NHSL2 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	NHSL2
Entrez Gene ID	102398431
Full Name	NHS like 2
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006070033.2	XP_006070095.2	NHS-like protein 2 isoform X1
XM_006070034.2	XP_006070096.2	NHS-like protein 2 isoform X2
XM_006070034.1	XP_006070096.1	NHS-like protein 2 isoform X2
XM_006070033.1	XP_006070095.1	NHS-like protein 2 isoform X1
XM_006070035.1	XP_006070097.1	NHS-like protein 2 isoform X3

Pathways from BioSystems
Gene Ontology

The following NHSL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NHSL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu24720	XM_006070035.1 Latest version!	Bubalus bubalis NHS-like 2 (NHSL2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu73920	XM_006070033.2 Latest version!	Bubalus bubalis NHS-like protein 2 (LOC102398431), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBu73921	XM_006070034.2 Latest version!	Bubalus bubalis NHS-like protein 2 (LOC102398431), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBu24718	XM_006070033.1 Latest version!	Bubalus bubalis NHS-like 2 (NHSL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu24719	XM_006070034.1 Latest version!	Bubalus bubalis NHS-like 2 (NHSL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NHSL2 ( XM_006070035.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006070097.1 Bubalus bubalis NHS-like 2 (NHSL2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu24720
Clone ID Related Accession (Same CDS sequence)	XM_006070035.1
Accession Version	XM_006070035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	NHS-like protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785381.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CCGAGGCTAT CACCCTCTTC TGGAGCCGGG GAGGTCACAG CAACAGCCCA 
GCAGGCAGTG TGGCCCACTC CACCACCTCA GACATCAGGC CCAGCCATTC AGTTCCAGAA 
AGTGTTCATG GAAGAGTTGC ACTTGGTCAG GAAGCTCGGT TCCCAAATCT CACCTCACCA 
GTACTAAGAA ATCCTTCTAG TCATCCGGAA GAACCTCCCC AGGCATGGGG TGCCACAAAA 
CCCCCTGGAA TGGAGAGCAT GGGGATGGTG TACAGCATTC CTGGTTCTTG CAATGGACCA 
ACAGAATCGA CATTCTCTGC TTCCTGGAAG GGAGATGCTT TTACCTATGT GACTCCAAGT 
GCCACCGCTC AGAGCAGTCA AGTCAATGAA AATGGAAAAA ATCCTTCCTC TGGGAATTCT 
TGGGTCTCTC TGCACACACT GCCACCTCTT GTTCCTAAGG AGGCTGCTAC CCTCTTTGTC 
ACTCGTGATA TCCCAGCAGG GTGCAGTGGG CCTGCTGGCT ACTCTCAGCA TCCTACTCAA 
CGAAGCCAAG TATCTGAGCG ACCCTCCAAG ATTGGCCTTC TGACCGGTGG TACCTCAAGG 
CTGGAGACAG GCCCAGGTGG GGCCAGCAGG TTCCGGGAGC GGTCACTGTC TGTACCCACA 
GATACAGGTA CCATGGATGT GGACTATGAG GAGGAGCAGA AGGCCAGTGA AGCCTGTGCC 
CTACCTTATG CCAGTACGAG TTCTGAGGGC AGTAACAGTG CTGACAACAT TGCCTCCCTC 
AGTGCCCAAC AGGAGGCCCA TCACAGAAGG CAGAGGTCCA AGAGCATCTC ACTCAAGAAG 
GCCAAGAAGA AGCCTTCCCC TCCGACTCGC AGTGTCTCAC TGGTCAAAGA TGAACCAGTC 
CTCTTGCCGG AAGGTGGGTC AGTACTACCC AAGGACCAGA GGCCCAGGAG CCTTTGTCTC 
TCCTTGGAAC ACCAAGGACA TCACTCATCC CACCCAGATA CTCAGGGTCA CCCAGCTGTG 
CCAACCTTCA AAGACCCAGA AGGTACACAA TTCGCCCATC ACTGGTATCT TACTGACTGG 
AAGTCTGGTG ACACCTACCA GTCCTTGTCC AGCTCCAGCA CCGCCACTGG CACCACAGTC 
ATTGAGTGCA CCCAAGTTCA GGGCAGCTCA GAGTCTCTTG CCTCCCCTTC CACTTCCAGA 
GCCACGACGC CTTCCCAGCT TTCCATCGAG GTGGAGGCCA GGGAGGTATC CTCTCCAGGA 
AGGCCTACTG GGCTGATGTC ACCCTCCAGT GGATACTCCA GCCAGTCAGA GACGCCAACA 
CCCACTGTCT CCATGTCCTT GACCCTGGGC CACTTGCCCC CTCCAAGCAG CAGTGTCCGG 
GTGCGTCCAG TGGTACCTGA GAGGAAGTCA TCACTACCCC CAACATCACC AATGGAAAAA 
ATGTCCAAGT CACGGCTATC GTTTGACCTA CCATTGACCT CTTCAACCAA CCTGGATCTG 
TCTGGGATGA GTATCTCAAT CCGAAGCAAA AACAAGGTGA GCCAGCATCA CTCAGACACA 
AATTTGGGGG CCAAGCTGGC CCAGAAAACT AGCCCCAACC AGCCAATCAT GCCTATGGTT 
ACTCAGTCTG ACTTACGTTC TGTTCGCCTG AGGTCAGTCA GCAAGTCTGA GCTGGAAGAT 
GACATTGAGA GCCCTGACTA TGCTGAGGAA TCAGGAGCTG AAGTCTTCAC CTTGCCAGAG 
AGGAAGATGA AACCTCCCAT AGCCGAGAAG CCCCCTCTGG CCCGAAGGCC TGCAAGCTTA 
GTCCACAAGC CACCATCTGT CCCCGAGGAG TACCCATTAA CTTCGCCTAC CTTGGCTATG 
ACTCCCAAGA GCTCAATTCA GCACATAAGG CCACTCCCTC AAGATATCTA CACAGTGGTG 
CGGAAATCAA AGTCCTCCAG CTTCCCTGAG AGCAGAAGCC CAGGGGAGTC CACAGCACCC 
TCATCTCTTG TTTTCACGCC TTTTGCCAGT TCCTCTGGTG CTTTCTTCTC AGGAACACAG 
CAACCTCCCC AGGGAAGTAT GGAGGATGAG GGCCCTAAGG GGAGAGCCCT GCCTGAAAGA 
ATTAGCCTCC AGAGCCAAGA AGAAGCTGAG AAAAAGAAAG GCAAGATTCC ACCTCCTGTA 
CCAAAAAAGC CCAGCGTGCT GTACCTGCCT CTCACTTCAC CCACGGCTCA AGCGGAGGCC 
TATGTGACTG AACCAAGACT GCCCCTCAGC CCCATCATCA CCCTGGAGGA AGAGGCCAAG 
TGTCTCCCAA CTGATGATCA CCTGCCATCT GCTGGTACAA GGATGACCTC AAGGCCACAG 
TCCAACAGCG AAAGGGAAGC AAGTCCTCCA GGGAGTTTGA TGGAACCAAG CACCGAAGAA 
AAAAGTGTAA TCAGTGATAA AACAGCCGAA TGGATTGCAG AAGATGATGA TGATGTGTTT 
GTGGCTTCAC GTACAACTGA AGATTTATTT ACTGTGATCC ACAGGTCCAA AAGAAAGCTG 
CTTGGCTGGA AGGAACCTGG TGAGACCTTT GCTGGCAGCA GCAGACCAAG CTCCCACTCA 
CCAGTAAGGA ACCCACCTGA GTCTCCGGTC AGTGAGTTGG CTGCCTCTGC AGGGTCAAGC 
GGCAGTGCCA ACCTAGATGC TGGCAGAAAT GATGATTTCA AGGCCTTGCT ACAGAAGAAG 
GGAAGCAAGG CAACTCCAAG GTCCCGCCCC TCAGCAGCCG AACTGCTGAA GACCACTAAC 
CCACTGGCTC GGCGAATTAT TGCACAATTT TCAAAAGACT ACAAAACCCC TGATAACCCC 
AGTACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006070097.1
CDS	398..3226
Translation	MERAEAITLFWSRGGHSNSPAGSVAHSTTSDIRPSHSVPESVHGRVALGQEARFPNLTSPVLRNPSSHPEEPPQAWGATKPPGMESMGMVYSIPGSCNGPTESTFSASWKGDAFTYVTPSATAQSSQVNENGKNPSSGNSWVSLHTLPPLVPKEAATLFVTRDIPAGCSGPAGYSQHPTQRSQVSERPSKIGLLTGGTSRLETGPGGASRFRERSLSVPTDTGTMDVDYEEEQKASEACALPYASTSSEGSNSADNIASLSAQQEAHHRRQRSKSISLKKAKKKPSPPTRSVSLVKDEPVLLPEGGSVLPKDQRPRSLCLSLEHQGHHSSHPDTQGHPAVPTFKDPEGTQFAHHWYLTDWKSGDTYQSLSSSSTATGTTVIECTQVQGSSESLASPSTSRATTPSQLSIEVEAREVSSPGRPTGLMSPSSGYSSQSETPTPTVSMSLTLGHLPPPSSSVRVRPVVPERKSSLPPTSPMEKMSKSRLSFDLPLTSSTNLDLSGMSISIRSKNKVSQHHSDTNLGAKLAQKTSPNQPIMPMVTQSDLRSVRLRSVSKSELEDDIESPDYAEESGAEVFTLPERKMKPPIAEKPPLARRPASLVHKPPSVPEEYPLTSPTLAMTPKSSIQHIRPLPQDIYTVVRKSKSSSFPESRSPGESTAPSSLVFTPFASSSGAFFSGTQQPPQGSMEDEGPKGRALPERISLQSQEEAEKKKGKIPPPVPKKPSVLYLPLTSPTAQAEAYVTEPRLPLSPIITLEEEAKCLPTDDHLPSAGTRMTSRPQSNSEREASPPGSLMEPSTEEKSVISDKTAEWIAEDDDDVFVASRTTEDLFTVIHRSKRKLLGWKEPGETFAGSSRPSSHSPVRNPPESPVSELAASAGSSGSANLDAGRNDDFKALLQKKGSKATPRSRPSAAELLKTTNPLARRIIAQFSKDYKTPDNPST

Target ORF information:

RefSeq Version	XM_006070035.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NHS-like 2 (NHSL2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006070035.1

ORF Insert Sequence:

ATGGAGAGAG CCGAGGCTAT CACCCTCTTC TGGAGCCGGG GAGGTCACAG CAACAGCCCA 
GCAGGCAGTG TGGCCCACTC CACCACCTCA GACATCAGGC CCAGCCATTC AGTTCCAGAA 
AGTGTTCATG GAAGAGTTGC ACTTGGTCAG GAAGCTCGGT TCCCAAATCT CACCTCACCA 
GTACTAAGAA ATCCTTCTAG TCATCCGGAA GAACCTCCCC AGGCATGGGG TGCCACAAAA 
CCCCCTGGAA TGGAGAGCAT GGGGATGGTG TACAGCATTC CTGGTTCTTG CAATGGACCA 
ACAGAATCGA CATTCTCTGC TTCCTGGAAG GGAGATGCTT TTACCTATGT GACTCCAAGT 
GCCACCGCTC AGAGCAGTCA AGTCAATGAA AATGGAAAAA ATCCTTCCTC TGGGAATTCT 
TGGGTCTCTC TGCACACACT GCCACCTCTT GTTCCTAAGG AGGCTGCTAC CCTCTTTGTC 
ACTCGTGATA TCCCAGCAGG GTGCAGTGGG CCTGCTGGCT ACTCTCAGCA TCCTACTCAA 
CGAAGCCAAG TATCTGAGCG ACCCTCCAAG ATTGGCCTTC TGACCGGTGG TACCTCAAGG 
CTGGAGACAG GCCCAGGTGG GGCCAGCAGG TTCCGGGAGC GGTCACTGTC TGTACCCACA 
GATACAGGTA CCATGGATGT GGACTATGAG GAGGAGCAGA AGGCCAGTGA AGCCTGTGCC 
CTACCTTATG CCAGTACGAG TTCTGAGGGC AGTAACAGTG CTGACAACAT TGCCTCCCTC 
AGTGCCCAAC AGGAGGCCCA TCACAGAAGG CAGAGGTCCA AGAGCATCTC ACTCAAGAAG 
GCCAAGAAGA AGCCTTCCCC TCCGACTCGC AGTGTCTCAC TGGTCAAAGA TGAACCAGTC 
CTCTTGCCGG AAGGTGGGTC AGTACTACCC AAGGACCAGA GGCCCAGGAG CCTTTGTCTC 
TCCTTGGAAC ACCAAGGACA TCACTCATCC CACCCAGATA CTCAGGGTCA CCCAGCTGTG 
CCAACCTTCA AAGACCCAGA AGGTACACAA TTCGCCCATC ACTGGTATCT TACTGACTGG 
AAGTCTGGTG ACACCTACCA GTCCTTGTCC AGCTCCAGCA CCGCCACTGG CACCACAGTC 
ATTGAGTGCA CCCAAGTTCA GGGCAGCTCA GAGTCTCTTG CCTCCCCTTC CACTTCCAGA 
GCCACGACGC CTTCCCAGCT TTCCATCGAG GTGGAGGCCA GGGAGGTATC CTCTCCAGGA 
AGGCCTACTG GGCTGATGTC ACCCTCCAGT GGATACTCCA GCCAGTCAGA GACGCCAACA 
CCCACTGTCT CCATGTCCTT GACCCTGGGC CACTTGCCCC CTCCAAGCAG CAGTGTCCGG 
GTGCGTCCAG TGGTACCTGA GAGGAAGTCA TCACTACCCC CAACATCACC AATGGAAAAA 
ATGTCCAAGT CACGGCTATC GTTTGACCTA CCATTGACCT CTTCAACCAA CCTGGATCTG 
TCTGGGATGA GTATCTCAAT CCGAAGCAAA AACAAGGTGA GCCAGCATCA CTCAGACACA 
AATTTGGGGG CCAAGCTGGC CCAGAAAACT AGCCCCAACC AGCCAATCAT GCCTATGGTT 
ACTCAGTCTG ACTTACGTTC TGTTCGCCTG AGGTCAGTCA GCAAGTCTGA GCTGGAAGAT 
GACATTGAGA GCCCTGACTA TGCTGAGGAA TCAGGAGCTG AAGTCTTCAC CTTGCCAGAG 
AGGAAGATGA AACCTCCCAT AGCCGAGAAG CCCCCTCTGG CCCGAAGGCC TGCAAGCTTA 
GTCCACAAGC CACCATCTGT CCCCGAGGAG TACCCATTAA CTTCGCCTAC CTTGGCTATG 
ACTCCCAAGA GCTCAATTCA GCACATAAGG CCACTCCCTC AAGATATCTA CACAGTGGTG 
CGGAAATCAA AGTCCTCCAG CTTCCCTGAG AGCAGAAGCC CAGGGGAGTC CACAGCACCC 
TCATCTCTTG TTTTCACGCC TTTTGCCAGT TCCTCTGGTG CTTTCTTCTC AGGAACACAG 
CAACCTCCCC AGGGAAGTAT GGAGGATGAG GGCCCTAAGG GGAGAGCCCT GCCTGAAAGA 
ATTAGCCTCC AGAGCCAAGA AGAAGCTGAG AAAAAGAAAG GCAAGATTCC ACCTCCTGTA 
CCAAAAAAGC CCAGCGTGCT GTACCTGCCT CTCACTTCAC CCACGGCTCA AGCGGAGGCC 
TATGTGACTG AACCAAGACT GCCCCTCAGC CCCATCATCA CCCTGGAGGA AGAGGCCAAG 
TGTCTCCCAA CTGATGATCA CCTGCCATCT GCTGGTACAA GGATGACCTC AAGGCCACAG 
TCCAACAGCG AAAGGGAAGC AAGTCCTCCA GGGAGTTTGA TGGAACCAAG CACCGAAGAA 
AAAAGTGTAA TCAGTGATAA AACAGCCGAA TGGATTGCAG AAGATGATGA TGATGTGTTT 
GTGGCTTCAC GTACAACTGA AGATTTATTT ACTGTGATCC ACAGGTCCAA AAGAAAGCTG 
CTTGGCTGGA AGGAACCTGG TGAGACCTTT GCTGGCAGCA GCAGACCAAG CTCCCACTCA 
CCAGTAAGGA ACCCACCTGA GTCTCCGGTC AGTGAGTTGG CTGCCTCTGC AGGGTCAAGC 
GGCAGTGCCA ACCTAGATGC TGGCAGAAAT GATGATTTCA AGGCCTTGCT ACAGAAGAAG 
GGAAGCAAGG CAACTCCAAG GTCCCGCCCC TCAGCAGCCG AACTGCTGAA GACCACTAAC 
CCACTGGCTC GGCGAATTAT TGCACAATTT TCAAAAGACT ACAAAACCCC TGATAACCCC 
AGTACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL2 ( XM_006070033.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006070095.2 Bubalus bubalis NHS-like protein 2 (LOC102398431), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu73920
Clone ID Related Accession (Same CDS sequence)	XM_006070033.2
Accession Version	XM_006070033.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	NHS-like protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006070033.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CCGAGGCTAT CACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG 
TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAGCTGC TTCGCGAGGC GCAGCTCAAT 
CTCCAGAGCC TGTTGCAAGA AGAATATGAG GAACAGTACT CGGAGGCCAG ACTTCTGGGG 
CAGACCTTCC GCTCTGCTGA TGAGGCCCCT GAGCCCACCC CCAGCCCAAG GCTCCAGTCT 
GCCAGGCGTC TGGAGTTTGT ACTGATGCCC ACAAAATGGC AGCTGAGCGA GGATGAGACT 
ACCATGCAGG GTGTGAGGGC CCCTGAGGCC TCCCTGAGCC TGCCTACCAC AGCCGACAAG 
CAAGCTGCCT GGAATAGCCC CTTTCCTCTG CCCATCCTAG AGGAGAAGCG GTGGCTTCAG 
TCTTGCCCCA CACACTCTGA CATCGTGCCC ATCAACGTCT CCGGGCAGCA GTTTGATAAA 
CATGCAAGTT TGCGACACTC GTTGTTTAAC ACAGAGACAG CCGTGAACCC CAAGTCCACC 
CTGAGGCGGA GGCGGACCAT TATTGGATTC TCTAACTATT CCCAGCGAGA CCAAGGTCAC 
AGCAACAGCC CAGCAGGCAG TGTGGCCCAC TCCACCACCT CAGACATCAG GCCCAGCCAT 
TCAGTTCCAG AAAGTGTTCA TGGAAGAGTT GCACTTGGTC AGGAAGCTCG GTTCCCAAAT 
CTCACCTCAC CAGTACTAAG AAATCCTTCT AGTCATCCGG AAGAACCTCC CCAGGCATGG 
GGTGCCACAA AACCCCCTGG AATGGAGAGC ATGGGGATGG TGTACAGCAT TCCTGGTTCT 
TGCAATGGAC CAACAGAATC GACATTCTCT GCTTCCTGGA AGGGAGATGC TTTTACCTAT 
GTGACTCCAA GTGCCACCGC TCAGAGCAGT CAAGTCAATG AAAATGGAAA AAATCCTTCC 
TCTGGGAATT CTTGGGTCTC TCTGCACACA CTGCCACCTC TTGTTCCTAA GGAGGCTGCT 
ACCCTCTTTG TCACTCGTGA TATCCCAGCA GGGTGCAGTG GGCCTGCTGG CTACTCTCAG 
CATCCTACTC AACGAAGCCA AGTATCTGAG CGACCCTCCA AGATTGGCCT TCTGACCGGT 
GGTACCTCAA GGCTGGAGAC AGGCCCAGGT GGGGCCAGCA GGTTCCGGGA GCGGTCACTG 
TCTGTACCCA CAGATACAGG TACCATGGAT GTGGACTATG AGGAGGAGCA GAAGGCCAGT 
GAAGCCTGTG CCCTACCTTA TGCCAGTACG AGTTCTGAGG GCAGTAACAG TGCTGACAAC 
ATTGCCTCCC TCAGTGCCCA ACAGGAGGCC CATCACAGAA GGCAGAGGTC CAAGAGCATC 
TCACTCAAGA AGGCCAAGAA GAAGCCTTCC CCTCCGACTC GCAGTGTCTC ACTGGTCAAA 
GATGAACCAG TCCTCTTGCC GGAAGGTGGG TCAGTACTAC CCAAGGACCA GAGGCCCAGG 
AGCCTTTGTC TCTCCTTGGA ACACCAAGGA CATCACTCAT CCCACCCAGA TACTCAGGGT 
CACCCAGCTG TGCCAACCTT CAAAGACCCA GAAGGTACAC AATTCGCCCA TCACTGGTAT 
CTTACTGACT GGAAGTCTGG TGACACCTAC CAGTCCTTGT CCAGCTCCAG CACCGCCACT 
GGCACCACAG TCATTGAGTG CACCCAAGTT CAGGGCAGCT CAGAGTCTCT TGCCTCCCCT 
TCCACTTCCA GAGCCACGAC GCCTTCCCAG CTTTCCATCG AGGTGGAGGC CAGGGAGGTA 
TCCTCTCCAG GAAGGCCTAC TGGGCTGATG TCACCCTCCA GTGGATACTC CAGCCAGTCA 
GAGACGCCAA CACCCACTGT CTCCATGTCC TTGACCCTGG GCCACTTGCC CCCTCCAAGC 
AGCAGTGTCC GGGTGCGTCC AGTGGTACCT GAGAGGAAGT CATCACTACC CCCAACATCA 
CCAATGGAAA AAATGTCCAA GTCACGGCTA TCAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006070095.2
CDS	6851..8929
Translation	MERAEAITLFWSRGAAANSGRENATATAHSRSSWRQPVNVFLSSGRPPSVEELLREAQLNLQSLLQEEYEEQYSEARLLGQTFRSADEAPEPTPSPRLQSARRLEFVLMPTKWQLSEDETTMQGVRAPEASLSLPTTADKQAAWNSPFPLPILEEKRWLQSCPTHSDIVPINVSGQQFDKHASLRHSLFNTETAVNPKSTLRRRRTIIGFSNYSQRDQGHSNSPAGSVAHSTTSDIRPSHSVPESVHGRVALGQEARFPNLTSPVLRNPSSHPEEPPQAWGATKPPGMESMGMVYSIPGSCNGPTESTFSASWKGDAFTYVTPSATAQSSQVNENGKNPSSGNSWVSLHTLPPLVPKEAATLFVTRDIPAGCSGPAGYSQHPTQRSQVSERPSKIGLLTGGTSRLETGPGGASRFRERSLSVPTDTGTMDVDYEEEQKASEACALPYASTSSEGSNSADNIASLSAQQEAHHRRQRSKSISLKKAKKKPSPPTRSVSLVKDEPVLLPEGGSVLPKDQRPRSLCLSLEHQGHHSSHPDTQGHPAVPTFKDPEGTQFAHHWYLTDWKSGDTYQSLSSSSTATGTTVIECTQVQGSSESLASPSTSRATTPSQLSIEVEAREVSSPGRPTGLMSPSSGYSSQSETPTPTVSMSLTLGHLPPPSSSVRVRPVVPERKSSLPPTSPMEKMSKSRLSV

Target ORF information:

RefSeq Version	XM_006070033.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NHS-like protein 2 (LOC102398431), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006070033.2

ORF Insert Sequence:

ATGGAGAGAG CCGAGGCTAT CACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG 
TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAGCTGC TTCGCGAGGC GCAGCTCAAT 
CTCCAGAGCC TGTTGCAAGA AGAATATGAG GAACAGTACT CGGAGGCCAG ACTTCTGGGG 
CAGACCTTCC GCTCTGCTGA TGAGGCCCCT GAGCCCACCC CCAGCCCAAG GCTCCAGTCT 
GCCAGGCGTC TGGAGTTTGT ACTGATGCCC ACAAAATGGC AGCTGAGCGA GGATGAGACT 
ACCATGCAGG GTGTGAGGGC CCCTGAGGCC TCCCTGAGCC TGCCTACCAC AGCCGACAAG 
CAAGCTGCCT GGAATAGCCC CTTTCCTCTG CCCATCCTAG AGGAGAAGCG GTGGCTTCAG 
TCTTGCCCCA CACACTCTGA CATCGTGCCC ATCAACGTCT CCGGGCAGCA GTTTGATAAA 
CATGCAAGTT TGCGACACTC GTTGTTTAAC ACAGAGACAG CCGTGAACCC CAAGTCCACC 
CTGAGGCGGA GGCGGACCAT TATTGGATTC TCTAACTATT CCCAGCGAGA CCAAGGTCAC 
AGCAACAGCC CAGCAGGCAG TGTGGCCCAC TCCACCACCT CAGACATCAG GCCCAGCCAT 
TCAGTTCCAG AAAGTGTTCA TGGAAGAGTT GCACTTGGTC AGGAAGCTCG GTTCCCAAAT 
CTCACCTCAC CAGTACTAAG AAATCCTTCT AGTCATCCGG AAGAACCTCC CCAGGCATGG 
GGTGCCACAA AACCCCCTGG AATGGAGAGC ATGGGGATGG TGTACAGCAT TCCTGGTTCT 
TGCAATGGAC CAACAGAATC GACATTCTCT GCTTCCTGGA AGGGAGATGC TTTTACCTAT 
GTGACTCCAA GTGCCACCGC TCAGAGCAGT CAAGTCAATG AAAATGGAAA AAATCCTTCC 
TCTGGGAATT CTTGGGTCTC TCTGCACACA CTGCCACCTC TTGTTCCTAA GGAGGCTGCT 
ACCCTCTTTG TCACTCGTGA TATCCCAGCA GGGTGCAGTG GGCCTGCTGG CTACTCTCAG 
CATCCTACTC AACGAAGCCA AGTATCTGAG CGACCCTCCA AGATTGGCCT TCTGACCGGT 
GGTACCTCAA GGCTGGAGAC AGGCCCAGGT GGGGCCAGCA GGTTCCGGGA GCGGTCACTG 
TCTGTACCCA CAGATACAGG TACCATGGAT GTGGACTATG AGGAGGAGCA GAAGGCCAGT 
GAAGCCTGTG CCCTACCTTA TGCCAGTACG AGTTCTGAGG GCAGTAACAG TGCTGACAAC 
ATTGCCTCCC TCAGTGCCCA ACAGGAGGCC CATCACAGAA GGCAGAGGTC CAAGAGCATC 
TCACTCAAGA AGGCCAAGAA GAAGCCTTCC CCTCCGACTC GCAGTGTCTC ACTGGTCAAA 
GATGAACCAG TCCTCTTGCC GGAAGGTGGG TCAGTACTAC CCAAGGACCA GAGGCCCAGG 
AGCCTTTGTC TCTCCTTGGA ACACCAAGGA CATCACTCAT CCCACCCAGA TACTCAGGGT 
CACCCAGCTG TGCCAACCTT CAAAGACCCA GAAGGTACAC AATTCGCCCA TCACTGGTAT 
CTTACTGACT GGAAGTCTGG TGACACCTAC CAGTCCTTGT CCAGCTCCAG CACCGCCACT 
GGCACCACAG TCATTGAGTG CACCCAAGTT CAGGGCAGCT CAGAGTCTCT TGCCTCCCCT 
TCCACTTCCA GAGCCACGAC GCCTTCCCAG CTTTCCATCG AGGTGGAGGC CAGGGAGGTA 
TCCTCTCCAG GAAGGCCTAC TGGGCTGATG TCACCCTCCA GTGGATACTC CAGCCAGTCA 
GAGACGCCAA CACCCACTGT CTCCATGTCC TTGACCCTGG GCCACTTGCC CCCTCCAAGC 
AGCAGTGTCC GGGTGCGTCC AGTGGTACCT GAGAGGAAGT CATCACTACC CCCAACATCA 
CCAATGGAAA AAATGTCCAA GTCACGGCTA TCAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL2 ( XM_006070034.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006070096.2 Bubalus bubalis NHS-like protein 2 (LOC102398431), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu73921
Clone ID Related Accession (Same CDS sequence)	XM_006070034.2
Accession Version	XM_006070034.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	NHS-like protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006070034.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC 
AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG 
CAAGAAGAAT ATGAGGAACA GTACTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT 
GCTGATGAGG CCCCTGAGCC CACCCCCAGC CCAAGGCTCC AGTCTGCCAG GCGTCTGGAG 
TTTGTACTGA TGCCCACAAA ATGGCAGCTG AGCGAGGATG AGACTACCAT GCAGGGTGTG 
AGGGCCCCTG AGGCCTCCCT GAGCCTGCCT ACCACAGCCG ACAAGCAAGC TGCCTGGAAT 
AGCCCCTTTC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC TTCAGTCTTG CCCCACACAC 
TCTGACATCG TGCCCATCAA CGTCTCCGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA 
CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG 
ACCATTATTG GATTCTCTAA CTATTCCCAG CGAGACCAAG GTCACAGCAA CAGCCCAGCA 
GGCAGTGTGG CCCACTCCAC CACCTCAGAC ATCAGGCCCA GCCATTCAGT TCCAGAAAGT 
GTTCATGGAA GAGTTGCACT TGGTCAGGAA GCTCGGTTCC CAAATCTCAC CTCACCAGTA 
CTAAGAAATC CTTCTAGTCA TCCGGAAGAA CCTCCCCAGG CATGGGGTGC CACAAAACCC 
CCTGGAATGG AGAGCATGGG GATGGTGTAC AGCATTCCTG GTTCTTGCAA TGGACCAACA 
GAATCGACAT TCTCTGCTTC CTGGAAGGGA GATGCTTTTA CCTATGTGAC TCCAAGTGCC 
ACCGCTCAGA GCAGTCAAGT CAATGAAAAT GGAAAAAATC CTTCCTCTGG GAATTCTTGG 
GTCTCTCTGC ACACACTGCC ACCTCTTGTT CCTAAGGAGG CTGCTACCCT CTTTGTCACT 
CGTGATATCC CAGCAGGGTG CAGTGGGCCT GCTGGCTACT CTCAGCATCC TACTCAACGA 
AGCCAAGTAT CTGAGCGACC CTCCAAGATT GGCCTTCTGA CCGGTGGTAC CTCAAGGCTG 
GAGACAGGCC CAGGTGGGGC CAGCAGGTTC CGGGAGCGGT CACTGTCTGT ACCCACAGAT 
ACAGGTACCA TGGATGTGGA CTATGAGGAG GAGCAGAAGG CCAGTGAAGC CTGTGCCCTA 
CCTTATGCCA GTACGAGTTC TGAGGGCAGT AACAGTGCTG ACAACATTGC CTCCCTCAGT 
GCCCAACAGG AGGCCCATCA CAGAAGGCAG AGGTCCAAGA GCATCTCACT CAAGAAGGCC 
AAGAAGAAGC CTTCCCCTCC GACTCGCAGT GTCTCACTGG TCAAAGATGA ACCAGTCCTC 
TTGCCGGAAG GTGGGTCAGT ACTACCCAAG GACCAGAGGC CCAGGAGCCT TTGTCTCTCC 
TTGGAACACC AAGGACATCA CTCATCCCAC CCAGATACTC AGGGTCACCC AGCTGTGCCA 
ACCTTCAAAG ACCCAGAAGG TACACAATTC GCCCATCACT GGTATCTTAC TGACTGGAAG 
TCTGGTGACA CCTACCAGTC CTTGTCCAGC TCCAGCACCG CCACTGGCAC CACAGTCATT 
GAGTGCACCC AAGTTCAGGG CAGCTCAGAG TCTCTTGCCT CCCCTTCCAC TTCCAGAGCC 
ACGACGCCTT CCCAGCTTTC CATCGAGGTG GAGGCCAGGG AGGTATCCTC TCCAGGAAGG 
CCTACTGGGC TGATGTCACC CTCCAGTGGA TACTCCAGCC AGTCAGAGAC GCCAACACCC 
ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC TTGCCCCCTC CAAGCAGCAG TGTCCGGGTG 
CGTCCAGTGG TACCTGAGAG GAAGTCATCA CTACCCCCAA CATCACCAAT GGAAAAAATG 
TCCAAGTCAC GGCTATCAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006070096.2
CDS	888..2951
Translation	MVAQPRAWNAAANSGRENATATAHSRSSWRQPVNVFLSSGRPPSVEELLREAQLNLQSLLQEEYEEQYSEARLLGQTFRSADEAPEPTPSPRLQSARRLEFVLMPTKWQLSEDETTMQGVRAPEASLSLPTTADKQAAWNSPFPLPILEEKRWLQSCPTHSDIVPINVSGQQFDKHASLRHSLFNTETAVNPKSTLRRRRTIIGFSNYSQRDQGHSNSPAGSVAHSTTSDIRPSHSVPESVHGRVALGQEARFPNLTSPVLRNPSSHPEEPPQAWGATKPPGMESMGMVYSIPGSCNGPTESTFSASWKGDAFTYVTPSATAQSSQVNENGKNPSSGNSWVSLHTLPPLVPKEAATLFVTRDIPAGCSGPAGYSQHPTQRSQVSERPSKIGLLTGGTSRLETGPGGASRFRERSLSVPTDTGTMDVDYEEEQKASEACALPYASTSSEGSNSADNIASLSAQQEAHHRRQRSKSISLKKAKKKPSPPTRSVSLVKDEPVLLPEGGSVLPKDQRPRSLCLSLEHQGHHSSHPDTQGHPAVPTFKDPEGTQFAHHWYLTDWKSGDTYQSLSSSSTATGTTVIECTQVQGSSESLASPSTSRATTPSQLSIEVEAREVSSPGRPTGLMSPSSGYSSQSETPTPTVSMSLTLGHLPPPSSSVRVRPVVPERKSSLPPTSPMEKMSKSRLSV

Target ORF information:

RefSeq Version	XM_006070034.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NHS-like protein 2 (LOC102398431), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006070034.2

ORF Insert Sequence:

ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC 
AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG 
CAAGAAGAAT ATGAGGAACA GTACTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT 
GCTGATGAGG CCCCTGAGCC CACCCCCAGC CCAAGGCTCC AGTCTGCCAG GCGTCTGGAG 
TTTGTACTGA TGCCCACAAA ATGGCAGCTG AGCGAGGATG AGACTACCAT GCAGGGTGTG 
AGGGCCCCTG AGGCCTCCCT GAGCCTGCCT ACCACAGCCG ACAAGCAAGC TGCCTGGAAT 
AGCCCCTTTC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC TTCAGTCTTG CCCCACACAC 
TCTGACATCG TGCCCATCAA CGTCTCCGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA 
CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG 
ACCATTATTG GATTCTCTAA CTATTCCCAG CGAGACCAAG GTCACAGCAA CAGCCCAGCA 
GGCAGTGTGG CCCACTCCAC CACCTCAGAC ATCAGGCCCA GCCATTCAGT TCCAGAAAGT 
GTTCATGGAA GAGTTGCACT TGGTCAGGAA GCTCGGTTCC CAAATCTCAC CTCACCAGTA 
CTAAGAAATC CTTCTAGTCA TCCGGAAGAA CCTCCCCAGG CATGGGGTGC CACAAAACCC 
CCTGGAATGG AGAGCATGGG GATGGTGTAC AGCATTCCTG GTTCTTGCAA TGGACCAACA 
GAATCGACAT TCTCTGCTTC CTGGAAGGGA GATGCTTTTA CCTATGTGAC TCCAAGTGCC 
ACCGCTCAGA GCAGTCAAGT CAATGAAAAT GGAAAAAATC CTTCCTCTGG GAATTCTTGG 
GTCTCTCTGC ACACACTGCC ACCTCTTGTT CCTAAGGAGG CTGCTACCCT CTTTGTCACT 
CGTGATATCC CAGCAGGGTG CAGTGGGCCT GCTGGCTACT CTCAGCATCC TACTCAACGA 
AGCCAAGTAT CTGAGCGACC CTCCAAGATT GGCCTTCTGA CCGGTGGTAC CTCAAGGCTG 
GAGACAGGCC CAGGTGGGGC CAGCAGGTTC CGGGAGCGGT CACTGTCTGT ACCCACAGAT 
ACAGGTACCA TGGATGTGGA CTATGAGGAG GAGCAGAAGG CCAGTGAAGC CTGTGCCCTA 
CCTTATGCCA GTACGAGTTC TGAGGGCAGT AACAGTGCTG ACAACATTGC CTCCCTCAGT 
GCCCAACAGG AGGCCCATCA CAGAAGGCAG AGGTCCAAGA GCATCTCACT CAAGAAGGCC 
AAGAAGAAGC CTTCCCCTCC GACTCGCAGT GTCTCACTGG TCAAAGATGA ACCAGTCCTC 
TTGCCGGAAG GTGGGTCAGT ACTACCCAAG GACCAGAGGC CCAGGAGCCT TTGTCTCTCC 
TTGGAACACC AAGGACATCA CTCATCCCAC CCAGATACTC AGGGTCACCC AGCTGTGCCA 
ACCTTCAAAG ACCCAGAAGG TACACAATTC GCCCATCACT GGTATCTTAC TGACTGGAAG 
TCTGGTGACA CCTACCAGTC CTTGTCCAGC TCCAGCACCG CCACTGGCAC CACAGTCATT 
GAGTGCACCC AAGTTCAGGG CAGCTCAGAG TCTCTTGCCT CCCCTTCCAC TTCCAGAGCC 
ACGACGCCTT CCCAGCTTTC CATCGAGGTG GAGGCCAGGG AGGTATCCTC TCCAGGAAGG 
CCTACTGGGC TGATGTCACC CTCCAGTGGA TACTCCAGCC AGTCAGAGAC GCCAACACCC 
ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC TTGCCCCCTC CAAGCAGCAG TGTCCGGGTG 
CGTCCAGTGG TACCTGAGAG GAAGTCATCA CTACCCCCAA CATCACCAAT GGAAAAAATG 
TCCAAGTCAC GGCTATCAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL2 ( XM_006070033.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006070095.1 Bubalus bubalis NHS-like 2 (NHSL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu24718
Clone ID Related Accession (Same CDS sequence)	XM_006070033.1
Accession Version	XM_006070033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	NHS-like protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785381.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CCGAGGCTAT CACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG 
TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAGCTGC TTCGCGAGGC GCAGCTCAAT 
CTCCAGAGCC TGTTGCAAGA AGAATATGAG GAACAGTACT CGGAGGCCAG ACTTCTGGGG 
CAGACCTTCC GCTCTGCTGA TGAGGCCCCT GAGCCCACCC CCAGCCCAAG GCTCCAGTCT 
GCCAGGCGTC TGGAGTTTGT ACTGATGCCC ACAAAATGGC AGCTGAGCGA GGATGAGACT 
ACCATGCAGG GTGTGAGGGC CCCTGAGGCC TCCCTGAGCC TGCCTACCAC AGCCGACAAG 
CAAGCTGCCT GGAATAGCCC CTTTCCTCTG CCCATCCTAG AGGAGAAGCG GTGGCTTCAG 
TCTTGCCCCA CACACTCTGA CATCGTGCCC ATCAACGTCT CCGGGCAGCA GTTTGATAAA 
CATGCAAGTT TGCGACACTC GTTGTTTAAC ACAGAGACAG CCGTGAACCC CAAGTCCACC 
CTGAGGCGGA GGCGGACCAT TATTGGATTC TCTAACTATT CCCAGCGAGA CCAAGGTCAC 
AGCAACAGCC CAGCAGGCAG TGTGGCCCAC TCCACCACCT CAGACATCAG GCCCAGCCAT 
TCAGTTCCAG AAAGTGTTCA TGGAAGAGTT GCACTTGGTC AGGAAGCTCG GTTCCCAAAT 
CTCACCTCAC CAGTACTAAG AAATCCTTCT AGTCATCCGG AAGAACCTCC CCAGGCATGG 
GGTGCCACAA AACCCCCTGG AATGGAGAGC ATGGGGATGG TGTACAGCAT TCCTGGTTCT 
TGCAATGGAC CAACAGAATC GACATTCTCT GCTTCCTGGA AGGGAGATGC TTTTACCTAT 
GTGACTCCAA GTGCCACCGC TCAGAGCAGT CAAGTCAATG AAAATGGAAA AAATCCTTCC 
TCTGGGAATT CTTGGGTCTC TCTGCACACA CTGCCACCTC TTGTTCCTAA GGAGGCTGCT 
ACCCTCTTTG TCACTCGTGA TATCCCAGCA GGGTGCAGTG GGCCTGCTGG CTACTCTCAG 
CATCCTACTC AACGAAGCCA AGTATCTGAG CGACCCTCCA AGATTGGCCT TCTGACCGGT 
GGTACCTCAA GGCTGGAGAC AGGCCCAGGT GGGGCCAGCA GGTTCCGGGA GCGGTCACTG 
TCTGTACCCA CAGATACAGG TACCATGGAT GTGGACTATG AGGAGGAGCA GAAGGCCAGT 
GAAGCCTGTG CCCTACCTTA TGCCAGTACG AGTTCTGAGG GCAGTAACAG TGCTGACAAC 
ATTGCCTCCC TCAGTGCCCA ACAGGAGGCC CATCACAGAA GGCAGAGGTC CAAGAGCATC 
TCACTCAAGA AGGCCAAGAA GAAGCCTTCC CCTCCGACTC GCAGTGTCTC ACTGGTCAAA 
GATGAACCAG TCCTCTTGCC GGAAGGTGGG TCAGTACTAC CCAAGGACCA GAGGCCCAGG 
AGCCTTTGTC TCTCCTTGGA ACACCAAGGA CATCACTCAT CCCACCCAGA TACTCAGGGT 
CACCCAGCTG TGCCAACCTT CAAAGACCCA GAAGGTACAC AATTCGCCCA TCACTGGTAT 
CTTACTGACT GGAAGTCTGG TGACACCTAC CAGTCCTTGT CCAGCTCCAG CACCGCCACT 
GGCACCACAG TCATTGAGTG CACCCAAGTT CAGGGCAGCT CAGAGTCTCT TGCCTCCCCT 
TCCACTTCCA GAGCCACGAC GCCTTCCCAG CTTTCCATCG AGGTGGAGGC CAGGGAGGTA 
TCCTCTCCAG GAAGGCCTAC TGGGCTGATG TCACCCTCCA GTGGATACTC CAGCCAGTCA 
GAGACGCCAA CACCCACTGT CTCCATGTCC TTGACCCTGG GCCACTTGCC CCCTCCAAGC 
AGCAGTGTCC GGGTGCGTCC AGTGGTACCT GAGAGGAAGT CATCACTACC CCCAACATCA 
CCAATGGAAA AAATGTCCAA GTCACGGCTA TCGTTTGACC TACCATTGAC CTCTTCAACC 
AACCTGGATC TGTCTGGGAT GAGTATCTCA ATCCGAAGCA AAAACAAGGT GAGCCAGCAT 
CACTCAGACA CAAATTTGGG GGCCAAGCTG GCCCAGAAAA CTAGCCCCAA CCAGCCAATC 
ATGCCTATGG TTACTCAGTC TGACTTACGT TCTGTTCGCC TGAGGTCAGT CAGCAAGTCT 
GAGCTGGAAG ATGACATTGA GAGCCCTGAC TATGCTGAGG AATCAGGAGC TGAAGTCTTC 
ACCTTGCCAG AGAGGAAGAT GAAACCTCCC ATAGCCGAGA AGCCCCCTCT GGCCCGAAGG 
CCTGCAAGCT TAGTCCACAA GCCACCATCT GTCCCCGAGG AGTACCCATT AACTTCGCCT 
ACCTTGGCTA TGACTCCCAA GAGCTCAATT CAGCACATAA GGCCACTCCC TCAAGATATC 
TACACAGTGG TGCGGAAATC AAAGTCCTCC AGCTTCCCTG AGAGCAGAAG CCCAGGGGAG 
TCCACAGCAC CCTCATCTCT TGTTTTCACG CCTTTTGCCA GTTCCTCTGG TGCTTTCTTC 
TCAGGAACAC AGCAACCTCC CCAGGGAAGT ATGGAGGATG AGGGCCCTAA GGGGAGAGCC 
CTGCCTGAAA GAATTAGCCT CCAGAGCCAA GAAGAAGCTG AGAAAAAGAA AGGCAAGATT 
CCACCTCCTG TACCAAAAAA GCCCAGCGTG CTGTACCTGC CTCTCACTTC ACCCACGGCT 
CAAGCGGAGG CCTATGTGAC TGAACCAAGA CTGCCCCTCA GCCCCATCAT CACCCTGGAG 
GAAGAGGCCA AGTGTCTCCC AACTGATGAT CACCTGCCAT CTGCTGGTAC AAGGATGACC 
TCAAGGCCAC AGTCCAACAG CGAAAGGGAA GCAAGTCCTC CAGGGAGTTT GATGGAACCA 
AGCACCGAAG AAAAAAGTGT AATCAGTGAT AAAACAGCCG AATGGATTGC AGAAGATGAT 
GATGATGTGT TTGTGGCTTC ACGTACAACT GAAGATTTAT TTACTGTGAT CCACAGGTCC 
AAAAGAAAGC TGCTTGGCTG GAAGGAACCT GGTGAGACCT TTGCTGGCAG CAGCAGACCA 
AGCTCCCACT CACCAGTAAG GAACCCACCT GAGTCTCCGG TCAGTGAGTT GGCTGCCTCT 
GCAGGGTCAA GCGGCAGTGC CAACCTAGAT GCTGGCAGAA ATGATGATTT CAAGGCCTTG 
CTACAGAAGA AGGGAAGCAA GGCAACTCCA AGGTCCCGCC CCTCAGCAGC CGAACTGCTG 
AAGACCACTA ACCCACTGGC TCGGCGAATT ATTGCACAAT TTTCAAAAGA CTACAAAACC 
CCTGATAACC CCAGTACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006070095.1
CDS	401..3841
Translation	MERAEAITLFWSRGAAANSGRENATATAHSRSSWRQPVNVFLSSGRPPSVEELLREAQLNLQSLLQEEYEEQYSEARLLGQTFRSADEAPEPTPSPRLQSARRLEFVLMPTKWQLSEDETTMQGVRAPEASLSLPTTADKQAAWNSPFPLPILEEKRWLQSCPTHSDIVPINVSGQQFDKHASLRHSLFNTETAVNPKSTLRRRRTIIGFSNYSQRDQGHSNSPAGSVAHSTTSDIRPSHSVPESVHGRVALGQEARFPNLTSPVLRNPSSHPEEPPQAWGATKPPGMESMGMVYSIPGSCNGPTESTFSASWKGDAFTYVTPSATAQSSQVNENGKNPSSGNSWVSLHTLPPLVPKEAATLFVTRDIPAGCSGPAGYSQHPTQRSQVSERPSKIGLLTGGTSRLETGPGGASRFRERSLSVPTDTGTMDVDYEEEQKASEACALPYASTSSEGSNSADNIASLSAQQEAHHRRQRSKSISLKKAKKKPSPPTRSVSLVKDEPVLLPEGGSVLPKDQRPRSLCLSLEHQGHHSSHPDTQGHPAVPTFKDPEGTQFAHHWYLTDWKSGDTYQSLSSSSTATGTTVIECTQVQGSSESLASPSTSRATTPSQLSIEVEAREVSSPGRPTGLMSPSSGYSSQSETPTPTVSMSLTLGHLPPPSSSVRVRPVVPERKSSLPPTSPMEKMSKSRLSFDLPLTSSTNLDLSGMSISIRSKNKVSQHHSDTNLGAKLAQKTSPNQPIMPMVTQSDLRSVRLRSVSKSELEDDIESPDYAEESGAEVFTLPERKMKPPIAEKPPLARRPASLVHKPPSVPEEYPLTSPTLAMTPKSSIQHIRPLPQDIYTVVRKSKSSSFPESRSPGESTAPSSLVFTPFASSSGAFFSGTQQPPQGSMEDEGPKGRALPERISLQSQEEAEKKKGKIPPPVPKKPSVLYLPLTSPTAQAEAYVTEPRLPLSPIITLEEEAKCLPTDDHLPSAGTRMTSRPQSNSEREASPPGSLMEPSTEEKSVISDKTAEWIAEDDDDVFVASRTTEDLFTVIHRSKRKLLGWKEPGETFAGSSRPSSHSPVRNPPESPVSELAASAGSSGSANLDAGRNDDFKALLQKKGSKATPRSRPSAAELLKTTNPLARRIIAQFSKDYKTPDNPST

Target ORF information:

RefSeq Version	XM_006070033.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NHS-like 2 (NHSL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006070033.1

ORF Insert Sequence:

ATGGAGAGAG CCGAGGCTAT CACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG 
TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAGCTGC TTCGCGAGGC GCAGCTCAAT 
CTCCAGAGCC TGTTGCAAGA AGAATATGAG GAACAGTACT CGGAGGCCAG ACTTCTGGGG 
CAGACCTTCC GCTCTGCTGA TGAGGCCCCT GAGCCCACCC CCAGCCCAAG GCTCCAGTCT 
GCCAGGCGTC TGGAGTTTGT ACTGATGCCC ACAAAATGGC AGCTGAGCGA GGATGAGACT 
ACCATGCAGG GTGTGAGGGC CCCTGAGGCC TCCCTGAGCC TGCCTACCAC AGCCGACAAG 
CAAGCTGCCT GGAATAGCCC CTTTCCTCTG CCCATCCTAG AGGAGAAGCG GTGGCTTCAG 
TCTTGCCCCA CACACTCTGA CATCGTGCCC ATCAACGTCT CCGGGCAGCA GTTTGATAAA 
CATGCAAGTT TGCGACACTC GTTGTTTAAC ACAGAGACAG CCGTGAACCC CAAGTCCACC 
CTGAGGCGGA GGCGGACCAT TATTGGATTC TCTAACTATT CCCAGCGAGA CCAAGGTCAC 
AGCAACAGCC CAGCAGGCAG TGTGGCCCAC TCCACCACCT CAGACATCAG GCCCAGCCAT 
TCAGTTCCAG AAAGTGTTCA TGGAAGAGTT GCACTTGGTC AGGAAGCTCG GTTCCCAAAT 
CTCACCTCAC CAGTACTAAG AAATCCTTCT AGTCATCCGG AAGAACCTCC CCAGGCATGG 
GGTGCCACAA AACCCCCTGG AATGGAGAGC ATGGGGATGG TGTACAGCAT TCCTGGTTCT 
TGCAATGGAC CAACAGAATC GACATTCTCT GCTTCCTGGA AGGGAGATGC TTTTACCTAT 
GTGACTCCAA GTGCCACCGC TCAGAGCAGT CAAGTCAATG AAAATGGAAA AAATCCTTCC 
TCTGGGAATT CTTGGGTCTC TCTGCACACA CTGCCACCTC TTGTTCCTAA GGAGGCTGCT 
ACCCTCTTTG TCACTCGTGA TATCCCAGCA GGGTGCAGTG GGCCTGCTGG CTACTCTCAG 
CATCCTACTC AACGAAGCCA AGTATCTGAG CGACCCTCCA AGATTGGCCT TCTGACCGGT 
GGTACCTCAA GGCTGGAGAC AGGCCCAGGT GGGGCCAGCA GGTTCCGGGA GCGGTCACTG 
TCTGTACCCA CAGATACAGG TACCATGGAT GTGGACTATG AGGAGGAGCA GAAGGCCAGT 
GAAGCCTGTG CCCTACCTTA TGCCAGTACG AGTTCTGAGG GCAGTAACAG TGCTGACAAC 
ATTGCCTCCC TCAGTGCCCA ACAGGAGGCC CATCACAGAA GGCAGAGGTC CAAGAGCATC 
TCACTCAAGA AGGCCAAGAA GAAGCCTTCC CCTCCGACTC GCAGTGTCTC ACTGGTCAAA 
GATGAACCAG TCCTCTTGCC GGAAGGTGGG TCAGTACTAC CCAAGGACCA GAGGCCCAGG 
AGCCTTTGTC TCTCCTTGGA ACACCAAGGA CATCACTCAT CCCACCCAGA TACTCAGGGT 
CACCCAGCTG TGCCAACCTT CAAAGACCCA GAAGGTACAC AATTCGCCCA TCACTGGTAT 
CTTACTGACT GGAAGTCTGG TGACACCTAC CAGTCCTTGT CCAGCTCCAG CACCGCCACT 
GGCACCACAG TCATTGAGTG CACCCAAGTT CAGGGCAGCT CAGAGTCTCT TGCCTCCCCT 
TCCACTTCCA GAGCCACGAC GCCTTCCCAG CTTTCCATCG AGGTGGAGGC CAGGGAGGTA 
TCCTCTCCAG GAAGGCCTAC TGGGCTGATG TCACCCTCCA GTGGATACTC CAGCCAGTCA 
GAGACGCCAA CACCCACTGT CTCCATGTCC TTGACCCTGG GCCACTTGCC CCCTCCAAGC 
AGCAGTGTCC GGGTGCGTCC AGTGGTACCT GAGAGGAAGT CATCACTACC CCCAACATCA 
CCAATGGAAA AAATGTCCAA GTCACGGCTA TCGTTTGACC TACCATTGAC CTCTTCAACC 
AACCTGGATC TGTCTGGGAT GAGTATCTCA ATCCGAAGCA AAAACAAGGT GAGCCAGCAT 
CACTCAGACA CAAATTTGGG GGCCAAGCTG GCCCAGAAAA CTAGCCCCAA CCAGCCAATC 
ATGCCTATGG TTACTCAGTC TGACTTACGT TCTGTTCGCC TGAGGTCAGT CAGCAAGTCT 
GAGCTGGAAG ATGACATTGA GAGCCCTGAC TATGCTGAGG AATCAGGAGC TGAAGTCTTC 
ACCTTGCCAG AGAGGAAGAT GAAACCTCCC ATAGCCGAGA AGCCCCCTCT GGCCCGAAGG 
CCTGCAAGCT TAGTCCACAA GCCACCATCT GTCCCCGAGG AGTACCCATT AACTTCGCCT 
ACCTTGGCTA TGACTCCCAA GAGCTCAATT CAGCACATAA GGCCACTCCC TCAAGATATC 
TACACAGTGG TGCGGAAATC AAAGTCCTCC AGCTTCCCTG AGAGCAGAAG CCCAGGGGAG 
TCCACAGCAC CCTCATCTCT TGTTTTCACG CCTTTTGCCA GTTCCTCTGG TGCTTTCTTC 
TCAGGAACAC AGCAACCTCC CCAGGGAAGT ATGGAGGATG AGGGCCCTAA GGGGAGAGCC 
CTGCCTGAAA GAATTAGCCT CCAGAGCCAA GAAGAAGCTG AGAAAAAGAA AGGCAAGATT 
CCACCTCCTG TACCAAAAAA GCCCAGCGTG CTGTACCTGC CTCTCACTTC ACCCACGGCT 
CAAGCGGAGG CCTATGTGAC TGAACCAAGA CTGCCCCTCA GCCCCATCAT CACCCTGGAG 
GAAGAGGCCA AGTGTCTCCC AACTGATGAT CACCTGCCAT CTGCTGGTAC AAGGATGACC 
TCAAGGCCAC AGTCCAACAG CGAAAGGGAA GCAAGTCCTC CAGGGAGTTT GATGGAACCA 
AGCACCGAAG AAAAAAGTGT AATCAGTGAT AAAACAGCCG AATGGATTGC AGAAGATGAT 
GATGATGTGT TTGTGGCTTC ACGTACAACT GAAGATTTAT TTACTGTGAT CCACAGGTCC 
AAAAGAAAGC TGCTTGGCTG GAAGGAACCT GGTGAGACCT TTGCTGGCAG CAGCAGACCA 
AGCTCCCACT CACCAGTAAG GAACCCACCT GAGTCTCCGG TCAGTGAGTT GGCTGCCTCT 
GCAGGGTCAA GCGGCAGTGC CAACCTAGAT GCTGGCAGAA ATGATGATTT CAAGGCCTTG 
CTACAGAAGA AGGGAAGCAA GGCAACTCCA AGGTCCCGCC CCTCAGCAGC CGAACTGCTG 
AAGACCACTA ACCCACTGGC TCGGCGAATT ATTGCACAAT TTTCAAAAGA CTACAAAACC 
CCTGATAACC CCAGTACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL2 ( XM_006070034.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006070096.1 Bubalus bubalis NHS-like 2 (NHSL2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu24719
Clone ID Related Accession (Same CDS sequence)	XM_006070034.1
Accession Version	XM_006070034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	NHS-like protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785381.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC 
AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG 
CAAGAAGAAT ATGAGGAACA GTACTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT 
GCTGATGAGG CCCCTGAGCC CACCCCCAGC CCAAGGCTCC AGTCTGCCAG GCGTCTGGAG 
TTTGTACTGA TGCCCACAAA ATGGCAGCTG AGCGAGGATG AGACTACCAT GCAGGGTGTG 
AGGGCCCCTG AGGCCTCCCT GAGCCTGCCT ACCACAGCCG ACAAGCAAGC TGCCTGGAAT 
AGCCCCTTTC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC TTCAGTCTTG CCCCACACAC 
TCTGACATCG TGCCCATCAA CGTCTCCGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA 
CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG 
ACCATTATTG GATTCTCTAA CTATTCCCAG CGAGACCAAG GTCACAGCAA CAGCCCAGCA 
GGCAGTGTGG CCCACTCCAC CACCTCAGAC ATCAGGCCCA GCCATTCAGT TCCAGAAAGT 
GTTCATGGAA GAGTTGCACT TGGTCAGGAA GCTCGGTTCC CAAATCTCAC CTCACCAGTA 
CTAAGAAATC CTTCTAGTCA TCCGGAAGAA CCTCCCCAGG CATGGGGTGC CACAAAACCC 
CCTGGAATGG AGAGCATGGG GATGGTGTAC AGCATTCCTG GTTCTTGCAA TGGACCAACA 
GAATCGACAT TCTCTGCTTC CTGGAAGGGA GATGCTTTTA CCTATGTGAC TCCAAGTGCC 
ACCGCTCAGA GCAGTCAAGT CAATGAAAAT GGAAAAAATC CTTCCTCTGG GAATTCTTGG 
GTCTCTCTGC ACACACTGCC ACCTCTTGTT CCTAAGGAGG CTGCTACCCT CTTTGTCACT 
CGTGATATCC CAGCAGGGTG CAGTGGGCCT GCTGGCTACT CTCAGCATCC TACTCAACGA 
AGCCAAGTAT CTGAGCGACC CTCCAAGATT GGCCTTCTGA CCGGTGGTAC CTCAAGGCTG 
GAGACAGGCC CAGGTGGGGC CAGCAGGTTC CGGGAGCGGT CACTGTCTGT ACCCACAGAT 
ACAGGTACCA TGGATGTGGA CTATGAGGAG GAGCAGAAGG CCAGTGAAGC CTGTGCCCTA 
CCTTATGCCA GTACGAGTTC TGAGGGCAGT AACAGTGCTG ACAACATTGC CTCCCTCAGT 
GCCCAACAGG AGGCCCATCA CAGAAGGCAG AGGTCCAAGA GCATCTCACT CAAGAAGGCC 
AAGAAGAAGC CTTCCCCTCC GACTCGCAGT GTCTCACTGG TCAAAGATGA ACCAGTCCTC 
TTGCCGGAAG GTGGGTCAGT ACTACCCAAG GACCAGAGGC CCAGGAGCCT TTGTCTCTCC 
TTGGAACACC AAGGACATCA CTCATCCCAC CCAGATACTC AGGGTCACCC AGCTGTGCCA 
ACCTTCAAAG ACCCAGAAGG TACACAATTC GCCCATCACT GGTATCTTAC TGACTGGAAG 
TCTGGTGACA CCTACCAGTC CTTGTCCAGC TCCAGCACCG CCACTGGCAC CACAGTCATT 
GAGTGCACCC AAGTTCAGGG CAGCTCAGAG TCTCTTGCCT CCCCTTCCAC TTCCAGAGCC 
ACGACGCCTT CCCAGCTTTC CATCGAGGTG GAGGCCAGGG AGGTATCCTC TCCAGGAAGG 
CCTACTGGGC TGATGTCACC CTCCAGTGGA TACTCCAGCC AGTCAGAGAC GCCAACACCC 
ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC TTGCCCCCTC CAAGCAGCAG TGTCCGGGTG 
CGTCCAGTGG TACCTGAGAG GAAGTCATCA CTACCCCCAA CATCACCAAT GGAAAAAATG 
TCCAAGTCAC GGCTATCGTT TGACCTACCA TTGACCTCTT CAACCAACCT GGATCTGTCT 
GGGATGAGTA TCTCAATCCG AAGCAAAAAC AAGGTGAGCC AGCATCACTC AGACACAAAT 
TTGGGGGCCA AGCTGGCCCA GAAAACTAGC CCCAACCAGC CAATCATGCC TATGGTTACT 
CAGTCTGACT TACGTTCTGT TCGCCTGAGG TCAGTCAGCA AGTCTGAGCT GGAAGATGAC 
ATTGAGAGCC CTGACTATGC TGAGGAATCA GGAGCTGAAG TCTTCACCTT GCCAGAGAGG 
AAGATGAAAC CTCCCATAGC CGAGAAGCCC CCTCTGGCCC GAAGGCCTGC AAGCTTAGTC 
CACAAGCCAC CATCTGTCCC CGAGGAGTAC CCATTAACTT CGCCTACCTT GGCTATGACT 
CCCAAGAGCT CAATTCAGCA CATAAGGCCA CTCCCTCAAG ATATCTACAC AGTGGTGCGG 
AAATCAAAGT CCTCCAGCTT CCCTGAGAGC AGAAGCCCAG GGGAGTCCAC AGCACCCTCA 
TCTCTTGTTT TCACGCCTTT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA 
CCTCCCCAGG GAAGTATGGA GGATGAGGGC CCTAAGGGGA GAGCCCTGCC TGAAAGAATT 
AGCCTCCAGA GCCAAGAAGA AGCTGAGAAA AAGAAAGGCA AGATTCCACC TCCTGTACCA 
AAAAAGCCCA GCGTGCTGTA CCTGCCTCTC ACTTCACCCA CGGCTCAAGC GGAGGCCTAT 
GTGACTGAAC CAAGACTGCC CCTCAGCCCC ATCATCACCC TGGAGGAAGA GGCCAAGTGT 
CTCCCAACTG ATGATCACCT GCCATCTGCT GGTACAAGGA TGACCTCAAG GCCACAGTCC 
AACAGCGAAA GGGAAGCAAG TCCTCCAGGG AGTTTGATGG AACCAAGCAC CGAAGAAAAA 
AGTGTAATCA GTGATAAAAC AGCCGAATGG ATTGCAGAAG ATGATGATGA TGTGTTTGTG 
GCTTCACGTA CAACTGAAGA TTTATTTACT GTGATCCACA GGTCCAAAAG AAAGCTGCTT 
GGCTGGAAGG AACCTGGTGA GACCTTTGCT GGCAGCAGCA GACCAAGCTC CCACTCACCA 
GTAAGGAACC CACCTGAGTC TCCGGTCAGT GAGTTGGCTG CCTCTGCAGG GTCAAGCGGC 
AGTGCCAACC TAGATGCTGG CAGAAATGAT GATTTCAAGG CCTTGCTACA GAAGAAGGGA 
AGCAAGGCAA CTCCAAGGTC CCGCCCCTCA GCAGCCGAAC TGCTGAAGAC CACTAACCCA 
CTGGCTCGGC GAATTATTGC ACAATTTTCA AAAGACTACA AAACCCCTGA TAACCCCAGT 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006070096.1
CDS	45..3470
Translation	MVAQPRAWNAAANSGRENATATAHSRSSWRQPVNVFLSSGRPPSVEELLREAQLNLQSLLQEEYEEQYSEARLLGQTFRSADEAPEPTPSPRLQSARRLEFVLMPTKWQLSEDETTMQGVRAPEASLSLPTTADKQAAWNSPFPLPILEEKRWLQSCPTHSDIVPINVSGQQFDKHASLRHSLFNTETAVNPKSTLRRRRTIIGFSNYSQRDQGHSNSPAGSVAHSTTSDIRPSHSVPESVHGRVALGQEARFPNLTSPVLRNPSSHPEEPPQAWGATKPPGMESMGMVYSIPGSCNGPTESTFSASWKGDAFTYVTPSATAQSSQVNENGKNPSSGNSWVSLHTLPPLVPKEAATLFVTRDIPAGCSGPAGYSQHPTQRSQVSERPSKIGLLTGGTSRLETGPGGASRFRERSLSVPTDTGTMDVDYEEEQKASEACALPYASTSSEGSNSADNIASLSAQQEAHHRRQRSKSISLKKAKKKPSPPTRSVSLVKDEPVLLPEGGSVLPKDQRPRSLCLSLEHQGHHSSHPDTQGHPAVPTFKDPEGTQFAHHWYLTDWKSGDTYQSLSSSSTATGTTVIECTQVQGSSESLASPSTSRATTPSQLSIEVEAREVSSPGRPTGLMSPSSGYSSQSETPTPTVSMSLTLGHLPPPSSSVRVRPVVPERKSSLPPTSPMEKMSKSRLSFDLPLTSSTNLDLSGMSISIRSKNKVSQHHSDTNLGAKLAQKTSPNQPIMPMVTQSDLRSVRLRSVSKSELEDDIESPDYAEESGAEVFTLPERKMKPPIAEKPPLARRPASLVHKPPSVPEEYPLTSPTLAMTPKSSIQHIRPLPQDIYTVVRKSKSSSFPESRSPGESTAPSSLVFTPFASSSGAFFSGTQQPPQGSMEDEGPKGRALPERISLQSQEEAEKKKGKIPPPVPKKPSVLYLPLTSPTAQAEAYVTEPRLPLSPIITLEEEAKCLPTDDHLPSAGTRMTSRPQSNSEREASPPGSLMEPSTEEKSVISDKTAEWIAEDDDDVFVASRTTEDLFTVIHRSKRKLLGWKEPGETFAGSSRPSSHSPVRNPPESPVSELAASAGSSGSANLDAGRNDDFKALLQKKGSKATPRSRPSAAELLKTTNPLARRIIAQFSKDYKTPDNPST

Target ORF information:

RefSeq Version	XM_006070034.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NHS-like 2 (NHSL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006070034.1

ORF Insert Sequence:

ATGGTGGCCC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC 
AGGCCCCCGA GTGTAGAGGA GCTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG 
CAAGAAGAAT ATGAGGAACA GTACTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT 
GCTGATGAGG CCCCTGAGCC CACCCCCAGC CCAAGGCTCC AGTCTGCCAG GCGTCTGGAG 
TTTGTACTGA TGCCCACAAA ATGGCAGCTG AGCGAGGATG AGACTACCAT GCAGGGTGTG 
AGGGCCCCTG AGGCCTCCCT GAGCCTGCCT ACCACAGCCG ACAAGCAAGC TGCCTGGAAT 
AGCCCCTTTC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC TTCAGTCTTG CCCCACACAC 
TCTGACATCG TGCCCATCAA CGTCTCCGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA 
CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG 
ACCATTATTG GATTCTCTAA CTATTCCCAG CGAGACCAAG GTCACAGCAA CAGCCCAGCA 
GGCAGTGTGG CCCACTCCAC CACCTCAGAC ATCAGGCCCA GCCATTCAGT TCCAGAAAGT 
GTTCATGGAA GAGTTGCACT TGGTCAGGAA GCTCGGTTCC CAAATCTCAC CTCACCAGTA 
CTAAGAAATC CTTCTAGTCA TCCGGAAGAA CCTCCCCAGG CATGGGGTGC CACAAAACCC 
CCTGGAATGG AGAGCATGGG GATGGTGTAC AGCATTCCTG GTTCTTGCAA TGGACCAACA 
GAATCGACAT TCTCTGCTTC CTGGAAGGGA GATGCTTTTA CCTATGTGAC TCCAAGTGCC 
ACCGCTCAGA GCAGTCAAGT CAATGAAAAT GGAAAAAATC CTTCCTCTGG GAATTCTTGG 
GTCTCTCTGC ACACACTGCC ACCTCTTGTT CCTAAGGAGG CTGCTACCCT CTTTGTCACT 
CGTGATATCC CAGCAGGGTG CAGTGGGCCT GCTGGCTACT CTCAGCATCC TACTCAACGA 
AGCCAAGTAT CTGAGCGACC CTCCAAGATT GGCCTTCTGA CCGGTGGTAC CTCAAGGCTG 
GAGACAGGCC CAGGTGGGGC CAGCAGGTTC CGGGAGCGGT CACTGTCTGT ACCCACAGAT 
ACAGGTACCA TGGATGTGGA CTATGAGGAG GAGCAGAAGG CCAGTGAAGC CTGTGCCCTA 
CCTTATGCCA GTACGAGTTC TGAGGGCAGT AACAGTGCTG ACAACATTGC CTCCCTCAGT 
GCCCAACAGG AGGCCCATCA CAGAAGGCAG AGGTCCAAGA GCATCTCACT CAAGAAGGCC 
AAGAAGAAGC CTTCCCCTCC GACTCGCAGT GTCTCACTGG TCAAAGATGA ACCAGTCCTC 
TTGCCGGAAG GTGGGTCAGT ACTACCCAAG GACCAGAGGC CCAGGAGCCT TTGTCTCTCC 
TTGGAACACC AAGGACATCA CTCATCCCAC CCAGATACTC AGGGTCACCC AGCTGTGCCA 
ACCTTCAAAG ACCCAGAAGG TACACAATTC GCCCATCACT GGTATCTTAC TGACTGGAAG 
TCTGGTGACA CCTACCAGTC CTTGTCCAGC TCCAGCACCG CCACTGGCAC CACAGTCATT 
GAGTGCACCC AAGTTCAGGG CAGCTCAGAG TCTCTTGCCT CCCCTTCCAC TTCCAGAGCC 
ACGACGCCTT CCCAGCTTTC CATCGAGGTG GAGGCCAGGG AGGTATCCTC TCCAGGAAGG 
CCTACTGGGC TGATGTCACC CTCCAGTGGA TACTCCAGCC AGTCAGAGAC GCCAACACCC 
ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC TTGCCCCCTC CAAGCAGCAG TGTCCGGGTG 
CGTCCAGTGG TACCTGAGAG GAAGTCATCA CTACCCCCAA CATCACCAAT GGAAAAAATG 
TCCAAGTCAC GGCTATCGTT TGACCTACCA TTGACCTCTT CAACCAACCT GGATCTGTCT 
GGGATGAGTA TCTCAATCCG AAGCAAAAAC AAGGTGAGCC AGCATCACTC AGACACAAAT 
TTGGGGGCCA AGCTGGCCCA GAAAACTAGC CCCAACCAGC CAATCATGCC TATGGTTACT 
CAGTCTGACT TACGTTCTGT TCGCCTGAGG TCAGTCAGCA AGTCTGAGCT GGAAGATGAC 
ATTGAGAGCC CTGACTATGC TGAGGAATCA GGAGCTGAAG TCTTCACCTT GCCAGAGAGG 
AAGATGAAAC CTCCCATAGC CGAGAAGCCC CCTCTGGCCC GAAGGCCTGC AAGCTTAGTC 
CACAAGCCAC CATCTGTCCC CGAGGAGTAC CCATTAACTT CGCCTACCTT GGCTATGACT 
CCCAAGAGCT CAATTCAGCA CATAAGGCCA CTCCCTCAAG ATATCTACAC AGTGGTGCGG 
AAATCAAAGT CCTCCAGCTT CCCTGAGAGC AGAAGCCCAG GGGAGTCCAC AGCACCCTCA 
TCTCTTGTTT TCACGCCTTT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA 
CCTCCCCAGG GAAGTATGGA GGATGAGGGC CCTAAGGGGA GAGCCCTGCC TGAAAGAATT 
AGCCTCCAGA GCCAAGAAGA AGCTGAGAAA AAGAAAGGCA AGATTCCACC TCCTGTACCA 
AAAAAGCCCA GCGTGCTGTA CCTGCCTCTC ACTTCACCCA CGGCTCAAGC GGAGGCCTAT 
GTGACTGAAC CAAGACTGCC CCTCAGCCCC ATCATCACCC TGGAGGAAGA GGCCAAGTGT 
CTCCCAACTG ATGATCACCT GCCATCTGCT GGTACAAGGA TGACCTCAAG GCCACAGTCC 
AACAGCGAAA GGGAAGCAAG TCCTCCAGGG AGTTTGATGG AACCAAGCAC CGAAGAAAAA 
AGTGTAATCA GTGATAAAAC AGCCGAATGG ATTGCAGAAG ATGATGATGA TGTGTTTGTG 
GCTTCACGTA CAACTGAAGA TTTATTTACT GTGATCCACA GGTCCAAAAG AAAGCTGCTT 
GGCTGGAAGG AACCTGGTGA GACCTTTGCT GGCAGCAGCA GACCAAGCTC CCACTCACCA 
GTAAGGAACC CACCTGAGTC TCCGGTCAGT GAGTTGGCTG CCTCTGCAGG GTCAAGCGGC 
AGTGCCAACC TAGATGCTGG CAGAAATGAT GATTTCAAGG CCTTGCTACA GAAGAAGGGA 
AGCAAGGCAA CTCCAAGGTC CCGCCCCTCA GCAGCCGAAC TGCTGAAGAC CACTAACCCA 
CTGGCTCGGC GAATTATTGC ACAATTTTCA AAAGACTACA AAACCCCTGA TAACCCCAGT 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NHSL2 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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