bub-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	bub-1
Entrez Gene ID	172468
Full Name	Mitotic checkpoint serine/threonine-protein kinase bub-1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_059650.5	NP_492051.1	Mitotic checkpoint serine/threonine-protein kinase bub-1
NM_059650.4	NP_492051.1	Mitotic checkpoint serine/threonine-protein kinase bub-1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The BUB-1/BUB-3 complex promotes timely anaphase onset in Caenorhabditis elegans embryos.
Title: Kinetochore-localized BUB-1/BUB-3 complex promotes anaphase onset in C. elegans.

An interaction between BUB-1 and MAD-1 is essential for spindle checkpoint signaling.
Title: A Bub1-Mad1 interaction targets the Mad1-Mad2 complex to unattached kinetochores to initiate the spindle checkpoint.

C. elegans bub-1 is required both maternally and zygotically
Title: The function of a spindle checkpoint gene bub-1 in C. elegans development.

The following bub-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bub-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa337359	NM_059650.5 Latest version!	Caenorhabditis elegans Mitotic checkpoint serine/threonine-protein kinase bub-1 (bub-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCa337359	NM_059650.4	Caenorhabditis elegans Mitotic checkpoint serine/threonine-protein kinase bub-1 (bub-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bub-1 ( NM_059650.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492051.1 Caenorhabditis elegans Mitotic checkpoint serine/threonine-protein kinase bub-1 (bub-1), mRNA.

Price & Availability↑

CloneID	OCa337359
Clone ID Related Accession (Same CDS sequence)	NM_059650.5 , NM_059650.4
Accession Version	NM_059650.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Mitotic checkpoint serine/threonine-protein kinase bub-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059650.4.

ATGTCCCACA TTAGAGTGGC ATTCGCTCCA CTCGACACCA ATCCGTCGAC ATGTGGTCTT 
GAGACGTTCG CAACGCAAAT CGAAACGTTG TCTCAAATGA TGACCGCGGA GGAAATGAAG 
GCGGAATTAT ACGACTGCGT TAGGAGAATT TTGCGGGAGC AAAAGTTTGA TCAAATTCGT 
GAAAACGAAG CGATGCTCCG TCTCTACAAA GTTATGGGTC GATCTAGTAC AAACCTGAAG 
GGAAGAGGCA TTTATGAACA ACTTTTCAAA AAGGATCATT TCACTGGTTC GCTGAAGTTC 
TATCTGCAAT GGGCAGAAGA ATGTGGAAAA GATCAAATGC TGGAGGAATT CAAAGATGTC 
CTGAAGTTGG CACGAGATCG TCTTTCCGAA AGAATAGAAA TGACAGCAAT TGAATCGGGA 
TTTCGCGATT TGGTCGATGA ATACTTCAAT GGAGAATCTG GAGATATGTT CACACGACCG 
GATGAGACTA TGGATCTTTT CAGATTCAAT GCGGGTTCTA AGAAAAAGCG TCGCTCTTCG 
GTTTGCTTTT TGCAACACAA TGTGCCTATA AATAACTCGG GAAAAGCTGC ATTTGGACCA 
AAAACCAAAA CGGATCTTCG CCAGGCGTGT ATCGACAGGC CAAACTATCA TGGAATATCG 
ATTGAGGAGT TTCGTTTTGC CAAATGGAAG GACACTTTCG GAGAAGATGT TGATGACGAT 
TACCGAAAAC GAAAAGACTC TGGTGTCGTT TTCGTGAAAC ATCAAGTCAT TGATACCGAC 
AGACAAGCCC GCGAAGAAGT TGAAAACCGA TTCAACGCCA ATCTAAATCC TAGAAGACGT 
CATCTTTCAC CAGTCAGTGA GAAAACGGTT GATGATGAGG AGGAAAAGCG AAGCCGGATT 
TATTCGCCGC TGGTTGCAAC GAAGGATGCT CACAGACCTG CACTTCGGAG CAAAATTGAG 
AATCCTCCAG CGACAGTGAC ACTTTCGTCG GATACAAAGT CTGCTTCGGA GAAAGATGTT 
AGTGATTCCG ATGATGCAGA TGATGATGAA AGACTCAAGA TTATGACTGC CGGCAGAAAA 
GATGGTAACC CTCCAGACCG TTCCACAAGC ATATCTTCCA ACTATTCAAC TGCTTCTGCA 
AGAACATCAA AGAGTGGAGC TGGATTGGAT TTGATGGCGG AAAATAAGTG TTTGGAGGCA 
CATGCTATGT TTTCCGACAC TGTACATCTT GCTAGCGAAA AGACAATGGT CCTTGGCGAT 
GATTCTGTCT TCGTTCCAGA AAGATCTTTA GCTACTACGC AGATAGTTAC TGACTTTTCC 
GTGCTCTGTG ATCCTGATCC GACAATGACC ATTACACAGG AGCGTCCGAA AAAAGTGTCG 
AATGGGTTGA ATGTTGTTTA CGATGAGGCA GCCGAACCGG AAGAATCTCA GAAAGTTGAG 
GAATCTGAAG TACAACCCGA AATTGTCCTA GTTTCTCCAG TGACGCAAAC CTCACCAGCT 
ACAATGTTTA ATGATATTTA TGACGATGAA ATCGAGTTTG GCTTTTTCAA ACCGTCTCGT 
GGTAATTTCG TCACATCGAC CCCCGCACAA GGAGTTCATT TGGTCAACAT TGATGAATAT 
TTCGGAAATA AAGAGGAGGA AAGCACTCAC GAACAGGAAG CTCCAGTATT TGTTGCTCCA 
ACCAGCAGTA CTTTCAGTAA ATTAACACGT CGAAAGTCAC TAGCAGCAAA TCAAGCCGTT 
CAGCCCTCAG TCACAGAGTC ATCAAAGCCT GAACGATCAG ATCCTAAAGA TTCATCTATC 
GATTGTTTGA CAGCTAATCT AGGAAGACGT CTTTCAATTG GTGCTGATGA AATTCCAAAT 
CTCACTGAAA ACAACGAATC TGAAATCACT GGTTGCAAGA TTCGTCGGCG CAGTGAAATT 
ATCAAGCAAG GAGACATCAA TCCATGGGAC GAAACTCTTC GAAAAAAATT GATGTGTCTT 
GTGCGTCCTC CCCAGAATAT GCACGAGTTC CAAGAACGAG CACCGAAGAT TCAAGCTCTG 
AGAGACTGCG AGGTTAGCGG AGAAAAGCTC CACATTCAAA CTCTTATTGG TCAAGGTGGA 
TACGCTAAAG TATACCGGGC TGTAACCGAT GATCAAAGGA CAGTAGCTGT GAAGTACGAG 
GTGCCATCAT GTTCGTGGGA AGTGTACATT TGCGACCAAA TGCGGAATCG CCTGAAAGAT 
CGAGGTTTGG AGCTGATGGC CAAATGTTGC ATTATGGAAG TGATGGATGC TTATGTTTAT 
TCAACTGCTT CGCTTCTTGT TAATCAGTAC CACGAATATG GAACGCTGCT TGAATATGCG 
AATAACATGA AGGATCCGAA TTGGCACATA ACCTGCTTCT TGATTACCCA AATGGCCCGA 
GTTGTGAAGG AAGTCCATGC CTCTAAAATT ATTCATGGAG ATATCAAACC GGATAATTTT 
ATGATCACCA GAAAGATCGA TGATAAATGG GGCAAAGATG CTCTGATGAG TAACGACAGC 
TTTGTCATCA AGATTATCGA CTGGGGACGT GCCATTGACA TGATGCCACT GAAGAACCAG 
CGTTTCAAGG GAAGAGCAGG AACAGAAGCG TTTGATTGCC CGGAAATGGT TGACGGTCGT 
TCTTGGACTT ACCAAGCCGA TTACTTCGGA TTCGCCGCAA CAATGGCTGT CGTTGTTGCA 
GGAAAGTATG CACAGTTGAC TGGAGCTAGC GTTGGAGACT ATTCGTTAAA TGTCGACATC 
AAGAGGCGCA ACATTCTTCG CGATGCTTGC TACGATGTCA TTAACAGATT CCTCAACATC 
CCTTCGTGTG ATTCACTTCC GGACTGGAAT ATCCTGATCA AGTCTTTCTC GGAAATCTGG 
AATGAAAAGT TTGAAGCATC GGGATGGCGA CAGGCGGTTT CCAAGTTCAA TGAGGCCTGT 
GATCTTGCCG CCAACCAAAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492051.1
CDS	7..2970
Translation	MSHIRVAFAPLDTNPSTCGLETFATQIETLSQMMTAEEMKAELYDCVRRILREQKFDQIRENEAMLRLYKVMGRSSTNLKGRGIYEQLFKKDHFTGSLKFYLQWAEECGKDQMLEEFKDVLKLARDRLSERIEMTAIESGFRDLVDEYFNGESGDMFTRPDETMDLFRFNAGSKKKRRSSVCFLQHNVPINNSGKAAFGPKTKTDLRQACIDRPNYHGISIEEFRFAKWKDTFGEDVDDDYRKRKDSGVVFVKHQVIDTDRQAREEVENRFNANLNPRRRHLSPVSEKTVDDEEEKRSRIYSPLVATKDAHRPALRSKIENPPATVTLSSDTKSASEKDVSDSDDADDDERLKIMTAGRKDGNPPDRSTSISSNYSTASARTSKSGAGLDLMAENKCLEAHAMFSDTVHLASEKTMVLGDDSVFVPERSLATTQIVTDFSVLCDPDPTMTITQERPKKVSNGLNVVYDEAAEPEESQKVEESEVQPEIVLVSPVTQTSPATMFNDIYDDEIEFGFFKPSRGNFVTSTPAQGVHLVNIDEYFGNKEEESTHEQEAPVFVAPTSSTFSKLTRRKSLAANQAVQPSVTESSKPERSDPKDSSIDCLTANLGRRLSIGADEIPNLTENNESEITGCKIRRRSEIIKQGDINPWDETLRKKLMCLVRPPQNMHEFQERAPKIQALRDCEVSGEKLHIQTLIGQGGYAKVYRAVTDDQRTVAVKYEVPSCSWEVYICDQMRNRLKDRGLELMAKCCIMEVMDAYVYSTASLLVNQYHEYGTLLEYANNMKDPNWHITCFLITQMARVVKEVHASKIIHGDIKPDNFMITRKIDDKWGKDALMSNDSFVIKIIDWGRAIDMMPLKNQRFKGRAGTEAFDCPEMVDGRSWTYQADYFGFAATMAVVVAGKYAQLTGASVGDYSLNVDIKRRNILRDACYDVINRFLNIPSCDSLPDWNILIKSFSEIWNEKFEASGWRQAVSKFNEACDLAANQK

Target ORF information:

RefSeq Version	NM_059650.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Mitotic checkpoint serine/threonine-protein kinase bub-1 (bub-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059650.5

ORF Insert Sequence:

ATGTCCCACA TTAGAGTGGC ATTCGCTCCA CTCGACACCA ATCCGTCGAC ATGTGGTCTT 
GAGACGTTCG CAACGCAAAT CGAAACGTTG TCTCAAATGA TGACCGCGGA GGAAATGAAG 
GCGGAATTAT ACGACTGCGT TAGGAGAATT TTGCGGGAGC AAAAGTTTGA TCAAATTCGT 
GAAAACGAAG CGATGCTCCG TCTCTACAAA GTTATGGGTC GATCTAGTAC AAACCTGAAG 
GGAAGAGGCA TTTATGAACA ACTTTTCAAA AAGGATCATT TCACTGGTTC GCTGAAGTTC 
TATCTGCAAT GGGCAGAAGA ATGTGGAAAA GATCAAATGC TGGAGGAATT CAAAGATGTC 
CTGAAGTTGG CACGAGATCG TCTTTCCGAA AGAATAGAAA TGACAGCAAT TGAATCGGGA 
TTTCGCGATT TGGTCGATGA ATACTTCAAT GGAGAATCTG GAGATATGTT CACACGACCG 
GATGAGACTA TGGATCTTTT CAGATTCAAT GCGGGTTCTA AGAAAAAGCG TCGCTCTTCG 
GTTTGCTTTT TGCAACACAA TGTGCCTATA AATAACTCGG GAAAAGCTGC ATTTGGACCA 
AAAACCAAAA CGGATCTTCG CCAGGCGTGT ATCGACAGGC CAAACTATCA TGGAATATCG 
ATTGAGGAGT TTCGTTTTGC CAAATGGAAG GACACTTTCG GAGAAGATGT TGATGACGAT 
TACCGAAAAC GAAAAGACTC TGGTGTCGTT TTCGTGAAAC ATCAAGTCAT TGATACCGAC 
AGACAAGCCC GCGAAGAAGT TGAAAACCGA TTCAACGCCA ATCTAAATCC TAGAAGACGT 
CATCTTTCAC CAGTCAGTGA GAAAACGGTT GATGATGAGG AGGAAAAGCG AAGCCGGATT 
TATTCGCCGC TGGTTGCAAC GAAGGATGCT CACAGACCTG CACTTCGGAG CAAAATTGAG 
AATCCTCCAG CGACAGTGAC ACTTTCGTCG GATACAAAGT CTGCTTCGGA GAAAGATGTT 
AGTGATTCCG ATGATGCAGA TGATGATGAA AGACTCAAGA TTATGACTGC CGGCAGAAAA 
GATGGTAACC CTCCAGACCG TTCCACAAGC ATATCTTCCA ACTATTCAAC TGCTTCTGCA 
AGAACATCAA AGAGTGGAGC TGGATTGGAT TTGATGGCGG AAAATAAGTG TTTGGAGGCA 
CATGCTATGT TTTCCGACAC TGTACATCTT GCTAGCGAAA AGACAATGGT CCTTGGCGAT 
GATTCTGTCT TCGTTCCAGA AAGATCTTTA GCTACTACGC AGATAGTTAC TGACTTTTCC 
GTGCTCTGTG ATCCTGATCC GACAATGACC ATTACACAGG AGCGTCCGAA AAAAGTGTCG 
AATGGGTTGA ATGTTGTTTA CGATGAGGCA GCCGAACCGG AAGAATCTCA GAAAGTTGAG 
GAATCTGAAG TACAACCCGA AATTGTCCTA GTTTCTCCAG TGACGCAAAC CTCACCAGCT 
ACAATGTTTA ATGATATTTA TGACGATGAA ATCGAGTTTG GCTTTTTCAA ACCGTCTCGT 
GGTAATTTCG TCACATCGAC CCCCGCACAA GGAGTTCATT TGGTCAACAT TGATGAATAT 
TTCGGAAATA AAGAGGAGGA AAGCACTCAC GAACAGGAAG CTCCAGTATT TGTTGCTCCA 
ACCAGCAGTA CTTTCAGTAA ATTAACACGT CGAAAGTCAC TAGCAGCAAA TCAAGCCGTT 
CAGCCCTCAG TCACAGAGTC ATCAAAGCCT GAACGATCAG ATCCTAAAGA TTCATCTATC 
GATTGTTTGA CAGCTAATCT AGGAAGACGT CTTTCAATTG GTGCTGATGA AATTCCAAAT 
CTCACTGAAA ACAACGAATC TGAAATCACT GGTTGCAAGA TTCGTCGGCG CAGTGAAATT 
ATCAAGCAAG GAGACATCAA TCCATGGGAC GAAACTCTTC GAAAAAAATT GATGTGTCTT 
GTGCGTCCTC CCCAGAATAT GCACGAGTTC CAAGAACGAG CACCGAAGAT TCAAGCTCTG 
AGAGACTGCG AGGTTAGCGG AGAAAAGCTC CACATTCAAA CTCTTATTGG TCAAGGTGGA 
TACGCTAAAG TATACCGGGC TGTAACCGAT GATCAAAGGA CAGTAGCTGT GAAGTACGAG 
GTGCCATCAT GTTCGTGGGA AGTGTACATT TGCGACCAAA TGCGGAATCG CCTGAAAGAT 
CGAGGTTTGG AGCTGATGGC CAAATGTTGC ATTATGGAAG TGATGGATGC TTATGTTTAT 
TCAACTGCTT CGCTTCTTGT TAATCAGTAC CACGAATATG GAACGCTGCT TGAATATGCG 
AATAACATGA AGGATCCGAA TTGGCACATA ACCTGCTTCT TGATTACCCA AATGGCCCGA 
GTTGTGAAGG AAGTCCATGC CTCTAAAATT ATTCATGGAG ATATCAAACC GGATAATTTT 
ATGATCACCA GAAAGATCGA TGATAAATGG GGCAAAGATG CTCTGATGAG TAACGACAGC 
TTTGTCATCA AGATTATCGA CTGGGGACGT GCCATTGACA TGATGCCACT GAAGAACCAG 
CGTTTCAAGG GAAGAGCAGG AACAGAAGCG TTTGATTGCC CGGAAATGGT TGACGGTCGT 
TCTTGGACTT ACCAAGCCGA TTACTTCGGA TTCGCCGCAA CAATGGCTGT CGTTGTTGCA 
GGAAAGTATG CACAGTTGAC TGGAGCTAGC GTTGGAGACT ATTCGTTAAA TGTCGACATC 
AAGAGGCGCA ACATTCTTCG CGATGCTTGC TACGATGTCA TTAACAGATT CCTCAACATC 
CCTTCGTGTG ATTCACTTCC GGACTGGAAT ATCCTGATCA AGTCTTTCTC GGAAATCTGG 
AATGAAAAGT TTGAAGCATC GGGATGGCGA CAGGCGGTTT CCAAGTTCAA TGAGGCCTGT 
GATCTTGCCG CCAACCAAAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub-1 ( NM_059650.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492051.1 Caenorhabditis elegans Mitotic checkpoint serine/threonine-protein kinase bub-1 (bub-1), partial mRNA.

Price & Availability↑

CloneID	OCa337359
Clone ID Related Accession (Same CDS sequence)	NM_059650.5 , NM_059650.4
Accession Version	NM_059650.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Mitotic checkpoint serine/threonine-protein kinase bub-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_059650.3. COMPLETENESS: incomplete on both ends.

ATGTCCCACA TTAGAGTGGC ATTCGCTCCA CTCGACACCA ATCCGTCGAC ATGTGGTCTT 
GAGACGTTCG CAACGCAAAT CGAAACGTTG TCTCAAATGA TGACCGCGGA GGAAATGAAG 
GCGGAATTAT ACGACTGCGT TAGGAGAATT TTGCGGGAGC AAAAGTTTGA TCAAATTCGT 
GAAAACGAAG CGATGCTCCG TCTCTACAAA GTTATGGGTC GATCTAGTAC AAACCTGAAG 
GGAAGAGGCA TTTATGAACA ACTTTTCAAA AAGGATCATT TCACTGGTTC GCTGAAGTTC 
TATCTGCAAT GGGCAGAAGA ATGTGGAAAA GATCAAATGC TGGAGGAATT CAAAGATGTC 
CTGAAGTTGG CACGAGATCG TCTTTCCGAA AGAATAGAAA TGACAGCAAT TGAATCGGGA 
TTTCGCGATT TGGTCGATGA ATACTTCAAT GGAGAATCTG GAGATATGTT CACACGACCG 
GATGAGACTA TGGATCTTTT CAGATTCAAT GCGGGTTCTA AGAAAAAGCG TCGCTCTTCG 
GTTTGCTTTT TGCAACACAA TGTGCCTATA AATAACTCGG GAAAAGCTGC ATTTGGACCA 
AAAACCAAAA CGGATCTTCG CCAGGCGTGT ATCGACAGGC CAAACTATCA TGGAATATCG 
ATTGAGGAGT TTCGTTTTGC CAAATGGAAG GACACTTTCG GAGAAGATGT TGATGACGAT 
TACCGAAAAC GAAAAGACTC TGGTGTCGTT TTCGTGAAAC ATCAAGTCAT TGATACCGAC 
AGACAAGCCC GCGAAGAAGT TGAAAACCGA TTCAACGCCA ATCTAAATCC TAGAAGACGT 
CATCTTTCAC CAGTCAGTGA GAAAACGGTT GATGATGAGG AGGAAAAGCG AAGCCGGATT 
TATTCGCCGC TGGTTGCAAC GAAGGATGCT CACAGACCTG CACTTCGGAG CAAAATTGAG 
AATCCTCCAG CGACAGTGAC ACTTTCGTCG GATACAAAGT CTGCTTCGGA GAAAGATGTT 
AGTGATTCCG ATGATGCAGA TGATGATGAA AGACTCAAGA TTATGACTGC CGGCAGAAAA 
GATGGTAACC CTCCAGACCG TTCCACAAGC ATATCTTCCA ACTATTCAAC TGCTTCTGCA 
AGAACATCAA AGAGTGGAGC TGGATTGGAT TTGATGGCGG AAAATAAGTG TTTGGAGGCA 
CATGCTATGT TTTCCGACAC TGTACATCTT GCTAGCGAAA AGACAATGGT CCTTGGCGAT 
GATTCTGTCT TCGTTCCAGA AAGATCTTTA GCTACTACGC AGATAGTTAC TGACTTTTCC 
GTGCTCTGTG ATCCTGATCC GACAATGACC ATTACACAGG AGCGTCCGAA AAAAGTGTCG 
AATGGGTTGA ATGTTGTTTA CGATGAGGCA GCCGAACCGG AAGAATCTCA GAAAGTTGAG 
GAATCTGAAG TACAACCCGA AATTGTCCTA GTTTCTCCAG TGACGCAAAC CTCACCAGCT 
ACAATGTTTA ATGATATTTA TGACGATGAA ATCGAGTTTG GCTTTTTCAA ACCGTCTCGT 
GGTAATTTCG TCACATCGAC CCCCGCACAA GGAGTTCATT TGGTCAACAT TGATGAATAT 
TTCGGAAATA AAGAGGAGGA AAGCACTCAC GAACAGGAAG CTCCAGTATT TGTTGCTCCA 
ACCAGCAGTA CTTTCAGTAA ATTAACACGT CGAAAGTCAC TAGCAGCAAA TCAAGCCGTT 
CAGCCCTCAG TCACAGAGTC ATCAAAGCCT GAACGATCAG ATCCTAAAGA TTCATCTATC 
GATTGTTTGA CAGCTAATCT AGGAAGACGT CTTTCAATTG GTGCTGATGA AATTCCAAAT 
CTCACTGAAA ACAACGAATC TGAAATCACT GGTTGCAAGA TTCGTCGGCG CAGTGAAATT 
ATCAAGCAAG GAGACATCAA TCCATGGGAC GAAACTCTTC GAAAAAAATT GATGTGTCTT 
GTGCGTCCTC CCCAGAATAT GCACGAGTTC CAAGAACGAG CACCGAAGAT TCAAGCTCTG 
AGAGACTGCG AGGTTAGCGG AGAAAAGCTC CACATTCAAA CTCTTATTGG TCAAGGTGGA 
TACGCTAAAG TATACCGGGC TGTAACCGAT GATCAAAGGA CAGTAGCTGT GAAGTACGAG 
GTGCCATCAT GTTCGTGGGA AGTGTACATT TGCGACCAAA TGCGGAATCG CCTGAAAGAT 
CGAGGTTTGG AGCTGATGGC CAAATGTTGC ATTATGGAAG TGATGGATGC TTATGTTTAT 
TCAACTGCTT CGCTTCTTGT TAATCAGTAC CACGAATATG GAACGCTGCT TGAATATGCG 
AATAACATGA AGGATCCGAA TTGGCACATA ACCTGCTTCT TGATTACCCA AATGGCCCGA 
GTTGTGAAGG AAGTCCATGC CTCTAAAATT ATTCATGGAG ATATCAAACC GGATAATTTT 
ATGATCACCA GAAAGATCGA TGATAAATGG GGCAAAGATG CTCTGATGAG TAACGACAGC 
TTTGTCATCA AGATTATCGA CTGGGGACGT GCCATTGACA TGATGCCACT GAAGAACCAG 
CGTTTCAAGG GAAGAGCAGG AACAGAAGCG TTTGATTGCC CGGAAATGGT TGACGGTCGT 
TCTTGGACTT ACCAAGCCGA TTACTTCGGA TTCGCCGCAA CAATGGCTGT CGTTGTTGCA 
GGAAAGTATG CACAGTTGAC TGGAGCTAGC GTTGGAGACT ATTCGTTAAA TGTCGACATC 
AAGAGGCGCA ACATTCTTCG CGATGCTTGC TACGATGTCA TTAACAGATT CCTCAACATC 
CCTTCGTGTG ATTCACTTCC GGACTGGAAT ATCCTGATCA AGTCTTTCTC GGAAATCTGG 
AATGAAAAGT TTGAAGCATC GGGATGGCGA CAGGCGGTTT CCAAGTTCAA TGAGGCCTGT 
GATCTTGCCG CCAACCAAAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492051.1
CDS	1..2964
Translation	MSHIRVAFAPLDTNPSTCGLETFATQIETLSQMMTAEEMKAELYDCVRRILREQKFDQIRENEAMLRLYKVMGRSSTNLKGRGIYEQLFKKDHFTGSLKFYLQWAEECGKDQMLEEFKDVLKLARDRLSERIEMTAIESGFRDLVDEYFNGESGDMFTRPDETMDLFRFNAGSKKKRRSSVCFLQHNVPINNSGKAAFGPKTKTDLRQACIDRPNYHGISIEEFRFAKWKDTFGEDVDDDYRKRKDSGVVFVKHQVIDTDRQAREEVENRFNANLNPRRRHLSPVSEKTVDDEEEKRSRIYSPLVATKDAHRPALRSKIENPPATVTLSSDTKSASEKDVSDSDDADDDERLKIMTAGRKDGNPPDRSTSISSNYSTASARTSKSGAGLDLMAENKCLEAHAMFSDTVHLASEKTMVLGDDSVFVPERSLATTQIVTDFSVLCDPDPTMTITQERPKKVSNGLNVVYDEAAEPEESQKVEESEVQPEIVLVSPVTQTSPATMFNDIYDDEIEFGFFKPSRGNFVTSTPAQGVHLVNIDEYFGNKEEESTHEQEAPVFVAPTSSTFSKLTRRKSLAANQAVQPSVTESSKPERSDPKDSSIDCLTANLGRRLSIGADEIPNLTENNESEITGCKIRRRSEIIKQGDINPWDETLRKKLMCLVRPPQNMHEFQERAPKIQALRDCEVSGEKLHIQTLIGQGGYAKVYRAVTDDQRTVAVKYEVPSCSWEVYICDQMRNRLKDRGLELMAKCCIMEVMDAYVYSTASLLVNQYHEYGTLLEYANNMKDPNWHITCFLITQMARVVKEVHASKIIHGDIKPDNFMITRKIDDKWGKDALMSNDSFVIKIIDWGRAIDMMPLKNQRFKGRAGTEAFDCPEMVDGRSWTYQADYFGFAATMAVVVAGKYAQLTGASVGDYSLNVDIKRRNILRDACYDVINRFLNIPSCDSLPDWNILIKSFSEIWNEKFEASGWRQAVSKFNEACDLAANQK

Target ORF information:

RefSeq Version	NM_059650.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Mitotic checkpoint serine/threonine-protein kinase bub-1 (bub-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059650.4

ORF Insert Sequence:

ATGTCCCACA TTAGAGTGGC ATTCGCTCCA CTCGACACCA ATCCGTCGAC ATGTGGTCTT 
GAGACGTTCG CAACGCAAAT CGAAACGTTG TCTCAAATGA TGACCGCGGA GGAAATGAAG 
GCGGAATTAT ACGACTGCGT TAGGAGAATT TTGCGGGAGC AAAAGTTTGA TCAAATTCGT 
GAAAACGAAG CGATGCTCCG TCTCTACAAA GTTATGGGTC GATCTAGTAC AAACCTGAAG 
GGAAGAGGCA TTTATGAACA ACTTTTCAAA AAGGATCATT TCACTGGTTC GCTGAAGTTC 
TATCTGCAAT GGGCAGAAGA ATGTGGAAAA GATCAAATGC TGGAGGAATT CAAAGATGTC 
CTGAAGTTGG CACGAGATCG TCTTTCCGAA AGAATAGAAA TGACAGCAAT TGAATCGGGA 
TTTCGCGATT TGGTCGATGA ATACTTCAAT GGAGAATCTG GAGATATGTT CACACGACCG 
GATGAGACTA TGGATCTTTT CAGATTCAAT GCGGGTTCTA AGAAAAAGCG TCGCTCTTCG 
GTTTGCTTTT TGCAACACAA TGTGCCTATA AATAACTCGG GAAAAGCTGC ATTTGGACCA 
AAAACCAAAA CGGATCTTCG CCAGGCGTGT ATCGACAGGC CAAACTATCA TGGAATATCG 
ATTGAGGAGT TTCGTTTTGC CAAATGGAAG GACACTTTCG GAGAAGATGT TGATGACGAT 
TACCGAAAAC GAAAAGACTC TGGTGTCGTT TTCGTGAAAC ATCAAGTCAT TGATACCGAC 
AGACAAGCCC GCGAAGAAGT TGAAAACCGA TTCAACGCCA ATCTAAATCC TAGAAGACGT 
CATCTTTCAC CAGTCAGTGA GAAAACGGTT GATGATGAGG AGGAAAAGCG AAGCCGGATT 
TATTCGCCGC TGGTTGCAAC GAAGGATGCT CACAGACCTG CACTTCGGAG CAAAATTGAG 
AATCCTCCAG CGACAGTGAC ACTTTCGTCG GATACAAAGT CTGCTTCGGA GAAAGATGTT 
AGTGATTCCG ATGATGCAGA TGATGATGAA AGACTCAAGA TTATGACTGC CGGCAGAAAA 
GATGGTAACC CTCCAGACCG TTCCACAAGC ATATCTTCCA ACTATTCAAC TGCTTCTGCA 
AGAACATCAA AGAGTGGAGC TGGATTGGAT TTGATGGCGG AAAATAAGTG TTTGGAGGCA 
CATGCTATGT TTTCCGACAC TGTACATCTT GCTAGCGAAA AGACAATGGT CCTTGGCGAT 
GATTCTGTCT TCGTTCCAGA AAGATCTTTA GCTACTACGC AGATAGTTAC TGACTTTTCC 
GTGCTCTGTG ATCCTGATCC GACAATGACC ATTACACAGG AGCGTCCGAA AAAAGTGTCG 
AATGGGTTGA ATGTTGTTTA CGATGAGGCA GCCGAACCGG AAGAATCTCA GAAAGTTGAG 
GAATCTGAAG TACAACCCGA AATTGTCCTA GTTTCTCCAG TGACGCAAAC CTCACCAGCT 
ACAATGTTTA ATGATATTTA TGACGATGAA ATCGAGTTTG GCTTTTTCAA ACCGTCTCGT 
GGTAATTTCG TCACATCGAC CCCCGCACAA GGAGTTCATT TGGTCAACAT TGATGAATAT 
TTCGGAAATA AAGAGGAGGA AAGCACTCAC GAACAGGAAG CTCCAGTATT TGTTGCTCCA 
ACCAGCAGTA CTTTCAGTAA ATTAACACGT CGAAAGTCAC TAGCAGCAAA TCAAGCCGTT 
CAGCCCTCAG TCACAGAGTC ATCAAAGCCT GAACGATCAG ATCCTAAAGA TTCATCTATC 
GATTGTTTGA CAGCTAATCT AGGAAGACGT CTTTCAATTG GTGCTGATGA AATTCCAAAT 
CTCACTGAAA ACAACGAATC TGAAATCACT GGTTGCAAGA TTCGTCGGCG CAGTGAAATT 
ATCAAGCAAG GAGACATCAA TCCATGGGAC GAAACTCTTC GAAAAAAATT GATGTGTCTT 
GTGCGTCCTC CCCAGAATAT GCACGAGTTC CAAGAACGAG CACCGAAGAT TCAAGCTCTG 
AGAGACTGCG AGGTTAGCGG AGAAAAGCTC CACATTCAAA CTCTTATTGG TCAAGGTGGA 
TACGCTAAAG TATACCGGGC TGTAACCGAT GATCAAAGGA CAGTAGCTGT GAAGTACGAG 
GTGCCATCAT GTTCGTGGGA AGTGTACATT TGCGACCAAA TGCGGAATCG CCTGAAAGAT 
CGAGGTTTGG AGCTGATGGC CAAATGTTGC ATTATGGAAG TGATGGATGC TTATGTTTAT 
TCAACTGCTT CGCTTCTTGT TAATCAGTAC CACGAATATG GAACGCTGCT TGAATATGCG 
AATAACATGA AGGATCCGAA TTGGCACATA ACCTGCTTCT TGATTACCCA AATGGCCCGA 
GTTGTGAAGG AAGTCCATGC CTCTAAAATT ATTCATGGAG ATATCAAACC GGATAATTTT 
ATGATCACCA GAAAGATCGA TGATAAATGG GGCAAAGATG CTCTGATGAG TAACGACAGC 
TTTGTCATCA AGATTATCGA CTGGGGACGT GCCATTGACA TGATGCCACT GAAGAACCAG 
CGTTTCAAGG GAAGAGCAGG AACAGAAGCG TTTGATTGCC CGGAAATGGT TGACGGTCGT 
TCTTGGACTT ACCAAGCCGA TTACTTCGGA TTCGCCGCAA CAATGGCTGT CGTTGTTGCA 
GGAAAGTATG CACAGTTGAC TGGAGCTAGC GTTGGAGACT ATTCGTTAAA TGTCGACATC 
AAGAGGCGCA ACATTCTTCG CGATGCTTGC TACGATGTCA TTAACAGATT CCTCAACATC 
CCTTCGTGTG ATTCACTTCC GGACTGGAAT ATCCTGATCA AGTCTTTCTC GGAAATCTGG 
AATGAAAAGT TTGAAGCATC GGGATGGCGA CAGGCGGTTT CCAAGTTCAA TGAGGCCTGT 
GATCTTGCCG CCAACCAAAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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bub-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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