HLCS cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	HLCS
Entrez Gene ID	102407054
Full Name	holocarboxylase synthetase
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025293760.1	XP_025149545.1	biotin--protein ligase isoform X1
XM_006044088.1	XP_006044150.1	biotin--protein ligase
XM_025293769.1	XP_025149554.1	biotin--protein ligase isoform X2
XM_025293775.1	XP_025149560.1	biotin--protein ligase isoform X3
XM_025293779.1	XP_025149564.1	biotin--protein ligase isoform X4

Pathways from BioSystems
Gene Ontology

The following HLCS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLCS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu03318	XM_006044088.1 Latest version!	Bubalus bubalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu43465	XM_025293760.1 Latest version!	Bubalus bubalis holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu43466	XM_025293769.1 Latest version!	Bubalus bubalis holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu43467	XM_025293775.1 Latest version!	Bubalus bubalis holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBu43468	XM_025293779.1 Latest version!	Bubalus bubalis holocarboxylase synthetase (HLCS), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HLCS ( XM_006044088.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044150.1 Bubalus bubalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), mRNA.

Price & Availability↑

CloneID	OBu03318
Clone ID Related Accession (Same CDS sequence)	XM_006044088.1
Accession Version	XM_006044088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	biotin--protein ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783537.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTGA GAAGATTTTG GGAAATTGTG AAGTGGTCTG ACTGCTGTTT GCCGTTAGCT 
TGCCGACCTG GGGATCCTTA TCAATTAATC GCCAAAGCAA GTGTGGATGA CTTCAGCAAG 
CTAGGGGTGG CATTCCTGGA AGATAGACTC CAGATGGCTA ATGGACTGAT ACCCCAGAAG 
ATTGTGTCTG TCCACTTGCA GGACTCTGCC CTAAAGGAAC TTAAGGGTCA GGCCTCAGAC 
CCGCCAGCCC AGCTCCTGCA GCCTGACCCT GAATCTTGTA TTGAGAGGAA CCCTCCTGAG 
CCCGACACTA TGGAGGACCC CCAGTCTCCG GGTTCTGCAT CCACACGGGG GCCAGGCGGG 
GCCTCTGGGG GCAGTGAGCC AGCCGTAGAC GGTGACAGAG GCGTGGGCTC TGTCGAGCAC 
TGTCCCCTCC ATCTGTCCAG CTGCCATGAG TGTTTGGAGC TTGAGAACAG CACCATTGAA 
TCTGTCAGGT TTGCCTCTGC AGAGAACATT CCAGATCTCC CGTACGACTA CAGCAGCGGT 
TTGGAGGGCC TCGCCGACGA CCTCCGCCCC GAGAGGGATG AGAGGAGGGT CAATGTCACG 
GGCAAGGCCC CCAACATCCT CCTTTACGTG GGCTCTGACT CTCGGGATGG CCAGGACAGG 
CTCCAGCAGG TCCAATCTGC GCTGGCCGAC TGTGTGGACA CGGACTGTTA TACTCTGTAC 
CACCTGCCTC CAGAAAGCGC TCTCAGGGAC CCGTGGCCGG ACAACTGTCT GCTGCTGGTC 
ATCGCCACCG GGGAGCCCGT CCCCGAGGAC CTGTCCCGGA GGTTCACGGC CTATCTTTCT 
CAGGGAGGGA AGGTGCTGGC CCTGGCGTCG TCCTTCACGC TGCCTGGCTT CCAGGTGACC 
AGCAAGGCCC CGCTGCAGGA CACGGTCCAG AGCTTGGTTT TCTGCAAGGT GGACGGAAGC 
CTGATGAGGC TCAGTGTCCT GAGCAGCGGC TGCGTTTACG AGGAGGGGCC CAGGGAGCCG 
CTCGGCCCCG GCAAGCTCCA CGGCCACCTG GACAGTGAGG ATGGGGACAG GCTTGTGGTG 
CAAGTGCCTT TTGGGACTCA CGGAGGAGAA GCTGTTCTTT GCCAGGTGCA CTTGGAACTA 
CCTCCCAGCT CTCCAGTAGT GCAAACACAA GAGGATTTTA ACCTGCTTAA ATCAAGCAAC 
TTTAGAAGAT ACGAAGTCCT TACGGAGATC CTAACGACGC TGGGCCTAAG CTGTGACGCA 
AAGCAAGCTC CTGCCTTAAC GCCTCTTTAC CTGCTGACGG CGACCGAGGA AATCCGGGAT 
CCTCTTGTGC AGTGGCTGGG GAAACATGTG GATCCTGAAG GAGTAATAAG ATCGAGCAAA 
CTCTCCCTGA AATTCATTTC ATCCTACACA TCTGAAATAG AAATCACCCC ATCCGCCCTA 
CCCGTGGTGA CTGACACAGA GTCCTTCTCA TCAGAAAACT TTGACTTCGA GATTTACCGA 
CAAAACCTGA AGACAAAGAA ACTTGGGAAA GTCATTCTGT TTGCCGAAGT GACACCCACT 
ACAATGAATC TTCTGGACGG GTTGATGTTT GAGATGCCGC AGGAAATGGG TTTAATAGCC 
ATCGCAGTCC GCCAGACGCA GGGCAAAGGT CAGTCATCTT TTGTTACTGT TTCTGTTGAC 
ATCAATTTGC GAGTGAAGTG GCCCAATGAT ATTTATTACA GTGACCTCAT GAAGCTTGGT 
GGAGTTTTGG TTAACTCAAC ACTTATGGGA GAAACATTCT ATATACTTAT TGGCTGTGGA 
TTTAATGTGA CAAACAGCAA CCCTACCATA TGCATCAATG ATCTCATCAC AGAATACAAT 
AAGGAACACG GGGCAGAGCT ACAGCCCCTG AGAGCCGACT GTCTCATCGC CCGAACAGTG 
ACCGTGCTGG AGAAGCTGAT TGACGTGTTT CAGGACCAGG GGCCCAACGG TGTTCTTCCT 
CTCTACTATA AATACTGGGT ACACAGCGCT CAGCAAGTCC GCCTGGGAAG CGCTGAGGGA 
CCAAAGGTGT GGATAGTTGG CCTGGATGAT TCTGGGTTCC TTCTGGTCCA CCAGGAAAAC 
GGCGATGTGG TCACGGTGCA TCCTGACGGC AACTCCTTCG ACATGCTGAG AAACCTCATC 
GTCCCCAAAC AGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044150.1
CDS	1..2178
Translation	MFLRRFWEIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESCIERNPPEPDTMEDPQSPGSASTRGPGGASGGSEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSSGLEGLADDLRPERDERRVNVTGKAPNILLYVGSDSRDGQDRLQQVQSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLMRLSVLSSGCVYEEGPREPLGPGKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPVVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDAKQAPALTPLYLLTATEEIRDPLVQWLGKHVDPEGVIRSSKLSLKFISSYTSEIEITPSALPVVTDTESFSSENFDFEIYRQNLKTKKLGKVILFAEVTPTTMNLLDGLMFEMPQEMGLIAIAVRQTQGKGQSSFVTVSVDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTVTVLEKLIDVFQDQGPNGVLPLYYKYWVHSAQQVRLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_006044088.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044088.1

ORF Insert Sequence:

ATGTTCCTGA GAAGATTTTG GGAAATTGTG AAGTGGTCTG ACTGCTGTTT GCCGTTAGCT 
TGCCGACCTG GGGATCCTTA TCAATTAATC GCCAAAGCAA GTGTGGATGA CTTCAGCAAG 
CTAGGGGTGG CATTCCTGGA AGATAGACTC CAGATGGCTA ATGGACTGAT ACCCCAGAAG 
ATTGTGTCTG TCCACTTGCA GGACTCTGCC CTAAAGGAAC TTAAGGGTCA GGCCTCAGAC 
CCGCCAGCCC AGCTCCTGCA GCCTGACCCT GAATCTTGTA TTGAGAGGAA CCCTCCTGAG 
CCCGACACTA TGGAGGACCC CCAGTCTCCG GGTTCTGCAT CCACACGGGG GCCAGGCGGG 
GCCTCTGGGG GCAGTGAGCC AGCCGTAGAC GGTGACAGAG GCGTGGGCTC TGTCGAGCAC 
TGTCCCCTCC ATCTGTCCAG CTGCCATGAG TGTTTGGAGC TTGAGAACAG CACCATTGAA 
TCTGTCAGGT TTGCCTCTGC AGAGAACATT CCAGATCTCC CGTACGACTA CAGCAGCGGT 
TTGGAGGGCC TCGCCGACGA CCTCCGCCCC GAGAGGGATG AGAGGAGGGT CAATGTCACG 
GGCAAGGCCC CCAACATCCT CCTTTACGTG GGCTCTGACT CTCGGGATGG CCAGGACAGG 
CTCCAGCAGG TCCAATCTGC GCTGGCCGAC TGTGTGGACA CGGACTGTTA TACTCTGTAC 
CACCTGCCTC CAGAAAGCGC TCTCAGGGAC CCGTGGCCGG ACAACTGTCT GCTGCTGGTC 
ATCGCCACCG GGGAGCCCGT CCCCGAGGAC CTGTCCCGGA GGTTCACGGC CTATCTTTCT 
CAGGGAGGGA AGGTGCTGGC CCTGGCGTCG TCCTTCACGC TGCCTGGCTT CCAGGTGACC 
AGCAAGGCCC CGCTGCAGGA CACGGTCCAG AGCTTGGTTT TCTGCAAGGT GGACGGAAGC 
CTGATGAGGC TCAGTGTCCT GAGCAGCGGC TGCGTTTACG AGGAGGGGCC CAGGGAGCCG 
CTCGGCCCCG GCAAGCTCCA CGGCCACCTG GACAGTGAGG ATGGGGACAG GCTTGTGGTG 
CAAGTGCCTT TTGGGACTCA CGGAGGAGAA GCTGTTCTTT GCCAGGTGCA CTTGGAACTA 
CCTCCCAGCT CTCCAGTAGT GCAAACACAA GAGGATTTTA ACCTGCTTAA ATCAAGCAAC 
TTTAGAAGAT ACGAAGTCCT TACGGAGATC CTAACGACGC TGGGCCTAAG CTGTGACGCA 
AAGCAAGCTC CTGCCTTAAC GCCTCTTTAC CTGCTGACGG CGACCGAGGA AATCCGGGAT 
CCTCTTGTGC AGTGGCTGGG GAAACATGTG GATCCTGAAG GAGTAATAAG ATCGAGCAAA 
CTCTCCCTGA AATTCATTTC ATCCTACACA TCTGAAATAG AAATCACCCC ATCCGCCCTA 
CCCGTGGTGA CTGACACAGA GTCCTTCTCA TCAGAAAACT TTGACTTCGA GATTTACCGA 
CAAAACCTGA AGACAAAGAA ACTTGGGAAA GTCATTCTGT TTGCCGAAGT GACACCCACT 
ACAATGAATC TTCTGGACGG GTTGATGTTT GAGATGCCGC AGGAAATGGG TTTAATAGCC 
ATCGCAGTCC GCCAGACGCA GGGCAAAGGT CAGTCATCTT TTGTTACTGT TTCTGTTGAC 
ATCAATTTGC GAGTGAAGTG GCCCAATGAT ATTTATTACA GTGACCTCAT GAAGCTTGGT 
GGAGTTTTGG TTAACTCAAC ACTTATGGGA GAAACATTCT ATATACTTAT TGGCTGTGGA 
TTTAATGTGA CAAACAGCAA CCCTACCATA TGCATCAATG ATCTCATCAC AGAATACAAT 
AAGGAACACG GGGCAGAGCT ACAGCCCCTG AGAGCCGACT GTCTCATCGC CCGAACAGTG 
ACCGTGCTGG AGAAGCTGAT TGACGTGTTT CAGGACCAGG GGCCCAACGG TGTTCTTCCT 
CTCTACTATA AATACTGGGT ACACAGCGCT CAGCAAGTCC GCCTGGGAAG CGCTGAGGGA 
CCAAAGGTGT GGATAGTTGG CCTGGATGAT TCTGGGTTCC TTCTGGTCCA CCAGGAAAAC 
GGCGATGTGG TCACGGTGCA TCCTGACGGC AACTCCTTCG ACATGCTGAG AAACCTCATC 
GTCCCCAAAC AGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_025293760.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025149545.1 Bubalus bubalis holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu43465
Clone ID Related Accession (Same CDS sequence)	XM_025293760.1
Accession Version	XM_025293760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC 
GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTGAGCC GTGGCGGCCG GGTCTTCTGT 
GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTTTCTCCT 
TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG 
AATTTACAGA CACCGGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG 
CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC 
TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA 
CCCCAGAAGA TTGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG 
GCCTCAGACC CGCCAGCCCA GCTCCTGCAG CCTGACCCTG AATCTTGTAT TGAGAGGAAC 
CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCGG GTTCTGCATC CACACGGGGG 
CCAGGCGGGG CCTCTGGGGG CAGCGAGCCA GCCGTAGACG GTGACAGAGG CGTGGGCTCT 
GTCGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCATGAGT GTTTGGAGCT TGAGAACAGC 
ACCATTGAAT CTGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC 
AGCAGCGGTT TGGAGGGCCT CGCCGACGAC CTCCGCCCCG AGAGGGATGA GAGGAGGGTC 
AATGTCACGG GCAAGGCCCC CAACATCCTC CTTTACGTGG GCTCTGACTC TCGGGATGGC 
CAGGACAGGC TCCAGCAGGT CCAATCTGCG CTGGCCGACT GTGTGGACAC GGACTGTTAT 
ACTCTGTACC ACCTGCCTCC AGAAAGCGCT CTCAGGGACC CGTGGCCGGA CAACTGTCTG 
CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCCGAGGACC TGTCCCGGAG GTTCACGGCC 
TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCGTCAT CCTTCACGCT GCCTGGCTTC 
CAGGTGACCA GCAAGGCCCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGTG 
GACGGAAGCC TGATGAGGCT CAGTGTCCTG AGCAGCGGCT GCGTTTACGA GGAGGGGCCC 
AGGGAGCCGC TCGGCCCCGG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA TGGGGACAGG 
CTTGTGGTGC AAGTGCCTTT TGGGACTCAC GGAGGAGAAG CTGTTCTTTG CCAGGTGCAC 
TTGGAACTAC CTCCCAGCTC TCCAGTAGTG CAAACACAAG AGGATTTTAA CCTGCTTAAA 
TCAAGCAACT TTAGAAGATA CGAAGTCCTT ACGGAGATCC TAACGACGCT GGGCCTAAGC 
TGTGACGCAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGACGGC GACCGAGGAA 
ATCCGGGATC CTCTTGTGCA GTGGCTGGGG AAACATGTGG ATCCTGAAGG AGTAATAAGA 
TCGAGCAAAC TCTCCCTGAA ATTCATTTCA TCCTATACAT CTGAAATAGA AATCACCCCA 
TCCGCCCTAC CCGTGGTGAC TGACACAGAG TCCTTCTCAT CAGAAAACTT TGACTTCGAG 
ATTTACCGAC AAAACCTGAA GACAAAGAAA CTTGGGAAAG TCATTCTGTT TGCCGAAGTG 
ACACCCACTA CAATGAATCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT 
TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG 
AGCCCCTTGG GATGCGCCCT TTCCACCCTG CTGGTCTGCA TCCCGCTGAG ATCCCCGCTG 
GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCAGTAAGA 
TCCATCCCCG GGTACCAGGA CATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC 
AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCAA CACTTATGGG AGAAACATTC 
TATATACTTA TTGGCTGTGG ATTTAATGTG ACAAACAGCA ACCCTACCAT ATGCATCAAT 
GATCTCATCA CAGAATACAA TAAGGAACAC GGGGCAGAGC TACAGCCCCT GAGAGCCGAC 
TGTCTCATCG CCCGAACAGT GACCGTGCTG GAGAAGCTGA TTGACGTGTT TCAGGACCAG 
GGGCCCAACG GTGTTCTTCC TCTCTACTAT AAATACTGGG TACACAGCGC TCAGCAAGTC 
CACCTGGGAA GCGCTGAGGG ACCAAAGGTG TGGATAGTTG GCCTGGATGA TTCTGGGTTC 
CTTCTGGTCC ACCAGGAAAA CGGCGATGTG GTCACGGTGC ATCCTGACGG CAACTCCTTT 
GACATGCTGA GAAACCTCAT CGTCCCCAAA CAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025149545.1
CDS	34..2652
Translation	MLITLCYLYLWARWGRGPAALVRATVQRLRASRCSFTFCSAAAPPRGSRVCLSRGGRVFCVGESQSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQTPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESCIERNPPEPDTMEDPQSPGSASTRGPGGASGGSEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSSGLEGLADDLRPERDERRVNVTGKAPNILLYVGSDSRDGQDRLQQVQSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLMRLSVLSSGCVYEEGPREPLGPGKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPVVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDAKQAPALTPLYLLTATEEIRDPLVQWLGKHVDPEGVIRSSKLSLKFISSYTSEIEITPSALPVVTDTESFSSENFDFEIYRQNLKTKKLGKVILFAEVTPTTMNLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTVTVLEKLIDVFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_025293760.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis holocarboxylase synthetase (HLCS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025293760.1

ORF Insert Sequence:

ATGCTCATCA CGCTGTGCTA CCTGTACCTG TGGGCGCGCT GGGGGCGCGG GCCGGCCGCG 
CTCGTGCGCG CCACGGTGCA GCGGCTGCGC GCCTCGCGCT GCTCCTTCAC CTTCTGCAGC 
GCGGCCGCAC CGCCCCGGGG CTCCCGCGTG TGCCTGAGCC GTGGCGGCCG GGTCTTCTGT 
GTCGGCGAGA GCCAGTCCAT TGATGACTTA AACAAATGGG CCCTGTTCCT GGTTTCTCCT 
TTTATACTTG AAGCAGAGCA CATCGCCTTT GTGACAGAGA GCATTTGGGT GCAAGGTGAG 
AATTTACAGA CACCGGCATC CTCTTCGGAA ACTATTGTGA AGTGGTCTGA CTGCTGTTTG 
CCGTTAGCTT GCCGACCTGG GGATCCTTAT CAATTAATCG CCAAAGCAAG TGTGGATGAC 
TTCAGCAAGC TAGGGGTGGC ATTCCTGGAA GATAGACTCC AGATGGCTAA TGGACTGATA 
CCCCAGAAGA TTGTGTCTGT CCACTTGCAG GACTCTGCCC TAAAGGAACT TAAGGGTCAG 
GCCTCAGACC CGCCAGCCCA GCTCCTGCAG CCTGACCCTG AATCTTGTAT TGAGAGGAAC 
CCTCCTGAGC CCGACACTAT GGAGGACCCC CAGTCTCCGG GTTCTGCATC CACACGGGGG 
CCAGGCGGGG CCTCTGGGGG CAGCGAGCCA GCCGTAGACG GTGACAGAGG CGTGGGCTCT 
GTCGAGCACT GTCCCCTCCA TCTGTCCAGC TGCCATGAGT GTTTGGAGCT TGAGAACAGC 
ACCATTGAAT CTGTCAGGTT TGCCTCTGCA GAGAACATTC CAGATCTCCC GTACGACTAC 
AGCAGCGGTT TGGAGGGCCT CGCCGACGAC CTCCGCCCCG AGAGGGATGA GAGGAGGGTC 
AATGTCACGG GCAAGGCCCC CAACATCCTC CTTTACGTGG GCTCTGACTC TCGGGATGGC 
CAGGACAGGC TCCAGCAGGT CCAATCTGCG CTGGCCGACT GTGTGGACAC GGACTGTTAT 
ACTCTGTACC ACCTGCCTCC AGAAAGCGCT CTCAGGGACC CGTGGCCGGA CAACTGTCTG 
CTGCTGGTCA TCGCCACCGG GGAGCCCGTC CCCGAGGACC TGTCCCGGAG GTTCACGGCC 
TATCTTTCTC AGGGAGGGAA GGTGCTGGCC CTGGCGTCAT CCTTCACGCT GCCTGGCTTC 
CAGGTGACCA GCAAGGCCCC GCTGCAGGAC ACGGTCCAGA GCTTGGTTTT CTGCAAGGTG 
GACGGAAGCC TGATGAGGCT CAGTGTCCTG AGCAGCGGCT GCGTTTACGA GGAGGGGCCC 
AGGGAGCCGC TCGGCCCCGG CAAGCTCCAC GGCCACCTGG ACAGTGAGGA TGGGGACAGG 
CTTGTGGTGC AAGTGCCTTT TGGGACTCAC GGAGGAGAAG CTGTTCTTTG CCAGGTGCAC 
TTGGAACTAC CTCCCAGCTC TCCAGTAGTG CAAACACAAG AGGATTTTAA CCTGCTTAAA 
TCAAGCAACT TTAGAAGATA CGAAGTCCTT ACGGAGATCC TAACGACGCT GGGCCTAAGC 
TGTGACGCAA AGCAAGCTCC TGCCTTAACG CCTCTTTACC TGCTGACGGC GACCGAGGAA 
ATCCGGGATC CTCTTGTGCA GTGGCTGGGG AAACATGTGG ATCCTGAAGG AGTAATAAGA 
TCGAGCAAAC TCTCCCTGAA ATTCATTTCA TCCTATACAT CTGAAATAGA AATCACCCCA 
TCCGCCCTAC CCGTGGTGAC TGACACAGAG TCCTTCTCAT CAGAAAACTT TGACTTCGAG 
ATTTACCGAC AAAACCTGAA GACAAAGAAA CTTGGGAAAG TCATTCTGTT TGCCGAAGTG 
ACACCCACTA CAATGAATCT TCTGGACGGG TTGATGTTTG AGATGCCGCA GGAAATGGGT 
TTAATAGCCA TCGCAGTCCG CCAGACGCAG GGCAAAGGGC GGGGAAAGAA TGCTTGGCTG 
AGCCCCTTGG GATGCGCCCT TTCCACCCTG CTGGTCTGCA TCCCGCTGAG ATCCCCGCTG 
GGACAGAGGA TTCCGTTTGT CCAGCATCTG ATGTCCCTGG CCGTCGTAGA GGCAGTAAGA 
TCCATCCCCG GGTACCAGGA CATCAATTTG CGAGTGAAGT GGCCCAATGA TATTTATTAC 
AGTGACCTCA TGAAGCTTGG TGGAGTTTTG GTTAACTCAA CACTTATGGG AGAAACATTC 
TATATACTTA TTGGCTGTGG ATTTAATGTG ACAAACAGCA ACCCTACCAT ATGCATCAAT 
GATCTCATCA CAGAATACAA TAAGGAACAC GGGGCAGAGC TACAGCCCCT GAGAGCCGAC 
TGTCTCATCG CCCGAACAGT GACCGTGCTG GAGAAGCTGA TTGACGTGTT TCAGGACCAG 
GGGCCCAACG GTGTTCTTCC TCTCTACTAT AAATACTGGG TACACAGCGC TCAGCAAGTC 
CACCTGGGAA GCGCTGAGGG ACCAAAGGTG TGGATAGTTG GCCTGGATGA TTCTGGGTTC 
CTTCTGGTCC ACCAGGAAAA CGGCGATGTG GTCACGGTGC ATCCTGACGG CAACTCCTTT 
GACATGCTGA GAAACCTCAT CGTCCCCAAA CAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_025293769.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025149554.1 Bubalus bubalis holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu43466
Clone ID Related Accession (Same CDS sequence)	XM_025293769.1
Accession Version	XM_025293769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGGG ATGGGGGATG GGGGTCCAGA GGAGCCAACC AAAGAGGTTT GGCACTTTCT 
CCTGACCTGC TTCAAAGAAG CCTCTATAAA AACCCTTGGG AAGATCTACC AAGAGGGCAA 
CCCGGAGAGG GCTTGGACCG TCTGCCTCCC CCACCTCCTG CTTCTGAGCA TCTCTCACCG 
GAAACATCCA TTGATGACTT AAACAAATGG GCCCTGTTCC TGGTTTCTCC TTTTATACTT 
GAAGCAGAGC ACATCGCCTT TGTGACAGAG AGCATTTGGG TGCAAGGTGA GAATTTACAG 
ACACCGGCAT CCTCTTCGGA AACTATTGTG AAGTGGTCTG ACTGCTGTTT GCCGTTAGCT 
TGCCGACCTG GGGATCCTTA TCAATTAATC GCCAAAGCAA GTGTGGATGA CTTCAGCAAG 
CTAGGGGTGG CATTCCTGGA AGATAGACTC CAGATGGCTA ATGGACTGAT ACCCCAGAAG 
ATTGTGTCTG TCCACTTGCA GGACTCTGCC CTAAAGGAAC TTAAGGGTCA GGCCTCAGAC 
CCGCCAGCCC AGCTCCTGCA GCCTGACCCT GAATCTTGTA TTGAGAGGAA CCCTCCTGAG 
CCCGACACTA TGGAGGACCC CCAGTCTCCG GGTTCTGCAT CCACACGGGG GCCAGGCGGG 
GCCTCTGGGG GCAGCGAGCC AGCCGTAGAC GGTGACAGAG GCGTGGGCTC TGTCGAGCAC 
TGTCCCCTCC ATCTGTCCAG CTGCCATGAG TGTTTGGAGC TTGAGAACAG CACCATTGAA 
TCTGTCAGGT TTGCCTCTGC AGAGAACATT CCAGATCTCC CGTACGACTA CAGCAGCGGT 
TTGGAGGGCC TCGCCGACGA CCTCCGCCCC GAGAGGGATG AGAGGAGGGT CAATGTCACG 
GGCAAGGCCC CCAACATCCT CCTTTACGTG GGCTCTGACT CTCGGGATGG CCAGGACAGG 
CTCCAGCAGG TCCAATCTGC GCTGGCCGAC TGTGTGGACA CGGACTGTTA TACTCTGTAC 
CACCTGCCTC CAGAAAGCGC TCTCAGGGAC CCGTGGCCGG ACAACTGTCT GCTGCTGGTC 
ATCGCCACCG GGGAGCCCGT CCCCGAGGAC CTGTCCCGGA GGTTCACGGC CTATCTTTCT 
CAGGGAGGGA AGGTGCTGGC CCTGGCGTCA TCCTTCACGC TGCCTGGCTT CCAGGTGACC 
AGCAAGGCCC CGCTGCAGGA CACGGTCCAG AGCTTGGTTT TCTGCAAGGT GGACGGAAGC 
CTGATGAGGC TCAGTGTCCT GAGCAGCGGC TGCGTTTACG AGGAGGGGCC CAGGGAGCCG 
CTCGGCCCCG GCAAGCTCCA CGGCCACCTG GACAGTGAGG ATGGGGACAG GCTTGTGGTG 
CAAGTGCCTT TTGGGACTCA CGGAGGAGAA GCTGTTCTTT GCCAGGTGCA CTTGGAACTA 
CCTCCCAGCT CTCCAGTAGT GCAAACACAA GAGGATTTTA ACCTGCTTAA ATCAAGCAAC 
TTTAGAAGAT ACGAAGTCCT TACGGAGATC CTAACGACGC TGGGCCTAAG CTGTGACGCA 
AAGCAAGCTC CTGCCTTAAC GCCTCTTTAC CTGCTGACGG CGACCGAGGA AATCCGGGAT 
CCTCTTGTGC AGTGGCTGGG GAAACATGTG GATCCTGAAG GAGTAATAAG ATCGAGCAAA 
CTCTCCCTGA AATTCATTTC ATCCTATACA TCTGAAATAG AAATCACCCC ATCCGCCCTA 
CCCGTGGTGA CTGACACAGA GTCCTTCTCA TCAGAAAACT TTGACTTCGA GATTTACCGA 
CAAAACCTGA AGACAAAGAA ACTTGGGAAA GTCATTCTGT TTGCCGAAGT GACACCCACT 
ACAATGAATC TTCTGGACGG GTTGATGTTT GAGATGCCGC AGGAAATGGG TTTAATAGCC 
ATCGCAGTCC GCCAGACGCA GGGCAAAGGG CGGGGAAAGA ATGCTTGGCT GAGCCCCTTG 
GGATGCGCCC TTTCCACCCT GCTGGTCTGC ATCCCGCTGA GATCCCCGCT GGGACAGAGG 
ATTCCGTTTG TCCAGCATCT GATGTCCCTG GCCGTCGTAG AGGCAGTAAG ATCCATCCCC 
GGGTACCAGG ACATCAATTT GCGAGTGAAG TGGCCCAATG ATATTTATTA CAGTGACCTC 
ATGAAGCTTG GTGGAGTTTT GGTTAACTCA ACACTTATGG GAGAAACATT CTATATACTT 
ATTGGCTGTG GATTTAATGT GACAAACAGC AACCCTACCA TATGCATCAA TGATCTCATC 
ACAGAATACA ATAAGGAACA CGGGGCAGAG CTACAGCCCC TGAGAGCCGA CTGTCTCATC 
GCCCGAACAG TGACCGTGCT GGAGAAGCTG ATTGACGTGT TTCAGGACCA GGGGCCCAAC 
GGTGTTCTTC CTCTCTACTA TAAATACTGG GTACACAGCG CTCAGCAAGT CCACCTGGGA 
AGCGCTGAGG GACCAAAGGT GTGGATAGTT GGCCTGGATG ATTCTGGGTT CCTTCTGGTC 
CACCAGGAAA ACGGCGATGT GGTCACGGTG CATCCTGACG GCAACTCCTT TGACATGCTG 
AGAAACCTCA TCGTCCCCAA ACAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025149554.1
CDS	16..2625
Translation	MAWDGGWGSRGANQRGLALSPDLLQRSLYKNPWEDLPRGQPGEGLDRLPPPPPASEHLSPETSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQTPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESCIERNPPEPDTMEDPQSPGSASTRGPGGASGGSEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSSGLEGLADDLRPERDERRVNVTGKAPNILLYVGSDSRDGQDRLQQVQSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLMRLSVLSSGCVYEEGPREPLGPGKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPVVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDAKQAPALTPLYLLTATEEIRDPLVQWLGKHVDPEGVIRSSKLSLKFISSYTSEIEITPSALPVVTDTESFSSENFDFEIYRQNLKTKKLGKVILFAEVTPTTMNLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTVTVLEKLIDVFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_025293769.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis holocarboxylase synthetase (HLCS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025293769.1

ORF Insert Sequence:

ATGGCTTGGG ATGGGGGATG GGGGTCCAGA GGAGCCAACC AAAGAGGTTT GGCACTTTCT 
CCTGACCTGC TTCAAAGAAG CCTCTATAAA AACCCTTGGG AAGATCTACC AAGAGGGCAA 
CCCGGAGAGG GCTTGGACCG TCTGCCTCCC CCACCTCCTG CTTCTGAGCA TCTCTCACCG 
GAAACATCCA TTGATGACTT AAACAAATGG GCCCTGTTCC TGGTTTCTCC TTTTATACTT 
GAAGCAGAGC ACATCGCCTT TGTGACAGAG AGCATTTGGG TGCAAGGTGA GAATTTACAG 
ACACCGGCAT CCTCTTCGGA AACTATTGTG AAGTGGTCTG ACTGCTGTTT GCCGTTAGCT 
TGCCGACCTG GGGATCCTTA TCAATTAATC GCCAAAGCAA GTGTGGATGA CTTCAGCAAG 
CTAGGGGTGG CATTCCTGGA AGATAGACTC CAGATGGCTA ATGGACTGAT ACCCCAGAAG 
ATTGTGTCTG TCCACTTGCA GGACTCTGCC CTAAAGGAAC TTAAGGGTCA GGCCTCAGAC 
CCGCCAGCCC AGCTCCTGCA GCCTGACCCT GAATCTTGTA TTGAGAGGAA CCCTCCTGAG 
CCCGACACTA TGGAGGACCC CCAGTCTCCG GGTTCTGCAT CCACACGGGG GCCAGGCGGG 
GCCTCTGGGG GCAGCGAGCC AGCCGTAGAC GGTGACAGAG GCGTGGGCTC TGTCGAGCAC 
TGTCCCCTCC ATCTGTCCAG CTGCCATGAG TGTTTGGAGC TTGAGAACAG CACCATTGAA 
TCTGTCAGGT TTGCCTCTGC AGAGAACATT CCAGATCTCC CGTACGACTA CAGCAGCGGT 
TTGGAGGGCC TCGCCGACGA CCTCCGCCCC GAGAGGGATG AGAGGAGGGT CAATGTCACG 
GGCAAGGCCC CCAACATCCT CCTTTACGTG GGCTCTGACT CTCGGGATGG CCAGGACAGG 
CTCCAGCAGG TCCAATCTGC GCTGGCCGAC TGTGTGGACA CGGACTGTTA TACTCTGTAC 
CACCTGCCTC CAGAAAGCGC TCTCAGGGAC CCGTGGCCGG ACAACTGTCT GCTGCTGGTC 
ATCGCCACCG GGGAGCCCGT CCCCGAGGAC CTGTCCCGGA GGTTCACGGC CTATCTTTCT 
CAGGGAGGGA AGGTGCTGGC CCTGGCGTCA TCCTTCACGC TGCCTGGCTT CCAGGTGACC 
AGCAAGGCCC CGCTGCAGGA CACGGTCCAG AGCTTGGTTT TCTGCAAGGT GGACGGAAGC 
CTGATGAGGC TCAGTGTCCT GAGCAGCGGC TGCGTTTACG AGGAGGGGCC CAGGGAGCCG 
CTCGGCCCCG GCAAGCTCCA CGGCCACCTG GACAGTGAGG ATGGGGACAG GCTTGTGGTG 
CAAGTGCCTT TTGGGACTCA CGGAGGAGAA GCTGTTCTTT GCCAGGTGCA CTTGGAACTA 
CCTCCCAGCT CTCCAGTAGT GCAAACACAA GAGGATTTTA ACCTGCTTAA ATCAAGCAAC 
TTTAGAAGAT ACGAAGTCCT TACGGAGATC CTAACGACGC TGGGCCTAAG CTGTGACGCA 
AAGCAAGCTC CTGCCTTAAC GCCTCTTTAC CTGCTGACGG CGACCGAGGA AATCCGGGAT 
CCTCTTGTGC AGTGGCTGGG GAAACATGTG GATCCTGAAG GAGTAATAAG ATCGAGCAAA 
CTCTCCCTGA AATTCATTTC ATCCTATACA TCTGAAATAG AAATCACCCC ATCCGCCCTA 
CCCGTGGTGA CTGACACAGA GTCCTTCTCA TCAGAAAACT TTGACTTCGA GATTTACCGA 
CAAAACCTGA AGACAAAGAA ACTTGGGAAA GTCATTCTGT TTGCCGAAGT GACACCCACT 
ACAATGAATC TTCTGGACGG GTTGATGTTT GAGATGCCGC AGGAAATGGG TTTAATAGCC 
ATCGCAGTCC GCCAGACGCA GGGCAAAGGG CGGGGAAAGA ATGCTTGGCT GAGCCCCTTG 
GGATGCGCCC TTTCCACCCT GCTGGTCTGC ATCCCGCTGA GATCCCCGCT GGGACAGAGG 
ATTCCGTTTG TCCAGCATCT GATGTCCCTG GCCGTCGTAG AGGCAGTAAG ATCCATCCCC 
GGGTACCAGG ACATCAATTT GCGAGTGAAG TGGCCCAATG ATATTTATTA CAGTGACCTC 
ATGAAGCTTG GTGGAGTTTT GGTTAACTCA ACACTTATGG GAGAAACATT CTATATACTT 
ATTGGCTGTG GATTTAATGT GACAAACAGC AACCCTACCA TATGCATCAA TGATCTCATC 
ACAGAATACA ATAAGGAACA CGGGGCAGAG CTACAGCCCC TGAGAGCCGA CTGTCTCATC 
GCCCGAACAG TGACCGTGCT GGAGAAGCTG ATTGACGTGT TTCAGGACCA GGGGCCCAAC 
GGTGTTCTTC CTCTCTACTA TAAATACTGG GTACACAGCG CTCAGCAAGT CCACCTGGGA 
AGCGCTGAGG GACCAAAGGT GTGGATAGTT GGCCTGGATG ATTCTGGGTT CCTTCTGGTC 
CACCAGGAAA ACGGCGATGT GGTCACGGTG CATCCTGACG GCAACTCCTT TGACATGCTG 
AGAAACCTCA TCGTCCCCAA ACAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_025293775.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025149560.1 Bubalus bubalis holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu43467
Clone ID Related Accession (Same CDS sequence)	XM_025293775.1
Accession Version	XM_025293775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	biotin--protein ligase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGG ATCCAGATGC AAGGTCCATT GATGACTTAA ACAAATGGGC CCTGTTCCTG 
GTTTCTCCTT TTATACTTGA AGCAGAGCAC ATCGCCTTTG TGACAGAGAG CATTTGGGTG 
CAAGGTGAGA ATTTACAGAC ACCGGCATCC TCTTCGGAAA CTATTGTGAA GTGGTCTGAC 
TGCTGTTTGC CGTTAGCTTG CCGACCTGGG GATCCTTATC AATTAATCGC CAAAGCAAGT 
GTGGATGACT TCAGCAAGCT AGGGGTGGCA TTCCTGGAAG ATAGACTCCA GATGGCTAAT 
GGACTGATAC CCCAGAAGAT TGTGTCTGTC CACTTGCAGG ACTCTGCCCT AAAGGAACTT 
AAGGGTCAGG CCTCAGACCC GCCAGCCCAG CTCCTGCAGC CTGACCCTGA ATCTTGTATT 
GAGAGGAACC CTCCTGAGCC CGACACTATG GAGGACCCCC AGTCTCCGGG TTCTGCATCC 
ACACGGGGGC CAGGCGGGGC CTCTGGGGGC AGCGAGCCAG CCGTAGACGG TGACAGAGGC 
GTGGGCTCTG TCGAGCACTG TCCCCTCCAT CTGTCCAGCT GCCATGAGTG TTTGGAGCTT 
GAGAACAGCA CCATTGAATC TGTCAGGTTT GCCTCTGCAG AGAACATTCC AGATCTCCCG 
TACGACTACA GCAGCGGTTT GGAGGGCCTC GCCGACGACC TCCGCCCCGA GAGGGATGAG 
AGGAGGGTCA ATGTCACGGG CAAGGCCCCC AACATCCTCC TTTACGTGGG CTCTGACTCT 
CGGGATGGCC AGGACAGGCT CCAGCAGGTC CAATCTGCGC TGGCCGACTG TGTGGACACG 
GACTGTTATA CTCTGTACCA CCTGCCTCCA GAAAGCGCTC TCAGGGACCC GTGGCCGGAC 
AACTGTCTGC TGCTGGTCAT CGCCACCGGG GAGCCCGTCC CCGAGGACCT GTCCCGGAGG 
TTCACGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGCCC TGGCGTCATC CTTCACGCTG 
CCTGGCTTCC AGGTGACCAG CAAGGCCCCG CTGCAGGACA CGGTCCAGAG CTTGGTTTTC 
TGCAAGGTGG ACGGAAGCCT GATGAGGCTC AGTGTCCTGA GCAGCGGCTG CGTTTACGAG 
GAGGGGCCCA GGGAGCCGCT CGGCCCCGGC AAGCTCCACG GCCACCTGGA CAGTGAGGAT 
GGGGACAGGC TTGTGGTGCA AGTGCCTTTT GGGACTCACG GAGGAGAAGC TGTTCTTTGC 
CAGGTGCACT TGGAACTACC TCCCAGCTCT CCAGTAGTGC AAACACAAGA GGATTTTAAC 
CTGCTTAAAT CAAGCAACTT TAGAAGATAC GAAGTCCTTA CGGAGATCCT AACGACGCTG 
GGCCTAAGCT GTGACGCAAA GCAAGCTCCT GCCTTAACGC CTCTTTACCT GCTGACGGCG 
ACCGAGGAAA TCCGGGATCC TCTTGTGCAG TGGCTGGGGA AACATGTGGA TCCTGAAGGA 
GTAATAAGAT CGAGCAAACT CTCCCTGAAA TTCATTTCAT CCTATACATC TGAAATAGAA 
ATCACCCCAT CCGCCCTACC CGTGGTGACT GACACAGAGT CCTTCTCATC AGAAAACTTT 
GACTTCGAGA TTTACCGACA AAACCTGAAG ACAAAGAAAC TTGGGAAAGT CATTCTGTTT 
GCCGAAGTGA CACCCACTAC AATGAATCTT CTGGACGGGT TGATGTTTGA GATGCCGCAG 
GAAATGGGTT TAATAGCCAT CGCAGTCCGC CAGACGCAGG GCAAAGGGCG GGGAAAGAAT 
GCTTGGCTGA GCCCCTTGGG ATGCGCCCTT TCCACCCTGC TGGTCTGCAT CCCGCTGAGA 
TCCCCGCTGG GACAGAGGAT TCCGTTTGTC CAGCATCTGA TGTCCCTGGC CGTCGTAGAG 
GCAGTAAGAT CCATCCCCGG GTACCAGGAC ATCAATTTGC GAGTGAAGTG GCCCAATGAT 
ATTTATTACA GTGACCTCAT GAAGCTTGGT GGAGTTTTGG TTAACTCAAC ACTTATGGGA 
GAAACATTCT ATATACTTAT TGGCTGTGGA TTTAATGTGA CAAACAGCAA CCCTACCATA 
TGCATCAATG ATCTCATCAC AGAATACAAT AAGGAACACG GGGCAGAGCT ACAGCCCCTG 
AGAGCCGACT GTCTCATCGC CCGAACAGTG ACCGTGCTGG AGAAGCTGAT TGACGTGTTT 
CAGGACCAGG GGCCCAACGG TGTTCTTCCT CTCTACTATA AATACTGGGT ACACAGCGCT 
CAGCAAGTCC ACCTGGGAAG CGCTGAGGGA CCAAAGGTGT GGATAGTTGG CCTGGATGAT 
TCTGGGTTCC TTCTGGTCCA CCAGGAAAAC GGCGATGTGG TCACGGTGCA TCCTGACGGC 
AACTCCTTTG ACATGCTGAG AAACCTCATC GTCCCCAAAC AGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025149560.1
CDS	160..2607
Translation	MTQDPDARSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQTPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESCIERNPPEPDTMEDPQSPGSASTRGPGGASGGSEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSSGLEGLADDLRPERDERRVNVTGKAPNILLYVGSDSRDGQDRLQQVQSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLMRLSVLSSGCVYEEGPREPLGPGKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPVVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDAKQAPALTPLYLLTATEEIRDPLVQWLGKHVDPEGVIRSSKLSLKFISSYTSEIEITPSALPVVTDTESFSSENFDFEIYRQNLKTKKLGKVILFAEVTPTTMNLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTVTVLEKLIDVFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_025293775.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis holocarboxylase synthetase (HLCS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025293775.1

ORF Insert Sequence:

ATGACCCAGG ATCCAGATGC AAGGTCCATT GATGACTTAA ACAAATGGGC CCTGTTCCTG 
GTTTCTCCTT TTATACTTGA AGCAGAGCAC ATCGCCTTTG TGACAGAGAG CATTTGGGTG 
CAAGGTGAGA ATTTACAGAC ACCGGCATCC TCTTCGGAAA CTATTGTGAA GTGGTCTGAC 
TGCTGTTTGC CGTTAGCTTG CCGACCTGGG GATCCTTATC AATTAATCGC CAAAGCAAGT 
GTGGATGACT TCAGCAAGCT AGGGGTGGCA TTCCTGGAAG ATAGACTCCA GATGGCTAAT 
GGACTGATAC CCCAGAAGAT TGTGTCTGTC CACTTGCAGG ACTCTGCCCT AAAGGAACTT 
AAGGGTCAGG CCTCAGACCC GCCAGCCCAG CTCCTGCAGC CTGACCCTGA ATCTTGTATT 
GAGAGGAACC CTCCTGAGCC CGACACTATG GAGGACCCCC AGTCTCCGGG TTCTGCATCC 
ACACGGGGGC CAGGCGGGGC CTCTGGGGGC AGCGAGCCAG CCGTAGACGG TGACAGAGGC 
GTGGGCTCTG TCGAGCACTG TCCCCTCCAT CTGTCCAGCT GCCATGAGTG TTTGGAGCTT 
GAGAACAGCA CCATTGAATC TGTCAGGTTT GCCTCTGCAG AGAACATTCC AGATCTCCCG 
TACGACTACA GCAGCGGTTT GGAGGGCCTC GCCGACGACC TCCGCCCCGA GAGGGATGAG 
AGGAGGGTCA ATGTCACGGG CAAGGCCCCC AACATCCTCC TTTACGTGGG CTCTGACTCT 
CGGGATGGCC AGGACAGGCT CCAGCAGGTC CAATCTGCGC TGGCCGACTG TGTGGACACG 
GACTGTTATA CTCTGTACCA CCTGCCTCCA GAAAGCGCTC TCAGGGACCC GTGGCCGGAC 
AACTGTCTGC TGCTGGTCAT CGCCACCGGG GAGCCCGTCC CCGAGGACCT GTCCCGGAGG 
TTCACGGCCT ATCTTTCTCA GGGAGGGAAG GTGCTGGCCC TGGCGTCATC CTTCACGCTG 
CCTGGCTTCC AGGTGACCAG CAAGGCCCCG CTGCAGGACA CGGTCCAGAG CTTGGTTTTC 
TGCAAGGTGG ACGGAAGCCT GATGAGGCTC AGTGTCCTGA GCAGCGGCTG CGTTTACGAG 
GAGGGGCCCA GGGAGCCGCT CGGCCCCGGC AAGCTCCACG GCCACCTGGA CAGTGAGGAT 
GGGGACAGGC TTGTGGTGCA AGTGCCTTTT GGGACTCACG GAGGAGAAGC TGTTCTTTGC 
CAGGTGCACT TGGAACTACC TCCCAGCTCT CCAGTAGTGC AAACACAAGA GGATTTTAAC 
CTGCTTAAAT CAAGCAACTT TAGAAGATAC GAAGTCCTTA CGGAGATCCT AACGACGCTG 
GGCCTAAGCT GTGACGCAAA GCAAGCTCCT GCCTTAACGC CTCTTTACCT GCTGACGGCG 
ACCGAGGAAA TCCGGGATCC TCTTGTGCAG TGGCTGGGGA AACATGTGGA TCCTGAAGGA 
GTAATAAGAT CGAGCAAACT CTCCCTGAAA TTCATTTCAT CCTATACATC TGAAATAGAA 
ATCACCCCAT CCGCCCTACC CGTGGTGACT GACACAGAGT CCTTCTCATC AGAAAACTTT 
GACTTCGAGA TTTACCGACA AAACCTGAAG ACAAAGAAAC TTGGGAAAGT CATTCTGTTT 
GCCGAAGTGA CACCCACTAC AATGAATCTT CTGGACGGGT TGATGTTTGA GATGCCGCAG 
GAAATGGGTT TAATAGCCAT CGCAGTCCGC CAGACGCAGG GCAAAGGGCG GGGAAAGAAT 
GCTTGGCTGA GCCCCTTGGG ATGCGCCCTT TCCACCCTGC TGGTCTGCAT CCCGCTGAGA 
TCCCCGCTGG GACAGAGGAT TCCGTTTGTC CAGCATCTGA TGTCCCTGGC CGTCGTAGAG 
GCAGTAAGAT CCATCCCCGG GTACCAGGAC ATCAATTTGC GAGTGAAGTG GCCCAATGAT 
ATTTATTACA GTGACCTCAT GAAGCTTGGT GGAGTTTTGG TTAACTCAAC ACTTATGGGA 
GAAACATTCT ATATACTTAT TGGCTGTGGA TTTAATGTGA CAAACAGCAA CCCTACCATA 
TGCATCAATG ATCTCATCAC AGAATACAAT AAGGAACACG GGGCAGAGCT ACAGCCCCTG 
AGAGCCGACT GTCTCATCGC CCGAACAGTG ACCGTGCTGG AGAAGCTGAT TGACGTGTTT 
CAGGACCAGG GGCCCAACGG TGTTCTTCCT CTCTACTATA AATACTGGGT ACACAGCGCT 
CAGCAAGTCC ACCTGGGAAG CGCTGAGGGA CCAAAGGTGT GGATAGTTGG CCTGGATGAT 
TCTGGGTTCC TTCTGGTCCA CCAGGAAAAC GGCGATGTGG TCACGGTGCA TCCTGACGGC 
AACTCCTTTG ACATGCTGAG AAACCTCATC GTCCCCAAAC AGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLCS ( XM_025293779.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025149564.1 Bubalus bubalis holocarboxylase synthetase (HLCS), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu43468
Clone ID Related Accession (Same CDS sequence)	XM_025293779.1
Accession Version	XM_025293779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	biotin--protein ligase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTCGA CATCCATTGA TGACTTAAAC AAATGGGCCC TGTTCCTGGT TTCTCCTTTT 
ATACTTGAAG CAGAGCACAT CGCCTTTGTG ACAGAGAGCA TTTGGGTGCA AGGTGAGAAT 
TTACAGACAC CGGCATCCTC TTCGGAAACT ATTGTGAAGT GGTCTGACTG CTGTTTGCCG 
TTAGCTTGCC GACCTGGGGA TCCTTATCAA TTAATCGCCA AAGCAAGTGT GGATGACTTC 
AGCAAGCTAG GGGTGGCATT CCTGGAAGAT AGACTCCAGA TGGCTAATGG ACTGATACCC 
CAGAAGATTG TGTCTGTCCA CTTGCAGGAC TCTGCCCTAA AGGAACTTAA GGGTCAGGCC 
TCAGACCCGC CAGCCCAGCT CCTGCAGCCT GACCCTGAAT CTTGTATTGA GAGGAACCCT 
CCTGAGCCCG ACACTATGGA GGACCCCCAG TCTCCGGGTT CTGCATCCAC ACGGGGGCCA 
GGCGGGGCCT CTGGGGGCAG CGAGCCAGCC GTAGACGGTG ACAGAGGCGT GGGCTCTGTC 
GAGCACTGTC CCCTCCATCT GTCCAGCTGC CATGAGTGTT TGGAGCTTGA GAACAGCACC 
ATTGAATCTG TCAGGTTTGC CTCTGCAGAG AACATTCCAG ATCTCCCGTA CGACTACAGC 
AGCGGTTTGG AGGGCCTCGC CGACGACCTC CGCCCCGAGA GGGATGAGAG GAGGGTCAAT 
GTCACGGGCA AGGCCCCCAA CATCCTCCTT TACGTGGGCT CTGACTCTCG GGATGGCCAG 
GACAGGCTCC AGCAGGTCCA ATCTGCGCTG GCCGACTGTG TGGACACGGA CTGTTATACT 
CTGTACCACC TGCCTCCAGA AAGCGCTCTC AGGGACCCGT GGCCGGACAA CTGTCTGCTG 
CTGGTCATCG CCACCGGGGA GCCCGTCCCC GAGGACCTGT CCCGGAGGTT CACGGCCTAT 
CTTTCTCAGG GAGGGAAGGT GCTGGCCCTG GCGTCATCCT TCACGCTGCC TGGCTTCCAG 
GTGACCAGCA AGGCCCCGCT GCAGGACACG GTCCAGAGCT TGGTTTTCTG CAAGGTGGAC 
GGAAGCCTGA TGAGGCTCAG TGTCCTGAGC AGCGGCTGCG TTTACGAGGA GGGGCCCAGG 
GAGCCGCTCG GCCCCGGCAA GCTCCACGGC CACCTGGACA GTGAGGATGG GGACAGGCTT 
GTGGTGCAAG TGCCTTTTGG GACTCACGGA GGAGAAGCTG TTCTTTGCCA GGTGCACTTG 
GAACTACCTC CCAGCTCTCC AGTAGTGCAA ACACAAGAGG ATTTTAACCT GCTTAAATCA 
AGCAACTTTA GAAGATACGA AGTCCTTACG GAGATCCTAA CGACGCTGGG CCTAAGCTGT 
GACGCAAAGC AAGCTCCTGC CTTAACGCCT CTTTACCTGC TGACGGCGAC CGAGGAAATC 
CGGGATCCTC TTGTGCAGTG GCTGGGGAAA CATGTGGATC CTGAAGGAGT AATAAGATCG 
AGCAAACTCT CCCTGAAATT CATTTCATCC TATACATCTG AAATAGAAAT CACCCCATCC 
GCCCTACCCG TGGTGACTGA CACAGAGTCC TTCTCATCAG AAAACTTTGA CTTCGAGATT 
TACCGACAAA ACCTGAAGAC AAAGAAACTT GGGAAAGTCA TTCTGTTTGC CGAAGTGACA 
CCCACTACAA TGAATCTTCT GGACGGGTTG ATGTTTGAGA TGCCGCAGGA AATGGGTTTA 
ATAGCCATCG CAGTCCGCCA GACGCAGGGC AAAGGGCGGG GAAAGAATGC TTGGCTGAGC 
CCCTTGGGAT GCGCCCTTTC CACCCTGCTG GTCTGCATCC CGCTGAGATC CCCGCTGGGA 
CAGAGGATTC CGTTTGTCCA GCATCTGATG TCCCTGGCCG TCGTAGAGGC AGTAAGATCC 
ATCCCCGGGT ACCAGGACAT CAATTTGCGA GTGAAGTGGC CCAATGATAT TTATTACAGT 
GACCTCATGA AGCTTGGTGG AGTTTTGGTT AACTCAACAC TTATGGGAGA AACATTCTAT 
ATACTTATTG GCTGTGGATT TAATGTGACA AACAGCAACC CTACCATATG CATCAATGAT 
CTCATCACAG AATACAATAA GGAACACGGG GCAGAGCTAC AGCCCCTGAG AGCCGACTGT 
CTCATCGCCC GAACAGTGAC CGTGCTGGAG AAGCTGATTG ACGTGTTTCA GGACCAGGGG 
CCCAACGGTG TTCTTCCTCT CTACTATAAA TACTGGGTAC ACAGCGCTCA GCAAGTCCAC 
CTGGGAAGCG CTGAGGGACC AAAGGTGTGG ATAGTTGGCC TGGATGATTC TGGGTTCCTT 
CTGGTCCACC AGGAAAACGG CGATGTGGTC ACGGTGCATC CTGACGGCAA CTCCTTTGAC 
ATGCTGAGAA ACCTCATCGT CCCCAAACAG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025149564.1
CDS	16..2451
Translation	MCSTSIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQTPASSSETIVKWSDCCLPLACRPGDPYQLIAKASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQDSALKELKGQASDPPAQLLQPDPESCIERNPPEPDTMEDPQSPGSASTRGPGGASGGSEPAVDGDRGVGSVEHCPLHLSSCHECLELENSTIESVRFASAENIPDLPYDYSSGLEGLADDLRPERDERRVNVTGKAPNILLYVGSDSRDGQDRLQQVQSALADCVDTDCYTLYHLPPESALRDPWPDNCLLLVIATGEPVPEDLSRRFTAYLSQGGKVLALASSFTLPGFQVTSKAPLQDTVQSLVFCKVDGSLMRLSVLSSGCVYEEGPREPLGPGKLHGHLDSEDGDRLVVQVPFGTHGGEAVLCQVHLELPPSSPVVQTQEDFNLLKSSNFRRYEVLTEILTTLGLSCDAKQAPALTPLYLLTATEEIRDPLVQWLGKHVDPEGVIRSSKLSLKFISSYTSEIEITPSALPVVTDTESFSSENFDFEIYRQNLKTKKLGKVILFAEVTPTTMNLLDGLMFEMPQEMGLIAIAVRQTQGKGRGKNAWLSPLGCALSTLLVCIPLRSPLGQRIPFVQHLMSLAVVEAVRSIPGYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKEHGAELQPLRADCLIARTVTVLEKLIDVFQDQGPNGVLPLYYKYWVHSAQQVHLGSAEGPKVWIVGLDDSGFLLVHQENGDVVTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_025293779.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis holocarboxylase synthetase (HLCS), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025293779.1

ORF Insert Sequence:

ATGTGCTCGA CATCCATTGA TGACTTAAAC AAATGGGCCC TGTTCCTGGT TTCTCCTTTT 
ATACTTGAAG CAGAGCACAT CGCCTTTGTG ACAGAGAGCA TTTGGGTGCA AGGTGAGAAT 
TTACAGACAC CGGCATCCTC TTCGGAAACT ATTGTGAAGT GGTCTGACTG CTGTTTGCCG 
TTAGCTTGCC GACCTGGGGA TCCTTATCAA TTAATCGCCA AAGCAAGTGT GGATGACTTC 
AGCAAGCTAG GGGTGGCATT CCTGGAAGAT AGACTCCAGA TGGCTAATGG ACTGATACCC 
CAGAAGATTG TGTCTGTCCA CTTGCAGGAC TCTGCCCTAA AGGAACTTAA GGGTCAGGCC 
TCAGACCCGC CAGCCCAGCT CCTGCAGCCT GACCCTGAAT CTTGTATTGA GAGGAACCCT 
CCTGAGCCCG ACACTATGGA GGACCCCCAG TCTCCGGGTT CTGCATCCAC ACGGGGGCCA 
GGCGGGGCCT CTGGGGGCAG CGAGCCAGCC GTAGACGGTG ACAGAGGCGT GGGCTCTGTC 
GAGCACTGTC CCCTCCATCT GTCCAGCTGC CATGAGTGTT TGGAGCTTGA GAACAGCACC 
ATTGAATCTG TCAGGTTTGC CTCTGCAGAG AACATTCCAG ATCTCCCGTA CGACTACAGC 
AGCGGTTTGG AGGGCCTCGC CGACGACCTC CGCCCCGAGA GGGATGAGAG GAGGGTCAAT 
GTCACGGGCA AGGCCCCCAA CATCCTCCTT TACGTGGGCT CTGACTCTCG GGATGGCCAG 
GACAGGCTCC AGCAGGTCCA ATCTGCGCTG GCCGACTGTG TGGACACGGA CTGTTATACT 
CTGTACCACC TGCCTCCAGA AAGCGCTCTC AGGGACCCGT GGCCGGACAA CTGTCTGCTG 
CTGGTCATCG CCACCGGGGA GCCCGTCCCC GAGGACCTGT CCCGGAGGTT CACGGCCTAT 
CTTTCTCAGG GAGGGAAGGT GCTGGCCCTG GCGTCATCCT TCACGCTGCC TGGCTTCCAG 
GTGACCAGCA AGGCCCCGCT GCAGGACACG GTCCAGAGCT TGGTTTTCTG CAAGGTGGAC 
GGAAGCCTGA TGAGGCTCAG TGTCCTGAGC AGCGGCTGCG TTTACGAGGA GGGGCCCAGG 
GAGCCGCTCG GCCCCGGCAA GCTCCACGGC CACCTGGACA GTGAGGATGG GGACAGGCTT 
GTGGTGCAAG TGCCTTTTGG GACTCACGGA GGAGAAGCTG TTCTTTGCCA GGTGCACTTG 
GAACTACCTC CCAGCTCTCC AGTAGTGCAA ACACAAGAGG ATTTTAACCT GCTTAAATCA 
AGCAACTTTA GAAGATACGA AGTCCTTACG GAGATCCTAA CGACGCTGGG CCTAAGCTGT 
GACGCAAAGC AAGCTCCTGC CTTAACGCCT CTTTACCTGC TGACGGCGAC CGAGGAAATC 
CGGGATCCTC TTGTGCAGTG GCTGGGGAAA CATGTGGATC CTGAAGGAGT AATAAGATCG 
AGCAAACTCT CCCTGAAATT CATTTCATCC TATACATCTG AAATAGAAAT CACCCCATCC 
GCCCTACCCG TGGTGACTGA CACAGAGTCC TTCTCATCAG AAAACTTTGA CTTCGAGATT 
TACCGACAAA ACCTGAAGAC AAAGAAACTT GGGAAAGTCA TTCTGTTTGC CGAAGTGACA 
CCCACTACAA TGAATCTTCT GGACGGGTTG ATGTTTGAGA TGCCGCAGGA AATGGGTTTA 
ATAGCCATCG CAGTCCGCCA GACGCAGGGC AAAGGGCGGG GAAAGAATGC TTGGCTGAGC 
CCCTTGGGAT GCGCCCTTTC CACCCTGCTG GTCTGCATCC CGCTGAGATC CCCGCTGGGA 
CAGAGGATTC CGTTTGTCCA GCATCTGATG TCCCTGGCCG TCGTAGAGGC AGTAAGATCC 
ATCCCCGGGT ACCAGGACAT CAATTTGCGA GTGAAGTGGC CCAATGATAT TTATTACAGT 
GACCTCATGA AGCTTGGTGG AGTTTTGGTT AACTCAACAC TTATGGGAGA AACATTCTAT 
ATACTTATTG GCTGTGGATT TAATGTGACA AACAGCAACC CTACCATATG CATCAATGAT 
CTCATCACAG AATACAATAA GGAACACGGG GCAGAGCTAC AGCCCCTGAG AGCCGACTGT 
CTCATCGCCC GAACAGTGAC CGTGCTGGAG AAGCTGATTG ACGTGTTTCA GGACCAGGGG 
CCCAACGGTG TTCTTCCTCT CTACTATAAA TACTGGGTAC ACAGCGCTCA GCAAGTCCAC 
CTGGGAAGCG CTGAGGGACC AAAGGTGTGG ATAGTTGGCC TGGATGATTC TGGGTTCCTT 
CTGGTCCACC AGGAAAACGG CGATGTGGTC ACGGTGCATC CTGACGGCAA CTCCTTTGAC 
ATGCTGAGAA ACCTCATCGT CCCCAAACAG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HLCS cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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