NEDD4L gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006073366.1	XP_006073428.1	E3 ubiquitin-protein ligase NEDD4-like isoform X4
XM_006073367.1	XP_006073429.1	E3 ubiquitin-protein ligase NEDD4-like isoform X5
XM_006073368.2	XP_006073430.1	E3 ubiquitin-protein ligase NEDD4-like isoform X7
XM_006073371.2	XP_006073433.1	E3 ubiquitin-protein ligase NEDD4-like isoform X8
XM_025273169.1	XP_025128954.1	E3 ubiquitin-protein ligase NEDD4-like isoform X11
XM_006073372.1	XP_006073434.1	E3 ubiquitin-protein ligase NEDD4-like isoform X10
XM_006073373.1	XP_006073435.1	E3 ubiquitin-protein ligase NEDD4-like isoform X11
XM_006073363.1	XP_006073425.1	E3 ubiquitin-protein ligase NEDD4-like isoform X1
XM_006073368.1	XP_006073430.1	E3 ubiquitin-protein ligase NEDD4-like isoform X6
XM_006073363.2	XP_006073425.1	E3 ubiquitin-protein ligase NEDD4-like isoform X1
XM_006073364.2	XP_006073426.1	E3 ubiquitin-protein ligase NEDD4-like isoform X2
XM_025273166.1	XP_025128951.1	E3 ubiquitin-protein ligase NEDD4-like isoform X5
XM_025273167.1	XP_025128952.1	E3 ubiquitin-protein ligase NEDD4-like isoform X9
XM_006073371.1	XP_006073433.1	E3 ubiquitin-protein ligase NEDD4-like isoform X9
XM_025273168.1	XP_025128953.1	E3 ubiquitin-protein ligase NEDD4-like isoform X10
XM_006073372.2	XP_006073434.1	E3 ubiquitin-protein ligase NEDD4-like isoform X12
XM_006073365.2	XP_006073427.1	E3 ubiquitin-protein ligase NEDD4-like isoform X3
XM_006073366.2	XP_006073428.1	E3 ubiquitin-protein ligase NEDD4-like isoform X6
XM_006073364.1	XP_006073426.1	E3 ubiquitin-protein ligase NEDD4-like isoform X2
XM_006073365.1	XP_006073427.1	E3 ubiquitin-protein ligase NEDD4-like isoform X3
XM_006073369.1	XP_006073431.1	E3 ubiquitin-protein ligase NEDD4-like isoform X7
XM_006073370.1	XP_006073432.1	E3 ubiquitin-protein ligase NEDD4-like isoform X8
XM_025273165.1	XP_025128950.1	E3 ubiquitin-protein ligase NEDD4-like isoform X4
XM_006073373.2	XP_006073435.1	E3 ubiquitin-protein ligase NEDD4-like isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu27441	XM_006073367.1 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu27443	XM_006073369.1 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu27444	XM_006073370.1 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu27446	XM_006073373.1 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu27446	XM_006073372.1 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu58355	XM_006073368.2 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu58356	XM_006073371.2 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu58357	XM_025273167.1 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu58358	XM_025273168.1 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu58359	XM_025273169.1 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu58360	XM_006073373.2 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu58360	XM_006073372.2 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu58349	XM_006073363.2 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu58350	XM_006073364.2 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu58351	XM_006073365.2 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu58352	XM_025273165.1 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu58353	XM_025273166.1 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu58354	XM_006073366.2 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu27437	XM_006073363.1 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBu27438	XM_006073364.1 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBu27439	XM_006073365.1 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBu27440	XM_006073366.1 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBu27442	XM_006073368.1 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBu27445	XM_006073371.1 Latest version!	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

NEDD4L ( XM_006073367.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073429.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu27441
Clone ID Related Accession (Same CDS sequence)	XM_006073367.1
Accession Version	XM_006073367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785536.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGT GGCCTGATGC TTTTCACCAG GGAGAGTCCC GTATTCTCAG AGTGAAGGTT 
GTTTCTGGAA TTGATCTCGC CAAGAAGGAC ATCTTCGGAG CCAGTGATCC GTATGTGAAA 
CTTTCATTGT ACGTAGCGGA TGAGAATAGG GAACTTGCTT TGGTACAAAC AAAAACAATT 
AAAAAGACGC TGAACCCAAA GTGGAATGAA GAATTTTATT TTAGAGTAAA TCCATCTAAT 
CACAGACTCC TGTTTGAAGT ATTTGATGAA AACAGACTGA CACGGGACGA CTTCCTGGGA 
CAGGTGGACA TACCCCTTAG TCATCTCCCG ACAGAAGACC CAACCATGGA GCGACCCTAC 
ACATTTAAGG ACTTTCTCCT CCGGCCAAGA AGTCATAAAT CTCGGGTTAA GGGATTTTTG 
CGATTGAAAA TGGCCTACAT GCCCAAAAAC GGGGGCCAAG ATGAAGAGAA CAGTGAGCAG 
CGGGATGAGA TGGAGCCCGG GTGGGAAGTC GTTGACTCCA ATGACTCGGC TTCTCAGCAC 
CAGGAGGAGC TGCCCCCGCC TCCGCTGCCC CCCGGGTGGG AAGAAAAGGT GGACAACCTG 
GGCCGCACGT ACTACGTGAA CCACAACAAC CGGACCACCC AGTGGCACCG GCCCAGCCTC 
ATGGACGTGT CGTCCGAGTC CGACAGCAAC ATCCGGCAGA TCAACCAGGA GGCCGCGCAT 
CGCCGCTTCC GCTCGCGCAG ACATATCAGC GAGGACCTGG AGCCGGAGCC CAGCGAGGGA 
GGGGAGGGCC CCGAGCCTTG GGAGACCATT TCAGAGGAAG TGAACATGAG CGGAGACTCG 
CTCAGCCTGG CGCTGCCCCC GCCACCAGCC TCGCCCGTGT CCCGGACGAG CCCCCAGGAG 
CTGTCCGAGG AGCTGAGCAG AAGGCTGCAG GTCACTCCCG ACTCCAACGG CGAGCAGTTT 
GGGTCTTTGA TTCAGCGAGA GCCGTCCTCC CGGCTGAGGT CCTGCAGTGT CACTGATGCA 
GTTGCGGAGC AGGCTCATCT CCCGCCGCCC AGTGCCCCAG CTGGGAGAGC ACGTTCATCA 
ACTGTCACCG GTGGTGAGGC GCCAACGCCA TCAGTGGCCT ATGTACACAC CACGCCGGGC 
CTGCCCTCGG GCTGGGAAGA GAGGAAAGAT GCTAAGGGAC GCACATACTA TGTCAATCAT 
AACAATCGAA CCACAACTTG GACTCGGCCT ATCGTGCAGC TCGCAGAAGA CGGCGCGTCC 
GGATCCGCCA CAAACAGTAA CAACCACCTA ATCGAGCCTC AGATCCGCCG GCCTCGTAGC 
CTCAGCTCGC CAACAGTAAC TTTATCCGCC CCGCTGGAGG GCGCCAAGGA CTCACCCGTA 
CGTCGGGCTG TGAAAGATAC CCTTTCCAAT CCACAGTCCC CACAGCCATC ACCTTACAAC 
TCCCCCAAAC CACAACACAA AGTCACACAG AGCTTCCTGC CGCCCGGCTG GGAGATGAGG 
ATAGCGCCCA ACGGCCGGCC CTTCTTCATT GACCACAACA CAAAGACCAC GACGTGGGAA 
GATCCACGCC TGAAGTTTCC AGTACACATG CGGTCAAAGG CATCCTTAAA CCCCAATGAC 
CTTGGCCCTC TTCCTCCTGG CTGGGAAGAA AGGATCCACT TGGATGGCCG GACGTTTTAC 
ATTGACCACA GCAGCCAGGT GTTATGTGGC GAGGACGATG CCAGAGACTC AGTGCCCTCG 
TACGATAGCA AAATTACTCA GTGGGAAGAC CCAAGACTGC AGAACCCAGC TATTACTGGT 
CCGGCTGTTC CTTACTCCAG AGAATTTAAG CAGAAATATG ACTACTTTAG AAAGAAATTA 
AAGAAACCTG CGGATATTCC AAATAGGTTT GAAATGAAAC TTCACAGAAA TAACATATTT 
GAAGAATCCT ATCGGAGAAT CATGTCTGTG AAAAGACCAG ATGTCCTGAA AGCTAGGCTG 
TGGATTGAGT TTGAATCGGA GAAAGGTCTG GACTACGGGG GCGTGGCCAG AGAGTGGTTC 
TTCCTACTGT CCAAGGAGAT GTTCAACCCT TACTACGGCC TCTTCGAGTA CTCGGCAACG 
GACAACTACA CACTTCAGAT CAACCCCAAT TCAGGCCTCT GTAATGAGGA CCATCTGTCC 
TATTTCACCT TCATTGGAAG AGTTGCCGGG CTGGCAGTAT TTCATGGGAA ACTTCTAGAT 
GGTTTCTTCA TTCGACCGTT TTATAAAATG ATGCTGGGAA AGCAGATAAC TCTGAATGAC 
ATGGAATCTG TGGATAGCGA ATATTACAAC TCTTTGAAAT GGATCTTAGA AAATGACCCA 
ACTGAACTGG ACCTTATGTT CTGCATTGAT GAAGAGAACT TTGGGCAGAC CTATCAAGTG 
GATCTGAAGC CCAACGGGTC AGAAATAATG GTAACAAATG AAAACAAGAG AGAGTACATC 
GACCTCGTTA TCCAGTGGAG GTTCGTGAAC AGAGTCCAGA AGCAGATGAA TGCCTTCCTG 
GAGGGATTCA CGGAACTGCT CCCTATTGAT TTGATTAAAA TCTTTGATGA AAATGAACTG 
GAGTTGCTGA TGTGCGGCCT TGGCGATGTG GATGTGAACG ACTGGCGACA ACATTCTATT 
TACAAGAATG GCTACTGCCC GAACCACCCC GTGATCCAGT GGTTCTGGAA GGCTGTGCTG 
CTGATGGACG CCGAGAGGCG CATCCGGTTA CTGCAGTTTG TCACAGGGAC GTCCCGAGTA 
CCTATGAATG GATTTGCCGA GCTTTATGGT TCCAATGGTC CTCAGCTGTT TACAATAGAG 
CAATGGGGAA GTCCTGAAAA ACTGCCCAGA GCTCACACGT GCTTTAATCG CCTTGACTTA 
CCTCCGTATG AAACCTTCGA AGATTTACGA GAGAAGCTCC TCATGGCTGT GGAAAACGCC 
CAAGGCTTCG AGGGGGTGGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073429.1
CDS	1..2964
Translation	MEPWPDAFHQGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSAPAGRARSSTVTGGEAPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHSSQVLCGEDDARDSVPSYDSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073367.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073367.1

ORF Insert Sequence:

ATGGAGCCGT GGCCTGATGC TTTTCACCAG GGAGAGTCCC GTATTCTCAG AGTGAAGGTT 
GTTTCTGGAA TTGATCTCGC CAAGAAGGAC ATCTTCGGAG CCAGTGATCC GTATGTGAAA 
CTTTCATTGT ACGTAGCGGA TGAGAATAGG GAACTTGCTT TGGTACAAAC AAAAACAATT 
AAAAAGACGC TGAACCCAAA GTGGAATGAA GAATTTTATT TTAGAGTAAA TCCATCTAAT 
CACAGACTCC TGTTTGAAGT ATTTGATGAA AACAGACTGA CACGGGACGA CTTCCTGGGA 
CAGGTGGACA TACCCCTTAG TCATCTCCCG ACAGAAGACC CAACCATGGA GCGACCCTAC 
ACATTTAAGG ACTTTCTCCT CCGGCCAAGA AGTCATAAAT CTCGGGTTAA GGGATTTTTG 
CGATTGAAAA TGGCCTACAT GCCCAAAAAC GGGGGCCAAG ATGAAGAGAA CAGTGAGCAG 
CGGGATGAGA TGGAGCCCGG GTGGGAAGTC GTTGACTCCA ATGACTCGGC TTCTCAGCAC 
CAGGAGGAGC TGCCCCCGCC TCCGCTGCCC CCCGGGTGGG AAGAAAAGGT GGACAACCTG 
GGCCGCACGT ACTACGTGAA CCACAACAAC CGGACCACCC AGTGGCACCG GCCCAGCCTC 
ATGGACGTGT CGTCCGAGTC CGACAGCAAC ATCCGGCAGA TCAACCAGGA GGCCGCGCAT 
CGCCGCTTCC GCTCGCGCAG ACATATCAGC GAGGACCTGG AGCCGGAGCC CAGCGAGGGA 
GGGGAGGGCC CCGAGCCTTG GGAGACCATT TCAGAGGAAG TGAACATGAG CGGAGACTCG 
CTCAGCCTGG CGCTGCCCCC GCCACCAGCC TCGCCCGTGT CCCGGACGAG CCCCCAGGAG 
CTGTCCGAGG AGCTGAGCAG AAGGCTGCAG GTCACTCCCG ACTCCAACGG CGAGCAGTTT 
GGGTCTTTGA TTCAGCGAGA GCCGTCCTCC CGGCTGAGGT CCTGCAGTGT CACTGATGCA 
GTTGCGGAGC AGGCTCATCT CCCGCCGCCC AGTGCCCCAG CTGGGAGAGC ACGTTCATCA 
ACTGTCACCG GTGGTGAGGC GCCAACGCCA TCAGTGGCCT ATGTACACAC CACGCCGGGC 
CTGCCCTCGG GCTGGGAAGA GAGGAAAGAT GCTAAGGGAC GCACATACTA TGTCAATCAT 
AACAATCGAA CCACAACTTG GACTCGGCCT ATCGTGCAGC TCGCAGAAGA CGGCGCGTCC 
GGATCCGCCA CAAACAGTAA CAACCACCTA ATCGAGCCTC AGATCCGCCG GCCTCGTAGC 
CTCAGCTCGC CAACAGTAAC TTTATCCGCC CCGCTGGAGG GCGCCAAGGA CTCACCCGTA 
CGTCGGGCTG TGAAAGATAC CCTTTCCAAT CCACAGTCCC CACAGCCATC ACCTTACAAC 
TCCCCCAAAC CACAACACAA AGTCACACAG AGCTTCCTGC CGCCCGGCTG GGAGATGAGG 
ATAGCGCCCA ACGGCCGGCC CTTCTTCATT GACCACAACA CAAAGACCAC GACGTGGGAA 
GATCCACGCC TGAAGTTTCC AGTACACATG CGGTCAAAGG CATCCTTAAA CCCCAATGAC 
CTTGGCCCTC TTCCTCCTGG CTGGGAAGAA AGGATCCACT TGGATGGCCG GACGTTTTAC 
ATTGACCACA GCAGCCAGGT GTTATGTGGC GAGGACGATG CCAGAGACTC AGTGCCCTCG 
TACGATAGCA AAATTACTCA GTGGGAAGAC CCAAGACTGC AGAACCCAGC TATTACTGGT 
CCGGCTGTTC CTTACTCCAG AGAATTTAAG CAGAAATATG ACTACTTTAG AAAGAAATTA 
AAGAAACCTG CGGATATTCC AAATAGGTTT GAAATGAAAC TTCACAGAAA TAACATATTT 
GAAGAATCCT ATCGGAGAAT CATGTCTGTG AAAAGACCAG ATGTCCTGAA AGCTAGGCTG 
TGGATTGAGT TTGAATCGGA GAAAGGTCTG GACTACGGGG GCGTGGCCAG AGAGTGGTTC 
TTCCTACTGT CCAAGGAGAT GTTCAACCCT TACTACGGCC TCTTCGAGTA CTCGGCAACG 
GACAACTACA CACTTCAGAT CAACCCCAAT TCAGGCCTCT GTAATGAGGA CCATCTGTCC 
TATTTCACCT TCATTGGAAG AGTTGCCGGG CTGGCAGTAT TTCATGGGAA ACTTCTAGAT 
GGTTTCTTCA TTCGACCGTT TTATAAAATG ATGCTGGGAA AGCAGATAAC TCTGAATGAC 
ATGGAATCTG TGGATAGCGA ATATTACAAC TCTTTGAAAT GGATCTTAGA AAATGACCCA 
ACTGAACTGG ACCTTATGTT CTGCATTGAT GAAGAGAACT TTGGGCAGAC CTATCAAGTG 
GATCTGAAGC CCAACGGGTC AGAAATAATG GTAACAAATG AAAACAAGAG AGAGTACATC 
GACCTCGTTA TCCAGTGGAG GTTCGTGAAC AGAGTCCAGA AGCAGATGAA TGCCTTCCTG 
GAGGGATTCA CGGAACTGCT CCCTATTGAT TTGATTAAAA TCTTTGATGA AAATGAACTG 
GAGTTGCTGA TGTGCGGCCT TGGCGATGTG GATGTGAACG ACTGGCGACA ACATTCTATT 
TACAAGAATG GCTACTGCCC GAACCACCCC GTGATCCAGT GGTTCTGGAA GGCTGTGCTG 
CTGATGGACG CCGAGAGGCG CATCCGGTTA CTGCAGTTTG TCACAGGGAC GTCCCGAGTA 
CCTATGAATG GATTTGCCGA GCTTTATGGT TCCAATGGTC CTCAGCTGTT TACAATAGAG 
CAATGGGGAA GTCCTGAAAA ACTGCCCAGA GCTCACACGT GCTTTAATCG CCTTGACTTA 
CCTCCGTATG AAACCTTCGA AGATTTACGA GAGAAGCTCC TCATGGCTGT GGAAAACGCC 
CAAGGCTTCG AGGGGGTGGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073369.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073431.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBu27443
Clone ID Related Accession (Same CDS sequence)	XM_006073369.1
Accession Version	XM_006073369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785536.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGT GGCCTGATGC TTTTCACCAG GGAGAGTCCC GTATTCTCAG AGTGAAGGTT 
GTTTCTGGAA TTGATCTCGC CAAGAAGGAC ATCTTCGGAG CCAGTGATCC GTATGTGAAA 
CTTTCATTGT ACGTAGCGGA TGAGAATAGG GAACTTGCTT TGGTACAAAC AAAAACAATT 
AAAAAGACGC TGAACCCAAA GTGGAATGAA GAATTTTATT TTAGAGTAAA TCCATCTAAT 
CACAGACTCC TGTTTGAAGT ATTTGATGAA AACAGACTGA CACGGGACGA CTTCCTGGGA 
CAGGTGGACA TACCCCTTAG TCATCTCCCG ACAGAAGACC CAACCATGGA GCGACCCTAC 
ACATTTAAGG ACTTTCTCCT CCGGCCAAGA AGTCATAAAT CTCGGGTTAA GGGATTTTTG 
CGATTGAAAA TGGCCTACAT GCCCAAAAAC GGGGGCCAAG ATGAAGAGAA CAGTGAGCAG 
CGGGATGAGA TGGAGCCCGG GTGGGAAGTC GTTGACTCCA ATGACTCGGC TTCTCAGCAC 
CAGGAGGAGC TGCCCCCGCC TCCGCTGCCC CCCGGGTGGG AAGAAAAGGT GGACAACCTG 
GGCCGCACGT ACTACGTGAA CCACAACAAC CGGACCACCC AGTGGCACCG GCCCAGCCTC 
ATGGACGTGT CGTCCGAGTC CGACAGCAAC ATCCGGCAGA TCAACCAGGA GGCCGCGCAT 
CGCCGCTTCC GCTCGCGCAG ACATATCAGC GAGGACCTGG AGCCGGAGCC CAGCGAGGGA 
GGGGAGGGCC CCGAGCCTTG GGAGACCATT TCAGAGGAAG TGAACATGAG CGGAGACTCG 
CTCAGCCTGG CGCTGCCCCC GCCACCAGCC TCGCCCGTGT CCCGGACGAG CCCCCAGGAG 
CTGTCCGAGG AGCTGAGCAG AAGGCTGCAG GTCACTCCCG ACTCCAACGG CGAGCAGTTT 
GGGTCTTTGA TTCAGCGAGA GCCGTCCTCC CGGCTGAGGT CCTGCAGTGT CACTGATGCA 
GTTGCGGAGC AGGCTCATCT CCCGCCGCCC AGTGCCCCAG CTGGGAGAGC ACGTTCATCA 
ACTGTCACCG GTGGTGAGGC GCCAACGCCA TCAGTGGCCT ATGTACACAC CACGCCGGGC 
CTGCCCTCGG GCTGGGAAGA GAGGAAAGAT GCTAAGGGAC GCACATACTA TGTCAATCAT 
AACAATCGAA CCACAACTTG GACTCGGCCT ATCGTGCAGC TCGCAGAAGA CGGCGCGTCC 
GGATCCGCCA CAAACAGTAA CAACCACCTA ATCGAGCCTC AGATCCGCCG GCCTCGTAGC 
CTCAGCTCGC CAACAGTAAC TTTATCCGCC CCGCTGGAGG GCGCCAAGGA CTCACCCGTA 
CGTCGGGCTG TGAAAGATAC CCTTTCCAAT CCACAGTCCC CACAGCCATC ACCTTACAAC 
TCCCCCAAAC CACAACACAA AGTCACACAG AGCTTCCTGC CGCCCGGCTG GGAGATGAGG 
ATAGCGCCCA ACGGCCGGCC CTTCTTCATT GACCACAACA CAAAGACCAC GACGTGGGAA 
GATCCACGCC TGAAGTTTCC AGTACACATG CGGTCAAAGG CATCCTTAAA CCCCAATGAC 
CTTGGCCCTC TTCCTCCTGG CTGGGAAGAA AGGATCCACT TGGATGGCCG GACGTTTTAC 
ATTGACCACA ATAGCAAAAT TACTCAGTGG GAAGACCCAA GACTGCAGAA CCCAGCTATT 
ACTGGTCCGG CTGTTCCTTA CTCCAGAGAA TTTAAGCAGA AATATGACTA CTTTAGAAAG 
AAATTAAAGA AACCTGCGGA TATTCCAAAT AGGTTTGAAA TGAAACTTCA CAGAAATAAC 
ATATTTGAAG AATCCTATCG GAGAATCATG TCTGTGAAAA GACCAGATGT CCTGAAAGCT 
AGGCTGTGGA TTGAGTTTGA ATCGGAGAAA GGTCTGGACT ACGGGGGCGT GGCCAGAGAG 
TGGTTCTTCC TACTGTCCAA GGAGATGTTC AACCCTTACT ACGGCCTCTT CGAGTACTCG 
GCAACGGACA ACTACACACT TCAGATCAAC CCCAATTCAG GCCTCTGTAA TGAGGACCAT 
CTGTCCTATT TCACCTTCAT TGGAAGAGTT GCCGGGCTGG CAGTATTTCA TGGGAAACTT 
CTAGATGGTT TCTTCATTCG ACCGTTTTAT AAAATGATGC TGGGAAAGCA GATAACTCTG 
AATGACATGG AATCTGTGGA TAGCGAATAT TACAACTCTT TGAAATGGAT CTTAGAAAAT 
GACCCAACTG AACTGGACCT TATGTTCTGC ATTGATGAAG AGAACTTTGG GCAGACCTAT 
CAAGTGGATC TGAAGCCCAA CGGGTCAGAA ATAATGGTAA CAAATGAAAA CAAGAGAGAG 
TACATCGACC TCGTTATCCA GTGGAGGTTC GTGAACAGAG TCCAGAAGCA GATGAATGCC 
TTCCTGGAGG GATTCACGGA ACTGCTCCCT ATTGATTTGA TTAAAATCTT TGATGAAAAT 
GAACTGGAGT TGCTGATGTG CGGCCTTGGC GATGTGGATG TGAACGACTG GCGACAACAT 
TCTATTTACA AGAATGGCTA CTGCCCGAAC CACCCCGTGA TCCAGTGGTT CTGGAAGGCT 
GTGCTGCTGA TGGACGCCGA GAGGCGCATC CGGTTACTGC AGTTTGTCAC AGGGACGTCC 
CGAGTACCTA TGAATGGATT TGCCGAGCTT TATGGTTCCA ATGGTCCTCA GCTGTTTACA 
ATAGAGCAAT GGGGAAGTCC TGAAAAACTG CCCAGAGCTC ACACGTGCTT TAATCGCCTT 
GACTTACCTC CGTATGAAAC CTTCGAAGAT TTACGAGAGA AGCTCCTCAT GGCTGTGGAA 
AACGCCCAAG GCTTCGAGGG GGTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073431.1
CDS	1..2910
Translation	MEPWPDAFHQGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSAPAGRARSSTVTGGEAPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073369.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073369.1

ORF Insert Sequence:

ATGGAGCCGT GGCCTGATGC TTTTCACCAG GGAGAGTCCC GTATTCTCAG AGTGAAGGTT 
GTTTCTGGAA TTGATCTCGC CAAGAAGGAC ATCTTCGGAG CCAGTGATCC GTATGTGAAA 
CTTTCATTGT ACGTAGCGGA TGAGAATAGG GAACTTGCTT TGGTACAAAC AAAAACAATT 
AAAAAGACGC TGAACCCAAA GTGGAATGAA GAATTTTATT TTAGAGTAAA TCCATCTAAT 
CACAGACTCC TGTTTGAAGT ATTTGATGAA AACAGACTGA CACGGGACGA CTTCCTGGGA 
CAGGTGGACA TACCCCTTAG TCATCTCCCG ACAGAAGACC CAACCATGGA GCGACCCTAC 
ACATTTAAGG ACTTTCTCCT CCGGCCAAGA AGTCATAAAT CTCGGGTTAA GGGATTTTTG 
CGATTGAAAA TGGCCTACAT GCCCAAAAAC GGGGGCCAAG ATGAAGAGAA CAGTGAGCAG 
CGGGATGAGA TGGAGCCCGG GTGGGAAGTC GTTGACTCCA ATGACTCGGC TTCTCAGCAC 
CAGGAGGAGC TGCCCCCGCC TCCGCTGCCC CCCGGGTGGG AAGAAAAGGT GGACAACCTG 
GGCCGCACGT ACTACGTGAA CCACAACAAC CGGACCACCC AGTGGCACCG GCCCAGCCTC 
ATGGACGTGT CGTCCGAGTC CGACAGCAAC ATCCGGCAGA TCAACCAGGA GGCCGCGCAT 
CGCCGCTTCC GCTCGCGCAG ACATATCAGC GAGGACCTGG AGCCGGAGCC CAGCGAGGGA 
GGGGAGGGCC CCGAGCCTTG GGAGACCATT TCAGAGGAAG TGAACATGAG CGGAGACTCG 
CTCAGCCTGG CGCTGCCCCC GCCACCAGCC TCGCCCGTGT CCCGGACGAG CCCCCAGGAG 
CTGTCCGAGG AGCTGAGCAG AAGGCTGCAG GTCACTCCCG ACTCCAACGG CGAGCAGTTT 
GGGTCTTTGA TTCAGCGAGA GCCGTCCTCC CGGCTGAGGT CCTGCAGTGT CACTGATGCA 
GTTGCGGAGC AGGCTCATCT CCCGCCGCCC AGTGCCCCAG CTGGGAGAGC ACGTTCATCA 
ACTGTCACCG GTGGTGAGGC GCCAACGCCA TCAGTGGCCT ATGTACACAC CACGCCGGGC 
CTGCCCTCGG GCTGGGAAGA GAGGAAAGAT GCTAAGGGAC GCACATACTA TGTCAATCAT 
AACAATCGAA CCACAACTTG GACTCGGCCT ATCGTGCAGC TCGCAGAAGA CGGCGCGTCC 
GGATCCGCCA CAAACAGTAA CAACCACCTA ATCGAGCCTC AGATCCGCCG GCCTCGTAGC 
CTCAGCTCGC CAACAGTAAC TTTATCCGCC CCGCTGGAGG GCGCCAAGGA CTCACCCGTA 
CGTCGGGCTG TGAAAGATAC CCTTTCCAAT CCACAGTCCC CACAGCCATC ACCTTACAAC 
TCCCCCAAAC CACAACACAA AGTCACACAG AGCTTCCTGC CGCCCGGCTG GGAGATGAGG 
ATAGCGCCCA ACGGCCGGCC CTTCTTCATT GACCACAACA CAAAGACCAC GACGTGGGAA 
GATCCACGCC TGAAGTTTCC AGTACACATG CGGTCAAAGG CATCCTTAAA CCCCAATGAC 
CTTGGCCCTC TTCCTCCTGG CTGGGAAGAA AGGATCCACT TGGATGGCCG GACGTTTTAC 
ATTGACCACA ATAGCAAAAT TACTCAGTGG GAAGACCCAA GACTGCAGAA CCCAGCTATT 
ACTGGTCCGG CTGTTCCTTA CTCCAGAGAA TTTAAGCAGA AATATGACTA CTTTAGAAAG 
AAATTAAAGA AACCTGCGGA TATTCCAAAT AGGTTTGAAA TGAAACTTCA CAGAAATAAC 
ATATTTGAAG AATCCTATCG GAGAATCATG TCTGTGAAAA GACCAGATGT CCTGAAAGCT 
AGGCTGTGGA TTGAGTTTGA ATCGGAGAAA GGTCTGGACT ACGGGGGCGT GGCCAGAGAG 
TGGTTCTTCC TACTGTCCAA GGAGATGTTC AACCCTTACT ACGGCCTCTT CGAGTACTCG 
GCAACGGACA ACTACACACT TCAGATCAAC CCCAATTCAG GCCTCTGTAA TGAGGACCAT 
CTGTCCTATT TCACCTTCAT TGGAAGAGTT GCCGGGCTGG CAGTATTTCA TGGGAAACTT 
CTAGATGGTT TCTTCATTCG ACCGTTTTAT AAAATGATGC TGGGAAAGCA GATAACTCTG 
AATGACATGG AATCTGTGGA TAGCGAATAT TACAACTCTT TGAAATGGAT CTTAGAAAAT 
GACCCAACTG AACTGGACCT TATGTTCTGC ATTGATGAAG AGAACTTTGG GCAGACCTAT 
CAAGTGGATC TGAAGCCCAA CGGGTCAGAA ATAATGGTAA CAAATGAAAA CAAGAGAGAG 
TACATCGACC TCGTTATCCA GTGGAGGTTC GTGAACAGAG TCCAGAAGCA GATGAATGCC 
TTCCTGGAGG GATTCACGGA ACTGCTCCCT ATTGATTTGA TTAAAATCTT TGATGAAAAT 
GAACTGGAGT TGCTGATGTG CGGCCTTGGC GATGTGGATG TGAACGACTG GCGACAACAT 
TCTATTTACA AGAATGGCTA CTGCCCGAAC CACCCCGTGA TCCAGTGGTT CTGGAAGGCT 
GTGCTGCTGA TGGACGCCGA GAGGCGCATC CGGTTACTGC AGTTTGTCAC AGGGACGTCC 
CGAGTACCTA TGAATGGATT TGCCGAGCTT TATGGTTCCA ATGGTCCTCA GCTGTTTACA 
ATAGAGCAAT GGGGAAGTCC TGAAAAACTG CCCAGAGCTC ACACGTGCTT TAATCGCCTT 
GACTTACCTC CGTATGAAAC CTTCGAAGAT TTACGAGAGA AGCTCCTCAT GGCTGTGGAA 
AACGCCCAAG GCTTCGAGGG GGTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073370.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073432.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBu27444
Clone ID Related Accession (Same CDS sequence)	XM_006073370.1
Accession Version	XM_006073370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785536.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGT GGCCTGATGC TTTTCACCAG GGAGAGTCCC GTATTCTCAG AGTGAAGGTT 
GTTTCTGGAA TTGATCTCGC CAAGAAGGAC ATCTTCGGAG CCAGTGATCC GTATGTGAAA 
CTTTCATTGT ACGTAGCGGA TGAGAATAGG GAACTTGCTT TGGTACAAAC AAAAACAATT 
AAAAAGACGC TGAACCCAAA GTGGAATGAA GAATTTTATT TTAGAGTAAA TCCATCTAAT 
CACAGACTCC TGTTTGAAGT ATTTGATGAA AACAGACTGA CACGGGACGA CTTCCTGGGA 
CAGGTGGACA TACCCCTTAG TCATCTCCCG ACAGAAGACC CAACCATGGA GCGACCCTAC 
ACATTTAAGG ACTTTCTCCT CCGGCCAAGA AGTCATAAAT CTCGGGTTAA GGGATTTTTG 
CGATTGAAAA TGGCCTACAT GCCCAAAAAC GGGGGCCAAG ATGAAGAGAA CAGTGAGCAG 
CGGGATGAGA TGGAGCCCGG GTGGGAAGTC GTTGACTCCA ATGACTCGGC TTCTCAGCAC 
CAGGAGGAGC TGCCCCCGCC TCCGCTGCCC CCCGGGTGGG AAGAAAAGGT GGACAACCTG 
GGCCGCACGT ACTACGTGAA CCACAACAAC CGGACCACCC AGTGGCACCG GCCCAGCCTC 
ATGGACGTGT CGTCCGAGTC CGACAGCAAC ATCCGGCAGA TCAACCAGGA GGCCGCGCAT 
CGCCGCTTCC GCTCGCGCAG ACATATCAGC GAGGACCTGG AGCCGGAGCC CAGCGAGGGA 
GGGGAGGGCC CCGAGCCTTG GGAGACCATT TCAGAGGAAG TGAACATGAG CGGAGACTCG 
CTCAGCCTGG CGCTGCCCCC GCCACCAGCC TCGCCCGTGT CCCGGACGAG CCCCCAGGAG 
CTGTCCGAGG AGCTGAGCAG AAGGCTGCAG GTCACTCCCG ACTCCAACGG CGAGCAGTTT 
GGGTCTTTGA TTCAGCGAGA GCCGTCCTCC CGGCTGAGGT CCTGCAGTGT CACTGATGCA 
GTTGCGGAGC AGGCTCATCT CCCGCCGCCA TCAGTGGCCT ATGTACACAC CACGCCGGGC 
CTGCCCTCGG GCTGGGAAGA GAGGAAAGAT GCTAAGGGAC GCACATACTA TGTCAATCAT 
AACAATCGAA CCACAACTTG GACTCGGCCT ATCGTGCAGC TCGCAGAAGA CGGCGCGTCC 
GGATCCGCCA CAAACAGTAA CAACCACCTA ATCGAGCCTC AGATCCGCCG GCCTCGTAGC 
CTCAGCTCGC CAACAGTAAC TTTATCCGCC CCGCTGGAGG GCGCCAAGGA CTCACCCGTA 
CGTCGGGCTG TGAAAGATAC CCTTTCCAAT CCACAGTCCC CACAGCCATC ACCTTACAAC 
TCCCCCAAAC CACAACACAA AGTCACACAG AGCTTCCTGC CGCCCGGCTG GGAGATGAGG 
ATAGCGCCCA ACGGCCGGCC CTTCTTCATT GACCACAACA CAAAGACCAC GACGTGGGAA 
GATCCACGCC TGAAGTTTCC AGTACACATG CGGTCAAAGG CATCCTTAAA CCCCAATGAC 
CTTGGCCCTC TTCCTCCTGG CTGGGAAGAA AGGATCCACT TGGATGGCCG GACGTTTTAC 
ATTGACCACA ATAGCAAAAT TACTCAGTGG GAAGACCCAA GACTGCAGAA CCCAGCTATT 
ACTGGTCCGG CTGTTCCTTA CTCCAGAGAA TTTAAGCAGA AATATGACTA CTTTAGAAAG 
AAATTAAAGA AACCTGCGGA TATTCCAAAT AGGTTTGAAA TGAAACTTCA CAGAAATAAC 
ATATTTGAAG AATCCTATCG GAGAATCATG TCTGTGAAAA GACCAGATGT CCTGAAAGCT 
AGGCTGTGGA TTGAGTTTGA ATCGGAGAAA GGTCTGGACT ACGGGGGCGT GGCCAGAGAG 
TGGTTCTTCC TACTGTCCAA GGAGATGTTC AACCCTTACT ACGGCCTCTT CGAGTACTCG 
GCAACGGACA ACTACACACT TCAGATCAAC CCCAATTCAG GCCTCTGTAA TGAGGACCAT 
CTGTCCTATT TCACCTTCAT TGGAAGAGTT GCCGGGCTGG CAGTATTTCA TGGGAAACTT 
CTAGATGGTT TCTTCATTCG ACCGTTTTAT AAAATGATGC TGGGAAAGCA GATAACTCTG 
AATGACATGG AATCTGTGGA TAGCGAATAT TACAACTCTT TGAAATGGAT CTTAGAAAAT 
GACCCAACTG AACTGGACCT TATGTTCTGC ATTGATGAAG AGAACTTTGG GCAGACCTAT 
CAAGTGGATC TGAAGCCCAA CGGGTCAGAA ATAATGGTAA CAAATGAAAA CAAGAGAGAG 
TACATCGACC TCGTTATCCA GTGGAGGTTC GTGAACAGAG TCCAGAAGCA GATGAATGCC 
TTCCTGGAGG GATTCACGGA ACTGCTCCCT ATTGATTTGA TTAAAATCTT TGATGAAAAT 
GAACTGGAGT TGCTGATGTG CGGCCTTGGC GATGTGGATG TGAACGACTG GCGACAACAT 
TCTATTTACA AGAATGGCTA CTGCCCGAAC CACCCCGTGA TCCAGTGGTT CTGGAAGGCT 
GTGCTGCTGA TGGACGCCGA GAGGCGCATC CGGTTACTGC AGTTTGTCAC AGGGACGTCC 
CGAGTACCTA TGAATGGATT TGCCGAGCTT TATGGTTCCA ATGGTCCTCA GCTGTTTACA 
ATAGAGCAAT GGGGAAGTCC TGAAAAACTG CCCAGAGCTC ACACGTGCTT TAATCGCCTT 
GACTTACCTC CGTATGAAAC CTTCGAAGAT TTACGAGAGA AGCTCCTCAT GGCTGTGGAA 
AACGCCCAAG GCTTCGAGGG GGTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073432.1
CDS	1..2850
Translation	MEPWPDAFHQGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073370.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073370.1

ORF Insert Sequence:

ATGGAGCCGT GGCCTGATGC TTTTCACCAG GGAGAGTCCC GTATTCTCAG AGTGAAGGTT 
GTTTCTGGAA TTGATCTCGC CAAGAAGGAC ATCTTCGGAG CCAGTGATCC GTATGTGAAA 
CTTTCATTGT ACGTAGCGGA TGAGAATAGG GAACTTGCTT TGGTACAAAC AAAAACAATT 
AAAAAGACGC TGAACCCAAA GTGGAATGAA GAATTTTATT TTAGAGTAAA TCCATCTAAT 
CACAGACTCC TGTTTGAAGT ATTTGATGAA AACAGACTGA CACGGGACGA CTTCCTGGGA 
CAGGTGGACA TACCCCTTAG TCATCTCCCG ACAGAAGACC CAACCATGGA GCGACCCTAC 
ACATTTAAGG ACTTTCTCCT CCGGCCAAGA AGTCATAAAT CTCGGGTTAA GGGATTTTTG 
CGATTGAAAA TGGCCTACAT GCCCAAAAAC GGGGGCCAAG ATGAAGAGAA CAGTGAGCAG 
CGGGATGAGA TGGAGCCCGG GTGGGAAGTC GTTGACTCCA ATGACTCGGC TTCTCAGCAC 
CAGGAGGAGC TGCCCCCGCC TCCGCTGCCC CCCGGGTGGG AAGAAAAGGT GGACAACCTG 
GGCCGCACGT ACTACGTGAA CCACAACAAC CGGACCACCC AGTGGCACCG GCCCAGCCTC 
ATGGACGTGT CGTCCGAGTC CGACAGCAAC ATCCGGCAGA TCAACCAGGA GGCCGCGCAT 
CGCCGCTTCC GCTCGCGCAG ACATATCAGC GAGGACCTGG AGCCGGAGCC CAGCGAGGGA 
GGGGAGGGCC CCGAGCCTTG GGAGACCATT TCAGAGGAAG TGAACATGAG CGGAGACTCG 
CTCAGCCTGG CGCTGCCCCC GCCACCAGCC TCGCCCGTGT CCCGGACGAG CCCCCAGGAG 
CTGTCCGAGG AGCTGAGCAG AAGGCTGCAG GTCACTCCCG ACTCCAACGG CGAGCAGTTT 
GGGTCTTTGA TTCAGCGAGA GCCGTCCTCC CGGCTGAGGT CCTGCAGTGT CACTGATGCA 
GTTGCGGAGC AGGCTCATCT CCCGCCGCCA TCAGTGGCCT ATGTACACAC CACGCCGGGC 
CTGCCCTCGG GCTGGGAAGA GAGGAAAGAT GCTAAGGGAC GCACATACTA TGTCAATCAT 
AACAATCGAA CCACAACTTG GACTCGGCCT ATCGTGCAGC TCGCAGAAGA CGGCGCGTCC 
GGATCCGCCA CAAACAGTAA CAACCACCTA ATCGAGCCTC AGATCCGCCG GCCTCGTAGC 
CTCAGCTCGC CAACAGTAAC TTTATCCGCC CCGCTGGAGG GCGCCAAGGA CTCACCCGTA 
CGTCGGGCTG TGAAAGATAC CCTTTCCAAT CCACAGTCCC CACAGCCATC ACCTTACAAC 
TCCCCCAAAC CACAACACAA AGTCACACAG AGCTTCCTGC CGCCCGGCTG GGAGATGAGG 
ATAGCGCCCA ACGGCCGGCC CTTCTTCATT GACCACAACA CAAAGACCAC GACGTGGGAA 
GATCCACGCC TGAAGTTTCC AGTACACATG CGGTCAAAGG CATCCTTAAA CCCCAATGAC 
CTTGGCCCTC TTCCTCCTGG CTGGGAAGAA AGGATCCACT TGGATGGCCG GACGTTTTAC 
ATTGACCACA ATAGCAAAAT TACTCAGTGG GAAGACCCAA GACTGCAGAA CCCAGCTATT 
ACTGGTCCGG CTGTTCCTTA CTCCAGAGAA TTTAAGCAGA AATATGACTA CTTTAGAAAG 
AAATTAAAGA AACCTGCGGA TATTCCAAAT AGGTTTGAAA TGAAACTTCA CAGAAATAAC 
ATATTTGAAG AATCCTATCG GAGAATCATG TCTGTGAAAA GACCAGATGT CCTGAAAGCT 
AGGCTGTGGA TTGAGTTTGA ATCGGAGAAA GGTCTGGACT ACGGGGGCGT GGCCAGAGAG 
TGGTTCTTCC TACTGTCCAA GGAGATGTTC AACCCTTACT ACGGCCTCTT CGAGTACTCG 
GCAACGGACA ACTACACACT TCAGATCAAC CCCAATTCAG GCCTCTGTAA TGAGGACCAT 
CTGTCCTATT TCACCTTCAT TGGAAGAGTT GCCGGGCTGG CAGTATTTCA TGGGAAACTT 
CTAGATGGTT TCTTCATTCG ACCGTTTTAT AAAATGATGC TGGGAAAGCA GATAACTCTG 
AATGACATGG AATCTGTGGA TAGCGAATAT TACAACTCTT TGAAATGGAT CTTAGAAAAT 
GACCCAACTG AACTGGACCT TATGTTCTGC ATTGATGAAG AGAACTTTGG GCAGACCTAT 
CAAGTGGATC TGAAGCCCAA CGGGTCAGAA ATAATGGTAA CAAATGAAAA CAAGAGAGAG 
TACATCGACC TCGTTATCCA GTGGAGGTTC GTGAACAGAG TCCAGAAGCA GATGAATGCC 
TTCCTGGAGG GATTCACGGA ACTGCTCCCT ATTGATTTGA TTAAAATCTT TGATGAAAAT 
GAACTGGAGT TGCTGATGTG CGGCCTTGGC GATGTGGATG TGAACGACTG GCGACAACAT 
TCTATTTACA AGAATGGCTA CTGCCCGAAC CACCCCGTGA TCCAGTGGTT CTGGAAGGCT 
GTGCTGCTGA TGGACGCCGA GAGGCGCATC CGGTTACTGC AGTTTGTCAC AGGGACGTCC 
CGAGTACCTA TGAATGGATT TGCCGAGCTT TATGGTTCCA ATGGTCCTCA GCTGTTTACA 
ATAGAGCAAT GGGGAAGTCC TGAAAAACTG CCCAGAGCTC ACACGTGCTT TAATCGCCTT 
GACTTACCTC CGTATGAAAC CTTCGAAGAT TTACGAGAGA AGCTCCTCAT GGCTGTGGAA 
AACGCCCAAG GCTTCGAGGG GGTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073372.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073434.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBu27446
Clone ID Related Accession (Same CDS sequence)	XM_006073372.1 , XM_006073373.1
Accession Version	XM_006073372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785536.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
GTTAAGGGAT TTTTGCGATT GAAAATGGCC TACATGCCCA AAAACGGGGG CCAAGATGAA 
GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC 
TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAA 
AAGGTGGACA ACCTGGGCCG CACGTACTAC GTGAACCACA ACAACCGGAC CACCCAGTGG 
CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC 
CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG 
GAGCCCAGCG AGGGAGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC 
ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CAGCCTCGCC CGTGTCCCGG 
ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC 
AACGGCGAGC AGTTTGGGTC TTTGATTCAG CGAGAGCCGT CCTCCCGGCT GAGGTCCTGC 
AGTGTCACTG ATGCAGTTGC GGAGCAGGCT CATCTCCCGC CGCCCAGTGC CCCAGCTGGG 
AGAGCACGTT CATCAACTGT CACCGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA 
CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA 
TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCGT GCAGCTCGCA 
GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC 
CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC 
AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG 
CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC 
GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG 
ACCACGACGT GGGAAGATCC ACGCCTGAAG TTTCCAGTAC ACATGCGGTC AAAGGCATCC 
TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT 
GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA 
GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC 
CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC 
TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC 
AGAAATAACA TATTTGAAGA ATCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC 
CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG 
GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC 
GAGTACTCGG CAACGGACAA CTACACACTT CAGATCAACC CCAATTCAGG CCTCTGTAAT 
GAGGACCATC TGTCCTATTT CACCTTCATT GGAAGAGTTG CCGGGCTGGC AGTATTTCAT 
GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG 
ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC 
TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG 
CAGACCTATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC AAATGAAAAC 
AAGAGAGAGT ACATCGACCT CGTTATCCAG TGGAGGTTCG TGAACAGAGT CCAGAAGCAG 
ATGAATGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT 
GATGAAAATG AACTGGAGTT GCTGATGTGC GGCCTTGGCG ATGTGGATGT GAACGACTGG 
CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC 
TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AGGCGCATCC GGTTACTGCA GTTTGTCACA 
GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG 
CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT 
AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG 
GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073434.1
CDS	373..2991
Translation	MERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSAPAGRARSSTVTGGEAPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHSSQVLCGEDDARDSVPSYDSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073372.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073372.1

ORF Insert Sequence:

ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
GTTAAGGGAT TTTTGCGATT GAAAATGGCC TACATGCCCA AAAACGGGGG CCAAGATGAA 
GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC 
TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAA 
AAGGTGGACA ACCTGGGCCG CACGTACTAC GTGAACCACA ACAACCGGAC CACCCAGTGG 
CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC 
CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG 
GAGCCCAGCG AGGGAGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC 
ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CAGCCTCGCC CGTGTCCCGG 
ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC 
AACGGCGAGC AGTTTGGGTC TTTGATTCAG CGAGAGCCGT CCTCCCGGCT GAGGTCCTGC 
AGTGTCACTG ATGCAGTTGC GGAGCAGGCT CATCTCCCGC CGCCCAGTGC CCCAGCTGGG 
AGAGCACGTT CATCAACTGT CACCGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA 
CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA 
TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCGT GCAGCTCGCA 
GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC 
CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC 
AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG 
CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC 
GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG 
ACCACGACGT GGGAAGATCC ACGCCTGAAG TTTCCAGTAC ACATGCGGTC AAAGGCATCC 
TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT 
GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA 
GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC 
CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC 
TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC 
AGAAATAACA TATTTGAAGA ATCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC 
CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG 
GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC 
GAGTACTCGG CAACGGACAA CTACACACTT CAGATCAACC CCAATTCAGG CCTCTGTAAT 
GAGGACCATC TGTCCTATTT CACCTTCATT GGAAGAGTTG CCGGGCTGGC AGTATTTCAT 
GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG 
ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC 
TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG 
CAGACCTATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC AAATGAAAAC 
AAGAGAGAGT ACATCGACCT CGTTATCCAG TGGAGGTTCG TGAACAGAGT CCAGAAGCAG 
ATGAATGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT 
GATGAAAATG AACTGGAGTT GCTGATGTGC GGCCTTGGCG ATGTGGATGT GAACGACTGG 
CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC 
TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AGGCGCATCC GGTTACTGCA GTTTGTCACA 
GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG 
CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT 
AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG 
GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073373.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073435.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA.

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CloneID	OBu27446
Clone ID Related Accession (Same CDS sequence)	XM_006073372.1 , XM_006073373.1
Accession Version	XM_006073373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785536.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
GTTAAGGGAT TTTTGCGATT GAAAATGGCC TACATGCCCA AAAACGGGGG CCAAGATGAA 
GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC 
TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAA 
AAGGTGGACA ACCTGGGCCG CACGTACTAC GTGAACCACA ACAACCGGAC CACCCAGTGG 
CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC 
CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG 
GAGCCCAGCG AGGGAGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC 
ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CAGCCTCGCC CGTGTCCCGG 
ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC 
AACGGCGAGC AGTTTGGGTC TTTGATTCAG CGAGAGCCGT CCTCCCGGCT GAGGTCCTGC 
AGTGTCACTG ATGCAGTTGC GGAGCAGGCT CATCTCCCGC CGCCCAGTGC CCCAGCTGGG 
AGAGCACGTT CATCAACTGT CACCGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA 
CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA 
TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCGT GCAGCTCGCA 
GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC 
CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC 
AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG 
CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC 
GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG 
ACCACGACGT GGGAAGATCC ACGCCTGAAG TTTCCAGTAC ACATGCGGTC AAAGGCATCC 
TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT 
GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA 
GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC 
CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC 
TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC 
AGAAATAACA TATTTGAAGA ATCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC 
CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG 
GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC 
GAGTACTCGG CAACGGACAA CTACACACTT CAGATCAACC CCAATTCAGG CCTCTGTAAT 
GAGGACCATC TGTCCTATTT CACCTTCATT GGAAGAGTTG CCGGGCTGGC AGTATTTCAT 
GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG 
ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC 
TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG 
CAGACCTATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC AAATGAAAAC 
AAGAGAGAGT ACATCGACCT CGTTATCCAG TGGAGGTTCG TGAACAGAGT CCAGAAGCAG 
ATGAATGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT 
GATGAAAATG AACTGGAGTT GCTGATGTGC GGCCTTGGCG ATGTGGATGT GAACGACTGG 
CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC 
TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AGGCGCATCC GGTTACTGCA GTTTGTCACA 
GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG 
CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT 
AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG 
GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073435.1
CDS	270..2888
Translation	MERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSAPAGRARSSTVTGGEAPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHSSQVLCGEDDARDSVPSYDSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073373.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073373.1

ORF Insert Sequence:

ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
GTTAAGGGAT TTTTGCGATT GAAAATGGCC TACATGCCCA AAAACGGGGG CCAAGATGAA 
GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC 
TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAA 
AAGGTGGACA ACCTGGGCCG CACGTACTAC GTGAACCACA ACAACCGGAC CACCCAGTGG 
CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC 
CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG 
GAGCCCAGCG AGGGAGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC 
ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CAGCCTCGCC CGTGTCCCGG 
ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC 
AACGGCGAGC AGTTTGGGTC TTTGATTCAG CGAGAGCCGT CCTCCCGGCT GAGGTCCTGC 
AGTGTCACTG ATGCAGTTGC GGAGCAGGCT CATCTCCCGC CGCCCAGTGC CCCAGCTGGG 
AGAGCACGTT CATCAACTGT CACCGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA 
CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA 
TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCGT GCAGCTCGCA 
GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC 
CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC 
AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG 
CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC 
GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG 
ACCACGACGT GGGAAGATCC ACGCCTGAAG TTTCCAGTAC ACATGCGGTC AAAGGCATCC 
TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT 
GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA 
GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC 
CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC 
TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC 
AGAAATAACA TATTTGAAGA ATCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC 
CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG 
GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC 
GAGTACTCGG CAACGGACAA CTACACACTT CAGATCAACC CCAATTCAGG CCTCTGTAAT 
GAGGACCATC TGTCCTATTT CACCTTCATT GGAAGAGTTG CCGGGCTGGC AGTATTTCAT 
GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG 
ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC 
TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG 
CAGACCTATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC AAATGAAAAC 
AAGAGAGAGT ACATCGACCT CGTTATCCAG TGGAGGTTCG TGAACAGAGT CCAGAAGCAG 
ATGAATGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT 
GATGAAAATG AACTGGAGTT GCTGATGTGC GGCCTTGGCG ATGTGGATGT GAACGACTGG 
CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC 
TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AGGCGCATCC GGTTACTGCA GTTTGTCACA 
GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG 
CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT 
AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG 
GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073368.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073430.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBu58355
Clone ID Related Accession (Same CDS sequence)	XM_006073368.2
Accession Version	XM_006073368.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006073368.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGAGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGGG CGCCAAGGAC 
TCACCCGTAC GTCGGGCTGT GAAAGATACC CTTTCCAATC CACAGTCCCC ACAGCCATCA 
CCTTACAACT CCCCCAAACC ACAACACAAA GTCACACAGA GCTTCCTGCC GCCCGGCTGG 
GAGATGAGGA TAGCGCCCAA CGGCCGGCCC TTCTTCATTG ACCACAACAC AAAGACCACG 
ACGTGGGAAG ATCCACGCCT GAAGTTTCCA GTACACATGC GGTCAAAGGC ATCCTTAAAC 
CCCAATGACC TTGGCCCTCT TCCTCCTGGC TGGGAAGAAA GGATCCACTT GGATGGCCGG 
ACGTTTTACA TTGACCACAG CAGCCAGGTG TTATGTGGCG AGGACGATGC CAGAGACTCA 
GTGCCCTCGT ACGATAGCAA AATTACTCAG TGGGAAGACC CAAGACTGCA GAACCCAGCT 
ATTACTGGTC CGGCTGTTCC TTACTCCAGA GAATTTAAGC AGAAATATGA CTACTTTAGA 
AAGAAATTAA AGAAACCTGC GGATATTCCA AATAGGTTTG AAATGAAACT TCACAGAAAT 
AACATATTTG AAGAATCCTA TCGGAGAATC ATGTCTGTGA AAAGACCAGA TGTCCTGAAA 
GCTAGGCTGT GGATTGAGTT TGAATCGGAG AAAGGTCTGG ACTACGGGGG CGTGGCCAGA 
GAGTGGTTCT TCCTACTGTC CAAGGAGATG TTCAACCCTT ACTACGGCCT CTTCGAGTAC 
TCGGCAACGG ACAACTACAC ACTTCAGATC AACCCCAATT CAGGCCTCTG TAATGAGGAC 
CATCTGTCCT ATTTCACCTT CATTGGAAGA GTTGCCGGGC TGGCAGTATT TCATGGGAAA 
CTTCTAGATG GTTTCTTCAT TCGACCGTTT TATAAAATGA TGCTGGGAAA GCAGATAACT 
CTGAATGACA TGGAATCTGT GGATAGCGAA TATTACAACT CTTTGAAATG GATCTTAGAA 
AATGACCCAA CTGAACTGGA CCTTATGTTC TGCATTGATG AAGAGAACTT TGGGCAGACC 
TATCAAGTGG ATCTGAAGCC CAACGGGTCA GAAATAATGG TAACAAATGA AAACAAGAGA 
GAGTACATCG ACCTCGTTAT CCAGTGGAGG TTCGTGAACA GAGTCCAGAA GCAGATGAAT 
GCCTTCCTGG AGGGATTCAC GGAACTGCTC CCTATTGATT TGATTAAAAT CTTTGATGAA 
AATGAACTGG AGTTGCTGAT GTGCGGCCTT GGCGATGTGG ATGTGAACGA CTGGCGACAA 
CATTCTATTT ACAAGAATGG CTACTGCCCG AACCACCCCG TGATCCAGTG GTTCTGGAAG 
GCTGTGCTGC TGATGGACGC CGAGAGGCGC ATCCGGTTAC TGCAGTTTGT CACAGGGACG 
TCCCGAGTAC CTATGAATGG ATTTGCCGAG CTTTATGGTT CCAATGGTCC TCAGCTGTTT 
ACAATAGAGC AATGGGGAAG TCCTGAAAAA CTGCCCAGAG CTCACACGTG CTTTAATCGC 
CTTGACTTAC CTCCGTATGA AACCTTCGAA GATTTACGAG AGAAGCTCCT CATGGCTGTG 
GAAAACGCCC AAGGCTTCGA GGGGGTGGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073430.1
CDS	85..2997
Translation	MPSGAEGQAGPAAAAPEPRRWSLQHVPDASGSSGKRCFVFQLQQPPASGGGPAPSGDFNFGFTGTKGDRLVRYPRIRLERSSSYPTQPRAELGSPPEERGHPDPEAPLRGRRVAPDAHRTEAVAERSPQGQGCTFKIRQDQNAGQQHFRILVTRGPEEAPQSPEEDAAAAGPASTGAGAPTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHSSQVLCGEDDARDSVPSYDSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073368.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073368.2

ORF Insert Sequence:

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGAGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGGG CGCCAAGGAC 
TCACCCGTAC GTCGGGCTGT GAAAGATACC CTTTCCAATC CACAGTCCCC ACAGCCATCA 
CCTTACAACT CCCCCAAACC ACAACACAAA GTCACACAGA GCTTCCTGCC GCCCGGCTGG 
GAGATGAGGA TAGCGCCCAA CGGCCGGCCC TTCTTCATTG ACCACAACAC AAAGACCACG 
ACGTGGGAAG ATCCACGCCT GAAGTTTCCA GTACACATGC GGTCAAAGGC ATCCTTAAAC 
CCCAATGACC TTGGCCCTCT TCCTCCTGGC TGGGAAGAAA GGATCCACTT GGATGGCCGG 
ACGTTTTACA TTGACCACAG CAGCCAGGTG TTATGTGGCG AGGACGATGC CAGAGACTCA 
GTGCCCTCGT ACGATAGCAA AATTACTCAG TGGGAAGACC CAAGACTGCA GAACCCAGCT 
ATTACTGGTC CGGCTGTTCC TTACTCCAGA GAATTTAAGC AGAAATATGA CTACTTTAGA 
AAGAAATTAA AGAAACCTGC GGATATTCCA AATAGGTTTG AAATGAAACT TCACAGAAAT 
AACATATTTG AAGAATCCTA TCGGAGAATC ATGTCTGTGA AAAGACCAGA TGTCCTGAAA 
GCTAGGCTGT GGATTGAGTT TGAATCGGAG AAAGGTCTGG ACTACGGGGG CGTGGCCAGA 
GAGTGGTTCT TCCTACTGTC CAAGGAGATG TTCAACCCTT ACTACGGCCT CTTCGAGTAC 
TCGGCAACGG ACAACTACAC ACTTCAGATC AACCCCAATT CAGGCCTCTG TAATGAGGAC 
CATCTGTCCT ATTTCACCTT CATTGGAAGA GTTGCCGGGC TGGCAGTATT TCATGGGAAA 
CTTCTAGATG GTTTCTTCAT TCGACCGTTT TATAAAATGA TGCTGGGAAA GCAGATAACT 
CTGAATGACA TGGAATCTGT GGATAGCGAA TATTACAACT CTTTGAAATG GATCTTAGAA 
AATGACCCAA CTGAACTGGA CCTTATGTTC TGCATTGATG AAGAGAACTT TGGGCAGACC 
TATCAAGTGG ATCTGAAGCC CAACGGGTCA GAAATAATGG TAACAAATGA AAACAAGAGA 
GAGTACATCG ACCTCGTTAT CCAGTGGAGG TTCGTGAACA GAGTCCAGAA GCAGATGAAT 
GCCTTCCTGG AGGGATTCAC GGAACTGCTC CCTATTGATT TGATTAAAAT CTTTGATGAA 
AATGAACTGG AGTTGCTGAT GTGCGGCCTT GGCGATGTGG ATGTGAACGA CTGGCGACAA 
CATTCTATTT ACAAGAATGG CTACTGCCCG AACCACCCCG TGATCCAGTG GTTCTGGAAG 
GCTGTGCTGC TGATGGACGC CGAGAGGCGC ATCCGGTTAC TGCAGTTTGT CACAGGGACG 
TCCCGAGTAC CTATGAATGG ATTTGCCGAG CTTTATGGTT CCAATGGTCC TCAGCTGTTT 
ACAATAGAGC AATGGGGAAG TCCTGAAAAA CTGCCCAGAG CTCACACGTG CTTTAATCGC 
CTTGACTTAC CTCCGTATGA AACCTTCGAA GATTTACGAG AGAAGCTCCT CATGGCTGTG 
GAAAACGCCC AAGGCTTCGA GGGGGTGGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073371.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073433.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBu58356
Clone ID Related Accession (Same CDS sequence)	XM_006073371.2
Accession Version	XM_006073371.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006073371.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGAGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGGA AGATCCACGC 
CTGAAGTTTC CAGTACACAT GCGGTCAAAG GCATCCTTAA ACCCCAATGA CCTTGGCCCT 
CTTCCTCCTG GCTGGGAAGA AAGGATCCAC TTGGATGGCC GGACGTTTTA CATTGACCAC 
AGCAGCCAGG TGTTATGTGG CGAGGACGAT GCCAGAGACT CAGTGCCCTC GTACGATAGC 
AAAATTACTC AGTGGGAAGA CCCAAGACTG CAGAACCCAG CTATTACTGG TCCGGCTGTT 
CCTTACTCCA GAGAATTTAA GCAGAAATAT GACTACTTTA GAAAGAAATT AAAGAAACCT 
GCGGATATTC CAAATAGGTT TGAAATGAAA CTTCACAGAA ATAACATATT TGAAGAATCC 
TATCGGAGAA TCATGTCTGT GAAAAGACCA GATGTCCTGA AAGCTAGGCT GTGGATTGAG 
TTTGAATCGG AGAAAGGTCT GGACTACGGG GGCGTGGCCA GAGAGTGGTT CTTCCTACTG 
TCCAAGGAGA TGTTCAACCC TTACTACGGC CTCTTCGAGT ACTCGGCAAC GGACAACTAC 
ACACTTCAGA TCAACCCCAA TTCAGGCCTC TGTAATGAGG ACCATCTGTC CTATTTCACC 
TTCATTGGAA GAGTTGCCGG GCTGGCAGTA TTTCATGGGA AACTTCTAGA TGGTTTCTTC 
ATTCGACCGT TTTATAAAAT GATGCTGGGA AAGCAGATAA CTCTGAATGA CATGGAATCT 
GTGGATAGCG AATATTACAA CTCTTTGAAA TGGATCTTAG AAAATGACCC AACTGAACTG 
GACCTTATGT TCTGCATTGA TGAAGAGAAC TTTGGGCAGA CCTATCAAGT GGATCTGAAG 
CCCAACGGGT CAGAAATAAT GGTAACAAAT GAAAACAAGA GAGAGTACAT CGACCTCGTT 
ATCCAGTGGA GGTTCGTGAA CAGAGTCCAG AAGCAGATGA ATGCCTTCCT GGAGGGATTC 
ACGGAACTGC TCCCTATTGA TTTGATTAAA ATCTTTGATG AAAATGAACT GGAGTTGCTG 
ATGTGCGGCC TTGGCGATGT GGATGTGAAC GACTGGCGAC AACATTCTAT TTACAAGAAT 
GGCTACTGCC CGAACCACCC CGTGATCCAG TGGTTCTGGA AGGCTGTGCT GCTGATGGAC 
GCCGAGAGGC GCATCCGGTT ACTGCAGTTT GTCACAGGGA CGTCCCGAGT ACCTATGAAT 
GGATTTGCCG AGCTTTATGG TTCCAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGA 
AGTCCTGAAA AACTGCCCAG AGCTCACACG TGCTTTAATC GCCTTGACTT ACCTCCGTAT 
GAAACCTTCG AAGATTTACG AGAGAAGCTC CTCATGGCTG TGGAAAACGC CCAAGGCTTC 
GAGGGGGTGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073433.1
CDS	85..2799
Translation	MPSGAEGQAGPAAAAPEPRRWSLQHVPDASGSSGKRCFVFQLQQPPASGGGPAPSGDFNFGFTGTKGDRLVRYPRIRLERSSSYPTQPRAELGSPPEERGHPDPEAPLRGRRVAPDAHRTEAVAERSPQGQGCTFKIRQDQNAGQQHFRILVTRGPEEAPQSPEEDAAAAGPASTGAGAPTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHSSQVLCGEDDARDSVPSYDSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073371.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073371.2

ORF Insert Sequence:

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGAGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGGA AGATCCACGC 
CTGAAGTTTC CAGTACACAT GCGGTCAAAG GCATCCTTAA ACCCCAATGA CCTTGGCCCT 
CTTCCTCCTG GCTGGGAAGA AAGGATCCAC TTGGATGGCC GGACGTTTTA CATTGACCAC 
AGCAGCCAGG TGTTATGTGG CGAGGACGAT GCCAGAGACT CAGTGCCCTC GTACGATAGC 
AAAATTACTC AGTGGGAAGA CCCAAGACTG CAGAACCCAG CTATTACTGG TCCGGCTGTT 
CCTTACTCCA GAGAATTTAA GCAGAAATAT GACTACTTTA GAAAGAAATT AAAGAAACCT 
GCGGATATTC CAAATAGGTT TGAAATGAAA CTTCACAGAA ATAACATATT TGAAGAATCC 
TATCGGAGAA TCATGTCTGT GAAAAGACCA GATGTCCTGA AAGCTAGGCT GTGGATTGAG 
TTTGAATCGG AGAAAGGTCT GGACTACGGG GGCGTGGCCA GAGAGTGGTT CTTCCTACTG 
TCCAAGGAGA TGTTCAACCC TTACTACGGC CTCTTCGAGT ACTCGGCAAC GGACAACTAC 
ACACTTCAGA TCAACCCCAA TTCAGGCCTC TGTAATGAGG ACCATCTGTC CTATTTCACC 
TTCATTGGAA GAGTTGCCGG GCTGGCAGTA TTTCATGGGA AACTTCTAGA TGGTTTCTTC 
ATTCGACCGT TTTATAAAAT GATGCTGGGA AAGCAGATAA CTCTGAATGA CATGGAATCT 
GTGGATAGCG AATATTACAA CTCTTTGAAA TGGATCTTAG AAAATGACCC AACTGAACTG 
GACCTTATGT TCTGCATTGA TGAAGAGAAC TTTGGGCAGA CCTATCAAGT GGATCTGAAG 
CCCAACGGGT CAGAAATAAT GGTAACAAAT GAAAACAAGA GAGAGTACAT CGACCTCGTT 
ATCCAGTGGA GGTTCGTGAA CAGAGTCCAG AAGCAGATGA ATGCCTTCCT GGAGGGATTC 
ACGGAACTGC TCCCTATTGA TTTGATTAAA ATCTTTGATG AAAATGAACT GGAGTTGCTG 
ATGTGCGGCC TTGGCGATGT GGATGTGAAC GACTGGCGAC AACATTCTAT TTACAAGAAT 
GGCTACTGCC CGAACCACCC CGTGATCCAG TGGTTCTGGA AGGCTGTGCT GCTGATGGAC 
GCCGAGAGGC GCATCCGGTT ACTGCAGTTT GTCACAGGGA CGTCCCGAGT ACCTATGAAT 
GGATTTGCCG AGCTTTATGG TTCCAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGA 
AGTCCTGAAA AACTGCCCAG AGCTCACACG TGCTTTAATC GCCTTGACTT ACCTCCGTAT 
GAAACCTTCG AAGATTTACG AGAGAAGCTC CTCATGGCTG TGGAAAACGC CCAAGGCTTC 
GAGGGGGTGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025273167.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128952.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBu58357
Clone ID Related Accession (Same CDS sequence)	XM_025273167.1
Accession Version	XM_025273167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCG GGCTCGGGGA GCCGGTCTAC GGACTTTCGG AAGAAGAGGG AGAGTCCCGT 
ATTCTCAGAG TGAAGGTTGT TTCTGGAATT GATCTCGCCA AGAAGGACAT CTTCGGAGCC 
AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGGGA ACTTGCTTTG 
GTACAAACAA AAACAATTAA AAAGACGCTG AACCCAAAGT GGAATGAAGA ATTTTATTTT 
AGAGTAAATC CATCTAATCA CAGACTCCTG TTTGAAGTAT TTGATGAAAA CAGACTGACA 
CGGGACGACT TCCTGGGACA GGTGGACATA CCCCTTAGTC ATCTCCCGAC AGAAGACCCA 
ACCATGGAGC GACCCTACAC ATTTAAGGAC TTTCTCCTCC GGCCAAGAAG TCATAAATCT 
CGGGTTAAGG GATTTTTGCG ATTGAAAATG GCCTACATGC CCAAAAACGG AGGCCAAGAT 
GAAGAGAACA GTGAGCAGCG GGATGAGATG GAGCCCGGGT GGGAAGTCGT TGACTCCAAT 
GACTCGGCTT CTCAGCACCA GGAGGAGCTG CCCCCGCCTC CGCTGCCCCC CGGGTGGGAA 
GAAAAGGTGG ACAACCTGGG CCGCACGTAC TACGTGAACC ACAACAACCG GACCACCCAG 
TGGCACCGGC CCAGCCTCAT GGACGTGTCG TCCGAGTCCG ACAGCAACAT CCGGCAGATC 
AACCAGGAGG CCGCGCATCG CCGCTTCCGC TCGCGCAGAC ATATCAGCGA GGACCTGGAG 
CCGGAGCCCA GCGAGGGAGG GGAGGGCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG 
AACATGAGCG GAGACTCGCT CAGCCTGGCG CTGCCCCCGC CACCAGCCTC GCCCGTGTCC 
CGGACGAGCC CCCAGGAGCT GTCCGAGGAG CTGAGCAGAA GGCTGCAGGT CACTCCCGAC 
TCCAACGGCG AGCAGTTTGG GTCTTTGATT CAGCGAGAGC CGTCCTCCCG GCTGAGGTCC 
TGCAGTGTCA CTGATGCAGT TGCGGAGCAG GCTCATCTCC CGCCGCCCAG TGCCCCAGCT 
GGGAGAGCAC GTTCATCAAC TGTCACCGGT GGTGAGGCGC CAACGCCATC AGTGGCCTAT 
GTACACACCA CGCCGGGCCT GCCCTCGGGC TGGGAAGAGA GGAAAGATGC TAAGGGACGC 
ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGGCCTAT CGTGCAGCTC 
GCAGAAGACG GCGCGTCCGG ATCCGCCACA AACAGTAACA ACCACCTAAT CGAGCCTCAG 
ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCCGCCCC GCTGGAGGGC 
GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGATACCC TTTCCAATCC ACAGTCCCCA 
CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCCTGCCG 
CCCGGCTGGG AGATGAGGAT AGCGCCCAAC GGCCGGCCCT TCTTCATTGA CCACAACACA 
AAGACCACGA CGTGGGAAGA TCCACGCCTG AAGTTTCCAG TACACATGCG GTCAAAGGCA 
TCCTTAAACC CCAATGACCT TGGCCCTCTT CCTCCTGGCT GGGAAGAAAG GATCCACTTG 
GATGGCCGGA CGTTTTACAT TGACCACAGC AGCCAGGTGT TATGTGGCGA GGACGATGCC 
AGAGACTCAG TGCCCTCGTA CGATAGCAAA ATTACTCAGT GGGAAGACCC AAGACTGCAG 
AACCCAGCTA TTACTGGTCC GGCTGTTCCT TACTCCAGAG AATTTAAGCA GAAATATGAC 
TACTTTAGAA AGAAATTAAA GAAACCTGCG GATATTCCAA ATAGGTTTGA AATGAAACTT 
CACAGAAATA ACATATTTGA AGAATCCTAT CGGAGAATCA TGTCTGTGAA AAGACCAGAT 
GTCCTGAAAG CTAGGCTGTG GATTGAGTTT GAATCGGAGA AAGGTCTGGA CTACGGGGGC 
GTGGCCAGAG AGTGGTTCTT CCTACTGTCC AAGGAGATGT TCAACCCTTA CTACGGCCTC 
TTCGAGTACT CGGCAACGGA CAACTACACA CTTCAGATCA ACCCCAATTC AGGCCTCTGT 
AATGAGGACC ATCTGTCCTA TTTCACCTTC ATTGGAAGAG TTGCCGGGCT GGCAGTATTT 
CATGGGAAAC TTCTAGATGG TTTCTTCATT CGACCGTTTT ATAAAATGAT GCTGGGAAAG 
CAGATAACTC TGAATGACAT GGAATCTGTG GATAGCGAAT ATTACAACTC TTTGAAATGG 
ATCTTAGAAA ATGACCCAAC TGAACTGGAC CTTATGTTCT GCATTGATGA AGAGAACTTT 
GGGCAGACCT ATCAAGTGGA TCTGAAGCCC AACGGGTCAG AAATAATGGT AACAAATGAA 
AACAAGAGAG AGTACATCGA CCTCGTTATC CAGTGGAGGT TCGTGAACAG AGTCCAGAAG 
CAGATGAATG CCTTCCTGGA GGGATTCACG GAACTGCTCC CTATTGATTT GATTAAAATC 
TTTGATGAAA ATGAACTGGA GTTGCTGATG TGCGGCCTTG GCGATGTGGA TGTGAACGAC 
TGGCGACAAC ATTCTATTTA CAAGAATGGC TACTGCCCGA ACCACCCCGT GATCCAGTGG 
TTCTGGAAGG CTGTGCTGCT GATGGACGCC GAGAGGCGCA TCCGGTTACT GCAGTTTGTC 
ACAGGGACGT CCCGAGTACC TATGAATGGA TTTGCCGAGC TTTATGGTTC CAATGGTCCT 
CAGCTGTTTA CAATAGAGCA ATGGGGAAGT CCTGAAAAAC TGCCCAGAGC TCACACGTGC 
TTTAATCGCC TTGACTTACC TCCGTATGAA ACCTTCGAAG ATTTACGAGA GAAGCTCCTC 
ATGGCTGTGG AAAACGCCCA AGGCTTCGAG GGGGTGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128952.1
CDS	193..3174
Translation	MATGLGEPVYGLSEEEGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSAPAGRARSSTVTGGEAPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHSSQVLCGEDDARDSVPSYDSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_025273167.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025273167.1

ORF Insert Sequence:

ATGGCGACCG GGCTCGGGGA GCCGGTCTAC GGACTTTCGG AAGAAGAGGG AGAGTCCCGT 
ATTCTCAGAG TGAAGGTTGT TTCTGGAATT GATCTCGCCA AGAAGGACAT CTTCGGAGCC 
AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGGGA ACTTGCTTTG 
GTACAAACAA AAACAATTAA AAAGACGCTG AACCCAAAGT GGAATGAAGA ATTTTATTTT 
AGAGTAAATC CATCTAATCA CAGACTCCTG TTTGAAGTAT TTGATGAAAA CAGACTGACA 
CGGGACGACT TCCTGGGACA GGTGGACATA CCCCTTAGTC ATCTCCCGAC AGAAGACCCA 
ACCATGGAGC GACCCTACAC ATTTAAGGAC TTTCTCCTCC GGCCAAGAAG TCATAAATCT 
CGGGTTAAGG GATTTTTGCG ATTGAAAATG GCCTACATGC CCAAAAACGG AGGCCAAGAT 
GAAGAGAACA GTGAGCAGCG GGATGAGATG GAGCCCGGGT GGGAAGTCGT TGACTCCAAT 
GACTCGGCTT CTCAGCACCA GGAGGAGCTG CCCCCGCCTC CGCTGCCCCC CGGGTGGGAA 
GAAAAGGTGG ACAACCTGGG CCGCACGTAC TACGTGAACC ACAACAACCG GACCACCCAG 
TGGCACCGGC CCAGCCTCAT GGACGTGTCG TCCGAGTCCG ACAGCAACAT CCGGCAGATC 
AACCAGGAGG CCGCGCATCG CCGCTTCCGC TCGCGCAGAC ATATCAGCGA GGACCTGGAG 
CCGGAGCCCA GCGAGGGAGG GGAGGGCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG 
AACATGAGCG GAGACTCGCT CAGCCTGGCG CTGCCCCCGC CACCAGCCTC GCCCGTGTCC 
CGGACGAGCC CCCAGGAGCT GTCCGAGGAG CTGAGCAGAA GGCTGCAGGT CACTCCCGAC 
TCCAACGGCG AGCAGTTTGG GTCTTTGATT CAGCGAGAGC CGTCCTCCCG GCTGAGGTCC 
TGCAGTGTCA CTGATGCAGT TGCGGAGCAG GCTCATCTCC CGCCGCCCAG TGCCCCAGCT 
GGGAGAGCAC GTTCATCAAC TGTCACCGGT GGTGAGGCGC CAACGCCATC AGTGGCCTAT 
GTACACACCA CGCCGGGCCT GCCCTCGGGC TGGGAAGAGA GGAAAGATGC TAAGGGACGC 
ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGGCCTAT CGTGCAGCTC 
GCAGAAGACG GCGCGTCCGG ATCCGCCACA AACAGTAACA ACCACCTAAT CGAGCCTCAG 
ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCCGCCCC GCTGGAGGGC 
GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGATACCC TTTCCAATCC ACAGTCCCCA 
CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCCTGCCG 
CCCGGCTGGG AGATGAGGAT AGCGCCCAAC GGCCGGCCCT TCTTCATTGA CCACAACACA 
AAGACCACGA CGTGGGAAGA TCCACGCCTG AAGTTTCCAG TACACATGCG GTCAAAGGCA 
TCCTTAAACC CCAATGACCT TGGCCCTCTT CCTCCTGGCT GGGAAGAAAG GATCCACTTG 
GATGGCCGGA CGTTTTACAT TGACCACAGC AGCCAGGTGT TATGTGGCGA GGACGATGCC 
AGAGACTCAG TGCCCTCGTA CGATAGCAAA ATTACTCAGT GGGAAGACCC AAGACTGCAG 
AACCCAGCTA TTACTGGTCC GGCTGTTCCT TACTCCAGAG AATTTAAGCA GAAATATGAC 
TACTTTAGAA AGAAATTAAA GAAACCTGCG GATATTCCAA ATAGGTTTGA AATGAAACTT 
CACAGAAATA ACATATTTGA AGAATCCTAT CGGAGAATCA TGTCTGTGAA AAGACCAGAT 
GTCCTGAAAG CTAGGCTGTG GATTGAGTTT GAATCGGAGA AAGGTCTGGA CTACGGGGGC 
GTGGCCAGAG AGTGGTTCTT CCTACTGTCC AAGGAGATGT TCAACCCTTA CTACGGCCTC 
TTCGAGTACT CGGCAACGGA CAACTACACA CTTCAGATCA ACCCCAATTC AGGCCTCTGT 
AATGAGGACC ATCTGTCCTA TTTCACCTTC ATTGGAAGAG TTGCCGGGCT GGCAGTATTT 
CATGGGAAAC TTCTAGATGG TTTCTTCATT CGACCGTTTT ATAAAATGAT GCTGGGAAAG 
CAGATAACTC TGAATGACAT GGAATCTGTG GATAGCGAAT ATTACAACTC TTTGAAATGG 
ATCTTAGAAA ATGACCCAAC TGAACTGGAC CTTATGTTCT GCATTGATGA AGAGAACTTT 
GGGCAGACCT ATCAAGTGGA TCTGAAGCCC AACGGGTCAG AAATAATGGT AACAAATGAA 
AACAAGAGAG AGTACATCGA CCTCGTTATC CAGTGGAGGT TCGTGAACAG AGTCCAGAAG 
CAGATGAATG CCTTCCTGGA GGGATTCACG GAACTGCTCC CTATTGATTT GATTAAAATC 
TTTGATGAAA ATGAACTGGA GTTGCTGATG TGCGGCCTTG GCGATGTGGA TGTGAACGAC 
TGGCGACAAC ATTCTATTTA CAAGAATGGC TACTGCCCGA ACCACCCCGT GATCCAGTGG 
TTCTGGAAGG CTGTGCTGCT GATGGACGCC GAGAGGCGCA TCCGGTTACT GCAGTTTGTC 
ACAGGGACGT CCCGAGTACC TATGAATGGA TTTGCCGAGC TTTATGGTTC CAATGGTCCT 
CAGCTGTTTA CAATAGAGCA ATGGGGAAGT CCTGAAAAAC TGCCCAGAGC TCACACGTGC 
TTTAATCGCC TTGACTTACC TCCGTATGAA ACCTTCGAAG ATTTACGAGA GAAGCTCCTC 
ATGGCTGTGG AAAACGCCCA AGGCTTCGAG GGGGTGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025273168.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128953.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBu58358
Clone ID Related Accession (Same CDS sequence)	XM_025273168.1
Accession Version	XM_025273168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCG GGCTCGGGGA GCCGGTCTAC GGACTTTCGG AAGAAGAGGG AGAGTCCCGT 
ATTCTCAGAG TGAAGGTTGT TTCTGGAATT GATCTCGCCA AGAAGGACAT CTTCGGAGCC 
AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGGGA ACTTGCTTTG 
GTACAAACAA AAACAATTAA AAAGACGCTG AACCCAAAGT GGAATGAAGA ATTTTATTTT 
AGAGTAAATC CATCTAATCA CAGACTCCTG TTTGAAGTAT TTGATGAAAA CAGACTGACA 
CGGGACGACT TCCTGGGACA GGTGGACATA CCCCTTAGTC ATCTCCCGAC AGAAGACCCA 
ACCATGGAGC GACCCTACAC ATTTAAGGAC TTTCTCCTCC GGCCAAGAAG TCATAAATCT 
CGGGTTAAGG GATTTTTGCG ATTGAAAATG GCCTACATGC CCAAAAACGG AGGCCAAGAT 
GAAGAGAACA GTGAGCAGCG GGATGAGATG GAGCCCGGGT GGGAAGTCGT TGACTCCAAT 
GACTCGGCTT CTCAGCACCA GGAGGAGCTG CCCCCGCCTC CGCTGCCCCC CGGGTGGGAA 
GAAAAGGTGG ACAACCTGGG CCGCACGTAC TACGTGAACC ACAACAACCG GACCACCCAG 
TGGCACCGGC CCAGCCTCAT GGACGTGTCG TCCGAGTCCG ACAGCAACAT CCGGCAGATC 
AACCAGGAGG CCGCGCATCG CCGCTTCCGC TCGCGCAGAC ATATCAGCGA GGACCTGGAG 
CCGGAGCCCA GCGAGGGAGG GGAGGGCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG 
AACATGAGCG GAGACTCGCT CAGCCTGGCG CTGCCCCCGC CACCAGCCTC GCCCGTGTCC 
CGGACGAGCC CCCAGGAGCT GTCCGAGGAG CTGAGCAGAA GGCTGCAGGT CACTCCCGAC 
TCCAACGGCG AGCAGTTTGG GTCTTTGATT CAGCGAGAGC CGTCCTCCCG GCTGAGGTCC 
TGCAGTGTCA CTGATGCAGT TGCGGAGCAG GCTCATCTCC CGCCGCCCAG TGCCCCAGCT 
GGGAGAGCAC GTTCATCAAC TGTCACCGGT GGTGAGGCGC CAACGCCATC AGTGGCCTAT 
GTACACACCA CGCCGGGCCT GCCCTCGGGC TGGGAAGAGA GGAAAGATGC TAAGGGACGC 
ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGGCCTAT CGTGCAGCTC 
GCAGAAGACG GCGCGTCCGG ATCCGCCACA AACAGTAACA ACCACCTAAT CGAGCCTCAG 
ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCCGCCCC GCTGGAGGGC 
GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGATACCC TTTCCAATCC ACAGTCCCCA 
CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCCTGCCG 
CCCGGCTGGG AGATGAGGAT AGCGCCCAAC GGCCGGCCCT TCTTCATTGA CCACAACACA 
AAGACCACGA CGTGGGAAGA TCCACGCCTG AAGTTTCCAG TACACATGCG GTCAAAGGCA 
TCCTTAAACC CCAATGACCT TGGCCCTCTT CCTCCTGGCT GGGAAGAAAG GATCCACTTG 
GATGGCCGGA CGTTTTACAT TGACCACAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA 
CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTTCCTTACT CCAGAGAATT TAAGCAGAAA 
TATGACTACT TTAGAAAGAA ATTAAAGAAA CCTGCGGATA TTCCAAATAG GTTTGAAATG 
AAACTTCACA GAAATAACAT ATTTGAAGAA TCCTATCGGA GAATCATGTC TGTGAAAAGA 
CCAGATGTCC TGAAAGCTAG GCTGTGGATT GAGTTTGAAT CGGAGAAAGG TCTGGACTAC 
GGGGGCGTGG CCAGAGAGTG GTTCTTCCTA CTGTCCAAGG AGATGTTCAA CCCTTACTAC 
GGCCTCTTCG AGTACTCGGC AACGGACAAC TACACACTTC AGATCAACCC CAATTCAGGC 
CTCTGTAATG AGGACCATCT GTCCTATTTC ACCTTCATTG GAAGAGTTGC CGGGCTGGCA 
GTATTTCATG GGAAACTTCT AGATGGTTTC TTCATTCGAC CGTTTTATAA AATGATGCTG 
GGAAAGCAGA TAACTCTGAA TGACATGGAA TCTGTGGATA GCGAATATTA CAACTCTTTG 
AAATGGATCT TAGAAAATGA CCCAACTGAA CTGGACCTTA TGTTCTGCAT TGATGAAGAG 
AACTTTGGGC AGACCTATCA AGTGGATCTG AAGCCCAACG GGTCAGAAAT AATGGTAACA 
AATGAAAACA AGAGAGAGTA CATCGACCTC GTTATCCAGT GGAGGTTCGT GAACAGAGTC 
CAGAAGCAGA TGAATGCCTT CCTGGAGGGA TTCACGGAAC TGCTCCCTAT TGATTTGATT 
AAAATCTTTG ATGAAAATGA ACTGGAGTTG CTGATGTGCG GCCTTGGCGA TGTGGATGTG 
AACGACTGGC GACAACATTC TATTTACAAG AATGGCTACT GCCCGAACCA CCCCGTGATC 
CAGTGGTTCT GGAAGGCTGT GCTGCTGATG GACGCCGAGA GGCGCATCCG GTTACTGCAG 
TTTGTCACAG GGACGTCCCG AGTACCTATG AATGGATTTG CCGAGCTTTA TGGTTCCAAT 
GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC 
ACGTGCTTTA ATCGCCTTGA CTTACCTCCG TATGAAACCT TCGAAGATTT ACGAGAGAAG 
CTCCTCATGG CTGTGGAAAA CGCCCAAGGC TTCGAGGGGG TGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128953.1
CDS	189..3116
Translation	MATGLGEPVYGLSEEEGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSAPAGRARSSTVTGGEAPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_025273168.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025273168.1

ORF Insert Sequence:

ATGGCGACCG GGCTCGGGGA GCCGGTCTAC GGACTTTCGG AAGAAGAGGG AGAGTCCCGT 
ATTCTCAGAG TGAAGGTTGT TTCTGGAATT GATCTCGCCA AGAAGGACAT CTTCGGAGCC 
AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGGGA ACTTGCTTTG 
GTACAAACAA AAACAATTAA AAAGACGCTG AACCCAAAGT GGAATGAAGA ATTTTATTTT 
AGAGTAAATC CATCTAATCA CAGACTCCTG TTTGAAGTAT TTGATGAAAA CAGACTGACA 
CGGGACGACT TCCTGGGACA GGTGGACATA CCCCTTAGTC ATCTCCCGAC AGAAGACCCA 
ACCATGGAGC GACCCTACAC ATTTAAGGAC TTTCTCCTCC GGCCAAGAAG TCATAAATCT 
CGGGTTAAGG GATTTTTGCG ATTGAAAATG GCCTACATGC CCAAAAACGG AGGCCAAGAT 
GAAGAGAACA GTGAGCAGCG GGATGAGATG GAGCCCGGGT GGGAAGTCGT TGACTCCAAT 
GACTCGGCTT CTCAGCACCA GGAGGAGCTG CCCCCGCCTC CGCTGCCCCC CGGGTGGGAA 
GAAAAGGTGG ACAACCTGGG CCGCACGTAC TACGTGAACC ACAACAACCG GACCACCCAG 
TGGCACCGGC CCAGCCTCAT GGACGTGTCG TCCGAGTCCG ACAGCAACAT CCGGCAGATC 
AACCAGGAGG CCGCGCATCG CCGCTTCCGC TCGCGCAGAC ATATCAGCGA GGACCTGGAG 
CCGGAGCCCA GCGAGGGAGG GGAGGGCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG 
AACATGAGCG GAGACTCGCT CAGCCTGGCG CTGCCCCCGC CACCAGCCTC GCCCGTGTCC 
CGGACGAGCC CCCAGGAGCT GTCCGAGGAG CTGAGCAGAA GGCTGCAGGT CACTCCCGAC 
TCCAACGGCG AGCAGTTTGG GTCTTTGATT CAGCGAGAGC CGTCCTCCCG GCTGAGGTCC 
TGCAGTGTCA CTGATGCAGT TGCGGAGCAG GCTCATCTCC CGCCGCCCAG TGCCCCAGCT 
GGGAGAGCAC GTTCATCAAC TGTCACCGGT GGTGAGGCGC CAACGCCATC AGTGGCCTAT 
GTACACACCA CGCCGGGCCT GCCCTCGGGC TGGGAAGAGA GGAAAGATGC TAAGGGACGC 
ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGGCCTAT CGTGCAGCTC 
GCAGAAGACG GCGCGTCCGG ATCCGCCACA AACAGTAACA ACCACCTAAT CGAGCCTCAG 
ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCCGCCCC GCTGGAGGGC 
GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGATACCC TTTCCAATCC ACAGTCCCCA 
CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCCTGCCG 
CCCGGCTGGG AGATGAGGAT AGCGCCCAAC GGCCGGCCCT TCTTCATTGA CCACAACACA 
AAGACCACGA CGTGGGAAGA TCCACGCCTG AAGTTTCCAG TACACATGCG GTCAAAGGCA 
TCCTTAAACC CCAATGACCT TGGCCCTCTT CCTCCTGGCT GGGAAGAAAG GATCCACTTG 
GATGGCCGGA CGTTTTACAT TGACCACAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA 
CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTTCCTTACT CCAGAGAATT TAAGCAGAAA 
TATGACTACT TTAGAAAGAA ATTAAAGAAA CCTGCGGATA TTCCAAATAG GTTTGAAATG 
AAACTTCACA GAAATAACAT ATTTGAAGAA TCCTATCGGA GAATCATGTC TGTGAAAAGA 
CCAGATGTCC TGAAAGCTAG GCTGTGGATT GAGTTTGAAT CGGAGAAAGG TCTGGACTAC 
GGGGGCGTGG CCAGAGAGTG GTTCTTCCTA CTGTCCAAGG AGATGTTCAA CCCTTACTAC 
GGCCTCTTCG AGTACTCGGC AACGGACAAC TACACACTTC AGATCAACCC CAATTCAGGC 
CTCTGTAATG AGGACCATCT GTCCTATTTC ACCTTCATTG GAAGAGTTGC CGGGCTGGCA 
GTATTTCATG GGAAACTTCT AGATGGTTTC TTCATTCGAC CGTTTTATAA AATGATGCTG 
GGAAAGCAGA TAACTCTGAA TGACATGGAA TCTGTGGATA GCGAATATTA CAACTCTTTG 
AAATGGATCT TAGAAAATGA CCCAACTGAA CTGGACCTTA TGTTCTGCAT TGATGAAGAG 
AACTTTGGGC AGACCTATCA AGTGGATCTG AAGCCCAACG GGTCAGAAAT AATGGTAACA 
AATGAAAACA AGAGAGAGTA CATCGACCTC GTTATCCAGT GGAGGTTCGT GAACAGAGTC 
CAGAAGCAGA TGAATGCCTT CCTGGAGGGA TTCACGGAAC TGCTCCCTAT TGATTTGATT 
AAAATCTTTG ATGAAAATGA ACTGGAGTTG CTGATGTGCG GCCTTGGCGA TGTGGATGTG 
AACGACTGGC GACAACATTC TATTTACAAG AATGGCTACT GCCCGAACCA CCCCGTGATC 
CAGTGGTTCT GGAAGGCTGT GCTGCTGATG GACGCCGAGA GGCGCATCCG GTTACTGCAG 
TTTGTCACAG GGACGTCCCG AGTACCTATG AATGGATTTG CCGAGCTTTA TGGTTCCAAT 
GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC 
ACGTGCTTTA ATCGCCTTGA CTTACCTCCG TATGAAACCT TCGAAGATTT ACGAGAGAAG 
CTCCTCATGG CTGTGGAAAA CGCCCAAGGC TTCGAGGGGG TGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025273169.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128954.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBu58359
Clone ID Related Accession (Same CDS sequence)	XM_025273169.1
Accession Version	XM_025273169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCG GGCTCGGGGA GCCGGTCTAC GGACTTTCGG AAGAAGAGGG AGAGTCCCGT 
ATTCTCAGAG TGAAGGTTGT TTCTGGAATT GATCTCGCCA AGAAGGACAT CTTCGGAGCC 
AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGGGA ACTTGCTTTG 
GTACAAACAA AAACAATTAA AAAGACGCTG AACCCAAAGT GGAATGAAGA ATTTTATTTT 
AGAGTAAATC CATCTAATCA CAGACTCCTG TTTGAAGTAT TTGATGAAAA CAGACTGACA 
CGGGACGACT TCCTGGGACA GGTGGACATA CCCCTTAGTC ATCTCCCGAC AGAAGACCCA 
ACCATGGAGC GACCCTACAC ATTTAAGGAC TTTCTCCTCC GGCCAAGAAG TCATAAATCT 
CGGGTTAAGG GATTTTTGCG ATTGAAAATG GCCTACATGC CCAAAAACGG AGGCCAAGAT 
GAAGAGAACA GTGAGCAGCG GGATGAGATG GAGCCCGGGT GGGAAGTCGT TGACTCCAAT 
GACTCGGCTT CTCAGCACCA GGAGGAGCTG CCCCCGCCTC CGCTGCCCCC CGGGTGGGAA 
GAAAAGGTGG ACAACCTGGG CCGCACGTAC TACGTGAACC ACAACAACCG GACCACCCAG 
TGGCACCGGC CCAGCCTCAT GGACGTGTCG TCCGAGTCCG ACAGCAACAT CCGGCAGATC 
AACCAGGAGG CCGCGCATCG CCGCTTCCGC TCGCGCAGAC ATATCAGCGA GGACCTGGAG 
CCGGAGCCCA GCGAGGGAGG GGAGGGCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG 
AACATGAGCG GAGACTCGCT CAGCCTGGCG CTGCCCCCGC CACCAGCCTC GCCCGTGTCC 
CGGACGAGCC CCCAGGAGCT GTCCGAGGAG CTGAGCAGAA GGCTGCAGGT CACTCCCGAC 
TCCAACGGCG AGCAGTTTGG GTCTTTGATT CAGCGAGAGC CGTCCTCCCG GCTGAGGTCC 
TGCAGTGTCA CTGATGCAGT TGCGGAGCAG GCTCATCTCC CGCCGCCATC AGTGGCCTAT 
GTACACACCA CGCCGGGCCT GCCCTCGGGC TGGGAAGAGA GGAAAGATGC TAAGGGACGC 
ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGGCCTAT CGTGCAGCTC 
GCAGAAGACG GCGCGTCCGG ATCCGCCACA AACAGTAACA ACCACCTAAT CGAGCCTCAG 
ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCCGCCCC GCTGGAGGGC 
GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGATACCC TTTCCAATCC ACAGTCCCCA 
CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCCTGCCG 
CCCGGCTGGG AGATGAGGAT AGCGCCCAAC GGCCGGCCCT TCTTCATTGA CCACAACACA 
AAGACCACGA CGTGGGAAGA TCCACGCCTG AAGTTTCCAG TACACATGCG GTCAAAGGCA 
TCCTTAAACC CCAATGACCT TGGCCCTCTT CCTCCTGGCT GGGAAGAAAG GATCCACTTG 
GATGGCCGGA CGTTTTACAT TGACCACAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA 
CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTTCCTTACT CCAGAGAATT TAAGCAGAAA 
TATGACTACT TTAGAAAGAA ATTAAAGAAA CCTGCGGATA TTCCAAATAG GTTTGAAATG 
AAACTTCACA GAAATAACAT ATTTGAAGAA TCCTATCGGA GAATCATGTC TGTGAAAAGA 
CCAGATGTCC TGAAAGCTAG GCTGTGGATT GAGTTTGAAT CGGAGAAAGG TCTGGACTAC 
GGGGGCGTGG CCAGAGAGTG GTTCTTCCTA CTGTCCAAGG AGATGTTCAA CCCTTACTAC 
GGCCTCTTCG AGTACTCGGC AACGGACAAC TACACACTTC AGATCAACCC CAATTCAGGC 
CTCTGTAATG AGGACCATCT GTCCTATTTC ACCTTCATTG GAAGAGTTGC CGGGCTGGCA 
GTATTTCATG GGAAACTTCT AGATGGTTTC TTCATTCGAC CGTTTTATAA AATGATGCTG 
GGAAAGCAGA TAACTCTGAA TGACATGGAA TCTGTGGATA GCGAATATTA CAACTCTTTG 
AAATGGATCT TAGAAAATGA CCCAACTGAA CTGGACCTTA TGTTCTGCAT TGATGAAGAG 
AACTTTGGGC AGACCTATCA AGTGGATCTG AAGCCCAACG GGTCAGAAAT AATGGTAACA 
AATGAAAACA AGAGAGAGTA CATCGACCTC GTTATCCAGT GGAGGTTCGT GAACAGAGTC 
CAGAAGCAGA TGAATGCCTT CCTGGAGGGA TTCACGGAAC TGCTCCCTAT TGATTTGATT 
AAAATCTTTG ATGAAAATGA ACTGGAGTTG CTGATGTGCG GCCTTGGCGA TGTGGATGTG 
AACGACTGGC GACAACATTC TATTTACAAG AATGGCTACT GCCCGAACCA CCCCGTGATC 
CAGTGGTTCT GGAAGGCTGT GCTGCTGATG GACGCCGAGA GGCGCATCCG GTTACTGCAG 
TTTGTCACAG GGACGTCCCG AGTACCTATG AATGGATTTG CCGAGCTTTA TGGTTCCAAT 
GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC 
ACGTGCTTTA ATCGCCTTGA CTTACCTCCG TATGAAACCT TCGAAGATTT ACGAGAGAAG 
CTCCTCATGG CTGTGGAAAA CGCCCAAGGC TTCGAGGGGG TGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128954.1
CDS	187..3054
Translation	MATGLGEPVYGLSEEEGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_025273169.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025273169.1

ORF Insert Sequence:

ATGGCGACCG GGCTCGGGGA GCCGGTCTAC GGACTTTCGG AAGAAGAGGG AGAGTCCCGT 
ATTCTCAGAG TGAAGGTTGT TTCTGGAATT GATCTCGCCA AGAAGGACAT CTTCGGAGCC 
AGTGATCCGT ATGTGAAACT TTCATTGTAC GTAGCGGATG AGAATAGGGA ACTTGCTTTG 
GTACAAACAA AAACAATTAA AAAGACGCTG AACCCAAAGT GGAATGAAGA ATTTTATTTT 
AGAGTAAATC CATCTAATCA CAGACTCCTG TTTGAAGTAT TTGATGAAAA CAGACTGACA 
CGGGACGACT TCCTGGGACA GGTGGACATA CCCCTTAGTC ATCTCCCGAC AGAAGACCCA 
ACCATGGAGC GACCCTACAC ATTTAAGGAC TTTCTCCTCC GGCCAAGAAG TCATAAATCT 
CGGGTTAAGG GATTTTTGCG ATTGAAAATG GCCTACATGC CCAAAAACGG AGGCCAAGAT 
GAAGAGAACA GTGAGCAGCG GGATGAGATG GAGCCCGGGT GGGAAGTCGT TGACTCCAAT 
GACTCGGCTT CTCAGCACCA GGAGGAGCTG CCCCCGCCTC CGCTGCCCCC CGGGTGGGAA 
GAAAAGGTGG ACAACCTGGG CCGCACGTAC TACGTGAACC ACAACAACCG GACCACCCAG 
TGGCACCGGC CCAGCCTCAT GGACGTGTCG TCCGAGTCCG ACAGCAACAT CCGGCAGATC 
AACCAGGAGG CCGCGCATCG CCGCTTCCGC TCGCGCAGAC ATATCAGCGA GGACCTGGAG 
CCGGAGCCCA GCGAGGGAGG GGAGGGCCCC GAGCCTTGGG AGACCATTTC AGAGGAAGTG 
AACATGAGCG GAGACTCGCT CAGCCTGGCG CTGCCCCCGC CACCAGCCTC GCCCGTGTCC 
CGGACGAGCC CCCAGGAGCT GTCCGAGGAG CTGAGCAGAA GGCTGCAGGT CACTCCCGAC 
TCCAACGGCG AGCAGTTTGG GTCTTTGATT CAGCGAGAGC CGTCCTCCCG GCTGAGGTCC 
TGCAGTGTCA CTGATGCAGT TGCGGAGCAG GCTCATCTCC CGCCGCCATC AGTGGCCTAT 
GTACACACCA CGCCGGGCCT GCCCTCGGGC TGGGAAGAGA GGAAAGATGC TAAGGGACGC 
ACATACTATG TCAATCATAA CAATCGAACC ACAACTTGGA CTCGGCCTAT CGTGCAGCTC 
GCAGAAGACG GCGCGTCCGG ATCCGCCACA AACAGTAACA ACCACCTAAT CGAGCCTCAG 
ATCCGCCGGC CTCGTAGCCT CAGCTCGCCA ACAGTAACTT TATCCGCCCC GCTGGAGGGC 
GCCAAGGACT CACCCGTACG TCGGGCTGTG AAAGATACCC TTTCCAATCC ACAGTCCCCA 
CAGCCATCAC CTTACAACTC CCCCAAACCA CAACACAAAG TCACACAGAG CTTCCTGCCG 
CCCGGCTGGG AGATGAGGAT AGCGCCCAAC GGCCGGCCCT TCTTCATTGA CCACAACACA 
AAGACCACGA CGTGGGAAGA TCCACGCCTG AAGTTTCCAG TACACATGCG GTCAAAGGCA 
TCCTTAAACC CCAATGACCT TGGCCCTCTT CCTCCTGGCT GGGAAGAAAG GATCCACTTG 
GATGGCCGGA CGTTTTACAT TGACCACAAT AGCAAAATTA CTCAGTGGGA AGACCCAAGA 
CTGCAGAACC CAGCTATTAC TGGTCCGGCT GTTCCTTACT CCAGAGAATT TAAGCAGAAA 
TATGACTACT TTAGAAAGAA ATTAAAGAAA CCTGCGGATA TTCCAAATAG GTTTGAAATG 
AAACTTCACA GAAATAACAT ATTTGAAGAA TCCTATCGGA GAATCATGTC TGTGAAAAGA 
CCAGATGTCC TGAAAGCTAG GCTGTGGATT GAGTTTGAAT CGGAGAAAGG TCTGGACTAC 
GGGGGCGTGG CCAGAGAGTG GTTCTTCCTA CTGTCCAAGG AGATGTTCAA CCCTTACTAC 
GGCCTCTTCG AGTACTCGGC AACGGACAAC TACACACTTC AGATCAACCC CAATTCAGGC 
CTCTGTAATG AGGACCATCT GTCCTATTTC ACCTTCATTG GAAGAGTTGC CGGGCTGGCA 
GTATTTCATG GGAAACTTCT AGATGGTTTC TTCATTCGAC CGTTTTATAA AATGATGCTG 
GGAAAGCAGA TAACTCTGAA TGACATGGAA TCTGTGGATA GCGAATATTA CAACTCTTTG 
AAATGGATCT TAGAAAATGA CCCAACTGAA CTGGACCTTA TGTTCTGCAT TGATGAAGAG 
AACTTTGGGC AGACCTATCA AGTGGATCTG AAGCCCAACG GGTCAGAAAT AATGGTAACA 
AATGAAAACA AGAGAGAGTA CATCGACCTC GTTATCCAGT GGAGGTTCGT GAACAGAGTC 
CAGAAGCAGA TGAATGCCTT CCTGGAGGGA TTCACGGAAC TGCTCCCTAT TGATTTGATT 
AAAATCTTTG ATGAAAATGA ACTGGAGTTG CTGATGTGCG GCCTTGGCGA TGTGGATGTG 
AACGACTGGC GACAACATTC TATTTACAAG AATGGCTACT GCCCGAACCA CCCCGTGATC 
CAGTGGTTCT GGAAGGCTGT GCTGCTGATG GACGCCGAGA GGCGCATCCG GTTACTGCAG 
TTTGTCACAG GGACGTCCCG AGTACCTATG AATGGATTTG CCGAGCTTTA TGGTTCCAAT 
GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAAGTCCTG AAAAACTGCC CAGAGCTCAC 
ACGTGCTTTA ATCGCCTTGA CTTACCTCCG TATGAAACCT TCGAAGATTT ACGAGAGAAG 
CTCCTCATGG CTGTGGAAAA CGCCCAAGGC TTCGAGGGGG TGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073372.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073434.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBu58360
Clone ID Related Accession (Same CDS sequence)	XM_006073372.2 , XM_006073373.2
Accession Version	XM_006073372.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006073372.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
GTTAAGGGAT TTTTGCGATT GAAAATGGCC TACATGCCCA AAAACGGAGG CCAAGATGAA 
GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC 
TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAA 
AAGGTGGACA ACCTGGGCCG CACGTACTAC GTGAACCACA ACAACCGGAC CACCCAGTGG 
CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC 
CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG 
GAGCCCAGCG AGGGAGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC 
ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CAGCCTCGCC CGTGTCCCGG 
ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC 
AACGGCGAGC AGTTTGGGTC TTTGATTCAG CGAGAGCCGT CCTCCCGGCT GAGGTCCTGC 
AGTGTCACTG ATGCAGTTGC GGAGCAGGCT CATCTCCCGC CGCCCAGTGC CCCAGCTGGG 
AGAGCACGTT CATCAACTGT CACCGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA 
CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA 
TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCGT GCAGCTCGCA 
GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC 
CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC 
AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG 
CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC 
GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG 
ACCACGACGT GGGAAGATCC ACGCCTGAAG TTTCCAGTAC ACATGCGGTC AAAGGCATCC 
TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT 
GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA 
GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC 
CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC 
TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC 
AGAAATAACA TATTTGAAGA ATCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC 
CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG 
GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC 
GAGTACTCGG CAACGGACAA CTACACACTT CAGATCAACC CCAATTCAGG CCTCTGTAAT 
GAGGACCATC TGTCCTATTT CACCTTCATT GGAAGAGTTG CCGGGCTGGC AGTATTTCAT 
GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG 
ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC 
TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG 
CAGACCTATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC AAATGAAAAC 
AAGAGAGAGT ACATCGACCT CGTTATCCAG TGGAGGTTCG TGAACAGAGT CCAGAAGCAG 
ATGAATGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT 
GATGAAAATG AACTGGAGTT GCTGATGTGC GGCCTTGGCG ATGTGGATGT GAACGACTGG 
CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC 
TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AGGCGCATCC GGTTACTGCA GTTTGTCACA 
GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG 
CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT 
AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG 
GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073434.1
CDS	374..2992
Translation	MERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSAPAGRARSSTVTGGEAPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHSSQVLCGEDDARDSVPSYDSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073372.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073372.2

ORF Insert Sequence:

ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
GTTAAGGGAT TTTTGCGATT GAAAATGGCC TACATGCCCA AAAACGGAGG CCAAGATGAA 
GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC 
TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAA 
AAGGTGGACA ACCTGGGCCG CACGTACTAC GTGAACCACA ACAACCGGAC CACCCAGTGG 
CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC 
CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG 
GAGCCCAGCG AGGGAGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC 
ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CAGCCTCGCC CGTGTCCCGG 
ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC 
AACGGCGAGC AGTTTGGGTC TTTGATTCAG CGAGAGCCGT CCTCCCGGCT GAGGTCCTGC 
AGTGTCACTG ATGCAGTTGC GGAGCAGGCT CATCTCCCGC CGCCCAGTGC CCCAGCTGGG 
AGAGCACGTT CATCAACTGT CACCGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA 
CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA 
TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCGT GCAGCTCGCA 
GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC 
CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC 
AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG 
CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC 
GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG 
ACCACGACGT GGGAAGATCC ACGCCTGAAG TTTCCAGTAC ACATGCGGTC AAAGGCATCC 
TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT 
GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA 
GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC 
CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC 
TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC 
AGAAATAACA TATTTGAAGA ATCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC 
CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG 
GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC 
GAGTACTCGG CAACGGACAA CTACACACTT CAGATCAACC CCAATTCAGG CCTCTGTAAT 
GAGGACCATC TGTCCTATTT CACCTTCATT GGAAGAGTTG CCGGGCTGGC AGTATTTCAT 
GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG 
ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC 
TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG 
CAGACCTATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC AAATGAAAAC 
AAGAGAGAGT ACATCGACCT CGTTATCCAG TGGAGGTTCG TGAACAGAGT CCAGAAGCAG 
ATGAATGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT 
GATGAAAATG AACTGGAGTT GCTGATGTGC GGCCTTGGCG ATGTGGATGT GAACGACTGG 
CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC 
TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AGGCGCATCC GGTTACTGCA GTTTGTCACA 
GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG 
CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT 
AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG 
GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073373.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073435.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBu58360
Clone ID Related Accession (Same CDS sequence)	XM_006073372.2 , XM_006073373.2
Accession Version	XM_006073373.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006073373.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
GTTAAGGGAT TTTTGCGATT GAAAATGGCC TACATGCCCA AAAACGGAGG CCAAGATGAA 
GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC 
TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAA 
AAGGTGGACA ACCTGGGCCG CACGTACTAC GTGAACCACA ACAACCGGAC CACCCAGTGG 
CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC 
CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG 
GAGCCCAGCG AGGGAGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC 
ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CAGCCTCGCC CGTGTCCCGG 
ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC 
AACGGCGAGC AGTTTGGGTC TTTGATTCAG CGAGAGCCGT CCTCCCGGCT GAGGTCCTGC 
AGTGTCACTG ATGCAGTTGC GGAGCAGGCT CATCTCCCGC CGCCCAGTGC CCCAGCTGGG 
AGAGCACGTT CATCAACTGT CACCGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA 
CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA 
TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCGT GCAGCTCGCA 
GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC 
CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC 
AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG 
CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC 
GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG 
ACCACGACGT GGGAAGATCC ACGCCTGAAG TTTCCAGTAC ACATGCGGTC AAAGGCATCC 
TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT 
GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA 
GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC 
CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC 
TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC 
AGAAATAACA TATTTGAAGA ATCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC 
CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG 
GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC 
GAGTACTCGG CAACGGACAA CTACACACTT CAGATCAACC CCAATTCAGG CCTCTGTAAT 
GAGGACCATC TGTCCTATTT CACCTTCATT GGAAGAGTTG CCGGGCTGGC AGTATTTCAT 
GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG 
ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC 
TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG 
CAGACCTATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC AAATGAAAAC 
AAGAGAGAGT ACATCGACCT CGTTATCCAG TGGAGGTTCG TGAACAGAGT CCAGAAGCAG 
ATGAATGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT 
GATGAAAATG AACTGGAGTT GCTGATGTGC GGCCTTGGCG ATGTGGATGT GAACGACTGG 
CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC 
TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AGGCGCATCC GGTTACTGCA GTTTGTCACA 
GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG 
CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT 
AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG 
GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073435.1
CDS	303..2921
Translation	MERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSAPAGRARSSTVTGGEAPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHSSQVLCGEDDARDSVPSYDSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073373.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073373.2

ORF Insert Sequence:

ATGGAGCGAC CCTACACATT TAAGGACTTT CTCCTCCGGC CAAGAAGTCA TAAATCTCGG 
GTTAAGGGAT TTTTGCGATT GAAAATGGCC TACATGCCCA AAAACGGAGG CCAAGATGAA 
GAGAACAGTG AGCAGCGGGA TGAGATGGAG CCCGGGTGGG AAGTCGTTGA CTCCAATGAC 
TCGGCTTCTC AGCACCAGGA GGAGCTGCCC CCGCCTCCGC TGCCCCCCGG GTGGGAAGAA 
AAGGTGGACA ACCTGGGCCG CACGTACTAC GTGAACCACA ACAACCGGAC CACCCAGTGG 
CACCGGCCCA GCCTCATGGA CGTGTCGTCC GAGTCCGACA GCAACATCCG GCAGATCAAC 
CAGGAGGCCG CGCATCGCCG CTTCCGCTCG CGCAGACATA TCAGCGAGGA CCTGGAGCCG 
GAGCCCAGCG AGGGAGGGGA GGGCCCCGAG CCTTGGGAGA CCATTTCAGA GGAAGTGAAC 
ATGAGCGGAG ACTCGCTCAG CCTGGCGCTG CCCCCGCCAC CAGCCTCGCC CGTGTCCCGG 
ACGAGCCCCC AGGAGCTGTC CGAGGAGCTG AGCAGAAGGC TGCAGGTCAC TCCCGACTCC 
AACGGCGAGC AGTTTGGGTC TTTGATTCAG CGAGAGCCGT CCTCCCGGCT GAGGTCCTGC 
AGTGTCACTG ATGCAGTTGC GGAGCAGGCT CATCTCCCGC CGCCCAGTGC CCCAGCTGGG 
AGAGCACGTT CATCAACTGT CACCGGTGGT GAGGCGCCAA CGCCATCAGT GGCCTATGTA 
CACACCACGC CGGGCCTGCC CTCGGGCTGG GAAGAGAGGA AAGATGCTAA GGGACGCACA 
TACTATGTCA ATCATAACAA TCGAACCACA ACTTGGACTC GGCCTATCGT GCAGCTCGCA 
GAAGACGGCG CGTCCGGATC CGCCACAAAC AGTAACAACC ACCTAATCGA GCCTCAGATC 
CGCCGGCCTC GTAGCCTCAG CTCGCCAACA GTAACTTTAT CCGCCCCGCT GGAGGGCGCC 
AAGGACTCAC CCGTACGTCG GGCTGTGAAA GATACCCTTT CCAATCCACA GTCCCCACAG 
CCATCACCTT ACAACTCCCC CAAACCACAA CACAAAGTCA CACAGAGCTT CCTGCCGCCC 
GGCTGGGAGA TGAGGATAGC GCCCAACGGC CGGCCCTTCT TCATTGACCA CAACACAAAG 
ACCACGACGT GGGAAGATCC ACGCCTGAAG TTTCCAGTAC ACATGCGGTC AAAGGCATCC 
TTAAACCCCA ATGACCTTGG CCCTCTTCCT CCTGGCTGGG AAGAAAGGAT CCACTTGGAT 
GGCCGGACGT TTTACATTGA CCACAGCAGC CAGGTGTTAT GTGGCGAGGA CGATGCCAGA 
GACTCAGTGC CCTCGTACGA TAGCAAAATT ACTCAGTGGG AAGACCCAAG ACTGCAGAAC 
CCAGCTATTA CTGGTCCGGC TGTTCCTTAC TCCAGAGAAT TTAAGCAGAA ATATGACTAC 
TTTAGAAAGA AATTAAAGAA ACCTGCGGAT ATTCCAAATA GGTTTGAAAT GAAACTTCAC 
AGAAATAACA TATTTGAAGA ATCCTATCGG AGAATCATGT CTGTGAAAAG ACCAGATGTC 
CTGAAAGCTA GGCTGTGGAT TGAGTTTGAA TCGGAGAAAG GTCTGGACTA CGGGGGCGTG 
GCCAGAGAGT GGTTCTTCCT ACTGTCCAAG GAGATGTTCA ACCCTTACTA CGGCCTCTTC 
GAGTACTCGG CAACGGACAA CTACACACTT CAGATCAACC CCAATTCAGG CCTCTGTAAT 
GAGGACCATC TGTCCTATTT CACCTTCATT GGAAGAGTTG CCGGGCTGGC AGTATTTCAT 
GGGAAACTTC TAGATGGTTT CTTCATTCGA CCGTTTTATA AAATGATGCT GGGAAAGCAG 
ATAACTCTGA ATGACATGGA ATCTGTGGAT AGCGAATATT ACAACTCTTT GAAATGGATC 
TTAGAAAATG ACCCAACTGA ACTGGACCTT ATGTTCTGCA TTGATGAAGA GAACTTTGGG 
CAGACCTATC AAGTGGATCT GAAGCCCAAC GGGTCAGAAA TAATGGTAAC AAATGAAAAC 
AAGAGAGAGT ACATCGACCT CGTTATCCAG TGGAGGTTCG TGAACAGAGT CCAGAAGCAG 
ATGAATGCCT TCCTGGAGGG ATTCACGGAA CTGCTCCCTA TTGATTTGAT TAAAATCTTT 
GATGAAAATG AACTGGAGTT GCTGATGTGC GGCCTTGGCG ATGTGGATGT GAACGACTGG 
CGACAACATT CTATTTACAA GAATGGCTAC TGCCCGAACC ACCCCGTGAT CCAGTGGTTC 
TGGAAGGCTG TGCTGCTGAT GGACGCCGAG AGGCGCATCC GGTTACTGCA GTTTGTCACA 
GGGACGTCCC GAGTACCTAT GAATGGATTT GCCGAGCTTT ATGGTTCCAA TGGTCCTCAG 
CTGTTTACAA TAGAGCAATG GGGAAGTCCT GAAAAACTGC CCAGAGCTCA CACGTGCTTT 
AATCGCCTTG ACTTACCTCC GTATGAAACC TTCGAAGATT TACGAGAGAA GCTCCTCATG 
GCTGTGGAAA ACGCCCAAGG CTTCGAGGGG GTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073363.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073425.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.

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CloneID	OBu58349
Clone ID Related Accession (Same CDS sequence)	XM_006073363.2
Accession Version	XM_006073363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006073363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGAGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGCC CAGTGCCCCA 
GCTGGGAGAG CACGTTCATC AACTGTCACC GGTGGTGAGG CGCCAACGCC ATCAGTGGCC 
TATGTACACA CCACGCCGGG CCTGCCCTCG GGCTGGGAAG AGAGGAAAGA TGCTAAGGGA 
CGCACATACT ATGTCAATCA TAACAATCGA ACCACAACTT GGACTCGGCC TATCGTGCAG 
CTCGCAGAAG ACGGCGCGTC CGGATCCGCC ACAAACAGTA ACAACCACCT AATCGAGCCT 
CAGATCCGCC GGCCTCGTAG CCTCAGCTCG CCAACAGTAA CTTTATCCGC CCCGCTGGAG 
GGCGCCAAGG ACTCACCCGT ACGTCGGGCT GTGAAAGATA CCCTTTCCAA TCCACAGTCC 
CCACAGCCAT CACCTTACAA CTCCCCCAAA CCACAACACA AAGTCACACA GAGCTTCCTG 
CCGCCCGGCT GGGAGATGAG GATAGCGCCC AACGGCCGGC CCTTCTTCAT TGACCACAAC 
ACAAAGACCA CGACGTGGGA AGATCCACGC CTGAAGTTTC CAGTACACAT GCGGTCAAAG 
GCATCCTTAA ACCCCAATGA CCTTGGCCCT CTTCCTCCTG GCTGGGAAGA AAGGATCCAC 
TTGGATGGCC GGACGTTTTA CATTGACCAC AGCAGCCAGG TGTTATGTGG CGAGGACGAT 
GCCAGAGACT CAGTGCCCTC GTACGATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG 
CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT 
GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA 
CTTCACAGAA ATAACATATT TGAAGAATCC TATCGGAGAA TCATGTCTGT GAAAAGACCA 
GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG 
GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC 
CTCTTCGAGT ACTCGGCAAC GGACAACTAC ACACTTCAGA TCAACCCCAA TTCAGGCCTC 
TGTAATGAGG ACCATCTGTC CTATTTCACC TTCATTGGAA GAGTTGCCGG GCTGGCAGTA 
TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA 
AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA 
TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC 
TTTGGGCAGA CCTATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACAAAT 
GAAAACAAGA GAGAGTACAT CGACCTCGTT ATCCAGTGGA GGTTCGTGAA CAGAGTCCAG 
AAGCAGATGA ATGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA 
ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGCGGCC TTGGCGATGT GGATGTGAAC 
GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG 
TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAGGC GCATCCGGTT ACTGCAGTTT 
GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT 
CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG 
TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC 
CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073425.1
CDS	86..3310
Translation	MPSGAEGQAGPAAAAPEPRRWSLQHVPDASGSSGKRCFVFQLQQPPASGGGPAPSGDFNFGFTGTKGDRLVRYPRIRLERSSSYPTQPRAELGSPPEERGHPDPEAPLRGRRVAPDAHRTEAVAERSPQGQGCTFKIRQDQNAGQQHFRILVTRGPEEAPQSPEEDAAAAGPASTGAGAPTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSAPAGRARSSTVTGGEAPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHSSQVLCGEDDARDSVPSYDSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073363.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073363.2

ORF Insert Sequence:

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGAGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGCC CAGTGCCCCA 
GCTGGGAGAG CACGTTCATC AACTGTCACC GGTGGTGAGG CGCCAACGCC ATCAGTGGCC 
TATGTACACA CCACGCCGGG CCTGCCCTCG GGCTGGGAAG AGAGGAAAGA TGCTAAGGGA 
CGCACATACT ATGTCAATCA TAACAATCGA ACCACAACTT GGACTCGGCC TATCGTGCAG 
CTCGCAGAAG ACGGCGCGTC CGGATCCGCC ACAAACAGTA ACAACCACCT AATCGAGCCT 
CAGATCCGCC GGCCTCGTAG CCTCAGCTCG CCAACAGTAA CTTTATCCGC CCCGCTGGAG 
GGCGCCAAGG ACTCACCCGT ACGTCGGGCT GTGAAAGATA CCCTTTCCAA TCCACAGTCC 
CCACAGCCAT CACCTTACAA CTCCCCCAAA CCACAACACA AAGTCACACA GAGCTTCCTG 
CCGCCCGGCT GGGAGATGAG GATAGCGCCC AACGGCCGGC CCTTCTTCAT TGACCACAAC 
ACAAAGACCA CGACGTGGGA AGATCCACGC CTGAAGTTTC CAGTACACAT GCGGTCAAAG 
GCATCCTTAA ACCCCAATGA CCTTGGCCCT CTTCCTCCTG GCTGGGAAGA AAGGATCCAC 
TTGGATGGCC GGACGTTTTA CATTGACCAC AGCAGCCAGG TGTTATGTGG CGAGGACGAT 
GCCAGAGACT CAGTGCCCTC GTACGATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG 
CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT 
GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA 
CTTCACAGAA ATAACATATT TGAAGAATCC TATCGGAGAA TCATGTCTGT GAAAAGACCA 
GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG 
GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC 
CTCTTCGAGT ACTCGGCAAC GGACAACTAC ACACTTCAGA TCAACCCCAA TTCAGGCCTC 
TGTAATGAGG ACCATCTGTC CTATTTCACC TTCATTGGAA GAGTTGCCGG GCTGGCAGTA 
TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA 
AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA 
TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC 
TTTGGGCAGA CCTATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACAAAT 
GAAAACAAGA GAGAGTACAT CGACCTCGTT ATCCAGTGGA GGTTCGTGAA CAGAGTCCAG 
AAGCAGATGA ATGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA 
ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGCGGCC TTGGCGATGT GGATGTGAAC 
GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG 
TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAGGC GCATCCGGTT ACTGCAGTTT 
GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT 
CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG 
TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC 
CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073364.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073426.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu58350
Clone ID Related Accession (Same CDS sequence)	XM_006073364.2
Accession Version	XM_006073364.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006073364.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGAGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGCC CAGTGCCCCA 
GCTGGGAGAG CACGTTCATC AACTGTCACC GGTGGTGAGG CGCCAACGCC ATCAGTGGCC 
TATGTACACA CCACGCCGGG CCTGCCCTCG GGCTGGGAAG AGAGGAAAGA TGCTAAGGGA 
CGCACATACT ATGTCAATCA TAACAATCGA ACCACAACTT GGACTCGGCC TATCGTGCAG 
CTCGCAGAAG ACGGCGCGTC CGGATCCGCC ACAAACAGTA ACAACCACCT AATCGAGCCT 
CAGATCCGCC GGCCTCGTAG CCTCAGCTCG CCAACAGTAA CTTTATCCGC CCCGCTGGAG 
GGCGCCAAGG ACTCACCCGT ACGTCGGGCT GTGAAAGATA CCCTTTCCAA TCCACAGTCC 
CCACAGCCAT CACCTTACAA CTCCCCCAAA CCACAACACA AAGTCACACA GAGCTTCCTG 
CCGCCCGGCT GGGAGATGAG GATAGCGCCC AACGGCCGGC CCTTCTTCAT TGACCACAAC 
ACAAAGACCA CGACGTGGGA AGATCCACGC CTGAAGTTTC CAGTACACAT GCGGTCAAAG 
GCATCCTTAA ACCCCAATGA CCTTGGCCCT CTTCCTCCTG GCTGGGAAGA AAGGATCCAC 
TTGGATGGCC GGACGTTTTA CATTGACCAC AATAGCAAAA TTACTCAGTG GGAAGACCCA 
AGACTGCAGA ACCCAGCTAT TACTGGTCCG GCTGTTCCTT ACTCCAGAGA ATTTAAGCAG 
AAATATGACT ACTTTAGAAA GAAATTAAAG AAACCTGCGG ATATTCCAAA TAGGTTTGAA 
ATGAAACTTC ACAGAAATAA CATATTTGAA GAATCCTATC GGAGAATCAT GTCTGTGAAA 
AGACCAGATG TCCTGAAAGC TAGGCTGTGG ATTGAGTTTG AATCGGAGAA AGGTCTGGAC 
TACGGGGGCG TGGCCAGAGA GTGGTTCTTC CTACTGTCCA AGGAGATGTT CAACCCTTAC 
TACGGCCTCT TCGAGTACTC GGCAACGGAC AACTACACAC TTCAGATCAA CCCCAATTCA 
GGCCTCTGTA ATGAGGACCA TCTGTCCTAT TTCACCTTCA TTGGAAGAGT TGCCGGGCTG 
GCAGTATTTC ATGGGAAACT TCTAGATGGT TTCTTCATTC GACCGTTTTA TAAAATGATG 
CTGGGAAAGC AGATAACTCT GAATGACATG GAATCTGTGG ATAGCGAATA TTACAACTCT 
TTGAAATGGA TCTTAGAAAA TGACCCAACT GAACTGGACC TTATGTTCTG CATTGATGAA 
GAGAACTTTG GGCAGACCTA TCAAGTGGAT CTGAAGCCCA ACGGGTCAGA AATAATGGTA 
ACAAATGAAA ACAAGAGAGA GTACATCGAC CTCGTTATCC AGTGGAGGTT CGTGAACAGA 
GTCCAGAAGC AGATGAATGC CTTCCTGGAG GGATTCACGG AACTGCTCCC TATTGATTTG 
ATTAAAATCT TTGATGAAAA TGAACTGGAG TTGCTGATGT GCGGCCTTGG CGATGTGGAT 
GTGAACGACT GGCGACAACA TTCTATTTAC AAGAATGGCT ACTGCCCGAA CCACCCCGTG 
ATCCAGTGGT TCTGGAAGGC TGTGCTGCTG ATGGACGCCG AGAGGCGCAT CCGGTTACTG 
CAGTTTGTCA CAGGGACGTC CCGAGTACCT ATGAATGGAT TTGCCGAGCT TTATGGTTCC 
AATGGTCCTC AGCTGTTTAC AATAGAGCAA TGGGGAAGTC CTGAAAAACT GCCCAGAGCT 
CACACGTGCT TTAATCGCCT TGACTTACCT CCGTATGAAA CCTTCGAAGA TTTACGAGAG 
AAGCTCCTCA TGGCTGTGGA AAACGCCCAA GGCTTCGAGG GGGTGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073426.1
CDS	79..3249
Translation	MPSGAEGQAGPAAAAPEPRRWSLQHVPDASGSSGKRCFVFQLQQPPASGGGPAPSGDFNFGFTGTKGDRLVRYPRIRLERSSSYPTQPRAELGSPPEERGHPDPEAPLRGRRVAPDAHRTEAVAERSPQGQGCTFKIRQDQNAGQQHFRILVTRGPEEAPQSPEEDAAAAGPASTGAGAPTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSAPAGRARSSTVTGGEAPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073364.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073364.2

ORF Insert Sequence:

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGAGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGCC CAGTGCCCCA 
GCTGGGAGAG CACGTTCATC AACTGTCACC GGTGGTGAGG CGCCAACGCC ATCAGTGGCC 
TATGTACACA CCACGCCGGG CCTGCCCTCG GGCTGGGAAG AGAGGAAAGA TGCTAAGGGA 
CGCACATACT ATGTCAATCA TAACAATCGA ACCACAACTT GGACTCGGCC TATCGTGCAG 
CTCGCAGAAG ACGGCGCGTC CGGATCCGCC ACAAACAGTA ACAACCACCT AATCGAGCCT 
CAGATCCGCC GGCCTCGTAG CCTCAGCTCG CCAACAGTAA CTTTATCCGC CCCGCTGGAG 
GGCGCCAAGG ACTCACCCGT ACGTCGGGCT GTGAAAGATA CCCTTTCCAA TCCACAGTCC 
CCACAGCCAT CACCTTACAA CTCCCCCAAA CCACAACACA AAGTCACACA GAGCTTCCTG 
CCGCCCGGCT GGGAGATGAG GATAGCGCCC AACGGCCGGC CCTTCTTCAT TGACCACAAC 
ACAAAGACCA CGACGTGGGA AGATCCACGC CTGAAGTTTC CAGTACACAT GCGGTCAAAG 
GCATCCTTAA ACCCCAATGA CCTTGGCCCT CTTCCTCCTG GCTGGGAAGA AAGGATCCAC 
TTGGATGGCC GGACGTTTTA CATTGACCAC AATAGCAAAA TTACTCAGTG GGAAGACCCA 
AGACTGCAGA ACCCAGCTAT TACTGGTCCG GCTGTTCCTT ACTCCAGAGA ATTTAAGCAG 
AAATATGACT ACTTTAGAAA GAAATTAAAG AAACCTGCGG ATATTCCAAA TAGGTTTGAA 
ATGAAACTTC ACAGAAATAA CATATTTGAA GAATCCTATC GGAGAATCAT GTCTGTGAAA 
AGACCAGATG TCCTGAAAGC TAGGCTGTGG ATTGAGTTTG AATCGGAGAA AGGTCTGGAC 
TACGGGGGCG TGGCCAGAGA GTGGTTCTTC CTACTGTCCA AGGAGATGTT CAACCCTTAC 
TACGGCCTCT TCGAGTACTC GGCAACGGAC AACTACACAC TTCAGATCAA CCCCAATTCA 
GGCCTCTGTA ATGAGGACCA TCTGTCCTAT TTCACCTTCA TTGGAAGAGT TGCCGGGCTG 
GCAGTATTTC ATGGGAAACT TCTAGATGGT TTCTTCATTC GACCGTTTTA TAAAATGATG 
CTGGGAAAGC AGATAACTCT GAATGACATG GAATCTGTGG ATAGCGAATA TTACAACTCT 
TTGAAATGGA TCTTAGAAAA TGACCCAACT GAACTGGACC TTATGTTCTG CATTGATGAA 
GAGAACTTTG GGCAGACCTA TCAAGTGGAT CTGAAGCCCA ACGGGTCAGA AATAATGGTA 
ACAAATGAAA ACAAGAGAGA GTACATCGAC CTCGTTATCC AGTGGAGGTT CGTGAACAGA 
GTCCAGAAGC AGATGAATGC CTTCCTGGAG GGATTCACGG AACTGCTCCC TATTGATTTG 
ATTAAAATCT TTGATGAAAA TGAACTGGAG TTGCTGATGT GCGGCCTTGG CGATGTGGAT 
GTGAACGACT GGCGACAACA TTCTATTTAC AAGAATGGCT ACTGCCCGAA CCACCCCGTG 
ATCCAGTGGT TCTGGAAGGC TGTGCTGCTG ATGGACGCCG AGAGGCGCAT CCGGTTACTG 
CAGTTTGTCA CAGGGACGTC CCGAGTACCT ATGAATGGAT TTGCCGAGCT TTATGGTTCC 
AATGGTCCTC AGCTGTTTAC AATAGAGCAA TGGGGAAGTC CTGAAAAACT GCCCAGAGCT 
CACACGTGCT TTAATCGCCT TGACTTACCT CCGTATGAAA CCTTCGAAGA TTTACGAGAG 
AAGCTCCTCA TGGCTGTGGA AAACGCCCAA GGCTTCGAGG GGGTGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073365.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073427.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu58351
Clone ID Related Accession (Same CDS sequence)	XM_006073365.2
Accession Version	XM_006073365.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006073365.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGAGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGCC ATCAGTGGCC 
TATGTACACA CCACGCCGGG CCTGCCCTCG GGCTGGGAAG AGAGGAAAGA TGCTAAGGGA 
CGCACATACT ATGTCAATCA TAACAATCGA ACCACAACTT GGACTCGGCC TATCGTGCAG 
CTCGCAGAAG ACGGCGCGTC CGGATCCGCC ACAAACAGTA ACAACCACCT AATCGAGCCT 
CAGATCCGCC GGCCTCGTAG CCTCAGCTCG CCAACAGTAA CTTTATCCGC CCCGCTGGAG 
GGCGCCAAGG ACTCACCCGT ACGTCGGGCT GTGAAAGATA CCCTTTCCAA TCCACAGTCC 
CCACAGCCAT CACCTTACAA CTCCCCCAAA CCACAACACA AAGTCACACA GAGCTTCCTG 
CCGCCCGGCT GGGAGATGAG GATAGCGCCC AACGGCCGGC CCTTCTTCAT TGACCACAAC 
ACAAAGACCA CGACGTGGGA AGATCCACGC CTGAAGTTTC CAGTACACAT GCGGTCAAAG 
GCATCCTTAA ACCCCAATGA CCTTGGCCCT CTTCCTCCTG GCTGGGAAGA AAGGATCCAC 
TTGGATGGCC GGACGTTTTA CATTGACCAC AGCAGCCAGG TGTTATGTGG CGAGGACGAT 
GCCAGAGACT CAGTGCCCTC GTACGATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG 
CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT 
GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA 
CTTCACAGAA ATAACATATT TGAAGAATCC TATCGGAGAA TCATGTCTGT GAAAAGACCA 
GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG 
GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC 
CTCTTCGAGT ACTCGGCAAC GGACAACTAC ACACTTCAGA TCAACCCCAA TTCAGGCCTC 
TGTAATGAGG ACCATCTGTC CTATTTCACC TTCATTGGAA GAGTTGCCGG GCTGGCAGTA 
TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA 
AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA 
TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC 
TTTGGGCAGA CCTATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACAAAT 
GAAAACAAGA GAGAGTACAT CGACCTCGTT ATCCAGTGGA GGTTCGTGAA CAGAGTCCAG 
AAGCAGATGA ATGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA 
ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGCGGCC TTGGCGATGT GGATGTGAAC 
GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG 
TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAGGC GCATCCGGTT ACTGCAGTTT 
GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT 
CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG 
TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC 
CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073427.1
CDS	83..3247
Translation	MPSGAEGQAGPAAAAPEPRRWSLQHVPDASGSSGKRCFVFQLQQPPASGGGPAPSGDFNFGFTGTKGDRLVRYPRIRLERSSSYPTQPRAELGSPPEERGHPDPEAPLRGRRVAPDAHRTEAVAERSPQGQGCTFKIRQDQNAGQQHFRILVTRGPEEAPQSPEEDAAAAGPASTGAGAPTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHSSQVLCGEDDARDSVPSYDSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073365.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073365.2

ORF Insert Sequence:

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGAGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGCC ATCAGTGGCC 
TATGTACACA CCACGCCGGG CCTGCCCTCG GGCTGGGAAG AGAGGAAAGA TGCTAAGGGA 
CGCACATACT ATGTCAATCA TAACAATCGA ACCACAACTT GGACTCGGCC TATCGTGCAG 
CTCGCAGAAG ACGGCGCGTC CGGATCCGCC ACAAACAGTA ACAACCACCT AATCGAGCCT 
CAGATCCGCC GGCCTCGTAG CCTCAGCTCG CCAACAGTAA CTTTATCCGC CCCGCTGGAG 
GGCGCCAAGG ACTCACCCGT ACGTCGGGCT GTGAAAGATA CCCTTTCCAA TCCACAGTCC 
CCACAGCCAT CACCTTACAA CTCCCCCAAA CCACAACACA AAGTCACACA GAGCTTCCTG 
CCGCCCGGCT GGGAGATGAG GATAGCGCCC AACGGCCGGC CCTTCTTCAT TGACCACAAC 
ACAAAGACCA CGACGTGGGA AGATCCACGC CTGAAGTTTC CAGTACACAT GCGGTCAAAG 
GCATCCTTAA ACCCCAATGA CCTTGGCCCT CTTCCTCCTG GCTGGGAAGA AAGGATCCAC 
TTGGATGGCC GGACGTTTTA CATTGACCAC AGCAGCCAGG TGTTATGTGG CGAGGACGAT 
GCCAGAGACT CAGTGCCCTC GTACGATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG 
CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT 
GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA 
CTTCACAGAA ATAACATATT TGAAGAATCC TATCGGAGAA TCATGTCTGT GAAAAGACCA 
GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG 
GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC 
CTCTTCGAGT ACTCGGCAAC GGACAACTAC ACACTTCAGA TCAACCCCAA TTCAGGCCTC 
TGTAATGAGG ACCATCTGTC CTATTTCACC TTCATTGGAA GAGTTGCCGG GCTGGCAGTA 
TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA 
AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA 
TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC 
TTTGGGCAGA CCTATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACAAAT 
GAAAACAAGA GAGAGTACAT CGACCTCGTT ATCCAGTGGA GGTTCGTGAA CAGAGTCCAG 
AAGCAGATGA ATGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA 
ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGCGGCC TTGGCGATGT GGATGTGAAC 
GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG 
TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAGGC GCATCCGGTT ACTGCAGTTT 
GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT 
CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG 
TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC 
CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025273165.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128950.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu58352
Clone ID Related Accession (Same CDS sequence)	XM_025273165.1
Accession Version	XM_025273165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCATC GGCTTCGGTT TCATTTTGGC TCCTGCCGCA GCAACACAGC CCCCGAGTCG 
GAGATCCTAG ACCAGGAGGG AGAAGACGAC TTTTTCATGG CATTCCACAC GCTCCCGCGG 
CGGGGCGGCG CGGTCGCGCT GGCCCCGAGC GGAGCGCAGG ACGCGGGCCT GCGGGGAGGC 
GTGGGCGCGC TCAAGCGCAG CACATCCATG TTCATCCCGC AGCTGCTGGG CCCCGTGGAC 
TCGCGGCCCA CGCGGAGCTC GTCCGTGCAG ATCTCGCTGC AGCGCAAGGC GGCTGACGGC 
GCCCCTGACG ACGGCGACGC GGGCACGGAG CCGCCGGGGG AGCCCCTGGA GGCCAAGAGC 
CTCGACGACG GCGGCGGCGG CGGCGATGGC TCCCCTACCG GGGAGCCCGG GGCTCCAGGC 
CCGCAGCTCA ACGGCACGTG TGGCCGCCGC CGAGCGCCGG GCCCCGACGG CCGCGAGGAG 
GCCGAGCTGC CGGCCGTGCG TGTCCAACAC CGCGCGTCTA GCGCCGATGT GCGCCCGGTG 
AAGTTGATGC CCTCGGGAGC CGAGGGCCAG GCTGGGCCCG CAGCGGCGGC GCCCGAGCCC 
AGGCGCTGGT CCCTGCAGCA TGTTCCAGAT GCTTCTGGAA GCTCCGGGAA GCGCTGCTTT 
GTCTTCCAGC TGCAGCAGCC GCCAGCCAGT GGTGGCGGGC CTGCGCCGAG CGGCGACTTC 
AACTTCGGCT TCACCGGCAC CAAGGGGGAC AGGTTGGTGA GGTATCCCCG AATCCGGCTG 
GAGAGGAGCA GCTCGTACCC CACGCAGCCC CGGGCTGAGC TCGGGAGTCC CCCGGAGGAG 
CGCGGCCACC CAGACCCGGA GGCGCCCCTG CGGGGCCGCA GGGTGGCTCC CGATGCCCAC 
AGGACCGAGG CGGTGGCCGA GAGGTCACCA CAGGGCCAGG GCTGCACGTT TAAGATCAGG 
CAGGATCAAA ACGCGGGGCA GCAGCACTTC AGAATCCTCG TGACTCGGGG GCCCGAGGAA 
GCCCCCCAGA GTCCCGAGGA GGACGCCGCA GCGGCCGGCC CGGCTTCCAC GGGAGCCGGC 
GCCCCGACAC GGGACGACTT CCTGGGACAG GTGGACATAC CCCTTAGTCA TCTCCCGACA 
GAAGACCCAA CCATGGAGCG ACCCTACACA TTTAAGGACT TTCTCCTCCG GCCAAGAAGT 
CATAAATCTC GGGTTAAGGG ATTTTTGCGA TTGAAAATGG CCTACATGCC CAAAAACGGA 
GGCCAAGATG AAGAGAACAG TGAGCAGCGG GATGAGATGG AGCCCGGGTG GGAAGTCGTT 
GACTCCAATG ACTCGGCTTC TCAGCACCAG GAGGAGCTGC CCCCGCCTCC GCTGCCCCCC 
GGGTGGGAAG AAAAGGTGGA CAACCTGGGC CGCACGTACT ACGTGAACCA CAACAACCGG 
ACCACCCAGT GGCACCGGCC CAGCCTCATG GACGTGTCGT CCGAGTCCGA CAGCAACATC 
CGGCAGATCA ACCAGGAGGC CGCGCATCGC CGCTTCCGCT CGCGCAGACA TATCAGCGAG 
GACCTGGAGC CGGAGCCCAG CGAGGGAGGG GAGGGCCCCG AGCCTTGGGA GACCATTTCA 
GAGGAAGTGA ACATGAGCGG AGACTCGCTC AGCCTGGCGC TGCCCCCGCC ACCAGCCTCG 
CCCGTGTCCC GGACGAGCCC CCAGGAGCTG TCCGAGGAGC TGAGCAGAAG GCTGCAGGTC 
ACTCCCGACT CCAACGGCGA GCAGTTTGGG TCTTTGATTC AGCGAGAGCC GTCCTCCCGG 
CTGAGGTCCT GCAGTGTCAC TGATGCAGTT GCGGAGCAGG CTCATCTCCC GCCGCCCAGT 
GCCCCAGCTG GGAGAGCACG TTCATCAACT GTCACCGGTG GTGAGGCGCC AACGCCATCA 
GTGGCCTATG TACACACCAC GCCGGGCCTG CCCTCGGGCT GGGAAGAGAG GAAAGATGCT 
AAGGGACGCA CATACTATGT CAATCATAAC AATCGAACCA CAACTTGGAC TCGGCCTATC 
GTGCAGCTCG CAGAAGACGG CGCGTCCGGA TCCGCCACAA ACAGTAACAA CCACCTAATC 
GAGCCTCAGA TCCGCCGGCC TCGTAGCCTC AGCTCGCCAA CAGTAACTTT ATCCGCCCCG 
CTGGAGGGCG CCAAGGACTC ACCCGTACGT CGGGCTGTGA AAGATACCCT TTCCAATCCA 
CAGTCCCCAC AGCCATCACC TTACAACTCC CCCAAACCAC AACACAAAGT CACACAGAGC 
TTCCTGCCGC CCGGCTGGGA GATGAGGATA GCGCCCAACG GCCGGCCCTT CTTCATTGAC 
CACAACACAA AGACCACGAC GTGGGAAGAT CCACGCCTGA AGTTTCCAGT ACACATGCGG 
TCAAAGGCAT CCTTAAACCC CAATGACCTT GGCCCTCTTC CTCCTGGCTG GGAAGAAAGG 
ATCCACTTGG ATGGCCGGAC GTTTTACATT GACCACAGCA GCCAGGTGTT ATGTGGCGAG 
GACGATGCCA GAGACTCAGT GCCCTCGTAC GATAGCAAAA TTACTCAGTG GGAAGACCCA 
AGACTGCAGA ACCCAGCTAT TACTGGTCCG GCTGTTCCTT ACTCCAGAGA ATTTAAGCAG 
AAATATGACT ACTTTAGAAA GAAATTAAAG AAACCTGCGG ATATTCCAAA TAGGTTTGAA 
ATGAAACTTC ACAGAAATAA CATATTTGAA GAATCCTATC GGAGAATCAT GTCTGTGAAA 
AGACCAGATG TCCTGAAAGC TAGGCTGTGG ATTGAGTTTG AATCGGAGAA AGGTCTGGAC 
TACGGGGGCG TGGCCAGAGA GTGGTTCTTC CTACTGTCCA AGGAGATGTT CAACCCTTAC 
TACGGCCTCT TCGAGTACTC GGCAACGGAC AACTACACAC TTCAGATCAA CCCCAATTCA 
GGCCTCTGTA ATGAGGACCA TCTGTCCTAT TTCACCTTCA TTGGAAGAGT TGCCGGGCTG 
GCAGTATTTC ATGGGAAACT TCTAGATGGT TTCTTCATTC GACCGTTTTA TAAAATGATG 
CTGGGAAAGC AGATAACTCT GAATGACATG GAATCTGTGG ATAGCGAATA TTACAACTCT 
TTGAAATGGA TCTTAGAAAA TGACCCAACT GAACTGGACC TTATGTTCTG CATTGATGAA 
GAGAACTTTG GGCAGACCTA TCAAGTGGAT CTGAAGCCCA ACGGGTCAGA AATAATGGTA 
ACAAATGAAA ACAAGAGAGA GTACATCGAC CTCGTTATCC AGTGGAGGTT CGTGAACAGA 
GTCCAGAAGC AGATGAATGC CTTCCTGGAG GGATTCACGG AACTGCTCCC TATTGATTTG 
ATTAAAATCT TTGATGAAAA TGAACTGGAG TTGCTGATGT GCGGCCTTGG CGATGTGGAT 
GTGAACGACT GGCGACAACA TTCTATTTAC AAGAATGGCT ACTGCCCGAA CCACCCCGTG 
ATCCAGTGGT TCTGGAAGGC TGTGCTGCTG ATGGACGCCG AGAGGCGCAT CCGGTTACTG 
CAGTTTGTCA CAGGGACGTC CCGAGTACCT ATGAATGGAT TTGCCGAGCT TTATGGTGAG 
CAGGATACCA GGGGTCTTGC CCTCCCCTCA CCCCTTGAGT GTTTACCGGG TATCAGCTGT 
GGCTCCAGAA GACTTCCCCC AGGCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128950.1
CDS	1017..4706
Translation	MAHRLRFHFGSCRSNTAPESEILDQEGEDDFFMAFHTLPRRGGAVALAPSGAQDAGLRGGVGALKRSTSMFIPQLLGPVDSRPTRSSSVQISLQRKAADGAPDDGDAGTEPPGEPLEAKSLDDGGGGGDGSPTGEPGAPGPQLNGTCGRRRAPGPDGREEAELPAVRVQHRASSADVRPVKLMPSGAEGQAGPAAAAPEPRRWSLQHVPDASGSSGKRCFVFQLQQPPASGGGPAPSGDFNFGFTGTKGDRLVRYPRIRLERSSSYPTQPRAELGSPPEERGHPDPEAPLRGRRVAPDAHRTEAVAERSPQGQGCTFKIRQDQNAGQQHFRILVTRGPEEAPQSPEEDAAAAGPASTGAGAPTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSAPAGRARSSTVTGGEAPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHSSQVLCGEDDARDSVPSYDSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGEQDTRGLALPSPLECLPGISCGSRRLPPGL

Target ORF information:

RefSeq Version	XM_025273165.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025273165.1

ORF Insert Sequence:

ATGGCCCATC GGCTTCGGTT TCATTTTGGC TCCTGCCGCA GCAACACAGC CCCCGAGTCG 
GAGATCCTAG ACCAGGAGGG AGAAGACGAC TTTTTCATGG CATTCCACAC GCTCCCGCGG 
CGGGGCGGCG CGGTCGCGCT GGCCCCGAGC GGAGCGCAGG ACGCGGGCCT GCGGGGAGGC 
GTGGGCGCGC TCAAGCGCAG CACATCCATG TTCATCCCGC AGCTGCTGGG CCCCGTGGAC 
TCGCGGCCCA CGCGGAGCTC GTCCGTGCAG ATCTCGCTGC AGCGCAAGGC GGCTGACGGC 
GCCCCTGACG ACGGCGACGC GGGCACGGAG CCGCCGGGGG AGCCCCTGGA GGCCAAGAGC 
CTCGACGACG GCGGCGGCGG CGGCGATGGC TCCCCTACCG GGGAGCCCGG GGCTCCAGGC 
CCGCAGCTCA ACGGCACGTG TGGCCGCCGC CGAGCGCCGG GCCCCGACGG CCGCGAGGAG 
GCCGAGCTGC CGGCCGTGCG TGTCCAACAC CGCGCGTCTA GCGCCGATGT GCGCCCGGTG 
AAGTTGATGC CCTCGGGAGC CGAGGGCCAG GCTGGGCCCG CAGCGGCGGC GCCCGAGCCC 
AGGCGCTGGT CCCTGCAGCA TGTTCCAGAT GCTTCTGGAA GCTCCGGGAA GCGCTGCTTT 
GTCTTCCAGC TGCAGCAGCC GCCAGCCAGT GGTGGCGGGC CTGCGCCGAG CGGCGACTTC 
AACTTCGGCT TCACCGGCAC CAAGGGGGAC AGGTTGGTGA GGTATCCCCG AATCCGGCTG 
GAGAGGAGCA GCTCGTACCC CACGCAGCCC CGGGCTGAGC TCGGGAGTCC CCCGGAGGAG 
CGCGGCCACC CAGACCCGGA GGCGCCCCTG CGGGGCCGCA GGGTGGCTCC CGATGCCCAC 
AGGACCGAGG CGGTGGCCGA GAGGTCACCA CAGGGCCAGG GCTGCACGTT TAAGATCAGG 
CAGGATCAAA ACGCGGGGCA GCAGCACTTC AGAATCCTCG TGACTCGGGG GCCCGAGGAA 
GCCCCCCAGA GTCCCGAGGA GGACGCCGCA GCGGCCGGCC CGGCTTCCAC GGGAGCCGGC 
GCCCCGACAC GGGACGACTT CCTGGGACAG GTGGACATAC CCCTTAGTCA TCTCCCGACA 
GAAGACCCAA CCATGGAGCG ACCCTACACA TTTAAGGACT TTCTCCTCCG GCCAAGAAGT 
CATAAATCTC GGGTTAAGGG ATTTTTGCGA TTGAAAATGG CCTACATGCC CAAAAACGGA 
GGCCAAGATG AAGAGAACAG TGAGCAGCGG GATGAGATGG AGCCCGGGTG GGAAGTCGTT 
GACTCCAATG ACTCGGCTTC TCAGCACCAG GAGGAGCTGC CCCCGCCTCC GCTGCCCCCC 
GGGTGGGAAG AAAAGGTGGA CAACCTGGGC CGCACGTACT ACGTGAACCA CAACAACCGG 
ACCACCCAGT GGCACCGGCC CAGCCTCATG GACGTGTCGT CCGAGTCCGA CAGCAACATC 
CGGCAGATCA ACCAGGAGGC CGCGCATCGC CGCTTCCGCT CGCGCAGACA TATCAGCGAG 
GACCTGGAGC CGGAGCCCAG CGAGGGAGGG GAGGGCCCCG AGCCTTGGGA GACCATTTCA 
GAGGAAGTGA ACATGAGCGG AGACTCGCTC AGCCTGGCGC TGCCCCCGCC ACCAGCCTCG 
CCCGTGTCCC GGACGAGCCC CCAGGAGCTG TCCGAGGAGC TGAGCAGAAG GCTGCAGGTC 
ACTCCCGACT CCAACGGCGA GCAGTTTGGG TCTTTGATTC AGCGAGAGCC GTCCTCCCGG 
CTGAGGTCCT GCAGTGTCAC TGATGCAGTT GCGGAGCAGG CTCATCTCCC GCCGCCCAGT 
GCCCCAGCTG GGAGAGCACG TTCATCAACT GTCACCGGTG GTGAGGCGCC AACGCCATCA 
GTGGCCTATG TACACACCAC GCCGGGCCTG CCCTCGGGCT GGGAAGAGAG GAAAGATGCT 
AAGGGACGCA CATACTATGT CAATCATAAC AATCGAACCA CAACTTGGAC TCGGCCTATC 
GTGCAGCTCG CAGAAGACGG CGCGTCCGGA TCCGCCACAA ACAGTAACAA CCACCTAATC 
GAGCCTCAGA TCCGCCGGCC TCGTAGCCTC AGCTCGCCAA CAGTAACTTT ATCCGCCCCG 
CTGGAGGGCG CCAAGGACTC ACCCGTACGT CGGGCTGTGA AAGATACCCT TTCCAATCCA 
CAGTCCCCAC AGCCATCACC TTACAACTCC CCCAAACCAC AACACAAAGT CACACAGAGC 
TTCCTGCCGC CCGGCTGGGA GATGAGGATA GCGCCCAACG GCCGGCCCTT CTTCATTGAC 
CACAACACAA AGACCACGAC GTGGGAAGAT CCACGCCTGA AGTTTCCAGT ACACATGCGG 
TCAAAGGCAT CCTTAAACCC CAATGACCTT GGCCCTCTTC CTCCTGGCTG GGAAGAAAGG 
ATCCACTTGG ATGGCCGGAC GTTTTACATT GACCACAGCA GCCAGGTGTT ATGTGGCGAG 
GACGATGCCA GAGACTCAGT GCCCTCGTAC GATAGCAAAA TTACTCAGTG GGAAGACCCA 
AGACTGCAGA ACCCAGCTAT TACTGGTCCG GCTGTTCCTT ACTCCAGAGA ATTTAAGCAG 
AAATATGACT ACTTTAGAAA GAAATTAAAG AAACCTGCGG ATATTCCAAA TAGGTTTGAA 
ATGAAACTTC ACAGAAATAA CATATTTGAA GAATCCTATC GGAGAATCAT GTCTGTGAAA 
AGACCAGATG TCCTGAAAGC TAGGCTGTGG ATTGAGTTTG AATCGGAGAA AGGTCTGGAC 
TACGGGGGCG TGGCCAGAGA GTGGTTCTTC CTACTGTCCA AGGAGATGTT CAACCCTTAC 
TACGGCCTCT TCGAGTACTC GGCAACGGAC AACTACACAC TTCAGATCAA CCCCAATTCA 
GGCCTCTGTA ATGAGGACCA TCTGTCCTAT TTCACCTTCA TTGGAAGAGT TGCCGGGCTG 
GCAGTATTTC ATGGGAAACT TCTAGATGGT TTCTTCATTC GACCGTTTTA TAAAATGATG 
CTGGGAAAGC AGATAACTCT GAATGACATG GAATCTGTGG ATAGCGAATA TTACAACTCT 
TTGAAATGGA TCTTAGAAAA TGACCCAACT GAACTGGACC TTATGTTCTG CATTGATGAA 
GAGAACTTTG GGCAGACCTA TCAAGTGGAT CTGAAGCCCA ACGGGTCAGA AATAATGGTA 
ACAAATGAAA ACAAGAGAGA GTACATCGAC CTCGTTATCC AGTGGAGGTT CGTGAACAGA 
GTCCAGAAGC AGATGAATGC CTTCCTGGAG GGATTCACGG AACTGCTCCC TATTGATTTG 
ATTAAAATCT TTGATGAAAA TGAACTGGAG TTGCTGATGT GCGGCCTTGG CGATGTGGAT 
GTGAACGACT GGCGACAACA TTCTATTTAC AAGAATGGCT ACTGCCCGAA CCACCCCGTG 
ATCCAGTGGT TCTGGAAGGC TGTGCTGCTG ATGGACGCCG AGAGGCGCAT CCGGTTACTG 
CAGTTTGTCA CAGGGACGTC CCGAGTACCT ATGAATGGAT TTGCCGAGCT TTATGGTGAG 
CAGGATACCA GGGGTCTTGC CCTCCCCTCA CCCCTTGAGT GTTTACCGGG TATCAGCTGT 
GGCTCCAGAA GACTTCCCCC AGGCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_025273166.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128951.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu58353
Clone ID Related Accession (Same CDS sequence)	XM_025273166.1
Accession Version	XM_025273166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCATC GGCTTCGGTT TCATTTTGGC TCCTGCCGCA GCAACACAGC CCCCGAGTCG 
GAGATCCTAG ACCAGGAGGG AGAAGACGAC TTTTTCATGG CATTCCACAC GCTCCCGCGG 
CGGGGCGGCG CGGTCGCGCT GGCCCCGAGC GGAGCGCAGG ACGCGGGCCT GCGGGGAGGC 
GTGGGCGCGC TCAAGCGCAG CACATCCATG TTCATCCCGC AGCTGCTGGG CCCCGTGGAC 
TCGCGGCCCA CGCGGAGCTC GTCCGTGCAG ATCTCGCTGC AGCGCAAGGC GGCTGACGGC 
GCCCCTGACG ACGGCGACGC GGGCACGGAG CCGCCGGGGG AGCCCCTGGA GGCCAAGAGC 
CTCGACGACG GCGGCGGCGG CGGCGATGGC TCCCCTACCG GGGAGCCCGG GGCTCCAGGC 
CCGCAGCTCA ACGGCACGTG TGGCCGCCGC CGAGCGCCGG GCCCCGACGG CCGCGAGGAG 
GCCGAGCTGC CGGCCGTGCG TGTCCAACAC CGCGCGTCTA GCGCCGATGT GCGCCCGGTG 
AAGTTGATGC CCTCGGGAGC CGAGGGCCAG GCTGGGCCCG CAGCGGCGGC GCCCGAGCCC 
AGGCGCTGGT CCCTGCAGCA TGTTCCAGAT GCTTCTGGAA GCTCCGGGAA GCGCTGCTTT 
GTCTTCCAGC TGCAGCAGCC GCCAGCCAGT GGTGGCGGGC CTGCGCCGAG CGGCGACTTC 
AACTTCGGCT TCACCGGCAC CAAGGGGGAC AGGTTGGTGA GGTATCCCCG AATCCGGCTG 
GAGAGGAGCA GCTCGTACCC CACGCAGCCC CGGGCTGAGC TCGGGAGTCC CCCGGAGGAG 
CGCGGCCACC CAGACCCGGA GGCGCCCCTG CGGGGCCGCA GGGTGGCTCC CGATGCCCAC 
AGGACCGAGG CGGTGGCCGA GAGGTCACCA CAGGGCCAGG GCTGCACGTT TAAGATCAGG 
CAGGATCAAA ACGCGGGGCA GCAGCACTTC AGAATCCTCG TGACTCGGGG GCCCGAGGAA 
GCCCCCCAGA GTCCCGAGGA GGACGCCGCA GCGGCCGGCC CGGCTTCCAC GGGAGCCGGC 
GCCCCGACAC GGGACGACTT CCTGGGACAG GTGGACATAC CCCTTAGTCA TCTCCCGACA 
GAAGACCCAA CCATGGAGCG ACCCTACACA TTTAAGGACT TTCTCCTCCG GCCAAGAAGT 
CATAAATCTC GGGTTAAGGG ATTTTTGCGA TTGAAAATGG CCTACATGCC CAAAAACGGA 
GGCCAAGATG AAGAGAACAG TGAGCAGCGG GATGAGATGG AGCCCGGGTG GGAAGTCGTT 
GACTCCAATG ACTCGGCTTC TCAGCACCAG GAGGAGCTGC CCCCGCCTCC GCTGCCCCCC 
GGGTGGGAAG AAAAGGTGGA CAACCTGGGC CGCACGTACT ACGTGAACCA CAACAACCGG 
ACCACCCAGT GGCACCGGCC CAGCCTCATG GACGTGTCGT CCGAGTCCGA CAGCAACATC 
CGGCAGATCA ACCAGGAGGC CGCGCATCGC CGCTTCCGCT CGCGCAGACA TATCAGCGAG 
GACCTGGAGC CGGAGCCCAG CGAGGGAGGG GAGGGCCCCG AGCCTTGGGA GACCATTTCA 
GAGGAAGTGA ACATGAGCGG AGACTCGCTC AGCCTGGCGC TGCCCCCGCC ACCAGCCTCG 
CCCGTGTCCC GGACGAGCCC CCAGGAGCTG TCCGAGGAGC TGAGCAGAAG GCTGCAGGTC 
ACTCCCGACT CCAACGGCGA GCAGTTTGGG TCTTTGATTC AGCGAGAGCC GTCCTCCCGG 
CTGAGGTCCT GCAGTGTCAC TGATGCAGTT GCGGAGCAGG CTCATCTCCC GCCGCCCAGT 
GCCCCAGCTG GGAGAGCACG TTCATCAACT GTCACCGGTG GTGAGGCGCC AACGCCATCA 
GTGGCCTATG TACACACCAC GCCGGGCCTG CCCTCGGGCT GGGAAGAGAG GAAAGATGCT 
AAGGGACGCA CATACTATGT CAATCATAAC AATCGAACCA CAACTTGGAC TCGGCCTATC 
GTGCAGCTCG CAGAAGACGG CGCGTCCGGA TCCGCCACAA ACAGTAACAA CCACCTAATC 
GAGCCTCAGA TCCGCCGGCC TCGTAGCCTC AGCTCGCCAA CAGTAACTTT ATCCGCCCCG 
CTGGAGGGCG CCAAGGACTC ACCCGTACGT CGGGCTGTGA AAGATACCCT TTCCAATCCA 
CAGTCCCCAC AGCCATCACC TTACAACTCC CCCAAACCAC AACACAAAGT CACACAGAGC 
TTCCTGCCGC CCGGCTGGGA GATGAGGATA GCGCCCAACG GCCGGCCCTT CTTCATTGAC 
CACAACACAA AGACCACGAC GTGGGAAGAT CCACGCCTGA AGTTTCCAGT ACACATGCGG 
TCAAAGGCAT CCTTAAACCC CAATGACCTT GGCCCTCTTC CTCCTGGCTG GGAAGAAAGG 
ATCCACTTGG ATGGCCGGAC GTTTTACATT GACCACAGCA GCCAGGTGTT ATGTGGCGAG 
GACGATGCCA GAGACTCAGT GCCCTCGTAC GATAGCAAAA TTACTCAGTG GGAAGACCCA 
AGACTGCAGA ACCCAGCTAT TACTGGTCCG GCTGTTCCTT ACTCCAGAGA ATTTAAGCAG 
AAATATGACT ACTTTAGAAA GAAATTAAAG AAACCTGCGG ATATTCCAAA TAGGTTTGAA 
ATGAAACTTC ACAGAAATAA CATATTTGAA GAATCCTATC GGAGAATCAT GTCTGTGAAA 
AGACCAGATG TCCTGAAAGC TAGGCTGTGG ATTGAGTTTG AATCGGAGAA AGGTCTGGAC 
TACGGGGGCG TGGCCAGAGA GTGGTTCTTC CTACTGTCCA AGGAGATGTT CAACCCTTAC 
TACGGCCTCT TCGAGTACTC GGCAACGGAC AACTACACAC TTCAGATCAA CCCCAATTCA 
GGCCTCTGTA ATGAGGACCA TCTGTCCTAT TTCACCTTCA TTGGAAGAGT TGCCGGGCTG 
GCAGTATTTC ATGGGAAACT TCTAGATGGT TTCTTCATTC GACCGTTTTA TAAAATGATG 
CTGGGAAAGC AGATAACTCT GAATGACATG GAATCTGTGG ATAGCGAATA TTACAACTCT 
TTGAAATGGA TCTTAGAAAA TGACCCAACT GAACTGGACC TTATGTTCTG CATTGATGAA 
GAGAACTTTG GGCAGACCTA TCAAGTGGAT CTGAAGCCCA ACGGGTCAGA AATAATGGTA 
ACAAATGAAA ACAAGAGAGA GTACATCGAC CTCGTTATCC AGTGGAGGTT CGTGAACAGA 
GTCCAGAAGC AGATGAATGC CTTCCTGGAG GGATTCACGG AACTGCTCCC TATTGATTTG 
ATTAAAATCT TTGATGAAAA TGAACTGGAG TTGCTGATGT GCGGCCTTGG CGATGTGGAT 
GTGAACGACT GGCGACAACA TTCTATTTAC AAGAATGGCT ACTGCCCGAA CCACCCCGTG 
ATCCAGTGGT TCTGGAAGGC TGTGCTGCTG ATGGACGCCG AGAGGCGCAT CCGGTTACTG 
CAGTTTGTCA CAGGGACGTC CCGAGTACCT ATGAATGGAT TTGCCGAGCT TTATGGTTCC 
AATGGTCCTC AGCTGTTTAC AATAGAGCAA TGGGGAAGTC CTGAAAAACT GCCCAGAGCT 
CACACGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128951.1
CDS	1015..4686
Translation	MAHRLRFHFGSCRSNTAPESEILDQEGEDDFFMAFHTLPRRGGAVALAPSGAQDAGLRGGVGALKRSTSMFIPQLLGPVDSRPTRSSSVQISLQRKAADGAPDDGDAGTEPPGEPLEAKSLDDGGGGGDGSPTGEPGAPGPQLNGTCGRRRAPGPDGREEAELPAVRVQHRASSADVRPVKLMPSGAEGQAGPAAAAPEPRRWSLQHVPDASGSSGKRCFVFQLQQPPASGGGPAPSGDFNFGFTGTKGDRLVRYPRIRLERSSSYPTQPRAELGSPPEERGHPDPEAPLRGRRVAPDAHRTEAVAERSPQGQGCTFKIRQDQNAGQQHFRILVTRGPEEAPQSPEEDAAAAGPASTGAGAPTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSAPAGRARSSTVTGGEAPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHSSQVLCGEDDARDSVPSYDSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTW

Target ORF information:

RefSeq Version	XM_025273166.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025273166.1

ORF Insert Sequence:

ATGGCCCATC GGCTTCGGTT TCATTTTGGC TCCTGCCGCA GCAACACAGC CCCCGAGTCG 
GAGATCCTAG ACCAGGAGGG AGAAGACGAC TTTTTCATGG CATTCCACAC GCTCCCGCGG 
CGGGGCGGCG CGGTCGCGCT GGCCCCGAGC GGAGCGCAGG ACGCGGGCCT GCGGGGAGGC 
GTGGGCGCGC TCAAGCGCAG CACATCCATG TTCATCCCGC AGCTGCTGGG CCCCGTGGAC 
TCGCGGCCCA CGCGGAGCTC GTCCGTGCAG ATCTCGCTGC AGCGCAAGGC GGCTGACGGC 
GCCCCTGACG ACGGCGACGC GGGCACGGAG CCGCCGGGGG AGCCCCTGGA GGCCAAGAGC 
CTCGACGACG GCGGCGGCGG CGGCGATGGC TCCCCTACCG GGGAGCCCGG GGCTCCAGGC 
CCGCAGCTCA ACGGCACGTG TGGCCGCCGC CGAGCGCCGG GCCCCGACGG CCGCGAGGAG 
GCCGAGCTGC CGGCCGTGCG TGTCCAACAC CGCGCGTCTA GCGCCGATGT GCGCCCGGTG 
AAGTTGATGC CCTCGGGAGC CGAGGGCCAG GCTGGGCCCG CAGCGGCGGC GCCCGAGCCC 
AGGCGCTGGT CCCTGCAGCA TGTTCCAGAT GCTTCTGGAA GCTCCGGGAA GCGCTGCTTT 
GTCTTCCAGC TGCAGCAGCC GCCAGCCAGT GGTGGCGGGC CTGCGCCGAG CGGCGACTTC 
AACTTCGGCT TCACCGGCAC CAAGGGGGAC AGGTTGGTGA GGTATCCCCG AATCCGGCTG 
GAGAGGAGCA GCTCGTACCC CACGCAGCCC CGGGCTGAGC TCGGGAGTCC CCCGGAGGAG 
CGCGGCCACC CAGACCCGGA GGCGCCCCTG CGGGGCCGCA GGGTGGCTCC CGATGCCCAC 
AGGACCGAGG CGGTGGCCGA GAGGTCACCA CAGGGCCAGG GCTGCACGTT TAAGATCAGG 
CAGGATCAAA ACGCGGGGCA GCAGCACTTC AGAATCCTCG TGACTCGGGG GCCCGAGGAA 
GCCCCCCAGA GTCCCGAGGA GGACGCCGCA GCGGCCGGCC CGGCTTCCAC GGGAGCCGGC 
GCCCCGACAC GGGACGACTT CCTGGGACAG GTGGACATAC CCCTTAGTCA TCTCCCGACA 
GAAGACCCAA CCATGGAGCG ACCCTACACA TTTAAGGACT TTCTCCTCCG GCCAAGAAGT 
CATAAATCTC GGGTTAAGGG ATTTTTGCGA TTGAAAATGG CCTACATGCC CAAAAACGGA 
GGCCAAGATG AAGAGAACAG TGAGCAGCGG GATGAGATGG AGCCCGGGTG GGAAGTCGTT 
GACTCCAATG ACTCGGCTTC TCAGCACCAG GAGGAGCTGC CCCCGCCTCC GCTGCCCCCC 
GGGTGGGAAG AAAAGGTGGA CAACCTGGGC CGCACGTACT ACGTGAACCA CAACAACCGG 
ACCACCCAGT GGCACCGGCC CAGCCTCATG GACGTGTCGT CCGAGTCCGA CAGCAACATC 
CGGCAGATCA ACCAGGAGGC CGCGCATCGC CGCTTCCGCT CGCGCAGACA TATCAGCGAG 
GACCTGGAGC CGGAGCCCAG CGAGGGAGGG GAGGGCCCCG AGCCTTGGGA GACCATTTCA 
GAGGAAGTGA ACATGAGCGG AGACTCGCTC AGCCTGGCGC TGCCCCCGCC ACCAGCCTCG 
CCCGTGTCCC GGACGAGCCC CCAGGAGCTG TCCGAGGAGC TGAGCAGAAG GCTGCAGGTC 
ACTCCCGACT CCAACGGCGA GCAGTTTGGG TCTTTGATTC AGCGAGAGCC GTCCTCCCGG 
CTGAGGTCCT GCAGTGTCAC TGATGCAGTT GCGGAGCAGG CTCATCTCCC GCCGCCCAGT 
GCCCCAGCTG GGAGAGCACG TTCATCAACT GTCACCGGTG GTGAGGCGCC AACGCCATCA 
GTGGCCTATG TACACACCAC GCCGGGCCTG CCCTCGGGCT GGGAAGAGAG GAAAGATGCT 
AAGGGACGCA CATACTATGT CAATCATAAC AATCGAACCA CAACTTGGAC TCGGCCTATC 
GTGCAGCTCG CAGAAGACGG CGCGTCCGGA TCCGCCACAA ACAGTAACAA CCACCTAATC 
GAGCCTCAGA TCCGCCGGCC TCGTAGCCTC AGCTCGCCAA CAGTAACTTT ATCCGCCCCG 
CTGGAGGGCG CCAAGGACTC ACCCGTACGT CGGGCTGTGA AAGATACCCT TTCCAATCCA 
CAGTCCCCAC AGCCATCACC TTACAACTCC CCCAAACCAC AACACAAAGT CACACAGAGC 
TTCCTGCCGC CCGGCTGGGA GATGAGGATA GCGCCCAACG GCCGGCCCTT CTTCATTGAC 
CACAACACAA AGACCACGAC GTGGGAAGAT CCACGCCTGA AGTTTCCAGT ACACATGCGG 
TCAAAGGCAT CCTTAAACCC CAATGACCTT GGCCCTCTTC CTCCTGGCTG GGAAGAAAGG 
ATCCACTTGG ATGGCCGGAC GTTTTACATT GACCACAGCA GCCAGGTGTT ATGTGGCGAG 
GACGATGCCA GAGACTCAGT GCCCTCGTAC GATAGCAAAA TTACTCAGTG GGAAGACCCA 
AGACTGCAGA ACCCAGCTAT TACTGGTCCG GCTGTTCCTT ACTCCAGAGA ATTTAAGCAG 
AAATATGACT ACTTTAGAAA GAAATTAAAG AAACCTGCGG ATATTCCAAA TAGGTTTGAA 
ATGAAACTTC ACAGAAATAA CATATTTGAA GAATCCTATC GGAGAATCAT GTCTGTGAAA 
AGACCAGATG TCCTGAAAGC TAGGCTGTGG ATTGAGTTTG AATCGGAGAA AGGTCTGGAC 
TACGGGGGCG TGGCCAGAGA GTGGTTCTTC CTACTGTCCA AGGAGATGTT CAACCCTTAC 
TACGGCCTCT TCGAGTACTC GGCAACGGAC AACTACACAC TTCAGATCAA CCCCAATTCA 
GGCCTCTGTA ATGAGGACCA TCTGTCCTAT TTCACCTTCA TTGGAAGAGT TGCCGGGCTG 
GCAGTATTTC ATGGGAAACT TCTAGATGGT TTCTTCATTC GACCGTTTTA TAAAATGATG 
CTGGGAAAGC AGATAACTCT GAATGACATG GAATCTGTGG ATAGCGAATA TTACAACTCT 
TTGAAATGGA TCTTAGAAAA TGACCCAACT GAACTGGACC TTATGTTCTG CATTGATGAA 
GAGAACTTTG GGCAGACCTA TCAAGTGGAT CTGAAGCCCA ACGGGTCAGA AATAATGGTA 
ACAAATGAAA ACAAGAGAGA GTACATCGAC CTCGTTATCC AGTGGAGGTT CGTGAACAGA 
GTCCAGAAGC AGATGAATGC CTTCCTGGAG GGATTCACGG AACTGCTCCC TATTGATTTG 
ATTAAAATCT TTGATGAAAA TGAACTGGAG TTGCTGATGT GCGGCCTTGG CGATGTGGAT 
GTGAACGACT GGCGACAACA TTCTATTTAC AAGAATGGCT ACTGCCCGAA CCACCCCGTG 
ATCCAGTGGT TCTGGAAGGC TGTGCTGCTG ATGGACGCCG AGAGGCGCAT CCGGTTACTG 
CAGTTTGTCA CAGGGACGTC CCGAGTACCT ATGAATGGAT TTGCCGAGCT TTATGGTTCC 
AATGGTCCTC AGCTGTTTAC AATAGAGCAA TGGGGAAGTC CTGAAAAACT GCCCAGAGCT 
CACACGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073366.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073428.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA.

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CloneID	OBu58354
Clone ID Related Accession (Same CDS sequence)	XM_006073366.2
Accession Version	XM_006073366.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006073366.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGAGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGCC ATCAGTGGCC 
TATGTACACA CCACGCCGGG CCTGCCCTCG GGCTGGGAAG AGAGGAAAGA TGCTAAGGGA 
CGCACATACT ATGTCAATCA TAACAATCGA ACCACAACTT GGACTCGGCC TATCGTGCAG 
CTCGCAGAAG ACGGCGCGTC CGGATCCGCC ACAAACAGTA ACAACCACCT AATCGAGCCT 
CAGATCCGCC GGCCTCGTAG CCTCAGCTCG CCAACAGTAA CTTTATCCGC CCCGCTGGAG 
GGCGCCAAGG ACTCACCCGT ACGTCGGGCT GTGAAAGATA CCCTTTCCAA TCCACAGTCC 
CCACAGCCAT CACCTTACAA CTCCCCCAAA CCACAACACA AAGTCACACA GAGCTTCCTG 
CCGCCCGGCT GGGAGATGAG GATAGCGCCC AACGGCCGGC CCTTCTTCAT TGACCACAAC 
ACAAAGACCA CGACGTGGGA AGATCCACGC CTGAAGTTTC CAGTACACAT GCGGTCAAAG 
GCATCCTTAA ACCCCAATGA CCTTGGCCCT CTTCCTCCTG GCTGGGAAGA AAGGATCCAC 
TTGGATGGCC GGACGTTTTA CATTGACCAC AATAGCAAAA TTACTCAGTG GGAAGACCCA 
AGACTGCAGA ACCCAGCTAT TACTGGTCCG GCTGTTCCTT ACTCCAGAGA ATTTAAGCAG 
AAATATGACT ACTTTAGAAA GAAATTAAAG AAACCTGCGG ATATTCCAAA TAGGTTTGAA 
ATGAAACTTC ACAGAAATAA CATATTTGAA GAATCCTATC GGAGAATCAT GTCTGTGAAA 
AGACCAGATG TCCTGAAAGC TAGGCTGTGG ATTGAGTTTG AATCGGAGAA AGGTCTGGAC 
TACGGGGGCG TGGCCAGAGA GTGGTTCTTC CTACTGTCCA AGGAGATGTT CAACCCTTAC 
TACGGCCTCT TCGAGTACTC GGCAACGGAC AACTACACAC TTCAGATCAA CCCCAATTCA 
GGCCTCTGTA ATGAGGACCA TCTGTCCTAT TTCACCTTCA TTGGAAGAGT TGCCGGGCTG 
GCAGTATTTC ATGGGAAACT TCTAGATGGT TTCTTCATTC GACCGTTTTA TAAAATGATG 
CTGGGAAAGC AGATAACTCT GAATGACATG GAATCTGTGG ATAGCGAATA TTACAACTCT 
TTGAAATGGA TCTTAGAAAA TGACCCAACT GAACTGGACC TTATGTTCTG CATTGATGAA 
GAGAACTTTG GGCAGACCTA TCAAGTGGAT CTGAAGCCCA ACGGGTCAGA AATAATGGTA 
ACAAATGAAA ACAAGAGAGA GTACATCGAC CTCGTTATCC AGTGGAGGTT CGTGAACAGA 
GTCCAGAAGC AGATGAATGC CTTCCTGGAG GGATTCACGG AACTGCTCCC TATTGATTTG 
ATTAAAATCT TTGATGAAAA TGAACTGGAG TTGCTGATGT GCGGCCTTGG CGATGTGGAT 
GTGAACGACT GGCGACAACA TTCTATTTAC AAGAATGGCT ACTGCCCGAA CCACCCCGTG 
ATCCAGTGGT TCTGGAAGGC TGTGCTGCTG ATGGACGCCG AGAGGCGCAT CCGGTTACTG 
CAGTTTGTCA CAGGGACGTC CCGAGTACCT ATGAATGGAT TTGCCGAGCT TTATGGTTCC 
AATGGTCCTC AGCTGTTTAC AATAGAGCAA TGGGGAAGTC CTGAAAAACT GCCCAGAGCT 
CACACGTGCT TTAATCGCCT TGACTTACCT CCGTATGAAA CCTTCGAAGA TTTACGAGAG 
AAGCTCCTCA TGGCTGTGGA AAACGCCCAA GGCTTCGAGG GGGTGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073428.1
CDS	78..3188
Translation	MPSGAEGQAGPAAAAPEPRRWSLQHVPDASGSSGKRCFVFQLQQPPASGGGPAPSGDFNFGFTGTKGDRLVRYPRIRLERSSSYPTQPRAELGSPPEERGHPDPEAPLRGRRVAPDAHRTEAVAERSPQGQGCTFKIRQDQNAGQQHFRILVTRGPEEAPQSPEEDAAAAGPASTGAGAPTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073366.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073366.2

ORF Insert Sequence:

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGAGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGCC ATCAGTGGCC 
TATGTACACA CCACGCCGGG CCTGCCCTCG GGCTGGGAAG AGAGGAAAGA TGCTAAGGGA 
CGCACATACT ATGTCAATCA TAACAATCGA ACCACAACTT GGACTCGGCC TATCGTGCAG 
CTCGCAGAAG ACGGCGCGTC CGGATCCGCC ACAAACAGTA ACAACCACCT AATCGAGCCT 
CAGATCCGCC GGCCTCGTAG CCTCAGCTCG CCAACAGTAA CTTTATCCGC CCCGCTGGAG 
GGCGCCAAGG ACTCACCCGT ACGTCGGGCT GTGAAAGATA CCCTTTCCAA TCCACAGTCC 
CCACAGCCAT CACCTTACAA CTCCCCCAAA CCACAACACA AAGTCACACA GAGCTTCCTG 
CCGCCCGGCT GGGAGATGAG GATAGCGCCC AACGGCCGGC CCTTCTTCAT TGACCACAAC 
ACAAAGACCA CGACGTGGGA AGATCCACGC CTGAAGTTTC CAGTACACAT GCGGTCAAAG 
GCATCCTTAA ACCCCAATGA CCTTGGCCCT CTTCCTCCTG GCTGGGAAGA AAGGATCCAC 
TTGGATGGCC GGACGTTTTA CATTGACCAC AATAGCAAAA TTACTCAGTG GGAAGACCCA 
AGACTGCAGA ACCCAGCTAT TACTGGTCCG GCTGTTCCTT ACTCCAGAGA ATTTAAGCAG 
AAATATGACT ACTTTAGAAA GAAATTAAAG AAACCTGCGG ATATTCCAAA TAGGTTTGAA 
ATGAAACTTC ACAGAAATAA CATATTTGAA GAATCCTATC GGAGAATCAT GTCTGTGAAA 
AGACCAGATG TCCTGAAAGC TAGGCTGTGG ATTGAGTTTG AATCGGAGAA AGGTCTGGAC 
TACGGGGGCG TGGCCAGAGA GTGGTTCTTC CTACTGTCCA AGGAGATGTT CAACCCTTAC 
TACGGCCTCT TCGAGTACTC GGCAACGGAC AACTACACAC TTCAGATCAA CCCCAATTCA 
GGCCTCTGTA ATGAGGACCA TCTGTCCTAT TTCACCTTCA TTGGAAGAGT TGCCGGGCTG 
GCAGTATTTC ATGGGAAACT TCTAGATGGT TTCTTCATTC GACCGTTTTA TAAAATGATG 
CTGGGAAAGC AGATAACTCT GAATGACATG GAATCTGTGG ATAGCGAATA TTACAACTCT 
TTGAAATGGA TCTTAGAAAA TGACCCAACT GAACTGGACC TTATGTTCTG CATTGATGAA 
GAGAACTTTG GGCAGACCTA TCAAGTGGAT CTGAAGCCCA ACGGGTCAGA AATAATGGTA 
ACAAATGAAA ACAAGAGAGA GTACATCGAC CTCGTTATCC AGTGGAGGTT CGTGAACAGA 
GTCCAGAAGC AGATGAATGC CTTCCTGGAG GGATTCACGG AACTGCTCCC TATTGATTTG 
ATTAAAATCT TTGATGAAAA TGAACTGGAG TTGCTGATGT GCGGCCTTGG CGATGTGGAT 
GTGAACGACT GGCGACAACA TTCTATTTAC AAGAATGGCT ACTGCCCGAA CCACCCCGTG 
ATCCAGTGGT TCTGGAAGGC TGTGCTGCTG ATGGACGCCG AGAGGCGCAT CCGGTTACTG 
CAGTTTGTCA CAGGGACGTC CCGAGTACCT ATGAATGGAT TTGCCGAGCT TTATGGTTCC 
AATGGTCCTC AGCTGTTTAC AATAGAGCAA TGGGGAAGTC CTGAAAAACT GCCCAGAGCT 
CACACGTGCT TTAATCGCCT TGACTTACCT CCGTATGAAA CCTTCGAAGA TTTACGAGAG 
AAGCTCCTCA TGGCTGTGGA AAACGCCCAA GGCTTCGAGG GGGTGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073363.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073425.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.

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CloneID	OBu27437
Clone ID Related Accession (Same CDS sequence)	XM_006073363.1
Accession Version	XM_006073363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785536.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGGGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGCC CAGTGCCCCA 
GCTGGGAGAG CACGTTCATC AACTGTCACC GGTGGTGAGG CGCCAACGCC ATCAGTGGCC 
TATGTACACA CCACGCCGGG CCTGCCCTCG GGCTGGGAAG AGAGGAAAGA TGCTAAGGGA 
CGCACATACT ATGTCAATCA TAACAATCGA ACCACAACTT GGACTCGGCC TATCGTGCAG 
CTCGCAGAAG ACGGCGCGTC CGGATCCGCC ACAAACAGTA ACAACCACCT AATCGAGCCT 
CAGATCCGCC GGCCTCGTAG CCTCAGCTCG CCAACAGTAA CTTTATCCGC CCCGCTGGAG 
GGCGCCAAGG ACTCACCCGT ACGTCGGGCT GTGAAAGATA CCCTTTCCAA TCCACAGTCC 
CCACAGCCAT CACCTTACAA CTCCCCCAAA CCACAACACA AAGTCACACA GAGCTTCCTG 
CCGCCCGGCT GGGAGATGAG GATAGCGCCC AACGGCCGGC CCTTCTTCAT TGACCACAAC 
ACAAAGACCA CGACGTGGGA AGATCCACGC CTGAAGTTTC CAGTACACAT GCGGTCAAAG 
GCATCCTTAA ACCCCAATGA CCTTGGCCCT CTTCCTCCTG GCTGGGAAGA AAGGATCCAC 
TTGGATGGCC GGACGTTTTA CATTGACCAC AGCAGCCAGG TGTTATGTGG CGAGGACGAT 
GCCAGAGACT CAGTGCCCTC GTACGATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG 
CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT 
GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA 
CTTCACAGAA ATAACATATT TGAAGAATCC TATCGGAGAA TCATGTCTGT GAAAAGACCA 
GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG 
GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC 
CTCTTCGAGT ACTCGGCAAC GGACAACTAC ACACTTCAGA TCAACCCCAA TTCAGGCCTC 
TGTAATGAGG ACCATCTGTC CTATTTCACC TTCATTGGAA GAGTTGCCGG GCTGGCAGTA 
TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA 
AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA 
TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC 
TTTGGGCAGA CCTATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACAAAT 
GAAAACAAGA GAGAGTACAT CGACCTCGTT ATCCAGTGGA GGTTCGTGAA CAGAGTCCAG 
AAGCAGATGA ATGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA 
ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGCGGCC TTGGCGATGT GGATGTGAAC 
GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG 
TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAGGC GCATCCGGTT ACTGCAGTTT 
GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT 
CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG 
TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC 
CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073425.1
CDS	67..3291
Translation	MPSGAEGQAGPAAAAPEPRRWSLQHVPDASGSSGKRCFVFQLQQPPASGGGPAPSGDFNFGFTGTKGDRLVRYPRIRLERSSSYPTQPRAELGSPPEERGHPDPEAPLRGRRVAPDAHRTEAVAERSPQGQGCTFKIRQDQNAGQQHFRILVTRGPEEAPQSPEEDAAAAGPASTGAGAPTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSAPAGRARSSTVTGGEAPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHSSQVLCGEDDARDSVPSYDSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073363.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073363.1

ORF Insert Sequence:

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGGGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGCC CAGTGCCCCA 
GCTGGGAGAG CACGTTCATC AACTGTCACC GGTGGTGAGG CGCCAACGCC ATCAGTGGCC 
TATGTACACA CCACGCCGGG CCTGCCCTCG GGCTGGGAAG AGAGGAAAGA TGCTAAGGGA 
CGCACATACT ATGTCAATCA TAACAATCGA ACCACAACTT GGACTCGGCC TATCGTGCAG 
CTCGCAGAAG ACGGCGCGTC CGGATCCGCC ACAAACAGTA ACAACCACCT AATCGAGCCT 
CAGATCCGCC GGCCTCGTAG CCTCAGCTCG CCAACAGTAA CTTTATCCGC CCCGCTGGAG 
GGCGCCAAGG ACTCACCCGT ACGTCGGGCT GTGAAAGATA CCCTTTCCAA TCCACAGTCC 
CCACAGCCAT CACCTTACAA CTCCCCCAAA CCACAACACA AAGTCACACA GAGCTTCCTG 
CCGCCCGGCT GGGAGATGAG GATAGCGCCC AACGGCCGGC CCTTCTTCAT TGACCACAAC 
ACAAAGACCA CGACGTGGGA AGATCCACGC CTGAAGTTTC CAGTACACAT GCGGTCAAAG 
GCATCCTTAA ACCCCAATGA CCTTGGCCCT CTTCCTCCTG GCTGGGAAGA AAGGATCCAC 
TTGGATGGCC GGACGTTTTA CATTGACCAC AGCAGCCAGG TGTTATGTGG CGAGGACGAT 
GCCAGAGACT CAGTGCCCTC GTACGATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG 
CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT 
GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA 
CTTCACAGAA ATAACATATT TGAAGAATCC TATCGGAGAA TCATGTCTGT GAAAAGACCA 
GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG 
GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC 
CTCTTCGAGT ACTCGGCAAC GGACAACTAC ACACTTCAGA TCAACCCCAA TTCAGGCCTC 
TGTAATGAGG ACCATCTGTC CTATTTCACC TTCATTGGAA GAGTTGCCGG GCTGGCAGTA 
TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA 
AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA 
TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC 
TTTGGGCAGA CCTATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACAAAT 
GAAAACAAGA GAGAGTACAT CGACCTCGTT ATCCAGTGGA GGTTCGTGAA CAGAGTCCAG 
AAGCAGATGA ATGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA 
ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGCGGCC TTGGCGATGT GGATGTGAAC 
GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG 
TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAGGC GCATCCGGTT ACTGCAGTTT 
GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT 
CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG 
TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC 
CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073364.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073426.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.

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CloneID	OBu27438
Clone ID Related Accession (Same CDS sequence)	XM_006073364.1
Accession Version	XM_006073364.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785536.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGGGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGCC CAGTGCCCCA 
GCTGGGAGAG CACGTTCATC AACTGTCACC GGTGGTGAGG CGCCAACGCC ATCAGTGGCC 
TATGTACACA CCACGCCGGG CCTGCCCTCG GGCTGGGAAG AGAGGAAAGA TGCTAAGGGA 
CGCACATACT ATGTCAATCA TAACAATCGA ACCACAACTT GGACTCGGCC TATCGTGCAG 
CTCGCAGAAG ACGGCGCGTC CGGATCCGCC ACAAACAGTA ACAACCACCT AATCGAGCCT 
CAGATCCGCC GGCCTCGTAG CCTCAGCTCG CCAACAGTAA CTTTATCCGC CCCGCTGGAG 
GGCGCCAAGG ACTCACCCGT ACGTCGGGCT GTGAAAGATA CCCTTTCCAA TCCACAGTCC 
CCACAGCCAT CACCTTACAA CTCCCCCAAA CCACAACACA AAGTCACACA GAGCTTCCTG 
CCGCCCGGCT GGGAGATGAG GATAGCGCCC AACGGCCGGC CCTTCTTCAT TGACCACAAC 
ACAAAGACCA CGACGTGGGA AGATCCACGC CTGAAGTTTC CAGTACACAT GCGGTCAAAG 
GCATCCTTAA ACCCCAATGA CCTTGGCCCT CTTCCTCCTG GCTGGGAAGA AAGGATCCAC 
TTGGATGGCC GGACGTTTTA CATTGACCAC AATAGCAAAA TTACTCAGTG GGAAGACCCA 
AGACTGCAGA ACCCAGCTAT TACTGGTCCG GCTGTTCCTT ACTCCAGAGA ATTTAAGCAG 
AAATATGACT ACTTTAGAAA GAAATTAAAG AAACCTGCGG ATATTCCAAA TAGGTTTGAA 
ATGAAACTTC ACAGAAATAA CATATTTGAA GAATCCTATC GGAGAATCAT GTCTGTGAAA 
AGACCAGATG TCCTGAAAGC TAGGCTGTGG ATTGAGTTTG AATCGGAGAA AGGTCTGGAC 
TACGGGGGCG TGGCCAGAGA GTGGTTCTTC CTACTGTCCA AGGAGATGTT CAACCCTTAC 
TACGGCCTCT TCGAGTACTC GGCAACGGAC AACTACACAC TTCAGATCAA CCCCAATTCA 
GGCCTCTGTA ATGAGGACCA TCTGTCCTAT TTCACCTTCA TTGGAAGAGT TGCCGGGCTG 
GCAGTATTTC ATGGGAAACT TCTAGATGGT TTCTTCATTC GACCGTTTTA TAAAATGATG 
CTGGGAAAGC AGATAACTCT GAATGACATG GAATCTGTGG ATAGCGAATA TTACAACTCT 
TTGAAATGGA TCTTAGAAAA TGACCCAACT GAACTGGACC TTATGTTCTG CATTGATGAA 
GAGAACTTTG GGCAGACCTA TCAAGTGGAT CTGAAGCCCA ACGGGTCAGA AATAATGGTA 
ACAAATGAAA ACAAGAGAGA GTACATCGAC CTCGTTATCC AGTGGAGGTT CGTGAACAGA 
GTCCAGAAGC AGATGAATGC CTTCCTGGAG GGATTCACGG AACTGCTCCC TATTGATTTG 
ATTAAAATCT TTGATGAAAA TGAACTGGAG TTGCTGATGT GCGGCCTTGG CGATGTGGAT 
GTGAACGACT GGCGACAACA TTCTATTTAC AAGAATGGCT ACTGCCCGAA CCACCCCGTG 
ATCCAGTGGT TCTGGAAGGC TGTGCTGCTG ATGGACGCCG AGAGGCGCAT CCGGTTACTG 
CAGTTTGTCA CAGGGACGTC CCGAGTACCT ATGAATGGAT TTGCCGAGCT TTATGGTTCC 
AATGGTCCTC AGCTGTTTAC AATAGAGCAA TGGGGAAGTC CTGAAAAACT GCCCAGAGCT 
CACACGTGCT TTAATCGCCT TGACTTACCT CCGTATGAAA CCTTCGAAGA TTTACGAGAG 
AAGCTCCTCA TGGCTGTGGA AAACGCCCAA GGCTTCGAGG GGGTGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073426.1
CDS	66..3236
Translation	MPSGAEGQAGPAAAAPEPRRWSLQHVPDASGSSGKRCFVFQLQQPPASGGGPAPSGDFNFGFTGTKGDRLVRYPRIRLERSSSYPTQPRAELGSPPEERGHPDPEAPLRGRRVAPDAHRTEAVAERSPQGQGCTFKIRQDQNAGQQHFRILVTRGPEEAPQSPEEDAAAAGPASTGAGAPTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSAPAGRARSSTVTGGEAPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073364.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073364.1

ORF Insert Sequence:

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGGGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGCC CAGTGCCCCA 
GCTGGGAGAG CACGTTCATC AACTGTCACC GGTGGTGAGG CGCCAACGCC ATCAGTGGCC 
TATGTACACA CCACGCCGGG CCTGCCCTCG GGCTGGGAAG AGAGGAAAGA TGCTAAGGGA 
CGCACATACT ATGTCAATCA TAACAATCGA ACCACAACTT GGACTCGGCC TATCGTGCAG 
CTCGCAGAAG ACGGCGCGTC CGGATCCGCC ACAAACAGTA ACAACCACCT AATCGAGCCT 
CAGATCCGCC GGCCTCGTAG CCTCAGCTCG CCAACAGTAA CTTTATCCGC CCCGCTGGAG 
GGCGCCAAGG ACTCACCCGT ACGTCGGGCT GTGAAAGATA CCCTTTCCAA TCCACAGTCC 
CCACAGCCAT CACCTTACAA CTCCCCCAAA CCACAACACA AAGTCACACA GAGCTTCCTG 
CCGCCCGGCT GGGAGATGAG GATAGCGCCC AACGGCCGGC CCTTCTTCAT TGACCACAAC 
ACAAAGACCA CGACGTGGGA AGATCCACGC CTGAAGTTTC CAGTACACAT GCGGTCAAAG 
GCATCCTTAA ACCCCAATGA CCTTGGCCCT CTTCCTCCTG GCTGGGAAGA AAGGATCCAC 
TTGGATGGCC GGACGTTTTA CATTGACCAC AATAGCAAAA TTACTCAGTG GGAAGACCCA 
AGACTGCAGA ACCCAGCTAT TACTGGTCCG GCTGTTCCTT ACTCCAGAGA ATTTAAGCAG 
AAATATGACT ACTTTAGAAA GAAATTAAAG AAACCTGCGG ATATTCCAAA TAGGTTTGAA 
ATGAAACTTC ACAGAAATAA CATATTTGAA GAATCCTATC GGAGAATCAT GTCTGTGAAA 
AGACCAGATG TCCTGAAAGC TAGGCTGTGG ATTGAGTTTG AATCGGAGAA AGGTCTGGAC 
TACGGGGGCG TGGCCAGAGA GTGGTTCTTC CTACTGTCCA AGGAGATGTT CAACCCTTAC 
TACGGCCTCT TCGAGTACTC GGCAACGGAC AACTACACAC TTCAGATCAA CCCCAATTCA 
GGCCTCTGTA ATGAGGACCA TCTGTCCTAT TTCACCTTCA TTGGAAGAGT TGCCGGGCTG 
GCAGTATTTC ATGGGAAACT TCTAGATGGT TTCTTCATTC GACCGTTTTA TAAAATGATG 
CTGGGAAAGC AGATAACTCT GAATGACATG GAATCTGTGG ATAGCGAATA TTACAACTCT 
TTGAAATGGA TCTTAGAAAA TGACCCAACT GAACTGGACC TTATGTTCTG CATTGATGAA 
GAGAACTTTG GGCAGACCTA TCAAGTGGAT CTGAAGCCCA ACGGGTCAGA AATAATGGTA 
ACAAATGAAA ACAAGAGAGA GTACATCGAC CTCGTTATCC AGTGGAGGTT CGTGAACAGA 
GTCCAGAAGC AGATGAATGC CTTCCTGGAG GGATTCACGG AACTGCTCCC TATTGATTTG 
ATTAAAATCT TTGATGAAAA TGAACTGGAG TTGCTGATGT GCGGCCTTGG CGATGTGGAT 
GTGAACGACT GGCGACAACA TTCTATTTAC AAGAATGGCT ACTGCCCGAA CCACCCCGTG 
ATCCAGTGGT TCTGGAAGGC TGTGCTGCTG ATGGACGCCG AGAGGCGCAT CCGGTTACTG 
CAGTTTGTCA CAGGGACGTC CCGAGTACCT ATGAATGGAT TTGCCGAGCT TTATGGTTCC 
AATGGTCCTC AGCTGTTTAC AATAGAGCAA TGGGGAAGTC CTGAAAAACT GCCCAGAGCT 
CACACGTGCT TTAATCGCCT TGACTTACCT CCGTATGAAA CCTTCGAAGA TTTACGAGAG 
AAGCTCCTCA TGGCTGTGGA AAACGCCCAA GGCTTCGAGG GGGTGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073365.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073427.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu27439
Clone ID Related Accession (Same CDS sequence)	XM_006073365.1
Accession Version	XM_006073365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785536.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGGGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGCC ATCAGTGGCC 
TATGTACACA CCACGCCGGG CCTGCCCTCG GGCTGGGAAG AGAGGAAAGA TGCTAAGGGA 
CGCACATACT ATGTCAATCA TAACAATCGA ACCACAACTT GGACTCGGCC TATCGTGCAG 
CTCGCAGAAG ACGGCGCGTC CGGATCCGCC ACAAACAGTA ACAACCACCT AATCGAGCCT 
CAGATCCGCC GGCCTCGTAG CCTCAGCTCG CCAACAGTAA CTTTATCCGC CCCGCTGGAG 
GGCGCCAAGG ACTCACCCGT ACGTCGGGCT GTGAAAGATA CCCTTTCCAA TCCACAGTCC 
CCACAGCCAT CACCTTACAA CTCCCCCAAA CCACAACACA AAGTCACACA GAGCTTCCTG 
CCGCCCGGCT GGGAGATGAG GATAGCGCCC AACGGCCGGC CCTTCTTCAT TGACCACAAC 
ACAAAGACCA CGACGTGGGA AGATCCACGC CTGAAGTTTC CAGTACACAT GCGGTCAAAG 
GCATCCTTAA ACCCCAATGA CCTTGGCCCT CTTCCTCCTG GCTGGGAAGA AAGGATCCAC 
TTGGATGGCC GGACGTTTTA CATTGACCAC AGCAGCCAGG TGTTATGTGG CGAGGACGAT 
GCCAGAGACT CAGTGCCCTC GTACGATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG 
CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT 
GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA 
CTTCACAGAA ATAACATATT TGAAGAATCC TATCGGAGAA TCATGTCTGT GAAAAGACCA 
GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG 
GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC 
CTCTTCGAGT ACTCGGCAAC GGACAACTAC ACACTTCAGA TCAACCCCAA TTCAGGCCTC 
TGTAATGAGG ACCATCTGTC CTATTTCACC TTCATTGGAA GAGTTGCCGG GCTGGCAGTA 
TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA 
AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA 
TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC 
TTTGGGCAGA CCTATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACAAAT 
GAAAACAAGA GAGAGTACAT CGACCTCGTT ATCCAGTGGA GGTTCGTGAA CAGAGTCCAG 
AAGCAGATGA ATGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA 
ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGCGGCC TTGGCGATGT GGATGTGAAC 
GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG 
TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAGGC GCATCCGGTT ACTGCAGTTT 
GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT 
CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG 
TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC 
CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073427.1
CDS	67..3231
Translation	MPSGAEGQAGPAAAAPEPRRWSLQHVPDASGSSGKRCFVFQLQQPPASGGGPAPSGDFNFGFTGTKGDRLVRYPRIRLERSSSYPTQPRAELGSPPEERGHPDPEAPLRGRRVAPDAHRTEAVAERSPQGQGCTFKIRQDQNAGQQHFRILVTRGPEEAPQSPEEDAAAAGPASTGAGAPTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHSSQVLCGEDDARDSVPSYDSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073365.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073365.1

ORF Insert Sequence:

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGGGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGCC ATCAGTGGCC 
TATGTACACA CCACGCCGGG CCTGCCCTCG GGCTGGGAAG AGAGGAAAGA TGCTAAGGGA 
CGCACATACT ATGTCAATCA TAACAATCGA ACCACAACTT GGACTCGGCC TATCGTGCAG 
CTCGCAGAAG ACGGCGCGTC CGGATCCGCC ACAAACAGTA ACAACCACCT AATCGAGCCT 
CAGATCCGCC GGCCTCGTAG CCTCAGCTCG CCAACAGTAA CTTTATCCGC CCCGCTGGAG 
GGCGCCAAGG ACTCACCCGT ACGTCGGGCT GTGAAAGATA CCCTTTCCAA TCCACAGTCC 
CCACAGCCAT CACCTTACAA CTCCCCCAAA CCACAACACA AAGTCACACA GAGCTTCCTG 
CCGCCCGGCT GGGAGATGAG GATAGCGCCC AACGGCCGGC CCTTCTTCAT TGACCACAAC 
ACAAAGACCA CGACGTGGGA AGATCCACGC CTGAAGTTTC CAGTACACAT GCGGTCAAAG 
GCATCCTTAA ACCCCAATGA CCTTGGCCCT CTTCCTCCTG GCTGGGAAGA AAGGATCCAC 
TTGGATGGCC GGACGTTTTA CATTGACCAC AGCAGCCAGG TGTTATGTGG CGAGGACGAT 
GCCAGAGACT CAGTGCCCTC GTACGATAGC AAAATTACTC AGTGGGAAGA CCCAAGACTG 
CAGAACCCAG CTATTACTGG TCCGGCTGTT CCTTACTCCA GAGAATTTAA GCAGAAATAT 
GACTACTTTA GAAAGAAATT AAAGAAACCT GCGGATATTC CAAATAGGTT TGAAATGAAA 
CTTCACAGAA ATAACATATT TGAAGAATCC TATCGGAGAA TCATGTCTGT GAAAAGACCA 
GATGTCCTGA AAGCTAGGCT GTGGATTGAG TTTGAATCGG AGAAAGGTCT GGACTACGGG 
GGCGTGGCCA GAGAGTGGTT CTTCCTACTG TCCAAGGAGA TGTTCAACCC TTACTACGGC 
CTCTTCGAGT ACTCGGCAAC GGACAACTAC ACACTTCAGA TCAACCCCAA TTCAGGCCTC 
TGTAATGAGG ACCATCTGTC CTATTTCACC TTCATTGGAA GAGTTGCCGG GCTGGCAGTA 
TTTCATGGGA AACTTCTAGA TGGTTTCTTC ATTCGACCGT TTTATAAAAT GATGCTGGGA 
AAGCAGATAA CTCTGAATGA CATGGAATCT GTGGATAGCG AATATTACAA CTCTTTGAAA 
TGGATCTTAG AAAATGACCC AACTGAACTG GACCTTATGT TCTGCATTGA TGAAGAGAAC 
TTTGGGCAGA CCTATCAAGT GGATCTGAAG CCCAACGGGT CAGAAATAAT GGTAACAAAT 
GAAAACAAGA GAGAGTACAT CGACCTCGTT ATCCAGTGGA GGTTCGTGAA CAGAGTCCAG 
AAGCAGATGA ATGCCTTCCT GGAGGGATTC ACGGAACTGC TCCCTATTGA TTTGATTAAA 
ATCTTTGATG AAAATGAACT GGAGTTGCTG ATGTGCGGCC TTGGCGATGT GGATGTGAAC 
GACTGGCGAC AACATTCTAT TTACAAGAAT GGCTACTGCC CGAACCACCC CGTGATCCAG 
TGGTTCTGGA AGGCTGTGCT GCTGATGGAC GCCGAGAGGC GCATCCGGTT ACTGCAGTTT 
GTCACAGGGA CGTCCCGAGT ACCTATGAAT GGATTTGCCG AGCTTTATGG TTCCAATGGT 
CCTCAGCTGT TTACAATAGA GCAATGGGGA AGTCCTGAAA AACTGCCCAG AGCTCACACG 
TGCTTTAATC GCCTTGACTT ACCTCCGTAT GAAACCTTCG AAGATTTACG AGAGAAGCTC 
CTCATGGCTG TGGAAAACGC CCAAGGCTTC GAGGGGGTGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073366.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073428.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu27440
Clone ID Related Accession (Same CDS sequence)	XM_006073366.1
Accession Version	XM_006073366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785536.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGGGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGCC ATCAGTGGCC 
TATGTACACA CCACGCCGGG CCTGCCCTCG GGCTGGGAAG AGAGGAAAGA TGCTAAGGGA 
CGCACATACT ATGTCAATCA TAACAATCGA ACCACAACTT GGACTCGGCC TATCGTGCAG 
CTCGCAGAAG ACGGCGCGTC CGGATCCGCC ACAAACAGTA ACAACCACCT AATCGAGCCT 
CAGATCCGCC GGCCTCGTAG CCTCAGCTCG CCAACAGTAA CTTTATCCGC CCCGCTGGAG 
GGCGCCAAGG ACTCACCCGT ACGTCGGGCT GTGAAAGATA CCCTTTCCAA TCCACAGTCC 
CCACAGCCAT CACCTTACAA CTCCCCCAAA CCACAACACA AAGTCACACA GAGCTTCCTG 
CCGCCCGGCT GGGAGATGAG GATAGCGCCC AACGGCCGGC CCTTCTTCAT TGACCACAAC 
ACAAAGACCA CGACGTGGGA AGATCCACGC CTGAAGTTTC CAGTACACAT GCGGTCAAAG 
GCATCCTTAA ACCCCAATGA CCTTGGCCCT CTTCCTCCTG GCTGGGAAGA AAGGATCCAC 
TTGGATGGCC GGACGTTTTA CATTGACCAC AATAGCAAAA TTACTCAGTG GGAAGACCCA 
AGACTGCAGA ACCCAGCTAT TACTGGTCCG GCTGTTCCTT ACTCCAGAGA ATTTAAGCAG 
AAATATGACT ACTTTAGAAA GAAATTAAAG AAACCTGCGG ATATTCCAAA TAGGTTTGAA 
ATGAAACTTC ACAGAAATAA CATATTTGAA GAATCCTATC GGAGAATCAT GTCTGTGAAA 
AGACCAGATG TCCTGAAAGC TAGGCTGTGG ATTGAGTTTG AATCGGAGAA AGGTCTGGAC 
TACGGGGGCG TGGCCAGAGA GTGGTTCTTC CTACTGTCCA AGGAGATGTT CAACCCTTAC 
TACGGCCTCT TCGAGTACTC GGCAACGGAC AACTACACAC TTCAGATCAA CCCCAATTCA 
GGCCTCTGTA ATGAGGACCA TCTGTCCTAT TTCACCTTCA TTGGAAGAGT TGCCGGGCTG 
GCAGTATTTC ATGGGAAACT TCTAGATGGT TTCTTCATTC GACCGTTTTA TAAAATGATG 
CTGGGAAAGC AGATAACTCT GAATGACATG GAATCTGTGG ATAGCGAATA TTACAACTCT 
TTGAAATGGA TCTTAGAAAA TGACCCAACT GAACTGGACC TTATGTTCTG CATTGATGAA 
GAGAACTTTG GGCAGACCTA TCAAGTGGAT CTGAAGCCCA ACGGGTCAGA AATAATGGTA 
ACAAATGAAA ACAAGAGAGA GTACATCGAC CTCGTTATCC AGTGGAGGTT CGTGAACAGA 
GTCCAGAAGC AGATGAATGC CTTCCTGGAG GGATTCACGG AACTGCTCCC TATTGATTTG 
ATTAAAATCT TTGATGAAAA TGAACTGGAG TTGCTGATGT GCGGCCTTGG CGATGTGGAT 
GTGAACGACT GGCGACAACA TTCTATTTAC AAGAATGGCT ACTGCCCGAA CCACCCCGTG 
ATCCAGTGGT TCTGGAAGGC TGTGCTGCTG ATGGACGCCG AGAGGCGCAT CCGGTTACTG 
CAGTTTGTCA CAGGGACGTC CCGAGTACCT ATGAATGGAT TTGCCGAGCT TTATGGTTCC 
AATGGTCCTC AGCTGTTTAC AATAGAGCAA TGGGGAAGTC CTGAAAAACT GCCCAGAGCT 
CACACGTGCT TTAATCGCCT TGACTTACCT CCGTATGAAA CCTTCGAAGA TTTACGAGAG 
AAGCTCCTCA TGGCTGTGGA AAACGCCCAA GGCTTCGAGG GGGTGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073428.1
CDS	66..3176
Translation	MPSGAEGQAGPAAAAPEPRRWSLQHVPDASGSSGKRCFVFQLQQPPASGGGPAPSGDFNFGFTGTKGDRLVRYPRIRLERSSSYPTQPRAELGSPPEERGHPDPEAPLRGRRVAPDAHRTEAVAERSPQGQGCTFKIRQDQNAGQQHFRILVTRGPEEAPQSPEEDAAAAGPASTGAGAPTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIVQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073366.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073366.1

ORF Insert Sequence:

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGGGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGCC ATCAGTGGCC 
TATGTACACA CCACGCCGGG CCTGCCCTCG GGCTGGGAAG AGAGGAAAGA TGCTAAGGGA 
CGCACATACT ATGTCAATCA TAACAATCGA ACCACAACTT GGACTCGGCC TATCGTGCAG 
CTCGCAGAAG ACGGCGCGTC CGGATCCGCC ACAAACAGTA ACAACCACCT AATCGAGCCT 
CAGATCCGCC GGCCTCGTAG CCTCAGCTCG CCAACAGTAA CTTTATCCGC CCCGCTGGAG 
GGCGCCAAGG ACTCACCCGT ACGTCGGGCT GTGAAAGATA CCCTTTCCAA TCCACAGTCC 
CCACAGCCAT CACCTTACAA CTCCCCCAAA CCACAACACA AAGTCACACA GAGCTTCCTG 
CCGCCCGGCT GGGAGATGAG GATAGCGCCC AACGGCCGGC CCTTCTTCAT TGACCACAAC 
ACAAAGACCA CGACGTGGGA AGATCCACGC CTGAAGTTTC CAGTACACAT GCGGTCAAAG 
GCATCCTTAA ACCCCAATGA CCTTGGCCCT CTTCCTCCTG GCTGGGAAGA AAGGATCCAC 
TTGGATGGCC GGACGTTTTA CATTGACCAC AATAGCAAAA TTACTCAGTG GGAAGACCCA 
AGACTGCAGA ACCCAGCTAT TACTGGTCCG GCTGTTCCTT ACTCCAGAGA ATTTAAGCAG 
AAATATGACT ACTTTAGAAA GAAATTAAAG AAACCTGCGG ATATTCCAAA TAGGTTTGAA 
ATGAAACTTC ACAGAAATAA CATATTTGAA GAATCCTATC GGAGAATCAT GTCTGTGAAA 
AGACCAGATG TCCTGAAAGC TAGGCTGTGG ATTGAGTTTG AATCGGAGAA AGGTCTGGAC 
TACGGGGGCG TGGCCAGAGA GTGGTTCTTC CTACTGTCCA AGGAGATGTT CAACCCTTAC 
TACGGCCTCT TCGAGTACTC GGCAACGGAC AACTACACAC TTCAGATCAA CCCCAATTCA 
GGCCTCTGTA ATGAGGACCA TCTGTCCTAT TTCACCTTCA TTGGAAGAGT TGCCGGGCTG 
GCAGTATTTC ATGGGAAACT TCTAGATGGT TTCTTCATTC GACCGTTTTA TAAAATGATG 
CTGGGAAAGC AGATAACTCT GAATGACATG GAATCTGTGG ATAGCGAATA TTACAACTCT 
TTGAAATGGA TCTTAGAAAA TGACCCAACT GAACTGGACC TTATGTTCTG CATTGATGAA 
GAGAACTTTG GGCAGACCTA TCAAGTGGAT CTGAAGCCCA ACGGGTCAGA AATAATGGTA 
ACAAATGAAA ACAAGAGAGA GTACATCGAC CTCGTTATCC AGTGGAGGTT CGTGAACAGA 
GTCCAGAAGC AGATGAATGC CTTCCTGGAG GGATTCACGG AACTGCTCCC TATTGATTTG 
ATTAAAATCT TTGATGAAAA TGAACTGGAG TTGCTGATGT GCGGCCTTGG CGATGTGGAT 
GTGAACGACT GGCGACAACA TTCTATTTAC AAGAATGGCT ACTGCCCGAA CCACCCCGTG 
ATCCAGTGGT TCTGGAAGGC TGTGCTGCTG ATGGACGCCG AGAGGCGCAT CCGGTTACTG 
CAGTTTGTCA CAGGGACGTC CCGAGTACCT ATGAATGGAT TTGCCGAGCT TTATGGTTCC 
AATGGTCCTC AGCTGTTTAC AATAGAGCAA TGGGGAAGTC CTGAAAAACT GCCCAGAGCT 
CACACGTGCT TTAATCGCCT TGACTTACCT CCGTATGAAA CCTTCGAAGA TTTACGAGAG 
AAGCTCCTCA TGGCTGTGGA AAACGCCCAA GGCTTCGAGG GGGTGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073368.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073430.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBu27442
Clone ID Related Accession (Same CDS sequence)	XM_006073368.1
Accession Version	XM_006073368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785536.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGGGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGGG CGCCAAGGAC 
TCACCCGTAC GTCGGGCTGT GAAAGATACC CTTTCCAATC CACAGTCCCC ACAGCCATCA 
CCTTACAACT CCCCCAAACC ACAACACAAA GTCACACAGA GCTTCCTGCC GCCCGGCTGG 
GAGATGAGGA TAGCGCCCAA CGGCCGGCCC TTCTTCATTG ACCACAACAC AAAGACCACG 
ACGTGGGAAG ATCCACGCCT GAAGTTTCCA GTACACATGC GGTCAAAGGC ATCCTTAAAC 
CCCAATGACC TTGGCCCTCT TCCTCCTGGC TGGGAAGAAA GGATCCACTT GGATGGCCGG 
ACGTTTTACA TTGACCACAG CAGCCAGGTG TTATGTGGCG AGGACGATGC CAGAGACTCA 
GTGCCCTCGT ACGATAGCAA AATTACTCAG TGGGAAGACC CAAGACTGCA GAACCCAGCT 
ATTACTGGTC CGGCTGTTCC TTACTCCAGA GAATTTAAGC AGAAATATGA CTACTTTAGA 
AAGAAATTAA AGAAACCTGC GGATATTCCA AATAGGTTTG AAATGAAACT TCACAGAAAT 
AACATATTTG AAGAATCCTA TCGGAGAATC ATGTCTGTGA AAAGACCAGA TGTCCTGAAA 
GCTAGGCTGT GGATTGAGTT TGAATCGGAG AAAGGTCTGG ACTACGGGGG CGTGGCCAGA 
GAGTGGTTCT TCCTACTGTC CAAGGAGATG TTCAACCCTT ACTACGGCCT CTTCGAGTAC 
TCGGCAACGG ACAACTACAC ACTTCAGATC AACCCCAATT CAGGCCTCTG TAATGAGGAC 
CATCTGTCCT ATTTCACCTT CATTGGAAGA GTTGCCGGGC TGGCAGTATT TCATGGGAAA 
CTTCTAGATG GTTTCTTCAT TCGACCGTTT TATAAAATGA TGCTGGGAAA GCAGATAACT 
CTGAATGACA TGGAATCTGT GGATAGCGAA TATTACAACT CTTTGAAATG GATCTTAGAA 
AATGACCCAA CTGAACTGGA CCTTATGTTC TGCATTGATG AAGAGAACTT TGGGCAGACC 
TATCAAGTGG ATCTGAAGCC CAACGGGTCA GAAATAATGG TAACAAATGA AAACAAGAGA 
GAGTACATCG ACCTCGTTAT CCAGTGGAGG TTCGTGAACA GAGTCCAGAA GCAGATGAAT 
GCCTTCCTGG AGGGATTCAC GGAACTGCTC CCTATTGATT TGATTAAAAT CTTTGATGAA 
AATGAACTGG AGTTGCTGAT GTGCGGCCTT GGCGATGTGG ATGTGAACGA CTGGCGACAA 
CATTCTATTT ACAAGAATGG CTACTGCCCG AACCACCCCG TGATCCAGTG GTTCTGGAAG 
GCTGTGCTGC TGATGGACGC CGAGAGGCGC ATCCGGTTAC TGCAGTTTGT CACAGGGACG 
TCCCGAGTAC CTATGAATGG ATTTGCCGAG CTTTATGGTT CCAATGGTCC TCAGCTGTTT 
ACAATAGAGC AATGGGGAAG TCCTGAAAAA CTGCCCAGAG CTCACACGTG CTTTAATCGC 
CTTGACTTAC CTCCGTATGA AACCTTCGAA GATTTACGAG AGAAGCTCCT CATGGCTGTG 
GAAAACGCCC AAGGCTTCGA GGGGGTGGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073430.1
CDS	66..2978
Translation	MPSGAEGQAGPAAAAPEPRRWSLQHVPDASGSSGKRCFVFQLQQPPASGGGPAPSGDFNFGFTGTKGDRLVRYPRIRLERSSSYPTQPRAELGSPPEERGHPDPEAPLRGRRVAPDAHRTEAVAERSPQGQGCTFKIRQDQNAGQQHFRILVTRGPEEAPQSPEEDAAAAGPASTGAGAPTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHSSQVLCGEDDARDSVPSYDSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073368.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073368.1

ORF Insert Sequence:

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGGGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGGG CGCCAAGGAC 
TCACCCGTAC GTCGGGCTGT GAAAGATACC CTTTCCAATC CACAGTCCCC ACAGCCATCA 
CCTTACAACT CCCCCAAACC ACAACACAAA GTCACACAGA GCTTCCTGCC GCCCGGCTGG 
GAGATGAGGA TAGCGCCCAA CGGCCGGCCC TTCTTCATTG ACCACAACAC AAAGACCACG 
ACGTGGGAAG ATCCACGCCT GAAGTTTCCA GTACACATGC GGTCAAAGGC ATCCTTAAAC 
CCCAATGACC TTGGCCCTCT TCCTCCTGGC TGGGAAGAAA GGATCCACTT GGATGGCCGG 
ACGTTTTACA TTGACCACAG CAGCCAGGTG TTATGTGGCG AGGACGATGC CAGAGACTCA 
GTGCCCTCGT ACGATAGCAA AATTACTCAG TGGGAAGACC CAAGACTGCA GAACCCAGCT 
ATTACTGGTC CGGCTGTTCC TTACTCCAGA GAATTTAAGC AGAAATATGA CTACTTTAGA 
AAGAAATTAA AGAAACCTGC GGATATTCCA AATAGGTTTG AAATGAAACT TCACAGAAAT 
AACATATTTG AAGAATCCTA TCGGAGAATC ATGTCTGTGA AAAGACCAGA TGTCCTGAAA 
GCTAGGCTGT GGATTGAGTT TGAATCGGAG AAAGGTCTGG ACTACGGGGG CGTGGCCAGA 
GAGTGGTTCT TCCTACTGTC CAAGGAGATG TTCAACCCTT ACTACGGCCT CTTCGAGTAC 
TCGGCAACGG ACAACTACAC ACTTCAGATC AACCCCAATT CAGGCCTCTG TAATGAGGAC 
CATCTGTCCT ATTTCACCTT CATTGGAAGA GTTGCCGGGC TGGCAGTATT TCATGGGAAA 
CTTCTAGATG GTTTCTTCAT TCGACCGTTT TATAAAATGA TGCTGGGAAA GCAGATAACT 
CTGAATGACA TGGAATCTGT GGATAGCGAA TATTACAACT CTTTGAAATG GATCTTAGAA 
AATGACCCAA CTGAACTGGA CCTTATGTTC TGCATTGATG AAGAGAACTT TGGGCAGACC 
TATCAAGTGG ATCTGAAGCC CAACGGGTCA GAAATAATGG TAACAAATGA AAACAAGAGA 
GAGTACATCG ACCTCGTTAT CCAGTGGAGG TTCGTGAACA GAGTCCAGAA GCAGATGAAT 
GCCTTCCTGG AGGGATTCAC GGAACTGCTC CCTATTGATT TGATTAAAAT CTTTGATGAA 
AATGAACTGG AGTTGCTGAT GTGCGGCCTT GGCGATGTGG ATGTGAACGA CTGGCGACAA 
CATTCTATTT ACAAGAATGG CTACTGCCCG AACCACCCCG TGATCCAGTG GTTCTGGAAG 
GCTGTGCTGC TGATGGACGC CGAGAGGCGC ATCCGGTTAC TGCAGTTTGT CACAGGGACG 
TCCCGAGTAC CTATGAATGG ATTTGCCGAG CTTTATGGTT CCAATGGTCC TCAGCTGTTT 
ACAATAGAGC AATGGGGAAG TCCTGAAAAA CTGCCCAGAG CTCACACGTG CTTTAATCGC 
CTTGACTTAC CTCCGTATGA AACCTTCGAA GATTTACGAG AGAAGCTCCT CATGGCTGTG 
GAAAACGCCC AAGGCTTCGA GGGGGTGGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4L ( XM_006073371.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006073433.1 Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBu27445
Clone ID Related Accession (Same CDS sequence)	XM_006073371.1
Accession Version	XM_006073371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase NEDD4-like isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785536.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGGGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGGA AGATCCACGC 
CTGAAGTTTC CAGTACACAT GCGGTCAAAG GCATCCTTAA ACCCCAATGA CCTTGGCCCT 
CTTCCTCCTG GCTGGGAAGA AAGGATCCAC TTGGATGGCC GGACGTTTTA CATTGACCAC 
AGCAGCCAGG TGTTATGTGG CGAGGACGAT GCCAGAGACT CAGTGCCCTC GTACGATAGC 
AAAATTACTC AGTGGGAAGA CCCAAGACTG CAGAACCCAG CTATTACTGG TCCGGCTGTT 
CCTTACTCCA GAGAATTTAA GCAGAAATAT GACTACTTTA GAAAGAAATT AAAGAAACCT 
GCGGATATTC CAAATAGGTT TGAAATGAAA CTTCACAGAA ATAACATATT TGAAGAATCC 
TATCGGAGAA TCATGTCTGT GAAAAGACCA GATGTCCTGA AAGCTAGGCT GTGGATTGAG 
TTTGAATCGG AGAAAGGTCT GGACTACGGG GGCGTGGCCA GAGAGTGGTT CTTCCTACTG 
TCCAAGGAGA TGTTCAACCC TTACTACGGC CTCTTCGAGT ACTCGGCAAC GGACAACTAC 
ACACTTCAGA TCAACCCCAA TTCAGGCCTC TGTAATGAGG ACCATCTGTC CTATTTCACC 
TTCATTGGAA GAGTTGCCGG GCTGGCAGTA TTTCATGGGA AACTTCTAGA TGGTTTCTTC 
ATTCGACCGT TTTATAAAAT GATGCTGGGA AAGCAGATAA CTCTGAATGA CATGGAATCT 
GTGGATAGCG AATATTACAA CTCTTTGAAA TGGATCTTAG AAAATGACCC AACTGAACTG 
GACCTTATGT TCTGCATTGA TGAAGAGAAC TTTGGGCAGA CCTATCAAGT GGATCTGAAG 
CCCAACGGGT CAGAAATAAT GGTAACAAAT GAAAACAAGA GAGAGTACAT CGACCTCGTT 
ATCCAGTGGA GGTTCGTGAA CAGAGTCCAG AAGCAGATGA ATGCCTTCCT GGAGGGATTC 
ACGGAACTGC TCCCTATTGA TTTGATTAAA ATCTTTGATG AAAATGAACT GGAGTTGCTG 
ATGTGCGGCC TTGGCGATGT GGATGTGAAC GACTGGCGAC AACATTCTAT TTACAAGAAT 
GGCTACTGCC CGAACCACCC CGTGATCCAG TGGTTCTGGA AGGCTGTGCT GCTGATGGAC 
GCCGAGAGGC GCATCCGGTT ACTGCAGTTT GTCACAGGGA CGTCCCGAGT ACCTATGAAT 
GGATTTGCCG AGCTTTATGG TTCCAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGA 
AGTCCTGAAA AACTGCCCAG AGCTCACACG TGCTTTAATC GCCTTGACTT ACCTCCGTAT 
GAAACCTTCG AAGATTTACG AGAGAAGCTC CTCATGGCTG TGGAAAACGC CCAAGGCTTC 
GAGGGGGTGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006073433.1
CDS	67..2781
Translation	MPSGAEGQAGPAAAAPEPRRWSLQHVPDASGSSGKRCFVFQLQQPPASGGGPAPSGDFNFGFTGTKGDRLVRYPRIRLERSSSYPTQPRAELGSPPEERGHPDPEAPLRGRRVAPDAHRTEAVAERSPQGQGCTFKIRQDQNAGQQHFRILVTRGPEEAPQSPEEDAAAAGPASTGAGAPTRDDFLGQVDIPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSEQRDEMEPGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDSNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGEGPEPWETISEEVNMSGDSLSLALPPPPASPVSRTSPQELSEELSRRLQVTPDSNGEQFGSLIQREPSSRLRSCSVTDAVAEQAHLPPEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHSSQVLCGEDDARDSVPSYDSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAERRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD

Target ORF information:

RefSeq Version	XM_006073371.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006073371.1

ORF Insert Sequence:

ATGCCCTCGG GAGCCGAGGG CCAGGCTGGG CCCGCAGCGG CGGCGCCCGA GCCCAGGCGC 
TGGTCCCTGC AGCATGTTCC AGATGCTTCT GGAAGCTCCG GGAAGCGCTG CTTTGTCTTC 
CAGCTGCAGC AGCCGCCAGC CAGTGGTGGC GGGCCTGCGC CGAGCGGCGA CTTCAACTTC 
GGCTTCACCG GCACCAAGGG GGACAGGTTG GTGAGGTATC CCCGAATCCG GCTGGAGAGG 
AGCAGCTCGT ACCCCACGCA GCCCCGGGCT GAGCTCGGGA GTCCCCCGGA GGAGCGCGGC 
CACCCAGACC CGGAGGCGCC CCTGCGGGGC CGCAGGGTGG CTCCCGATGC CCACAGGACC 
GAGGCGGTGG CCGAGAGGTC ACCACAGGGC CAGGGCTGCA CGTTTAAGAT CAGGCAGGAT 
CAAAACGCGG GGCAGCAGCA CTTCAGAATC CTCGTGACTC GGGGGCCCGA GGAAGCCCCC 
CAGAGTCCCG AGGAGGACGC CGCAGCGGCC GGCCCGGCTT CCACGGGAGC CGGCGCCCCG 
ACACGGGACG ACTTCCTGGG ACAGGTGGAC ATACCCCTTA GTCATCTCCC GACAGAAGAC 
CCAACCATGG AGCGACCCTA CACATTTAAG GACTTTCTCC TCCGGCCAAG AAGTCATAAA 
TCTCGGGTTA AGGGATTTTT GCGATTGAAA ATGGCCTACA TGCCCAAAAA CGGGGGCCAA 
GATGAAGAGA ACAGTGAGCA GCGGGATGAG ATGGAGCCCG GGTGGGAAGT CGTTGACTCC 
AATGACTCGG CTTCTCAGCA CCAGGAGGAG CTGCCCCCGC CTCCGCTGCC CCCCGGGTGG 
GAAGAAAAGG TGGACAACCT GGGCCGCACG TACTACGTGA ACCACAACAA CCGGACCACC 
CAGTGGCACC GGCCCAGCCT CATGGACGTG TCGTCCGAGT CCGACAGCAA CATCCGGCAG 
ATCAACCAGG AGGCCGCGCA TCGCCGCTTC CGCTCGCGCA GACATATCAG CGAGGACCTG 
GAGCCGGAGC CCAGCGAGGG AGGGGAGGGC CCCGAGCCTT GGGAGACCAT TTCAGAGGAA 
GTGAACATGA GCGGAGACTC GCTCAGCCTG GCGCTGCCCC CGCCACCAGC CTCGCCCGTG 
TCCCGGACGA GCCCCCAGGA GCTGTCCGAG GAGCTGAGCA GAAGGCTGCA GGTCACTCCC 
GACTCCAACG GCGAGCAGTT TGGGTCTTTG ATTCAGCGAG AGCCGTCCTC CCGGCTGAGG 
TCCTGCAGTG TCACTGATGC AGTTGCGGAG CAGGCTCATC TCCCGCCGGA AGATCCACGC 
CTGAAGTTTC CAGTACACAT GCGGTCAAAG GCATCCTTAA ACCCCAATGA CCTTGGCCCT 
CTTCCTCCTG GCTGGGAAGA AAGGATCCAC TTGGATGGCC GGACGTTTTA CATTGACCAC 
AGCAGCCAGG TGTTATGTGG CGAGGACGAT GCCAGAGACT CAGTGCCCTC GTACGATAGC 
AAAATTACTC AGTGGGAAGA CCCAAGACTG CAGAACCCAG CTATTACTGG TCCGGCTGTT 
CCTTACTCCA GAGAATTTAA GCAGAAATAT GACTACTTTA GAAAGAAATT AAAGAAACCT 
GCGGATATTC CAAATAGGTT TGAAATGAAA CTTCACAGAA ATAACATATT TGAAGAATCC 
TATCGGAGAA TCATGTCTGT GAAAAGACCA GATGTCCTGA AAGCTAGGCT GTGGATTGAG 
TTTGAATCGG AGAAAGGTCT GGACTACGGG GGCGTGGCCA GAGAGTGGTT CTTCCTACTG 
TCCAAGGAGA TGTTCAACCC TTACTACGGC CTCTTCGAGT ACTCGGCAAC GGACAACTAC 
ACACTTCAGA TCAACCCCAA TTCAGGCCTC TGTAATGAGG ACCATCTGTC CTATTTCACC 
TTCATTGGAA GAGTTGCCGG GCTGGCAGTA TTTCATGGGA AACTTCTAGA TGGTTTCTTC 
ATTCGACCGT TTTATAAAAT GATGCTGGGA AAGCAGATAA CTCTGAATGA CATGGAATCT 
GTGGATAGCG AATATTACAA CTCTTTGAAA TGGATCTTAG AAAATGACCC AACTGAACTG 
GACCTTATGT TCTGCATTGA TGAAGAGAAC TTTGGGCAGA CCTATCAAGT GGATCTGAAG 
CCCAACGGGT CAGAAATAAT GGTAACAAAT GAAAACAAGA GAGAGTACAT CGACCTCGTT 
ATCCAGTGGA GGTTCGTGAA CAGAGTCCAG AAGCAGATGA ATGCCTTCCT GGAGGGATTC 
ACGGAACTGC TCCCTATTGA TTTGATTAAA ATCTTTGATG AAAATGAACT GGAGTTGCTG 
ATGTGCGGCC TTGGCGATGT GGATGTGAAC GACTGGCGAC AACATTCTAT TTACAAGAAT 
GGCTACTGCC CGAACCACCC CGTGATCCAG TGGTTCTGGA AGGCTGTGCT GCTGATGGAC 
GCCGAGAGGC GCATCCGGTT ACTGCAGTTT GTCACAGGGA CGTCCCGAGT ACCTATGAAT 
GGATTTGCCG AGCTTTATGG TTCCAATGGT CCTCAGCTGT TTACAATAGA GCAATGGGGA 
AGTCCTGAAA AACTGCCCAG AGCTCACACG TGCTTTAATC GCCTTGACTT ACCTCCGTAT 
GAAACCTTCG AAGATTTACG AGAGAAGCTC CTCATGGCTG TGGAAAACGC CCAAGGCTTC 
GAGGGGGTGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NEDD4L
Entrez Gene ID	102416190
Full Name	neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NEDD4L cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

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