG TGCAATGGCA GTTGACACAC TTCTTCAATT CCAACTTTTC 
CAAACTGGTG TTCTATGGCA ATTGGTACCA CATCTCTTTC ATTTCGATTA TACATTGGAT 
GAAGGAGGTG TTCAACATTC AGAGGATTCT AATAAACAAT CACTCGCTAA TTCATTAGCT 
CGTTCTTCGT GTGAAGCTCT TGCAGCTCTG GCTGGATTCC GTGAGAATAC ACCGGATAAT 
GATGGAGTTC AGGCAAGCCT TCGAGCTCTA CTTACTCCTT ACATCTGTCG ATGCATGAAG 
CTTGAAACAA ATGATATGGT TCTGAAAACG CTGAATAGTA ATATGGAAAA TCCATATATG 
ATCTGGGATA ATGGAACAAG AGCTGAAGTT CTCGAATTTG TGGAGAGACA TCGAACTTCT 
AATGAGCCTA CGAGTGAGCT GTACGGAGCC GAATTCGAGT TATCTGTGCA CAAAAATGAG 
CTTATCGTTG GAGACATATT CATCAGAATC TACAATGAAC AGCCAACATT TGCTCTTCAC 
GAACCCAAAA AAGTTGCAAT CGATCTTTTG GATTACATCA AAAAACACTC GGCAGAACTA 
ACCGGAGCTC CAAAACCTAA AGCAATTTCG GATGATTTGA TTGAAATTGA CTGGGGAAAT 
GGATCACAGG CTAATGGAAA CTCATTGGAC ACAGAAACAA AAGTTCTCAT GACAATGACT 
GCATTAGCTA ATTTAGTTTC AGCGAATCCA GGTGTTGAAA TTCTACTAAT TGGAAACTTC 
CCACTTCTTA TCACTTATCT ACGTTGTCGA AAACATCCGA AGCTTCAAAT TGCTGCACTT 
CAAGTCATTC TGTTGGCTGC AGCTAATAAG GAATGTGTAA CTGATTTGGC CACGTGTAAT 
GTCACTACAA CACTTTTCAC ACTTTTACGT GATCAGCCGA AGATGATAGC AAGAGTATTA 
GATGTTCTGA TTGCACTTTC ATCAAATGGA CAAATTGGAA AAGAAATGTT AGAACATGGA 
GGACTTATGT ATATTCTATC AATTCTTTGT TTGACAAATT CTGATCAAGG CCAAAGACTG 
CAGGCAGCCG AGCTTTTGGC CAAACTTCAA GCTGATAAGC TTACTGGGCC AAGATGGACA 
CGATTTATTA TCAAGTTTTT GCCAGAAATA TTTGCAGATT CATTGAGAGA TTCGCCCAAT 
ACAGCCCTTC AAATGTTCGA TTCATCAAGC GAAAATCCAG AGCTTATTTG GAATGAAGTA 
ACGCGTCAAA AAGTGAAAGG AATCATTGAT ACAGAAGTCG GAAAACTCTA TGAACAACAC 
ATCAAAGATC CAACTGTCAA ATGGAATACG ACTTCATTGG CTGACAAGAG TTGTGCCTAT 
GGTGATAGTA TTAATGGTGA ACTTGTTGTT GGAGGTGTTT TTGTACGATT ATACGTCGAA 
AATCCAGGAT TCAATGTCAG ACATCCAAAA TTATTCTCAA CAGAGCTCAT CGAAAAAGTG 
CTTGAACTGA TGAGTCGTCC AACTCCAGAA CTCGATTTGG TCACAAAAGC CTTTGTTGAA 
CTGGTCCGTC ATCATCCGAA TACTGCTGAT CAATTGCCAT CTCAAGGATA TCTACCACAG 
TTCTGCACTG CAATGTGTCT TCAAAACACA TCTGCGTCTC GTTCAGCAAT TCTAATTCTT 
CAAGAATTGA GCGAAAATCA ATTCTGTTGT GATGCTCTTT CTCAGCTGCC ATGTATTGAT 
GGAATAATGA AATCAATGAA GAATCAACCA AGTTTGATGA GAGAATCGGC GCACGCTTTA 
AAATGTCTCA TGAAGAGAAA TACAGGAGAA TTGGCTCAAC AAATGCTCTC ATGTGGAATG 
GTTCCATATT TGTTACAAGT TTTGGATAGT TCAATGAATG GAGTATCAAA CGGAGCAGCT 
GCTCGTGCCG AGATTGTTGA CGCATTAAAA AGCGCAATTT TAGATCTGAA AGTTGGTCAG 
AAGATTGCTG AGATTTTAGA CAAGAGTCCC GTCTGGGCAC AGTTTAAAGA TCAACGACAT 
GATTTATTCT TGCCAGAAGC GAGAACTCAA GCTATTACAG GAGGTCCAAC TGGTGTTGCT 
GGATATCTAA CAGAAGGAAT GTTCAATCCA CCACCAATGA GCAATCAGCC ACCACCAATG 
CATCAAACAT CTGGAGATTT CCAACCACCA CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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rme-8 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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