drh-3 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	drh-3
Entrez Gene ID	172546
Full Name	Dicer Related Helicase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_059760.5	NP_492161.3	Dicer Related Helicase
NM_059760.6	NP_492161.3	Dicer Related Helicase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that DRH-3 specifies 22 bp RNAs by dimerization of the helicase domain, a process mediated by ATPase activity and the N-terminal domain.
Title: Dicer-related helicase 3 forms an obligate dimer for recognizing 22G-RNA.

DRH-3 is required for the biogenesis of virus-derived secondary, but not primary, siRNAs.
Title: Homologous RIG-I-like helicase proteins direct RNAi-mediated antiviral immunity in C. elegans by distinct mechanisms.

ATPase activity of DRH-3 is stimulated only by double-stranded RNA. DRH-3 is a robust RNA-stimulated ATPase with a k(cat) value of 500/min when stimulated with short RNA duplexes.
Title: Double-stranded RNA-dependent ATPase DRH-3: insight into its role in RNAsilencing in Caenorhabditis elegans.

the drh-3 gene is essential for the development of germ-lines by maintaining chromosomal integrity in C. elegans.
Title: Dicer-related drh-3 gene functions in germ-line development by maintenance of chromosomal integrity in Caenorhabditis elegans.

The following drh-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the drh-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335298	NM_059760.6 Latest version!	Caenorhabditis elegans Dicer Related Helicase (drh-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa335298	NM_059760.5	Caenorhabditis elegans Dicer Related Helicase (drh-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

drh-3 ( NM_059760.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492161.3 Caenorhabditis elegans Dicer Related Helicase (drh-3), partial mRNA.

Price & Availability↑

CloneID	OCa335298
Clone ID Related Accession (Same CDS sequence)	NM_059760.5 , NM_059760.6
Accession Version	NM_059760.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Dicer Related Helicase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_059760.4. COMPLETENESS: incomplete on both ends.

ATGCAACCAA CTGCAATACG TTTAGAAGAT TACGACAAAT CGAAGCTTCG CCTTCCGTTT 
GAGTCTCCAT ATTTTCCAGC GTATTTTCGT CTTCTCAAAT GGAAATTCCT TGATGTATGC 
GTGGAGAGTA CACGAAACAA CGATATCGGA TATTTCAAAC TATTTGAATC GCTGTTCCCG 
CCAGGAAAAT TGGAAGAAAT TGCCAGAATG ATCATTGACG AGCCAACTCC AGTTTCTCAT 
GACCCGGACA TGATTAAAAT CAGAAATGCC GATCTCGACG TTAAAATCAG AAAACAGGCA 
GAAACATACG TTACATTGCG TCACGCACAT CAGCAGAAAG TTCAAAGGAG AAGATTCTCG 
GAATGTTTTT TGAACACTGT TTTGTTCGAT GAGAAGGGAC TCAGAATTGC TGACGAAGTG 
ATGTTTAACT ACGATAAAGA ACTTTATGGA TACTCACACT GGGAAGATCT TCCTGATGGC 
TGGTTAACTG CTGAGACTTT CAAAAATAAA TTCTACGATG AAGAGGAAGT TACCAATAAT 
CCATTTGGAT ATCAGAAGCT TGATAGAGTG GCTGGAGCTG CACGTGGAAT GATTATTATG 
AAGCATCTGA AATCGAATCC ACGTTGTGTT AGTGAAACTA CAATTCTCGC TTTTGAAGTA 
TTTAACAAAG GTAATCATCA ACTATCAACT GATTTGGTCG AAGATTTGCT AACCGAAGGA 
CCAGCGTTCG AGCTGAAAAT CGAGAATGGA GAAGAGAAAA AATATGCTGT GAAGAAATGG 
AGTCTTCATA AGACTCTCAC CATGTTTCTC GCAATCATCG GATTCAAATC GAATGACAAA 
AAAGAGAAAA ATGAACACGA AGAGTGGTAT TACGGATTTA TTGATGCAAT GAAGAATGAT 
CCAGCTAATC GAGCGGCTCT CTACTTTTTG GATAAGAACT GGCCAGAAGA GCTTGAAGAA 
CGAGAGAAAG AACGTGACCG TATCCGCCTT ACCCTACTGA AAAGTCAGCG GACTAACGAG 
GAAGCTGTCG GCGAGGATGT TTGCACGACA ATACGTCCAC AACCGAAAGA CAGCGGCTAC 
AATCCAGATG CGGTGGTTAC TGAACTTGTA TTGCGTACTT ATCAAGAAGA ACTTGTCCAA 
CCGGCTCTCG AAGGAAAGAA TTGTGTTATT GTTGCTCCGA CTGGTTCTGG TAAAACAGAA 
GTGGCAATTT ATGCGGCTCT CAAACATATT GAAGAACGAA CATCCCAAGG AAAGCCTTCC 
AGAGTTGTGC TCCTTGTGCC GAAAATCCCG CTTGTCGGTC AACAAAAAGA CAGATTCCTG 
AAGTATTGTA ACGGAATGTA CGAAGTTAAT GGTTTTCATG GATCCGAGAG CAGTGTCAGT 
GGAACAGGAC GTCGTGATGA AGTAATTGCT ACTCATGTAT CAGTTATGAC TCCTCAAATT 
CTGATCAACA TGCTTCAATC GGTACGTCAA AATGAGCGTT TGTATGTGAG TGATTTCTCA 
ATGATGATCT TCGATGAAGT ACATAAAGCT GCAAAAAATC ATCCATATGT GTTGATTAAT 
CAAATGGTTC AAGAATGGAA GTATGAAAAA CCACAAATCA TTGGTCTGAC AGCTTCATTG 
AGTGTCAAAG TTGATGGACA AAAAGACGAG AATCAGATGC TTAATGATAT TTATAATATG 
CTCGCATTGA TCAACGCTCC ACATTTGAGT ACAATCACTC GCCAATCGAG CATAGATGAG 
CTCAATGAGC ATGTTGGAAA ACCAGATGAT TCTGTCGAAC TATGCTTGCC TGCCAAAGAA 
AATATCCTTC GAGATTACAT CGAACGATAC CTTAACCACG CCCATGGCAA ATTTCTCGAA 
GAATTAGCAA GCATGTCTAA ATCAACTGGC CGCAACAACA CTATCCCACC GAATATGATT 
AACACGTTTA AAAAGAATCA ACCGAAGAAT TATGAGTACT ACGATTCATT ACTTCAAGGT 
ATTATTCAAG AGTTGAACAA ATTGAATGTT CCTGAGAAAT GGAATTCTCA AACTTGGGCG 
AAATACATGA AGGTATATCT CGAAGCTCGC GGAATCGTTG ATCTGATGCC TGCGATGGTT 
GCATTCAAAT ACATGGAAAA AGCTATTGGG AAATTGAATG AAAGTCATTC AGAGACAGTC 
GAATACAGCA CCTTCATCAA AGACCATGAT ACTTTGAAAC AAACAATTCA ATCCGTTGAA 
CCAGAAATTG TTCTACGTCT CAAGAATACA CTCACCAATC AGTTCCACGT GGCTCCTGAA 
TCTCGAGTTA TTATTTTCGT AACACAAAGA AGCACCGCTC AACGTGTCAG TGATTTCTTG 
AACGAATCTA AAGTTTTAGA TCAATTTGGT AATTATGGTG AACAAATGGT TGGATATGTT 
CTCGGAACAA ACAAACAGGG CGCTGTACAG CAGACTTCAC AAGAACAACA ATTGACACTT 
GATAAATTCA ACAATGGAAG ACTTAAAGTA ATTGTGGCAA CGTCAGTTGT TGAAGAAGGT 
CTCGATGTTA CTGCATGTAA TCTTATTATC AAGTACAACT GTTCATCAGG ATCAGCCATT 
CAACTTGTTC AACAAAGAGG TCGTGCACGT GCCAAAAACT CTCGTTCTGT GCTTTTATCA 
GTGAAGAGTA GTATCAATGA AACCGAGACT AACGCGTTAA TCTCTGAAAA GTATATGAGA 
CTTTGTGTGA AGAAGATCAC AGAGAATGGC GAGAAACAAT TGGCTGCAGA AGTGAAACGA 
GTCGCTGAAC TAAATGCGGC TGAAAGGAAA CGAAATCTGG AAGAGCAACT CAATTTGAGA 
TTACGACATG AGAATAAGAT TTATAAATTG ATGTGCAGCA ATTGTTCAAA AGAGTTCTGC 
AAGAGTATTT ATATCAAAAA AGTGTTCTCG AATTACATGG TGTTCGATCC AAGTGTATGG 
AGATTTTTGC ATGTTGAATC GAAGCGAAAG GTGAGTAAGT ACTTGAGTGA GGACAATCAA 
CCACTCAGTG ATATCAAATG CTTCCATTGC AAGTTGGATG TTGGAAGAGC TTACAAAATA 
AGAGGAACCT ATTTGCCGCA ACTCAGTGTC AAGGCGTTGA CATTTGTTCA AGAATCAGAC 
TATTCCTCGA TGACAAAAGC AAAATGGTCG GATGTGGAAC AGGATCTGTT TTATATTTCA 
GAAGCAATCG AGGATGACTT TAGAATCATG TTGAATGCGC TCTCCGATAC TGAAGAAAAT 
ATCGAAAAGA AGATTGTTCT TGATTTGGAC TCACGACAAC ACAATAAGCA ACTCGAGATG 
AAGAGATTCC ATATTCAACA AGAACCACCA ACGAAGGGAG TTGCACCAGA AGCTCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492161.3
CDS	1..3360
Translation	MQPTAIRLEDYDKSKLRLPFESPYFPAYFRLLKWKFLDVCVESTRNNDIGYFKLFESLFPPGKLEEIARMIIDEPTPVSHDPDMIKIRNADLDVKIRKQAETYVTLRHAHQQKVQRRRFSECFLNTVLFDEKGLRIADEVMFNYDKELYGYSHWEDLPDGWLTAETFKNKFYDEEEVTNNPFGYQKLDRVAGAARGMIIMKHLKSNPRCVSETTILAFEVFNKGNHQLSTDLVEDLLTEGPAFELKIENGEEKKYAVKKWSLHKTLTMFLAIIGFKSNDKKEKNEHEEWYYGFIDAMKNDPANRAALYFLDKNWPEELEEREKERDRIRLTLLKSQRTNEEAVGEDVCTTIRPQPKDSGYNPDAVVTELVLRTYQEELVQPALEGKNCVIVAPTGSGKTEVAIYAALKHIEERTSQGKPSRVVLLVPKIPLVGQQKDRFLKYCNGMYEVNGFHGSESSVSGTGRRDEVIATHVSVMTPQILINMLQSVRQNERLYVSDFSMMIFDEVHKAAKNHPYVLINQMVQEWKYEKPQIIGLTASLSVKVDGQKDENQMLNDIYNMLALINAPHLSTITRQSSIDELNEHVGKPDDSVELCLPAKENILRDYIERYLNHAHGKFLEELASMSKSTGRNNTIPPNMINTFKKNQPKNYEYYDSLLQGIIQELNKLNVPEKWNSQTWAKYMKVYLEARGIVDLMPAMVAFKYMEKAIGKLNESHSETVEYSTFIKDHDTLKQTIQSVEPEIVLRLKNTLTNQFHVAPESRVIIFVTQRSTAQRVSDFLNESKVLDQFGNYGEQMVGYVLGTNKQGAVQQTSQEQQLTLDKFNNGRLKVIVATSVVEEGLDVTACNLIIKYNCSSGSAIQLVQQRGRARAKNSRSVLLSVKSSINETETNALISEKYMRLCVKKITENGEKQLAAEVKRVAELNAAERKRNLEEQLNLRLRHENKIYKLMCSNCSKEFCKSIYIKKVFSNYMVFDPSVWRFLHVESKRKVSKYLSEDNQPLSDIKCFHCKLDVGRAYKIRGTYLPQLSVKALTFVQESDYSSMTKAKWSDVEQDLFYISEAIEDDFRIMLNALSDTEENIEKKIVLDLDSRQHNKQLEMKRFHIQQEPPTKGVAPEAQ

Target ORF information:

RefSeq Version	NM_059760.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Dicer Related Helicase (drh-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059760.5

ORF Insert Sequence:

ATGCAACCAA CTGCAATACG TTTAGAAGAT TACGACAAAT CGAAGCTTCG CCTTCCGTTT 
GAGTCTCCAT ATTTTCCAGC GTATTTTCGT CTTCTCAAAT GGAAATTCCT TGATGTATGC 
GTGGAGAGTA CACGAAACAA CGATATCGGA TATTTCAAAC TATTTGAATC GCTGTTCCCG 
CCAGGAAAAT TGGAAGAAAT TGCCAGAATG ATCATTGACG AGCCAACTCC AGTTTCTCAT 
GACCCGGACA TGATTAAAAT CAGAAATGCC GATCTCGACG TTAAAATCAG AAAACAGGCA 
GAAACATACG TTACATTGCG TCACGCACAT CAGCAGAAAG TTCAAAGGAG AAGATTCTCG 
GAATGTTTTT TGAACACTGT TTTGTTCGAT GAGAAGGGAC TCAGAATTGC TGACGAAGTG 
ATGTTTAACT ACGATAAAGA ACTTTATGGA TACTCACACT GGGAAGATCT TCCTGATGGC 
TGGTTAACTG CTGAGACTTT CAAAAATAAA TTCTACGATG AAGAGGAAGT TACCAATAAT 
CCATTTGGAT ATCAGAAGCT TGATAGAGTG GCTGGAGCTG CACGTGGAAT GATTATTATG 
AAGCATCTGA AATCGAATCC ACGTTGTGTT AGTGAAACTA CAATTCTCGC TTTTGAAGTA 
TTTAACAAAG GTAATCATCA ACTATCAACT GATTTGGTCG AAGATTTGCT AACCGAAGGA 
CCAGCGTTCG AGCTGAAAAT CGAGAATGGA GAAGAGAAAA AATATGCTGT GAAGAAATGG 
AGTCTTCATA AGACTCTCAC CATGTTTCTC GCAATCATCG GATTCAAATC GAATGACAAA 
AAAGAGAAAA ATGAACACGA AGAGTGGTAT TACGGATTTA TTGATGCAAT GAAGAATGAT 
CCAGCTAATC GAGCGGCTCT CTACTTTTTG GATAAGAACT GGCCAGAAGA GCTTGAAGAA 
CGAGAGAAAG AACGTGACCG TATCCGCCTT ACCCTACTGA AAAGTCAGCG GACTAACGAG 
GAAGCTGTCG GCGAGGATGT TTGCACGACA ATACGTCCAC AACCGAAAGA CAGCGGCTAC 
AATCCAGATG CGGTGGTTAC TGAACTTGTA TTGCGTACTT ATCAAGAAGA ACTTGTCCAA 
CCGGCTCTCG AAGGAAAGAA TTGTGTTATT GTTGCTCCGA CTGGTTCTGG TAAAACAGAA 
GTGGCAATTT ATGCGGCTCT CAAACATATT GAAGAACGAA CATCCCAAGG AAAGCCTTCC 
AGAGTTGTGC TCCTTGTGCC GAAAATCCCG CTTGTCGGTC AACAAAAAGA CAGATTCCTG 
AAGTATTGTA ACGGAATGTA CGAAGTTAAT GGTTTTCATG GATCCGAGAG CAGTGTCAGT 
GGAACAGGAC GTCGTGATGA AGTAATTGCT ACTCATGTAT CAGTTATGAC TCCTCAAATT 
CTGATCAACA TGCTTCAATC GGTACGTCAA AATGAGCGTT TGTATGTGAG TGATTTCTCA 
ATGATGATCT TCGATGAAGT ACATAAAGCT GCAAAAAATC ATCCATATGT GTTGATTAAT 
CAAATGGTTC AAGAATGGAA GTATGAAAAA CCACAAATCA TTGGTCTGAC AGCTTCATTG 
AGTGTCAAAG TTGATGGACA AAAAGACGAG AATCAGATGC TTAATGATAT TTATAATATG 
CTCGCATTGA TCAACGCTCC ACATTTGAGT ACAATCACTC GCCAATCGAG CATAGATGAG 
CTCAATGAGC ATGTTGGAAA ACCAGATGAT TCTGTCGAAC TATGCTTGCC TGCCAAAGAA 
AATATCCTTC GAGATTACAT CGAACGATAC CTTAACCACG CCCATGGCAA ATTTCTCGAA 
GAATTAGCAA GCATGTCTAA ATCAACTGGC CGCAACAACA CTATCCCACC GAATATGATT 
AACACGTTTA AAAAGAATCA ACCGAAGAAT TATGAGTACT ACGATTCATT ACTTCAAGGT 
ATTATTCAAG AGTTGAACAA ATTGAATGTT CCTGAGAAAT GGAATTCTCA AACTTGGGCG 
AAATACATGA AGGTATATCT CGAAGCTCGC GGAATCGTTG ATCTGATGCC TGCGATGGTT 
GCATTCAAAT ACATGGAAAA AGCTATTGGG AAATTGAATG AAAGTCATTC AGAGACAGTC 
GAATACAGCA CCTTCATCAA AGACCATGAT ACTTTGAAAC AAACAATTCA ATCCGTTGAA 
CCAGAAATTG TTCTACGTCT CAAGAATACA CTCACCAATC AGTTCCACGT GGCTCCTGAA 
TCTCGAGTTA TTATTTTCGT AACACAAAGA AGCACCGCTC AACGTGTCAG TGATTTCTTG 
AACGAATCTA AAGTTTTAGA TCAATTTGGT AATTATGGTG AACAAATGGT TGGATATGTT 
CTCGGAACAA ACAAACAGGG CGCTGTACAG CAGACTTCAC AAGAACAACA ATTGACACTT 
GATAAATTCA ACAATGGAAG ACTTAAAGTA ATTGTGGCAA CGTCAGTTGT TGAAGAAGGT 
CTCGATGTTA CTGCATGTAA TCTTATTATC AAGTACAACT GTTCATCAGG ATCAGCCATT 
CAACTTGTTC AACAAAGAGG TCGTGCACGT GCCAAAAACT CTCGTTCTGT GCTTTTATCA 
GTGAAGAGTA GTATCAATGA AACCGAGACT AACGCGTTAA TCTCTGAAAA GTATATGAGA 
CTTTGTGTGA AGAAGATCAC AGAGAATGGC GAGAAACAAT TGGCTGCAGA AGTGAAACGA 
GTCGCTGAAC TAAATGCGGC TGAAAGGAAA CGAAATCTGG AAGAGCAACT CAATTTGAGA 
TTACGACATG AGAATAAGAT TTATAAATTG ATGTGCAGCA ATTGTTCAAA AGAGTTCTGC 
AAGAGTATTT ATATCAAAAA AGTGTTCTCG AATTACATGG TGTTCGATCC AAGTGTATGG 
AGATTTTTGC ATGTTGAATC GAAGCGAAAG GTGAGTAAGT ACTTGAGTGA GGACAATCAA 
CCACTCAGTG ATATCAAATG CTTCCATTGC AAGTTGGATG TTGGAAGAGC TTACAAAATA 
AGAGGAACCT ATTTGCCGCA ACTCAGTGTC AAGGCGTTGA CATTTGTTCA AGAATCAGAC 
TATTCCTCGA TGACAAAAGC AAAATGGTCG GATGTGGAAC AGGATCTGTT TTATATTTCA 
GAAGCAATCG AGGATGACTT TAGAATCATG TTGAATGCGC TCTCCGATAC TGAAGAAAAT 
ATCGAAAAGA AGATTGTTCT TGATTTGGAC TCACGACAAC ACAATAAGCA ACTCGAGATG 
AAGAGATTCC ATATTCAACA AGAACCACCA ACGAAGGGAG TTGCACCAGA AGCTCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

drh-3 ( NM_059760.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492161.3 Caenorhabditis elegans Dicer Related Helicase (drh-3), mRNA.

Price & Availability↑

CloneID	OCa335298
Clone ID Related Accession (Same CDS sequence)	NM_059760.5 , NM_059760.6
Accession Version	NM_059760.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Dicer Related Helicase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059760.5.

ATGCAACCAA CTGCAATACG TTTAGAAGAT TACGACAAAT CGAAGCTTCG CCTTCCGTTT 
GAGTCTCCAT ATTTTCCAGC GTATTTTCGT CTTCTCAAAT GGAAATTCCT TGATGTATGC 
GTGGAGAGTA CACGAAACAA CGATATCGGA TATTTCAAAC TATTTGAATC GCTGTTCCCG 
CCAGGAAAAT TGGAAGAAAT TGCCAGAATG ATCATTGACG AGCCAACTCC AGTTTCTCAT 
GACCCGGACA TGATTAAAAT CAGAAATGCC GATCTCGACG TTAAAATCAG AAAACAGGCA 
GAAACATACG TTACATTGCG TCACGCACAT CAGCAGAAAG TTCAAAGGAG AAGATTCTCG 
GAATGTTTTT TGAACACTGT TTTGTTCGAT GAGAAGGGAC TCAGAATTGC TGACGAAGTG 
ATGTTTAACT ACGATAAAGA ACTTTATGGA TACTCACACT GGGAAGATCT TCCTGATGGC 
TGGTTAACTG CTGAGACTTT CAAAAATAAA TTCTACGATG AAGAGGAAGT TACCAATAAT 
CCATTTGGAT ATCAGAAGCT TGATAGAGTG GCTGGAGCTG CACGTGGAAT GATTATTATG 
AAGCATCTGA AATCGAATCC ACGTTGTGTT AGTGAAACTA CAATTCTCGC TTTTGAAGTA 
TTTAACAAAG GTAATCATCA ACTATCAACT GATTTGGTCG AAGATTTGCT AACCGAAGGA 
CCAGCGTTCG AGCTGAAAAT CGAGAATGGA GAAGAGAAAA AATATGCTGT GAAGAAATGG 
AGTCTTCATA AGACTCTCAC CATGTTTCTC GCAATCATCG GATTCAAATC GAATGACAAA 
AAAGAGAAAA ATGAACACGA AGAGTGGTAT TACGGATTTA TTGATGCAAT GAAGAATGAT 
CCAGCTAATC GAGCGGCTCT CTACTTTTTG GATAAGAACT GGCCAGAAGA GCTTGAAGAA 
CGAGAGAAAG AACGTGACCG TATCCGCCTT ACCCTACTGA AAAGTCAGCG GACTAACGAG 
GAAGCTGTCG GCGAGGATGT TTGCACGACA ATACGTCCAC AACCGAAAGA CAGCGGCTAC 
AATCCAGATG CGGTGGTTAC TGAACTTGTA TTGCGTACTT ATCAAGAAGA ACTTGTCCAA 
CCGGCTCTCG AAGGAAAGAA TTGTGTTATT GTTGCTCCGA CTGGTTCTGG TAAAACAGAA 
GTGGCAATTT ATGCGGCTCT CAAACATATT GAAGAACGAA CATCCCAAGG AAAGCCTTCC 
AGAGTTGTGC TCCTTGTGCC GAAAATCCCG CTTGTCGGTC AACAAAAAGA CAGATTCCTG 
AAGTATTGTA ACGGAATGTA CGAAGTTAAT GGTTTTCATG GATCCGAGAG CAGTGTCAGT 
GGAACAGGAC GTCGTGATGA AGTAATTGCT ACTCATGTAT CAGTTATGAC TCCTCAAATT 
CTGATCAACA TGCTTCAATC GGTACGTCAA AATGAGCGTT TGTATGTGAG TGATTTCTCA 
ATGATGATCT TCGATGAAGT ACATAAAGCT GCAAAAAATC ATCCATATGT GTTGATTAAT 
CAAATGGTTC AAGAATGGAA GTATGAAAAA CCACAAATCA TTGGTCTGAC AGCTTCATTG 
AGTGTCAAAG TTGATGGACA AAAAGACGAG AATCAGATGC TTAATGATAT TTATAATATG 
CTCGCATTGA TCAACGCTCC ACATTTGAGT ACAATCACTC GCCAATCGAG CATAGATGAG 
CTCAATGAGC ATGTTGGAAA ACCAGATGAT TCTGTCGAAC TATGCTTGCC TGCCAAAGAA 
AATATCCTTC GAGATTACAT CGAACGATAC CTTAACCACG CCCATGGCAA ATTTCTCGAA 
GAATTAGCAA GCATGTCTAA ATCAACTGGC CGCAACAACA CTATCCCACC GAATATGATT 
AACACGTTTA AAAAGAATCA ACCGAAGAAT TATGAGTACT ACGATTCATT ACTTCAAGGT 
ATTATTCAAG AGTTGAACAA ATTGAATGTT CCTGAGAAAT GGAATTCTCA AACTTGGGCG 
AAATACATGA AGGTATATCT CGAAGCTCGC GGAATCGTTG ATCTGATGCC TGCGATGGTT 
GCATTCAAAT ACATGGAAAA AGCTATTGGG AAATTGAATG AAAGTCATTC AGAGACAGTC 
GAATACAGCA CCTTCATCAA AGACCATGAT ACTTTGAAAC AAACAATTCA ATCCGTTGAA 
CCAGAAATTG TTCTACGTCT CAAGAATACA CTCACCAATC AGTTCCACGT GGCTCCTGAA 
TCTCGAGTTA TTATTTTCGT AACACAAAGA AGCACCGCTC AACGTGTCAG TGATTTCTTG 
AACGAATCTA AAGTTTTAGA TCAATTTGGT AATTATGGTG AACAAATGGT TGGATATGTT 
CTCGGAACAA ACAAACAGGG CGCTGTACAG CAGACTTCAC AAGAACAACA ATTGACACTT 
GATAAATTCA ACAATGGAAG ACTTAAAGTA ATTGTGGCAA CGTCAGTTGT TGAAGAAGGT 
CTCGATGTTA CTGCATGTAA TCTTATTATC AAGTACAACT GTTCATCAGG ATCAGCCATT 
CAACTTGTTC AACAAAGAGG TCGTGCACGT GCCAAAAACT CTCGTTCTGT GCTTTTATCA 
GTGAAGAGTA GTATCAATGA AACCGAGACT AACGCGTTAA TCTCTGAAAA GTATATGAGA 
CTTTGTGTGA AGAAGATCAC AGAGAATGGC GAGAAACAAT TGGCTGCAGA AGTGAAACGA 
GTCGCTGAAC TAAATGCGGC TGAAAGGAAA CGAAATCTGG AAGAGCAACT CAATTTGAGA 
TTACGACATG AGAATAAGAT TTATAAATTG ATGTGCAGCA ATTGTTCAAA AGAGTTCTGC 
AAGAGTATTT ATATCAAAAA AGTGTTCTCG AATTACATGG TGTTCGATCC AAGTGTATGG 
AGATTTTTGC ATGTTGAATC GAAGCGAAAG GTGAGTAAGT ACTTGAGTGA GGACAATCAA 
CCACTCAGTG ATATCAAATG CTTCCATTGC AAGTTGGATG TTGGAAGAGC TTACAAAATA 
AGAGGAACCT ATTTGCCGCA ACTCAGTGTC AAGGCGTTGA CATTTGTTCA AGAATCAGAC 
TATTCCTCGA TGACAAAAGC AAAATGGTCG GATGTGGAAC AGGATCTGTT TTATATTTCA 
GAAGCAATCG AGGATGACTT TAGAATCATG TTGAATGCGC TCTCCGATAC TGAAGAAAAT 
ATCGAAAAGA AGATTGTTCT TGATTTGGAC TCACGACAAC ACAATAAGCA ACTCGAGATG 
AAGAGATTCC ATATTCAACA AGAACCACCA ACGAAGGGAG TTGCACCAGA AGCTCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492161.3
CDS	13..3372
Translation	MQPTAIRLEDYDKSKLRLPFESPYFPAYFRLLKWKFLDVCVESTRNNDIGYFKLFESLFPPGKLEEIARMIIDEPTPVSHDPDMIKIRNADLDVKIRKQAETYVTLRHAHQQKVQRRRFSECFLNTVLFDEKGLRIADEVMFNYDKELYGYSHWEDLPDGWLTAETFKNKFYDEEEVTNNPFGYQKLDRVAGAARGMIIMKHLKSNPRCVSETTILAFEVFNKGNHQLSTDLVEDLLTEGPAFELKIENGEEKKYAVKKWSLHKTLTMFLAIIGFKSNDKKEKNEHEEWYYGFIDAMKNDPANRAALYFLDKNWPEELEEREKERDRIRLTLLKSQRTNEEAVGEDVCTTIRPQPKDSGYNPDAVVTELVLRTYQEELVQPALEGKNCVIVAPTGSGKTEVAIYAALKHIEERTSQGKPSRVVLLVPKIPLVGQQKDRFLKYCNGMYEVNGFHGSESSVSGTGRRDEVIATHVSVMTPQILINMLQSVRQNERLYVSDFSMMIFDEVHKAAKNHPYVLINQMVQEWKYEKPQIIGLTASLSVKVDGQKDENQMLNDIYNMLALINAPHLSTITRQSSIDELNEHVGKPDDSVELCLPAKENILRDYIERYLNHAHGKFLEELASMSKSTGRNNTIPPNMINTFKKNQPKNYEYYDSLLQGIIQELNKLNVPEKWNSQTWAKYMKVYLEARGIVDLMPAMVAFKYMEKAIGKLNESHSETVEYSTFIKDHDTLKQTIQSVEPEIVLRLKNTLTNQFHVAPESRVIIFVTQRSTAQRVSDFLNESKVLDQFGNYGEQMVGYVLGTNKQGAVQQTSQEQQLTLDKFNNGRLKVIVATSVVEEGLDVTACNLIIKYNCSSGSAIQLVQQRGRARAKNSRSVLLSVKSSINETETNALISEKYMRLCVKKITENGEKQLAAEVKRVAELNAAERKRNLEEQLNLRLRHENKIYKLMCSNCSKEFCKSIYIKKVFSNYMVFDPSVWRFLHVESKRKVSKYLSEDNQPLSDIKCFHCKLDVGRAYKIRGTYLPQLSVKALTFVQESDYSSMTKAKWSDVEQDLFYISEAIEDDFRIMLNALSDTEENIEKKIVLDLDSRQHNKQLEMKRFHIQQEPPTKGVAPEAQ

Target ORF information:

RefSeq Version	NM_059760.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Dicer Related Helicase (drh-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059760.6

ORF Insert Sequence:

ATGCAACCAA CTGCAATACG TTTAGAAGAT TACGACAAAT CGAAGCTTCG CCTTCCGTTT 
GAGTCTCCAT ATTTTCCAGC GTATTTTCGT CTTCTCAAAT GGAAATTCCT TGATGTATGC 
GTGGAGAGTA CACGAAACAA CGATATCGGA TATTTCAAAC TATTTGAATC GCTGTTCCCG 
CCAGGAAAAT TGGAAGAAAT TGCCAGAATG ATCATTGACG AGCCAACTCC AGTTTCTCAT 
GACCCGGACA TGATTAAAAT CAGAAATGCC GATCTCGACG TTAAAATCAG AAAACAGGCA 
GAAACATACG TTACATTGCG TCACGCACAT CAGCAGAAAG TTCAAAGGAG AAGATTCTCG 
GAATGTTTTT TGAACACTGT TTTGTTCGAT GAGAAGGGAC TCAGAATTGC TGACGAAGTG 
ATGTTTAACT ACGATAAAGA ACTTTATGGA TACTCACACT GGGAAGATCT TCCTGATGGC 
TGGTTAACTG CTGAGACTTT CAAAAATAAA TTCTACGATG AAGAGGAAGT TACCAATAAT 
CCATTTGGAT ATCAGAAGCT TGATAGAGTG GCTGGAGCTG CACGTGGAAT GATTATTATG 
AAGCATCTGA AATCGAATCC ACGTTGTGTT AGTGAAACTA CAATTCTCGC TTTTGAAGTA 
TTTAACAAAG GTAATCATCA ACTATCAACT GATTTGGTCG AAGATTTGCT AACCGAAGGA 
CCAGCGTTCG AGCTGAAAAT CGAGAATGGA GAAGAGAAAA AATATGCTGT GAAGAAATGG 
AGTCTTCATA AGACTCTCAC CATGTTTCTC GCAATCATCG GATTCAAATC GAATGACAAA 
AAAGAGAAAA ATGAACACGA AGAGTGGTAT TACGGATTTA TTGATGCAAT GAAGAATGAT 
CCAGCTAATC GAGCGGCTCT CTACTTTTTG GATAAGAACT GGCCAGAAGA GCTTGAAGAA 
CGAGAGAAAG AACGTGACCG TATCCGCCTT ACCCTACTGA AAAGTCAGCG GACTAACGAG 
GAAGCTGTCG GCGAGGATGT TTGCACGACA ATACGTCCAC AACCGAAAGA CAGCGGCTAC 
AATCCAGATG CGGTGGTTAC TGAACTTGTA TTGCGTACTT ATCAAGAAGA ACTTGTCCAA 
CCGGCTCTCG AAGGAAAGAA TTGTGTTATT GTTGCTCCGA CTGGTTCTGG TAAAACAGAA 
GTGGCAATTT ATGCGGCTCT CAAACATATT GAAGAACGAA CATCCCAAGG AAAGCCTTCC 
AGAGTTGTGC TCCTTGTGCC GAAAATCCCG CTTGTCGGTC AACAAAAAGA CAGATTCCTG 
AAGTATTGTA ACGGAATGTA CGAAGTTAAT GGTTTTCATG GATCCGAGAG CAGTGTCAGT 
GGAACAGGAC GTCGTGATGA AGTAATTGCT ACTCATGTAT CAGTTATGAC TCCTCAAATT 
CTGATCAACA TGCTTCAATC GGTACGTCAA AATGAGCGTT TGTATGTGAG TGATTTCTCA 
ATGATGATCT TCGATGAAGT ACATAAAGCT GCAAAAAATC ATCCATATGT GTTGATTAAT 
CAAATGGTTC AAGAATGGAA GTATGAAAAA CCACAAATCA TTGGTCTGAC AGCTTCATTG 
AGTGTCAAAG TTGATGGACA AAAAGACGAG AATCAGATGC TTAATGATAT TTATAATATG 
CTCGCATTGA TCAACGCTCC ACATTTGAGT ACAATCACTC GCCAATCGAG CATAGATGAG 
CTCAATGAGC ATGTTGGAAA ACCAGATGAT TCTGTCGAAC TATGCTTGCC TGCCAAAGAA 
AATATCCTTC GAGATTACAT CGAACGATAC CTTAACCACG CCCATGGCAA ATTTCTCGAA 
GAATTAGCAA GCATGTCTAA ATCAACTGGC CGCAACAACA CTATCCCACC GAATATGATT 
AACACGTTTA AAAAGAATCA ACCGAAGAAT TATGAGTACT ACGATTCATT ACTTCAAGGT 
ATTATTCAAG AGTTGAACAA ATTGAATGTT CCTGAGAAAT GGAATTCTCA AACTTGGGCG 
AAATACATGA AGGTATATCT CGAAGCTCGC GGAATCGTTG ATCTGATGCC TGCGATGGTT 
GCATTCAAAT ACATGGAAAA AGCTATTGGG AAATTGAATG AAAGTCATTC AGAGACAGTC 
GAATACAGCA CCTTCATCAA AGACCATGAT ACTTTGAAAC AAACAATTCA ATCCGTTGAA 
CCAGAAATTG TTCTACGTCT CAAGAATACA CTCACCAATC AGTTCCACGT GGCTCCTGAA 
TCTCGAGTTA TTATTTTCGT AACACAAAGA AGCACCGCTC AACGTGTCAG TGATTTCTTG 
AACGAATCTA AAGTTTTAGA TCAATTTGGT AATTATGGTG AACAAATGGT TGGATATGTT 
CTCGGAACAA ACAAACAGGG CGCTGTACAG CAGACTTCAC AAGAACAACA ATTGACACTT 
GATAAATTCA ACAATGGAAG ACTTAAAGTA ATTGTGGCAA CGTCAGTTGT TGAAGAAGGT 
CTCGATGTTA CTGCATGTAA TCTTATTATC AAGTACAACT GTTCATCAGG ATCAGCCATT 
CAACTTGTTC AACAAAGAGG TCGTGCACGT GCCAAAAACT CTCGTTCTGT GCTTTTATCA 
GTGAAGAGTA GTATCAATGA AACCGAGACT AACGCGTTAA TCTCTGAAAA GTATATGAGA 
CTTTGTGTGA AGAAGATCAC AGAGAATGGC GAGAAACAAT TGGCTGCAGA AGTGAAACGA 
GTCGCTGAAC TAAATGCGGC TGAAAGGAAA CGAAATCTGG AAGAGCAACT CAATTTGAGA 
TTACGACATG AGAATAAGAT TTATAAATTG ATGTGCAGCA ATTGTTCAAA AGAGTTCTGC 
AAGAGTATTT ATATCAAAAA AGTGTTCTCG AATTACATGG TGTTCGATCC AAGTGTATGG 
AGATTTTTGC ATGTTGAATC GAAGCGAAAG GTGAGTAAGT ACTTGAGTGA GGACAATCAA 
CCACTCAGTG ATATCAAATG CTTCCATTGC AAGTTGGATG TTGGAAGAGC TTACAAAATA 
AGAGGAACCT ATTTGCCGCA ACTCAGTGTC AAGGCGTTGA CATTTGTTCA AGAATCAGAC 
TATTCCTCGA TGACAAAAGC AAAATGGTCG GATGTGGAAC AGGATCTGTT TTATATTTCA 
GAAGCAATCG AGGATGACTT TAGAATCATG TTGAATGCGC TCTCCGATAC TGAAGAAAAT 
ATCGAAAAGA AGATTGTTCT TGATTTGGAC TCACGACAAC ACAATAAGCA ACTCGAGATG 
AAGAGATTCC ATATTCAACA AGAACCACCA ACGAAGGGAG TTGCACCAGA AGCTCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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drh-3 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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