NCKAP1 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following NCKAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCKAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu26145 XM_006071782.1
Latest version!
Bubalus bubalis NCK-associated protein 1 (NCKAP1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBu55345 XM_006071782.2
Latest version!
Bubalus bubalis NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBu55346 XM_025273747.1
Latest version!
Bubalus bubalis NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBu55347 XM_025273748.1
Latest version!
Bubalus bubalis NCK associated protein 1 (NCKAP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu26145
    Clone ID Related Accession (Same CDS sequence) XM_006071782.1
    Accession Version XM_006071782.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product LOW QUALITY PROTEIN: nck-associated protein 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785461.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CCGTGCTGCA GCCTAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCTT GTGGAGACCC CAAGGCAAAA CCATCCTACC TTATTGACAA AAACCTGGAA
    TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACTCGCAA CAACAATCAA
    CAGCTTGCAC AACTGCAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC
    TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT
    GATGTTTGCC AAGTCTTCTT TGACATTACT GTGAACTTTG ATTTAACAAA GAACTACTTA
    GATTTGATCA TAACATATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG
    GCAATCATTG GATTATACAA CTATGCTCAT GAGATGACCC ATGGGGCAAG TGACCGAGAA
    TACCCACGCC TTGGTCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA AATGATGGAA
    GAATTTGTGC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA GATGGTGTAT
    CCTCGAAGGA ACCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTGCTGAG TCTCATCAGT
    GCCCCCAGCA CAATGCTTAA TCCCGCACAG TCCGACACTA TGCCTTGTGA ATATCTCTCT
    TTGGATGCCA TGGAAAAATG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT
    ACTGATGCAA CAGCGCTGAA CCTTTGGAAG CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT
    CTTTTTCGGG ACGAAGTTTT CCACATTCAT AAAGCTGCAG AAGACTTATT TGTAAACATT
    CGAGGCTATA ATAAGCGTAT TAATGACATA AGAGAATGCA AAGAGGCAGC TGTGTCACAT
    GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGGT CTGCACTGAA AGAATTGGCT
    ACTGTCCTCT CTGATCAGCC TGGCTTGCTA GGTCCTAAGG CACTTTTTGT TTTTATGGCA
    TTATCCTTTG CCCGTGATGA AATTATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG
    AAGAGTGCAG ATGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA TATGGAAGAG
    CTTAGAGCAC ATGTAAGGAA ATACGGACCT GTAATGCAGA GATATTACGT GCAGTACCTT
    TCTGGCTTTG ATGCTGTTGT CCTCAATGAG CTTGTGCAGA ATCTTTCTGT TTGCCCTGAA
    GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCTCTAAG TGTAAAACAA
    GTTGAAGATG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TCGATTACAG
    GCATATACTA GTGTCTCTAA GGCTTCACTT AGCCTTTCAG ATCACAGAGA ACTTGGAAAG
    ATGATGAATA CAATAATTTT TCACACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG
    GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA
    CAGTGTTTGG AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT
    CATTTTATGA GTTGCACACA TGAACTGTGT CCAGAAGAGA GACATCATAT TGGAGATCGC
    AGTCTTTCCT TATGTAATAT GTTCCTTGAT GAAATGGCCA AACAAGCTCG AAATCTCATC
    ACTGATATTT GCACAGAACA GTGTACTCTT AGTGACCAGT TGTTGCCCAA GCATTGTGCC
    AAAACCATCA GTCAAGCAGT GAATAAGAAG TCAAAAAAAC AGACTGGTAA GAAAGGGGAA
    CCTGAAAGGG AAAAACCAGG AGTTGAAAGC ATGAGGAAAA ACAGGCTGGT CGTGACCAAC
    CTTGATAAGT TGCACACTGC ACTTTCCGAG TTGTGTTTCT CTATAAATTA TGTACCAAAC
    ATGGTGGTTT GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATC
    CGCTTTACTA AGTCAATTGT TGGAATGACT ATGTATAATC AAGCCACACA GGAAATTGCA
    AAACCATCAG AGCTTCTAAC AAGTGTAAGA GCATACATGA CTGTTCTCCA GTCAATAGAA
    AACTATGTGC AAATTGATAT TACAAGAGTA TTTAATAACG TTCTTCTTCA ACAAACACAA
    CATTTAGACA GTCATGGAGA ACCAACCATT ACAAGTCTGT ACACAAATTG GTATTTGGAA
    ACTTTGTTAA GACAAGTCAG CAATGGTCAT ATAGCTTATT TCCCTGCAAT GAAAGCATTT
    GTGAACTTAC CCACGGAAAA TGAATTAACG TTTAATGCAG AGGAATATTC TGACATATCA
    GAAATGAGGT CATTATCAGA GCTGCTTGGC CCATATGGTA TGAAGTTCCT AAGTGAAAGT
    CTTATGTGGC ATATTTCATC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT
    GATGTGCTAA CACAAATGCG GACCAGCTTT GACAAACCCG ACCAGATGGC TGCCCTCTTT
    AAAAGATTAT CGTCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA
    TCCTTCCGAT CACTGGCACA GGAAGCACTA AGAGATGTCT TGTCCTACCA CATTCCTTTT
    CTTGTCAGTT CAATTGAAGA TTTCAAGGAT CACATTCCAA GGGAAACGGA TATGAAGGTT
    GCAATGAATG TGTATGAATT ATCATCAGCT GCTGGATTAC CTTGTGAGAT TGATCCTGCC
    TTGGTGGTAG CTCTTTCCTC ACAAAAATCA GAAAACATAA GTCCAGAAGA AGAATATAAA
    ATTGCCTGCC TCCTCATGGT GTTTGTGGCC GTTTCTTTGC CCACACTGGC CAGTAATGTG
    ATGTCTCAGT ATAGCCCTGC TATAGAAGGG CACTGCAACA ACATACATTG CTTGGCCAAA
    GCCATCAACC AGATCGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT CGAAGATCGG
    CTTAAAGAAT TTTTGGCGCT TGCATCCTCC AGTCTGCTGA AAATTGGCCA GGAGACAGAT
    AAAACAACTA CACGAAATAG AGAATCTGTT TATTTACTGC TAGATATGAT TGTGCAGGAA
    TCCCCATTCC TGACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT
    GCATACCATG CTGTCTACAA GCAAAGTGTT ACATCCTCTG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006071844.1
    CDS1..3405
    Translation

    Target ORF information:

    RefSeq Version XM_006071782.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NCK-associated protein 1 (NCKAP1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006071782.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CCGTGCTGCA GCCTAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCTT GTGGAGACCC CAAGGCAAAA CCATCCTACC TTATTGACAA AAACCTGGAA
    TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACTCGCAA CAACAATCAA
    CAGCTTGCAC AACTGCAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC
    TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT
    GATGTTTGCC AAGTCTTCTT TGACATTACT GTGAACTTTG ATTTAACAAA GAACTACTTA
    GATTTGATCA TAACATATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG
    GCAATCATTG GATTATACAA CTATGCTCAT GAGATGACCC ATGGGGCAAG TGACCGAGAA
    TACCCACGCC TTGGTCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA AATGATGGAA
    GAATTTGTGC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA GATGGTGTAT
    CCTCGAAGGA ACCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTGCTGAG TCTCATCAGT
    GCCCCCAGCA CAATGCTTAA TCCCGCACAG TCCGACACTA TGCCTTGTGA ATATCTCTCT
    TTGGATGCCA TGGAAAAATG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT
    ACTGATGCAA CAGCGCTGAA CCTTTGGAAG CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT
    CTTTTTCGGG ACGAAGTTTT CCACATTCAT AAAGCTGCAG AAGACTTATT TGTAAACATT
    CGAGGCTATA ATAAGCGTAT TAATGACATA AGAGAATGCA AAGAGGCAGC TGTGTCACAT
    GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGGT CTGCACTGAA AGAATTGGCT
    ACTGTCCTCT CTGATCAGCC TGGCTTGCTA GGTCCTAAGG CACTTTTTGT TTTTATGGCA
    TTATCCTTTG CCCGTGATGA AATTATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG
    AAGAGTGCAG ATGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA TATGGAAGAG
    CTTAGAGCAC ATGTAAGGAA ATACGGACCT GTAATGCAGA GATATTACGT GCAGTACCTT
    TCTGGCTTTG ATGCTGTTGT CCTCAATGAG CTTGTGCAGA ATCTTTCTGT TTGCCCTGAA
    GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCTCTAAG TGTAAAACAA
    GTTGAAGATG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TCGATTACAG
    GCATATACTA GTGTCTCTAA GGCTTCACTT AGCCTTTCAG ATCACAGAGA ACTTGGAAAG
    ATGATGAATA CAATAATTTT TCACACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG
    GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA
    CAGTGTTTGG AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT
    CATTTTATGA GTTGCACACA TGAACTGTGT CCAGAAGAGA GACATCATAT TGGAGATCGC
    AGTCTTTCCT TATGTAATAT GTTCCTTGAT GAAATGGCCA AACAAGCTCG AAATCTCATC
    ACTGATATTT GCACAGAACA GTGTACTCTT AGTGACCAGT TGTTGCCCAA GCATTGTGCC
    AAAACCATCA GTCAAGCAGT GAATAAGAAG TCAAAAAAAC AGACTGGTAA GAAAGGGGAA
    CCTGAAAGGG AAAAACCAGG AGTTGAAAGC ATGAGGAAAA ACAGGCTGGT CGTGACCAAC
    CTTGATAAGT TGCACACTGC ACTTTCCGAG TTGTGTTTCT CTATAAATTA TGTACCAAAC
    ATGGTGGTTT GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATC
    CGCTTTACTA AGTCAATTGT TGGAATGACT ATGTATAATC AAGCCACACA GGAAATTGCA
    AAACCATCAG AGCTTCTAAC AAGTGTAAGA GCATACATGA CTGTTCTCCA GTCAATAGAA
    AACTATGTGC AAATTGATAT TACAAGAGTA TTTAATAACG TTCTTCTTCA ACAAACACAA
    CATTTAGACA GTCATGGAGA ACCAACCATT ACAAGTCTGT ACACAAATTG GTATTTGGAA
    ACTTTGTTAA GACAAGTCAG CAATGGTCAT ATAGCTTATT TCCCTGCAAT GAAAGCATTT
    GTGAACTTAC CCACGGAAAA TGAATTAACG TTTAATGCAG AGGAATATTC TGACATATCA
    GAAATGAGGT CATTATCAGA GCTGCTTGGC CCATATGGTA TGAAGTTCCT AAGTGAAAGT
    CTTATGTGGC ATATTTCATC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT
    GATGTGCTAA CACAAATGCG GACCAGCTTT GACAAACCCG ACCAGATGGC TGCCCTCTTT
    AAAAGATTAT CGTCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA
    TCCTTCCGAT CACTGGCACA GGAAGCACTA AGAGATGTCT TGTCCTACCA CATTCCTTTT
    CTTGTCAGTT CAATTGAAGA TTTCAAGGAT CACATTCCAA GGGAAACGGA TATGAAGGTT
    GCAATGAATG TGTATGAATT ATCATCAGCT GCTGGATTAC CTTGTGAGAT TGATCCTGCC
    TTGGTGGTAG CTCTTTCCTC ACAAAAATCA GAAAACATAA GTCCAGAAGA AGAATATAAA
    ATTGCCTGCC TCCTCATGGT GTTTGTGGCC GTTTCTTTGC CCACACTGGC CAGTAATGTG
    ATGTCTCAGT ATAGCCCTGC TATAGAAGGG CACTGCAACA ACATACATTG CTTGGCCAAA
    GCCATCAACC AGATCGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT CGAAGATCGG
    CTTAAAGAAT TTTTGGCGCT TGCATCCTCC AGTCTGCTGA AAATTGGCCA GGAGACAGAT
    AAAACAACTA CACGAAATAG AGAATCTGTT TATTTACTGC TAGATATGAT TGTGCAGGAA
    TCCCCATTCC TGACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT
    GCATACCATG CTGTCTACAA GCAAAGTGTT ACATCCTCTG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu55345
    Clone ID Related Accession (Same CDS sequence) XM_006071782.2
    Accession Version XM_006071782.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product nck-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006071782.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CCGTGCTGCA GCCTAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCTT GTGGAGACCC CAAGGCAAAA CCATCCTACC TTATTGACAA AAACCTGGAA
    TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACTCGCAA CAACAATCAA
    CAGCTTGCAC AACTGCAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC
    TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT
    GATGTTTGCC AAGTCTTCTT TGACATTACT GTGAACTTTG ATTTAACAAA GAACTACTTA
    GATTTGATCA TAACATATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG
    GCAATCATTG GATTATACAA CTATGCTCAT GAGATGACCC ATGGGGCAAG TGACCGAGAA
    TACCCACGCC TTGGTCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA AATGATGGAA
    GAATTTGTGC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA GATGGTGTAT
    CCTCGAAGGA ACCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTGCTGAG TCTCATCAGT
    GCCCCCAGCA CAATGCTTAA TCCCGCACAG TCCGACACTA TGCCTTGTGA ATATCTCTCT
    TTGGATGCCA TGGAAAAATG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT
    ACTGATGCAA CAGCGCTGAA CCTTTGGAAG CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT
    CTTTTTCGGG ACGAAGTTTT CCACATTCAT AAAGCTGCAG AAGACTTATT TGTAAACATT
    CGAGGCTATA ATAAGCGTAT TAATGACATA AGAGAATGCA AAGAGGCAGC TGTGTCACAT
    GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGGT CTGCACTGAA AGAATTGGCT
    ACTGTCCTCT CTGATCAGCC TGGCTTGCTA GGTCCTAAGG CACTTTTTGT TTTTATGGCA
    TTATCCTTTG CCCGTGATGA AATTATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG
    AAGAGTGCAG ATGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA TATGGAAGAG
    CTTAGAGCAC ATGTAAGGAA ATACGGACCT GTAATGCAGA GATATTACGT GCAGTACCTT
    TCTGGCTTTG ATGCTGTTGT CCTCAATGAG CTTGTGCAGA ATCTTTCTGT TTGCCCTGAA
    GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCTCTAAG TGTAAAACAA
    GTTGAAGATG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TCGATTACAG
    GCATATACTA GTGTCTCTAA GGCTTCACTT AGCCTTTCAG ATCACAGAGA ACTTGGAAAG
    ATGATGAATA CAATAATTTT TCACACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG
    GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA
    CAGTGTTTGG AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT
    CATTTTATGA GTTGCACACA TGAACTATGT CCAGAAGAGA GACATCATAT TGGAGATCGC
    AGTCTTTCCT TATGTAATAT GTTCCTTGAT GAAATGGCCA AACAAGCTCG AAATCTCATC
    ACTGATATTT GCACAGAACA GTGTACTCTT AGTGACCAGT TGTTGCCCAA GCATTGTGCC
    AAAACCATCA GTCAAGCAGT GAATAAGAAG TCAAAAAAAC AGACTGGTAA GAAAGGGGAA
    CCTGAAAGGG AAAAACCAGG AGTTGAAAGC ATGAGGAAAA ACAGGCTGGT CGTGACCAAC
    CTTGATAAGT TGCACACTGC ACTTTCCGAG TTGTGTTTCT CTATAAATTA TGTACCAAAC
    ATGGTGGTTT GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATC
    CGCTTTACTA AGTCAATTGT TGGAATGACT ATGTATAATC AAGCCACACA GGAAATTGCA
    AAACCATCAG AGCTTCTAAC AAGTGTAAGA GCATACATGA CTGTTCTCCA GTCAATAGAA
    AACTATGTGC AAATTGATAT TACAAGAGTA TTTAATAATG TTCTTCTTCA ACAAACACAA
    CATTTAGACA GTCATGGAGA ACCAACCATT ACAAGTCTGT ACACAAATTG GTATTTGGAA
    ACTTTGTTAA GACAAGTCAG CAATGGTCAT ATAGCTTATT TCCCTGCAAT GAAAGCATTT
    GTGAACTTAC CCACGGAAAA TGAATTAACG TTTAATGCAG AGGAATATTC TGACATATCA
    GAAATGAGGT CATTATCAGA GCTGCTTGGC CCATATGGTA TGAAGTTCCT AAGTGAAAGT
    CTTATGTGGC ATATTTCATC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT
    GATGTGCTAA CACAAATGCG GACCAGCTTT GACAAACCCG ACCAGATGGC TGCCCTCTTT
    AAAAGATTAT CGTCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA
    TCTTTCCGAT CACTGGCACA GGAAGCACTA AGAGATGTCT TGTCCTACCA CATTCCTTTT
    CTTGTCAGTT CAATTGAAGA TTTCAAGGAT CACATTCCAA GGGAAACGGA TATGAAGGTT
    GCAATGAATG TGTATGAATT ATCATCAGCT GCTGGATTAC CTTGTGAGAT TGATCCTGCC
    TTGGTGGTAG CTCTTTCCTC ACAAAAATCA GAAAACATAA GTCCAGAAGA AGAATATAAA
    ATTGCCTGCC TCCTCATGGT GTTTGTGGCC GTTTCTTTGC CCACACTGGC CAGTAATGTG
    ATGTCTCAGT ATAGCCCTGC TATAGAAGGG CACTGCAACA ACATACATTG CTTGGCCAAA
    GCCATCAACC AGATCGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT CGAAGATCGG
    CTTAAAGAAT TTTTGGCGCT TGCATCCTCC AGTCTGCTGA AAATTGGCCA GGAGACAGAT
    AAAACAACTA CACGAAATAG AGAATCTGTT TATTTACTGC TAGATATGAT TGTGCAGGAA
    TCCCCATTCC TGACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT
    GCATACCATG CTGTCTACAA GCAAAGTGTT ACATCCTCTG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006071844.1
    CDS336..3740
    Translation

    Target ORF information:

    RefSeq Version XM_006071782.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006071782.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CCGTGCTGCA GCCTAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCTT GTGGAGACCC CAAGGCAAAA CCATCCTACC TTATTGACAA AAACCTGGAA
    TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACTCGCAA CAACAATCAA
    CAGCTTGCAC AACTGCAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC
    TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT
    GATGTTTGCC AAGTCTTCTT TGACATTACT GTGAACTTTG ATTTAACAAA GAACTACTTA
    GATTTGATCA TAACATATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG
    GCAATCATTG GATTATACAA CTATGCTCAT GAGATGACCC ATGGGGCAAG TGACCGAGAA
    TACCCACGCC TTGGTCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA AATGATGGAA
    GAATTTGTGC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA GATGGTGTAT
    CCTCGAAGGA ACCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTGCTGAG TCTCATCAGT
    GCCCCCAGCA CAATGCTTAA TCCCGCACAG TCCGACACTA TGCCTTGTGA ATATCTCTCT
    TTGGATGCCA TGGAAAAATG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT
    ACTGATGCAA CAGCGCTGAA CCTTTGGAAG CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT
    CTTTTTCGGG ACGAAGTTTT CCACATTCAT AAAGCTGCAG AAGACTTATT TGTAAACATT
    CGAGGCTATA ATAAGCGTAT TAATGACATA AGAGAATGCA AAGAGGCAGC TGTGTCACAT
    GCTGGTTCAA TGCACAGAGA AAGACGCAAG TTTTTAAGGT CTGCACTGAA AGAATTGGCT
    ACTGTCCTCT CTGATCAGCC TGGCTTGCTA GGTCCTAAGG CACTTTTTGT TTTTATGGCA
    TTATCCTTTG CCCGTGATGA AATTATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG
    AAGAGTGCAG ATGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA TATGGAAGAG
    CTTAGAGCAC ATGTAAGGAA ATACGGACCT GTAATGCAGA GATATTACGT GCAGTACCTT
    TCTGGCTTTG ATGCTGTTGT CCTCAATGAG CTTGTGCAGA ATCTTTCTGT TTGCCCTGAA
    GATGAATCAA TCATCATGTC CTCTTTTGTT AACACTATGA CTTCTCTAAG TGTAAAACAA
    GTTGAAGATG GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TCGATTACAG
    GCATATACTA GTGTCTCTAA GGCTTCACTT AGCCTTTCAG ATCACAGAGA ACTTGGAAAG
    ATGATGAATA CAATAATTTT TCACACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG
    GAAACATCAG ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA
    CAGTGTTTGG AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT
    CATTTTATGA GTTGCACACA TGAACTATGT CCAGAAGAGA GACATCATAT TGGAGATCGC
    AGTCTTTCCT TATGTAATAT GTTCCTTGAT GAAATGGCCA AACAAGCTCG AAATCTCATC
    ACTGATATTT GCACAGAACA GTGTACTCTT AGTGACCAGT TGTTGCCCAA GCATTGTGCC
    AAAACCATCA GTCAAGCAGT GAATAAGAAG TCAAAAAAAC AGACTGGTAA GAAAGGGGAA
    CCTGAAAGGG AAAAACCAGG AGTTGAAAGC ATGAGGAAAA ACAGGCTGGT CGTGACCAAC
    CTTGATAAGT TGCACACTGC ACTTTCCGAG TTGTGTTTCT CTATAAATTA TGTACCAAAC
    ATGGTGGTTT GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATC
    CGCTTTACTA AGTCAATTGT TGGAATGACT ATGTATAATC AAGCCACACA GGAAATTGCA
    AAACCATCAG AGCTTCTAAC AAGTGTAAGA GCATACATGA CTGTTCTCCA GTCAATAGAA
    AACTATGTGC AAATTGATAT TACAAGAGTA TTTAATAATG TTCTTCTTCA ACAAACACAA
    CATTTAGACA GTCATGGAGA ACCAACCATT ACAAGTCTGT ACACAAATTG GTATTTGGAA
    ACTTTGTTAA GACAAGTCAG CAATGGTCAT ATAGCTTATT TCCCTGCAAT GAAAGCATTT
    GTGAACTTAC CCACGGAAAA TGAATTAACG TTTAATGCAG AGGAATATTC TGACATATCA
    GAAATGAGGT CATTATCAGA GCTGCTTGGC CCATATGGTA TGAAGTTCCT AAGTGAAAGT
    CTTATGTGGC ATATTTCATC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT
    GATGTGCTAA CACAAATGCG GACCAGCTTT GACAAACCCG ACCAGATGGC TGCCCTCTTT
    AAAAGATTAT CGTCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA
    TCTTTCCGAT CACTGGCACA GGAAGCACTA AGAGATGTCT TGTCCTACCA CATTCCTTTT
    CTTGTCAGTT CAATTGAAGA TTTCAAGGAT CACATTCCAA GGGAAACGGA TATGAAGGTT
    GCAATGAATG TGTATGAATT ATCATCAGCT GCTGGATTAC CTTGTGAGAT TGATCCTGCC
    TTGGTGGTAG CTCTTTCCTC ACAAAAATCA GAAAACATAA GTCCAGAAGA AGAATATAAA
    ATTGCCTGCC TCCTCATGGT GTTTGTGGCC GTTTCTTTGC CCACACTGGC CAGTAATGTG
    ATGTCTCAGT ATAGCCCTGC TATAGAAGGG CACTGCAACA ACATACATTG CTTGGCCAAA
    GCCATCAACC AGATCGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT CGAAGATCGG
    CTTAAAGAAT TTTTGGCGCT TGCATCCTCC AGTCTGCTGA AAATTGGCCA GGAGACAGAT
    AAAACAACTA CACGAAATAG AGAATCTGTT TATTTACTGC TAGATATGAT TGTGCAGGAA
    TCCCCATTCC TGACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT
    GCATACCATG CTGTCTACAA GCAAAGTGTT ACATCCTCTG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu55346
    Clone ID Related Accession (Same CDS sequence) XM_025273747.1
    Accession Version XM_025273747.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product nck-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CCGTGCTGCA GCCTAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC TTGTGGAGAC
    CCCAAGGCAA AACCATCCTA CCTTATTGAC AAAAACCTGG AATCTGCTGT GAAATTCATA
    GTCAGAAAAT TCCCTGCTGT AGAAACTCGC AACAACAATC AACAGCTTGC ACAACTGCAG
    AAAGAAAAAT CAGAGATTCT GAAAAATCTG GCATTATATT ACTTCACATT TGTAGATGTT
    ATGGAATTTA AGGACCATGT TTGTGAATTG CTGAATACTA TTGATGTTTG CCAAGTCTTC
    TTTGACATTA CTGTGAACTT TGATTTAACA AAGAACTACT TAGATTTGAT CATAACATAT
    ACAACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCAATCAT TGGATTATAC
    AACTATGCTC ATGAGATGAC CCATGGGGCA AGTGACCGAG AATACCCACG CCTTGGTCAG
    ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAAATGATGG AAGAATTTGT GCCCCATAGC
    AAGTCTCTTT CAGATGCACT AATTTCTCTT CAGATGGTGT ATCCTCGAAG GAACCTTTCA
    GCTGACCAGT GGAGAAATGC CCAGTTGCTG AGTCTCATCA GTGCCCCCAG CACAATGCTT
    AATCCCGCAC AGTCCGACAC TATGCCTTGT GAATATCTCT CTTTGGATGC CATGGAAAAA
    TGGATTATCT TTGGCTTTAT TTTGTGCCAT GGGATCCTAA ATACTGATGC AACAGCGCTG
    AACCTTTGGA AGCTAGCTCT TCAAAGTAGC TCTTGCCTCT CTCTTTTTCG GGACGAAGTT
    TTCCACATTC ATAAAGCTGC AGAAGACTTA TTTGTAAACA TTCGAGGCTA TAATAAGCGT
    ATTAATGACA TAAGAGAATG CAAAGAGGCA GCTGTGTCAC ATGCTGGTTC AATGCACAGA
    GAAAGACGCA AGTTTTTAAG GTCTGCACTG AAAGAATTGG CTACTGTCCT CTCTGATCAG
    CCTGGCTTGC TAGGTCCTAA GGCACTTTTT GTTTTTATGG CATTATCCTT TGCCCGTGAT
    GAAATTATCT GGCTACTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGATGACTTT
    ATAGATAAGC ACATTGCTGA ATTAATATTT TATATGGAAG AGCTTAGAGC ACATGTAAGG
    AAATACGGAC CTGTAATGCA GAGATATTAC GTGCAGTACC TTTCTGGCTT TGATGCTGTT
    GTCCTCAATG AGCTTGTGCA GAATCTTTCT GTTTGCCCTG AAGATGAATC AATCATCATG
    TCCTCTTTTG TTAACACTAT GACTTCTCTA AGTGTAAAAC AAGTTGAAGA TGGGGAAGTA
    TTTGATTTCA GAGGAATGAG ATTAGATTGG TTTCGATTAC AGGCATATAC TAGTGTCTCT
    AAGGCTTCAC TTAGCCTTTC AGATCACAGA GAACTTGGAA AGATGATGAA TACAATAATT
    TTTCACACAA AAATGGTAGA TTCCTTGGTG GAAATGTTGG TGGAAACATC AGATCTCTCC
    ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AACAGTGTTT GGAGTTACCC
    TCTCAATCAA GATACTCAAT TGCATTTCCA CTACTTTGCA CTCATTTTAT GAGTTGCACA
    CATGAACTAT GTCCAGAAGA GAGACATCAT ATTGGAGATC GCAGTCTTTC CTTATGTAAT
    ATGTTCCTTG ATGAAATGGC CAAACAAGCT CGAAATCTCA TCACTGATAT TTGCACAGAA
    CAGTGTACTC TTAGTGACCA GTTGTTGCCC AAGCATTGTG CCAAAACCAT CAGTCAAGCA
    GTGAATAAGA AGTCAAAAAA ACAGACTGGT AAGAAAGGGG AACCTGAAAG GGAAAAACCA
    GGAGTTGAAA GCATGAGGAA AAACAGGCTG GTCGTGACCA ACCTTGATAA GTTGCACACT
    GCACTTTCCG AGTTGTGTTT CTCTATAAAT TATGTACCAA ACATGGTGGT TTGGGAACAT
    ACCTTTACCC CACGAGAATA TTTGACTTCT CATCTGGAAA TCCGCTTTAC TAAGTCAATT
    GTTGGAATGA CTATGTATAA TCAAGCCACA CAGGAAATTG CAAAACCATC AGAGCTTCTA
    ACAAGTGTAA GAGCATACAT GACTGTTCTC CAGTCAATAG AAAACTATGT GCAAATTGAT
    ATTACAAGAG TATTTAATAA TGTTCTTCTT CAACAAACAC AACATTTAGA CAGTCATGGA
    GAACCAACCA TTACAAGTCT GTACACAAAT TGGTATTTGG AAACTTTGTT AAGACAAGTC
    AGCAATGGTC ATATAGCTTA TTTCCCTGCA ATGAAAGCAT TTGTGAACTT ACCCACGGAA
    AATGAATTAA CGTTTAATGC AGAGGAATAT TCTGACATAT CAGAAATGAG GTCATTATCA
    GAGCTGCTTG GCCCATATGG TATGAAGTTC CTAAGTGAAA GTCTTATGTG GCATATTTCA
    TCACAAGTTG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTGCT AACACAAATG
    CGGACCAGCT TTGACAAACC CGACCAGATG GCTGCCCTCT TTAAAAGATT ATCGTCTGTT
    GACAGTGTCT TGAAGAGGAT GACAATAATT GGTGTAATTT TATCTTTCCG ATCACTGGCA
    CAGGAAGCAC TAAGAGATGT CTTGTCCTAC CACATTCCTT TTCTTGTCAG TTCAATTGAA
    GATTTCAAGG ATCACATTCC AAGGGAAACG GATATGAAGG TTGCAATGAA TGTGTATGAA
    TTATCATCAG CTGCTGGATT ACCTTGTGAG ATTGATCCTG CCTTGGTGGT AGCTCTTTCC
    TCACAAAAAT CAGAAAACAT AAGTCCAGAA GAAGAATATA AAATTGCCTG CCTCCTCATG
    GTGTTTGTGG CCGTTTCTTT GCCCACACTG GCCAGTAATG TGATGTCTCA GTATAGCCCT
    GCTATAGAAG GGCACTGCAA CAACATACAT TGCTTGGCCA AAGCCATCAA CCAGATCGCT
    GCAGCTTTGT TTACAATTCA CAAAGGAAGC ATCGAAGATC GGCTTAAAGA ATTTTTGGCG
    CTTGCATCCT CCAGTCTGCT GAAAATTGGC CAGGAGACAG ATAAAACAAC TACACGAAAT
    AGAGAATCTG TTTATTTACT GCTAGATATG ATTGTGCAGG AATCCCCATT CCTGACAATG
    GATCTTTTGG AATCTTGTTT TCCTTATGTC TTGCTGAGAA ATGCATACCA TGCTGTCTAC
    AAGCAAAGTG TTACATCCTC TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025129532.1
    CDS335..3721
    Translation

    Target ORF information:

    RefSeq Version XM_025273747.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025273747.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGCGCT CCGTGCTGCA GCCTAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGGC TTGTGGAGAC
    CCCAAGGCAA AACCATCCTA CCTTATTGAC AAAAACCTGG AATCTGCTGT GAAATTCATA
    GTCAGAAAAT TCCCTGCTGT AGAAACTCGC AACAACAATC AACAGCTTGC ACAACTGCAG
    AAAGAAAAAT CAGAGATTCT GAAAAATCTG GCATTATATT ACTTCACATT TGTAGATGTT
    ATGGAATTTA AGGACCATGT TTGTGAATTG CTGAATACTA TTGATGTTTG CCAAGTCTTC
    TTTGACATTA CTGTGAACTT TGATTTAACA AAGAACTACT TAGATTTGAT CATAACATAT
    ACAACACTAA TGATACTGCT GTCTCGAATT GAAGAAAGGA AGGCAATCAT TGGATTATAC
    AACTATGCTC ATGAGATGAC CCATGGGGCA AGTGACCGAG AATACCCACG CCTTGGTCAG
    ATGATTGTGG ATTATGAAAA CCCTTTAAAG AAAATGATGG AAGAATTTGT GCCCCATAGC
    AAGTCTCTTT CAGATGCACT AATTTCTCTT CAGATGGTGT ATCCTCGAAG GAACCTTTCA
    GCTGACCAGT GGAGAAATGC CCAGTTGCTG AGTCTCATCA GTGCCCCCAG CACAATGCTT
    AATCCCGCAC AGTCCGACAC TATGCCTTGT GAATATCTCT CTTTGGATGC CATGGAAAAA
    TGGATTATCT TTGGCTTTAT TTTGTGCCAT GGGATCCTAA ATACTGATGC AACAGCGCTG
    AACCTTTGGA AGCTAGCTCT TCAAAGTAGC TCTTGCCTCT CTCTTTTTCG GGACGAAGTT
    TTCCACATTC ATAAAGCTGC AGAAGACTTA TTTGTAAACA TTCGAGGCTA TAATAAGCGT
    ATTAATGACA TAAGAGAATG CAAAGAGGCA GCTGTGTCAC ATGCTGGTTC AATGCACAGA
    GAAAGACGCA AGTTTTTAAG GTCTGCACTG AAAGAATTGG CTACTGTCCT CTCTGATCAG
    CCTGGCTTGC TAGGTCCTAA GGCACTTTTT GTTTTTATGG CATTATCCTT TGCCCGTGAT
    GAAATTATCT GGCTACTTCG TCATGCAGAT AACATGCCAA AGAAGAGTGC AGATGACTTT
    ATAGATAAGC ACATTGCTGA ATTAATATTT TATATGGAAG AGCTTAGAGC ACATGTAAGG
    AAATACGGAC CTGTAATGCA GAGATATTAC GTGCAGTACC TTTCTGGCTT TGATGCTGTT
    GTCCTCAATG AGCTTGTGCA GAATCTTTCT GTTTGCCCTG AAGATGAATC AATCATCATG
    TCCTCTTTTG TTAACACTAT GACTTCTCTA AGTGTAAAAC AAGTTGAAGA TGGGGAAGTA
    TTTGATTTCA GAGGAATGAG ATTAGATTGG TTTCGATTAC AGGCATATAC TAGTGTCTCT
    AAGGCTTCAC TTAGCCTTTC AGATCACAGA GAACTTGGAA AGATGATGAA TACAATAATT
    TTTCACACAA AAATGGTAGA TTCCTTGGTG GAAATGTTGG TGGAAACATC AGATCTCTCC
    ATATTTTGTT TTTATAGTCG TGCTTTTGAG AAGATGTTTC AACAGTGTTT GGAGTTACCC
    TCTCAATCAA GATACTCAAT TGCATTTCCA CTACTTTGCA CTCATTTTAT GAGTTGCACA
    CATGAACTAT GTCCAGAAGA GAGACATCAT ATTGGAGATC GCAGTCTTTC CTTATGTAAT
    ATGTTCCTTG ATGAAATGGC CAAACAAGCT CGAAATCTCA TCACTGATAT TTGCACAGAA
    CAGTGTACTC TTAGTGACCA GTTGTTGCCC AAGCATTGTG CCAAAACCAT CAGTCAAGCA
    GTGAATAAGA AGTCAAAAAA ACAGACTGGT AAGAAAGGGG AACCTGAAAG GGAAAAACCA
    GGAGTTGAAA GCATGAGGAA AAACAGGCTG GTCGTGACCA ACCTTGATAA GTTGCACACT
    GCACTTTCCG AGTTGTGTTT CTCTATAAAT TATGTACCAA ACATGGTGGT TTGGGAACAT
    ACCTTTACCC CACGAGAATA TTTGACTTCT CATCTGGAAA TCCGCTTTAC TAAGTCAATT
    GTTGGAATGA CTATGTATAA TCAAGCCACA CAGGAAATTG CAAAACCATC AGAGCTTCTA
    ACAAGTGTAA GAGCATACAT GACTGTTCTC CAGTCAATAG AAAACTATGT GCAAATTGAT
    ATTACAAGAG TATTTAATAA TGTTCTTCTT CAACAAACAC AACATTTAGA CAGTCATGGA
    GAACCAACCA TTACAAGTCT GTACACAAAT TGGTATTTGG AAACTTTGTT AAGACAAGTC
    AGCAATGGTC ATATAGCTTA TTTCCCTGCA ATGAAAGCAT TTGTGAACTT ACCCACGGAA
    AATGAATTAA CGTTTAATGC AGAGGAATAT TCTGACATAT CAGAAATGAG GTCATTATCA
    GAGCTGCTTG GCCCATATGG TATGAAGTTC CTAAGTGAAA GTCTTATGTG GCATATTTCA
    TCACAAGTTG CTGAACTTAA GAAACTTGTG GTGGAGAATG TTGATGTGCT AACACAAATG
    CGGACCAGCT TTGACAAACC CGACCAGATG GCTGCCCTCT TTAAAAGATT ATCGTCTGTT
    GACAGTGTCT TGAAGAGGAT GACAATAATT GGTGTAATTT TATCTTTCCG ATCACTGGCA
    CAGGAAGCAC TAAGAGATGT CTTGTCCTAC CACATTCCTT TTCTTGTCAG TTCAATTGAA
    GATTTCAAGG ATCACATTCC AAGGGAAACG GATATGAAGG TTGCAATGAA TGTGTATGAA
    TTATCATCAG CTGCTGGATT ACCTTGTGAG ATTGATCCTG CCTTGGTGGT AGCTCTTTCC
    TCACAAAAAT CAGAAAACAT AAGTCCAGAA GAAGAATATA AAATTGCCTG CCTCCTCATG
    GTGTTTGTGG CCGTTTCTTT GCCCACACTG GCCAGTAATG TGATGTCTCA GTATAGCCCT
    GCTATAGAAG GGCACTGCAA CAACATACAT TGCTTGGCCA AAGCCATCAA CCAGATCGCT
    GCAGCTTTGT TTACAATTCA CAAAGGAAGC ATCGAAGATC GGCTTAAAGA ATTTTTGGCG
    CTTGCATCCT CCAGTCTGCT GAAAATTGGC CAGGAGACAG ATAAAACAAC TACACGAAAT
    AGAGAATCTG TTTATTTACT GCTAGATATG ATTGTGCAGG AATCCCCATT CCTGACAATG
    GATCTTTTGG AATCTTGTTT TCCTTATGTC TTGCTGAGAA ATGCATACCA TGCTGTCTAC
    AAGCAAAGTG TTACATCCTC TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu55347
    Clone ID Related Accession (Same CDS sequence) XM_025273748.1
    Accession Version XM_025273748.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3348bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product nck-associated protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCGCGCT CCGTGCTGCA GCCTAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCTT GTGGAGACCC CAAGGCAAAA CCATCCTACC TTATTGACAA AAACCTGGAA
    TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACTCGCAA CAACAATCAA
    CAGCTTGCAC AACTGCAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC
    TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT
    GATGTTTGCC AAGTCTTCTT TGACATTACT GTGAACTTTG ATTTAACAAA GAACTACTTA
    GATTTGATCA TAACATATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG
    GCAATCATTG GATTATACAA CTATGCTCAT GAGATGACCC ATGGGGCAAG TGACCGAGAA
    TACCCACGCC TTGGTCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA AATGATGGAA
    GAATTTGTGC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA GATGGTGTAT
    CCTCGAAGGA ACCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTGCTGAG TCTCATCAGT
    GCCCCCAGCA CAATGCTTAA TCCCGCACAG TCCGACACTA TGCCTTGTGA ATATCTCTCT
    TTGGATGCCA TGGAAAAATG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT
    ACTGATGCAA CAGCGCTGAA CCTTTGGAAG CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT
    CTTTTTCGGG ACGAAGTTTT CCACATTCAT AAAGCTGCAG AAGACTTATT TGTAAACATT
    CGAGGTGGTT CAATGCACAG AGAAAGACGC AAGTTTTTAA GGTCTGCACT GAAAGAATTG
    GCTACTGTCC TCTCTGATCA GCCTGGCTTG CTAGGTCCTA AGGCACTTTT TGTTTTTATG
    GCATTATCCT TTGCCCGTGA TGAAATTATC TGGCTACTTC GTCATGCAGA TAACATGCCA
    AAGAAGAGTG CAGATGACTT TATAGATAAG CACATTGCTG AATTAATATT TTATATGGAA
    GAGCTTAGAG CACATGTAAG GAAATACGGA CCTGTAATGC AGAGATATTA CGTGCAGTAC
    CTTTCTGGCT TTGATGCTGT TGTCCTCAAT GAGCTTGTGC AGAATCTTTC TGTTTGCCCT
    GAAGATGAAT CAATCATCAT GTCCTCTTTT GTTAACACTA TGACTTCTCT AAGTGTAAAA
    CAAGTTGAAG ATGGGGAAGT ATTTGATTTC AGAGGAATGA GATTAGATTG GTTTCGATTA
    CAGGCATATA CTAGTGTCTC TAAGGCTTCA CTTAGCCTTT CAGATCACAG AGAACTTGGA
    AAGATGATGA ATACAATAAT TTTTCACACA AAAATGGTAG ATTCCTTGGT GGAAATGTTG
    GTGGAAACAT CAGATCTCTC CATATTTTGT TTTTATAGTC GTGCTTTTGA GAAGATGTTT
    CAACAGTGTT TGGAGTTACC CTCTCAATCA AGATACTCAA TTGCATTTCC ACTACTTTGC
    ACTCATTTTA TGAGTTGCAC ACATGAACTA TGTCCAGAAG AGAGACATCA TATTGGAGAT
    CGCAGTCTTT CCTTATGTAA TATGTTCCTT GATGAAATGG CCAAACAAGC TCGAAATCTC
    ATCACTGATA TTTGCACAGA ACAGTGTACT CTTAGTGACC AGTTGTTGCC CAAGCATTGT
    GCCAAAACCA TCAGTCAAGC AGTGAATAAG AAGTCAAAAA AACAGACTGG TAAGAAAGGG
    GAACCTGAAA GGGAAAAACC AGGAGTTGAA AGCATGAGGA AAAACAGGCT GGTCGTGACC
    AACCTTGATA AGTTGCACAC TGCACTTTCC GAGTTGTGTT TCTCTATAAA TTATGTACCA
    AACATGGTGG TTTGGGAACA TACCTTTACC CCACGAGAAT ATTTGACTTC TCATCTGGAA
    ATCCGCTTTA CTAAGTCAAT TGTTGGAATG ACTATGTATA ATCAAGCCAC ACAGGAAATT
    GCAAAACCAT CAGAGCTTCT AACAAGTGTA AGAGCATACA TGACTGTTCT CCAGTCAATA
    GAAAACTATG TGCAAATTGA TATTACAAGA GTATTTAATA ATGTTCTTCT TCAACAAACA
    CAACATTTAG ACAGTCATGG AGAACCAACC ATTACAAGTC TGTACACAAA TTGGTATTTG
    GAAACTTTGT TAAGACAAGT CAGCAATGGT CATATAGCTT ATTTCCCTGC AATGAAAGCA
    TTTGTGAACT TACCCACGGA AAATGAATTA ACGTTTAATG CAGAGGAATA TTCTGACATA
    TCAGAAATGA GGTCATTATC AGAGCTGCTT GGCCCATATG GTATGAAGTT CCTAAGTGAA
    AGTCTTATGT GGCATATTTC ATCACAAGTT GCTGAACTTA AGAAACTTGT GGTGGAGAAT
    GTTGATGTGC TAACACAAAT GCGGACCAGC TTTGACAAAC CCGACCAGAT GGCTGCCCTC
    TTTAAAAGAT TATCGTCTGT TGACAGTGTC TTGAAGAGGA TGACAATAAT TGGTGTAATT
    TTATCTTTCC GATCACTGGC ACAGGAAGCA CTAAGAGATG TCTTGTCCTA CCACATTCCT
    TTTCTTGTCA GTTCAATTGA AGATTTCAAG GATCACATTC CAAGGGAAAC GGATATGAAG
    GTTGCAATGA ATGTGTATGA ATTATCATCA GCTGCTGGAT TACCTTGTGA GATTGATCCT
    GCCTTGGTGG TAGCTCTTTC CTCACAAAAA TCAGAAAACA TAAGTCCAGA AGAAGAATAT
    AAAATTGCCT GCCTCCTCAT GGTGTTTGTG GCCGTTTCTT TGCCCACACT GGCCAGTAAT
    GTGATGTCTC AGTATAGCCC TGCTATAGAA GGGCACTGCA ACAACATACA TTGCTTGGCC
    AAAGCCATCA ACCAGATCGC TGCAGCTTTG TTTACAATTC ACAAAGGAAG CATCGAAGAT
    CGGCTTAAAG AATTTTTGGC GCTTGCATCC TCCAGTCTGC TGAAAATTGG CCAGGAGACA
    GATAAAACAA CTACACGAAA TAGAGAATCT GTTTATTTAC TGCTAGATAT GATTGTGCAG
    GAATCCCCAT TCCTGACAAT GGATCTTTTG GAATCTTGTT TTCCTTATGT CTTGCTGAGA
    AATGCATACC ATGCTGTCTA CAAGCAAAGT GTTACATCCT CTGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025129533.1
    CDS337..3684
    Translation

    Target ORF information:

    RefSeq Version XM_025273748.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NCK associated protein 1 (NCKAP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025273748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCGCGCT CCGTGCTGCA GCCTAGTCAG CAGAAGCTGG CGGAGAAGCT CACCATCCTC 
    AACGACCGGG GCGTCGGCAT GCTCACCCGC CTCTACAACA TCAAGAAGCA AGGACAAGTT
    TGGAAGGCTT GTGGAGACCC CAAGGCAAAA CCATCCTACC TTATTGACAA AAACCTGGAA
    TCTGCTGTGA AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACTCGCAA CAACAATCAA
    CAGCTTGCAC AACTGCAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC
    TTCACATTTG TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT
    GATGTTTGCC AAGTCTTCTT TGACATTACT GTGAACTTTG ATTTAACAAA GAACTACTTA
    GATTTGATCA TAACATATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG
    GCAATCATTG GATTATACAA CTATGCTCAT GAGATGACCC ATGGGGCAAG TGACCGAGAA
    TACCCACGCC TTGGTCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA AATGATGGAA
    GAATTTGTGC CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA GATGGTGTAT
    CCTCGAAGGA ACCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTGCTGAG TCTCATCAGT
    GCCCCCAGCA CAATGCTTAA TCCCGCACAG TCCGACACTA TGCCTTGTGA ATATCTCTCT
    TTGGATGCCA TGGAAAAATG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT
    ACTGATGCAA CAGCGCTGAA CCTTTGGAAG CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT
    CTTTTTCGGG ACGAAGTTTT CCACATTCAT AAAGCTGCAG AAGACTTATT TGTAAACATT
    CGAGGTGGTT CAATGCACAG AGAAAGACGC AAGTTTTTAA GGTCTGCACT GAAAGAATTG
    GCTACTGTCC TCTCTGATCA GCCTGGCTTG CTAGGTCCTA AGGCACTTTT TGTTTTTATG
    GCATTATCCT TTGCCCGTGA TGAAATTATC TGGCTACTTC GTCATGCAGA TAACATGCCA
    AAGAAGAGTG CAGATGACTT TATAGATAAG CACATTGCTG AATTAATATT TTATATGGAA
    GAGCTTAGAG CACATGTAAG GAAATACGGA CCTGTAATGC AGAGATATTA CGTGCAGTAC
    CTTTCTGGCT TTGATGCTGT TGTCCTCAAT GAGCTTGTGC AGAATCTTTC TGTTTGCCCT
    GAAGATGAAT CAATCATCAT GTCCTCTTTT GTTAACACTA TGACTTCTCT AAGTGTAAAA
    CAAGTTGAAG ATGGGGAAGT ATTTGATTTC AGAGGAATGA GATTAGATTG GTTTCGATTA
    CAGGCATATA CTAGTGTCTC TAAGGCTTCA CTTAGCCTTT CAGATCACAG AGAACTTGGA
    AAGATGATGA ATACAATAAT TTTTCACACA AAAATGGTAG ATTCCTTGGT GGAAATGTTG
    GTGGAAACAT CAGATCTCTC CATATTTTGT TTTTATAGTC GTGCTTTTGA GAAGATGTTT
    CAACAGTGTT TGGAGTTACC CTCTCAATCA AGATACTCAA TTGCATTTCC ACTACTTTGC
    ACTCATTTTA TGAGTTGCAC ACATGAACTA TGTCCAGAAG AGAGACATCA TATTGGAGAT
    CGCAGTCTTT CCTTATGTAA TATGTTCCTT GATGAAATGG CCAAACAAGC TCGAAATCTC
    ATCACTGATA TTTGCACAGA ACAGTGTACT CTTAGTGACC AGTTGTTGCC CAAGCATTGT
    GCCAAAACCA TCAGTCAAGC AGTGAATAAG AAGTCAAAAA AACAGACTGG TAAGAAAGGG
    GAACCTGAAA GGGAAAAACC AGGAGTTGAA AGCATGAGGA AAAACAGGCT GGTCGTGACC
    AACCTTGATA AGTTGCACAC TGCACTTTCC GAGTTGTGTT TCTCTATAAA TTATGTACCA
    AACATGGTGG TTTGGGAACA TACCTTTACC CCACGAGAAT ATTTGACTTC TCATCTGGAA
    ATCCGCTTTA CTAAGTCAAT TGTTGGAATG ACTATGTATA ATCAAGCCAC ACAGGAAATT
    GCAAAACCAT CAGAGCTTCT AACAAGTGTA AGAGCATACA TGACTGTTCT CCAGTCAATA
    GAAAACTATG TGCAAATTGA TATTACAAGA GTATTTAATA ATGTTCTTCT TCAACAAACA
    CAACATTTAG ACAGTCATGG AGAACCAACC ATTACAAGTC TGTACACAAA TTGGTATTTG
    GAAACTTTGT TAAGACAAGT CAGCAATGGT CATATAGCTT ATTTCCCTGC AATGAAAGCA
    TTTGTGAACT TACCCACGGA AAATGAATTA ACGTTTAATG CAGAGGAATA TTCTGACATA
    TCAGAAATGA GGTCATTATC AGAGCTGCTT GGCCCATATG GTATGAAGTT CCTAAGTGAA
    AGTCTTATGT GGCATATTTC ATCACAAGTT GCTGAACTTA AGAAACTTGT GGTGGAGAAT
    GTTGATGTGC TAACACAAAT GCGGACCAGC TTTGACAAAC CCGACCAGAT GGCTGCCCTC
    TTTAAAAGAT TATCGTCTGT TGACAGTGTC TTGAAGAGGA TGACAATAAT TGGTGTAATT
    TTATCTTTCC GATCACTGGC ACAGGAAGCA CTAAGAGATG TCTTGTCCTA CCACATTCCT
    TTTCTTGTCA GTTCAATTGA AGATTTCAAG GATCACATTC CAAGGGAAAC GGATATGAAG
    GTTGCAATGA ATGTGTATGA ATTATCATCA GCTGCTGGAT TACCTTGTGA GATTGATCCT
    GCCTTGGTGG TAGCTCTTTC CTCACAAAAA TCAGAAAACA TAAGTCCAGA AGAAGAATAT
    AAAATTGCCT GCCTCCTCAT GGTGTTTGTG GCCGTTTCTT TGCCCACACT GGCCAGTAAT
    GTGATGTCTC AGTATAGCCC TGCTATAGAA GGGCACTGCA ACAACATACA TTGCTTGGCC
    AAAGCCATCA ACCAGATCGC TGCAGCTTTG TTTACAATTC ACAAAGGAAG CATCGAAGAT
    CGGCTTAAAG AATTTTTGGC GCTTGCATCC TCCAGTCTGC TGAAAATTGG CCAGGAGACA
    GATAAAACAA CTACACGAAA TAGAGAATCT GTTTATTTAC TGCTAGATAT GATTGTGCAG
    GAATCCCCAT TCCTGACAAT GGATCTTTTG GAATCTTGTT TTCCTTATGT CTTGCTGAGA
    AATGCATACC ATGCTGTCTA CAAGCAAAGT GTTACATCCT CTGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.