PLEKHG1 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following PLEKHG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLEKHG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu43696 XM_006080212.2
Latest version!
Bubalus bubalis pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBu33138 XM_006080212.1
Latest version!
Bubalus bubalis pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBu33138 XM_006080213.1
Latest version!
Bubalus bubalis pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu43696
    Clone ID Related Accession (Same CDS sequence) XM_006080212.2
    Accession Version XM_006080212.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product LOW QUALITY PROTEIN: pleckstrin homology domain-containing family G member 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037554.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006080212.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGCTGTC TGCGTAGCGC TCATCTCTGT TTATTCTGTT TTCTGCCCTT GCTCGTCTCA 
    TCTCCCACTT TCCCTGGGCC TCCTGAAGGA GGATCCGCAA CACAGGATGA GTGTGTGGGT
    GGAGGCCGAA GGCGGGTGAA CTGCCCGGAC CTGTCTGTCT TGGGTGTAGC TCCATCACAG
    GTGGACGGAA TCCTCCCAAC GGTGGCAGCC AGACAGGGCT GTCCACCCCG TCCCGGGCAC
    CCGGCAGGGC CGCGGCCACC TGGGGACATG CTGTCCACAA CCATGGTGGG GGCTCTGGTC
    AGAGGGTGGA GAAATCGAAG CTCTCCTCGA AGGGCAGAAT CCAAGCTTCT TATCCCTCAG
    AGCCAAGATG AAATTCAGAC TCGAGTTCTG CCGGGAGCAG AGTCAGATGA TAAAGTCAGC
    TCACCACCAG ATATTGAGGA CCTGCCGGGG GAAGTTTCAG ATGCGCACCA GTTGCGTCGT
    GAAGTACGGT CAGCTGTAAC ACAAGCTCCA TATCTCAGAA CCACAGCCAG GCACTCCTGC
    TCCGGGGACC TCGGGGCCAT GGAGCTCTCC GACAGTGACC GCCCGGTCAG CTTCGGCTCC
    ACGTCGTCCT CGGCTTCCTC CCGGGACAGC CATGGTTCCT TCGGCAGCAG AATGACCTTA
    GTTTCAAGCA GCCATCTGGG CTTGTTTCCT CAGGATAAGG AAGCAGGGGC CATAAAACTG
    GAGCTGATGC CAGCCAGGCC GTTTTCCAGC AGCGAGCTGC AGACTGACCG TGCGGCCGGG
    CAGCAGAGCG CAGACGAGGG TGGCGAGAGG CAGCCCCGGG CACAGCGCAG AGTGGAAGCC
    AACGGGGCGA CCAAAACGGG GGCCGAATCG GCCACCAGCC CCAAGCTGCT CTATGTGGAC
    AGAGTTGTGC AGGAAATTCT GGAGACCGAA CGGACTTACG TGCAAGATTT AAAGAGCATC
    GTAGAGGATT ACCTTGACTG TATCAGGGAC CAAACGAAAC TTCCTCTAGG GACAGATGAA
    AGATCAGCCC TTTTTGGAAA CATACAGGAT ATCTACCACT TTAATAGTGA ACTTCTGCAA
    GAGTTGGAAA ACTGTGAAAA TGATCCTGTG GCCATAGCAG AGTGCTTTGT ATCCAAGAGT
    GAAGAGTTCC ACATTTATAC CCAGTATTGC ACGAACTATC CAAGATCTGT GGCCGTGCTA
    ACAGAGTGTA TGAGGAACAA GATGCTGGCC AAATTCTTCA GGGAACGGCA GGAAACTTTG
    AAACATTCAC TGCCTCTGGG GTCCTATCTC TTGAAGCCAG TTCAGCGGAT TCTCAAGTAT
    CATCTCCTTT TGCATGAAAT AGAAAATCAC CTTGACAAGG ACACAGAGGG CTACGATGTG
    GTGCTTGATG CCATAGACAC GATGCAGAGA GTGGCCTGGC ACATCAACGA CATGAAGCGG
    AAGCATGAAC ACGCTGTCCG CCTGCAGGAG ATACAGAGTT TGCTCACCAA CTGGAAGGGG
    CCAGACCTGA CCAGCTACGG GGAGCTAGTG CTCGAAGGGA CCTTCCGCCT CCAGCGGGCC
    AAGAATGAGC GGACACTCTT CCTCTTTGAC AAGCTGCTGC TCATCACCAA GAAGAGAGAT
    GACACGTTTA CATACAAAGC CCACATCCTG TGCGGCAACC TCATGCTGGT AGAAGTGATC
    CCAAAGGAGC CCCTCAGCTT CAGCGTCTTC CACTACAAGA ACCCCAAGCT GCAGCACACG
    GTTCAGGCGA AGTCGCAGCA AGACAAGCGC CTGTGGGTTC TGCACCTGAA GAGGCTGATC
    CTGGAGAATC ATGCGGCAAA GATTCCAGCT AAGGCCAAGC AAGCAATACT TGAAATGGAT
    GCCATTCATT ACCCAGGCTT CTGTTACAGT CCCGAGGGAG AGACTTGTGG CTCTCGAAAT
    GGCTCTGCTC CGTATCGGCT GAGAAGGAAA TCTGAACCTT CCTCAAGATC ACATAAAGTT
    TTGAAGACCA GCGAAACAGC ACAAGACATT CAAAAGGTTT CCAGAGAGGA AGGGTCTCCC
    CAGTTGACTT CAGCAGGGCC CTCTCCTGCC CAGCGGAGCA GTCAGCCAAG CAGTTCAACC
    ATCCTGAGCA TGCTTCGTGG AGGCGGGGCT GTCAGAAACA TCTGGACCGA CCACCAGATC
    CGCCAAGCCC TGTTTCCCAG CCGAAGGTCG CAGGAGAACC AGGAGGATGA AGATGACTAT
    CAGATGTTCC TGCCATCCTT CTCCTCCTCG GATCTGAACT CAGCCCAGCT GTGTGAAGAT
    GGCACTTCTA GTCGACCTTG CAGTTGGCAC ATGGGACAAA TCGAGTCCTT GGAGACCTCC
    AGCTCTGGCC ACAGGATTGT GCGGCGGGCC AGCAGCGCTG GCGAGAGCAA CACATGTCCC
    CCTGACATAA GAACTAGGGA CAGTGGTGGT TCTCAGTACA GCTCCCGGAG GGAACTGCAG
    GGTGACCCTA AAACAGAGGG GCAGGACGGG ACAACTCCCT ACGGGTCCTC TATAGAGTTG
    ACTATAGATG ACATAGACCA TGTCTATGAC AACATAAGTT ACGAGGACTT GAAACTGATG
    GTTGCTAAAC GTGAAGATGC TGAAGTCAGT CCCCACAAAC CAGTCAGGGA CTCTGTTCGC
    CCCAAGAGTA CCCCCGAGCT GGCCTTCTCC AAGAAGCCAG CAGGACCTGA GGAGGAGAGC
    ACATTGCACC CCAGGAGAGA TGGAGCCCTC CCGGCCGGGG AGGCCTGGAA CCAAAGCGTG
    CACGACCTCC AGGTGATGGA GGAGAATGTC TATGACACCA TTGCGCTCCC AGAGGCGCCC
    TCCCGGGACT TCAAGGGCAG CAGCCCGAAG CGTCCTGTAA GGAGCACCTT CCTGGGCTTG
    AAGGCCGACT TGGGGTGCTG TGATAGTCTG CGGGCCTCTG CTTCCCAGGA CAGCCTCCAG
    TTCAGTGAGG ACGAGGCGCC CTACCCGCCA GGCCCCTCCG ATAACGATTA TCTGAGTTTG
    CTCTATAACT CCTTCGGCTG CAACTTGGCC CTAGCAGATC GGTCCATCTC TGATAAACTG
    TCTGAAGAAG TAGATGAAAT CTGGAATGAC TTGGAAAATT ACATCAAGAA AAACGAAGTG
    AAAGCCAGAG ACCGGCTCCT GGCCGCGTTT CCGGTTAGCA AAGATGACGT GCAGGAGCGG
    CCGCTGGCGG GCAGCGCCCC GGAGCTGAGC GCGCGCTCCC CGCTCTCGCT GCCCGTGGGC
    CGGGCTCTCC TTGCGCCGCC CGCAGACAGG CCCGGGCCAG CCCCCTGCGC CCTGGACCGA
    GCCCCAGCCG CCAAAGACAG CTCCTGCCTG AGTCTCCACC GGCTGTCGCT GGCCAGTGAC
    CTGCCGCCCA CAGAAAGTCC CTATGATGTG GCCGGCGGCA GCCTGTCCCC AGCGGACCTA
    GAGAACCCTG AGCCCGGGCT GGACAATGTA GACAAGACCA GGAGCAGGGT TTTCCTGATG
    GCCAGGCAGT ACAGTCAGAA GATCAAGAAG GCAAATCAGC TGCTAAAGGT GAAAAACCCA
    GAGCTGGAAC AGACGCTCAG TAGCCAGCCA CAGAAGCCCG CGCCCAAGGA CCTGGCGGCC
    ATCCTAGAAG AGAAGAGGCA GGGCGGGCCC GCCATCGGTG CCAGGATTGC TGAGTATTCC
    CAACTGTATG ACCAGATTGT ATTCAGAGAG ACCCCCTTTA AGACTCAGAA GGATGGCTGG
    GCCAGCCCTC AAGACCCCTC CAACCTCAGC TCTGCATCAC CTTCCCACGC CCAGCAAGGT
    AGCGAGAACT GGCTTTTGCA TTCAACCTAT AGTAACGGAG AGTTAGCTGA CTTCTGTCCC
    TGGCCAGAGC AAGACTTAAA GTCAAAATAT CCCATCTTCG AGATCAATAC AAGAAGTACC
    CCAAAACAAC TGTCTGCAGC TTGCTCCGTG CCTTCTCTTC AAACCTCCGA CCCTCTGCTG
    GGCTCCATGC AGAGACACAG CGTGGTGGTC AGCCAGCCAA ACAAAGAGAA CTCCTGCCAG
    GGCCATCTTT ACAACTCTCT GGGTCGGAAA GGAATCAGTG CTAAATCTCA GCCCTATAAC
    AGGTCCCAAT CATCTTCCTC CATCCTGATA AACAAGTCTG CAGACTCCAT CAACTACCCT
    AGTGAAGTGG CAAAGAAGCA GCCTGTCTCT CTACACAGAA GTTCAAGGTG GGAGAGTCAC
    CAGGACTTGC TCCCAAGCAT TGCTGACTCA GGTCAACAGG GCCTTGAAAA ACGCTCAGAT
    CTCACACTCC AGGACTCGCA GAAAGTTCTG GTAGTGAATA GAAATTTACC TTTAAGTGCT
    CAAATAGCTA CCCAGAACTA TTTTTCCAAT TTCAAAGAGA CTGAAGGAGA CGAAGATGAC
    TATGTGGAGA TCAAGTCAGA AGAAGATGAG TCTGAGTTAG AACTGTCTCA CAGTCGGAGG
    AGGAAATCTG ACCCAAAGAT TACAGACGCT GACTTTGCGG ATAACGTCTG CAGCCACACG
    ACTTCTTACT CTTTGAATAG TCCGTGCACT CCAAAAAAGC CAGTAAGTGG CAAACTTGGG
    ATTTCGCCGT ATCTGACGCC ATACAATGAT CCCAACCAAC TGAATGACTA TCTCTGGAAG
    GGGCCATCTC CCAATCAGCA AAATATTGTC CAGTCTCTAA GGGAAAAATT CCAGTGTCTT
    AGTTCAAGCA GCTTTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006080274.2
    CDS240..4940
    Translation

    Target ORF information:

    RefSeq Version XM_006080212.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006080212.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGCTGTC TGCGTAGCGC TCATCTCTGT TTATTCTGTT TTCTGCCCTT GCTCGTCTCA 
    TCTCCCACTT TCCCTGGGCC TCCTGAAGGA GGATCCGCAA CACAGGATGA GTGTGTGGGT
    GGAGGCCGAA GGCGGGTGAA CTGCCCGGAC CTGTCTGTCT TGGGTGTAGC TCCATCACAG
    GTGGACGGAA TCCTCCCAAC GGTGGCAGCC AGACAGGGCT GTCCACCCCG TCCCGGGCAC
    CCGGCAGGGC CGCGGCCACC TGGGGACATG CTGTCCACAA CCATGGTGGG GGCTCTGGTC
    AGAGGGTGGA GAAATCGAAG CTCTCCTCGA AGGGCAGAAT CCAAGCTTCT TATCCCTCAG
    AGCCAAGATG AAATTCAGAC TCGAGTTCTG CCGGGAGCAG AGTCAGATGA TAAAGTCAGC
    TCACCACCAG ATATTGAGGA CCTGCCGGGG GAAGTTTCAG ATGCGCACCA GTTGCGTCGT
    GAAGTACGGT CAGCTGTAAC ACAAGCTCCA TATCTCAGAA CCACAGCCAG GCACTCCTGC
    TCCGGGGACC TCGGGGCCAT GGAGCTCTCC GACAGTGACC GCCCGGTCAG CTTCGGCTCC
    ACGTCGTCCT CGGCTTCCTC CCGGGACAGC CATGGTTCCT TCGGCAGCAG AATGACCTTA
    GTTTCAAGCA GCCATCTGGG CTTGTTTCCT CAGGATAAGG AAGCAGGGGC CATAAAACTG
    GAGCTGATGC CAGCCAGGCC GTTTTCCAGC AGCGAGCTGC AGACTGACCG TGCGGCCGGG
    CAGCAGAGCG CAGACGAGGG TGGCGAGAGG CAGCCCCGGG CACAGCGCAG AGTGGAAGCC
    AACGGGGCGA CCAAAACGGG GGCCGAATCG GCCACCAGCC CCAAGCTGCT CTATGTGGAC
    AGAGTTGTGC AGGAAATTCT GGAGACCGAA CGGACTTACG TGCAAGATTT AAAGAGCATC
    GTAGAGGATT ACCTTGACTG TATCAGGGAC CAAACGAAAC TTCCTCTAGG GACAGATGAA
    AGATCAGCCC TTTTTGGAAA CATACAGGAT ATCTACCACT TTAATAGTGA ACTTCTGCAA
    GAGTTGGAAA ACTGTGAAAA TGATCCTGTG GCCATAGCAG AGTGCTTTGT ATCCAAGAGT
    GAAGAGTTCC ACATTTATAC CCAGTATTGC ACGAACTATC CAAGATCTGT GGCCGTGCTA
    ACAGAGTGTA TGAGGAACAA GATGCTGGCC AAATTCTTCA GGGAACGGCA GGAAACTTTG
    AAACATTCAC TGCCTCTGGG GTCCTATCTC TTGAAGCCAG TTCAGCGGAT TCTCAAGTAT
    CATCTCCTTT TGCATGAAAT AGAAAATCAC CTTGACAAGG ACACAGAGGG CTACGATGTG
    GTGCTTGATG CCATAGACAC GATGCAGAGA GTGGCCTGGC ACATCAACGA CATGAAGCGG
    AAGCATGAAC ACGCTGTCCG CCTGCAGGAG ATACAGAGTT TGCTCACCAA CTGGAAGGGG
    CCAGACCTGA CCAGCTACGG GGAGCTAGTG CTCGAAGGGA CCTTCCGCCT CCAGCGGGCC
    AAGAATGAGC GGACACTCTT CCTCTTTGAC AAGCTGCTGC TCATCACCAA GAAGAGAGAT
    GACACGTTTA CATACAAAGC CCACATCCTG TGCGGCAACC TCATGCTGGT AGAAGTGATC
    CCAAAGGAGC CCCTCAGCTT CAGCGTCTTC CACTACAAGA ACCCCAAGCT GCAGCACACG
    GTTCAGGCGA AGTCGCAGCA AGACAAGCGC CTGTGGGTTC TGCACCTGAA GAGGCTGATC
    CTGGAGAATC ATGCGGCAAA GATTCCAGCT AAGGCCAAGC AAGCAATACT TGAAATGGAT
    GCCATTCATT ACCCAGGCTT CTGTTACAGT CCCGAGGGAG AGACTTGTGG CTCTCGAAAT
    GGCTCTGCTC CGTATCGGCT GAGAAGGAAA TCTGAACCTT CCTCAAGATC ACATAAAGTT
    TTGAAGACCA GCGAAACAGC ACAAGACATT CAAAAGGTTT CCAGAGAGGA AGGGTCTCCC
    CAGTTGACTT CAGCAGGGCC CTCTCCTGCC CAGCGGAGCA GTCAGCCAAG CAGTTCAACC
    ATCCTGAGCA TGCTTCGTGG AGGCGGGGCT GTCAGAAACA TCTGGACCGA CCACCAGATC
    CGCCAAGCCC TGTTTCCCAG CCGAAGGTCG CAGGAGAACC AGGAGGATGA AGATGACTAT
    CAGATGTTCC TGCCATCCTT CTCCTCCTCG GATCTGAACT CAGCCCAGCT GTGTGAAGAT
    GGCACTTCTA GTCGACCTTG CAGTTGGCAC ATGGGACAAA TCGAGTCCTT GGAGACCTCC
    AGCTCTGGCC ACAGGATTGT GCGGCGGGCC AGCAGCGCTG GCGAGAGCAA CACATGTCCC
    CCTGACATAA GAACTAGGGA CAGTGGTGGT TCTCAGTACA GCTCCCGGAG GGAACTGCAG
    GGTGACCCTA AAACAGAGGG GCAGGACGGG ACAACTCCCT ACGGGTCCTC TATAGAGTTG
    ACTATAGATG ACATAGACCA TGTCTATGAC AACATAAGTT ACGAGGACTT GAAACTGATG
    GTTGCTAAAC GTGAAGATGC TGAAGTCAGT CCCCACAAAC CAGTCAGGGA CTCTGTTCGC
    CCCAAGAGTA CCCCCGAGCT GGCCTTCTCC AAGAAGCCAG CAGGACCTGA GGAGGAGAGC
    ACATTGCACC CCAGGAGAGA TGGAGCCCTC CCGGCCGGGG AGGCCTGGAA CCAAAGCGTG
    CACGACCTCC AGGTGATGGA GGAGAATGTC TATGACACCA TTGCGCTCCC AGAGGCGCCC
    TCCCGGGACT TCAAGGGCAG CAGCCCGAAG CGTCCTGTAA GGAGCACCTT CCTGGGCTTG
    AAGGCCGACT TGGGGTGCTG TGATAGTCTG CGGGCCTCTG CTTCCCAGGA CAGCCTCCAG
    TTCAGTGAGG ACGAGGCGCC CTACCCGCCA GGCCCCTCCG ATAACGATTA TCTGAGTTTG
    CTCTATAACT CCTTCGGCTG CAACTTGGCC CTAGCAGATC GGTCCATCTC TGATAAACTG
    TCTGAAGAAG TAGATGAAAT CTGGAATGAC TTGGAAAATT ACATCAAGAA AAACGAAGTG
    AAAGCCAGAG ACCGGCTCCT GGCCGCGTTT CCGGTTAGCA AAGATGACGT GCAGGAGCGG
    CCGCTGGCGG GCAGCGCCCC GGAGCTGAGC GCGCGCTCCC CGCTCTCGCT GCCCGTGGGC
    CGGGCTCTCC TTGCGCCGCC CGCAGACAGG CCCGGGCCAG CCCCCTGCGC CCTGGACCGA
    GCCCCAGCCG CCAAAGACAG CTCCTGCCTG AGTCTCCACC GGCTGTCGCT GGCCAGTGAC
    CTGCCGCCCA CAGAAAGTCC CTATGATGTG GCCGGCGGCA GCCTGTCCCC AGCGGACCTA
    GAGAACCCTG AGCCCGGGCT GGACAATGTA GACAAGACCA GGAGCAGGGT TTTCCTGATG
    GCCAGGCAGT ACAGTCAGAA GATCAAGAAG GCAAATCAGC TGCTAAAGGT GAAAAACCCA
    GAGCTGGAAC AGACGCTCAG TAGCCAGCCA CAGAAGCCCG CGCCCAAGGA CCTGGCGGCC
    ATCCTAGAAG AGAAGAGGCA GGGCGGGCCC GCCATCGGTG CCAGGATTGC TGAGTATTCC
    CAACTGTATG ACCAGATTGT ATTCAGAGAG ACCCCCTTTA AGACTCAGAA GGATGGCTGG
    GCCAGCCCTC AAGACCCCTC CAACCTCAGC TCTGCATCAC CTTCCCACGC CCAGCAAGGT
    AGCGAGAACT GGCTTTTGCA TTCAACCTAT AGTAACGGAG AGTTAGCTGA CTTCTGTCCC
    TGGCCAGAGC AAGACTTAAA GTCAAAATAT CCCATCTTCG AGATCAATAC AAGAAGTACC
    CCAAAACAAC TGTCTGCAGC TTGCTCCGTG CCTTCTCTTC AAACCTCCGA CCCTCTGCTG
    GGCTCCATGC AGAGACACAG CGTGGTGGTC AGCCAGCCAA ACAAAGAGAA CTCCTGCCAG
    GGCCATCTTT ACAACTCTCT GGGTCGGAAA GGAATCAGTG CTAAATCTCA GCCCTATAAC
    AGGTCCCAAT CATCTTCCTC CATCCTGATA AACAAGTCTG CAGACTCCAT CAACTACCCT
    AGTGAAGTGG CAAAGAAGCA GCCTGTCTCT CTACACAGAA GTTCAAGGTG GGAGAGTCAC
    CAGGACTTGC TCCCAAGCAT TGCTGACTCA GGTCAACAGG GCCTTGAAAA ACGCTCAGAT
    CTCACACTCC AGGACTCGCA GAAAGTTCTG GTAGTGAATA GAAATTTACC TTTAAGTGCT
    CAAATAGCTA CCCAGAACTA TTTTTCCAAT TTCAAAGAGA CTGAAGGAGA CGAAGATGAC
    TATGTGGAGA TCAAGTCAGA AGAAGATGAG TCTGAGTTAG AACTGTCTCA CAGTCGGAGG
    AGGAAATCTG ACCCAAAGAT TACAGACGCT GACTTTGCGG ATAACGTCTG CAGCCACACG
    ACTTCTTACT CTTTGAATAG TCCGTGCACT CCAAAAAAGC CAGTAAGTGG CAAACTTGGG
    ATTTCGCCGT ATCTGACGCC ATACAATGAT CCCAACCAAC TGAATGACTA TCTCTGGAAG
    GGGCCATCTC CCAATCAGCA AAATATTGTC CAGTCTCTAA GGGAAAAATT CCAGTGTCTT
    AGTTCAAGCA GCTTTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu33138
    Clone ID Related Accession (Same CDS sequence) XM_006080212.1 , XM_006080213.1
    Accession Version XM_006080212.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product pleckstrin homology domain-containing family G member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785881.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CCGACAGTGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCC 
    TCCCGGGACA GCCATGGTTC CTTCGGCAGC AGAATGACCT TAGTTTCAAG CAGCCATCTG
    GGCTTGTTTC CTCAGGATAA GGAAGCAGGG GCCATAAAAC TGGAGCTGAT GCCAGCCAGG
    CCGTTTTCCA GCAGCGAGCT GCAGACTGAC CGTGCGGCCG GGCAGCAGAA CGCAGACGAG
    GGCGGCGAGA GGCAGCCCCG GGCACAGCGC AGAGTGGAAG CCAACGGGGC GACCAAAACG
    GGGGCCGAAT CGGCCACCAG CCCCAAGCTG CTCTATGTGG ACAGAGTTGT GCAGGAAATT
    CTGGAGACCG AACGGACTTA CGTGCAAGAT TTAAAGAGCA TCGTAGAGGA TTACCTTGAC
    TGTATCAGGG ACCAAACGAA ACTTCCTCTA GGGACAGATG AAAGATCAGC CCTTTTTGGA
    AACATACAGG ATATCTACCA CTTTAATAGT GAACTTCTGC AAGAGTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATAGC AGAGTGCTTT GTATCCAAGA GTGAAGAGTT CCACATTTAT
    ACCCAGTATT GCACGAACTA TCCAAGATCT GTGGCCGTGC TAACAGAGTG TATGAGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGG CAGGAAACTT TGAAACATTC ACTGCCTCTG
    GGGTCCTATC TCTTGAAGCC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAG GGCTACGATG TGGTGCTTGA TGCCATAGAC
    ACGATGCAGA GAGTGGCCTG GCACATCAAC GACATGAAGC GGAAGCATGA ACACGCTGTC
    CGCCTGCAGG AGATACAGAG TTTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAC
    GGGGAGCTAG TGCTCGAAGG GACCTTCCGC CTCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACC AAGAAGAGAG ATGACACGTT TACATACAAA
    GCCCACATCC TGTGCGGCAA CCTCATGCTG GTAGAAGTGA TCCCAAAGGA GCCCCTCAGC
    TTCAGCGTCT TCCACTACAA GAACCCCAAG CTGCAGCACA CGGTTCAGGC GAAGTCGCAG
    CAAGACAAGC GCCTGTGGGT TCTGCACCTG AAGAGGCTGA TCCTGGAGAA TCATGCGGCA
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTACCCAGGC
    TTCTGTTACA GTCCCGAGGG AGAGACTTGT GGCTCTCGAA ATGGCTCTGC TCCGTATCGG
    CTGAGAAGGA AATCTGAACC TTCCTCAAGA TCACATAAAG TTTTGAAGAC CAGCGAAACA
    GCACAAGACA TTCAAAAGGT TTCCAGAGAG GAAGGGTCTC CCCAGTTGAC TTCAGCAGGG
    CCCTCTCCTG CCCAGCGGAG CAGTCAGCCA AGCAGTTCAA CCATCCTGAG CATGCTTCGT
    GGAGGCGGGG CTGTCAGAAA CATCTGGACC GACCACCAGA TCCGCCAAGC CCTGTTTCCC
    AGCCGAAGGT CGCAGGAGAA CCAGGAGGAT GAAGATGACT ATCAGATGTT CCTGCCATCC
    TTCTCCTCCT CGGATCTGAA CTCAGCCCAG CTGTGTGAAG ATGGCACTTC TAGTCGACCT
    TGCAGTTGGC ACATGGGACA AATCGAGTCC TTGGAGACCT CCAGCTCTGG CCACAGGATT
    GTGCGGCGGG CCAGCAGCGC TGGCGAGAGC AACACATGTC CCCCTGACAT AAGAACTAGG
    GACAGTGGTG GTTCTCAGTA CAGCTCCCGG AGGGAACTGC AGGGTGACCC TAAAACAGAG
    GGGCAGGACG GGACAACTCC CTACGGGTCC TCTATAGAGT TGACTATAGA TGACATAGAC
    CATGTCTATG ACAACATAAG TTACGAGGAC TTGAAACTGA TGGTTGCTAA ACGTGAAGAT
    GCTGAAGTCA GTCCCCACAA ACCAGTCAGG GACTCTGTTC GCCCCAAGAG TACCCCCGAG
    CTGGCCTTCT CCAAGAAGCC AGCAGGACCT GAGGAGGAGA GCACATTGCA CCCCAGGAGA
    GATGGAGCCC TCCCGGCCGG GGAGGCCTGG AACCAAAGCG TGCACGACCT CCAGGTGATG
    GAGGAGAATG TCTATGACAC CATTGCGCTC CCAGAGGCGC CCTCCCGGGA CTTCAAGGGC
    AGCAGCCCGA AGCGTCCTGT AAGGAGCACC TTCCTGGGCT TGAAGGCCGA CTTGGGGTGC
    TGTGATAGTC TGCGGGCCTC TGCTTCCCAG GACAGCCTCC AGTTCAGTGA GGACGAGGCG
    CCCTACCCGC CAGGCCCCTC CGATAACGAT TATCTGAGTT TGCTCTATAA CTCCTTCGGC
    TGCAACTTGG CCCTAGCAGA TCGGTCCATC TCTGATAAAC TGTCTGAAGA AGTAGATGAA
    ATCTGGAATG ACTTGGAAAA TTACATCAAG AAAAACGAAG TGAAAGCCAG AGACCGGCTC
    CTGGCCGCGT TTCCGGTTAG CAAAGATGAC GTGCAGGAGC GGCCGCTGGC GGGCAGCGCC
    CCGGAGCTGA GCGCGCGCTC CCCGCTCTCG CTGCCCGTGG GCCGGGCTCT CCTTGCGCCG
    CCCGCAGACA GGCCCGGGCC AGCCCCCTGC GCCCTGGACC GAGCCCCAGC CGCCAAAGAC
    AGCTCCTGCC TGAGTCTCCA CCGGCTGTCG CTGGCCAGTG ACCTGCCGCC CACAGAAAGT
    CCCTATGATG TGGCCGGCGG CAGCCTGTCC CCAGCGGACC TAGAGAACCC TGAGCCCGGG
    CTGGACAATG TAGACAAGAC CAGGAGCAGG GTTTTCCTGA TGGCCAGGCA GTACAGTCAG
    AAGATCAAGA AGGCAAATCA GCTGCTAAAG GTGAAAAACC CAGAGCTGGA ACAGACGCTC
    AGTAGCCAGC CACAGAAGCC CGCGCCCAAG GACCTGGCGG CCATCCTAGA AGAGAAGAGG
    CAGGGCGGGC CCGCCATCGG TGCCAGGATT GCTGAGTATT CCCAACTGTA TGACCAGATT
    GTATTCAGAG AGACCCCCTT TAAGACTCAG AAGGATGGCT GGGCCAGCCC TCAAGACCCC
    TCCAACCTCA GCTCTGCATC ACCTTCCCAC GCCCAGCAAG GTAGCGAGAA CTGGCTTTTG
    CATTCAACCT ATAGTAACGG AGAGTTAGCT GACTTCTGTC CCTGGCCAGA GCAAGACTTA
    AAGTCAAAAT ATCCCATCTT CGAGATCAAT ACAAGAAGTA CCCCAAAACA ACTGTCTGCA
    GCTTGCTCCG TGCCTTCTCT TCAAACCTCC GACCCTCTGC TGGGCTCCAT GCAGAGACAC
    AGCGTGGTGG TCAGCCAGCC AAACAAAGAG AACTCCTGCC AGGGCCATCT TTACAACTCT
    CTGGGTCGGA AAGGAATCAG TGCTAAATCT CAGCCCTATA ACAGGTCCCA ATCATCTTCC
    TCCATCCTGA TAAACAAGTC TGCAGACTCC ATCAACTACC CTAGTGAAGT GGCAAAGAAG
    CAGCCTGTCT CTCTACACAG AAGTTCAAGG TGGGAGAGTC ACCAGGACTT GCTCCCAAGC
    ATTGCTGACT CAGGTCAACA GGGCCTTGAA AAACGCTCAG ATCTCACACT CCAGGACTCG
    CAGAAAGTTC TGGTAGTGAA TAGAAATTTA CCTTTAAGTG CTCAAATAGC TACCCAGAAC
    TATTTTTCCA ATTTCAAAGA GACTGAAGGA GACGAAGATG ACTATGTGGA GATCAAGTCA
    GAAGAAGATG AGTCTGAGTT AGAACTGTCT CACAGTCGGA GGAGGAAATC TGACCCAAAG
    ATTACAGACG CTGACTTTGC GGATAACGTC TGCAGCCACA CGACTTCTTA CTCTTTGAAT
    AGTCCGTGCA CTCCAAAAAA GCCAGTAAGT GGCAAACTTG GGATTTCGCC GTATCTGACG
    CCATACAATG ATCCCAACCA ACTGAATGAC TATCTCTGGA AGGGGCCATC TCCCAATCAG
    CAAAATATTG TCCAGTCTCT AAGGGAAAAA TTCCAGTGTC TTAGTTCAAG CAGCTTTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006080274.1
    CDS253..4395
    Translation

    Target ORF information:

    RefSeq Version XM_006080212.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006080212.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CCGACAGTGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCC 
    TCCCGGGACA GCCATGGTTC CTTCGGCAGC AGAATGACCT TAGTTTCAAG CAGCCATCTG
    GGCTTGTTTC CTCAGGATAA GGAAGCAGGG GCCATAAAAC TGGAGCTGAT GCCAGCCAGG
    CCGTTTTCCA GCAGCGAGCT GCAGACTGAC CGTGCGGCCG GGCAGCAGAA CGCAGACGAG
    GGCGGCGAGA GGCAGCCCCG GGCACAGCGC AGAGTGGAAG CCAACGGGGC GACCAAAACG
    GGGGCCGAAT CGGCCACCAG CCCCAAGCTG CTCTATGTGG ACAGAGTTGT GCAGGAAATT
    CTGGAGACCG AACGGACTTA CGTGCAAGAT TTAAAGAGCA TCGTAGAGGA TTACCTTGAC
    TGTATCAGGG ACCAAACGAA ACTTCCTCTA GGGACAGATG AAAGATCAGC CCTTTTTGGA
    AACATACAGG ATATCTACCA CTTTAATAGT GAACTTCTGC AAGAGTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATAGC AGAGTGCTTT GTATCCAAGA GTGAAGAGTT CCACATTTAT
    ACCCAGTATT GCACGAACTA TCCAAGATCT GTGGCCGTGC TAACAGAGTG TATGAGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGG CAGGAAACTT TGAAACATTC ACTGCCTCTG
    GGGTCCTATC TCTTGAAGCC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAG GGCTACGATG TGGTGCTTGA TGCCATAGAC
    ACGATGCAGA GAGTGGCCTG GCACATCAAC GACATGAAGC GGAAGCATGA ACACGCTGTC
    CGCCTGCAGG AGATACAGAG TTTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAC
    GGGGAGCTAG TGCTCGAAGG GACCTTCCGC CTCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACC AAGAAGAGAG ATGACACGTT TACATACAAA
    GCCCACATCC TGTGCGGCAA CCTCATGCTG GTAGAAGTGA TCCCAAAGGA GCCCCTCAGC
    TTCAGCGTCT TCCACTACAA GAACCCCAAG CTGCAGCACA CGGTTCAGGC GAAGTCGCAG
    CAAGACAAGC GCCTGTGGGT TCTGCACCTG AAGAGGCTGA TCCTGGAGAA TCATGCGGCA
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTACCCAGGC
    TTCTGTTACA GTCCCGAGGG AGAGACTTGT GGCTCTCGAA ATGGCTCTGC TCCGTATCGG
    CTGAGAAGGA AATCTGAACC TTCCTCAAGA TCACATAAAG TTTTGAAGAC CAGCGAAACA
    GCACAAGACA TTCAAAAGGT TTCCAGAGAG GAAGGGTCTC CCCAGTTGAC TTCAGCAGGG
    CCCTCTCCTG CCCAGCGGAG CAGTCAGCCA AGCAGTTCAA CCATCCTGAG CATGCTTCGT
    GGAGGCGGGG CTGTCAGAAA CATCTGGACC GACCACCAGA TCCGCCAAGC CCTGTTTCCC
    AGCCGAAGGT CGCAGGAGAA CCAGGAGGAT GAAGATGACT ATCAGATGTT CCTGCCATCC
    TTCTCCTCCT CGGATCTGAA CTCAGCCCAG CTGTGTGAAG ATGGCACTTC TAGTCGACCT
    TGCAGTTGGC ACATGGGACA AATCGAGTCC TTGGAGACCT CCAGCTCTGG CCACAGGATT
    GTGCGGCGGG CCAGCAGCGC TGGCGAGAGC AACACATGTC CCCCTGACAT AAGAACTAGG
    GACAGTGGTG GTTCTCAGTA CAGCTCCCGG AGGGAACTGC AGGGTGACCC TAAAACAGAG
    GGGCAGGACG GGACAACTCC CTACGGGTCC TCTATAGAGT TGACTATAGA TGACATAGAC
    CATGTCTATG ACAACATAAG TTACGAGGAC TTGAAACTGA TGGTTGCTAA ACGTGAAGAT
    GCTGAAGTCA GTCCCCACAA ACCAGTCAGG GACTCTGTTC GCCCCAAGAG TACCCCCGAG
    CTGGCCTTCT CCAAGAAGCC AGCAGGACCT GAGGAGGAGA GCACATTGCA CCCCAGGAGA
    GATGGAGCCC TCCCGGCCGG GGAGGCCTGG AACCAAAGCG TGCACGACCT CCAGGTGATG
    GAGGAGAATG TCTATGACAC CATTGCGCTC CCAGAGGCGC CCTCCCGGGA CTTCAAGGGC
    AGCAGCCCGA AGCGTCCTGT AAGGAGCACC TTCCTGGGCT TGAAGGCCGA CTTGGGGTGC
    TGTGATAGTC TGCGGGCCTC TGCTTCCCAG GACAGCCTCC AGTTCAGTGA GGACGAGGCG
    CCCTACCCGC CAGGCCCCTC CGATAACGAT TATCTGAGTT TGCTCTATAA CTCCTTCGGC
    TGCAACTTGG CCCTAGCAGA TCGGTCCATC TCTGATAAAC TGTCTGAAGA AGTAGATGAA
    ATCTGGAATG ACTTGGAAAA TTACATCAAG AAAAACGAAG TGAAAGCCAG AGACCGGCTC
    CTGGCCGCGT TTCCGGTTAG CAAAGATGAC GTGCAGGAGC GGCCGCTGGC GGGCAGCGCC
    CCGGAGCTGA GCGCGCGCTC CCCGCTCTCG CTGCCCGTGG GCCGGGCTCT CCTTGCGCCG
    CCCGCAGACA GGCCCGGGCC AGCCCCCTGC GCCCTGGACC GAGCCCCAGC CGCCAAAGAC
    AGCTCCTGCC TGAGTCTCCA CCGGCTGTCG CTGGCCAGTG ACCTGCCGCC CACAGAAAGT
    CCCTATGATG TGGCCGGCGG CAGCCTGTCC CCAGCGGACC TAGAGAACCC TGAGCCCGGG
    CTGGACAATG TAGACAAGAC CAGGAGCAGG GTTTTCCTGA TGGCCAGGCA GTACAGTCAG
    AAGATCAAGA AGGCAAATCA GCTGCTAAAG GTGAAAAACC CAGAGCTGGA ACAGACGCTC
    AGTAGCCAGC CACAGAAGCC CGCGCCCAAG GACCTGGCGG CCATCCTAGA AGAGAAGAGG
    CAGGGCGGGC CCGCCATCGG TGCCAGGATT GCTGAGTATT CCCAACTGTA TGACCAGATT
    GTATTCAGAG AGACCCCCTT TAAGACTCAG AAGGATGGCT GGGCCAGCCC TCAAGACCCC
    TCCAACCTCA GCTCTGCATC ACCTTCCCAC GCCCAGCAAG GTAGCGAGAA CTGGCTTTTG
    CATTCAACCT ATAGTAACGG AGAGTTAGCT GACTTCTGTC CCTGGCCAGA GCAAGACTTA
    AAGTCAAAAT ATCCCATCTT CGAGATCAAT ACAAGAAGTA CCCCAAAACA ACTGTCTGCA
    GCTTGCTCCG TGCCTTCTCT TCAAACCTCC GACCCTCTGC TGGGCTCCAT GCAGAGACAC
    AGCGTGGTGG TCAGCCAGCC AAACAAAGAG AACTCCTGCC AGGGCCATCT TTACAACTCT
    CTGGGTCGGA AAGGAATCAG TGCTAAATCT CAGCCCTATA ACAGGTCCCA ATCATCTTCC
    TCCATCCTGA TAAACAAGTC TGCAGACTCC ATCAACTACC CTAGTGAAGT GGCAAAGAAG
    CAGCCTGTCT CTCTACACAG AAGTTCAAGG TGGGAGAGTC ACCAGGACTT GCTCCCAAGC
    ATTGCTGACT CAGGTCAACA GGGCCTTGAA AAACGCTCAG ATCTCACACT CCAGGACTCG
    CAGAAAGTTC TGGTAGTGAA TAGAAATTTA CCTTTAAGTG CTCAAATAGC TACCCAGAAC
    TATTTTTCCA ATTTCAAAGA GACTGAAGGA GACGAAGATG ACTATGTGGA GATCAAGTCA
    GAAGAAGATG AGTCTGAGTT AGAACTGTCT CACAGTCGGA GGAGGAAATC TGACCCAAAG
    ATTACAGACG CTGACTTTGC GGATAACGTC TGCAGCCACA CGACTTCTTA CTCTTTGAAT
    AGTCCGTGCA CTCCAAAAAA GCCAGTAAGT GGCAAACTTG GGATTTCGCC GTATCTGACG
    CCATACAATG ATCCCAACCA ACTGAATGAC TATCTCTGGA AGGGGCCATC TCCCAATCAG
    CAAAATATTG TCCAGTCTCT AAGGGAAAAA TTCCAGTGTC TTAGTTCAAG CAGCTTTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu33138
    Clone ID Related Accession (Same CDS sequence) XM_006080212.1 , XM_006080213.1
    Accession Version XM_006080213.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product pleckstrin homology domain-containing family G member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785881.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CCGACAGTGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCC 
    TCCCGGGACA GCCATGGTTC CTTCGGCAGC AGAATGACCT TAGTTTCAAG CAGCCATCTG
    GGCTTGTTTC CTCAGGATAA GGAAGCAGGG GCCATAAAAC TGGAGCTGAT GCCAGCCAGG
    CCGTTTTCCA GCAGCGAGCT GCAGACTGAC CGTGCGGCCG GGCAGCAGAA CGCAGACGAG
    GGCGGCGAGA GGCAGCCCCG GGCACAGCGC AGAGTGGAAG CCAACGGGGC GACCAAAACG
    GGGGCCGAAT CGGCCACCAG CCCCAAGCTG CTCTATGTGG ACAGAGTTGT GCAGGAAATT
    CTGGAGACCG AACGGACTTA CGTGCAAGAT TTAAAGAGCA TCGTAGAGGA TTACCTTGAC
    TGTATCAGGG ACCAAACGAA ACTTCCTCTA GGGACAGATG AAAGATCAGC CCTTTTTGGA
    AACATACAGG ATATCTACCA CTTTAATAGT GAACTTCTGC AAGAGTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATAGC AGAGTGCTTT GTATCCAAGA GTGAAGAGTT CCACATTTAT
    ACCCAGTATT GCACGAACTA TCCAAGATCT GTGGCCGTGC TAACAGAGTG TATGAGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGG CAGGAAACTT TGAAACATTC ACTGCCTCTG
    GGGTCCTATC TCTTGAAGCC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAG GGCTACGATG TGGTGCTTGA TGCCATAGAC
    ACGATGCAGA GAGTGGCCTG GCACATCAAC GACATGAAGC GGAAGCATGA ACACGCTGTC
    CGCCTGCAGG AGATACAGAG TTTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAC
    GGGGAGCTAG TGCTCGAAGG GACCTTCCGC CTCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACC AAGAAGAGAG ATGACACGTT TACATACAAA
    GCCCACATCC TGTGCGGCAA CCTCATGCTG GTAGAAGTGA TCCCAAAGGA GCCCCTCAGC
    TTCAGCGTCT TCCACTACAA GAACCCCAAG CTGCAGCACA CGGTTCAGGC GAAGTCGCAG
    CAAGACAAGC GCCTGTGGGT TCTGCACCTG AAGAGGCTGA TCCTGGAGAA TCATGCGGCA
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTACCCAGGC
    TTCTGTTACA GTCCCGAGGG AGAGACTTGT GGCTCTCGAA ATGGCTCTGC TCCGTATCGG
    CTGAGAAGGA AATCTGAACC TTCCTCAAGA TCACATAAAG TTTTGAAGAC CAGCGAAACA
    GCACAAGACA TTCAAAAGGT TTCCAGAGAG GAAGGGTCTC CCCAGTTGAC TTCAGCAGGG
    CCCTCTCCTG CCCAGCGGAG CAGTCAGCCA AGCAGTTCAA CCATCCTGAG CATGCTTCGT
    GGAGGCGGGG CTGTCAGAAA CATCTGGACC GACCACCAGA TCCGCCAAGC CCTGTTTCCC
    AGCCGAAGGT CGCAGGAGAA CCAGGAGGAT GAAGATGACT ATCAGATGTT CCTGCCATCC
    TTCTCCTCCT CGGATCTGAA CTCAGCCCAG CTGTGTGAAG ATGGCACTTC TAGTCGACCT
    TGCAGTTGGC ACATGGGACA AATCGAGTCC TTGGAGACCT CCAGCTCTGG CCACAGGATT
    GTGCGGCGGG CCAGCAGCGC TGGCGAGAGC AACACATGTC CCCCTGACAT AAGAACTAGG
    GACAGTGGTG GTTCTCAGTA CAGCTCCCGG AGGGAACTGC AGGGTGACCC TAAAACAGAG
    GGGCAGGACG GGACAACTCC CTACGGGTCC TCTATAGAGT TGACTATAGA TGACATAGAC
    CATGTCTATG ACAACATAAG TTACGAGGAC TTGAAACTGA TGGTTGCTAA ACGTGAAGAT
    GCTGAAGTCA GTCCCCACAA ACCAGTCAGG GACTCTGTTC GCCCCAAGAG TACCCCCGAG
    CTGGCCTTCT CCAAGAAGCC AGCAGGACCT GAGGAGGAGA GCACATTGCA CCCCAGGAGA
    GATGGAGCCC TCCCGGCCGG GGAGGCCTGG AACCAAAGCG TGCACGACCT CCAGGTGATG
    GAGGAGAATG TCTATGACAC CATTGCGCTC CCAGAGGCGC CCTCCCGGGA CTTCAAGGGC
    AGCAGCCCGA AGCGTCCTGT AAGGAGCACC TTCCTGGGCT TGAAGGCCGA CTTGGGGTGC
    TGTGATAGTC TGCGGGCCTC TGCTTCCCAG GACAGCCTCC AGTTCAGTGA GGACGAGGCG
    CCCTACCCGC CAGGCCCCTC CGATAACGAT TATCTGAGTT TGCTCTATAA CTCCTTCGGC
    TGCAACTTGG CCCTAGCAGA TCGGTCCATC TCTGATAAAC TGTCTGAAGA AGTAGATGAA
    ATCTGGAATG ACTTGGAAAA TTACATCAAG AAAAACGAAG TGAAAGCCAG AGACCGGCTC
    CTGGCCGCGT TTCCGGTTAG CAAAGATGAC GTGCAGGAGC GGCCGCTGGC GGGCAGCGCC
    CCGGAGCTGA GCGCGCGCTC CCCGCTCTCG CTGCCCGTGG GCCGGGCTCT CCTTGCGCCG
    CCCGCAGACA GGCCCGGGCC AGCCCCCTGC GCCCTGGACC GAGCCCCAGC CGCCAAAGAC
    AGCTCCTGCC TGAGTCTCCA CCGGCTGTCG CTGGCCAGTG ACCTGCCGCC CACAGAAAGT
    CCCTATGATG TGGCCGGCGG CAGCCTGTCC CCAGCGGACC TAGAGAACCC TGAGCCCGGG
    CTGGACAATG TAGACAAGAC CAGGAGCAGG GTTTTCCTGA TGGCCAGGCA GTACAGTCAG
    AAGATCAAGA AGGCAAATCA GCTGCTAAAG GTGAAAAACC CAGAGCTGGA ACAGACGCTC
    AGTAGCCAGC CACAGAAGCC CGCGCCCAAG GACCTGGCGG CCATCCTAGA AGAGAAGAGG
    CAGGGCGGGC CCGCCATCGG TGCCAGGATT GCTGAGTATT CCCAACTGTA TGACCAGATT
    GTATTCAGAG AGACCCCCTT TAAGACTCAG AAGGATGGCT GGGCCAGCCC TCAAGACCCC
    TCCAACCTCA GCTCTGCATC ACCTTCCCAC GCCCAGCAAG GTAGCGAGAA CTGGCTTTTG
    CATTCAACCT ATAGTAACGG AGAGTTAGCT GACTTCTGTC CCTGGCCAGA GCAAGACTTA
    AAGTCAAAAT ATCCCATCTT CGAGATCAAT ACAAGAAGTA CCCCAAAACA ACTGTCTGCA
    GCTTGCTCCG TGCCTTCTCT TCAAACCTCC GACCCTCTGC TGGGCTCCAT GCAGAGACAC
    AGCGTGGTGG TCAGCCAGCC AAACAAAGAG AACTCCTGCC AGGGCCATCT TTACAACTCT
    CTGGGTCGGA AAGGAATCAG TGCTAAATCT CAGCCCTATA ACAGGTCCCA ATCATCTTCC
    TCCATCCTGA TAAACAAGTC TGCAGACTCC ATCAACTACC CTAGTGAAGT GGCAAAGAAG
    CAGCCTGTCT CTCTACACAG AAGTTCAAGG TGGGAGAGTC ACCAGGACTT GCTCCCAAGC
    ATTGCTGACT CAGGTCAACA GGGCCTTGAA AAACGCTCAG ATCTCACACT CCAGGACTCG
    CAGAAAGTTC TGGTAGTGAA TAGAAATTTA CCTTTAAGTG CTCAAATAGC TACCCAGAAC
    TATTTTTCCA ATTTCAAAGA GACTGAAGGA GACGAAGATG ACTATGTGGA GATCAAGTCA
    GAAGAAGATG AGTCTGAGTT AGAACTGTCT CACAGTCGGA GGAGGAAATC TGACCCAAAG
    ATTACAGACG CTGACTTTGC GGATAACGTC TGCAGCCACA CGACTTCTTA CTCTTTGAAT
    AGTCCGTGCA CTCCAAAAAA GCCAGTAAGT GGCAAACTTG GGATTTCGCC GTATCTGACG
    CCATACAATG ATCCCAACCA ACTGAATGAC TATCTCTGGA AGGGGCCATC TCCCAATCAG
    CAAAATATTG TCCAGTCTCT AAGGGAAAAA TTCCAGTGTC TTAGTTCAAG CAGCTTTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006080275.1
    CDS165..4307
    Translation

    Target ORF information:

    RefSeq Version XM_006080213.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis pleckstrin homology domain containing, family G (with RhoGef domain) member 1 (PLEKHG1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006080213.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAGCTCT CCGACAGTGA CCGCCCGGTC AGCTTCGGCT CCACGTCGTC CTCGGCTTCC 
    TCCCGGGACA GCCATGGTTC CTTCGGCAGC AGAATGACCT TAGTTTCAAG CAGCCATCTG
    GGCTTGTTTC CTCAGGATAA GGAAGCAGGG GCCATAAAAC TGGAGCTGAT GCCAGCCAGG
    CCGTTTTCCA GCAGCGAGCT GCAGACTGAC CGTGCGGCCG GGCAGCAGAA CGCAGACGAG
    GGCGGCGAGA GGCAGCCCCG GGCACAGCGC AGAGTGGAAG CCAACGGGGC GACCAAAACG
    GGGGCCGAAT CGGCCACCAG CCCCAAGCTG CTCTATGTGG ACAGAGTTGT GCAGGAAATT
    CTGGAGACCG AACGGACTTA CGTGCAAGAT TTAAAGAGCA TCGTAGAGGA TTACCTTGAC
    TGTATCAGGG ACCAAACGAA ACTTCCTCTA GGGACAGATG AAAGATCAGC CCTTTTTGGA
    AACATACAGG ATATCTACCA CTTTAATAGT GAACTTCTGC AAGAGTTGGA AAACTGTGAA
    AATGATCCTG TGGCCATAGC AGAGTGCTTT GTATCCAAGA GTGAAGAGTT CCACATTTAT
    ACCCAGTATT GCACGAACTA TCCAAGATCT GTGGCCGTGC TAACAGAGTG TATGAGGAAC
    AAGATGCTGG CCAAATTCTT CAGGGAACGG CAGGAAACTT TGAAACATTC ACTGCCTCTG
    GGGTCCTATC TCTTGAAGCC AGTTCAGCGG ATTCTCAAGT ATCATCTCCT TTTGCATGAA
    ATAGAAAATC ACCTTGACAA GGACACAGAG GGCTACGATG TGGTGCTTGA TGCCATAGAC
    ACGATGCAGA GAGTGGCCTG GCACATCAAC GACATGAAGC GGAAGCATGA ACACGCTGTC
    CGCCTGCAGG AGATACAGAG TTTGCTCACC AACTGGAAGG GGCCAGACCT GACCAGCTAC
    GGGGAGCTAG TGCTCGAAGG GACCTTCCGC CTCCAGCGGG CCAAGAATGA GCGGACACTC
    TTCCTCTTTG ACAAGCTGCT GCTCATCACC AAGAAGAGAG ATGACACGTT TACATACAAA
    GCCCACATCC TGTGCGGCAA CCTCATGCTG GTAGAAGTGA TCCCAAAGGA GCCCCTCAGC
    TTCAGCGTCT TCCACTACAA GAACCCCAAG CTGCAGCACA CGGTTCAGGC GAAGTCGCAG
    CAAGACAAGC GCCTGTGGGT TCTGCACCTG AAGAGGCTGA TCCTGGAGAA TCATGCGGCA
    AAGATTCCAG CTAAGGCCAA GCAAGCAATA CTTGAAATGG ATGCCATTCA TTACCCAGGC
    TTCTGTTACA GTCCCGAGGG AGAGACTTGT GGCTCTCGAA ATGGCTCTGC TCCGTATCGG
    CTGAGAAGGA AATCTGAACC TTCCTCAAGA TCACATAAAG TTTTGAAGAC CAGCGAAACA
    GCACAAGACA TTCAAAAGGT TTCCAGAGAG GAAGGGTCTC CCCAGTTGAC TTCAGCAGGG
    CCCTCTCCTG CCCAGCGGAG CAGTCAGCCA AGCAGTTCAA CCATCCTGAG CATGCTTCGT
    GGAGGCGGGG CTGTCAGAAA CATCTGGACC GACCACCAGA TCCGCCAAGC CCTGTTTCCC
    AGCCGAAGGT CGCAGGAGAA CCAGGAGGAT GAAGATGACT ATCAGATGTT CCTGCCATCC
    TTCTCCTCCT CGGATCTGAA CTCAGCCCAG CTGTGTGAAG ATGGCACTTC TAGTCGACCT
    TGCAGTTGGC ACATGGGACA AATCGAGTCC TTGGAGACCT CCAGCTCTGG CCACAGGATT
    GTGCGGCGGG CCAGCAGCGC TGGCGAGAGC AACACATGTC CCCCTGACAT AAGAACTAGG
    GACAGTGGTG GTTCTCAGTA CAGCTCCCGG AGGGAACTGC AGGGTGACCC TAAAACAGAG
    GGGCAGGACG GGACAACTCC CTACGGGTCC TCTATAGAGT TGACTATAGA TGACATAGAC
    CATGTCTATG ACAACATAAG TTACGAGGAC TTGAAACTGA TGGTTGCTAA ACGTGAAGAT
    GCTGAAGTCA GTCCCCACAA ACCAGTCAGG GACTCTGTTC GCCCCAAGAG TACCCCCGAG
    CTGGCCTTCT CCAAGAAGCC AGCAGGACCT GAGGAGGAGA GCACATTGCA CCCCAGGAGA
    GATGGAGCCC TCCCGGCCGG GGAGGCCTGG AACCAAAGCG TGCACGACCT CCAGGTGATG
    GAGGAGAATG TCTATGACAC CATTGCGCTC CCAGAGGCGC CCTCCCGGGA CTTCAAGGGC
    AGCAGCCCGA AGCGTCCTGT AAGGAGCACC TTCCTGGGCT TGAAGGCCGA CTTGGGGTGC
    TGTGATAGTC TGCGGGCCTC TGCTTCCCAG GACAGCCTCC AGTTCAGTGA GGACGAGGCG
    CCCTACCCGC CAGGCCCCTC CGATAACGAT TATCTGAGTT TGCTCTATAA CTCCTTCGGC
    TGCAACTTGG CCCTAGCAGA TCGGTCCATC TCTGATAAAC TGTCTGAAGA AGTAGATGAA
    ATCTGGAATG ACTTGGAAAA TTACATCAAG AAAAACGAAG TGAAAGCCAG AGACCGGCTC
    CTGGCCGCGT TTCCGGTTAG CAAAGATGAC GTGCAGGAGC GGCCGCTGGC GGGCAGCGCC
    CCGGAGCTGA GCGCGCGCTC CCCGCTCTCG CTGCCCGTGG GCCGGGCTCT CCTTGCGCCG
    CCCGCAGACA GGCCCGGGCC AGCCCCCTGC GCCCTGGACC GAGCCCCAGC CGCCAAAGAC
    AGCTCCTGCC TGAGTCTCCA CCGGCTGTCG CTGGCCAGTG ACCTGCCGCC CACAGAAAGT
    CCCTATGATG TGGCCGGCGG CAGCCTGTCC CCAGCGGACC TAGAGAACCC TGAGCCCGGG
    CTGGACAATG TAGACAAGAC CAGGAGCAGG GTTTTCCTGA TGGCCAGGCA GTACAGTCAG
    AAGATCAAGA AGGCAAATCA GCTGCTAAAG GTGAAAAACC CAGAGCTGGA ACAGACGCTC
    AGTAGCCAGC CACAGAAGCC CGCGCCCAAG GACCTGGCGG CCATCCTAGA AGAGAAGAGG
    CAGGGCGGGC CCGCCATCGG TGCCAGGATT GCTGAGTATT CCCAACTGTA TGACCAGATT
    GTATTCAGAG AGACCCCCTT TAAGACTCAG AAGGATGGCT GGGCCAGCCC TCAAGACCCC
    TCCAACCTCA GCTCTGCATC ACCTTCCCAC GCCCAGCAAG GTAGCGAGAA CTGGCTTTTG
    CATTCAACCT ATAGTAACGG AGAGTTAGCT GACTTCTGTC CCTGGCCAGA GCAAGACTTA
    AAGTCAAAAT ATCCCATCTT CGAGATCAAT ACAAGAAGTA CCCCAAAACA ACTGTCTGCA
    GCTTGCTCCG TGCCTTCTCT TCAAACCTCC GACCCTCTGC TGGGCTCCAT GCAGAGACAC
    AGCGTGGTGG TCAGCCAGCC AAACAAAGAG AACTCCTGCC AGGGCCATCT TTACAACTCT
    CTGGGTCGGA AAGGAATCAG TGCTAAATCT CAGCCCTATA ACAGGTCCCA ATCATCTTCC
    TCCATCCTGA TAAACAAGTC TGCAGACTCC ATCAACTACC CTAGTGAAGT GGCAAAGAAG
    CAGCCTGTCT CTCTACACAG AAGTTCAAGG TGGGAGAGTC ACCAGGACTT GCTCCCAAGC
    ATTGCTGACT CAGGTCAACA GGGCCTTGAA AAACGCTCAG ATCTCACACT CCAGGACTCG
    CAGAAAGTTC TGGTAGTGAA TAGAAATTTA CCTTTAAGTG CTCAAATAGC TACCCAGAAC
    TATTTTTCCA ATTTCAAAGA GACTGAAGGA GACGAAGATG ACTATGTGGA GATCAAGTCA
    GAAGAAGATG AGTCTGAGTT AGAACTGTCT CACAGTCGGA GGAGGAAATC TGACCCAAAG
    ATTACAGACG CTGACTTTGC GGATAACGTC TGCAGCCACA CGACTTCTTA CTCTTTGAAT
    AGTCCGTGCA CTCCAAAAAA GCCAGTAAGT GGCAAACTTG GGATTTCGCC GTATCTGACG
    CCATACAATG ATCCCAACCA ACTGAATGAC TATCTCTGGA AGGGGCCATC TCCCAATCAG
    CAAAATATTG TCCAGTCTCT AAGGGAAAAA TTCCAGTGTC TTAGTTCAAG CAGCTTTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.