NOTCH2 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following NOTCH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOTCH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu07607 XM_006049346.1
Latest version!
Bubalus bubalis notch 2 (NOTCH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBu07608 XM_006049347.1
Latest version!
Bubalus bubalis notch 2 (NOTCH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBu07609 XM_006049348.1
Latest version!
Bubalus bubalis notch 2 (NOTCH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBu68174 XM_006049346.2
Latest version!
Bubalus bubalis notch 2 (NOTCH2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu07607
    Clone ID Related Accession (Same CDS sequence) XM_006049346.1
    Accession Version XM_006049346.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product neurogenic locus notch homolog protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784196.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCCCGCCC TGCGCCCCGC TCCGCTGTGG GCGCTGCTGG CACTGTGGCT GTGCCGCGCG 
    GCCCCGGCGC GTGCATTGCA ATGTCGAGAT GGCTATGAGC CTTGTGTAAA TGAAGGAATA
    TGTGTTACCT ACCACAACGG TACAGGATAC TGCAAGTGTC CAGAGGGCTT CTTGGGAGAA
    TATTGTCAGC ATCGAGACCC CTGTGAGAAG AACCGTTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCTA TGTTGGGGAA AGCCACATGC CGCTGTGCCT CGGGCTTCAC GGGCGAGGAC
    TGCCAGCACT CGACTACTCA CCCCTGCTTT GTGTCTCACC CCTGCCTGAA TGGGGGCACC
    TGCCACGTGC TTAGCCGGGA CACCTACGAG TGCACCTGCC AAGTCGGTTT TACAGGTAAA
    CTGTGCCAGT GGACTGATGC CTGCCTGTCC CACCCCTGTG CAAACGGAAG TACCTGTACC
    ACTATGGCCA ACCAGTTCTC CTGCACATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG
    ACTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ACGGTGGCAC CTGCCTCAAC
    CTCCCGGGCT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCCAGCA CTGCGACAGT
    CCCTACGTGC CCTGCGCACC CTCCCCCTGT GTCAACGGAG GTACCTGCCG GCAAACCGGT
    GACTTTACCT TTGAGTGCAG TTGCCTTCCA GGTTTTGAAG GAGTCACCTG TGAGCGGAAT
    ATAGATGACT GTCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAT
    ACCTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGCCTGC TGCAACCCAA TGCCTGTCAG AACGGGGGCA CCTGTACCAA CCGCAACGGG
    GGCTATGGCT GTGTATGTGT GAATGGCTGG AGTGGGGACG ACTGCAGTGA GAATATCGAT
    GACTGTGCCT TCGCCTCCTG CACCCCGGGC TCTACTTGCA TCGATCGTGT GGCCTCCTTC
    TCTTGTATGT GCCCAGAAGG AAAGGCAGGT CTCTTATGTC ATCTGGATGA CGCGTGCATC
    AGCAACCCCT GCCACAAGGG GGCGCTGTGT GATACCAACC CTCTGAATGG ACAGTATATT
    TGCACCTGCC CACAAGGCTA CAAAGGGAGT GACTGTACAG AAGATGTGGA TGAGTGTGCC
    ATGGCCAACA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAGT GTCTGAAGGG CTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC
    CACTCAGACC CCTGCAAGAA TGATGCTACC TGTTTGGATA AGATTGGAGG CTTCACGTGT
    CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAGTTGG AGATAAATGA ATGTCAGAGC
    AACCCCTGTG TGAACAATGG ACAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTGTGT
    CCGCCTGGAT TCACGGGGCC AGTTTGCCAG ATCGATATTG ACGACTGTTC TAGTACTCCG
    TGTCTGAATG GGGCAAAGTG TATAGATCAC CCGAATGGCT ATGAGTGCCA GTGTGCCACA
    GGGTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC
    CACGGCCAGT GTCAGGACGG CATTGATTCC TACACCTGCA TCTGCAGTCC GGGCTACATG
    GGCGCCATCT GCAGTGACCA GATAGACGAA TGCTACAGCA GCCCTTGCCT GAATGAGGGT
    CGCTGCATCG ACCTGGTGAA TGGTTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGTGTT
    AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCGT GTGTCCATGG AGTCTGTATG
    GATGGCGTTA ATCGTTACAG CTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGCAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG CATCAATGAT
    GTGAACGGTT TCCGCTGTAT CTGCCCTGAG GGCCCCCACC ATCCCAGCTG CTACTCACAG
    GTGAACGAGT GCCTGAGCAA TCCCTGTGTC CACGGAAACT GTACTGGGGG CCTCACTGGA
    TACAAGTGCC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA
    TGTCTTTCTA ATCCATGCCA GAACGGGGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGCACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCT
    TCAAATCCAT GTCTGAACCA AGGAACCTGC CTTGATGACG TAAGCGGTTA TACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAACTGT CAGACCGTAT TGGCTCCCTG TTCCCCAAAC
    CCTTGTGAGA ATGCTGGTGT TTGCAAAGAG GCACCAAATT TTGAGAGTTA CTCCTGCCTG
    TGTGCTCCTG GCTGGAAAGG TCAGCGGTGT ACCATCGACA TTGATGAGTG TGTCTCCAAG
    CCCTGCATGA ACCACGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCT
    CCAGGCTTCA GTGGCATGGA CTGTGAGGAG GACGTTGATG ACTGCCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG CCTTCCGGGC
    TTCACTGGGG ATAAATGTCA GACAGACATG AATGAGTGTC TGAGTGAGCC CTGTAAGAAC
    GGAGGGACCT GCTCTGACTA TGTCAACAGT TACACCTGCA AGTGCCCGGC GGGATTTGAT
    GGAGTCCACT GTGAGAACAA CATTGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACGTGTATCG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGCTT CACCGGTTCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCATCCAT GCCTGAATGA AGGAGTATGT
    GTTGATGGCC TGGGTACCTA CCGCTGTATC TGCCCATTGG GCTACACTGG AAAAAACTGT
    CAGACCCTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC CTGCATTCAG
    GATAAAGCAG AGTCACGGTG CCTGTGTCCG TCTGGATGGG CTGGTGCTTA CTGCGATGTT
    CCCAGTGTCT CCTGTGAGGT GGCGGCCTCC CACAGAGGGG TGCCCGTTGA CCGCTTGTGC
    CAGCACTCAG GTGTCTGTAT CAGTGCGGGC AACTCGCATC ACTGCCAGTG CCCCTTGGGC
    TACACCGGGA GCTACTGTGA GGACCAGCTT GATGAGTGCT CGTCCAATCC CTGCCAGCAC
    GGCGCCACCT GCCGGGACTT CATTGGTGGA TACAGATGTG AGTGTGTCCC TGGATATCAG
    GGTGTCAACT GCGAGTATGA AGTGGATGAG TGCCAGAACC AGCCATGCCA GAATGGGGGC
    ACCTGTATCG ACCTTGTGAA CCACTTCAAG TGCTCCTGCC CACCAGGCAC TCGGGGCCTG
    CTTTGTGAAG AGAACGTTGA CGACTGTGCC AGGGGTCCCC ACTGCCTTAA TGGTGGCCAG
    TGTGTAGACA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CAGGCTTTGC TGGAGAACGC
    TGTGAGGGGG ACATCAACGA GTGCCTGTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGCATCCAGC TCACCAACGA CTACCTGTGC GTCTGCCGCA GCACCTTCAC TGGTCGTCAC
    TGTGAAACGT TCGTCGACGT GTGTCCCCAG ATGCCTTGCC TGAACGGAGG CACTTGTGCT
    GTGGCCAGCA ACATGCCCGA TGGTTTCATC TGTCGTTGTC CCCCGGGCTT CTCTGGGGCG
    CGGTGCCAGA GCAGCTGCGG ACAGGTGAAG TGCAGGAGAG GGGAGCAGTG TGTGCACACA
    GCCTCGGGCC CCCGCTGCTT CTGTCCCAAC CCCCAGGACT GCGACTCGGG CTGCGCCAGT
    AGCCCCTGCC AGCACGGGGG GAGCTGCCGC CCTCAGCGCC AGCCTCCTTA CTACTCGTGC
    CAGTGTGCCC CGCCGTTCCG GGGCAGCCAC TGTGAACTCT ACACCGCCCC CACCAGCACC
    CCTCCCGCCA CCTGCCTGAG CCAGTACTGT GCCGACAAAG CTCGGGACGG CATCTGTGAT
    GACGTGTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGGG ACTGTTCCCT CACCATGGAG
    GACCCCTGGG CCAATTGCTC CTCCCCGCTG CCCTGCTGGG AGTACATCAA CAACCAGTGC
    GACGAGCTGT GCAACACAGC CGAGTGCCTG TTTGACAACT TTGAATGCCA GAAGAGTAGC
    AAGACATGCA AATATGACAA ATACTGTGCA GACCACTACG AAGACAACCA TTGTGACCAG
    GGTTGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAA
    AACCTGGCAG AGGGCACCCT GGTCATCGTG GTGCTGATGC CACCTGAACA ACTGCTCCAG
    GATGCCCGCA GATTCTTGCG GGCACTGGGC ACCCTGCTCC ACACCAACCT CCGCATCAAA
    CGGGATGCCC AGGGGGAACT CATGGTCTAC CCCTATTATG GTGGGAAGTC AGCTGCCATG
    AAGAAGCAGA GATTGACACG CAGGTCCCTT CCTGGAGATG AAGAACAGGA AGTGGCTGGC
    TCTCAGGTAT TTCTGGAGAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCAGTGCTTT
    AAGAACACAG ATGCAGCCGC AGCTCTTCTG GCTTCTCACG CCATCCAGGG GACCCTGTCG
    TACCCACTTG TGTCTGTCGT CAGTGAGTCT CTGACCCACA AACGCACTCA GCTTCTCTAT
    CTGCTTGCTG TTGCCGTTGT CATTATCTTG TTTATTATCC TGCTGGGGGT TATCATGGCA
    AAACGAAAGC GCAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGTGAC
    TCCAGCAACC ACAAACGTCG TGAGCCAGTG GGCCAGGATG CTGTGGGACT CAAAAATCTC
    TCAGTGCAAG TCTCAGAGGC GAACCTAATT GGTTCTGGAA CAAGTGAACA CTGGGTCGAC
    GACGAAGGGC CGCAGCCTAA GAAAGCCAAG GCTGAAGATG AGGCTTTGCT CTCAGAAGAA
    GATGACCCCA TCGATCGGCG TCCGTGGACA CAGCAGCACC TGGAAGCTGC AGACATCCGT
    AGGACACCAT CGCTGGCTCT TACCCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT
    GTGAACGTCC GTGGTCCAGA TGGGTGCACC CCACTGATGC TGGCTTCTCT CCGAGGAGGC
    AGCTCAGACA TGAGTGATGA AGATGAAGAC ACAGAGGACT CTTCTGCCAA CATCATCACG
    GACTTAGTCT ATCAGGGTGC CAGCCTCCAA GCACAGACAG ACCGGACGGG TGAGATGGCC
    CTGCATCTCG CAGCCCGGTA CTCACGGGCA GATGCTGCCA AGCGTCTCCT GGATGCAGGT
    GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGCCCTC TCCATGCTGC AGTGGCCGCT
    GATGCCCAAG GTGTCTTCCA GATACTGATC CGCAACCGAG TAACTGATCT AGACGCAAGG
    ATGAATGATG GCACTACACC CCTGATCCTG GCTGCCCGCC TGGCAGTGGA GGGAATGGTG
    GCAGAACTGA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCC
    CTTCACTGGG CAGCTGCTGT GAATAATGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAGGA CAACAAGGAG GAGACACCCC TGTTTCTTGC TGCTCGGGAG
    GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGGGA CATCACAGAC
    CACATGGATC GCCTTCCCCG GGACGTAGCC CGGGACCGCA TGCACCATGA CATCGTGCGC
    CTGCTGGACG AGTACAACGT GACCCCCAGC CCTCCCGGCA CAGTGCTGAC GTCTGCGCTC
    TCACCCGTCA TCTGTGGGCC CAACAGACCT TTTCTCAGTT TGAAGCATAC CCCAATGGGC
    AAGAAGTCTA GACGGCCTAA CACCAAGAGT ACCGTGCCCA CTAGCCTCCC TAACCTTGCC
    AAGGAGGCCA AGGATGCCAA GGGGAACAGG AGGAAGAAGT CTCTGAGTGA CAAGGGCCAG
    CTGTCCGAGA GCTCAGTGAC TCTGTCCCCC GTCGATTCCC TAGAATCTCC GCACACATAT
    GTTTCTGACA CCACCTCCTC TCCAATGATC ACATCCCCCG GAATCTTACA GGCCTCCCCC
    AACCCCATGC TGGCCACTGC TGCCCCCACT GCCCCAGTCC ATGCACAGCA TGCACTGTCT
    TTCTCTAACC TTCATGAGAT GCAGCCCTTG GCTCATGGGG CCAACACTGT GCTTCCCTCA
    GTGAGCCAGC TGCTGTCTCA CCACCACATT GTGCCCCCAG GCAGCAGCAC CGCAGGGAGC
    TTGGGTAGGC TACATTCAGT CACTGTCCCG GCCGACTGGA TGAACCGCGT GGAGATGAAT
    GAGAGCCAGT ACAACGAGAT GTTCGGTATG GTCCTGGCTC CAGCTGAGGG CACCCACCCT
    GGCATAGCTC CCCAGAGCAG GCCGCCCGAA GGGAAGCATT TAACCACCCC CCGAGAGCCC
    TTGCCCCCCA TTGTGACTTT CCAGCTAATC CCCAAAGGCA GCATTGCCCA ACCAACTGGG
    GCTGCCCAGC CTCCGTCCAA CTGCCCTCCA GCCGTTGCAG GCACCCTGCC CACCATGTAT
    CAGATTCCAG AAATGGCTCG CTTGCCCAGT ATGGCTTTTC CCACTGCCAT GATGCCCCAG
    CAGGACGGGC AGGTTGCTCA GACCATTCTC CCAGCCTATC ATGCCTTCCC AGCCTCTGTG
    GGCAAGTATC CCACACCCCC ATCACAGCAT AGCTATGCAT CCTCCAATGC TGCTGAGAGA
    ACGCCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCACCCCT ACCTGACACC GTCCCCAGAG
    TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC
    ACCAGCCCTA CTCCTGGGGG CGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT
    GAGCCACCAC GCAGCAACAT GCAGGTCTAT GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006049408.1
    CDS247..7662
    Translation

    Target ORF information:

    RefSeq Version XM_006049346.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis notch 2 (NOTCH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006049346.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCCCGCCC TGCGCCCCGC TCCGCTGTGG GCGCTGCTGG CACTGTGGCT GTGCCGCGCG 
    GCCCCGGCGC GTGCATTGCA ATGTCGAGAT GGCTATGAGC CTTGTGTAAA TGAAGGAATA
    TGTGTTACCT ACCACAACGG TACAGGATAC TGCAAGTGTC CAGAGGGCTT CTTGGGAGAA
    TATTGTCAGC ATCGAGACCC CTGTGAGAAG AACCGTTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCTA TGTTGGGGAA AGCCACATGC CGCTGTGCCT CGGGCTTCAC GGGCGAGGAC
    TGCCAGCACT CGACTACTCA CCCCTGCTTT GTGTCTCACC CCTGCCTGAA TGGGGGCACC
    TGCCACGTGC TTAGCCGGGA CACCTACGAG TGCACCTGCC AAGTCGGTTT TACAGGTAAA
    CTGTGCCAGT GGACTGATGC CTGCCTGTCC CACCCCTGTG CAAACGGAAG TACCTGTACC
    ACTATGGCCA ACCAGTTCTC CTGCACATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG
    ACTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ACGGTGGCAC CTGCCTCAAC
    CTCCCGGGCT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCCAGCA CTGCGACAGT
    CCCTACGTGC CCTGCGCACC CTCCCCCTGT GTCAACGGAG GTACCTGCCG GCAAACCGGT
    GACTTTACCT TTGAGTGCAG TTGCCTTCCA GGTTTTGAAG GAGTCACCTG TGAGCGGAAT
    ATAGATGACT GTCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAT
    ACCTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGCCTGC TGCAACCCAA TGCCTGTCAG AACGGGGGCA CCTGTACCAA CCGCAACGGG
    GGCTATGGCT GTGTATGTGT GAATGGCTGG AGTGGGGACG ACTGCAGTGA GAATATCGAT
    GACTGTGCCT TCGCCTCCTG CACCCCGGGC TCTACTTGCA TCGATCGTGT GGCCTCCTTC
    TCTTGTATGT GCCCAGAAGG AAAGGCAGGT CTCTTATGTC ATCTGGATGA CGCGTGCATC
    AGCAACCCCT GCCACAAGGG GGCGCTGTGT GATACCAACC CTCTGAATGG ACAGTATATT
    TGCACCTGCC CACAAGGCTA CAAAGGGAGT GACTGTACAG AAGATGTGGA TGAGTGTGCC
    ATGGCCAACA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAGT GTCTGAAGGG CTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC
    CACTCAGACC CCTGCAAGAA TGATGCTACC TGTTTGGATA AGATTGGAGG CTTCACGTGT
    CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAGTTGG AGATAAATGA ATGTCAGAGC
    AACCCCTGTG TGAACAATGG ACAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTGTGT
    CCGCCTGGAT TCACGGGGCC AGTTTGCCAG ATCGATATTG ACGACTGTTC TAGTACTCCG
    TGTCTGAATG GGGCAAAGTG TATAGATCAC CCGAATGGCT ATGAGTGCCA GTGTGCCACA
    GGGTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC
    CACGGCCAGT GTCAGGACGG CATTGATTCC TACACCTGCA TCTGCAGTCC GGGCTACATG
    GGCGCCATCT GCAGTGACCA GATAGACGAA TGCTACAGCA GCCCTTGCCT GAATGAGGGT
    CGCTGCATCG ACCTGGTGAA TGGTTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGTGTT
    AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCGT GTGTCCATGG AGTCTGTATG
    GATGGCGTTA ATCGTTACAG CTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGCAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG CATCAATGAT
    GTGAACGGTT TCCGCTGTAT CTGCCCTGAG GGCCCCCACC ATCCCAGCTG CTACTCACAG
    GTGAACGAGT GCCTGAGCAA TCCCTGTGTC CACGGAAACT GTACTGGGGG CCTCACTGGA
    TACAAGTGCC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA
    TGTCTTTCTA ATCCATGCCA GAACGGGGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGCACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCT
    TCAAATCCAT GTCTGAACCA AGGAACCTGC CTTGATGACG TAAGCGGTTA TACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAACTGT CAGACCGTAT TGGCTCCCTG TTCCCCAAAC
    CCTTGTGAGA ATGCTGGTGT TTGCAAAGAG GCACCAAATT TTGAGAGTTA CTCCTGCCTG
    TGTGCTCCTG GCTGGAAAGG TCAGCGGTGT ACCATCGACA TTGATGAGTG TGTCTCCAAG
    CCCTGCATGA ACCACGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCT
    CCAGGCTTCA GTGGCATGGA CTGTGAGGAG GACGTTGATG ACTGCCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG CCTTCCGGGC
    TTCACTGGGG ATAAATGTCA GACAGACATG AATGAGTGTC TGAGTGAGCC CTGTAAGAAC
    GGAGGGACCT GCTCTGACTA TGTCAACAGT TACACCTGCA AGTGCCCGGC GGGATTTGAT
    GGAGTCCACT GTGAGAACAA CATTGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACGTGTATCG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGCTT CACCGGTTCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCATCCAT GCCTGAATGA AGGAGTATGT
    GTTGATGGCC TGGGTACCTA CCGCTGTATC TGCCCATTGG GCTACACTGG AAAAAACTGT
    CAGACCCTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC CTGCATTCAG
    GATAAAGCAG AGTCACGGTG CCTGTGTCCG TCTGGATGGG CTGGTGCTTA CTGCGATGTT
    CCCAGTGTCT CCTGTGAGGT GGCGGCCTCC CACAGAGGGG TGCCCGTTGA CCGCTTGTGC
    CAGCACTCAG GTGTCTGTAT CAGTGCGGGC AACTCGCATC ACTGCCAGTG CCCCTTGGGC
    TACACCGGGA GCTACTGTGA GGACCAGCTT GATGAGTGCT CGTCCAATCC CTGCCAGCAC
    GGCGCCACCT GCCGGGACTT CATTGGTGGA TACAGATGTG AGTGTGTCCC TGGATATCAG
    GGTGTCAACT GCGAGTATGA AGTGGATGAG TGCCAGAACC AGCCATGCCA GAATGGGGGC
    ACCTGTATCG ACCTTGTGAA CCACTTCAAG TGCTCCTGCC CACCAGGCAC TCGGGGCCTG
    CTTTGTGAAG AGAACGTTGA CGACTGTGCC AGGGGTCCCC ACTGCCTTAA TGGTGGCCAG
    TGTGTAGACA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CAGGCTTTGC TGGAGAACGC
    TGTGAGGGGG ACATCAACGA GTGCCTGTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGCATCCAGC TCACCAACGA CTACCTGTGC GTCTGCCGCA GCACCTTCAC TGGTCGTCAC
    TGTGAAACGT TCGTCGACGT GTGTCCCCAG ATGCCTTGCC TGAACGGAGG CACTTGTGCT
    GTGGCCAGCA ACATGCCCGA TGGTTTCATC TGTCGTTGTC CCCCGGGCTT CTCTGGGGCG
    CGGTGCCAGA GCAGCTGCGG ACAGGTGAAG TGCAGGAGAG GGGAGCAGTG TGTGCACACA
    GCCTCGGGCC CCCGCTGCTT CTGTCCCAAC CCCCAGGACT GCGACTCGGG CTGCGCCAGT
    AGCCCCTGCC AGCACGGGGG GAGCTGCCGC CCTCAGCGCC AGCCTCCTTA CTACTCGTGC
    CAGTGTGCCC CGCCGTTCCG GGGCAGCCAC TGTGAACTCT ACACCGCCCC CACCAGCACC
    CCTCCCGCCA CCTGCCTGAG CCAGTACTGT GCCGACAAAG CTCGGGACGG CATCTGTGAT
    GACGTGTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGGG ACTGTTCCCT CACCATGGAG
    GACCCCTGGG CCAATTGCTC CTCCCCGCTG CCCTGCTGGG AGTACATCAA CAACCAGTGC
    GACGAGCTGT GCAACACAGC CGAGTGCCTG TTTGACAACT TTGAATGCCA GAAGAGTAGC
    AAGACATGCA AATATGACAA ATACTGTGCA GACCACTACG AAGACAACCA TTGTGACCAG
    GGTTGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAA
    AACCTGGCAG AGGGCACCCT GGTCATCGTG GTGCTGATGC CACCTGAACA ACTGCTCCAG
    GATGCCCGCA GATTCTTGCG GGCACTGGGC ACCCTGCTCC ACACCAACCT CCGCATCAAA
    CGGGATGCCC AGGGGGAACT CATGGTCTAC CCCTATTATG GTGGGAAGTC AGCTGCCATG
    AAGAAGCAGA GATTGACACG CAGGTCCCTT CCTGGAGATG AAGAACAGGA AGTGGCTGGC
    TCTCAGGTAT TTCTGGAGAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCAGTGCTTT
    AAGAACACAG ATGCAGCCGC AGCTCTTCTG GCTTCTCACG CCATCCAGGG GACCCTGTCG
    TACCCACTTG TGTCTGTCGT CAGTGAGTCT CTGACCCACA AACGCACTCA GCTTCTCTAT
    CTGCTTGCTG TTGCCGTTGT CATTATCTTG TTTATTATCC TGCTGGGGGT TATCATGGCA
    AAACGAAAGC GCAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGTGAC
    TCCAGCAACC ACAAACGTCG TGAGCCAGTG GGCCAGGATG CTGTGGGACT CAAAAATCTC
    TCAGTGCAAG TCTCAGAGGC GAACCTAATT GGTTCTGGAA CAAGTGAACA CTGGGTCGAC
    GACGAAGGGC CGCAGCCTAA GAAAGCCAAG GCTGAAGATG AGGCTTTGCT CTCAGAAGAA
    GATGACCCCA TCGATCGGCG TCCGTGGACA CAGCAGCACC TGGAAGCTGC AGACATCCGT
    AGGACACCAT CGCTGGCTCT TACCCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT
    GTGAACGTCC GTGGTCCAGA TGGGTGCACC CCACTGATGC TGGCTTCTCT CCGAGGAGGC
    AGCTCAGACA TGAGTGATGA AGATGAAGAC ACAGAGGACT CTTCTGCCAA CATCATCACG
    GACTTAGTCT ATCAGGGTGC CAGCCTCCAA GCACAGACAG ACCGGACGGG TGAGATGGCC
    CTGCATCTCG CAGCCCGGTA CTCACGGGCA GATGCTGCCA AGCGTCTCCT GGATGCAGGT
    GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGCCCTC TCCATGCTGC AGTGGCCGCT
    GATGCCCAAG GTGTCTTCCA GATACTGATC CGCAACCGAG TAACTGATCT AGACGCAAGG
    ATGAATGATG GCACTACACC CCTGATCCTG GCTGCCCGCC TGGCAGTGGA GGGAATGGTG
    GCAGAACTGA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCC
    CTTCACTGGG CAGCTGCTGT GAATAATGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAGGA CAACAAGGAG GAGACACCCC TGTTTCTTGC TGCTCGGGAG
    GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGGGA CATCACAGAC
    CACATGGATC GCCTTCCCCG GGACGTAGCC CGGGACCGCA TGCACCATGA CATCGTGCGC
    CTGCTGGACG AGTACAACGT GACCCCCAGC CCTCCCGGCA CAGTGCTGAC GTCTGCGCTC
    TCACCCGTCA TCTGTGGGCC CAACAGACCT TTTCTCAGTT TGAAGCATAC CCCAATGGGC
    AAGAAGTCTA GACGGCCTAA CACCAAGAGT ACCGTGCCCA CTAGCCTCCC TAACCTTGCC
    AAGGAGGCCA AGGATGCCAA GGGGAACAGG AGGAAGAAGT CTCTGAGTGA CAAGGGCCAG
    CTGTCCGAGA GCTCAGTGAC TCTGTCCCCC GTCGATTCCC TAGAATCTCC GCACACATAT
    GTTTCTGACA CCACCTCCTC TCCAATGATC ACATCCCCCG GAATCTTACA GGCCTCCCCC
    AACCCCATGC TGGCCACTGC TGCCCCCACT GCCCCAGTCC ATGCACAGCA TGCACTGTCT
    TTCTCTAACC TTCATGAGAT GCAGCCCTTG GCTCATGGGG CCAACACTGT GCTTCCCTCA
    GTGAGCCAGC TGCTGTCTCA CCACCACATT GTGCCCCCAG GCAGCAGCAC CGCAGGGAGC
    TTGGGTAGGC TACATTCAGT CACTGTCCCG GCCGACTGGA TGAACCGCGT GGAGATGAAT
    GAGAGCCAGT ACAACGAGAT GTTCGGTATG GTCCTGGCTC CAGCTGAGGG CACCCACCCT
    GGCATAGCTC CCCAGAGCAG GCCGCCCGAA GGGAAGCATT TAACCACCCC CCGAGAGCCC
    TTGCCCCCCA TTGTGACTTT CCAGCTAATC CCCAAAGGCA GCATTGCCCA ACCAACTGGG
    GCTGCCCAGC CTCCGTCCAA CTGCCCTCCA GCCGTTGCAG GCACCCTGCC CACCATGTAT
    CAGATTCCAG AAATGGCTCG CTTGCCCAGT ATGGCTTTTC CCACTGCCAT GATGCCCCAG
    CAGGACGGGC AGGTTGCTCA GACCATTCTC CCAGCCTATC ATGCCTTCCC AGCCTCTGTG
    GGCAAGTATC CCACACCCCC ATCACAGCAT AGCTATGCAT CCTCCAATGC TGCTGAGAGA
    ACGCCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCACCCCT ACCTGACACC GTCCCCAGAG
    TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC
    ACCAGCCCTA CTCCTGGGGG CGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT
    GAGCCACCAC GCAGCAACAT GCAGGTCTAT GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu07608
    Clone ID Related Accession (Same CDS sequence) XM_006049347.1
    Accession Version XM_006049347.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7383bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product neurogenic locus notch homolog protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784196.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTGGATCT GCCCAGGCGG CGGCGGCGGA GGAGGAGGCG AACGAGAAGA TGCCCGCCCT 
    GCGCCCCGCT CCGCTGTGGG CGCTGCTGGC ACTGTGGCTG TGCCGCGCGG CCCCGGCGCG
    TGGTGTCCAG AGGGCTTCTT GGGAGAATAT TGTCAGCATC GAGACCCCTG TGAGAAGAAC
    CGTTGCCAGA ATGGTGGGAC GTGTGTGGCC CAGGCTATGT TGGGGAAAGC CACATGCCGC
    TGTGCCTCGG GCTTCACGGG CGAGGACTGC CAGCACTCGA CTACTCACCC CTGCTTTGTG
    TCTCACCCCT GCCTGAATGG GGGCACCTGC CACGTGCTTA GCCGGGACAC CTACGAGTGC
    ACCTGCCAAG TCGGTTTTAC AGGTAAACTG TGCCAGTGGA CTGATGCCTG CCTGTCCCAC
    CCCTGTGCAA ACGGAAGTAC CTGTACCACT ATGGCCAACC AGTTCTCCTG CACATGCCTC
    ACAGGCTTCA CAGGGCAGAA ATGTGAGACT GATGTCAATG AGTGTGACAT TCCAGGACAG
    TGCCAGCACG GTGGCACCTG CCTCAACCTC CCGGGCTCCT ATCAGTGCCA GTGCCCCCAG
    GGCTTCACAG GCCAGCACTG CGACAGTCCC TACGTGCCCT GCGCACCCTC CCCCTGTGTC
    AACGGAGGTA CCTGCCGGCA AACCGGTGAC TTTACCTTTG AGTGCAGTTG CCTTCCAGGT
    TTTGAAGGAG TCACCTGTGA GCGGAATATA GATGACTGTC CTAACCACAA GTGTCAGAAT
    GGAGGGGTTT GTGTGGATGG GGTCAATACC TACAACTGCC GCTGTCCCCC TCAGTGGACA
    GGACAGTTCT GCACAGAGGA TGTGGATGAG TGCCTGCTGC AACCCAATGC CTGTCAGAAC
    GGGGGCACCT GTACCAACCG CAACGGGGGC TATGGCTGTG TATGTGTGAA TGGCTGGAGT
    GGGGACGACT GCAGTGAGAA TATCGATGAC TGTGCCTTCG CCTCCTGCAC CCCGGGCTCT
    ACTTGCATCG ATCGTGTGGC CTCCTTCTCT TGTATGTGCC CAGAAGGAAA GGCAGGTCTC
    TTATGTCATC TGGATGACGC GTGCATCAGC AACCCCTGCC ACAAGGGGGC GCTGTGTGAT
    ACCAACCCTC TGAATGGACA GTATATTTGC ACCTGCCCAC AAGGCTACAA AGGGAGTGAC
    TGTACAGAAG ATGTGGATGA GTGTGCCATG GCCAACAGCA ATCCTTGTGA GCATGCAGGA
    AAATGTGTGA ACACAGATGG TGCCTTCCAC TGTGAGTGTC TGAAGGGCTA TGCAGGACCT
    CGTTGTGAGA TGGACATCAA TGAGTGCCAC TCAGACCCCT GCAAGAATGA TGCTACCTGT
    TTGGATAAGA TTGGAGGCTT CACGTGTCTG TGCATGCCAG GTTTCAAAGG TGTGCATTGT
    GAGTTGGAGA TAAATGAATG TCAGAGCAAC CCCTGTGTGA ACAATGGACA GTGTGTGGAT
    AAAGTCAATC GCTTCCAGTG TCTGTGTCCG CCTGGATTCA CGGGGCCAGT TTGCCAGATC
    GATATTGACG ACTGTTCTAG TACTCCGTGT CTGAATGGGG CAAAGTGTAT AGATCACCCG
    AATGGCTATG AGTGCCAGTG TGCCACAGGG TTCACTGGTG TGTTGTGTGA GGAGAACATT
    GACAACTGTG ACCCTGATCC TTGCCACCAC GGCCAGTGTC AGGACGGCAT TGATTCCTAC
    ACCTGCATCT GCAGTCCGGG CTACATGGGC GCCATCTGCA GTGACCAGAT AGACGAATGC
    TACAGCAGCC CTTGCCTGAA TGAGGGTCGC TGCATCGACC TGGTGAATGG TTACCAGTGC
    AACTGCCAGC CAGGCACGTC AGGTGTTAAT TGTGAAATCA ATTTTGATGA CTGTGCAAGT
    AACCCGTGTG TCCATGGAGT CTGTATGGAT GGCGTTAATC GTTACAGCTG TGTCTGCTCA
    CCAGGATTCA CAGGGCAGAG ATGCAACATT GACATTGATG AGTGTGCCTC CAATCCCTGT
    CGCAAGGGTG CAACATGCAT CAATGATGTG AACGGTTTCC GCTGTATCTG CCCTGAGGGC
    CCCCACCATC CCAGCTGCTA CTCACAGGTG AACGAGTGCC TGAGCAATCC CTGTGTCCAC
    GGAAACTGTA CTGGGGGCCT CACTGGATAC AAGTGCCTCT GTGATGCAGG CTGGGTTGGC
    ATCAACTGTG AAGTGGACAA AAATGAATGT CTTTCTAATC CATGCCAGAA CGGGGGAACT
    TGTGACAATC TGGTGAATGG ATACAGGTGC ACTTGCAAGA AGGGCTTTAA AGGCTATAAC
    TGCCAGGTGA ATATTGATGA ATGTGCTTCA AATCCATGTC TGAACCAAGG AACCTGCCTT
    GATGACGTAA GCGGTTATAC TTGCCACTGT GTGCTGCCAT ACACAGGCAA GAACTGTCAG
    ACCGTATTGG CTCCCTGTTC CCCAAACCCT TGTGAGAATG CTGGTGTTTG CAAAGAGGCA
    CCAAATTTTG AGAGTTACTC CTGCCTGTGT GCTCCTGGCT GGAAAGGTCA GCGGTGTACC
    ATCGACATTG ATGAGTGTGT CTCCAAGCCC TGCATGAACC ACGGTCTCTG CCATAACACC
    CAGGGCAGCT ACATGTGTGA ATGTCCTCCA GGCTTCAGTG GCATGGACTG TGAGGAGGAC
    GTTGATGACT GCCTTGCCAA TCCTTGCCAG AATGGAGGTT CCTGTGTAGA TGGAGTGAAT
    ACTTTCTCCT GCCTGTGCCT TCCGGGCTTC ACTGGGGATA AATGTCAGAC AGACATGAAT
    GAGTGTCTGA GTGAGCCCTG TAAGAACGGA GGGACCTGCT CTGACTATGT CAACAGTTAC
    ACCTGCAAGT GCCCGGCGGG ATTTGATGGA GTCCACTGTG AGAACAACAT TGACGAGTGC
    ACTGAGAGCT CCTGTTTCAA TGGTGGCACG TGTATCGATG GGATTAACTC CTTCTCTTGC
    TTGTGCCCTG TGGGCTTCAC CGGTTCCTTC TGCCTCCATG AGATCAATGA ATGCAACTCT
    CATCCATGCC TGAATGAAGG AGTATGTGTT GATGGCCTGG GTACCTACCG CTGTATCTGC
    CCATTGGGCT ACACTGGAAA AAACTGTCAG ACCCTGGTGA ACCTCTGTAG TCGGTCTCCA
    TGTAAAAACA AAGGTACCTG CATTCAGGAT AAAGCAGAGT CACGGTGCCT GTGTCCGTCT
    GGATGGGCTG GTGCTTACTG CGATGTTCCC AGTGTCTCCT GTGAGGTGGC GGCCTCCCAC
    AGAGGGGTGC CCGTTGACCG CTTGTGCCAG CACTCAGGTG TCTGTATCAG TGCGGGCAAC
    TCGCATCACT GCCAGTGCCC CTTGGGCTAC ACCGGGAGCT ACTGTGAGGA CCAGCTTGAT
    GAGTGCTCGT CCAATCCCTG CCAGCACGGC GCCACCTGCC GGGACTTCAT TGGTGGATAC
    AGATGTGAGT GTGTCCCTGG ATATCAGGGT GTCAACTGCG AGTATGAAGT GGATGAGTGC
    CAGAACCAGC CATGCCAGAA TGGGGGCACC TGTATCGACC TTGTGAACCA CTTCAAGTGC
    TCCTGCCCAC CAGGCACTCG GGGCCTGCTT TGTGAAGAGA ACGTTGACGA CTGTGCCAGG
    GGTCCCCACT GCCTTAATGG TGGCCAGTGT GTAGACAGGA TTGGGGGCTA CAGTTGTCGC
    TGCTTGCCAG GCTTTGCTGG AGAACGCTGT GAGGGGGACA TCAACGAGTG CCTGTCCAAC
    CCCTGCAGCT CTGAGGGCAG CCTGGACTGC ATCCAGCTCA CCAACGACTA CCTGTGCGTC
    TGCCGCAGCA CCTTCACTGG TCGTCACTGT GAAACGTTCG TCGACGTGTG TCCCCAGATG
    CCTTGCCTGA ACGGAGGCAC TTGTGCTGTG GCCAGCAACA TGCCCGATGG TTTCATCTGT
    CGTTGTCCCC CGGGCTTCTC TGGGGCGCGG TGCCAGAGCA GCTGCGGACA GGTGAAGTGC
    AGGAGAGGGG AGCAGTGTGT GCACACAGCC TCGGGCCCCC GCTGCTTCTG TCCCAACCCC
    CAGGACTGCG ACTCGGGCTG CGCCAGTAGC CCCTGCCAGC ACGGGGGGAG CTGCCGCCCT
    CAGCGCCAGC CTCCTTACTA CTCGTGCCAG TGTGCCCCGC CGTTCCGGGG CAGCCACTGT
    GAACTCTACA CCGCCCCCAC CAGCACCCCT CCCGCCACCT GCCTGAGCCA GTACTGTGCC
    GACAAAGCTC GGGACGGCAT CTGTGATGAC GTGTGCAACA GCCATGCCTG CCAGTGGGAT
    GGGGGGGACT GTTCCCTCAC CATGGAGGAC CCCTGGGCCA ATTGCTCCTC CCCGCTGCCC
    TGCTGGGAGT ACATCAACAA CCAGTGCGAC GAGCTGTGCA ACACAGCCGA GTGCCTGTTT
    GACAACTTTG AATGCCAGAA GAGTAGCAAG ACATGCAAAT ATGACAAATA CTGTGCAGAC
    CACTACGAAG ACAACCATTG TGACCAGGGT TGCAACAGTG AGGAGTGTGG CTGGGATGGG
    CTGGACTGTG CTGCTGACCG GCCAGAAAAC CTGGCAGAGG GCACCCTGGT CATCGTGGTG
    CTGATGCCAC CTGAACAACT GCTCCAGGAT GCCCGCAGAT TCTTGCGGGC ACTGGGCACC
    CTGCTCCACA CCAACCTCCG CATCAAACGG GATGCCCAGG GGGAACTCAT GGTCTACCCC
    TATTATGGTG GGAAGTCAGC TGCCATGAAG AAGCAGAGAT TGACACGCAG GTCCCTTCCT
    GGAGATGAAG AACAGGAAGT GGCTGGCTCT CAGGTATTTC TGGAGATTGA CAACCGCCAG
    TGTGTTCAAG ACTCAGACCA GTGCTTTAAG AACACAGATG CAGCCGCAGC TCTTCTGGCT
    TCTCACGCCA TCCAGGGGAC CCTGTCGTAC CCACTTGTGT CTGTCGTCAG TGAGTCTCTG
    ACCCACAAAC GCACTCAGCT TCTCTATCTG CTTGCTGTTG CCGTTGTCAT TATCTTGTTT
    ATTATCCTGC TGGGGGTTAT CATGGCAAAA CGAAAGCGCA AGCATGGCTC CCTCTGGCTG
    CCTGAAGGTT TCACCCTTCG CCGTGACTCC AGCAACCACA AACGTCGTGA GCCAGTGGGC
    CAGGATGCTG TGGGACTCAA AAATCTCTCA GTGCAAGTCT CAGAGGCGAA CCTAATTGGT
    TCTGGAACAA GTGAACACTG GGTCGACGAC GAAGGGCCGC AGCCTAAGAA AGCCAAGGCT
    GAAGATGAGG CTTTGCTCTC AGAAGAAGAT GACCCCATCG ATCGGCGTCC GTGGACACAG
    CAGCACCTGG AAGCTGCAGA CATCCGTAGG ACACCATCGC TGGCTCTTAC CCCTCCACAG
    GCAGAGCAGG AGGTGGACGT GCTGGATGTG AACGTCCGTG GTCCAGATGG GTGCACCCCA
    CTGATGCTGG CTTCTCTCCG AGGAGGCAGC TCAGACATGA GTGATGAAGA TGAAGACACA
    GAGGACTCTT CTGCCAACAT CATCACGGAC TTAGTCTATC AGGGTGCCAG CCTCCAAGCA
    CAGACAGACC GGACGGGTGA GATGGCCCTG CATCTCGCAG CCCGGTACTC ACGGGCAGAT
    GCTGCCAAGC GTCTCCTGGA TGCAGGTGCA GATGCCAATG CCCAGGACAA CATGGGCCGC
    TGCCCTCTCC ATGCTGCAGT GGCCGCTGAT GCCCAAGGTG TCTTCCAGAT ACTGATCCGC
    AACCGAGTAA CTGATCTAGA CGCAAGGATG AATGATGGCA CTACACCCCT GATCCTGGCT
    GCCCGCCTGG CAGTGGAGGG AATGGTGGCA GAACTGATCA ACTGCCAAGC AGATGTGAAT
    GCAGTGGATG ACCATGGAAA ATCTGCCCTT CACTGGGCAG CTGCTGTGAA TAATGTGGAG
    GCAACCCTTT TGCTGTTGAA AAATGGGGCC AACCGAGACA TGCAGGACAA CAAGGAGGAG
    ACACCCCTGT TTCTTGCTGC TCGGGAGGGG AGCTATGAAG CAGCCAAGAT CCTGTTAGAC
    CATTTTGCCA ATCGGGACAT CACAGACCAC ATGGATCGCC TTCCCCGGGA CGTAGCCCGG
    GACCGCATGC ACCATGACAT CGTGCGCCTG CTGGACGAGT ACAACGTGAC CCCCAGCCCT
    CCCGGCACAG TGCTGACGTC TGCGCTCTCA CCCGTCATCT GTGGGCCCAA CAGACCTTTT
    CTCAGTTTGA AGCATACCCC AATGGGCAAG AAGTCTAGAC GGCCTAACAC CAAGAGTACC
    GTGCCCACTA GCCTCCCTAA CCTTGCCAAG GAGGCCAAGG ATGCCAAGGG GAACAGGAGG
    AAGAAGTCTC TGAGTGACAA GGGCCAGCTG TCCGAGAGCT CAGTGACTCT GTCCCCCGTC
    GATTCCCTAG AATCTCCGCA CACATATGTT TCTGACACCA CCTCCTCTCC AATGATCACA
    TCCCCCGGAA TCTTACAGGC CTCCCCCAAC CCCATGCTGG CCACTGCTGC CCCCACTGCC
    CCAGTCCATG CACAGCATGC ACTGTCTTTC TCTAACCTTC ATGAGATGCA GCCCTTGGCT
    CATGGGGCCA ACACTGTGCT TCCCTCAGTG AGCCAGCTGC TGTCTCACCA CCACATTGTG
    CCCCCAGGCA GCAGCACCGC AGGGAGCTTG GGTAGGCTAC ATTCAGTCAC TGTCCCGGCC
    GACTGGATGA ACCGCGTGGA GATGAATGAG AGCCAGTACA ACGAGATGTT CGGTATGGTC
    CTGGCTCCAG CTGAGGGCAC CCACCCTGGC ATAGCTCCCC AGAGCAGGCC GCCCGAAGGG
    AAGCATTTAA CCACCCCCCG AGAGCCCTTG CCCCCCATTG TGACTTTCCA GCTAATCCCC
    AAAGGCAGCA TTGCCCAACC AACTGGGGCT GCCCAGCCTC CGTCCAACTG CCCTCCAGCC
    GTTGCAGGCA CCCTGCCCAC CATGTATCAG ATTCCAGAAA TGGCTCGCTT GCCCAGTATG
    GCTTTTCCCA CTGCCATGAT GCCCCAGCAG GACGGGCAGG TTGCTCAGAC CATTCTCCCA
    GCCTATCATG CCTTCCCAGC CTCTGTGGGC AAGTATCCCA CACCCCCATC ACAGCATAGC
    TATGCATCCT CCAATGCTGC TGAGAGAACG CCCAGTCACA GTGGTCACCT CCAGGGTGAG
    CACCCCTACC TGACACCGTC CCCAGAGTCT CCTGACCAGT GGTCAAGTTC ATCACCCCAC
    TCTGCTTCTG ACTGGTCAGA TGTGACCACC AGCCCTACTC CTGGGGGCGC TGGAGGAGGT
    CAGCGGGGAC CTGGGACACA CATGTCTGAG CCACCACGCA GCAACATGCA GGTCTATGCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006049409.1
    CDS198..7580
    Translation

    Target ORF information:

    RefSeq Version XM_006049347.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis notch 2 (NOTCH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006049347.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTGGATCT GCCCAGGCGG CGGCGGCGGA GGAGGAGGCG AACGAGAAGA TGCCCGCCCT 
    GCGCCCCGCT CCGCTGTGGG CGCTGCTGGC ACTGTGGCTG TGCCGCGCGG CCCCGGCGCG
    TGGTGTCCAG AGGGCTTCTT GGGAGAATAT TGTCAGCATC GAGACCCCTG TGAGAAGAAC
    CGTTGCCAGA ATGGTGGGAC GTGTGTGGCC CAGGCTATGT TGGGGAAAGC CACATGCCGC
    TGTGCCTCGG GCTTCACGGG CGAGGACTGC CAGCACTCGA CTACTCACCC CTGCTTTGTG
    TCTCACCCCT GCCTGAATGG GGGCACCTGC CACGTGCTTA GCCGGGACAC CTACGAGTGC
    ACCTGCCAAG TCGGTTTTAC AGGTAAACTG TGCCAGTGGA CTGATGCCTG CCTGTCCCAC
    CCCTGTGCAA ACGGAAGTAC CTGTACCACT ATGGCCAACC AGTTCTCCTG CACATGCCTC
    ACAGGCTTCA CAGGGCAGAA ATGTGAGACT GATGTCAATG AGTGTGACAT TCCAGGACAG
    TGCCAGCACG GTGGCACCTG CCTCAACCTC CCGGGCTCCT ATCAGTGCCA GTGCCCCCAG
    GGCTTCACAG GCCAGCACTG CGACAGTCCC TACGTGCCCT GCGCACCCTC CCCCTGTGTC
    AACGGAGGTA CCTGCCGGCA AACCGGTGAC TTTACCTTTG AGTGCAGTTG CCTTCCAGGT
    TTTGAAGGAG TCACCTGTGA GCGGAATATA GATGACTGTC CTAACCACAA GTGTCAGAAT
    GGAGGGGTTT GTGTGGATGG GGTCAATACC TACAACTGCC GCTGTCCCCC TCAGTGGACA
    GGACAGTTCT GCACAGAGGA TGTGGATGAG TGCCTGCTGC AACCCAATGC CTGTCAGAAC
    GGGGGCACCT GTACCAACCG CAACGGGGGC TATGGCTGTG TATGTGTGAA TGGCTGGAGT
    GGGGACGACT GCAGTGAGAA TATCGATGAC TGTGCCTTCG CCTCCTGCAC CCCGGGCTCT
    ACTTGCATCG ATCGTGTGGC CTCCTTCTCT TGTATGTGCC CAGAAGGAAA GGCAGGTCTC
    TTATGTCATC TGGATGACGC GTGCATCAGC AACCCCTGCC ACAAGGGGGC GCTGTGTGAT
    ACCAACCCTC TGAATGGACA GTATATTTGC ACCTGCCCAC AAGGCTACAA AGGGAGTGAC
    TGTACAGAAG ATGTGGATGA GTGTGCCATG GCCAACAGCA ATCCTTGTGA GCATGCAGGA
    AAATGTGTGA ACACAGATGG TGCCTTCCAC TGTGAGTGTC TGAAGGGCTA TGCAGGACCT
    CGTTGTGAGA TGGACATCAA TGAGTGCCAC TCAGACCCCT GCAAGAATGA TGCTACCTGT
    TTGGATAAGA TTGGAGGCTT CACGTGTCTG TGCATGCCAG GTTTCAAAGG TGTGCATTGT
    GAGTTGGAGA TAAATGAATG TCAGAGCAAC CCCTGTGTGA ACAATGGACA GTGTGTGGAT
    AAAGTCAATC GCTTCCAGTG TCTGTGTCCG CCTGGATTCA CGGGGCCAGT TTGCCAGATC
    GATATTGACG ACTGTTCTAG TACTCCGTGT CTGAATGGGG CAAAGTGTAT AGATCACCCG
    AATGGCTATG AGTGCCAGTG TGCCACAGGG TTCACTGGTG TGTTGTGTGA GGAGAACATT
    GACAACTGTG ACCCTGATCC TTGCCACCAC GGCCAGTGTC AGGACGGCAT TGATTCCTAC
    ACCTGCATCT GCAGTCCGGG CTACATGGGC GCCATCTGCA GTGACCAGAT AGACGAATGC
    TACAGCAGCC CTTGCCTGAA TGAGGGTCGC TGCATCGACC TGGTGAATGG TTACCAGTGC
    AACTGCCAGC CAGGCACGTC AGGTGTTAAT TGTGAAATCA ATTTTGATGA CTGTGCAAGT
    AACCCGTGTG TCCATGGAGT CTGTATGGAT GGCGTTAATC GTTACAGCTG TGTCTGCTCA
    CCAGGATTCA CAGGGCAGAG ATGCAACATT GACATTGATG AGTGTGCCTC CAATCCCTGT
    CGCAAGGGTG CAACATGCAT CAATGATGTG AACGGTTTCC GCTGTATCTG CCCTGAGGGC
    CCCCACCATC CCAGCTGCTA CTCACAGGTG AACGAGTGCC TGAGCAATCC CTGTGTCCAC
    GGAAACTGTA CTGGGGGCCT CACTGGATAC AAGTGCCTCT GTGATGCAGG CTGGGTTGGC
    ATCAACTGTG AAGTGGACAA AAATGAATGT CTTTCTAATC CATGCCAGAA CGGGGGAACT
    TGTGACAATC TGGTGAATGG ATACAGGTGC ACTTGCAAGA AGGGCTTTAA AGGCTATAAC
    TGCCAGGTGA ATATTGATGA ATGTGCTTCA AATCCATGTC TGAACCAAGG AACCTGCCTT
    GATGACGTAA GCGGTTATAC TTGCCACTGT GTGCTGCCAT ACACAGGCAA GAACTGTCAG
    ACCGTATTGG CTCCCTGTTC CCCAAACCCT TGTGAGAATG CTGGTGTTTG CAAAGAGGCA
    CCAAATTTTG AGAGTTACTC CTGCCTGTGT GCTCCTGGCT GGAAAGGTCA GCGGTGTACC
    ATCGACATTG ATGAGTGTGT CTCCAAGCCC TGCATGAACC ACGGTCTCTG CCATAACACC
    CAGGGCAGCT ACATGTGTGA ATGTCCTCCA GGCTTCAGTG GCATGGACTG TGAGGAGGAC
    GTTGATGACT GCCTTGCCAA TCCTTGCCAG AATGGAGGTT CCTGTGTAGA TGGAGTGAAT
    ACTTTCTCCT GCCTGTGCCT TCCGGGCTTC ACTGGGGATA AATGTCAGAC AGACATGAAT
    GAGTGTCTGA GTGAGCCCTG TAAGAACGGA GGGACCTGCT CTGACTATGT CAACAGTTAC
    ACCTGCAAGT GCCCGGCGGG ATTTGATGGA GTCCACTGTG AGAACAACAT TGACGAGTGC
    ACTGAGAGCT CCTGTTTCAA TGGTGGCACG TGTATCGATG GGATTAACTC CTTCTCTTGC
    TTGTGCCCTG TGGGCTTCAC CGGTTCCTTC TGCCTCCATG AGATCAATGA ATGCAACTCT
    CATCCATGCC TGAATGAAGG AGTATGTGTT GATGGCCTGG GTACCTACCG CTGTATCTGC
    CCATTGGGCT ACACTGGAAA AAACTGTCAG ACCCTGGTGA ACCTCTGTAG TCGGTCTCCA
    TGTAAAAACA AAGGTACCTG CATTCAGGAT AAAGCAGAGT CACGGTGCCT GTGTCCGTCT
    GGATGGGCTG GTGCTTACTG CGATGTTCCC AGTGTCTCCT GTGAGGTGGC GGCCTCCCAC
    AGAGGGGTGC CCGTTGACCG CTTGTGCCAG CACTCAGGTG TCTGTATCAG TGCGGGCAAC
    TCGCATCACT GCCAGTGCCC CTTGGGCTAC ACCGGGAGCT ACTGTGAGGA CCAGCTTGAT
    GAGTGCTCGT CCAATCCCTG CCAGCACGGC GCCACCTGCC GGGACTTCAT TGGTGGATAC
    AGATGTGAGT GTGTCCCTGG ATATCAGGGT GTCAACTGCG AGTATGAAGT GGATGAGTGC
    CAGAACCAGC CATGCCAGAA TGGGGGCACC TGTATCGACC TTGTGAACCA CTTCAAGTGC
    TCCTGCCCAC CAGGCACTCG GGGCCTGCTT TGTGAAGAGA ACGTTGACGA CTGTGCCAGG
    GGTCCCCACT GCCTTAATGG TGGCCAGTGT GTAGACAGGA TTGGGGGCTA CAGTTGTCGC
    TGCTTGCCAG GCTTTGCTGG AGAACGCTGT GAGGGGGACA TCAACGAGTG CCTGTCCAAC
    CCCTGCAGCT CTGAGGGCAG CCTGGACTGC ATCCAGCTCA CCAACGACTA CCTGTGCGTC
    TGCCGCAGCA CCTTCACTGG TCGTCACTGT GAAACGTTCG TCGACGTGTG TCCCCAGATG
    CCTTGCCTGA ACGGAGGCAC TTGTGCTGTG GCCAGCAACA TGCCCGATGG TTTCATCTGT
    CGTTGTCCCC CGGGCTTCTC TGGGGCGCGG TGCCAGAGCA GCTGCGGACA GGTGAAGTGC
    AGGAGAGGGG AGCAGTGTGT GCACACAGCC TCGGGCCCCC GCTGCTTCTG TCCCAACCCC
    CAGGACTGCG ACTCGGGCTG CGCCAGTAGC CCCTGCCAGC ACGGGGGGAG CTGCCGCCCT
    CAGCGCCAGC CTCCTTACTA CTCGTGCCAG TGTGCCCCGC CGTTCCGGGG CAGCCACTGT
    GAACTCTACA CCGCCCCCAC CAGCACCCCT CCCGCCACCT GCCTGAGCCA GTACTGTGCC
    GACAAAGCTC GGGACGGCAT CTGTGATGAC GTGTGCAACA GCCATGCCTG CCAGTGGGAT
    GGGGGGGACT GTTCCCTCAC CATGGAGGAC CCCTGGGCCA ATTGCTCCTC CCCGCTGCCC
    TGCTGGGAGT ACATCAACAA CCAGTGCGAC GAGCTGTGCA ACACAGCCGA GTGCCTGTTT
    GACAACTTTG AATGCCAGAA GAGTAGCAAG ACATGCAAAT ATGACAAATA CTGTGCAGAC
    CACTACGAAG ACAACCATTG TGACCAGGGT TGCAACAGTG AGGAGTGTGG CTGGGATGGG
    CTGGACTGTG CTGCTGACCG GCCAGAAAAC CTGGCAGAGG GCACCCTGGT CATCGTGGTG
    CTGATGCCAC CTGAACAACT GCTCCAGGAT GCCCGCAGAT TCTTGCGGGC ACTGGGCACC
    CTGCTCCACA CCAACCTCCG CATCAAACGG GATGCCCAGG GGGAACTCAT GGTCTACCCC
    TATTATGGTG GGAAGTCAGC TGCCATGAAG AAGCAGAGAT TGACACGCAG GTCCCTTCCT
    GGAGATGAAG AACAGGAAGT GGCTGGCTCT CAGGTATTTC TGGAGATTGA CAACCGCCAG
    TGTGTTCAAG ACTCAGACCA GTGCTTTAAG AACACAGATG CAGCCGCAGC TCTTCTGGCT
    TCTCACGCCA TCCAGGGGAC CCTGTCGTAC CCACTTGTGT CTGTCGTCAG TGAGTCTCTG
    ACCCACAAAC GCACTCAGCT TCTCTATCTG CTTGCTGTTG CCGTTGTCAT TATCTTGTTT
    ATTATCCTGC TGGGGGTTAT CATGGCAAAA CGAAAGCGCA AGCATGGCTC CCTCTGGCTG
    CCTGAAGGTT TCACCCTTCG CCGTGACTCC AGCAACCACA AACGTCGTGA GCCAGTGGGC
    CAGGATGCTG TGGGACTCAA AAATCTCTCA GTGCAAGTCT CAGAGGCGAA CCTAATTGGT
    TCTGGAACAA GTGAACACTG GGTCGACGAC GAAGGGCCGC AGCCTAAGAA AGCCAAGGCT
    GAAGATGAGG CTTTGCTCTC AGAAGAAGAT GACCCCATCG ATCGGCGTCC GTGGACACAG
    CAGCACCTGG AAGCTGCAGA CATCCGTAGG ACACCATCGC TGGCTCTTAC CCCTCCACAG
    GCAGAGCAGG AGGTGGACGT GCTGGATGTG AACGTCCGTG GTCCAGATGG GTGCACCCCA
    CTGATGCTGG CTTCTCTCCG AGGAGGCAGC TCAGACATGA GTGATGAAGA TGAAGACACA
    GAGGACTCTT CTGCCAACAT CATCACGGAC TTAGTCTATC AGGGTGCCAG CCTCCAAGCA
    CAGACAGACC GGACGGGTGA GATGGCCCTG CATCTCGCAG CCCGGTACTC ACGGGCAGAT
    GCTGCCAAGC GTCTCCTGGA TGCAGGTGCA GATGCCAATG CCCAGGACAA CATGGGCCGC
    TGCCCTCTCC ATGCTGCAGT GGCCGCTGAT GCCCAAGGTG TCTTCCAGAT ACTGATCCGC
    AACCGAGTAA CTGATCTAGA CGCAAGGATG AATGATGGCA CTACACCCCT GATCCTGGCT
    GCCCGCCTGG CAGTGGAGGG AATGGTGGCA GAACTGATCA ACTGCCAAGC AGATGTGAAT
    GCAGTGGATG ACCATGGAAA ATCTGCCCTT CACTGGGCAG CTGCTGTGAA TAATGTGGAG
    GCAACCCTTT TGCTGTTGAA AAATGGGGCC AACCGAGACA TGCAGGACAA CAAGGAGGAG
    ACACCCCTGT TTCTTGCTGC TCGGGAGGGG AGCTATGAAG CAGCCAAGAT CCTGTTAGAC
    CATTTTGCCA ATCGGGACAT CACAGACCAC ATGGATCGCC TTCCCCGGGA CGTAGCCCGG
    GACCGCATGC ACCATGACAT CGTGCGCCTG CTGGACGAGT ACAACGTGAC CCCCAGCCCT
    CCCGGCACAG TGCTGACGTC TGCGCTCTCA CCCGTCATCT GTGGGCCCAA CAGACCTTTT
    CTCAGTTTGA AGCATACCCC AATGGGCAAG AAGTCTAGAC GGCCTAACAC CAAGAGTACC
    GTGCCCACTA GCCTCCCTAA CCTTGCCAAG GAGGCCAAGG ATGCCAAGGG GAACAGGAGG
    AAGAAGTCTC TGAGTGACAA GGGCCAGCTG TCCGAGAGCT CAGTGACTCT GTCCCCCGTC
    GATTCCCTAG AATCTCCGCA CACATATGTT TCTGACACCA CCTCCTCTCC AATGATCACA
    TCCCCCGGAA TCTTACAGGC CTCCCCCAAC CCCATGCTGG CCACTGCTGC CCCCACTGCC
    CCAGTCCATG CACAGCATGC ACTGTCTTTC TCTAACCTTC ATGAGATGCA GCCCTTGGCT
    CATGGGGCCA ACACTGTGCT TCCCTCAGTG AGCCAGCTGC TGTCTCACCA CCACATTGTG
    CCCCCAGGCA GCAGCACCGC AGGGAGCTTG GGTAGGCTAC ATTCAGTCAC TGTCCCGGCC
    GACTGGATGA ACCGCGTGGA GATGAATGAG AGCCAGTACA ACGAGATGTT CGGTATGGTC
    CTGGCTCCAG CTGAGGGCAC CCACCCTGGC ATAGCTCCCC AGAGCAGGCC GCCCGAAGGG
    AAGCATTTAA CCACCCCCCG AGAGCCCTTG CCCCCCATTG TGACTTTCCA GCTAATCCCC
    AAAGGCAGCA TTGCCCAACC AACTGGGGCT GCCCAGCCTC CGTCCAACTG CCCTCCAGCC
    GTTGCAGGCA CCCTGCCCAC CATGTATCAG ATTCCAGAAA TGGCTCGCTT GCCCAGTATG
    GCTTTTCCCA CTGCCATGAT GCCCCAGCAG GACGGGCAGG TTGCTCAGAC CATTCTCCCA
    GCCTATCATG CCTTCCCAGC CTCTGTGGGC AAGTATCCCA CACCCCCATC ACAGCATAGC
    TATGCATCCT CCAATGCTGC TGAGAGAACG CCCAGTCACA GTGGTCACCT CCAGGGTGAG
    CACCCCTACC TGACACCGTC CCCAGAGTCT CCTGACCAGT GGTCAAGTTC ATCACCCCAC
    TCTGCTTCTG ACTGGTCAGA TGTGACCACC AGCCCTACTC CTGGGGGCGC TGGAGGAGGT
    CAGCGGGGAC CTGGGACACA CATGTCTGAG CCACCACGCA GCAACATGCA GGTCTATGCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu07609
    Clone ID Related Accession (Same CDS sequence) XM_006049348.1
    Accession Version XM_006049348.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7302bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product neurogenic locus notch homolog protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784196.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCCCGCCC TGCGCCCCGC TCCGCTGTGG GCGCTGCTGG CACTGTGGCT GTGCCGCGCG 
    GCCCCGGCGC GTGCATTGCA ATGTCGAGAT GGCTATGAGC CTTGTGTAAA TGAAGGAATA
    TGTGTTACCT ACCACAACGG TACAGGATAC TGCAAGTGTC CAGAGGGCTT CTTGGGAGAA
    TATTGTCAGC ATCGAGACCC CTGTGAGAAG AACCGTTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCTA TGTTGGGGAA AGCCACATGC CGCTGTGCCT CGGGCTTCAC GGGCGAGGAC
    TGCCAGCACT CGACTACTCA CCCCTGCTTT GTGTCTCACC CCTGCCTGAA TGGGGGCACC
    TGCCACGTGC TTAGCCGGGA CACCTACGAG TGCACCTGCC AAGTCGGTTT TACAGGTAAA
    CTGTGCCAGT GGACTGATGC CTGCCTGTCC CACCCCTGTG CAAACGGAAG TACCTGTACC
    ACTATGGCCA ACCAGTTCTC CTGCACATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG
    ACTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ACGGTGGCAC CTGCCTCAAC
    CTCCCGGGCT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCCAGCA CTGCGACAGT
    CCCTACGTGC CCTGCGCACC CTCCCCCTGT GTCAACGGAG GTACCTGCCG GCAAACCGGT
    GACTTTACCT TTGAGTGCAG TTGCCTTCCA GGTTTTGAAG GAGTCACCTG TGAGCGGAAT
    ATAGATGACT GTCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAT
    ACCTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGCCTGC TGCAACCCAA TGCCTGTCAG AACGGGGGCA CCTGTACCAA CCGCAACGGG
    GGCTATGGCT GTGTATGTGT GAATGGCTGG AGTGGGGACG ACTGCAGTGA GAATATCGAT
    GACTGTGCCT TCGCCTCCTG CACCCCGGGC TCTACTTGCA TCGATCGTGT GGCCTCCTTC
    TCTTGTATGT GCCCAGAAGG AAAGGCAGGT CTCTTATGTC ATCTGGATGA CGCGTGCATC
    AGCAACCCCT GCCACAAGGG GGCGCTGTGT GATACCAACC CTCTGAATGG ACAGTATATT
    TGCACCTGCC CACAAGGCTA CAAAGGGAGT GACTGTACAG AAGATGTGGA TGAGTGTGCC
    ATGGCCAACA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAGT GTCTGAAGGG CTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC
    CACTCAGACC CCTGCAAGAA TGATGCTACC TGTTTGGATA AGATTGGAGG CTTCACGTGT
    CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAGTTGG AGATAAATGA ATGTCAGAGC
    AACCCCTGTG TGAACAATGG ACAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTGTGT
    CCGCCTGGGT TCACTGGTGT GTTGTGTGAG GAGAACATTG ACAACTGTGA CCCTGATCCT
    TGCCACCACG GCCAGTGTCA GGACGGCATT GATTCCTACA CCTGCATCTG CAGTCCGGGC
    TACATGGGCG CCATCTGCAG TGACCAGATA GACGAATGCT ACAGCAGCCC TTGCCTGAAT
    GAGGGTCGCT GCATCGACCT GGTGAATGGT TACCAGTGCA ACTGCCAGCC AGGCACGTCA
    GGTGTTAATT GTGAAATCAA TTTTGATGAC TGTGCAAGTA ACCCGTGTGT CCATGGAGTC
    TGTATGGATG GCGTTAATCG TTACAGCTGT GTCTGCTCAC CAGGATTCAC AGGGCAGAGA
    TGCAACATTG ACATTGATGA GTGTGCCTCC AATCCCTGTC GCAAGGGTGC AACATGCATC
    AATGATGTGA ACGGTTTCCG CTGTATCTGC CCTGAGGGCC CCCACCATCC CAGCTGCTAC
    TCACAGGTGA ACGAGTGCCT GAGCAATCCC TGTGTCCACG GAAACTGTAC TGGGGGCCTC
    ACTGGATACA AGTGCCTCTG TGATGCAGGC TGGGTTGGCA TCAACTGTGA AGTGGACAAA
    AATGAATGTC TTTCTAATCC ATGCCAGAAC GGGGGAACTT GTGACAATCT GGTGAATGGA
    TACAGGTGCA CTTGCAAGAA GGGCTTTAAA GGCTATAACT GCCAGGTGAA TATTGATGAA
    TGTGCTTCAA ATCCATGTCT GAACCAAGGA ACCTGCCTTG ATGACGTAAG CGGTTATACT
    TGCCACTGTG TGCTGCCATA CACAGGCAAG AACTGTCAGA CCGTATTGGC TCCCTGTTCC
    CCAAACCCTT GTGAGAATGC TGGTGTTTGC AAAGAGGCAC CAAATTTTGA GAGTTACTCC
    TGCCTGTGTG CTCCTGGCTG GAAAGGTCAG CGGTGTACCA TCGACATTGA TGAGTGTGTC
    TCCAAGCCCT GCATGAACCA CGGTCTCTGC CATAACACCC AGGGCAGCTA CATGTGTGAA
    TGTCCTCCAG GCTTCAGTGG CATGGACTGT GAGGAGGACG TTGATGACTG CCTTGCCAAT
    CCTTGCCAGA ATGGAGGTTC CTGTGTAGAT GGAGTGAATA CTTTCTCCTG CCTGTGCCTT
    CCGGGCTTCA CTGGGGATAA ATGTCAGACA GACATGAATG AGTGTCTGAG TGAGCCCTGT
    AAGAACGGAG GGACCTGCTC TGACTATGTC AACAGTTACA CCTGCAAGTG CCCGGCGGGA
    TTTGATGGAG TCCACTGTGA GAACAACATT GACGAGTGCA CTGAGAGCTC CTGTTTCAAT
    GGTGGCACGT GTATCGATGG GATTAACTCC TTCTCTTGCT TGTGCCCTGT GGGCTTCACC
    GGTTCCTTCT GCCTCCATGA GATCAATGAA TGCAACTCTC ATCCATGCCT GAATGAAGGA
    GTATGTGTTG ATGGCCTGGG TACCTACCGC TGTATCTGCC CATTGGGCTA CACTGGAAAA
    AACTGTCAGA CCCTGGTGAA CCTCTGTAGT CGGTCTCCAT GTAAAAACAA AGGTACCTGC
    ATTCAGGATA AAGCAGAGTC ACGGTGCCTG TGTCCGTCTG GATGGGCTGG TGCTTACTGC
    GATGTTCCCA GTGTCTCCTG TGAGGTGGCG GCCTCCCACA GAGGGGTGCC CGTTGACCGC
    TTGTGCCAGC ACTCAGGTGT CTGTATCAGT GCGGGCAACT CGCATCACTG CCAGTGCCCC
    TTGGGCTACA CCGGGAGCTA CTGTGAGGAC CAGCTTGATG AGTGCTCGTC CAATCCCTGC
    CAGCACGGCG CCACCTGCCG GGACTTCATT GGTGGATACA GATGTGAGTG TGTCCCTGGA
    TATCAGGGTG TCAACTGCGA GTATGAAGTG GATGAGTGCC AGAACCAGCC ATGCCAGAAT
    GGGGGCACCT GTATCGACCT TGTGAACCAC TTCAAGTGCT CCTGCCCACC AGGCACTCGG
    GGCCTGCTTT GTGAAGAGAA CGTTGACGAC TGTGCCAGGG GTCCCCACTG CCTTAATGGT
    GGCCAGTGTG TAGACAGGAT TGGGGGCTAC AGTTGTCGCT GCTTGCCAGG CTTTGCTGGA
    GAACGCTGTG AGGGGGACAT CAACGAGTGC CTGTCCAACC CCTGCAGCTC TGAGGGCAGC
    CTGGACTGCA TCCAGCTCAC CAACGACTAC CTGTGCGTCT GCCGCAGCAC CTTCACTGGT
    CGTCACTGTG AAACGTTCGT CGACGTGTGT CCCCAGATGC CTTGCCTGAA CGGAGGCACT
    TGTGCTGTGG CCAGCAACAT GCCCGATGGT TTCATCTGTC GTTGTCCCCC GGGCTTCTCT
    GGGGCGCGGT GCCAGAGCAG CTGCGGACAG GTGAAGTGCA GGAGAGGGGA GCAGTGTGTG
    CACACAGCCT CGGGCCCCCG CTGCTTCTGT CCCAACCCCC AGGACTGCGA CTCGGGCTGC
    GCCAGTAGCC CCTGCCAGCA CGGGGGGAGC TGCCGCCCTC AGCGCCAGCC TCCTTACTAC
    TCGTGCCAGT GTGCCCCGCC GTTCCGGGGC AGCCACTGTG AACTCTACAC CGCCCCCACC
    AGCACCCCTC CCGCCACCTG CCTGAGCCAG TACTGTGCCG ACAAAGCTCG GGACGGCATC
    TGTGATGACG TGTGCAACAG CCATGCCTGC CAGTGGGATG GGGGGGACTG TTCCCTCACC
    ATGGAGGACC CCTGGGCCAA TTGCTCCTCC CCGCTGCCCT GCTGGGAGTA CATCAACAAC
    CAGTGCGACG AGCTGTGCAA CACAGCCGAG TGCCTGTTTG ACAACTTTGA ATGCCAGAAG
    AGTAGCAAGA CATGCAAATA TGACAAATAC TGTGCAGACC ACTACGAAGA CAACCATTGT
    GACCAGGGTT GCAACAGTGA GGAGTGTGGC TGGGATGGGC TGGACTGTGC TGCTGACCGG
    CCAGAAAACC TGGCAGAGGG CACCCTGGTC ATCGTGGTGC TGATGCCACC TGAACAACTG
    CTCCAGGATG CCCGCAGATT CTTGCGGGCA CTGGGCACCC TGCTCCACAC CAACCTCCGC
    ATCAAACGGG ATGCCCAGGG GGAACTCATG GTCTACCCCT ATTATGGTGG GAAGTCAGCT
    GCCATGAAGA AGCAGAGATT GACACGCAGG TCCCTTCCTG GAGATGAAGA ACAGGAAGTG
    GCTGGCTCTC AGGTATTTCT GGAGATTGAC AACCGCCAGT GTGTTCAAGA CTCAGACCAG
    TGCTTTAAGA ACACAGATGC AGCCGCAGCT CTTCTGGCTT CTCACGCCAT CCAGGGGACC
    CTGTCGTACC CACTTGTGTC TGTCGTCAGT GAGTCTCTGA CCCACAAACG CACTCAGCTT
    CTCTATCTGC TTGCTGTTGC CGTTGTCATT ATCTTGTTTA TTATCCTGCT GGGGGTTATC
    ATGGCAAAAC GAAAGCGCAA GCATGGCTCC CTCTGGCTGC CTGAAGGTTT CACCCTTCGC
    CGTGACTCCA GCAACCACAA ACGTCGTGAG CCAGTGGGCC AGGATGCTGT GGGACTCAAA
    AATCTCTCAG TGCAAGTCTC AGAGGCGAAC CTAATTGGTT CTGGAACAAG TGAACACTGG
    GTCGACGACG AAGGGCCGCA GCCTAAGAAA GCCAAGGCTG AAGATGAGGC TTTGCTCTCA
    GAAGAAGATG ACCCCATCGA TCGGCGTCCG TGGACACAGC AGCACCTGGA AGCTGCAGAC
    ATCCGTAGGA CACCATCGCT GGCTCTTACC CCTCCACAGG CAGAGCAGGA GGTGGACGTG
    CTGGATGTGA ACGTCCGTGG TCCAGATGGG TGCACCCCAC TGATGCTGGC TTCTCTCCGA
    GGAGGCAGCT CAGACATGAG TGATGAAGAT GAAGACACAG AGGACTCTTC TGCCAACATC
    ATCACGGACT TAGTCTATCA GGGTGCCAGC CTCCAAGCAC AGACAGACCG GACGGGTGAG
    ATGGCCCTGC ATCTCGCAGC CCGGTACTCA CGGGCAGATG CTGCCAAGCG TCTCCTGGAT
    GCAGGTGCAG ATGCCAATGC CCAGGACAAC ATGGGCCGCT GCCCTCTCCA TGCTGCAGTG
    GCCGCTGATG CCCAAGGTGT CTTCCAGATA CTGATCCGCA ACCGAGTAAC TGATCTAGAC
    GCAAGGATGA ATGATGGCAC TACACCCCTG ATCCTGGCTG CCCGCCTGGC AGTGGAGGGA
    ATGGTGGCAG AACTGATCAA CTGCCAAGCA GATGTGAATG CAGTGGATGA CCATGGAAAA
    TCTGCCCTTC ACTGGGCAGC TGCTGTGAAT AATGTGGAGG CAACCCTTTT GCTGTTGAAA
    AATGGGGCCA ACCGAGACAT GCAGGACAAC AAGGAGGAGA CACCCCTGTT TCTTGCTGCT
    CGGGAGGGGA GCTATGAAGC AGCCAAGATC CTGTTAGACC ATTTTGCCAA TCGGGACATC
    ACAGACCACA TGGATCGCCT TCCCCGGGAC GTAGCCCGGG ACCGCATGCA CCATGACATC
    GTGCGCCTGC TGGACGAGTA CAACGTGACC CCCAGCCCTC CCGGCACAGT GCTGACGTCT
    GCGCTCTCAC CCGTCATCTG TGGGCCCAAC AGACCTTTTC TCAGTTTGAA GCATACCCCA
    ATGGGCAAGA AGTCTAGACG GCCTAACACC AAGAGTACCG TGCCCACTAG CCTCCCTAAC
    CTTGCCAAGG AGGCCAAGGA TGCCAAGGGG AACAGGAGGA AGAAGTCTCT GAGTGACAAG
    GGCCAGCTGT CCGAGAGCTC AGTGACTCTG TCCCCCGTCG ATTCCCTAGA ATCTCCGCAC
    ACATATGTTT CTGACACCAC CTCCTCTCCA ATGATCACAT CCCCCGGAAT CTTACAGGCC
    TCCCCCAACC CCATGCTGGC CACTGCTGCC CCCACTGCCC CAGTCCATGC ACAGCATGCA
    CTGTCTTTCT CTAACCTTCA TGAGATGCAG CCCTTGGCTC ATGGGGCCAA CACTGTGCTT
    CCCTCAGTGA GCCAGCTGCT GTCTCACCAC CACATTGTGC CCCCAGGCAG CAGCACCGCA
    GGGAGCTTGG GTAGGCTACA TTCAGTCACT GTCCCGGCCG ACTGGATGAA CCGCGTGGAG
    ATGAATGAGA GCCAGTACAA CGAGATGTTC GGTATGGTCC TGGCTCCAGC TGAGGGCACC
    CACCCTGGCA TAGCTCCCCA GAGCAGGCCG CCCGAAGGGA AGCATTTAAC CACCCCCCGA
    GAGCCCTTGC CCCCCATTGT GACTTTCCAG CTAATCCCCA AAGGCAGCAT TGCCCAACCA
    ACTGGGGCTG CCCAGCCTCC GTCCAACTGC CCTCCAGCCG TTGCAGGCAC CCTGCCCACC
    ATGTATCAGA TTCCAGAAAT GGCTCGCTTG CCCAGTATGG CTTTTCCCAC TGCCATGATG
    CCCCAGCAGG ACGGGCAGGT TGCTCAGACC ATTCTCCCAG CCTATCATGC CTTCCCAGCC
    TCTGTGGGCA AGTATCCCAC ACCCCCATCA CAGCATAGCT ATGCATCCTC CAATGCTGCT
    GAGAGAACGC CCAGTCACAG TGGTCACCTC CAGGGTGAGC ACCCCTACCT GACACCGTCC
    CCAGAGTCTC CTGACCAGTG GTCAAGTTCA TCACCCCACT CTGCTTCTGA CTGGTCAGAT
    GTGACCACCA GCCCTACTCC TGGGGGCGCT GGAGGAGGTC AGCGGGGACC TGGGACACAC
    ATGTCTGAGC CACCACGCAG CAACATGCAG GTCTATGCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006049410.1
    CDS247..7548
    Translation

    Target ORF information:

    RefSeq Version XM_006049348.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis notch 2 (NOTCH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006049348.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCCCGCCC TGCGCCCCGC TCCGCTGTGG GCGCTGCTGG CACTGTGGCT GTGCCGCGCG 
    GCCCCGGCGC GTGCATTGCA ATGTCGAGAT GGCTATGAGC CTTGTGTAAA TGAAGGAATA
    TGTGTTACCT ACCACAACGG TACAGGATAC TGCAAGTGTC CAGAGGGCTT CTTGGGAGAA
    TATTGTCAGC ATCGAGACCC CTGTGAGAAG AACCGTTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCTA TGTTGGGGAA AGCCACATGC CGCTGTGCCT CGGGCTTCAC GGGCGAGGAC
    TGCCAGCACT CGACTACTCA CCCCTGCTTT GTGTCTCACC CCTGCCTGAA TGGGGGCACC
    TGCCACGTGC TTAGCCGGGA CACCTACGAG TGCACCTGCC AAGTCGGTTT TACAGGTAAA
    CTGTGCCAGT GGACTGATGC CTGCCTGTCC CACCCCTGTG CAAACGGAAG TACCTGTACC
    ACTATGGCCA ACCAGTTCTC CTGCACATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG
    ACTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ACGGTGGCAC CTGCCTCAAC
    CTCCCGGGCT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCCAGCA CTGCGACAGT
    CCCTACGTGC CCTGCGCACC CTCCCCCTGT GTCAACGGAG GTACCTGCCG GCAAACCGGT
    GACTTTACCT TTGAGTGCAG TTGCCTTCCA GGTTTTGAAG GAGTCACCTG TGAGCGGAAT
    ATAGATGACT GTCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAT
    ACCTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGCCTGC TGCAACCCAA TGCCTGTCAG AACGGGGGCA CCTGTACCAA CCGCAACGGG
    GGCTATGGCT GTGTATGTGT GAATGGCTGG AGTGGGGACG ACTGCAGTGA GAATATCGAT
    GACTGTGCCT TCGCCTCCTG CACCCCGGGC TCTACTTGCA TCGATCGTGT GGCCTCCTTC
    TCTTGTATGT GCCCAGAAGG AAAGGCAGGT CTCTTATGTC ATCTGGATGA CGCGTGCATC
    AGCAACCCCT GCCACAAGGG GGCGCTGTGT GATACCAACC CTCTGAATGG ACAGTATATT
    TGCACCTGCC CACAAGGCTA CAAAGGGAGT GACTGTACAG AAGATGTGGA TGAGTGTGCC
    ATGGCCAACA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAGT GTCTGAAGGG CTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC
    CACTCAGACC CCTGCAAGAA TGATGCTACC TGTTTGGATA AGATTGGAGG CTTCACGTGT
    CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAGTTGG AGATAAATGA ATGTCAGAGC
    AACCCCTGTG TGAACAATGG ACAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTGTGT
    CCGCCTGGGT TCACTGGTGT GTTGTGTGAG GAGAACATTG ACAACTGTGA CCCTGATCCT
    TGCCACCACG GCCAGTGTCA GGACGGCATT GATTCCTACA CCTGCATCTG CAGTCCGGGC
    TACATGGGCG CCATCTGCAG TGACCAGATA GACGAATGCT ACAGCAGCCC TTGCCTGAAT
    GAGGGTCGCT GCATCGACCT GGTGAATGGT TACCAGTGCA ACTGCCAGCC AGGCACGTCA
    GGTGTTAATT GTGAAATCAA TTTTGATGAC TGTGCAAGTA ACCCGTGTGT CCATGGAGTC
    TGTATGGATG GCGTTAATCG TTACAGCTGT GTCTGCTCAC CAGGATTCAC AGGGCAGAGA
    TGCAACATTG ACATTGATGA GTGTGCCTCC AATCCCTGTC GCAAGGGTGC AACATGCATC
    AATGATGTGA ACGGTTTCCG CTGTATCTGC CCTGAGGGCC CCCACCATCC CAGCTGCTAC
    TCACAGGTGA ACGAGTGCCT GAGCAATCCC TGTGTCCACG GAAACTGTAC TGGGGGCCTC
    ACTGGATACA AGTGCCTCTG TGATGCAGGC TGGGTTGGCA TCAACTGTGA AGTGGACAAA
    AATGAATGTC TTTCTAATCC ATGCCAGAAC GGGGGAACTT GTGACAATCT GGTGAATGGA
    TACAGGTGCA CTTGCAAGAA GGGCTTTAAA GGCTATAACT GCCAGGTGAA TATTGATGAA
    TGTGCTTCAA ATCCATGTCT GAACCAAGGA ACCTGCCTTG ATGACGTAAG CGGTTATACT
    TGCCACTGTG TGCTGCCATA CACAGGCAAG AACTGTCAGA CCGTATTGGC TCCCTGTTCC
    CCAAACCCTT GTGAGAATGC TGGTGTTTGC AAAGAGGCAC CAAATTTTGA GAGTTACTCC
    TGCCTGTGTG CTCCTGGCTG GAAAGGTCAG CGGTGTACCA TCGACATTGA TGAGTGTGTC
    TCCAAGCCCT GCATGAACCA CGGTCTCTGC CATAACACCC AGGGCAGCTA CATGTGTGAA
    TGTCCTCCAG GCTTCAGTGG CATGGACTGT GAGGAGGACG TTGATGACTG CCTTGCCAAT
    CCTTGCCAGA ATGGAGGTTC CTGTGTAGAT GGAGTGAATA CTTTCTCCTG CCTGTGCCTT
    CCGGGCTTCA CTGGGGATAA ATGTCAGACA GACATGAATG AGTGTCTGAG TGAGCCCTGT
    AAGAACGGAG GGACCTGCTC TGACTATGTC AACAGTTACA CCTGCAAGTG CCCGGCGGGA
    TTTGATGGAG TCCACTGTGA GAACAACATT GACGAGTGCA CTGAGAGCTC CTGTTTCAAT
    GGTGGCACGT GTATCGATGG GATTAACTCC TTCTCTTGCT TGTGCCCTGT GGGCTTCACC
    GGTTCCTTCT GCCTCCATGA GATCAATGAA TGCAACTCTC ATCCATGCCT GAATGAAGGA
    GTATGTGTTG ATGGCCTGGG TACCTACCGC TGTATCTGCC CATTGGGCTA CACTGGAAAA
    AACTGTCAGA CCCTGGTGAA CCTCTGTAGT CGGTCTCCAT GTAAAAACAA AGGTACCTGC
    ATTCAGGATA AAGCAGAGTC ACGGTGCCTG TGTCCGTCTG GATGGGCTGG TGCTTACTGC
    GATGTTCCCA GTGTCTCCTG TGAGGTGGCG GCCTCCCACA GAGGGGTGCC CGTTGACCGC
    TTGTGCCAGC ACTCAGGTGT CTGTATCAGT GCGGGCAACT CGCATCACTG CCAGTGCCCC
    TTGGGCTACA CCGGGAGCTA CTGTGAGGAC CAGCTTGATG AGTGCTCGTC CAATCCCTGC
    CAGCACGGCG CCACCTGCCG GGACTTCATT GGTGGATACA GATGTGAGTG TGTCCCTGGA
    TATCAGGGTG TCAACTGCGA GTATGAAGTG GATGAGTGCC AGAACCAGCC ATGCCAGAAT
    GGGGGCACCT GTATCGACCT TGTGAACCAC TTCAAGTGCT CCTGCCCACC AGGCACTCGG
    GGCCTGCTTT GTGAAGAGAA CGTTGACGAC TGTGCCAGGG GTCCCCACTG CCTTAATGGT
    GGCCAGTGTG TAGACAGGAT TGGGGGCTAC AGTTGTCGCT GCTTGCCAGG CTTTGCTGGA
    GAACGCTGTG AGGGGGACAT CAACGAGTGC CTGTCCAACC CCTGCAGCTC TGAGGGCAGC
    CTGGACTGCA TCCAGCTCAC CAACGACTAC CTGTGCGTCT GCCGCAGCAC CTTCACTGGT
    CGTCACTGTG AAACGTTCGT CGACGTGTGT CCCCAGATGC CTTGCCTGAA CGGAGGCACT
    TGTGCTGTGG CCAGCAACAT GCCCGATGGT TTCATCTGTC GTTGTCCCCC GGGCTTCTCT
    GGGGCGCGGT GCCAGAGCAG CTGCGGACAG GTGAAGTGCA GGAGAGGGGA GCAGTGTGTG
    CACACAGCCT CGGGCCCCCG CTGCTTCTGT CCCAACCCCC AGGACTGCGA CTCGGGCTGC
    GCCAGTAGCC CCTGCCAGCA CGGGGGGAGC TGCCGCCCTC AGCGCCAGCC TCCTTACTAC
    TCGTGCCAGT GTGCCCCGCC GTTCCGGGGC AGCCACTGTG AACTCTACAC CGCCCCCACC
    AGCACCCCTC CCGCCACCTG CCTGAGCCAG TACTGTGCCG ACAAAGCTCG GGACGGCATC
    TGTGATGACG TGTGCAACAG CCATGCCTGC CAGTGGGATG GGGGGGACTG TTCCCTCACC
    ATGGAGGACC CCTGGGCCAA TTGCTCCTCC CCGCTGCCCT GCTGGGAGTA CATCAACAAC
    CAGTGCGACG AGCTGTGCAA CACAGCCGAG TGCCTGTTTG ACAACTTTGA ATGCCAGAAG
    AGTAGCAAGA CATGCAAATA TGACAAATAC TGTGCAGACC ACTACGAAGA CAACCATTGT
    GACCAGGGTT GCAACAGTGA GGAGTGTGGC TGGGATGGGC TGGACTGTGC TGCTGACCGG
    CCAGAAAACC TGGCAGAGGG CACCCTGGTC ATCGTGGTGC TGATGCCACC TGAACAACTG
    CTCCAGGATG CCCGCAGATT CTTGCGGGCA CTGGGCACCC TGCTCCACAC CAACCTCCGC
    ATCAAACGGG ATGCCCAGGG GGAACTCATG GTCTACCCCT ATTATGGTGG GAAGTCAGCT
    GCCATGAAGA AGCAGAGATT GACACGCAGG TCCCTTCCTG GAGATGAAGA ACAGGAAGTG
    GCTGGCTCTC AGGTATTTCT GGAGATTGAC AACCGCCAGT GTGTTCAAGA CTCAGACCAG
    TGCTTTAAGA ACACAGATGC AGCCGCAGCT CTTCTGGCTT CTCACGCCAT CCAGGGGACC
    CTGTCGTACC CACTTGTGTC TGTCGTCAGT GAGTCTCTGA CCCACAAACG CACTCAGCTT
    CTCTATCTGC TTGCTGTTGC CGTTGTCATT ATCTTGTTTA TTATCCTGCT GGGGGTTATC
    ATGGCAAAAC GAAAGCGCAA GCATGGCTCC CTCTGGCTGC CTGAAGGTTT CACCCTTCGC
    CGTGACTCCA GCAACCACAA ACGTCGTGAG CCAGTGGGCC AGGATGCTGT GGGACTCAAA
    AATCTCTCAG TGCAAGTCTC AGAGGCGAAC CTAATTGGTT CTGGAACAAG TGAACACTGG
    GTCGACGACG AAGGGCCGCA GCCTAAGAAA GCCAAGGCTG AAGATGAGGC TTTGCTCTCA
    GAAGAAGATG ACCCCATCGA TCGGCGTCCG TGGACACAGC AGCACCTGGA AGCTGCAGAC
    ATCCGTAGGA CACCATCGCT GGCTCTTACC CCTCCACAGG CAGAGCAGGA GGTGGACGTG
    CTGGATGTGA ACGTCCGTGG TCCAGATGGG TGCACCCCAC TGATGCTGGC TTCTCTCCGA
    GGAGGCAGCT CAGACATGAG TGATGAAGAT GAAGACACAG AGGACTCTTC TGCCAACATC
    ATCACGGACT TAGTCTATCA GGGTGCCAGC CTCCAAGCAC AGACAGACCG GACGGGTGAG
    ATGGCCCTGC ATCTCGCAGC CCGGTACTCA CGGGCAGATG CTGCCAAGCG TCTCCTGGAT
    GCAGGTGCAG ATGCCAATGC CCAGGACAAC ATGGGCCGCT GCCCTCTCCA TGCTGCAGTG
    GCCGCTGATG CCCAAGGTGT CTTCCAGATA CTGATCCGCA ACCGAGTAAC TGATCTAGAC
    GCAAGGATGA ATGATGGCAC TACACCCCTG ATCCTGGCTG CCCGCCTGGC AGTGGAGGGA
    ATGGTGGCAG AACTGATCAA CTGCCAAGCA GATGTGAATG CAGTGGATGA CCATGGAAAA
    TCTGCCCTTC ACTGGGCAGC TGCTGTGAAT AATGTGGAGG CAACCCTTTT GCTGTTGAAA
    AATGGGGCCA ACCGAGACAT GCAGGACAAC AAGGAGGAGA CACCCCTGTT TCTTGCTGCT
    CGGGAGGGGA GCTATGAAGC AGCCAAGATC CTGTTAGACC ATTTTGCCAA TCGGGACATC
    ACAGACCACA TGGATCGCCT TCCCCGGGAC GTAGCCCGGG ACCGCATGCA CCATGACATC
    GTGCGCCTGC TGGACGAGTA CAACGTGACC CCCAGCCCTC CCGGCACAGT GCTGACGTCT
    GCGCTCTCAC CCGTCATCTG TGGGCCCAAC AGACCTTTTC TCAGTTTGAA GCATACCCCA
    ATGGGCAAGA AGTCTAGACG GCCTAACACC AAGAGTACCG TGCCCACTAG CCTCCCTAAC
    CTTGCCAAGG AGGCCAAGGA TGCCAAGGGG AACAGGAGGA AGAAGTCTCT GAGTGACAAG
    GGCCAGCTGT CCGAGAGCTC AGTGACTCTG TCCCCCGTCG ATTCCCTAGA ATCTCCGCAC
    ACATATGTTT CTGACACCAC CTCCTCTCCA ATGATCACAT CCCCCGGAAT CTTACAGGCC
    TCCCCCAACC CCATGCTGGC CACTGCTGCC CCCACTGCCC CAGTCCATGC ACAGCATGCA
    CTGTCTTTCT CTAACCTTCA TGAGATGCAG CCCTTGGCTC ATGGGGCCAA CACTGTGCTT
    CCCTCAGTGA GCCAGCTGCT GTCTCACCAC CACATTGTGC CCCCAGGCAG CAGCACCGCA
    GGGAGCTTGG GTAGGCTACA TTCAGTCACT GTCCCGGCCG ACTGGATGAA CCGCGTGGAG
    ATGAATGAGA GCCAGTACAA CGAGATGTTC GGTATGGTCC TGGCTCCAGC TGAGGGCACC
    CACCCTGGCA TAGCTCCCCA GAGCAGGCCG CCCGAAGGGA AGCATTTAAC CACCCCCCGA
    GAGCCCTTGC CCCCCATTGT GACTTTCCAG CTAATCCCCA AAGGCAGCAT TGCCCAACCA
    ACTGGGGCTG CCCAGCCTCC GTCCAACTGC CCTCCAGCCG TTGCAGGCAC CCTGCCCACC
    ATGTATCAGA TTCCAGAAAT GGCTCGCTTG CCCAGTATGG CTTTTCCCAC TGCCATGATG
    CCCCAGCAGG ACGGGCAGGT TGCTCAGACC ATTCTCCCAG CCTATCATGC CTTCCCAGCC
    TCTGTGGGCA AGTATCCCAC ACCCCCATCA CAGCATAGCT ATGCATCCTC CAATGCTGCT
    GAGAGAACGC CCAGTCACAG TGGTCACCTC CAGGGTGAGC ACCCCTACCT GACACCGTCC
    CCAGAGTCTC CTGACCAGTG GTCAAGTTCA TCACCCCACT CTGCTTCTGA CTGGTCAGAT
    GTGACCACCA GCCCTACTCC TGGGGGCGCT GGAGGAGGTC AGCGGGGACC TGGGACACAC
    ATGTCTGAGC CACCACGCAG CAACATGCAG GTCTATGCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68174
    Clone ID Related Accession (Same CDS sequence) XM_006049346.2
    Accession Version XM_006049346.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product LOW QUALITY PROTEIN: neurogenic locus notch homolog protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006049346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCCCGCCC TGCGCCCCGC TCCGCTGTGG GCGCTGCTGG CACTGTGGCT GTGCCGCGCG 
    GCCCCGGCGC GTGCATTGCA ATGTCGAGAT GGCTATGAGC CTTGTGTAAA TGAAGGAATA
    TGTGTTACCT ACCACAACGG TACAGGATAC TGCAAGTGTC CAGAGGGCTT CTTGGGAGAA
    TATTGTCAGC ATCGAGACCC CTGTGAGAAG AACCGTTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCTA TGTTGGGGAA AGCCACATGC CGCTGTGCCT CGGGCTTCAC GGGCGAGGAC
    TGCCAGCACT CGACTACTCA CCCCTGCTTT GTGTCTCACC CCTGCCTGAA TGGGGGCACC
    TGCCACGTGC TTAGCCGGGA CACCTACGAG TGCACCTGCC AAGTCGGTTT TACAGGTAAA
    CTGTGCCAGT GGACTGATGC CTGCCTGTCC CACCCCTGTG CAAACGGAAG TACCTGTACC
    ACTATGGCCA ACCAGTTCTC CTGCACATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG
    ACTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ACGGTGGCAC CTGCCTCAAC
    CTCCCGGGCT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCCAGCA CTGCGACAGT
    CCCTACGTGC CCTGCGCACC CTCCCCCTGT GTCAACGGAG GTACCTGCCG GCAAACCGGT
    GACTTTACCT TTGAGTGCAG TTGCCTTCCA GGTTTTGAAG GAGTCACCTG TGAGCGGAAT
    ATAGATGACT GTCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAT
    ACCTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGCCTGC TGCAACCCAA TGCCTGTCAG AACGGGGGCA CCTGTACCAA CCGCAACGGG
    GGCTATGGCT GTGTATGTGT GAATGGCTGG AGTGGGGACG ACTGCAGTGA GAATATCGAT
    GACTGTGCCT TCGCCTCCTG CACCCCGGGC TCTACTTGCA TCGATCGTGT GGCCTCCTTC
    TCTTGTATGT GCCCAGAAGG AAAGGCAGGT CTCTTATGTC ATCTGGATGA CGCGTGCATC
    AGCAACCCCT GCCACAAGGG GGCGCTGTGT GATACCAACC CTCTGAATGG ACAGTATATT
    TGCACCTGCC CACAAGGCTA CAAAGGGAGT GACTGTACAG AAGATGTGGA TGAGTGTGCC
    ATGGCCAACA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAGT GTCTGAAGGG CTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC
    CACTCAGACC CCTGCAAGAA TGATGCTACC TGTTTGGATA AGATTGGAGG CTTCACGTGT
    CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAGTTGG AGATAAATGA ATGTCAGAGC
    AACCCCTGTG TGAACAATGG ACAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTGTGT
    CCGCCTGGAT TCACGGGGCC AGTTTGCCAG ATCGATATTG ACGACTGTTC TAGTACTCCG
    TGTCTGAATG GGGCAAAGTG TATAGATCAC CCGAATGGCT ATGAGTGCCA GTGTGCCACA
    GGGTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC
    CACGGCCAGT GTCAGGACGG CATTGATTCC TACACCTGCA TCTGCAGTCC GGGCTACATG
    GGCGCCATCT GCAGTGACCA GATAGACGAA TGCTACAGCA GCCCTTGCCT GAATGAGGGT
    CGCTGCATCG ACCTGGTGAA TGGTTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGTGTT
    AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCGT GTGTCCATGG AGTCTGTATG
    GATGGCGTTA ATCGTTACAG CTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGCAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG CATCAATGAT
    GTGAACGGTT TCCGCTGTAT CTGCCCTGAG GGCCCCCACC ATCCCAGCTG CTACTCACAG
    GTGAACGAGT GCCTGAGCAA TCCCTGTGTC CACGGAAACT GTACTGGGGG CCTCACTGGA
    TACAAGTGCC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA
    TGTCTTTCTA ATCCATGCCA GAACGGGGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGCACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCT
    TCAAATCCAT GTCTGAACCA AGGAACCTGC CTTGATGACG TAAGCGGTTA TACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAACTGT CAGACCGTAT TGGCTCCCTG TTCCCCAAAC
    CCTTGTGAGA ATGCTGGTGT TTGCAAAGAG GCACCAAATT TTGAGAGTTA CTCCTGCCTG
    TGTGCTCCTG GCTGGAAAGG TCAGCGGTGT ACCATCGACA TTGATGAGTG TGTCTCCAAG
    CCCTGCATGA ACCACGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCT
    CCAGGCTTCA GTGGCATGGA CTGTGAGGAG GACGTTGATG ACTGCCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG CCTTCCGGGC
    TTCACTGGGG ATAAATGTCA GACAGACATG AATGAGTGTC TGAGTGAGCC CTGTAAGAAC
    GGAGGGACCT GCTCTGACTA TGTCAACAGT TACACCTGCA AGTGCCCGGC GGGATTTGAT
    GGAGTCCACT GTGAGAACAA CATTGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACGTGTATCG ATGGANTTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGCTT CACCGGTTCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCATCCAT GCCTGAATGA AGGAGTATGT
    GTTGATGGCC TGGGTACCTA CCGCTGTATC TGCCCATTGG GCTACACTGG AAAAAACTGT
    CAGACCCTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC CTGCATTCAG
    GATAAAGCAG AGTCACGGTG CCTGTGTCCG TCTGGATGGG CTGGTGCTTA CTGCGATGTT
    CCCAGTGTCT CCTGTGAGGT GGCGGCCTCC CACAGAGGGG TGCCCGTTGA CCGCTTGTGC
    CAGCACTCAG GTGTCTGTAT CAGTGCGGGC AACTCGCATC ACTGCCAGTG CCCCTTGGGC
    TACACCGGGA GCTACTGTGA GGACCAGCTT GATGAGTGCT CGTCCAATCC CTGCCAGCAC
    GGCGCCACCT GCCGGGACTT CATTGGTGGA TACAGATGTG AGTGTGTCCC TGGATATCAG
    GGTGTCAACT GCGAGTATGA AGTGGATGAG TGCCAGAACC AGCCATGCCA GAATGGGGGC
    ACCTGTATCG ACCTTGTGAA CCACTTCAAG TGCTCCTGCC CACCAGGCAC TCGGGGCCTG
    CTTTGTGAAG AGAACGTTGA CGACTGTGCC AGGGGTCCCC ACTGCCTTAA TGGTGGCCAG
    TGTGTAGACA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CAGGCTTTGC TGGAGAACGC
    TGTGAGGGGG ACATCAACGA GTGCCTGTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGCATCCAGC TCACCAACGA CTACCTGTGC GTCTGCCGCA GCACCTTCAC TGGTCGTCAC
    TGTGAAACGT TCGTCGACGT GTGTCCCCAG ATGCCTTGCC TGAACGGAGG CACTTGTGCT
    GTGGCCAGCA ACATGCCCGA TGGTTTCATC TGTCGTTGTC CCCCGGGCTT CTCTGGGGCG
    CGGTGCCAGA GCAGCTGCGG ACAGGTGAAG TGCAGGAGAG GGGAGCAGTG TGTGCACACA
    GCCTCGGGCC CCCGCTGCTT CTGTCCCAAC CCCCAGGACT GCGACTCGGG CTGCGCCAGT
    AGCCCCTGCC AGCACGGGGG GAGCTGCCGC CCTCAGCGCC AGCCTCCTTA CTACTCGTGC
    CAGTGTGCCC CGCCGTTCCG GGGCAGCCAC TGTGAACTCT ACACCGCCCC CACCAGCACC
    CCTCCCGCCA CCTGCCTGAG CCAGTACTGT GCCGACAAAG CTCGGGACGG CATCTGTGAT
    GACGTGTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGGG ACTGTTCCCT CACCATGGAG
    GACCCCTGGG CCAATTGCTC CTCCCCGCTG CCCTGCTGGG AGTACATCAA CAACCAGTGC
    GACGAGCTGT GCAACACAGC CGAGTGCCTG TTTGACAACT TTGAATGCCA GAAGAGTAGC
    AAGACATGCA AATATGACAA ATACTGTGCA GACCACTACG AAGACAACCA TTGTGACCAG
    GGTTGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAA
    AACCTGGCAG AGGGCACCCT GGTCATCGTG GTGCTGATGC CACCTGAACA ACTGCTCCAG
    GATGCCCGCA GATTCTTGCG GGCACTGGGC ACCCTGCTCC ACACCAACCT CCGCATCAAA
    CGGGATGCCC AGGGGGAACT CATGGTCTAC CCCTATTATG GTGGGAAGTC AGCTGCCATG
    AAGAAGCAGA GATTGACACG CAGGTCCCTT CCTGGAGATG AAGAACAGGA AGTGGCTGGC
    TCTCAGGTAT TTCTGGAGAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCAGTGCTTT
    AAGAACACAG ATGCAGCCGC AGCTCTTCTG GCTTCTCACG CCATCCAGGG CACCCTGTCG
    TACCCACTTG TGTCTGTCGT CAGTGAGTCT CTGACCCACA AACGCACTCA GCTTCTCTAT
    CTGCTTGCTG TTGCCGTTGT CATTATCTTG TTTATTATCC TGCTGGGGGT TATCATGGCA
    AAACGAAAGC GCAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGTGAC
    TCCAGCAACC ACAAACGTCG TGAGCCAGTG GGCCAGGATG CTGTGGGACT CAAAAATCTC
    TCAGTGCAAG TCTCAGAGGC GAACCTAATT GGTTCTGGAA CAAGTGAACA CTGGGTCGAC
    GACGAAGGGC CACAGCCTAA GAAAGCCAAG GCTGAAGATG AGGCTTTGCT CTCAGAAGAA
    GATGACCCCA TCGATCGGCG TCCGTGGACA CAGCAGCACC TGGAAGCTGC AGACATCCGT
    AGGACACCAT CGCTGGCTCT TACCCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT
    GTGAACGTCC GTGGTCCAGA TGGGTGCACC CCACTGATGC TGGCTTCTCT CCGAGGAGGC
    AGCTCAGACA TGAGTGATGA AGATGAAGAC ACAGAGGACT CTTCTGCCAA CATCATCACG
    GACTTAGTCT ATCAGGGTGC CAGCCTCCAA GCACAGACAG ACCGGACGGG TGAGATGGCC
    CTGCATCTCG CAGCCCGGTA CTCACGGGCA GATGCTGCCA AGCGTCTCCT GGATGCAGGT
    GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGCCCTC TCCATGCTGC AGTGGCCGCT
    GATGCCCAAG GTGTCTTCCA GATACTGATC CGCAACCGAG TAACTGATCT AGACGCAAGG
    ATGAATGATG GCACTACACC CCTGATCCTG GCTGCCCGCC TGGCAGTGGA GGGAATGGTG
    GCAGAACTGA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCC
    CTTCACTGGG CAGCTGCTGT GAATAATGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAGGA CAACAAGGAG GAGACACCCC TGTTTCTTGC TGCTCGGGAG
    GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGGGA CATCACAGAC
    CACATGGATC GCCTTCCCCG GGACGTAGCC CGGGACCGCA TGCACCATGA CATCGTGCGC
    CTGCTGGACG AGTACAACGT GACCCCCAGC CCTCCCGGCA CAGTGCTGAC GTCTGCGCTC
    TCACCCGTCA TCTGTGGGCC CAACAGACCT TTTCTCAGTT TGAAGCATAC CCCAATGGGC
    AAGAAGTCTA GACGGCCTAA CACCAAGAGT ACCGTGCCCA CTAGCCTCCC TAACCTTGCC
    AAGGAGGCCA AGGATGCCAA GGGGAACAGG AGGAAGAAGT CTCTGAGTGA CAAGGGCCAG
    CTGTCCGAGA GCTCAGTGAC TCTGTCCCCC GTCGATTCCC TAGAATCTCC GCACACATAT
    GTTTCTGACA CCACCTCCTC TCCAATGATC ACATCCCCCG GAATCTTACA GGCCTCCCCC
    AACCCCATGC TGGCCACTGC TGCCCCCACT GCCCCAGTCC ATGCACAGCA TGCACTGTCT
    TTCTCTAACC TTCATGAGAT GCAGCCCTTG GCTCATGGGG CCAACACTGT GCTTCCCTCA
    GTGAGCCAGC TGCTGTCTCA CCACCACATT GTGCCCCCAG GCAGCAGCAC CGCAGGGAGC
    TTGGGTAGGC TACATTCAGT CACTGTCCCG GCCGACTGGA TGAACCGCGT GGAGATGAAT
    GAGAGCCAGT ACAACGAGAT GTTCGGTATG GTCCTGGCTC CAGCTGAGGG CACCCACCCT
    GGCATAGCTC CCCAGAGCAG GCCGCCCGAA GGGAAGCATT TAACCACCCC CCGAGAGCCC
    TTGCCCCCCA TTGTGACTTT CCAGCTAATC CCCAAAGGCA GCATTGCCCA ACCAACTGGG
    GCTGCCCAGC CTCCGTCCAA CTGCCCTCCA GCCGTTGCAG GCACCCTGCC CACCATGTAT
    CAGATTCCAG AAATGGCTCG CTTGCCCAGT ATGGCTTTTC CCACTGCCAT GATGCCCCAG
    CAGGACGGGC AGGTTGCTCA GACCATTCTC CCAGCCTATC ACGCCTTCCC AGCCTCTGTG
    GGCAAGTATC CCACACCCCC ATCACAGCAT AGCTATGCAT CCTCCAATGC TGCTGAGAGA
    ACGCCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCACCCCT ACCTGACACC GTCCCCAGAG
    TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC
    ACCAGCCCTA CTCCTGGGGG CGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT
    GAGCCACCAC GCAGCAACAT GCAGGTCTAT GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006049408.2
    CDS255..7670
    Translation

    Target ORF information:

    RefSeq Version XM_006049346.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis notch 2 (NOTCH2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006049346.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCCCGCCC TGCGCCCCGC TCCGCTGTGG GCGCTGCTGG CACTGTGGCT GTGCCGCGCG 
    GCCCCGGCGC GTGCATTGCA ATGTCGAGAT GGCTATGAGC CTTGTGTAAA TGAAGGAATA
    TGTGTTACCT ACCACAACGG TACAGGATAC TGCAAGTGTC CAGAGGGCTT CTTGGGAGAA
    TATTGTCAGC ATCGAGACCC CTGTGAGAAG AACCGTTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCTA TGTTGGGGAA AGCCACATGC CGCTGTGCCT CGGGCTTCAC GGGCGAGGAC
    TGCCAGCACT CGACTACTCA CCCCTGCTTT GTGTCTCACC CCTGCCTGAA TGGGGGCACC
    TGCCACGTGC TTAGCCGGGA CACCTACGAG TGCACCTGCC AAGTCGGTTT TACAGGTAAA
    CTGTGCCAGT GGACTGATGC CTGCCTGTCC CACCCCTGTG CAAACGGAAG TACCTGTACC
    ACTATGGCCA ACCAGTTCTC CTGCACATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG
    ACTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ACGGTGGCAC CTGCCTCAAC
    CTCCCGGGCT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCCAGCA CTGCGACAGT
    CCCTACGTGC CCTGCGCACC CTCCCCCTGT GTCAACGGAG GTACCTGCCG GCAAACCGGT
    GACTTTACCT TTGAGTGCAG TTGCCTTCCA GGTTTTGAAG GAGTCACCTG TGAGCGGAAT
    ATAGATGACT GTCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAT
    ACCTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGCCTGC TGCAACCCAA TGCCTGTCAG AACGGGGGCA CCTGTACCAA CCGCAACGGG
    GGCTATGGCT GTGTATGTGT GAATGGCTGG AGTGGGGACG ACTGCAGTGA GAATATCGAT
    GACTGTGCCT TCGCCTCCTG CACCCCGGGC TCTACTTGCA TCGATCGTGT GGCCTCCTTC
    TCTTGTATGT GCCCAGAAGG AAAGGCAGGT CTCTTATGTC ATCTGGATGA CGCGTGCATC
    AGCAACCCCT GCCACAAGGG GGCGCTGTGT GATACCAACC CTCTGAATGG ACAGTATATT
    TGCACCTGCC CACAAGGCTA CAAAGGGAGT GACTGTACAG AAGATGTGGA TGAGTGTGCC
    ATGGCCAACA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAGT GTCTGAAGGG CTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC
    CACTCAGACC CCTGCAAGAA TGATGCTACC TGTTTGGATA AGATTGGAGG CTTCACGTGT
    CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAGTTGG AGATAAATGA ATGTCAGAGC
    AACCCCTGTG TGAACAATGG ACAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTGTGT
    CCGCCTGGAT TCACGGGGCC AGTTTGCCAG ATCGATATTG ACGACTGTTC TAGTACTCCG
    TGTCTGAATG GGGCAAAGTG TATAGATCAC CCGAATGGCT ATGAGTGCCA GTGTGCCACA
    GGGTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC
    CACGGCCAGT GTCAGGACGG CATTGATTCC TACACCTGCA TCTGCAGTCC GGGCTACATG
    GGCGCCATCT GCAGTGACCA GATAGACGAA TGCTACAGCA GCCCTTGCCT GAATGAGGGT
    CGCTGCATCG ACCTGGTGAA TGGTTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGTGTT
    AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCGT GTGTCCATGG AGTCTGTATG
    GATGGCGTTA ATCGTTACAG CTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGCAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG CATCAATGAT
    GTGAACGGTT TCCGCTGTAT CTGCCCTGAG GGCCCCCACC ATCCCAGCTG CTACTCACAG
    GTGAACGAGT GCCTGAGCAA TCCCTGTGTC CACGGAAACT GTACTGGGGG CCTCACTGGA
    TACAAGTGCC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA
    TGTCTTTCTA ATCCATGCCA GAACGGGGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGCACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCT
    TCAAATCCAT GTCTGAACCA AGGAACCTGC CTTGATGACG TAAGCGGTTA TACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAACTGT CAGACCGTAT TGGCTCCCTG TTCCCCAAAC
    CCTTGTGAGA ATGCTGGTGT TTGCAAAGAG GCACCAAATT TTGAGAGTTA CTCCTGCCTG
    TGTGCTCCTG GCTGGAAAGG TCAGCGGTGT ACCATCGACA TTGATGAGTG TGTCTCCAAG
    CCCTGCATGA ACCACGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCT
    CCAGGCTTCA GTGGCATGGA CTGTGAGGAG GACGTTGATG ACTGCCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG CCTTCCGGGC
    TTCACTGGGG ATAAATGTCA GACAGACATG AATGAGTGTC TGAGTGAGCC CTGTAAGAAC
    GGAGGGACCT GCTCTGACTA TGTCAACAGT TACACCTGCA AGTGCCCGGC GGGATTTGAT
    GGAGTCCACT GTGAGAACAA CATTGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACGTGTATCG ATGGANTTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGCTT CACCGGTTCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCATCCAT GCCTGAATGA AGGAGTATGT
    GTTGATGGCC TGGGTACCTA CCGCTGTATC TGCCCATTGG GCTACACTGG AAAAAACTGT
    CAGACCCTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC CTGCATTCAG
    GATAAAGCAG AGTCACGGTG CCTGTGTCCG TCTGGATGGG CTGGTGCTTA CTGCGATGTT
    CCCAGTGTCT CCTGTGAGGT GGCGGCCTCC CACAGAGGGG TGCCCGTTGA CCGCTTGTGC
    CAGCACTCAG GTGTCTGTAT CAGTGCGGGC AACTCGCATC ACTGCCAGTG CCCCTTGGGC
    TACACCGGGA GCTACTGTGA GGACCAGCTT GATGAGTGCT CGTCCAATCC CTGCCAGCAC
    GGCGCCACCT GCCGGGACTT CATTGGTGGA TACAGATGTG AGTGTGTCCC TGGATATCAG
    GGTGTCAACT GCGAGTATGA AGTGGATGAG TGCCAGAACC AGCCATGCCA GAATGGGGGC
    ACCTGTATCG ACCTTGTGAA CCACTTCAAG TGCTCCTGCC CACCAGGCAC TCGGGGCCTG
    CTTTGTGAAG AGAACGTTGA CGACTGTGCC AGGGGTCCCC ACTGCCTTAA TGGTGGCCAG
    TGTGTAGACA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CAGGCTTTGC TGGAGAACGC
    TGTGAGGGGG ACATCAACGA GTGCCTGTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGCATCCAGC TCACCAACGA CTACCTGTGC GTCTGCCGCA GCACCTTCAC TGGTCGTCAC
    TGTGAAACGT TCGTCGACGT GTGTCCCCAG ATGCCTTGCC TGAACGGAGG CACTTGTGCT
    GTGGCCAGCA ACATGCCCGA TGGTTTCATC TGTCGTTGTC CCCCGGGCTT CTCTGGGGCG
    CGGTGCCAGA GCAGCTGCGG ACAGGTGAAG TGCAGGAGAG GGGAGCAGTG TGTGCACACA
    GCCTCGGGCC CCCGCTGCTT CTGTCCCAAC CCCCAGGACT GCGACTCGGG CTGCGCCAGT
    AGCCCCTGCC AGCACGGGGG GAGCTGCCGC CCTCAGCGCC AGCCTCCTTA CTACTCGTGC
    CAGTGTGCCC CGCCGTTCCG GGGCAGCCAC TGTGAACTCT ACACCGCCCC CACCAGCACC
    CCTCCCGCCA CCTGCCTGAG CCAGTACTGT GCCGACAAAG CTCGGGACGG CATCTGTGAT
    GACGTGTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGGG ACTGTTCCCT CACCATGGAG
    GACCCCTGGG CCAATTGCTC CTCCCCGCTG CCCTGCTGGG AGTACATCAA CAACCAGTGC
    GACGAGCTGT GCAACACAGC CGAGTGCCTG TTTGACAACT TTGAATGCCA GAAGAGTAGC
    AAGACATGCA AATATGACAA ATACTGTGCA GACCACTACG AAGACAACCA TTGTGACCAG
    GGTTGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAA
    AACCTGGCAG AGGGCACCCT GGTCATCGTG GTGCTGATGC CACCTGAACA ACTGCTCCAG
    GATGCCCGCA GATTCTTGCG GGCACTGGGC ACCCTGCTCC ACACCAACCT CCGCATCAAA
    CGGGATGCCC AGGGGGAACT CATGGTCTAC CCCTATTATG GTGGGAAGTC AGCTGCCATG
    AAGAAGCAGA GATTGACACG CAGGTCCCTT CCTGGAGATG AAGAACAGGA AGTGGCTGGC
    TCTCAGGTAT TTCTGGAGAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCAGTGCTTT
    AAGAACACAG ATGCAGCCGC AGCTCTTCTG GCTTCTCACG CCATCCAGGG CACCCTGTCG
    TACCCACTTG TGTCTGTCGT CAGTGAGTCT CTGACCCACA AACGCACTCA GCTTCTCTAT
    CTGCTTGCTG TTGCCGTTGT CATTATCTTG TTTATTATCC TGCTGGGGGT TATCATGGCA
    AAACGAAAGC GCAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGTGAC
    TCCAGCAACC ACAAACGTCG TGAGCCAGTG GGCCAGGATG CTGTGGGACT CAAAAATCTC
    TCAGTGCAAG TCTCAGAGGC GAACCTAATT GGTTCTGGAA CAAGTGAACA CTGGGTCGAC
    GACGAAGGGC CACAGCCTAA GAAAGCCAAG GCTGAAGATG AGGCTTTGCT CTCAGAAGAA
    GATGACCCCA TCGATCGGCG TCCGTGGACA CAGCAGCACC TGGAAGCTGC AGACATCCGT
    AGGACACCAT CGCTGGCTCT TACCCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT
    GTGAACGTCC GTGGTCCAGA TGGGTGCACC CCACTGATGC TGGCTTCTCT CCGAGGAGGC
    AGCTCAGACA TGAGTGATGA AGATGAAGAC ACAGAGGACT CTTCTGCCAA CATCATCACG
    GACTTAGTCT ATCAGGGTGC CAGCCTCCAA GCACAGACAG ACCGGACGGG TGAGATGGCC
    CTGCATCTCG CAGCCCGGTA CTCACGGGCA GATGCTGCCA AGCGTCTCCT GGATGCAGGT
    GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGCCCTC TCCATGCTGC AGTGGCCGCT
    GATGCCCAAG GTGTCTTCCA GATACTGATC CGCAACCGAG TAACTGATCT AGACGCAAGG
    ATGAATGATG GCACTACACC CCTGATCCTG GCTGCCCGCC TGGCAGTGGA GGGAATGGTG
    GCAGAACTGA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCC
    CTTCACTGGG CAGCTGCTGT GAATAATGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAGGA CAACAAGGAG GAGACACCCC TGTTTCTTGC TGCTCGGGAG
    GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGGGA CATCACAGAC
    CACATGGATC GCCTTCCCCG GGACGTAGCC CGGGACCGCA TGCACCATGA CATCGTGCGC
    CTGCTGGACG AGTACAACGT GACCCCCAGC CCTCCCGGCA CAGTGCTGAC GTCTGCGCTC
    TCACCCGTCA TCTGTGGGCC CAACAGACCT TTTCTCAGTT TGAAGCATAC CCCAATGGGC
    AAGAAGTCTA GACGGCCTAA CACCAAGAGT ACCGTGCCCA CTAGCCTCCC TAACCTTGCC
    AAGGAGGCCA AGGATGCCAA GGGGAACAGG AGGAAGAAGT CTCTGAGTGA CAAGGGCCAG
    CTGTCCGAGA GCTCAGTGAC TCTGTCCCCC GTCGATTCCC TAGAATCTCC GCACACATAT
    GTTTCTGACA CCACCTCCTC TCCAATGATC ACATCCCCCG GAATCTTACA GGCCTCCCCC
    AACCCCATGC TGGCCACTGC TGCCCCCACT GCCCCAGTCC ATGCACAGCA TGCACTGTCT
    TTCTCTAACC TTCATGAGAT GCAGCCCTTG GCTCATGGGG CCAACACTGT GCTTCCCTCA
    GTGAGCCAGC TGCTGTCTCA CCACCACATT GTGCCCCCAG GCAGCAGCAC CGCAGGGAGC
    TTGGGTAGGC TACATTCAGT CACTGTCCCG GCCGACTGGA TGAACCGCGT GGAGATGAAT
    GAGAGCCAGT ACAACGAGAT GTTCGGTATG GTCCTGGCTC CAGCTGAGGG CACCCACCCT
    GGCATAGCTC CCCAGAGCAG GCCGCCCGAA GGGAAGCATT TAACCACCCC CCGAGAGCCC
    TTGCCCCCCA TTGTGACTTT CCAGCTAATC CCCAAAGGCA GCATTGCCCA ACCAACTGGG
    GCTGCCCAGC CTCCGTCCAA CTGCCCTCCA GCCGTTGCAG GCACCCTGCC CACCATGTAT
    CAGATTCCAG AAATGGCTCG CTTGCCCAGT ATGGCTTTTC CCACTGCCAT GATGCCCCAG
    CAGGACGGGC AGGTTGCTCA GACCATTCTC CCAGCCTATC ACGCCTTCCC AGCCTCTGTG
    GGCAAGTATC CCACACCCCC ATCACAGCAT AGCTATGCAT CCTCCAATGC TGCTGAGAGA
    ACGCCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCACCCCT ACCTGACACC GTCCCCAGAG
    TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC
    ACCAGCCCTA CTCCTGGGGG CGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT
    GAGCCACCAC GCAGCAACAT GCAGGTCTAT GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.