THOC2 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006064992.1	XP_006065054.1	THO complex subunit 2-like isoform X2
XM_006065001.1	XP_006065063.1	THO complex subunit 2-like isoform X11
XM_025276457.1	XP_025132242.1	THO complex subunit 2 isoform X1
XM_025276461.1	XP_025132246.1	THO complex subunit 2 isoform X3
XM_006064994.2	XP_006065056.2	THO complex subunit 2 isoform X6
XM_006064996.2	XP_006065058.2	THO complex subunit 2 isoform X7
XM_006064995.1	XP_006065057.1	THO complex subunit 2-like isoform X5
XM_006065000.1	XP_006065062.1	THO complex subunit 2-like isoform X10
XM_025276459.1	XP_025132244.1	THO complex subunit 2 isoform X2
XM_006064997.1	XP_006065059.1	THO complex subunit 2-like isoform X7
XM_006064999.1	XP_006065061.1	THO complex subunit 2-like isoform X9
XM_025276458.1	XP_025132243.1	THO complex subunit 2 isoform X1
XM_006064993.1	XP_006065055.1	THO complex subunit 2-like isoform X3
XM_006064994.1	XP_006065056.1	THO complex subunit 2-like isoform X4
XM_006064996.1	XP_006065058.1	THO complex subunit 2-like isoform X6
XM_006064998.1	XP_006065060.1	THO complex subunit 2-like isoform X8
XM_025276460.1	XP_025132245.1	THO complex subunit 2 isoform X3
XM_025276462.1	XP_025132247.1	THO complex subunit 2 isoform X4
XM_025276463.1	XP_025132248.1	THO complex subunit 2 isoform X5
XM_025276464.1	XP_025132249.1	THO complex subunit 2 isoform X9
XM_006064991.1	XP_006065053.1	THO complex subunit 2-like isoform X1
XM_006064995.2	XP_006065057.2	THO complex subunit 2 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu20613	XM_006064999.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu20614	XM_006065000.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu20615	XM_006065001.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu73761	XM_025276464.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu20606	XM_006064991.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu20607	XM_006064992.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu20608	XM_006064993.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu20609	XM_006064994.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu20610	XM_006064995.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu20611	XM_006064996.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu20611	XM_006064997.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu20612	XM_006064998.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu73753	XM_025276458.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu73753	XM_025276457.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu73754	XM_025276459.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu73755	XM_025276461.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu73755	XM_025276460.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu73756	XM_025276462.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu73757	XM_025276463.1 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu73758	XM_006064994.2 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu73759	XM_006064996.2 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu73760	XM_006064995.2 Latest version!	Bubalus bubalis THO complex 2 (THOC2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

THOC2 ( XM_006064999.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065061.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBu20613
Clone ID Related Accession (Same CDS sequence)	XM_006064999.1
Accession Version	XM_006064999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2-like isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785155.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAT GAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGTTAC AGCCATACTT CCCAAAGTTC CTCTGGGTTC TGAGAACTAT 
GCCAGCTCAC CTGTCATCTC CATTCATTTT CTACAGGACA GTCTCATCGA ACTCAAGGAG 
TCTTCAGCAA AGATTAACCC AGAAGACCAA AAGATATATC TGATGCATTC ACATAACTCT 
TCTAAAAACC GAGCCCATCT CAACCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065061.1
CDS	39..4508
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKAREMKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKVTAILPKVPLGSENYASSPVISIHFLQDSLIELKESSAKINPEDQKIYLMHSHNSSKNRAHLNP

Target ORF information:

RefSeq Version	XM_006064999.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064999.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAT GAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGTTAC AGCCATACTT CCCAAAGTTC CTCTGGGTTC TGAGAACTAT 
GCCAGCTCAC CTGTCATCTC CATTCATTTT CTACAGGACA GTCTCATCGA ACTCAAGGAG 
TCTTCAGCAA AGATTAACCC AGAAGACCAA AAGATATATC TGATGCATTC ACATAACTCT 
TCTAAAAACC GAGCCCATCT CAACCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_006065000.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065062.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBu20614
Clone ID Related Accession (Same CDS sequence)	XM_006065000.1
Accession Version	XM_006065000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785155.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAT GAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGACAG TCTCATCGAA CTCAAGGAGT CTTCAGCAAA GATTAACCCA 
GAAGACCAAA AGATATATCT GATGCATTCA CATAACTCTT CTAAAAACCG AGCCCATCTC 
AACCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065062.1
CDS	39..4427
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKAREMKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKDSLIELKESSAKINPEDQKIYLMHSHNSSKNRAHLNP

Target ORF information:

RefSeq Version	XM_006065000.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006065000.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAT GAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGACAG TCTCATCGAA CTCAAGGAGT CTTCAGCAAA GATTAACCCA 
GAAGACCAAA AGATATATCT GATGCATTCA CATAACTCTT CTAAAAACCG AGCCCATCTC 
AACCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_006065001.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065063.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBu20615
Clone ID Related Accession (Same CDS sequence)	XM_006065001.1
Accession Version	XM_006065001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2-like isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785155.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAC ACAAACGAGA AATTGTAGAA GCTAAGCAGA TCGTTAGAAA ACTTACAATG 
GTTGTATTGT CTTCTGATAA AATTGACGAG CGAGAGAAAG AAAAGGAAAA AGAAGAGGAG 
AAAGTGGAGA AGCCACCTGA CAACCAAAAA CTTGGTTTGT TGGAAGCTTT GTTAAAGATT 
GGTGATTGGC AGCATGCGCA GAGCATTATG GATCAGATGC CTCCATACTA TGCAGCTTCA 
CATAAACTAA TAGCCCTTGC TATTTGCAAG CTGATTCATA TAACTATTGA GCCTCTCTAT 
CGAAGAGTTG GCGTTCCTAA AGGTGCTAAA GGCTCACCTG TTAGTGCTTT GCAAAATAAG 
AGAGCACCAA AACAAGCAGA GAGCTTTGAA GATTTGAGGA GAGACGTCTT CAATATGTTC 
TGTTACCTTG GTCCCCACCT TTCTCATGAT CCCATTTTAT TCGCAAAAGT GGTGCGGATA 
GGCAAGTCAT TTATGAAGGA GTTTCAGTCT GATGGAAGCA AACAAGAAGA TAAAGAAAAA 
ACGGAAGTTA TCCTTAGCTG TTTGCTTAGC ATTACTGACC AGGTATTACT TCCATCTCTT 
TCTTTGATGG ACTGCAATGC TTGTATGTCT GAGGAACTAT GGGGAATGTT TAAAACATTT 
CCATATCAGC ATAGATATCG TCTGTATGGC CAGTGGAAGA ATGAGACTTA CAACAGTCAT 
CCACTTTTAG TAAAAGTTAA AGCTCAAACA ATAGACAGAG CCAAATATAT CATGAAGCGT 
CTAACCAAGG AAAATGTGAA GCCTTCTGGA AGACAAATTG GGAAGTTGAG CCACAGCAAT 
CCAACCATTT TGTTTGATTA TATCTTGTCA CAAATACAGA AGTATGATAA CTTGATAACA 
CCTGTAGTAG ATTCATTGAA ATACCTCACT TCGTTGAATT ATGACGTCTT GGCCTATTGT 
ATCATTGAAG CTTTAGCTAA TCCAGAAAAG GAGAAAATGA AACATGATGA CACAACCATC 
TCAAGTTGGC TTCAGAGTCT GGCTAGTTTC TGTGGTGCAG TTTTTCGTAA ATATCCAATT 
GATCTTGCTG GTCTTCTTCA GTATGTGGCT AATCAGCTAA AGGCAGGCAA AAGTTTTGAC 
CTGCTTATAC TGAAAGAAGT GGTACAAAAA ATGGCAGGAA TAGAAATTAC AGAAGAAATG 
ACAATGGAGC AACTAGAAGC CATGACTGGT GGAGAGCAAC TAAAAGCTGA GGGTGGTTAT 
TTTGGCCAGA TAAGAAACAC GAAAAAATCC TCCCAGAGAT TAAAGGATGC ACTATTAGAC 
CATGATCTTG CTCTTCCTCT CTGCTTGCTT ATGGCTCAGC AGAGGAATGG GGTAATCTTT 
CAGGAAGGTG GAGAGAAACA TTTGAAACTT GTGGGAAAAC TCTATGATCA GTGTCATGAT 
ACCCTGGTAC AGTTTGGTGG GTTTTTAGCA TCTAATCTAA GCACAGAAGA TTATATAAAG 
CGAGTGCCTT CAATTGATGT GCTCTGTAAT GAATTTCACA CACCTCATGA TGCAGCATTT 
TTCCTGTCTA GGCCAATGTA TGCACACCAT ATTTCGTCAA AGTATGACGA ACTTAAAAAA 
TCAGAGAAGG GAAGTAAACA GCAGCATAAA GTTCATAAGT ACATCACATC ATGTGAAATG 
GTGATGGCTC CTGTTCATGA AGCAGTGGTT TCCTTACACG TTTCCAAAGT CTGGGATGAC 
ATCAGTCCTC AATTCTATGC CACATTCTGG TCATTGACAA TGTATGACCT TGCAGTTCCA 
CATACCAGCT ATGAACGGGA AGTCAATAAA CTTAAAATCC AGATGAAAGC AATTGATGAC 
AATCAGGAAA TGCCTCCAAA TAAAAAGAAA AAAGAGAAGG AGCGATGTAC TGCCCTTCAG 
GACAAGCTTC TCGAAGAAGA AAAGAAACAG ATGGAACATG TACAGCGAGT TCTGCAGAGA 
CTGAAACTGG AAAAGGACAA CTGGCTTTTA GCAAAATCTA CCAAAAATGA GACCATCACA 
AAATTTCTAC AGCTGTGTAT ATTTCCTCGA TGTATTTTTT CAGCAATTGA TGCTGTTTAC 
TGTGCTCGTT TTGTTGAATT GGTACATCAA CAGAAAACTC CAAATTTTTC CACACTTCTT 
TGCTATGATC GAGTTTTCTC TGATATAATT TACACAGTTG CAAGCTGTAC TGAAAATGAA 
GCTAGTCGAT ATGGGAGATT CCTTTGCTGC ATGTTAGAGA CTGTGACCAG GTGGCACAGT 
GATAGAGCCA CATATGAAAA GGAATGTGGA AATTATCCAG GATTCCTTAC TATATTACGA 
GCAACTGGAT TTGATGGTGG AAATAAAGCT GATCAATTAG ACTATGAAAA TTTTCGACAT 
GTTGTACACA AATGGCATTA CAAACTAACC AAGGCCTCGG TACATTGCCT TGAAACAGGC 
GAATATACTC ACATCAGGAA TATCTTGATT GTGCTAACAA AAATACTTCC TTGGTACCCA 
AAAGTTTTGA ATCTGGGTCA GGCATTGGAA AGAAGAGTGC ATAAAATCTG CCAAGAGGAA 
AAAGAGAAGA GGCCAGATCT ATATGCATTG GCTATGGGCT ACTCTGGGCA GTTGAAAAGT 
AGAAAGTCAT ACATGATACC TGAAAATGAG TTTCATCACA AAGACCCCCC TCCGAGGAAT 
GCAGTTGCCA GTGTACAAAA TGGGCCTGGT GGTGGGCCTT CTTCATCGTC GATCGGAAGT 
GTATCTAAGT CAGACGAAAG CAGTACCGAG GACACTGATA AATCAAGGGA GAGATCTCAG 
TGTGGTGTGA AAGCTGTTAA TAAAGCTTCT AGTGCCACAC CAAAAGGGAA TTCAAGTAAT 
GGAAATAGTG GCTCTAACAG CAGCAAAACT GTTAAAGAAA ATGACAAAGA AAAAGGAAAA 
GAGAAAGAAA AAGAGAAAAA AGAAAAGACT CCAGCTACTA CTCCAGAGGC CCGAGTACTT 
GGTAAAGATG GTAAAGAAAA ACCAAAGGAA GAACGGCCAA ATAAAGATGA AAAAGCAAGA 
GAGATGAAGG AAAGAACACC TAAATCTGAC AAAGAGAAAG AAAAATTCAA GAAGGAAGAA 
AAAGCTAAAG ATGAGAAATT CAAGACCACT GTCCCGAATG TAGAATCAAA GTCAACTCAA 
GAAAAGGAAA GAGAGAAGGA GCCGTCCAGA GAACGAGATA TAGCAAAGGA AATGAAATCA 
AAGGAAAATG TTAAAGGAGG AGAGAAAACA CCAGTTTCTG GGTCCTTGAA ATCACCTGTT 
CCCCGATCAG ATATTACAGA GCCTGAAAGG GAACAGAAAC GTCGCAAAAT TGATACCCAT 
CCTTCTCCAT CACATTCCTC AACAGTAAAG GTTACAGCCA TACTTCCCAA AGTTCCTCTG 
GGTTCTGAGA ACTATGCCAG CTCACCTGTC ATCTCCATTC ATTTTCTACA GGACAGTCTC 
ATCGAACTCA AGGAGTCTTC AGCAAAGCTC TACATTAATC ATACTCCTCC ACCACTGTCC 
AAGAGTAAGG AGAGAGAAAT GGACAAGAAA GATTTGGACA AGTCAAGGGA AAGATCCAGA 
GAAAGAGAAA AAAAAGATGA AAAGGACAGG AAAGAGCGGA AAAGGGATCA CTCAAACAAT 
GACCGAGAAG TGCCACCGGA CTTAACCAAG AGGCGGAAAG AGGAAAATGG AACAATGGGG 
GTTTCAAAGC ACAAAAGTGA AAGTCCATGT GAGTCTCCTT ATCCAAATGA GAAAGACAAG 
GAAAAAAATA AGTCAAAATC TTCAGGCAAA GAAAAAGGCG GCGACTCATT TAAATCTGAA 
AAGATGGATA AAATCTCCTC TGGTGGCAAA AAGGAGTCCA GGCATGATAA AGAAAAGATA 
GAAAAGAAAG AGAAACGGGA CAGTTCAGGA GGAAAGGAAG AGAAGAAACA TCATAAGTCC 
TCGGACAAGC ACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065063.1
CDS	333..4310
Translation	MDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKAREMKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKVTAILPKVPLGSENYASSPVISIHFLQDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKHHKSSDKHR

Target ORF information:

RefSeq Version	XM_006065001.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006065001.1

ORF Insert Sequence:

ATGGATGAAC ACAAACGAGA AATTGTAGAA GCTAAGCAGA TCGTTAGAAA ACTTACAATG 
GTTGTATTGT CTTCTGATAA AATTGACGAG CGAGAGAAAG AAAAGGAAAA AGAAGAGGAG 
AAAGTGGAGA AGCCACCTGA CAACCAAAAA CTTGGTTTGT TGGAAGCTTT GTTAAAGATT 
GGTGATTGGC AGCATGCGCA GAGCATTATG GATCAGATGC CTCCATACTA TGCAGCTTCA 
CATAAACTAA TAGCCCTTGC TATTTGCAAG CTGATTCATA TAACTATTGA GCCTCTCTAT 
CGAAGAGTTG GCGTTCCTAA AGGTGCTAAA GGCTCACCTG TTAGTGCTTT GCAAAATAAG 
AGAGCACCAA AACAAGCAGA GAGCTTTGAA GATTTGAGGA GAGACGTCTT CAATATGTTC 
TGTTACCTTG GTCCCCACCT TTCTCATGAT CCCATTTTAT TCGCAAAAGT GGTGCGGATA 
GGCAAGTCAT TTATGAAGGA GTTTCAGTCT GATGGAAGCA AACAAGAAGA TAAAGAAAAA 
ACGGAAGTTA TCCTTAGCTG TTTGCTTAGC ATTACTGACC AGGTATTACT TCCATCTCTT 
TCTTTGATGG ACTGCAATGC TTGTATGTCT GAGGAACTAT GGGGAATGTT TAAAACATTT 
CCATATCAGC ATAGATATCG TCTGTATGGC CAGTGGAAGA ATGAGACTTA CAACAGTCAT 
CCACTTTTAG TAAAAGTTAA AGCTCAAACA ATAGACAGAG CCAAATATAT CATGAAGCGT 
CTAACCAAGG AAAATGTGAA GCCTTCTGGA AGACAAATTG GGAAGTTGAG CCACAGCAAT 
CCAACCATTT TGTTTGATTA TATCTTGTCA CAAATACAGA AGTATGATAA CTTGATAACA 
CCTGTAGTAG ATTCATTGAA ATACCTCACT TCGTTGAATT ATGACGTCTT GGCCTATTGT 
ATCATTGAAG CTTTAGCTAA TCCAGAAAAG GAGAAAATGA AACATGATGA CACAACCATC 
TCAAGTTGGC TTCAGAGTCT GGCTAGTTTC TGTGGTGCAG TTTTTCGTAA ATATCCAATT 
GATCTTGCTG GTCTTCTTCA GTATGTGGCT AATCAGCTAA AGGCAGGCAA AAGTTTTGAC 
CTGCTTATAC TGAAAGAAGT GGTACAAAAA ATGGCAGGAA TAGAAATTAC AGAAGAAATG 
ACAATGGAGC AACTAGAAGC CATGACTGGT GGAGAGCAAC TAAAAGCTGA GGGTGGTTAT 
TTTGGCCAGA TAAGAAACAC GAAAAAATCC TCCCAGAGAT TAAAGGATGC ACTATTAGAC 
CATGATCTTG CTCTTCCTCT CTGCTTGCTT ATGGCTCAGC AGAGGAATGG GGTAATCTTT 
CAGGAAGGTG GAGAGAAACA TTTGAAACTT GTGGGAAAAC TCTATGATCA GTGTCATGAT 
ACCCTGGTAC AGTTTGGTGG GTTTTTAGCA TCTAATCTAA GCACAGAAGA TTATATAAAG 
CGAGTGCCTT CAATTGATGT GCTCTGTAAT GAATTTCACA CACCTCATGA TGCAGCATTT 
TTCCTGTCTA GGCCAATGTA TGCACACCAT ATTTCGTCAA AGTATGACGA ACTTAAAAAA 
TCAGAGAAGG GAAGTAAACA GCAGCATAAA GTTCATAAGT ACATCACATC ATGTGAAATG 
GTGATGGCTC CTGTTCATGA AGCAGTGGTT TCCTTACACG TTTCCAAAGT CTGGGATGAC 
ATCAGTCCTC AATTCTATGC CACATTCTGG TCATTGACAA TGTATGACCT TGCAGTTCCA 
CATACCAGCT ATGAACGGGA AGTCAATAAA CTTAAAATCC AGATGAAAGC AATTGATGAC 
AATCAGGAAA TGCCTCCAAA TAAAAAGAAA AAAGAGAAGG AGCGATGTAC TGCCCTTCAG 
GACAAGCTTC TCGAAGAAGA AAAGAAACAG ATGGAACATG TACAGCGAGT TCTGCAGAGA 
CTGAAACTGG AAAAGGACAA CTGGCTTTTA GCAAAATCTA CCAAAAATGA GACCATCACA 
AAATTTCTAC AGCTGTGTAT ATTTCCTCGA TGTATTTTTT CAGCAATTGA TGCTGTTTAC 
TGTGCTCGTT TTGTTGAATT GGTACATCAA CAGAAAACTC CAAATTTTTC CACACTTCTT 
TGCTATGATC GAGTTTTCTC TGATATAATT TACACAGTTG CAAGCTGTAC TGAAAATGAA 
GCTAGTCGAT ATGGGAGATT CCTTTGCTGC ATGTTAGAGA CTGTGACCAG GTGGCACAGT 
GATAGAGCCA CATATGAAAA GGAATGTGGA AATTATCCAG GATTCCTTAC TATATTACGA 
GCAACTGGAT TTGATGGTGG AAATAAAGCT GATCAATTAG ACTATGAAAA TTTTCGACAT 
GTTGTACACA AATGGCATTA CAAACTAACC AAGGCCTCGG TACATTGCCT TGAAACAGGC 
GAATATACTC ACATCAGGAA TATCTTGATT GTGCTAACAA AAATACTTCC TTGGTACCCA 
AAAGTTTTGA ATCTGGGTCA GGCATTGGAA AGAAGAGTGC ATAAAATCTG CCAAGAGGAA 
AAAGAGAAGA GGCCAGATCT ATATGCATTG GCTATGGGCT ACTCTGGGCA GTTGAAAAGT 
AGAAAGTCAT ACATGATACC TGAAAATGAG TTTCATCACA AAGACCCCCC TCCGAGGAAT 
GCAGTTGCCA GTGTACAAAA TGGGCCTGGT GGTGGGCCTT CTTCATCGTC GATCGGAAGT 
GTATCTAAGT CAGACGAAAG CAGTACCGAG GACACTGATA AATCAAGGGA GAGATCTCAG 
TGTGGTGTGA AAGCTGTTAA TAAAGCTTCT AGTGCCACAC CAAAAGGGAA TTCAAGTAAT 
GGAAATAGTG GCTCTAACAG CAGCAAAACT GTTAAAGAAA ATGACAAAGA AAAAGGAAAA 
GAGAAAGAAA AAGAGAAAAA AGAAAAGACT CCAGCTACTA CTCCAGAGGC CCGAGTACTT 
GGTAAAGATG GTAAAGAAAA ACCAAAGGAA GAACGGCCAA ATAAAGATGA AAAAGCAAGA 
GAGATGAAGG AAAGAACACC TAAATCTGAC AAAGAGAAAG AAAAATTCAA GAAGGAAGAA 
AAAGCTAAAG ATGAGAAATT CAAGACCACT GTCCCGAATG TAGAATCAAA GTCAACTCAA 
GAAAAGGAAA GAGAGAAGGA GCCGTCCAGA GAACGAGATA TAGCAAAGGA AATGAAATCA 
AAGGAAAATG TTAAAGGAGG AGAGAAAACA CCAGTTTCTG GGTCCTTGAA ATCACCTGTT 
CCCCGATCAG ATATTACAGA GCCTGAAAGG GAACAGAAAC GTCGCAAAAT TGATACCCAT 
CCTTCTCCAT CACATTCCTC AACAGTAAAG GTTACAGCCA TACTTCCCAA AGTTCCTCTG 
GGTTCTGAGA ACTATGCCAG CTCACCTGTC ATCTCCATTC ATTTTCTACA GGACAGTCTC 
ATCGAACTCA AGGAGTCTTC AGCAAAGCTC TACATTAATC ATACTCCTCC ACCACTGTCC 
AAGAGTAAGG AGAGAGAAAT GGACAAGAAA GATTTGGACA AGTCAAGGGA AAGATCCAGA 
GAAAGAGAAA AAAAAGATGA AAAGGACAGG AAAGAGCGGA AAAGGGATCA CTCAAACAAT 
GACCGAGAAG TGCCACCGGA CTTAACCAAG AGGCGGAAAG AGGAAAATGG AACAATGGGG 
GTTTCAAAGC ACAAAAGTGA AAGTCCATGT GAGTCTCCTT ATCCAAATGA GAAAGACAAG 
GAAAAAAATA AGTCAAAATC TTCAGGCAAA GAAAAAGGCG GCGACTCATT TAAATCTGAA 
AAGATGGATA AAATCTCCTC TGGTGGCAAA AAGGAGTCCA GGCATGATAA AGAAAAGATA 
GAAAAGAAAG AGAAACGGGA CAGTTCAGGA GGAAAGGAAG AGAAGAAACA TCATAAGTCC 
TCGGACAAGC ACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_025276464.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025132249.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X16, mRNA.

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CloneID	OBu73761
Clone ID Related Accession (Same CDS sequence)	XM_025276464.1
Accession Version	XM_025276464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAC ACAAACGAGA AATTGTAGAA GCTAAGCAGA TCGTTAGAAA ACTTACAATG 
GTTGTATTGT CTTCTGATAA AATTGACGAG CGAGAGAAAG AAAAGGAAAA AGAAGAGGAG 
AAAGTGGAGA AGCCACCTGA CAACCAAAAA CTTGGTTTGT TGGAAGCTTT GTTAAAGATT 
GGTGATTGGC AGCATGCGCA GAGCATTATG GATCAGATGC CTCCATACTA TGCAGCTTCA 
CATAAACTAA TAGCCCTTGC TATTTGCAAG CTGATTCATA TAACTATTGA GCCTCTCTAT 
CGAAGAGTTG GCGTTCCTAA AGGTGCTAAA GGCTCACCTG TTAGTGCTTT GCAAAATAAG 
AGAGCACCAA AACAAGCAGA GAGCTTTGAA GATTTGAGGA GAGACGTCTT CAATATGTTC 
TGTTACCTTG GTCCCCACCT TTCTCATGAT CCCATTTTAT TCGCAAAAGT GGTGCGGATA 
GGCAAGTCAT TTATGAAGGA GTTTCAGTCT GATGGAAGCA AACAAGAAGA TAAAGAAAAA 
ACGGAAGTTA TCCTTAGCTG TTTGCTTAGC ATTACTGACC AGGTATTACT TCCATCTCTT 
TCTTTGATGG ACTGCAATGC TTGTATGTCT GAGGAACTAT GGGGAATGTT TAAAACATTT 
CCATATCAGC ATAGATATCG TCTGTATGGC CAGTGGAAGA ATGAGACTTA CAACAGTCAT 
CCACTTTTAG TAAAAGTTAA AGCTCAAACA ATAGACAGAG CCAAATATAT CATGAAGCGT 
CTAACCAAGG AAAATGTGAA GCCTTCTGGA AGACAAATTG GGAAGTTGAG CCACAGCAAT 
CCAACCATTT TGTTTGATTA TGTATGTTTT GAGATCTTGT CACAAATACA GAAGTATGAT 
AACTTGATAA CACCTGTAGT AGATTCATTG AAATACCTCA CTTCGTTGAA TTATGACGTC 
TTGGCCTATT GTATCATTGA AGCTTTAGCT AATCCAGAAA AGGAGAAAAT GAAACATGAT 
GACACAACCA TCTCAAGTTG GCTTCAGAGT CTGGCTAGTT TCTGTGGTGC AGTTTTTCGT 
AAATATCCAA TTGATCTTGC TGGTCTTCTT CAGTATGTGG CTAATCAGCT AAAGGCAGGC 
AAAAGTTTTG ACCTGCTTAT ACTGAAAGAA GTGGTACAAA AAATGGCAGG AATAGAAATT 
ACAGAAGAAA TGACAATGGA GCAACTAGAA GCCATGACTG GTGGAGAGCA ACTAAAAGCT 
GAGGGTGGTT ATTTTGGCCA GATAAGAAAC ACGAAAAAAT CCTCCCAGAG ATTAAAGGAT 
GCACTATTAG ACCATGATCT TGCTCTTCCT CTCTGCTTGC TTATGGCTCA GCAGAGGAAT 
GGGGTAATCT TTCAGGAAGG TGGAGAGAAA CATTTGAAAC TTGTGGGAAA ACTCTATGAT 
CAGTGTCATG ATACCCTGGT ACAGTTTGGT GGGTTTTTAG CATCTAATCT AAGCACAGAA 
GATTATATAA AGCGAGTGCC TTCAATTGAT GTGCTCTGTA ATGAATTTCA CACACCTCAT 
GATGCAGCAT TTTTCCTGTC TAGGCCAATG TATGCACACC ATATTTCGTC AAAGTATGAC 
GAACTTAAAA AATCAGAGAA GGGAAGTAAA CAGCAGCATA AAGTTCATAA GTACATCACA 
TCATGTGAAA TGGTGATGGC TCCTGTTCAT GAAGCAGTGG TTTCCTTACA CGTTTCCAAA 
GTCTGGGATG ACATCAGTCC TCAATTCTAT GCCACATTCT GGTCATTGAC AATGTATGAC 
CTTGCAGTTC CACATACCAG CTATGAACGG GAAGTCAATA AACTTAAAAT CCAGATGAAA 
GCAATTGATG ACAATCAGGA AATGCCTCCA AATAAAAAGA AAAAAGAGAA GGAGCGATGT 
ACTGCCCTTC AGGACAAGCT TCTCGAAGAA GAAAAGAAAC AGATGGAACA TGTACAGCGA 
GTTCTGCAGA GACTGAAACT GGAAAAGGAC AACTGGCTTT TAGCAAAATC TACCAAAAAT 
GAGACCATCA CAAAATTTCT ACAGCTGTGT ATATTTCCTC GATGTATTTT TTCAGCAATT 
GATGCTGTTT ACTGTGCTCG TTTTGTTGAA TTGGTACATC AACAGAAAAC TCCAAATTTT 
TCCACACTTC TTTGCTATGA TCGAGTTTTC TCTGATATAA TTTACACAGT TGCAAGCTGT 
ACTGAAAATG AAGCTAGTCG ATATGGGAGA TTCCTTTGCT GCATGTTAGA GACTGTGACC 
AGGTGGCACA GTGATAGAGC CACATATGAA AAGGAATGTG GAAATTATCC AGGATTCCTT 
ACTATATTAC GAGCAACTGG ATTTGATGGT GGAAATAAAG CTGATCAATT AGACTATGAA 
AATTTTCGAC ATGTTGTACA CAAATGGCAT TACAAACTAA CCAAGGCCTC GGTACATTGC 
CTTGAAACAG GCGAATATAC TCACATCAGG AATATCTTGA TTGTGCTAAC AAAAATACTT 
CCTTGGTACC CAAAAGTTTT GAATCTGGGT CAGGCATTGG AAAGAAGAGT GCATAAAATC 
TGCCAAGAGG AAAAAGAGAA GAGGCCAGAT CTATATGCAT TGGCTATGGG CTACTCTGGG 
CAGTTGAAAA GTAGAAAGTC ATACATGATA CCTGAAAATG AGTTTCATCA CAAAGACCCC 
CCTCCGAGGA ATGCAGTTGC CAGTGTACAA AATGGGCCTG GTGGTGGGCC TTCTTCATCG 
TCGATCGGAA GTGTATCTAA GTCAGACGAA AGCAGTACCG AGGACACTGA TAAATCAAGG 
GAGAGATCTC AGTGTGGTGT GAAAGCTGTT AATAAAGCTT CTAGTGCCAC ACCAAAAGGG 
AATTCAAGTA ATGGAAATAG TGGCTCTAAC AGCAGCAAAA CTGTTAAAGA AAATGACAAA 
GAAAAAGGAA AAGAGAAAGA AAAAGAGAAA AAAGAAAAGA CTCCAGCTAC TACTCCAGAG 
GCCCGAGTAC TTGGTAAAGA TGGTAAAGAA AAACCAAAGG AAGAACGGCC AAATAAAGAT 
GAAAAAGCAA GAGAGACAAA GGAAAGAACA CCTAAATCTG ACAAAGAGAA AGAAAAATTC 
AAGAAGGAAG AAAAAGCTAA AGATGAGAAA TTCAAGACCA CTGTCCCGAA TGTAGAATCA 
AAGTCAACTC AAGAAAAGGA AAGAGAGAAG GAGCCGTCCA GAGAACGAGA TATAGCAAAG 
GAAATGAAAT CAAAGGAAAA TGTTAAAGGA GGAGAGAAAA CACCAGTTTC TGGGTCCTTG 
AAATCACCTG TTCCCCGATC AGATATTACA GAGCCTGAAA GGGAACAGAA ACGTCGCAAA 
ATTGATACCC ATCCTTCTCC ATCACATTCC TCAACAGTAA AGATGGTTTA TTTCCAGGTT 
ACAGCCATAC TTCCCAAAGT TCCTCTGGGT TCTGAGAACT ATGCCAGCTC ACCTGTCATC 
TCCATTCATT TTCTACAGGA CAGTCTCATC GAACTCAAGG AGTCTTCAGC AAAGCTCTAC 
ATTAATCATA CTCCTCCACC ACTGTCCAAG AGTAAGGAGA GAGAAATGGA CAAGAAAGAT 
TTGGACAAGT CAAGGGAAAG ATCCAGAGAA AGAGAAAAAA AAGATGAAAA GGACAGGAAA 
GAGCGGAAAA GGGATCACTC AAACAATGAC CGAGAAGTGC CACCGGACTT AACCAAGAGG 
CGGAAAGAGG AAAATGGAAC AATGGGGGTT TCAAAGCACA AAAGTGAAAG TCCATGTGAG 
TCTCCTTATC CAAATGAGAA AGACAAGGAA AAAAATAAGT CAAAATCTTC AGGCAAAGAA 
AAAGGCGGCG ACTCATTTAA ATCTGAAAAG ATGGATAAAA TCTCCTCTGG TGGCAAAAAG 
GAGTCCAGGC ATGATAAAGA AAAGATAGAA AAGAAAGAGA AACGGGACAG TTCAGGAGGA 
AAGGAAGAGA AGAAACATCA TAAGTCCTCG GACAAGCACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025132249.1
CDS	413..4417
Translation	MDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYVCFEILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKARETKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKMVYFQVTAILPKVPLGSENYASSPVISIHFLQDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKHHKSSDKHR

Target ORF information:

RefSeq Version	XM_025276464.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025276464.1

ORF Insert Sequence:

ATGGATGAAC ACAAACGAGA AATTGTAGAA GCTAAGCAGA TCGTTAGAAA ACTTACAATG 
GTTGTATTGT CTTCTGATAA AATTGACGAG CGAGAGAAAG AAAAGGAAAA AGAAGAGGAG 
AAAGTGGAGA AGCCACCTGA CAACCAAAAA CTTGGTTTGT TGGAAGCTTT GTTAAAGATT 
GGTGATTGGC AGCATGCGCA GAGCATTATG GATCAGATGC CTCCATACTA TGCAGCTTCA 
CATAAACTAA TAGCCCTTGC TATTTGCAAG CTGATTCATA TAACTATTGA GCCTCTCTAT 
CGAAGAGTTG GCGTTCCTAA AGGTGCTAAA GGCTCACCTG TTAGTGCTTT GCAAAATAAG 
AGAGCACCAA AACAAGCAGA GAGCTTTGAA GATTTGAGGA GAGACGTCTT CAATATGTTC 
TGTTACCTTG GTCCCCACCT TTCTCATGAT CCCATTTTAT TCGCAAAAGT GGTGCGGATA 
GGCAAGTCAT TTATGAAGGA GTTTCAGTCT GATGGAAGCA AACAAGAAGA TAAAGAAAAA 
ACGGAAGTTA TCCTTAGCTG TTTGCTTAGC ATTACTGACC AGGTATTACT TCCATCTCTT 
TCTTTGATGG ACTGCAATGC TTGTATGTCT GAGGAACTAT GGGGAATGTT TAAAACATTT 
CCATATCAGC ATAGATATCG TCTGTATGGC CAGTGGAAGA ATGAGACTTA CAACAGTCAT 
CCACTTTTAG TAAAAGTTAA AGCTCAAACA ATAGACAGAG CCAAATATAT CATGAAGCGT 
CTAACCAAGG AAAATGTGAA GCCTTCTGGA AGACAAATTG GGAAGTTGAG CCACAGCAAT 
CCAACCATTT TGTTTGATTA TGTATGTTTT GAGATCTTGT CACAAATACA GAAGTATGAT 
AACTTGATAA CACCTGTAGT AGATTCATTG AAATACCTCA CTTCGTTGAA TTATGACGTC 
TTGGCCTATT GTATCATTGA AGCTTTAGCT AATCCAGAAA AGGAGAAAAT GAAACATGAT 
GACACAACCA TCTCAAGTTG GCTTCAGAGT CTGGCTAGTT TCTGTGGTGC AGTTTTTCGT 
AAATATCCAA TTGATCTTGC TGGTCTTCTT CAGTATGTGG CTAATCAGCT AAAGGCAGGC 
AAAAGTTTTG ACCTGCTTAT ACTGAAAGAA GTGGTACAAA AAATGGCAGG AATAGAAATT 
ACAGAAGAAA TGACAATGGA GCAACTAGAA GCCATGACTG GTGGAGAGCA ACTAAAAGCT 
GAGGGTGGTT ATTTTGGCCA GATAAGAAAC ACGAAAAAAT CCTCCCAGAG ATTAAAGGAT 
GCACTATTAG ACCATGATCT TGCTCTTCCT CTCTGCTTGC TTATGGCTCA GCAGAGGAAT 
GGGGTAATCT TTCAGGAAGG TGGAGAGAAA CATTTGAAAC TTGTGGGAAA ACTCTATGAT 
CAGTGTCATG ATACCCTGGT ACAGTTTGGT GGGTTTTTAG CATCTAATCT AAGCACAGAA 
GATTATATAA AGCGAGTGCC TTCAATTGAT GTGCTCTGTA ATGAATTTCA CACACCTCAT 
GATGCAGCAT TTTTCCTGTC TAGGCCAATG TATGCACACC ATATTTCGTC AAAGTATGAC 
GAACTTAAAA AATCAGAGAA GGGAAGTAAA CAGCAGCATA AAGTTCATAA GTACATCACA 
TCATGTGAAA TGGTGATGGC TCCTGTTCAT GAAGCAGTGG TTTCCTTACA CGTTTCCAAA 
GTCTGGGATG ACATCAGTCC TCAATTCTAT GCCACATTCT GGTCATTGAC AATGTATGAC 
CTTGCAGTTC CACATACCAG CTATGAACGG GAAGTCAATA AACTTAAAAT CCAGATGAAA 
GCAATTGATG ACAATCAGGA AATGCCTCCA AATAAAAAGA AAAAAGAGAA GGAGCGATGT 
ACTGCCCTTC AGGACAAGCT TCTCGAAGAA GAAAAGAAAC AGATGGAACA TGTACAGCGA 
GTTCTGCAGA GACTGAAACT GGAAAAGGAC AACTGGCTTT TAGCAAAATC TACCAAAAAT 
GAGACCATCA CAAAATTTCT ACAGCTGTGT ATATTTCCTC GATGTATTTT TTCAGCAATT 
GATGCTGTTT ACTGTGCTCG TTTTGTTGAA TTGGTACATC AACAGAAAAC TCCAAATTTT 
TCCACACTTC TTTGCTATGA TCGAGTTTTC TCTGATATAA TTTACACAGT TGCAAGCTGT 
ACTGAAAATG AAGCTAGTCG ATATGGGAGA TTCCTTTGCT GCATGTTAGA GACTGTGACC 
AGGTGGCACA GTGATAGAGC CACATATGAA AAGGAATGTG GAAATTATCC AGGATTCCTT 
ACTATATTAC GAGCAACTGG ATTTGATGGT GGAAATAAAG CTGATCAATT AGACTATGAA 
AATTTTCGAC ATGTTGTACA CAAATGGCAT TACAAACTAA CCAAGGCCTC GGTACATTGC 
CTTGAAACAG GCGAATATAC TCACATCAGG AATATCTTGA TTGTGCTAAC AAAAATACTT 
CCTTGGTACC CAAAAGTTTT GAATCTGGGT CAGGCATTGG AAAGAAGAGT GCATAAAATC 
TGCCAAGAGG AAAAAGAGAA GAGGCCAGAT CTATATGCAT TGGCTATGGG CTACTCTGGG 
CAGTTGAAAA GTAGAAAGTC ATACATGATA CCTGAAAATG AGTTTCATCA CAAAGACCCC 
CCTCCGAGGA ATGCAGTTGC CAGTGTACAA AATGGGCCTG GTGGTGGGCC TTCTTCATCG 
TCGATCGGAA GTGTATCTAA GTCAGACGAA AGCAGTACCG AGGACACTGA TAAATCAAGG 
GAGAGATCTC AGTGTGGTGT GAAAGCTGTT AATAAAGCTT CTAGTGCCAC ACCAAAAGGG 
AATTCAAGTA ATGGAAATAG TGGCTCTAAC AGCAGCAAAA CTGTTAAAGA AAATGACAAA 
GAAAAAGGAA AAGAGAAAGA AAAAGAGAAA AAAGAAAAGA CTCCAGCTAC TACTCCAGAG 
GCCCGAGTAC TTGGTAAAGA TGGTAAAGAA AAACCAAAGG AAGAACGGCC AAATAAAGAT 
GAAAAAGCAA GAGAGACAAA GGAAAGAACA CCTAAATCTG ACAAAGAGAA AGAAAAATTC 
AAGAAGGAAG AAAAAGCTAA AGATGAGAAA TTCAAGACCA CTGTCCCGAA TGTAGAATCA 
AAGTCAACTC AAGAAAAGGA AAGAGAGAAG GAGCCGTCCA GAGAACGAGA TATAGCAAAG 
GAAATGAAAT CAAAGGAAAA TGTTAAAGGA GGAGAGAAAA CACCAGTTTC TGGGTCCTTG 
AAATCACCTG TTCCCCGATC AGATATTACA GAGCCTGAAA GGGAACAGAA ACGTCGCAAA 
ATTGATACCC ATCCTTCTCC ATCACATTCC TCAACAGTAA AGATGGTTTA TTTCCAGGTT 
ACAGCCATAC TTCCCAAAGT TCCTCTGGGT TCTGAGAACT ATGCCAGCTC ACCTGTCATC 
TCCATTCATT TTCTACAGGA CAGTCTCATC GAACTCAAGG AGTCTTCAGC AAAGCTCTAC 
ATTAATCATA CTCCTCCACC ACTGTCCAAG AGTAAGGAGA GAGAAATGGA CAAGAAAGAT 
TTGGACAAGT CAAGGGAAAG ATCCAGAGAA AGAGAAAAAA AAGATGAAAA GGACAGGAAA 
GAGCGGAAAA GGGATCACTC AAACAATGAC CGAGAAGTGC CACCGGACTT AACCAAGAGG 
CGGAAAGAGG AAAATGGAAC AATGGGGGTT TCAAAGCACA AAAGTGAAAG TCCATGTGAG 
TCTCCTTATC CAAATGAGAA AGACAAGGAA AAAAATAAGT CAAAATCTTC AGGCAAAGAA 
AAAGGCGGCG ACTCATTTAA ATCTGAAAAG ATGGATAAAA TCTCCTCTGG TGGCAAAAAG 
GAGTCCAGGC ATGATAAAGA AAAGATAGAA AAGAAAGAGA AACGGGACAG TTCAGGAGGA 
AAGGAAGAGA AGAAACATCA TAAGTCCTCG GACAAGCACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_006064991.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065053.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu20606
Clone ID Related Accession (Same CDS sequence)	XM_006064991.1
Accession Version	XM_006064991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785155.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAT GAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGTTAC AGCCATACTT CCCAAAGTTC CTCTGGGTTC TGAGAACTAT 
GCCAGCTCAC CTGTCATCTC CATTCATTTT CTACAGGACA GTCTCATCGA ACTCAAGGAG 
TCTTCAGCAA AGCTCTACAT TAATCATACT CCTCCACCAC TGTCCAAGAG TAAGGAGAGA 
GAAATGGACA AGAAAGATTT GGACAAGTCA AGGGAAAGAT CCAGAGAAAG AGAAAAAAAA 
GATGAAAAGG ACAGGAAAGA GCGGAAAAGG GATCACTCAA ACAATGACCG AGAAGTGCCA 
CCGGACTTAA CCAAGAGGCG GAAAGAGGAA AATGGAACAA TGGGGGTTTC AAAGCACAAA 
AGTGAAAGTC CATGTGAGTC TCCTTATCCA AATGAGAAAG ACAAGGAAAA AAATAAGTCA 
AAATCTTCAG GCAAAGAAAA AGGCGGCGAC TCATTTAAAT CTGAAAAGAT GGATAAAATC 
TCCTCTGGTG GCAAAAAGGA GTCCAGGCAT GATAAAGAAA AGATAGAAAA GAAAGAGAAA 
CGGGACAGTT CAGGAGGAAA GGAAGAGAAG AAACATCATA AGTCCTCGGA CAAGCACAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065053.1
CDS	39..4901
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKAREMKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKVTAILPKVPLGSENYASSPVISIHFLQDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKHHKSSDKHR

Target ORF information:

RefSeq Version	XM_006064991.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064991.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAT GAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGTTAC AGCCATACTT CCCAAAGTTC CTCTGGGTTC TGAGAACTAT 
GCCAGCTCAC CTGTCATCTC CATTCATTTT CTACAGGACA GTCTCATCGA ACTCAAGGAG 
TCTTCAGCAA AGCTCTACAT TAATCATACT CCTCCACCAC TGTCCAAGAG TAAGGAGAGA 
GAAATGGACA AGAAAGATTT GGACAAGTCA AGGGAAAGAT CCAGAGAAAG AGAAAAAAAA 
GATGAAAAGG ACAGGAAAGA GCGGAAAAGG GATCACTCAA ACAATGACCG AGAAGTGCCA 
CCGGACTTAA CCAAGAGGCG GAAAGAGGAA AATGGAACAA TGGGGGTTTC AAAGCACAAA 
AGTGAAAGTC CATGTGAGTC TCCTTATCCA AATGAGAAAG ACAAGGAAAA AAATAAGTCA 
AAATCTTCAG GCAAAGAAAA AGGCGGCGAC TCATTTAAAT CTGAAAAGAT GGATAAAATC 
TCCTCTGGTG GCAAAAAGGA GTCCAGGCAT GATAAAGAAA AGATAGAAAA GAAAGAGAAA 
CGGGACAGTT CAGGAGGAAA GGAAGAGAAG AAACATCATA AGTCCTCGGA CAAGCACAGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_006064992.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065054.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu20607
Clone ID Related Accession (Same CDS sequence)	XM_006064992.1
Accession Version	XM_006064992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785155.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAAAA CTGTTAAAGA AAATGACAAA GAAAAAGGAA AAGAGAAAGA AAAAGAGAAA 
AAAGAAAAGA CTCCAGCTAC TACTCCAGAG GCCCGAGTAC TTGGTAAAGA TGGTAAAGAA 
AAACCAAAGG AAGAACGGCC AAATAAAGAT GAAAAAGCAA GAGAGATGAA GGAAAGAACA 
CCTAAATCTG ACAAAGAGAA AGAAAAATTC AAGAAGGAAG AAAAAGCTAA AGATGAGAAA 
TTCAAGACCA CTGTCCCGAA TGTAGAATCA AAGTCAACTC AAGAAAAGGA AAGAGAGAAG 
GAGCCGTCCA GAGAACGAGA TATAGCAAAG GAAATGAAAT CAAAGGAAAA TGTTAAAGGA 
GGAGAGAAAA CACCAGTTTC TGGGTCCTTG AAATCACCTG TTCCCCGATC AGATATTACA 
GAGCCTGAAA GGGAACAGAA ACGTCGCAAA ATTGATACCC ATCCTTCTCC ATCACATTCC 
TCAACAGTAA AGGTTACAGC CATACTTCCC AAAGTTCCTC TGGGTTCTGA GAACTATGCC 
AGCTCACCTG TCATCTCCAT TCATTTTCTA CAGGACAGTC TCATCGAACT CAAGGAGTCT 
TCAGCAAAGC TCTACATTAA TCATACTCCT CCACCACTGT CCAAGAGTAA GGAGAGAGAA 
ATGGACAAGA AAGATTTGGA CAAGTCAAGG GAAAGATCCA GAGAAAGAGA AAAAAAAGAT 
GAAAAGGACA GGAAAGAGCG GAAAAGGGAT CACTCAAACA ATGACCGAGA AGTGCCACCG 
GACTTAACCA AGAGGCGGAA AGAGGAAAAT GGAACAATGG GGGTTTCAAA GCACAAAAGT 
GAAAGTCCAT GTGAGTCTCC TTATCCAAAT GAGAAAGACA AGGAAAAAAA TAAGTCAAAA 
TCTTCAGGCA AAGAAAAAGG CGGCGACTCA TTTAAATCTG AAAAGATGGA TAAAATCTCC 
TCTGGTGGCA AAAAGGAGTC CAGGCATGAT AAAGAAAAGA TAGAAAAGAA AGAGAAACGG 
GACAGTTCAG GAGGAAAGGA AGAGAAGAAA CATCATAAGT CCTCGGACAA GCACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065054.1
CDS	39..4898
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKAREMKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKVTAILPKVPLGSENYASSPVISIHFLQDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKHHKSSDKHR

Target ORF information:

RefSeq Version	XM_006064992.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064992.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAAAA CTGTTAAAGA AAATGACAAA GAAAAAGGAA AAGAGAAAGA AAAAGAGAAA 
AAAGAAAAGA CTCCAGCTAC TACTCCAGAG GCCCGAGTAC TTGGTAAAGA TGGTAAAGAA 
AAACCAAAGG AAGAACGGCC AAATAAAGAT GAAAAAGCAA GAGAGATGAA GGAAAGAACA 
CCTAAATCTG ACAAAGAGAA AGAAAAATTC AAGAAGGAAG AAAAAGCTAA AGATGAGAAA 
TTCAAGACCA CTGTCCCGAA TGTAGAATCA AAGTCAACTC AAGAAAAGGA AAGAGAGAAG 
GAGCCGTCCA GAGAACGAGA TATAGCAAAG GAAATGAAAT CAAAGGAAAA TGTTAAAGGA 
GGAGAGAAAA CACCAGTTTC TGGGTCCTTG AAATCACCTG TTCCCCGATC AGATATTACA 
GAGCCTGAAA GGGAACAGAA ACGTCGCAAA ATTGATACCC ATCCTTCTCC ATCACATTCC 
TCAACAGTAA AGGTTACAGC CATACTTCCC AAAGTTCCTC TGGGTTCTGA GAACTATGCC 
AGCTCACCTG TCATCTCCAT TCATTTTCTA CAGGACAGTC TCATCGAACT CAAGGAGTCT 
TCAGCAAAGC TCTACATTAA TCATACTCCT CCACCACTGT CCAAGAGTAA GGAGAGAGAA 
ATGGACAAGA AAGATTTGGA CAAGTCAAGG GAAAGATCCA GAGAAAGAGA AAAAAAAGAT 
GAAAAGGACA GGAAAGAGCG GAAAAGGGAT CACTCAAACA ATGACCGAGA AGTGCCACCG 
GACTTAACCA AGAGGCGGAA AGAGGAAAAT GGAACAATGG GGGTTTCAAA GCACAAAAGT 
GAAAGTCCAT GTGAGTCTCC TTATCCAAAT GAGAAAGACA AGGAAAAAAA TAAGTCAAAA 
TCTTCAGGCA AAGAAAAAGG CGGCGACTCA TTTAAATCTG AAAAGATGGA TAAAATCTCC 
TCTGGTGGCA AAAAGGAGTC CAGGCATGAT AAAGAAAAGA TAGAAAAGAA AGAGAAACGG 
GACAGTTCAG GAGGAAAGGA AGAGAAGAAA CATCATAAGT CCTCGGACAA GCACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_006064993.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065055.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X3, mRNA.

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CloneID	OBu20608
Clone ID Related Accession (Same CDS sequence)	XM_006064993.1
Accession Version	XM_006064993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785155.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAT GAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGTTAC AGCCATACTT CCCAAAGTTC CTCTGGGTTC TGAGAACTAT 
GCCAGCTCAC CTGTCATCTC CATTCATTTT CTACAGGACA GTCTCATCGA ACTCAAGGAG 
TCTTCAGCAA AGCTCTACAT TAATCATACT CCTCCACCAC TGTCCAAGAG TAAGGAGAGA 
GAAATGGACA AGAAAGATTT GGACAAGTCA AGGGAAAGAT CCAGAGAAAG AGAAAAAAAA 
GATGAAAAGG ACAGGAAAGA GCGGAAAAGG GATCACTCAA ACAATGACCG AGAAGTGCCA 
CCGGACTTAA CCAAGAGGCG GAAAGAGGAA AATGGAACAA TGGGGGTTTC AAAGCACAAA 
AGTGAAAGTC CATGTGAGTC TCCTTATCCA AATGAGAAAG ACAAGGAAAA AAATAAGTCA 
AAATCTTCAG GCAAAGAAAA AGGCGGCGAC TCATTTAAAT CTGAAAAGAT GGATAAAATC 
TCCTCTGGTG GCAAAAAGGA GTCCAGGCAT GATAAAGAAA AGATAGAAAA GAAAGAGAAA 
CGGGACAGTT CAGGAGGAAA GGAAGAGAAG AAACAGTCCT CGGACAAGCA CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065055.1
CDS	39..4895
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKAREMKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKVTAILPKVPLGSENYASSPVISIHFLQDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKQSSDKHR

Target ORF information:

RefSeq Version	XM_006064993.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064993.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAT GAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGTTAC AGCCATACTT CCCAAAGTTC CTCTGGGTTC TGAGAACTAT 
GCCAGCTCAC CTGTCATCTC CATTCATTTT CTACAGGACA GTCTCATCGA ACTCAAGGAG 
TCTTCAGCAA AGCTCTACAT TAATCATACT CCTCCACCAC TGTCCAAGAG TAAGGAGAGA 
GAAATGGACA AGAAAGATTT GGACAAGTCA AGGGAAAGAT CCAGAGAAAG AGAAAAAAAA 
GATGAAAAGG ACAGGAAAGA GCGGAAAAGG GATCACTCAA ACAATGACCG AGAAGTGCCA 
CCGGACTTAA CCAAGAGGCG GAAAGAGGAA AATGGAACAA TGGGGGTTTC AAAGCACAAA 
AGTGAAAGTC CATGTGAGTC TCCTTATCCA AATGAGAAAG ACAAGGAAAA AAATAAGTCA 
AAATCTTCAG GCAAAGAAAA AGGCGGCGAC TCATTTAAAT CTGAAAAGAT GGATAAAATC 
TCCTCTGGTG GCAAAAAGGA GTCCAGGCAT GATAAAGAAA AGATAGAAAA GAAAGAGAAA 
CGGGACAGTT CAGGAGGAAA GGAAGAGAAG AAACAGTCCT CGGACAAGCA CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_006064994.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065056.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X4, mRNA.

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CloneID	OBu20609
Clone ID Related Accession (Same CDS sequence)	XM_006064994.1
Accession Version	XM_006064994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785155.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAT GAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGACAG TCTCATCGAA CTCAAGGAGT CTTCAGCAAA GCTCTACATT 
AATCATACTC CTCCACCACT GTCCAAGAGT AAGGAGAGAG AAATGGACAA GAAAGATTTG 
GACAAGTCAA GGGAAAGATC CAGAGAAAGA GAAAAAAAAG ATGAAAAGGA CAGGAAAGAG 
CGGAAAAGGG ATCACTCAAA CAATGACCGA GAAGTGCCAC CGGACTTAAC CAAGAGGCGG 
AAAGAGGAAA ATGGAACAAT GGGGGTTTCA AAGCACAAAA GTGAAAGTCC ATGTGAGTCT 
CCTTATCCAA ATGAGAAAGA CAAGGAAAAA AATAAGTCAA AATCTTCAGG CAAAGAAAAA 
GGCGGCGACT CATTTAAATC TGAAAAGATG GATAAAATCT CCTCTGGTGG CAAAAAGGAG 
TCCAGGCATG ATAAAGAAAA GATAGAAAAG AAAGAGAAAC GGGACAGTTC AGGAGGAAAG 
GAAGAGAAGA AACAATATCC TTTTCATCTT ATAGATCTTT TTAATCAGTA TAATGGAAAA 
CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065056.1
CDS	39..4844
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKAREMKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKQYPFHLIDLFNQYNGKL

Target ORF information:

RefSeq Version	XM_006064994.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064994.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAT GAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGACAG TCTCATCGAA CTCAAGGAGT CTTCAGCAAA GCTCTACATT 
AATCATACTC CTCCACCACT GTCCAAGAGT AAGGAGAGAG AAATGGACAA GAAAGATTTG 
GACAAGTCAA GGGAAAGATC CAGAGAAAGA GAAAAAAAAG ATGAAAAGGA CAGGAAAGAG 
CGGAAAAGGG ATCACTCAAA CAATGACCGA GAAGTGCCAC CGGACTTAAC CAAGAGGCGG 
AAAGAGGAAA ATGGAACAAT GGGGGTTTCA AAGCACAAAA GTGAAAGTCC ATGTGAGTCT 
CCTTATCCAA ATGAGAAAGA CAAGGAAAAA AATAAGTCAA AATCTTCAGG CAAAGAAAAA 
GGCGGCGACT CATTTAAATC TGAAAAGATG GATAAAATCT CCTCTGGTGG CAAAAAGGAG 
TCCAGGCATG ATAAAGAAAA GATAGAAAAG AAAGAGAAAC GGGACAGTTC AGGAGGAAAG 
GAAGAGAAGA AACAATATCC TTTTCATCTT ATAGATCTTT TTAATCAGTA TAATGGAAAA 
CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_006064995.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065057.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X5, mRNA.

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CloneID	OBu20610
Clone ID Related Accession (Same CDS sequence)	XM_006064995.1
Accession Version	XM_006064995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785155.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAAAA CTGTTAAAGA AAATGACAAA GAAAAAGGAA AAGAGAAAGA AAAAGAGAAA 
AAAGAAAAGA CTCCAGCTAC TACTCCAGAG GCCCGAGTAC TTGGTAAAGA TGGTAAAGAA 
AAACCAAAGG AAGAACGGCC AAATAAAGAT GAAAAAGCAA GAGAGATGAA GGAAAGAACA 
CCTAAATCTG ACAAAGAGAA AGAAAAATTC AAGAAGGAAG AAAAAGCTAA AGATGAGAAA 
TTCAAGACCA CTGTCCCGAA TGTAGAATCA AAGTCAACTC AAGAAAAGGA AAGAGAGAAG 
GAGCCGTCCA GAGAACGAGA TATAGCAAAG GAAATGAAAT CAAAGGAAAA TGTTAAAGGA 
GGAGAGAAAA CACCAGTTTC TGGGTCCTTG AAATCACCTG TTCCCCGATC AGATATTACA 
GAGCCTGAAA GGGAACAGAA ACGTCGCAAA ATTGATACCC ATCCTTCTCC ATCACATTCC 
TCAACAGTAA AGGACAGTCT CATCGAACTC AAGGAGTCTT CAGCAAAGCT CTACATTAAT 
CATACTCCTC CACCACTGTC CAAGAGTAAG GAGAGAGAAA TGGACAAGAA AGATTTGGAC 
AAGTCAAGGG AAAGATCCAG AGAAAGAGAA AAAAAAGATG AAAAGGACAG GAAAGAGCGG 
AAAAGGGATC ACTCAAACAA TGACCGAGAA GTGCCACCGG ACTTAACCAA GAGGCGGAAA 
GAGGAAAATG GAACAATGGG GGTTTCAAAG CACAAAAGTG AAAGTCCATG TGAGTCTCCT 
TATCCAAATG AGAAAGACAA GGAAAAAAAT AAGTCAAAAT CTTCAGGCAA AGAAAAAGGC 
GGCGACTCAT TTAAATCTGA AAAGATGGAT AAAATCTCCT CTGGTGGCAA AAAGGAGTCC 
AGGCATGATA AAGAAAAGAT AGAAAAGAAA GAGAAACGGG ACAGTTCAGG AGGAAAGGAA 
GAGAAGAAAC AATATCCTTT TCATCTTATA GATCTTTTTA ATCAGTATAA TGGAAAACTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065057.1
CDS	39..4841
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKAREMKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKQYPFHLIDLFNQYNGKL

Target ORF information:

RefSeq Version	XM_006064995.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064995.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAAAA CTGTTAAAGA AAATGACAAA GAAAAAGGAA AAGAGAAAGA AAAAGAGAAA 
AAAGAAAAGA CTCCAGCTAC TACTCCAGAG GCCCGAGTAC TTGGTAAAGA TGGTAAAGAA 
AAACCAAAGG AAGAACGGCC AAATAAAGAT GAAAAAGCAA GAGAGATGAA GGAAAGAACA 
CCTAAATCTG ACAAAGAGAA AGAAAAATTC AAGAAGGAAG AAAAAGCTAA AGATGAGAAA 
TTCAAGACCA CTGTCCCGAA TGTAGAATCA AAGTCAACTC AAGAAAAGGA AAGAGAGAAG 
GAGCCGTCCA GAGAACGAGA TATAGCAAAG GAAATGAAAT CAAAGGAAAA TGTTAAAGGA 
GGAGAGAAAA CACCAGTTTC TGGGTCCTTG AAATCACCTG TTCCCCGATC AGATATTACA 
GAGCCTGAAA GGGAACAGAA ACGTCGCAAA ATTGATACCC ATCCTTCTCC ATCACATTCC 
TCAACAGTAA AGGACAGTCT CATCGAACTC AAGGAGTCTT CAGCAAAGCT CTACATTAAT 
CATACTCCTC CACCACTGTC CAAGAGTAAG GAGAGAGAAA TGGACAAGAA AGATTTGGAC 
AAGTCAAGGG AAAGATCCAG AGAAAGAGAA AAAAAAGATG AAAAGGACAG GAAAGAGCGG 
AAAAGGGATC ACTCAAACAA TGACCGAGAA GTGCCACCGG ACTTAACCAA GAGGCGGAAA 
GAGGAAAATG GAACAATGGG GGTTTCAAAG CACAAAAGTG AAAGTCCATG TGAGTCTCCT 
TATCCAAATG AGAAAGACAA GGAAAAAAAT AAGTCAAAAT CTTCAGGCAA AGAAAAAGGC 
GGCGACTCAT TTAAATCTGA AAAGATGGAT AAAATCTCCT CTGGTGGCAA AAAGGAGTCC 
AGGCATGATA AAGAAAAGAT AGAAAAGAAA GAGAAACGGG ACAGTTCAGG AGGAAAGGAA 
GAGAAGAAAC AATATCCTTT TCATCTTATA GATCTTTTTA ATCAGTATAA TGGAAAACTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_006064997.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065059.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X7, mRNA.

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CloneID	OBu20611
Clone ID Related Accession (Same CDS sequence)	XM_006064997.1 , XM_006064996.1
Accession Version	XM_006064997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785155.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAT GAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGACAG TCTCATCGAA CTCAAGGAGT CTTCAGCAAA GCTCTACATT 
AATCATACTC CTCCACCACT GTCCAAGAGT AAGGAGAGAG AAATGGACAA GAAAGATTTG 
GACAAGTCAA GGGAAAGATC CAGAGAAAGA GAAAAAAAAG ATGAAAAGGA CAGGAAAGAG 
CGGAAAAGGG ATCACTCAAA CAATGACCGA GAAGTGCCAC CGGACTTAAC CAAGAGGCGG 
AAAGAGGAAA ATGGAACAAT GGGGGTTTCA AAGCACAAAA GTGAAAGTCC ATGTGAGTCT 
CCTTATCCAA ATGAGAAAGA CAAGGAAAAA AATAAGTCAA AATCTTCAGG CAAAGAAAAA 
GGCGGCGACT CATTTAAATC TGAAAAGATG GATAAAATCT CCTCTGGTGG CAAAAAGGAG 
TCCAGGCATG ATAAAGAAAA GATAGAAAAG AAAGAGAAAC GGGACAGTTC AGGAGGAAAG 
GAAGAGAAGA AACATCATAA GTCCTCGGAC AAGCACAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065059.1
CDS	39..4820
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKAREMKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKHHKSSDKHR

Target ORF information:

RefSeq Version	XM_006064997.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064997.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAT GAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGACAG TCTCATCGAA CTCAAGGAGT CTTCAGCAAA GCTCTACATT 
AATCATACTC CTCCACCACT GTCCAAGAGT AAGGAGAGAG AAATGGACAA GAAAGATTTG 
GACAAGTCAA GGGAAAGATC CAGAGAAAGA GAAAAAAAAG ATGAAAAGGA CAGGAAAGAG 
CGGAAAAGGG ATCACTCAAA CAATGACCGA GAAGTGCCAC CGGACTTAAC CAAGAGGCGG 
AAAGAGGAAA ATGGAACAAT GGGGGTTTCA AAGCACAAAA GTGAAAGTCC ATGTGAGTCT 
CCTTATCCAA ATGAGAAAGA CAAGGAAAAA AATAAGTCAA AATCTTCAGG CAAAGAAAAA 
GGCGGCGACT CATTTAAATC TGAAAAGATG GATAAAATCT CCTCTGGTGG CAAAAAGGAG 
TCCAGGCATG ATAAAGAAAA GATAGAAAAG AAAGAGAAAC GGGACAGTTC AGGAGGAAAG 
GAAGAGAAGA AACATCATAA GTCCTCGGAC AAGCACAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_006064996.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065058.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBu20611
Clone ID Related Accession (Same CDS sequence)	XM_006064997.1 , XM_006064996.1
Accession Version	XM_006064996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785155.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAT GAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGACAG TCTCATCGAA CTCAAGGAGT CTTCAGCAAA GCTCTACATT 
AATCATACTC CTCCACCACT GTCCAAGAGT AAGGAGAGAG AAATGGACAA GAAAGATTTG 
GACAAGTCAA GGGAAAGATC CAGAGAAAGA GAAAAAAAAG ATGAAAAGGA CAGGAAAGAG 
CGGAAAAGGG ATCACTCAAA CAATGACCGA GAAGTGCCAC CGGACTTAAC CAAGAGGCGG 
AAAGAGGAAA ATGGAACAAT GGGGGTTTCA AAGCACAAAA GTGAAAGTCC ATGTGAGTCT 
CCTTATCCAA ATGAGAAAGA CAAGGAAAAA AATAAGTCAA AATCTTCAGG CAAAGAAAAA 
GGCGGCGACT CATTTAAATC TGAAAAGATG GATAAAATCT CCTCTGGTGG CAAAAAGGAG 
TCCAGGCATG ATAAAGAAAA GATAGAAAAG AAAGAGAAAC GGGACAGTTC AGGAGGAAAG 
GAAGAGAAGA AACATCATAA GTCCTCGGAC AAGCACAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065058.1
CDS	39..4820
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKAREMKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKHHKSSDKHR

Target ORF information:

RefSeq Version	XM_006064996.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064996.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAT GAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGACAG TCTCATCGAA CTCAAGGAGT CTTCAGCAAA GCTCTACATT 
AATCATACTC CTCCACCACT GTCCAAGAGT AAGGAGAGAG AAATGGACAA GAAAGATTTG 
GACAAGTCAA GGGAAAGATC CAGAGAAAGA GAAAAAAAAG ATGAAAAGGA CAGGAAAGAG 
CGGAAAAGGG ATCACTCAAA CAATGACCGA GAAGTGCCAC CGGACTTAAC CAAGAGGCGG 
AAAGAGGAAA ATGGAACAAT GGGGGTTTCA AAGCACAAAA GTGAAAGTCC ATGTGAGTCT 
CCTTATCCAA ATGAGAAAGA CAAGGAAAAA AATAAGTCAA AATCTTCAGG CAAAGAAAAA 
GGCGGCGACT CATTTAAATC TGAAAAGATG GATAAAATCT CCTCTGGTGG CAAAAAGGAG 
TCCAGGCATG ATAAAGAAAA GATAGAAAAG AAAGAGAAAC GGGACAGTTC AGGAGGAAAG 
GAAGAGAAGA AACATCATAA GTCCTCGGAC AAGCACAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_006064998.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065060.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBu20612
Clone ID Related Accession (Same CDS sequence)	XM_006064998.1
Accession Version	XM_006064998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785155.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAT GAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGTTAC AGCCATACTT CCCAAAGTTC CTCTGGGTTC TGAGAACTAT 
GCCAGCTCAC CTGTCATCTC CATTCATTTT CTACAGGACA GTCTCATCGA ACTCAAGGAG 
TCTTCAGCAA AGCTCTACAT TAATCATACT CCTCCACCAC TGTCCAAGAG TAAGGAGAGA 
GAAATGGACA AGAAAGATTT GGACAAGTCA AGGGAAAGAT CCAGAGAAAG AGAAAAAAAA 
GATGAAAAGG ACAGGAAAGA GCGGAAAAGG GATCACTCAA ACAATGACCG AGAAGTGCCA 
CCGGACTTAA CCAAGAGGCG GAAAGAGGAA AATGGAACAA TGGGGGTTTC AAAGCACAAA 
AGTGAAAGTC CATGTGAGTC TCCTTATCCA AATGAGAAAG ACAAGGAAAA AAATAAGTCA 
AAATCTTCAG GCAAAGAAAA AGGCGGCGAC TCATTTAAAT CTGAAAAGAT GGATAAAATC 
TCCTCTGGTG GCAAAAAGGG TGTGGACAGA CCCCTAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065060.1
CDS	39..4820
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKAREMKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKVTAILPKVPLGSENYASSPVISIHFLQDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKGVDRPLS

Target ORF information:

RefSeq Version	XM_006064998.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064998.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAT GAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGTTAC AGCCATACTT CCCAAAGTTC CTCTGGGTTC TGAGAACTAT 
GCCAGCTCAC CTGTCATCTC CATTCATTTT CTACAGGACA GTCTCATCGA ACTCAAGGAG 
TCTTCAGCAA AGCTCTACAT TAATCATACT CCTCCACCAC TGTCCAAGAG TAAGGAGAGA 
GAAATGGACA AGAAAGATTT GGACAAGTCA AGGGAAAGAT CCAGAGAAAG AGAAAAAAAA 
GATGAAAAGG ACAGGAAAGA GCGGAAAAGG GATCACTCAA ACAATGACCG AGAAGTGCCA 
CCGGACTTAA CCAAGAGGCG GAAAGAGGAA AATGGAACAA TGGGGGTTTC AAAGCACAAA 
AGTGAAAGTC CATGTGAGTC TCCTTATCCA AATGAGAAAG ACAAGGAAAA AAATAAGTCA 
AAATCTTCAG GCAAAGAAAA AGGCGGCGAC TCATTTAAAT CTGAAAAGAT GGATAAAATC 
TCCTCTGGTG GCAAAAAGGG TGTGGACAGA CCCCTAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_025276457.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025132242.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X1, mRNA.

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CloneID	OBu73753
Clone ID Related Accession (Same CDS sequence)	XM_025276457.1 , XM_025276458.1
Accession Version	XM_025276457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATGTAT GTTTTGAGAT CTTGTCACAA ATACAGAAGT ATGATAACTT GATAACACCT 
GTAGTAGATT CATTGAAATA CCTCACTTCG TTGAATTATG ACGTCTTGGC CTATTGTATC 
ATTGAAGCTT TAGCTAATCC AGAAAAGGAG AAAATGAAAC ATGATGACAC AACCATCTCA 
AGTTGGCTTC AGAGTCTGGC TAGTTTCTGT GGTGCAGTTT TTCGTAAATA TCCAATTGAT 
CTTGCTGGTC TTCTTCAGTA TGTGGCTAAT CAGCTAAAGG CAGGCAAAAG TTTTGACCTG 
CTTATACTGA AAGAAGTGGT ACAAAAAATG GCAGGAATAG AAATTACAGA AGAAATGACA 
ATGGAGCAAC TAGAAGCCAT GACTGGTGGA GAGCAACTAA AAGCTGAGGG TGGTTATTTT 
GGCCAGATAA GAAACACGAA AAAATCCTCC CAGAGATTAA AGGATGCACT ATTAGACCAT 
GATCTTGCTC TTCCTCTCTG CTTGCTTATG GCTCAGCAGA GGAATGGGGT AATCTTTCAG 
GAAGGTGGAG AGAAACATTT GAAACTTGTG GGAAAACTCT ATGATCAGTG TCATGATACC 
CTGGTACAGT TTGGTGGGTT TTTAGCATCT AATCTAAGCA CAGAAGATTA TATAAAGCGA 
GTGCCTTCAA TTGATGTGCT CTGTAATGAA TTTCACACAC CTCATGATGC AGCATTTTTC 
CTGTCTAGGC CAATGTATGC ACACCATATT TCGTCAAAGT ATGACGAACT TAAAAAATCA 
GAGAAGGGAA GTAAACAGCA GCATAAAGTT CATAAGTACA TCACATCATG TGAAATGGTG 
ATGGCTCCTG TTCATGAAGC AGTGGTTTCC TTACACGTTT CCAAAGTCTG GGATGACATC 
AGTCCTCAAT TCTATGCCAC ATTCTGGTCA TTGACAATGT ATGACCTTGC AGTTCCACAT 
ACCAGCTATG AACGGGAAGT CAATAAACTT AAAATCCAGA TGAAAGCAAT TGATGACAAT 
CAGGAAATGC CTCCAAATAA AAAGAAAAAA GAGAAGGAGC GATGTACTGC CCTTCAGGAC 
AAGCTTCTCG AAGAAGAAAA GAAACAGATG GAACATGTAC AGCGAGTTCT GCAGAGACTG 
AAACTGGAAA AGGACAACTG GCTTTTAGCA AAATCTACCA AAAATGAGAC CATCACAAAA 
TTTCTACAGC TGTGTATATT TCCTCGATGT ATTTTTTCAG CAATTGATGC TGTTTACTGT 
GCTCGTTTTG TTGAATTGGT ACATCAACAG AAAACTCCAA ATTTTTCCAC ACTTCTTTGC 
TATGATCGAG TTTTCTCTGA TATAATTTAC ACAGTTGCAA GCTGTACTGA AAATGAAGCT 
AGTCGATATG GGAGATTCCT TTGCTGCATG TTAGAGACTG TGACCAGGTG GCACAGTGAT 
AGAGCCACAT ATGAAAAGGA ATGTGGAAAT TATCCAGGAT TCCTTACTAT ATTACGAGCA 
ACTGGATTTG ATGGTGGAAA TAAAGCTGAT CAATTAGACT ATGAAAATTT TCGACATGTT 
GTACACAAAT GGCATTACAA ACTAACCAAG GCCTCGGTAC ATTGCCTTGA AACAGGCGAA 
TATACTCACA TCAGGAATAT CTTGATTGTG CTAACAAAAA TACTTCCTTG GTACCCAAAA 
GTTTTGAATC TGGGTCAGGC ATTGGAAAGA AGAGTGCATA AAATCTGCCA AGAGGAAAAA 
GAGAAGAGGC CAGATCTATA TGCATTGGCT ATGGGCTACT CTGGGCAGTT GAAAAGTAGA 
AAGTCATACA TGATACCTGA AAATGAGTTT CATCACAAAG ACCCCCCTCC GAGGAATGCA 
GTTGCCAGTG TACAAAATGG GCCTGGTGGT GGGCCTTCTT CATCGTCGAT CGGAAGTGTA 
TCTAAGTCAG ACGAAAGCAG TACCGAGGAC ACTGATAAAT CAAGGGAGAG ATCTCAGTGT 
GGTGTGAAAG CTGTTAATAA AGCTTCTAGT GCCACACCAA AAGGGAATTC AAGTAATGGA 
AATAGTGGCT CTAACAGCAG CAAAACTGTT AAAGAAAATG ACAAAGAAAA AGGAAAAGAG 
AAAGAAAAAG AGAAAAAAGA AAAGACTCCA GCTACTACTC CAGAGGCCCG AGTACTTGGT 
AAAGATGGTA AAGAAAAACC AAAGGAAGAA CGGCCAAATA AAGATGAAAA AGCAAGAGAG 
ACAAAGGAAA GAACACCTAA ATCTGACAAA GAGAAAGAAA AATTCAAGAA GGAAGAAAAA 
GCTAAAGATG AGAAATTCAA GACCACTGTC CCGAATGTAG AATCAAAGTC AACTCAAGAA 
AAGGAAAGAG AGAAGGAGCC GTCCAGAGAA CGAGATATAG CAAAGGAAAT GAAATCAAAG 
GAAAATGTTA AAGGAGGAGA GAAAACACCA GTTTCTGGGT CCTTGAAATC ACCTGTTCCC 
CGATCAGATA TTACAGAGCC TGAAAGGGAA CAGAAACGTC GCAAAATTGA TACCCATCCT 
TCTCCATCAC ATTCCTCAAC AGTAAAGATG GTTTATTTCC AGGTTACAGC CATACTTCCC 
AAAGTTCCTC TGGGTTCTGA GAACTATGCC AGCTCACCTG TCATCTCCAT TCATTTTCTA 
CAGGACAGTC TCATCGAACT CAAGGAGTCT TCAGCAAAGC TCTACATTAA TCATACTCCT 
CCACCACTGT CCAAGAGTAA GGAGAGAGAA ATGGACAAGA AAGATTTGGA CAAGTCAAGG 
GAAAGATCCA GAGAAAGAGA AAAAAAAGAT GAAAAGGACA GGAAAGAGCG GAAAAGGGAT 
CACTCAAACA ATGACCGAGA AGTGCCACCG GACTTAACCA AGAGGCGGAA AGAGGAAAAT 
GGAACAATGG GGGTTTCAAA GCACAAAAGT GAAAGTCCAT GTGAGTCTCC TTATCCAAAT 
GAGAAAGACA AGGAAAAAAA TAAGTCAAAA TCTTCAGGCA AAGAAAAAGG CGGCGACTCA 
TTTAAATCTG AAAAGATGGA TAAAATCTCC TCTGGTGGCA AAAAGGAGTC CAGGCATGAT 
AAAGAAAAGA TAGAAAAGAA AGAGAAACGG GACAGTTCAG GAGGAAAGGA AGAGAAGAAA 
CATCATAAGT CCTCGGACAA GCACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025132242.1
CDS	86..4975
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYVCFEILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKARETKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKMVYFQVTAILPKVPLGSENYASSPVISIHFLQDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKHHKSSDKHR

Target ORF information:

RefSeq Version	XM_025276457.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025276457.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATGTAT GTTTTGAGAT CTTGTCACAA ATACAGAAGT ATGATAACTT GATAACACCT 
GTAGTAGATT CATTGAAATA CCTCACTTCG TTGAATTATG ACGTCTTGGC CTATTGTATC 
ATTGAAGCTT TAGCTAATCC AGAAAAGGAG AAAATGAAAC ATGATGACAC AACCATCTCA 
AGTTGGCTTC AGAGTCTGGC TAGTTTCTGT GGTGCAGTTT TTCGTAAATA TCCAATTGAT 
CTTGCTGGTC TTCTTCAGTA TGTGGCTAAT CAGCTAAAGG CAGGCAAAAG TTTTGACCTG 
CTTATACTGA AAGAAGTGGT ACAAAAAATG GCAGGAATAG AAATTACAGA AGAAATGACA 
ATGGAGCAAC TAGAAGCCAT GACTGGTGGA GAGCAACTAA AAGCTGAGGG TGGTTATTTT 
GGCCAGATAA GAAACACGAA AAAATCCTCC CAGAGATTAA AGGATGCACT ATTAGACCAT 
GATCTTGCTC TTCCTCTCTG CTTGCTTATG GCTCAGCAGA GGAATGGGGT AATCTTTCAG 
GAAGGTGGAG AGAAACATTT GAAACTTGTG GGAAAACTCT ATGATCAGTG TCATGATACC 
CTGGTACAGT TTGGTGGGTT TTTAGCATCT AATCTAAGCA CAGAAGATTA TATAAAGCGA 
GTGCCTTCAA TTGATGTGCT CTGTAATGAA TTTCACACAC CTCATGATGC AGCATTTTTC 
CTGTCTAGGC CAATGTATGC ACACCATATT TCGTCAAAGT ATGACGAACT TAAAAAATCA 
GAGAAGGGAA GTAAACAGCA GCATAAAGTT CATAAGTACA TCACATCATG TGAAATGGTG 
ATGGCTCCTG TTCATGAAGC AGTGGTTTCC TTACACGTTT CCAAAGTCTG GGATGACATC 
AGTCCTCAAT TCTATGCCAC ATTCTGGTCA TTGACAATGT ATGACCTTGC AGTTCCACAT 
ACCAGCTATG AACGGGAAGT CAATAAACTT AAAATCCAGA TGAAAGCAAT TGATGACAAT 
CAGGAAATGC CTCCAAATAA AAAGAAAAAA GAGAAGGAGC GATGTACTGC CCTTCAGGAC 
AAGCTTCTCG AAGAAGAAAA GAAACAGATG GAACATGTAC AGCGAGTTCT GCAGAGACTG 
AAACTGGAAA AGGACAACTG GCTTTTAGCA AAATCTACCA AAAATGAGAC CATCACAAAA 
TTTCTACAGC TGTGTATATT TCCTCGATGT ATTTTTTCAG CAATTGATGC TGTTTACTGT 
GCTCGTTTTG TTGAATTGGT ACATCAACAG AAAACTCCAA ATTTTTCCAC ACTTCTTTGC 
TATGATCGAG TTTTCTCTGA TATAATTTAC ACAGTTGCAA GCTGTACTGA AAATGAAGCT 
AGTCGATATG GGAGATTCCT TTGCTGCATG TTAGAGACTG TGACCAGGTG GCACAGTGAT 
AGAGCCACAT ATGAAAAGGA ATGTGGAAAT TATCCAGGAT TCCTTACTAT ATTACGAGCA 
ACTGGATTTG ATGGTGGAAA TAAAGCTGAT CAATTAGACT ATGAAAATTT TCGACATGTT 
GTACACAAAT GGCATTACAA ACTAACCAAG GCCTCGGTAC ATTGCCTTGA AACAGGCGAA 
TATACTCACA TCAGGAATAT CTTGATTGTG CTAACAAAAA TACTTCCTTG GTACCCAAAA 
GTTTTGAATC TGGGTCAGGC ATTGGAAAGA AGAGTGCATA AAATCTGCCA AGAGGAAAAA 
GAGAAGAGGC CAGATCTATA TGCATTGGCT ATGGGCTACT CTGGGCAGTT GAAAAGTAGA 
AAGTCATACA TGATACCTGA AAATGAGTTT CATCACAAAG ACCCCCCTCC GAGGAATGCA 
GTTGCCAGTG TACAAAATGG GCCTGGTGGT GGGCCTTCTT CATCGTCGAT CGGAAGTGTA 
TCTAAGTCAG ACGAAAGCAG TACCGAGGAC ACTGATAAAT CAAGGGAGAG ATCTCAGTGT 
GGTGTGAAAG CTGTTAATAA AGCTTCTAGT GCCACACCAA AAGGGAATTC AAGTAATGGA 
AATAGTGGCT CTAACAGCAG CAAAACTGTT AAAGAAAATG ACAAAGAAAA AGGAAAAGAG 
AAAGAAAAAG AGAAAAAAGA AAAGACTCCA GCTACTACTC CAGAGGCCCG AGTACTTGGT 
AAAGATGGTA AAGAAAAACC AAAGGAAGAA CGGCCAAATA AAGATGAAAA AGCAAGAGAG 
ACAAAGGAAA GAACACCTAA ATCTGACAAA GAGAAAGAAA AATTCAAGAA GGAAGAAAAA 
GCTAAAGATG AGAAATTCAA GACCACTGTC CCGAATGTAG AATCAAAGTC AACTCAAGAA 
AAGGAAAGAG AGAAGGAGCC GTCCAGAGAA CGAGATATAG CAAAGGAAAT GAAATCAAAG 
GAAAATGTTA AAGGAGGAGA GAAAACACCA GTTTCTGGGT CCTTGAAATC ACCTGTTCCC 
CGATCAGATA TTACAGAGCC TGAAAGGGAA CAGAAACGTC GCAAAATTGA TACCCATCCT 
TCTCCATCAC ATTCCTCAAC AGTAAAGATG GTTTATTTCC AGGTTACAGC CATACTTCCC 
AAAGTTCCTC TGGGTTCTGA GAACTATGCC AGCTCACCTG TCATCTCCAT TCATTTTCTA 
CAGGACAGTC TCATCGAACT CAAGGAGTCT TCAGCAAAGC TCTACATTAA TCATACTCCT 
CCACCACTGT CCAAGAGTAA GGAGAGAGAA ATGGACAAGA AAGATTTGGA CAAGTCAAGG 
GAAAGATCCA GAGAAAGAGA AAAAAAAGAT GAAAAGGACA GGAAAGAGCG GAAAAGGGAT 
CACTCAAACA ATGACCGAGA AGTGCCACCG GACTTAACCA AGAGGCGGAA AGAGGAAAAT 
GGAACAATGG GGGTTTCAAA GCACAAAAGT GAAAGTCCAT GTGAGTCTCC TTATCCAAAT 
GAGAAAGACA AGGAAAAAAA TAAGTCAAAA TCTTCAGGCA AAGAAAAAGG CGGCGACTCA 
TTTAAATCTG AAAAGATGGA TAAAATCTCC TCTGGTGGCA AAAAGGAGTC CAGGCATGAT 
AAAGAAAAGA TAGAAAAGAA AGAGAAACGG GACAGTTCAG GAGGAAAGGA AGAGAAGAAA 
CATCATAAGT CCTCGGACAA GCACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_025276458.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025132243.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X2, mRNA.

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CloneID	OBu73753
Clone ID Related Accession (Same CDS sequence)	XM_025276457.1 , XM_025276458.1
Accession Version	XM_025276458.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATGTAT GTTTTGAGAT CTTGTCACAA ATACAGAAGT ATGATAACTT GATAACACCT 
GTAGTAGATT CATTGAAATA CCTCACTTCG TTGAATTATG ACGTCTTGGC CTATTGTATC 
ATTGAAGCTT TAGCTAATCC AGAAAAGGAG AAAATGAAAC ATGATGACAC AACCATCTCA 
AGTTGGCTTC AGAGTCTGGC TAGTTTCTGT GGTGCAGTTT TTCGTAAATA TCCAATTGAT 
CTTGCTGGTC TTCTTCAGTA TGTGGCTAAT CAGCTAAAGG CAGGCAAAAG TTTTGACCTG 
CTTATACTGA AAGAAGTGGT ACAAAAAATG GCAGGAATAG AAATTACAGA AGAAATGACA 
ATGGAGCAAC TAGAAGCCAT GACTGGTGGA GAGCAACTAA AAGCTGAGGG TGGTTATTTT 
GGCCAGATAA GAAACACGAA AAAATCCTCC CAGAGATTAA AGGATGCACT ATTAGACCAT 
GATCTTGCTC TTCCTCTCTG CTTGCTTATG GCTCAGCAGA GGAATGGGGT AATCTTTCAG 
GAAGGTGGAG AGAAACATTT GAAACTTGTG GGAAAACTCT ATGATCAGTG TCATGATACC 
CTGGTACAGT TTGGTGGGTT TTTAGCATCT AATCTAAGCA CAGAAGATTA TATAAAGCGA 
GTGCCTTCAA TTGATGTGCT CTGTAATGAA TTTCACACAC CTCATGATGC AGCATTTTTC 
CTGTCTAGGC CAATGTATGC ACACCATATT TCGTCAAAGT ATGACGAACT TAAAAAATCA 
GAGAAGGGAA GTAAACAGCA GCATAAAGTT CATAAGTACA TCACATCATG TGAAATGGTG 
ATGGCTCCTG TTCATGAAGC AGTGGTTTCC TTACACGTTT CCAAAGTCTG GGATGACATC 
AGTCCTCAAT TCTATGCCAC ATTCTGGTCA TTGACAATGT ATGACCTTGC AGTTCCACAT 
ACCAGCTATG AACGGGAAGT CAATAAACTT AAAATCCAGA TGAAAGCAAT TGATGACAAT 
CAGGAAATGC CTCCAAATAA AAAGAAAAAA GAGAAGGAGC GATGTACTGC CCTTCAGGAC 
AAGCTTCTCG AAGAAGAAAA GAAACAGATG GAACATGTAC AGCGAGTTCT GCAGAGACTG 
AAACTGGAAA AGGACAACTG GCTTTTAGCA AAATCTACCA AAAATGAGAC CATCACAAAA 
TTTCTACAGC TGTGTATATT TCCTCGATGT ATTTTTTCAG CAATTGATGC TGTTTACTGT 
GCTCGTTTTG TTGAATTGGT ACATCAACAG AAAACTCCAA ATTTTTCCAC ACTTCTTTGC 
TATGATCGAG TTTTCTCTGA TATAATTTAC ACAGTTGCAA GCTGTACTGA AAATGAAGCT 
AGTCGATATG GGAGATTCCT TTGCTGCATG TTAGAGACTG TGACCAGGTG GCACAGTGAT 
AGAGCCACAT ATGAAAAGGA ATGTGGAAAT TATCCAGGAT TCCTTACTAT ATTACGAGCA 
ACTGGATTTG ATGGTGGAAA TAAAGCTGAT CAATTAGACT ATGAAAATTT TCGACATGTT 
GTACACAAAT GGCATTACAA ACTAACCAAG GCCTCGGTAC ATTGCCTTGA AACAGGCGAA 
TATACTCACA TCAGGAATAT CTTGATTGTG CTAACAAAAA TACTTCCTTG GTACCCAAAA 
GTTTTGAATC TGGGTCAGGC ATTGGAAAGA AGAGTGCATA AAATCTGCCA AGAGGAAAAA 
GAGAAGAGGC CAGATCTATA TGCATTGGCT ATGGGCTACT CTGGGCAGTT GAAAAGTAGA 
AAGTCATACA TGATACCTGA AAATGAGTTT CATCACAAAG ACCCCCCTCC GAGGAATGCA 
GTTGCCAGTG TACAAAATGG GCCTGGTGGT GGGCCTTCTT CATCGTCGAT CGGAAGTGTA 
TCTAAGTCAG ACGAAAGCAG TACCGAGGAC ACTGATAAAT CAAGGGAGAG ATCTCAGTGT 
GGTGTGAAAG CTGTTAATAA AGCTTCTAGT GCCACACCAA AAGGGAATTC AAGTAATGGA 
AATAGTGGCT CTAACAGCAG CAAAACTGTT AAAGAAAATG ACAAAGAAAA AGGAAAAGAG 
AAAGAAAAAG AGAAAAAAGA AAAGACTCCA GCTACTACTC CAGAGGCCCG AGTACTTGGT 
AAAGATGGTA AAGAAAAACC AAAGGAAGAA CGGCCAAATA AAGATGAAAA AGCAAGAGAG 
ACAAAGGAAA GAACACCTAA ATCTGACAAA GAGAAAGAAA AATTCAAGAA GGAAGAAAAA 
GCTAAAGATG AGAAATTCAA GACCACTGTC CCGAATGTAG AATCAAAGTC AACTCAAGAA 
AAGGAAAGAG AGAAGGAGCC GTCCAGAGAA CGAGATATAG CAAAGGAAAT GAAATCAAAG 
GAAAATGTTA AAGGAGGAGA GAAAACACCA GTTTCTGGGT CCTTGAAATC ACCTGTTCCC 
CGATCAGATA TTACAGAGCC TGAAAGGGAA CAGAAACGTC GCAAAATTGA TACCCATCCT 
TCTCCATCAC ATTCCTCAAC AGTAAAGATG GTTTATTTCC AGGTTACAGC CATACTTCCC 
AAAGTTCCTC TGGGTTCTGA GAACTATGCC AGCTCACCTG TCATCTCCAT TCATTTTCTA 
CAGGACAGTC TCATCGAACT CAAGGAGTCT TCAGCAAAGC TCTACATTAA TCATACTCCT 
CCACCACTGT CCAAGAGTAA GGAGAGAGAA ATGGACAAGA AAGATTTGGA CAAGTCAAGG 
GAAAGATCCA GAGAAAGAGA AAAAAAAGAT GAAAAGGACA GGAAAGAGCG GAAAAGGGAT 
CACTCAAACA ATGACCGAGA AGTGCCACCG GACTTAACCA AGAGGCGGAA AGAGGAAAAT 
GGAACAATGG GGGTTTCAAA GCACAAAAGT GAAAGTCCAT GTGAGTCTCC TTATCCAAAT 
GAGAAAGACA AGGAAAAAAA TAAGTCAAAA TCTTCAGGCA AAGAAAAAGG CGGCGACTCA 
TTTAAATCTG AAAAGATGGA TAAAATCTCC TCTGGTGGCA AAAAGGAGTC CAGGCATGAT 
AAAGAAAAGA TAGAAAAGAA AGAGAAACGG GACAGTTCAG GAGGAAAGGA AGAGAAGAAA 
CATCATAAGT CCTCGGACAA GCACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025132243.1
CDS	86..4975
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYVCFEILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKARETKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKMVYFQVTAILPKVPLGSENYASSPVISIHFLQDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKHHKSSDKHR

Target ORF information:

RefSeq Version	XM_025276458.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025276458.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATGTAT GTTTTGAGAT CTTGTCACAA ATACAGAAGT ATGATAACTT GATAACACCT 
GTAGTAGATT CATTGAAATA CCTCACTTCG TTGAATTATG ACGTCTTGGC CTATTGTATC 
ATTGAAGCTT TAGCTAATCC AGAAAAGGAG AAAATGAAAC ATGATGACAC AACCATCTCA 
AGTTGGCTTC AGAGTCTGGC TAGTTTCTGT GGTGCAGTTT TTCGTAAATA TCCAATTGAT 
CTTGCTGGTC TTCTTCAGTA TGTGGCTAAT CAGCTAAAGG CAGGCAAAAG TTTTGACCTG 
CTTATACTGA AAGAAGTGGT ACAAAAAATG GCAGGAATAG AAATTACAGA AGAAATGACA 
ATGGAGCAAC TAGAAGCCAT GACTGGTGGA GAGCAACTAA AAGCTGAGGG TGGTTATTTT 
GGCCAGATAA GAAACACGAA AAAATCCTCC CAGAGATTAA AGGATGCACT ATTAGACCAT 
GATCTTGCTC TTCCTCTCTG CTTGCTTATG GCTCAGCAGA GGAATGGGGT AATCTTTCAG 
GAAGGTGGAG AGAAACATTT GAAACTTGTG GGAAAACTCT ATGATCAGTG TCATGATACC 
CTGGTACAGT TTGGTGGGTT TTTAGCATCT AATCTAAGCA CAGAAGATTA TATAAAGCGA 
GTGCCTTCAA TTGATGTGCT CTGTAATGAA TTTCACACAC CTCATGATGC AGCATTTTTC 
CTGTCTAGGC CAATGTATGC ACACCATATT TCGTCAAAGT ATGACGAACT TAAAAAATCA 
GAGAAGGGAA GTAAACAGCA GCATAAAGTT CATAAGTACA TCACATCATG TGAAATGGTG 
ATGGCTCCTG TTCATGAAGC AGTGGTTTCC TTACACGTTT CCAAAGTCTG GGATGACATC 
AGTCCTCAAT TCTATGCCAC ATTCTGGTCA TTGACAATGT ATGACCTTGC AGTTCCACAT 
ACCAGCTATG AACGGGAAGT CAATAAACTT AAAATCCAGA TGAAAGCAAT TGATGACAAT 
CAGGAAATGC CTCCAAATAA AAAGAAAAAA GAGAAGGAGC GATGTACTGC CCTTCAGGAC 
AAGCTTCTCG AAGAAGAAAA GAAACAGATG GAACATGTAC AGCGAGTTCT GCAGAGACTG 
AAACTGGAAA AGGACAACTG GCTTTTAGCA AAATCTACCA AAAATGAGAC CATCACAAAA 
TTTCTACAGC TGTGTATATT TCCTCGATGT ATTTTTTCAG CAATTGATGC TGTTTACTGT 
GCTCGTTTTG TTGAATTGGT ACATCAACAG AAAACTCCAA ATTTTTCCAC ACTTCTTTGC 
TATGATCGAG TTTTCTCTGA TATAATTTAC ACAGTTGCAA GCTGTACTGA AAATGAAGCT 
AGTCGATATG GGAGATTCCT TTGCTGCATG TTAGAGACTG TGACCAGGTG GCACAGTGAT 
AGAGCCACAT ATGAAAAGGA ATGTGGAAAT TATCCAGGAT TCCTTACTAT ATTACGAGCA 
ACTGGATTTG ATGGTGGAAA TAAAGCTGAT CAATTAGACT ATGAAAATTT TCGACATGTT 
GTACACAAAT GGCATTACAA ACTAACCAAG GCCTCGGTAC ATTGCCTTGA AACAGGCGAA 
TATACTCACA TCAGGAATAT CTTGATTGTG CTAACAAAAA TACTTCCTTG GTACCCAAAA 
GTTTTGAATC TGGGTCAGGC ATTGGAAAGA AGAGTGCATA AAATCTGCCA AGAGGAAAAA 
GAGAAGAGGC CAGATCTATA TGCATTGGCT ATGGGCTACT CTGGGCAGTT GAAAAGTAGA 
AAGTCATACA TGATACCTGA AAATGAGTTT CATCACAAAG ACCCCCCTCC GAGGAATGCA 
GTTGCCAGTG TACAAAATGG GCCTGGTGGT GGGCCTTCTT CATCGTCGAT CGGAAGTGTA 
TCTAAGTCAG ACGAAAGCAG TACCGAGGAC ACTGATAAAT CAAGGGAGAG ATCTCAGTGT 
GGTGTGAAAG CTGTTAATAA AGCTTCTAGT GCCACACCAA AAGGGAATTC AAGTAATGGA 
AATAGTGGCT CTAACAGCAG CAAAACTGTT AAAGAAAATG ACAAAGAAAA AGGAAAAGAG 
AAAGAAAAAG AGAAAAAAGA AAAGACTCCA GCTACTACTC CAGAGGCCCG AGTACTTGGT 
AAAGATGGTA AAGAAAAACC AAAGGAAGAA CGGCCAAATA AAGATGAAAA AGCAAGAGAG 
ACAAAGGAAA GAACACCTAA ATCTGACAAA GAGAAAGAAA AATTCAAGAA GGAAGAAAAA 
GCTAAAGATG AGAAATTCAA GACCACTGTC CCGAATGTAG AATCAAAGTC AACTCAAGAA 
AAGGAAAGAG AGAAGGAGCC GTCCAGAGAA CGAGATATAG CAAAGGAAAT GAAATCAAAG 
GAAAATGTTA AAGGAGGAGA GAAAACACCA GTTTCTGGGT CCTTGAAATC ACCTGTTCCC 
CGATCAGATA TTACAGAGCC TGAAAGGGAA CAGAAACGTC GCAAAATTGA TACCCATCCT 
TCTCCATCAC ATTCCTCAAC AGTAAAGATG GTTTATTTCC AGGTTACAGC CATACTTCCC 
AAAGTTCCTC TGGGTTCTGA GAACTATGCC AGCTCACCTG TCATCTCCAT TCATTTTCTA 
CAGGACAGTC TCATCGAACT CAAGGAGTCT TCAGCAAAGC TCTACATTAA TCATACTCCT 
CCACCACTGT CCAAGAGTAA GGAGAGAGAA ATGGACAAGA AAGATTTGGA CAAGTCAAGG 
GAAAGATCCA GAGAAAGAGA AAAAAAAGAT GAAAAGGACA GGAAAGAGCG GAAAAGGGAT 
CACTCAAACA ATGACCGAGA AGTGCCACCG GACTTAACCA AGAGGCGGAA AGAGGAAAAT 
GGAACAATGG GGGTTTCAAA GCACAAAAGT GAAAGTCCAT GTGAGTCTCC TTATCCAAAT 
GAGAAAGACA AGGAAAAAAA TAAGTCAAAA TCTTCAGGCA AAGAAAAAGG CGGCGACTCA 
TTTAAATCTG AAAAGATGGA TAAAATCTCC TCTGGTGGCA AAAAGGAGTC CAGGCATGAT 
AAAGAAAAGA TAGAAAAGAA AGAGAAACGG GACAGTTCAG GAGGAAAGGA AGAGAAGAAA 
CATCATAAGT CCTCGGACAA GCACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_025276459.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025132244.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X3, mRNA.

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CloneID	OBu73754
Clone ID Related Accession (Same CDS sequence)	XM_025276459.1
Accession Version	XM_025276459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATGTAT GTTTTGAGAT CTTGTCACAA ATACAGAAGT ATGATAACTT GATAACACCT 
GTAGTAGATT CATTGAAATA CCTCACTTCG TTGAATTATG ACGTCTTGGC CTATTGTATC 
ATTGAAGCTT TAGCTAATCC AGAAAAGGAG AAAATGAAAC ATGATGACAC AACCATCTCA 
AGTTGGCTTC AGAGTCTGGC TAGTTTCTGT GGTGCAGTTT TTCGTAAATA TCCAATTGAT 
CTTGCTGGTC TTCTTCAGTA TGTGGCTAAT CAGCTAAAGG CAGGCAAAAG TTTTGACCTG 
CTTATACTGA AAGAAGTGGT ACAAAAAATG GCAGGAATAG AAATTACAGA AGAAATGACA 
ATGGAGCAAC TAGAAGCCAT GACTGGTGGA GAGCAACTAA AAGCTGAGGG TGGTTATTTT 
GGCCAGATAA GAAACACGAA AAAATCCTCC CAGAGATTAA AGGATGCACT ATTAGACCAT 
GATCTTGCTC TTCCTCTCTG CTTGCTTATG GCTCAGCAGA GGAATGGGGT AATCTTTCAG 
GAAGGTGGAG AGAAACATTT GAAACTTGTG GGAAAACTCT ATGATCAGTG TCATGATACC 
CTGGTACAGT TTGGTGGGTT TTTAGCATCT AATCTAAGCA CAGAAGATTA TATAAAGCGA 
GTGCCTTCAA TTGATGTGCT CTGTAATGAA TTTCACACAC CTCATGATGC AGCATTTTTC 
CTGTCTAGGC CAATGTATGC ACACCATATT TCGTCAAAGT ATGACGAACT TAAAAAATCA 
GAGAAGGGAA GTAAACAGCA GCATAAAGTT CATAAGTACA TCACATCATG TGAAATGGTG 
ATGGCTCCTG TTCATGAAGC AGTGGTTTCC TTACACGTTT CCAAAGTCTG GGATGACATC 
AGTCCTCAAT TCTATGCCAC ATTCTGGTCA TTGACAATGT ATGACCTTGC AGTTCCACAT 
ACCAGCTATG AACGGGAAGT CAATAAACTT AAAATCCAGA TGAAAGCAAT TGATGACAAT 
CAGGAAATGC CTCCAAATAA AAAGAAAAAA GAGAAGGAGC GATGTACTGC CCTTCAGGAC 
AAGCTTCTCG AAGAAGAAAA GAAACAGATG GAACATGTAC AGCGAGTTCT GCAGAGACTG 
AAACTGGAAA AGGACAACTG GCTTTTAGCA AAATCTACCA AAAATGAGAC CATCACAAAA 
TTTCTACAGC TGTGTATATT TCCTCGATGT ATTTTTTCAG CAATTGATGC TGTTTACTGT 
GCTCGTTTTG TTGAATTGGT ACATCAACAG AAAACTCCAA ATTTTTCCAC ACTTCTTTGC 
TATGATCGAG TTTTCTCTGA TATAATTTAC ACAGTTGCAA GCTGTACTGA AAATGAAGCT 
AGTCGATATG GGAGATTCCT TTGCTGCATG TTAGAGACTG TGACCAGGTG GCACAGTGAT 
AGAGCCACAT ATGAAAAGGA ATGTGGAAAT TATCCAGGAT TCCTTACTAT ATTACGAGCA 
ACTGGATTTG ATGGTGGAAA TAAAGCTGAT CAATTAGACT ATGAAAATTT TCGACATGTT 
GTACACAAAT GGCATTACAA ACTAACCAAG GCCTCGGTAC ATTGCCTTGA AACAGGCGAA 
TATACTCACA TCAGGAATAT CTTGATTGTG CTAACAAAAA TACTTCCTTG GTACCCAAAA 
GTTTTGAATC TGGGTCAGGC ATTGGAAAGA AGAGTGCATA AAATCTGCCA AGAGGAAAAA 
GAGAAGAGGC CAGATCTATA TGCATTGGCT ATGGGCTACT CTGGGCAGTT GAAAAGTAGA 
AAGTCATACA TGATACCTGA AAATGAGTTT CATCACAAAG ACCCCCCTCC GAGGAATGCA 
GTTGCCAGTG TACAAAATGG GCCTGGTGGT GGGCCTTCTT CATCGTCGAT CGGAAGTGTA 
TCTAAGTCAG ACGAAAGCAG TACCGAGGAC ACTGATAAAT CAAGGGAGAG ATCTCAGTGT 
GGTGTGAAAG CTGTTAATAA AGCTTCTAGT GCCACACCAA AAGGGAATTC AAGTAATGGA 
AATAGTGGCT CTAACAGCAA AACTGTTAAA GAAAATGACA AAGAAAAAGG AAAAGAGAAA 
GAAAAAGAGA AAAAAGAAAA GACTCCAGCT ACTACTCCAG AGGCCCGAGT ACTTGGTAAA 
GATGGTAAAG AAAAACCAAA GGAAGAACGG CCAAATAAAG ATGAAAAAGC AAGAGAGACA 
AAGGAAAGAA CACCTAAATC TGACAAAGAG AAAGAAAAAT TCAAGAAGGA AGAAAAAGCT 
AAAGATGAGA AATTCAAGAC CACTGTCCCG AATGTAGAAT CAAAGTCAAC TCAAGAAAAG 
GAAAGAGAGA AGGAGCCGTC CAGAGAACGA GATATAGCAA AGGAAATGAA ATCAAAGGAA 
AATGTTAAAG GAGGAGAGAA AACACCAGTT TCTGGGTCCT TGAAATCACC TGTTCCCCGA 
TCAGATATTA CAGAGCCTGA AAGGGAACAG AAACGTCGCA AAATTGATAC CCATCCTTCT 
CCATCACATT CCTCAACAGT AAAGATGGTT TATTTCCAGG TTACAGCCAT ACTTCCCAAA 
GTTCCTCTGG GTTCTGAGAA CTATGCCAGC TCACCTGTCA TCTCCATTCA TTTTCTACAG 
GACAGTCTCA TCGAACTCAA GGAGTCTTCA GCAAAGCTCT ACATTAATCA TACTCCTCCA 
CCACTGTCCA AGAGTAAGGA GAGAGAAATG GACAAGAAAG ATTTGGACAA GTCAAGGGAA 
AGATCCAGAG AAAGAGAAAA AAAAGATGAA AAGGACAGGA AAGAGCGGAA AAGGGATCAC 
TCAAACAATG ACCGAGAAGT GCCACCGGAC TTAACCAAGA GGCGGAAAGA GGAAAATGGA 
ACAATGGGGG TTTCAAAGCA CAAAAGTGAA AGTCCATGTG AGTCTCCTTA TCCAAATGAG 
AAAGACAAGG AAAAAAATAA GTCAAAATCT TCAGGCAAAG AAAAAGGCGG CGACTCATTT 
AAATCTGAAA AGATGGATAA AATCTCCTCT GGTGGCAAAA AGGAGTCCAG GCATGATAAA 
GAAAAGATAG AAAAGAAAGA GAAACGGGAC AGTTCAGGAG GAAAGGAAGA GAAGAAACAT 
CATAAGTCCT CGGACAAGCA CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025132244.1
CDS	86..4972
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYVCFEILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKARETKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKMVYFQVTAILPKVPLGSENYASSPVISIHFLQDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKHHKSSDKHR

Target ORF information:

RefSeq Version	XM_025276459.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025276459.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATGTAT GTTTTGAGAT CTTGTCACAA ATACAGAAGT ATGATAACTT GATAACACCT 
GTAGTAGATT CATTGAAATA CCTCACTTCG TTGAATTATG ACGTCTTGGC CTATTGTATC 
ATTGAAGCTT TAGCTAATCC AGAAAAGGAG AAAATGAAAC ATGATGACAC AACCATCTCA 
AGTTGGCTTC AGAGTCTGGC TAGTTTCTGT GGTGCAGTTT TTCGTAAATA TCCAATTGAT 
CTTGCTGGTC TTCTTCAGTA TGTGGCTAAT CAGCTAAAGG CAGGCAAAAG TTTTGACCTG 
CTTATACTGA AAGAAGTGGT ACAAAAAATG GCAGGAATAG AAATTACAGA AGAAATGACA 
ATGGAGCAAC TAGAAGCCAT GACTGGTGGA GAGCAACTAA AAGCTGAGGG TGGTTATTTT 
GGCCAGATAA GAAACACGAA AAAATCCTCC CAGAGATTAA AGGATGCACT ATTAGACCAT 
GATCTTGCTC TTCCTCTCTG CTTGCTTATG GCTCAGCAGA GGAATGGGGT AATCTTTCAG 
GAAGGTGGAG AGAAACATTT GAAACTTGTG GGAAAACTCT ATGATCAGTG TCATGATACC 
CTGGTACAGT TTGGTGGGTT TTTAGCATCT AATCTAAGCA CAGAAGATTA TATAAAGCGA 
GTGCCTTCAA TTGATGTGCT CTGTAATGAA TTTCACACAC CTCATGATGC AGCATTTTTC 
CTGTCTAGGC CAATGTATGC ACACCATATT TCGTCAAAGT ATGACGAACT TAAAAAATCA 
GAGAAGGGAA GTAAACAGCA GCATAAAGTT CATAAGTACA TCACATCATG TGAAATGGTG 
ATGGCTCCTG TTCATGAAGC AGTGGTTTCC TTACACGTTT CCAAAGTCTG GGATGACATC 
AGTCCTCAAT TCTATGCCAC ATTCTGGTCA TTGACAATGT ATGACCTTGC AGTTCCACAT 
ACCAGCTATG AACGGGAAGT CAATAAACTT AAAATCCAGA TGAAAGCAAT TGATGACAAT 
CAGGAAATGC CTCCAAATAA AAAGAAAAAA GAGAAGGAGC GATGTACTGC CCTTCAGGAC 
AAGCTTCTCG AAGAAGAAAA GAAACAGATG GAACATGTAC AGCGAGTTCT GCAGAGACTG 
AAACTGGAAA AGGACAACTG GCTTTTAGCA AAATCTACCA AAAATGAGAC CATCACAAAA 
TTTCTACAGC TGTGTATATT TCCTCGATGT ATTTTTTCAG CAATTGATGC TGTTTACTGT 
GCTCGTTTTG TTGAATTGGT ACATCAACAG AAAACTCCAA ATTTTTCCAC ACTTCTTTGC 
TATGATCGAG TTTTCTCTGA TATAATTTAC ACAGTTGCAA GCTGTACTGA AAATGAAGCT 
AGTCGATATG GGAGATTCCT TTGCTGCATG TTAGAGACTG TGACCAGGTG GCACAGTGAT 
AGAGCCACAT ATGAAAAGGA ATGTGGAAAT TATCCAGGAT TCCTTACTAT ATTACGAGCA 
ACTGGATTTG ATGGTGGAAA TAAAGCTGAT CAATTAGACT ATGAAAATTT TCGACATGTT 
GTACACAAAT GGCATTACAA ACTAACCAAG GCCTCGGTAC ATTGCCTTGA AACAGGCGAA 
TATACTCACA TCAGGAATAT CTTGATTGTG CTAACAAAAA TACTTCCTTG GTACCCAAAA 
GTTTTGAATC TGGGTCAGGC ATTGGAAAGA AGAGTGCATA AAATCTGCCA AGAGGAAAAA 
GAGAAGAGGC CAGATCTATA TGCATTGGCT ATGGGCTACT CTGGGCAGTT GAAAAGTAGA 
AAGTCATACA TGATACCTGA AAATGAGTTT CATCACAAAG ACCCCCCTCC GAGGAATGCA 
GTTGCCAGTG TACAAAATGG GCCTGGTGGT GGGCCTTCTT CATCGTCGAT CGGAAGTGTA 
TCTAAGTCAG ACGAAAGCAG TACCGAGGAC ACTGATAAAT CAAGGGAGAG ATCTCAGTGT 
GGTGTGAAAG CTGTTAATAA AGCTTCTAGT GCCACACCAA AAGGGAATTC AAGTAATGGA 
AATAGTGGCT CTAACAGCAA AACTGTTAAA GAAAATGACA AAGAAAAAGG AAAAGAGAAA 
GAAAAAGAGA AAAAAGAAAA GACTCCAGCT ACTACTCCAG AGGCCCGAGT ACTTGGTAAA 
GATGGTAAAG AAAAACCAAA GGAAGAACGG CCAAATAAAG ATGAAAAAGC AAGAGAGACA 
AAGGAAAGAA CACCTAAATC TGACAAAGAG AAAGAAAAAT TCAAGAAGGA AGAAAAAGCT 
AAAGATGAGA AATTCAAGAC CACTGTCCCG AATGTAGAAT CAAAGTCAAC TCAAGAAAAG 
GAAAGAGAGA AGGAGCCGTC CAGAGAACGA GATATAGCAA AGGAAATGAA ATCAAAGGAA 
AATGTTAAAG GAGGAGAGAA AACACCAGTT TCTGGGTCCT TGAAATCACC TGTTCCCCGA 
TCAGATATTA CAGAGCCTGA AAGGGAACAG AAACGTCGCA AAATTGATAC CCATCCTTCT 
CCATCACATT CCTCAACAGT AAAGATGGTT TATTTCCAGG TTACAGCCAT ACTTCCCAAA 
GTTCCTCTGG GTTCTGAGAA CTATGCCAGC TCACCTGTCA TCTCCATTCA TTTTCTACAG 
GACAGTCTCA TCGAACTCAA GGAGTCTTCA GCAAAGCTCT ACATTAATCA TACTCCTCCA 
CCACTGTCCA AGAGTAAGGA GAGAGAAATG GACAAGAAAG ATTTGGACAA GTCAAGGGAA 
AGATCCAGAG AAAGAGAAAA AAAAGATGAA AAGGACAGGA AAGAGCGGAA AAGGGATCAC 
TCAAACAATG ACCGAGAAGT GCCACCGGAC TTAACCAAGA GGCGGAAAGA GGAAAATGGA 
ACAATGGGGG TTTCAAAGCA CAAAAGTGAA AGTCCATGTG AGTCTCCTTA TCCAAATGAG 
AAAGACAAGG AAAAAAATAA GTCAAAATCT TCAGGCAAAG AAAAAGGCGG CGACTCATTT 
AAATCTGAAA AGATGGATAA AATCTCCTCT GGTGGCAAAA AGGAGTCCAG GCATGATAAA 
GAAAAGATAG AAAAGAAAGA GAAACGGGAC AGTTCAGGAG GAAAGGAAGA GAAGAAACAT 
CATAAGTCCT CGGACAAGCA CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_025276461.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025132246.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu73755
Clone ID Related Accession (Same CDS sequence)	XM_025276461.1 , XM_025276460.1
Accession Version	XM_025276461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATGTAT GTTTTGAGAT CTTGTCACAA ATACAGAAGT ATGATAACTT GATAACACCT 
GTAGTAGATT CATTGAAATA CCTCACTTCG TTGAATTATG ACGTCTTGGC CTATTGTATC 
ATTGAAGCTT TAGCTAATCC AGAAAAGGAG AAAATGAAAC ATGATGACAC AACCATCTCA 
AGTTGGCTTC AGAGTCTGGC TAGTTTCTGT GGTGCAGTTT TTCGTAAATA TCCAATTGAT 
CTTGCTGGTC TTCTTCAGTA TGTGGCTAAT CAGCTAAAGG CAGGCAAAAG TTTTGACCTG 
CTTATACTGA AAGAAGTGGT ACAAAAAATG GCAGGAATAG AAATTACAGA AGAAATGACA 
ATGGAGCAAC TAGAAGCCAT GACTGGTGGA GAGCAACTAA AAGCTGAGGG TGGTTATTTT 
GGCCAGATAA GAAACACGAA AAAATCCTCC CAGAGATTAA AGGATGCACT ATTAGACCAT 
GATCTTGCTC TTCCTCTCTG CTTGCTTATG GCTCAGCAGA GGAATGGGGT AATCTTTCAG 
GAAGGTGGAG AGAAACATTT GAAACTTGTG GGAAAACTCT ATGATCAGTG TCATGATACC 
CTGGTACAGT TTGGTGGGTT TTTAGCATCT AATCTAAGCA CAGAAGATTA TATAAAGCGA 
GTGCCTTCAA TTGATGTGCT CTGTAATGAA TTTCACACAC CTCATGATGC AGCATTTTTC 
CTGTCTAGGC CAATGTATGC ACACCATATT TCGTCAAAGT ATGACGAACT TAAAAAATCA 
GAGAAGGGAA GTAAACAGCA GCATAAAGTT CATAAGTACA TCACATCATG TGAAATGGTG 
ATGGCTCCTG TTCATGAAGC AGTGGTTTCC TTACACGTTT CCAAAGTCTG GGATGACATC 
AGTCCTCAAT TCTATGCCAC ATTCTGGTCA TTGACAATGT ATGACCTTGC AGTTCCACAT 
ACCAGCTATG AACGGGAAGT CAATAAACTT AAAATCCAGA TGAAAGCAAT TGATGACAAT 
CAGGAAATGC CTCCAAATAA AAAGAAAAAA GAGAAGGAGC GATGTACTGC CCTTCAGGAC 
AAGCTTCTCG AAGAAGAAAA GAAACAGATG GAACATGTAC AGCGAGTTCT GCAGAGACTG 
AAACTGGAAA AGGACAACTG GCTTTTAGCA AAATCTACCA AAAATGAGAC CATCACAAAA 
TTTCTACAGC TGTGTATATT TCCTCGATGT ATTTTTTCAG CAATTGATGC TGTTTACTGT 
GCTCGTTTTG TTGAATTGGT ACATCAACAG AAAACTCCAA ATTTTTCCAC ACTTCTTTGC 
TATGATCGAG TTTTCTCTGA TATAATTTAC ACAGTTGCAA GCTGTACTGA AAATGAAGCT 
AGTCGATATG GGAGATTCCT TTGCTGCATG TTAGAGACTG TGACCAGGTG GCACAGTGAT 
AGAGCCACAT ATGAAAAGGA ATGTGGAAAT TATCCAGGAT TCCTTACTAT ATTACGAGCA 
ACTGGATTTG ATGGTGGAAA TAAAGCTGAT CAATTAGACT ATGAAAATTT TCGACATGTT 
GTACACAAAT GGCATTACAA ACTAACCAAG GCCTCGGTAC ATTGCCTTGA AACAGGCGAA 
TATACTCACA TCAGGAATAT CTTGATTGTG CTAACAAAAA TACTTCCTTG GTACCCAAAA 
GTTTTGAATC TGGGTCAGGC ATTGGAAAGA AGAGTGCATA AAATCTGCCA AGAGGAAAAA 
GAGAAGAGGC CAGATCTATA TGCATTGGCT ATGGGCTACT CTGGGCAGTT GAAAAGTAGA 
AAGTCATACA TGATACCTGA AAATGAGTTT CATCACAAAG ACCCCCCTCC GAGGAATGCA 
GTTGCCAGTG TACAAAATGG GCCTGGTGGT GGGCCTTCTT CATCGTCGAT CGGAAGTGTA 
TCTAAGTCAG ACGAAAGCAG TACCGAGGAC ACTGATAAAT CAAGGGAGAG ATCTCAGTGT 
GGTGTGAAAG CTGTTAATAA AGCTTCTAGT GCCACACCAA AAGGGAATTC AAGTAATGGA 
AATAGTGGCT CTAACAGCAG CAAAACTGTT AAAGAAAATG ACAAAGAAAA AGGAAAAGAG 
AAAGAAAAAG AGAAAAAAGA AAAGACTCCA GCTACTACTC CAGAGGCCCG AGTACTTGGT 
AAAGATGGTA AAGAAAAACC AAAGGAAGAA CGGCCAAATA AAGATGAAAA AGCAAGAGAG 
ACAAAGGAAA GAACACCTAA ATCTGACAAA GAGAAAGAAA AATTCAAGAA GGAAGAAAAA 
GCTAAAGATG AGAAATTCAA GACCACTGTC CCGAATGTAG AATCAAAGTC AACTCAAGAA 
AAGGAAAGAG AGAAGGAGCC GTCCAGAGAA CGAGATATAG CAAAGGAAAT GAAATCAAAG 
GAAAATGTTA AAGGAGGAGA GAAAACACCA GTTTCTGGGT CCTTGAAATC ACCTGTTCCC 
CGATCAGATA TTACAGAGCC TGAAAGGGAA CAGAAACGTC GCAAAATTGA TACCCATCCT 
TCTCCATCAC ATTCCTCAAC AGTAAAGATG GTTTATTTCC AGGTTACAGC CATACTTCCC 
AAAGTTCCTC TGGGTTCTGA GAACTATGCC AGCTCACCTG TCATCTCCAT TCATTTTCTA 
CAGGACAGTC TCATCGAACT CAAGGAGTCT TCAGCAAAGC TCTACATTAA TCATACTCCT 
CCACCACTGT CCAAGAGTAA GGAGAGAGAA ATGGACAAGA AAGATTTGGA CAAGTCAAGG 
GAAAGATCCA GAGAAAGAGA AAAAAAAGAT GAAAAGGACA GGAAAGAGCG GAAAAGGGAT 
CACTCAAACA ATGACCGAGA AGTGCCACCG GACTTAACCA AGAGGCGGAA AGAGGAAAAT 
GGAACAATGG GGGTTTCAAA GCACAAAAGT GAAAGTCCAT GTGAGTCTCC TTATCCAAAT 
GAGAAAGACA AGGAAAAAAA TAAGTCAAAA TCTTCAGGCA AAGAAAAAGG CGGCGACTCA 
TTTAAATCTG AAAAGATGGA TAAAATCTCC TCTGGTGGCA AAAAGGAGTC CAGGCATGAT 
AAAGAAAAGA TAGAAAAGAA AGAGAAACGG GACAGTTCAG GAGGAAAGGA AGAGAAGAAA 
CAGTCCTCGG ACAAGCACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025132246.1
CDS	86..4969
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYVCFEILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKARETKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKMVYFQVTAILPKVPLGSENYASSPVISIHFLQDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKQSSDKHR

Target ORF information:

RefSeq Version	XM_025276461.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025276461.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATGTAT GTTTTGAGAT CTTGTCACAA ATACAGAAGT ATGATAACTT GATAACACCT 
GTAGTAGATT CATTGAAATA CCTCACTTCG TTGAATTATG ACGTCTTGGC CTATTGTATC 
ATTGAAGCTT TAGCTAATCC AGAAAAGGAG AAAATGAAAC ATGATGACAC AACCATCTCA 
AGTTGGCTTC AGAGTCTGGC TAGTTTCTGT GGTGCAGTTT TTCGTAAATA TCCAATTGAT 
CTTGCTGGTC TTCTTCAGTA TGTGGCTAAT CAGCTAAAGG CAGGCAAAAG TTTTGACCTG 
CTTATACTGA AAGAAGTGGT ACAAAAAATG GCAGGAATAG AAATTACAGA AGAAATGACA 
ATGGAGCAAC TAGAAGCCAT GACTGGTGGA GAGCAACTAA AAGCTGAGGG TGGTTATTTT 
GGCCAGATAA GAAACACGAA AAAATCCTCC CAGAGATTAA AGGATGCACT ATTAGACCAT 
GATCTTGCTC TTCCTCTCTG CTTGCTTATG GCTCAGCAGA GGAATGGGGT AATCTTTCAG 
GAAGGTGGAG AGAAACATTT GAAACTTGTG GGAAAACTCT ATGATCAGTG TCATGATACC 
CTGGTACAGT TTGGTGGGTT TTTAGCATCT AATCTAAGCA CAGAAGATTA TATAAAGCGA 
GTGCCTTCAA TTGATGTGCT CTGTAATGAA TTTCACACAC CTCATGATGC AGCATTTTTC 
CTGTCTAGGC CAATGTATGC ACACCATATT TCGTCAAAGT ATGACGAACT TAAAAAATCA 
GAGAAGGGAA GTAAACAGCA GCATAAAGTT CATAAGTACA TCACATCATG TGAAATGGTG 
ATGGCTCCTG TTCATGAAGC AGTGGTTTCC TTACACGTTT CCAAAGTCTG GGATGACATC 
AGTCCTCAAT TCTATGCCAC ATTCTGGTCA TTGACAATGT ATGACCTTGC AGTTCCACAT 
ACCAGCTATG AACGGGAAGT CAATAAACTT AAAATCCAGA TGAAAGCAAT TGATGACAAT 
CAGGAAATGC CTCCAAATAA AAAGAAAAAA GAGAAGGAGC GATGTACTGC CCTTCAGGAC 
AAGCTTCTCG AAGAAGAAAA GAAACAGATG GAACATGTAC AGCGAGTTCT GCAGAGACTG 
AAACTGGAAA AGGACAACTG GCTTTTAGCA AAATCTACCA AAAATGAGAC CATCACAAAA 
TTTCTACAGC TGTGTATATT TCCTCGATGT ATTTTTTCAG CAATTGATGC TGTTTACTGT 
GCTCGTTTTG TTGAATTGGT ACATCAACAG AAAACTCCAA ATTTTTCCAC ACTTCTTTGC 
TATGATCGAG TTTTCTCTGA TATAATTTAC ACAGTTGCAA GCTGTACTGA AAATGAAGCT 
AGTCGATATG GGAGATTCCT TTGCTGCATG TTAGAGACTG TGACCAGGTG GCACAGTGAT 
AGAGCCACAT ATGAAAAGGA ATGTGGAAAT TATCCAGGAT TCCTTACTAT ATTACGAGCA 
ACTGGATTTG ATGGTGGAAA TAAAGCTGAT CAATTAGACT ATGAAAATTT TCGACATGTT 
GTACACAAAT GGCATTACAA ACTAACCAAG GCCTCGGTAC ATTGCCTTGA AACAGGCGAA 
TATACTCACA TCAGGAATAT CTTGATTGTG CTAACAAAAA TACTTCCTTG GTACCCAAAA 
GTTTTGAATC TGGGTCAGGC ATTGGAAAGA AGAGTGCATA AAATCTGCCA AGAGGAAAAA 
GAGAAGAGGC CAGATCTATA TGCATTGGCT ATGGGCTACT CTGGGCAGTT GAAAAGTAGA 
AAGTCATACA TGATACCTGA AAATGAGTTT CATCACAAAG ACCCCCCTCC GAGGAATGCA 
GTTGCCAGTG TACAAAATGG GCCTGGTGGT GGGCCTTCTT CATCGTCGAT CGGAAGTGTA 
TCTAAGTCAG ACGAAAGCAG TACCGAGGAC ACTGATAAAT CAAGGGAGAG ATCTCAGTGT 
GGTGTGAAAG CTGTTAATAA AGCTTCTAGT GCCACACCAA AAGGGAATTC AAGTAATGGA 
AATAGTGGCT CTAACAGCAG CAAAACTGTT AAAGAAAATG ACAAAGAAAA AGGAAAAGAG 
AAAGAAAAAG AGAAAAAAGA AAAGACTCCA GCTACTACTC CAGAGGCCCG AGTACTTGGT 
AAAGATGGTA AAGAAAAACC AAAGGAAGAA CGGCCAAATA AAGATGAAAA AGCAAGAGAG 
ACAAAGGAAA GAACACCTAA ATCTGACAAA GAGAAAGAAA AATTCAAGAA GGAAGAAAAA 
GCTAAAGATG AGAAATTCAA GACCACTGTC CCGAATGTAG AATCAAAGTC AACTCAAGAA 
AAGGAAAGAG AGAAGGAGCC GTCCAGAGAA CGAGATATAG CAAAGGAAAT GAAATCAAAG 
GAAAATGTTA AAGGAGGAGA GAAAACACCA GTTTCTGGGT CCTTGAAATC ACCTGTTCCC 
CGATCAGATA TTACAGAGCC TGAAAGGGAA CAGAAACGTC GCAAAATTGA TACCCATCCT 
TCTCCATCAC ATTCCTCAAC AGTAAAGATG GTTTATTTCC AGGTTACAGC CATACTTCCC 
AAAGTTCCTC TGGGTTCTGA GAACTATGCC AGCTCACCTG TCATCTCCAT TCATTTTCTA 
CAGGACAGTC TCATCGAACT CAAGGAGTCT TCAGCAAAGC TCTACATTAA TCATACTCCT 
CCACCACTGT CCAAGAGTAA GGAGAGAGAA ATGGACAAGA AAGATTTGGA CAAGTCAAGG 
GAAAGATCCA GAGAAAGAGA AAAAAAAGAT GAAAAGGACA GGAAAGAGCG GAAAAGGGAT 
CACTCAAACA ATGACCGAGA AGTGCCACCG GACTTAACCA AGAGGCGGAA AGAGGAAAAT 
GGAACAATGG GGGTTTCAAA GCACAAAAGT GAAAGTCCAT GTGAGTCTCC TTATCCAAAT 
GAGAAAGACA AGGAAAAAAA TAAGTCAAAA TCTTCAGGCA AAGAAAAAGG CGGCGACTCA 
TTTAAATCTG AAAAGATGGA TAAAATCTCC TCTGGTGGCA AAAAGGAGTC CAGGCATGAT 
AAAGAAAAGA TAGAAAAGAA AGAGAAACGG GACAGTTCAG GAGGAAAGGA AGAGAAGAAA 
CAGTCCTCGG ACAAGCACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_025276460.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025132245.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu73755
Clone ID Related Accession (Same CDS sequence)	XM_025276461.1 , XM_025276460.1
Accession Version	XM_025276460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATGTAT GTTTTGAGAT CTTGTCACAA ATACAGAAGT ATGATAACTT GATAACACCT 
GTAGTAGATT CATTGAAATA CCTCACTTCG TTGAATTATG ACGTCTTGGC CTATTGTATC 
ATTGAAGCTT TAGCTAATCC AGAAAAGGAG AAAATGAAAC ATGATGACAC AACCATCTCA 
AGTTGGCTTC AGAGTCTGGC TAGTTTCTGT GGTGCAGTTT TTCGTAAATA TCCAATTGAT 
CTTGCTGGTC TTCTTCAGTA TGTGGCTAAT CAGCTAAAGG CAGGCAAAAG TTTTGACCTG 
CTTATACTGA AAGAAGTGGT ACAAAAAATG GCAGGAATAG AAATTACAGA AGAAATGACA 
ATGGAGCAAC TAGAAGCCAT GACTGGTGGA GAGCAACTAA AAGCTGAGGG TGGTTATTTT 
GGCCAGATAA GAAACACGAA AAAATCCTCC CAGAGATTAA AGGATGCACT ATTAGACCAT 
GATCTTGCTC TTCCTCTCTG CTTGCTTATG GCTCAGCAGA GGAATGGGGT AATCTTTCAG 
GAAGGTGGAG AGAAACATTT GAAACTTGTG GGAAAACTCT ATGATCAGTG TCATGATACC 
CTGGTACAGT TTGGTGGGTT TTTAGCATCT AATCTAAGCA CAGAAGATTA TATAAAGCGA 
GTGCCTTCAA TTGATGTGCT CTGTAATGAA TTTCACACAC CTCATGATGC AGCATTTTTC 
CTGTCTAGGC CAATGTATGC ACACCATATT TCGTCAAAGT ATGACGAACT TAAAAAATCA 
GAGAAGGGAA GTAAACAGCA GCATAAAGTT CATAAGTACA TCACATCATG TGAAATGGTG 
ATGGCTCCTG TTCATGAAGC AGTGGTTTCC TTACACGTTT CCAAAGTCTG GGATGACATC 
AGTCCTCAAT TCTATGCCAC ATTCTGGTCA TTGACAATGT ATGACCTTGC AGTTCCACAT 
ACCAGCTATG AACGGGAAGT CAATAAACTT AAAATCCAGA TGAAAGCAAT TGATGACAAT 
CAGGAAATGC CTCCAAATAA AAAGAAAAAA GAGAAGGAGC GATGTACTGC CCTTCAGGAC 
AAGCTTCTCG AAGAAGAAAA GAAACAGATG GAACATGTAC AGCGAGTTCT GCAGAGACTG 
AAACTGGAAA AGGACAACTG GCTTTTAGCA AAATCTACCA AAAATGAGAC CATCACAAAA 
TTTCTACAGC TGTGTATATT TCCTCGATGT ATTTTTTCAG CAATTGATGC TGTTTACTGT 
GCTCGTTTTG TTGAATTGGT ACATCAACAG AAAACTCCAA ATTTTTCCAC ACTTCTTTGC 
TATGATCGAG TTTTCTCTGA TATAATTTAC ACAGTTGCAA GCTGTACTGA AAATGAAGCT 
AGTCGATATG GGAGATTCCT TTGCTGCATG TTAGAGACTG TGACCAGGTG GCACAGTGAT 
AGAGCCACAT ATGAAAAGGA ATGTGGAAAT TATCCAGGAT TCCTTACTAT ATTACGAGCA 
ACTGGATTTG ATGGTGGAAA TAAAGCTGAT CAATTAGACT ATGAAAATTT TCGACATGTT 
GTACACAAAT GGCATTACAA ACTAACCAAG GCCTCGGTAC ATTGCCTTGA AACAGGCGAA 
TATACTCACA TCAGGAATAT CTTGATTGTG CTAACAAAAA TACTTCCTTG GTACCCAAAA 
GTTTTGAATC TGGGTCAGGC ATTGGAAAGA AGAGTGCATA AAATCTGCCA AGAGGAAAAA 
GAGAAGAGGC CAGATCTATA TGCATTGGCT ATGGGCTACT CTGGGCAGTT GAAAAGTAGA 
AAGTCATACA TGATACCTGA AAATGAGTTT CATCACAAAG ACCCCCCTCC GAGGAATGCA 
GTTGCCAGTG TACAAAATGG GCCTGGTGGT GGGCCTTCTT CATCGTCGAT CGGAAGTGTA 
TCTAAGTCAG ACGAAAGCAG TACCGAGGAC ACTGATAAAT CAAGGGAGAG ATCTCAGTGT 
GGTGTGAAAG CTGTTAATAA AGCTTCTAGT GCCACACCAA AAGGGAATTC AAGTAATGGA 
AATAGTGGCT CTAACAGCAG CAAAACTGTT AAAGAAAATG ACAAAGAAAA AGGAAAAGAG 
AAAGAAAAAG AGAAAAAAGA AAAGACTCCA GCTACTACTC CAGAGGCCCG AGTACTTGGT 
AAAGATGGTA AAGAAAAACC AAAGGAAGAA CGGCCAAATA AAGATGAAAA AGCAAGAGAG 
ACAAAGGAAA GAACACCTAA ATCTGACAAA GAGAAAGAAA AATTCAAGAA GGAAGAAAAA 
GCTAAAGATG AGAAATTCAA GACCACTGTC CCGAATGTAG AATCAAAGTC AACTCAAGAA 
AAGGAAAGAG AGAAGGAGCC GTCCAGAGAA CGAGATATAG CAAAGGAAAT GAAATCAAAG 
GAAAATGTTA AAGGAGGAGA GAAAACACCA GTTTCTGGGT CCTTGAAATC ACCTGTTCCC 
CGATCAGATA TTACAGAGCC TGAAAGGGAA CAGAAACGTC GCAAAATTGA TACCCATCCT 
TCTCCATCAC ATTCCTCAAC AGTAAAGATG GTTTATTTCC AGGTTACAGC CATACTTCCC 
AAAGTTCCTC TGGGTTCTGA GAACTATGCC AGCTCACCTG TCATCTCCAT TCATTTTCTA 
CAGGACAGTC TCATCGAACT CAAGGAGTCT TCAGCAAAGC TCTACATTAA TCATACTCCT 
CCACCACTGT CCAAGAGTAA GGAGAGAGAA ATGGACAAGA AAGATTTGGA CAAGTCAAGG 
GAAAGATCCA GAGAAAGAGA AAAAAAAGAT GAAAAGGACA GGAAAGAGCG GAAAAGGGAT 
CACTCAAACA ATGACCGAGA AGTGCCACCG GACTTAACCA AGAGGCGGAA AGAGGAAAAT 
GGAACAATGG GGGTTTCAAA GCACAAAAGT GAAAGTCCAT GTGAGTCTCC TTATCCAAAT 
GAGAAAGACA AGGAAAAAAA TAAGTCAAAA TCTTCAGGCA AAGAAAAAGG CGGCGACTCA 
TTTAAATCTG AAAAGATGGA TAAAATCTCC TCTGGTGGCA AAAAGGAGTC CAGGCATGAT 
AAAGAAAAGA TAGAAAAGAA AGAGAAACGG GACAGTTCAG GAGGAAAGGA AGAGAAGAAA 
CAGTCCTCGG ACAAGCACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025132245.1
CDS	86..4969
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYVCFEILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKARETKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKMVYFQVTAILPKVPLGSENYASSPVISIHFLQDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKQSSDKHR

Target ORF information:

RefSeq Version	XM_025276460.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025276460.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATGTAT GTTTTGAGAT CTTGTCACAA ATACAGAAGT ATGATAACTT GATAACACCT 
GTAGTAGATT CATTGAAATA CCTCACTTCG TTGAATTATG ACGTCTTGGC CTATTGTATC 
ATTGAAGCTT TAGCTAATCC AGAAAAGGAG AAAATGAAAC ATGATGACAC AACCATCTCA 
AGTTGGCTTC AGAGTCTGGC TAGTTTCTGT GGTGCAGTTT TTCGTAAATA TCCAATTGAT 
CTTGCTGGTC TTCTTCAGTA TGTGGCTAAT CAGCTAAAGG CAGGCAAAAG TTTTGACCTG 
CTTATACTGA AAGAAGTGGT ACAAAAAATG GCAGGAATAG AAATTACAGA AGAAATGACA 
ATGGAGCAAC TAGAAGCCAT GACTGGTGGA GAGCAACTAA AAGCTGAGGG TGGTTATTTT 
GGCCAGATAA GAAACACGAA AAAATCCTCC CAGAGATTAA AGGATGCACT ATTAGACCAT 
GATCTTGCTC TTCCTCTCTG CTTGCTTATG GCTCAGCAGA GGAATGGGGT AATCTTTCAG 
GAAGGTGGAG AGAAACATTT GAAACTTGTG GGAAAACTCT ATGATCAGTG TCATGATACC 
CTGGTACAGT TTGGTGGGTT TTTAGCATCT AATCTAAGCA CAGAAGATTA TATAAAGCGA 
GTGCCTTCAA TTGATGTGCT CTGTAATGAA TTTCACACAC CTCATGATGC AGCATTTTTC 
CTGTCTAGGC CAATGTATGC ACACCATATT TCGTCAAAGT ATGACGAACT TAAAAAATCA 
GAGAAGGGAA GTAAACAGCA GCATAAAGTT CATAAGTACA TCACATCATG TGAAATGGTG 
ATGGCTCCTG TTCATGAAGC AGTGGTTTCC TTACACGTTT CCAAAGTCTG GGATGACATC 
AGTCCTCAAT TCTATGCCAC ATTCTGGTCA TTGACAATGT ATGACCTTGC AGTTCCACAT 
ACCAGCTATG AACGGGAAGT CAATAAACTT AAAATCCAGA TGAAAGCAAT TGATGACAAT 
CAGGAAATGC CTCCAAATAA AAAGAAAAAA GAGAAGGAGC GATGTACTGC CCTTCAGGAC 
AAGCTTCTCG AAGAAGAAAA GAAACAGATG GAACATGTAC AGCGAGTTCT GCAGAGACTG 
AAACTGGAAA AGGACAACTG GCTTTTAGCA AAATCTACCA AAAATGAGAC CATCACAAAA 
TTTCTACAGC TGTGTATATT TCCTCGATGT ATTTTTTCAG CAATTGATGC TGTTTACTGT 
GCTCGTTTTG TTGAATTGGT ACATCAACAG AAAACTCCAA ATTTTTCCAC ACTTCTTTGC 
TATGATCGAG TTTTCTCTGA TATAATTTAC ACAGTTGCAA GCTGTACTGA AAATGAAGCT 
AGTCGATATG GGAGATTCCT TTGCTGCATG TTAGAGACTG TGACCAGGTG GCACAGTGAT 
AGAGCCACAT ATGAAAAGGA ATGTGGAAAT TATCCAGGAT TCCTTACTAT ATTACGAGCA 
ACTGGATTTG ATGGTGGAAA TAAAGCTGAT CAATTAGACT ATGAAAATTT TCGACATGTT 
GTACACAAAT GGCATTACAA ACTAACCAAG GCCTCGGTAC ATTGCCTTGA AACAGGCGAA 
TATACTCACA TCAGGAATAT CTTGATTGTG CTAACAAAAA TACTTCCTTG GTACCCAAAA 
GTTTTGAATC TGGGTCAGGC ATTGGAAAGA AGAGTGCATA AAATCTGCCA AGAGGAAAAA 
GAGAAGAGGC CAGATCTATA TGCATTGGCT ATGGGCTACT CTGGGCAGTT GAAAAGTAGA 
AAGTCATACA TGATACCTGA AAATGAGTTT CATCACAAAG ACCCCCCTCC GAGGAATGCA 
GTTGCCAGTG TACAAAATGG GCCTGGTGGT GGGCCTTCTT CATCGTCGAT CGGAAGTGTA 
TCTAAGTCAG ACGAAAGCAG TACCGAGGAC ACTGATAAAT CAAGGGAGAG ATCTCAGTGT 
GGTGTGAAAG CTGTTAATAA AGCTTCTAGT GCCACACCAA AAGGGAATTC AAGTAATGGA 
AATAGTGGCT CTAACAGCAG CAAAACTGTT AAAGAAAATG ACAAAGAAAA AGGAAAAGAG 
AAAGAAAAAG AGAAAAAAGA AAAGACTCCA GCTACTACTC CAGAGGCCCG AGTACTTGGT 
AAAGATGGTA AAGAAAAACC AAAGGAAGAA CGGCCAAATA AAGATGAAAA AGCAAGAGAG 
ACAAAGGAAA GAACACCTAA ATCTGACAAA GAGAAAGAAA AATTCAAGAA GGAAGAAAAA 
GCTAAAGATG AGAAATTCAA GACCACTGTC CCGAATGTAG AATCAAAGTC AACTCAAGAA 
AAGGAAAGAG AGAAGGAGCC GTCCAGAGAA CGAGATATAG CAAAGGAAAT GAAATCAAAG 
GAAAATGTTA AAGGAGGAGA GAAAACACCA GTTTCTGGGT CCTTGAAATC ACCTGTTCCC 
CGATCAGATA TTACAGAGCC TGAAAGGGAA CAGAAACGTC GCAAAATTGA TACCCATCCT 
TCTCCATCAC ATTCCTCAAC AGTAAAGATG GTTTATTTCC AGGTTACAGC CATACTTCCC 
AAAGTTCCTC TGGGTTCTGA GAACTATGCC AGCTCACCTG TCATCTCCAT TCATTTTCTA 
CAGGACAGTC TCATCGAACT CAAGGAGTCT TCAGCAAAGC TCTACATTAA TCATACTCCT 
CCACCACTGT CCAAGAGTAA GGAGAGAGAA ATGGACAAGA AAGATTTGGA CAAGTCAAGG 
GAAAGATCCA GAGAAAGAGA AAAAAAAGAT GAAAAGGACA GGAAAGAGCG GAAAAGGGAT 
CACTCAAACA ATGACCGAGA AGTGCCACCG GACTTAACCA AGAGGCGGAA AGAGGAAAAT 
GGAACAATGG GGGTTTCAAA GCACAAAAGT GAAAGTCCAT GTGAGTCTCC TTATCCAAAT 
GAGAAAGACA AGGAAAAAAA TAAGTCAAAA TCTTCAGGCA AAGAAAAAGG CGGCGACTCA 
TTTAAATCTG AAAAGATGGA TAAAATCTCC TCTGGTGGCA AAAAGGAGTC CAGGCATGAT 
AAAGAAAAGA TAGAAAAGAA AGAGAAACGG GACAGTTCAG GAGGAAAGGA AGAGAAGAAA 
CAGTCCTCGG ACAAGCACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_025276462.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025132247.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X6, mRNA.

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CloneID	OBu73756
Clone ID Related Accession (Same CDS sequence)	XM_025276462.1
Accession Version	XM_025276462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAC AAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGATGGT TTATTTCCAG GTTACAGCCA TACTTCCCAA AGTTCCTCTG 
GGTTCTGAGA ACTATGCCAG CTCACCTGTC ATCTCCATTC ATTTTCTACA GGACAGTCTC 
ATCGAACTCA AGGAGTCTTC AGCAAAGCTC TACATTAATC ATACTCCTCC ACCACTGTCC 
AAGAGTAAGG AGAGAGAAAT GGACAAGAAA GATTTGGACA AGTCAAGGGA AAGATCCAGA 
GAAAGAGAAA AAAAAGATGA AAAGGACAGG AAAGAGCGGA AAAGGGATCA CTCAAACAAT 
GACCGAGAAG TGCCACCGGA CTTAACCAAG AGGCGGAAAG AGGAAAATGG AACAATGGGG 
GTTTCAAAGC ACAAAAGTGA AAGTCCATGT GAGTCTCCTT ATCCAAATGA GAAAGACAAG 
GAAAAAAATA AGTCAAAATC TTCAGGCAAA GAAAAAGGCG GCGACTCATT TAAATCTGAA 
AAGATGGATA AAATCTCCTC TGGTGGCAAA AAGGAGTCCA GGCATGATAA AGAAAAGATA 
GAAAAGAAAG AGAAACGGGA CAGTTCAGGA GGAAAGGAAG AGAAGAAACA TCATAAGTCC 
TCGGACAAGC ACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025132247.1
CDS	86..4963
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKARETKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKMVYFQVTAILPKVPLGSENYASSPVISIHFLQDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKHHKSSDKHR

Target ORF information:

RefSeq Version	XM_025276462.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025276462.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAC AAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGATGGT TTATTTCCAG GTTACAGCCA TACTTCCCAA AGTTCCTCTG 
GGTTCTGAGA ACTATGCCAG CTCACCTGTC ATCTCCATTC ATTTTCTACA GGACAGTCTC 
ATCGAACTCA AGGAGTCTTC AGCAAAGCTC TACATTAATC ATACTCCTCC ACCACTGTCC 
AAGAGTAAGG AGAGAGAAAT GGACAAGAAA GATTTGGACA AGTCAAGGGA AAGATCCAGA 
GAAAGAGAAA AAAAAGATGA AAAGGACAGG AAAGAGCGGA AAAGGGATCA CTCAAACAAT 
GACCGAGAAG TGCCACCGGA CTTAACCAAG AGGCGGAAAG AGGAAAATGG AACAATGGGG 
GTTTCAAAGC ACAAAAGTGA AAGTCCATGT GAGTCTCCTT ATCCAAATGA GAAAGACAAG 
GAAAAAAATA AGTCAAAATC TTCAGGCAAA GAAAAAGGCG GCGACTCATT TAAATCTGAA 
AAGATGGATA AAATCTCCTC TGGTGGCAAA AAGGAGTCCA GGCATGATAA AGAAAAGATA 
GAAAAGAAAG AGAAACGGGA CAGTTCAGGA GGAAAGGAAG AGAAGAAACA TCATAAGTCC 
TCGGACAAGC ACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_025276463.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025132248.1 Bubalus bubalis THO complex 2 (THOC2), transcript variant X7, mRNA.

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CloneID	OBu73757
Clone ID Related Accession (Same CDS sequence)	XM_025276463.1
Accession Version	XM_025276463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATGTAT GTTTTGAGAT CTTGTCACAA ATACAGAAGT ATGATAACTT GATAACACCT 
GTAGTAGATT CATTGAAATA CCTCACTTCG TTGAATTATG ACGTCTTGGC CTATTGTATC 
ATTGAAGCTT TAGCTAATCC AGAAAAGGAG AAAATGAAAC ATGATGACAC AACCATCTCA 
AGTTGGCTTC AGAGTCTGGC TAGTTTCTGT GGTGCAGTTT TTCGTAAATA TCCAATTGAT 
CTTGCTGGTC TTCTTCAGTA TGTGGCTAAT CAGCTAAAGG CAGGCAAAAG TTTTGACCTG 
CTTATACTGA AAGAAGTGGT ACAAAAAATG GCAGGAATAG AAATTACAGA AGAAATGACA 
ATGGAGCAAC TAGAAGCCAT GACTGGTGGA GAGCAACTAA AAGCTGAGGG TGGTTATTTT 
GGCCAGATAA GAAACACGAA AAAATCCTCC CAGAGATTAA AGGATGCACT ATTAGACCAT 
GATCTTGCTC TTCCTCTCTG CTTGCTTATG GCTCAGCAGA GGAATGGGGT AATCTTTCAG 
GAAGGTGGAG AGAAACATTT GAAACTTGTG GGAAAACTCT ATGATCAGTG TCATGATACC 
CTGGTACAGT TTGGTGGGTT TTTAGCATCT AATCTAAGCA CAGAAGATTA TATAAAGCGA 
GTGCCTTCAA TTGATGTGCT CTGTAATGAA TTTCACACAC CTCATGATGC AGCATTTTTC 
CTGTCTAGGC CAATGTATGC ACACCATATT TCGTCAAAGT ATGACGAACT TAAAAAATCA 
GAGAAGGGAA GTAAACAGCA GCATAAAGTT CATAAGTACA TCACATCATG TGAAATGGTG 
ATGGCTCCTG TTCATGAAGC AGTGGTTTCC TTACACGTTT CCAAAGTCTG GGATGACATC 
AGTCCTCAAT TCTATGCCAC ATTCTGGTCA TTGACAATGT ATGACCTTGC AGTTCCACAT 
ACCAGCTATG AACGGGAAGT CAATAAACTT AAAATCCAGA TGAAAGCAAT TGATGACAAT 
CAGGAAATGC CTCCAAATAA AAAGAAAAAA GAGAAGGAGC GATGTACTGC CCTTCAGGAC 
AAGCTTCTCG AAGAAGAAAA GAAACAGATG GAACATGTAC AGCGAGTTCT GCAGAGACTG 
AAACTGGAAA AGGACAACTG GCTTTTAGCA AAATCTACCA AAAATGAGAC CATCACAAAA 
TTTCTACAGC TGTGTATATT TCCTCGATGT ATTTTTTCAG CAATTGATGC TGTTTACTGT 
GCTCGTTTTG TTGAATTGGT ACATCAACAG AAAACTCCAA ATTTTTCCAC ACTTCTTTGC 
TATGATCGAG TTTTCTCTGA TATAATTTAC ACAGTTGCAA GCTGTACTGA AAATGAAGCT 
AGTCGATATG GGAGATTCCT TTGCTGCATG TTAGAGACTG TGACCAGGTG GCACAGTGAT 
AGAGCCACAT ATGAAAAGGA ATGTGGAAAT TATCCAGGAT TCCTTACTAT ATTACGAGCA 
ACTGGATTTG ATGGTGGAAA TAAAGCTGAT CAATTAGACT ATGAAAATTT TCGACATGTT 
GTACACAAAT GGCATTACAA ACTAACCAAG GCCTCGGTAC ATTGCCTTGA AACAGGCGAA 
TATACTCACA TCAGGAATAT CTTGATTGTG CTAACAAAAA TACTTCCTTG GTACCCAAAA 
GTTTTGAATC TGGGTCAGGC ATTGGAAAGA AGAGTGCATA AAATCTGCCA AGAGGAAAAA 
GAGAAGAGGC CAGATCTATA TGCATTGGCT ATGGGCTACT CTGGGCAGTT GAAAAGTAGA 
AAGTCATACA TGATACCTGA AAATGAGTTT CATCACAAAG ACCCCCCTCC GAGGAATGCA 
GTTGCCAGTG TACAAAATGG GCCTGGTGGT GGGCCTTCTT CATCGTCGAT CGGAAGTGTA 
TCTAAGTCAG ACGAAAGCAG TACCGAGGAC ACTGATAAAT CAAGGGAGAG ATCTCAGTGT 
GGTGTGAAAG CTGTTAATAA AGCTTCTAGT GCCACACCAA AAGGGAATTC AAGTAATGGA 
AATAGTGGCT CTAACAGCAG CAAAACTGTT AAAGAAAATG ACAAAGAAAA AGGAAAAGAG 
AAAGAAAAAG AGAAAAAAGA AAAGACTCCA GCTACTACTC CAGAGGCCCG AGTACTTGGT 
AAAGATGGTA AAGAAAAACC AAAGGAAGAA CGGCCAAATA AAGATGAAAA AGCAAGAGAG 
ACAAAGGAAA GAACACCTAA ATCTGACAAA GAGAAAGAAA AATTCAAGAA GGAAGAAAAA 
GCTAAAGATG AGAAATTCAA GACCACTGTC CCGAATGTAG AATCAAAGTC AACTCAAGAA 
AAGGAAAGAG AGAAGGAGCC GTCCAGAGAA CGAGATATAG CAAAGGAAAT GAAATCAAAG 
GAAAATGTTA AAGGAGGAGA GAAAACACCA GTTTCTGGGT CCTTGAAATC ACCTGTTCCC 
CGATCAGATA TTACAGAGCC TGAAAGGGAA CAGAAACGTC GCAAAATTGA TACCCATCCT 
TCTCCATCAC ATTCCTCAAC AGTAAAGGTT ACAGCCATAC TTCCCAAAGT TCCTCTGGGT 
TCTGAGAACT ATGCCAGCTC ACCTGTCATC TCCATTCATT TTCTACAGGA CAGTCTCATC 
GAACTCAAGG AGTCTTCAGC AAAGCTCTAC ATTAATCATA CTCCTCCACC ACTGTCCAAG 
AGTAAGGAGA GAGAAATGGA CAAGAAAGAT TTGGACAAGT CAAGGGAAAG ATCCAGAGAA 
AGAGAAAAAA AAGATGAAAA GGACAGGAAA GAGCGGAAAA GGGATCACTC AAACAATGAC 
CGAGAAGTGC CACCGGACTT AACCAAGAGG CGGAAAGAGG AAAATGGAAC AATGGGGGTT 
TCAAAGCACA AAAGTGAAAG TCCATGTGAG TCTCCTTATC CAAATGAGAA AGACAAGGAA 
AAAAATAAGT CAAAATCTTC AGGCAAAGAA AAAGGCGGCG ACTCATTTAA ATCTGAAAAG 
ATGGATAAAA TCTCCTCTGG TGGCAAAAAG GAGTCCAGGC ATGATAAAGA AAAGATAGAA 
AAGAAAGAGA AACGGGACAG TTCAGGAGGA AAGGAAGAGA AGAAACATCA TAAGTCCTCG 
GACAAGCACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025132248.1
CDS	86..4960
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYVCFEILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKARETKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKVTAILPKVPLGSENYASSPVISIHFLQDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKHHKSSDKHR

Target ORF information:

RefSeq Version	XM_025276463.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025276463.1

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATGTAT GTTTTGAGAT CTTGTCACAA ATACAGAAGT ATGATAACTT GATAACACCT 
GTAGTAGATT CATTGAAATA CCTCACTTCG TTGAATTATG ACGTCTTGGC CTATTGTATC 
ATTGAAGCTT TAGCTAATCC AGAAAAGGAG AAAATGAAAC ATGATGACAC AACCATCTCA 
AGTTGGCTTC AGAGTCTGGC TAGTTTCTGT GGTGCAGTTT TTCGTAAATA TCCAATTGAT 
CTTGCTGGTC TTCTTCAGTA TGTGGCTAAT CAGCTAAAGG CAGGCAAAAG TTTTGACCTG 
CTTATACTGA AAGAAGTGGT ACAAAAAATG GCAGGAATAG AAATTACAGA AGAAATGACA 
ATGGAGCAAC TAGAAGCCAT GACTGGTGGA GAGCAACTAA AAGCTGAGGG TGGTTATTTT 
GGCCAGATAA GAAACACGAA AAAATCCTCC CAGAGATTAA AGGATGCACT ATTAGACCAT 
GATCTTGCTC TTCCTCTCTG CTTGCTTATG GCTCAGCAGA GGAATGGGGT AATCTTTCAG 
GAAGGTGGAG AGAAACATTT GAAACTTGTG GGAAAACTCT ATGATCAGTG TCATGATACC 
CTGGTACAGT TTGGTGGGTT TTTAGCATCT AATCTAAGCA CAGAAGATTA TATAAAGCGA 
GTGCCTTCAA TTGATGTGCT CTGTAATGAA TTTCACACAC CTCATGATGC AGCATTTTTC 
CTGTCTAGGC CAATGTATGC ACACCATATT TCGTCAAAGT ATGACGAACT TAAAAAATCA 
GAGAAGGGAA GTAAACAGCA GCATAAAGTT CATAAGTACA TCACATCATG TGAAATGGTG 
ATGGCTCCTG TTCATGAAGC AGTGGTTTCC TTACACGTTT CCAAAGTCTG GGATGACATC 
AGTCCTCAAT TCTATGCCAC ATTCTGGTCA TTGACAATGT ATGACCTTGC AGTTCCACAT 
ACCAGCTATG AACGGGAAGT CAATAAACTT AAAATCCAGA TGAAAGCAAT TGATGACAAT 
CAGGAAATGC CTCCAAATAA AAAGAAAAAA GAGAAGGAGC GATGTACTGC CCTTCAGGAC 
AAGCTTCTCG AAGAAGAAAA GAAACAGATG GAACATGTAC AGCGAGTTCT GCAGAGACTG 
AAACTGGAAA AGGACAACTG GCTTTTAGCA AAATCTACCA AAAATGAGAC CATCACAAAA 
TTTCTACAGC TGTGTATATT TCCTCGATGT ATTTTTTCAG CAATTGATGC TGTTTACTGT 
GCTCGTTTTG TTGAATTGGT ACATCAACAG AAAACTCCAA ATTTTTCCAC ACTTCTTTGC 
TATGATCGAG TTTTCTCTGA TATAATTTAC ACAGTTGCAA GCTGTACTGA AAATGAAGCT 
AGTCGATATG GGAGATTCCT TTGCTGCATG TTAGAGACTG TGACCAGGTG GCACAGTGAT 
AGAGCCACAT ATGAAAAGGA ATGTGGAAAT TATCCAGGAT TCCTTACTAT ATTACGAGCA 
ACTGGATTTG ATGGTGGAAA TAAAGCTGAT CAATTAGACT ATGAAAATTT TCGACATGTT 
GTACACAAAT GGCATTACAA ACTAACCAAG GCCTCGGTAC ATTGCCTTGA AACAGGCGAA 
TATACTCACA TCAGGAATAT CTTGATTGTG CTAACAAAAA TACTTCCTTG GTACCCAAAA 
GTTTTGAATC TGGGTCAGGC ATTGGAAAGA AGAGTGCATA AAATCTGCCA AGAGGAAAAA 
GAGAAGAGGC CAGATCTATA TGCATTGGCT ATGGGCTACT CTGGGCAGTT GAAAAGTAGA 
AAGTCATACA TGATACCTGA AAATGAGTTT CATCACAAAG ACCCCCCTCC GAGGAATGCA 
GTTGCCAGTG TACAAAATGG GCCTGGTGGT GGGCCTTCTT CATCGTCGAT CGGAAGTGTA 
TCTAAGTCAG ACGAAAGCAG TACCGAGGAC ACTGATAAAT CAAGGGAGAG ATCTCAGTGT 
GGTGTGAAAG CTGTTAATAA AGCTTCTAGT GCCACACCAA AAGGGAATTC AAGTAATGGA 
AATAGTGGCT CTAACAGCAG CAAAACTGTT AAAGAAAATG ACAAAGAAAA AGGAAAAGAG 
AAAGAAAAAG AGAAAAAAGA AAAGACTCCA GCTACTACTC CAGAGGCCCG AGTACTTGGT 
AAAGATGGTA AAGAAAAACC AAAGGAAGAA CGGCCAAATA AAGATGAAAA AGCAAGAGAG 
ACAAAGGAAA GAACACCTAA ATCTGACAAA GAGAAAGAAA AATTCAAGAA GGAAGAAAAA 
GCTAAAGATG AGAAATTCAA GACCACTGTC CCGAATGTAG AATCAAAGTC AACTCAAGAA 
AAGGAAAGAG AGAAGGAGCC GTCCAGAGAA CGAGATATAG CAAAGGAAAT GAAATCAAAG 
GAAAATGTTA AAGGAGGAGA GAAAACACCA GTTTCTGGGT CCTTGAAATC ACCTGTTCCC 
CGATCAGATA TTACAGAGCC TGAAAGGGAA CAGAAACGTC GCAAAATTGA TACCCATCCT 
TCTCCATCAC ATTCCTCAAC AGTAAAGGTT ACAGCCATAC TTCCCAAAGT TCCTCTGGGT 
TCTGAGAACT ATGCCAGCTC ACCTGTCATC TCCATTCATT TTCTACAGGA CAGTCTCATC 
GAACTCAAGG AGTCTTCAGC AAAGCTCTAC ATTAATCATA CTCCTCCACC ACTGTCCAAG 
AGTAAGGAGA GAGAAATGGA CAAGAAAGAT TTGGACAAGT CAAGGGAAAG ATCCAGAGAA 
AGAGAAAAAA AAGATGAAAA GGACAGGAAA GAGCGGAAAA GGGATCACTC AAACAATGAC 
CGAGAAGTGC CACCGGACTT AACCAAGAGG CGGAAAGAGG AAAATGGAAC AATGGGGGTT 
TCAAAGCACA AAAGTGAAAG TCCATGTGAG TCTCCTTATC CAAATGAGAA AGACAAGGAA 
AAAAATAAGT CAAAATCTTC AGGCAAAGAA AAAGGCGGCG ACTCATTTAA ATCTGAAAAG 
ATGGATAAAA TCTCCTCTGG TGGCAAAAAG GAGTCCAGGC ATGATAAAGA AAAGATAGAA 
AAGAAAGAGA AACGGGACAG TTCAGGAGGA AAGGAAGAGA AGAAACATCA TAAGTCCTCG 
GACAAGCACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_006064994.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065056.2 Bubalus bubalis THO complex 2 (THOC2), transcript variant X8, mRNA.

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CloneID	OBu73758
Clone ID Related Accession (Same CDS sequence)	XM_006064994.2
Accession Version	XM_006064994.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006064994.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAC AAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGACAG TCTCATCGAA CTCAAGGAGT CTTCAGCAAA GCTCTACATT 
AATCATACTC CTCCACCACT GTCCAAGAGT AAGGAGAGAG AAATGGACAA GAAAGATTTG 
GACAAGTCAA GGGAAAGATC CAGAGAAAGA GAAAAAAAAG ATGAAAAGGA CAGGAAAGAG 
CGGAAAAGGG ATCACTCAAA CAATGACCGA GAAGTGCCAC CGGACTTAAC CAAGAGGCGG 
AAAGAGGAAA ATGGAACAAT GGGGGTTTCA AAGCACAAAA GTGAAAGTCC ATGTGAGTCT 
CCTTATCCAA ATGAGAAAGA CAAGGAAAAA AATAAGTCAA AATCTTCAGG CAAAGAAAAA 
GGCGGCGACT CATTTAAATC TGAAAAGATG GATAAAATCT CCTCTGGTGG CAAAAAGGAG 
TCCAGGCATG ATAAAGAAAA GATAGAAAAG AAAGAGAAAC GGGACAGTTC AGGAGGAAAG 
GAAGAGAAGA AACAATATCC TTTTCATCTT ATAGATCTTT TTAATCAGTA TAATGGAAAA 
CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065056.2
CDS	86..4891
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKARETKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKQYPFHLIDLFNQYNGKL

Target ORF information:

RefSeq Version	XM_006064994.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064994.2

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAC AAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGACAG TCTCATCGAA CTCAAGGAGT CTTCAGCAAA GCTCTACATT 
AATCATACTC CTCCACCACT GTCCAAGAGT AAGGAGAGAG AAATGGACAA GAAAGATTTG 
GACAAGTCAA GGGAAAGATC CAGAGAAAGA GAAAAAAAAG ATGAAAAGGA CAGGAAAGAG 
CGGAAAAGGG ATCACTCAAA CAATGACCGA GAAGTGCCAC CGGACTTAAC CAAGAGGCGG 
AAAGAGGAAA ATGGAACAAT GGGGGTTTCA AAGCACAAAA GTGAAAGTCC ATGTGAGTCT 
CCTTATCCAA ATGAGAAAGA CAAGGAAAAA AATAAGTCAA AATCTTCAGG CAAAGAAAAA 
GGCGGCGACT CATTTAAATC TGAAAAGATG GATAAAATCT CCTCTGGTGG CAAAAAGGAG 
TCCAGGCATG ATAAAGAAAA GATAGAAAAG AAAGAGAAAC GGGACAGTTC AGGAGGAAAG 
GAAGAGAAGA AACAATATCC TTTTCATCTT ATAGATCTTT TTAATCAGTA TAATGGAAAA 
CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_006064996.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065058.2 Bubalus bubalis THO complex 2 (THOC2), transcript variant X9, mRNA.

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CloneID	OBu73759
Clone ID Related Accession (Same CDS sequence)	XM_006064996.2
Accession Version	XM_006064996.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006064996.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAC AAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGACAG TCTCATCGAA CTCAAGGAGT CTTCAGCAAA GCTCTACATT 
AATCATACTC CTCCACCACT GTCCAAGAGT AAGGAGAGAG AAATGGACAA GAAAGATTTG 
GACAAGTCAA GGGAAAGATC CAGAGAAAGA GAAAAAAAAG ATGAAAAGGA CAGGAAAGAG 
CGGAAAAGGG ATCACTCAAA CAATGACCGA GAAGTGCCAC CGGACTTAAC CAAGAGGCGG 
AAAGAGGAAA ATGGAACAAT GGGGGTTTCA AAGCACAAAA GTGAAAGTCC ATGTGAGTCT 
CCTTATCCAA ATGAGAAAGA CAAGGAAAAA AATAAGTCAA AATCTTCAGG CAAAGAAAAA 
GGCGGCGACT CATTTAAATC TGAAAAGATG GATAAAATCT CCTCTGGTGG CAAAAAGGAG 
TCCAGGCATG ATAAAGAAAA GATAGAAAAG AAAGAGAAAC GGGACAGTTC AGGAGGAAAG 
GAAGAGAAGA AACATCATAA GTCCTCGGAC AAGCACAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065058.2
CDS	86..4867
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKARETKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKHHKSSDKHR

Target ORF information:

RefSeq Version	XM_006064996.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064996.2

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAGCA AAACTGTTAA AGAAAATGAC AAAGAAAAAG GAAAAGAGAA AGAAAAAGAG 
AAAAAAGAAA AGACTCCAGC TACTACTCCA GAGGCCCGAG TACTTGGTAA AGATGGTAAA 
GAAAAACCAA AGGAAGAACG GCCAAATAAA GATGAAAAAG CAAGAGAGAC AAAGGAAAGA 
ACACCTAAAT CTGACAAAGA GAAAGAAAAA TTCAAGAAGG AAGAAAAAGC TAAAGATGAG 
AAATTCAAGA CCACTGTCCC GAATGTAGAA TCAAAGTCAA CTCAAGAAAA GGAAAGAGAG 
AAGGAGCCGT CCAGAGAACG AGATATAGCA AAGGAAATGA AATCAAAGGA AAATGTTAAA 
GGAGGAGAGA AAACACCAGT TTCTGGGTCC TTGAAATCAC CTGTTCCCCG ATCAGATATT 
ACAGAGCCTG AAAGGGAACA GAAACGTCGC AAAATTGATA CCCATCCTTC TCCATCACAT 
TCCTCAACAG TAAAGGACAG TCTCATCGAA CTCAAGGAGT CTTCAGCAAA GCTCTACATT 
AATCATACTC CTCCACCACT GTCCAAGAGT AAGGAGAGAG AAATGGACAA GAAAGATTTG 
GACAAGTCAA GGGAAAGATC CAGAGAAAGA GAAAAAAAAG ATGAAAAGGA CAGGAAAGAG 
CGGAAAAGGG ATCACTCAAA CAATGACCGA GAAGTGCCAC CGGACTTAAC CAAGAGGCGG 
AAAGAGGAAA ATGGAACAAT GGGGGTTTCA AAGCACAAAA GTGAAAGTCC ATGTGAGTCT 
CCTTATCCAA ATGAGAAAGA CAAGGAAAAA AATAAGTCAA AATCTTCAGG CAAAGAAAAA 
GGCGGCGACT CATTTAAATC TGAAAAGATG GATAAAATCT CCTCTGGTGG CAAAAAGGAG 
TCCAGGCATG ATAAAGAAAA GATAGAAAAG AAAGAGAAAC GGGACAGTTC AGGAGGAAAG 
GAAGAGAAGA AACATCATAA GTCCTCGGAC AAGCACAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THOC2 ( XM_006064995.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065057.2 Bubalus bubalis THO complex 2 (THOC2), transcript variant X10, mRNA.

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CloneID	OBu73760
Clone ID Related Accession (Same CDS sequence)	XM_006064995.2
Accession Version	XM_006064995.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	THO complex subunit 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006064995.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAAAA CTGTTAAAGA AAATGACAAA GAAAAAGGAA AAGAGAAAGA AAAAGAGAAA 
AAAGAAAAGA CTCCAGCTAC TACTCCAGAG GCCCGAGTAC TTGGTAAAGA TGGTAAAGAA 
AAACCAAAGG AAGAACGGCC AAATAAAGAT GAAAAAGCAA GAGAGACAAA GGAAAGAACA 
CCTAAATCTG ACAAAGAGAA AGAAAAATTC AAGAAGGAAG AAAAAGCTAA AGATGAGAAA 
TTCAAGACCA CTGTCCCGAA TGTAGAATCA AAGTCAACTC AAGAAAAGGA AAGAGAGAAG 
GAGCCGTCCA GAGAACGAGA TATAGCAAAG GAAATGAAAT CAAAGGAAAA TGTTAAAGGA 
GGAGAGAAAA CACCAGTTTC TGGGTCCTTG AAATCACCTG TTCCCCGATC AGATATTACA 
GAGCCTGAAA GGGAACAGAA ACGTCGCAAA ATTGATACCC ATCCTTCTCC ATCACATTCC 
TCAACAGTAA AGGACAGTCT CATCGAACTC AAGGAGTCTT CAGCAAAGCT CTACATTAAT 
CATACTCCTC CACCACTGTC CAAGAGTAAG GAGAGAGAAA TGGACAAGAA AGATTTGGAC 
AAGTCAAGGG AAAGATCCAG AGAAAGAGAA AAAAAAGATG AAAAGGACAG GAAAGAGCGG 
AAAAGGGATC ACTCAAACAA TGACCGAGAA GTGCCACCGG ACTTAACCAA GAGGCGGAAA 
GAGGAAAATG GAACAATGGG GGTTTCAAAG CACAAAAGTG AAAGTCCATG TGAGTCTCCT 
TATCCAAATG AGAAAGACAA GGAAAAAAAT AAGTCAAAAT CTTCAGGCAA AGAAAAAGGC 
GGCGACTCAT TTAAATCTGA AAAGATGGAT AAAATCTCCT CTGGTGGCAA AAAGGAGTCC 
AGGCATGATA AAGAAAAGAT AGAAAAGAAA GAGAAACGGG ACAGTTCAGG AGGAAAGGAA 
GAGAAGAAAC ATCATAAGTC CTCGGACAAG CACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065057.2
CDS	86..4864
Translation	MAAATVVVPVEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHKREIVEAKQIVRKLTMVVLSSDKIDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQHAQSIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVSALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKEKMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKIQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASVQNGPGGGPSSSSIGSVSKSDESSTEDTDKSRERSQCGVKAVNKASSATPKGNSSNGNSGSNSKTVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKARETKERTPKSDKEKEKFKKEEKAKDEKFKTTVPNVESKSTQEKEREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDITEPEREQKRRKIDTHPSPSHSSTVKDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGGDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKHHKSSDKHR

Target ORF information:

RefSeq Version	XM_006064995.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis THO complex 2 (THOC2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064995.2

ORF Insert Sequence:

ATGGCGGCCG CAACTGTGGT GGTTCCTGTG GAGTGGATAA AGAACTGGGA GAAATCAGGG 
AGAGGCGAAT TTTTACATTT ATGCCGGATC CTCAGTGAAA ATAAAAGCCA CGATAGTTCA 
ACTTACAGAG ATTTCCAGCA AGCTCTTTAT GAATTATCAT ACCATGTCAT TAAAGGAAAT 
CTAAAGCATG AACAGGCATC TAATGTTCTT AGTGACATCA GTGAATTTCG TGAGGATATG 
CCCTCCATTC TTGCTGATGT ATTCTGCATA TTAGATATTG AAACAAATTG TTTAGAAGAA 
AAAAGCAAGA GAGATTATTT TACACAATTG GTATTAGCAT GTTTGTATTT AGTTTCAGAC 
ACAGTTCTAA AGGAACGCTT GGATCCAGAA ACATTGGAAT CATTAGGGCT TATCAAACAA 
TCACAGCAAT TCAATCAAAA GTCAGTTAAA ATCAAGACAA AACTCTTTTA TAAGCAGCAA 
AAATTTAATT TGTTAAGAGA AGAAAATGAA GGTTATGCCA AGCTGATTGC TGAATTGGGG 
CAAGATTTAT CTGGAAATAT TACTAGTGAT TTAATCTTAG AAAATATCAA ATCTTTAATA 
GGATGCTTTA ATCTGGATCC CAATAGAGTT CTGGATGTCA TTTTAGAAGT GTTTGAATGC 
AGGCCAGAAC ACGATGACTT CTTTATATCT TTATTAGAAT CTTACATGAG TATGTGTGAA 
CCGCAAACAC TGTGTCATAT TCTTGGGTTC AAATTCAAGT TTTATCAGGA ACCAAATGGA 
GAGACTCCTT CATCTTTATA CAGAGTTGCA GCCGTACTTC TACAATTTAA CCTTATTGAT 
TTAGATGATC TTTATGTACA TCTTCTTCCA GCTGATAATT GCATTATGGA TGAACACAAA 
CGAGAAATTG TAGAAGCTAA GCAGATCGTT AGAAAACTTA CAATGGTTGT ATTGTCTTCT 
GATAAAATTG ACGAGCGAGA GAAAGAAAAG GAAAAAGAAG AGGAGAAAGT GGAGAAGCCA 
CCTGACAACC AAAAACTTGG TTTGTTGGAA GCTTTGTTAA AGATTGGTGA TTGGCAGCAT 
GCGCAGAGCA TTATGGATCA GATGCCTCCA TACTATGCAG CTTCACATAA ACTAATAGCC 
CTTGCTATTT GCAAGCTGAT TCATATAACT ATTGAGCCTC TCTATCGAAG AGTTGGCGTT 
CCTAAAGGTG CTAAAGGCTC ACCTGTTAGT GCTTTGCAAA ATAAGAGAGC ACCAAAACAA 
GCAGAGAGCT TTGAAGATTT GAGGAGAGAC GTCTTCAATA TGTTCTGTTA CCTTGGTCCC 
CACCTTTCTC ATGATCCCAT TTTATTCGCA AAAGTGGTGC GGATAGGCAA GTCATTTATG 
AAGGAGTTTC AGTCTGATGG AAGCAAACAA GAAGATAAAG AAAAAACGGA AGTTATCCTT 
AGCTGTTTGC TTAGCATTAC TGACCAGGTA TTACTTCCAT CTCTTTCTTT GATGGACTGC 
AATGCTTGTA TGTCTGAGGA ACTATGGGGA ATGTTTAAAA CATTTCCATA TCAGCATAGA 
TATCGTCTGT ATGGCCAGTG GAAGAATGAG ACTTACAACA GTCATCCACT TTTAGTAAAA 
GTTAAAGCTC AAACAATAGA CAGAGCCAAA TATATCATGA AGCGTCTAAC CAAGGAAAAT 
GTGAAGCCTT CTGGAAGACA AATTGGGAAG TTGAGCCACA GCAATCCAAC CATTTTGTTT 
GATTATATCT TGTCACAAAT ACAGAAGTAT GATAACTTGA TAACACCTGT AGTAGATTCA 
TTGAAATACC TCACTTCGTT GAATTATGAC GTCTTGGCCT ATTGTATCAT TGAAGCTTTA 
GCTAATCCAG AAAAGGAGAA AATGAAACAT GATGACACAA CCATCTCAAG TTGGCTTCAG 
AGTCTGGCTA GTTTCTGTGG TGCAGTTTTT CGTAAATATC CAATTGATCT TGCTGGTCTT 
CTTCAGTATG TGGCTAATCA GCTAAAGGCA GGCAAAAGTT TTGACCTGCT TATACTGAAA 
GAAGTGGTAC AAAAAATGGC AGGAATAGAA ATTACAGAAG AAATGACAAT GGAGCAACTA 
GAAGCCATGA CTGGTGGAGA GCAACTAAAA GCTGAGGGTG GTTATTTTGG CCAGATAAGA 
AACACGAAAA AATCCTCCCA GAGATTAAAG GATGCACTAT TAGACCATGA TCTTGCTCTT 
CCTCTCTGCT TGCTTATGGC TCAGCAGAGG AATGGGGTAA TCTTTCAGGA AGGTGGAGAG 
AAACATTTGA AACTTGTGGG AAAACTCTAT GATCAGTGTC ATGATACCCT GGTACAGTTT 
GGTGGGTTTT TAGCATCTAA TCTAAGCACA GAAGATTATA TAAAGCGAGT GCCTTCAATT 
GATGTGCTCT GTAATGAATT TCACACACCT CATGATGCAG CATTTTTCCT GTCTAGGCCA 
ATGTATGCAC ACCATATTTC GTCAAAGTAT GACGAACTTA AAAAATCAGA GAAGGGAAGT 
AAACAGCAGC ATAAAGTTCA TAAGTACATC ACATCATGTG AAATGGTGAT GGCTCCTGTT 
CATGAAGCAG TGGTTTCCTT ACACGTTTCC AAAGTCTGGG ATGACATCAG TCCTCAATTC 
TATGCCACAT TCTGGTCATT GACAATGTAT GACCTTGCAG TTCCACATAC CAGCTATGAA 
CGGGAAGTCA ATAAACTTAA AATCCAGATG AAAGCAATTG ATGACAATCA GGAAATGCCT 
CCAAATAAAA AGAAAAAAGA GAAGGAGCGA TGTACTGCCC TTCAGGACAA GCTTCTCGAA 
GAAGAAAAGA AACAGATGGA ACATGTACAG CGAGTTCTGC AGAGACTGAA ACTGGAAAAG 
GACAACTGGC TTTTAGCAAA ATCTACCAAA AATGAGACCA TCACAAAATT TCTACAGCTG 
TGTATATTTC CTCGATGTAT TTTTTCAGCA ATTGATGCTG TTTACTGTGC TCGTTTTGTT 
GAATTGGTAC ATCAACAGAA AACTCCAAAT TTTTCCACAC TTCTTTGCTA TGATCGAGTT 
TTCTCTGATA TAATTTACAC AGTTGCAAGC TGTACTGAAA ATGAAGCTAG TCGATATGGG 
AGATTCCTTT GCTGCATGTT AGAGACTGTG ACCAGGTGGC ACAGTGATAG AGCCACATAT 
GAAAAGGAAT GTGGAAATTA TCCAGGATTC CTTACTATAT TACGAGCAAC TGGATTTGAT 
GGTGGAAATA AAGCTGATCA ATTAGACTAT GAAAATTTTC GACATGTTGT ACACAAATGG 
CATTACAAAC TAACCAAGGC CTCGGTACAT TGCCTTGAAA CAGGCGAATA TACTCACATC 
AGGAATATCT TGATTGTGCT AACAAAAATA CTTCCTTGGT ACCCAAAAGT TTTGAATCTG 
GGTCAGGCAT TGGAAAGAAG AGTGCATAAA ATCTGCCAAG AGGAAAAAGA GAAGAGGCCA 
GATCTATATG CATTGGCTAT GGGCTACTCT GGGCAGTTGA AAAGTAGAAA GTCATACATG 
ATACCTGAAA ATGAGTTTCA TCACAAAGAC CCCCCTCCGA GGAATGCAGT TGCCAGTGTA 
CAAAATGGGC CTGGTGGTGG GCCTTCTTCA TCGTCGATCG GAAGTGTATC TAAGTCAGAC 
GAAAGCAGTA CCGAGGACAC TGATAAATCA AGGGAGAGAT CTCAGTGTGG TGTGAAAGCT 
GTTAATAAAG CTTCTAGTGC CACACCAAAA GGGAATTCAA GTAATGGAAA TAGTGGCTCT 
AACAGCAAAA CTGTTAAAGA AAATGACAAA GAAAAAGGAA AAGAGAAAGA AAAAGAGAAA 
AAAGAAAAGA CTCCAGCTAC TACTCCAGAG GCCCGAGTAC TTGGTAAAGA TGGTAAAGAA 
AAACCAAAGG AAGAACGGCC AAATAAAGAT GAAAAAGCAA GAGAGACAAA GGAAAGAACA 
CCTAAATCTG ACAAAGAGAA AGAAAAATTC AAGAAGGAAG AAAAAGCTAA AGATGAGAAA 
TTCAAGACCA CTGTCCCGAA TGTAGAATCA AAGTCAACTC AAGAAAAGGA AAGAGAGAAG 
GAGCCGTCCA GAGAACGAGA TATAGCAAAG GAAATGAAAT CAAAGGAAAA TGTTAAAGGA 
GGAGAGAAAA CACCAGTTTC TGGGTCCTTG AAATCACCTG TTCCCCGATC AGATATTACA 
GAGCCTGAAA GGGAACAGAA ACGTCGCAAA ATTGATACCC ATCCTTCTCC ATCACATTCC 
TCAACAGTAA AGGACAGTCT CATCGAACTC AAGGAGTCTT CAGCAAAGCT CTACATTAAT 
CATACTCCTC CACCACTGTC CAAGAGTAAG GAGAGAGAAA TGGACAAGAA AGATTTGGAC 
AAGTCAAGGG AAAGATCCAG AGAAAGAGAA AAAAAAGATG AAAAGGACAG GAAAGAGCGG 
AAAAGGGATC ACTCAAACAA TGACCGAGAA GTGCCACCGG ACTTAACCAA GAGGCGGAAA 
GAGGAAAATG GAACAATGGG GGTTTCAAAG CACAAAAGTG AAAGTCCATG TGAGTCTCCT 
TATCCAAATG AGAAAGACAA GGAAAAAAAT AAGTCAAAAT CTTCAGGCAA AGAAAAAGGC 
GGCGACTCAT TTAAATCTGA AAAGATGGAT AAAATCTCCT CTGGTGGCAA AAAGGAGTCC 
AGGCATGATA AAGAAAAGAT AGAAAAGAAA GAGAAACGGG ACAGTTCAGG AGGAAAGGAA 
GAGAAGAAAC ATCATAAGTC CTCGGACAAG CACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	THOC2
Entrez Gene ID	102401008
Full Name	THO complex 2
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

THOC2 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

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Target ORF information:

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