smg-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	smg-1
Entrez Gene ID	172418
Full Name	Serine/threonine-protein kinase smg-1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001025882.2	NP_001021053.1	Serine/threonine-protein kinase smg-1
NM_001374908.1	NP_001361976.1	Serine/threonine-protein kinase smg-1
NM_001025883.3	NP_001021054.1	Serine/threonine-protein kinase smg-1
NM_001025882.3	NP_001021053.1	Serine/threonine-protein kinase smg-1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel role is reported for smg-1 in lifespan and oxidative stress resistance in Caenorhabditis elegans.
Title: A novel role for the SMG-1 kinase in lifespan and oxidative stress resistance in Caenorhabditis elegans.

smg-1 encodes a protein kinase of the phosphatidylinositol kinase superfamily of protein kinases; SMG-1 kinase activity is required in vivo for nonsense-mediated mRNA decay and in vitro for SMG-2 phosphorylation
Title: SMG-1 is a phosphatidylinositol kinase-related protein kinase required for nonsense-mediated mRNA Decay in Caenorhabditis elegans.

The following smg-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smg-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa336706	NM_001025882.3 Latest version!	Caenorhabditis elegans Serine/threonine-protein kinase smg-1 (smg-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa336707	NM_001025883.3 Latest version!	Caenorhabditis elegans Serine/threonine-protein kinase smg-1 (smg-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa850853	NM_001374908.1 Latest version!	Caenorhabditis elegans Serine/threonine-protein kinase smg-1 (smg-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCa336706	NM_001025882.2	Caenorhabditis elegans Serine/threonine-protein kinase smg-1 (smg-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smg-1 ( NM_001025882.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021053.1 Caenorhabditis elegans Serine/threonine-protein kinase smg-1 (smg-1), partial mRNA.

Price & Availability↑

CloneID	OCa336706
Clone ID Related Accession (Same CDS sequence)	NM_001025882.2 , NM_001025882.3
Accession Version	NM_001025882.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase smg-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_001025882.1. COMPLETENESS: incomplete on both ends.

ATGATAACAT CGAGAAACAA TGATATTGGG AACCTGATAG AACAGTTTCG TCAACGTGAC 
ACGCCACAGA AGGAACGGAA AGCGATTCTC GCTAGAATCG AGGAGATTCT TCAAACTACG 
AAAAATGTAG AGAGTCTTTG TGTAAAATGG ACTTATCTTT TGGATAACTT GTGTTGGCCT 
AGTCTCACAA AACACGATCG GAATGACATG AAAACGCTGG CTGGAAAAGT GATACGACTT 
GTTGGAGTTT TACTCTTTGA TACTGAATCC TATCCAGAGT TTCTGATTTA TCTTGGAACA 
TTATATCAAA GTGTTACGAA GAAAAGTGAA GAAACACGTG CGGATATCGT GTTTTCAGTG 
TATTTCATCG TTGGAGTCAT CAGTCAAAAA ACGGAGAATC GTCTAATTGC TACCGACACT 
GAAAATGTCG AAAAATCATT AGACTGGATA ATCAAAGTGC TTCCGAATTC ATCAATATCA 
GTTTATAACC ACTGTTTGAA AGGATTTGTA CTTGTTGCAA ACACATTTCC AAATGTTTAT 
GCTGCAATGT TTGAGTCAAC CCTCCGAGCA ATTCTGACCA ACCTTCCCGA TTTCAACAGT 
CATGAAAAGA ATTTTGAGCT TCTCATTGAT ACAGTGATGC GATTTTCTGA TCAACTTAAT 
GAGAAGCCTC ATTTGGCAGA AGAAATGGTT CGAATTATTC GACCGGATAT CAAAAAGAAT 
GGATTGGGAA ATATGAGAGA ATTGAAGAAA CGAATGAAAC TGACAATGGC ACTCGTGAAA 
ATGGCGAAAA GTCAAAAAAT GCTTGAGGAG ACTAATCAGA TGATTTCTGA AATGTCTATC 
GAACTCGAGG AAAACGGAGG AAAATGGTCA TCAGCTTCAC TTATAACTAT CGTTTGTGAT 
GTTTTCAATG AGCTCTTGAT ATTGGGAAAA GATGATGTAA AATTGCAAAA AGGAGTTGAA 
GAATCATTGT GCAATGTTCT GAAAGACTTG AATCTTTCTA ATCAGAGTAC TATGGAGAAA 
CAAGCATTTT TCAATTCTCT TGCAAAGATT GTCAAACAAC TTCCAGCAGA GTCACAAGTC 
AAAACTAGAG TTCATCAGAT TGTTTTCAAC ACGGAAACTG GACTTTTCAC ACCCAAAAAC 
CGCGATAATC GAATGTTTGG ACATAATATG ATATACAAGG ATCTTATCAA TCTTGTCTCT 
GTGCTTCTTA CGCCTACATC ACTCAATCAT CTACAAGCGA CATATACGGA TTTGAGGAAA 
ATAATGATAG ATAGCATGTC ACGATTGAAG CAAAGTGAAG ATACTCCGTA TAGTGATAAT 
ATTCGATGGA ATGAATCAAT CCTTTTGCTT TTCTTTTCGT CACTTCAAAG TATATCATGT 
GCCAAGAGTT CTCTTATTGT GATGATGGGA ATCCGTCCGT CAATTTTTGA ATTCTTCTCA 
TCGGAACTTC CACTCACAGA ATATTGGCTT GCTTCAAATC ATCCAGAAGT CTATCACCTA 
TTTATCACAA TATTTGTTGG ACATTTGAAA GCTCACGACT TCTACATTGT TCAATCTGAT 
TATATTGTTC GTGGTGATAG CATTGGACAA AGTATAGGAC AAACGAAACG AGATTATGCA 
CGGAAACAAG TTGTTGCTCT ACAGAAAATT ATCAACAATT TTGGAGACAA ATTGTGGAAG 
AAGACTAGAC TGATGATATC TTCATGGCTT CACTCTCTCA TTGCAACTGC TTGTGAACAT 
CAAATCGGAA GTGATAGTTT CAGTCAACGA GAATGGGTTC GATTAAGGAA TACTGTCATT 
CATCAGTCGG TGCTCACATG GAATAATGAA TGTGTAAATC AAGCATTGAC CATCCTCTCC 
ACTGCTACAA AATGGTCAGA ACTGACGTCA GACATCCATC GAGATATTGC GGATAAAACG 
AAAAAAGCAA AGTGGAAGGA GGCAACGACA ATTTGGGAAT CTGGAGATTG TAATACTTAT 
ATCAGGCAAT CCATGTCTAC AGTCTATCAA ATGTCACAGG AACGACAACA GAAGACAATT 
ACATCGACAA GTTTTGGAGC CGAAGAGTTT ATAATAATTA CGAATTTCTT GTTGAAACAG 
GCAACACCAA CTACTTTCAA AAAAGGTCAA AACTCATGGA TGGATGAAGT ACTGGAAACA 
TTCACACAAG GATGTCGTAC GCTTGAAAAA CCCGATTCAT ACGTTCCAGA GACATTTATC 
GAAAAATGGG ATTGGATTAT TAATCAGACT GCAAACTTTT GCATTGTGAA TAAAATGAAG 
ACACCATTAG GAAAACCAAT GCAGACTTTT GCAGCTTTTG AGAATGAAAT TAAACGACTT 
GCGAAAGAAG TGATTGTTCG AAAGAATAGT GATAAGAAGT TGAATAAATC ATCTACAGAA 
GATCCAAATC AATCTCCACC ACTCAAATAT TCCGTTCAAT GGCTTCGTGT TCATTTACTT 
CTAAAATTGA TTGTAGTTTT GGAAAAACTT ATGAACTCAG CCATTCATGG TGGTTCTTCC 
GTTTTCAATT TGACTGAAAT TCCAGTGTCC TCTCGCCAAT TTTTCACAGT GAACGCAGCA 
AGTTGTGAGG TTTGGCTGAA TCGAGTATAC TATCCTGCTC TTCTTGTCGC ATATTTCAAT 
GGATATTATG GACTTGTTAT TCGATTTGGT TCAAATGCTC TTAGCCATTT CGCGAGACAA 
AAAGACGGAG ATAATGATAA AAAGATCGTG AATGGCGTTT GTACAGCAAG TTTAATGTCA 
CTTTCAATGG CAGTTCTCGG TGAACCAATG GAAATTGTTG GATTACGGAG AAAAGTTCGA 
GAAGAATTCG GAACTGACAT GGGACAATCA TTGATGGAGG CACTCGGAGA AATGGCTAAT 
GCTAGATATG AGACAGCTCT GGTTGCCTTG GAAGCAGTTC TTGTGACAGA TGCAGCAACA 
AATGAAACAT TGAAAATGAT CATTCAACTA GCAATGGTTG ACATTTTGAA CCGCATACGC 
CTCCCACAGG CTACCGATTA TTATAAGGTT GTCTTATTTG GTGAAGAATC AAATGATTCC 
ACAATTACTG AAGACTTTCG ATCAGTTGAG TTACTCACGA AATTCGAGAA GCTCAACAAT 
ACAGTGAACG AAAAGCGTCA AGTTGTTGAT TGGAGTGCTC GAGAACGTTT CCAATTTGTT 
GAATCCGCAT TTTCACAGAC TATGAGAAGA ACCGAACTTC TCGACATTCA AAAAGATTTT 
TCCGCGATGG GAGCACTTGC ATTGAGTGCT GATTCATCGT GTAAATTGTA TTCGGACATT 
TCATCAACAT CTCTCATAAT TGCAAATCTT GTCAATAAAA TGACTGGTGT CTCACAATGG 
AAGAATAAAC TCACTGATAC GGAAATATTC GATAGAAATG AAGAAGGAAA TGATGGTGAT 
AAGTTGGCAA TATGTCGAAA GTTAATGCAT TGGGGACGAC ACACAAAGAG TAATCGCGGA 
CAGTCATGCG CTGCACACAG TGAAATAATT CGTTTATCCC GTAAAACTTC AAATTGTGAA 
CTCGCGTTCT TTCACATAAA TTCAGCTATT CGAGGTGAAA AACTAGCTGC TTGGCAGAGA 
TTAGAAGTGG AAAGACAGCG ACTGAAACTA GTGAAAACTC AAAATTTGGA TGTGCGAATT 
CGTGAAATGA ATGAAGTGTT CGGATCCTTG GCTGAAGTTT TTACGACAAG TTGTCAACTG 
AAATCAGACT TTCAAATGGT TGATGGAATG ATTAAAGAAA AAATGATCTC CGAAGGATAT 
AATGAAGATA TTGCAAAGCG TGAGGAGCAC ATGAGTCGAG CCAGTATTCA ACTTGCAGAC 
TTTTTCCAAT CACTTCCAGA ACTTGAAAAT GTACTTGCGC CCAACCTGTT CCCGACAATT 
ATTTGGAGTG AACTACAACG TCGATCAGAT AGTTTATCTG CTGGATATCA TGGAATAGTT 
GGATCTCTCT TTCATTTGGC ATCTGAAATG TGTCCATCTC TCGCTAAAGC TCATCTCAAA 
ATGGCCAGAT GGGCATATGA AATCGCTAAA ATCAAGAATT TCCCTGCTGA AAATTTAAGT 
TTCTACAAAT TTGGAAAAGA CGAAACTGAA AACGAGGAGC TATTGAAGAG CCTGGAAGCG 
ACGAGTCTTG TTAATTTGGA AAAGCTTGTT CGAGCTGCAA TTTCTGATGA TTTGAGAGCA 
AACAACATTC TGGCACCCAA TAGTCACTTT ATGCACATTT GGAAAATGGT TCGGGATCAT 
CGCACGAAAT TCTTGTCAAT TGCTGTGACT TCATATTTCC AATTTATTCA GAATATGTCC 
GGTGATTGTG ATAATCTACC GTACTCGAAA AAAGAGGAGA CCACTTTGGC GACATTGAGA 
ATTCTAGAAC TTCTTGTCAA ACATGGCGAT GTTCTCATTG ATGTAATAAA TGATGGACTA 
AATAAGACTA ATGTACATAT TTGGAAAGAA ATTCTTCCTC AATTGTTTGC TCGCTTGTCC 
CATCCATCTG AACATATTCG AAAAACTCTT GTTGATCTAA TTTCAAAAAT CTGTACAGCT 
GCACCACATG CAGTCGTTTT TCAAGTTGTT TCTGGTGCTG CATCATCTTC TACAGACGGT 
GAAGAACTTG AAGAGCAGCA GAATGATGAT CGTAACCGAG TACGTGCATG CTGTGAGAAA 
TTGGAGACAA ATATGTCACA ATCATATCCG AATCTTGTGA AAGATGTACG GCAGTTTGTA 
GCAGAACTTG AACGAATTAA TCTTTTGAAT GAAGAGAAAT GGTCTGTTGT CATGGGAACA 
ATGGAACATG AAATGGAAAA ACGATTATCA TTGATTAGAA CTGAAAATGC GAAAACTGAA 
TCGGCTCTTC ATTTAACAGC TTCAGTCAAA AATGATATTA TTGTGAAAAG AACCCAATTG 
TTGACTAGAC AGATATTTGA TGTACTCGAT GAGCTCTACC AACAAACAGT CATTGAGCCT 
CCAAAGAGCA AGAATGAAGA AGAGTTTGTA ACAGCTTTTG CCGAAGTACT CACAAACGCT 
TTTCAAGAGT CCAGAATATC ACGTACCACT TCTCCGGAAA AGTCATGGAT TCCATTTAAA 
AATCTCATTG CCAATTTTGT GCATCGAAAC AGCAAAAAAG GAATGCAAAC ATTTGAAACG 
GAAGACATTT CTCCGTACCT CGCATCTCTT TCCAATTCGT GTGTTCCAAT GCCAGGTCAA 
GAATCTGTCG AGTTTGATAG AGTTGTTTCG ATATCTCGTG TTGCTCGACA AGTCACAATT 
CTGCCCACGA AAACTCGCCC GAAAAAGCTG GGATTTGTCG GATCTGATGG AAAACAAGTT 
GCATTTTTAT TCAAAGGACG CGAAGATCTT CATCTTGATG AACGAGTCAT GCAATTCCTC 
CGATTATGTA ATGTGATGCT TCAACCAGGA AAAGGGAAAC ATCGACAATC GGTTGCCGCG 
TATCAGGCAC ATCATTATGC TGTTATTCCA CTTGGACCAC GTTCTGGACT TATCAAATGG 
GTTGAAGGTG CTACACCAAT GTTCCACATA TATCGTAAAT GGCAGATGAA AGAGAAAGCA 
CTCAAACAAG CGACCAAAAA GAACGGAGAA ACAGTTCCTG AAATCGAAAG ACCCAGCAAC 
ATGTATCACA ATATGATTCG TCTAGCATTC GCAGATCATA AAATCGATTC CTCCATCACC 
TCGGATCGTT CTAAATGGCC AGCAGAAATT TTGGAAGAAG TATTTGAAAG TCTCACAGCA 
AAAACACCAA CAGATTTAAT CAGTCGTGAG CTTTGGATGC GTGCGAATGA TGCAACAACT 
TGGTGGTCGG TTACAAAACG ATATTCAAGA TCACTGGCAG TCATGAGTAT GGTTGGATCA 
GTTTTAGGAC TCGGTGATCG ACATTTGGAC AATTTATTGG TTGATTTGAA GTGGGGTCAT 
GTAGTACATA TTGACTACAA CATTTGCTTT GACAAGGGAA AGAATCTGCG TATCCCCGAA 
ACAGTCCCAT TCCGTTTGAC ACGTAACATG CGTCACGCTC TCGGACCATC TGAAATGTAT 
GGTACCTTCC GTGAATCTTG TGTTCACGTG CTCTCAACTC TTCGTTCTGG TCATCAAGTT 
CTTACAATGC TTCTCGATGC ATTTGTATTC GATCCGCTTG TCGATTGGAC ATCACATGAA 
CATACAGCAA CTTCTGGTGT CTCATTGGCA CTACAATTGG CTGTTTATGG AAGTAATTGG 
AAGACAAAAG CTAAAGAAAG ATTGACCGAT GCGATGGAGT TACTCAATTT GAGAATGTCT 
GAGGTTCAAA CCCTTTGGCT TGCAAATCGT GATGACCTTC TCCACTGGAT GAAACAAGTG 
ACCGAATGTC TTTTGATTGA AAATTCGATG CTTGGAGCAA ATGCCATATA TGCTCAGCAA 
CGTGTGAAAG CTGGAACGGA ACTTCGTGAG GCTGTGACAC GTCATCATGC ACTTGCCAAA 
GAGCTTCGTC CATTGATAAG AGTAATTGGA AAGGAACGAG AAGAGTTTGC AGATTATTTG 
AAGTTTTACA AGCAGGCATT ATTTGATCCA CTTTTGAAAG GACACTCGGC TCTCCGTAAC 
GAACTCGATA TTGATACATG TGTGTACAAT TTCAACATTG TCATGCAAAA TATAGATAAT 
GTGTTTGGAG CACTCGTCAA TCTGTCATTC ACCCCGATTG AGACTATTAC ATCCCGTACT 
TCACAGCAAG AATTCAAACC ACCTCCCGGA CTTGAAAATG TATGGGTTGT GAAACAAGAT 
CAACAGGAGA ATTCTCAAGC GCGTGAAGTT GTCCGACGTG TTGAACGGCG TCTCAACGGA 
TGGCTCGATG GATCGGCAGG ACCAGATAGG AAATTGTCTC CTCGCGAAGA GGCCGACATC 
CTAATCGCTG AAGCTACATC CACACCAAAT TTATCTCAAA TGTACGAAGG ATGGACTGCT 
TGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021053.1
CDS	1..6969
Translation	MITSRNNDIGNLIEQFRQRDTPQKERKAILARIEEILQTTKNVESLCVKWTYLLDNLCWPSLTKHDRNDMKTLAGKVIRLVGVLLFDTESYPEFLIYLGTLYQSVTKKSEETRADIVFSVYFIVGVISQKTENRLIATDTENVEKSLDWIIKVLPNSSISVYNHCLKGFVLVANTFPNVYAAMFESTLRAILTNLPDFNSHEKNFELLIDTVMRFSDQLNEKPHLAEEMVRIIRPDIKKNGLGNMRELKKRMKLTMALVKMAKSQKMLEETNQMISEMSIELEENGGKWSSASLITIVCDVFNELLILGKDDVKLQKGVEESLCNVLKDLNLSNQSTMEKQAFFNSLAKIVKQLPAESQVKTRVHQIVFNTETGLFTPKNRDNRMFGHNMIYKDLINLVSVLLTPTSLNHLQATYTDLRKIMIDSMSRLKQSEDTPYSDNIRWNESILLLFFSSLQSISCAKSSLIVMMGIRPSIFEFFSSELPLTEYWLASNHPEVYHLFITIFVGHLKAHDFYIVQSDYIVRGDSIGQSIGQTKRDYARKQVVALQKIINNFGDKLWKKTRLMISSWLHSLIATACEHQIGSDSFSQREWVRLRNTVIHQSVLTWNNECVNQALTILSTATKWSELTSDIHRDIADKTKKAKWKEATTIWESGDCNTYIRQSMSTVYQMSQERQQKTITSTSFGAEEFIIITNFLLKQATPTTFKKGQNSWMDEVLETFTQGCRTLEKPDSYVPETFIEKWDWIINQTANFCIVNKMKTPLGKPMQTFAAFENEIKRLAKEVIVRKNSDKKLNKSSTEDPNQSPPLKYSVQWLRVHLLLKLIVVLEKLMNSAIHGGSSVFNLTEIPVSSRQFFTVNAASCEVWLNRVYYPALLVAYFNGYYGLVIRFGSNALSHFARQKDGDNDKKIVNGVCTASLMSLSMAVLGEPMEIVGLRRKVREEFGTDMGQSLMEALGEMANARYETALVALEAVLVTDAATNETLKMIIQLAMVDILNRIRLPQATDYYKVVLFGEESNDSTITEDFRSVELLTKFEKLNNTVNEKRQVVDWSARERFQFVESAFSQTMRRTELLDIQKDFSAMGALALSADSSCKLYSDISSTSLIIANLVNKMTGVSQWKNKLTDTEIFDRNEEGNDGDKLAICRKLMHWGRHTKSNRGQSCAAHSEIIRLSRKTSNCELAFFHINSAIRGEKLAAWQRLEVERQRLKLVKTQNLDVRIREMNEVFGSLAEVFTTSCQLKSDFQMVDGMIKEKMISEGYNEDIAKREEHMSRASIQLADFFQSLPELENVLAPNLFPTIIWSELQRRSDSLSAGYHGIVGSLFHLASEMCPSLAKAHLKMARWAYEIAKIKNFPAENLSFYKFGKDETENEELLKSLEATSLVNLEKLVRAAISDDLRANNILAPNSHFMHIWKMVRDHRTKFLSIAVTSYFQFIQNMSGDCDNLPYSKKEETTLATLRILELLVKHGDVLIDVINDGLNKTNVHIWKEILPQLFARLSHPSEHIRKTLVDLISKICTAAPHAVVFQVVSGAASSSTDGEELEEQQNDDRNRVRACCEKLETNMSQSYPNLVKDVRQFVAELERINLLNEEKWSVVMGTMEHEMEKRLSLIRTENAKTESALHLTASVKNDIIVKRTQLLTRQIFDVLDELYQQTVIEPPKSKNEEEFVTAFAEVLTNAFQESRISRTTSPEKSWIPFKNLIANFVHRNSKKGMQTFETEDISPYLASLSNSCVPMPGQESVEFDRVVSISRVARQVTILPTKTRPKKLGFVGSDGKQVAFLFKGREDLHLDERVMQFLRLCNVMLQPGKGKHRQSVAAYQAHHYAVIPLGPRSGLIKWVEGATPMFHIYRKWQMKEKALKQATKKNGETVPEIERPSNMYHNMIRLAFADHKIDSSITSDRSKWPAEILEEVFESLTAKTPTDLISRELWMRANDATTWWSVTKRYSRSLAVMSMVGSVLGLGDRHLDNLLVDLKWGHVVHIDYNICFDKGKNLRIPETVPFRLTRNMRHALGPSEMYGTFRESCVHVLSTLRSGHQVLTMLLDAFVFDPLVDWTSHEHTATSGVSLALQLAVYGSNWKTKAKERLTDAMELLNLRMSEVQTLWLANRDDLLHWMKQVTECLLIENSMLGANAIYAQQRVKAGTELREAVTRHHALAKELRPLIRVIGKEREEFADYLKFYKQALFDPLLKGHSALRNELDIDTCVYNFNIVMQNIDNVFGALVNLSFTPIETITSRTSQQEFKPPPGLENVWVVKQDQQENSQAREVVRRVERRLNGWLDGSAGPDRKLSPREEADILIAEATSTPNLSQMYEGWTAWV

Target ORF information:

RefSeq Version	NM_001025882.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase smg-1 (smg-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025882.2

ORF Insert Sequence:

ATGATAACAT CGAGAAACAA TGATATTGGG AACCTGATAG AACAGTTTCG TCAACGTGAC 
ACGCCACAGA AGGAACGGAA AGCGATTCTC GCTAGAATCG AGGAGATTCT TCAAACTACG 
AAAAATGTAG AGAGTCTTTG TGTAAAATGG ACTTATCTTT TGGATAACTT GTGTTGGCCT 
AGTCTCACAA AACACGATCG GAATGACATG AAAACGCTGG CTGGAAAAGT GATACGACTT 
GTTGGAGTTT TACTCTTTGA TACTGAATCC TATCCAGAGT TTCTGATTTA TCTTGGAACA 
TTATATCAAA GTGTTACGAA GAAAAGTGAA GAAACACGTG CGGATATCGT GTTTTCAGTG 
TATTTCATCG TTGGAGTCAT CAGTCAAAAA ACGGAGAATC GTCTAATTGC TACCGACACT 
GAAAATGTCG AAAAATCATT AGACTGGATA ATCAAAGTGC TTCCGAATTC ATCAATATCA 
GTTTATAACC ACTGTTTGAA AGGATTTGTA CTTGTTGCAA ACACATTTCC AAATGTTTAT 
GCTGCAATGT TTGAGTCAAC CCTCCGAGCA ATTCTGACCA ACCTTCCCGA TTTCAACAGT 
CATGAAAAGA ATTTTGAGCT TCTCATTGAT ACAGTGATGC GATTTTCTGA TCAACTTAAT 
GAGAAGCCTC ATTTGGCAGA AGAAATGGTT CGAATTATTC GACCGGATAT CAAAAAGAAT 
GGATTGGGAA ATATGAGAGA ATTGAAGAAA CGAATGAAAC TGACAATGGC ACTCGTGAAA 
ATGGCGAAAA GTCAAAAAAT GCTTGAGGAG ACTAATCAGA TGATTTCTGA AATGTCTATC 
GAACTCGAGG AAAACGGAGG AAAATGGTCA TCAGCTTCAC TTATAACTAT CGTTTGTGAT 
GTTTTCAATG AGCTCTTGAT ATTGGGAAAA GATGATGTAA AATTGCAAAA AGGAGTTGAA 
GAATCATTGT GCAATGTTCT GAAAGACTTG AATCTTTCTA ATCAGAGTAC TATGGAGAAA 
CAAGCATTTT TCAATTCTCT TGCAAAGATT GTCAAACAAC TTCCAGCAGA GTCACAAGTC 
AAAACTAGAG TTCATCAGAT TGTTTTCAAC ACGGAAACTG GACTTTTCAC ACCCAAAAAC 
CGCGATAATC GAATGTTTGG ACATAATATG ATATACAAGG ATCTTATCAA TCTTGTCTCT 
GTGCTTCTTA CGCCTACATC ACTCAATCAT CTACAAGCGA CATATACGGA TTTGAGGAAA 
ATAATGATAG ATAGCATGTC ACGATTGAAG CAAAGTGAAG ATACTCCGTA TAGTGATAAT 
ATTCGATGGA ATGAATCAAT CCTTTTGCTT TTCTTTTCGT CACTTCAAAG TATATCATGT 
GCCAAGAGTT CTCTTATTGT GATGATGGGA ATCCGTCCGT CAATTTTTGA ATTCTTCTCA 
TCGGAACTTC CACTCACAGA ATATTGGCTT GCTTCAAATC ATCCAGAAGT CTATCACCTA 
TTTATCACAA TATTTGTTGG ACATTTGAAA GCTCACGACT TCTACATTGT TCAATCTGAT 
TATATTGTTC GTGGTGATAG CATTGGACAA AGTATAGGAC AAACGAAACG AGATTATGCA 
CGGAAACAAG TTGTTGCTCT ACAGAAAATT ATCAACAATT TTGGAGACAA ATTGTGGAAG 
AAGACTAGAC TGATGATATC TTCATGGCTT CACTCTCTCA TTGCAACTGC TTGTGAACAT 
CAAATCGGAA GTGATAGTTT CAGTCAACGA GAATGGGTTC GATTAAGGAA TACTGTCATT 
CATCAGTCGG TGCTCACATG GAATAATGAA TGTGTAAATC AAGCATTGAC CATCCTCTCC 
ACTGCTACAA AATGGTCAGA ACTGACGTCA GACATCCATC GAGATATTGC GGATAAAACG 
AAAAAAGCAA AGTGGAAGGA GGCAACGACA ATTTGGGAAT CTGGAGATTG TAATACTTAT 
ATCAGGCAAT CCATGTCTAC AGTCTATCAA ATGTCACAGG AACGACAACA GAAGACAATT 
ACATCGACAA GTTTTGGAGC CGAAGAGTTT ATAATAATTA CGAATTTCTT GTTGAAACAG 
GCAACACCAA CTACTTTCAA AAAAGGTCAA AACTCATGGA TGGATGAAGT ACTGGAAACA 
TTCACACAAG GATGTCGTAC GCTTGAAAAA CCCGATTCAT ACGTTCCAGA GACATTTATC 
GAAAAATGGG ATTGGATTAT TAATCAGACT GCAAACTTTT GCATTGTGAA TAAAATGAAG 
ACACCATTAG GAAAACCAAT GCAGACTTTT GCAGCTTTTG AGAATGAAAT TAAACGACTT 
GCGAAAGAAG TGATTGTTCG AAAGAATAGT GATAAGAAGT TGAATAAATC ATCTACAGAA 
GATCCAAATC AATCTCCACC ACTCAAATAT TCCGTTCAAT GGCTTCGTGT TCATTTACTT 
CTAAAATTGA TTGTAGTTTT GGAAAAACTT ATGAACTCAG CCATTCATGG TGGTTCTTCC 
GTTTTCAATT TGACTGAAAT TCCAGTGTCC TCTCGCCAAT TTTTCACAGT GAACGCAGCA 
AGTTGTGAGG TTTGGCTGAA TCGAGTATAC TATCCTGCTC TTCTTGTCGC ATATTTCAAT 
GGATATTATG GACTTGTTAT TCGATTTGGT TCAAATGCTC TTAGCCATTT CGCGAGACAA 
AAAGACGGAG ATAATGATAA AAAGATCGTG AATGGCGTTT GTACAGCAAG TTTAATGTCA 
CTTTCAATGG CAGTTCTCGG TGAACCAATG GAAATTGTTG GATTACGGAG AAAAGTTCGA 
GAAGAATTCG GAACTGACAT GGGACAATCA TTGATGGAGG CACTCGGAGA AATGGCTAAT 
GCTAGATATG AGACAGCTCT GGTTGCCTTG GAAGCAGTTC TTGTGACAGA TGCAGCAACA 
AATGAAACAT TGAAAATGAT CATTCAACTA GCAATGGTTG ACATTTTGAA CCGCATACGC 
CTCCCACAGG CTACCGATTA TTATAAGGTT GTCTTATTTG GTGAAGAATC AAATGATTCC 
ACAATTACTG AAGACTTTCG ATCAGTTGAG TTACTCACGA AATTCGAGAA GCTCAACAAT 
ACAGTGAACG AAAAGCGTCA AGTTGTTGAT TGGAGTGCTC GAGAACGTTT CCAATTTGTT 
GAATCCGCAT TTTCACAGAC TATGAGAAGA ACCGAACTTC TCGACATTCA AAAAGATTTT 
TCCGCGATGG GAGCACTTGC ATTGAGTGCT GATTCATCGT GTAAATTGTA TTCGGACATT 
TCATCAACAT CTCTCATAAT TGCAAATCTT GTCAATAAAA TGACTGGTGT CTCACAATGG 
AAGAATAAAC TCACTGATAC GGAAATATTC GATAGAAATG AAGAAGGAAA TGATGGTGAT 
AAGTTGGCAA TATGTCGAAA GTTAATGCAT TGGGGACGAC ACACAAAGAG TAATCGCGGA 
CAGTCATGCG CTGCACACAG TGAAATAATT CGTTTATCCC GTAAAACTTC AAATTGTGAA 
CTCGCGTTCT TTCACATAAA TTCAGCTATT CGAGGTGAAA AACTAGCTGC TTGGCAGAGA 
TTAGAAGTGG AAAGACAGCG ACTGAAACTA GTGAAAACTC AAAATTTGGA TGTGCGAATT 
CGTGAAATGA ATGAAGTGTT CGGATCCTTG GCTGAAGTTT TTACGACAAG TTGTCAACTG 
AAATCAGACT TTCAAATGGT TGATGGAATG ATTAAAGAAA AAATGATCTC CGAAGGATAT 
AATGAAGATA TTGCAAAGCG TGAGGAGCAC ATGAGTCGAG CCAGTATTCA ACTTGCAGAC 
TTTTTCCAAT CACTTCCAGA ACTTGAAAAT GTACTTGCGC CCAACCTGTT CCCGACAATT 
ATTTGGAGTG AACTACAACG TCGATCAGAT AGTTTATCTG CTGGATATCA TGGAATAGTT 
GGATCTCTCT TTCATTTGGC ATCTGAAATG TGTCCATCTC TCGCTAAAGC TCATCTCAAA 
ATGGCCAGAT GGGCATATGA AATCGCTAAA ATCAAGAATT TCCCTGCTGA AAATTTAAGT 
TTCTACAAAT TTGGAAAAGA CGAAACTGAA AACGAGGAGC TATTGAAGAG CCTGGAAGCG 
ACGAGTCTTG TTAATTTGGA AAAGCTTGTT CGAGCTGCAA TTTCTGATGA TTTGAGAGCA 
AACAACATTC TGGCACCCAA TAGTCACTTT ATGCACATTT GGAAAATGGT TCGGGATCAT 
CGCACGAAAT TCTTGTCAAT TGCTGTGACT TCATATTTCC AATTTATTCA GAATATGTCC 
GGTGATTGTG ATAATCTACC GTACTCGAAA AAAGAGGAGA CCACTTTGGC GACATTGAGA 
ATTCTAGAAC TTCTTGTCAA ACATGGCGAT GTTCTCATTG ATGTAATAAA TGATGGACTA 
AATAAGACTA ATGTACATAT TTGGAAAGAA ATTCTTCCTC AATTGTTTGC TCGCTTGTCC 
CATCCATCTG AACATATTCG AAAAACTCTT GTTGATCTAA TTTCAAAAAT CTGTACAGCT 
GCACCACATG CAGTCGTTTT TCAAGTTGTT TCTGGTGCTG CATCATCTTC TACAGACGGT 
GAAGAACTTG AAGAGCAGCA GAATGATGAT CGTAACCGAG TACGTGCATG CTGTGAGAAA 
TTGGAGACAA ATATGTCACA ATCATATCCG AATCTTGTGA AAGATGTACG GCAGTTTGTA 
GCAGAACTTG AACGAATTAA TCTTTTGAAT GAAGAGAAAT GGTCTGTTGT CATGGGAACA 
ATGGAACATG AAATGGAAAA ACGATTATCA TTGATTAGAA CTGAAAATGC GAAAACTGAA 
TCGGCTCTTC ATTTAACAGC TTCAGTCAAA AATGATATTA TTGTGAAAAG AACCCAATTG 
TTGACTAGAC AGATATTTGA TGTACTCGAT GAGCTCTACC AACAAACAGT CATTGAGCCT 
CCAAAGAGCA AGAATGAAGA AGAGTTTGTA ACAGCTTTTG CCGAAGTACT CACAAACGCT 
TTTCAAGAGT CCAGAATATC ACGTACCACT TCTCCGGAAA AGTCATGGAT TCCATTTAAA 
AATCTCATTG CCAATTTTGT GCATCGAAAC AGCAAAAAAG GAATGCAAAC ATTTGAAACG 
GAAGACATTT CTCCGTACCT CGCATCTCTT TCCAATTCGT GTGTTCCAAT GCCAGGTCAA 
GAATCTGTCG AGTTTGATAG AGTTGTTTCG ATATCTCGTG TTGCTCGACA AGTCACAATT 
CTGCCCACGA AAACTCGCCC GAAAAAGCTG GGATTTGTCG GATCTGATGG AAAACAAGTT 
GCATTTTTAT TCAAAGGACG CGAAGATCTT CATCTTGATG AACGAGTCAT GCAATTCCTC 
CGATTATGTA ATGTGATGCT TCAACCAGGA AAAGGGAAAC ATCGACAATC GGTTGCCGCG 
TATCAGGCAC ATCATTATGC TGTTATTCCA CTTGGACCAC GTTCTGGACT TATCAAATGG 
GTTGAAGGTG CTACACCAAT GTTCCACATA TATCGTAAAT GGCAGATGAA AGAGAAAGCA 
CTCAAACAAG CGACCAAAAA GAACGGAGAA ACAGTTCCTG AAATCGAAAG ACCCAGCAAC 
ATGTATCACA ATATGATTCG TCTAGCATTC GCAGATCATA AAATCGATTC CTCCATCACC 
TCGGATCGTT CTAAATGGCC AGCAGAAATT TTGGAAGAAG TATTTGAAAG TCTCACAGCA 
AAAACACCAA CAGATTTAAT CAGTCGTGAG CTTTGGATGC GTGCGAATGA TGCAACAACT 
TGGTGGTCGG TTACAAAACG ATATTCAAGA TCACTGGCAG TCATGAGTAT GGTTGGATCA 
GTTTTAGGAC TCGGTGATCG ACATTTGGAC AATTTATTGG TTGATTTGAA GTGGGGTCAT 
GTAGTACATA TTGACTACAA CATTTGCTTT GACAAGGGAA AGAATCTGCG TATCCCCGAA 
ACAGTCCCAT TCCGTTTGAC ACGTAACATG CGTCACGCTC TCGGACCATC TGAAATGTAT 
GGTACCTTCC GTGAATCTTG TGTTCACGTG CTCTCAACTC TTCGTTCTGG TCATCAAGTT 
CTTACAATGC TTCTCGATGC ATTTGTATTC GATCCGCTTG TCGATTGGAC ATCACATGAA 
CATACAGCAA CTTCTGGTGT CTCATTGGCA CTACAATTGG CTGTTTATGG AAGTAATTGG 
AAGACAAAAG CTAAAGAAAG ATTGACCGAT GCGATGGAGT TACTCAATTT GAGAATGTCT 
GAGGTTCAAA CCCTTTGGCT TGCAAATCGT GATGACCTTC TCCACTGGAT GAAACAAGTG 
ACCGAATGTC TTTTGATTGA AAATTCGATG CTTGGAGCAA ATGCCATATA TGCTCAGCAA 
CGTGTGAAAG CTGGAACGGA ACTTCGTGAG GCTGTGACAC GTCATCATGC ACTTGCCAAA 
GAGCTTCGTC CATTGATAAG AGTAATTGGA AAGGAACGAG AAGAGTTTGC AGATTATTTG 
AAGTTTTACA AGCAGGCATT ATTTGATCCA CTTTTGAAAG GACACTCGGC TCTCCGTAAC 
GAACTCGATA TTGATACATG TGTGTACAAT TTCAACATTG TCATGCAAAA TATAGATAAT 
GTGTTTGGAG CACTCGTCAA TCTGTCATTC ACCCCGATTG AGACTATTAC ATCCCGTACT 
TCACAGCAAG AATTCAAACC ACCTCCCGGA CTTGAAAATG TATGGGTTGT GAAACAAGAT 
CAACAGGAGA ATTCTCAAGC GCGTGAAGTT GTCCGACGTG TTGAACGGCG TCTCAACGGA 
TGGCTCGATG GATCGGCAGG ACCAGATAGG AAATTGTCTC CTCGCGAAGA GGCCGACATC 
CTAATCGCTG AAGCTACATC CACACCAAAT TTATCTCAAA TGTACGAAGG ATGGACTGCT 
TGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smg-1 ( NM_001025882.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021053.1 Caenorhabditis elegans Serine/threonine-protein kinase smg-1 (smg-1), mRNA.

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CloneID	OCa336706
Clone ID Related Accession (Same CDS sequence)	NM_001025882.2 , NM_001025882.3
Accession Version	NM_001025882.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase smg-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001025882.2.

ATGATAACAT CGAGAAACAA TGATATTGGG AACCTGATAG AACAGTTTCG TCAACGTGAC 
ACGCCACAGA AGGAACGGAA AGCGATTCTC GCTAGAATCG AGGAGATTCT TCAAACTACG 
AAAAATGTAG AGAGTCTTTG TGTAAAATGG ACTTATCTTT TGGATAACTT GTGTTGGCCT 
AGTCTCACAA AACACGATCG GAATGACATG AAAACGCTGG CTGGAAAAGT GATACGACTT 
GTTGGAGTTT TACTCTTTGA TACTGAATCC TATCCAGAGT TTCTGATTTA TCTTGGAACA 
TTATATCAAA GTGTTACGAA GAAAAGTGAA GAAACACGTG CGGATATCGT GTTTTCAGTG 
TATTTCATCG TTGGAGTCAT CAGTCAAAAA ACGGAGAATC GTCTAATTGC TACCGACACT 
GAAAATGTCG AAAAATCATT AGACTGGATA ATCAAAGTGC TTCCGAATTC ATCAATATCA 
GTTTATAACC ACTGTTTGAA AGGATTTGTA CTTGTTGCAA ACACATTTCC AAATGTTTAT 
GCTGCAATGT TTGAGTCAAC CCTCCGAGCA ATTCTGACCA ACCTTCCCGA TTTCAACAGT 
CATGAAAAGA ATTTTGAGCT TCTCATTGAT ACAGTGATGC GATTTTCTGA TCAACTTAAT 
GAGAAGCCTC ATTTGGCAGA AGAAATGGTT CGAATTATTC GACCGGATAT CAAAAAGAAT 
GGATTGGGAA ATATGAGAGA ATTGAAGAAA CGAATGAAAC TGACAATGGC ACTCGTGAAA 
ATGGCGAAAA GTCAAAAAAT GCTTGAGGAG ACTAATCAGA TGATTTCTGA AATGTCTATC 
GAACTCGAGG AAAACGGAGG AAAATGGTCA TCAGCTTCAC TTATAACTAT CGTTTGTGAT 
GTTTTCAATG AGCTCTTGAT ATTGGGAAAA GATGATGTAA AATTGCAAAA AGGAGTTGAA 
GAATCATTGT GCAATGTTCT GAAAGACTTG AATCTTTCTA ATCAGAGTAC TATGGAGAAA 
CAAGCATTTT TCAATTCTCT TGCAAAGATT GTCAAACAAC TTCCAGCAGA GTCACAAGTC 
AAAACTAGAG TTCATCAGAT TGTTTTCAAC ACGGAAACTG GACTTTTCAC ACCCAAAAAC 
CGCGATAATC GAATGTTTGG ACATAATATG ATATACAAGG ATCTTATCAA TCTTGTCTCT 
GTGCTTCTTA CGCCTACATC ACTCAATCAT CTACAAGCGA CATATACGGA TTTGAGGAAA 
ATAATGATAG ATAGCATGTC ACGATTGAAG CAAAGTGAAG ATACTCCGTA TAGTGATAAT 
ATTCGATGGA ATGAATCAAT CCTTTTGCTT TTCTTTTCGT CACTTCAAAG TATATCATGT 
GCCAAGAGTT CTCTTATTGT GATGATGGGA ATCCGTCCGT CAATTTTTGA ATTCTTCTCA 
TCGGAACTTC CACTCACAGA ATATTGGCTT GCTTCAAATC ATCCAGAAGT CTATCACCTA 
TTTATCACAA TATTTGTTGG ACATTTGAAA GCTCACGACT TCTACATTGT TCAATCTGAT 
TATATTGTTC GTGGTGATAG CATTGGACAA AGTATAGGAC AAACGAAACG AGATTATGCA 
CGGAAACAAG TTGTTGCTCT ACAGAAAATT ATCAACAATT TTGGAGACAA ATTGTGGAAG 
AAGACTAGAC TGATGATATC TTCATGGCTT CACTCTCTCA TTGCAACTGC TTGTGAACAT 
CAAATCGGAA GTGATAGTTT CAGTCAACGA GAATGGGTTC GATTAAGGAA TACTGTCATT 
CATCAGTCGG TGCTCACATG GAATAATGAA TGTGTAAATC AAGCATTGAC CATCCTCTCC 
ACTGCTACAA AATGGTCAGA ACTGACGTCA GACATCCATC GAGATATTGC GGATAAAACG 
AAAAAAGCAA AGTGGAAGGA GGCAACGACA ATTTGGGAAT CTGGAGATTG TAATACTTAT 
ATCAGGCAAT CCATGTCTAC AGTCTATCAA ATGTCACAGG AACGACAACA GAAGACAATT 
ACATCGACAA GTTTTGGAGC CGAAGAGTTT ATAATAATTA CGAATTTCTT GTTGAAACAG 
GCAACACCAA CTACTTTCAA AAAAGGTCAA AACTCATGGA TGGATGAAGT ACTGGAAACA 
TTCACACAAG GATGTCGTAC GCTTGAAAAA CCCGATTCAT ACGTTCCAGA GACATTTATC 
GAAAAATGGG ATTGGATTAT TAATCAGACT GCAAACTTTT GCATTGTGAA TAAAATGAAG 
ACACCATTAG GAAAACCAAT GCAGACTTTT GCAGCTTTTG AGAATGAAAT TAAACGACTT 
GCGAAAGAAG TGATTGTTCG AAAGAATAGT GATAAGAAGT TGAATAAATC ATCTACAGAA 
GATCCAAATC AATCTCCACC ACTCAAATAT TCCGTTCAAT GGCTTCGTGT TCATTTACTT 
CTAAAATTGA TTGTAGTTTT GGAAAAACTT ATGAACTCAG CCATTCATGG TGGTTCTTCC 
GTTTTCAATT TGACTGAAAT TCCAGTGTCC TCTCGCCAAT TTTTCACAGT GAACGCAGCA 
AGTTGTGAGG TTTGGCTGAA TCGAGTATAC TATCCTGCTC TTCTTGTCGC ATATTTCAAT 
GGATATTATG GACTTGTTAT TCGATTTGGT TCAAATGCTC TTAGCCATTT CGCGAGACAA 
AAAGACGGAG ATAATGATAA AAAGATCGTG AATGGCGTTT GTACAGCAAG TTTAATGTCA 
CTTTCAATGG CAGTTCTCGG TGAACCAATG GAAATTGTTG GATTACGGAG AAAAGTTCGA 
GAAGAATTCG GAACTGACAT GGGACAATCA TTGATGGAGG CACTCGGAGA AATGGCTAAT 
GCTAGATATG AGACAGCTCT GGTTGCCTTG GAAGCAGTTC TTGTGACAGA TGCAGCAACA 
AATGAAACAT TGAAAATGAT CATTCAACTA GCAATGGTTG ACATTTTGAA CCGCATACGC 
CTCCCACAGG CTACCGATTA TTATAAGGTT GTCTTATTTG GTGAAGAATC AAATGATTCC 
ACAATTACTG AAGACTTTCG ATCAGTTGAG TTACTCACGA AATTCGAGAA GCTCAACAAT 
ACAGTGAACG AAAAGCGTCA AGTTGTTGAT TGGAGTGCTC GAGAACGTTT CCAATTTGTT 
GAATCCGCAT TTTCACAGAC TATGAGAAGA ACCGAACTTC TCGACATTCA AAAAGATTTT 
TCCGCGATGG GAGCACTTGC ATTGAGTGCT GATTCATCGT GTAAATTGTA TTCGGACATT 
TCATCAACAT CTCTCATAAT TGCAAATCTT GTCAATAAAA TGACTGGTGT CTCACAATGG 
AAGAATAAAC TCACTGATAC GGAAATATTC GATAGAAATG AAGAAGGAAA TGATGGTGAT 
AAGTTGGCAA TATGTCGAAA GTTAATGCAT TGGGGACGAC ACACAAAGAG TAATCGCGGA 
CAGTCATGCG CTGCACACAG TGAAATAATT CGTTTATCCC GTAAAACTTC AAATTGTGAA 
CTCGCGTTCT TTCACATAAA TTCAGCTATT CGAGGTGAAA AACTAGCTGC TTGGCAGAGA 
TTAGAAGTGG AAAGACAGCG ACTGAAACTA GTGAAAACTC AAAATTTGGA TGTGCGAATT 
CGTGAAATGA ATGAAGTGTT CGGATCCTTG GCTGAAGTTT TTACGACAAG TTGTCAACTG 
AAATCAGACT TTCAAATGGT TGATGGAATG ATTAAAGAAA AAATGATCTC CGAAGGATAT 
AATGAAGATA TTGCAAAGCG TGAGGAGCAC ATGAGTCGAG CCAGTATTCA ACTTGCAGAC 
TTTTTCCAAT CACTTCCAGA ACTTGAAAAT GTACTTGCGC CCAACCTGTT CCCGACAATT 
ATTTGGAGTG AACTACAACG TCGATCAGAT AGTTTATCTG CTGGATATCA TGGAATAGTT 
GGATCTCTCT TTCATTTGGC ATCTGAAATG TGTCCATCTC TCGCTAAAGC TCATCTCAAA 
ATGGCCAGAT GGGCATATGA AATCGCTAAA ATCAAGAATT TCCCTGCTGA AAATTTAAGT 
TTCTACAAAT TTGGAAAAGA CGAAACTGAA AACGAGGAGC TATTGAAGAG CCTGGAAGCG 
ACGAGTCTTG TTAATTTGGA AAAGCTTGTT CGAGCTGCAA TTTCTGATGA TTTGAGAGCA 
AACAACATTC TGGCACCCAA TAGTCACTTT ATGCACATTT GGAAAATGGT TCGGGATCAT 
CGCACGAAAT TCTTGTCAAT TGCTGTGACT TCATATTTCC AATTTATTCA GAATATGTCC 
GGTGATTGTG ATAATCTACC GTACTCGAAA AAAGAGGAGA CCACTTTGGC GACATTGAGA 
ATTCTAGAAC TTCTTGTCAA ACATGGCGAT GTTCTCATTG ATGTAATAAA TGATGGACTA 
AATAAGACTA ATGTACATAT TTGGAAAGAA ATTCTTCCTC AATTGTTTGC TCGCTTGTCC 
CATCCATCTG AACATATTCG AAAAACTCTT GTTGATCTAA TTTCAAAAAT CTGTACAGCT 
GCACCACATG CAGTCGTTTT TCAAGTTGTT TCTGGTGCTG CATCATCTTC TACAGACGGT 
GAAGAACTTG AAGAGCAGCA GAATGATGAT CGTAACCGAG TACGTGCATG CTGTGAGAAA 
TTGGAGACAA ATATGTCACA ATCATATCCG AATCTTGTGA AAGATGTACG GCAGTTTGTA 
GCAGAACTTG AACGAATTAA TCTTTTGAAT GAAGAGAAAT GGTCTGTTGT CATGGGAACA 
ATGGAACATG AAATGGAAAA ACGATTATCA TTGATTAGAA CTGAAAATGC GAAAACTGAA 
TCGGCTCTTC ATTTAACAGC TTCAGTCAAA AATGATATTA TTGTGAAAAG AACCCAATTG 
TTGACTAGAC AGATATTTGA TGTACTCGAT GAGCTCTACC AACAAACAGT CATTGAGCCT 
CCAAAGAGCA AGAATGAAGA AGAGTTTGTA ACAGCTTTTG CCGAAGTACT CACAAACGCT 
TTTCAAGAGT CCAGAATATC ACGTACCACT TCTCCGGAAA AGTCATGGAT TCCATTTAAA 
AATCTCATTG CCAATTTTGT GCATCGAAAC AGCAAAAAAG GAATGCAAAC ATTTGAAACG 
GAAGACATTT CTCCGTACCT CGCATCTCTT TCCAATTCGT GTGTTCCAAT GCCAGGTCAA 
GAATCTGTCG AGTTTGATAG AGTTGTTTCG ATATCTCGTG TTGCTCGACA AGTCACAATT 
CTGCCCACGA AAACTCGCCC GAAAAAGCTG GGATTTGTCG GATCTGATGG AAAACAAGTT 
GCATTTTTAT TCAAAGGACG CGAAGATCTT CATCTTGATG AACGAGTCAT GCAATTCCTC 
CGATTATGTA ATGTGATGCT TCAACCAGGA AAAGGGAAAC ATCGACAATC GGTTGCCGCG 
TATCAGGCAC ATCATTATGC TGTTATTCCA CTTGGACCAC GTTCTGGACT TATCAAATGG 
GTTGAAGGTG CTACACCAAT GTTCCACATA TATCGTAAAT GGCAGATGAA AGAGAAAGCA 
CTCAAACAAG CGACCAAAAA GAACGGAGAA ACAGTTCCTG AAATCGAAAG ACCCAGCAAC 
ATGTATCACA ATATGATTCG TCTAGCATTC GCAGATCATA AAATCGATTC CTCCATCACC 
TCGGATCGTT CTAAATGGCC AGCAGAAATT TTGGAAGAAG TATTTGAAAG TCTCACAGCA 
AAAACACCAA CAGATTTAAT CAGTCGTGAG CTTTGGATGC GTGCGAATGA TGCAACAACT 
TGGTGGTCGG TTACAAAACG ATATTCAAGA TCACTGGCAG TCATGAGTAT GGTTGGATCA 
GTTTTAGGAC TCGGTGATCG ACATTTGGAC AATTTATTGG TTGATTTGAA GTGGGGTCAT 
GTAGTACATA TTGACTACAA CATTTGCTTT GACAAGGGAA AGAATCTGCG TATCCCCGAA 
ACAGTCCCAT TCCGTTTGAC ACGTAACATG CGTCACGCTC TCGGACCATC TGAAATGTAT 
GGTACCTTCC GTGAATCTTG TGTTCACGTG CTCTCAACTC TTCGTTCTGG TCATCAAGTT 
CTTACAATGC TTCTCGATGC ATTTGTATTC GATCCGCTTG TCGATTGGAC ATCACATGAA 
CATACAGCAA CTTCTGGTGT CTCATTGGCA CTACAATTGG CTGTTTATGG AAGTAATTGG 
AAGACAAAAG CTAAAGAAAG ATTGACCGAT GCGATGGAGT TACTCAATTT GAGAATGTCT 
GAGGTTCAAA CCCTTTGGCT TGCAAATCGT GATGACCTTC TCCACTGGAT GAAACAAGTG 
ACCGAATGTC TTTTGATTGA AAATTCGATG CTTGGAGCAA ATGCCATATA TGCTCAGCAA 
CGTGTGAAAG CTGGAACGGA ACTTCGTGAG GCTGTGACAC GTCATCATGC ACTTGCCAAA 
GAGCTTCGTC CATTGATAAG AGTAATTGGA AAGGAACGAG AAGAGTTTGC AGATTATTTG 
AAGTTTTACA AGCAGGCATT ATTTGATCCA CTTTTGAAAG GACACTCGGC TCTCCGTAAC 
GAACTCGATA TTGATACATG TGTGTACAAT TTCAACATTG TCATGCAAAA TATAGATAAT 
GTGTTTGGAG CACTCGTCAA TCTGTCATTC ACCCCGATTG AGACTATTAC ATCCCGTACT 
TCACAGCAAG AATTCAAACC ACCTCCCGGA CTTGAAAATG TATGGGTTGT GAAACAAGAT 
CAACAGGAGA ATTCTCAAGC GCGTGAAGTT GTCCGACGTG TTGAACGGCG TCTCAACGGA 
TGGCTCGATG GATCGGCAGG ACCAGATAGG AAATTGTCTC CTCGCGAAGA GGCCGACATC 
CTAATCGCTG AAGCTACATC CACACCAAAT TTATCTCAAA TGTACGAAGG ATGGACTGCT 
TGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021053.1
CDS	29..6997
Translation	MITSRNNDIGNLIEQFRQRDTPQKERKAILARIEEILQTTKNVESLCVKWTYLLDNLCWPSLTKHDRNDMKTLAGKVIRLVGVLLFDTESYPEFLIYLGTLYQSVTKKSEETRADIVFSVYFIVGVISQKTENRLIATDTENVEKSLDWIIKVLPNSSISVYNHCLKGFVLVANTFPNVYAAMFESTLRAILTNLPDFNSHEKNFELLIDTVMRFSDQLNEKPHLAEEMVRIIRPDIKKNGLGNMRELKKRMKLTMALVKMAKSQKMLEETNQMISEMSIELEENGGKWSSASLITIVCDVFNELLILGKDDVKLQKGVEESLCNVLKDLNLSNQSTMEKQAFFNSLAKIVKQLPAESQVKTRVHQIVFNTETGLFTPKNRDNRMFGHNMIYKDLINLVSVLLTPTSLNHLQATYTDLRKIMIDSMSRLKQSEDTPYSDNIRWNESILLLFFSSLQSISCAKSSLIVMMGIRPSIFEFFSSELPLTEYWLASNHPEVYHLFITIFVGHLKAHDFYIVQSDYIVRGDSIGQSIGQTKRDYARKQVVALQKIINNFGDKLWKKTRLMISSWLHSLIATACEHQIGSDSFSQREWVRLRNTVIHQSVLTWNNECVNQALTILSTATKWSELTSDIHRDIADKTKKAKWKEATTIWESGDCNTYIRQSMSTVYQMSQERQQKTITSTSFGAEEFIIITNFLLKQATPTTFKKGQNSWMDEVLETFTQGCRTLEKPDSYVPETFIEKWDWIINQTANFCIVNKMKTPLGKPMQTFAAFENEIKRLAKEVIVRKNSDKKLNKSSTEDPNQSPPLKYSVQWLRVHLLLKLIVVLEKLMNSAIHGGSSVFNLTEIPVSSRQFFTVNAASCEVWLNRVYYPALLVAYFNGYYGLVIRFGSNALSHFARQKDGDNDKKIVNGVCTASLMSLSMAVLGEPMEIVGLRRKVREEFGTDMGQSLMEALGEMANARYETALVALEAVLVTDAATNETLKMIIQLAMVDILNRIRLPQATDYYKVVLFGEESNDSTITEDFRSVELLTKFEKLNNTVNEKRQVVDWSARERFQFVESAFSQTMRRTELLDIQKDFSAMGALALSADSSCKLYSDISSTSLIIANLVNKMTGVSQWKNKLTDTEIFDRNEEGNDGDKLAICRKLMHWGRHTKSNRGQSCAAHSEIIRLSRKTSNCELAFFHINSAIRGEKLAAWQRLEVERQRLKLVKTQNLDVRIREMNEVFGSLAEVFTTSCQLKSDFQMVDGMIKEKMISEGYNEDIAKREEHMSRASIQLADFFQSLPELENVLAPNLFPTIIWSELQRRSDSLSAGYHGIVGSLFHLASEMCPSLAKAHLKMARWAYEIAKIKNFPAENLSFYKFGKDETENEELLKSLEATSLVNLEKLVRAAISDDLRANNILAPNSHFMHIWKMVRDHRTKFLSIAVTSYFQFIQNMSGDCDNLPYSKKEETTLATLRILELLVKHGDVLIDVINDGLNKTNVHIWKEILPQLFARLSHPSEHIRKTLVDLISKICTAAPHAVVFQVVSGAASSSTDGEELEEQQNDDRNRVRACCEKLETNMSQSYPNLVKDVRQFVAELERINLLNEEKWSVVMGTMEHEMEKRLSLIRTENAKTESALHLTASVKNDIIVKRTQLLTRQIFDVLDELYQQTVIEPPKSKNEEEFVTAFAEVLTNAFQESRISRTTSPEKSWIPFKNLIANFVHRNSKKGMQTFETEDISPYLASLSNSCVPMPGQESVEFDRVVSISRVARQVTILPTKTRPKKLGFVGSDGKQVAFLFKGREDLHLDERVMQFLRLCNVMLQPGKGKHRQSVAAYQAHHYAVIPLGPRSGLIKWVEGATPMFHIYRKWQMKEKALKQATKKNGETVPEIERPSNMYHNMIRLAFADHKIDSSITSDRSKWPAEILEEVFESLTAKTPTDLISRELWMRANDATTWWSVTKRYSRSLAVMSMVGSVLGLGDRHLDNLLVDLKWGHVVHIDYNICFDKGKNLRIPETVPFRLTRNMRHALGPSEMYGTFRESCVHVLSTLRSGHQVLTMLLDAFVFDPLVDWTSHEHTATSGVSLALQLAVYGSNWKTKAKERLTDAMELLNLRMSEVQTLWLANRDDLLHWMKQVTECLLIENSMLGANAIYAQQRVKAGTELREAVTRHHALAKELRPLIRVIGKEREEFADYLKFYKQALFDPLLKGHSALRNELDIDTCVYNFNIVMQNIDNVFGALVNLSFTPIETITSRTSQQEFKPPPGLENVWVVKQDQQENSQAREVVRRVERRLNGWLDGSAGPDRKLSPREEADILIAEATSTPNLSQMYEGWTAWV

Target ORF information:

RefSeq Version	NM_001025882.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase smg-1 (smg-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025882.3

ORF Insert Sequence:

ATGATAACAT CGAGAAACAA TGATATTGGG AACCTGATAG AACAGTTTCG TCAACGTGAC 
ACGCCACAGA AGGAACGGAA AGCGATTCTC GCTAGAATCG AGGAGATTCT TCAAACTACG 
AAAAATGTAG AGAGTCTTTG TGTAAAATGG ACTTATCTTT TGGATAACTT GTGTTGGCCT 
AGTCTCACAA AACACGATCG GAATGACATG AAAACGCTGG CTGGAAAAGT GATACGACTT 
GTTGGAGTTT TACTCTTTGA TACTGAATCC TATCCAGAGT TTCTGATTTA TCTTGGAACA 
TTATATCAAA GTGTTACGAA GAAAAGTGAA GAAACACGTG CGGATATCGT GTTTTCAGTG 
TATTTCATCG TTGGAGTCAT CAGTCAAAAA ACGGAGAATC GTCTAATTGC TACCGACACT 
GAAAATGTCG AAAAATCATT AGACTGGATA ATCAAAGTGC TTCCGAATTC ATCAATATCA 
GTTTATAACC ACTGTTTGAA AGGATTTGTA CTTGTTGCAA ACACATTTCC AAATGTTTAT 
GCTGCAATGT TTGAGTCAAC CCTCCGAGCA ATTCTGACCA ACCTTCCCGA TTTCAACAGT 
CATGAAAAGA ATTTTGAGCT TCTCATTGAT ACAGTGATGC GATTTTCTGA TCAACTTAAT 
GAGAAGCCTC ATTTGGCAGA AGAAATGGTT CGAATTATTC GACCGGATAT CAAAAAGAAT 
GGATTGGGAA ATATGAGAGA ATTGAAGAAA CGAATGAAAC TGACAATGGC ACTCGTGAAA 
ATGGCGAAAA GTCAAAAAAT GCTTGAGGAG ACTAATCAGA TGATTTCTGA AATGTCTATC 
GAACTCGAGG AAAACGGAGG AAAATGGTCA TCAGCTTCAC TTATAACTAT CGTTTGTGAT 
GTTTTCAATG AGCTCTTGAT ATTGGGAAAA GATGATGTAA AATTGCAAAA AGGAGTTGAA 
GAATCATTGT GCAATGTTCT GAAAGACTTG AATCTTTCTA ATCAGAGTAC TATGGAGAAA 
CAAGCATTTT TCAATTCTCT TGCAAAGATT GTCAAACAAC TTCCAGCAGA GTCACAAGTC 
AAAACTAGAG TTCATCAGAT TGTTTTCAAC ACGGAAACTG GACTTTTCAC ACCCAAAAAC 
CGCGATAATC GAATGTTTGG ACATAATATG ATATACAAGG ATCTTATCAA TCTTGTCTCT 
GTGCTTCTTA CGCCTACATC ACTCAATCAT CTACAAGCGA CATATACGGA TTTGAGGAAA 
ATAATGATAG ATAGCATGTC ACGATTGAAG CAAAGTGAAG ATACTCCGTA TAGTGATAAT 
ATTCGATGGA ATGAATCAAT CCTTTTGCTT TTCTTTTCGT CACTTCAAAG TATATCATGT 
GCCAAGAGTT CTCTTATTGT GATGATGGGA ATCCGTCCGT CAATTTTTGA ATTCTTCTCA 
TCGGAACTTC CACTCACAGA ATATTGGCTT GCTTCAAATC ATCCAGAAGT CTATCACCTA 
TTTATCACAA TATTTGTTGG ACATTTGAAA GCTCACGACT TCTACATTGT TCAATCTGAT 
TATATTGTTC GTGGTGATAG CATTGGACAA AGTATAGGAC AAACGAAACG AGATTATGCA 
CGGAAACAAG TTGTTGCTCT ACAGAAAATT ATCAACAATT TTGGAGACAA ATTGTGGAAG 
AAGACTAGAC TGATGATATC TTCATGGCTT CACTCTCTCA TTGCAACTGC TTGTGAACAT 
CAAATCGGAA GTGATAGTTT CAGTCAACGA GAATGGGTTC GATTAAGGAA TACTGTCATT 
CATCAGTCGG TGCTCACATG GAATAATGAA TGTGTAAATC AAGCATTGAC CATCCTCTCC 
ACTGCTACAA AATGGTCAGA ACTGACGTCA GACATCCATC GAGATATTGC GGATAAAACG 
AAAAAAGCAA AGTGGAAGGA GGCAACGACA ATTTGGGAAT CTGGAGATTG TAATACTTAT 
ATCAGGCAAT CCATGTCTAC AGTCTATCAA ATGTCACAGG AACGACAACA GAAGACAATT 
ACATCGACAA GTTTTGGAGC CGAAGAGTTT ATAATAATTA CGAATTTCTT GTTGAAACAG 
GCAACACCAA CTACTTTCAA AAAAGGTCAA AACTCATGGA TGGATGAAGT ACTGGAAACA 
TTCACACAAG GATGTCGTAC GCTTGAAAAA CCCGATTCAT ACGTTCCAGA GACATTTATC 
GAAAAATGGG ATTGGATTAT TAATCAGACT GCAAACTTTT GCATTGTGAA TAAAATGAAG 
ACACCATTAG GAAAACCAAT GCAGACTTTT GCAGCTTTTG AGAATGAAAT TAAACGACTT 
GCGAAAGAAG TGATTGTTCG AAAGAATAGT GATAAGAAGT TGAATAAATC ATCTACAGAA 
GATCCAAATC AATCTCCACC ACTCAAATAT TCCGTTCAAT GGCTTCGTGT TCATTTACTT 
CTAAAATTGA TTGTAGTTTT GGAAAAACTT ATGAACTCAG CCATTCATGG TGGTTCTTCC 
GTTTTCAATT TGACTGAAAT TCCAGTGTCC TCTCGCCAAT TTTTCACAGT GAACGCAGCA 
AGTTGTGAGG TTTGGCTGAA TCGAGTATAC TATCCTGCTC TTCTTGTCGC ATATTTCAAT 
GGATATTATG GACTTGTTAT TCGATTTGGT TCAAATGCTC TTAGCCATTT CGCGAGACAA 
AAAGACGGAG ATAATGATAA AAAGATCGTG AATGGCGTTT GTACAGCAAG TTTAATGTCA 
CTTTCAATGG CAGTTCTCGG TGAACCAATG GAAATTGTTG GATTACGGAG AAAAGTTCGA 
GAAGAATTCG GAACTGACAT GGGACAATCA TTGATGGAGG CACTCGGAGA AATGGCTAAT 
GCTAGATATG AGACAGCTCT GGTTGCCTTG GAAGCAGTTC TTGTGACAGA TGCAGCAACA 
AATGAAACAT TGAAAATGAT CATTCAACTA GCAATGGTTG ACATTTTGAA CCGCATACGC 
CTCCCACAGG CTACCGATTA TTATAAGGTT GTCTTATTTG GTGAAGAATC AAATGATTCC 
ACAATTACTG AAGACTTTCG ATCAGTTGAG TTACTCACGA AATTCGAGAA GCTCAACAAT 
ACAGTGAACG AAAAGCGTCA AGTTGTTGAT TGGAGTGCTC GAGAACGTTT CCAATTTGTT 
GAATCCGCAT TTTCACAGAC TATGAGAAGA ACCGAACTTC TCGACATTCA AAAAGATTTT 
TCCGCGATGG GAGCACTTGC ATTGAGTGCT GATTCATCGT GTAAATTGTA TTCGGACATT 
TCATCAACAT CTCTCATAAT TGCAAATCTT GTCAATAAAA TGACTGGTGT CTCACAATGG 
AAGAATAAAC TCACTGATAC GGAAATATTC GATAGAAATG AAGAAGGAAA TGATGGTGAT 
AAGTTGGCAA TATGTCGAAA GTTAATGCAT TGGGGACGAC ACACAAAGAG TAATCGCGGA 
CAGTCATGCG CTGCACACAG TGAAATAATT CGTTTATCCC GTAAAACTTC AAATTGTGAA 
CTCGCGTTCT TTCACATAAA TTCAGCTATT CGAGGTGAAA AACTAGCTGC TTGGCAGAGA 
TTAGAAGTGG AAAGACAGCG ACTGAAACTA GTGAAAACTC AAAATTTGGA TGTGCGAATT 
CGTGAAATGA ATGAAGTGTT CGGATCCTTG GCTGAAGTTT TTACGACAAG TTGTCAACTG 
AAATCAGACT TTCAAATGGT TGATGGAATG ATTAAAGAAA AAATGATCTC CGAAGGATAT 
AATGAAGATA TTGCAAAGCG TGAGGAGCAC ATGAGTCGAG CCAGTATTCA ACTTGCAGAC 
TTTTTCCAAT CACTTCCAGA ACTTGAAAAT GTACTTGCGC CCAACCTGTT CCCGACAATT 
ATTTGGAGTG AACTACAACG TCGATCAGAT AGTTTATCTG CTGGATATCA TGGAATAGTT 
GGATCTCTCT TTCATTTGGC ATCTGAAATG TGTCCATCTC TCGCTAAAGC TCATCTCAAA 
ATGGCCAGAT GGGCATATGA AATCGCTAAA ATCAAGAATT TCCCTGCTGA AAATTTAAGT 
TTCTACAAAT TTGGAAAAGA CGAAACTGAA AACGAGGAGC TATTGAAGAG CCTGGAAGCG 
ACGAGTCTTG TTAATTTGGA AAAGCTTGTT CGAGCTGCAA TTTCTGATGA TTTGAGAGCA 
AACAACATTC TGGCACCCAA TAGTCACTTT ATGCACATTT GGAAAATGGT TCGGGATCAT 
CGCACGAAAT TCTTGTCAAT TGCTGTGACT TCATATTTCC AATTTATTCA GAATATGTCC 
GGTGATTGTG ATAATCTACC GTACTCGAAA AAAGAGGAGA CCACTTTGGC GACATTGAGA 
ATTCTAGAAC TTCTTGTCAA ACATGGCGAT GTTCTCATTG ATGTAATAAA TGATGGACTA 
AATAAGACTA ATGTACATAT TTGGAAAGAA ATTCTTCCTC AATTGTTTGC TCGCTTGTCC 
CATCCATCTG AACATATTCG AAAAACTCTT GTTGATCTAA TTTCAAAAAT CTGTACAGCT 
GCACCACATG CAGTCGTTTT TCAAGTTGTT TCTGGTGCTG CATCATCTTC TACAGACGGT 
GAAGAACTTG AAGAGCAGCA GAATGATGAT CGTAACCGAG TACGTGCATG CTGTGAGAAA 
TTGGAGACAA ATATGTCACA ATCATATCCG AATCTTGTGA AAGATGTACG GCAGTTTGTA 
GCAGAACTTG AACGAATTAA TCTTTTGAAT GAAGAGAAAT GGTCTGTTGT CATGGGAACA 
ATGGAACATG AAATGGAAAA ACGATTATCA TTGATTAGAA CTGAAAATGC GAAAACTGAA 
TCGGCTCTTC ATTTAACAGC TTCAGTCAAA AATGATATTA TTGTGAAAAG AACCCAATTG 
TTGACTAGAC AGATATTTGA TGTACTCGAT GAGCTCTACC AACAAACAGT CATTGAGCCT 
CCAAAGAGCA AGAATGAAGA AGAGTTTGTA ACAGCTTTTG CCGAAGTACT CACAAACGCT 
TTTCAAGAGT CCAGAATATC ACGTACCACT TCTCCGGAAA AGTCATGGAT TCCATTTAAA 
AATCTCATTG CCAATTTTGT GCATCGAAAC AGCAAAAAAG GAATGCAAAC ATTTGAAACG 
GAAGACATTT CTCCGTACCT CGCATCTCTT TCCAATTCGT GTGTTCCAAT GCCAGGTCAA 
GAATCTGTCG AGTTTGATAG AGTTGTTTCG ATATCTCGTG TTGCTCGACA AGTCACAATT 
CTGCCCACGA AAACTCGCCC GAAAAAGCTG GGATTTGTCG GATCTGATGG AAAACAAGTT 
GCATTTTTAT TCAAAGGACG CGAAGATCTT CATCTTGATG AACGAGTCAT GCAATTCCTC 
CGATTATGTA ATGTGATGCT TCAACCAGGA AAAGGGAAAC ATCGACAATC GGTTGCCGCG 
TATCAGGCAC ATCATTATGC TGTTATTCCA CTTGGACCAC GTTCTGGACT TATCAAATGG 
GTTGAAGGTG CTACACCAAT GTTCCACATA TATCGTAAAT GGCAGATGAA AGAGAAAGCA 
CTCAAACAAG CGACCAAAAA GAACGGAGAA ACAGTTCCTG AAATCGAAAG ACCCAGCAAC 
ATGTATCACA ATATGATTCG TCTAGCATTC GCAGATCATA AAATCGATTC CTCCATCACC 
TCGGATCGTT CTAAATGGCC AGCAGAAATT TTGGAAGAAG TATTTGAAAG TCTCACAGCA 
AAAACACCAA CAGATTTAAT CAGTCGTGAG CTTTGGATGC GTGCGAATGA TGCAACAACT 
TGGTGGTCGG TTACAAAACG ATATTCAAGA TCACTGGCAG TCATGAGTAT GGTTGGATCA 
GTTTTAGGAC TCGGTGATCG ACATTTGGAC AATTTATTGG TTGATTTGAA GTGGGGTCAT 
GTAGTACATA TTGACTACAA CATTTGCTTT GACAAGGGAA AGAATCTGCG TATCCCCGAA 
ACAGTCCCAT TCCGTTTGAC ACGTAACATG CGTCACGCTC TCGGACCATC TGAAATGTAT 
GGTACCTTCC GTGAATCTTG TGTTCACGTG CTCTCAACTC TTCGTTCTGG TCATCAAGTT 
CTTACAATGC TTCTCGATGC ATTTGTATTC GATCCGCTTG TCGATTGGAC ATCACATGAA 
CATACAGCAA CTTCTGGTGT CTCATTGGCA CTACAATTGG CTGTTTATGG AAGTAATTGG 
AAGACAAAAG CTAAAGAAAG ATTGACCGAT GCGATGGAGT TACTCAATTT GAGAATGTCT 
GAGGTTCAAA CCCTTTGGCT TGCAAATCGT GATGACCTTC TCCACTGGAT GAAACAAGTG 
ACCGAATGTC TTTTGATTGA AAATTCGATG CTTGGAGCAA ATGCCATATA TGCTCAGCAA 
CGTGTGAAAG CTGGAACGGA ACTTCGTGAG GCTGTGACAC GTCATCATGC ACTTGCCAAA 
GAGCTTCGTC CATTGATAAG AGTAATTGGA AAGGAACGAG AAGAGTTTGC AGATTATTTG 
AAGTTTTACA AGCAGGCATT ATTTGATCCA CTTTTGAAAG GACACTCGGC TCTCCGTAAC 
GAACTCGATA TTGATACATG TGTGTACAAT TTCAACATTG TCATGCAAAA TATAGATAAT 
GTGTTTGGAG CACTCGTCAA TCTGTCATTC ACCCCGATTG AGACTATTAC ATCCCGTACT 
TCACAGCAAG AATTCAAACC ACCTCCCGGA CTTGAAAATG TATGGGTTGT GAAACAAGAT 
CAACAGGAGA ATTCTCAAGC GCGTGAAGTT GTCCGACGTG TTGAACGGCG TCTCAACGGA 
TGGCTCGATG GATCGGCAGG ACCAGATAGG AAATTGTCTC CTCGCGAAGA GGCCGACATC 
CTAATCGCTG AAGCTACATC CACACCAAAT TTATCTCAAA TGTACGAAGG ATGGACTGCT 
TGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smg-1 ( NM_001025883.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021054.1 Caenorhabditis elegans Serine/threonine-protein kinase smg-1 (smg-1), partial mRNA.

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CloneID	OCa336707
Clone ID Related Accession (Same CDS sequence)	NM_001025883.3
Accession Version	NM_001025883.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase smg-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_001025883.2. COMPLETENESS: incomplete on both ends.

ATGATAACAT CGAGAAACAA TGATATTGGG AACCTGATAG AACAGTTTCG TCAACGTGAC 
ACGCCACAGA AGGAACGGAA AGCGATTCTC GCTAGAATCG AGGAGATTCT TCAAACTACG 
AAAAATGTAG AGAGTCTTTG TGTAAAATGG ACTTATCTTT TGGATAACTT GTGTTGGCCT 
AGTCTCACAA AACACGATCG GAATGACATG AAAACGCTGG CTGGAAAAGT GATACGACTT 
GTTGGAGTTT TACTCTTTGA TACTGAATCC TATCCAGAGT TTCTGATTTA TCTTGGAACA 
TTATATCAAA GTGTTACGAA GAAAAGTGAA GAAACACGTG CGGATATCGT GTTTTCAGTG 
TATTTCATCG TTGGAGTCAT CAGTCAAAAA ACGGAGAATC GTCTAATTGC TACCGACACT 
GAAAATGTCG AAAAATCATT AGACTGGATA ATCAAAGTGC TTCCGAATTC ATCAATATCA 
GTTTATAACC ACTGTTTGAA AGGATTTGTA CTTGTTGCAA ACACATTTCC AAATGTTTAT 
GCTGCAATGT TTGAGTCAAC CCTCCGAGCA ATTCTGACCA ACCTTCCCGA TTTCAACAGT 
CATGAAAAGA ATTTTGAGCT TCTCATTGAT ACAGTGATGC GATTTTCTGA TCAACTTAAT 
GAGAAGCCTC ATTTGGCAGA AGAAATGGTT CGAATTATTC GACCGGATAT CAAAAAGAAT 
GGATTGGGAA ATATGAGAGA ATTGAAGAAA CGAATGAAAC TGACAATGGC ACTCGTGAAA 
ATGGCGAAAA GTCAAAAAAT GCTTGAGGAG ACTAATCAGA TGATTTCTGA AATGTCTATC 
GAACTCGAGG AAAACGGAGG AAAATGGTCA TCAGCTTCAC TTATAACTAT CGTTTGTGAT 
GTTTTCAATG AGCTCTTGAT ATTGGGAAAA GATGATGTAA AATTGCAAAA AGGAGTTGAA 
GAATCATTGT GCAATGTTCT GAAAGACTTG AATCTTTCTA ATCAGAGTAC TATGGAGAAA 
CAAGCATTTT TCAATTCTCT TGCAAAGATT GTCAAACAAC TTCCAGCAGA GTCACAAGTC 
AAAACTAGAG TTCATCAGAT TGTTTTCAAC ACGGAAACTG GACTTTTCAC ACCCAAAAAC 
CGCGATAATC GAATGTTTGG ACATAATATG ATATACAAGG ATCTTATCAA TCTTGTCTCT 
GTGCTTCTTA CGCCTACATC ACTCAATCAT CTACAAGCGA CATATACGGA TTTGAGGAAA 
ATAATGATAG ATAGCATGTC ACGATTGAAG CAAAGTGAAG ATACTCCGTA TAGTGATAAT 
ATTCGATGGA ATGAATCAAT CCTTTTGCTT TTCTTTTCGT CACTTCAAAG TATATCATGT 
GCCAAGAGTT CTCTTATTGT GATGATGGGA ATCCGTCCGT CAATTTTTGA ATTCTTCTCA 
TCGGAACTTC CACTCACAGA ATATTGGCTT GCTTCAAATC ATCCAGAAGT CTATCACCTA 
TTTATCACAA TATTTGTTGG ACATTTGAAA GCTCACGACT TCTACATTGT TCAATCTGAT 
TATATTGTTC GTGGTGATAG CATTGGACAA AGTATAGGAC AAACGAAACG AGATTATGCA 
CGGAAACAAG TTGTTGCTCT ACAGAAAATT ATCAACAATT TTGGAGACAA ATTGTGGAAG 
AAGACTAGAC TGATGATATC TTCATGGCTT CACTCTCTCA TTGCAACTGC TTGTGAACAT 
CAAATCGGAA GTGATAGTTT CAGTCAACGA GAATGGGTTC GATTAAGGAA TACTGTCATT 
CATCAGTCGG TGCTCACATG GAATAATGAA TGTGTAAATC AAGCATTGAC CATCCTCTCC 
ACTGCTACAA AATGGTCAGA ACTGACGTCA GACATCCATC GAGATATTGC GGATAAAACG 
AAAAAAGCAA AGTGGAAGGA GGCAACGACA ATTTGGGAAT CTGGAGATTG TAATACTTAT 
ATCAGGCAAT CCATGTCTAC AGTCTATCAA ATGTCACAGG AACGACAACA GAAGACAATT 
ACATCGACAA GTTTTGGAGC CGAAGAGTTT ATAATAATTA CGAATTTCTT GTTGAAACAG 
GCAACACCAA CTACTTTCAA AAAAGGTCAA AACTCATGGA TGGATGAAGT ACTGGAAACA 
TTCACACAAG GATGTCGTAC GCTTGAAAAA CCCGATTCAT ACGTTCCAGA GACATTTATC 
GAAAAATGGG ATTGGATTAT TAATCAGACT GCAAACTTTT GCATTGTGAA TAAAATGAAG 
ACACCATTAG GAAAACCAAT GCAGACTTTT GCAGCTTTTG AGAATGAAAT TAAACGACTT 
GCGAAAGAAG TGATTGTTCG AAAGAATAGT GATAAGAAGT TGAATAAATC ATCTACAGAA 
GATCCAAATC AATCTCCACC ACTCAAATAT TCCGTTCAAT GGCTTCGTGT TCATTTACTT 
CTAAAATTGA TTGTAGTTTT GGAAAAACTT ATGAACTCAG CCATTCATGG TGGTTCTTCC 
GTTTTCAATT TGACTGAAAT TCCAGTGTCC TCTCGCCAAT TTTTCACAGT GAACGCAGCA 
AGTTGTGAGG TTTGGCTGAA TCGAGTATAC TATCCTGCTC TTCTTGTCGC ATATTTCAAT 
GGATATTATG GACTTGTTAT TCGATTTGGT TCAAATGCTC TTAGCCATTT CGCGAGACAA 
AAAGACGGAG ATAATGATAA AAAGATCGTG AATGGCGTTT GTACAGCAAG TTTAATGTCA 
CTTTCAATGG CAGTTCTCGG TGAACCAATG GAAATTGTTG GATTACGGAG AAAAGTTCGA 
GAAGAATTCG GAACTGACAT GGGACAATCA TTGATGGAGG CACTCGGAGA AATGGCTAAT 
GCTAGATATG AGACAGCTCT GGTTGCCTTG GAAGCAGTTC TTGTGACAGA TGCAGCAACA 
AATGAAACAT TGAAAATGAT CATTCAACTA GCAATGGTTG ACATTTTGAA CCGCATACGC 
CTCCCACAGG CTACCGATTA TTATAAGGTT GTCTTATTTG GTGAAGAATC AAATGATTCC 
ACAATTACTG AAGACTTTCG ATCAGTTGAG TTACTCACGA AATTCGAGAA GCTCAACAAT 
ACAGTGAACG AAAAGCGTCA AGTTGTTGAT TGGAGTGCTC GAGAACGTTT CCAATTTGTT 
GAATCCGCAT TTTCACAGAC TATGAGAAGA ACCGAACTTC TCGACATTCA AAAAGATTTT 
TCCGCGATGG GAGCACTTGC ATTGAGTGCT GATTCATCGT GTAAATTGTA TTCGGACATT 
TCATCAACAT CTCTCATAAT TGCAAATCTT GTCAATAAAA TGACTGGTGT CTCACAATGG 
AAGAATAAAC TCACTGATAC GGAAATATTC GATAGAAATG AAGAAGGAAA TGATGGTGAT 
AAGTTGGCAA TATGTCGAAA GTTAATGCAT TGGGGACGAC ACACAAAGAG TAATCGCGGA 
CAGTCATGCG CTGCACACAG TGAAATAATT CGTTTATCCC GTAAAACTTC AAATTGTGAA 
CTCGCGTTCT TTCACATAAA TTCAGCTATT CGAGGTGAAA AACTAGCTGC TTGGCAGAGA 
TTAGAAGTGG AAAGACAGCG ACTGAAACTA GTGAAAACTC AAAATTTGGA TGTGCGAATT 
CGTGAAATGA ATGAAGTGTT CGGATCCTTG GCTGAAGTTT TTACGACAAG TTGTCAACTG 
AAATCAGACT TTCAAATGGT TGATGGAATG ATTAAAGAAA AAATGATCTC CGAAGGATAT 
AATGAAGATA TTGCAAAGCG TGAGGAGCAC ATGAGTCGAG CCAGTATTCA ACTTGCAGAC 
TTTTTCCAAT CACTTCCAGA ACTTGAAAAT GTACTTGCGC CCAACCTGTT CCCGACAATT 
ATTTGGAGTG AACTACAACG TCGATCAGAT AGTTTATCTG CTGGATATCA TGGAATAGTT 
GGATCTCTCT TTCATTTGGC ATCTGAAATG TGTCCATCTC TCGCTAAAGC TCATCTCAAA 
ATGGCCAGAT GGGCATATGA AATCGCTAAA ATCAAGAATT TCCCTGCTGA AAATTTAAGT 
TTCTACAAAT TTGGAAAAGA CGAAACTGAA AACGAGGAGC TATTGAAGAG CCTGGAAGCG 
ACGAGTCTTG TTAATTTGGA AAAGCTTGTT CGAGCTGCAA TTTCTGATGA TTTGAGAGCA 
AACAACATTC TGGCACCCAA TAGTCACTTT ATGCACATTT GGAAAATGGT TCGGGATCAT 
CGCACGAAAT TCTTGTCAAT TGCTGTGACT TCATATTTCC AATTTATTCA GAATATGTCC 
GGTGATTGTG ATAATCTACC GTACTCGAAA AAAGAGGAGA CCACTTTGGC GACATTGAGA 
ATTCTAGAAC TTCTTGTCAA ACATGGCGAT GTTCTCATTG ATGTAATAAA TGATGGACTA 
AATAAGACTA ATGTACATAT TTGGAAAGAA ATTCTTCCTC AATTGTTTGC TCGCTTGTCC 
CATCCATCTG AACATATTCG AAAAACTCTT GTTGATCTAA TTTCAAAAAT CTGTACAGCT 
GCACCACATG CAGTCGTTTT TCAAGTTGTT TCTGGTGCTG CATCATCTTC TACAGACGGT 
GAAGAACTTG AAGAGCAGCA GAATGATGAT CGTAACCGAG TACGTGCATG CTGTGAGAAA 
TTGGAGACAA ATATGTCACA ATCATATCCG AATCTTGTGA AAGATGTACG GCAGTTTGTA 
GCAGAACTTG AACGAATTAA TCTTTTGAAT GAAGAGAAAT GGTCTGTTGT CATGGGAACA 
ATGGAACATG AAATGGAAAA ACGATTATCA TTGATTAGAA CTGAAAATGC GAAAACTGAA 
TCGGCTCTTC ATTTAACAGC TTCAGTCAAA AATGATATTA TTGTGAAAAG AACCCAATTG 
TTGACTAGAC AGATATTTGA TGTACTCGAT GAGCTCTACC AACAAACAGT CATTGAGCCT 
CCAAAGAGCA AGAATGAAGA AGAGTTTGTA ACAGCTTTTG CCGAAGTACT CACAAACGCT 
TTTCAAGAGT CCAGAATATC ACGTACCACT TCTCCGGAAA AGTCATGGAT TCCATTTAAA 
AATCTCATTG CCAATTTTGT GCATCGAAAC AGCAAAAAAG GAATGCAAAC ATTTGAAACG 
GAAGACATTT CTCCGTACCT CGCATCTCTT TCCAATTCGT GTGTTCCAAT GCCAGGTCAA 
GAATCTGTCG AGTTTGATAG AGTTGTTTCG ATATCTCGTG TTGCTCGACA AGTCACAATT 
CTGCCCACGA AAACTCGCCC GAAAAAGCTG GGATTTGTCG GATCTGATGG AAAACAAGTT 
GCATTTTTAT TCAAAGGACG CGAAGATCTT CATCTTGATG AACGAGTCAT GCAATTCCTC 
CGATTATGTA ATGTGATGCT TCAACCAGGA AAAGGGAAAC ATCGACAATC GGTTGCCGCG 
TATCAGGCAC ATCATTATGC TGTTATTCCA CTTGGACCAC GTTCTGGACT TATCAAATGG 
GTTGAAGGTG CTACACCAAT GTTCCACATA TATCGTAAAT GGCAGATGAA AGAGCTTCGT 
CCATTGATAA GAGTAATTGG AAAGGAACGA GAAGAGTTTG CAGATTATTT GAAGTTTTAC 
AAGCAGGCAT TATTTGATCC ACTTTTGAAA GGACACTCGG CTCTCCGTAA CGAACTCGAT 
ATTGATACAT GTGTGTACAA TTTCAACATT GTCATGCAAA ATATAGATAA TGTGTTTGGA 
GCACTCGTCA ATCTGTCATT CACCCCGATT GAGACTATTA CATCCCGTAC TTCACAGCAA 
GAATTCAAAC CACCTCCCGG ACTTGAAAAT GTATGGGTTG TGAAACAAGA TCAACAGGAG 
AATTCTCAAG CGCGTGAAGT TGTCCGACGT GTTGAACGGC GTCTCAACGG ATGGCTCGAT 
GGATCGGCAG GACCAGATAG GAAATTGTCT CCTCGCGAAG AGGCCGACAT CCTAATCGCT 
GAAGCTACAT CCACACCAAA TTTATCTCAA ATGTACGAAG GATGGACTGC TTGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021054.1
CDS	1..6060
Translation	MITSRNNDIGNLIEQFRQRDTPQKERKAILARIEEILQTTKNVESLCVKWTYLLDNLCWPSLTKHDRNDMKTLAGKVIRLVGVLLFDTESYPEFLIYLGTLYQSVTKKSEETRADIVFSVYFIVGVISQKTENRLIATDTENVEKSLDWIIKVLPNSSISVYNHCLKGFVLVANTFPNVYAAMFESTLRAILTNLPDFNSHEKNFELLIDTVMRFSDQLNEKPHLAEEMVRIIRPDIKKNGLGNMRELKKRMKLTMALVKMAKSQKMLEETNQMISEMSIELEENGGKWSSASLITIVCDVFNELLILGKDDVKLQKGVEESLCNVLKDLNLSNQSTMEKQAFFNSLAKIVKQLPAESQVKTRVHQIVFNTETGLFTPKNRDNRMFGHNMIYKDLINLVSVLLTPTSLNHLQATYTDLRKIMIDSMSRLKQSEDTPYSDNIRWNESILLLFFSSLQSISCAKSSLIVMMGIRPSIFEFFSSELPLTEYWLASNHPEVYHLFITIFVGHLKAHDFYIVQSDYIVRGDSIGQSIGQTKRDYARKQVVALQKIINNFGDKLWKKTRLMISSWLHSLIATACEHQIGSDSFSQREWVRLRNTVIHQSVLTWNNECVNQALTILSTATKWSELTSDIHRDIADKTKKAKWKEATTIWESGDCNTYIRQSMSTVYQMSQERQQKTITSTSFGAEEFIIITNFLLKQATPTTFKKGQNSWMDEVLETFTQGCRTLEKPDSYVPETFIEKWDWIINQTANFCIVNKMKTPLGKPMQTFAAFENEIKRLAKEVIVRKNSDKKLNKSSTEDPNQSPPLKYSVQWLRVHLLLKLIVVLEKLMNSAIHGGSSVFNLTEIPVSSRQFFTVNAASCEVWLNRVYYPALLVAYFNGYYGLVIRFGSNALSHFARQKDGDNDKKIVNGVCTASLMSLSMAVLGEPMEIVGLRRKVREEFGTDMGQSLMEALGEMANARYETALVALEAVLVTDAATNETLKMIIQLAMVDILNRIRLPQATDYYKVVLFGEESNDSTITEDFRSVELLTKFEKLNNTVNEKRQVVDWSARERFQFVESAFSQTMRRTELLDIQKDFSAMGALALSADSSCKLYSDISSTSLIIANLVNKMTGVSQWKNKLTDTEIFDRNEEGNDGDKLAICRKLMHWGRHTKSNRGQSCAAHSEIIRLSRKTSNCELAFFHINSAIRGEKLAAWQRLEVERQRLKLVKTQNLDVRIREMNEVFGSLAEVFTTSCQLKSDFQMVDGMIKEKMISEGYNEDIAKREEHMSRASIQLADFFQSLPELENVLAPNLFPTIIWSELQRRSDSLSAGYHGIVGSLFHLASEMCPSLAKAHLKMARWAYEIAKIKNFPAENLSFYKFGKDETENEELLKSLEATSLVNLEKLVRAAISDDLRANNILAPNSHFMHIWKMVRDHRTKFLSIAVTSYFQFIQNMSGDCDNLPYSKKEETTLATLRILELLVKHGDVLIDVINDGLNKTNVHIWKEILPQLFARLSHPSEHIRKTLVDLISKICTAAPHAVVFQVVSGAASSSTDGEELEEQQNDDRNRVRACCEKLETNMSQSYPNLVKDVRQFVAELERINLLNEEKWSVVMGTMEHEMEKRLSLIRTENAKTESALHLTASVKNDIIVKRTQLLTRQIFDVLDELYQQTVIEPPKSKNEEEFVTAFAEVLTNAFQESRISRTTSPEKSWIPFKNLIANFVHRNSKKGMQTFETEDISPYLASLSNSCVPMPGQESVEFDRVVSISRVARQVTILPTKTRPKKLGFVGSDGKQVAFLFKGREDLHLDERVMQFLRLCNVMLQPGKGKHRQSVAAYQAHHYAVIPLGPRSGLIKWVEGATPMFHIYRKWQMKELRPLIRVIGKEREEFADYLKFYKQALFDPLLKGHSALRNELDIDTCVYNFNIVMQNIDNVFGALVNLSFTPIETITSRTSQQEFKPPPGLENVWVVKQDQQENSQAREVVRRVERRLNGWLDGSAGPDRKLSPREEADILIAEATSTPNLSQMYEGWTAWV

Target ORF information:

RefSeq Version	NM_001025883.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase smg-1 (smg-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025883.3

ORF Insert Sequence:

ATGATAACAT CGAGAAACAA TGATATTGGG AACCTGATAG AACAGTTTCG TCAACGTGAC 
ACGCCACAGA AGGAACGGAA AGCGATTCTC GCTAGAATCG AGGAGATTCT TCAAACTACG 
AAAAATGTAG AGAGTCTTTG TGTAAAATGG ACTTATCTTT TGGATAACTT GTGTTGGCCT 
AGTCTCACAA AACACGATCG GAATGACATG AAAACGCTGG CTGGAAAAGT GATACGACTT 
GTTGGAGTTT TACTCTTTGA TACTGAATCC TATCCAGAGT TTCTGATTTA TCTTGGAACA 
TTATATCAAA GTGTTACGAA GAAAAGTGAA GAAACACGTG CGGATATCGT GTTTTCAGTG 
TATTTCATCG TTGGAGTCAT CAGTCAAAAA ACGGAGAATC GTCTAATTGC TACCGACACT 
GAAAATGTCG AAAAATCATT AGACTGGATA ATCAAAGTGC TTCCGAATTC ATCAATATCA 
GTTTATAACC ACTGTTTGAA AGGATTTGTA CTTGTTGCAA ACACATTTCC AAATGTTTAT 
GCTGCAATGT TTGAGTCAAC CCTCCGAGCA ATTCTGACCA ACCTTCCCGA TTTCAACAGT 
CATGAAAAGA ATTTTGAGCT TCTCATTGAT ACAGTGATGC GATTTTCTGA TCAACTTAAT 
GAGAAGCCTC ATTTGGCAGA AGAAATGGTT CGAATTATTC GACCGGATAT CAAAAAGAAT 
GGATTGGGAA ATATGAGAGA ATTGAAGAAA CGAATGAAAC TGACAATGGC ACTCGTGAAA 
ATGGCGAAAA GTCAAAAAAT GCTTGAGGAG ACTAATCAGA TGATTTCTGA AATGTCTATC 
GAACTCGAGG AAAACGGAGG AAAATGGTCA TCAGCTTCAC TTATAACTAT CGTTTGTGAT 
GTTTTCAATG AGCTCTTGAT ATTGGGAAAA GATGATGTAA AATTGCAAAA AGGAGTTGAA 
GAATCATTGT GCAATGTTCT GAAAGACTTG AATCTTTCTA ATCAGAGTAC TATGGAGAAA 
CAAGCATTTT TCAATTCTCT TGCAAAGATT GTCAAACAAC TTCCAGCAGA GTCACAAGTC 
AAAACTAGAG TTCATCAGAT TGTTTTCAAC ACGGAAACTG GACTTTTCAC ACCCAAAAAC 
CGCGATAATC GAATGTTTGG ACATAATATG ATATACAAGG ATCTTATCAA TCTTGTCTCT 
GTGCTTCTTA CGCCTACATC ACTCAATCAT CTACAAGCGA CATATACGGA TTTGAGGAAA 
ATAATGATAG ATAGCATGTC ACGATTGAAG CAAAGTGAAG ATACTCCGTA TAGTGATAAT 
ATTCGATGGA ATGAATCAAT CCTTTTGCTT TTCTTTTCGT CACTTCAAAG TATATCATGT 
GCCAAGAGTT CTCTTATTGT GATGATGGGA ATCCGTCCGT CAATTTTTGA ATTCTTCTCA 
TCGGAACTTC CACTCACAGA ATATTGGCTT GCTTCAAATC ATCCAGAAGT CTATCACCTA 
TTTATCACAA TATTTGTTGG ACATTTGAAA GCTCACGACT TCTACATTGT TCAATCTGAT 
TATATTGTTC GTGGTGATAG CATTGGACAA AGTATAGGAC AAACGAAACG AGATTATGCA 
CGGAAACAAG TTGTTGCTCT ACAGAAAATT ATCAACAATT TTGGAGACAA ATTGTGGAAG 
AAGACTAGAC TGATGATATC TTCATGGCTT CACTCTCTCA TTGCAACTGC TTGTGAACAT 
CAAATCGGAA GTGATAGTTT CAGTCAACGA GAATGGGTTC GATTAAGGAA TACTGTCATT 
CATCAGTCGG TGCTCACATG GAATAATGAA TGTGTAAATC AAGCATTGAC CATCCTCTCC 
ACTGCTACAA AATGGTCAGA ACTGACGTCA GACATCCATC GAGATATTGC GGATAAAACG 
AAAAAAGCAA AGTGGAAGGA GGCAACGACA ATTTGGGAAT CTGGAGATTG TAATACTTAT 
ATCAGGCAAT CCATGTCTAC AGTCTATCAA ATGTCACAGG AACGACAACA GAAGACAATT 
ACATCGACAA GTTTTGGAGC CGAAGAGTTT ATAATAATTA CGAATTTCTT GTTGAAACAG 
GCAACACCAA CTACTTTCAA AAAAGGTCAA AACTCATGGA TGGATGAAGT ACTGGAAACA 
TTCACACAAG GATGTCGTAC GCTTGAAAAA CCCGATTCAT ACGTTCCAGA GACATTTATC 
GAAAAATGGG ATTGGATTAT TAATCAGACT GCAAACTTTT GCATTGTGAA TAAAATGAAG 
ACACCATTAG GAAAACCAAT GCAGACTTTT GCAGCTTTTG AGAATGAAAT TAAACGACTT 
GCGAAAGAAG TGATTGTTCG AAAGAATAGT GATAAGAAGT TGAATAAATC ATCTACAGAA 
GATCCAAATC AATCTCCACC ACTCAAATAT TCCGTTCAAT GGCTTCGTGT TCATTTACTT 
CTAAAATTGA TTGTAGTTTT GGAAAAACTT ATGAACTCAG CCATTCATGG TGGTTCTTCC 
GTTTTCAATT TGACTGAAAT TCCAGTGTCC TCTCGCCAAT TTTTCACAGT GAACGCAGCA 
AGTTGTGAGG TTTGGCTGAA TCGAGTATAC TATCCTGCTC TTCTTGTCGC ATATTTCAAT 
GGATATTATG GACTTGTTAT TCGATTTGGT TCAAATGCTC TTAGCCATTT CGCGAGACAA 
AAAGACGGAG ATAATGATAA AAAGATCGTG AATGGCGTTT GTACAGCAAG TTTAATGTCA 
CTTTCAATGG CAGTTCTCGG TGAACCAATG GAAATTGTTG GATTACGGAG AAAAGTTCGA 
GAAGAATTCG GAACTGACAT GGGACAATCA TTGATGGAGG CACTCGGAGA AATGGCTAAT 
GCTAGATATG AGACAGCTCT GGTTGCCTTG GAAGCAGTTC TTGTGACAGA TGCAGCAACA 
AATGAAACAT TGAAAATGAT CATTCAACTA GCAATGGTTG ACATTTTGAA CCGCATACGC 
CTCCCACAGG CTACCGATTA TTATAAGGTT GTCTTATTTG GTGAAGAATC AAATGATTCC 
ACAATTACTG AAGACTTTCG ATCAGTTGAG TTACTCACGA AATTCGAGAA GCTCAACAAT 
ACAGTGAACG AAAAGCGTCA AGTTGTTGAT TGGAGTGCTC GAGAACGTTT CCAATTTGTT 
GAATCCGCAT TTTCACAGAC TATGAGAAGA ACCGAACTTC TCGACATTCA AAAAGATTTT 
TCCGCGATGG GAGCACTTGC ATTGAGTGCT GATTCATCGT GTAAATTGTA TTCGGACATT 
TCATCAACAT CTCTCATAAT TGCAAATCTT GTCAATAAAA TGACTGGTGT CTCACAATGG 
AAGAATAAAC TCACTGATAC GGAAATATTC GATAGAAATG AAGAAGGAAA TGATGGTGAT 
AAGTTGGCAA TATGTCGAAA GTTAATGCAT TGGGGACGAC ACACAAAGAG TAATCGCGGA 
CAGTCATGCG CTGCACACAG TGAAATAATT CGTTTATCCC GTAAAACTTC AAATTGTGAA 
CTCGCGTTCT TTCACATAAA TTCAGCTATT CGAGGTGAAA AACTAGCTGC TTGGCAGAGA 
TTAGAAGTGG AAAGACAGCG ACTGAAACTA GTGAAAACTC AAAATTTGGA TGTGCGAATT 
CGTGAAATGA ATGAAGTGTT CGGATCCTTG GCTGAAGTTT TTACGACAAG TTGTCAACTG 
AAATCAGACT TTCAAATGGT TGATGGAATG ATTAAAGAAA AAATGATCTC CGAAGGATAT 
AATGAAGATA TTGCAAAGCG TGAGGAGCAC ATGAGTCGAG CCAGTATTCA ACTTGCAGAC 
TTTTTCCAAT CACTTCCAGA ACTTGAAAAT GTACTTGCGC CCAACCTGTT CCCGACAATT 
ATTTGGAGTG AACTACAACG TCGATCAGAT AGTTTATCTG CTGGATATCA TGGAATAGTT 
GGATCTCTCT TTCATTTGGC ATCTGAAATG TGTCCATCTC TCGCTAAAGC TCATCTCAAA 
ATGGCCAGAT GGGCATATGA AATCGCTAAA ATCAAGAATT TCCCTGCTGA AAATTTAAGT 
TTCTACAAAT TTGGAAAAGA CGAAACTGAA AACGAGGAGC TATTGAAGAG CCTGGAAGCG 
ACGAGTCTTG TTAATTTGGA AAAGCTTGTT CGAGCTGCAA TTTCTGATGA TTTGAGAGCA 
AACAACATTC TGGCACCCAA TAGTCACTTT ATGCACATTT GGAAAATGGT TCGGGATCAT 
CGCACGAAAT TCTTGTCAAT TGCTGTGACT TCATATTTCC AATTTATTCA GAATATGTCC 
GGTGATTGTG ATAATCTACC GTACTCGAAA AAAGAGGAGA CCACTTTGGC GACATTGAGA 
ATTCTAGAAC TTCTTGTCAA ACATGGCGAT GTTCTCATTG ATGTAATAAA TGATGGACTA 
AATAAGACTA ATGTACATAT TTGGAAAGAA ATTCTTCCTC AATTGTTTGC TCGCTTGTCC 
CATCCATCTG AACATATTCG AAAAACTCTT GTTGATCTAA TTTCAAAAAT CTGTACAGCT 
GCACCACATG CAGTCGTTTT TCAAGTTGTT TCTGGTGCTG CATCATCTTC TACAGACGGT 
GAAGAACTTG AAGAGCAGCA GAATGATGAT CGTAACCGAG TACGTGCATG CTGTGAGAAA 
TTGGAGACAA ATATGTCACA ATCATATCCG AATCTTGTGA AAGATGTACG GCAGTTTGTA 
GCAGAACTTG AACGAATTAA TCTTTTGAAT GAAGAGAAAT GGTCTGTTGT CATGGGAACA 
ATGGAACATG AAATGGAAAA ACGATTATCA TTGATTAGAA CTGAAAATGC GAAAACTGAA 
TCGGCTCTTC ATTTAACAGC TTCAGTCAAA AATGATATTA TTGTGAAAAG AACCCAATTG 
TTGACTAGAC AGATATTTGA TGTACTCGAT GAGCTCTACC AACAAACAGT CATTGAGCCT 
CCAAAGAGCA AGAATGAAGA AGAGTTTGTA ACAGCTTTTG CCGAAGTACT CACAAACGCT 
TTTCAAGAGT CCAGAATATC ACGTACCACT TCTCCGGAAA AGTCATGGAT TCCATTTAAA 
AATCTCATTG CCAATTTTGT GCATCGAAAC AGCAAAAAAG GAATGCAAAC ATTTGAAACG 
GAAGACATTT CTCCGTACCT CGCATCTCTT TCCAATTCGT GTGTTCCAAT GCCAGGTCAA 
GAATCTGTCG AGTTTGATAG AGTTGTTTCG ATATCTCGTG TTGCTCGACA AGTCACAATT 
CTGCCCACGA AAACTCGCCC GAAAAAGCTG GGATTTGTCG GATCTGATGG AAAACAAGTT 
GCATTTTTAT TCAAAGGACG CGAAGATCTT CATCTTGATG AACGAGTCAT GCAATTCCTC 
CGATTATGTA ATGTGATGCT TCAACCAGGA AAAGGGAAAC ATCGACAATC GGTTGCCGCG 
TATCAGGCAC ATCATTATGC TGTTATTCCA CTTGGACCAC GTTCTGGACT TATCAAATGG 
GTTGAAGGTG CTACACCAAT GTTCCACATA TATCGTAAAT GGCAGATGAA AGAGCTTCGT 
CCATTGATAA GAGTAATTGG AAAGGAACGA GAAGAGTTTG CAGATTATTT GAAGTTTTAC 
AAGCAGGCAT TATTTGATCC ACTTTTGAAA GGACACTCGG CTCTCCGTAA CGAACTCGAT 
ATTGATACAT GTGTGTACAA TTTCAACATT GTCATGCAAA ATATAGATAA TGTGTTTGGA 
GCACTCGTCA ATCTGTCATT CACCCCGATT GAGACTATTA CATCCCGTAC TTCACAGCAA 
GAATTCAAAC CACCTCCCGG ACTTGAAAAT GTATGGGTTG TGAAACAAGA TCAACAGGAG 
AATTCTCAAG CGCGTGAAGT TGTCCGACGT GTTGAACGGC GTCTCAACGG ATGGCTCGAT 
GGATCGGCAG GACCAGATAG GAAATTGTCT CCTCGCGAAG AGGCCGACAT CCTAATCGCT 
GAAGCTACAT CCACACCAAA TTTATCTCAA ATGTACGAAG GATGGACTGC TTGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smg-1 ( NM_001374908.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001361976.1 Caenorhabditis elegans Serine/threonine-protein kinase smg-1 (smg-1), mRNA.

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CloneID	OCa850853
Clone ID Related Accession (Same CDS sequence)	NM_001374908.1
Accession Version	NM_001374908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein kinase smg-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279).

ATGATAACAT CGAGAAACAA TGATATTGGG AACCTGATAG AACAGTTTCG TCAACGTGAC 
ACGCCACAGA AGGAACGGAA AGCGATTCTC GCTAGAATCG AGGAGATTCT TCAAACTACG 
AAAAATGTAG AGAGTCTTTG TGTAAAATGG ACTTATCTTT TGGATAACTT GTGTTGGCCT 
AGTCTCACAA AACACGATCG GAATGACATG AAAACGCTGG CTGGAAAAGT GATACGACTT 
GTTGGAGTTT TACTCTTTGA TACTGAATCC TATCCAGAGT TTCTGATTTA TCTTGGAACA 
TTATATCAAA GTGTTACGAA GAAAAGTGAA GAAACACGTG CGGATATCGT GTTTTCAGTG 
TATTTCATCG TTGGAGTCAT CAGTCAAAAA ACGGAGAATC GTCTAATTGC TACCGACACT 
GAAAATGTCG AAAAATCATT AGACTGGATA ATCAAAGTGC TTCCGAATTC ATCAATATCA 
GTTTATAACC ACTGTTTGAA AGGATTTGTA CTTGTTGCAA ACACATTTCC AAATGTTTAT 
GCTGCAATGT TTGAGTCAAC CCTCCGAGCA ATTCTGACCA ACCTTCCCGA TTTCAACAGT 
CATGAAAAGA ATTTTGAGCT TCTCATTGAT ACAGTGATGC GATTTTCTGA TCAACTTAAT 
GAGAAGCCTC ATTTGGCAGA AGAAATGGTT CGAATTATTC GACCGGATAT CAAAAAGAAT 
GGATTGGGAA ATATGAGAGA ATTGAAGAAA CGAATGAAAC TGACAATGGC ACTCGTGAAA 
ATGGCGAAAA GTCAAAAAAT GCTTGAGGAG ACTAATCAGA TGATTTCTGA AATGTCTATC 
GAACTCGAGG AAAACGGAGG AAAATGGTCA TCAGCTTCAC TTATAACTAT CGTTTGTGAT 
GTTTTCAATG AGCTCTTGAT ATTGGGAAAA GATGATGTAA AATTGCAAAA AGGAGTTGAA 
GAATCATTGT GCAATGTTCT GAAAGACTTG AATCTTTCTA ATCAGAGTAC TATGGAGAAA 
CAAGCATTTT TCAATTCTCT TGCAAAGATT GTCAAACAAC TTCCAGCAGA GTCACAAGTC 
AAAACTAGAG TTCATCAGAT TGTTTTCAAC ACGGAAACTG GACTTTTCAC ACCCAAAAAC 
CGCGATAATC GAATGTTTGG ACATAATATG ATATACAAGG ATCTTATCAA TCTTGTCTCT 
GTGCTTCTTA CGCCTACATC ACTCAATCAT CTACAAGCGA CATATACGGA TTTGAGGAAA 
ATAATGATAG ATAGCATGTC ACGATTGAAG CAAAGTGAAG ATACTCCGTA TAGTGATAAT 
ATTCGATGGA ATGAATCAAT CCTTTTGCTT TTCTTTTCGT CACTTCAAAG TATATCATGT 
GCCAAGAGTT CTCTTATTGT GATGATGGGA ATCCGTCCGT CAATTTTTGA ATTCTTCTCA 
TCGGAACTTC CACTCACAGA ATATTGGCTT GCTTCAAATC ATCCAGAAGT CTATCACCTA 
TTTATCACAA TATTTGTTGG ACATTTGAAA GCTCACGACT TCTACATTGT TCAATCTGAT 
TATATTGTTC GTGGTGATAG CATTGGACAA AGTATAGGAC AAACGAAACG AGATTATGCA 
CGGAAACAAG TTGTTGCTCT ACAGAAAATT ATCAACAATT TTGGAGACAA ATTGTGGAAG 
AAGACTAGAC TGATGATATC TTCATGGCTT CACTCTCTCA TTGCAACTGC TTGTGAACAT 
CAAATCGGAA GTGATAGTTT CAGTCAACGA GAATGGGTTC GATTAAGGAA TACTGTCATT 
CATCAGTCGG TGCTCACATG GAATAATGAA TGTGTAAATC AAGCATTGAC CATCCTCTCC 
ACTGCTACAA AATGGTCAGA ACTGACGTCA GACATCCATC GAGATATTGC GGATAAAACG 
AAAAAAGCAA AGTGGAAGGA GGCAACGACA ATTTGGGAAT CTGGAGATTG TAATACTTAT 
ATCAGGCAAT CCATGTCTAC AGTCTATCAA ATGTCACAGG AACGACAACA GAAGACAATT 
ACATCGACAA GTTTTGGAGC CGAAGAGTTT ATAATAATTA CGAATTTCTT GTTGAAACAG 
GCAACACCAA CTACTTTCAA TTCCAGAAAA GGTCAAAACT CATGGATGGA TGAAGTACTG 
GAAACATTCA CACAAGGATG TCGTACGCTT GAAAAACCCG ATTCATACGT TCCAGAGACA 
TTTATCGAAA AATGGGATTG GATTATTAAT CAGACTGCAA ACTTTTGCAT TGTGAATAAA 
ATGAAGACAC CATTAGGAAA ACCAATGCAG ACTTTTGCAG CTTTTGAGAA TGAAATTAAA 
CGACTTGCGA AAGAAGTGAT TGTTCGAAAG AATAGTGATA AGAAGTTGAA TAAATCATCT 
ACAGAAGATC CAAATCAATC TCCACCACTC AAATATTCCG TTCAATGGCT TCGTGTTCAT 
TTACTTCTAA AATTGATTGT AGTTTTGGAA AAACTTATGA ACTCAGCCAT TCATGGTGGT 
TCTTCCGTTT TCAATTTGAC TGAAATTCCA GTGTCCTCTC GCCAATTTTT CACAGTGAAC 
GCAGCAAGTT GTGAGGTTTG GCTGAATCGA GTATACTATC CTGCTCTTCT TGTCGCATAT 
TTCAATGGAT ATTATGGACT TGTTATTCGA TTTGGTTCAA ATGCTCTTAG CCATTTCGCG 
AGACAAAAAG ACGGAGATAA TGATAAAAAG ATCGTGAATG GCGTTTGTAC AGCAAGTTTA 
ATGTCACTTT CAATGGCAGT TCTCGGTGAA CCAATGGAAA TTGTTGGATT ACGGAGAAAA 
GTTCGAGAAG AATTCGGAAC TGACATGGGA CAATCATTGA TGGAGGCACT CGGAGAAATG 
GCTAATGCTA GATATGAGAC AGCTCTGGTT GCCTTGGAAG CAGTTCTTGT GACAGATGCA 
GCAACAAATG AAACATTGAA AATGATCATT CAACTAGCAA TGGTTGACAT TTTGAACCGC 
ATACGCCTCC CACAGGCTAC CGATTATTAT AAGGTTGTCT TATTTGGTGA AGAATCAAAT 
GATTCCACAA TTACTGAAGA CTTTCGATCA GTTGAGTTAC TCACGAAATT CGAGAAGCTC 
AACAATACAG TGAACGAAAA GCGTCAAGTT GTTGATTGGA GTGCTCGAGA ACGTTTCCAA 
TTTGTTGAAT CCGCATTTTC ACAGACTATG AGAAGAACCG AACTTCTCGA CATTCAAAAA 
GATTTTTCCG CGATGGGAGC ACTTGCATTG AGTGCTGATT CATCGTGTAA ATTGTATTCG 
GACATTTCAT CAACATCTCT CATAATTGCA AATCTTGTCA ATAAAATGAC TGGTGTCTCA 
CAATGGAAGA ATAAACTCAC TGATACGGAA ATATTCGATA GAAATGAAGA AGGAAATGAT 
GGTGATAAGT TGGCAATATG TCGAAAGTTA ATGCATTGGG GACGACACAC AAAGAGTAAT 
CGCGGACAGT CATGCGCTGC ACACAGTGAA ATAATTCGTT TATCCCGTAA AACTTCAAAT 
TGTGAACTCG CGTTCTTTCA CATAAATTCA GCTATTCGAG GTGAAAAACT AGCTGCTTGG 
CAGAGATTAG AAGTGGAAAG ACAGCGACTG AAACTAGTGA AAACTCAAAA TTTGGATGTG 
CGAATTCGTG AAATGAATGA AGTGTTCGGA TCCTTGGCTG AAGTTTTTAC GACAAGTTGT 
CAACTGAAAT CAGACTTTCA AATGGTTGAT GGAATGATTA AAGAAAAAAT GATCTCCGAA 
GGATATAATG AAGATATTGC AAAGCGTGAG GAGCACATGA GTCGAGCCAG TATTCAACTT 
GCAGACTTTT TCCAATCACT TCCAGAACTT GAAAATGTAC TTGCGCCCAA CCTGTTCCCG 
ACAATTATTT GGAGTGAACT ACAACGTCGA TCAGATAGTT TATCTGCTGG ATATCATGGA 
ATAGTTGGAT CTCTCTTTCA TTTGGCATCT GAAATGTGTC CATCTCTCGC TAAAGCTCAT 
CTCAAAATGG CCAGATGGGC ATATGAAATC GCTAAAATCA AGAATTTCCC TGCTGAAAAT 
TTAAGTTTCT ACAAATTTGG AAAAGACGAA ACTGAAAACG AGGAGCTATT GAAGAGCCTG 
GAAGCGACGA GTCTTGTTAA TTTGGAAAAG CTTGTTCGAG CTGCAATTTC TGATGATTTG 
AGAGCAAACA ACATTCTGGC ACCCAATAGT CACTTTATGC ACATTTGGAA AATGGTTCGG 
GATCATCGCA CGAAATTCTT GTCAATTGCT GTGACTTCAT ATTTCCAATT TATTCAGAAT 
ATGTCCGGTG ATTGTGATAA TCTACCGTAC TCGAAAAAAG AGGAGACCAC TTTGGCGACA 
TTGAGAATTC TAGAACTTCT TGTCAAACAT GGCGATGTTC TCATTGATGT AATAAATGAT 
GGACTAAATA AGACTAATGT ACATATTTGG AAAGAAATTC TTCCTCAATT GTTTGCTCGC 
TTGTCCCATC CATCTGAACA TATTCGAAAA ACTCTTGTTG ATCTAATTTC AAAAATCTGT 
ACAGCTGCAC CACATGCAGT CGTTTTTCAA GTTGTTTCTG GTGCTGCATC ATCTTCTACA 
GACGGTGAAG AACTTGAAGA GCAGCAGAAT GATGATCGTA ACCGAGTACG TGCATGCTGT 
GAGAAATTGG AGACAAATAT GTCACAATCA TATCCGAATC TTGTGAAAGA TGTACGGCAG 
TTTGTAGCAG AACTTGAACG AATTAATCTT TTGAATGAAG AGAAATGGTC TGTTGTCATG 
GGAACAATGG AACATGAAAT GGAAAAACGA TTATCATTGA TTAGAACTGA AAATGCGAAA 
ACTGAATCGG CTCTTCATTT AACAGCTTCA GTCAAAAATG ATATTATTGT GAAAAGAACC 
CAATTGTTGA CTAGACAGAT ATTTGATGTA CTCGATGAGC TCTACCAACA AACAGTCATT 
GAGCCTCCAA AGAGCAAGAA TGAAGAAGAG TTTGTAACAG CTTTTGCCGA AGTACTCACA 
AACGCTTTTC AAGAGTCCAG AATATCACGT ACCACTTCTC CGGAAAAGTC ATGGATTCCA 
TTTAAAAATC TCATTGCCAA TTTTGTGCAT CGAAACAGCA AAAAAGGAAT GCAAACATTT 
GAAACGGAAG ACATTTCTCC GTACCTCGCA TCTCTTTCCA ATTCGTGTGT TCCAATGCCA 
GGTCAAGAAT CTGTCGAGTT TGATAGAGTT GTTTCGATAT CTCGTGTTGC TCGACAAGTC 
ACAATTCTGC CCACGAAAAC TCGCCCGAAA AAGCTGGGAT TTGTCGGATC TGATGGAAAA 
CAAGTTGCAT TTTTATTCAA AGGACGCGAA GATCTTCATC TTGATGAACG AGTCATGCAA 
TTCCTCCGAT TATGTAATGT GATGCTTCAA CCAGGAAAAG GGAAACATCG ACAATCGGTT 
GCCGCGTATC AGGCACATCA TTATGCTGTT ATTCCACTTG GACCACGTTC TGGACTTATC 
AAATGGGTTG AAGGTGCTAC ACCAATGTTC CACATATATC GTAAATGGCA GATGAAAGAG 
AAAGCACTCA AACAAGCGAC CAAAAAGAAC GGAGAAACAG TTCCTGAAAT CGAAAGACCC 
AGCAACATGT ATCACAATAT GATTCGTCTA GCATTCGCAG ATCATAAAAT CGATTCCTCC 
ATCACCTCGG ATCGTTCTAA ATGGCCAGCA GAAATTTTGG AAGAAGTATT TGAAAGTCTC 
ACAGCAAAAA CACCAACAGA TTTAATCAGT CGTGAGCTTT GGATGCGTGC GAATGATGCA 
ACAACTTGGT GGTCGGTTAC AAAACGATAT TCAAGATCAC TGGCAGTCAT GAGTATGGTT 
GGATCAGTTT TAGGACTCGG TGATCGACAT TTGGACAATT TATTGGTTGA TTTGAAGTGG 
GGTCATGTAG TACATATTGA CTACAACATT TGCTTTGACA AGGGAAAGAA TCTGCGTATC 
CCCGAAACAG TCCCATTCCG TTTGACACGT AACATGCGTC ACGCTCTCGG ACCATCTGAA 
ATGTATGGTA CCTTCCGTGA ATCTTGTGTT CACGTGCTCT CAACTCTTCG TTCTGGTCAT 
CAAGTTCTTA CAATGCTTCT CGATGCATTT GTATTCGATC CGCTTGTCGA TTGGACATCA 
CATGAACATA CAGCAACTTC TGGTGTCTCA TTGGCACTAC AATTGGCTGT TTATGGAAGT 
AATTGGAAGA CAAAAGCTAA AGAAAGATTG ACCGATGCGA TGGAGTTACT CAATTTGAGA 
ATGTCTGAGG TTCAAACCCT TTGGCTTGCA AATCGTGATG ACCTTCTCCA CTGGATGAAA 
CAAGTGACCG AATGTCTTTT GATTGAAAAT TCGATGCTTG GAGCAAATGC CATATATGCT 
CAGCAACGTG TGAAAGCTGG AACGGAACTT CGTGAGGCTG TGACACGTCA TCATGCACTT 
GCCAAAGAGC TTCGTCCATT GATAAGAGTA ATTGGAAAGG AACGAGAAGA GTTTGCAGAT 
TATTTGAAGT TTTACAAGCA GGCATTATTT GATCCACTTT TGAAAGGACA CTCGGCTCTC 
CGTAACGAAC TCGATATTGA TACATGTGTG TACAATTTCA ACATTGTCAT GCAAAATATA 
GATAATGTGT TTGGAGCACT CGTCAATCTG TCATTCACCC CGATTGAGAC TATTACATCC 
CGTACTTCAC AGCAAGAATT CAAACCACCT CCCGGACTTG AAAATGTATG GGTTGTGAAA 
CAAGATCAAC AGGAGAATTC TCAAGCGCGT GAAGTTGTCC GACGTGTTGA ACGGCGTCTC 
AACGGATGGC TCGATGGATC GGCAGGACCA GATAGGAAAT TGTCTCCTCG CGAAGAGGCC 
GACATCCTAA TCGCTGAAGC TACATCCACA CCAAATTTAT CTCAAATGTA CGAAGGATGG 
ACTGCTTGGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361976.1
CDS	29..7003
Translation	MITSRNNDIGNLIEQFRQRDTPQKERKAILARIEEILQTTKNVESLCVKWTYLLDNLCWPSLTKHDRNDMKTLAGKVIRLVGVLLFDTESYPEFLIYLGTLYQSVTKKSEETRADIVFSVYFIVGVISQKTENRLIATDTENVEKSLDWIIKVLPNSSISVYNHCLKGFVLVANTFPNVYAAMFESTLRAILTNLPDFNSHEKNFELLIDTVMRFSDQLNEKPHLAEEMVRIIRPDIKKNGLGNMRELKKRMKLTMALVKMAKSQKMLEETNQMISEMSIELEENGGKWSSASLITIVCDVFNELLILGKDDVKLQKGVEESLCNVLKDLNLSNQSTMEKQAFFNSLAKIVKQLPAESQVKTRVHQIVFNTETGLFTPKNRDNRMFGHNMIYKDLINLVSVLLTPTSLNHLQATYTDLRKIMIDSMSRLKQSEDTPYSDNIRWNESILLLFFSSLQSISCAKSSLIVMMGIRPSIFEFFSSELPLTEYWLASNHPEVYHLFITIFVGHLKAHDFYIVQSDYIVRGDSIGQSIGQTKRDYARKQVVALQKIINNFGDKLWKKTRLMISSWLHSLIATACEHQIGSDSFSQREWVRLRNTVIHQSVLTWNNECVNQALTILSTATKWSELTSDIHRDIADKTKKAKWKEATTIWESGDCNTYIRQSMSTVYQMSQERQQKTITSTSFGAEEFIIITNFLLKQATPTTFNSRKGQNSWMDEVLETFTQGCRTLEKPDSYVPETFIEKWDWIINQTANFCIVNKMKTPLGKPMQTFAAFENEIKRLAKEVIVRKNSDKKLNKSSTEDPNQSPPLKYSVQWLRVHLLLKLIVVLEKLMNSAIHGGSSVFNLTEIPVSSRQFFTVNAASCEVWLNRVYYPALLVAYFNGYYGLVIRFGSNALSHFARQKDGDNDKKIVNGVCTASLMSLSMAVLGEPMEIVGLRRKVREEFGTDMGQSLMEALGEMANARYETALVALEAVLVTDAATNETLKMIIQLAMVDILNRIRLPQATDYYKVVLFGEESNDSTITEDFRSVELLTKFEKLNNTVNEKRQVVDWSARERFQFVESAFSQTMRRTELLDIQKDFSAMGALALSADSSCKLYSDISSTSLIIANLVNKMTGVSQWKNKLTDTEIFDRNEEGNDGDKLAICRKLMHWGRHTKSNRGQSCAAHSEIIRLSRKTSNCELAFFHINSAIRGEKLAAWQRLEVERQRLKLVKTQNLDVRIREMNEVFGSLAEVFTTSCQLKSDFQMVDGMIKEKMISEGYNEDIAKREEHMSRASIQLADFFQSLPELENVLAPNLFPTIIWSELQRRSDSLSAGYHGIVGSLFHLASEMCPSLAKAHLKMARWAYEIAKIKNFPAENLSFYKFGKDETENEELLKSLEATSLVNLEKLVRAAISDDLRANNILAPNSHFMHIWKMVRDHRTKFLSIAVTSYFQFIQNMSGDCDNLPYSKKEETTLATLRILELLVKHGDVLIDVINDGLNKTNVHIWKEILPQLFARLSHPSEHIRKTLVDLISKICTAAPHAVVFQVVSGAASSSTDGEELEEQQNDDRNRVRACCEKLETNMSQSYPNLVKDVRQFVAELERINLLNEEKWSVVMGTMEHEMEKRLSLIRTENAKTESALHLTASVKNDIIVKRTQLLTRQIFDVLDELYQQTVIEPPKSKNEEEFVTAFAEVLTNAFQESRISRTTSPEKSWIPFKNLIANFVHRNSKKGMQTFETEDISPYLASLSNSCVPMPGQESVEFDRVVSISRVARQVTILPTKTRPKKLGFVGSDGKQVAFLFKGREDLHLDERVMQFLRLCNVMLQPGKGKHRQSVAAYQAHHYAVIPLGPRSGLIKWVEGATPMFHIYRKWQMKEKALKQATKKNGETVPEIERPSNMYHNMIRLAFADHKIDSSITSDRSKWPAEILEEVFESLTAKTPTDLISRELWMRANDATTWWSVTKRYSRSLAVMSMVGSVLGLGDRHLDNLLVDLKWGHVVHIDYNICFDKGKNLRIPETVPFRLTRNMRHALGPSEMYGTFRESCVHVLSTLRSGHQVLTMLLDAFVFDPLVDWTSHEHTATSGVSLALQLAVYGSNWKTKAKERLTDAMELLNLRMSEVQTLWLANRDDLLHWMKQVTECLLIENSMLGANAIYAQQRVKAGTELREAVTRHHALAKELRPLIRVIGKEREEFADYLKFYKQALFDPLLKGHSALRNELDIDTCVYNFNIVMQNIDNVFGALVNLSFTPIETITSRTSQQEFKPPPGLENVWVVKQDQQENSQAREVVRRVERRLNGWLDGSAGPDRKLSPREEADILIAEATSTPNLSQMYEGWTAWV

Target ORF information:

RefSeq Version	NM_001374908.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein kinase smg-1 (smg-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374908.1

ORF Insert Sequence:

ATGATAACAT CGAGAAACAA TGATATTGGG AACCTGATAG AACAGTTTCG TCAACGTGAC 
ACGCCACAGA AGGAACGGAA AGCGATTCTC GCTAGAATCG AGGAGATTCT TCAAACTACG 
AAAAATGTAG AGAGTCTTTG TGTAAAATGG ACTTATCTTT TGGATAACTT GTGTTGGCCT 
AGTCTCACAA AACACGATCG GAATGACATG AAAACGCTGG CTGGAAAAGT GATACGACTT 
GTTGGAGTTT TACTCTTTGA TACTGAATCC TATCCAGAGT TTCTGATTTA TCTTGGAACA 
TTATATCAAA GTGTTACGAA GAAAAGTGAA GAAACACGTG CGGATATCGT GTTTTCAGTG 
TATTTCATCG TTGGAGTCAT CAGTCAAAAA ACGGAGAATC GTCTAATTGC TACCGACACT 
GAAAATGTCG AAAAATCATT AGACTGGATA ATCAAAGTGC TTCCGAATTC ATCAATATCA 
GTTTATAACC ACTGTTTGAA AGGATTTGTA CTTGTTGCAA ACACATTTCC AAATGTTTAT 
GCTGCAATGT TTGAGTCAAC CCTCCGAGCA ATTCTGACCA ACCTTCCCGA TTTCAACAGT 
CATGAAAAGA ATTTTGAGCT TCTCATTGAT ACAGTGATGC GATTTTCTGA TCAACTTAAT 
GAGAAGCCTC ATTTGGCAGA AGAAATGGTT CGAATTATTC GACCGGATAT CAAAAAGAAT 
GGATTGGGAA ATATGAGAGA ATTGAAGAAA CGAATGAAAC TGACAATGGC ACTCGTGAAA 
ATGGCGAAAA GTCAAAAAAT GCTTGAGGAG ACTAATCAGA TGATTTCTGA AATGTCTATC 
GAACTCGAGG AAAACGGAGG AAAATGGTCA TCAGCTTCAC TTATAACTAT CGTTTGTGAT 
GTTTTCAATG AGCTCTTGAT ATTGGGAAAA GATGATGTAA AATTGCAAAA AGGAGTTGAA 
GAATCATTGT GCAATGTTCT GAAAGACTTG AATCTTTCTA ATCAGAGTAC TATGGAGAAA 
CAAGCATTTT TCAATTCTCT TGCAAAGATT GTCAAACAAC TTCCAGCAGA GTCACAAGTC 
AAAACTAGAG TTCATCAGAT TGTTTTCAAC ACGGAAACTG GACTTTTCAC ACCCAAAAAC 
CGCGATAATC GAATGTTTGG ACATAATATG ATATACAAGG ATCTTATCAA TCTTGTCTCT 
GTGCTTCTTA CGCCTACATC ACTCAATCAT CTACAAGCGA CATATACGGA TTTGAGGAAA 
ATAATGATAG ATAGCATGTC ACGATTGAAG CAAAGTGAAG ATACTCCGTA TAGTGATAAT 
ATTCGATGGA ATGAATCAAT CCTTTTGCTT TTCTTTTCGT CACTTCAAAG TATATCATGT 
GCCAAGAGTT CTCTTATTGT GATGATGGGA ATCCGTCCGT CAATTTTTGA ATTCTTCTCA 
TCGGAACTTC CACTCACAGA ATATTGGCTT GCTTCAAATC ATCCAGAAGT CTATCACCTA 
TTTATCACAA TATTTGTTGG ACATTTGAAA GCTCACGACT TCTACATTGT TCAATCTGAT 
TATATTGTTC GTGGTGATAG CATTGGACAA AGTATAGGAC AAACGAAACG AGATTATGCA 
CGGAAACAAG TTGTTGCTCT ACAGAAAATT ATCAACAATT TTGGAGACAA ATTGTGGAAG 
AAGACTAGAC TGATGATATC TTCATGGCTT CACTCTCTCA TTGCAACTGC TTGTGAACAT 
CAAATCGGAA GTGATAGTTT CAGTCAACGA GAATGGGTTC GATTAAGGAA TACTGTCATT 
CATCAGTCGG TGCTCACATG GAATAATGAA TGTGTAAATC AAGCATTGAC CATCCTCTCC 
ACTGCTACAA AATGGTCAGA ACTGACGTCA GACATCCATC GAGATATTGC GGATAAAACG 
AAAAAAGCAA AGTGGAAGGA GGCAACGACA ATTTGGGAAT CTGGAGATTG TAATACTTAT 
ATCAGGCAAT CCATGTCTAC AGTCTATCAA ATGTCACAGG AACGACAACA GAAGACAATT 
ACATCGACAA GTTTTGGAGC CGAAGAGTTT ATAATAATTA CGAATTTCTT GTTGAAACAG 
GCAACACCAA CTACTTTCAA TTCCAGAAAA GGTCAAAACT CATGGATGGA TGAAGTACTG 
GAAACATTCA CACAAGGATG TCGTACGCTT GAAAAACCCG ATTCATACGT TCCAGAGACA 
TTTATCGAAA AATGGGATTG GATTATTAAT CAGACTGCAA ACTTTTGCAT TGTGAATAAA 
ATGAAGACAC CATTAGGAAA ACCAATGCAG ACTTTTGCAG CTTTTGAGAA TGAAATTAAA 
CGACTTGCGA AAGAAGTGAT TGTTCGAAAG AATAGTGATA AGAAGTTGAA TAAATCATCT 
ACAGAAGATC CAAATCAATC TCCACCACTC AAATATTCCG TTCAATGGCT TCGTGTTCAT 
TTACTTCTAA AATTGATTGT AGTTTTGGAA AAACTTATGA ACTCAGCCAT TCATGGTGGT 
TCTTCCGTTT TCAATTTGAC TGAAATTCCA GTGTCCTCTC GCCAATTTTT CACAGTGAAC 
GCAGCAAGTT GTGAGGTTTG GCTGAATCGA GTATACTATC CTGCTCTTCT TGTCGCATAT 
TTCAATGGAT ATTATGGACT TGTTATTCGA TTTGGTTCAA ATGCTCTTAG CCATTTCGCG 
AGACAAAAAG ACGGAGATAA TGATAAAAAG ATCGTGAATG GCGTTTGTAC AGCAAGTTTA 
ATGTCACTTT CAATGGCAGT TCTCGGTGAA CCAATGGAAA TTGTTGGATT ACGGAGAAAA 
GTTCGAGAAG AATTCGGAAC TGACATGGGA CAATCATTGA TGGAGGCACT CGGAGAAATG 
GCTAATGCTA GATATGAGAC AGCTCTGGTT GCCTTGGAAG CAGTTCTTGT GACAGATGCA 
GCAACAAATG AAACATTGAA AATGATCATT CAACTAGCAA TGGTTGACAT TTTGAACCGC 
ATACGCCTCC CACAGGCTAC CGATTATTAT AAGGTTGTCT TATTTGGTGA AGAATCAAAT 
GATTCCACAA TTACTGAAGA CTTTCGATCA GTTGAGTTAC TCACGAAATT CGAGAAGCTC 
AACAATACAG TGAACGAAAA GCGTCAAGTT GTTGATTGGA GTGCTCGAGA ACGTTTCCAA 
TTTGTTGAAT CCGCATTTTC ACAGACTATG AGAAGAACCG AACTTCTCGA CATTCAAAAA 
GATTTTTCCG CGATGGGAGC ACTTGCATTG AGTGCTGATT CATCGTGTAA ATTGTATTCG 
GACATTTCAT CAACATCTCT CATAATTGCA AATCTTGTCA ATAAAATGAC TGGTGTCTCA 
CAATGGAAGA ATAAACTCAC TGATACGGAA ATATTCGATA GAAATGAAGA AGGAAATGAT 
GGTGATAAGT TGGCAATATG TCGAAAGTTA ATGCATTGGG GACGACACAC AAAGAGTAAT 
CGCGGACAGT CATGCGCTGC ACACAGTGAA ATAATTCGTT TATCCCGTAA AACTTCAAAT 
TGTGAACTCG CGTTCTTTCA CATAAATTCA GCTATTCGAG GTGAAAAACT AGCTGCTTGG 
CAGAGATTAG AAGTGGAAAG ACAGCGACTG AAACTAGTGA AAACTCAAAA TTTGGATGTG 
CGAATTCGTG AAATGAATGA AGTGTTCGGA TCCTTGGCTG AAGTTTTTAC GACAAGTTGT 
CAACTGAAAT CAGACTTTCA AATGGTTGAT GGAATGATTA AAGAAAAAAT GATCTCCGAA 
GGATATAATG AAGATATTGC AAAGCGTGAG GAGCACATGA GTCGAGCCAG TATTCAACTT 
GCAGACTTTT TCCAATCACT TCCAGAACTT GAAAATGTAC TTGCGCCCAA CCTGTTCCCG 
ACAATTATTT GGAGTGAACT ACAACGTCGA TCAGATAGTT TATCTGCTGG ATATCATGGA 
ATAGTTGGAT CTCTCTTTCA TTTGGCATCT GAAATGTGTC CATCTCTCGC TAAAGCTCAT 
CTCAAAATGG CCAGATGGGC ATATGAAATC GCTAAAATCA AGAATTTCCC TGCTGAAAAT 
TTAAGTTTCT ACAAATTTGG AAAAGACGAA ACTGAAAACG AGGAGCTATT GAAGAGCCTG 
GAAGCGACGA GTCTTGTTAA TTTGGAAAAG CTTGTTCGAG CTGCAATTTC TGATGATTTG 
AGAGCAAACA ACATTCTGGC ACCCAATAGT CACTTTATGC ACATTTGGAA AATGGTTCGG 
GATCATCGCA CGAAATTCTT GTCAATTGCT GTGACTTCAT ATTTCCAATT TATTCAGAAT 
ATGTCCGGTG ATTGTGATAA TCTACCGTAC TCGAAAAAAG AGGAGACCAC TTTGGCGACA 
TTGAGAATTC TAGAACTTCT TGTCAAACAT GGCGATGTTC TCATTGATGT AATAAATGAT 
GGACTAAATA AGACTAATGT ACATATTTGG AAAGAAATTC TTCCTCAATT GTTTGCTCGC 
TTGTCCCATC CATCTGAACA TATTCGAAAA ACTCTTGTTG ATCTAATTTC AAAAATCTGT 
ACAGCTGCAC CACATGCAGT CGTTTTTCAA GTTGTTTCTG GTGCTGCATC ATCTTCTACA 
GACGGTGAAG AACTTGAAGA GCAGCAGAAT GATGATCGTA ACCGAGTACG TGCATGCTGT 
GAGAAATTGG AGACAAATAT GTCACAATCA TATCCGAATC TTGTGAAAGA TGTACGGCAG 
TTTGTAGCAG AACTTGAACG AATTAATCTT TTGAATGAAG AGAAATGGTC TGTTGTCATG 
GGAACAATGG AACATGAAAT GGAAAAACGA TTATCATTGA TTAGAACTGA AAATGCGAAA 
ACTGAATCGG CTCTTCATTT AACAGCTTCA GTCAAAAATG ATATTATTGT GAAAAGAACC 
CAATTGTTGA CTAGACAGAT ATTTGATGTA CTCGATGAGC TCTACCAACA AACAGTCATT 
GAGCCTCCAA AGAGCAAGAA TGAAGAAGAG TTTGTAACAG CTTTTGCCGA AGTACTCACA 
AACGCTTTTC AAGAGTCCAG AATATCACGT ACCACTTCTC CGGAAAAGTC ATGGATTCCA 
TTTAAAAATC TCATTGCCAA TTTTGTGCAT CGAAACAGCA AAAAAGGAAT GCAAACATTT 
GAAACGGAAG ACATTTCTCC GTACCTCGCA TCTCTTTCCA ATTCGTGTGT TCCAATGCCA 
GGTCAAGAAT CTGTCGAGTT TGATAGAGTT GTTTCGATAT CTCGTGTTGC TCGACAAGTC 
ACAATTCTGC CCACGAAAAC TCGCCCGAAA AAGCTGGGAT TTGTCGGATC TGATGGAAAA 
CAAGTTGCAT TTTTATTCAA AGGACGCGAA GATCTTCATC TTGATGAACG AGTCATGCAA 
TTCCTCCGAT TATGTAATGT GATGCTTCAA CCAGGAAAAG GGAAACATCG ACAATCGGTT 
GCCGCGTATC AGGCACATCA TTATGCTGTT ATTCCACTTG GACCACGTTC TGGACTTATC 
AAATGGGTTG AAGGTGCTAC ACCAATGTTC CACATATATC GTAAATGGCA GATGAAAGAG 
AAAGCACTCA AACAAGCGAC CAAAAAGAAC GGAGAAACAG TTCCTGAAAT CGAAAGACCC 
AGCAACATGT ATCACAATAT GATTCGTCTA GCATTCGCAG ATCATAAAAT CGATTCCTCC 
ATCACCTCGG ATCGTTCTAA ATGGCCAGCA GAAATTTTGG AAGAAGTATT TGAAAGTCTC 
ACAGCAAAAA CACCAACAGA TTTAATCAGT CGTGAGCTTT GGATGCGTGC GAATGATGCA 
ACAACTTGGT GGTCGGTTAC AAAACGATAT TCAAGATCAC TGGCAGTCAT GAGTATGGTT 
GGATCAGTTT TAGGACTCGG TGATCGACAT TTGGACAATT TATTGGTTGA TTTGAAGTGG 
GGTCATGTAG TACATATTGA CTACAACATT TGCTTTGACA AGGGAAAGAA TCTGCGTATC 
CCCGAAACAG TCCCATTCCG TTTGACACGT AACATGCGTC ACGCTCTCGG ACCATCTGAA 
ATGTATGGTA CCTTCCGTGA ATCTTGTGTT CACGTGCTCT CAACTCTTCG TTCTGGTCAT 
CAAGTTCTTA CAATGCTTCT CGATGCATTT GTATTCGATC CGCTTGTCGA TTGGACATCA 
CATGAACATA CAGCAACTTC TGGTGTCTCA TTGGCACTAC AATTGGCTGT TTATGGAAGT 
AATTGGAAGA CAAAAGCTAA AGAAAGATTG ACCGATGCGA TGGAGTTACT CAATTTGAGA 
ATGTCTGAGG TTCAAACCCT TTGGCTTGCA AATCGTGATG ACCTTCTCCA CTGGATGAAA 
CAAGTGACCG AATGTCTTTT GATTGAAAAT TCGATGCTTG GAGCAAATGC CATATATGCT 
CAGCAACGTG TGAAAGCTGG AACGGAACTT CGTGAGGCTG TGACACGTCA TCATGCACTT 
GCCAAAGAGC TTCGTCCATT GATAAGAGTA ATTGGAAAGG AACGAGAAGA GTTTGCAGAT 
TATTTGAAGT TTTACAAGCA GGCATTATTT GATCCACTTT TGAAAGGACA CTCGGCTCTC 
CGTAACGAAC TCGATATTGA TACATGTGTG TACAATTTCA ACATTGTCAT GCAAAATATA 
GATAATGTGT TTGGAGCACT CGTCAATCTG TCATTCACCC CGATTGAGAC TATTACATCC 
CGTACTTCAC AGCAAGAATT CAAACCACCT CCCGGACTTG AAAATGTATG GGTTGTGAAA 
CAAGATCAAC AGGAGAATTC TCAAGCGCGT GAAGTTGTCC GACGTGTTGA ACGGCGTCTC 
AACGGATGGC TCGATGGATC GGCAGGACCA GATAGGAAAT TGTCTCCTCG CGAAGAGGCC 
GACATCCTAA TCGCTGAAGC TACATCCACA CCAAATTTAT CTCAAATGTA CGAAGGATGG 
ACTGCTTGGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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smg-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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