TTC21A cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	TTC21A
Entrez Gene ID	102399930
Full Name	tetratricopeptide repeat domain 21A
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006067367.1	XP_006067429.1	LOW QUALITY PROTEIN: tetratricopeptide repeat protein 21A
XM_025272322.1	XP_025128107.1	tetratricopeptide repeat protein 21A isoform X5
XM_025272319.1	XP_025128104.1	tetratricopeptide repeat protein 21A isoform X2
XM_025272320.1	XP_025128105.1	tetratricopeptide repeat protein 21A isoform X3
XM_025272321.1	XP_025128106.1	tetratricopeptide repeat protein 21A isoform X4
XM_025272317.1	XP_025128102.1	tetratricopeptide repeat protein 21A isoform X1
XM_025272318.1	XP_025128103.1	tetratricopeptide repeat protein 21A isoform X1

Pathways from BioSystems
Gene Ontology

The following TTC21A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTC21A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu22552	XM_006067367.1 Latest version!	Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu57654	XM_025272317.1 Latest version!	Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu57654	XM_025272318.1 Latest version!	Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu57655	XM_025272319.1 Latest version!	Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu57656	XM_025272320.1 Latest version!	Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu57657	XM_025272321.1 Latest version!	Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu57658	XM_025272322.1 Latest version!	Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTC21A ( XM_006067367.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006067429.1 Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), mRNA.

Price & Availability↑

CloneID	OBu22552
Clone ID Related Accession (Same CDS sequence)	XM_006067367.1
Accession Version	XM_006067367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	LOW QUALITY PROTEIN: tetratricopeptide repeat protein 21A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785254.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA ATGACTCCTC CCTTATGGCT GGGATCATTT ACTACAGCAA AGAAAAGTAC 
TTCCGCCACG TGCAGGAGGC TGCAGCTGTG GGCCTGGAAA AATTCAACAA TGACCCAGTG 
CTGCAGTTCT TTAAAGCCTA TGGAGTCCTC AGGGAAGAGC ACATCCAGGA TGCCATCAGC 
AGCCTGGAGA GCATCCGGAA TCACCCAGAT GTGTCCCTGT GCTCCATCAT GGCCCTCATT 
TATGCTCACA AGTGCTGTGA AACCATTGAC CGAGAAGCAA TTCAGGAGCT TGAGAGCAGC 
CTGAAAGAAG TCCGCAAGAC AGCCAGTGGG ACTGCCCTGT ACTATGCTGG CCTCTTCCTC 
TGGCTCATGG GCCTCCACGA CAAGGCCAAG GAATACATTG ACCGCACGTT GAAGATCTCC 
AGCAGCTCCA GAGAGGGCTA TGTGCTCAGA GGCTGGGTGG ACCTCAGCTC AGACAAACCC 
CACACGGTGA AGAAGTCCAT CAAGTACCTG GAGCATGGCA TTCAGGACAC CAAAGACGTC 
CTGGGACTAA TGGGAAAGGT GATGTACTTC ATGATGCTGC AGAACTACTC GGGGGCCCTT 
GAGGTGGTGA ACCAGATCAC TGTCACCTGC GGGAGCTTCC TGCCTGCCTT GGTCCTAAAG 
ATGCGGCTGT TCCTGGCTCG GCAGGACTGG GAGCAGACGG TAGAAATGGG ACACAGAATC 
CTCGAAAAAG ACAAAAGCAA TATCGATGCC TGCCAAATTC TAGCTGTGCA TGAGCTCGCA 
AGAGAAGGAA ACATGACCGT GGTAAGTTCT TTCAAGACTC AGAAGTTGAA CCTTGAAACC 
AGCCCAGCCA AGGAGGGACC CTTCACAGCT GCCGAGCACG TTAGGAATCT GATTAAGGCA 
CTGGAAACCA GAGAGCCCCA AAATCCAAGC CTCCATCTTA AAAAAATTCT TGTGGTGAGC 
AGACTGTGTG GGAGGCACCA GGGGATTCTG CAGCTGGTGT GTAGCTTCAT CGAACGAGCC 
TTCATGGCCA CATCCTCCGA CGCCCACATG GCCACCGAGC TGGGCTACCT CTTCATCCTG 
CAGGACCAAG TAAAGGAGGC GTCTCTGTGG TACTCGAAGG CCATGAAGCT GGACGAGAGC 
AGCGTGGCTG CTTTGACAGG GATCATCTGG TGCCAGATCT TAGACGGCCA CCTGGAGGAG 
GCCGAACACC AGCTGGAATT CCTGAAGGAG GTGCAGCAGT CCCTGGGGAA GTCCGAGCCC 
CGGTCTCCAG GCCAGATCGT GTCTCCTCTT CTTAAGCAAG TCACTGCGAT CTTGAATCCT 
GTAGTCAAAG TGGCACCAGC CCTGATTGAG CCGCTGTATG TGATGGCCCA GGTCAAGTAT 
CTCTCAGGGG AGTTAGAGCA TGCCCAGAGC ACCCTGCAGC GTTGCCTGGA GCTGGACCCC 
ACCTCCGTGG ACGCCCACCT CCTCATGGCT CAGATCTACC TGGCTCAGGA CAACTTTGCC 
ATGTGCTCCC ACTCCTTGGA GCTGGGCGTC AGCCACAACT TCCAGGTCCG AGACCACCCG 
CTTTACCACT TCATCAAGGC TAAGGCCCTC AACAAGTCTG GGGACTATCC AGAAGCTATA 
AAGGCACTGA AGATGATCAT AAAATTACCA ACTCTGAGGA CGGAAGAGAA CAAGAAGTTC 
CGTGGGCCCT GCGTGCGCCC CAGCGAGCGG GTGTCCATCT TACTGGAGCT GGCAGATGCC 
CTCCGGATGA ATGGAGAGCT GCACGAGGCC ACCAAGGTCA TGCAGGATGC CATCAACGAG 
TTCAGCGGCA CGCCGGAGGA GATGCGCATC ACCATCGCCA ATGTGGACTT GGCCCTGAGC 
AAGGGCAACG TGGACCTGGC TCTGAGCGTG CTGCAGAGCA TCACACCCAA GCAGCCCTGC 
TACATGGAAG CCAAGGAGAA GATGGCCAGC ATCTACCTGC AGACCCGCAA GGACGCCCGC 
CTCTATATCG GGTGCTACCG GGAGCTCTGT GAACATCTGC CTGGCCCCCA CACCAGTCTG 
CTTCTGGGTG ATGCTTTCAT GAACATTCAG GAGCCAGAGA AGGCCCTGGA GGTCTATGAT 
GAGGCCTACA GAAAGAACCC ACACGACACA TCCCTGATCA GCCGGATCGG GCAGGCTTAT 
GTCAAGACCC ACCAGTACAC CAAGGCAATT AATTATTATG AGGCTGCCCA GAAGATTAGC 
GGACAGGACT TTGTGTGCTG CGATCTGGCC GAACTTCTCC TGAAGTTAAA GAAGTTCAAC 
AAAGCAGAAA AAGTTTTGAA GCAGGCACTG GATCATGACT TTGTCAAAGA CATCCCATCC 
ATGATGAATG ATGTCAAGTG CTTACTTTTG CTGGCCAAGG TTTACAAGAG TCATAAAAAA 
GAAGATGTGA CGGAAACTTT GAACAAGGTA ATTGACAAAC AATGCCAGCA GCTCTCACGC 
ACTGAATGCT CTGTGCCGAG TTCTGCAGTG CAGTCTTATA AAGATGCCCT CTCCTACACG 
CCTATTAACA ATAAGGTGGT GCTGGAGCTG GCGAGGCTCT ACCTGCTGCA GGGACACCTG 
GACCTGTGTG AGCAGCACTG TGCCGACCTC CTGCAGACTG AGAAGAACCA CGAGGCAGCC 
TCCGTGGTGA TGGCTGACCT GATGTTTAGA AGACAGAAAT ACGAGGCTGC CATCAACCTC 
TACCACCAAG TCCTGGAGAA AGCACCAGAC AATTTTCCTG TATTGAACAA ACTAATCGAT 
CTGCTACGAA GAAGTGGCAA ACTTGAGGCC GCCCCTGCCG TCTTTGAATT GGCCGAGAAG 
ATGTCCAGCC GGGTGCCTTT GGAGCCAGGG TTCAACTTCT GCAGAGGCAT CTACTGCTGG 
CACATAGGAC AGCCCAATGA AGCCCTGAAG TTCCTGAACA AAGCGCGCAA GGACAGCGCT 
TGGGGCCAGA GCGCCACCTA CCACATGGTG CAGATCTGTC TGAACCCAGA CAACGAGATC 
GTGGGCGGAG AGGCTTTCGA GAACCTGGTG ACCGAGAGCA ACTTCAGCAG CAGGAAGGAG 
TTGGAGCAAC ATGGCGTGCG CACCGCCGAG AAGCTGCTGC AGGAGTTCTA CCCACACTCG 
GCCGGGGGCC ATACCCAGCT GCGGCTGCTG CAGAGCCTGT GCCTGCTGGC GACCAGGGAG 
AAGGCCAACG TGGAAGTGGC GCTGAGCACC TTCGTTGAGA TGGCCCAGGC TGAGAAGGAC 
AGCGTCCCCG CCCTGCTGGC CATGGCGCAG GCCTGCTCCC TGCTGAAGCA GGTCCCCAAG 
GCACGCACGC AGCTGAAGCG CCTGGCCAAG GCCCCGTGGG CACTGGCTGA GGCCGATGAC 
CTGGAGAGGA GCTGGCTCCT GCTGGCCGAT ATCTACTGCC AGGGCGGCAA GTTTGACCTG 
GCCTTGGAGC TGCTGCAGCG CTGCGTGAAA TACAACAAGT CCTGCTGTAA GGCCTACGAG 
TACATGGGCT TCATCATGGA AAGGGAGCGG TCATACAAGG ACGCGGCCTC CAACTACGAG 
CTGGCCTGGC AGTACAGCCG TCACACCAGC CCCGCCATCG GCTTCAGGCT CGCTTTCAAC 
TACTTGAAAG ACAAGAGATT CGTGGAAGCC ATTGAAGTCT GCCATGATGT CCTCAGGGAG 
CACCCCAACT ACCCTAAAAT CAGAGAAGAA ATTCTGGCAA AGGCCCAAGG GTCTCTGAGG 
CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006067429.1
CDS	105..3830
Translation	MSSNDSSLMAGIIYYSKEKYFRHVQEAAAVGLEKFNNDPVLQFFKAYGVLREEHIQDAISSLESIRNHPDVSLCSIMALIYAHKCCETIDREAIQELESSLKEVRKTASGTALYYAGLFLWLMGLHDKAKEYIDRTLKISSSSREGYVLRGWVDLSSDKPHTVKKSIKYLEHGIQDTKDVLGLMGKVMYFMMLQNYSGALEVVNQITVTCGSFLPALVLKMRLFLARQDWEQTVEMGHRILEKDKSNIDACQILAVHELAREGNMTVVSSFKTQKLNLETSPAKEGPFTAAEHVRNLIKALETREPQNPSLHLKKILVVSRLCGRHQGILQLVCSFIERAFMATSSDAHMATELGYLFILQDQVKEASLWYSKAMKLDESSVAALTGIIWCQILDGHLEEAEHQLEFLKEVQQSLGKSEPRSPGQIVSPLLKQVTAILNPVVKVAPALIEPLYVMAQVKYLSGELEHAQSTLQRCLELDPTSVDAHLLMAQIYLAQDNFAMCSHSLELGVSHNFQVRDHPLYHFIKAKALNKSGDYPEAIKALKMIIKLPTLRTEENKKFRGPCVRPSERVSILLELADALRMNGELHEATKVMQDAINEFSGTPEEMRITIANVDLALSKGNVDLALSVLQSITPKQPCYMEAKEKMASIYLQTRKDARLYIGCYRELCEHLPGPHTSLLLGDAFMNIQEPEKALEVYDEAYRKNPHDTSLISRIGQAYVKTHQYTKAINYYEAAQKISGQDFVCCDLAELLLKLKKFNKAEKVLKQALDHDFVKDIPSMMNDVKCLLLLAKVYKSHKKEDVTETLNKVIDKQCQQLSRTECSVPSSAVQSYKDALSYTPINNKVVLELARLYLLQGHLDLCEQHCADLLQTEKNHEAASVVMADLMFRRQKYEAAINLYHQVLEKAPDNFPVLNKLIDLLRRSGKLEAAPAVFELAEKMSSRVPLEPGFNFCRGIYCWHIGQPNEALKFLNKARKDSAWGQSATYHMVQICLNPDNEIVGGEAFENLVTESNFSSRKELEQHGVRTAEKLLQEFYPHSAGGHTQLRLLQSLCLLATREKANVEVALSTFVEMAQAEKDSVPALLAMAQACSLLKQVPKARTQLKRLAKAPWALAEADDLERSWLLLADIYCQGGKFDLALELLQRCVKYNKSCCKAYEYMGFIMERERSYKDAASNYELAWQYSRHTSPAIGFRLAFNYLKDKRFVEAIEVCHDVLREHPNYPKIREEILAKAQGSLRP

Target ORF information:

RefSeq Version	XM_006067367.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006067367.1

ORF Insert Sequence:

ATGAGCAGCA ATGACTCCTC CCTTATGGCT GGGATCATTT ACTACAGCAA AGAAAAGTAC 
TTCCGCCACG TGCAGGAGGC TGCAGCTGTG GGCCTGGAAA AATTCAACAA TGACCCAGTG 
CTGCAGTTCT TTAAAGCCTA TGGAGTCCTC AGGGAAGAGC ACATCCAGGA TGCCATCAGC 
AGCCTGGAGA GCATCCGGAA TCACCCAGAT GTGTCCCTGT GCTCCATCAT GGCCCTCATT 
TATGCTCACA AGTGCTGTGA AACCATTGAC CGAGAAGCAA TTCAGGAGCT TGAGAGCAGC 
CTGAAAGAAG TCCGCAAGAC AGCCAGTGGG ACTGCCCTGT ACTATGCTGG CCTCTTCCTC 
TGGCTCATGG GCCTCCACGA CAAGGCCAAG GAATACATTG ACCGCACGTT GAAGATCTCC 
AGCAGCTCCA GAGAGGGCTA TGTGCTCAGA GGCTGGGTGG ACCTCAGCTC AGACAAACCC 
CACACGGTGA AGAAGTCCAT CAAGTACCTG GAGCATGGCA TTCAGGACAC CAAAGACGTC 
CTGGGACTAA TGGGAAAGGT GATGTACTTC ATGATGCTGC AGAACTACTC GGGGGCCCTT 
GAGGTGGTGA ACCAGATCAC TGTCACCTGC GGGAGCTTCC TGCCTGCCTT GGTCCTAAAG 
ATGCGGCTGT TCCTGGCTCG GCAGGACTGG GAGCAGACGG TAGAAATGGG ACACAGAATC 
CTCGAAAAAG ACAAAAGCAA TATCGATGCC TGCCAAATTC TAGCTGTGCA TGAGCTCGCA 
AGAGAAGGAA ACATGACCGT GGTAAGTTCT TTCAAGACTC AGAAGTTGAA CCTTGAAACC 
AGCCCAGCCA AGGAGGGACC CTTCACAGCT GCCGAGCACG TTAGGAATCT GATTAAGGCA 
CTGGAAACCA GAGAGCCCCA AAATCCAAGC CTCCATCTTA AAAAAATTCT TGTGGTGAGC 
AGACTGTGTG GGAGGCACCA GGGGATTCTG CAGCTGGTGT GTAGCTTCAT CGAACGAGCC 
TTCATGGCCA CATCCTCCGA CGCCCACATG GCCACCGAGC TGGGCTACCT CTTCATCCTG 
CAGGACCAAG TAAAGGAGGC GTCTCTGTGG TACTCGAAGG CCATGAAGCT GGACGAGAGC 
AGCGTGGCTG CTTTGACAGG GATCATCTGG TGCCAGATCT TAGACGGCCA CCTGGAGGAG 
GCCGAACACC AGCTGGAATT CCTGAAGGAG GTGCAGCAGT CCCTGGGGAA GTCCGAGCCC 
CGGTCTCCAG GCCAGATCGT GTCTCCTCTT CTTAAGCAAG TCACTGCGAT CTTGAATCCT 
GTAGTCAAAG TGGCACCAGC CCTGATTGAG CCGCTGTATG TGATGGCCCA GGTCAAGTAT 
CTCTCAGGGG AGTTAGAGCA TGCCCAGAGC ACCCTGCAGC GTTGCCTGGA GCTGGACCCC 
ACCTCCGTGG ACGCCCACCT CCTCATGGCT CAGATCTACC TGGCTCAGGA CAACTTTGCC 
ATGTGCTCCC ACTCCTTGGA GCTGGGCGTC AGCCACAACT TCCAGGTCCG AGACCACCCG 
CTTTACCACT TCATCAAGGC TAAGGCCCTC AACAAGTCTG GGGACTATCC AGAAGCTATA 
AAGGCACTGA AGATGATCAT AAAATTACCA ACTCTGAGGA CGGAAGAGAA CAAGAAGTTC 
CGTGGGCCCT GCGTGCGCCC CAGCGAGCGG GTGTCCATCT TACTGGAGCT GGCAGATGCC 
CTCCGGATGA ATGGAGAGCT GCACGAGGCC ACCAAGGTCA TGCAGGATGC CATCAACGAG 
TTCAGCGGCA CGCCGGAGGA GATGCGCATC ACCATCGCCA ATGTGGACTT GGCCCTGAGC 
AAGGGCAACG TGGACCTGGC TCTGAGCGTG CTGCAGAGCA TCACACCCAA GCAGCCCTGC 
TACATGGAAG CCAAGGAGAA GATGGCCAGC ATCTACCTGC AGACCCGCAA GGACGCCCGC 
CTCTATATCG GGTGCTACCG GGAGCTCTGT GAACATCTGC CTGGCCCCCA CACCAGTCTG 
CTTCTGGGTG ATGCTTTCAT GAACATTCAG GAGCCAGAGA AGGCCCTGGA GGTCTATGAT 
GAGGCCTACA GAAAGAACCC ACACGACACA TCCCTGATCA GCCGGATCGG GCAGGCTTAT 
GTCAAGACCC ACCAGTACAC CAAGGCAATT AATTATTATG AGGCTGCCCA GAAGATTAGC 
GGACAGGACT TTGTGTGCTG CGATCTGGCC GAACTTCTCC TGAAGTTAAA GAAGTTCAAC 
AAAGCAGAAA AAGTTTTGAA GCAGGCACTG GATCATGACT TTGTCAAAGA CATCCCATCC 
ATGATGAATG ATGTCAAGTG CTTACTTTTG CTGGCCAAGG TTTACAAGAG TCATAAAAAA 
GAAGATGTGA CGGAAACTTT GAACAAGGTA ATTGACAAAC AATGCCAGCA GCTCTCACGC 
ACTGAATGCT CTGTGCCGAG TTCTGCAGTG CAGTCTTATA AAGATGCCCT CTCCTACACG 
CCTATTAACA ATAAGGTGGT GCTGGAGCTG GCGAGGCTCT ACCTGCTGCA GGGACACCTG 
GACCTGTGTG AGCAGCACTG TGCCGACCTC CTGCAGACTG AGAAGAACCA CGAGGCAGCC 
TCCGTGGTGA TGGCTGACCT GATGTTTAGA AGACAGAAAT ACGAGGCTGC CATCAACCTC 
TACCACCAAG TCCTGGAGAA AGCACCAGAC AATTTTCCTG TATTGAACAA ACTAATCGAT 
CTGCTACGAA GAAGTGGCAA ACTTGAGGCC GCCCCTGCCG TCTTTGAATT GGCCGAGAAG 
ATGTCCAGCC GGGTGCCTTT GGAGCCAGGG TTCAACTTCT GCAGAGGCAT CTACTGCTGG 
CACATAGGAC AGCCCAATGA AGCCCTGAAG TTCCTGAACA AAGCGCGCAA GGACAGCGCT 
TGGGGCCAGA GCGCCACCTA CCACATGGTG CAGATCTGTC TGAACCCAGA CAACGAGATC 
GTGGGCGGAG AGGCTTTCGA GAACCTGGTG ACCGAGAGCA ACTTCAGCAG CAGGAAGGAG 
TTGGAGCAAC ATGGCGTGCG CACCGCCGAG AAGCTGCTGC AGGAGTTCTA CCCACACTCG 
GCCGGGGGCC ATACCCAGCT GCGGCTGCTG CAGAGCCTGT GCCTGCTGGC GACCAGGGAG 
AAGGCCAACG TGGAAGTGGC GCTGAGCACC TTCGTTGAGA TGGCCCAGGC TGAGAAGGAC 
AGCGTCCCCG CCCTGCTGGC CATGGCGCAG GCCTGCTCCC TGCTGAAGCA GGTCCCCAAG 
GCACGCACGC AGCTGAAGCG CCTGGCCAAG GCCCCGTGGG CACTGGCTGA GGCCGATGAC 
CTGGAGAGGA GCTGGCTCCT GCTGGCCGAT ATCTACTGCC AGGGCGGCAA GTTTGACCTG 
GCCTTGGAGC TGCTGCAGCG CTGCGTGAAA TACAACAAGT CCTGCTGTAA GGCCTACGAG 
TACATGGGCT TCATCATGGA AAGGGAGCGG TCATACAAGG ACGCGGCCTC CAACTACGAG 
CTGGCCTGGC AGTACAGCCG TCACACCAGC CCCGCCATCG GCTTCAGGCT CGCTTTCAAC 
TACTTGAAAG ACAAGAGATT CGTGGAAGCC ATTGAAGTCT GCCATGATGT CCTCAGGGAG 
CACCCCAACT ACCCTAAAAT CAGAGAAGAA ATTCTGGCAA AGGCCCAAGG GTCTCTGAGG 
CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC21A ( XM_025272317.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128102.1 Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu57654
Clone ID Related Accession (Same CDS sequence)	XM_025272317.1 , XM_025272318.1
Accession Version	XM_025272317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	tetratricopeptide repeat protein 21A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA ATGACTCCTC CCTTATGGCT GGGATCATTT ACTACAGCAA AGAAAAGTAC 
TTCCGCCACG TGCAGGAGGC TGCAGCTGTG GGCCTGGAAA AATTCAACAA TGACCCAGTG 
CTGCAGTTCT TTAAAGCCTA TGGAGTCCTC AGGGAAGAGC ACATCCAGGA TGCCATCAGC 
AGCCTGGAGA GCATCCGGAA TCACCCAGAT GTGTCCCTGT GCTCCATCAT GGCCCTCATT 
TATGCTCACA AGTGCTGTGA AACCATTGAC CGAGAAGCAA TTCAGGAGCT TGAGAGCAGC 
CTGAAAGAAG TCCGCAAGAC AGCCAGTGGG ACTGCCCTGT ACTATGCTGG CCTCTTCCTC 
TGGCTCATGG GCCTCCACGA CAAGGCCAAG GAATACATTG ACCGCACGTT GAAGATCTCC 
AGCAGCTCCA GAGAGGGCTA TGTGCTCAGA GGCTGGGTGG ACCTCAGCTC AGACAAACCC 
CACACGGTGA AGAAGTCCAT CAAGTACCTG GAGCATGGCA TTCAGGACAC CAAAGACGTC 
CTGGGACTAA TGGGAAAGGT GATGTACTTC ATGATGCTGC AGAACTACTC GGGGGCCCTT 
GAGGTGGTGA ACCAGATCAC TGTCACCTGC GGGAGCTTCC TGCCTGCCTT GGTCCTAAAG 
ATGCGGCTGT TCCTGGCTCG GCAGGACTGG GAGCAGACGG TAGAAATGGG ACACAGAATC 
CTCGAAAAAG ACAAAAGCAA TATCGATGCC TGCCAAATTC TAGCTGTGCA TGAGCTCGCA 
AGAGAAGGAA ACATGACCGT GGCTGCCGAG CACGTTAGGA ATCTGATTAA GGCACTGGAA 
ACCAGAGAGC CCCAAAATCC AAGCCTCCAT CTTAAAAAAA TTCTTGTGGT GAGCAGACTG 
TGTGGGAGGC ACCAGGGGAT TCTGCAGCTG GTGTGTAGCT TCATCGAACG AGCCTTCATG 
GCCACATCCT CCGACGCCCA CATGGCCACC GAGCTGGGCT ACCTCTTCAT CCTGCAGGAC 
CAAGTAAAGG AGGCGTCTCT GTGGTACTCG AAGGCCATGA AGCTGGACGA GAGCAGCGTG 
GCTGCTTTGA CAGGGATCAT CTGGTGCCAG ATCTTAGACG GCCACCTGGA GGAGGCCGAA 
CACCAGCTGG AATTCCTGAA GGAGGTGCAG CAGTCCCTGG GGAAGTCCGA GGTGCTGGTT 
TTCCTGCAAG CCCTCCTGGC GTCCAAGAAG CATAAGGGGG AGCAGGAGGC CACGACGCTG 
CTAAAGGAGG CCGCTGAGCT GCACTTCTCC AGCATGCAGG CCCTCCCCAT GAGCTCCGAA 
TACTTGGAAC GGCTCGACCC CATCTTCCTG GTCTGCATCG CCAAGGAATA CCTGGTCTTC 
TGCCCCAAGC AGCCCCGGTC TCCAGGCCAG ATCGTGTCTC CTCTTCTTAA GCAAGTCACT 
GCGATCTTGA ATCCTGTAGT CAAAGTGGCA CCAGCCCTGA TTGAGCCGCT GTATGTGATG 
GCCCAGGTCA AGTATCTCTC AGGGGAGTTA GAGCATGCCC AGAGCACCCT GCAGCGTTGC 
CTGGAGCTGG ACCCCACCTC CGTGGACGCC CACCTCCTCA TGGCTCAGAT CTACCTGGCT 
CAGGACAACT TTGCCATGTG CTCCCACTCC TTGGAGCTGG GCGTCAGCCA CAACTTCCAG 
GTCCGAGACC ACCCGCTTTA CCACTTCATC AAGGCCAAGG CCCTCAACAA GTCTGGGGAC 
TATCCAGAAG CTATAAAGGC ACTGAAGATG ATCATAAAAT TACCAACTCT GAGGACGGAA 
GAGAACAAGA AGTTCCGTGG GCCCTGCGTG CGCCCCAGCG AGCGGGTGTC CATCTTACTG 
GAGCTGGCAG ATGCCCTCCG GATGAATGGA GAGCTGCACG AGGCCACCAA GGTCATGCAG 
GATGCCATCA ACGAGTTCAG CGGCACGCCG GAGGAGATGC GCATCACCAT CGCCAATGTG 
GACTTGGCCC TGAGCAAGGG CAACGTGGAC CTGGCTCTGA GCGTGCTGCA GAGCATCACA 
CCCAAGCAGC CCTGCTACAT GGAAGCCAAG GAGAAGATGG CCAGCATCTA CCTGCAGACC 
CGCAAGGACG CCCGCCTCTA TATCGGGTGC TACCGGGAGC TCTGTGAACA TCTGCCTGGC 
CCCCACACCA GTCTGCTTCT GGGTGATGCT TTCATGAACA TTCAGGAGCC AGAGAAGGCC 
CTGGAGGTCT ATGATGAGGC CTACAGAAAG AACCCACACG ACACATCCCT GATCAGCCGG 
ATCGGGCAGG CTTATGTCAA GACCCACCAG TACACCAAGG CAATTAATTA TTATGAGGCT 
GCCCAGAAGA TTAGCGGACA GGACTTTGTG TGCTGCGATC TGGCCGAACT TCTCCTGAAG 
TTAAAGAAGT TCAACAAAGC AGAAAAAGTT TTGAAGCAGG CACTGGATCA TGACTTTGTC 
AAAGACATCC CATCCATGAT GAATGATGTC AAGTGCTTAC TTTTGCTGGC CAAGGTTTAC 
AAGAGTCATA AAAAAGAAGA TGTGACGGAA ACTTTGAACA AGGCCATGGA CCTCCAGTCT 
CGGATCCTGA AGCGTGTTCC CCTGGAGCGA CCAGAAATGA TCCCCTCCCA GAAGCAACTG 
GCAGCCTCCA TCTGCATCCA GTTTGGGGAA TACTACCTGG CAGAGAAGGA GTATGCCAAG 
GCAGTGCAGT CTTATAAAGA TGCCCTCTCC TACACGCCTA TTAACAATAA GGTGGTGCTG 
GAGCTGGCGA GGCTCTACCT GCTGCAGGGA CACCTGGACC TGTGTGAGCA GCACTGTGCC 
GACCTCCTGC AGACTGAGAA GAACCACGAG GCAGCCTCCG TGGTGATGGC TGACCTGATG 
TTTAGAAGAC AGAAATACGA GGCTGCCATC AACCTCTACC ACCAAGTCCT GGAGAAAGCA 
CCAGACAATT TTCCTGTATT GAACAAACTA ATCGATCTGC TACGAAGAAG TGGCAAACTT 
GAGGCCGCCC CTGCCGTCTT TGAATTGGCC GAGAAGATGT CCAGCCGGGT GCCTTTGGAG 
CCAGGGTTCA ACTTCTGCAG AGGCATCTAC TGCTGGCACA TAGGACAGCC CAATGAAGCC 
CTGAAGTTCC TGAACAAAGC GCGCAAGGAC AGCGCTTGGG GCCAGAGCGC CACCTACCAC 
ATGGTGCAGA TCTGTCTGAA CCCAGACAAC GAGATCGTGG GCGGAGAGGC TTTCGAGAAC 
CTGGTGACCG AGAGCAACCC CAGTGCCCGC AGCTTCAGCA GCAGGAAGGA GTTGGAGCAA 
CATGGCGTGC GCACCGCCGA GAAGCTGCTG CAGGAGTTCT ACCCACACTC GGCCGGGGGC 
CATACCCAGC TGCGGCTGCT GCAGAGCCTG TGCCTGCTGG CGACCAGGGA GAAGGCCAAC 
GTGGAAGTGG CGCTGAGCAC CTTCGTTGAG ATGGCCCAGG CTGAGAAGGA CAGCGTCCCC 
GCCCTGCTGG CCATGGCGCA GGCCTGCTCC CTGCTGAAGC AGGTCCCCAA GGCACGCACG 
CAGCTGAAGC GCCTGGCCAA GGCCCCGTGG GCACTGGCTG AGGCCGATGA CCTGGAGAGG 
AGCTGGCTCC TGCTGGCCGA TATCTACTGC CAGGGCGGCA AGTTTGACCT GGCCTTGGAG 
CTGCTGCAGC GCTGCGTGAA ATACAACAAG TCCTGCTGTA AGGCCTACGA GTACATGGGC 
TTCATCATGG AAAGGGAGCG GTCATACAAG GACGCGGCCT CCAACTACGA GCTGGCCTGG 
CAGTACAGCC GTCACACCAG CCCCGCCATC GGCTTCAGGC TCGCTTTCAA CTACTTGAAA 
GACAAGAGAT TCGTGGAAGC CATTGAAGTC TGCCATGATG TCCTCAGGGA GCACCCCAAC 
TACCCTAAAA TCAGAGAAGA AATTCTGGCA AAGGCCCAAG GGTCTCTGAG GCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128102.1
CDS	131..4087
Translation	MSSNDSSLMAGIIYYSKEKYFRHVQEAAAVGLEKFNNDPVLQFFKAYGVLREEHIQDAISSLESIRNHPDVSLCSIMALIYAHKCCETIDREAIQELESSLKEVRKTASGTALYYAGLFLWLMGLHDKAKEYIDRTLKISSSSREGYVLRGWVDLSSDKPHTVKKSIKYLEHGIQDTKDVLGLMGKVMYFMMLQNYSGALEVVNQITVTCGSFLPALVLKMRLFLARQDWEQTVEMGHRILEKDKSNIDACQILAVHELAREGNMTVAAEHVRNLIKALETREPQNPSLHLKKILVVSRLCGRHQGILQLVCSFIERAFMATSSDAHMATELGYLFILQDQVKEASLWYSKAMKLDESSVAALTGIIWCQILDGHLEEAEHQLEFLKEVQQSLGKSEVLVFLQALLASKKHKGEQEATTLLKEAAELHFSSMQALPMSSEYLERLDPIFLVCIAKEYLVFCPKQPRSPGQIVSPLLKQVTAILNPVVKVAPALIEPLYVMAQVKYLSGELEHAQSTLQRCLELDPTSVDAHLLMAQIYLAQDNFAMCSHSLELGVSHNFQVRDHPLYHFIKAKALNKSGDYPEAIKALKMIIKLPTLRTEENKKFRGPCVRPSERVSILLELADALRMNGELHEATKVMQDAINEFSGTPEEMRITIANVDLALSKGNVDLALSVLQSITPKQPCYMEAKEKMASIYLQTRKDARLYIGCYRELCEHLPGPHTSLLLGDAFMNIQEPEKALEVYDEAYRKNPHDTSLISRIGQAYVKTHQYTKAINYYEAAQKISGQDFVCCDLAELLLKLKKFNKAEKVLKQALDHDFVKDIPSMMNDVKCLLLLAKVYKSHKKEDVTETLNKAMDLQSRILKRVPLERPEMIPSQKQLAASICIQFGEYYLAEKEYAKAVQSYKDALSYTPINNKVVLELARLYLLQGHLDLCEQHCADLLQTEKNHEAASVVMADLMFRRQKYEAAINLYHQVLEKAPDNFPVLNKLIDLLRRSGKLEAAPAVFELAEKMSSRVPLEPGFNFCRGIYCWHIGQPNEALKFLNKARKDSAWGQSATYHMVQICLNPDNEIVGGEAFENLVTESNPSARSFSSRKELEQHGVRTAEKLLQEFYPHSAGGHTQLRLLQSLCLLATREKANVEVALSTFVEMAQAEKDSVPALLAMAQACSLLKQVPKARTQLKRLAKAPWALAEADDLERSWLLLADIYCQGGKFDLALELLQRCVKYNKSCCKAYEYMGFIMERERSYKDAASNYELAWQYSRHTSPAIGFRLAFNYLKDKRFVEAIEVCHDVLREHPNYPKIREEILAKAQGSLRP

Target ORF information:

RefSeq Version	XM_025272317.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025272317.1

ORF Insert Sequence:

ATGAGCAGCA ATGACTCCTC CCTTATGGCT GGGATCATTT ACTACAGCAA AGAAAAGTAC 
TTCCGCCACG TGCAGGAGGC TGCAGCTGTG GGCCTGGAAA AATTCAACAA TGACCCAGTG 
CTGCAGTTCT TTAAAGCCTA TGGAGTCCTC AGGGAAGAGC ACATCCAGGA TGCCATCAGC 
AGCCTGGAGA GCATCCGGAA TCACCCAGAT GTGTCCCTGT GCTCCATCAT GGCCCTCATT 
TATGCTCACA AGTGCTGTGA AACCATTGAC CGAGAAGCAA TTCAGGAGCT TGAGAGCAGC 
CTGAAAGAAG TCCGCAAGAC AGCCAGTGGG ACTGCCCTGT ACTATGCTGG CCTCTTCCTC 
TGGCTCATGG GCCTCCACGA CAAGGCCAAG GAATACATTG ACCGCACGTT GAAGATCTCC 
AGCAGCTCCA GAGAGGGCTA TGTGCTCAGA GGCTGGGTGG ACCTCAGCTC AGACAAACCC 
CACACGGTGA AGAAGTCCAT CAAGTACCTG GAGCATGGCA TTCAGGACAC CAAAGACGTC 
CTGGGACTAA TGGGAAAGGT GATGTACTTC ATGATGCTGC AGAACTACTC GGGGGCCCTT 
GAGGTGGTGA ACCAGATCAC TGTCACCTGC GGGAGCTTCC TGCCTGCCTT GGTCCTAAAG 
ATGCGGCTGT TCCTGGCTCG GCAGGACTGG GAGCAGACGG TAGAAATGGG ACACAGAATC 
CTCGAAAAAG ACAAAAGCAA TATCGATGCC TGCCAAATTC TAGCTGTGCA TGAGCTCGCA 
AGAGAAGGAA ACATGACCGT GGCTGCCGAG CACGTTAGGA ATCTGATTAA GGCACTGGAA 
ACCAGAGAGC CCCAAAATCC AAGCCTCCAT CTTAAAAAAA TTCTTGTGGT GAGCAGACTG 
TGTGGGAGGC ACCAGGGGAT TCTGCAGCTG GTGTGTAGCT TCATCGAACG AGCCTTCATG 
GCCACATCCT CCGACGCCCA CATGGCCACC GAGCTGGGCT ACCTCTTCAT CCTGCAGGAC 
CAAGTAAAGG AGGCGTCTCT GTGGTACTCG AAGGCCATGA AGCTGGACGA GAGCAGCGTG 
GCTGCTTTGA CAGGGATCAT CTGGTGCCAG ATCTTAGACG GCCACCTGGA GGAGGCCGAA 
CACCAGCTGG AATTCCTGAA GGAGGTGCAG CAGTCCCTGG GGAAGTCCGA GGTGCTGGTT 
TTCCTGCAAG CCCTCCTGGC GTCCAAGAAG CATAAGGGGG AGCAGGAGGC CACGACGCTG 
CTAAAGGAGG CCGCTGAGCT GCACTTCTCC AGCATGCAGG CCCTCCCCAT GAGCTCCGAA 
TACTTGGAAC GGCTCGACCC CATCTTCCTG GTCTGCATCG CCAAGGAATA CCTGGTCTTC 
TGCCCCAAGC AGCCCCGGTC TCCAGGCCAG ATCGTGTCTC CTCTTCTTAA GCAAGTCACT 
GCGATCTTGA ATCCTGTAGT CAAAGTGGCA CCAGCCCTGA TTGAGCCGCT GTATGTGATG 
GCCCAGGTCA AGTATCTCTC AGGGGAGTTA GAGCATGCCC AGAGCACCCT GCAGCGTTGC 
CTGGAGCTGG ACCCCACCTC CGTGGACGCC CACCTCCTCA TGGCTCAGAT CTACCTGGCT 
CAGGACAACT TTGCCATGTG CTCCCACTCC TTGGAGCTGG GCGTCAGCCA CAACTTCCAG 
GTCCGAGACC ACCCGCTTTA CCACTTCATC AAGGCCAAGG CCCTCAACAA GTCTGGGGAC 
TATCCAGAAG CTATAAAGGC ACTGAAGATG ATCATAAAAT TACCAACTCT GAGGACGGAA 
GAGAACAAGA AGTTCCGTGG GCCCTGCGTG CGCCCCAGCG AGCGGGTGTC CATCTTACTG 
GAGCTGGCAG ATGCCCTCCG GATGAATGGA GAGCTGCACG AGGCCACCAA GGTCATGCAG 
GATGCCATCA ACGAGTTCAG CGGCACGCCG GAGGAGATGC GCATCACCAT CGCCAATGTG 
GACTTGGCCC TGAGCAAGGG CAACGTGGAC CTGGCTCTGA GCGTGCTGCA GAGCATCACA 
CCCAAGCAGC CCTGCTACAT GGAAGCCAAG GAGAAGATGG CCAGCATCTA CCTGCAGACC 
CGCAAGGACG CCCGCCTCTA TATCGGGTGC TACCGGGAGC TCTGTGAACA TCTGCCTGGC 
CCCCACACCA GTCTGCTTCT GGGTGATGCT TTCATGAACA TTCAGGAGCC AGAGAAGGCC 
CTGGAGGTCT ATGATGAGGC CTACAGAAAG AACCCACACG ACACATCCCT GATCAGCCGG 
ATCGGGCAGG CTTATGTCAA GACCCACCAG TACACCAAGG CAATTAATTA TTATGAGGCT 
GCCCAGAAGA TTAGCGGACA GGACTTTGTG TGCTGCGATC TGGCCGAACT TCTCCTGAAG 
TTAAAGAAGT TCAACAAAGC AGAAAAAGTT TTGAAGCAGG CACTGGATCA TGACTTTGTC 
AAAGACATCC CATCCATGAT GAATGATGTC AAGTGCTTAC TTTTGCTGGC CAAGGTTTAC 
AAGAGTCATA AAAAAGAAGA TGTGACGGAA ACTTTGAACA AGGCCATGGA CCTCCAGTCT 
CGGATCCTGA AGCGTGTTCC CCTGGAGCGA CCAGAAATGA TCCCCTCCCA GAAGCAACTG 
GCAGCCTCCA TCTGCATCCA GTTTGGGGAA TACTACCTGG CAGAGAAGGA GTATGCCAAG 
GCAGTGCAGT CTTATAAAGA TGCCCTCTCC TACACGCCTA TTAACAATAA GGTGGTGCTG 
GAGCTGGCGA GGCTCTACCT GCTGCAGGGA CACCTGGACC TGTGTGAGCA GCACTGTGCC 
GACCTCCTGC AGACTGAGAA GAACCACGAG GCAGCCTCCG TGGTGATGGC TGACCTGATG 
TTTAGAAGAC AGAAATACGA GGCTGCCATC AACCTCTACC ACCAAGTCCT GGAGAAAGCA 
CCAGACAATT TTCCTGTATT GAACAAACTA ATCGATCTGC TACGAAGAAG TGGCAAACTT 
GAGGCCGCCC CTGCCGTCTT TGAATTGGCC GAGAAGATGT CCAGCCGGGT GCCTTTGGAG 
CCAGGGTTCA ACTTCTGCAG AGGCATCTAC TGCTGGCACA TAGGACAGCC CAATGAAGCC 
CTGAAGTTCC TGAACAAAGC GCGCAAGGAC AGCGCTTGGG GCCAGAGCGC CACCTACCAC 
ATGGTGCAGA TCTGTCTGAA CCCAGACAAC GAGATCGTGG GCGGAGAGGC TTTCGAGAAC 
CTGGTGACCG AGAGCAACCC CAGTGCCCGC AGCTTCAGCA GCAGGAAGGA GTTGGAGCAA 
CATGGCGTGC GCACCGCCGA GAAGCTGCTG CAGGAGTTCT ACCCACACTC GGCCGGGGGC 
CATACCCAGC TGCGGCTGCT GCAGAGCCTG TGCCTGCTGG CGACCAGGGA GAAGGCCAAC 
GTGGAAGTGG CGCTGAGCAC CTTCGTTGAG ATGGCCCAGG CTGAGAAGGA CAGCGTCCCC 
GCCCTGCTGG CCATGGCGCA GGCCTGCTCC CTGCTGAAGC AGGTCCCCAA GGCACGCACG 
CAGCTGAAGC GCCTGGCCAA GGCCCCGTGG GCACTGGCTG AGGCCGATGA CCTGGAGAGG 
AGCTGGCTCC TGCTGGCCGA TATCTACTGC CAGGGCGGCA AGTTTGACCT GGCCTTGGAG 
CTGCTGCAGC GCTGCGTGAA ATACAACAAG TCCTGCTGTA AGGCCTACGA GTACATGGGC 
TTCATCATGG AAAGGGAGCG GTCATACAAG GACGCGGCCT CCAACTACGA GCTGGCCTGG 
CAGTACAGCC GTCACACCAG CCCCGCCATC GGCTTCAGGC TCGCTTTCAA CTACTTGAAA 
GACAAGAGAT TCGTGGAAGC CATTGAAGTC TGCCATGATG TCCTCAGGGA GCACCCCAAC 
TACCCTAAAA TCAGAGAAGA AATTCTGGCA AAGGCCCAAG GGTCTCTGAG GCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC21A ( XM_025272318.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128103.1 Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X3, mRNA.

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CloneID	OBu57654
Clone ID Related Accession (Same CDS sequence)	XM_025272317.1 , XM_025272318.1
Accession Version	XM_025272318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	tetratricopeptide repeat protein 21A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA ATGACTCCTC CCTTATGGCT GGGATCATTT ACTACAGCAA AGAAAAGTAC 
TTCCGCCACG TGCAGGAGGC TGCAGCTGTG GGCCTGGAAA AATTCAACAA TGACCCAGTG 
CTGCAGTTCT TTAAAGCCTA TGGAGTCCTC AGGGAAGAGC ACATCCAGGA TGCCATCAGC 
AGCCTGGAGA GCATCCGGAA TCACCCAGAT GTGTCCCTGT GCTCCATCAT GGCCCTCATT 
TATGCTCACA AGTGCTGTGA AACCATTGAC CGAGAAGCAA TTCAGGAGCT TGAGAGCAGC 
CTGAAAGAAG TCCGCAAGAC AGCCAGTGGG ACTGCCCTGT ACTATGCTGG CCTCTTCCTC 
TGGCTCATGG GCCTCCACGA CAAGGCCAAG GAATACATTG ACCGCACGTT GAAGATCTCC 
AGCAGCTCCA GAGAGGGCTA TGTGCTCAGA GGCTGGGTGG ACCTCAGCTC AGACAAACCC 
CACACGGTGA AGAAGTCCAT CAAGTACCTG GAGCATGGCA TTCAGGACAC CAAAGACGTC 
CTGGGACTAA TGGGAAAGGT GATGTACTTC ATGATGCTGC AGAACTACTC GGGGGCCCTT 
GAGGTGGTGA ACCAGATCAC TGTCACCTGC GGGAGCTTCC TGCCTGCCTT GGTCCTAAAG 
ATGCGGCTGT TCCTGGCTCG GCAGGACTGG GAGCAGACGG TAGAAATGGG ACACAGAATC 
CTCGAAAAAG ACAAAAGCAA TATCGATGCC TGCCAAATTC TAGCTGTGCA TGAGCTCGCA 
AGAGAAGGAA ACATGACCGT GGCTGCCGAG CACGTTAGGA ATCTGATTAA GGCACTGGAA 
ACCAGAGAGC CCCAAAATCC AAGCCTCCAT CTTAAAAAAA TTCTTGTGGT GAGCAGACTG 
TGTGGGAGGC ACCAGGGGAT TCTGCAGCTG GTGTGTAGCT TCATCGAACG AGCCTTCATG 
GCCACATCCT CCGACGCCCA CATGGCCACC GAGCTGGGCT ACCTCTTCAT CCTGCAGGAC 
CAAGTAAAGG AGGCGTCTCT GTGGTACTCG AAGGCCATGA AGCTGGACGA GAGCAGCGTG 
GCTGCTTTGA CAGGGATCAT CTGGTGCCAG ATCTTAGACG GCCACCTGGA GGAGGCCGAA 
CACCAGCTGG AATTCCTGAA GGAGGTGCAG CAGTCCCTGG GGAAGTCCGA GGTGCTGGTT 
TTCCTGCAAG CCCTCCTGGC GTCCAAGAAG CATAAGGGGG AGCAGGAGGC CACGACGCTG 
CTAAAGGAGG CCGCTGAGCT GCACTTCTCC AGCATGCAGG CCCTCCCCAT GAGCTCCGAA 
TACTTGGAAC GGCTCGACCC CATCTTCCTG GTCTGCATCG CCAAGGAATA CCTGGTCTTC 
TGCCCCAAGC AGCCCCGGTC TCCAGGCCAG ATCGTGTCTC CTCTTCTTAA GCAAGTCACT 
GCGATCTTGA ATCCTGTAGT CAAAGTGGCA CCAGCCCTGA TTGAGCCGCT GTATGTGATG 
GCCCAGGTCA AGTATCTCTC AGGGGAGTTA GAGCATGCCC AGAGCACCCT GCAGCGTTGC 
CTGGAGCTGG ACCCCACCTC CGTGGACGCC CACCTCCTCA TGGCTCAGAT CTACCTGGCT 
CAGGACAACT TTGCCATGTG CTCCCACTCC TTGGAGCTGG GCGTCAGCCA CAACTTCCAG 
GTCCGAGACC ACCCGCTTTA CCACTTCATC AAGGCCAAGG CCCTCAACAA GTCTGGGGAC 
TATCCAGAAG CTATAAAGGC ACTGAAGATG ATCATAAAAT TACCAACTCT GAGGACGGAA 
GAGAACAAGA AGTTCCGTGG GCCCTGCGTG CGCCCCAGCG AGCGGGTGTC CATCTTACTG 
GAGCTGGCAG ATGCCCTCCG GATGAATGGA GAGCTGCACG AGGCCACCAA GGTCATGCAG 
GATGCCATCA ACGAGTTCAG CGGCACGCCG GAGGAGATGC GCATCACCAT CGCCAATGTG 
GACTTGGCCC TGAGCAAGGG CAACGTGGAC CTGGCTCTGA GCGTGCTGCA GAGCATCACA 
CCCAAGCAGC CCTGCTACAT GGAAGCCAAG GAGAAGATGG CCAGCATCTA CCTGCAGACC 
CGCAAGGACG CCCGCCTCTA TATCGGGTGC TACCGGGAGC TCTGTGAACA TCTGCCTGGC 
CCCCACACCA GTCTGCTTCT GGGTGATGCT TTCATGAACA TTCAGGAGCC AGAGAAGGCC 
CTGGAGGTCT ATGATGAGGC CTACAGAAAG AACCCACACG ACACATCCCT GATCAGCCGG 
ATCGGGCAGG CTTATGTCAA GACCCACCAG TACACCAAGG CAATTAATTA TTATGAGGCT 
GCCCAGAAGA TTAGCGGACA GGACTTTGTG TGCTGCGATC TGGCCGAACT TCTCCTGAAG 
TTAAAGAAGT TCAACAAAGC AGAAAAAGTT TTGAAGCAGG CACTGGATCA TGACTTTGTC 
AAAGACATCC CATCCATGAT GAATGATGTC AAGTGCTTAC TTTTGCTGGC CAAGGTTTAC 
AAGAGTCATA AAAAAGAAGA TGTGACGGAA ACTTTGAACA AGGCCATGGA CCTCCAGTCT 
CGGATCCTGA AGCGTGTTCC CCTGGAGCGA CCAGAAATGA TCCCCTCCCA GAAGCAACTG 
GCAGCCTCCA TCTGCATCCA GTTTGGGGAA TACTACCTGG CAGAGAAGGA GTATGCCAAG 
GCAGTGCAGT CTTATAAAGA TGCCCTCTCC TACACGCCTA TTAACAATAA GGTGGTGCTG 
GAGCTGGCGA GGCTCTACCT GCTGCAGGGA CACCTGGACC TGTGTGAGCA GCACTGTGCC 
GACCTCCTGC AGACTGAGAA GAACCACGAG GCAGCCTCCG TGGTGATGGC TGACCTGATG 
TTTAGAAGAC AGAAATACGA GGCTGCCATC AACCTCTACC ACCAAGTCCT GGAGAAAGCA 
CCAGACAATT TTCCTGTATT GAACAAACTA ATCGATCTGC TACGAAGAAG TGGCAAACTT 
GAGGCCGCCC CTGCCGTCTT TGAATTGGCC GAGAAGATGT CCAGCCGGGT GCCTTTGGAG 
CCAGGGTTCA ACTTCTGCAG AGGCATCTAC TGCTGGCACA TAGGACAGCC CAATGAAGCC 
CTGAAGTTCC TGAACAAAGC GCGCAAGGAC AGCGCTTGGG GCCAGAGCGC CACCTACCAC 
ATGGTGCAGA TCTGTCTGAA CCCAGACAAC GAGATCGTGG GCGGAGAGGC TTTCGAGAAC 
CTGGTGACCG AGAGCAACCC CAGTGCCCGC AGCTTCAGCA GCAGGAAGGA GTTGGAGCAA 
CATGGCGTGC GCACCGCCGA GAAGCTGCTG CAGGAGTTCT ACCCACACTC GGCCGGGGGC 
CATACCCAGC TGCGGCTGCT GCAGAGCCTG TGCCTGCTGG CGACCAGGGA GAAGGCCAAC 
GTGGAAGTGG CGCTGAGCAC CTTCGTTGAG ATGGCCCAGG CTGAGAAGGA CAGCGTCCCC 
GCCCTGCTGG CCATGGCGCA GGCCTGCTCC CTGCTGAAGC AGGTCCCCAA GGCACGCACG 
CAGCTGAAGC GCCTGGCCAA GGCCCCGTGG GCACTGGCTG AGGCCGATGA CCTGGAGAGG 
AGCTGGCTCC TGCTGGCCGA TATCTACTGC CAGGGCGGCA AGTTTGACCT GGCCTTGGAG 
CTGCTGCAGC GCTGCGTGAA ATACAACAAG TCCTGCTGTA AGGCCTACGA GTACATGGGC 
TTCATCATGG AAAGGGAGCG GTCATACAAG GACGCGGCCT CCAACTACGA GCTGGCCTGG 
CAGTACAGCC GTCACACCAG CCCCGCCATC GGCTTCAGGC TCGCTTTCAA CTACTTGAAA 
GACAAGAGAT TCGTGGAAGC CATTGAAGTC TGCCATGATG TCCTCAGGGA GCACCCCAAC 
TACCCTAAAA TCAGAGAAGA AATTCTGGCA AAGGCCCAAG GGTCTCTGAG GCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128103.1
CDS	131..4087
Translation	MSSNDSSLMAGIIYYSKEKYFRHVQEAAAVGLEKFNNDPVLQFFKAYGVLREEHIQDAISSLESIRNHPDVSLCSIMALIYAHKCCETIDREAIQELESSLKEVRKTASGTALYYAGLFLWLMGLHDKAKEYIDRTLKISSSSREGYVLRGWVDLSSDKPHTVKKSIKYLEHGIQDTKDVLGLMGKVMYFMMLQNYSGALEVVNQITVTCGSFLPALVLKMRLFLARQDWEQTVEMGHRILEKDKSNIDACQILAVHELAREGNMTVAAEHVRNLIKALETREPQNPSLHLKKILVVSRLCGRHQGILQLVCSFIERAFMATSSDAHMATELGYLFILQDQVKEASLWYSKAMKLDESSVAALTGIIWCQILDGHLEEAEHQLEFLKEVQQSLGKSEVLVFLQALLASKKHKGEQEATTLLKEAAELHFSSMQALPMSSEYLERLDPIFLVCIAKEYLVFCPKQPRSPGQIVSPLLKQVTAILNPVVKVAPALIEPLYVMAQVKYLSGELEHAQSTLQRCLELDPTSVDAHLLMAQIYLAQDNFAMCSHSLELGVSHNFQVRDHPLYHFIKAKALNKSGDYPEAIKALKMIIKLPTLRTEENKKFRGPCVRPSERVSILLELADALRMNGELHEATKVMQDAINEFSGTPEEMRITIANVDLALSKGNVDLALSVLQSITPKQPCYMEAKEKMASIYLQTRKDARLYIGCYRELCEHLPGPHTSLLLGDAFMNIQEPEKALEVYDEAYRKNPHDTSLISRIGQAYVKTHQYTKAINYYEAAQKISGQDFVCCDLAELLLKLKKFNKAEKVLKQALDHDFVKDIPSMMNDVKCLLLLAKVYKSHKKEDVTETLNKAMDLQSRILKRVPLERPEMIPSQKQLAASICIQFGEYYLAEKEYAKAVQSYKDALSYTPINNKVVLELARLYLLQGHLDLCEQHCADLLQTEKNHEAASVVMADLMFRRQKYEAAINLYHQVLEKAPDNFPVLNKLIDLLRRSGKLEAAPAVFELAEKMSSRVPLEPGFNFCRGIYCWHIGQPNEALKFLNKARKDSAWGQSATYHMVQICLNPDNEIVGGEAFENLVTESNPSARSFSSRKELEQHGVRTAEKLLQEFYPHSAGGHTQLRLLQSLCLLATREKANVEVALSTFVEMAQAEKDSVPALLAMAQACSLLKQVPKARTQLKRLAKAPWALAEADDLERSWLLLADIYCQGGKFDLALELLQRCVKYNKSCCKAYEYMGFIMERERSYKDAASNYELAWQYSRHTSPAIGFRLAFNYLKDKRFVEAIEVCHDVLREHPNYPKIREEILAKAQGSLRP

Target ORF information:

RefSeq Version	XM_025272318.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025272318.1

ORF Insert Sequence:

ATGAGCAGCA ATGACTCCTC CCTTATGGCT GGGATCATTT ACTACAGCAA AGAAAAGTAC 
TTCCGCCACG TGCAGGAGGC TGCAGCTGTG GGCCTGGAAA AATTCAACAA TGACCCAGTG 
CTGCAGTTCT TTAAAGCCTA TGGAGTCCTC AGGGAAGAGC ACATCCAGGA TGCCATCAGC 
AGCCTGGAGA GCATCCGGAA TCACCCAGAT GTGTCCCTGT GCTCCATCAT GGCCCTCATT 
TATGCTCACA AGTGCTGTGA AACCATTGAC CGAGAAGCAA TTCAGGAGCT TGAGAGCAGC 
CTGAAAGAAG TCCGCAAGAC AGCCAGTGGG ACTGCCCTGT ACTATGCTGG CCTCTTCCTC 
TGGCTCATGG GCCTCCACGA CAAGGCCAAG GAATACATTG ACCGCACGTT GAAGATCTCC 
AGCAGCTCCA GAGAGGGCTA TGTGCTCAGA GGCTGGGTGG ACCTCAGCTC AGACAAACCC 
CACACGGTGA AGAAGTCCAT CAAGTACCTG GAGCATGGCA TTCAGGACAC CAAAGACGTC 
CTGGGACTAA TGGGAAAGGT GATGTACTTC ATGATGCTGC AGAACTACTC GGGGGCCCTT 
GAGGTGGTGA ACCAGATCAC TGTCACCTGC GGGAGCTTCC TGCCTGCCTT GGTCCTAAAG 
ATGCGGCTGT TCCTGGCTCG GCAGGACTGG GAGCAGACGG TAGAAATGGG ACACAGAATC 
CTCGAAAAAG ACAAAAGCAA TATCGATGCC TGCCAAATTC TAGCTGTGCA TGAGCTCGCA 
AGAGAAGGAA ACATGACCGT GGCTGCCGAG CACGTTAGGA ATCTGATTAA GGCACTGGAA 
ACCAGAGAGC CCCAAAATCC AAGCCTCCAT CTTAAAAAAA TTCTTGTGGT GAGCAGACTG 
TGTGGGAGGC ACCAGGGGAT TCTGCAGCTG GTGTGTAGCT TCATCGAACG AGCCTTCATG 
GCCACATCCT CCGACGCCCA CATGGCCACC GAGCTGGGCT ACCTCTTCAT CCTGCAGGAC 
CAAGTAAAGG AGGCGTCTCT GTGGTACTCG AAGGCCATGA AGCTGGACGA GAGCAGCGTG 
GCTGCTTTGA CAGGGATCAT CTGGTGCCAG ATCTTAGACG GCCACCTGGA GGAGGCCGAA 
CACCAGCTGG AATTCCTGAA GGAGGTGCAG CAGTCCCTGG GGAAGTCCGA GGTGCTGGTT 
TTCCTGCAAG CCCTCCTGGC GTCCAAGAAG CATAAGGGGG AGCAGGAGGC CACGACGCTG 
CTAAAGGAGG CCGCTGAGCT GCACTTCTCC AGCATGCAGG CCCTCCCCAT GAGCTCCGAA 
TACTTGGAAC GGCTCGACCC CATCTTCCTG GTCTGCATCG CCAAGGAATA CCTGGTCTTC 
TGCCCCAAGC AGCCCCGGTC TCCAGGCCAG ATCGTGTCTC CTCTTCTTAA GCAAGTCACT 
GCGATCTTGA ATCCTGTAGT CAAAGTGGCA CCAGCCCTGA TTGAGCCGCT GTATGTGATG 
GCCCAGGTCA AGTATCTCTC AGGGGAGTTA GAGCATGCCC AGAGCACCCT GCAGCGTTGC 
CTGGAGCTGG ACCCCACCTC CGTGGACGCC CACCTCCTCA TGGCTCAGAT CTACCTGGCT 
CAGGACAACT TTGCCATGTG CTCCCACTCC TTGGAGCTGG GCGTCAGCCA CAACTTCCAG 
GTCCGAGACC ACCCGCTTTA CCACTTCATC AAGGCCAAGG CCCTCAACAA GTCTGGGGAC 
TATCCAGAAG CTATAAAGGC ACTGAAGATG ATCATAAAAT TACCAACTCT GAGGACGGAA 
GAGAACAAGA AGTTCCGTGG GCCCTGCGTG CGCCCCAGCG AGCGGGTGTC CATCTTACTG 
GAGCTGGCAG ATGCCCTCCG GATGAATGGA GAGCTGCACG AGGCCACCAA GGTCATGCAG 
GATGCCATCA ACGAGTTCAG CGGCACGCCG GAGGAGATGC GCATCACCAT CGCCAATGTG 
GACTTGGCCC TGAGCAAGGG CAACGTGGAC CTGGCTCTGA GCGTGCTGCA GAGCATCACA 
CCCAAGCAGC CCTGCTACAT GGAAGCCAAG GAGAAGATGG CCAGCATCTA CCTGCAGACC 
CGCAAGGACG CCCGCCTCTA TATCGGGTGC TACCGGGAGC TCTGTGAACA TCTGCCTGGC 
CCCCACACCA GTCTGCTTCT GGGTGATGCT TTCATGAACA TTCAGGAGCC AGAGAAGGCC 
CTGGAGGTCT ATGATGAGGC CTACAGAAAG AACCCACACG ACACATCCCT GATCAGCCGG 
ATCGGGCAGG CTTATGTCAA GACCCACCAG TACACCAAGG CAATTAATTA TTATGAGGCT 
GCCCAGAAGA TTAGCGGACA GGACTTTGTG TGCTGCGATC TGGCCGAACT TCTCCTGAAG 
TTAAAGAAGT TCAACAAAGC AGAAAAAGTT TTGAAGCAGG CACTGGATCA TGACTTTGTC 
AAAGACATCC CATCCATGAT GAATGATGTC AAGTGCTTAC TTTTGCTGGC CAAGGTTTAC 
AAGAGTCATA AAAAAGAAGA TGTGACGGAA ACTTTGAACA AGGCCATGGA CCTCCAGTCT 
CGGATCCTGA AGCGTGTTCC CCTGGAGCGA CCAGAAATGA TCCCCTCCCA GAAGCAACTG 
GCAGCCTCCA TCTGCATCCA GTTTGGGGAA TACTACCTGG CAGAGAAGGA GTATGCCAAG 
GCAGTGCAGT CTTATAAAGA TGCCCTCTCC TACACGCCTA TTAACAATAA GGTGGTGCTG 
GAGCTGGCGA GGCTCTACCT GCTGCAGGGA CACCTGGACC TGTGTGAGCA GCACTGTGCC 
GACCTCCTGC AGACTGAGAA GAACCACGAG GCAGCCTCCG TGGTGATGGC TGACCTGATG 
TTTAGAAGAC AGAAATACGA GGCTGCCATC AACCTCTACC ACCAAGTCCT GGAGAAAGCA 
CCAGACAATT TTCCTGTATT GAACAAACTA ATCGATCTGC TACGAAGAAG TGGCAAACTT 
GAGGCCGCCC CTGCCGTCTT TGAATTGGCC GAGAAGATGT CCAGCCGGGT GCCTTTGGAG 
CCAGGGTTCA ACTTCTGCAG AGGCATCTAC TGCTGGCACA TAGGACAGCC CAATGAAGCC 
CTGAAGTTCC TGAACAAAGC GCGCAAGGAC AGCGCTTGGG GCCAGAGCGC CACCTACCAC 
ATGGTGCAGA TCTGTCTGAA CCCAGACAAC GAGATCGTGG GCGGAGAGGC TTTCGAGAAC 
CTGGTGACCG AGAGCAACCC CAGTGCCCGC AGCTTCAGCA GCAGGAAGGA GTTGGAGCAA 
CATGGCGTGC GCACCGCCGA GAAGCTGCTG CAGGAGTTCT ACCCACACTC GGCCGGGGGC 
CATACCCAGC TGCGGCTGCT GCAGAGCCTG TGCCTGCTGG CGACCAGGGA GAAGGCCAAC 
GTGGAAGTGG CGCTGAGCAC CTTCGTTGAG ATGGCCCAGG CTGAGAAGGA CAGCGTCCCC 
GCCCTGCTGG CCATGGCGCA GGCCTGCTCC CTGCTGAAGC AGGTCCCCAA GGCACGCACG 
CAGCTGAAGC GCCTGGCCAA GGCCCCGTGG GCACTGGCTG AGGCCGATGA CCTGGAGAGG 
AGCTGGCTCC TGCTGGCCGA TATCTACTGC CAGGGCGGCA AGTTTGACCT GGCCTTGGAG 
CTGCTGCAGC GCTGCGTGAA ATACAACAAG TCCTGCTGTA AGGCCTACGA GTACATGGGC 
TTCATCATGG AAAGGGAGCG GTCATACAAG GACGCGGCCT CCAACTACGA GCTGGCCTGG 
CAGTACAGCC GTCACACCAG CCCCGCCATC GGCTTCAGGC TCGCTTTCAA CTACTTGAAA 
GACAAGAGAT TCGTGGAAGC CATTGAAGTC TGCCATGATG TCCTCAGGGA GCACCCCAAC 
TACCCTAAAA TCAGAGAAGA AATTCTGGCA AAGGCCCAAG GGTCTCTGAG GCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC21A ( XM_025272319.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128104.1 Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu57655
Clone ID Related Accession (Same CDS sequence)	XM_025272319.1
Accession Version	XM_025272319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	tetratricopeptide repeat protein 21A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA ATGACTCCTC CCTTATGGCT GGGATCATTT ACTACAGCAA AGAAAAGTAC 
TTCCGCCACG TGCAGGAGGC TGCAGCTGTG GGCCTGGAAA AATTCAACAA TGACCCAGTG 
CTGCAGTTCT TTAAAGCCTA TGGAGTCCTC AGGGAAGAGC ACATCCAGGA TGCCATCAGC 
AGCCTGGAGA GCATCCGGAA TCACCCAGAT GTGTCCCTGT GCTCCATCAT GGCCCTCATT 
TATGCTCACA AGTGCTGTGA AACCATTGAC CGAGAAGCAA TTCAGGAGCT TGAGAGCAGC 
CTGAAAGAAG TCCGCAAGAC AGCCAGTGGG ACTGCCCTGT ACTATGCTGG CCTCTTCCTC 
TGGCTCATGG GCCTCCACGA CAAGGCCAAG GAATACATTG ACCGCACGTT GAAGATCTCC 
AGCAGCTCCA GAGAGGGCTA TGTGCTCAGA GGCTGGGTGG ACCTCAGCTC AGACAAACCC 
CACACGGTGA AGAAGTCCAT CAAGTACCTG GAGCATGGCA TTCAGGACAC CAAAGACGTC 
CTGGGACTAA TGGGAAAGGT GATGTACTTC ATGATGCTGC AGAACTACTC GGGGGCCCTT 
GAGGTGGTGA ACCAGATCAC TGTCACCTGC GGGAGCTTCC TGCCTGCCTT GGTCCTAAAG 
ATGCGGCTGT TCCTGGCTCG GCAGGACTGG GAGCAGACGG TAGAAATGGG ACACAGAATC 
CTCGAAAAAG ACAAAAGCAA TATCGATGCC TGCCAAATTC TAGCTGTGCA TGAGCTCGCA 
AGAGAAGGAA ACATGACCGT GGCTGCCGAG CACGTTAGGA ATCTGATTAA GGCACTGGAA 
ACCAGAGAGC CCCAAAATCC AAGCCTCCAT CTTAAAAAAA TTCTTGTGGT GAGCAGACTG 
TGTGGGAGGC ACCAGGGGAT TCTGCAGCTG GTGTGTAGCT TCATCGAACG AGCCTTCATG 
GCCACATCCT CCGACGCCCA CATGGCCACC GAGCTGGGCT ACCTCTTCAT CCTGCAGGAC 
CAAGTAAAGG AGGCGTCTCT GTGGTACTCG AAGGCCATGA AGCTGGACGA GAGCAGCGTG 
GCTGCTTTGA CAGGGATCAT CTGGTGCCAG ATCTTAGACG GCCACCTGGA GGAGGCCGAA 
CACCAGCTGG AATTCCTGAA GGAGGTGCAG CAGTCCCTGG GGAAGTCCGA GGTGCTGGTT 
TTCCTGCAAG CCCTCCTGGC GTCCAAGAAG CATAAGGGGG AGCAGGAGGC CACGACGCTG 
CTAAAGGAGG CCGCTGAGCT GCACTTCTCC AGCATGCAGG CCCTCCCCAT GAGCTCCGAA 
TACTTGGAAC GGCTCGACCC CATCTTCCTG GTCTGCATCG CCAAGGAATA CCTGGTCTTC 
TGCCCCAAGC AGCCCCGGTC TCCAGGCCAG ATCGTGTCTC CTCTTCTTAA GCAAGTCACT 
GCGATCTTGA ATCCTGTAGT CAAAGTGGCA CCAGCCCTGA TTGAGCCGCT GTATGTGATG 
GCCCAGGTCA AGTATCTCTC AGGGGAGTTA GAGCATGCCC AGAGCACCCT GCAGCGTTGC 
CTGGAGCTGG ACCCCACCTC CGTGGACGCC CACCTCCTCA TGGCTCAGAT CTACCTGGCT 
CAGGACAACT TTGCCATGTG CTCCCACTCC TTGGAGCTGG GCGTCAGCCA CAACTTCCAG 
GTCCGAGACC ACCCGCTTTA CCACTTCATC AAGGCCAAGG CCCTCAACAA GTCTGGGGAC 
TATCCAGAAG CTATAAAGGC ACTGAAGATG ATCATAAAAT TACCAACTCT GAGGACGGAA 
GAGAACAAGA AGTTCCGTGG GCCCTGCGTG CGCCCCAGCG AGCGGGTGTC CATCTTACTG 
GAGCTGGCAG ATGCCCTCCG GATGAATGGA GAGCTGCACG AGGCCACCAA GGTCATGCAG 
GATGCCATCA ACGAGTTCAG CGGCACGCCG GAGGAGATGC GCATCACCAT CGCCAATGTG 
GACTTGGCCC TGAGCAAGGG CAACGTGGAC CTGGCTCTGA GCGTGCTGCA GAGCATCACA 
CCCAAGCAGC CCTGCTACAT GGAAGCCAAG GAGAAGATGG CCAGCATCTA CCTGCAGACC 
CGCAAGGACG CCCGCCTCTA TATCGGGTGC TACCGGGAGC TCTGTGAACA TCTGCCTGGC 
CCCCACACCA GTCTGCTTCT GGGTGATGCT TTCATGAACA TTCAGGAGCC AGAGAAGGCC 
CTGGAGGTCT ATGATGAGGC CTACAGAAAG AACCCACACG ACACATCCCT GATCAGCCGG 
ATCGGGCAGG CTTATGTCAA GACCCACCAG TACACCAAGG CAATTAATTA TTATGAGGCT 
GCCCAGAAGA TTAGCGGACA GGACTTTGTG TGCTGCGATC TGGCCGAACT TCTCCTGAAG 
TTAAAGAAGT TCAACAAAGC AGAAAAAGTT TTGAAGCAGG CACTGGATCA TGACTTTGTC 
AAAGACATCC CATCCATGAT GAATGATGTC AAGTGCTTAC TTTTGCTGGC CAAGGTTTAC 
AAGAGTCATA AAAAAGAAGA TGTGACGGAA ACTTTGAACA AGGCCATGGA CCTCCAGTCT 
CGGATCCTGA AGCGTGTTCC CCTGGAGCGA CCAGAAATGA TCCCCTCCCA GAAGCAACTG 
GCAGCCTCCA TCTGCATCCA GTTTGGGGAA TACTACCTGG CAGAGAAGGA GTATGCCAAG 
GCAGTGCAGT CTTATAAAGA TGCCCTCTCC TACACGCCTA TTAACAATAA GGTGGTGCTG 
GAGCTGGCGA GGCTCTACCT GCTGCAGGGA CACCTGGACC TGTGTGAGCA GCACTGTGCC 
GACCTCCTGC AGACTGAGAA GAACCACGAG GCAGCCTCCG TGGTGATGGC TGACCTGATG 
TTTAGAAGAC AGAAATACGA GGCTGCCATC AACCTCTACC ACCAAGTCCT GGAGAAAGCA 
CCAGACAATT TTCCTGTATT GAACAAACTA ATCGATCTGC TACGAAGAAG TGGCAAACTT 
GAGGCCGCCC CTGCCGTCTT TGAATTGGCC GAGAAGATGT CCAGCCGGGT GCCTTTGGAG 
CCAGGGTTCA ACTTCTGCAG AGGCATCTAC TGCTGGCACA TAGGACAGCC CAATGAAGCC 
CTGAAGTTCC TGAACAAAGC GCGCAAGGAC AGCGCTTGGG GCCAGAGCGC CACCTACCAC 
ATGGTGCAGA TCTGTCTGAA CCCAGACAAC GAGATCGTGG GCGGAGAGGC TTTCGAGAAC 
CTGGTGACCG AGAGCAACCC CAGTGCCCGC AGCTTCAGCA GCAGGAAGGA GTTGGAGCAA 
CATGGCGTGC GCACCGCCGA GAAGCTGCTG CAGGAGTTCT ACCCACACTC GGCCGGGGGC 
CATACCCAGC TGCGGCTGCT GCAGAGCCTG TGCCTGCTGG CGACCAGGGA GAAGGCCAAC 
GTGGAAGTGG CGCTGAGCAC CTTCGTTGAG ATGGCCCAGG CTGAGGACAG CGTCCCCGCC 
CTGCTGGCCA TGGCGCAGGC CTGCTCCCTG CTGAAGCAGG TCCCCAAGGC ACGCACGCAG 
CTGAAGCGCC TGGCCAAGGC CCCGTGGGCA CTGGCTGAGG CCGATGACCT GGAGAGGAGC 
TGGCTCCTGC TGGCCGATAT CTACTGCCAG GGCGGCAAGT TTGACCTGGC CTTGGAGCTG 
CTGCAGCGCT GCGTGAAATA CAACAAGTCC TGCTGTAAGG CCTACGAGTA CATGGGCTTC 
ATCATGGAAA GGGAGCGGTC ATACAAGGAC GCGGCCTCCA ACTACGAGCT GGCCTGGCAG 
TACAGCCGTC ACACCAGCCC CGCCATCGGC TTCAGGCTCG CTTTCAACTA CTTGAAAGAC 
AAGAGATTCG TGGAAGCCAT TGAAGTCTGC CATGATGTCC TCAGGGAGCA CCCCAACTAC 
CCTAAAATCA GAGAAGAAAT TCTGGCAAAG GCCCAAGGGT CTCTGAGGCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128104.1
CDS	131..4084
Translation	MSSNDSSLMAGIIYYSKEKYFRHVQEAAAVGLEKFNNDPVLQFFKAYGVLREEHIQDAISSLESIRNHPDVSLCSIMALIYAHKCCETIDREAIQELESSLKEVRKTASGTALYYAGLFLWLMGLHDKAKEYIDRTLKISSSSREGYVLRGWVDLSSDKPHTVKKSIKYLEHGIQDTKDVLGLMGKVMYFMMLQNYSGALEVVNQITVTCGSFLPALVLKMRLFLARQDWEQTVEMGHRILEKDKSNIDACQILAVHELAREGNMTVAAEHVRNLIKALETREPQNPSLHLKKILVVSRLCGRHQGILQLVCSFIERAFMATSSDAHMATELGYLFILQDQVKEASLWYSKAMKLDESSVAALTGIIWCQILDGHLEEAEHQLEFLKEVQQSLGKSEVLVFLQALLASKKHKGEQEATTLLKEAAELHFSSMQALPMSSEYLERLDPIFLVCIAKEYLVFCPKQPRSPGQIVSPLLKQVTAILNPVVKVAPALIEPLYVMAQVKYLSGELEHAQSTLQRCLELDPTSVDAHLLMAQIYLAQDNFAMCSHSLELGVSHNFQVRDHPLYHFIKAKALNKSGDYPEAIKALKMIIKLPTLRTEENKKFRGPCVRPSERVSILLELADALRMNGELHEATKVMQDAINEFSGTPEEMRITIANVDLALSKGNVDLALSVLQSITPKQPCYMEAKEKMASIYLQTRKDARLYIGCYRELCEHLPGPHTSLLLGDAFMNIQEPEKALEVYDEAYRKNPHDTSLISRIGQAYVKTHQYTKAINYYEAAQKISGQDFVCCDLAELLLKLKKFNKAEKVLKQALDHDFVKDIPSMMNDVKCLLLLAKVYKSHKKEDVTETLNKAMDLQSRILKRVPLERPEMIPSQKQLAASICIQFGEYYLAEKEYAKAVQSYKDALSYTPINNKVVLELARLYLLQGHLDLCEQHCADLLQTEKNHEAASVVMADLMFRRQKYEAAINLYHQVLEKAPDNFPVLNKLIDLLRRSGKLEAAPAVFELAEKMSSRVPLEPGFNFCRGIYCWHIGQPNEALKFLNKARKDSAWGQSATYHMVQICLNPDNEIVGGEAFENLVTESNPSARSFSSRKELEQHGVRTAEKLLQEFYPHSAGGHTQLRLLQSLCLLATREKANVEVALSTFVEMAQAEDSVPALLAMAQACSLLKQVPKARTQLKRLAKAPWALAEADDLERSWLLLADIYCQGGKFDLALELLQRCVKYNKSCCKAYEYMGFIMERERSYKDAASNYELAWQYSRHTSPAIGFRLAFNYLKDKRFVEAIEVCHDVLREHPNYPKIREEILAKAQGSLRP

Target ORF information:

RefSeq Version	XM_025272319.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025272319.1

ORF Insert Sequence:

ATGAGCAGCA ATGACTCCTC CCTTATGGCT GGGATCATTT ACTACAGCAA AGAAAAGTAC 
TTCCGCCACG TGCAGGAGGC TGCAGCTGTG GGCCTGGAAA AATTCAACAA TGACCCAGTG 
CTGCAGTTCT TTAAAGCCTA TGGAGTCCTC AGGGAAGAGC ACATCCAGGA TGCCATCAGC 
AGCCTGGAGA GCATCCGGAA TCACCCAGAT GTGTCCCTGT GCTCCATCAT GGCCCTCATT 
TATGCTCACA AGTGCTGTGA AACCATTGAC CGAGAAGCAA TTCAGGAGCT TGAGAGCAGC 
CTGAAAGAAG TCCGCAAGAC AGCCAGTGGG ACTGCCCTGT ACTATGCTGG CCTCTTCCTC 
TGGCTCATGG GCCTCCACGA CAAGGCCAAG GAATACATTG ACCGCACGTT GAAGATCTCC 
AGCAGCTCCA GAGAGGGCTA TGTGCTCAGA GGCTGGGTGG ACCTCAGCTC AGACAAACCC 
CACACGGTGA AGAAGTCCAT CAAGTACCTG GAGCATGGCA TTCAGGACAC CAAAGACGTC 
CTGGGACTAA TGGGAAAGGT GATGTACTTC ATGATGCTGC AGAACTACTC GGGGGCCCTT 
GAGGTGGTGA ACCAGATCAC TGTCACCTGC GGGAGCTTCC TGCCTGCCTT GGTCCTAAAG 
ATGCGGCTGT TCCTGGCTCG GCAGGACTGG GAGCAGACGG TAGAAATGGG ACACAGAATC 
CTCGAAAAAG ACAAAAGCAA TATCGATGCC TGCCAAATTC TAGCTGTGCA TGAGCTCGCA 
AGAGAAGGAA ACATGACCGT GGCTGCCGAG CACGTTAGGA ATCTGATTAA GGCACTGGAA 
ACCAGAGAGC CCCAAAATCC AAGCCTCCAT CTTAAAAAAA TTCTTGTGGT GAGCAGACTG 
TGTGGGAGGC ACCAGGGGAT TCTGCAGCTG GTGTGTAGCT TCATCGAACG AGCCTTCATG 
GCCACATCCT CCGACGCCCA CATGGCCACC GAGCTGGGCT ACCTCTTCAT CCTGCAGGAC 
CAAGTAAAGG AGGCGTCTCT GTGGTACTCG AAGGCCATGA AGCTGGACGA GAGCAGCGTG 
GCTGCTTTGA CAGGGATCAT CTGGTGCCAG ATCTTAGACG GCCACCTGGA GGAGGCCGAA 
CACCAGCTGG AATTCCTGAA GGAGGTGCAG CAGTCCCTGG GGAAGTCCGA GGTGCTGGTT 
TTCCTGCAAG CCCTCCTGGC GTCCAAGAAG CATAAGGGGG AGCAGGAGGC CACGACGCTG 
CTAAAGGAGG CCGCTGAGCT GCACTTCTCC AGCATGCAGG CCCTCCCCAT GAGCTCCGAA 
TACTTGGAAC GGCTCGACCC CATCTTCCTG GTCTGCATCG CCAAGGAATA CCTGGTCTTC 
TGCCCCAAGC AGCCCCGGTC TCCAGGCCAG ATCGTGTCTC CTCTTCTTAA GCAAGTCACT 
GCGATCTTGA ATCCTGTAGT CAAAGTGGCA CCAGCCCTGA TTGAGCCGCT GTATGTGATG 
GCCCAGGTCA AGTATCTCTC AGGGGAGTTA GAGCATGCCC AGAGCACCCT GCAGCGTTGC 
CTGGAGCTGG ACCCCACCTC CGTGGACGCC CACCTCCTCA TGGCTCAGAT CTACCTGGCT 
CAGGACAACT TTGCCATGTG CTCCCACTCC TTGGAGCTGG GCGTCAGCCA CAACTTCCAG 
GTCCGAGACC ACCCGCTTTA CCACTTCATC AAGGCCAAGG CCCTCAACAA GTCTGGGGAC 
TATCCAGAAG CTATAAAGGC ACTGAAGATG ATCATAAAAT TACCAACTCT GAGGACGGAA 
GAGAACAAGA AGTTCCGTGG GCCCTGCGTG CGCCCCAGCG AGCGGGTGTC CATCTTACTG 
GAGCTGGCAG ATGCCCTCCG GATGAATGGA GAGCTGCACG AGGCCACCAA GGTCATGCAG 
GATGCCATCA ACGAGTTCAG CGGCACGCCG GAGGAGATGC GCATCACCAT CGCCAATGTG 
GACTTGGCCC TGAGCAAGGG CAACGTGGAC CTGGCTCTGA GCGTGCTGCA GAGCATCACA 
CCCAAGCAGC CCTGCTACAT GGAAGCCAAG GAGAAGATGG CCAGCATCTA CCTGCAGACC 
CGCAAGGACG CCCGCCTCTA TATCGGGTGC TACCGGGAGC TCTGTGAACA TCTGCCTGGC 
CCCCACACCA GTCTGCTTCT GGGTGATGCT TTCATGAACA TTCAGGAGCC AGAGAAGGCC 
CTGGAGGTCT ATGATGAGGC CTACAGAAAG AACCCACACG ACACATCCCT GATCAGCCGG 
ATCGGGCAGG CTTATGTCAA GACCCACCAG TACACCAAGG CAATTAATTA TTATGAGGCT 
GCCCAGAAGA TTAGCGGACA GGACTTTGTG TGCTGCGATC TGGCCGAACT TCTCCTGAAG 
TTAAAGAAGT TCAACAAAGC AGAAAAAGTT TTGAAGCAGG CACTGGATCA TGACTTTGTC 
AAAGACATCC CATCCATGAT GAATGATGTC AAGTGCTTAC TTTTGCTGGC CAAGGTTTAC 
AAGAGTCATA AAAAAGAAGA TGTGACGGAA ACTTTGAACA AGGCCATGGA CCTCCAGTCT 
CGGATCCTGA AGCGTGTTCC CCTGGAGCGA CCAGAAATGA TCCCCTCCCA GAAGCAACTG 
GCAGCCTCCA TCTGCATCCA GTTTGGGGAA TACTACCTGG CAGAGAAGGA GTATGCCAAG 
GCAGTGCAGT CTTATAAAGA TGCCCTCTCC TACACGCCTA TTAACAATAA GGTGGTGCTG 
GAGCTGGCGA GGCTCTACCT GCTGCAGGGA CACCTGGACC TGTGTGAGCA GCACTGTGCC 
GACCTCCTGC AGACTGAGAA GAACCACGAG GCAGCCTCCG TGGTGATGGC TGACCTGATG 
TTTAGAAGAC AGAAATACGA GGCTGCCATC AACCTCTACC ACCAAGTCCT GGAGAAAGCA 
CCAGACAATT TTCCTGTATT GAACAAACTA ATCGATCTGC TACGAAGAAG TGGCAAACTT 
GAGGCCGCCC CTGCCGTCTT TGAATTGGCC GAGAAGATGT CCAGCCGGGT GCCTTTGGAG 
CCAGGGTTCA ACTTCTGCAG AGGCATCTAC TGCTGGCACA TAGGACAGCC CAATGAAGCC 
CTGAAGTTCC TGAACAAAGC GCGCAAGGAC AGCGCTTGGG GCCAGAGCGC CACCTACCAC 
ATGGTGCAGA TCTGTCTGAA CCCAGACAAC GAGATCGTGG GCGGAGAGGC TTTCGAGAAC 
CTGGTGACCG AGAGCAACCC CAGTGCCCGC AGCTTCAGCA GCAGGAAGGA GTTGGAGCAA 
CATGGCGTGC GCACCGCCGA GAAGCTGCTG CAGGAGTTCT ACCCACACTC GGCCGGGGGC 
CATACCCAGC TGCGGCTGCT GCAGAGCCTG TGCCTGCTGG CGACCAGGGA GAAGGCCAAC 
GTGGAAGTGG CGCTGAGCAC CTTCGTTGAG ATGGCCCAGG CTGAGGACAG CGTCCCCGCC 
CTGCTGGCCA TGGCGCAGGC CTGCTCCCTG CTGAAGCAGG TCCCCAAGGC ACGCACGCAG 
CTGAAGCGCC TGGCCAAGGC CCCGTGGGCA CTGGCTGAGG CCGATGACCT GGAGAGGAGC 
TGGCTCCTGC TGGCCGATAT CTACTGCCAG GGCGGCAAGT TTGACCTGGC CTTGGAGCTG 
CTGCAGCGCT GCGTGAAATA CAACAAGTCC TGCTGTAAGG CCTACGAGTA CATGGGCTTC 
ATCATGGAAA GGGAGCGGTC ATACAAGGAC GCGGCCTCCA ACTACGAGCT GGCCTGGCAG 
TACAGCCGTC ACACCAGCCC CGCCATCGGC TTCAGGCTCG CTTTCAACTA CTTGAAAGAC 
AAGAGATTCG TGGAAGCCAT TGAAGTCTGC CATGATGTCC TCAGGGAGCA CCCCAACTAC 
CCTAAAATCA GAGAAGAAAT TCTGGCAAAG GCCCAAGGGT CTCTGAGGCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC21A ( XM_025272320.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128105.1 Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu57656
Clone ID Related Accession (Same CDS sequence)	XM_025272320.1
Accession Version	XM_025272320.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	tetratricopeptide repeat protein 21A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA ATGACTCCTC CCTTATGGCT GGGATCATTT ACTACAGCAA AGAAAAGTAC 
TTCCGCCACG TGCAGGAGGC TGCAGCTGTG GGCCTGGAAA AATTCAACAA TGACCCAGTG 
CTGCAGTTCT TTAAAGCCTA TGGAGTCCTC AGGGAAGAGC ACATCCAGGA TGCCATCAGC 
AGCCTGGAGA GCATCCGGAA TCACCCAGAT GTGTCCCTGT GCTCCATCAT GGCCCTCATT 
TATGCTCACA AGTGCTGTGA AACCATTGAC CGAGAAGCAA TTCAGGAGCT TGAGAGCAGC 
CTGAAAGAAG TCCGCAAGAC AGCCAGTGGG ACTGCCCTGT ACTATGCTGG CCTCTTCCTC 
TGGCTCATGG GCCTCCACGA CAAGGCCAAG GAATACATTG ACCGCACGTT GAAGATCTCC 
AGCAGCTCCA GAGAGGGCTA TGTGCTCAGA GGCTGGGTGG ACCTCAGCTC AGACAAACCC 
CACACGGTGA AGAAGTCCAT CAAGTACCTG GAGCATGGCA TTCAGGACAC CAAAGACGTC 
CTGGGACTAA TGGGAAAGGT GATGTACTTC ATGATGCTGC AGAACTACTC GGGGGCCCTT 
GAGGTGGTGA ACCAGATCAC TGTCACCTGC GGGAGCTTCC TGCCTGCCTT GGTCCTAAAG 
ATGCGGCTGT TCCTGGCTCG GCAGGACTGG GAGCAGACGG TAGAAATGGG ACACAGAATC 
CTCGAAAAAG ACAAAAGCAA TATCGATGCC TGCCAAATTC TAGCTGTGCA TGAGCTCGCA 
AGAGAAGGAA ACATGACCGT GGCTGCCGAG CACGTTAGGA ATCTGATTAA GGCACTGGAA 
ACCAGAGAGC CCCAAAATCC AAGCCTCCAT CTTAAAAAAA TTCTTGTGGT GAGCAGACTG 
TGTGGGAGGC ACCAGGGGAT TCTGCAGCTG GTGTGTAGCT TCATCGAACG AGCCTTCATG 
GCCACATCCT CCGACGCCCA CATGGCCACC GAGCTGGGCT ACCTCTTCAT CCTGCAGGAC 
CAAGTAAAGG AGGCGTCTCT GTGGTACTCG AAGGCCATGA AGCTGGACGA GAGCAGCGTG 
GCTGCTTTGA CAGGGATCAT CTGGTGCCAG ATCTTAGACG GCCACCTGGA GGAGGCCGAA 
CACCAGCTGG AATTCCTGAA GGAGGTGCAG CAGTCCCTGG GGAAGTCCGA GGTGCTGGTT 
TTCCTGCAAG CCCTCCTGGC GTCCAAGAAG CATAAGGGGG AGCAGGAGGC CACGACGCTG 
CTAAAGGAGG CCGCTGAGCT GCACTTCTCC AGCATGCAGG CCCTCCCCAT GAGCTCCGAA 
TACTTGGAAC GGCTCGACCC CATCTTCCTG GTCTGCATCG CCAAGGAATA CCTGGTCTTC 
TGCCCCAAGC AGCCCCGGTC TCCAGGCCAG ATCGTGTCTC CTCTTCTTAA GCAAGTCACT 
GCGATCTTGA ATCCTGTAGT CAAAGTGGCA CCAGCCCTGA TTGAGCCGCT GTATGTGATG 
GCCCAGGTCA AGTATCTCTC AGGGGAGTTA GAGCATGCCC AGAGCACCCT GCAGCGTTGC 
CTGGAGCTGG ACCCCACCTC CGTGGACGCC CACCTCCTCA TGGCTCAGAT CTACCTGGCT 
CAGGACAACT TTGCCATGTG CTCCCACTCC TTGGAGCTGG GCGTCAGCCA CAACTTCCAG 
GTCCGAGACC ACCCGCTTTA CCACTTCATC AAGGCCAAGG CCCTCAACAA GTCTGGGGAC 
TATCCAGAAG CTATAAAGGC ACTGAAGATG ATCATAAAAT TACCAACTCT GAGGACGGAA 
GAGAACAAGA AGTTCCGTGG GCCCTGCGTG CGCCCCAGCG AGCGGGTGTC CATCTTACTG 
GAGCTGGCAG ATGCCCTCCG GATGAATGGA GAGCTGCACG AGGCCACCAA GGTCATGCAG 
GATGCCATCA ACGAGTTCAG CGGCACGCCG GAGGAGATGC GCATCACCAT CGCCAATGTG 
GACTTGGCCC TGAGCAAGGG CAACGTGGAC CTGGCTCTGA GCGTGCTGCA GAGCATCACA 
CCCAAGCAGC CCTGCTACAT GGAAGCCAAG GAGAAGATGG CCAGCATCTA CCTGCAGACC 
CGCAAGGACG CCCGCCTCTA TATCGGGTGC TACCGGGAGC TCTGTGAACA TCTGCCTGGC 
CCCCACACCA GTCTGCTTCT GGGTGATGCT TTCATGAACA TTCAGGAGCC AGAGAAGGCC 
CTGGAGGTCT ATGATGAGGC CTACAGAAAG AACCCACACG ACACATCCCT GATCAGCCGG 
ATCGGGCAGG CTTATGTCAA GACCCACCAG TACACCAAGG CAATTAATTA TTATGAGGCT 
GCCCAGAAGA TTAGCGGACA GGACTTTGTG TGCTGCGATC TGGCCGAACT TCTCCTGAAG 
TTAAAGAAGT TCAACAAAGC AGAAAAAGTT TTGAAGCAGG CACTGGATCA TGACTTTGTC 
AAAGACATCC CATCCATGAT GAATGATGTC AAGTGCTTAC TTTTGCTGGC CAAGGTTTAC 
AAGAGTCATA AAAAAGAAGA TGTGACGGAA ACTTTGAACA AGGCCATGGA CCTCCAGTCT 
CGGATCCTGA AGCGTGTTCC CCTGGAGCGA CCAGAAATGA TCCCCTCCCA GAAGCAACTG 
GCAGCCTCCA TCTGCATCCA GTTTGGGGAA TACTACCTGG CAGAGAAGGA GTATGCCAAG 
GCAGTGCAGT CTTATAAAGA TGCCCTCTCC TACACGCCTA TTAACAATAA GGTGGTGCTG 
GAGCTGGCGA GGCTCTACCT GCTGCAGGGA CACCTGGACC TGTGTGAGCA GCACTGTGCC 
GACCTCCTGC AGACTGAGAA GAACCACGAG GCAGCCTCCG TGGTGATGGC TGACCTGATG 
TTTAGAAGAC AGAAATACGA GGCTGCCATC AACCTCTACC ACCAAGTCCT GGAGAAAGCA 
CCAGACAATT TTCCTGTATT GAACAAACTA ATCGATCTGC TACGAAGAAG TGGCAAACTT 
GAGGCCGCCC CTGCCGTCTT TGAATTGGCC GAGAAGATGT CCAGCCGGGT GCCTTTGGAG 
CCAGGGTTCA ACTTCTGCAG AGGCATCTAC TGCTGGCACA TAGGACAGCC CAATGAAGCC 
CTGAAGTTCC TGAACAAAGC GCGCAAGGAC AGCGCTTGGG GCCAGAGCGC CACCTACCAC 
ATGGTGCAGA TCTGTCTGAA CCCAGACAAC GAGATCGTGG GCGGAGAGGC TTTCGAGAAC 
CTGGTGACCG AGAGCAACTT CAGCAGCAGG AAGGAGTTGG AGCAACATGG CGTGCGCACC 
GCCGAGAAGC TGCTGCAGGA GTTCTACCCA CACTCGGCCG GGGGCCATAC CCAGCTGCGG 
CTGCTGCAGA GCCTGTGCCT GCTGGCGACC AGGGAGAAGG CCAACGTGGA AGTGGCGCTG 
AGCACCTTCG TTGAGATGGC CCAGGCTGAG AAGGACAGCG TCCCCGCCCT GCTGGCCATG 
GCGCAGGCCT GCTCCCTGCT GAAGCAGGTC CCCAAGGCAC GCACGCAGCT GAAGCGCCTG 
GCCAAGGCCC CGTGGGCACT GGCTGAGGCC GATGACCTGG AGAGGAGCTG GCTCCTGCTG 
GCCGATATCT ACTGCCAGGG CGGCAAGTTT GACCTGGCCT TGGAGCTGCT GCAGCGCTGC 
GTGAAATACA ACAAGTCCTG CTGTAAGGCC TACGAGTACA TGGGCTTCAT CATGGAAAGG 
GAGCGGTCAT ACAAGGACGC GGCCTCCAAC TACGAGCTGG CCTGGCAGTA CAGCCGTCAC 
ACCAGCCCCG CCATCGGCTT CAGGCTCGCT TTCAACTACT TGAAAGACAA GAGATTCGTG 
GAAGCCATTG AAGTCTGCCA TGATGTCCTC AGGGAGCACC CCAACTACCC TAAAATCAGA 
GAAGAAATTC TGGCAAAGGC CCAAGGGTCT CTGAGGCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128105.1
CDS	1..3942
Translation	MSSNDSSLMAGIIYYSKEKYFRHVQEAAAVGLEKFNNDPVLQFFKAYGVLREEHIQDAISSLESIRNHPDVSLCSIMALIYAHKCCETIDREAIQELESSLKEVRKTASGTALYYAGLFLWLMGLHDKAKEYIDRTLKISSSSREGYVLRGWVDLSSDKPHTVKKSIKYLEHGIQDTKDVLGLMGKVMYFMMLQNYSGALEVVNQITVTCGSFLPALVLKMRLFLARQDWEQTVEMGHRILEKDKSNIDACQILAVHELAREGNMTVAAEHVRNLIKALETREPQNPSLHLKKILVVSRLCGRHQGILQLVCSFIERAFMATSSDAHMATELGYLFILQDQVKEASLWYSKAMKLDESSVAALTGIIWCQILDGHLEEAEHQLEFLKEVQQSLGKSEVLVFLQALLASKKHKGEQEATTLLKEAAELHFSSMQALPMSSEYLERLDPIFLVCIAKEYLVFCPKQPRSPGQIVSPLLKQVTAILNPVVKVAPALIEPLYVMAQVKYLSGELEHAQSTLQRCLELDPTSVDAHLLMAQIYLAQDNFAMCSHSLELGVSHNFQVRDHPLYHFIKAKALNKSGDYPEAIKALKMIIKLPTLRTEENKKFRGPCVRPSERVSILLELADALRMNGELHEATKVMQDAINEFSGTPEEMRITIANVDLALSKGNVDLALSVLQSITPKQPCYMEAKEKMASIYLQTRKDARLYIGCYRELCEHLPGPHTSLLLGDAFMNIQEPEKALEVYDEAYRKNPHDTSLISRIGQAYVKTHQYTKAINYYEAAQKISGQDFVCCDLAELLLKLKKFNKAEKVLKQALDHDFVKDIPSMMNDVKCLLLLAKVYKSHKKEDVTETLNKAMDLQSRILKRVPLERPEMIPSQKQLAASICIQFGEYYLAEKEYAKAVQSYKDALSYTPINNKVVLELARLYLLQGHLDLCEQHCADLLQTEKNHEAASVVMADLMFRRQKYEAAINLYHQVLEKAPDNFPVLNKLIDLLRRSGKLEAAPAVFELAEKMSSRVPLEPGFNFCRGIYCWHIGQPNEALKFLNKARKDSAWGQSATYHMVQICLNPDNEIVGGEAFENLVTESNFSSRKELEQHGVRTAEKLLQEFYPHSAGGHTQLRLLQSLCLLATREKANVEVALSTFVEMAQAEKDSVPALLAMAQACSLLKQVPKARTQLKRLAKAPWALAEADDLERSWLLLADIYCQGGKFDLALELLQRCVKYNKSCCKAYEYMGFIMERERSYKDAASNYELAWQYSRHTSPAIGFRLAFNYLKDKRFVEAIEVCHDVLREHPNYPKIREEILAKAQGSLRP

Target ORF information:

RefSeq Version	XM_025272320.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025272320.1

ORF Insert Sequence:

ATGAGCAGCA ATGACTCCTC CCTTATGGCT GGGATCATTT ACTACAGCAA AGAAAAGTAC 
TTCCGCCACG TGCAGGAGGC TGCAGCTGTG GGCCTGGAAA AATTCAACAA TGACCCAGTG 
CTGCAGTTCT TTAAAGCCTA TGGAGTCCTC AGGGAAGAGC ACATCCAGGA TGCCATCAGC 
AGCCTGGAGA GCATCCGGAA TCACCCAGAT GTGTCCCTGT GCTCCATCAT GGCCCTCATT 
TATGCTCACA AGTGCTGTGA AACCATTGAC CGAGAAGCAA TTCAGGAGCT TGAGAGCAGC 
CTGAAAGAAG TCCGCAAGAC AGCCAGTGGG ACTGCCCTGT ACTATGCTGG CCTCTTCCTC 
TGGCTCATGG GCCTCCACGA CAAGGCCAAG GAATACATTG ACCGCACGTT GAAGATCTCC 
AGCAGCTCCA GAGAGGGCTA TGTGCTCAGA GGCTGGGTGG ACCTCAGCTC AGACAAACCC 
CACACGGTGA AGAAGTCCAT CAAGTACCTG GAGCATGGCA TTCAGGACAC CAAAGACGTC 
CTGGGACTAA TGGGAAAGGT GATGTACTTC ATGATGCTGC AGAACTACTC GGGGGCCCTT 
GAGGTGGTGA ACCAGATCAC TGTCACCTGC GGGAGCTTCC TGCCTGCCTT GGTCCTAAAG 
ATGCGGCTGT TCCTGGCTCG GCAGGACTGG GAGCAGACGG TAGAAATGGG ACACAGAATC 
CTCGAAAAAG ACAAAAGCAA TATCGATGCC TGCCAAATTC TAGCTGTGCA TGAGCTCGCA 
AGAGAAGGAA ACATGACCGT GGCTGCCGAG CACGTTAGGA ATCTGATTAA GGCACTGGAA 
ACCAGAGAGC CCCAAAATCC AAGCCTCCAT CTTAAAAAAA TTCTTGTGGT GAGCAGACTG 
TGTGGGAGGC ACCAGGGGAT TCTGCAGCTG GTGTGTAGCT TCATCGAACG AGCCTTCATG 
GCCACATCCT CCGACGCCCA CATGGCCACC GAGCTGGGCT ACCTCTTCAT CCTGCAGGAC 
CAAGTAAAGG AGGCGTCTCT GTGGTACTCG AAGGCCATGA AGCTGGACGA GAGCAGCGTG 
GCTGCTTTGA CAGGGATCAT CTGGTGCCAG ATCTTAGACG GCCACCTGGA GGAGGCCGAA 
CACCAGCTGG AATTCCTGAA GGAGGTGCAG CAGTCCCTGG GGAAGTCCGA GGTGCTGGTT 
TTCCTGCAAG CCCTCCTGGC GTCCAAGAAG CATAAGGGGG AGCAGGAGGC CACGACGCTG 
CTAAAGGAGG CCGCTGAGCT GCACTTCTCC AGCATGCAGG CCCTCCCCAT GAGCTCCGAA 
TACTTGGAAC GGCTCGACCC CATCTTCCTG GTCTGCATCG CCAAGGAATA CCTGGTCTTC 
TGCCCCAAGC AGCCCCGGTC TCCAGGCCAG ATCGTGTCTC CTCTTCTTAA GCAAGTCACT 
GCGATCTTGA ATCCTGTAGT CAAAGTGGCA CCAGCCCTGA TTGAGCCGCT GTATGTGATG 
GCCCAGGTCA AGTATCTCTC AGGGGAGTTA GAGCATGCCC AGAGCACCCT GCAGCGTTGC 
CTGGAGCTGG ACCCCACCTC CGTGGACGCC CACCTCCTCA TGGCTCAGAT CTACCTGGCT 
CAGGACAACT TTGCCATGTG CTCCCACTCC TTGGAGCTGG GCGTCAGCCA CAACTTCCAG 
GTCCGAGACC ACCCGCTTTA CCACTTCATC AAGGCCAAGG CCCTCAACAA GTCTGGGGAC 
TATCCAGAAG CTATAAAGGC ACTGAAGATG ATCATAAAAT TACCAACTCT GAGGACGGAA 
GAGAACAAGA AGTTCCGTGG GCCCTGCGTG CGCCCCAGCG AGCGGGTGTC CATCTTACTG 
GAGCTGGCAG ATGCCCTCCG GATGAATGGA GAGCTGCACG AGGCCACCAA GGTCATGCAG 
GATGCCATCA ACGAGTTCAG CGGCACGCCG GAGGAGATGC GCATCACCAT CGCCAATGTG 
GACTTGGCCC TGAGCAAGGG CAACGTGGAC CTGGCTCTGA GCGTGCTGCA GAGCATCACA 
CCCAAGCAGC CCTGCTACAT GGAAGCCAAG GAGAAGATGG CCAGCATCTA CCTGCAGACC 
CGCAAGGACG CCCGCCTCTA TATCGGGTGC TACCGGGAGC TCTGTGAACA TCTGCCTGGC 
CCCCACACCA GTCTGCTTCT GGGTGATGCT TTCATGAACA TTCAGGAGCC AGAGAAGGCC 
CTGGAGGTCT ATGATGAGGC CTACAGAAAG AACCCACACG ACACATCCCT GATCAGCCGG 
ATCGGGCAGG CTTATGTCAA GACCCACCAG TACACCAAGG CAATTAATTA TTATGAGGCT 
GCCCAGAAGA TTAGCGGACA GGACTTTGTG TGCTGCGATC TGGCCGAACT TCTCCTGAAG 
TTAAAGAAGT TCAACAAAGC AGAAAAAGTT TTGAAGCAGG CACTGGATCA TGACTTTGTC 
AAAGACATCC CATCCATGAT GAATGATGTC AAGTGCTTAC TTTTGCTGGC CAAGGTTTAC 
AAGAGTCATA AAAAAGAAGA TGTGACGGAA ACTTTGAACA AGGCCATGGA CCTCCAGTCT 
CGGATCCTGA AGCGTGTTCC CCTGGAGCGA CCAGAAATGA TCCCCTCCCA GAAGCAACTG 
GCAGCCTCCA TCTGCATCCA GTTTGGGGAA TACTACCTGG CAGAGAAGGA GTATGCCAAG 
GCAGTGCAGT CTTATAAAGA TGCCCTCTCC TACACGCCTA TTAACAATAA GGTGGTGCTG 
GAGCTGGCGA GGCTCTACCT GCTGCAGGGA CACCTGGACC TGTGTGAGCA GCACTGTGCC 
GACCTCCTGC AGACTGAGAA GAACCACGAG GCAGCCTCCG TGGTGATGGC TGACCTGATG 
TTTAGAAGAC AGAAATACGA GGCTGCCATC AACCTCTACC ACCAAGTCCT GGAGAAAGCA 
CCAGACAATT TTCCTGTATT GAACAAACTA ATCGATCTGC TACGAAGAAG TGGCAAACTT 
GAGGCCGCCC CTGCCGTCTT TGAATTGGCC GAGAAGATGT CCAGCCGGGT GCCTTTGGAG 
CCAGGGTTCA ACTTCTGCAG AGGCATCTAC TGCTGGCACA TAGGACAGCC CAATGAAGCC 
CTGAAGTTCC TGAACAAAGC GCGCAAGGAC AGCGCTTGGG GCCAGAGCGC CACCTACCAC 
ATGGTGCAGA TCTGTCTGAA CCCAGACAAC GAGATCGTGG GCGGAGAGGC TTTCGAGAAC 
CTGGTGACCG AGAGCAACTT CAGCAGCAGG AAGGAGTTGG AGCAACATGG CGTGCGCACC 
GCCGAGAAGC TGCTGCAGGA GTTCTACCCA CACTCGGCCG GGGGCCATAC CCAGCTGCGG 
CTGCTGCAGA GCCTGTGCCT GCTGGCGACC AGGGAGAAGG CCAACGTGGA AGTGGCGCTG 
AGCACCTTCG TTGAGATGGC CCAGGCTGAG AAGGACAGCG TCCCCGCCCT GCTGGCCATG 
GCGCAGGCCT GCTCCCTGCT GAAGCAGGTC CCCAAGGCAC GCACGCAGCT GAAGCGCCTG 
GCCAAGGCCC CGTGGGCACT GGCTGAGGCC GATGACCTGG AGAGGAGCTG GCTCCTGCTG 
GCCGATATCT ACTGCCAGGG CGGCAAGTTT GACCTGGCCT TGGAGCTGCT GCAGCGCTGC 
GTGAAATACA ACAAGTCCTG CTGTAAGGCC TACGAGTACA TGGGCTTCAT CATGGAAAGG 
GAGCGGTCAT ACAAGGACGC GGCCTCCAAC TACGAGCTGG CCTGGCAGTA CAGCCGTCAC 
ACCAGCCCCG CCATCGGCTT CAGGCTCGCT TTCAACTACT TGAAAGACAA GAGATTCGTG 
GAAGCCATTG AAGTCTGCCA TGATGTCCTC AGGGAGCACC CCAACTACCC TAAAATCAGA 
GAAGAAATTC TGGCAAAGGC CCAAGGGTCT CTGAGGCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC21A ( XM_025272321.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128106.1 Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X6, mRNA.

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CloneID	OBu57657
Clone ID Related Accession (Same CDS sequence)	XM_025272321.1
Accession Version	XM_025272321.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	tetratricopeptide repeat protein 21A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA ATGACTCCTC CCTTATGGCT GGGATCATTT ACTACAGCAA AGAAAAGTAC 
TTCCGCCACG TGCAGGAGGC TGCAGCTGTG GGCCTGGAAA AATTCAACAA TGACCCAGTG 
CTGCAGTTCT TTAAAGCCTA TGGAGTCCTC AGGGAAGAGC ACATCCAGGA TGCCATCAGC 
AGCCTGGAGA GCATCCGGAA TCACCCAGAT GTGTCCCTGT GCTCCATCAT GGCCCTCATT 
TATGCTCACA AGTGCTGTGA AACCATTGAC CGAGAAGCAA TTCAGGAGCT TGAGAGCAGC 
CTGAAAGAAG TCCGCAAGAC AGCCAGTGGG ACTGCCCTGT ACTATGCTGG CCTCTTCCTC 
TGGCTCATGG GCCTCCACGA CAAGGCCAAG GAATACATTG ACCGCACGTT GAAGATCTCC 
AGCAGCTCCA GAGAGGGCTA TGTGCTCAGA GGCTGGGTGG ACCTCAGCTC AGACAAACCC 
CACACGGTGA AGAAGTCCAT CAAGTACCTG GAGCATGGCA TTCAGGACAC CAAAGACGTC 
CTGGGACTAA TGGGAAAGGT GATGTACTTC ATGATGCTGC AGAACTACTC GGGGGCCCTT 
GAGGTGGTGA ACCAGATCAC TGTCACCTGC GGGAGCTTCC TGCCTGCCTT GGTCCTAAAG 
ATGCGGCTGT TCCTGGCTCG GCAGGACTGG GAGCAGACGG TAGAAATGGG ACACAGAATC 
CTCGAAAAAG ACAAAAGCAA TATCGATGCC TGCCAAATTC TAGCTGTGCA TGAGCTCGCA 
AGAGAAGGAA ACATGACCGT GGCTGCCGAG CACGTTAGGA ATCTGATTAA GGCACTGGAA 
ACCAGAGAGC CCCAAAATCC AAGCCTCCAT CTTAAAAAAA TTCTTGTGGT GAGCAGACTG 
TGTGGGAGGC ACCAGGGGAT TCTGCAGCTG GTGTGTAGCT TCATCGAACG AGCCTTCATG 
GCCACATCCT CCGACGCCCA CATGGCCACC GAGCTGGGCT ACCTCTTCAT CCTGCAGGAC 
CAAGTAAAGG AGGCGTCTCT GTGGTACTCG AAGGCCATGA AGCTGGACGA GAGCAGCGTG 
GCTGCTTTGA CAGGGATCAT CTGGTGCCAG ATCTTAGACG GCCACCTGGA GGAGGCCGAA 
CACCAGCTGG AATTCCTGAA GGAGGTGCAG CAGTCCCTGG GGAAGTCCGA GGTGCTGGTT 
TTCCTGCAAG CCCTCCTGGC GTCCAAGAAG CATAAGGGGG AGCAGGAGGC CACGACGCTG 
CTAAAGGAGG CCGCTGAGCT GCACTTCTCC AGCATGCAGG CCCTCCCCAT GAGCTCCGAA 
TACTTGGAAC GGCTCGACCC CATCTTCCTG GTCTGCATCG CCAAGGAATA CCTGGTCTTC 
TGCCCCAAGC AGCCCCGGTC TCCAGGCCAG ATCGTGTCTC CTCTTCTTAA GCAAGTCACT 
GCGATCTTGA ATCCTGTAGT CAAAGTGGCA CCAGCCCTGA TTGAGCCGCT GTATGTGATG 
GCCCAGGTCA AGTATCTCTC AGGGGAGTTA GAGCATGCCC AGAGCACCCT GCAGCGTTGC 
CTGGAGCTGG ACCCCACCTC CGTGGACGCC CACCTCCTCA TGGCTCAGAT CTACCTGGCT 
CAGGACAACT TTGCCATGTG CTCCCACTCC TTGGAGCTGG GCGTCAGCCA CAACTTCCAG 
GTCCGAGACC ACCCGCTTTA CCACTTCATC AAGGCCAAGG CCCTCAACAA GTCTGGGGAC 
TATCCAGAAG CTATAAAGGC ACTGAAGATG ATCATAAAAT TACCAACTCT GAGGACGGAA 
GAGAACAAGA AGTTCCGTGG GCCCTGCGTG CGCCCCAGCG AGCGGGTGTC CATCTTACTG 
GAGCTGGCAG ATGCCCTCCG GATGAATGGA GAGCTGCACG AGGCCACCAA GGTCATGCAG 
GATGCCATCA ACGAGTTCAG CGGCACGCCG GAGGAGATGC GCATCACCAT CGCCAATGTG 
GACTTGGCCC TGAGCAAGGG CAACGTGGAC CTGGCTCTGA GCGTGCTGCA GAGCATCACA 
CCCAAGCAGC CCTGCTACAT GGAAGCCAAG GAGAAGATGG CCAGCATCTA CCTGCAGACC 
CGCAAGGACG CCCGCCTCTA TATCGGGTGC TACCGGGAGC TCTGTGAACA TCTGCCTGGC 
CCCCACACCA GTCTGCTTCT GGGTGATGCT TTCATGAACA TTCAGGAGCC AGAGAAGGCC 
CTGGAGGTCT ATGATGAGGC CTACAGAAAG AACCCACACG ACACATCCCT GATCAGCCGG 
ATCGGGCAGG CTTATGTCAA GACCCACCAG TACACCAAGG CAATTAATTA TTATGAGGCT 
GCCCAGAAGA TTAGCGGACA GGACTTTGTG TGCTGCGATC TGGCCGAACT TCTCCTGAAG 
TTAAAGAAGT TCAACAAAGC AGAAAAAGTT TTGAAGCAGG CACTGGATCA TGACTTTGTC 
AAAGACATCC CATCCATGAT GAATGATGTC AAGTGCTTAC TTTTGCTGGC CAAGGTTTAC 
AAGAGTCATA AAAAAGAAGA TGTGACGGAA ACTTTGAACA AGGCCATGGA CCTCCAGTCT 
CGGATCCTGA AGCGTGTTCC CCTGGAGCGA CCAGAAATGA TCCCCTCCCA GAAGCAACTG 
GCAGCCTCCA TCTGCATCCA GTTTGGGGAA TACTACCTGG CAGAGAAGGA GTATGCCAAG 
GCAGTGCAGT CTTATAAAGA TGCCCTCTCC TACACGCCTA TTAACAATAA GGTGGTGCTG 
GAGCTGGCGA GGCTCTACCT GCTGCAGGGA CACCTGGACC TGTGTGAGCA GCACTGTGCC 
GACCTCCTGC AGACTGAGAA GAACCACGAG GCAGCCTCCG TGGTGATGGC TGACCTGATG 
TTTAGAAGAC AGAAATACGA GGCTGCCATC AACCTCTACC ACCAAGTCCT GGAGAAAGCA 
CCAGACAATT TTCCTGTATT GAACAAACTA ATCGATCTGC TACGAAGAAG TGGCAAACTT 
GAGGCCGCCC CTGCCGTCTT TGAATTGGCC GAGAAGATGT CCAGCCGGGT GCCTTTGGAG 
CCAGGGTTCA ACTTCTGCAG AGGCATCTAC TGCTGGCACA TAGGACAGCC CAATGAAGCC 
CTGAAGTTCC TGAACAAAGC GCGCAAGGAC AGCGCTTGGG GCCAGAGCGC CACCTACCAC 
ATGGTGCAGA TCTGTCTGAA CCCAGACAAC GAGATCGTGG GCGGAGAGGC TTTCGAGAAC 
CTGGTGACCG AGAGCAACCC CAGTGCCCGC AGCTTCAGCA GCAGGAAGGA GTTGGAGCAA 
CATGGCGTGC GCACCGCCGA GAAGCTGCTG CAGGAGTTCT ACCCACACTC GGCCGGGGGC 
CATACCCAGC TGCGGCTGCT GCAGAGCCTG TGCCTGCTGG CGACCAGGGA GAAGGCCAAC 
GTGGAAGTGG CGCTGAGCAC CTTCGTTGAG ATGGCCCAGG CTGAGAAGGA CAGCGTCCCC 
GCCCTGCTGG CCATGGCGCA GGCCTGCTCC CTGCTGAAGC AGGTCCCCAA GGCACGCACG 
CAGCTGAAGC GCCTGGCCAA GGCCCCGTGG GCACTGGCTG AGGCCGATGA CCTGGAGAGG 
AGCTGGCTCC TGCTGGCCGA TATCTACTGC CAGGGCGGCA AGTTTGACCT GGCCTTGGAG 
CTGCTGCAGC GCTGCGTGAA ATACAACAAG TCCTGCTGTA AGGCCTACGA GTACATGGGC 
TTCATCATGG AAAGGGAGCG GTCATACAAG GACGCGGCCT CCAACTACGA GCTGGCCTGG 
CAGTACAGCC GTCACACCAG CCCCGCCATC GGTACAGTCG TCGGGCAGGC ACGCGCTTCA 
GGCTCGCTTT CAACTACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128106.1
CDS	131..3991
Translation	MSSNDSSLMAGIIYYSKEKYFRHVQEAAAVGLEKFNNDPVLQFFKAYGVLREEHIQDAISSLESIRNHPDVSLCSIMALIYAHKCCETIDREAIQELESSLKEVRKTASGTALYYAGLFLWLMGLHDKAKEYIDRTLKISSSSREGYVLRGWVDLSSDKPHTVKKSIKYLEHGIQDTKDVLGLMGKVMYFMMLQNYSGALEVVNQITVTCGSFLPALVLKMRLFLARQDWEQTVEMGHRILEKDKSNIDACQILAVHELAREGNMTVAAEHVRNLIKALETREPQNPSLHLKKILVVSRLCGRHQGILQLVCSFIERAFMATSSDAHMATELGYLFILQDQVKEASLWYSKAMKLDESSVAALTGIIWCQILDGHLEEAEHQLEFLKEVQQSLGKSEVLVFLQALLASKKHKGEQEATTLLKEAAELHFSSMQALPMSSEYLERLDPIFLVCIAKEYLVFCPKQPRSPGQIVSPLLKQVTAILNPVVKVAPALIEPLYVMAQVKYLSGELEHAQSTLQRCLELDPTSVDAHLLMAQIYLAQDNFAMCSHSLELGVSHNFQVRDHPLYHFIKAKALNKSGDYPEAIKALKMIIKLPTLRTEENKKFRGPCVRPSERVSILLELADALRMNGELHEATKVMQDAINEFSGTPEEMRITIANVDLALSKGNVDLALSVLQSITPKQPCYMEAKEKMASIYLQTRKDARLYIGCYRELCEHLPGPHTSLLLGDAFMNIQEPEKALEVYDEAYRKNPHDTSLISRIGQAYVKTHQYTKAINYYEAAQKISGQDFVCCDLAELLLKLKKFNKAEKVLKQALDHDFVKDIPSMMNDVKCLLLLAKVYKSHKKEDVTETLNKAMDLQSRILKRVPLERPEMIPSQKQLAASICIQFGEYYLAEKEYAKAVQSYKDALSYTPINNKVVLELARLYLLQGHLDLCEQHCADLLQTEKNHEAASVVMADLMFRRQKYEAAINLYHQVLEKAPDNFPVLNKLIDLLRRSGKLEAAPAVFELAEKMSSRVPLEPGFNFCRGIYCWHIGQPNEALKFLNKARKDSAWGQSATYHMVQICLNPDNEIVGGEAFENLVTESNPSARSFSSRKELEQHGVRTAEKLLQEFYPHSAGGHTQLRLLQSLCLLATREKANVEVALSTFVEMAQAEKDSVPALLAMAQACSLLKQVPKARTQLKRLAKAPWALAEADDLERSWLLLADIYCQGGKFDLALELLQRCVKYNKSCCKAYEYMGFIMERERSYKDAASNYELAWQYSRHTSPAIGTVVGQARASGSLSTT

Target ORF information:

RefSeq Version	XM_025272321.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025272321.1

ORF Insert Sequence:

ATGAGCAGCA ATGACTCCTC CCTTATGGCT GGGATCATTT ACTACAGCAA AGAAAAGTAC 
TTCCGCCACG TGCAGGAGGC TGCAGCTGTG GGCCTGGAAA AATTCAACAA TGACCCAGTG 
CTGCAGTTCT TTAAAGCCTA TGGAGTCCTC AGGGAAGAGC ACATCCAGGA TGCCATCAGC 
AGCCTGGAGA GCATCCGGAA TCACCCAGAT GTGTCCCTGT GCTCCATCAT GGCCCTCATT 
TATGCTCACA AGTGCTGTGA AACCATTGAC CGAGAAGCAA TTCAGGAGCT TGAGAGCAGC 
CTGAAAGAAG TCCGCAAGAC AGCCAGTGGG ACTGCCCTGT ACTATGCTGG CCTCTTCCTC 
TGGCTCATGG GCCTCCACGA CAAGGCCAAG GAATACATTG ACCGCACGTT GAAGATCTCC 
AGCAGCTCCA GAGAGGGCTA TGTGCTCAGA GGCTGGGTGG ACCTCAGCTC AGACAAACCC 
CACACGGTGA AGAAGTCCAT CAAGTACCTG GAGCATGGCA TTCAGGACAC CAAAGACGTC 
CTGGGACTAA TGGGAAAGGT GATGTACTTC ATGATGCTGC AGAACTACTC GGGGGCCCTT 
GAGGTGGTGA ACCAGATCAC TGTCACCTGC GGGAGCTTCC TGCCTGCCTT GGTCCTAAAG 
ATGCGGCTGT TCCTGGCTCG GCAGGACTGG GAGCAGACGG TAGAAATGGG ACACAGAATC 
CTCGAAAAAG ACAAAAGCAA TATCGATGCC TGCCAAATTC TAGCTGTGCA TGAGCTCGCA 
AGAGAAGGAA ACATGACCGT GGCTGCCGAG CACGTTAGGA ATCTGATTAA GGCACTGGAA 
ACCAGAGAGC CCCAAAATCC AAGCCTCCAT CTTAAAAAAA TTCTTGTGGT GAGCAGACTG 
TGTGGGAGGC ACCAGGGGAT TCTGCAGCTG GTGTGTAGCT TCATCGAACG AGCCTTCATG 
GCCACATCCT CCGACGCCCA CATGGCCACC GAGCTGGGCT ACCTCTTCAT CCTGCAGGAC 
CAAGTAAAGG AGGCGTCTCT GTGGTACTCG AAGGCCATGA AGCTGGACGA GAGCAGCGTG 
GCTGCTTTGA CAGGGATCAT CTGGTGCCAG ATCTTAGACG GCCACCTGGA GGAGGCCGAA 
CACCAGCTGG AATTCCTGAA GGAGGTGCAG CAGTCCCTGG GGAAGTCCGA GGTGCTGGTT 
TTCCTGCAAG CCCTCCTGGC GTCCAAGAAG CATAAGGGGG AGCAGGAGGC CACGACGCTG 
CTAAAGGAGG CCGCTGAGCT GCACTTCTCC AGCATGCAGG CCCTCCCCAT GAGCTCCGAA 
TACTTGGAAC GGCTCGACCC CATCTTCCTG GTCTGCATCG CCAAGGAATA CCTGGTCTTC 
TGCCCCAAGC AGCCCCGGTC TCCAGGCCAG ATCGTGTCTC CTCTTCTTAA GCAAGTCACT 
GCGATCTTGA ATCCTGTAGT CAAAGTGGCA CCAGCCCTGA TTGAGCCGCT GTATGTGATG 
GCCCAGGTCA AGTATCTCTC AGGGGAGTTA GAGCATGCCC AGAGCACCCT GCAGCGTTGC 
CTGGAGCTGG ACCCCACCTC CGTGGACGCC CACCTCCTCA TGGCTCAGAT CTACCTGGCT 
CAGGACAACT TTGCCATGTG CTCCCACTCC TTGGAGCTGG GCGTCAGCCA CAACTTCCAG 
GTCCGAGACC ACCCGCTTTA CCACTTCATC AAGGCCAAGG CCCTCAACAA GTCTGGGGAC 
TATCCAGAAG CTATAAAGGC ACTGAAGATG ATCATAAAAT TACCAACTCT GAGGACGGAA 
GAGAACAAGA AGTTCCGTGG GCCCTGCGTG CGCCCCAGCG AGCGGGTGTC CATCTTACTG 
GAGCTGGCAG ATGCCCTCCG GATGAATGGA GAGCTGCACG AGGCCACCAA GGTCATGCAG 
GATGCCATCA ACGAGTTCAG CGGCACGCCG GAGGAGATGC GCATCACCAT CGCCAATGTG 
GACTTGGCCC TGAGCAAGGG CAACGTGGAC CTGGCTCTGA GCGTGCTGCA GAGCATCACA 
CCCAAGCAGC CCTGCTACAT GGAAGCCAAG GAGAAGATGG CCAGCATCTA CCTGCAGACC 
CGCAAGGACG CCCGCCTCTA TATCGGGTGC TACCGGGAGC TCTGTGAACA TCTGCCTGGC 
CCCCACACCA GTCTGCTTCT GGGTGATGCT TTCATGAACA TTCAGGAGCC AGAGAAGGCC 
CTGGAGGTCT ATGATGAGGC CTACAGAAAG AACCCACACG ACACATCCCT GATCAGCCGG 
ATCGGGCAGG CTTATGTCAA GACCCACCAG TACACCAAGG CAATTAATTA TTATGAGGCT 
GCCCAGAAGA TTAGCGGACA GGACTTTGTG TGCTGCGATC TGGCCGAACT TCTCCTGAAG 
TTAAAGAAGT TCAACAAAGC AGAAAAAGTT TTGAAGCAGG CACTGGATCA TGACTTTGTC 
AAAGACATCC CATCCATGAT GAATGATGTC AAGTGCTTAC TTTTGCTGGC CAAGGTTTAC 
AAGAGTCATA AAAAAGAAGA TGTGACGGAA ACTTTGAACA AGGCCATGGA CCTCCAGTCT 
CGGATCCTGA AGCGTGTTCC CCTGGAGCGA CCAGAAATGA TCCCCTCCCA GAAGCAACTG 
GCAGCCTCCA TCTGCATCCA GTTTGGGGAA TACTACCTGG CAGAGAAGGA GTATGCCAAG 
GCAGTGCAGT CTTATAAAGA TGCCCTCTCC TACACGCCTA TTAACAATAA GGTGGTGCTG 
GAGCTGGCGA GGCTCTACCT GCTGCAGGGA CACCTGGACC TGTGTGAGCA GCACTGTGCC 
GACCTCCTGC AGACTGAGAA GAACCACGAG GCAGCCTCCG TGGTGATGGC TGACCTGATG 
TTTAGAAGAC AGAAATACGA GGCTGCCATC AACCTCTACC ACCAAGTCCT GGAGAAAGCA 
CCAGACAATT TTCCTGTATT GAACAAACTA ATCGATCTGC TACGAAGAAG TGGCAAACTT 
GAGGCCGCCC CTGCCGTCTT TGAATTGGCC GAGAAGATGT CCAGCCGGGT GCCTTTGGAG 
CCAGGGTTCA ACTTCTGCAG AGGCATCTAC TGCTGGCACA TAGGACAGCC CAATGAAGCC 
CTGAAGTTCC TGAACAAAGC GCGCAAGGAC AGCGCTTGGG GCCAGAGCGC CACCTACCAC 
ATGGTGCAGA TCTGTCTGAA CCCAGACAAC GAGATCGTGG GCGGAGAGGC TTTCGAGAAC 
CTGGTGACCG AGAGCAACCC CAGTGCCCGC AGCTTCAGCA GCAGGAAGGA GTTGGAGCAA 
CATGGCGTGC GCACCGCCGA GAAGCTGCTG CAGGAGTTCT ACCCACACTC GGCCGGGGGC 
CATACCCAGC TGCGGCTGCT GCAGAGCCTG TGCCTGCTGG CGACCAGGGA GAAGGCCAAC 
GTGGAAGTGG CGCTGAGCAC CTTCGTTGAG ATGGCCCAGG CTGAGAAGGA CAGCGTCCCC 
GCCCTGCTGG CCATGGCGCA GGCCTGCTCC CTGCTGAAGC AGGTCCCCAA GGCACGCACG 
CAGCTGAAGC GCCTGGCCAA GGCCCCGTGG GCACTGGCTG AGGCCGATGA CCTGGAGAGG 
AGCTGGCTCC TGCTGGCCGA TATCTACTGC CAGGGCGGCA AGTTTGACCT GGCCTTGGAG 
CTGCTGCAGC GCTGCGTGAA ATACAACAAG TCCTGCTGTA AGGCCTACGA GTACATGGGC 
TTCATCATGG AAAGGGAGCG GTCATACAAG GACGCGGCCT CCAACTACGA GCTGGCCTGG 
CAGTACAGCC GTCACACCAG CCCCGCCATC GGTACAGTCG TCGGGCAGGC ACGCGCTTCA 
GGCTCGCTTT CAACTACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC21A ( XM_025272322.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128107.1 Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X8, mRNA.

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CloneID	OBu57658
Clone ID Related Accession (Same CDS sequence)	XM_025272322.1
Accession Version	XM_025272322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	tetratricopeptide repeat protein 21A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCA TTTATGCTCA CAAGTGCTGT GAAACCATTG ACCGAGAAGC AATTCAGGAG 
CTTGAGAGCA GCCTGAAAGA AGTCCGCAAG ACAGCCAGTG GGACTGCCCT GTACTATGCT 
GGCCTCTTCC TCTGGCTCAT GGGCCTCCAC GACAAGGCCA AGGAATACAT TGACCGCACG 
TTGAAGATCT CCAGCAGCTC CAGAGAGGGC TATGTGCTCA GAGGCTGGGT GGACCTCAGC 
TCAGACAAAC CCCACACGGT GAAGAAGTCC ATCAAGTACC TGGAGCATGG CATTCAGGAC 
ACCAAAGACG TCCTGGGACT AATGGGAAAG GTGATGTACT TCATGATGCT GCAGAACTAC 
TCGGGGGCCC TTGAGGTGGT GAACCAGATC ACTGTCACCT GCGGGAGCTT CCTGCCTGCC 
TTGGTCCTAA AGATGCGGCT GTTCCTGGCT CGGCAGGACT GGGAGCAGAC GGTAGAAATG 
GGACACAGAA TCCTCGAAAA AGACAAAAGC AATATCGATG CCTGCCAAAT TCTAGCTGTG 
CATGAGCTCG CAAGAGAAGG AAACATGACC GTGGCTGCCG AGCACGTTAG GAATCTGATT 
AAGGCACTGG AAACCAGAGA GCCCCAAAAT CCAAGCCTCC ATCTTAAAAA AATTCTTGTG 
GTGAGCAGAC TGTGTGGGAG GCACCAGGGG ATTCTGCAGC TGGTGTGTAG CTTCATCGAA 
CGAGCCTTCA TGGCCACATC CTCCGACGCC CACATGGCCA CCGAGCTGGG CTACCTCTTC 
ATCCTGCAGG ACCAAGTAAA GGAGGCGTCT CTGTGGTACT CGAAGGCCAT GAAGCTGGAC 
GAGAGCAGCG TGGCTGCTTT GACAGGGATC ATCTGGTGCC AGATCTTAGA CGGCCACCTG 
GAGGAGGCCG AACACCAGCT GGAATTCCTG AAGGAGGTGC AGCAGTCCCT GGGGAAGTCC 
GAGGTGCTGG TTTTCCTGCA AGCCCTCCTG GCGTCCAAGA AGCATAAGGG GGAGCAGGAG 
GCCACGACGC TGCTAAAGGA GGCCGCTGAG CTGCACTTCT CCAGCATGCA GGCCCTCCCC 
ATGAGCTCCG AATACTTGGA ACGGCTCGAC CCCATCTTCC TGGTCTGCAT CGCCAAGGAA 
TACCTGGTCT TCTGCCCCAA GCAGCCCCGG TCTCCAGGCC AGATCGTGTC TCCTCTTCTT 
AAGCAAGTCA CTGCGATCTT GAATCCTGTA GTCAAAGTGG CACCAGCCCT GATTGAGCCG 
CTGTATGTGA TGGCCCAGGT CAAGTATCTC TCAGGGGAGT TAGAGCATGC CCAGAGCACC 
CTGCAGCGTT GCCTGGAGCT GGACCCCACC TCCGTGGACG CCCACCTCCT CATGGCTCAG 
ATCTACCTGG CTCAGGACAA CTTTGCCATG TGCTCCCACT CCTTGGAGCT GGGCGTCAGC 
CACAACTTCC AGGTCCGAGA CCACCCGCTT TACCACTTCA TCAAGGCCAA GGCCCTCAAC 
AAGTCTGGGG ACTATCCAGA AGCTATAAAG GCACTGAAGA TGATCATAAA ATTACCAACT 
CTGAGGACGG AAGAGAACAA GAAGTTCCGT GGGCCCTGCG TGCGCCCCAG CGAGCGGGTG 
TCCATCTTAC TGGAGCTGGC AGATGCCCTC CGGATGAATG GAGAGCTGCA CGAGGCCACC 
AAGGTCATGC AGGATGCCAT CAACGAGTTC AGCGGCACGC CGGAGGAGAT GCGCATCACC 
ATCGCCAATG TGGACTTGGC CCTGAGCAAG GGCAACGTGG ACCTGGCTCT GAGCGTGCTG 
CAGAGCATCA CACCCAAGCA GCCCTGCTAC ATGGAAGCCA AGGAGAAGAT GGCCAGCATC 
TACCTGCAGA CCCGCAAGGA CGCCCGCCTC TATATCGGGT GCTACCGGGA GCTCTGTGAA 
CATCTGCCTG GCCCCCACAC CAGTCTGCTT CTGGGTGATG CTTTCATGAA CATTCAGGAG 
CCAGAGAAGG CCCTGGAGGT CTATGATGAG GCCTACAGAA AGAACCCACA CGACACATCC 
CTGATCAGCC GGATCGGGCA GGCTTATGTC AAGACCCACC AGTACACCAA GGCAATTAAT 
TATTATGAGG CTGCCCAGAA GATTAGCGGA CAGGACTTTG TGTGCTGCGA TCTGGCCGAA 
CTTCTCCTGA AGTTAAAGAA GTTCAACAAA GCAGAAAAAG TTTTGAAGCA GGCACTGGAT 
CATGACTTTG TCAAAGACAT CCCATCCATG ATGAATGATG TCAAGTGCTT ACTTTTGCTG 
GCCAAGGTTT ACAAGAGTCA TAAAAAAGAA GATGTGACGG AAACTTTGAA CAAGGCCATG 
GACCTCCAGT CTCGGATCCT GAAGCGTGTT CCCCTGGAGC GACCAGAAAT GATCCCCTCC 
CAGAAGCAAC TGGCAGCCTC CATCTGCATC CAGTTTGGGG AATACTACCT GGCAGAGAAG 
GAGTATGCCA AGGCAGTGCA GTCTTATAAA GATGCCCTCT CCTACACGCC TATTAACAAT 
AAGGTGGTGC TGGAGCTGGC GAGGCTCTAC CTGCTGCAGG GACACCTGGA CCTGTGTGAG 
CAGCACTGTG CCGACCTCCT GCAGACTGAG AAGAACCACG AGGCAGCCTC CGTGGTGATG 
GCTGACCTGA TGTTTAGAAG ACAGAAATAC GAGGCTGCCA TCAACCTCTA CCACCAAGTC 
CTGGAGAAAG CACCAGACAA TTTTCCTGTA TTGAACAAAC TAATCGATCT GCTACGAAGA 
AGTGGCAAAC TTGAGGCCGC CCCTGCCGTC TTTGAATTGG CCGAGAAGAT GTCCAGCCGG 
GTGCCTTTGG AGCCAGGGTT CAACTTCTGC AGAGGCATCT ACTGCTGGCA CATAGGACAG 
CCCAATGAAG CCCTGAAGTT CCTGAACAAA GCGCGCAAGG ACAGCGCTTG GGGCCAGAGC 
GCCACCTACC ACATGGTGCA GATCTGTCTG AACCCAGACA ACGAGATCGT GGGCGGAGAG 
GCTTTCGAGA ACCTGGTGAC CGAGAGCAAC CCCAGTGCCC GCAGCTTCAG CAGCAGGAAG 
GAGTTGGAGC AACATGGCGT GCGCACCGCC GAGAAGCTGC TGCAGGAGTT CTACCCACAC 
TCGGCCGGGG GCCATACCCA GCTGCGGCTG CTGCAGAGCC TGTGCCTGCT GGCGACCAGG 
GAGAAGGCCA ACGTGGAAGT GGCGCTGAGC ACCTTCGTTG AGATGGCCCA GGCTGAGAAG 
GACAGCGTCC CCGCCCTGCT GGCCATGGCG CAGGCCTGCT CCCTGCTGAA GCAGGTCCCC 
AAGGCACGCA CGCAGCTGAA GCGCCTGGCC AAGGCCCCGT GGGCACTGGC TGAGGCCGAT 
GACCTGGAGA GGAGCTGGCT CCTGCTGGCC GATATCTACT GCCAGGGCGG CAAGTTTGAC 
CTGGCCTTGG AGCTGCTGCA GCGCTGCGTG AAATACAACA AGTCCTGCTG TAAGGCCTAC 
GAGTACATGG GCTTCATCAT GGAAAGGGAG CGGTCATACA AGGACGCGGC CTCCAACTAC 
GAGCTGGCCT GGCAGTACAG CCGTCACACC AGCCCCGCCA TCGGCTTCAG GCTCGCTTTC 
AACTACTTGA AAGACAAGAG ATTCGTGGAA GCCATTGAAG TCTGCCATGA TGTCCTCAGG 
GAGCACCCCA ACTACCCTAA AATCAGAGAA GAAATTCTGG CAAAGGCCCA AGGGTCTCTG 
AGGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128107.1
CDS	197..3925
Translation	MALIYAHKCCETIDREAIQELESSLKEVRKTASGTALYYAGLFLWLMGLHDKAKEYIDRTLKISSSSREGYVLRGWVDLSSDKPHTVKKSIKYLEHGIQDTKDVLGLMGKVMYFMMLQNYSGALEVVNQITVTCGSFLPALVLKMRLFLARQDWEQTVEMGHRILEKDKSNIDACQILAVHELAREGNMTVAAEHVRNLIKALETREPQNPSLHLKKILVVSRLCGRHQGILQLVCSFIERAFMATSSDAHMATELGYLFILQDQVKEASLWYSKAMKLDESSVAALTGIIWCQILDGHLEEAEHQLEFLKEVQQSLGKSEVLVFLQALLASKKHKGEQEATTLLKEAAELHFSSMQALPMSSEYLERLDPIFLVCIAKEYLVFCPKQPRSPGQIVSPLLKQVTAILNPVVKVAPALIEPLYVMAQVKYLSGELEHAQSTLQRCLELDPTSVDAHLLMAQIYLAQDNFAMCSHSLELGVSHNFQVRDHPLYHFIKAKALNKSGDYPEAIKALKMIIKLPTLRTEENKKFRGPCVRPSERVSILLELADALRMNGELHEATKVMQDAINEFSGTPEEMRITIANVDLALSKGNVDLALSVLQSITPKQPCYMEAKEKMASIYLQTRKDARLYIGCYRELCEHLPGPHTSLLLGDAFMNIQEPEKALEVYDEAYRKNPHDTSLISRIGQAYVKTHQYTKAINYYEAAQKISGQDFVCCDLAELLLKLKKFNKAEKVLKQALDHDFVKDIPSMMNDVKCLLLLAKVYKSHKKEDVTETLNKAMDLQSRILKRVPLERPEMIPSQKQLAASICIQFGEYYLAEKEYAKAVQSYKDALSYTPINNKVVLELARLYLLQGHLDLCEQHCADLLQTEKNHEAASVVMADLMFRRQKYEAAINLYHQVLEKAPDNFPVLNKLIDLLRRSGKLEAAPAVFELAEKMSSRVPLEPGFNFCRGIYCWHIGQPNEALKFLNKARKDSAWGQSATYHMVQICLNPDNEIVGGEAFENLVTESNPSARSFSSRKELEQHGVRTAEKLLQEFYPHSAGGHTQLRLLQSLCLLATREKANVEVALSTFVEMAQAEKDSVPALLAMAQACSLLKQVPKARTQLKRLAKAPWALAEADDLERSWLLLADIYCQGGKFDLALELLQRCVKYNKSCCKAYEYMGFIMERERSYKDAASNYELAWQYSRHTSPAIGFRLAFNYLKDKRFVEAIEVCHDVLREHPNYPKIREEILAKAQGSLRP

Target ORF information:

RefSeq Version	XM_025272322.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tetratricopeptide repeat domain 21A (TTC21A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025272322.1

ORF Insert Sequence:

ATGGCCCTCA TTTATGCTCA CAAGTGCTGT GAAACCATTG ACCGAGAAGC AATTCAGGAG 
CTTGAGAGCA GCCTGAAAGA AGTCCGCAAG ACAGCCAGTG GGACTGCCCT GTACTATGCT 
GGCCTCTTCC TCTGGCTCAT GGGCCTCCAC GACAAGGCCA AGGAATACAT TGACCGCACG 
TTGAAGATCT CCAGCAGCTC CAGAGAGGGC TATGTGCTCA GAGGCTGGGT GGACCTCAGC 
TCAGACAAAC CCCACACGGT GAAGAAGTCC ATCAAGTACC TGGAGCATGG CATTCAGGAC 
ACCAAAGACG TCCTGGGACT AATGGGAAAG GTGATGTACT TCATGATGCT GCAGAACTAC 
TCGGGGGCCC TTGAGGTGGT GAACCAGATC ACTGTCACCT GCGGGAGCTT CCTGCCTGCC 
TTGGTCCTAA AGATGCGGCT GTTCCTGGCT CGGCAGGACT GGGAGCAGAC GGTAGAAATG 
GGACACAGAA TCCTCGAAAA AGACAAAAGC AATATCGATG CCTGCCAAAT TCTAGCTGTG 
CATGAGCTCG CAAGAGAAGG AAACATGACC GTGGCTGCCG AGCACGTTAG GAATCTGATT 
AAGGCACTGG AAACCAGAGA GCCCCAAAAT CCAAGCCTCC ATCTTAAAAA AATTCTTGTG 
GTGAGCAGAC TGTGTGGGAG GCACCAGGGG ATTCTGCAGC TGGTGTGTAG CTTCATCGAA 
CGAGCCTTCA TGGCCACATC CTCCGACGCC CACATGGCCA CCGAGCTGGG CTACCTCTTC 
ATCCTGCAGG ACCAAGTAAA GGAGGCGTCT CTGTGGTACT CGAAGGCCAT GAAGCTGGAC 
GAGAGCAGCG TGGCTGCTTT GACAGGGATC ATCTGGTGCC AGATCTTAGA CGGCCACCTG 
GAGGAGGCCG AACACCAGCT GGAATTCCTG AAGGAGGTGC AGCAGTCCCT GGGGAAGTCC 
GAGGTGCTGG TTTTCCTGCA AGCCCTCCTG GCGTCCAAGA AGCATAAGGG GGAGCAGGAG 
GCCACGACGC TGCTAAAGGA GGCCGCTGAG CTGCACTTCT CCAGCATGCA GGCCCTCCCC 
ATGAGCTCCG AATACTTGGA ACGGCTCGAC CCCATCTTCC TGGTCTGCAT CGCCAAGGAA 
TACCTGGTCT TCTGCCCCAA GCAGCCCCGG TCTCCAGGCC AGATCGTGTC TCCTCTTCTT 
AAGCAAGTCA CTGCGATCTT GAATCCTGTA GTCAAAGTGG CACCAGCCCT GATTGAGCCG 
CTGTATGTGA TGGCCCAGGT CAAGTATCTC TCAGGGGAGT TAGAGCATGC CCAGAGCACC 
CTGCAGCGTT GCCTGGAGCT GGACCCCACC TCCGTGGACG CCCACCTCCT CATGGCTCAG 
ATCTACCTGG CTCAGGACAA CTTTGCCATG TGCTCCCACT CCTTGGAGCT GGGCGTCAGC 
CACAACTTCC AGGTCCGAGA CCACCCGCTT TACCACTTCA TCAAGGCCAA GGCCCTCAAC 
AAGTCTGGGG ACTATCCAGA AGCTATAAAG GCACTGAAGA TGATCATAAA ATTACCAACT 
CTGAGGACGG AAGAGAACAA GAAGTTCCGT GGGCCCTGCG TGCGCCCCAG CGAGCGGGTG 
TCCATCTTAC TGGAGCTGGC AGATGCCCTC CGGATGAATG GAGAGCTGCA CGAGGCCACC 
AAGGTCATGC AGGATGCCAT CAACGAGTTC AGCGGCACGC CGGAGGAGAT GCGCATCACC 
ATCGCCAATG TGGACTTGGC CCTGAGCAAG GGCAACGTGG ACCTGGCTCT GAGCGTGCTG 
CAGAGCATCA CACCCAAGCA GCCCTGCTAC ATGGAAGCCA AGGAGAAGAT GGCCAGCATC 
TACCTGCAGA CCCGCAAGGA CGCCCGCCTC TATATCGGGT GCTACCGGGA GCTCTGTGAA 
CATCTGCCTG GCCCCCACAC CAGTCTGCTT CTGGGTGATG CTTTCATGAA CATTCAGGAG 
CCAGAGAAGG CCCTGGAGGT CTATGATGAG GCCTACAGAA AGAACCCACA CGACACATCC 
CTGATCAGCC GGATCGGGCA GGCTTATGTC AAGACCCACC AGTACACCAA GGCAATTAAT 
TATTATGAGG CTGCCCAGAA GATTAGCGGA CAGGACTTTG TGTGCTGCGA TCTGGCCGAA 
CTTCTCCTGA AGTTAAAGAA GTTCAACAAA GCAGAAAAAG TTTTGAAGCA GGCACTGGAT 
CATGACTTTG TCAAAGACAT CCCATCCATG ATGAATGATG TCAAGTGCTT ACTTTTGCTG 
GCCAAGGTTT ACAAGAGTCA TAAAAAAGAA GATGTGACGG AAACTTTGAA CAAGGCCATG 
GACCTCCAGT CTCGGATCCT GAAGCGTGTT CCCCTGGAGC GACCAGAAAT GATCCCCTCC 
CAGAAGCAAC TGGCAGCCTC CATCTGCATC CAGTTTGGGG AATACTACCT GGCAGAGAAG 
GAGTATGCCA AGGCAGTGCA GTCTTATAAA GATGCCCTCT CCTACACGCC TATTAACAAT 
AAGGTGGTGC TGGAGCTGGC GAGGCTCTAC CTGCTGCAGG GACACCTGGA CCTGTGTGAG 
CAGCACTGTG CCGACCTCCT GCAGACTGAG AAGAACCACG AGGCAGCCTC CGTGGTGATG 
GCTGACCTGA TGTTTAGAAG ACAGAAATAC GAGGCTGCCA TCAACCTCTA CCACCAAGTC 
CTGGAGAAAG CACCAGACAA TTTTCCTGTA TTGAACAAAC TAATCGATCT GCTACGAAGA 
AGTGGCAAAC TTGAGGCCGC CCCTGCCGTC TTTGAATTGG CCGAGAAGAT GTCCAGCCGG 
GTGCCTTTGG AGCCAGGGTT CAACTTCTGC AGAGGCATCT ACTGCTGGCA CATAGGACAG 
CCCAATGAAG CCCTGAAGTT CCTGAACAAA GCGCGCAAGG ACAGCGCTTG GGGCCAGAGC 
GCCACCTACC ACATGGTGCA GATCTGTCTG AACCCAGACA ACGAGATCGT GGGCGGAGAG 
GCTTTCGAGA ACCTGGTGAC CGAGAGCAAC CCCAGTGCCC GCAGCTTCAG CAGCAGGAAG 
GAGTTGGAGC AACATGGCGT GCGCACCGCC GAGAAGCTGC TGCAGGAGTT CTACCCACAC 
TCGGCCGGGG GCCATACCCA GCTGCGGCTG CTGCAGAGCC TGTGCCTGCT GGCGACCAGG 
GAGAAGGCCA ACGTGGAAGT GGCGCTGAGC ACCTTCGTTG AGATGGCCCA GGCTGAGAAG 
GACAGCGTCC CCGCCCTGCT GGCCATGGCG CAGGCCTGCT CCCTGCTGAA GCAGGTCCCC 
AAGGCACGCA CGCAGCTGAA GCGCCTGGCC AAGGCCCCGT GGGCACTGGC TGAGGCCGAT 
GACCTGGAGA GGAGCTGGCT CCTGCTGGCC GATATCTACT GCCAGGGCGG CAAGTTTGAC 
CTGGCCTTGG AGCTGCTGCA GCGCTGCGTG AAATACAACA AGTCCTGCTG TAAGGCCTAC 
GAGTACATGG GCTTCATCAT GGAAAGGGAG CGGTCATACA AGGACGCGGC CTCCAACTAC 
GAGCTGGCCT GGCAGTACAG CCGTCACACC AGCCCCGCCA TCGGCTTCAG GCTCGCTTTC 
AACTACTTGA AAGACAAGAG ATTCGTGGAA GCCATTGAAG TCTGCCATGA TGTCCTCAGG 
GAGCACCCCA ACTACCCTAA AATCAGAGAA GAAATTCTGG CAAAGGCCCA AGGGTCTCTG 
AGGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TTC21A cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム