chs-1 cDNA ORF clone, Caenorhabditis elegans

The following chs-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chs-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OCa335291 NM_059712.5
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Caenorhabditis elegans Chitin synthase chs-1 (chs-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
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OCa335291 NM_059712.4 Caenorhabditis elegans Chitin synthase chs-1 (chs-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa335291
    Clone ID Related Accession (Same CDS sequence) NM_059712.4 , NM_059712.5
    Accession Version NM_059712.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Chitin synthase chs-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_059712.3. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAACGATG GGGAAAACTA TTGGAATGCG TTTCGATCGC ACAAAAGATC TGCCACTGAT 
    GGACCGACCC TTTCGCCATG GATGGTCACA GTTTTACAGG CGACAAAGCT ACTTCTTTTC
    GCACTTTGTA ATATAGTATT AACACTTGGA TCAGTTTTTT CGAAACTCAT CGTATTGATC
    ATGGCTACTA ACATCGTCCC TCGAGCTCAT CTCATTGGGA AATTCGCCAG GAAATGCACG
    AAAGCCGCCG TTCGACGTAC ATCTACAACA ACTGCGGGAA TATATCTGTC GCTACTATTG
    ATTCAGTGTT TCCCTGATAC TATCAATCTG ATACGTTCTG GCATAGATAT GTGGAAAGGA
    CAATGTGGTC AACTCGTCAA GTCAGTGGTT GTTCTGGAAT CTCTTCGAGC AATCGGACTC
    GCCGTTCTGA GCTTCCACGT TTTCCCTCAA TTGGATCTCG CCAGATGCCT TGTTCTCAGC
    GCATGCTTCC CACTTGTGGC TGTGTTGCAA AGATCTTTGG TAGCAATGGT TTCTGCTGCT
    AGAACAGGAA GATCATTCCG AAATCGTCTC GGCCGATGCT TCGTTGCAAT TCCACATGTC
    ATTATGTTCT TGGTTCTCAT GAGTAGCTGT TATGTTTGGG CGCTATTTGA TAACAAGTTC
    ACAGCTATAA TTGCACTTCC GATTGGAGTA ATTTGCACTT CAGCTGGCTT TTGGGAATCT
    TGGATTGACA CAACGCATTC TGGTACTTCA TTTGATGAGT TATACAGGCT CAAATATGCT
    GTACGGAAGA TGAACACCAC CACGAAACTT ATTGTTTCCC TCATGCGCAT CGTTTGTACA
    GTTTCAGTTC TGGTATCTGC TGTCTATATT AACGATCACA AGAAGCTTAA TTCATCTCAT
    TTTGTCAAGG CATTCTTTAG TTTCAGTACA CGGCAACCCC ACACGAGGCT TCTTCTACTA
    GCCACCGGAA TCATTGTTCT CCATTTTGTC ATGCGAGGGA TTTCACGCTT CTTGGCTGCT
    CTTGATCTGC ATCCGTTCTC TTTCGTGCAC CCACTCTCCA TTGCTCCTCT CATCGCTTAT
    GGATACGTCC GTTATGCTTG CCAATCACCC ACATGTTCAA TAGCTCGACG ATTGGCTAGA
    TTTGGTCTTC ATTGGGTGTG TGATCAATGG TTCCAAAGCG CACGAGGAAT CGCATCTCCG
    GACTTTTACA TTTGCCTTAT TTGGTTGTTG GTAGGATGCT ATAGAGGATG GAGATTGGTT
    AGACAACGAT ACTTTGATAC GAACGAAGAA ATCATCTCTT CAATGCCTCC TGTCTGTAAT
    GGCCTCTGCA TCGAGCAATC TCTTGTGGTA TTCCAACATT CACTGAATCG GCAAGAAAAA
    ACTATGCTTA CCGAAGAAGA AGACATATCG GATGAGAATG ATGAGCTACG AATTCGAAAC
    GATGAAGTTG ACAGAGTGTC TACGGTTTAT GGATGTGCAA CCATGTGGCA TGAAACAGAA
    ACCGAGATGC GACAAGTACT TCGTAGTATT CTGAAGCTTG ATGTGGATCA TGCGACACGT
    ATGAACAACA AGAAAGCTAA TGAACTTCGA TACCGATTGG AAGGTCACAT TTTCTTCGAT
    GACGCATGGG AAGATGTCGA AGAAGATGGA ATAGAGAAAA GGCAGCCAAA TGAATACTTC
    AACATGTTCT TTGACTTGCT CAATGAAATG ACTGGTGAAA GATTAAATGA AGAAGGAAAG
    ATGGAAACAA GAATTCTCGT CAATACACCT TACGGTGGTC GTCTTGTTGT AAAACTTCCA
    TCAGGAACTT TACTCTTCGT ACATTTGAAG GATAAAAAGA TGATTCGACA CAAAAAGCGA
    TGGAGCCAAG TTATGTACAT GTATTACCTT CTTGGTCATC GTATCATGGA TTGCCCACTT
    TCTATTGAAG ATAGACAACA GATGGCTGAC AACACGTTTA TTCTGGCTAT TGATGGAGAT
    TCTAAGTTCG AACCTGACGC ACTTCTTCGT CTTCTCCACT TGATGAATGC GAAAAGTGAT
    ATTGGATGTG CTTGTGGAAG AATTCACCCA ATTGGAAATG GTATAATGGT TTGGTATCAG
    AAGTTTGAGT ACGCTATTGC TCATTGGTTT CAAAAGGCTG CTGAACATGT TTTCGGTTGT
    GTCCTTTGTG CGCCTGGATG TTTCTCATTG TTCCGTGCAT CTGCATTGAT GGATGACAAT
    ATCATGCACA AGTATACTAA GACCGCTTCC GAACCACGTC ACTACGTCCA ATATGATCAA
    GGAGAAGACA GATGGTTAAG TACATTGCTT TTGAAACAAG GATATCGTAT CGAATACGCC
    GCTGCGTCTG ATGCTGAAAC ATACGCTCCG GAAGGATTCG AAGAATTTTT CAATCAACGT
    AGACGCTGGA CTCCTTCGTC GATTGCTAAT ACAGTAGATC TTCTGATGGA TTACAAGAGA
    GCTTCAGAAA ATAATGACGC CATTTCATAT GCCTACATCG CCTATCAATT CCTTGTGATC
    TTCTTCTCAA TGCTCGGACC TGCCATTATA TTCACTATGC TCGTATTCGC CCAAGTTGCT
    GCTTTTGAAC TTCGAGGATC TGATGTGATG CTCTATAATG GAATTCCGAT AGGATTTTTC
    ATTGTTCTTT GTTTCACAAC AGAGTCGAAT ATTCAACTTA TCTATGCCAA GTACATGTCG
    ATTGCGTACG CGTTCGTAAT GTTGGCTGTG TTAGTCGCTA CCAGCAGTCA AATTGTTCTC
    GAGACTGTTC TCGCTCCTAC ATCTCTCTTC ATTGTCACAA TGGTCGGAAT CTTCTTCTTT
    GCTGCATGTC TTCATCCAAA AGAATTCACG AATATTATCC ATGGTGTCGT ATTCTTCCTC
    ATGATTCCAT CTACATATGT GTTCCTCACT TTATATTCGC TCATCAATCT CAACGTTATC
    ACGTGGGGAA CTCGTGAAGC TGTCGCTAAG GCAACGGGAC AAAAGACGAA AAAAGCGCCT
    ATGGAACAAT TTATAGACAG AGTGATTGAT ATTGTGAAAA AGGGATTCAG ATTAATCAGT
    TGTCGGGAGA AGAAGGAACA TGAAGAGAGA CGAGAGAAAA TGGAAAAGAA AATGCAGAGA
    ATGGAGCTAG CCTTGAGAAG TATTGAGAGT GGTGCCGACG TGAAGAAAAT TCTCGATGCA
    ACAGAGGAGA AGGAGAAACG TGAAGAAGAA ACTCAAACTG CAGATTTTCC GATTGAAGAG
    AACGTAGAGA AGACTCAAAA AGAGATTCAG AAGGCAAACC GTTATGTGTG GATGACAAGT
    CATAGCTTGA AAGTTTGTGA ACGAGGAAAA CTGAAAAGTG CGGAAAAGGT TTTCTGGAAC
    GAGCTCATCA ATGCATATCT GAAACCGATC AAGACGACGC CAGCTGAAAT GAAAGCCGTC
    GCCGAAGGAT TGGCTTCTCT ACGAAATCAG ATTGCTTTCA CTATTCTTCT CGTTAATTCT
    CTTCTTGCTC TTGCCATCTT TTTGATTCAG AAACACAAAA ATGTGCTCAG CATCAAGTTC
    TCGCCAATCA AAAACTTCCG ATGGACGAAA ATGAATGAGA TGACTGGACA ATACGAGGAA
    ACCGATGAAC CATTAAAAAT AGATCCACTT GGAATGGGAA TTGTTGTTTT CCTTCTAATT
    ATTCTTTTTG TTCAAACTCT CGGAATGCTT CTCCATCGAC TTAACACTAT GATAGGAGCT
    TTCCAAGAAG TGAAAAATTT GTACGAATAC GGAGTTTCAC CAGTGATAAA TACAAAGAAT
    GATGATGAGA GGATTATGAA TAATGCTCGT CTAATGATTA ATTCTCTCGG TGTCAGTACT
    GGACACGCTG CCGACGGCTA TACAAGGCAT CGCGGAGAGG AATCCGATAC CGGCAATGTT
    CTCTACAAAC TTCAAAAGGC TCGTCTCGCA AAGCGAATGC AAAGAAGTGC ACTTTCCACA
    ACTGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_492113.2
    CDS1..3969
    Translation

    Target ORF information:

    RefSeq Version NM_059712.4
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Chitin synthase chs-1 (chs-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_059712.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAACGATG GGGAAAACTA TTGGAATGCG TTTCGATCGC ACAAAAGATC TGCCACTGAT 
    GGACCGACCC TTTCGCCATG GATGGTCACA GTTTTACAGG CGACAAAGCT ACTTCTTTTC
    GCACTTTGTA ATATAGTATT AACACTTGGA TCAGTTTTTT CGAAACTCAT CGTATTGATC
    ATGGCTACTA ACATCGTCCC TCGAGCTCAT CTCATTGGGA AATTCGCCAG GAAATGCACG
    AAAGCCGCCG TTCGACGTAC ATCTACAACA ACTGCGGGAA TATATCTGTC GCTACTATTG
    ATTCAGTGTT TCCCTGATAC TATCAATCTG ATACGTTCTG GCATAGATAT GTGGAAAGGA
    CAATGTGGTC AACTCGTCAA GTCAGTGGTT GTTCTGGAAT CTCTTCGAGC AATCGGACTC
    GCCGTTCTGA GCTTCCACGT TTTCCCTCAA TTGGATCTCG CCAGATGCCT TGTTCTCAGC
    GCATGCTTCC CACTTGTGGC TGTGTTGCAA AGATCTTTGG TAGCAATGGT TTCTGCTGCT
    AGAACAGGAA GATCATTCCG AAATCGTCTC GGCCGATGCT TCGTTGCAAT TCCACATGTC
    ATTATGTTCT TGGTTCTCAT GAGTAGCTGT TATGTTTGGG CGCTATTTGA TAACAAGTTC
    ACAGCTATAA TTGCACTTCC GATTGGAGTA ATTTGCACTT CAGCTGGCTT TTGGGAATCT
    TGGATTGACA CAACGCATTC TGGTACTTCA TTTGATGAGT TATACAGGCT CAAATATGCT
    GTACGGAAGA TGAACACCAC CACGAAACTT ATTGTTTCCC TCATGCGCAT CGTTTGTACA
    GTTTCAGTTC TGGTATCTGC TGTCTATATT AACGATCACA AGAAGCTTAA TTCATCTCAT
    TTTGTCAAGG CATTCTTTAG TTTCAGTACA CGGCAACCCC ACACGAGGCT TCTTCTACTA
    GCCACCGGAA TCATTGTTCT CCATTTTGTC ATGCGAGGGA TTTCACGCTT CTTGGCTGCT
    CTTGATCTGC ATCCGTTCTC TTTCGTGCAC CCACTCTCCA TTGCTCCTCT CATCGCTTAT
    GGATACGTCC GTTATGCTTG CCAATCACCC ACATGTTCAA TAGCTCGACG ATTGGCTAGA
    TTTGGTCTTC ATTGGGTGTG TGATCAATGG TTCCAAAGCG CACGAGGAAT CGCATCTCCG
    GACTTTTACA TTTGCCTTAT TTGGTTGTTG GTAGGATGCT ATAGAGGATG GAGATTGGTT
    AGACAACGAT ACTTTGATAC GAACGAAGAA ATCATCTCTT CAATGCCTCC TGTCTGTAAT
    GGCCTCTGCA TCGAGCAATC TCTTGTGGTA TTCCAACATT CACTGAATCG GCAAGAAAAA
    ACTATGCTTA CCGAAGAAGA AGACATATCG GATGAGAATG ATGAGCTACG AATTCGAAAC
    GATGAAGTTG ACAGAGTGTC TACGGTTTAT GGATGTGCAA CCATGTGGCA TGAAACAGAA
    ACCGAGATGC GACAAGTACT TCGTAGTATT CTGAAGCTTG ATGTGGATCA TGCGACACGT
    ATGAACAACA AGAAAGCTAA TGAACTTCGA TACCGATTGG AAGGTCACAT TTTCTTCGAT
    GACGCATGGG AAGATGTCGA AGAAGATGGA ATAGAGAAAA GGCAGCCAAA TGAATACTTC
    AACATGTTCT TTGACTTGCT CAATGAAATG ACTGGTGAAA GATTAAATGA AGAAGGAAAG
    ATGGAAACAA GAATTCTCGT CAATACACCT TACGGTGGTC GTCTTGTTGT AAAACTTCCA
    TCAGGAACTT TACTCTTCGT ACATTTGAAG GATAAAAAGA TGATTCGACA CAAAAAGCGA
    TGGAGCCAAG TTATGTACAT GTATTACCTT CTTGGTCATC GTATCATGGA TTGCCCACTT
    TCTATTGAAG ATAGACAACA GATGGCTGAC AACACGTTTA TTCTGGCTAT TGATGGAGAT
    TCTAAGTTCG AACCTGACGC ACTTCTTCGT CTTCTCCACT TGATGAATGC GAAAAGTGAT
    ATTGGATGTG CTTGTGGAAG AATTCACCCA ATTGGAAATG GTATAATGGT TTGGTATCAG
    AAGTTTGAGT ACGCTATTGC TCATTGGTTT CAAAAGGCTG CTGAACATGT TTTCGGTTGT
    GTCCTTTGTG CGCCTGGATG TTTCTCATTG TTCCGTGCAT CTGCATTGAT GGATGACAAT
    ATCATGCACA AGTATACTAA GACCGCTTCC GAACCACGTC ACTACGTCCA ATATGATCAA
    GGAGAAGACA GATGGTTAAG TACATTGCTT TTGAAACAAG GATATCGTAT CGAATACGCC
    GCTGCGTCTG ATGCTGAAAC ATACGCTCCG GAAGGATTCG AAGAATTTTT CAATCAACGT
    AGACGCTGGA CTCCTTCGTC GATTGCTAAT ACAGTAGATC TTCTGATGGA TTACAAGAGA
    GCTTCAGAAA ATAATGACGC CATTTCATAT GCCTACATCG CCTATCAATT CCTTGTGATC
    TTCTTCTCAA TGCTCGGACC TGCCATTATA TTCACTATGC TCGTATTCGC CCAAGTTGCT
    GCTTTTGAAC TTCGAGGATC TGATGTGATG CTCTATAATG GAATTCCGAT AGGATTTTTC
    ATTGTTCTTT GTTTCACAAC AGAGTCGAAT ATTCAACTTA TCTATGCCAA GTACATGTCG
    ATTGCGTACG CGTTCGTAAT GTTGGCTGTG TTAGTCGCTA CCAGCAGTCA AATTGTTCTC
    GAGACTGTTC TCGCTCCTAC ATCTCTCTTC ATTGTCACAA TGGTCGGAAT CTTCTTCTTT
    GCTGCATGTC TTCATCCAAA AGAATTCACG AATATTATCC ATGGTGTCGT ATTCTTCCTC
    ATGATTCCAT CTACATATGT GTTCCTCACT TTATATTCGC TCATCAATCT CAACGTTATC
    ACGTGGGGAA CTCGTGAAGC TGTCGCTAAG GCAACGGGAC AAAAGACGAA AAAAGCGCCT
    ATGGAACAAT TTATAGACAG AGTGATTGAT ATTGTGAAAA AGGGATTCAG ATTAATCAGT
    TGTCGGGAGA AGAAGGAACA TGAAGAGAGA CGAGAGAAAA TGGAAAAGAA AATGCAGAGA
    ATGGAGCTAG CCTTGAGAAG TATTGAGAGT GGTGCCGACG TGAAGAAAAT TCTCGATGCA
    ACAGAGGAGA AGGAGAAACG TGAAGAAGAA ACTCAAACTG CAGATTTTCC GATTGAAGAG
    AACGTAGAGA AGACTCAAAA AGAGATTCAG AAGGCAAACC GTTATGTGTG GATGACAAGT
    CATAGCTTGA AAGTTTGTGA ACGAGGAAAA CTGAAAAGTG CGGAAAAGGT TTTCTGGAAC
    GAGCTCATCA ATGCATATCT GAAACCGATC AAGACGACGC CAGCTGAAAT GAAAGCCGTC
    GCCGAAGGAT TGGCTTCTCT ACGAAATCAG ATTGCTTTCA CTATTCTTCT CGTTAATTCT
    CTTCTTGCTC TTGCCATCTT TTTGATTCAG AAACACAAAA ATGTGCTCAG CATCAAGTTC
    TCGCCAATCA AAAACTTCCG ATGGACGAAA ATGAATGAGA TGACTGGACA ATACGAGGAA
    ACCGATGAAC CATTAAAAAT AGATCCACTT GGAATGGGAA TTGTTGTTTT CCTTCTAATT
    ATTCTTTTTG TTCAAACTCT CGGAATGCTT CTCCATCGAC TTAACACTAT GATAGGAGCT
    TTCCAAGAAG TGAAAAATTT GTACGAATAC GGAGTTTCAC CAGTGATAAA TACAAAGAAT
    GATGATGAGA GGATTATGAA TAATGCTCGT CTAATGATTA ATTCTCTCGG TGTCAGTACT
    GGACACGCTG CCGACGGCTA TACAAGGCAT CGCGGAGAGG AATCCGATAC CGGCAATGTT
    CTCTACAAAC TTCAAAAGGC TCGTCTCGCA AAGCGAATGC AAAGAAGTGC ACTTTCCACA
    ACTGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa335291
    Clone ID Related Accession (Same CDS sequence) NM_059712.4 , NM_059712.5
    Accession Version NM_059712.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Chitin synthase chs-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059712.4.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAACGATG GGGAAAACTA TTGGAATGCG TTTCGATCGC ACAAAAGATC TGCCACTGAT 
    GGACCGACCC TTTCGCCATG GATGGTCACA GTTTTACAGG CGACAAAGCT ACTTCTTTTC
    GCACTTTGTA ATATAGTATT AACACTTGGA TCAGTTTTTT CGAAACTCAT CGTATTGATC
    ATGGCTACTA ACATCGTCCC TCGAGCTCAT CTCATTGGGA AATTCGCCAG GAAATGCACG
    AAAGCCGCCG TTCGACGTAC ATCTACAACA ACTGCGGGAA TATATCTGTC GCTACTATTG
    ATTCAGTGTT TCCCTGATAC TATCAATCTG ATACGTTCTG GCATAGATAT GTGGAAAGGA
    CAATGTGGTC AACTCGTCAA GTCAGTGGTT GTTCTGGAAT CTCTTCGAGC AATCGGACTC
    GCCGTTCTGA GCTTCCACGT TTTCCCTCAA TTGGATCTCG CCAGATGCCT TGTTCTCAGC
    GCATGCTTCC CACTTGTGGC TGTGTTGCAA AGATCTTTGG TAGCAATGGT TTCTGCTGCT
    AGAACAGGAA GATCATTCCG AAATCGTCTC GGCCGATGCT TCGTTGCAAT TCCACATGTC
    ATTATGTTCT TGGTTCTCAT GAGTAGCTGT TATGTTTGGG CGCTATTTGA TAACAAGTTC
    ACAGCTATAA TTGCACTTCC GATTGGAGTA ATTTGCACTT CAGCTGGCTT TTGGGAATCT
    TGGATTGACA CAACGCATTC TGGTACTTCA TTTGATGAGT TATACAGGCT CAAATATGCT
    GTACGGAAGA TGAACACCAC CACGAAACTT ATTGTTTCCC TCATGCGCAT CGTTTGTACA
    GTTTCAGTTC TGGTATCTGC TGTCTATATT AACGATCACA AGAAGCTTAA TTCATCTCAT
    TTTGTCAAGG CATTCTTTAG TTTCAGTACA CGGCAACCCC ACACGAGGCT TCTTCTACTA
    GCCACCGGAA TCATTGTTCT CCATTTTGTC ATGCGAGGGA TTTCACGCTT CTTGGCTGCT
    CTTGATCTGC ATCCGTTCTC TTTCGTGCAC CCACTCTCCA TTGCTCCTCT CATCGCTTAT
    GGATACGTCC GTTATGCTTG CCAATCACCC ACATGTTCAA TAGCTCGACG ATTGGCTAGA
    TTTGGTCTTC ATTGGGTGTG TGATCAATGG TTCCAAAGCG CACGAGGAAT CGCATCTCCG
    GACTTTTACA TTTGCCTTAT TTGGTTGTTG GTAGGATGCT ATAGAGGATG GAGATTGGTT
    AGACAACGAT ACTTTGATAC GAACGAAGAA ATCATCTCTT CAATGCCTCC TGTCTGTAAT
    GGCCTCTGCA TCGAGCAATC TCTTGTGGTA TTCCAACATT CACTGAATCG GCAAGAAAAA
    ACTATGCTTA CCGAAGAAGA AGACATATCG GATGAGAATG ATGAGCTACG AATTCGAAAC
    GATGAAGTTG ACAGAGTGTC TACGGTTTAT GGATGTGCAA CCATGTGGCA TGAAACAGAA
    ACCGAGATGC GACAAGTACT TCGTAGTATT CTGAAGCTTG ATGTGGATCA TGCGACACGT
    ATGAACAACA AGAAAGCTAA TGAACTTCGA TACCGATTGG AAGGTCACAT TTTCTTCGAT
    GACGCATGGG AAGATGTCGA AGAAGATGGA ATAGAGAAAA GGCAGCCAAA TGAATACTTC
    AACATGTTCT TTGACTTGCT CAATGAAATG ACTGGTGAAA GATTAAATGA AGAAGGAAAG
    ATGGAAACAA GAATTCTCGT CAATACACCT TACGGTGGTC GTCTTGTTGT AAAACTTCCA
    TCAGGAACTT TACTCTTCGT ACATTTGAAG GATAAAAAGA TGATTCGACA CAAAAAGCGA
    TGGAGCCAAG TTATGTACAT GTATTACCTT CTTGGTCATC GTATCATGGA TTGCCCACTT
    TCTATTGAAG ATAGACAACA GATGGCTGAC AACACGTTTA TTCTGGCTAT TGATGGAGAT
    TCTAAGTTCG AACCTGACGC ACTTCTTCGT CTTCTCCACT TGATGAATGC GAAAAGTGAT
    ATTGGATGTG CTTGTGGAAG AATTCACCCA ATTGGAAATG GTATAATGGT TTGGTATCAG
    AAGTTTGAGT ACGCTATTGC TCATTGGTTT CAAAAGGCTG CTGAACATGT TTTCGGTTGT
    GTCCTTTGTG CGCCTGGATG TTTCTCATTG TTCCGTGCAT CTGCATTGAT GGATGACAAT
    ATCATGCACA AGTATACTAA GACCGCTTCC GAACCACGTC ACTACGTCCA ATATGATCAA
    GGAGAAGACA GATGGTTAAG TACATTGCTT TTGAAACAAG GATATCGTAT CGAATACGCC
    GCTGCGTCTG ATGCTGAAAC ATACGCTCCG GAAGGATTCG AAGAATTTTT CAATCAACGT
    AGACGCTGGA CTCCTTCGTC GATTGCTAAT ACAGTAGATC TTCTGATGGA TTACAAGAGA
    GCTTCAGAAA ATAATGACGC CATTTCATAT GCCTACATCG CCTATCAATT CCTTGTGATC
    TTCTTCTCAA TGCTCGGACC TGCCATTATA TTCACTATGC TCGTATTCGC CCAAGTTGCT
    GCTTTTGAAC TTCGAGGATC TGATGTGATG CTCTATAATG GAATTCCGAT AGGATTTTTC
    ATTGTTCTTT GTTTCACAAC AGAGTCGAAT ATTCAACTTA TCTATGCCAA GTACATGTCG
    ATTGCGTACG CGTTCGTAAT GTTGGCTGTG TTAGTCGCTA CCAGCAGTCA AATTGTTCTC
    GAGACTGTTC TCGCTCCTAC ATCTCTCTTC ATTGTCACAA TGGTCGGAAT CTTCTTCTTT
    GCTGCATGTC TTCATCCAAA AGAATTCACG AATATTATCC ATGGTGTCGT ATTCTTCCTC
    ATGATTCCAT CTACATATGT GTTCCTCACT TTATATTCGC TCATCAATCT CAACGTTATC
    ACGTGGGGAA CTCGTGAAGC TGTCGCTAAG GCAACGGGAC AAAAGACGAA AAAAGCGCCT
    ATGGAACAAT TTATAGACAG AGTGATTGAT ATTGTGAAAA AGGGATTCAG ATTAATCAGT
    TGTCGGGAGA AGAAGGAACA TGAAGAGAGA CGAGAGAAAA TGGAAAAGAA AATGCAGAGA
    ATGGAGCTAG CCTTGAGAAG TATTGAGAGT GGTGCCGACG TGAAGAAAAT TCTCGATGCA
    ACAGAGGAGA AGGAGAAACG TGAAGAAGAA ACTCAAACTG CAGATTTTCC GATTGAAGAG
    AACGTAGAGA AGACTCAAAA AGAGATTCAG AAGGCAAACC GTTATGTGTG GATGACAAGT
    CATAGCTTGA AAGTTTGTGA ACGAGGAAAA CTGAAAAGTG CGGAAAAGGT TTTCTGGAAC
    GAGCTCATCA ATGCATATCT GAAACCGATC AAGACGACGC CAGCTGAAAT GAAAGCCGTC
    GCCGAAGGAT TGGCTTCTCT ACGAAATCAG ATTGCTTTCA CTATTCTTCT CGTTAATTCT
    CTTCTTGCTC TTGCCATCTT TTTGATTCAG AAACACAAAA ATGTGCTCAG CATCAAGTTC
    TCGCCAATCA AAAACTTCCG ATGGACGAAA ATGAATGAGA TGACTGGACA ATACGAGGAA
    ACCGATGAAC CATTAAAAAT AGATCCACTT GGAATGGGAA TTGTTGTTTT CCTTCTAATT
    ATTCTTTTTG TTCAAACTCT CGGAATGCTT CTCCATCGAC TTAACACTAT GATAGGAGCT
    TTCCAAGAAG TGAAAAATTT GTACGAATAC GGAGTTTCAC CAGTGATAAA TACAAAGAAT
    GATGATGAGA GGATTATGAA TAATGCTCGT CTAATGATTA ATTCTCTCGG TGTCAGTACT
    GGACACGCTG CCGACGGCTA TACAAGGCAT CGCGGAGAGG AATCCGATAC CGGCAATGTT
    CTCTACAAAC TTCAAAAGGC TCGTCTCGCA AAGCGAATGC AAAGAAGTGC ACTTTCCACA
    ACTGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_492113.2
    CDS147..4115
    Translation

    Target ORF information:

    RefSeq Version NM_059712.5
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Chitin synthase chs-1 (chs-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_059712.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAACGATG GGGAAAACTA TTGGAATGCG TTTCGATCGC ACAAAAGATC TGCCACTGAT 
    GGACCGACCC TTTCGCCATG GATGGTCACA GTTTTACAGG CGACAAAGCT ACTTCTTTTC
    GCACTTTGTA ATATAGTATT AACACTTGGA TCAGTTTTTT CGAAACTCAT CGTATTGATC
    ATGGCTACTA ACATCGTCCC TCGAGCTCAT CTCATTGGGA AATTCGCCAG GAAATGCACG
    AAAGCCGCCG TTCGACGTAC ATCTACAACA ACTGCGGGAA TATATCTGTC GCTACTATTG
    ATTCAGTGTT TCCCTGATAC TATCAATCTG ATACGTTCTG GCATAGATAT GTGGAAAGGA
    CAATGTGGTC AACTCGTCAA GTCAGTGGTT GTTCTGGAAT CTCTTCGAGC AATCGGACTC
    GCCGTTCTGA GCTTCCACGT TTTCCCTCAA TTGGATCTCG CCAGATGCCT TGTTCTCAGC
    GCATGCTTCC CACTTGTGGC TGTGTTGCAA AGATCTTTGG TAGCAATGGT TTCTGCTGCT
    AGAACAGGAA GATCATTCCG AAATCGTCTC GGCCGATGCT TCGTTGCAAT TCCACATGTC
    ATTATGTTCT TGGTTCTCAT GAGTAGCTGT TATGTTTGGG CGCTATTTGA TAACAAGTTC
    ACAGCTATAA TTGCACTTCC GATTGGAGTA ATTTGCACTT CAGCTGGCTT TTGGGAATCT
    TGGATTGACA CAACGCATTC TGGTACTTCA TTTGATGAGT TATACAGGCT CAAATATGCT
    GTACGGAAGA TGAACACCAC CACGAAACTT ATTGTTTCCC TCATGCGCAT CGTTTGTACA
    GTTTCAGTTC TGGTATCTGC TGTCTATATT AACGATCACA AGAAGCTTAA TTCATCTCAT
    TTTGTCAAGG CATTCTTTAG TTTCAGTACA CGGCAACCCC ACACGAGGCT TCTTCTACTA
    GCCACCGGAA TCATTGTTCT CCATTTTGTC ATGCGAGGGA TTTCACGCTT CTTGGCTGCT
    CTTGATCTGC ATCCGTTCTC TTTCGTGCAC CCACTCTCCA TTGCTCCTCT CATCGCTTAT
    GGATACGTCC GTTATGCTTG CCAATCACCC ACATGTTCAA TAGCTCGACG ATTGGCTAGA
    TTTGGTCTTC ATTGGGTGTG TGATCAATGG TTCCAAAGCG CACGAGGAAT CGCATCTCCG
    GACTTTTACA TTTGCCTTAT TTGGTTGTTG GTAGGATGCT ATAGAGGATG GAGATTGGTT
    AGACAACGAT ACTTTGATAC GAACGAAGAA ATCATCTCTT CAATGCCTCC TGTCTGTAAT
    GGCCTCTGCA TCGAGCAATC TCTTGTGGTA TTCCAACATT CACTGAATCG GCAAGAAAAA
    ACTATGCTTA CCGAAGAAGA AGACATATCG GATGAGAATG ATGAGCTACG AATTCGAAAC
    GATGAAGTTG ACAGAGTGTC TACGGTTTAT GGATGTGCAA CCATGTGGCA TGAAACAGAA
    ACCGAGATGC GACAAGTACT TCGTAGTATT CTGAAGCTTG ATGTGGATCA TGCGACACGT
    ATGAACAACA AGAAAGCTAA TGAACTTCGA TACCGATTGG AAGGTCACAT TTTCTTCGAT
    GACGCATGGG AAGATGTCGA AGAAGATGGA ATAGAGAAAA GGCAGCCAAA TGAATACTTC
    AACATGTTCT TTGACTTGCT CAATGAAATG ACTGGTGAAA GATTAAATGA AGAAGGAAAG
    ATGGAAACAA GAATTCTCGT CAATACACCT TACGGTGGTC GTCTTGTTGT AAAACTTCCA
    TCAGGAACTT TACTCTTCGT ACATTTGAAG GATAAAAAGA TGATTCGACA CAAAAAGCGA
    TGGAGCCAAG TTATGTACAT GTATTACCTT CTTGGTCATC GTATCATGGA TTGCCCACTT
    TCTATTGAAG ATAGACAACA GATGGCTGAC AACACGTTTA TTCTGGCTAT TGATGGAGAT
    TCTAAGTTCG AACCTGACGC ACTTCTTCGT CTTCTCCACT TGATGAATGC GAAAAGTGAT
    ATTGGATGTG CTTGTGGAAG AATTCACCCA ATTGGAAATG GTATAATGGT TTGGTATCAG
    AAGTTTGAGT ACGCTATTGC TCATTGGTTT CAAAAGGCTG CTGAACATGT TTTCGGTTGT
    GTCCTTTGTG CGCCTGGATG TTTCTCATTG TTCCGTGCAT CTGCATTGAT GGATGACAAT
    ATCATGCACA AGTATACTAA GACCGCTTCC GAACCACGTC ACTACGTCCA ATATGATCAA
    GGAGAAGACA GATGGTTAAG TACATTGCTT TTGAAACAAG GATATCGTAT CGAATACGCC
    GCTGCGTCTG ATGCTGAAAC ATACGCTCCG GAAGGATTCG AAGAATTTTT CAATCAACGT
    AGACGCTGGA CTCCTTCGTC GATTGCTAAT ACAGTAGATC TTCTGATGGA TTACAAGAGA
    GCTTCAGAAA ATAATGACGC CATTTCATAT GCCTACATCG CCTATCAATT CCTTGTGATC
    TTCTTCTCAA TGCTCGGACC TGCCATTATA TTCACTATGC TCGTATTCGC CCAAGTTGCT
    GCTTTTGAAC TTCGAGGATC TGATGTGATG CTCTATAATG GAATTCCGAT AGGATTTTTC
    ATTGTTCTTT GTTTCACAAC AGAGTCGAAT ATTCAACTTA TCTATGCCAA GTACATGTCG
    ATTGCGTACG CGTTCGTAAT GTTGGCTGTG TTAGTCGCTA CCAGCAGTCA AATTGTTCTC
    GAGACTGTTC TCGCTCCTAC ATCTCTCTTC ATTGTCACAA TGGTCGGAAT CTTCTTCTTT
    GCTGCATGTC TTCATCCAAA AGAATTCACG AATATTATCC ATGGTGTCGT ATTCTTCCTC
    ATGATTCCAT CTACATATGT GTTCCTCACT TTATATTCGC TCATCAATCT CAACGTTATC
    ACGTGGGGAA CTCGTGAAGC TGTCGCTAAG GCAACGGGAC AAAAGACGAA AAAAGCGCCT
    ATGGAACAAT TTATAGACAG AGTGATTGAT ATTGTGAAAA AGGGATTCAG ATTAATCAGT
    TGTCGGGAGA AGAAGGAACA TGAAGAGAGA CGAGAGAAAA TGGAAAAGAA AATGCAGAGA
    ATGGAGCTAG CCTTGAGAAG TATTGAGAGT GGTGCCGACG TGAAGAAAAT TCTCGATGCA
    ACAGAGGAGA AGGAGAAACG TGAAGAAGAA ACTCAAACTG CAGATTTTCC GATTGAAGAG
    AACGTAGAGA AGACTCAAAA AGAGATTCAG AAGGCAAACC GTTATGTGTG GATGACAAGT
    CATAGCTTGA AAGTTTGTGA ACGAGGAAAA CTGAAAAGTG CGGAAAAGGT TTTCTGGAAC
    GAGCTCATCA ATGCATATCT GAAACCGATC AAGACGACGC CAGCTGAAAT GAAAGCCGTC
    GCCGAAGGAT TGGCTTCTCT ACGAAATCAG ATTGCTTTCA CTATTCTTCT CGTTAATTCT
    CTTCTTGCTC TTGCCATCTT TTTGATTCAG AAACACAAAA ATGTGCTCAG CATCAAGTTC
    TCGCCAATCA AAAACTTCCG ATGGACGAAA ATGAATGAGA TGACTGGACA ATACGAGGAA
    ACCGATGAAC CATTAAAAAT AGATCCACTT GGAATGGGAA TTGTTGTTTT CCTTCTAATT
    ATTCTTTTTG TTCAAACTCT CGGAATGCTT CTCCATCGAC TTAACACTAT GATAGGAGCT
    TTCCAAGAAG TGAAAAATTT GTACGAATAC GGAGTTTCAC CAGTGATAAA TACAAAGAAT
    GATGATGAGA GGATTATGAA TAATGCTCGT CTAATGATTA ATTCTCTCGG TGTCAGTACT
    GGACACGCTG CCGACGGCTA TACAAGGCAT CGCGGAGAGG AATCCGATAC CGGCAATGTT
    CTCTACAAAC TTCAAAAGGC TCGTCTCGCA AAGCGAATGC AAAGAAGTGC ACTTTCCACA
    ACTGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.