NOS3 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following NOS3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu02475 XM_006043055.1
Latest version!
Bubalus bubalis nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBu02476 XM_006043056.2
Latest version!
Bubalus bubalis nitric oxide synthase 3 (NOS3), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
OBu02476 XM_006043056.1 Bubalus bubalis nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu02475
    Clone ID Related Accession (Same CDS sequence) XM_006043055.1
    Accession Version XM_006043055.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product nitric oxide synthase, endothelial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783115.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGCGGCCT GGGGCTGGGG 
    CTGGGCCTCG GGCTATGCGG CAAGCAGGGC CCAGCCTCCC CGGCACCTGA GCCCAGCCGG
    GCCCCCGCAC CCGCCACCCC GCACGCACCA GACCACAGCC CAGCTCCCAA CAGCCCCACG
    CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGAGTGA AGAACTGGGA GCTGGGGAGC
    ATCACCTACG ACACTCTGTG CGCGCAGTCC CAACAGGACG GGCCCTGCAC TCCCAGACGC
    TGCCTGGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACCC GGCCCTCCCC GGGACCTCCA
    CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC
    AAGAGGAGCG GCTCCCAGGC TCACGAAGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA
    TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGCAACGCAC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTCGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGCG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CACAGCGCGC CCCGGGCCGT
    GGAGACTTCC GGATCTGGAA CAGCCAGCTG GTGCGCTACG CGGGCTACAG ACAGCAGGAT
    GGCTCTGTGC GTGGGGACCC AGCCAACGTG GAGATCACAG AGCTCTGCAT CCAGCACGGC
    TGGACCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGACGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTTG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCAGC CCTGGGCCTG CGCTGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC TCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACACG GAACTTGTGT GACCCTCACC GCTACAATAT CCTGGAGGAT
    GTGGCCGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACAGT GTCCTTCATG AAGCACCTGG ACAACGAGCA GAAGGCCAGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCGGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAACTACATC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAGG GGAGCGCAAC CAAGGGTGCC GGCATCACCA GAAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGTTATGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTCGATCCC CGGGTCCTGT GCATGGATGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGC GGCCAGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG CGTCTCCTGC
    TCAGACCCAC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGATAGC
    GCAGGGGCCC TGGGGACCCT CAGGTTCTGT GTGTTCGGAC TGGGCTCCCG GGCGTACCCC
    CACTTCTGCG CCTTCGCGCG AGCGGTGGAC ACCCGGCTGG AAGAGCTGGG AGGGGAGCGG
    CTGCTGCAGC TGGGCCAGGG CGACGAGCTC TGCGGCCAGG AGGAGGCCTT CCGTGGCTGG
    GCAAAGGCGG CCTTCCAGGC CTCCTGCGAG ACATTCTGTG TTGGGGAGGA GGCCAAGGCT
    GCCGCCCAGG ACATCTTCAG CCCCAAAAGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    GCCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG
    TTTCAGGCCA CAGTCCTCTC GGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACC
    ATCCTCGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC GGGGGACCAC
    ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTGGTGGAGG CGCTGCTGAG CCGCGTGGAG
    GACCCGCCAC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAAGG CAGCCCAGGC
    GGCCCTCCTC CCAGCTGGGT GCGGGACCCA CGGCTGCCCC CGTGCACACT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACCTCCCCG CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC
    ACCCTGGCCG AAGAACCCAG CGAGCAGCAG GAGCTCGAGA CCCTCAGTCA GGACCCCCGG
    CGCTATGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC
    CCGTCCGTGG CGCTGCCTGC CCCGCTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG
    TACTATTCCG TCAGCTCGGC CCCCAATGCC CACCCCGGAG AGGTCCACCT CACAGTGGCC
    GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACA
    TGGCTGAGCC AGCTCAAGAC TGGAGACCCC GTGCCCTGCT TCATCAGGGG GGCTCCCTCC
    TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCTGG CACTGGCATC
    GCCCCCTTCC GGGGATTTTG GCAGGAGAGG CTACATGACA TTGAGAGCAA AGGGCTGCAG
    CCCGCCCCCA TGACCCTGGT GTTCGGCTGC CGGTGCTCCC AACTCGACCA TCTCTACCGC
    GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC
    CGGGAACCTG ACAGCCCCAA GACTTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGACGTCACT
    ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGCATCTTGG CGACAGAGGG CGACATGGAG
    CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGTGC GGAAGGGCGG GGGCGCGGCC
    GGCGGGGACA GCCCCGGACG GGGGCGGGGC CTACCGCCGC TCTACACTTC CACCGGGGTT
    TTGATCCTCC GCGCTCTTTC CCTCCCAGGA TCAGCAACGC TATCACGAGG ACATTTTCGG
    CCTCACGCTG CGCACCCAGG AGGTGACAAG CCGTATACGT ACCCAGAGCT TTTCCCTGCA
    GGAACGGCAT CTGCGGGGCG CGGTGCCCTG GGCCTTCGAC CCGCCCGGCC CAGACACCCC
    CGGCCCCTGA GACCCCTCTT GCTTCCCACT GCAGTTCCCG GAGAGAGGGG CTGTCATTCC
    ACTATGGCTC TTCCGCTGTC CTGTCGGCCT TTACCAGGAC CGGCCACCTC TCCCTCTCCT
    CCCAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006043117.1
    CDS36..3884
    Translation

    Target ORF information:

    RefSeq Version XM_006043055.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006043055.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGCGGCCT GGGGCTGGGG 
    CTGGGCCTCG GGCTATGCGG CAAGCAGGGC CCAGCCTCCC CGGCACCTGA GCCCAGCCGG
    GCCCCCGCAC CCGCCACCCC GCACGCACCA GACCACAGCC CAGCTCCCAA CAGCCCCACG
    CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGAGTGA AGAACTGGGA GCTGGGGAGC
    ATCACCTACG ACACTCTGTG CGCGCAGTCC CAACAGGACG GGCCCTGCAC TCCCAGACGC
    TGCCTGGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACCC GGCCCTCCCC GGGACCTCCA
    CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC
    AAGAGGAGCG GCTCCCAGGC TCACGAAGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA
    TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGCAACGCAC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTCGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGCG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CACAGCGCGC CCCGGGCCGT
    GGAGACTTCC GGATCTGGAA CAGCCAGCTG GTGCGCTACG CGGGCTACAG ACAGCAGGAT
    GGCTCTGTGC GTGGGGACCC AGCCAACGTG GAGATCACAG AGCTCTGCAT CCAGCACGGC
    TGGACCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGACGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTTG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCAGC CCTGGGCCTG CGCTGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC TCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACACG GAACTTGTGT GACCCTCACC GCTACAATAT CCTGGAGGAT
    GTGGCCGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACAGT GTCCTTCATG AAGCACCTGG ACAACGAGCA GAAGGCCAGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCGGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAACTACATC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAGG GGAGCGCAAC CAAGGGTGCC GGCATCACCA GAAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGTTATGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTCGATCCC CGGGTCCTGT GCATGGATGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGC GGCCAGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG CGTCTCCTGC
    TCAGACCCAC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGATAGC
    GCAGGGGCCC TGGGGACCCT CAGGTTCTGT GTGTTCGGAC TGGGCTCCCG GGCGTACCCC
    CACTTCTGCG CCTTCGCGCG AGCGGTGGAC ACCCGGCTGG AAGAGCTGGG AGGGGAGCGG
    CTGCTGCAGC TGGGCCAGGG CGACGAGCTC TGCGGCCAGG AGGAGGCCTT CCGTGGCTGG
    GCAAAGGCGG CCTTCCAGGC CTCCTGCGAG ACATTCTGTG TTGGGGAGGA GGCCAAGGCT
    GCCGCCCAGG ACATCTTCAG CCCCAAAAGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    GCCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG
    TTTCAGGCCA CAGTCCTCTC GGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACC
    ATCCTCGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC GGGGGACCAC
    ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTGGTGGAGG CGCTGCTGAG CCGCGTGGAG
    GACCCGCCAC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAAGG CAGCCCAGGC
    GGCCCTCCTC CCAGCTGGGT GCGGGACCCA CGGCTGCCCC CGTGCACACT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACCTCCCCG CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC
    ACCCTGGCCG AAGAACCCAG CGAGCAGCAG GAGCTCGAGA CCCTCAGTCA GGACCCCCGG
    CGCTATGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC
    CCGTCCGTGG CGCTGCCTGC CCCGCTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG
    TACTATTCCG TCAGCTCGGC CCCCAATGCC CACCCCGGAG AGGTCCACCT CACAGTGGCC
    GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACA
    TGGCTGAGCC AGCTCAAGAC TGGAGACCCC GTGCCCTGCT TCATCAGGGG GGCTCCCTCC
    TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCTGG CACTGGCATC
    GCCCCCTTCC GGGGATTTTG GCAGGAGAGG CTACATGACA TTGAGAGCAA AGGGCTGCAG
    CCCGCCCCCA TGACCCTGGT GTTCGGCTGC CGGTGCTCCC AACTCGACCA TCTCTACCGC
    GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC
    CGGGAACCTG ACAGCCCCAA GACTTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGACGTCACT
    ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGCATCTTGG CGACAGAGGG CGACATGGAG
    CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGTGC GGAAGGGCGG GGGCGCGGCC
    GGCGGGGACA GCCCCGGACG GGGGCGGGGC CTACCGCCGC TCTACACTTC CACCGGGGTT
    TTGATCCTCC GCGCTCTTTC CCTCCCAGGA TCAGCAACGC TATCACGAGG ACATTTTCGG
    CCTCACGCTG CGCACCCAGG AGGTGACAAG CCGTATACGT ACCCAGAGCT TTTCCCTGCA
    GGAACGGCAT CTGCGGGGCG CGGTGCCCTG GGCCTTCGAC CCGCCCGGCC CAGACACCCC
    CGGCCCCTGA GACCCCTCTT GCTTCCCACT GCAGTTCCCG GAGAGAGGGG CTGTCATTCC
    ACTATGGCTC TTCCGCTGTC CTGTCGGCCT TTACCAGGAC CGGCCACCTC TCCCTCTCCT
    CCCAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu02476
    Clone ID Related Accession (Same CDS sequence) XM_006043056.1 , XM_006043056.2
    Accession Version XM_006043056.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product nitric oxide synthase, endothelial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783115.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGCGGCCT GGGGCTGGGG 
    CTGGGCCTCG GGCTATGCGG CAAGCAGGGC CCAGCCTCCC CGGCACCTGA GCCCAGCCGG
    GCCCCCGCAC CCGCCACCCC GCACGCACCA GACCACAGCC CAGCTCCCAA CAGCCCCACG
    CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGAGTGA AGAACTGGGA GCTGGGGAGC
    ATCACCTACG ACACTCTGTG CGCGCAGTCC CAACAGGACG GGCCCTGCAC TCCCAGACGC
    TGCCTGGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACCC GGCCCTCCCC GGGACCTCCA
    CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC
    AAGAGGAGCG GCTCCCAGGC TCACGAAGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA
    TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGCAACGCAC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTCGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGCG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CACAGCGCGC CCCGGGCCGT
    GGAGACTTCC GGATCTGGAA CAGCCAGCTG GTGCGCTACG CGGGCTACAG ACAGCAGGAT
    GGCTCTGTGC GTGGGGACCC AGCCAACGTG GAGATCACAG AGCTCTGCAT CCAGCACGGC
    TGGACCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGACGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTTG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCAGC CCTGGGCCTG CGCTGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC TCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACACG GAACTTGTGT GACCCTCACC GCTACAATAT CCTGGAGGAT
    GTGGCCGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACAGT GTCCTTCATG AAGCACCTGG ACAACGAGCA GAAGGCCAGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCGGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAACTACATC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAGG GGAGCGCAAC CAAGGGTGCC GGCATCACCA GAAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGTTATGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTCGATCCC CGGGTCCTGT GCATGGATGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGC GGCCAGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG CGTCTCCTGC
    TCAGACCCAC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGATAGC
    GCAGGGGCCC TGGGGACCCT CAGGTTCTGT GTGTTCGGAC TGGGCTCCCG GGCGTACCCC
    CACTTCTGCG CCTTCGCGCG AGCGGTGGAC ACCCGGCTGG AAGAGCTGGG AGGGGAGCGG
    CTGCTGCAGC TGGGCCAGGG CGACGAGCTC TGCGGCCAGG AGGAGGCCTT CCGTGGCTGG
    GCAAAGGCGG CCTTCCAGGC CTCCTGCGAG ACATTCTGTG TTGGGGAGGA GGCCAAGGCT
    GCCGCCCAGG ACATCTTCAG CCCCAAAAGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    GCCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG
    TTTCAGGCCA CAGTCCTCTC GGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACC
    ATCCTCGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC GGGGGACCAC
    ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTGGTGGAGG CGCTGCTGAG CCGCGTGGAG
    GACCCGCCAC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAAGG CAGCCCAGGC
    GGCCCTCCTC CCAGCTGGGT GCGGGACCCA CGGCTGCCCC CGTGCACACT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACCTCCCCG CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC
    ACCCTGGCCG AAGAACCCAG CGAGCAGCAG GAGCTCGAGA CCCTCAGTCA GGACCCCCGG
    CGCTATGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC
    CCGTCCGTGG CGCTGCCTGC CCCGCTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG
    TACTATTCCG TCAGCTCGGC CCCCAATGCC CACCCCGGAG AGGTCCACCT CACAGTGGCC
    GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACA
    TGGCTGAGCC AGCTCAAGAC TGGAGACCCC GTGCCCTGCT TCATCAGGGG GGCTCCCTCC
    TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCTGG CACTGGCATC
    GCCCCCTTCC GGGGATTTTG GCAGGAGAGG CTACATGACA TTGAGAGCAA AGGGCTGCAG
    CCCGCCCCCA TGACCCTGGT GTTCGGCTGC CGGTGCTCCC AACTCGACCA TCTCTACCGC
    GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC
    CGGGAACCTG ACAGCCCCAA GACTTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGACGTCACT
    ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGCATCTTGG CGACAGAGGG CGACATGGAG
    CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC
    ATTTTCGGCC TCACGCTGCG CACCCAGGAG GTGACAAGCC GTATACGTAC CCAGAGCTTT
    TCCCTGCAGG AACGGCATCT GCGGGGCGCG GTGCCCTGGG CCTTCGACCC GCCCGGCCCA
    GACACCCCCG GCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006043118.1
    CDS36..3653
    Translation

    Target ORF information:

    RefSeq Version XM_006043056.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006043056.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGCGGCCT GGGGCTGGGG 
    CTGGGCCTCG GGCTATGCGG CAAGCAGGGC CCAGCCTCCC CGGCACCTGA GCCCAGCCGG
    GCCCCCGCAC CCGCCACCCC GCACGCACCA GACCACAGCC CAGCTCCCAA CAGCCCCACG
    CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGAGTGA AGAACTGGGA GCTGGGGAGC
    ATCACCTACG ACACTCTGTG CGCGCAGTCC CAACAGGACG GGCCCTGCAC TCCCAGACGC
    TGCCTGGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACCC GGCCCTCCCC GGGACCTCCA
    CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC
    AAGAGGAGCG GCTCCCAGGC TCACGAAGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA
    TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGCAACGCAC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTCGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGCG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CACAGCGCGC CCCGGGCCGT
    GGAGACTTCC GGATCTGGAA CAGCCAGCTG GTGCGCTACG CGGGCTACAG ACAGCAGGAT
    GGCTCTGTGC GTGGGGACCC AGCCAACGTG GAGATCACAG AGCTCTGCAT CCAGCACGGC
    TGGACCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGACGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTTG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCAGC CCTGGGCCTG CGCTGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC TCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACACG GAACTTGTGT GACCCTCACC GCTACAATAT CCTGGAGGAT
    GTGGCCGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACAGT GTCCTTCATG AAGCACCTGG ACAACGAGCA GAAGGCCAGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCGGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAACTACATC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAGG GGAGCGCAAC CAAGGGTGCC GGCATCACCA GAAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGTTATGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTCGATCCC CGGGTCCTGT GCATGGATGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGC GGCCAGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG CGTCTCCTGC
    TCAGACCCAC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGATAGC
    GCAGGGGCCC TGGGGACCCT CAGGTTCTGT GTGTTCGGAC TGGGCTCCCG GGCGTACCCC
    CACTTCTGCG CCTTCGCGCG AGCGGTGGAC ACCCGGCTGG AAGAGCTGGG AGGGGAGCGG
    CTGCTGCAGC TGGGCCAGGG CGACGAGCTC TGCGGCCAGG AGGAGGCCTT CCGTGGCTGG
    GCAAAGGCGG CCTTCCAGGC CTCCTGCGAG ACATTCTGTG TTGGGGAGGA GGCCAAGGCT
    GCCGCCCAGG ACATCTTCAG CCCCAAAAGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    GCCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG
    TTTCAGGCCA CAGTCCTCTC GGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACC
    ATCCTCGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC GGGGGACCAC
    ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTGGTGGAGG CGCTGCTGAG CCGCGTGGAG
    GACCCGCCAC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAAGG CAGCCCAGGC
    GGCCCTCCTC CCAGCTGGGT GCGGGACCCA CGGCTGCCCC CGTGCACACT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACCTCCCCG CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC
    ACCCTGGCCG AAGAACCCAG CGAGCAGCAG GAGCTCGAGA CCCTCAGTCA GGACCCCCGG
    CGCTATGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC
    CCGTCCGTGG CGCTGCCTGC CCCGCTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG
    TACTATTCCG TCAGCTCGGC CCCCAATGCC CACCCCGGAG AGGTCCACCT CACAGTGGCC
    GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACA
    TGGCTGAGCC AGCTCAAGAC TGGAGACCCC GTGCCCTGCT TCATCAGGGG GGCTCCCTCC
    TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCTGG CACTGGCATC
    GCCCCCTTCC GGGGATTTTG GCAGGAGAGG CTACATGACA TTGAGAGCAA AGGGCTGCAG
    CCCGCCCCCA TGACCCTGGT GTTCGGCTGC CGGTGCTCCC AACTCGACCA TCTCTACCGC
    GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC
    CGGGAACCTG ACAGCCCCAA GACTTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGACGTCACT
    ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGCATCTTGG CGACAGAGGG CGACATGGAG
    CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC
    ATTTTCGGCC TCACGCTGCG CACCCAGGAG GTGACAAGCC GTATACGTAC CCAGAGCTTT
    TCCCTGCAGG AACGGCATCT GCGGGGCGCG GTGCCCTGGG CCTTCGACCC GCCCGGCCCA
    GACACCCCCG GCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu02476
    Clone ID Related Accession (Same CDS sequence) XM_006043056.1 , XM_006043056.2
    Accession Version XM_006043056.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product nitric oxide synthase, endothelial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037552.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006043056.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGCGGCCT GGGGCTGGGG 
    CTGGGCCTCG GGCTATGCGG CAAGCAGGGC CCAGCCTCCC CGGCACCTGA GCCCAGCCGG
    GCCCCCGCAC CCGCCACCCC GCACGCACCA GACCACAGCC CAGCTCCCAA CAGCCCCACG
    CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGAGTGA AGAACTGGGA GCTGGGGAGC
    ATCACCTACG ACACTCTGTG CGCGCAGTCC CAACAGGACG GGCCCTGCAC TCCCAGACGC
    TGCCTGGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACCC GGCCCTCCCC GGGACCTCCA
    CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC
    AAGAGGAGCG GCTCCCAGGC TCACGAAGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA
    TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGCAACGCAC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTCGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGCG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CACAGCGCGC CCCGGGCCGT
    GGAGACTTCC GGATCTGGAA CAGCCAGCTG GTGCGCTACG CGGGCTACAG ACAGCAGGAT
    GGCTCTGTGC GTGGGGACCC AGCCAACGTG GAGATCACAG AGCTCTGCAT CCAGCACGGC
    TGGACCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGACGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTTG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCAGC CCTGGGCCTG CGCTGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC TCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACACG GAACTTGTGT GACCCTCACC GCTACAATAT CCTGGAGGAT
    GTGGCCGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACAGT GTCCTTCATG AAGCACCTGG ACAACGAGCA GAAGGCCAGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCGGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAACTACATC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAGG GGAGCGCAAC CAAGGGTGCC GGCATCACCA GAAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGTTATGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTCGATCCC CGGGTCCTGT GCATGGATGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGC GGCCAGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG CGTCTCCTGC
    TCAGACCCAC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGATAGC
    GCAGGGGCCC TGGGGACCCT CAGGTTCTGT GTGTTCGGAC TGGGCTCCCG GGCGTACCCC
    CACTTCTGCG CCTTCGCGCG AGCGGTGGAC ACCCGGCTGG AAGAGCTGGG AGGGGAGCGG
    CTGCTGCAGC TGGGCCAGGG CGACGAGCTC TGCGGCCAGG AGGAGGCCTT CCGTGGCTGG
    GCAAAGGCGG CCTTCCAGGC CTCCTGCGAG ACATTCTGTG TTGGGGAGGA GGCCAAGGCT
    GCCGCCCAGG ACATCTTCAG CCCCAAAAGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    GCCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG
    TTTCAGGCCA CAGTCCTCTC GGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACC
    ATCCTCGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC GGGGGACCAC
    ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTGGTGGAGG CGCTGCTGAG CCGCGTGGAG
    GACCCGCCAC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAAGG CAGCCCAGGC
    GGCCCTCCTC CCAGCTGGGT GCGGGACCCA CGGCTGCCCC CGTGCACACT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACCTCCCCG CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC
    ACCCTGGCCG AAGAACCCAG CGAGCAGCAG GAGCTCGAGA CCCTCAGTCA GGACCCCCGG
    CGCTATGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC
    CCGTCCGTGG CGCTGCCTGC CCCGCTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG
    TACTATTCCG TCAGCTCGGC CCCCAATGCC CACCCCGGAG AGGTCCACCT CACAGTGGCC
    GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACA
    TGGCTGAGCC AGCTCAAGAC TGGAGACCCC GTGCCCTGCT TCATCAGGGG GGCTCCCTCC
    TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCTGG CACTGGCATC
    GCCCCCTTCC GGGGATTTTG GCAGGAGAGG CTACATGACA TTGAGAGCAA AGGGCTGCAG
    CCCGCCCCCA TGACCCTGGT GTTCGGCTGC CGGTGCTCCC AACTCGACCA TCTCTACCGC
    GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC
    CGGGAACCTG ACAGCCCCAA GACTTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGACGTCACT
    ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGCATCTTGG CGACAGAGGG CGACATGGAG
    CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC
    ATTTTCGGCC TCACGCTGCG CACCCAGGAG GTGACAAGCC GTATACGTAC CCAGAGCTTT
    TCCCTGCAGG AACGGCATCT GCGGGGCGCG GTGCCCTGGG CCTTCGACCC GCCCGGCCCA
    GACACCCCCG GCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006043118.1
    CDS55..3672
    Translation

    Target ORF information:

    RefSeq Version XM_006043056.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis nitric oxide synthase 3 (NOS3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006043056.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGCGGCCT GGGGCTGGGG 
    CTGGGCCTCG GGCTATGCGG CAAGCAGGGC CCAGCCTCCC CGGCACCTGA GCCCAGCCGG
    GCCCCCGCAC CCGCCACCCC GCACGCACCA GACCACAGCC CAGCTCCCAA CAGCCCCACG
    CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGAGTGA AGAACTGGGA GCTGGGGAGC
    ATCACCTACG ACACTCTGTG CGCGCAGTCC CAACAGGACG GGCCCTGCAC TCCCAGACGC
    TGCCTGGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACCC GGCCCTCCCC GGGACCTCCA
    CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC
    AAGAGGAGCG GCTCCCAGGC TCACGAAGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA
    TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGCAACGCAC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTCGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGCG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CACAGCGCGC CCCGGGCCGT
    GGAGACTTCC GGATCTGGAA CAGCCAGCTG GTGCGCTACG CGGGCTACAG ACAGCAGGAT
    GGCTCTGTGC GTGGGGACCC AGCCAACGTG GAGATCACAG AGCTCTGCAT CCAGCACGGC
    TGGACCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGACGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTTG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCAGC CCTGGGCCTG CGCTGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC TCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACACG GAACTTGTGT GACCCTCACC GCTACAATAT CCTGGAGGAT
    GTGGCCGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACAGT GTCCTTCATG AAGCACCTGG ACAACGAGCA GAAGGCCAGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCGGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAACTACATC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAGG GGAGCGCAAC CAAGGGTGCC GGCATCACCA GAAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGTTATGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTCGATCCC CGGGTCCTGT GCATGGATGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGC GGCCAGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG CGTCTCCTGC
    TCAGACCCAC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGATAGC
    GCAGGGGCCC TGGGGACCCT CAGGTTCTGT GTGTTCGGAC TGGGCTCCCG GGCGTACCCC
    CACTTCTGCG CCTTCGCGCG AGCGGTGGAC ACCCGGCTGG AAGAGCTGGG AGGGGAGCGG
    CTGCTGCAGC TGGGCCAGGG CGACGAGCTC TGCGGCCAGG AGGAGGCCTT CCGTGGCTGG
    GCAAAGGCGG CCTTCCAGGC CTCCTGCGAG ACATTCTGTG TTGGGGAGGA GGCCAAGGCT
    GCCGCCCAGG ACATCTTCAG CCCCAAAAGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    GCCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG
    TTTCAGGCCA CAGTCCTCTC GGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACC
    ATCCTCGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC GGGGGACCAC
    ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTGGTGGAGG CGCTGCTGAG CCGCGTGGAG
    GACCCGCCAC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAAGG CAGCCCAGGC
    GGCCCTCCTC CCAGCTGGGT GCGGGACCCA CGGCTGCCCC CGTGCACACT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACCTCCCCG CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC
    ACCCTGGCCG AAGAACCCAG CGAGCAGCAG GAGCTCGAGA CCCTCAGTCA GGACCCCCGG
    CGCTATGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC
    CCGTCCGTGG CGCTGCCTGC CCCGCTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG
    TACTATTCCG TCAGCTCGGC CCCCAATGCC CACCCCGGAG AGGTCCACCT CACAGTGGCC
    GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACA
    TGGCTGAGCC AGCTCAAGAC TGGAGACCCC GTGCCCTGCT TCATCAGGGG GGCTCCCTCC
    TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCTGG CACTGGCATC
    GCCCCCTTCC GGGGATTTTG GCAGGAGAGG CTACATGACA TTGAGAGCAA AGGGCTGCAG
    CCCGCCCCCA TGACCCTGGT GTTCGGCTGC CGGTGCTCCC AACTCGACCA TCTCTACCGC
    GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC
    CGGGAACCTG ACAGCCCCAA GACTTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGACGTCACT
    ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGCATCTTGG CGACAGAGGG CGACATGGAG
    CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC
    ATTTTCGGCC TCACGCTGCG CACCCAGGAG GTGACAAGCC GTATACGTAC CCAGAGCTTT
    TCCCTGCAGG AACGGCATCT GCGGGGCGCG GTGCCCTGGG CCTTCGACCC GCCCGGCCCA
    GACACCCCCG GCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.