AKAP11 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	AKAP11
Entrez Gene ID	102393580
Full Name	A-kinase anchoring protein 11
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006076503.2	XP_006076565.1	A-kinase anchor protein 11 isoform X2
XM_006076502.2	XP_006076564.1	A-kinase anchor protein 11 isoform X1
XM_025263033.1	XP_025118818.1	A-kinase anchor protein 11 isoform X1
XM_025263035.1	XP_025118820.1	A-kinase anchor protein 11 isoform X3
XM_025263036.1	XP_025118821.1	A-kinase anchor protein 11 isoform X4
XM_006076503.1	XP_006076565.1	A-kinase anchor protein 11 isoform X2
XM_025263034.1	XP_025118819.1	A-kinase anchor protein 11 isoform X1
XM_006076502.1	XP_006076564.1	A-kinase anchor protein 11 isoform X1

Pathways from BioSystems
Gene Ontology

The following AKAP11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AKAP11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu30010	XM_006076502.2 Latest version!	Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu30010	XM_025263033.1 Latest version!	Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu30010	XM_025263034.1 Latest version!	Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu30011	XM_006076503.2 Latest version!	Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu47662	XM_025263035.1 Latest version!	Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu47663	XM_025263036.1 Latest version!	Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OBu30011	XM_006076503.1	Bubalus bubalis A kinase (PRKA) anchor protein 11 (AKAP11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu30010	XM_006076502.1	Bubalus bubalis A kinase (PRKA) anchor protein 11 (AKAP11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AKAP11 ( XM_006076502.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006076564.1 Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu30010
Clone ID Related Accession (Same CDS sequence)	XM_006076502.2 , XM_025263033.1 , XM_025263034.1 , XM_006076502.1
Accession Version	XM_006076502.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	A-kinase anchor protein 11 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006076502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCT TCCAGTCTTT CAGAAACAAT CACATGAAAA CTAGAGCATC TGTCAGAAAA 
AGCTTCAATG AAGATGTGTT CCAGTCTGTG AAGTCTTTAT TGCAGAGCGA GAAGGAACTA 
TGCAGTGTAT CAGCCGAGGA CTGTTTGAAG CAGGATGAAC ATGCCAATTT GACTGAGGTC 
ACATTTCTAG GTTTTAATGA AGAAACAGAT GCTGCTCATA TACAGGATTT GGCTGCAGTT 
TCTTTGGAAC TCCCAGATCT TCTGAACTCA CTGCACTTCT GCAGTCTAAA TGAAAATGAA 
ATTATTTGTA TGAAGGATAT AAATAAATCA TCAGATATAA ACAGTGATCC TCTAAACCAG 
AGTCAACATC CTGGAATGCT TTGTGTCATG AGAGTGTCAC CTACATTACC AAGACTTAAA 
ATTGATTTTA TTTTTAGTCT CCTAAGTAAA TATGCTACCG GCATAAGGTA CACCCTGGAC 
ACATATTTGC ATCACAAGCA CCAACTTGAG GCCACCAATG AAGACGACGA TGATACTAAC 
CAGTCTGTAT CTTCCATCGA AGATGACTTT GTCACTGCTT TTGAGCACTT AGACGAGGAA 
GAGACTTCAA AGCCATATAA TGATGGATTA AACATAATTG CACTAAGGAG CCAGTGTGAT 
GCTGCTTCAC AGACTATTTC TGGTCACCAT TTGGATACCC ATGATTTCAG GGTTCTGGTT 
GGCTCTGAGC AGCAGAAGTC ATTGGCTAAA CCTTCAGCTT CTTTAATAAA CATTCTGGGA 
CATAAAAAAC TGCCTCCAGC GAGAACTTCA GTCACGACAT CAGTCTCTGA ACCATGGATC 
CAGAGGAGTT TCTATAGTTC CTTTGCTGCT TCAGAGAAAG GTAGTGATGC CCAGAAACCA 
TTGTTCTCGT CTTCTCCTGC CTACTCGTCT GAATCAGAGT GCTCAAGTCC AAGTCCTGTT 
ATTTTCTTGG ATGAAGAAGG TTATCAAAAA AGTTTGAAAG CAAAACTTGA GTTACCTAAA 
ATTCCTGTGA TGAAAGATGA TATTGAGGAT TCAGACTCAG AAGTGAGTGA GTTTTTTGAT 
AGTTTTGATC AGTTTGATGA ACTAGAACAA ACGTTAGAGA CTGCTTGTCC GTTTCTGAAA 
GATCTTGCTG TAGGGAAGCC ATCGCAAAAG AAAGGGCACA AACATGAAAA ATTGTGCTCT 
GTAACCACTA CTATGAATCC TCAAAAATTC AAGTTTGATC GGCCAGCTCT CCCAGCTAAT 
GTTAGGAAGC CAACACCTCG TAAACCAGAA TCCCCTTATA GTAACCTGTG TGATGCCCCA 
GATTCTCCCC GCCCCGTGAA GGCCTCAGGG GAAGACAGTG GTTTGTTTAG TCCTATTCGA 
TCCTCTGCTT TCAGTCCTCT TGGAGGCTGT ACTCCAGCTG AATGCTTTTG CCAAACAGAT 
ATTGGTGGAG ATAGGGGTCA TGAAAATCCT GATTCTCTTT ATTATACCTA TGAAGATTAT 
GCAAATAGCC TTTCCTGTGA AGTTCTGGGC TCAGTTCTTC ATAGCCAACA TACTAATGCA 
ACATCCAACA CTAATAGTGT TAAAAAGGGA GAAAATAAAA TGGTAGCTTT TCAGCATGGA 
AGCCTTGATC AAAAAAGTAA ATCTAAAAAT AAACCCATAA TGATTGATAG CGTTCAGAAG 
TTTGCAGCAG ATCTCGTGGA AAAAAGTTTT GGCAGTGCAT TTAAAGACTT ACAGAAAGGG 
GTCTCTTCAT GTACCAATGC CTTATGCCAG TTAGCCATCA AATTGACGTC ATCCATTTTT 
CAAATGGCAT TTAACGAACT GAGAAGGCAG TGTGCCTTTT CACTGAAAGA ACGAGCCATT 
AGTGGCCTGG CTAGTTTTTT GGTGAGTGAG GCATTCTCAA ATGCTTTAAA AGATCTACAA 
TATGTAAAGA AACAGATCTT CACTAACACC GTCACTAGAT TTGCTGCAGA TCTTGCTGAA 
GAGCTTGTTT TTGAAGGCAT CATGGAAGTG TGTCAGTTCT CATATCCTCC AACACCTGCA 
TCTCTGCAGG GTCGGTCATT TCACTGTGAA GACAGAGTGG TGAAGTCCTA TGCAAGAGAT 
CTGTCTGAAT CTGTAATACA GGAAGCATTC ATTGAGCTGT CTCAAGTTGA TGTTACCTTT 
ACCACAAAGG CAGCAGTTAG TGTTTCCCCA GAAAACATCA AGTTTGTGAG CACAGAAGAT 
GTAGTGCCAG CAACACAGAC TTCTTTGTGT TCTTCTACTT CTAACAGTCA AACAGTTACG 
GTGACAAAAC CAGTGCAGGA GTATAAAAAG GAATACACGG TGCAGCAGGC CTTGTTCTGT 
ACTTCAGGAA TTGTTACTTC AATACCAGTG CCCTTGGCAG GAAGTGCCCT TCACCCATAC 
CGTATTTCTT CTAATACGTA TCAGACAAAG TCTCATCCAT CATGTGATGA TAGTCATTTG 
AATGGTGGTC CTACCCCAGC AAGTGTTGCC ACAAAAAACA AAGAAGAGGA GCTAGCTTGT 
CTCAGGAATA TTTGTTTACC TTCAGACCAC AACCCCGGTA GCCAGAATGA GGTGAAACCA 
ACTAGTGATG TTGTTGAAAC AAAAAGCTCT TCAAAGTTAA CAAATGATCC TGTCATTATT 
GGCGATTTTT CTGCCGCAAT GGTGCATACG ATAGTAAATG AAACTATAGA TTCACTGACA 
TCTTCCAAAG TTGCAAAAAC AGAAGAACAC ACAGATTGTT TAACTCAAAC AGGAAAGGGA 
AAAACCCCTT CTTTCTACTG GGATCAAGCA ACAGTGCCAC ACAGTGAGGC TAGAAAGAAG 
GACATGTTCG CTGATCGGTT ATCTAAATCT ATTATCAAAC ATTCCATAGA TGAAAGCAAG 
TCAGTGATCC TAAATGTAGA TAAAAATGTC CTCCCCAAGA AAGACTTGCC TGTTTCTGGA 
GATGAGTCAC AGTTGACCTT GGAAGAGTTC CCCAAGTTTC TTGAAGCTCA AGATCATTTA 
ACTCACTGTT CACTTTCAGA AGTAAAGGAT TGTGTTCCAG AAGGTAAAGG TTCTGTGGCT 
CCTGGATTTT CTTTGGAGAC GCTACCACCT TGTCTAGCAG CGGCAGGCCA GAAAGCTGAT 
CTGAAGGAAA TTGCTAAGGA CAAATCTGTG AAAAAGCAGA ATTTGCATAA TTCAGCCCTC 
GAGCCCTTGG CTTTTGGGCA GGAAAACTCT TTTCCCCATT CACATGCTTT CTCATCCACA 
GTGCTCACCT GTGTAGATGG TTTGCATGTG GAAGATAAAC AGAAAATGAG AGATGGGAAT 
GTAATACCTG ACACCCCTCC ATCAACTCCT CTGGTTCCAT CCCAGGCTCC TTCTGATTGG 
GATATCAAGA AGTTAACCAA AAAGCTTAAG GGGGAGTTAG CAAAAGAGTT TGCACCGGCC 
ACACCACCAT CCACACCTCA CAACTCATCT GTAAGCAGTT TGTCTGAAAA TGAACAAAAT 
GCAATAGAAA AAGAAGAGTT CATGTTGAAG CTCATGCGGT CTCTCTCAGA AGAAGTTGAA 
AGTAGTGACG GCGAAGAGCA GCAAGAGATG GACGTGAAAT TGGAGCCAGC AGGGAAAAAA 
GTTCAGTTTG CAGACGCATT AGCGACGCAC ATCGTTTCTC TTGCAACCGA AATGGCAGCT 
TCCCATTTGG ATAATAAATT GATTCAAGAA GCCGGGGTGA CAAGTCCTTG CTTAAATGTG 
CAGAGTCAAA GAAGCCTATC ACCTGCTTTT TTAAATTGCT CAGATGAAAG CTTACAAGCA 
TTATGCAGTT TTGCAGGTGA TATGGCAGCA GAAGTCATTA CAGAAGCTGA AAAAATAGCA 
AAAGTTAGGA GCTGTATGTT TTTCAGGAGG AAGAGGAACA GTTGTGTTGA TGGTGACCAA 
GATCGTAGAT CCGAAGAGAA GTTGGATGTA GAGGCTGTAG CGCACCCGGG AGGAGTAGAT 
CCGTTTGTTC TTCCTTTACC ACCAAGTTCT TGTCTGTCAG GTCTAACGTA CAAGTATCCC 
AGCTGTGAAA GTGTGACAGA TGAGTATGCA GGTCACATTA TCCAAATACT AAAACAGGAA 
GGTGGCAATA GTGAGTTAAT AATGGACCAG TACGCCAACA GACTTGCCTA TCGGTCTGTT 
AAGTCAGGAT TGCAAGAAGC AGCTAAAACA GCCAAAGTGA AGTGCAGCTC CAAAATACTC 
CCTGTGCAGA GCTCACAGGT GAAAACCAAC GATGAACTGT TACTGTTTTT AAATAAAGAA 
CACCAGCAAG AAGCAGATAA AAAAAGACAA AGCAGAAGTG CTGCAAGTTA CCTTCATAAA 
AACCGGACTT GTGAGTGGAC ACGGGACATG TACAGAAACG AGTGCTTGGA ACTACGTAGT 
TTTTCAACCT GTCTTGCTCA CAGCATCACA AGAGATGTTA AAAAAGAGTT GATGGCATCA 
ACAGGTGGCT TGCCAAAATC CTTAACAGAT TCTTGTCTTT ATGAAAAAAA CGTATGTGAC 
GAAGATACTG AGACTCATGT CGGGCCAGAG TTTCCCAAGT CTCTTCAGCC TTCTTCACAG 
AATCACAGAT TTTCTCAGAG TACAGGCAGC TTGAGTGGGT GTGGCTATGG AGAGACTGTC 
GTTCAAGCTA TAGAGCAGTA TGCTAACAAA GTCGTGGGCG ACACTTTAGA GCTGGGTTTA 
GGATCTGCCA TCTTCCACGT GTCTGAAGCC ACAAAAGCAG TTGATAGGAT TACTTACGCA 
GAAAAGCTGT CACCTCTGGT GGGTCAGGCT TGCCGATACT GTGACCGTAA AGAGCTCCAT 
GACTGCACTA GAAATTCGTC TCCTCACTTT CCCAGACCGG ATTCACTTGC TGGTAGTAAG 
CCAGTTTCTC ATTCACAGCT TAGCAGCTTC TATCAGAAAT CTAGGATTTT TCATCTTGAT 
GTTCCTCAAA TTCACGTTGA TCTCGATAAG AAGACAGTGC TTGCTGAGAA GATAGTTGCT 
GAAGCGATTG AAAAGGCAGA GAGAGAGCTG AGCAGTACCA GCCAGGCAGC CGACAGTGGG 
ATCGGACAGG AAGGTGTCAG CTTTGCCGAG AGCCTCAGCA CAGAGATACT GGCGTCAGCA 
GTGGTCAGTG CTGGACAGGC CATCAGCAGT CCAAAAGAAA CAGAAGAGAT TCAGTCAGCT 
GAGTCTCTTG GTAGCCAGCA GATGCATCTC AGTATTGGTG AGGACAGTAC TGGGAGCTGG 
TCCAACTTAA GTTTTGAAGA TGAACACCAG GATGAAAGCA GTAGTTTTCA CCATCTAAGT 
GAAAGTAATG GTAACAGCAG TAGCTGGAGC AGTCTTGGTT TAGAAGGAGA TTTGTATGAG 
GACAATTTAT CCTTTCCAAC ATCAGACAGT GATGGGCCAG ATGATGATGA AGAGGAGCGT 
CAGACTGATG TAGAAGGTTT GGAGCAAGAT GGAAAGACTC TGCTAATTAC AAATATTGAC 
ATGGAGCCAT GCACAGTAGA CCCCCAGCTA AGGATTATTC TTCAGTGGCT CGTTGCTTCT 
GAGGCAGAAG TTGCAGAACT TTATTTTCAT GACTCTACAA AGAAGGAGTT TATGCAACTT 
TCAAAACGGC TACAGGAAAA AGGGTGGAAG GTGGGAGACC TCCTGCAGGC TGTGCTTAAA 
TACTATGAGG AGGTGGAGAA GAAGTCCAGT GAGGAGAGAT GCAAGTCCCT GTTTGATTGG 
CTCTTGGAAA ATGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006076564.1
CDS	178..5895
Translation	MATFQSFRNNHMKTRASVRKSFNEDVFQSVKSLLQSEKELCSVSAEDCLKQDEHANLTEVTFLGFNEETDAAHIQDLAAVSLELPDLLNSLHFCSLNENEIICMKDINKSSDINSDPLNQSQHPGMLCVMRVSPTLPRLKIDFIFSLLSKYATGIRYTLDTYLHHKHQLEATNEDDDDTNQSVSSIEDDFVTAFEHLDEEETSKPYNDGLNIIALRSQCDAASQTISGHHLDTHDFRVLVGSEQQKSLAKPSASLINILGHKKLPPARTSVTTSVSEPWIQRSFYSSFAASEKGSDAQKPLFSSSPAYSSESECSSPSPVIFLDEEGYQKSLKAKLELPKIPVMKDDIEDSDSEVSEFFDSFDQFDELEQTLETACPFLKDLAVGKPSQKKGHKHEKLCSVTTTMNPQKFKFDRPALPANVRKPTPRKPESPYSNLCDAPDSPRPVKASGEDSGLFSPIRSSAFSPLGGCTPAECFCQTDIGGDRGHENPDSLYYTYEDYANSLSCEVLGSVLHSQHTNATSNTNSVKKGENKMVAFQHGSLDQKSKSKNKPIMIDSVQKFAADLVEKSFGSAFKDLQKGVSSCTNALCQLAIKLTSSIFQMAFNELRRQCAFSLKERAISGLASFLVSEAFSNALKDLQYVKKQIFTNTVTRFAADLAEELVFEGIMEVCQFSYPPTPASLQGRSFHCEDRVVKSYARDLSESVIQEAFIELSQVDVTFTTKAAVSVSPENIKFVSTEDVVPATQTSLCSSTSNSQTVTVTKPVQEYKKEYTVQQALFCTSGIVTSIPVPLAGSALHPYRISSNTYQTKSHPSCDDSHLNGGPTPASVATKNKEEELACLRNICLPSDHNPGSQNEVKPTSDVVETKSSSKLTNDPVIIGDFSAAMVHTIVNETIDSLTSSKVAKTEEHTDCLTQTGKGKTPSFYWDQATVPHSEARKKDMFADRLSKSIIKHSIDESKSVILNVDKNVLPKKDLPVSGDESQLTLEEFPKFLEAQDHLTHCSLSEVKDCVPEGKGSVAPGFSLETLPPCLAAAGQKADLKEIAKDKSVKKQNLHNSALEPLAFGQENSFPHSHAFSSTVLTCVDGLHVEDKQKMRDGNVIPDTPPSTPLVPSQAPSDWDIKKLTKKLKGELAKEFAPATPPSTPHNSSVSSLSENEQNAIEKEEFMLKLMRSLSEEVESSDGEEQQEMDVKLEPAGKKVQFADALATHIVSLATEMAASHLDNKLIQEAGVTSPCLNVQSQRSLSPAFLNCSDESLQALCSFAGDMAAEVITEAEKIAKVRSCMFFRRKRNSCVDGDQDRRSEEKLDVEAVAHPGGVDPFVLPLPPSSCLSGLTYKYPSCESVTDEYAGHIIQILKQEGGNSELIMDQYANRLAYRSVKSGLQEAAKTAKVKCSSKILPVQSSQVKTNDELLLFLNKEHQQEADKKRQSRSAASYLHKNRTCEWTRDMYRNECLELRSFSTCLAHSITRDVKKELMASTGGLPKSLTDSCLYEKNVCDEDTETHVGPEFPKSLQPSSQNHRFSQSTGSLSGCGYGETVVQAIEQYANKVVGDTLELGLGSAIFHVSEATKAVDRITYAEKLSPLVGQACRYCDRKELHDCTRNSSPHFPRPDSLAGSKPVSHSQLSSFYQKSRIFHLDVPQIHVDLDKKTVLAEKIVAEAIEKAERELSSTSQAADSGIGQEGVSFAESLSTEILASAVVSAGQAISSPKETEEIQSAESLGSQQMHLSIGEDSTGSWSNLSFEDEHQDESSSFHHLSESNGNSSSWSSLGLEGDLYEDNLSFPTSDSDGPDDDEEERQTDVEGLEQDGKTLLITNIDMEPCTVDPQLRIILQWLVASEAEVAELYFHDSTKKEFMQLSKRLQEKGWKVGDLLQAVLKYYEEVEKKSSEERCKSLFDWLLENA

Target ORF information:

RefSeq Version	XM_006076502.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006076502.2

ORF Insert Sequence:

ATGGCGACCT TCCAGTCTTT CAGAAACAAT CACATGAAAA CTAGAGCATC TGTCAGAAAA 
AGCTTCAATG AAGATGTGTT CCAGTCTGTG AAGTCTTTAT TGCAGAGCGA GAAGGAACTA 
TGCAGTGTAT CAGCCGAGGA CTGTTTGAAG CAGGATGAAC ATGCCAATTT GACTGAGGTC 
ACATTTCTAG GTTTTAATGA AGAAACAGAT GCTGCTCATA TACAGGATTT GGCTGCAGTT 
TCTTTGGAAC TCCCAGATCT TCTGAACTCA CTGCACTTCT GCAGTCTAAA TGAAAATGAA 
ATTATTTGTA TGAAGGATAT AAATAAATCA TCAGATATAA ACAGTGATCC TCTAAACCAG 
AGTCAACATC CTGGAATGCT TTGTGTCATG AGAGTGTCAC CTACATTACC AAGACTTAAA 
ATTGATTTTA TTTTTAGTCT CCTAAGTAAA TATGCTACCG GCATAAGGTA CACCCTGGAC 
ACATATTTGC ATCACAAGCA CCAACTTGAG GCCACCAATG AAGACGACGA TGATACTAAC 
CAGTCTGTAT CTTCCATCGA AGATGACTTT GTCACTGCTT TTGAGCACTT AGACGAGGAA 
GAGACTTCAA AGCCATATAA TGATGGATTA AACATAATTG CACTAAGGAG CCAGTGTGAT 
GCTGCTTCAC AGACTATTTC TGGTCACCAT TTGGATACCC ATGATTTCAG GGTTCTGGTT 
GGCTCTGAGC AGCAGAAGTC ATTGGCTAAA CCTTCAGCTT CTTTAATAAA CATTCTGGGA 
CATAAAAAAC TGCCTCCAGC GAGAACTTCA GTCACGACAT CAGTCTCTGA ACCATGGATC 
CAGAGGAGTT TCTATAGTTC CTTTGCTGCT TCAGAGAAAG GTAGTGATGC CCAGAAACCA 
TTGTTCTCGT CTTCTCCTGC CTACTCGTCT GAATCAGAGT GCTCAAGTCC AAGTCCTGTT 
ATTTTCTTGG ATGAAGAAGG TTATCAAAAA AGTTTGAAAG CAAAACTTGA GTTACCTAAA 
ATTCCTGTGA TGAAAGATGA TATTGAGGAT TCAGACTCAG AAGTGAGTGA GTTTTTTGAT 
AGTTTTGATC AGTTTGATGA ACTAGAACAA ACGTTAGAGA CTGCTTGTCC GTTTCTGAAA 
GATCTTGCTG TAGGGAAGCC ATCGCAAAAG AAAGGGCACA AACATGAAAA ATTGTGCTCT 
GTAACCACTA CTATGAATCC TCAAAAATTC AAGTTTGATC GGCCAGCTCT CCCAGCTAAT 
GTTAGGAAGC CAACACCTCG TAAACCAGAA TCCCCTTATA GTAACCTGTG TGATGCCCCA 
GATTCTCCCC GCCCCGTGAA GGCCTCAGGG GAAGACAGTG GTTTGTTTAG TCCTATTCGA 
TCCTCTGCTT TCAGTCCTCT TGGAGGCTGT ACTCCAGCTG AATGCTTTTG CCAAACAGAT 
ATTGGTGGAG ATAGGGGTCA TGAAAATCCT GATTCTCTTT ATTATACCTA TGAAGATTAT 
GCAAATAGCC TTTCCTGTGA AGTTCTGGGC TCAGTTCTTC ATAGCCAACA TACTAATGCA 
ACATCCAACA CTAATAGTGT TAAAAAGGGA GAAAATAAAA TGGTAGCTTT TCAGCATGGA 
AGCCTTGATC AAAAAAGTAA ATCTAAAAAT AAACCCATAA TGATTGATAG CGTTCAGAAG 
TTTGCAGCAG ATCTCGTGGA AAAAAGTTTT GGCAGTGCAT TTAAAGACTT ACAGAAAGGG 
GTCTCTTCAT GTACCAATGC CTTATGCCAG TTAGCCATCA AATTGACGTC ATCCATTTTT 
CAAATGGCAT TTAACGAACT GAGAAGGCAG TGTGCCTTTT CACTGAAAGA ACGAGCCATT 
AGTGGCCTGG CTAGTTTTTT GGTGAGTGAG GCATTCTCAA ATGCTTTAAA AGATCTACAA 
TATGTAAAGA AACAGATCTT CACTAACACC GTCACTAGAT TTGCTGCAGA TCTTGCTGAA 
GAGCTTGTTT TTGAAGGCAT CATGGAAGTG TGTCAGTTCT CATATCCTCC AACACCTGCA 
TCTCTGCAGG GTCGGTCATT TCACTGTGAA GACAGAGTGG TGAAGTCCTA TGCAAGAGAT 
CTGTCTGAAT CTGTAATACA GGAAGCATTC ATTGAGCTGT CTCAAGTTGA TGTTACCTTT 
ACCACAAAGG CAGCAGTTAG TGTTTCCCCA GAAAACATCA AGTTTGTGAG CACAGAAGAT 
GTAGTGCCAG CAACACAGAC TTCTTTGTGT TCTTCTACTT CTAACAGTCA AACAGTTACG 
GTGACAAAAC CAGTGCAGGA GTATAAAAAG GAATACACGG TGCAGCAGGC CTTGTTCTGT 
ACTTCAGGAA TTGTTACTTC AATACCAGTG CCCTTGGCAG GAAGTGCCCT TCACCCATAC 
CGTATTTCTT CTAATACGTA TCAGACAAAG TCTCATCCAT CATGTGATGA TAGTCATTTG 
AATGGTGGTC CTACCCCAGC AAGTGTTGCC ACAAAAAACA AAGAAGAGGA GCTAGCTTGT 
CTCAGGAATA TTTGTTTACC TTCAGACCAC AACCCCGGTA GCCAGAATGA GGTGAAACCA 
ACTAGTGATG TTGTTGAAAC AAAAAGCTCT TCAAAGTTAA CAAATGATCC TGTCATTATT 
GGCGATTTTT CTGCCGCAAT GGTGCATACG ATAGTAAATG AAACTATAGA TTCACTGACA 
TCTTCCAAAG TTGCAAAAAC AGAAGAACAC ACAGATTGTT TAACTCAAAC AGGAAAGGGA 
AAAACCCCTT CTTTCTACTG GGATCAAGCA ACAGTGCCAC ACAGTGAGGC TAGAAAGAAG 
GACATGTTCG CTGATCGGTT ATCTAAATCT ATTATCAAAC ATTCCATAGA TGAAAGCAAG 
TCAGTGATCC TAAATGTAGA TAAAAATGTC CTCCCCAAGA AAGACTTGCC TGTTTCTGGA 
GATGAGTCAC AGTTGACCTT GGAAGAGTTC CCCAAGTTTC TTGAAGCTCA AGATCATTTA 
ACTCACTGTT CACTTTCAGA AGTAAAGGAT TGTGTTCCAG AAGGTAAAGG TTCTGTGGCT 
CCTGGATTTT CTTTGGAGAC GCTACCACCT TGTCTAGCAG CGGCAGGCCA GAAAGCTGAT 
CTGAAGGAAA TTGCTAAGGA CAAATCTGTG AAAAAGCAGA ATTTGCATAA TTCAGCCCTC 
GAGCCCTTGG CTTTTGGGCA GGAAAACTCT TTTCCCCATT CACATGCTTT CTCATCCACA 
GTGCTCACCT GTGTAGATGG TTTGCATGTG GAAGATAAAC AGAAAATGAG AGATGGGAAT 
GTAATACCTG ACACCCCTCC ATCAACTCCT CTGGTTCCAT CCCAGGCTCC TTCTGATTGG 
GATATCAAGA AGTTAACCAA AAAGCTTAAG GGGGAGTTAG CAAAAGAGTT TGCACCGGCC 
ACACCACCAT CCACACCTCA CAACTCATCT GTAAGCAGTT TGTCTGAAAA TGAACAAAAT 
GCAATAGAAA AAGAAGAGTT CATGTTGAAG CTCATGCGGT CTCTCTCAGA AGAAGTTGAA 
AGTAGTGACG GCGAAGAGCA GCAAGAGATG GACGTGAAAT TGGAGCCAGC AGGGAAAAAA 
GTTCAGTTTG CAGACGCATT AGCGACGCAC ATCGTTTCTC TTGCAACCGA AATGGCAGCT 
TCCCATTTGG ATAATAAATT GATTCAAGAA GCCGGGGTGA CAAGTCCTTG CTTAAATGTG 
CAGAGTCAAA GAAGCCTATC ACCTGCTTTT TTAAATTGCT CAGATGAAAG CTTACAAGCA 
TTATGCAGTT TTGCAGGTGA TATGGCAGCA GAAGTCATTA CAGAAGCTGA AAAAATAGCA 
AAAGTTAGGA GCTGTATGTT TTTCAGGAGG AAGAGGAACA GTTGTGTTGA TGGTGACCAA 
GATCGTAGAT CCGAAGAGAA GTTGGATGTA GAGGCTGTAG CGCACCCGGG AGGAGTAGAT 
CCGTTTGTTC TTCCTTTACC ACCAAGTTCT TGTCTGTCAG GTCTAACGTA CAAGTATCCC 
AGCTGTGAAA GTGTGACAGA TGAGTATGCA GGTCACATTA TCCAAATACT AAAACAGGAA 
GGTGGCAATA GTGAGTTAAT AATGGACCAG TACGCCAACA GACTTGCCTA TCGGTCTGTT 
AAGTCAGGAT TGCAAGAAGC AGCTAAAACA GCCAAAGTGA AGTGCAGCTC CAAAATACTC 
CCTGTGCAGA GCTCACAGGT GAAAACCAAC GATGAACTGT TACTGTTTTT AAATAAAGAA 
CACCAGCAAG AAGCAGATAA AAAAAGACAA AGCAGAAGTG CTGCAAGTTA CCTTCATAAA 
AACCGGACTT GTGAGTGGAC ACGGGACATG TACAGAAACG AGTGCTTGGA ACTACGTAGT 
TTTTCAACCT GTCTTGCTCA CAGCATCACA AGAGATGTTA AAAAAGAGTT GATGGCATCA 
ACAGGTGGCT TGCCAAAATC CTTAACAGAT TCTTGTCTTT ATGAAAAAAA CGTATGTGAC 
GAAGATACTG AGACTCATGT CGGGCCAGAG TTTCCCAAGT CTCTTCAGCC TTCTTCACAG 
AATCACAGAT TTTCTCAGAG TACAGGCAGC TTGAGTGGGT GTGGCTATGG AGAGACTGTC 
GTTCAAGCTA TAGAGCAGTA TGCTAACAAA GTCGTGGGCG ACACTTTAGA GCTGGGTTTA 
GGATCTGCCA TCTTCCACGT GTCTGAAGCC ACAAAAGCAG TTGATAGGAT TACTTACGCA 
GAAAAGCTGT CACCTCTGGT GGGTCAGGCT TGCCGATACT GTGACCGTAA AGAGCTCCAT 
GACTGCACTA GAAATTCGTC TCCTCACTTT CCCAGACCGG ATTCACTTGC TGGTAGTAAG 
CCAGTTTCTC ATTCACAGCT TAGCAGCTTC TATCAGAAAT CTAGGATTTT TCATCTTGAT 
GTTCCTCAAA TTCACGTTGA TCTCGATAAG AAGACAGTGC TTGCTGAGAA GATAGTTGCT 
GAAGCGATTG AAAAGGCAGA GAGAGAGCTG AGCAGTACCA GCCAGGCAGC CGACAGTGGG 
ATCGGACAGG AAGGTGTCAG CTTTGCCGAG AGCCTCAGCA CAGAGATACT GGCGTCAGCA 
GTGGTCAGTG CTGGACAGGC CATCAGCAGT CCAAAAGAAA CAGAAGAGAT TCAGTCAGCT 
GAGTCTCTTG GTAGCCAGCA GATGCATCTC AGTATTGGTG AGGACAGTAC TGGGAGCTGG 
TCCAACTTAA GTTTTGAAGA TGAACACCAG GATGAAAGCA GTAGTTTTCA CCATCTAAGT 
GAAAGTAATG GTAACAGCAG TAGCTGGAGC AGTCTTGGTT TAGAAGGAGA TTTGTATGAG 
GACAATTTAT CCTTTCCAAC ATCAGACAGT GATGGGCCAG ATGATGATGA AGAGGAGCGT 
CAGACTGATG TAGAAGGTTT GGAGCAAGAT GGAAAGACTC TGCTAATTAC AAATATTGAC 
ATGGAGCCAT GCACAGTAGA CCCCCAGCTA AGGATTATTC TTCAGTGGCT CGTTGCTTCT 
GAGGCAGAAG TTGCAGAACT TTATTTTCAT GACTCTACAA AGAAGGAGTT TATGCAACTT 
TCAAAACGGC TACAGGAAAA AGGGTGGAAG GTGGGAGACC TCCTGCAGGC TGTGCTTAAA 
TACTATGAGG AGGTGGAGAA GAAGTCCAGT GAGGAGAGAT GCAAGTCCCT GTTTGATTGG 
CTCTTGGAAA ATGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP11 ( XM_025263033.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025118818.1 Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X2, mRNA.

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CloneID	OBu30010
Clone ID Related Accession (Same CDS sequence)	XM_006076502.2 , XM_025263033.1 , XM_025263034.1 , XM_006076502.1
Accession Version	XM_025263033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	A-kinase anchor protein 11 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCT TCCAGTCTTT CAGAAACAAT CACATGAAAA CTAGAGCATC TGTCAGAAAA 
AGCTTCAATG AAGATGTGTT CCAGTCTGTG AAGTCTTTAT TGCAGAGCGA GAAGGAACTA 
TGCAGTGTAT CAGCCGAGGA CTGTTTGAAG CAGGATGAAC ATGCCAATTT GACTGAGGTC 
ACATTTCTAG GTTTTAATGA AGAAACAGAT GCTGCTCATA TACAGGATTT GGCTGCAGTT 
TCTTTGGAAC TCCCAGATCT TCTGAACTCA CTGCACTTCT GCAGTCTAAA TGAAAATGAA 
ATTATTTGTA TGAAGGATAT AAATAAATCA TCAGATATAA ACAGTGATCC TCTAAACCAG 
AGTCAACATC CTGGAATGCT TTGTGTCATG AGAGTGTCAC CTACATTACC AAGACTTAAA 
ATTGATTTTA TTTTTAGTCT CCTAAGTAAA TATGCTACCG GCATAAGGTA CACCCTGGAC 
ACATATTTGC ATCACAAGCA CCAACTTGAG GCCACCAATG AAGACGACGA TGATACTAAC 
CAGTCTGTAT CTTCCATCGA AGATGACTTT GTCACTGCTT TTGAGCACTT AGACGAGGAA 
GAGACTTCAA AGCCATATAA TGATGGATTA AACATAATTG CACTAAGGAG CCAGTGTGAT 
GCTGCTTCAC AGACTATTTC TGGTCACCAT TTGGATACCC ATGATTTCAG GGTTCTGGTT 
GGCTCTGAGC AGCAGAAGTC ATTGGCTAAA CCTTCAGCTT CTTTAATAAA CATTCTGGGA 
CATAAAAAAC TGCCTCCAGC GAGAACTTCA GTCACGACAT CAGTCTCTGA ACCATGGATC 
CAGAGGAGTT TCTATAGTTC CTTTGCTGCT TCAGAGAAAG GTAGTGATGC CCAGAAACCA 
TTGTTCTCGT CTTCTCCTGC CTACTCGTCT GAATCAGAGT GCTCAAGTCC AAGTCCTGTT 
ATTTTCTTGG ATGAAGAAGG TTATCAAAAA AGTTTGAAAG CAAAACTTGA GTTACCTAAA 
ATTCCTGTGA TGAAAGATGA TATTGAGGAT TCAGACTCAG AAGTGAGTGA GTTTTTTGAT 
AGTTTTGATC AGTTTGATGA ACTAGAACAA ACGTTAGAGA CTGCTTGTCC GTTTCTGAAA 
GATCTTGCTG TAGGGAAGCC ATCGCAAAAG AAAGGGCACA AACATGAAAA ATTGTGCTCT 
GTAACCACTA CTATGAATCC TCAAAAATTC AAGTTTGATC GGCCAGCTCT CCCAGCTAAT 
GTTAGGAAGC CAACACCTCG TAAACCAGAA TCCCCTTATA GTAACCTGTG TGATGCCCCA 
GATTCTCCCC GCCCCGTGAA GGCCTCAGGG GAAGACAGTG GTTTGTTTAG TCCTATTCGA 
TCCTCTGCTT TCAGTCCTCT TGGAGGCTGT ACTCCAGCTG AATGCTTTTG CCAAACAGAT 
ATTGGTGGAG ATAGGGGTCA TGAAAATCCT GATTCTCTTT ATTATACCTA TGAAGATTAT 
GCAAATAGCC TTTCCTGTGA AGTTCTGGGC TCAGTTCTTC ATAGCCAACA TACTAATGCA 
ACATCCAACA CTAATAGTGT TAAAAAGGGA GAAAATAAAA TGGTAGCTTT TCAGCATGGA 
AGCCTTGATC AAAAAAGTAA ATCTAAAAAT AAACCCATAA TGATTGATAG CGTTCAGAAG 
TTTGCAGCAG ATCTCGTGGA AAAAAGTTTT GGCAGTGCAT TTAAAGACTT ACAGAAAGGG 
GTCTCTTCAT GTACCAATGC CTTATGCCAG TTAGCCATCA AATTGACGTC ATCCATTTTT 
CAAATGGCAT TTAACGAACT GAGAAGGCAG TGTGCCTTTT CACTGAAAGA ACGAGCCATT 
AGTGGCCTGG CTAGTTTTTT GGTGAGTGAG GCATTCTCAA ATGCTTTAAA AGATCTACAA 
TATGTAAAGA AACAGATCTT CACTAACACC GTCACTAGAT TTGCTGCAGA TCTTGCTGAA 
GAGCTTGTTT TTGAAGGCAT CATGGAAGTG TGTCAGTTCT CATATCCTCC AACACCTGCA 
TCTCTGCAGG GTCGGTCATT TCACTGTGAA GACAGAGTGG TGAAGTCCTA TGCAAGAGAT 
CTGTCTGAAT CTGTAATACA GGAAGCATTC ATTGAGCTGT CTCAAGTTGA TGTTACCTTT 
ACCACAAAGG CAGCAGTTAG TGTTTCCCCA GAAAACATCA AGTTTGTGAG CACAGAAGAT 
GTAGTGCCAG CAACACAGAC TTCTTTGTGT TCTTCTACTT CTAACAGTCA AACAGTTACG 
GTGACAAAAC CAGTGCAGGA GTATAAAAAG GAATACACGG TGCAGCAGGC CTTGTTCTGT 
ACTTCAGGAA TTGTTACTTC AATACCAGTG CCCTTGGCAG GAAGTGCCCT TCACCCATAC 
CGTATTTCTT CTAATACGTA TCAGACAAAG TCTCATCCAT CATGTGATGA TAGTCATTTG 
AATGGTGGTC CTACCCCAGC AAGTGTTGCC ACAAAAAACA AAGAAGAGGA GCTAGCTTGT 
CTCAGGAATA TTTGTTTACC TTCAGACCAC AACCCCGGTA GCCAGAATGA GGTGAAACCA 
ACTAGTGATG TTGTTGAAAC AAAAAGCTCT TCAAAGTTAA CAAATGATCC TGTCATTATT 
GGCGATTTTT CTGCCGCAAT GGTGCATACG ATAGTAAATG AAACTATAGA TTCACTGACA 
TCTTCCAAAG TTGCAAAAAC AGAAGAACAC ACAGATTGTT TAACTCAAAC AGGAAAGGGA 
AAAACCCCTT CTTTCTACTG GGATCAAGCA ACAGTGCCAC ACAGTGAGGC TAGAAAGAAG 
GACATGTTCG CTGATCGGTT ATCTAAATCT ATTATCAAAC ATTCCATAGA TGAAAGCAAG 
TCAGTGATCC TAAATGTAGA TAAAAATGTC CTCCCCAAGA AAGACTTGCC TGTTTCTGGA 
GATGAGTCAC AGTTGACCTT GGAAGAGTTC CCCAAGTTTC TTGAAGCTCA AGATCATTTA 
ACTCACTGTT CACTTTCAGA AGTAAAGGAT TGTGTTCCAG AAGGTAAAGG TTCTGTGGCT 
CCTGGATTTT CTTTGGAGAC GCTACCACCT TGTCTAGCAG CGGCAGGCCA GAAAGCTGAT 
CTGAAGGAAA TTGCTAAGGA CAAATCTGTG AAAAAGCAGA ATTTGCATAA TTCAGCCCTC 
GAGCCCTTGG CTTTTGGGCA GGAAAACTCT TTTCCCCATT CACATGCTTT CTCATCCACA 
GTGCTCACCT GTGTAGATGG TTTGCATGTG GAAGATAAAC AGAAAATGAG AGATGGGAAT 
GTAATACCTG ACACCCCTCC ATCAACTCCT CTGGTTCCAT CCCAGGCTCC TTCTGATTGG 
GATATCAAGA AGTTAACCAA AAAGCTTAAG GGGGAGTTAG CAAAAGAGTT TGCACCGGCC 
ACACCACCAT CCACACCTCA CAACTCATCT GTAAGCAGTT TGTCTGAAAA TGAACAAAAT 
GCAATAGAAA AAGAAGAGTT CATGTTGAAG CTCATGCGGT CTCTCTCAGA AGAAGTTGAA 
AGTAGTGACG GCGAAGAGCA GCAAGAGATG GACGTGAAAT TGGAGCCAGC AGGGAAAAAA 
GTTCAGTTTG CAGACGCATT AGCGACGCAC ATCGTTTCTC TTGCAACCGA AATGGCAGCT 
TCCCATTTGG ATAATAAATT GATTCAAGAA GCCGGGGTGA CAAGTCCTTG CTTAAATGTG 
CAGAGTCAAA GAAGCCTATC ACCTGCTTTT TTAAATTGCT CAGATGAAAG CTTACAAGCA 
TTATGCAGTT TTGCAGGTGA TATGGCAGCA GAAGTCATTA CAGAAGCTGA AAAAATAGCA 
AAAGTTAGGA GCTGTATGTT TTTCAGGAGG AAGAGGAACA GTTGTGTTGA TGGTGACCAA 
GATCGTAGAT CCGAAGAGAA GTTGGATGTA GAGGCTGTAG CGCACCCGGG AGGAGTAGAT 
CCGTTTGTTC TTCCTTTACC ACCAAGTTCT TGTCTGTCAG GTCTAACGTA CAAGTATCCC 
AGCTGTGAAA GTGTGACAGA TGAGTATGCA GGTCACATTA TCCAAATACT AAAACAGGAA 
GGTGGCAATA GTGAGTTAAT AATGGACCAG TACGCCAACA GACTTGCCTA TCGGTCTGTT 
AAGTCAGGAT TGCAAGAAGC AGCTAAAACA GCCAAAGTGA AGTGCAGCTC CAAAATACTC 
CCTGTGCAGA GCTCACAGGT GAAAACCAAC GATGAACTGT TACTGTTTTT AAATAAAGAA 
CACCAGCAAG AAGCAGATAA AAAAAGACAA AGCAGAAGTG CTGCAAGTTA CCTTCATAAA 
AACCGGACTT GTGAGTGGAC ACGGGACATG TACAGAAACG AGTGCTTGGA ACTACGTAGT 
TTTTCAACCT GTCTTGCTCA CAGCATCACA AGAGATGTTA AAAAAGAGTT GATGGCATCA 
ACAGGTGGCT TGCCAAAATC CTTAACAGAT TCTTGTCTTT ATGAAAAAAA CGTATGTGAC 
GAAGATACTG AGACTCATGT CGGGCCAGAG TTTCCCAAGT CTCTTCAGCC TTCTTCACAG 
AATCACAGAT TTTCTCAGAG TACAGGCAGC TTGAGTGGGT GTGGCTATGG AGAGACTGTC 
GTTCAAGCTA TAGAGCAGTA TGCTAACAAA GTCGTGGGCG ACACTTTAGA GCTGGGTTTA 
GGATCTGCCA TCTTCCACGT GTCTGAAGCC ACAAAAGCAG TTGATAGGAT TACTTACGCA 
GAAAAGCTGT CACCTCTGGT GGGTCAGGCT TGCCGATACT GTGACCGTAA AGAGCTCCAT 
GACTGCACTA GAAATTCGTC TCCTCACTTT CCCAGACCGG ATTCACTTGC TGGTAGTAAG 
CCAGTTTCTC ATTCACAGCT TAGCAGCTTC TATCAGAAAT CTAGGATTTT TCATCTTGAT 
GTTCCTCAAA TTCACGTTGA TCTCGATAAG AAGACAGTGC TTGCTGAGAA GATAGTTGCT 
GAAGCGATTG AAAAGGCAGA GAGAGAGCTG AGCAGTACCA GCCAGGCAGC CGACAGTGGG 
ATCGGACAGG AAGGTGTCAG CTTTGCCGAG AGCCTCAGCA CAGAGATACT GGCGTCAGCA 
GTGGTCAGTG CTGGACAGGC CATCAGCAGT CCAAAAGAAA CAGAAGAGAT TCAGTCAGCT 
GAGTCTCTTG GTAGCCAGCA GATGCATCTC AGTATTGGTG AGGACAGTAC TGGGAGCTGG 
TCCAACTTAA GTTTTGAAGA TGAACACCAG GATGAAAGCA GTAGTTTTCA CCATCTAAGT 
GAAAGTAATG GTAACAGCAG TAGCTGGAGC AGTCTTGGTT TAGAAGGAGA TTTGTATGAG 
GACAATTTAT CCTTTCCAAC ATCAGACAGT GATGGGCCAG ATGATGATGA AGAGGAGCGT 
CAGACTGATG TAGAAGGTTT GGAGCAAGAT GGAAAGACTC TGCTAATTAC AAATATTGAC 
ATGGAGCCAT GCACAGTAGA CCCCCAGCTA AGGATTATTC TTCAGTGGCT CGTTGCTTCT 
GAGGCAGAAG TTGCAGAACT TTATTTTCAT GACTCTACAA AGAAGGAGTT TATGCAACTT 
TCAAAACGGC TACAGGAAAA AGGGTGGAAG GTGGGAGACC TCCTGCAGGC TGTGCTTAAA 
TACTATGAGG AGGTGGAGAA GAAGTCCAGT GAGGAGAGAT GCAAGTCCCT GTTTGATTGG 
CTCTTGGAAA ATGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025118818.1
CDS	163..5880
Translation	MATFQSFRNNHMKTRASVRKSFNEDVFQSVKSLLQSEKELCSVSAEDCLKQDEHANLTEVTFLGFNEETDAAHIQDLAAVSLELPDLLNSLHFCSLNENEIICMKDINKSSDINSDPLNQSQHPGMLCVMRVSPTLPRLKIDFIFSLLSKYATGIRYTLDTYLHHKHQLEATNEDDDDTNQSVSSIEDDFVTAFEHLDEEETSKPYNDGLNIIALRSQCDAASQTISGHHLDTHDFRVLVGSEQQKSLAKPSASLINILGHKKLPPARTSVTTSVSEPWIQRSFYSSFAASEKGSDAQKPLFSSSPAYSSESECSSPSPVIFLDEEGYQKSLKAKLELPKIPVMKDDIEDSDSEVSEFFDSFDQFDELEQTLETACPFLKDLAVGKPSQKKGHKHEKLCSVTTTMNPQKFKFDRPALPANVRKPTPRKPESPYSNLCDAPDSPRPVKASGEDSGLFSPIRSSAFSPLGGCTPAECFCQTDIGGDRGHENPDSLYYTYEDYANSLSCEVLGSVLHSQHTNATSNTNSVKKGENKMVAFQHGSLDQKSKSKNKPIMIDSVQKFAADLVEKSFGSAFKDLQKGVSSCTNALCQLAIKLTSSIFQMAFNELRRQCAFSLKERAISGLASFLVSEAFSNALKDLQYVKKQIFTNTVTRFAADLAEELVFEGIMEVCQFSYPPTPASLQGRSFHCEDRVVKSYARDLSESVIQEAFIELSQVDVTFTTKAAVSVSPENIKFVSTEDVVPATQTSLCSSTSNSQTVTVTKPVQEYKKEYTVQQALFCTSGIVTSIPVPLAGSALHPYRISSNTYQTKSHPSCDDSHLNGGPTPASVATKNKEEELACLRNICLPSDHNPGSQNEVKPTSDVVETKSSSKLTNDPVIIGDFSAAMVHTIVNETIDSLTSSKVAKTEEHTDCLTQTGKGKTPSFYWDQATVPHSEARKKDMFADRLSKSIIKHSIDESKSVILNVDKNVLPKKDLPVSGDESQLTLEEFPKFLEAQDHLTHCSLSEVKDCVPEGKGSVAPGFSLETLPPCLAAAGQKADLKEIAKDKSVKKQNLHNSALEPLAFGQENSFPHSHAFSSTVLTCVDGLHVEDKQKMRDGNVIPDTPPSTPLVPSQAPSDWDIKKLTKKLKGELAKEFAPATPPSTPHNSSVSSLSENEQNAIEKEEFMLKLMRSLSEEVESSDGEEQQEMDVKLEPAGKKVQFADALATHIVSLATEMAASHLDNKLIQEAGVTSPCLNVQSQRSLSPAFLNCSDESLQALCSFAGDMAAEVITEAEKIAKVRSCMFFRRKRNSCVDGDQDRRSEEKLDVEAVAHPGGVDPFVLPLPPSSCLSGLTYKYPSCESVTDEYAGHIIQILKQEGGNSELIMDQYANRLAYRSVKSGLQEAAKTAKVKCSSKILPVQSSQVKTNDELLLFLNKEHQQEADKKRQSRSAASYLHKNRTCEWTRDMYRNECLELRSFSTCLAHSITRDVKKELMASTGGLPKSLTDSCLYEKNVCDEDTETHVGPEFPKSLQPSSQNHRFSQSTGSLSGCGYGETVVQAIEQYANKVVGDTLELGLGSAIFHVSEATKAVDRITYAEKLSPLVGQACRYCDRKELHDCTRNSSPHFPRPDSLAGSKPVSHSQLSSFYQKSRIFHLDVPQIHVDLDKKTVLAEKIVAEAIEKAERELSSTSQAADSGIGQEGVSFAESLSTEILASAVVSAGQAISSPKETEEIQSAESLGSQQMHLSIGEDSTGSWSNLSFEDEHQDESSSFHHLSESNGNSSSWSSLGLEGDLYEDNLSFPTSDSDGPDDDEEERQTDVEGLEQDGKTLLITNIDMEPCTVDPQLRIILQWLVASEAEVAELYFHDSTKKEFMQLSKRLQEKGWKVGDLLQAVLKYYEEVEKKSSEERCKSLFDWLLENA

Target ORF information:

RefSeq Version	XM_025263033.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025263033.1

ORF Insert Sequence:

ATGGCGACCT TCCAGTCTTT CAGAAACAAT CACATGAAAA CTAGAGCATC TGTCAGAAAA 
AGCTTCAATG AAGATGTGTT CCAGTCTGTG AAGTCTTTAT TGCAGAGCGA GAAGGAACTA 
TGCAGTGTAT CAGCCGAGGA CTGTTTGAAG CAGGATGAAC ATGCCAATTT GACTGAGGTC 
ACATTTCTAG GTTTTAATGA AGAAACAGAT GCTGCTCATA TACAGGATTT GGCTGCAGTT 
TCTTTGGAAC TCCCAGATCT TCTGAACTCA CTGCACTTCT GCAGTCTAAA TGAAAATGAA 
ATTATTTGTA TGAAGGATAT AAATAAATCA TCAGATATAA ACAGTGATCC TCTAAACCAG 
AGTCAACATC CTGGAATGCT TTGTGTCATG AGAGTGTCAC CTACATTACC AAGACTTAAA 
ATTGATTTTA TTTTTAGTCT CCTAAGTAAA TATGCTACCG GCATAAGGTA CACCCTGGAC 
ACATATTTGC ATCACAAGCA CCAACTTGAG GCCACCAATG AAGACGACGA TGATACTAAC 
CAGTCTGTAT CTTCCATCGA AGATGACTTT GTCACTGCTT TTGAGCACTT AGACGAGGAA 
GAGACTTCAA AGCCATATAA TGATGGATTA AACATAATTG CACTAAGGAG CCAGTGTGAT 
GCTGCTTCAC AGACTATTTC TGGTCACCAT TTGGATACCC ATGATTTCAG GGTTCTGGTT 
GGCTCTGAGC AGCAGAAGTC ATTGGCTAAA CCTTCAGCTT CTTTAATAAA CATTCTGGGA 
CATAAAAAAC TGCCTCCAGC GAGAACTTCA GTCACGACAT CAGTCTCTGA ACCATGGATC 
CAGAGGAGTT TCTATAGTTC CTTTGCTGCT TCAGAGAAAG GTAGTGATGC CCAGAAACCA 
TTGTTCTCGT CTTCTCCTGC CTACTCGTCT GAATCAGAGT GCTCAAGTCC AAGTCCTGTT 
ATTTTCTTGG ATGAAGAAGG TTATCAAAAA AGTTTGAAAG CAAAACTTGA GTTACCTAAA 
ATTCCTGTGA TGAAAGATGA TATTGAGGAT TCAGACTCAG AAGTGAGTGA GTTTTTTGAT 
AGTTTTGATC AGTTTGATGA ACTAGAACAA ACGTTAGAGA CTGCTTGTCC GTTTCTGAAA 
GATCTTGCTG TAGGGAAGCC ATCGCAAAAG AAAGGGCACA AACATGAAAA ATTGTGCTCT 
GTAACCACTA CTATGAATCC TCAAAAATTC AAGTTTGATC GGCCAGCTCT CCCAGCTAAT 
GTTAGGAAGC CAACACCTCG TAAACCAGAA TCCCCTTATA GTAACCTGTG TGATGCCCCA 
GATTCTCCCC GCCCCGTGAA GGCCTCAGGG GAAGACAGTG GTTTGTTTAG TCCTATTCGA 
TCCTCTGCTT TCAGTCCTCT TGGAGGCTGT ACTCCAGCTG AATGCTTTTG CCAAACAGAT 
ATTGGTGGAG ATAGGGGTCA TGAAAATCCT GATTCTCTTT ATTATACCTA TGAAGATTAT 
GCAAATAGCC TTTCCTGTGA AGTTCTGGGC TCAGTTCTTC ATAGCCAACA TACTAATGCA 
ACATCCAACA CTAATAGTGT TAAAAAGGGA GAAAATAAAA TGGTAGCTTT TCAGCATGGA 
AGCCTTGATC AAAAAAGTAA ATCTAAAAAT AAACCCATAA TGATTGATAG CGTTCAGAAG 
TTTGCAGCAG ATCTCGTGGA AAAAAGTTTT GGCAGTGCAT TTAAAGACTT ACAGAAAGGG 
GTCTCTTCAT GTACCAATGC CTTATGCCAG TTAGCCATCA AATTGACGTC ATCCATTTTT 
CAAATGGCAT TTAACGAACT GAGAAGGCAG TGTGCCTTTT CACTGAAAGA ACGAGCCATT 
AGTGGCCTGG CTAGTTTTTT GGTGAGTGAG GCATTCTCAA ATGCTTTAAA AGATCTACAA 
TATGTAAAGA AACAGATCTT CACTAACACC GTCACTAGAT TTGCTGCAGA TCTTGCTGAA 
GAGCTTGTTT TTGAAGGCAT CATGGAAGTG TGTCAGTTCT CATATCCTCC AACACCTGCA 
TCTCTGCAGG GTCGGTCATT TCACTGTGAA GACAGAGTGG TGAAGTCCTA TGCAAGAGAT 
CTGTCTGAAT CTGTAATACA GGAAGCATTC ATTGAGCTGT CTCAAGTTGA TGTTACCTTT 
ACCACAAAGG CAGCAGTTAG TGTTTCCCCA GAAAACATCA AGTTTGTGAG CACAGAAGAT 
GTAGTGCCAG CAACACAGAC TTCTTTGTGT TCTTCTACTT CTAACAGTCA AACAGTTACG 
GTGACAAAAC CAGTGCAGGA GTATAAAAAG GAATACACGG TGCAGCAGGC CTTGTTCTGT 
ACTTCAGGAA TTGTTACTTC AATACCAGTG CCCTTGGCAG GAAGTGCCCT TCACCCATAC 
CGTATTTCTT CTAATACGTA TCAGACAAAG TCTCATCCAT CATGTGATGA TAGTCATTTG 
AATGGTGGTC CTACCCCAGC AAGTGTTGCC ACAAAAAACA AAGAAGAGGA GCTAGCTTGT 
CTCAGGAATA TTTGTTTACC TTCAGACCAC AACCCCGGTA GCCAGAATGA GGTGAAACCA 
ACTAGTGATG TTGTTGAAAC AAAAAGCTCT TCAAAGTTAA CAAATGATCC TGTCATTATT 
GGCGATTTTT CTGCCGCAAT GGTGCATACG ATAGTAAATG AAACTATAGA TTCACTGACA 
TCTTCCAAAG TTGCAAAAAC AGAAGAACAC ACAGATTGTT TAACTCAAAC AGGAAAGGGA 
AAAACCCCTT CTTTCTACTG GGATCAAGCA ACAGTGCCAC ACAGTGAGGC TAGAAAGAAG 
GACATGTTCG CTGATCGGTT ATCTAAATCT ATTATCAAAC ATTCCATAGA TGAAAGCAAG 
TCAGTGATCC TAAATGTAGA TAAAAATGTC CTCCCCAAGA AAGACTTGCC TGTTTCTGGA 
GATGAGTCAC AGTTGACCTT GGAAGAGTTC CCCAAGTTTC TTGAAGCTCA AGATCATTTA 
ACTCACTGTT CACTTTCAGA AGTAAAGGAT TGTGTTCCAG AAGGTAAAGG TTCTGTGGCT 
CCTGGATTTT CTTTGGAGAC GCTACCACCT TGTCTAGCAG CGGCAGGCCA GAAAGCTGAT 
CTGAAGGAAA TTGCTAAGGA CAAATCTGTG AAAAAGCAGA ATTTGCATAA TTCAGCCCTC 
GAGCCCTTGG CTTTTGGGCA GGAAAACTCT TTTCCCCATT CACATGCTTT CTCATCCACA 
GTGCTCACCT GTGTAGATGG TTTGCATGTG GAAGATAAAC AGAAAATGAG AGATGGGAAT 
GTAATACCTG ACACCCCTCC ATCAACTCCT CTGGTTCCAT CCCAGGCTCC TTCTGATTGG 
GATATCAAGA AGTTAACCAA AAAGCTTAAG GGGGAGTTAG CAAAAGAGTT TGCACCGGCC 
ACACCACCAT CCACACCTCA CAACTCATCT GTAAGCAGTT TGTCTGAAAA TGAACAAAAT 
GCAATAGAAA AAGAAGAGTT CATGTTGAAG CTCATGCGGT CTCTCTCAGA AGAAGTTGAA 
AGTAGTGACG GCGAAGAGCA GCAAGAGATG GACGTGAAAT TGGAGCCAGC AGGGAAAAAA 
GTTCAGTTTG CAGACGCATT AGCGACGCAC ATCGTTTCTC TTGCAACCGA AATGGCAGCT 
TCCCATTTGG ATAATAAATT GATTCAAGAA GCCGGGGTGA CAAGTCCTTG CTTAAATGTG 
CAGAGTCAAA GAAGCCTATC ACCTGCTTTT TTAAATTGCT CAGATGAAAG CTTACAAGCA 
TTATGCAGTT TTGCAGGTGA TATGGCAGCA GAAGTCATTA CAGAAGCTGA AAAAATAGCA 
AAAGTTAGGA GCTGTATGTT TTTCAGGAGG AAGAGGAACA GTTGTGTTGA TGGTGACCAA 
GATCGTAGAT CCGAAGAGAA GTTGGATGTA GAGGCTGTAG CGCACCCGGG AGGAGTAGAT 
CCGTTTGTTC TTCCTTTACC ACCAAGTTCT TGTCTGTCAG GTCTAACGTA CAAGTATCCC 
AGCTGTGAAA GTGTGACAGA TGAGTATGCA GGTCACATTA TCCAAATACT AAAACAGGAA 
GGTGGCAATA GTGAGTTAAT AATGGACCAG TACGCCAACA GACTTGCCTA TCGGTCTGTT 
AAGTCAGGAT TGCAAGAAGC AGCTAAAACA GCCAAAGTGA AGTGCAGCTC CAAAATACTC 
CCTGTGCAGA GCTCACAGGT GAAAACCAAC GATGAACTGT TACTGTTTTT AAATAAAGAA 
CACCAGCAAG AAGCAGATAA AAAAAGACAA AGCAGAAGTG CTGCAAGTTA CCTTCATAAA 
AACCGGACTT GTGAGTGGAC ACGGGACATG TACAGAAACG AGTGCTTGGA ACTACGTAGT 
TTTTCAACCT GTCTTGCTCA CAGCATCACA AGAGATGTTA AAAAAGAGTT GATGGCATCA 
ACAGGTGGCT TGCCAAAATC CTTAACAGAT TCTTGTCTTT ATGAAAAAAA CGTATGTGAC 
GAAGATACTG AGACTCATGT CGGGCCAGAG TTTCCCAAGT CTCTTCAGCC TTCTTCACAG 
AATCACAGAT TTTCTCAGAG TACAGGCAGC TTGAGTGGGT GTGGCTATGG AGAGACTGTC 
GTTCAAGCTA TAGAGCAGTA TGCTAACAAA GTCGTGGGCG ACACTTTAGA GCTGGGTTTA 
GGATCTGCCA TCTTCCACGT GTCTGAAGCC ACAAAAGCAG TTGATAGGAT TACTTACGCA 
GAAAAGCTGT CACCTCTGGT GGGTCAGGCT TGCCGATACT GTGACCGTAA AGAGCTCCAT 
GACTGCACTA GAAATTCGTC TCCTCACTTT CCCAGACCGG ATTCACTTGC TGGTAGTAAG 
CCAGTTTCTC ATTCACAGCT TAGCAGCTTC TATCAGAAAT CTAGGATTTT TCATCTTGAT 
GTTCCTCAAA TTCACGTTGA TCTCGATAAG AAGACAGTGC TTGCTGAGAA GATAGTTGCT 
GAAGCGATTG AAAAGGCAGA GAGAGAGCTG AGCAGTACCA GCCAGGCAGC CGACAGTGGG 
ATCGGACAGG AAGGTGTCAG CTTTGCCGAG AGCCTCAGCA CAGAGATACT GGCGTCAGCA 
GTGGTCAGTG CTGGACAGGC CATCAGCAGT CCAAAAGAAA CAGAAGAGAT TCAGTCAGCT 
GAGTCTCTTG GTAGCCAGCA GATGCATCTC AGTATTGGTG AGGACAGTAC TGGGAGCTGG 
TCCAACTTAA GTTTTGAAGA TGAACACCAG GATGAAAGCA GTAGTTTTCA CCATCTAAGT 
GAAAGTAATG GTAACAGCAG TAGCTGGAGC AGTCTTGGTT TAGAAGGAGA TTTGTATGAG 
GACAATTTAT CCTTTCCAAC ATCAGACAGT GATGGGCCAG ATGATGATGA AGAGGAGCGT 
CAGACTGATG TAGAAGGTTT GGAGCAAGAT GGAAAGACTC TGCTAATTAC AAATATTGAC 
ATGGAGCCAT GCACAGTAGA CCCCCAGCTA AGGATTATTC TTCAGTGGCT CGTTGCTTCT 
GAGGCAGAAG TTGCAGAACT TTATTTTCAT GACTCTACAA AGAAGGAGTT TATGCAACTT 
TCAAAACGGC TACAGGAAAA AGGGTGGAAG GTGGGAGACC TCCTGCAGGC TGTGCTTAAA 
TACTATGAGG AGGTGGAGAA GAAGTCCAGT GAGGAGAGAT GCAAGTCCCT GTTTGATTGG 
CTCTTGGAAA ATGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP11 ( XM_025263034.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025118819.1 Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X3, mRNA.

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CloneID	OBu30010
Clone ID Related Accession (Same CDS sequence)	XM_006076502.2 , XM_025263033.1 , XM_025263034.1 , XM_006076502.1
Accession Version	XM_025263034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	A-kinase anchor protein 11 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCT TCCAGTCTTT CAGAAACAAT CACATGAAAA CTAGAGCATC TGTCAGAAAA 
AGCTTCAATG AAGATGTGTT CCAGTCTGTG AAGTCTTTAT TGCAGAGCGA GAAGGAACTA 
TGCAGTGTAT CAGCCGAGGA CTGTTTGAAG CAGGATGAAC ATGCCAATTT GACTGAGGTC 
ACATTTCTAG GTTTTAATGA AGAAACAGAT GCTGCTCATA TACAGGATTT GGCTGCAGTT 
TCTTTGGAAC TCCCAGATCT TCTGAACTCA CTGCACTTCT GCAGTCTAAA TGAAAATGAA 
ATTATTTGTA TGAAGGATAT AAATAAATCA TCAGATATAA ACAGTGATCC TCTAAACCAG 
AGTCAACATC CTGGAATGCT TTGTGTCATG AGAGTGTCAC CTACATTACC AAGACTTAAA 
ATTGATTTTA TTTTTAGTCT CCTAAGTAAA TATGCTACCG GCATAAGGTA CACCCTGGAC 
ACATATTTGC ATCACAAGCA CCAACTTGAG GCCACCAATG AAGACGACGA TGATACTAAC 
CAGTCTGTAT CTTCCATCGA AGATGACTTT GTCACTGCTT TTGAGCACTT AGACGAGGAA 
GAGACTTCAA AGCCATATAA TGATGGATTA AACATAATTG CACTAAGGAG CCAGTGTGAT 
GCTGCTTCAC AGACTATTTC TGGTCACCAT TTGGATACCC ATGATTTCAG GGTTCTGGTT 
GGCTCTGAGC AGCAGAAGTC ATTGGCTAAA CCTTCAGCTT CTTTAATAAA CATTCTGGGA 
CATAAAAAAC TGCCTCCAGC GAGAACTTCA GTCACGACAT CAGTCTCTGA ACCATGGATC 
CAGAGGAGTT TCTATAGTTC CTTTGCTGCT TCAGAGAAAG GTAGTGATGC CCAGAAACCA 
TTGTTCTCGT CTTCTCCTGC CTACTCGTCT GAATCAGAGT GCTCAAGTCC AAGTCCTGTT 
ATTTTCTTGG ATGAAGAAGG TTATCAAAAA AGTTTGAAAG CAAAACTTGA GTTACCTAAA 
ATTCCTGTGA TGAAAGATGA TATTGAGGAT TCAGACTCAG AAGTGAGTGA GTTTTTTGAT 
AGTTTTGATC AGTTTGATGA ACTAGAACAA ACGTTAGAGA CTGCTTGTCC GTTTCTGAAA 
GATCTTGCTG TAGGGAAGCC ATCGCAAAAG AAAGGGCACA AACATGAAAA ATTGTGCTCT 
GTAACCACTA CTATGAATCC TCAAAAATTC AAGTTTGATC GGCCAGCTCT CCCAGCTAAT 
GTTAGGAAGC CAACACCTCG TAAACCAGAA TCCCCTTATA GTAACCTGTG TGATGCCCCA 
GATTCTCCCC GCCCCGTGAA GGCCTCAGGG GAAGACAGTG GTTTGTTTAG TCCTATTCGA 
TCCTCTGCTT TCAGTCCTCT TGGAGGCTGT ACTCCAGCTG AATGCTTTTG CCAAACAGAT 
ATTGGTGGAG ATAGGGGTCA TGAAAATCCT GATTCTCTTT ATTATACCTA TGAAGATTAT 
GCAAATAGCC TTTCCTGTGA AGTTCTGGGC TCAGTTCTTC ATAGCCAACA TACTAATGCA 
ACATCCAACA CTAATAGTGT TAAAAAGGGA GAAAATAAAA TGGTAGCTTT TCAGCATGGA 
AGCCTTGATC AAAAAAGTAA ATCTAAAAAT AAACCCATAA TGATTGATAG CGTTCAGAAG 
TTTGCAGCAG ATCTCGTGGA AAAAAGTTTT GGCAGTGCAT TTAAAGACTT ACAGAAAGGG 
GTCTCTTCAT GTACCAATGC CTTATGCCAG TTAGCCATCA AATTGACGTC ATCCATTTTT 
CAAATGGCAT TTAACGAACT GAGAAGGCAG TGTGCCTTTT CACTGAAAGA ACGAGCCATT 
AGTGGCCTGG CTAGTTTTTT GGTGAGTGAG GCATTCTCAA ATGCTTTAAA AGATCTACAA 
TATGTAAAGA AACAGATCTT CACTAACACC GTCACTAGAT TTGCTGCAGA TCTTGCTGAA 
GAGCTTGTTT TTGAAGGCAT CATGGAAGTG TGTCAGTTCT CATATCCTCC AACACCTGCA 
TCTCTGCAGG GTCGGTCATT TCACTGTGAA GACAGAGTGG TGAAGTCCTA TGCAAGAGAT 
CTGTCTGAAT CTGTAATACA GGAAGCATTC ATTGAGCTGT CTCAAGTTGA TGTTACCTTT 
ACCACAAAGG CAGCAGTTAG TGTTTCCCCA GAAAACATCA AGTTTGTGAG CACAGAAGAT 
GTAGTGCCAG CAACACAGAC TTCTTTGTGT TCTTCTACTT CTAACAGTCA AACAGTTACG 
GTGACAAAAC CAGTGCAGGA GTATAAAAAG GAATACACGG TGCAGCAGGC CTTGTTCTGT 
ACTTCAGGAA TTGTTACTTC AATACCAGTG CCCTTGGCAG GAAGTGCCCT TCACCCATAC 
CGTATTTCTT CTAATACGTA TCAGACAAAG TCTCATCCAT CATGTGATGA TAGTCATTTG 
AATGGTGGTC CTACCCCAGC AAGTGTTGCC ACAAAAAACA AAGAAGAGGA GCTAGCTTGT 
CTCAGGAATA TTTGTTTACC TTCAGACCAC AACCCCGGTA GCCAGAATGA GGTGAAACCA 
ACTAGTGATG TTGTTGAAAC AAAAAGCTCT TCAAAGTTAA CAAATGATCC TGTCATTATT 
GGCGATTTTT CTGCCGCAAT GGTGCATACG ATAGTAAATG AAACTATAGA TTCACTGACA 
TCTTCCAAAG TTGCAAAAAC AGAAGAACAC ACAGATTGTT TAACTCAAAC AGGAAAGGGA 
AAAACCCCTT CTTTCTACTG GGATCAAGCA ACAGTGCCAC ACAGTGAGGC TAGAAAGAAG 
GACATGTTCG CTGATCGGTT ATCTAAATCT ATTATCAAAC ATTCCATAGA TGAAAGCAAG 
TCAGTGATCC TAAATGTAGA TAAAAATGTC CTCCCCAAGA AAGACTTGCC TGTTTCTGGA 
GATGAGTCAC AGTTGACCTT GGAAGAGTTC CCCAAGTTTC TTGAAGCTCA AGATCATTTA 
ACTCACTGTT CACTTTCAGA AGTAAAGGAT TGTGTTCCAG AAGGTAAAGG TTCTGTGGCT 
CCTGGATTTT CTTTGGAGAC GCTACCACCT TGTCTAGCAG CGGCAGGCCA GAAAGCTGAT 
CTGAAGGAAA TTGCTAAGGA CAAATCTGTG AAAAAGCAGA ATTTGCATAA TTCAGCCCTC 
GAGCCCTTGG CTTTTGGGCA GGAAAACTCT TTTCCCCATT CACATGCTTT CTCATCCACA 
GTGCTCACCT GTGTAGATGG TTTGCATGTG GAAGATAAAC AGAAAATGAG AGATGGGAAT 
GTAATACCTG ACACCCCTCC ATCAACTCCT CTGGTTCCAT CCCAGGCTCC TTCTGATTGG 
GATATCAAGA AGTTAACCAA AAAGCTTAAG GGGGAGTTAG CAAAAGAGTT TGCACCGGCC 
ACACCACCAT CCACACCTCA CAACTCATCT GTAAGCAGTT TGTCTGAAAA TGAACAAAAT 
GCAATAGAAA AAGAAGAGTT CATGTTGAAG CTCATGCGGT CTCTCTCAGA AGAAGTTGAA 
AGTAGTGACG GCGAAGAGCA GCAAGAGATG GACGTGAAAT TGGAGCCAGC AGGGAAAAAA 
GTTCAGTTTG CAGACGCATT AGCGACGCAC ATCGTTTCTC TTGCAACCGA AATGGCAGCT 
TCCCATTTGG ATAATAAATT GATTCAAGAA GCCGGGGTGA CAAGTCCTTG CTTAAATGTG 
CAGAGTCAAA GAAGCCTATC ACCTGCTTTT TTAAATTGCT CAGATGAAAG CTTACAAGCA 
TTATGCAGTT TTGCAGGTGA TATGGCAGCA GAAGTCATTA CAGAAGCTGA AAAAATAGCA 
AAAGTTAGGA GCTGTATGTT TTTCAGGAGG AAGAGGAACA GTTGTGTTGA TGGTGACCAA 
GATCGTAGAT CCGAAGAGAA GTTGGATGTA GAGGCTGTAG CGCACCCGGG AGGAGTAGAT 
CCGTTTGTTC TTCCTTTACC ACCAAGTTCT TGTCTGTCAG GTCTAACGTA CAAGTATCCC 
AGCTGTGAAA GTGTGACAGA TGAGTATGCA GGTCACATTA TCCAAATACT AAAACAGGAA 
GGTGGCAATA GTGAGTTAAT AATGGACCAG TACGCCAACA GACTTGCCTA TCGGTCTGTT 
AAGTCAGGAT TGCAAGAAGC AGCTAAAACA GCCAAAGTGA AGTGCAGCTC CAAAATACTC 
CCTGTGCAGA GCTCACAGGT GAAAACCAAC GATGAACTGT TACTGTTTTT AAATAAAGAA 
CACCAGCAAG AAGCAGATAA AAAAAGACAA AGCAGAAGTG CTGCAAGTTA CCTTCATAAA 
AACCGGACTT GTGAGTGGAC ACGGGACATG TACAGAAACG AGTGCTTGGA ACTACGTAGT 
TTTTCAACCT GTCTTGCTCA CAGCATCACA AGAGATGTTA AAAAAGAGTT GATGGCATCA 
ACAGGTGGCT TGCCAAAATC CTTAACAGAT TCTTGTCTTT ATGAAAAAAA CGTATGTGAC 
GAAGATACTG AGACTCATGT CGGGCCAGAG TTTCCCAAGT CTCTTCAGCC TTCTTCACAG 
AATCACAGAT TTTCTCAGAG TACAGGCAGC TTGAGTGGGT GTGGCTATGG AGAGACTGTC 
GTTCAAGCTA TAGAGCAGTA TGCTAACAAA GTCGTGGGCG ACACTTTAGA GCTGGGTTTA 
GGATCTGCCA TCTTCCACGT GTCTGAAGCC ACAAAAGCAG TTGATAGGAT TACTTACGCA 
GAAAAGCTGT CACCTCTGGT GGGTCAGGCT TGCCGATACT GTGACCGTAA AGAGCTCCAT 
GACTGCACTA GAAATTCGTC TCCTCACTTT CCCAGACCGG ATTCACTTGC TGGTAGTAAG 
CCAGTTTCTC ATTCACAGCT TAGCAGCTTC TATCAGAAAT CTAGGATTTT TCATCTTGAT 
GTTCCTCAAA TTCACGTTGA TCTCGATAAG AAGACAGTGC TTGCTGAGAA GATAGTTGCT 
GAAGCGATTG AAAAGGCAGA GAGAGAGCTG AGCAGTACCA GCCAGGCAGC CGACAGTGGG 
ATCGGACAGG AAGGTGTCAG CTTTGCCGAG AGCCTCAGCA CAGAGATACT GGCGTCAGCA 
GTGGTCAGTG CTGGACAGGC CATCAGCAGT CCAAAAGAAA CAGAAGAGAT TCAGTCAGCT 
GAGTCTCTTG GTAGCCAGCA GATGCATCTC AGTATTGGTG AGGACAGTAC TGGGAGCTGG 
TCCAACTTAA GTTTTGAAGA TGAACACCAG GATGAAAGCA GTAGTTTTCA CCATCTAAGT 
GAAAGTAATG GTAACAGCAG TAGCTGGAGC AGTCTTGGTT TAGAAGGAGA TTTGTATGAG 
GACAATTTAT CCTTTCCAAC ATCAGACAGT GATGGGCCAG ATGATGATGA AGAGGAGCGT 
CAGACTGATG TAGAAGGTTT GGAGCAAGAT GGAAAGACTC TGCTAATTAC AAATATTGAC 
ATGGAGCCAT GCACAGTAGA CCCCCAGCTA AGGATTATTC TTCAGTGGCT CGTTGCTTCT 
GAGGCAGAAG TTGCAGAACT TTATTTTCAT GACTCTACAA AGAAGGAGTT TATGCAACTT 
TCAAAACGGC TACAGGAAAA AGGGTGGAAG GTGGGAGACC TCCTGCAGGC TGTGCTTAAA 
TACTATGAGG AGGTGGAGAA GAAGTCCAGT GAGGAGAGAT GCAAGTCCCT GTTTGATTGG 
CTCTTGGAAA ATGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025118819.1
CDS	282..5999
Translation	MATFQSFRNNHMKTRASVRKSFNEDVFQSVKSLLQSEKELCSVSAEDCLKQDEHANLTEVTFLGFNEETDAAHIQDLAAVSLELPDLLNSLHFCSLNENEIICMKDINKSSDINSDPLNQSQHPGMLCVMRVSPTLPRLKIDFIFSLLSKYATGIRYTLDTYLHHKHQLEATNEDDDDTNQSVSSIEDDFVTAFEHLDEEETSKPYNDGLNIIALRSQCDAASQTISGHHLDTHDFRVLVGSEQQKSLAKPSASLINILGHKKLPPARTSVTTSVSEPWIQRSFYSSFAASEKGSDAQKPLFSSSPAYSSESECSSPSPVIFLDEEGYQKSLKAKLELPKIPVMKDDIEDSDSEVSEFFDSFDQFDELEQTLETACPFLKDLAVGKPSQKKGHKHEKLCSVTTTMNPQKFKFDRPALPANVRKPTPRKPESPYSNLCDAPDSPRPVKASGEDSGLFSPIRSSAFSPLGGCTPAECFCQTDIGGDRGHENPDSLYYTYEDYANSLSCEVLGSVLHSQHTNATSNTNSVKKGENKMVAFQHGSLDQKSKSKNKPIMIDSVQKFAADLVEKSFGSAFKDLQKGVSSCTNALCQLAIKLTSSIFQMAFNELRRQCAFSLKERAISGLASFLVSEAFSNALKDLQYVKKQIFTNTVTRFAADLAEELVFEGIMEVCQFSYPPTPASLQGRSFHCEDRVVKSYARDLSESVIQEAFIELSQVDVTFTTKAAVSVSPENIKFVSTEDVVPATQTSLCSSTSNSQTVTVTKPVQEYKKEYTVQQALFCTSGIVTSIPVPLAGSALHPYRISSNTYQTKSHPSCDDSHLNGGPTPASVATKNKEEELACLRNICLPSDHNPGSQNEVKPTSDVVETKSSSKLTNDPVIIGDFSAAMVHTIVNETIDSLTSSKVAKTEEHTDCLTQTGKGKTPSFYWDQATVPHSEARKKDMFADRLSKSIIKHSIDESKSVILNVDKNVLPKKDLPVSGDESQLTLEEFPKFLEAQDHLTHCSLSEVKDCVPEGKGSVAPGFSLETLPPCLAAAGQKADLKEIAKDKSVKKQNLHNSALEPLAFGQENSFPHSHAFSSTVLTCVDGLHVEDKQKMRDGNVIPDTPPSTPLVPSQAPSDWDIKKLTKKLKGELAKEFAPATPPSTPHNSSVSSLSENEQNAIEKEEFMLKLMRSLSEEVESSDGEEQQEMDVKLEPAGKKVQFADALATHIVSLATEMAASHLDNKLIQEAGVTSPCLNVQSQRSLSPAFLNCSDESLQALCSFAGDMAAEVITEAEKIAKVRSCMFFRRKRNSCVDGDQDRRSEEKLDVEAVAHPGGVDPFVLPLPPSSCLSGLTYKYPSCESVTDEYAGHIIQILKQEGGNSELIMDQYANRLAYRSVKSGLQEAAKTAKVKCSSKILPVQSSQVKTNDELLLFLNKEHQQEADKKRQSRSAASYLHKNRTCEWTRDMYRNECLELRSFSTCLAHSITRDVKKELMASTGGLPKSLTDSCLYEKNVCDEDTETHVGPEFPKSLQPSSQNHRFSQSTGSLSGCGYGETVVQAIEQYANKVVGDTLELGLGSAIFHVSEATKAVDRITYAEKLSPLVGQACRYCDRKELHDCTRNSSPHFPRPDSLAGSKPVSHSQLSSFYQKSRIFHLDVPQIHVDLDKKTVLAEKIVAEAIEKAERELSSTSQAADSGIGQEGVSFAESLSTEILASAVVSAGQAISSPKETEEIQSAESLGSQQMHLSIGEDSTGSWSNLSFEDEHQDESSSFHHLSESNGNSSSWSSLGLEGDLYEDNLSFPTSDSDGPDDDEEERQTDVEGLEQDGKTLLITNIDMEPCTVDPQLRIILQWLVASEAEVAELYFHDSTKKEFMQLSKRLQEKGWKVGDLLQAVLKYYEEVEKKSSEERCKSLFDWLLENA

Target ORF information:

RefSeq Version	XM_025263034.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025263034.1

ORF Insert Sequence:

ATGGCGACCT TCCAGTCTTT CAGAAACAAT CACATGAAAA CTAGAGCATC TGTCAGAAAA 
AGCTTCAATG AAGATGTGTT CCAGTCTGTG AAGTCTTTAT TGCAGAGCGA GAAGGAACTA 
TGCAGTGTAT CAGCCGAGGA CTGTTTGAAG CAGGATGAAC ATGCCAATTT GACTGAGGTC 
ACATTTCTAG GTTTTAATGA AGAAACAGAT GCTGCTCATA TACAGGATTT GGCTGCAGTT 
TCTTTGGAAC TCCCAGATCT TCTGAACTCA CTGCACTTCT GCAGTCTAAA TGAAAATGAA 
ATTATTTGTA TGAAGGATAT AAATAAATCA TCAGATATAA ACAGTGATCC TCTAAACCAG 
AGTCAACATC CTGGAATGCT TTGTGTCATG AGAGTGTCAC CTACATTACC AAGACTTAAA 
ATTGATTTTA TTTTTAGTCT CCTAAGTAAA TATGCTACCG GCATAAGGTA CACCCTGGAC 
ACATATTTGC ATCACAAGCA CCAACTTGAG GCCACCAATG AAGACGACGA TGATACTAAC 
CAGTCTGTAT CTTCCATCGA AGATGACTTT GTCACTGCTT TTGAGCACTT AGACGAGGAA 
GAGACTTCAA AGCCATATAA TGATGGATTA AACATAATTG CACTAAGGAG CCAGTGTGAT 
GCTGCTTCAC AGACTATTTC TGGTCACCAT TTGGATACCC ATGATTTCAG GGTTCTGGTT 
GGCTCTGAGC AGCAGAAGTC ATTGGCTAAA CCTTCAGCTT CTTTAATAAA CATTCTGGGA 
CATAAAAAAC TGCCTCCAGC GAGAACTTCA GTCACGACAT CAGTCTCTGA ACCATGGATC 
CAGAGGAGTT TCTATAGTTC CTTTGCTGCT TCAGAGAAAG GTAGTGATGC CCAGAAACCA 
TTGTTCTCGT CTTCTCCTGC CTACTCGTCT GAATCAGAGT GCTCAAGTCC AAGTCCTGTT 
ATTTTCTTGG ATGAAGAAGG TTATCAAAAA AGTTTGAAAG CAAAACTTGA GTTACCTAAA 
ATTCCTGTGA TGAAAGATGA TATTGAGGAT TCAGACTCAG AAGTGAGTGA GTTTTTTGAT 
AGTTTTGATC AGTTTGATGA ACTAGAACAA ACGTTAGAGA CTGCTTGTCC GTTTCTGAAA 
GATCTTGCTG TAGGGAAGCC ATCGCAAAAG AAAGGGCACA AACATGAAAA ATTGTGCTCT 
GTAACCACTA CTATGAATCC TCAAAAATTC AAGTTTGATC GGCCAGCTCT CCCAGCTAAT 
GTTAGGAAGC CAACACCTCG TAAACCAGAA TCCCCTTATA GTAACCTGTG TGATGCCCCA 
GATTCTCCCC GCCCCGTGAA GGCCTCAGGG GAAGACAGTG GTTTGTTTAG TCCTATTCGA 
TCCTCTGCTT TCAGTCCTCT TGGAGGCTGT ACTCCAGCTG AATGCTTTTG CCAAACAGAT 
ATTGGTGGAG ATAGGGGTCA TGAAAATCCT GATTCTCTTT ATTATACCTA TGAAGATTAT 
GCAAATAGCC TTTCCTGTGA AGTTCTGGGC TCAGTTCTTC ATAGCCAACA TACTAATGCA 
ACATCCAACA CTAATAGTGT TAAAAAGGGA GAAAATAAAA TGGTAGCTTT TCAGCATGGA 
AGCCTTGATC AAAAAAGTAA ATCTAAAAAT AAACCCATAA TGATTGATAG CGTTCAGAAG 
TTTGCAGCAG ATCTCGTGGA AAAAAGTTTT GGCAGTGCAT TTAAAGACTT ACAGAAAGGG 
GTCTCTTCAT GTACCAATGC CTTATGCCAG TTAGCCATCA AATTGACGTC ATCCATTTTT 
CAAATGGCAT TTAACGAACT GAGAAGGCAG TGTGCCTTTT CACTGAAAGA ACGAGCCATT 
AGTGGCCTGG CTAGTTTTTT GGTGAGTGAG GCATTCTCAA ATGCTTTAAA AGATCTACAA 
TATGTAAAGA AACAGATCTT CACTAACACC GTCACTAGAT TTGCTGCAGA TCTTGCTGAA 
GAGCTTGTTT TTGAAGGCAT CATGGAAGTG TGTCAGTTCT CATATCCTCC AACACCTGCA 
TCTCTGCAGG GTCGGTCATT TCACTGTGAA GACAGAGTGG TGAAGTCCTA TGCAAGAGAT 
CTGTCTGAAT CTGTAATACA GGAAGCATTC ATTGAGCTGT CTCAAGTTGA TGTTACCTTT 
ACCACAAAGG CAGCAGTTAG TGTTTCCCCA GAAAACATCA AGTTTGTGAG CACAGAAGAT 
GTAGTGCCAG CAACACAGAC TTCTTTGTGT TCTTCTACTT CTAACAGTCA AACAGTTACG 
GTGACAAAAC CAGTGCAGGA GTATAAAAAG GAATACACGG TGCAGCAGGC CTTGTTCTGT 
ACTTCAGGAA TTGTTACTTC AATACCAGTG CCCTTGGCAG GAAGTGCCCT TCACCCATAC 
CGTATTTCTT CTAATACGTA TCAGACAAAG TCTCATCCAT CATGTGATGA TAGTCATTTG 
AATGGTGGTC CTACCCCAGC AAGTGTTGCC ACAAAAAACA AAGAAGAGGA GCTAGCTTGT 
CTCAGGAATA TTTGTTTACC TTCAGACCAC AACCCCGGTA GCCAGAATGA GGTGAAACCA 
ACTAGTGATG TTGTTGAAAC AAAAAGCTCT TCAAAGTTAA CAAATGATCC TGTCATTATT 
GGCGATTTTT CTGCCGCAAT GGTGCATACG ATAGTAAATG AAACTATAGA TTCACTGACA 
TCTTCCAAAG TTGCAAAAAC AGAAGAACAC ACAGATTGTT TAACTCAAAC AGGAAAGGGA 
AAAACCCCTT CTTTCTACTG GGATCAAGCA ACAGTGCCAC ACAGTGAGGC TAGAAAGAAG 
GACATGTTCG CTGATCGGTT ATCTAAATCT ATTATCAAAC ATTCCATAGA TGAAAGCAAG 
TCAGTGATCC TAAATGTAGA TAAAAATGTC CTCCCCAAGA AAGACTTGCC TGTTTCTGGA 
GATGAGTCAC AGTTGACCTT GGAAGAGTTC CCCAAGTTTC TTGAAGCTCA AGATCATTTA 
ACTCACTGTT CACTTTCAGA AGTAAAGGAT TGTGTTCCAG AAGGTAAAGG TTCTGTGGCT 
CCTGGATTTT CTTTGGAGAC GCTACCACCT TGTCTAGCAG CGGCAGGCCA GAAAGCTGAT 
CTGAAGGAAA TTGCTAAGGA CAAATCTGTG AAAAAGCAGA ATTTGCATAA TTCAGCCCTC 
GAGCCCTTGG CTTTTGGGCA GGAAAACTCT TTTCCCCATT CACATGCTTT CTCATCCACA 
GTGCTCACCT GTGTAGATGG TTTGCATGTG GAAGATAAAC AGAAAATGAG AGATGGGAAT 
GTAATACCTG ACACCCCTCC ATCAACTCCT CTGGTTCCAT CCCAGGCTCC TTCTGATTGG 
GATATCAAGA AGTTAACCAA AAAGCTTAAG GGGGAGTTAG CAAAAGAGTT TGCACCGGCC 
ACACCACCAT CCACACCTCA CAACTCATCT GTAAGCAGTT TGTCTGAAAA TGAACAAAAT 
GCAATAGAAA AAGAAGAGTT CATGTTGAAG CTCATGCGGT CTCTCTCAGA AGAAGTTGAA 
AGTAGTGACG GCGAAGAGCA GCAAGAGATG GACGTGAAAT TGGAGCCAGC AGGGAAAAAA 
GTTCAGTTTG CAGACGCATT AGCGACGCAC ATCGTTTCTC TTGCAACCGA AATGGCAGCT 
TCCCATTTGG ATAATAAATT GATTCAAGAA GCCGGGGTGA CAAGTCCTTG CTTAAATGTG 
CAGAGTCAAA GAAGCCTATC ACCTGCTTTT TTAAATTGCT CAGATGAAAG CTTACAAGCA 
TTATGCAGTT TTGCAGGTGA TATGGCAGCA GAAGTCATTA CAGAAGCTGA AAAAATAGCA 
AAAGTTAGGA GCTGTATGTT TTTCAGGAGG AAGAGGAACA GTTGTGTTGA TGGTGACCAA 
GATCGTAGAT CCGAAGAGAA GTTGGATGTA GAGGCTGTAG CGCACCCGGG AGGAGTAGAT 
CCGTTTGTTC TTCCTTTACC ACCAAGTTCT TGTCTGTCAG GTCTAACGTA CAAGTATCCC 
AGCTGTGAAA GTGTGACAGA TGAGTATGCA GGTCACATTA TCCAAATACT AAAACAGGAA 
GGTGGCAATA GTGAGTTAAT AATGGACCAG TACGCCAACA GACTTGCCTA TCGGTCTGTT 
AAGTCAGGAT TGCAAGAAGC AGCTAAAACA GCCAAAGTGA AGTGCAGCTC CAAAATACTC 
CCTGTGCAGA GCTCACAGGT GAAAACCAAC GATGAACTGT TACTGTTTTT AAATAAAGAA 
CACCAGCAAG AAGCAGATAA AAAAAGACAA AGCAGAAGTG CTGCAAGTTA CCTTCATAAA 
AACCGGACTT GTGAGTGGAC ACGGGACATG TACAGAAACG AGTGCTTGGA ACTACGTAGT 
TTTTCAACCT GTCTTGCTCA CAGCATCACA AGAGATGTTA AAAAAGAGTT GATGGCATCA 
ACAGGTGGCT TGCCAAAATC CTTAACAGAT TCTTGTCTTT ATGAAAAAAA CGTATGTGAC 
GAAGATACTG AGACTCATGT CGGGCCAGAG TTTCCCAAGT CTCTTCAGCC TTCTTCACAG 
AATCACAGAT TTTCTCAGAG TACAGGCAGC TTGAGTGGGT GTGGCTATGG AGAGACTGTC 
GTTCAAGCTA TAGAGCAGTA TGCTAACAAA GTCGTGGGCG ACACTTTAGA GCTGGGTTTA 
GGATCTGCCA TCTTCCACGT GTCTGAAGCC ACAAAAGCAG TTGATAGGAT TACTTACGCA 
GAAAAGCTGT CACCTCTGGT GGGTCAGGCT TGCCGATACT GTGACCGTAA AGAGCTCCAT 
GACTGCACTA GAAATTCGTC TCCTCACTTT CCCAGACCGG ATTCACTTGC TGGTAGTAAG 
CCAGTTTCTC ATTCACAGCT TAGCAGCTTC TATCAGAAAT CTAGGATTTT TCATCTTGAT 
GTTCCTCAAA TTCACGTTGA TCTCGATAAG AAGACAGTGC TTGCTGAGAA GATAGTTGCT 
GAAGCGATTG AAAAGGCAGA GAGAGAGCTG AGCAGTACCA GCCAGGCAGC CGACAGTGGG 
ATCGGACAGG AAGGTGTCAG CTTTGCCGAG AGCCTCAGCA CAGAGATACT GGCGTCAGCA 
GTGGTCAGTG CTGGACAGGC CATCAGCAGT CCAAAAGAAA CAGAAGAGAT TCAGTCAGCT 
GAGTCTCTTG GTAGCCAGCA GATGCATCTC AGTATTGGTG AGGACAGTAC TGGGAGCTGG 
TCCAACTTAA GTTTTGAAGA TGAACACCAG GATGAAAGCA GTAGTTTTCA CCATCTAAGT 
GAAAGTAATG GTAACAGCAG TAGCTGGAGC AGTCTTGGTT TAGAAGGAGA TTTGTATGAG 
GACAATTTAT CCTTTCCAAC ATCAGACAGT GATGGGCCAG ATGATGATGA AGAGGAGCGT 
CAGACTGATG TAGAAGGTTT GGAGCAAGAT GGAAAGACTC TGCTAATTAC AAATATTGAC 
ATGGAGCCAT GCACAGTAGA CCCCCAGCTA AGGATTATTC TTCAGTGGCT CGTTGCTTCT 
GAGGCAGAAG TTGCAGAACT TTATTTTCAT GACTCTACAA AGAAGGAGTT TATGCAACTT 
TCAAAACGGC TACAGGAAAA AGGGTGGAAG GTGGGAGACC TCCTGCAGGC TGTGCTTAAA 
TACTATGAGG AGGTGGAGAA GAAGTCCAGT GAGGAGAGAT GCAAGTCCCT GTTTGATTGG 
CTCTTGGAAA ATGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP11 ( XM_006076502.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006076564.1 Bubalus bubalis A kinase (PRKA) anchor protein 11 (AKAP11), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu30010
Clone ID Related Accession (Same CDS sequence)	XM_006076502.2 , XM_025263033.1 , XM_025263034.1 , XM_006076502.1
Accession Version	XM_006076502.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	A-kinase anchor protein 11 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785696.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCT TCCAGTCTTT CAGAAACAAT CACATGAAAA CTAGAGCATC TGTCAGAAAA 
AGCTTCAATG AAGATGTGTT CCAGTCTGTG AAGTCTTTAT TGCAGAGCGA GAAGGAACTA 
TGCAGTGTAT CAGCCGAGGA CTGTTTGAAG CAGGATGAAC ATGCCAATTT GACTGAGGTC 
ACATTTCTAG GTTTTAATGA AGAAACAGAT GCTGCTCATA TACAGGATTT GGCTGCAGTT 
TCTTTGGAAC TCCCAGATCT TCTGAACTCA CTGCACTTCT GCAGTCTAAA TGAAAATGAA 
ATTATTTGTA TGAAGGATAT AAATAAATCA TCAGATATAA ACAGTGATCC TCTAAACCAG 
AGTCAACATC CTGGAATGCT TTGTGTCATG AGAGTGTCAC CTACATTACC AAGACTTAAA 
ATTGATTTTA TTTTTAGTCT CCTAAGTAAA TATGCTACCG GCATAAGGTA CACCCTGGAC 
ACATATTTGC ATCACAAGCA CCAACTTGAG GCCACCAATG AAGACGACGA TGATACTAAC 
CAGTCTGTAT CTTCCATCGA AGATGACTTT GTCACTGCTT TTGAGCACTT AGACGAGGAA 
GAGACTTCAA AGCCATATAA TGATGGATTA AACATAATTG CACTAAGGAG CCAGTGTGAT 
GCTGCTTCAC AGACTATTTC TGGTCACCAT TTGGATACCC ATGATTTCAG GGTTCTGGTT 
GGCTCTGAGC AGCAGAAGTC ATTGGCTAAA CCTTCAGCTT CTTTAATAAA CATTCTGGGA 
CATAAAAAAC TGCCTCCAGC GAGAACTTCA GTCACGACAT CAGTCTCTGA ACCATGGATC 
CAGAGGAGTT TCTATAGTTC CTTTGCTGCT TCAGAGAAAG GTAGTGATGC CCAGAAACCA 
TTGTTCTCGT CTTCTCCTGC CTACTCGTCT GAATCAGAGT GCTCAAGTCC AAGTCCTGTT 
ATTTTCTTGG ATGAAGAAGG TTATCAAAAA AGTTTGAAAG CAAAACTTGA GTTACCTAAA 
ATTCCTGTGA TGAAAGATGA TATTGAGGAT TCAGACTCAG AAGTGAGTGA GTTTTTTGAT 
AGTTTTGATC AGTTTGATGA ACTAGAACAA ACGTTAGAGA CTGCTTGTCC GTTTCTGAAA 
GATCTTGCTG TAGGGAAGCC ATCGCAAAAG AAAGGGCACA AACATGAAAA ATTGTGCTCT 
GTAACCACTA CTATGAATCC TCAAAAATTC AAGTTTGATC GGCCAGCTCT CCCAGCTAAT 
GTTAGGAAGC CAACACCTCG TAAACCAGAA TCCCCTTATA GTAACCTGTG TGATGCCCCA 
GATTCTCCCC GCCCCGTGAA GGCCTCAGGG GAAGACAGTG GTTTGTTTAG TCCTATTCGA 
TCCTCTGCTT TCAGTCCTCT TGGAGGCTGT ACTCCAGCTG AATGCTTTTG CCAAACAGAT 
ATTGGTGGAG ATAGGGGTCA TGAAAATCCT GATTCTCTTT ATTATACCTA TGAAGATTAT 
GCAAATAGCC TTTCCTGTGA AGTTCTGGGC TCAGTTCTTC ATAGCCAACA TACTAATGCA 
ACATCCAACA CTAATAGTGT TAAAAAGGGA GAAAATAAAA TGGTAGCTTT TCAGCATGGA 
AGCCTTGATC AAAAAAGTAA ATCTAAAAAT AAACCCATAA TGATTGATAG CGTTCAGAAG 
TTTGCAGCAG ATCTCGTGGA AAAAAGTTTT GGCAGTGCAT TTAAAGACTT ACAGAAAGGG 
GTCTCTTCAT GTACCAATGC CTTATGCCAG TTAGCCATCA AATTGACGTC ATCCATTTTT 
CAAATGGCAT TTAACGAACT GAGAAGGCAG TGTGCCTTTT CACTGAAAGA ACGAGCCATT 
AGTGGCCTGG CTAGTTTTTT GGTGAGTGAG GCATTCTCAA ATGCTTTAAA AGATCTACAA 
TATGTAAAGA AACAGATCTT CACTAACACC GTCACTAGAT TTGCTGCAGA TCTTGCTGAA 
GAGCTTGTTT TTGAAGGCAT CATGGAAGTG TGTCAGTTCT CATATCCTCC AACACCTGCA 
TCTCTGCAGG GTCGGTCATT TCACTGTGAA GACAGAGTGG TGAAGTCCTA TGCAAGAGAT 
CTGTCTGAAT CTGTAATACA GGAAGCATTC ATTGAGCTGT CTCAAGTTGA TGTTACCTTT 
ACCACAAAGG CAGCAGTTAG TGTTTCCCCA GAAAACATCA AGTTTGTGAG CACAGAAGAT 
GTAGTGCCAG CAACACAGAC TTCTTTGTGT TCTTCTACTT CTAACAGTCA AACAGTTACG 
GTGACAAAAC CAGTGCAGGA GTATAAAAAG GAATACACGG TGCAGCAGGC CTTGTTCTGT 
ACTTCAGGAA TTGTTACTTC AATACCAGTG CCCTTGGCAG GAAGTGCCCT TCACCCATAC 
CGTATTTCTT CTAATACGTA TCAGACAAAG TCTCATCCAT CATGTGATGA TAGTCATTTG 
AATGGTGGTC CTACCCCAGC AAGTGTTGCC ACAAAAAACA AAGAAGAGGA GCTAGCTTGT 
CTCAGGAATA TTTGTTTACC TTCAGACCAC AACCCCGGTA GCCAGAATGA GGTGAAACCA 
ACTAGTGATG TTGTTGAAAC AAAAAGCTCT TCAAAGTTAA CAAATGATCC TGTCATTATT 
GGCGATTTTT CTGCCGCAAT GGTGCATACG ATAGTAAATG AAACTATAGA TTCACTGACA 
TCTTCCAAAG TTGCAAAAAC AGAAGAACAC ACAGATTGTT TAACTCAAAC AGGAAAGGGA 
AAAACCCCTT CTTTCTACTG GGATCAAGCA ACAGTGCCAC ACAGTGAGGC TAGAAAGAAG 
GACATGTTCG CTGATCGGTT ATCTAAATCT ATTATCAAAC ATTCCATAGA TGAAAGCAAG 
TCAGTGATCC TAAATGTAGA TAAAAATGTC CTCCCCAAGA AAGACTTGCC TGTTTCTGGA 
GATGAGTCAC AGTTGACCTT GGAAGAGTTC CCCAAGTTTC TTGAAGCTCA AGATCATTTA 
ACTCACTGTT CACTTTCAGA AGTAAAGGAT TGTGTTCCAG AAGGTAAAGG TTCTGTGGCT 
CCTGGATTTT CTTTGGAGAC GCTACCACCT TGTCTAGCAG CGGCAGGCCA GAAAGCTGAT 
CTGAAGGAAA TTGCTAAGGA CAAATCTGTG AAAAAGCAGA ATTTGCATAA TTCAGCCCTC 
GAGCCCTTGG CTTTTGGGCA GGAAAACTCT TTTCCCCATT CACATGCTTT CTCATCCACA 
GTGCTCACCT GTGTAGATGG TTTGCATGTG GAAGATAAAC AGAAAATGAG AGATGGGAAT 
GTAATACCTG ACACCCCTCC ATCAACTCCT CTGGTTCCAT CCCAGGCTCC TTCTGATTGG 
GATATCAAGA AGTTAACCAA AAAGCTTAAG GGGGAGTTAG CAAAAGAGTT TGCACCGGCC 
ACACCACCAT CCACACCTCA CAACTCATCT GTAAGCAGTT TGTCTGAAAA TGAACAAAAT 
GCAATAGAAA AAGAAGAGTT CATGTTGAAG CTCATGCGGT CTCTCTCAGA AGAAGTTGAA 
AGTAGTGACG GCGAAGAGCA GCAAGAGATG GACGTGAAAT TGGAGCCAGC AGGGAAAAAA 
GTTCAGTTTG CAGACGCATT AGCGACGCAC ATCGTTTCTC TTGCAACCGA AATGGCAGCT 
TCCCATTTGG ATAATAAATT GATTCAAGAA GCCGGGGTGA CAAGTCCTTG CTTAAATGTG 
CAGAGTCAAA GAAGCCTATC ACCTGCTTTT TTAAATTGCT CAGATGAAAG CTTACAAGCA 
TTATGCAGTT TTGCAGGTGA TATGGCAGCA GAAGTCATTA CAGAAGCTGA AAAAATAGCA 
AAAGTTAGGA GCTGTATGTT TTTCAGGAGG AAGAGGAACA GTTGTGTTGA TGGTGACCAA 
GATCGTAGAT CCGAAGAGAA GTTGGATGTA GAGGCTGTAG CGCACCCGGG AGGAGTAGAT 
CCGTTTGTTC TTCCTTTACC ACCAAGTTCT TGTCTGTCAG GTCTAACGTA CAAGTATCCC 
AGCTGTGAAA GTGTGACAGA TGAGTATGCA GGTCACATTA TCCAAATACT AAAACAGGAA 
GGTGGCAATA GTGAGTTAAT AATGGACCAG TACGCCAACA GACTTGCCTA TCGGTCTGTT 
AAGTCAGGAT TGCAAGAAGC AGCTAAAACA GCCAAAGTGA AGTGCAGCTC CAAAATACTC 
CCTGTGCAGA GCTCACAGGT GAAAACCAAC GATGAACTGT TACTGTTTTT AAATAAAGAA 
CACCAGCAAG AAGCAGATAA AAAAAGACAA AGCAGAAGTG CTGCAAGTTA CCTTCATAAA 
AACCGGACTT GTGAGTGGAC ACGGGACATG TACAGAAACG AGTGCTTGGA ACTACGTAGT 
TTTTCAACCT GTCTTGCTCA CAGCATCACA AGAGATGTTA AAAAAGAGTT GATGGCATCA 
ACAGGTGGCT TGCCAAAATC CTTAACAGAT TCTTGTCTTT ATGAAAAAAA CGTATGTGAC 
GAAGATACTG AGACTCATGT CGGGCCAGAG TTTCCCAAGT CTCTTCAGCC TTCTTCACAG 
AATCACAGAT TTTCTCAGAG TACAGGCAGC TTGAGTGGGT GTGGCTATGG AGAGACTGTC 
GTTCAAGCTA TAGAGCAGTA TGCTAACAAA GTCGTGGGCG ACACTTTAGA GCTGGGTTTA 
GGATCTGCCA TCTTCCACGT GTCTGAAGCC ACAAAAGCAG TTGATAGGAT TACTTACGCA 
GAAAAGCTGT CACCTCTGGT GGGTCAGGCT TGCCGATACT GTGACCGTAA AGAGCTCCAT 
GACTGCACTA GAAATTCGTC TCCTCACTTT CCCAGACCGG ATTCACTTGC TGGTAGTAAG 
CCAGTTTCTC ATTCACAGCT TAGCAGCTTC TATCAGAAAT CTAGGATTTT TCATCTTGAT 
GTTCCTCAAA TTCACGTTGA TCTCGATAAG AAGACAGTGC TTGCTGAGAA GATAGTTGCT 
GAAGCGATTG AAAAGGCAGA GAGAGAGCTG AGCAGTACCA GCCAGGCAGC CGACAGTGGG 
ATCGGACAGG AAGGTGTCAG CTTTGCCGAG AGCCTCAGCA CAGAGATACT GGCGTCAGCA 
GTGGTCAGTG CTGGACAGGC CATCAGCAGT CCAAAAGAAA CAGAAGAGAT TCAGTCAGCT 
GAGTCTCTTG GTAGCCAGCA GATGCATCTC AGTATTGGTG AGGACAGTAC TGGGAGCTGG 
TCCAACTTAA GTTTTGAAGA TGAACACCAG GATGAAAGCA GTAGTTTTCA CCATCTAAGT 
GAAAGTAATG GTAACAGCAG TAGCTGGAGC AGTCTTGGTT TAGAAGGAGA TTTGTATGAG 
GACAATTTAT CCTTTCCAAC ATCAGACAGT GATGGGCCAG ATGATGATGA AGAGGAGCGT 
CAGACTGATG TAGAAGGTTT GGAGCAAGAT GGAAAGACTC TGCTAATTAC AAATATTGAC 
ATGGAGCCAT GCACAGTAGA CCCCCAGCTA AGGATTATTC TTCAGTGGCT CGTTGCTTCT 
GAGGCAGAAG TTGCAGAACT TTATTTTCAT GACTCTACAA AGAAGGAGTT TATGCAACTT 
TCAAAACGGC TACAGGAAAA AGGGTGGAAG GTGGGAGACC TCCTGCAGGC TGTGCTTAAA 
TACTATGAGG AGGTGGAGAA GAAGTCCAGT GAGGAGAGAT GCAAGTCCCT GTTTGATTGG 
CTCTTGGAAA ATGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006076564.1
CDS	96..5813
Translation	MATFQSFRNNHMKTRASVRKSFNEDVFQSVKSLLQSEKELCSVSAEDCLKQDEHANLTEVTFLGFNEETDAAHIQDLAAVSLELPDLLNSLHFCSLNENEIICMKDINKSSDINSDPLNQSQHPGMLCVMRVSPTLPRLKIDFIFSLLSKYATGIRYTLDTYLHHKHQLEATNEDDDDTNQSVSSIEDDFVTAFEHLDEEETSKPYNDGLNIIALRSQCDAASQTISGHHLDTHDFRVLVGSEQQKSLAKPSASLINILGHKKLPPARTSVTTSVSEPWIQRSFYSSFAASEKGSDAQKPLFSSSPAYSSESECSSPSPVIFLDEEGYQKSLKAKLELPKIPVMKDDIEDSDSEVSEFFDSFDQFDELEQTLETACPFLKDLAVGKPSQKKGHKHEKLCSVTTTMNPQKFKFDRPALPANVRKPTPRKPESPYSNLCDAPDSPRPVKASGEDSGLFSPIRSSAFSPLGGCTPAECFCQTDIGGDRGHENPDSLYYTYEDYANSLSCEVLGSVLHSQHTNATSNTNSVKKGENKMVAFQHGSLDQKSKSKNKPIMIDSVQKFAADLVEKSFGSAFKDLQKGVSSCTNALCQLAIKLTSSIFQMAFNELRRQCAFSLKERAISGLASFLVSEAFSNALKDLQYVKKQIFTNTVTRFAADLAEELVFEGIMEVCQFSYPPTPASLQGRSFHCEDRVVKSYARDLSESVIQEAFIELSQVDVTFTTKAAVSVSPENIKFVSTEDVVPATQTSLCSSTSNSQTVTVTKPVQEYKKEYTVQQALFCTSGIVTSIPVPLAGSALHPYRISSNTYQTKSHPSCDDSHLNGGPTPASVATKNKEEELACLRNICLPSDHNPGSQNEVKPTSDVVETKSSSKLTNDPVIIGDFSAAMVHTIVNETIDSLTSSKVAKTEEHTDCLTQTGKGKTPSFYWDQATVPHSEARKKDMFADRLSKSIIKHSIDESKSVILNVDKNVLPKKDLPVSGDESQLTLEEFPKFLEAQDHLTHCSLSEVKDCVPEGKGSVAPGFSLETLPPCLAAAGQKADLKEIAKDKSVKKQNLHNSALEPLAFGQENSFPHSHAFSSTVLTCVDGLHVEDKQKMRDGNVIPDTPPSTPLVPSQAPSDWDIKKLTKKLKGELAKEFAPATPPSTPHNSSVSSLSENEQNAIEKEEFMLKLMRSLSEEVESSDGEEQQEMDVKLEPAGKKVQFADALATHIVSLATEMAASHLDNKLIQEAGVTSPCLNVQSQRSLSPAFLNCSDESLQALCSFAGDMAAEVITEAEKIAKVRSCMFFRRKRNSCVDGDQDRRSEEKLDVEAVAHPGGVDPFVLPLPPSSCLSGLTYKYPSCESVTDEYAGHIIQILKQEGGNSELIMDQYANRLAYRSVKSGLQEAAKTAKVKCSSKILPVQSSQVKTNDELLLFLNKEHQQEADKKRQSRSAASYLHKNRTCEWTRDMYRNECLELRSFSTCLAHSITRDVKKELMASTGGLPKSLTDSCLYEKNVCDEDTETHVGPEFPKSLQPSSQNHRFSQSTGSLSGCGYGETVVQAIEQYANKVVGDTLELGLGSAIFHVSEATKAVDRITYAEKLSPLVGQACRYCDRKELHDCTRNSSPHFPRPDSLAGSKPVSHSQLSSFYQKSRIFHLDVPQIHVDLDKKTVLAEKIVAEAIEKAERELSSTSQAADSGIGQEGVSFAESLSTEILASAVVSAGQAISSPKETEEIQSAESLGSQQMHLSIGEDSTGSWSNLSFEDEHQDESSSFHHLSESNGNSSSWSSLGLEGDLYEDNLSFPTSDSDGPDDDEEERQTDVEGLEQDGKTLLITNIDMEPCTVDPQLRIILQWLVASEAEVAELYFHDSTKKEFMQLSKRLQEKGWKVGDLLQAVLKYYEEVEKKSSEERCKSLFDWLLENA

Target ORF information:

RefSeq Version	XM_006076502.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis A kinase (PRKA) anchor protein 11 (AKAP11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006076502.1

ORF Insert Sequence:

ATGGCGACCT TCCAGTCTTT CAGAAACAAT CACATGAAAA CTAGAGCATC TGTCAGAAAA 
AGCTTCAATG AAGATGTGTT CCAGTCTGTG AAGTCTTTAT TGCAGAGCGA GAAGGAACTA 
TGCAGTGTAT CAGCCGAGGA CTGTTTGAAG CAGGATGAAC ATGCCAATTT GACTGAGGTC 
ACATTTCTAG GTTTTAATGA AGAAACAGAT GCTGCTCATA TACAGGATTT GGCTGCAGTT 
TCTTTGGAAC TCCCAGATCT TCTGAACTCA CTGCACTTCT GCAGTCTAAA TGAAAATGAA 
ATTATTTGTA TGAAGGATAT AAATAAATCA TCAGATATAA ACAGTGATCC TCTAAACCAG 
AGTCAACATC CTGGAATGCT TTGTGTCATG AGAGTGTCAC CTACATTACC AAGACTTAAA 
ATTGATTTTA TTTTTAGTCT CCTAAGTAAA TATGCTACCG GCATAAGGTA CACCCTGGAC 
ACATATTTGC ATCACAAGCA CCAACTTGAG GCCACCAATG AAGACGACGA TGATACTAAC 
CAGTCTGTAT CTTCCATCGA AGATGACTTT GTCACTGCTT TTGAGCACTT AGACGAGGAA 
GAGACTTCAA AGCCATATAA TGATGGATTA AACATAATTG CACTAAGGAG CCAGTGTGAT 
GCTGCTTCAC AGACTATTTC TGGTCACCAT TTGGATACCC ATGATTTCAG GGTTCTGGTT 
GGCTCTGAGC AGCAGAAGTC ATTGGCTAAA CCTTCAGCTT CTTTAATAAA CATTCTGGGA 
CATAAAAAAC TGCCTCCAGC GAGAACTTCA GTCACGACAT CAGTCTCTGA ACCATGGATC 
CAGAGGAGTT TCTATAGTTC CTTTGCTGCT TCAGAGAAAG GTAGTGATGC CCAGAAACCA 
TTGTTCTCGT CTTCTCCTGC CTACTCGTCT GAATCAGAGT GCTCAAGTCC AAGTCCTGTT 
ATTTTCTTGG ATGAAGAAGG TTATCAAAAA AGTTTGAAAG CAAAACTTGA GTTACCTAAA 
ATTCCTGTGA TGAAAGATGA TATTGAGGAT TCAGACTCAG AAGTGAGTGA GTTTTTTGAT 
AGTTTTGATC AGTTTGATGA ACTAGAACAA ACGTTAGAGA CTGCTTGTCC GTTTCTGAAA 
GATCTTGCTG TAGGGAAGCC ATCGCAAAAG AAAGGGCACA AACATGAAAA ATTGTGCTCT 
GTAACCACTA CTATGAATCC TCAAAAATTC AAGTTTGATC GGCCAGCTCT CCCAGCTAAT 
GTTAGGAAGC CAACACCTCG TAAACCAGAA TCCCCTTATA GTAACCTGTG TGATGCCCCA 
GATTCTCCCC GCCCCGTGAA GGCCTCAGGG GAAGACAGTG GTTTGTTTAG TCCTATTCGA 
TCCTCTGCTT TCAGTCCTCT TGGAGGCTGT ACTCCAGCTG AATGCTTTTG CCAAACAGAT 
ATTGGTGGAG ATAGGGGTCA TGAAAATCCT GATTCTCTTT ATTATACCTA TGAAGATTAT 
GCAAATAGCC TTTCCTGTGA AGTTCTGGGC TCAGTTCTTC ATAGCCAACA TACTAATGCA 
ACATCCAACA CTAATAGTGT TAAAAAGGGA GAAAATAAAA TGGTAGCTTT TCAGCATGGA 
AGCCTTGATC AAAAAAGTAA ATCTAAAAAT AAACCCATAA TGATTGATAG CGTTCAGAAG 
TTTGCAGCAG ATCTCGTGGA AAAAAGTTTT GGCAGTGCAT TTAAAGACTT ACAGAAAGGG 
GTCTCTTCAT GTACCAATGC CTTATGCCAG TTAGCCATCA AATTGACGTC ATCCATTTTT 
CAAATGGCAT TTAACGAACT GAGAAGGCAG TGTGCCTTTT CACTGAAAGA ACGAGCCATT 
AGTGGCCTGG CTAGTTTTTT GGTGAGTGAG GCATTCTCAA ATGCTTTAAA AGATCTACAA 
TATGTAAAGA AACAGATCTT CACTAACACC GTCACTAGAT TTGCTGCAGA TCTTGCTGAA 
GAGCTTGTTT TTGAAGGCAT CATGGAAGTG TGTCAGTTCT CATATCCTCC AACACCTGCA 
TCTCTGCAGG GTCGGTCATT TCACTGTGAA GACAGAGTGG TGAAGTCCTA TGCAAGAGAT 
CTGTCTGAAT CTGTAATACA GGAAGCATTC ATTGAGCTGT CTCAAGTTGA TGTTACCTTT 
ACCACAAAGG CAGCAGTTAG TGTTTCCCCA GAAAACATCA AGTTTGTGAG CACAGAAGAT 
GTAGTGCCAG CAACACAGAC TTCTTTGTGT TCTTCTACTT CTAACAGTCA AACAGTTACG 
GTGACAAAAC CAGTGCAGGA GTATAAAAAG GAATACACGG TGCAGCAGGC CTTGTTCTGT 
ACTTCAGGAA TTGTTACTTC AATACCAGTG CCCTTGGCAG GAAGTGCCCT TCACCCATAC 
CGTATTTCTT CTAATACGTA TCAGACAAAG TCTCATCCAT CATGTGATGA TAGTCATTTG 
AATGGTGGTC CTACCCCAGC AAGTGTTGCC ACAAAAAACA AAGAAGAGGA GCTAGCTTGT 
CTCAGGAATA TTTGTTTACC TTCAGACCAC AACCCCGGTA GCCAGAATGA GGTGAAACCA 
ACTAGTGATG TTGTTGAAAC AAAAAGCTCT TCAAAGTTAA CAAATGATCC TGTCATTATT 
GGCGATTTTT CTGCCGCAAT GGTGCATACG ATAGTAAATG AAACTATAGA TTCACTGACA 
TCTTCCAAAG TTGCAAAAAC AGAAGAACAC ACAGATTGTT TAACTCAAAC AGGAAAGGGA 
AAAACCCCTT CTTTCTACTG GGATCAAGCA ACAGTGCCAC ACAGTGAGGC TAGAAAGAAG 
GACATGTTCG CTGATCGGTT ATCTAAATCT ATTATCAAAC ATTCCATAGA TGAAAGCAAG 
TCAGTGATCC TAAATGTAGA TAAAAATGTC CTCCCCAAGA AAGACTTGCC TGTTTCTGGA 
GATGAGTCAC AGTTGACCTT GGAAGAGTTC CCCAAGTTTC TTGAAGCTCA AGATCATTTA 
ACTCACTGTT CACTTTCAGA AGTAAAGGAT TGTGTTCCAG AAGGTAAAGG TTCTGTGGCT 
CCTGGATTTT CTTTGGAGAC GCTACCACCT TGTCTAGCAG CGGCAGGCCA GAAAGCTGAT 
CTGAAGGAAA TTGCTAAGGA CAAATCTGTG AAAAAGCAGA ATTTGCATAA TTCAGCCCTC 
GAGCCCTTGG CTTTTGGGCA GGAAAACTCT TTTCCCCATT CACATGCTTT CTCATCCACA 
GTGCTCACCT GTGTAGATGG TTTGCATGTG GAAGATAAAC AGAAAATGAG AGATGGGAAT 
GTAATACCTG ACACCCCTCC ATCAACTCCT CTGGTTCCAT CCCAGGCTCC TTCTGATTGG 
GATATCAAGA AGTTAACCAA AAAGCTTAAG GGGGAGTTAG CAAAAGAGTT TGCACCGGCC 
ACACCACCAT CCACACCTCA CAACTCATCT GTAAGCAGTT TGTCTGAAAA TGAACAAAAT 
GCAATAGAAA AAGAAGAGTT CATGTTGAAG CTCATGCGGT CTCTCTCAGA AGAAGTTGAA 
AGTAGTGACG GCGAAGAGCA GCAAGAGATG GACGTGAAAT TGGAGCCAGC AGGGAAAAAA 
GTTCAGTTTG CAGACGCATT AGCGACGCAC ATCGTTTCTC TTGCAACCGA AATGGCAGCT 
TCCCATTTGG ATAATAAATT GATTCAAGAA GCCGGGGTGA CAAGTCCTTG CTTAAATGTG 
CAGAGTCAAA GAAGCCTATC ACCTGCTTTT TTAAATTGCT CAGATGAAAG CTTACAAGCA 
TTATGCAGTT TTGCAGGTGA TATGGCAGCA GAAGTCATTA CAGAAGCTGA AAAAATAGCA 
AAAGTTAGGA GCTGTATGTT TTTCAGGAGG AAGAGGAACA GTTGTGTTGA TGGTGACCAA 
GATCGTAGAT CCGAAGAGAA GTTGGATGTA GAGGCTGTAG CGCACCCGGG AGGAGTAGAT 
CCGTTTGTTC TTCCTTTACC ACCAAGTTCT TGTCTGTCAG GTCTAACGTA CAAGTATCCC 
AGCTGTGAAA GTGTGACAGA TGAGTATGCA GGTCACATTA TCCAAATACT AAAACAGGAA 
GGTGGCAATA GTGAGTTAAT AATGGACCAG TACGCCAACA GACTTGCCTA TCGGTCTGTT 
AAGTCAGGAT TGCAAGAAGC AGCTAAAACA GCCAAAGTGA AGTGCAGCTC CAAAATACTC 
CCTGTGCAGA GCTCACAGGT GAAAACCAAC GATGAACTGT TACTGTTTTT AAATAAAGAA 
CACCAGCAAG AAGCAGATAA AAAAAGACAA AGCAGAAGTG CTGCAAGTTA CCTTCATAAA 
AACCGGACTT GTGAGTGGAC ACGGGACATG TACAGAAACG AGTGCTTGGA ACTACGTAGT 
TTTTCAACCT GTCTTGCTCA CAGCATCACA AGAGATGTTA AAAAAGAGTT GATGGCATCA 
ACAGGTGGCT TGCCAAAATC CTTAACAGAT TCTTGTCTTT ATGAAAAAAA CGTATGTGAC 
GAAGATACTG AGACTCATGT CGGGCCAGAG TTTCCCAAGT CTCTTCAGCC TTCTTCACAG 
AATCACAGAT TTTCTCAGAG TACAGGCAGC TTGAGTGGGT GTGGCTATGG AGAGACTGTC 
GTTCAAGCTA TAGAGCAGTA TGCTAACAAA GTCGTGGGCG ACACTTTAGA GCTGGGTTTA 
GGATCTGCCA TCTTCCACGT GTCTGAAGCC ACAAAAGCAG TTGATAGGAT TACTTACGCA 
GAAAAGCTGT CACCTCTGGT GGGTCAGGCT TGCCGATACT GTGACCGTAA AGAGCTCCAT 
GACTGCACTA GAAATTCGTC TCCTCACTTT CCCAGACCGG ATTCACTTGC TGGTAGTAAG 
CCAGTTTCTC ATTCACAGCT TAGCAGCTTC TATCAGAAAT CTAGGATTTT TCATCTTGAT 
GTTCCTCAAA TTCACGTTGA TCTCGATAAG AAGACAGTGC TTGCTGAGAA GATAGTTGCT 
GAAGCGATTG AAAAGGCAGA GAGAGAGCTG AGCAGTACCA GCCAGGCAGC CGACAGTGGG 
ATCGGACAGG AAGGTGTCAG CTTTGCCGAG AGCCTCAGCA CAGAGATACT GGCGTCAGCA 
GTGGTCAGTG CTGGACAGGC CATCAGCAGT CCAAAAGAAA CAGAAGAGAT TCAGTCAGCT 
GAGTCTCTTG GTAGCCAGCA GATGCATCTC AGTATTGGTG AGGACAGTAC TGGGAGCTGG 
TCCAACTTAA GTTTTGAAGA TGAACACCAG GATGAAAGCA GTAGTTTTCA CCATCTAAGT 
GAAAGTAATG GTAACAGCAG TAGCTGGAGC AGTCTTGGTT TAGAAGGAGA TTTGTATGAG 
GACAATTTAT CCTTTCCAAC ATCAGACAGT GATGGGCCAG ATGATGATGA AGAGGAGCGT 
CAGACTGATG TAGAAGGTTT GGAGCAAGAT GGAAAGACTC TGCTAATTAC AAATATTGAC 
ATGGAGCCAT GCACAGTAGA CCCCCAGCTA AGGATTATTC TTCAGTGGCT CGTTGCTTCT 
GAGGCAGAAG TTGCAGAACT TTATTTTCAT GACTCTACAA AGAAGGAGTT TATGCAACTT 
TCAAAACGGC TACAGGAAAA AGGGTGGAAG GTGGGAGACC TCCTGCAGGC TGTGCTTAAA 
TACTATGAGG AGGTGGAGAA GAAGTCCAGT GAGGAGAGAT GCAAGTCCCT GTTTGATTGG 
CTCTTGGAAA ATGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP11 ( XM_006076503.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006076565.1 Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu30011
Clone ID Related Accession (Same CDS sequence)	XM_006076503.2 , XM_006076503.1
Accession Version	XM_006076503.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	A-kinase anchor protein 11 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006076503.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCT TCCAGTCTTT CAGAAACAAT CACATGAAAA CTAGAGCATC TGTCAGAAAA 
AGCTTCAATG AAGATGTGTT CCAGTCTGTG AAGTCTTTAT TGCAGAGCGA GAAGGAACTA 
TGCAGTGTAT CAGCCGAGGA CTGTTTGAAG CAGGATGAAC ATGCCAATTT GACTGAGGTC 
ACATTTCTAG GTTTTAATGA AGAAACAGAT GCTGCTCATA TACAGGATTT GGCTGCAGTT 
TCTTTGGAAC TCCCAGATCT TCTGAACTCA CTGCACTTCT GCAGTCTAAA TGAAAATGAA 
ATTATTTGTA TGAAGGATAT AAATAAATCA TCAGATATAA ACAGTGATCC TCTAAACCAG 
AGTCAACATC CTGGAATGCT TTGTGTCATG AGAGTGTCAC CTACATTACC AAGACTTAAA 
ATTGATTTTA TTTTTAGTCT CCTAAGTAAA TATGCTACCG GCATAAGGTA CACCCTGGAC 
ACATATTTGC ATCACAAGCA CCAACTTGAG GCCACCAATG AAGACGACGA TGATACTAAC 
CAGTCTGTAT CTTCCATCGA AGATGACTTT GTCACTGCTT TTGAGCACTT AGACGAGGAA 
GAGACTTCAA AGCCATATAA TGATGGATTA AACATAATTG CACTAAGGAG CCAGTGTGAT 
GCTGCTTCAC AGACTATTTC TGGTCACCAT TTGGATACCC ATGATTTCAG GGTTCTGGTT 
GGCTCTGAGC AGCAGAAGTC ATTGGCTAAA CCTTCAGCTT CTTTAATAAA CATTCTGGGA 
CATAAAAAAC TGCCTCCAGC GAGAACTTCA GTCACGACAT CAGTCTCTGA ACCATGGATC 
CAGAGGAGTT TCTATAGTTC CTTTGCTGCT TCAGAGAAAG GTAGTGATGC CCAGAAACCA 
TTGTTCTCGT CTTCTCCTGC CTACTCGTCT GAATCAGAGT GCTCAAGTCC AAGTCCTGTT 
ATTTTCTTGG ATGAAGAAGG TTATCAAAAA AGTTTGAAAG CAAAACTTGA GTTACCTAAA 
ATTCCTGTGA TGAAAGATGA TATTGAGGAT TCAGACTCAG AAGTGAGTGA GTTTTTTGAT 
AGTTTTGATC AGTTTGATGA ACTAGAACAA ACGTTAGAGA CTGCTTGTCC GTTTCTGAAA 
GATCTTGCTG TAGGGAAGCC ATCGCAAAAG AAAGGGCACA AACATGAAAA ATTGTGCTCT 
GTAACCACTA CTATGAATCC TCAAAAATTC AAGTTTGATC GGCCAGCTCT CCCAGCTAAT 
GTTAGGAAGC CAACACCTCG TAAACCAGAA TCCCCTTATA GTAACCTGTG TGATGCCCCA 
GATTCTCCCC GCCCCGTGAA GGCCTCAGGG GAAGACAGTG GTTTGTTTAG TCCTATTCGA 
TCCTCTGCTT TCAGTCCTCT TGGAGGCTGT ACTCCAGCTG AATGCTTTTG CCAAACAGAT 
ATTGGTGGAG ATAGGGGTCA TGAAAATCCT GATTCTCTTT ATTATACCTA TGAAGATTAT 
GCAAATAGCC TTTCCTGTGA AGTTCTGGGC TCAGTTCTTC ATAGCCAACA TACTAATGCA 
ACATCCAACA CTAATAGTGT TAAAAAGGGA GAAAATAAAA TGGTAGCTTT TCAGCATGGA 
AGCCTTGATC AAAAAAGTAA ATCTAAAAAT AAACCCATAA TGATTGATAG CGTTCAGAAG 
TTTGCAGCAG ATCTCGTGGA AAAAAGTTTT GGCAGTGCAT TTAAAGACTT ACAGAAAGGG 
GTCTCTTCAT GTACCAATGC CTTATGCCAG TTAGCCATCA AATTGACGTC ATCCATTTTT 
CAAATGGCAT TTAACGAACT GAGAAGGCAG TGTGCCTTTT CACTGAAAGA ACGAGCCATT 
AGTGGCCTGG CTAGTTTTTT GGTGAGTGAG GCATTCTCAA ATGCTTTAAA AGATCTACAA 
TATGTAAAGA AACAGATCTT CACTAACACC GTCACTAGAT TTGCTGCAGA TCTTGCTGAA 
GAGCTTGTTT TTGAAGGCAT CATGGAAGTG TGTCAGTTCT CATATCCTCC AACACCTGCA 
TCTCTGCAGG GTCGGTCATT TCACTGTGAA GACAGAGTGG TGAAGTCCTA TGCAAGAGAT 
CTGTCTGAAT CTGTAATACA GGAAGCATTC ATTGAGCTGT CTCAAGTTGA TGTTACCTTT 
ACCACAAAGG CAGCAGTTAG TGTTTCCCCA GAAAACATCA AGTTTGTGAG CACAGAAGAT 
GTAGTGCCAG CAACACAGAC TTCTTTGTGT TCTTCTACTT CTAACAGTCA AACAGTTACG 
GTGACAAAAC CAGTGCAGGA GTATAAAAAG GAATACACGG TGCAGCAGGC CTTGTTCTGT 
ACTTCAGGAA TTGTTACTTC AATACCAGTG CCCTTGGCAG GAAGTGCCCT TCACCCATAC 
CGTATTTCTT CTAATACGTA TCAGACAAAG TCTCATCCAT CATGTGATGA TAGTCATTTG 
AATGGTGGTC CTACCCCAGC AAGTGTTGCC ACAAAAAACA AAGAAGAGGA GCTAGCTTGT 
CTCAGGAATA TTTGTTTACC TTCAGACCAC AACCCCGGTA GCCAGAATGA GGTGAAACCA 
ACTAGTGATG TTGTTGAAAC AAAAAGCTCT TCAAAGTTAA CAAATGATCC TGTCATTATT 
GGCGATTTTT CTGCCGCAAT GGTGCATACG ATAGTAAATG AAACTATAGA TTCACTGACA 
TCTTCCAAAG TTGCAAAAAC AGAAGAACAC ACAGATTGTT TAACTCAAAC AGGAAAGGGA 
AAAACCCCTT CTTTCTACTG GGATCAAGCA ACAGTGCCAC ACAGTGAGGC TAGAAAGAAG 
GACATGTTCG CTGATCGGTT ATCTAAATCT ATTATCAAAC ATTCCATAGA TGAAAGCAAG 
TCAGTGATCC TAAATGTAGA TAAAAATGTC CTCCCCAAGA AAGACTTGCC TGTTTCTGGA 
GATGAGTCAC AGTTGACCTT GGAAGAGTTC CCCAAGTTTC TTGAAGCTCA AGATCATTTA 
ACTCACTGTT CACTTTCAGA AGTAAAGGAT TGTGTTCCAG AAGGTAAAGG TTCTGTGGCT 
CCTGGATTTT CTTTGGAGAC GCTACCACCT TGTCTAGCAG CGGCAGGCCA GAAAGCTGAT 
CTGAAGGAAA TTGCTAAGGA CAAATCTGTG AAAAAGCAGA ATTTGCATAA TTCAGCCCTC 
GAGCCCTTGG CTTTTGGGCA GGAAAACTCT TTTCCCCATT CACATGCTTT CTCATCCACA 
GTGCTCACCT GTGTAGATGG TTTGCATGTG GAAGATAAAC AGAAAATGAG AGATGGGAAT 
GTAATACCTG ACACCCCTCC ATCAACTCCT CTGGTTCCAT CCCAGGCTCC TTCTGATTGG 
GATATCAAGA AGTTAACCAA AAAGCTTAAG GGGGAGTTAG CAAAAGAGTT TGCACCGGCC 
ACACCACCAT CCACACCTCA CAACTCATCT GTAAGCAGTT TGTCTGAAAA TGAACAAAAT 
GCAATAGAAA AAGAAGAGTT CATGTTGAAG CTCATGCGGT CTCTCTCAGA AGAAGTTGAA 
AGTAGTGACG GCGAAGAGCA GCAAGAGATG GACGTGAAAT TGGAGCCAGC AGGGAAAAAA 
GTTCAGTTTG CAGACGCATT AGCGACGCAC ATCGTTTCTC TTGCAACCGA AATGGCAGCT 
TCCCATTTGG ATAATAAATT GATTCAAGAA GCCGGGGTGA CAAGTCCTTG CTTAAATGTG 
CAGAGTCAAA GAAGCCTATC ACCTGCTTTT TTAAATTGCT CAGATGAAAG CTTACAAGCA 
TTATGCAGTT TTGCAGGTGA TATGGCAGCA GAAGTCATTA CAGAAGCTGA AAAAATAGCA 
AAAGTTAGGA GCTGTATGTT TTTCAGGAGG AAGAGGAACA GTTGTGTTGA TGGTGACCAA 
GATCGTAGAT CCGAAGAGAA GTTGGATGTA GAGGCTGTAG CGCACCCGGG AGGAGTAGAT 
CCGTTTGTTC TTCCTTTACC ACCAAGTTCT TGTCTGTCAG GTCTAACGTA CAAGTATCCC 
AGCTGTGAAA GTGTGACAGA TGAGTATGCA GGTCACATTA TCCAAATACT AAAACAGGAA 
GGTGGCAATA GTGAGTTAAT AATGGACCAG TACGCCAACA GACTTGCCTA TCGGTCTGTT 
AAGTCAGGAT TGCAAGAAGC AGCTAAAACA GCCAAAGTGA AGTGCAGCTC CAAAATACTC 
CCTGTGCAGA GCTCACAGGT GAAAACCAAC GATGAACTGT TACTGTTTTT AAATAAAGAA 
CACCAGCAAG AAGCAGATAA AAAAAGACAA AGCAGAAGTG CTGCAAGTTA CCTTCATAAA 
AACCGGACTT GTGAGTGGAC ACGGGACATG TACAGAAACG AGTGCTTGGA ACTACGTAGT 
TTTTCAACCT GTCTTGCTCA CAGCATCACA AGAGATGTTA AAAAAGAGTT GATGGCATCA 
ACAGGTGGCT TGCCAAAATC CTTAACAGAT TCTTGTCTTT ATGAAAAAAA CGTATGTGAC 
GAAGATACTG AGACTCATGT CGGGCCAGAG TTTCCCAAGT CTCTTCAGCC TTCTTCACAG 
AATCACAGAT TTTCTCAGAG TACAGGCAGC TTGAGTGGGT GTGGCTATGG AGAGACTGTC 
GTTCAAGCTA TAGAGCAGTA TGCTAACAAA GTCGTGGGCG ACACTTTAGA GCTGGGTTTA 
GGATCTGCCA TCTTCCACGT GTCTGAAGCC ACAAAAGCAG TTGATAGGAT TACTTACGCA 
GAAAAGCTGT CACCTCTGGT GGGTCAGGCT TGCCGATACT GTGACCGTAA AGAGCTCCAT 
GACTGCACTA GAAATTCGTC TCCTCACTTT CCCAGACCGG ATTCACTTGC TGGTAGTAAG 
CCAGTTTCTC ATTCACAGCT TAGCAGCTTC TATCAGAAAT CTAGGATTTT TCATCTTGAT 
GTTCCTCAAA TTCACGTTGA TCTCGATAAG AAGACAGTGC TTGCTGAGAA GATAGTTGCT 
GAAGCGATTG AAAAGGCAGA GAGAGAGCTG AGCAGTACCA GCCAGGCAGC CGACAGTGGG 
ATCGGACAGG AAGGTGTCAG CTTTGCCGAG AGCCTCAGCA CAGAGATACT GGCGTCAGCA 
GTGGTCAGTG CTGGACAGGC CATCAGCAGT CCAAAAGAAA CAGAAGAGAT TCAGTCAGCT 
GAGTCTCTTG GTAGCCAGCA GATGCATCTC AGTATTGGTG AGGACAGTAC TGGGAGCTGG 
TCCAACTTAA GTTTTGAAGA TGAACACCAG GATGAAAGCA GTAGTTTTCA CCATCTAAGT 
GAAAGTGATG GGCCAGATGA TGATGAAGAG GAGCGTCAGA CTGATGTAGA AGGTTTGGAG 
CAAGATGGAA AGACTCTGCT AATTACAAAT ATTGACATGG AGCCATGCAC AGTAGACCCC 
CAGCTAAGGA TTATTCTTCA GTGGCTCGTT GCTTCTGAGG CAGAAGTTGC AGAACTTTAT 
TTTCATGACT CTACAAAGAA GGAGTTTATG CAACTTTCAA AACGGCTACA GGAAAAAGGG 
TGGAAGGTGG GAGACCTCCT GCAGGCTGTG CTTAAATACT ATGAGGAGGT GGAGAAGAAG 
TCCAGTGAGG AGAGATGCAA GTCCCTGTTT GATTGGCTCT TGGAAAATGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006076565.1
CDS	178..5811
Translation	MATFQSFRNNHMKTRASVRKSFNEDVFQSVKSLLQSEKELCSVSAEDCLKQDEHANLTEVTFLGFNEETDAAHIQDLAAVSLELPDLLNSLHFCSLNENEIICMKDINKSSDINSDPLNQSQHPGMLCVMRVSPTLPRLKIDFIFSLLSKYATGIRYTLDTYLHHKHQLEATNEDDDDTNQSVSSIEDDFVTAFEHLDEEETSKPYNDGLNIIALRSQCDAASQTISGHHLDTHDFRVLVGSEQQKSLAKPSASLINILGHKKLPPARTSVTTSVSEPWIQRSFYSSFAASEKGSDAQKPLFSSSPAYSSESECSSPSPVIFLDEEGYQKSLKAKLELPKIPVMKDDIEDSDSEVSEFFDSFDQFDELEQTLETACPFLKDLAVGKPSQKKGHKHEKLCSVTTTMNPQKFKFDRPALPANVRKPTPRKPESPYSNLCDAPDSPRPVKASGEDSGLFSPIRSSAFSPLGGCTPAECFCQTDIGGDRGHENPDSLYYTYEDYANSLSCEVLGSVLHSQHTNATSNTNSVKKGENKMVAFQHGSLDQKSKSKNKPIMIDSVQKFAADLVEKSFGSAFKDLQKGVSSCTNALCQLAIKLTSSIFQMAFNELRRQCAFSLKERAISGLASFLVSEAFSNALKDLQYVKKQIFTNTVTRFAADLAEELVFEGIMEVCQFSYPPTPASLQGRSFHCEDRVVKSYARDLSESVIQEAFIELSQVDVTFTTKAAVSVSPENIKFVSTEDVVPATQTSLCSSTSNSQTVTVTKPVQEYKKEYTVQQALFCTSGIVTSIPVPLAGSALHPYRISSNTYQTKSHPSCDDSHLNGGPTPASVATKNKEEELACLRNICLPSDHNPGSQNEVKPTSDVVETKSSSKLTNDPVIIGDFSAAMVHTIVNETIDSLTSSKVAKTEEHTDCLTQTGKGKTPSFYWDQATVPHSEARKKDMFADRLSKSIIKHSIDESKSVILNVDKNVLPKKDLPVSGDESQLTLEEFPKFLEAQDHLTHCSLSEVKDCVPEGKGSVAPGFSLETLPPCLAAAGQKADLKEIAKDKSVKKQNLHNSALEPLAFGQENSFPHSHAFSSTVLTCVDGLHVEDKQKMRDGNVIPDTPPSTPLVPSQAPSDWDIKKLTKKLKGELAKEFAPATPPSTPHNSSVSSLSENEQNAIEKEEFMLKLMRSLSEEVESSDGEEQQEMDVKLEPAGKKVQFADALATHIVSLATEMAASHLDNKLIQEAGVTSPCLNVQSQRSLSPAFLNCSDESLQALCSFAGDMAAEVITEAEKIAKVRSCMFFRRKRNSCVDGDQDRRSEEKLDVEAVAHPGGVDPFVLPLPPSSCLSGLTYKYPSCESVTDEYAGHIIQILKQEGGNSELIMDQYANRLAYRSVKSGLQEAAKTAKVKCSSKILPVQSSQVKTNDELLLFLNKEHQQEADKKRQSRSAASYLHKNRTCEWTRDMYRNECLELRSFSTCLAHSITRDVKKELMASTGGLPKSLTDSCLYEKNVCDEDTETHVGPEFPKSLQPSSQNHRFSQSTGSLSGCGYGETVVQAIEQYANKVVGDTLELGLGSAIFHVSEATKAVDRITYAEKLSPLVGQACRYCDRKELHDCTRNSSPHFPRPDSLAGSKPVSHSQLSSFYQKSRIFHLDVPQIHVDLDKKTVLAEKIVAEAIEKAERELSSTSQAADSGIGQEGVSFAESLSTEILASAVVSAGQAISSPKETEEIQSAESLGSQQMHLSIGEDSTGSWSNLSFEDEHQDESSSFHHLSESDGPDDDEEERQTDVEGLEQDGKTLLITNIDMEPCTVDPQLRIILQWLVASEAEVAELYFHDSTKKEFMQLSKRLQEKGWKVGDLLQAVLKYYEEVEKKSSEERCKSLFDWLLENA

Target ORF information:

RefSeq Version	XM_006076503.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006076503.2

ORF Insert Sequence:

ATGGCGACCT TCCAGTCTTT CAGAAACAAT CACATGAAAA CTAGAGCATC TGTCAGAAAA 
AGCTTCAATG AAGATGTGTT CCAGTCTGTG AAGTCTTTAT TGCAGAGCGA GAAGGAACTA 
TGCAGTGTAT CAGCCGAGGA CTGTTTGAAG CAGGATGAAC ATGCCAATTT GACTGAGGTC 
ACATTTCTAG GTTTTAATGA AGAAACAGAT GCTGCTCATA TACAGGATTT GGCTGCAGTT 
TCTTTGGAAC TCCCAGATCT TCTGAACTCA CTGCACTTCT GCAGTCTAAA TGAAAATGAA 
ATTATTTGTA TGAAGGATAT AAATAAATCA TCAGATATAA ACAGTGATCC TCTAAACCAG 
AGTCAACATC CTGGAATGCT TTGTGTCATG AGAGTGTCAC CTACATTACC AAGACTTAAA 
ATTGATTTTA TTTTTAGTCT CCTAAGTAAA TATGCTACCG GCATAAGGTA CACCCTGGAC 
ACATATTTGC ATCACAAGCA CCAACTTGAG GCCACCAATG AAGACGACGA TGATACTAAC 
CAGTCTGTAT CTTCCATCGA AGATGACTTT GTCACTGCTT TTGAGCACTT AGACGAGGAA 
GAGACTTCAA AGCCATATAA TGATGGATTA AACATAATTG CACTAAGGAG CCAGTGTGAT 
GCTGCTTCAC AGACTATTTC TGGTCACCAT TTGGATACCC ATGATTTCAG GGTTCTGGTT 
GGCTCTGAGC AGCAGAAGTC ATTGGCTAAA CCTTCAGCTT CTTTAATAAA CATTCTGGGA 
CATAAAAAAC TGCCTCCAGC GAGAACTTCA GTCACGACAT CAGTCTCTGA ACCATGGATC 
CAGAGGAGTT TCTATAGTTC CTTTGCTGCT TCAGAGAAAG GTAGTGATGC CCAGAAACCA 
TTGTTCTCGT CTTCTCCTGC CTACTCGTCT GAATCAGAGT GCTCAAGTCC AAGTCCTGTT 
ATTTTCTTGG ATGAAGAAGG TTATCAAAAA AGTTTGAAAG CAAAACTTGA GTTACCTAAA 
ATTCCTGTGA TGAAAGATGA TATTGAGGAT TCAGACTCAG AAGTGAGTGA GTTTTTTGAT 
AGTTTTGATC AGTTTGATGA ACTAGAACAA ACGTTAGAGA CTGCTTGTCC GTTTCTGAAA 
GATCTTGCTG TAGGGAAGCC ATCGCAAAAG AAAGGGCACA AACATGAAAA ATTGTGCTCT 
GTAACCACTA CTATGAATCC TCAAAAATTC AAGTTTGATC GGCCAGCTCT CCCAGCTAAT 
GTTAGGAAGC CAACACCTCG TAAACCAGAA TCCCCTTATA GTAACCTGTG TGATGCCCCA 
GATTCTCCCC GCCCCGTGAA GGCCTCAGGG GAAGACAGTG GTTTGTTTAG TCCTATTCGA 
TCCTCTGCTT TCAGTCCTCT TGGAGGCTGT ACTCCAGCTG AATGCTTTTG CCAAACAGAT 
ATTGGTGGAG ATAGGGGTCA TGAAAATCCT GATTCTCTTT ATTATACCTA TGAAGATTAT 
GCAAATAGCC TTTCCTGTGA AGTTCTGGGC TCAGTTCTTC ATAGCCAACA TACTAATGCA 
ACATCCAACA CTAATAGTGT TAAAAAGGGA GAAAATAAAA TGGTAGCTTT TCAGCATGGA 
AGCCTTGATC AAAAAAGTAA ATCTAAAAAT AAACCCATAA TGATTGATAG CGTTCAGAAG 
TTTGCAGCAG ATCTCGTGGA AAAAAGTTTT GGCAGTGCAT TTAAAGACTT ACAGAAAGGG 
GTCTCTTCAT GTACCAATGC CTTATGCCAG TTAGCCATCA AATTGACGTC ATCCATTTTT 
CAAATGGCAT TTAACGAACT GAGAAGGCAG TGTGCCTTTT CACTGAAAGA ACGAGCCATT 
AGTGGCCTGG CTAGTTTTTT GGTGAGTGAG GCATTCTCAA ATGCTTTAAA AGATCTACAA 
TATGTAAAGA AACAGATCTT CACTAACACC GTCACTAGAT TTGCTGCAGA TCTTGCTGAA 
GAGCTTGTTT TTGAAGGCAT CATGGAAGTG TGTCAGTTCT CATATCCTCC AACACCTGCA 
TCTCTGCAGG GTCGGTCATT TCACTGTGAA GACAGAGTGG TGAAGTCCTA TGCAAGAGAT 
CTGTCTGAAT CTGTAATACA GGAAGCATTC ATTGAGCTGT CTCAAGTTGA TGTTACCTTT 
ACCACAAAGG CAGCAGTTAG TGTTTCCCCA GAAAACATCA AGTTTGTGAG CACAGAAGAT 
GTAGTGCCAG CAACACAGAC TTCTTTGTGT TCTTCTACTT CTAACAGTCA AACAGTTACG 
GTGACAAAAC CAGTGCAGGA GTATAAAAAG GAATACACGG TGCAGCAGGC CTTGTTCTGT 
ACTTCAGGAA TTGTTACTTC AATACCAGTG CCCTTGGCAG GAAGTGCCCT TCACCCATAC 
CGTATTTCTT CTAATACGTA TCAGACAAAG TCTCATCCAT CATGTGATGA TAGTCATTTG 
AATGGTGGTC CTACCCCAGC AAGTGTTGCC ACAAAAAACA AAGAAGAGGA GCTAGCTTGT 
CTCAGGAATA TTTGTTTACC TTCAGACCAC AACCCCGGTA GCCAGAATGA GGTGAAACCA 
ACTAGTGATG TTGTTGAAAC AAAAAGCTCT TCAAAGTTAA CAAATGATCC TGTCATTATT 
GGCGATTTTT CTGCCGCAAT GGTGCATACG ATAGTAAATG AAACTATAGA TTCACTGACA 
TCTTCCAAAG TTGCAAAAAC AGAAGAACAC ACAGATTGTT TAACTCAAAC AGGAAAGGGA 
AAAACCCCTT CTTTCTACTG GGATCAAGCA ACAGTGCCAC ACAGTGAGGC TAGAAAGAAG 
GACATGTTCG CTGATCGGTT ATCTAAATCT ATTATCAAAC ATTCCATAGA TGAAAGCAAG 
TCAGTGATCC TAAATGTAGA TAAAAATGTC CTCCCCAAGA AAGACTTGCC TGTTTCTGGA 
GATGAGTCAC AGTTGACCTT GGAAGAGTTC CCCAAGTTTC TTGAAGCTCA AGATCATTTA 
ACTCACTGTT CACTTTCAGA AGTAAAGGAT TGTGTTCCAG AAGGTAAAGG TTCTGTGGCT 
CCTGGATTTT CTTTGGAGAC GCTACCACCT TGTCTAGCAG CGGCAGGCCA GAAAGCTGAT 
CTGAAGGAAA TTGCTAAGGA CAAATCTGTG AAAAAGCAGA ATTTGCATAA TTCAGCCCTC 
GAGCCCTTGG CTTTTGGGCA GGAAAACTCT TTTCCCCATT CACATGCTTT CTCATCCACA 
GTGCTCACCT GTGTAGATGG TTTGCATGTG GAAGATAAAC AGAAAATGAG AGATGGGAAT 
GTAATACCTG ACACCCCTCC ATCAACTCCT CTGGTTCCAT CCCAGGCTCC TTCTGATTGG 
GATATCAAGA AGTTAACCAA AAAGCTTAAG GGGGAGTTAG CAAAAGAGTT TGCACCGGCC 
ACACCACCAT CCACACCTCA CAACTCATCT GTAAGCAGTT TGTCTGAAAA TGAACAAAAT 
GCAATAGAAA AAGAAGAGTT CATGTTGAAG CTCATGCGGT CTCTCTCAGA AGAAGTTGAA 
AGTAGTGACG GCGAAGAGCA GCAAGAGATG GACGTGAAAT TGGAGCCAGC AGGGAAAAAA 
GTTCAGTTTG CAGACGCATT AGCGACGCAC ATCGTTTCTC TTGCAACCGA AATGGCAGCT 
TCCCATTTGG ATAATAAATT GATTCAAGAA GCCGGGGTGA CAAGTCCTTG CTTAAATGTG 
CAGAGTCAAA GAAGCCTATC ACCTGCTTTT TTAAATTGCT CAGATGAAAG CTTACAAGCA 
TTATGCAGTT TTGCAGGTGA TATGGCAGCA GAAGTCATTA CAGAAGCTGA AAAAATAGCA 
AAAGTTAGGA GCTGTATGTT TTTCAGGAGG AAGAGGAACA GTTGTGTTGA TGGTGACCAA 
GATCGTAGAT CCGAAGAGAA GTTGGATGTA GAGGCTGTAG CGCACCCGGG AGGAGTAGAT 
CCGTTTGTTC TTCCTTTACC ACCAAGTTCT TGTCTGTCAG GTCTAACGTA CAAGTATCCC 
AGCTGTGAAA GTGTGACAGA TGAGTATGCA GGTCACATTA TCCAAATACT AAAACAGGAA 
GGTGGCAATA GTGAGTTAAT AATGGACCAG TACGCCAACA GACTTGCCTA TCGGTCTGTT 
AAGTCAGGAT TGCAAGAAGC AGCTAAAACA GCCAAAGTGA AGTGCAGCTC CAAAATACTC 
CCTGTGCAGA GCTCACAGGT GAAAACCAAC GATGAACTGT TACTGTTTTT AAATAAAGAA 
CACCAGCAAG AAGCAGATAA AAAAAGACAA AGCAGAAGTG CTGCAAGTTA CCTTCATAAA 
AACCGGACTT GTGAGTGGAC ACGGGACATG TACAGAAACG AGTGCTTGGA ACTACGTAGT 
TTTTCAACCT GTCTTGCTCA CAGCATCACA AGAGATGTTA AAAAAGAGTT GATGGCATCA 
ACAGGTGGCT TGCCAAAATC CTTAACAGAT TCTTGTCTTT ATGAAAAAAA CGTATGTGAC 
GAAGATACTG AGACTCATGT CGGGCCAGAG TTTCCCAAGT CTCTTCAGCC TTCTTCACAG 
AATCACAGAT TTTCTCAGAG TACAGGCAGC TTGAGTGGGT GTGGCTATGG AGAGACTGTC 
GTTCAAGCTA TAGAGCAGTA TGCTAACAAA GTCGTGGGCG ACACTTTAGA GCTGGGTTTA 
GGATCTGCCA TCTTCCACGT GTCTGAAGCC ACAAAAGCAG TTGATAGGAT TACTTACGCA 
GAAAAGCTGT CACCTCTGGT GGGTCAGGCT TGCCGATACT GTGACCGTAA AGAGCTCCAT 
GACTGCACTA GAAATTCGTC TCCTCACTTT CCCAGACCGG ATTCACTTGC TGGTAGTAAG 
CCAGTTTCTC ATTCACAGCT TAGCAGCTTC TATCAGAAAT CTAGGATTTT TCATCTTGAT 
GTTCCTCAAA TTCACGTTGA TCTCGATAAG AAGACAGTGC TTGCTGAGAA GATAGTTGCT 
GAAGCGATTG AAAAGGCAGA GAGAGAGCTG AGCAGTACCA GCCAGGCAGC CGACAGTGGG 
ATCGGACAGG AAGGTGTCAG CTTTGCCGAG AGCCTCAGCA CAGAGATACT GGCGTCAGCA 
GTGGTCAGTG CTGGACAGGC CATCAGCAGT CCAAAAGAAA CAGAAGAGAT TCAGTCAGCT 
GAGTCTCTTG GTAGCCAGCA GATGCATCTC AGTATTGGTG AGGACAGTAC TGGGAGCTGG 
TCCAACTTAA GTTTTGAAGA TGAACACCAG GATGAAAGCA GTAGTTTTCA CCATCTAAGT 
GAAAGTGATG GGCCAGATGA TGATGAAGAG GAGCGTCAGA CTGATGTAGA AGGTTTGGAG 
CAAGATGGAA AGACTCTGCT AATTACAAAT ATTGACATGG AGCCATGCAC AGTAGACCCC 
CAGCTAAGGA TTATTCTTCA GTGGCTCGTT GCTTCTGAGG CAGAAGTTGC AGAACTTTAT 
TTTCATGACT CTACAAAGAA GGAGTTTATG CAACTTTCAA AACGGCTACA GGAAAAAGGG 
TGGAAGGTGG GAGACCTCCT GCAGGCTGTG CTTAAATACT ATGAGGAGGT GGAGAAGAAG 
TCCAGTGAGG AGAGATGCAA GTCCCTGTTT GATTGGCTCT TGGAAAATGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP11 ( XM_006076503.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006076565.1 Bubalus bubalis A kinase (PRKA) anchor protein 11 (AKAP11), transcript variant X2, mRNA.

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CloneID	OBu30011
Clone ID Related Accession (Same CDS sequence)	XM_006076503.2 , XM_006076503.1
Accession Version	XM_006076503.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	A-kinase anchor protein 11 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785696.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCT TCCAGTCTTT CAGAAACAAT CACATGAAAA CTAGAGCATC TGTCAGAAAA 
AGCTTCAATG AAGATGTGTT CCAGTCTGTG AAGTCTTTAT TGCAGAGCGA GAAGGAACTA 
TGCAGTGTAT CAGCCGAGGA CTGTTTGAAG CAGGATGAAC ATGCCAATTT GACTGAGGTC 
ACATTTCTAG GTTTTAATGA AGAAACAGAT GCTGCTCATA TACAGGATTT GGCTGCAGTT 
TCTTTGGAAC TCCCAGATCT TCTGAACTCA CTGCACTTCT GCAGTCTAAA TGAAAATGAA 
ATTATTTGTA TGAAGGATAT AAATAAATCA TCAGATATAA ACAGTGATCC TCTAAACCAG 
AGTCAACATC CTGGAATGCT TTGTGTCATG AGAGTGTCAC CTACATTACC AAGACTTAAA 
ATTGATTTTA TTTTTAGTCT CCTAAGTAAA TATGCTACCG GCATAAGGTA CACCCTGGAC 
ACATATTTGC ATCACAAGCA CCAACTTGAG GCCACCAATG AAGACGACGA TGATACTAAC 
CAGTCTGTAT CTTCCATCGA AGATGACTTT GTCACTGCTT TTGAGCACTT AGACGAGGAA 
GAGACTTCAA AGCCATATAA TGATGGATTA AACATAATTG CACTAAGGAG CCAGTGTGAT 
GCTGCTTCAC AGACTATTTC TGGTCACCAT TTGGATACCC ATGATTTCAG GGTTCTGGTT 
GGCTCTGAGC AGCAGAAGTC ATTGGCTAAA CCTTCAGCTT CTTTAATAAA CATTCTGGGA 
CATAAAAAAC TGCCTCCAGC GAGAACTTCA GTCACGACAT CAGTCTCTGA ACCATGGATC 
CAGAGGAGTT TCTATAGTTC CTTTGCTGCT TCAGAGAAAG GTAGTGATGC CCAGAAACCA 
TTGTTCTCGT CTTCTCCTGC CTACTCGTCT GAATCAGAGT GCTCAAGTCC AAGTCCTGTT 
ATTTTCTTGG ATGAAGAAGG TTATCAAAAA AGTTTGAAAG CAAAACTTGA GTTACCTAAA 
ATTCCTGTGA TGAAAGATGA TATTGAGGAT TCAGACTCAG AAGTGAGTGA GTTTTTTGAT 
AGTTTTGATC AGTTTGATGA ACTAGAACAA ACGTTAGAGA CTGCTTGTCC GTTTCTGAAA 
GATCTTGCTG TAGGGAAGCC ATCGCAAAAG AAAGGGCACA AACATGAAAA ATTGTGCTCT 
GTAACCACTA CTATGAATCC TCAAAAATTC AAGTTTGATC GGCCAGCTCT CCCAGCTAAT 
GTTAGGAAGC CAACACCTCG TAAACCAGAA TCCCCTTATA GTAACCTGTG TGATGCCCCA 
GATTCTCCCC GCCCCGTGAA GGCCTCAGGG GAAGACAGTG GTTTGTTTAG TCCTATTCGA 
TCCTCTGCTT TCAGTCCTCT TGGAGGCTGT ACTCCAGCTG AATGCTTTTG CCAAACAGAT 
ATTGGTGGAG ATAGGGGTCA TGAAAATCCT GATTCTCTTT ATTATACCTA TGAAGATTAT 
GCAAATAGCC TTTCCTGTGA AGTTCTGGGC TCAGTTCTTC ATAGCCAACA TACTAATGCA 
ACATCCAACA CTAATAGTGT TAAAAAGGGA GAAAATAAAA TGGTAGCTTT TCAGCATGGA 
AGCCTTGATC AAAAAAGTAA ATCTAAAAAT AAACCCATAA TGATTGATAG CGTTCAGAAG 
TTTGCAGCAG ATCTCGTGGA AAAAAGTTTT GGCAGTGCAT TTAAAGACTT ACAGAAAGGG 
GTCTCTTCAT GTACCAATGC CTTATGCCAG TTAGCCATCA AATTGACGTC ATCCATTTTT 
CAAATGGCAT TTAACGAACT GAGAAGGCAG TGTGCCTTTT CACTGAAAGA ACGAGCCATT 
AGTGGCCTGG CTAGTTTTTT GGTGAGTGAG GCATTCTCAA ATGCTTTAAA AGATCTACAA 
TATGTAAAGA AACAGATCTT CACTAACACC GTCACTAGAT TTGCTGCAGA TCTTGCTGAA 
GAGCTTGTTT TTGAAGGCAT CATGGAAGTG TGTCAGTTCT CATATCCTCC AACACCTGCA 
TCTCTGCAGG GTCGGTCATT TCACTGTGAA GACAGAGTGG TGAAGTCCTA TGCAAGAGAT 
CTGTCTGAAT CTGTAATACA GGAAGCATTC ATTGAGCTGT CTCAAGTTGA TGTTACCTTT 
ACCACAAAGG CAGCAGTTAG TGTTTCCCCA GAAAACATCA AGTTTGTGAG CACAGAAGAT 
GTAGTGCCAG CAACACAGAC TTCTTTGTGT TCTTCTACTT CTAACAGTCA AACAGTTACG 
GTGACAAAAC CAGTGCAGGA GTATAAAAAG GAATACACGG TGCAGCAGGC CTTGTTCTGT 
ACTTCAGGAA TTGTTACTTC AATACCAGTG CCCTTGGCAG GAAGTGCCCT TCACCCATAC 
CGTATTTCTT CTAATACGTA TCAGACAAAG TCTCATCCAT CATGTGATGA TAGTCATTTG 
AATGGTGGTC CTACCCCAGC AAGTGTTGCC ACAAAAAACA AAGAAGAGGA GCTAGCTTGT 
CTCAGGAATA TTTGTTTACC TTCAGACCAC AACCCCGGTA GCCAGAATGA GGTGAAACCA 
ACTAGTGATG TTGTTGAAAC AAAAAGCTCT TCAAAGTTAA CAAATGATCC TGTCATTATT 
GGCGATTTTT CTGCCGCAAT GGTGCATACG ATAGTAAATG AAACTATAGA TTCACTGACA 
TCTTCCAAAG TTGCAAAAAC AGAAGAACAC ACAGATTGTT TAACTCAAAC AGGAAAGGGA 
AAAACCCCTT CTTTCTACTG GGATCAAGCA ACAGTGCCAC ACAGTGAGGC TAGAAAGAAG 
GACATGTTCG CTGATCGGTT ATCTAAATCT ATTATCAAAC ATTCCATAGA TGAAAGCAAG 
TCAGTGATCC TAAATGTAGA TAAAAATGTC CTCCCCAAGA AAGACTTGCC TGTTTCTGGA 
GATGAGTCAC AGTTGACCTT GGAAGAGTTC CCCAAGTTTC TTGAAGCTCA AGATCATTTA 
ACTCACTGTT CACTTTCAGA AGTAAAGGAT TGTGTTCCAG AAGGTAAAGG TTCTGTGGCT 
CCTGGATTTT CTTTGGAGAC GCTACCACCT TGTCTAGCAG CGGCAGGCCA GAAAGCTGAT 
CTGAAGGAAA TTGCTAAGGA CAAATCTGTG AAAAAGCAGA ATTTGCATAA TTCAGCCCTC 
GAGCCCTTGG CTTTTGGGCA GGAAAACTCT TTTCCCCATT CACATGCTTT CTCATCCACA 
GTGCTCACCT GTGTAGATGG TTTGCATGTG GAAGATAAAC AGAAAATGAG AGATGGGAAT 
GTAATACCTG ACACCCCTCC ATCAACTCCT CTGGTTCCAT CCCAGGCTCC TTCTGATTGG 
GATATCAAGA AGTTAACCAA AAAGCTTAAG GGGGAGTTAG CAAAAGAGTT TGCACCGGCC 
ACACCACCAT CCACACCTCA CAACTCATCT GTAAGCAGTT TGTCTGAAAA TGAACAAAAT 
GCAATAGAAA AAGAAGAGTT CATGTTGAAG CTCATGCGGT CTCTCTCAGA AGAAGTTGAA 
AGTAGTGACG GCGAAGAGCA GCAAGAGATG GACGTGAAAT TGGAGCCAGC AGGGAAAAAA 
GTTCAGTTTG CAGACGCATT AGCGACGCAC ATCGTTTCTC TTGCAACCGA AATGGCAGCT 
TCCCATTTGG ATAATAAATT GATTCAAGAA GCCGGGGTGA CAAGTCCTTG CTTAAATGTG 
CAGAGTCAAA GAAGCCTATC ACCTGCTTTT TTAAATTGCT CAGATGAAAG CTTACAAGCA 
TTATGCAGTT TTGCAGGTGA TATGGCAGCA GAAGTCATTA CAGAAGCTGA AAAAATAGCA 
AAAGTTAGGA GCTGTATGTT TTTCAGGAGG AAGAGGAACA GTTGTGTTGA TGGTGACCAA 
GATCGTAGAT CCGAAGAGAA GTTGGATGTA GAGGCTGTAG CGCACCCGGG AGGAGTAGAT 
CCGTTTGTTC TTCCTTTACC ACCAAGTTCT TGTCTGTCAG GTCTAACGTA CAAGTATCCC 
AGCTGTGAAA GTGTGACAGA TGAGTATGCA GGTCACATTA TCCAAATACT AAAACAGGAA 
GGTGGCAATA GTGAGTTAAT AATGGACCAG TACGCCAACA GACTTGCCTA TCGGTCTGTT 
AAGTCAGGAT TGCAAGAAGC AGCTAAAACA GCCAAAGTGA AGTGCAGCTC CAAAATACTC 
CCTGTGCAGA GCTCACAGGT GAAAACCAAC GATGAACTGT TACTGTTTTT AAATAAAGAA 
CACCAGCAAG AAGCAGATAA AAAAAGACAA AGCAGAAGTG CTGCAAGTTA CCTTCATAAA 
AACCGGACTT GTGAGTGGAC ACGGGACATG TACAGAAACG AGTGCTTGGA ACTACGTAGT 
TTTTCAACCT GTCTTGCTCA CAGCATCACA AGAGATGTTA AAAAAGAGTT GATGGCATCA 
ACAGGTGGCT TGCCAAAATC CTTAACAGAT TCTTGTCTTT ATGAAAAAAA CGTATGTGAC 
GAAGATACTG AGACTCATGT CGGGCCAGAG TTTCCCAAGT CTCTTCAGCC TTCTTCACAG 
AATCACAGAT TTTCTCAGAG TACAGGCAGC TTGAGTGGGT GTGGCTATGG AGAGACTGTC 
GTTCAAGCTA TAGAGCAGTA TGCTAACAAA GTCGTGGGCG ACACTTTAGA GCTGGGTTTA 
GGATCTGCCA TCTTCCACGT GTCTGAAGCC ACAAAAGCAG TTGATAGGAT TACTTACGCA 
GAAAAGCTGT CACCTCTGGT GGGTCAGGCT TGCCGATACT GTGACCGTAA AGAGCTCCAT 
GACTGCACTA GAAATTCGTC TCCTCACTTT CCCAGACCGG ATTCACTTGC TGGTAGTAAG 
CCAGTTTCTC ATTCACAGCT TAGCAGCTTC TATCAGAAAT CTAGGATTTT TCATCTTGAT 
GTTCCTCAAA TTCACGTTGA TCTCGATAAG AAGACAGTGC TTGCTGAGAA GATAGTTGCT 
GAAGCGATTG AAAAGGCAGA GAGAGAGCTG AGCAGTACCA GCCAGGCAGC CGACAGTGGG 
ATCGGACAGG AAGGTGTCAG CTTTGCCGAG AGCCTCAGCA CAGAGATACT GGCGTCAGCA 
GTGGTCAGTG CTGGACAGGC CATCAGCAGT CCAAAAGAAA CAGAAGAGAT TCAGTCAGCT 
GAGTCTCTTG GTAGCCAGCA GATGCATCTC AGTATTGGTG AGGACAGTAC TGGGAGCTGG 
TCCAACTTAA GTTTTGAAGA TGAACACCAG GATGAAAGCA GTAGTTTTCA CCATCTAAGT 
GAAAGTGATG GGCCAGATGA TGATGAAGAG GAGCGTCAGA CTGATGTAGA AGGTTTGGAG 
CAAGATGGAA AGACTCTGCT AATTACAAAT ATTGACATGG AGCCATGCAC AGTAGACCCC 
CAGCTAAGGA TTATTCTTCA GTGGCTCGTT GCTTCTGAGG CAGAAGTTGC AGAACTTTAT 
TTTCATGACT CTACAAAGAA GGAGTTTATG CAACTTTCAA AACGGCTACA GGAAAAAGGG 
TGGAAGGTGG GAGACCTCCT GCAGGCTGTG CTTAAATACT ATGAGGAGGT GGAGAAGAAG 
TCCAGTGAGG AGAGATGCAA GTCCCTGTTT GATTGGCTCT TGGAAAATGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006076565.1
CDS	96..5729
Translation	MATFQSFRNNHMKTRASVRKSFNEDVFQSVKSLLQSEKELCSVSAEDCLKQDEHANLTEVTFLGFNEETDAAHIQDLAAVSLELPDLLNSLHFCSLNENEIICMKDINKSSDINSDPLNQSQHPGMLCVMRVSPTLPRLKIDFIFSLLSKYATGIRYTLDTYLHHKHQLEATNEDDDDTNQSVSSIEDDFVTAFEHLDEEETSKPYNDGLNIIALRSQCDAASQTISGHHLDTHDFRVLVGSEQQKSLAKPSASLINILGHKKLPPARTSVTTSVSEPWIQRSFYSSFAASEKGSDAQKPLFSSSPAYSSESECSSPSPVIFLDEEGYQKSLKAKLELPKIPVMKDDIEDSDSEVSEFFDSFDQFDELEQTLETACPFLKDLAVGKPSQKKGHKHEKLCSVTTTMNPQKFKFDRPALPANVRKPTPRKPESPYSNLCDAPDSPRPVKASGEDSGLFSPIRSSAFSPLGGCTPAECFCQTDIGGDRGHENPDSLYYTYEDYANSLSCEVLGSVLHSQHTNATSNTNSVKKGENKMVAFQHGSLDQKSKSKNKPIMIDSVQKFAADLVEKSFGSAFKDLQKGVSSCTNALCQLAIKLTSSIFQMAFNELRRQCAFSLKERAISGLASFLVSEAFSNALKDLQYVKKQIFTNTVTRFAADLAEELVFEGIMEVCQFSYPPTPASLQGRSFHCEDRVVKSYARDLSESVIQEAFIELSQVDVTFTTKAAVSVSPENIKFVSTEDVVPATQTSLCSSTSNSQTVTVTKPVQEYKKEYTVQQALFCTSGIVTSIPVPLAGSALHPYRISSNTYQTKSHPSCDDSHLNGGPTPASVATKNKEEELACLRNICLPSDHNPGSQNEVKPTSDVVETKSSSKLTNDPVIIGDFSAAMVHTIVNETIDSLTSSKVAKTEEHTDCLTQTGKGKTPSFYWDQATVPHSEARKKDMFADRLSKSIIKHSIDESKSVILNVDKNVLPKKDLPVSGDESQLTLEEFPKFLEAQDHLTHCSLSEVKDCVPEGKGSVAPGFSLETLPPCLAAAGQKADLKEIAKDKSVKKQNLHNSALEPLAFGQENSFPHSHAFSSTVLTCVDGLHVEDKQKMRDGNVIPDTPPSTPLVPSQAPSDWDIKKLTKKLKGELAKEFAPATPPSTPHNSSVSSLSENEQNAIEKEEFMLKLMRSLSEEVESSDGEEQQEMDVKLEPAGKKVQFADALATHIVSLATEMAASHLDNKLIQEAGVTSPCLNVQSQRSLSPAFLNCSDESLQALCSFAGDMAAEVITEAEKIAKVRSCMFFRRKRNSCVDGDQDRRSEEKLDVEAVAHPGGVDPFVLPLPPSSCLSGLTYKYPSCESVTDEYAGHIIQILKQEGGNSELIMDQYANRLAYRSVKSGLQEAAKTAKVKCSSKILPVQSSQVKTNDELLLFLNKEHQQEADKKRQSRSAASYLHKNRTCEWTRDMYRNECLELRSFSTCLAHSITRDVKKELMASTGGLPKSLTDSCLYEKNVCDEDTETHVGPEFPKSLQPSSQNHRFSQSTGSLSGCGYGETVVQAIEQYANKVVGDTLELGLGSAIFHVSEATKAVDRITYAEKLSPLVGQACRYCDRKELHDCTRNSSPHFPRPDSLAGSKPVSHSQLSSFYQKSRIFHLDVPQIHVDLDKKTVLAEKIVAEAIEKAERELSSTSQAADSGIGQEGVSFAESLSTEILASAVVSAGQAISSPKETEEIQSAESLGSQQMHLSIGEDSTGSWSNLSFEDEHQDESSSFHHLSESDGPDDDEEERQTDVEGLEQDGKTLLITNIDMEPCTVDPQLRIILQWLVASEAEVAELYFHDSTKKEFMQLSKRLQEKGWKVGDLLQAVLKYYEEVEKKSSEERCKSLFDWLLENA

Target ORF information:

RefSeq Version	XM_006076503.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis A kinase (PRKA) anchor protein 11 (AKAP11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006076503.1

ORF Insert Sequence:

ATGGCGACCT TCCAGTCTTT CAGAAACAAT CACATGAAAA CTAGAGCATC TGTCAGAAAA 
AGCTTCAATG AAGATGTGTT CCAGTCTGTG AAGTCTTTAT TGCAGAGCGA GAAGGAACTA 
TGCAGTGTAT CAGCCGAGGA CTGTTTGAAG CAGGATGAAC ATGCCAATTT GACTGAGGTC 
ACATTTCTAG GTTTTAATGA AGAAACAGAT GCTGCTCATA TACAGGATTT GGCTGCAGTT 
TCTTTGGAAC TCCCAGATCT TCTGAACTCA CTGCACTTCT GCAGTCTAAA TGAAAATGAA 
ATTATTTGTA TGAAGGATAT AAATAAATCA TCAGATATAA ACAGTGATCC TCTAAACCAG 
AGTCAACATC CTGGAATGCT TTGTGTCATG AGAGTGTCAC CTACATTACC AAGACTTAAA 
ATTGATTTTA TTTTTAGTCT CCTAAGTAAA TATGCTACCG GCATAAGGTA CACCCTGGAC 
ACATATTTGC ATCACAAGCA CCAACTTGAG GCCACCAATG AAGACGACGA TGATACTAAC 
CAGTCTGTAT CTTCCATCGA AGATGACTTT GTCACTGCTT TTGAGCACTT AGACGAGGAA 
GAGACTTCAA AGCCATATAA TGATGGATTA AACATAATTG CACTAAGGAG CCAGTGTGAT 
GCTGCTTCAC AGACTATTTC TGGTCACCAT TTGGATACCC ATGATTTCAG GGTTCTGGTT 
GGCTCTGAGC AGCAGAAGTC ATTGGCTAAA CCTTCAGCTT CTTTAATAAA CATTCTGGGA 
CATAAAAAAC TGCCTCCAGC GAGAACTTCA GTCACGACAT CAGTCTCTGA ACCATGGATC 
CAGAGGAGTT TCTATAGTTC CTTTGCTGCT TCAGAGAAAG GTAGTGATGC CCAGAAACCA 
TTGTTCTCGT CTTCTCCTGC CTACTCGTCT GAATCAGAGT GCTCAAGTCC AAGTCCTGTT 
ATTTTCTTGG ATGAAGAAGG TTATCAAAAA AGTTTGAAAG CAAAACTTGA GTTACCTAAA 
ATTCCTGTGA TGAAAGATGA TATTGAGGAT TCAGACTCAG AAGTGAGTGA GTTTTTTGAT 
AGTTTTGATC AGTTTGATGA ACTAGAACAA ACGTTAGAGA CTGCTTGTCC GTTTCTGAAA 
GATCTTGCTG TAGGGAAGCC ATCGCAAAAG AAAGGGCACA AACATGAAAA ATTGTGCTCT 
GTAACCACTA CTATGAATCC TCAAAAATTC AAGTTTGATC GGCCAGCTCT CCCAGCTAAT 
GTTAGGAAGC CAACACCTCG TAAACCAGAA TCCCCTTATA GTAACCTGTG TGATGCCCCA 
GATTCTCCCC GCCCCGTGAA GGCCTCAGGG GAAGACAGTG GTTTGTTTAG TCCTATTCGA 
TCCTCTGCTT TCAGTCCTCT TGGAGGCTGT ACTCCAGCTG AATGCTTTTG CCAAACAGAT 
ATTGGTGGAG ATAGGGGTCA TGAAAATCCT GATTCTCTTT ATTATACCTA TGAAGATTAT 
GCAAATAGCC TTTCCTGTGA AGTTCTGGGC TCAGTTCTTC ATAGCCAACA TACTAATGCA 
ACATCCAACA CTAATAGTGT TAAAAAGGGA GAAAATAAAA TGGTAGCTTT TCAGCATGGA 
AGCCTTGATC AAAAAAGTAA ATCTAAAAAT AAACCCATAA TGATTGATAG CGTTCAGAAG 
TTTGCAGCAG ATCTCGTGGA AAAAAGTTTT GGCAGTGCAT TTAAAGACTT ACAGAAAGGG 
GTCTCTTCAT GTACCAATGC CTTATGCCAG TTAGCCATCA AATTGACGTC ATCCATTTTT 
CAAATGGCAT TTAACGAACT GAGAAGGCAG TGTGCCTTTT CACTGAAAGA ACGAGCCATT 
AGTGGCCTGG CTAGTTTTTT GGTGAGTGAG GCATTCTCAA ATGCTTTAAA AGATCTACAA 
TATGTAAAGA AACAGATCTT CACTAACACC GTCACTAGAT TTGCTGCAGA TCTTGCTGAA 
GAGCTTGTTT TTGAAGGCAT CATGGAAGTG TGTCAGTTCT CATATCCTCC AACACCTGCA 
TCTCTGCAGG GTCGGTCATT TCACTGTGAA GACAGAGTGG TGAAGTCCTA TGCAAGAGAT 
CTGTCTGAAT CTGTAATACA GGAAGCATTC ATTGAGCTGT CTCAAGTTGA TGTTACCTTT 
ACCACAAAGG CAGCAGTTAG TGTTTCCCCA GAAAACATCA AGTTTGTGAG CACAGAAGAT 
GTAGTGCCAG CAACACAGAC TTCTTTGTGT TCTTCTACTT CTAACAGTCA AACAGTTACG 
GTGACAAAAC CAGTGCAGGA GTATAAAAAG GAATACACGG TGCAGCAGGC CTTGTTCTGT 
ACTTCAGGAA TTGTTACTTC AATACCAGTG CCCTTGGCAG GAAGTGCCCT TCACCCATAC 
CGTATTTCTT CTAATACGTA TCAGACAAAG TCTCATCCAT CATGTGATGA TAGTCATTTG 
AATGGTGGTC CTACCCCAGC AAGTGTTGCC ACAAAAAACA AAGAAGAGGA GCTAGCTTGT 
CTCAGGAATA TTTGTTTACC TTCAGACCAC AACCCCGGTA GCCAGAATGA GGTGAAACCA 
ACTAGTGATG TTGTTGAAAC AAAAAGCTCT TCAAAGTTAA CAAATGATCC TGTCATTATT 
GGCGATTTTT CTGCCGCAAT GGTGCATACG ATAGTAAATG AAACTATAGA TTCACTGACA 
TCTTCCAAAG TTGCAAAAAC AGAAGAACAC ACAGATTGTT TAACTCAAAC AGGAAAGGGA 
AAAACCCCTT CTTTCTACTG GGATCAAGCA ACAGTGCCAC ACAGTGAGGC TAGAAAGAAG 
GACATGTTCG CTGATCGGTT ATCTAAATCT ATTATCAAAC ATTCCATAGA TGAAAGCAAG 
TCAGTGATCC TAAATGTAGA TAAAAATGTC CTCCCCAAGA AAGACTTGCC TGTTTCTGGA 
GATGAGTCAC AGTTGACCTT GGAAGAGTTC CCCAAGTTTC TTGAAGCTCA AGATCATTTA 
ACTCACTGTT CACTTTCAGA AGTAAAGGAT TGTGTTCCAG AAGGTAAAGG TTCTGTGGCT 
CCTGGATTTT CTTTGGAGAC GCTACCACCT TGTCTAGCAG CGGCAGGCCA GAAAGCTGAT 
CTGAAGGAAA TTGCTAAGGA CAAATCTGTG AAAAAGCAGA ATTTGCATAA TTCAGCCCTC 
GAGCCCTTGG CTTTTGGGCA GGAAAACTCT TTTCCCCATT CACATGCTTT CTCATCCACA 
GTGCTCACCT GTGTAGATGG TTTGCATGTG GAAGATAAAC AGAAAATGAG AGATGGGAAT 
GTAATACCTG ACACCCCTCC ATCAACTCCT CTGGTTCCAT CCCAGGCTCC TTCTGATTGG 
GATATCAAGA AGTTAACCAA AAAGCTTAAG GGGGAGTTAG CAAAAGAGTT TGCACCGGCC 
ACACCACCAT CCACACCTCA CAACTCATCT GTAAGCAGTT TGTCTGAAAA TGAACAAAAT 
GCAATAGAAA AAGAAGAGTT CATGTTGAAG CTCATGCGGT CTCTCTCAGA AGAAGTTGAA 
AGTAGTGACG GCGAAGAGCA GCAAGAGATG GACGTGAAAT TGGAGCCAGC AGGGAAAAAA 
GTTCAGTTTG CAGACGCATT AGCGACGCAC ATCGTTTCTC TTGCAACCGA AATGGCAGCT 
TCCCATTTGG ATAATAAATT GATTCAAGAA GCCGGGGTGA CAAGTCCTTG CTTAAATGTG 
CAGAGTCAAA GAAGCCTATC ACCTGCTTTT TTAAATTGCT CAGATGAAAG CTTACAAGCA 
TTATGCAGTT TTGCAGGTGA TATGGCAGCA GAAGTCATTA CAGAAGCTGA AAAAATAGCA 
AAAGTTAGGA GCTGTATGTT TTTCAGGAGG AAGAGGAACA GTTGTGTTGA TGGTGACCAA 
GATCGTAGAT CCGAAGAGAA GTTGGATGTA GAGGCTGTAG CGCACCCGGG AGGAGTAGAT 
CCGTTTGTTC TTCCTTTACC ACCAAGTTCT TGTCTGTCAG GTCTAACGTA CAAGTATCCC 
AGCTGTGAAA GTGTGACAGA TGAGTATGCA GGTCACATTA TCCAAATACT AAAACAGGAA 
GGTGGCAATA GTGAGTTAAT AATGGACCAG TACGCCAACA GACTTGCCTA TCGGTCTGTT 
AAGTCAGGAT TGCAAGAAGC AGCTAAAACA GCCAAAGTGA AGTGCAGCTC CAAAATACTC 
CCTGTGCAGA GCTCACAGGT GAAAACCAAC GATGAACTGT TACTGTTTTT AAATAAAGAA 
CACCAGCAAG AAGCAGATAA AAAAAGACAA AGCAGAAGTG CTGCAAGTTA CCTTCATAAA 
AACCGGACTT GTGAGTGGAC ACGGGACATG TACAGAAACG AGTGCTTGGA ACTACGTAGT 
TTTTCAACCT GTCTTGCTCA CAGCATCACA AGAGATGTTA AAAAAGAGTT GATGGCATCA 
ACAGGTGGCT TGCCAAAATC CTTAACAGAT TCTTGTCTTT ATGAAAAAAA CGTATGTGAC 
GAAGATACTG AGACTCATGT CGGGCCAGAG TTTCCCAAGT CTCTTCAGCC TTCTTCACAG 
AATCACAGAT TTTCTCAGAG TACAGGCAGC TTGAGTGGGT GTGGCTATGG AGAGACTGTC 
GTTCAAGCTA TAGAGCAGTA TGCTAACAAA GTCGTGGGCG ACACTTTAGA GCTGGGTTTA 
GGATCTGCCA TCTTCCACGT GTCTGAAGCC ACAAAAGCAG TTGATAGGAT TACTTACGCA 
GAAAAGCTGT CACCTCTGGT GGGTCAGGCT TGCCGATACT GTGACCGTAA AGAGCTCCAT 
GACTGCACTA GAAATTCGTC TCCTCACTTT CCCAGACCGG ATTCACTTGC TGGTAGTAAG 
CCAGTTTCTC ATTCACAGCT TAGCAGCTTC TATCAGAAAT CTAGGATTTT TCATCTTGAT 
GTTCCTCAAA TTCACGTTGA TCTCGATAAG AAGACAGTGC TTGCTGAGAA GATAGTTGCT 
GAAGCGATTG AAAAGGCAGA GAGAGAGCTG AGCAGTACCA GCCAGGCAGC CGACAGTGGG 
ATCGGACAGG AAGGTGTCAG CTTTGCCGAG AGCCTCAGCA CAGAGATACT GGCGTCAGCA 
GTGGTCAGTG CTGGACAGGC CATCAGCAGT CCAAAAGAAA CAGAAGAGAT TCAGTCAGCT 
GAGTCTCTTG GTAGCCAGCA GATGCATCTC AGTATTGGTG AGGACAGTAC TGGGAGCTGG 
TCCAACTTAA GTTTTGAAGA TGAACACCAG GATGAAAGCA GTAGTTTTCA CCATCTAAGT 
GAAAGTGATG GGCCAGATGA TGATGAAGAG GAGCGTCAGA CTGATGTAGA AGGTTTGGAG 
CAAGATGGAA AGACTCTGCT AATTACAAAT ATTGACATGG AGCCATGCAC AGTAGACCCC 
CAGCTAAGGA TTATTCTTCA GTGGCTCGTT GCTTCTGAGG CAGAAGTTGC AGAACTTTAT 
TTTCATGACT CTACAAAGAA GGAGTTTATG CAACTTTCAA AACGGCTACA GGAAAAAGGG 
TGGAAGGTGG GAGACCTCCT GCAGGCTGTG CTTAAATACT ATGAGGAGGT GGAGAAGAAG 
TCCAGTGAGG AGAGATGCAA GTCCCTGTTT GATTGGCTCT TGGAAAATGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP11 ( XM_025263035.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025118820.1 Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X5, mRNA.

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CloneID	OBu47662
Clone ID Related Accession (Same CDS sequence)	XM_025263035.1
Accession Version	XM_025263035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	A-kinase anchor protein 11 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCT TCCAGTCTTT CAGAAACAAT CACATGAAAA CTAGAGCATC TGTCAGAAAA 
AGCTTCAATG AAGATGTGTT CCAGTCTGTG AAGTCTTTAT TGCAGAGCGA GAAGGAACTA 
TGCAGTGTAT CAGCCGAGGA CTGTTTGAAG CAGGATGAAC ATGCCAATTT GACTGAGGTC 
ACATTTCTAG GTTTTAATGA AGAAACAGAT GCTGCTCATA TACAGAGTCA ACATCCTGGA 
ATGCTTTGTG TCATGAGAGT GTCACCTACA TTACCAAGAC TTAAAATTGA TTTTATTTTT 
AGTCTCCTAA GTAAATATGC TACCGGCATA AGGTACACCC TGGACACATA TTTGCATCAC 
AAGCACCAAC TTGAGGCCAC CAATGAAGAC GACGATGATA CTAACCAGTC TGTATCTTCC 
ATCGAAGATG ACTTTGTCAC TGCTTTTGAG CACTTAGACG AGGAAGAGAC TTCAAAGCCA 
TATAATGATG GATTAAACAT AATTGCACTA AGGAGCCAGT GTGATGCTGC TTCACAGACT 
ATTTCTGGTC ACCATTTGGA TACCCATGAT TTCAGGGTTC TGGTTGGCTC TGAGCAGCAG 
AAGTCATTGG CTAAACCTTC AGCTTCTTTA ATAAACATTC TGGGACATAA AAAACTGCCT 
CCAGCGAGAA CTTCAGTCAC GACATCAGTC TCTGAACCAT GGATCCAGAG GAGTTTCTAT 
AGTTCCTTTG CTGCTTCAGA GAAAGGTAGT GATGCCCAGA AACCATTGTT CTCGTCTTCT 
CCTGCCTACT CGTCTGAATC AGAGTGCTCA AGTCCAAGTC CTGTTATTTT CTTGGATGAA 
GAAGGTTATC AAAAAAGTTT GAAAGCAAAA CTTGAGTTAC CTAAAATTCC TGTGATGAAA 
GATGATATTG AGGATTCAGA CTCAGAAGTG AGTGAGTTTT TTGATAGTTT TGATCAGTTT 
GATGAACTAG AACAAACGTT AGAGACTGCT TGTCCGTTTC TGAAAGATCT TGCTGTAGGG 
AAGCCATCGC AAAAGAAAGG GCACAAACAT GAAAAATTGT GCTCTGTAAC CACTACTATG 
AATCCTCAAA AATTCAAGTT TGATCGGCCA GCTCTCCCAG CTAATGTTAG GAAGCCAACA 
CCTCGTAAAC CAGAATCCCC TTATAGTAAC CTGTGTGATG CCCCAGATTC TCCCCGCCCC 
GTGAAGGCCT CAGGGGAAGA CAGTGGTTTG TTTAGTCCTA TTCGATCCTC TGCTTTCAGT 
CCTCTTGGAG GCTGTACTCC AGCTGAATGC TTTTGCCAAA CAGATATTGG TGGAGATAGG 
GGTCATGAAA ATCCTGATTC TCTTTATTAT ACCTATGAAG ATTATGCAAA TAGCCTTTCC 
TGTGAAGTTC TGGGCTCAGT TCTTCATAGC CAACATACTA ATGCAACATC CAACACTAAT 
AGTGTTAAAA AGGGAGAAAA TAAAATGGTA GCTTTTCAGC ATGGAAGCCT TGATCAAAAA 
AGTAAATCTA AAAATAAACC CATAATGATT GATAGCGTTC AGAAGTTTGC AGCAGATCTC 
GTGGAAAAAA GTTTTGGCAG TGCATTTAAA GACTTACAGA AAGGGGTCTC TTCATGTACC 
AATGCCTTAT GCCAGTTAGC CATCAAATTG ACGTCATCCA TTTTTCAAAT GGCATTTAAC 
GAACTGAGAA GGCAGTGTGC CTTTTCACTG AAAGAACGAG CCATTAGTGG CCTGGCTAGT 
TTTTTGGTGA GTGAGGCATT CTCAAATGCT TTAAAAGATC TACAATATGT AAAGAAACAG 
ATCTTCACTA ACACCGTCAC TAGATTTGCT GCAGATCTTG CTGAAGAGCT TGTTTTTGAA 
GGCATCATGG AAGTGTGTCA GTTCTCATAT CCTCCAACAC CTGCATCTCT GCAGGGTCGG 
TCATTTCACT GTGAAGACAG AGTGGTGAAG TCCTATGCAA GAGATCTGTC TGAATCTGTA 
ATACAGGAAG CATTCATTGA GCTGTCTCAA GTTGATGTTA CCTTTACCAC AAAGGCAGCA 
GTTAGTGTTT CCCCAGAAAA CATCAAGTTT GTGAGCACAG AAGATGTAGT GCCAGCAACA 
CAGACTTCTT TGTGTTCTTC TACTTCTAAC AGTCAAACAG TTACGGTGAC AAAACCAGTG 
CAGGAGTATA AAAAGGAATA CACGGTGCAG CAGGCCTTGT TCTGTACTTC AGGAATTGTT 
ACTTCAATAC CAGTGCCCTT GGCAGGAAGT GCCCTTCACC CATACCGTAT TTCTTCTAAT 
ACGTATCAGA CAAAGTCTCA TCCATCATGT GATGATAGTC ATTTGAATGG TGGTCCTACC 
CCAGCAAGTG TTGCCACAAA AAACAAAGAA GAGGAGCTAG CTTGTCTCAG GAATATTTGT 
TTACCTTCAG ACCACAACCC CGGTAGCCAG AATGAGGTGA AACCAACTAG TGATGTTGTT 
GAAACAAAAA GCTCTTCAAA GTTAACAAAT GATCCTGTCA TTATTGGCGA TTTTTCTGCC 
GCAATGGTGC ATACGATAGT AAATGAAACT ATAGATTCAC TGACATCTTC CAAAGTTGCA 
AAAACAGAAG AACACACAGA TTGTTTAACT CAAACAGGAA AGGGAAAAAC CCCTTCTTTC 
TACTGGGATC AAGCAACAGT GCCACACAGT GAGGCTAGAA AGAAGGACAT GTTCGCTGAT 
CGGTTATCTA AATCTATTAT CAAACATTCC ATAGATGAAA GCAAGTCAGT GATCCTAAAT 
GTAGATAAAA ATGTCCTCCC CAAGAAAGAC TTGCCTGTTT CTGGAGATGA GTCACAGTTG 
ACCTTGGAAG AGTTCCCCAA GTTTCTTGAA GCTCAAGATC ATTTAACTCA CTGTTCACTT 
TCAGAAGTAA AGGATTGTGT TCCAGAAGGT AAAGGTTCTG TGGCTCCTGG ATTTTCTTTG 
GAGACGCTAC CACCTTGTCT AGCAGCGGCA GGCCAGAAAG CTGATCTGAA GGAAATTGCT 
AAGGACAAAT CTGTGAAAAA GCAGAATTTG CATAATTCAG CCCTCGAGCC CTTGGCTTTT 
GGGCAGGAAA ACTCTTTTCC CCATTCACAT GCTTTCTCAT CCACAGTGCT CACCTGTGTA 
GATGGTTTGC ATGTGGAAGA TAAACAGAAA ATGAGAGATG GGAATGTAAT ACCTGACACC 
CCTCCATCAA CTCCTCTGGT TCCATCCCAG GCTCCTTCTG ATTGGGATAT CAAGAAGTTA 
ACCAAAAAGC TTAAGGGGGA GTTAGCAAAA GAGTTTGCAC CGGCCACACC ACCATCCACA 
CCTCACAACT CATCTGTAAG CAGTTTGTCT GAAAATGAAC AAAATGCAAT AGAAAAAGAA 
GAGTTCATGT TGAAGCTCAT GCGGTCTCTC TCAGAAGAAG TTGAAAGTAG TGACGGCGAA 
GAGCAGCAAG AGATGGACGT GAAATTGGAG CCAGCAGGGA AAAAAGTTCA GTTTGCAGAC 
GCATTAGCGA CGCACATCGT TTCTCTTGCA ACCGAAATGG CAGCTTCCCA TTTGGATAAT 
AAATTGATTC AAGAAGCCGG GGTGACAAGT CCTTGCTTAA ATGTGCAGAG TCAAAGAAGC 
CTATCACCTG CTTTTTTAAA TTGCTCAGAT GAAAGCTTAC AAGCATTATG CAGTTTTGCA 
GGTGATATGG CAGCAGAAGT CATTACAGAA GCTGAAAAAA TAGCAAAAGT TAGGAGCTGT 
ATGTTTTTCA GGAGGAAGAG GAACAGTTGT GTTGATGGTG ACCAAGATCG TAGATCCGAA 
GAGAAGTTGG ATGTAGAGGC TGTAGCGCAC CCGGGAGGAG TAGATCCGTT TGTTCTTCCT 
TTACCACCAA GTTCTTGTCT GTCAGGTCTA ACGTACAAGT ATCCCAGCTG TGAAAGTGTG 
ACAGATGAGT ATGCAGGTCA CATTATCCAA ATACTAAAAC AGGAAGGTGG CAATAGTGAG 
TTAATAATGG ACCAGTACGC CAACAGACTT GCCTATCGGT CTGTTAAGTC AGGATTGCAA 
GAAGCAGCTA AAACAGCCAA AGTGAAGTGC AGCTCCAAAA TACTCCCTGT GCAGAGCTCA 
CAGGTGAAAA CCAACGATGA ACTGTTACTG TTTTTAAATA AAGAACACCA GCAAGAAGCA 
GATAAAAAAA GACAAAGCAG AAGTGCTGCA AGTTACCTTC ATAAAAACCG GACTTGTGAG 
TGGACACGGG ACATGTACAG AAACGAGTGC TTGGAACTAC GTAGTTTTTC AACCTGTCTT 
GCTCACAGCA TCACAAGAGA TGTTAAAAAA GAGTTGATGG CATCAACAGG TGGCTTGCCA 
AAATCCTTAA CAGATTCTTG TCTTTATGAA AAAAACGTAT GTGACGAAGA TACTGAGACT 
CATGTCGGGC CAGAGTTTCC CAAGTCTCTT CAGCCTTCTT CACAGAATCA CAGATTTTCT 
CAGAGTACAG GCAGCTTGAG TGGGTGTGGC TATGGAGAGA CTGTCGTTCA AGCTATAGAG 
CAGTATGCTA ACAAAGTCGT GGGCGACACT TTAGAGCTGG GTTTAGGATC TGCCATCTTC 
CACGTGTCTG AAGCCACAAA AGCAGTTGAT AGGATTACTT ACGCAGAAAA GCTGTCACCT 
CTGGTGGGTC AGGCTTGCCG ATACTGTGAC CGTAAAGAGC TCCATGACTG CACTAGAAAT 
TCGTCTCCTC ACTTTCCCAG ACCGGATTCA CTTGCTGGTA GTAAGCCAGT TTCTCATTCA 
CAGCTTAGCA GCTTCTATCA GAAATCTAGG ATTTTTCATC TTGATGTTCC TCAAATTCAC 
GTTGATCTCG ATAAGAAGAC AGTGCTTGCT GAGAAGATAG TTGCTGAAGC GATTGAAAAG 
GCAGAGAGAG AGCTGAGCAG TACCAGCCAG GCAGCCGACA GTGGGATCGG ACAGGAAGGT 
GTCAGCTTTG CCGAGAGCCT CAGCACAGAG ATACTGGCGT CAGCAGTGGT CAGTGCTGGA 
CAGGCCATCA GCAGTCCAAA AGAAACAGAA GAGATTCAGT CAGCTGAGTC TCTTGGTAGC 
CAGCAGATGC ATCTCAGTAT TGGTGAGGAC AGTACTGGGA GCTGGTCCAA CTTAAGTTTT 
GAAGATGAAC ACCAGGATGA AAGCAGTAGT TTTCACCATC TAAGTGAAAG TAATGGTAAC 
AGCAGTAGCT GGAGCAGTCT TGGTTTAGAA GGAGATTTGT ATGAGGACAA TTTATCCTTT 
CCAACATCAG ACAGTGATGG GCCAGATGAT GATGAAGAGG AGCGTCAGAC TGATGTAGAA 
GGTTTGGAGC AAGATGGAAA GACTCTGCTA ATTACAAATA TTGACATGGA GCCATGCACA 
GTAGACCCCC AGCTAAGGAT TATTCTTCAG TGGCTCGTTG CTTCTGAGGC AGAAGTTGCA 
GAACTTTATT TTCATGACTC TACAAAGAAG GAGTTTATGC AACTTTCAAA ACGGCTACAG 
GAAAAAGGGT GGAAGGTGGG AGACCTCCTG CAGGCTGTGC TTAAATACTA TGAGGAGGTG 
GAGAAGAAGT CCAGTGAGGA GAGATGCAAG TCCCTGTTTG ATTGGCTCTT GGAAAATGCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025118820.1
CDS	178..5760
Translation	MATFQSFRNNHMKTRASVRKSFNEDVFQSVKSLLQSEKELCSVSAEDCLKQDEHANLTEVTFLGFNEETDAAHIQSQHPGMLCVMRVSPTLPRLKIDFIFSLLSKYATGIRYTLDTYLHHKHQLEATNEDDDDTNQSVSSIEDDFVTAFEHLDEEETSKPYNDGLNIIALRSQCDAASQTISGHHLDTHDFRVLVGSEQQKSLAKPSASLINILGHKKLPPARTSVTTSVSEPWIQRSFYSSFAASEKGSDAQKPLFSSSPAYSSESECSSPSPVIFLDEEGYQKSLKAKLELPKIPVMKDDIEDSDSEVSEFFDSFDQFDELEQTLETACPFLKDLAVGKPSQKKGHKHEKLCSVTTTMNPQKFKFDRPALPANVRKPTPRKPESPYSNLCDAPDSPRPVKASGEDSGLFSPIRSSAFSPLGGCTPAECFCQTDIGGDRGHENPDSLYYTYEDYANSLSCEVLGSVLHSQHTNATSNTNSVKKGENKMVAFQHGSLDQKSKSKNKPIMIDSVQKFAADLVEKSFGSAFKDLQKGVSSCTNALCQLAIKLTSSIFQMAFNELRRQCAFSLKERAISGLASFLVSEAFSNALKDLQYVKKQIFTNTVTRFAADLAEELVFEGIMEVCQFSYPPTPASLQGRSFHCEDRVVKSYARDLSESVIQEAFIELSQVDVTFTTKAAVSVSPENIKFVSTEDVVPATQTSLCSSTSNSQTVTVTKPVQEYKKEYTVQQALFCTSGIVTSIPVPLAGSALHPYRISSNTYQTKSHPSCDDSHLNGGPTPASVATKNKEEELACLRNICLPSDHNPGSQNEVKPTSDVVETKSSSKLTNDPVIIGDFSAAMVHTIVNETIDSLTSSKVAKTEEHTDCLTQTGKGKTPSFYWDQATVPHSEARKKDMFADRLSKSIIKHSIDESKSVILNVDKNVLPKKDLPVSGDESQLTLEEFPKFLEAQDHLTHCSLSEVKDCVPEGKGSVAPGFSLETLPPCLAAAGQKADLKEIAKDKSVKKQNLHNSALEPLAFGQENSFPHSHAFSSTVLTCVDGLHVEDKQKMRDGNVIPDTPPSTPLVPSQAPSDWDIKKLTKKLKGELAKEFAPATPPSTPHNSSVSSLSENEQNAIEKEEFMLKLMRSLSEEVESSDGEEQQEMDVKLEPAGKKVQFADALATHIVSLATEMAASHLDNKLIQEAGVTSPCLNVQSQRSLSPAFLNCSDESLQALCSFAGDMAAEVITEAEKIAKVRSCMFFRRKRNSCVDGDQDRRSEEKLDVEAVAHPGGVDPFVLPLPPSSCLSGLTYKYPSCESVTDEYAGHIIQILKQEGGNSELIMDQYANRLAYRSVKSGLQEAAKTAKVKCSSKILPVQSSQVKTNDELLLFLNKEHQQEADKKRQSRSAASYLHKNRTCEWTRDMYRNECLELRSFSTCLAHSITRDVKKELMASTGGLPKSLTDSCLYEKNVCDEDTETHVGPEFPKSLQPSSQNHRFSQSTGSLSGCGYGETVVQAIEQYANKVVGDTLELGLGSAIFHVSEATKAVDRITYAEKLSPLVGQACRYCDRKELHDCTRNSSPHFPRPDSLAGSKPVSHSQLSSFYQKSRIFHLDVPQIHVDLDKKTVLAEKIVAEAIEKAERELSSTSQAADSGIGQEGVSFAESLSTEILASAVVSAGQAISSPKETEEIQSAESLGSQQMHLSIGEDSTGSWSNLSFEDEHQDESSSFHHLSESNGNSSSWSSLGLEGDLYEDNLSFPTSDSDGPDDDEEERQTDVEGLEQDGKTLLITNIDMEPCTVDPQLRIILQWLVASEAEVAELYFHDSTKKEFMQLSKRLQEKGWKVGDLLQAVLKYYEEVEKKSSEERCKSLFDWLLENA

Target ORF information:

RefSeq Version	XM_025263035.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025263035.1

ORF Insert Sequence:

ATGGCGACCT TCCAGTCTTT CAGAAACAAT CACATGAAAA CTAGAGCATC TGTCAGAAAA 
AGCTTCAATG AAGATGTGTT CCAGTCTGTG AAGTCTTTAT TGCAGAGCGA GAAGGAACTA 
TGCAGTGTAT CAGCCGAGGA CTGTTTGAAG CAGGATGAAC ATGCCAATTT GACTGAGGTC 
ACATTTCTAG GTTTTAATGA AGAAACAGAT GCTGCTCATA TACAGAGTCA ACATCCTGGA 
ATGCTTTGTG TCATGAGAGT GTCACCTACA TTACCAAGAC TTAAAATTGA TTTTATTTTT 
AGTCTCCTAA GTAAATATGC TACCGGCATA AGGTACACCC TGGACACATA TTTGCATCAC 
AAGCACCAAC TTGAGGCCAC CAATGAAGAC GACGATGATA CTAACCAGTC TGTATCTTCC 
ATCGAAGATG ACTTTGTCAC TGCTTTTGAG CACTTAGACG AGGAAGAGAC TTCAAAGCCA 
TATAATGATG GATTAAACAT AATTGCACTA AGGAGCCAGT GTGATGCTGC TTCACAGACT 
ATTTCTGGTC ACCATTTGGA TACCCATGAT TTCAGGGTTC TGGTTGGCTC TGAGCAGCAG 
AAGTCATTGG CTAAACCTTC AGCTTCTTTA ATAAACATTC TGGGACATAA AAAACTGCCT 
CCAGCGAGAA CTTCAGTCAC GACATCAGTC TCTGAACCAT GGATCCAGAG GAGTTTCTAT 
AGTTCCTTTG CTGCTTCAGA GAAAGGTAGT GATGCCCAGA AACCATTGTT CTCGTCTTCT 
CCTGCCTACT CGTCTGAATC AGAGTGCTCA AGTCCAAGTC CTGTTATTTT CTTGGATGAA 
GAAGGTTATC AAAAAAGTTT GAAAGCAAAA CTTGAGTTAC CTAAAATTCC TGTGATGAAA 
GATGATATTG AGGATTCAGA CTCAGAAGTG AGTGAGTTTT TTGATAGTTT TGATCAGTTT 
GATGAACTAG AACAAACGTT AGAGACTGCT TGTCCGTTTC TGAAAGATCT TGCTGTAGGG 
AAGCCATCGC AAAAGAAAGG GCACAAACAT GAAAAATTGT GCTCTGTAAC CACTACTATG 
AATCCTCAAA AATTCAAGTT TGATCGGCCA GCTCTCCCAG CTAATGTTAG GAAGCCAACA 
CCTCGTAAAC CAGAATCCCC TTATAGTAAC CTGTGTGATG CCCCAGATTC TCCCCGCCCC 
GTGAAGGCCT CAGGGGAAGA CAGTGGTTTG TTTAGTCCTA TTCGATCCTC TGCTTTCAGT 
CCTCTTGGAG GCTGTACTCC AGCTGAATGC TTTTGCCAAA CAGATATTGG TGGAGATAGG 
GGTCATGAAA ATCCTGATTC TCTTTATTAT ACCTATGAAG ATTATGCAAA TAGCCTTTCC 
TGTGAAGTTC TGGGCTCAGT TCTTCATAGC CAACATACTA ATGCAACATC CAACACTAAT 
AGTGTTAAAA AGGGAGAAAA TAAAATGGTA GCTTTTCAGC ATGGAAGCCT TGATCAAAAA 
AGTAAATCTA AAAATAAACC CATAATGATT GATAGCGTTC AGAAGTTTGC AGCAGATCTC 
GTGGAAAAAA GTTTTGGCAG TGCATTTAAA GACTTACAGA AAGGGGTCTC TTCATGTACC 
AATGCCTTAT GCCAGTTAGC CATCAAATTG ACGTCATCCA TTTTTCAAAT GGCATTTAAC 
GAACTGAGAA GGCAGTGTGC CTTTTCACTG AAAGAACGAG CCATTAGTGG CCTGGCTAGT 
TTTTTGGTGA GTGAGGCATT CTCAAATGCT TTAAAAGATC TACAATATGT AAAGAAACAG 
ATCTTCACTA ACACCGTCAC TAGATTTGCT GCAGATCTTG CTGAAGAGCT TGTTTTTGAA 
GGCATCATGG AAGTGTGTCA GTTCTCATAT CCTCCAACAC CTGCATCTCT GCAGGGTCGG 
TCATTTCACT GTGAAGACAG AGTGGTGAAG TCCTATGCAA GAGATCTGTC TGAATCTGTA 
ATACAGGAAG CATTCATTGA GCTGTCTCAA GTTGATGTTA CCTTTACCAC AAAGGCAGCA 
GTTAGTGTTT CCCCAGAAAA CATCAAGTTT GTGAGCACAG AAGATGTAGT GCCAGCAACA 
CAGACTTCTT TGTGTTCTTC TACTTCTAAC AGTCAAACAG TTACGGTGAC AAAACCAGTG 
CAGGAGTATA AAAAGGAATA CACGGTGCAG CAGGCCTTGT TCTGTACTTC AGGAATTGTT 
ACTTCAATAC CAGTGCCCTT GGCAGGAAGT GCCCTTCACC CATACCGTAT TTCTTCTAAT 
ACGTATCAGA CAAAGTCTCA TCCATCATGT GATGATAGTC ATTTGAATGG TGGTCCTACC 
CCAGCAAGTG TTGCCACAAA AAACAAAGAA GAGGAGCTAG CTTGTCTCAG GAATATTTGT 
TTACCTTCAG ACCACAACCC CGGTAGCCAG AATGAGGTGA AACCAACTAG TGATGTTGTT 
GAAACAAAAA GCTCTTCAAA GTTAACAAAT GATCCTGTCA TTATTGGCGA TTTTTCTGCC 
GCAATGGTGC ATACGATAGT AAATGAAACT ATAGATTCAC TGACATCTTC CAAAGTTGCA 
AAAACAGAAG AACACACAGA TTGTTTAACT CAAACAGGAA AGGGAAAAAC CCCTTCTTTC 
TACTGGGATC AAGCAACAGT GCCACACAGT GAGGCTAGAA AGAAGGACAT GTTCGCTGAT 
CGGTTATCTA AATCTATTAT CAAACATTCC ATAGATGAAA GCAAGTCAGT GATCCTAAAT 
GTAGATAAAA ATGTCCTCCC CAAGAAAGAC TTGCCTGTTT CTGGAGATGA GTCACAGTTG 
ACCTTGGAAG AGTTCCCCAA GTTTCTTGAA GCTCAAGATC ATTTAACTCA CTGTTCACTT 
TCAGAAGTAA AGGATTGTGT TCCAGAAGGT AAAGGTTCTG TGGCTCCTGG ATTTTCTTTG 
GAGACGCTAC CACCTTGTCT AGCAGCGGCA GGCCAGAAAG CTGATCTGAA GGAAATTGCT 
AAGGACAAAT CTGTGAAAAA GCAGAATTTG CATAATTCAG CCCTCGAGCC CTTGGCTTTT 
GGGCAGGAAA ACTCTTTTCC CCATTCACAT GCTTTCTCAT CCACAGTGCT CACCTGTGTA 
GATGGTTTGC ATGTGGAAGA TAAACAGAAA ATGAGAGATG GGAATGTAAT ACCTGACACC 
CCTCCATCAA CTCCTCTGGT TCCATCCCAG GCTCCTTCTG ATTGGGATAT CAAGAAGTTA 
ACCAAAAAGC TTAAGGGGGA GTTAGCAAAA GAGTTTGCAC CGGCCACACC ACCATCCACA 
CCTCACAACT CATCTGTAAG CAGTTTGTCT GAAAATGAAC AAAATGCAAT AGAAAAAGAA 
GAGTTCATGT TGAAGCTCAT GCGGTCTCTC TCAGAAGAAG TTGAAAGTAG TGACGGCGAA 
GAGCAGCAAG AGATGGACGT GAAATTGGAG CCAGCAGGGA AAAAAGTTCA GTTTGCAGAC 
GCATTAGCGA CGCACATCGT TTCTCTTGCA ACCGAAATGG CAGCTTCCCA TTTGGATAAT 
AAATTGATTC AAGAAGCCGG GGTGACAAGT CCTTGCTTAA ATGTGCAGAG TCAAAGAAGC 
CTATCACCTG CTTTTTTAAA TTGCTCAGAT GAAAGCTTAC AAGCATTATG CAGTTTTGCA 
GGTGATATGG CAGCAGAAGT CATTACAGAA GCTGAAAAAA TAGCAAAAGT TAGGAGCTGT 
ATGTTTTTCA GGAGGAAGAG GAACAGTTGT GTTGATGGTG ACCAAGATCG TAGATCCGAA 
GAGAAGTTGG ATGTAGAGGC TGTAGCGCAC CCGGGAGGAG TAGATCCGTT TGTTCTTCCT 
TTACCACCAA GTTCTTGTCT GTCAGGTCTA ACGTACAAGT ATCCCAGCTG TGAAAGTGTG 
ACAGATGAGT ATGCAGGTCA CATTATCCAA ATACTAAAAC AGGAAGGTGG CAATAGTGAG 
TTAATAATGG ACCAGTACGC CAACAGACTT GCCTATCGGT CTGTTAAGTC AGGATTGCAA 
GAAGCAGCTA AAACAGCCAA AGTGAAGTGC AGCTCCAAAA TACTCCCTGT GCAGAGCTCA 
CAGGTGAAAA CCAACGATGA ACTGTTACTG TTTTTAAATA AAGAACACCA GCAAGAAGCA 
GATAAAAAAA GACAAAGCAG AAGTGCTGCA AGTTACCTTC ATAAAAACCG GACTTGTGAG 
TGGACACGGG ACATGTACAG AAACGAGTGC TTGGAACTAC GTAGTTTTTC AACCTGTCTT 
GCTCACAGCA TCACAAGAGA TGTTAAAAAA GAGTTGATGG CATCAACAGG TGGCTTGCCA 
AAATCCTTAA CAGATTCTTG TCTTTATGAA AAAAACGTAT GTGACGAAGA TACTGAGACT 
CATGTCGGGC CAGAGTTTCC CAAGTCTCTT CAGCCTTCTT CACAGAATCA CAGATTTTCT 
CAGAGTACAG GCAGCTTGAG TGGGTGTGGC TATGGAGAGA CTGTCGTTCA AGCTATAGAG 
CAGTATGCTA ACAAAGTCGT GGGCGACACT TTAGAGCTGG GTTTAGGATC TGCCATCTTC 
CACGTGTCTG AAGCCACAAA AGCAGTTGAT AGGATTACTT ACGCAGAAAA GCTGTCACCT 
CTGGTGGGTC AGGCTTGCCG ATACTGTGAC CGTAAAGAGC TCCATGACTG CACTAGAAAT 
TCGTCTCCTC ACTTTCCCAG ACCGGATTCA CTTGCTGGTA GTAAGCCAGT TTCTCATTCA 
CAGCTTAGCA GCTTCTATCA GAAATCTAGG ATTTTTCATC TTGATGTTCC TCAAATTCAC 
GTTGATCTCG ATAAGAAGAC AGTGCTTGCT GAGAAGATAG TTGCTGAAGC GATTGAAAAG 
GCAGAGAGAG AGCTGAGCAG TACCAGCCAG GCAGCCGACA GTGGGATCGG ACAGGAAGGT 
GTCAGCTTTG CCGAGAGCCT CAGCACAGAG ATACTGGCGT CAGCAGTGGT CAGTGCTGGA 
CAGGCCATCA GCAGTCCAAA AGAAACAGAA GAGATTCAGT CAGCTGAGTC TCTTGGTAGC 
CAGCAGATGC ATCTCAGTAT TGGTGAGGAC AGTACTGGGA GCTGGTCCAA CTTAAGTTTT 
GAAGATGAAC ACCAGGATGA AAGCAGTAGT TTTCACCATC TAAGTGAAAG TAATGGTAAC 
AGCAGTAGCT GGAGCAGTCT TGGTTTAGAA GGAGATTTGT ATGAGGACAA TTTATCCTTT 
CCAACATCAG ACAGTGATGG GCCAGATGAT GATGAAGAGG AGCGTCAGAC TGATGTAGAA 
GGTTTGGAGC AAGATGGAAA GACTCTGCTA ATTACAAATA TTGACATGGA GCCATGCACA 
GTAGACCCCC AGCTAAGGAT TATTCTTCAG TGGCTCGTTG CTTCTGAGGC AGAAGTTGCA 
GAACTTTATT TTCATGACTC TACAAAGAAG GAGTTTATGC AACTTTCAAA ACGGCTACAG 
GAAAAAGGGT GGAAGGTGGG AGACCTCCTG CAGGCTGTGC TTAAATACTA TGAGGAGGTG 
GAGAAGAAGT CCAGTGAGGA GAGATGCAAG TCCCTGTTTG ATTGGCTCTT GGAAAATGCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP11 ( XM_025263036.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025118821.1 Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X6, mRNA.

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CloneID	OBu47663
Clone ID Related Accession (Same CDS sequence)	XM_025263036.1
Accession Version	XM_025263036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	A-kinase anchor protein 11 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCT TCCAGTCTTT CAGAAACAAT CACATGAAAA CTAGAGCATC TGTCAGAAAA 
AGCTTCAATG AAGATGTGTT CCAGTCTGTG AAGTCTTTAT TGCAGAGCGA GAAGGAACTA 
TGCAGTGTAT CAGCCGAGGA CTGTTTGAAG CAGGATGAAC ATGCCAATTT GACTGAGGTC 
ACATTTCTAG GTTTTAATGA AGAAACAGAT GCTGCTCATA TACAGGATTT GGCTGCAGTT 
TCTTTGGAAC TCCCAGATCT TCTGAACTCA CTGCACTTCT GCAGTCTAAA TGAAAATGAA 
ATTATTTGTA TGAAGGATAT AAATAAATCA TCAGATATAA ACAGTGATCC TCTAAACCAG 
AGTCAACATC CTGGAATGCT TTGTGTCATG AGAGTGTCAC CTACATTACC AAGACTTAAA 
ATTGATTTTA TTTTTAGTCT CCTAAGTAAA TATGCTACCG GCATAAGGTA CACCCTGGAC 
ACATATTTGC ATCACAAGCA CCAACTTGAG GCCACCAATG AAGACGACGA TGATACTAAC 
CAGTCTGTAT CTTCCATCGA AGATGACTTT GTCACTGCTT TTGAGCACTT AGACGAGGAA 
GAGACTTCAA AGCCATATAA TGATGGATTA AACATAATTG CACTAAGGAG CCAGTGTGAT 
GCTGCTTCAC AGACTATTTC TGGTCACCAT TTGGATACCC ATGATTTCAG GGTTCTGGTT 
GGCTCTGAGC AGCAGAAGTC ATTGGCTAAA CCTTCAGCTT CTTTAATAAA CATTCTGGGA 
CATAAAAAAC TGCCTCCAGC GAGAACTTCA GTCACGACAT CAGTCTCTGA ACCATGGATC 
CAGAGGAGTT TCTATAGTTC CTTTGCTGCT TCAGAGAAAG GTAGTGATGC CCAGAAACCA 
TTGTTCTCGT CTTCTCCTGC CTACTCGTCT GAATCAGAGT GCTCAAGTCC AAGTCCTGTT 
ATTTTCTTGG ATGAAGAAGG TTATCAAAAA AGTTTGAAAG CAAAACTTGA GTTACCTAAA 
ATTCCTGTGA TGAAAGATGA TATTGAGGAT TCAGACTCAG AAGTGAGTGA GTTTTTTGAT 
AGTTTTGATC AGTTTGATGA ACTAGAACAA ACGTTAGAGA CTGCTTGTCC GTTTCTGAAA 
GATCTTGCTG TAGGGAAGCC ATCGCAAAAG AAAGGGCACA AACATGAAAA ATTGTGCTCT 
GTAACCACTA CTATGAATCC TCAAAAATTC AAGTTTGATC GGCCAGCTCT CCCAGCTAAT 
GTTAGGAAGC CAACACCTCG TAAACCAGAA TCCCCTTATA GTAACCTGTG TGATGCCCCA 
GATTCTCCCC GCCCCGTGAA GGCCTCAGGG GAAGACAGTG GTTTGTTTAG TCCTATTCGA 
TCCTCTGCTT TCAGTCCTCT TGGAGGCTGT ACTCCAGCTG AATGCTTTTG CCAAACAGAT 
ATTGGTGGAG ATAGGGGTCA TGAAAATCCT GATTCTCTTT ATTATACCTA TGAAGATTAT 
GCAAATAGCC TTTCCTGTGA AGTTCTGGGC TCAGTTCTTC ATAGCCAACA TACTAATGCA 
ACATCCAACA CTAATAGTGT TAAAAAGGGA GAAAATAAAA TGGTAGCTTT TCAGCATGGA 
AGCCTTGATC AAAAAAGTAA ATCTAAAAAT AAACCCATAA TGATTGATAG CGTTCAGAAG 
TTTGCAGCAG ATCTCGTGGA AAAAAGTTTT GGCAGTGCAT TTAAAGACTT ACAGAAAGGG 
GTCTCTTCAT GTACCAATGC CTTATGCCAG TTAGCCATCA AATTGACGTC ATCCATTTTT 
CAAATGGCAT TTAACGAACT GAGAAGGCAG TGTGCCTTTT CACTGAAAGA ACGAGCCATT 
AGTGGCCTGG CTAGTTTTTT GGTGAGTGAG GCATTCTCAA ATGCTTTAAA AGATCTACAA 
TATGTAAAGA AACAGATCTT CACTAACACC GTCACTAGAT TTGCTGCAGA TCTTGCTGAA 
GAGCTTGTTT TTGAAGGCAT CATGGAAGTG TGTCAGTTCT CATATCCTCC AACACCTGCA 
TCTCTGCAGG GTCGGTCATT TCACTGTGAA GACAGAGTGG TGAAGTCCTA TGCAAGAGAT 
CTGTCTGAAT CTGTAATACA GGAAGCATTC ATTGAGCTGT CTCAAGTTGA TGTTACCTTT 
ACCACAAAGG CAGCAGTTAG TGTTTCCCCA GAAAACATCA AGTTTGTGAG CACAGAAGAT 
GTAGTGCCAG CAACACAGAC TTCTTTGTGT TCTTCTACTT CTAACAGTCA AACAGTTACG 
GTGACAAAAC CAGTGCAGGA GTATAAAAAG GAATACACGG TGCAGCAGGC CTTGTTCTGT 
ACTTCAGGAA TTGTTACTTC AATACCAGTG CCCTTGGCAG GAAGTGCCCT TCACCCATAC 
CGTATTTCTT CTAATACGTA TCAGACAAAG TCTCATCCAT CATGTGATGA TAGTCATTTG 
AATGGTGGTC CTACCCCAGC AAGTGTTGCC ACAAAAAACA AAGAAGAGGA GCTAGCTTGT 
CTCAGGAATA TTTGTTTACC TTCAGACCAC AACCCCGGTA GCCAGAATGA GGTGAAACCA 
ACTAGTGATG TTGTTGAAAC AAAAAGCTCT TCAAAGTTAA CAAATGATCC TGTCATTATT 
GGCGATTTTT CTGCCGCAAT GGTGCATACG ATAGTAAATG AAACTATAGA TTCACTGACA 
TCTTCCAAAG TTGCAAAAAC AGAAGAACAC ACAGATTGTT TAACTCAAAC AGGAAAGGGA 
AAAACCCCTT CTTTCTACTG GGATCAAGCA ACAGTGCCAC ACAGTGAGGC TAGAAAGAAG 
GACATGTTCG CTGATCGGTT ATCTAAATCT ATTATCAAAC ATTCCATAGA TGAAAGCAAG 
TCAGTGATCC TAAATGTAGA TAAAAATGTC CTCCCCAAGA AAGACTTGCC TGTTTCTGGA 
GATGAGTCAC AGTTGACCTT GGAAGAGTTC CCCAAGTTTC TTGAAGCTCA AGATCATTTA 
ACTCACTGTT CACTTTCAGA AGTAAAGGAT TGTGTTCCAG AAGGTAAAGG TTCTGTGGCT 
CCTGGATTTT CTTTGGAGAC GCTACCACCT TGTCTAGCAG CGGCAGGCCA GAAAGCTGAT 
CTGAAGGAAA TTGCTAAGGA CAAATCTGTG AAAAAGCAGA ATTTGCATAA TTCAGCCCTC 
GAGCCCTTGG CTTTTGGGCA GGAAAACTCT TTTCCCCATT CACATGCTTT CTCATCCACA 
GTGCTCACCT GTGTAGATGG TTTGCATGTG GAAGATAAAC AGAAAATGAG AGATGGGAAT 
GTAATACCTG ACACCCCTCC ATCAACTCCT CTGGTTCCAT CCCAGGCTCC TTCTGATTGG 
GATATCAAGA AGTTAACCAA AAAGCTTAAG GGGGAGTTAG CAAAAGAGTT TGCACCGGCC 
ACACCACCAT CCACACCTCA CAACTCATCT GTAAGCAGTT TGTCTGAAAA TGAACAAAAT 
GCAATAGAAA AAGAAGAGTT CATGTTGAAG CTCATGCGGT CTCTCTCAGA AGAAGTTGAA 
AGTAGTGACG GCGAAGAGCA GCAAGAGATG GACGTGAAAT TGGAGCCAGC AGGGAAAAAA 
GTTCAGTTTG CAGACGCATT AGCGACGCAC ATCGTTTCTC TTGCAACCGA AATGGCAGCT 
TCCCATTTGG ATAATAAATT GATTCAAGAA GCCGGGGTGA CAAGTCCTTG CTTAAATGTG 
CAGAGTCAAA GAAGCCTATC ACCTGCTTTT TTAAATTGCT CAGATGAAAG CTTACAAGCA 
TTATGCAGTT TTGCAGGTGA TATGGCAGCA GAAGTCATTA CAGAAGCTGA AAAAATAGCA 
AAAGTTAGGA GCTGTATGTT TTTCAGGAGG AAGAGGAACA GTTGTGTTGA TGGTGACCAA 
GATCGTAGAT CCGAAGAGAA GTTGGATGTA GAGGCTGTAG CGCACCCGGG AGGAGTAGAT 
CCGTTTGTTC TTCCTTTACC ACCAAGTTCT TGTCTGTCAG GTCTAACGTA CAAGTATCCC 
AGCTGTGAAA GTGTGACAGA TGAGTATGCA GGTCACATTA TCCAAATACT AAAACAGGAA 
GGTGGCAATA GTGAGTTAAT AATGGACCAG TACGCCAACA GACTTGCCTA TCGGTCTGTT 
AAGTCAGGAT TGCAAGAAGC AGCTAAAACA GCCAAAGTGA AGTGCAGCTC CAAAATACTC 
CCTGTGCAGA GCTCACAGGT GAAAACCAAC GATGAACTGT TACTGTTTTT AAATAAAGAA 
CACCAGCAAG AAGCAGATAA AAAAAGACAA AGCAGAAGTG CTGCAAGTTA CCTTCATAAA 
AACCGGACTT GTGAGTGGAC ACGGGACATG TACAGAAACG AGTGCTTGGA ACTACGTAGT 
TTTTCAACCT GTCTTGCTCA CAGCATCACA AGAGATGTTA AAAAAGAGTT GATGGCATCA 
ACAGGTGGCT TGCCAAAATC CTTAACAGAT TCTTGTCTTT ATGAAAAAAA CGTATGTGAC 
GAAGATACTG AGACTCATGT CGGGCCAGAG TTTCCCAAGT CTCTTCAGCC TTCTTCACAG 
AATCACAGAT TTTCTCAGAG TACAGGCAGC TTGAGTGGGT GTGGCTATGG AGAGACTGTC 
GTTCAAGCTA TAGAGCAGTA TGCTAACAAA GTCGTGGGCG ACACTTTAGA GCTGGGTTTA 
GGATCTGCCA TCTTCCACGT GTCTGAAGCC ACAAAAGCAG TTGATAGGAT TACTTACGCA 
GAAAAGCTGT CACCTCTGGT GGGTCAGGCT TGCCGATACT GTGACCGTAA AGAGCTCCAT 
GACTGCACTA GAAATTCGTC TCCTCACTTT CCCAGACCGG ATTCACTTGC TGGTAGTAAG 
CCAGTTTCTC ATTCACAGCT TAGCAGCTTC TATCAGAAAT CTAGGATTTT TCATCTTGAT 
GTTCCTCAAA TTCACGTTGA TCTCGATAAG AAGACAGTGC TTGCTGAGAA GATAGTTGCT 
GAAGCGATTG AAAAGGCAGA GAGAGAGCTG AGCAGTACCA GCCAGGCAGC CGACAGTGGG 
ATCGGACAGG AAGGTGTCAG CTTTGCCGAG AGCCTCAGCA CAGAGATACT GGCGTCAGCA 
GTGGTCAGTG CTGGACAGGC CATCAGCAGT GATGGGCCAG ATGATGATGA AGAGGAGCGT 
CAGACTGATG TAGAAGGTTT GGAGCAAGAT GGAAAGACTC TGCTAATTAC AAATATTGAC 
ATGGAGCCAT GCACAGTAGA CCCCCAGCTA AGGATTATTC TTCAGTGGCT CGTTGCTTCT 
GAGGCAGAAG TTGCAGAACT TTATTTTCAT GACTCTACAA AGAAGGAGTT TATGCAACTT 
TCAAAACGGC TACAGGAAAA AGGGTGGAAG GTGGGAGACC TCCTGCAGGC TGTGCTTAAA 
TACTATGAGG AGGTGGAGAA GAAGTCCAGT GAGGAGAGAT GCAAGTCCCT GTTTGATTGG 
CTCTTGGAAA ATGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025118821.1
CDS	178..5655
Translation	MATFQSFRNNHMKTRASVRKSFNEDVFQSVKSLLQSEKELCSVSAEDCLKQDEHANLTEVTFLGFNEETDAAHIQDLAAVSLELPDLLNSLHFCSLNENEIICMKDINKSSDINSDPLNQSQHPGMLCVMRVSPTLPRLKIDFIFSLLSKYATGIRYTLDTYLHHKHQLEATNEDDDDTNQSVSSIEDDFVTAFEHLDEEETSKPYNDGLNIIALRSQCDAASQTISGHHLDTHDFRVLVGSEQQKSLAKPSASLINILGHKKLPPARTSVTTSVSEPWIQRSFYSSFAASEKGSDAQKPLFSSSPAYSSESECSSPSPVIFLDEEGYQKSLKAKLELPKIPVMKDDIEDSDSEVSEFFDSFDQFDELEQTLETACPFLKDLAVGKPSQKKGHKHEKLCSVTTTMNPQKFKFDRPALPANVRKPTPRKPESPYSNLCDAPDSPRPVKASGEDSGLFSPIRSSAFSPLGGCTPAECFCQTDIGGDRGHENPDSLYYTYEDYANSLSCEVLGSVLHSQHTNATSNTNSVKKGENKMVAFQHGSLDQKSKSKNKPIMIDSVQKFAADLVEKSFGSAFKDLQKGVSSCTNALCQLAIKLTSSIFQMAFNELRRQCAFSLKERAISGLASFLVSEAFSNALKDLQYVKKQIFTNTVTRFAADLAEELVFEGIMEVCQFSYPPTPASLQGRSFHCEDRVVKSYARDLSESVIQEAFIELSQVDVTFTTKAAVSVSPENIKFVSTEDVVPATQTSLCSSTSNSQTVTVTKPVQEYKKEYTVQQALFCTSGIVTSIPVPLAGSALHPYRISSNTYQTKSHPSCDDSHLNGGPTPASVATKNKEEELACLRNICLPSDHNPGSQNEVKPTSDVVETKSSSKLTNDPVIIGDFSAAMVHTIVNETIDSLTSSKVAKTEEHTDCLTQTGKGKTPSFYWDQATVPHSEARKKDMFADRLSKSIIKHSIDESKSVILNVDKNVLPKKDLPVSGDESQLTLEEFPKFLEAQDHLTHCSLSEVKDCVPEGKGSVAPGFSLETLPPCLAAAGQKADLKEIAKDKSVKKQNLHNSALEPLAFGQENSFPHSHAFSSTVLTCVDGLHVEDKQKMRDGNVIPDTPPSTPLVPSQAPSDWDIKKLTKKLKGELAKEFAPATPPSTPHNSSVSSLSENEQNAIEKEEFMLKLMRSLSEEVESSDGEEQQEMDVKLEPAGKKVQFADALATHIVSLATEMAASHLDNKLIQEAGVTSPCLNVQSQRSLSPAFLNCSDESLQALCSFAGDMAAEVITEAEKIAKVRSCMFFRRKRNSCVDGDQDRRSEEKLDVEAVAHPGGVDPFVLPLPPSSCLSGLTYKYPSCESVTDEYAGHIIQILKQEGGNSELIMDQYANRLAYRSVKSGLQEAAKTAKVKCSSKILPVQSSQVKTNDELLLFLNKEHQQEADKKRQSRSAASYLHKNRTCEWTRDMYRNECLELRSFSTCLAHSITRDVKKELMASTGGLPKSLTDSCLYEKNVCDEDTETHVGPEFPKSLQPSSQNHRFSQSTGSLSGCGYGETVVQAIEQYANKVVGDTLELGLGSAIFHVSEATKAVDRITYAEKLSPLVGQACRYCDRKELHDCTRNSSPHFPRPDSLAGSKPVSHSQLSSFYQKSRIFHLDVPQIHVDLDKKTVLAEKIVAEAIEKAERELSSTSQAADSGIGQEGVSFAESLSTEILASAVVSAGQAISSDGPDDDEEERQTDVEGLEQDGKTLLITNIDMEPCTVDPQLRIILQWLVASEAEVAELYFHDSTKKEFMQLSKRLQEKGWKVGDLLQAVLKYYEEVEKKSSEERCKSLFDWLLENA

Target ORF information:

RefSeq Version	XM_025263036.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis A-kinase anchoring protein 11 (AKAP11), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025263036.1

ORF Insert Sequence:

ATGGCGACCT TCCAGTCTTT CAGAAACAAT CACATGAAAA CTAGAGCATC TGTCAGAAAA 
AGCTTCAATG AAGATGTGTT CCAGTCTGTG AAGTCTTTAT TGCAGAGCGA GAAGGAACTA 
TGCAGTGTAT CAGCCGAGGA CTGTTTGAAG CAGGATGAAC ATGCCAATTT GACTGAGGTC 
ACATTTCTAG GTTTTAATGA AGAAACAGAT GCTGCTCATA TACAGGATTT GGCTGCAGTT 
TCTTTGGAAC TCCCAGATCT TCTGAACTCA CTGCACTTCT GCAGTCTAAA TGAAAATGAA 
ATTATTTGTA TGAAGGATAT AAATAAATCA TCAGATATAA ACAGTGATCC TCTAAACCAG 
AGTCAACATC CTGGAATGCT TTGTGTCATG AGAGTGTCAC CTACATTACC AAGACTTAAA 
ATTGATTTTA TTTTTAGTCT CCTAAGTAAA TATGCTACCG GCATAAGGTA CACCCTGGAC 
ACATATTTGC ATCACAAGCA CCAACTTGAG GCCACCAATG AAGACGACGA TGATACTAAC 
CAGTCTGTAT CTTCCATCGA AGATGACTTT GTCACTGCTT TTGAGCACTT AGACGAGGAA 
GAGACTTCAA AGCCATATAA TGATGGATTA AACATAATTG CACTAAGGAG CCAGTGTGAT 
GCTGCTTCAC AGACTATTTC TGGTCACCAT TTGGATACCC ATGATTTCAG GGTTCTGGTT 
GGCTCTGAGC AGCAGAAGTC ATTGGCTAAA CCTTCAGCTT CTTTAATAAA CATTCTGGGA 
CATAAAAAAC TGCCTCCAGC GAGAACTTCA GTCACGACAT CAGTCTCTGA ACCATGGATC 
CAGAGGAGTT TCTATAGTTC CTTTGCTGCT TCAGAGAAAG GTAGTGATGC CCAGAAACCA 
TTGTTCTCGT CTTCTCCTGC CTACTCGTCT GAATCAGAGT GCTCAAGTCC AAGTCCTGTT 
ATTTTCTTGG ATGAAGAAGG TTATCAAAAA AGTTTGAAAG CAAAACTTGA GTTACCTAAA 
ATTCCTGTGA TGAAAGATGA TATTGAGGAT TCAGACTCAG AAGTGAGTGA GTTTTTTGAT 
AGTTTTGATC AGTTTGATGA ACTAGAACAA ACGTTAGAGA CTGCTTGTCC GTTTCTGAAA 
GATCTTGCTG TAGGGAAGCC ATCGCAAAAG AAAGGGCACA AACATGAAAA ATTGTGCTCT 
GTAACCACTA CTATGAATCC TCAAAAATTC AAGTTTGATC GGCCAGCTCT CCCAGCTAAT 
GTTAGGAAGC CAACACCTCG TAAACCAGAA TCCCCTTATA GTAACCTGTG TGATGCCCCA 
GATTCTCCCC GCCCCGTGAA GGCCTCAGGG GAAGACAGTG GTTTGTTTAG TCCTATTCGA 
TCCTCTGCTT TCAGTCCTCT TGGAGGCTGT ACTCCAGCTG AATGCTTTTG CCAAACAGAT 
ATTGGTGGAG ATAGGGGTCA TGAAAATCCT GATTCTCTTT ATTATACCTA TGAAGATTAT 
GCAAATAGCC TTTCCTGTGA AGTTCTGGGC TCAGTTCTTC ATAGCCAACA TACTAATGCA 
ACATCCAACA CTAATAGTGT TAAAAAGGGA GAAAATAAAA TGGTAGCTTT TCAGCATGGA 
AGCCTTGATC AAAAAAGTAA ATCTAAAAAT AAACCCATAA TGATTGATAG CGTTCAGAAG 
TTTGCAGCAG ATCTCGTGGA AAAAAGTTTT GGCAGTGCAT TTAAAGACTT ACAGAAAGGG 
GTCTCTTCAT GTACCAATGC CTTATGCCAG TTAGCCATCA AATTGACGTC ATCCATTTTT 
CAAATGGCAT TTAACGAACT GAGAAGGCAG TGTGCCTTTT CACTGAAAGA ACGAGCCATT 
AGTGGCCTGG CTAGTTTTTT GGTGAGTGAG GCATTCTCAA ATGCTTTAAA AGATCTACAA 
TATGTAAAGA AACAGATCTT CACTAACACC GTCACTAGAT TTGCTGCAGA TCTTGCTGAA 
GAGCTTGTTT TTGAAGGCAT CATGGAAGTG TGTCAGTTCT CATATCCTCC AACACCTGCA 
TCTCTGCAGG GTCGGTCATT TCACTGTGAA GACAGAGTGG TGAAGTCCTA TGCAAGAGAT 
CTGTCTGAAT CTGTAATACA GGAAGCATTC ATTGAGCTGT CTCAAGTTGA TGTTACCTTT 
ACCACAAAGG CAGCAGTTAG TGTTTCCCCA GAAAACATCA AGTTTGTGAG CACAGAAGAT 
GTAGTGCCAG CAACACAGAC TTCTTTGTGT TCTTCTACTT CTAACAGTCA AACAGTTACG 
GTGACAAAAC CAGTGCAGGA GTATAAAAAG GAATACACGG TGCAGCAGGC CTTGTTCTGT 
ACTTCAGGAA TTGTTACTTC AATACCAGTG CCCTTGGCAG GAAGTGCCCT TCACCCATAC 
CGTATTTCTT CTAATACGTA TCAGACAAAG TCTCATCCAT CATGTGATGA TAGTCATTTG 
AATGGTGGTC CTACCCCAGC AAGTGTTGCC ACAAAAAACA AAGAAGAGGA GCTAGCTTGT 
CTCAGGAATA TTTGTTTACC TTCAGACCAC AACCCCGGTA GCCAGAATGA GGTGAAACCA 
ACTAGTGATG TTGTTGAAAC AAAAAGCTCT TCAAAGTTAA CAAATGATCC TGTCATTATT 
GGCGATTTTT CTGCCGCAAT GGTGCATACG ATAGTAAATG AAACTATAGA TTCACTGACA 
TCTTCCAAAG TTGCAAAAAC AGAAGAACAC ACAGATTGTT TAACTCAAAC AGGAAAGGGA 
AAAACCCCTT CTTTCTACTG GGATCAAGCA ACAGTGCCAC ACAGTGAGGC TAGAAAGAAG 
GACATGTTCG CTGATCGGTT ATCTAAATCT ATTATCAAAC ATTCCATAGA TGAAAGCAAG 
TCAGTGATCC TAAATGTAGA TAAAAATGTC CTCCCCAAGA AAGACTTGCC TGTTTCTGGA 
GATGAGTCAC AGTTGACCTT GGAAGAGTTC CCCAAGTTTC TTGAAGCTCA AGATCATTTA 
ACTCACTGTT CACTTTCAGA AGTAAAGGAT TGTGTTCCAG AAGGTAAAGG TTCTGTGGCT 
CCTGGATTTT CTTTGGAGAC GCTACCACCT TGTCTAGCAG CGGCAGGCCA GAAAGCTGAT 
CTGAAGGAAA TTGCTAAGGA CAAATCTGTG AAAAAGCAGA ATTTGCATAA TTCAGCCCTC 
GAGCCCTTGG CTTTTGGGCA GGAAAACTCT TTTCCCCATT CACATGCTTT CTCATCCACA 
GTGCTCACCT GTGTAGATGG TTTGCATGTG GAAGATAAAC AGAAAATGAG AGATGGGAAT 
GTAATACCTG ACACCCCTCC ATCAACTCCT CTGGTTCCAT CCCAGGCTCC TTCTGATTGG 
GATATCAAGA AGTTAACCAA AAAGCTTAAG GGGGAGTTAG CAAAAGAGTT TGCACCGGCC 
ACACCACCAT CCACACCTCA CAACTCATCT GTAAGCAGTT TGTCTGAAAA TGAACAAAAT 
GCAATAGAAA AAGAAGAGTT CATGTTGAAG CTCATGCGGT CTCTCTCAGA AGAAGTTGAA 
AGTAGTGACG GCGAAGAGCA GCAAGAGATG GACGTGAAAT TGGAGCCAGC AGGGAAAAAA 
GTTCAGTTTG CAGACGCATT AGCGACGCAC ATCGTTTCTC TTGCAACCGA AATGGCAGCT 
TCCCATTTGG ATAATAAATT GATTCAAGAA GCCGGGGTGA CAAGTCCTTG CTTAAATGTG 
CAGAGTCAAA GAAGCCTATC ACCTGCTTTT TTAAATTGCT CAGATGAAAG CTTACAAGCA 
TTATGCAGTT TTGCAGGTGA TATGGCAGCA GAAGTCATTA CAGAAGCTGA AAAAATAGCA 
AAAGTTAGGA GCTGTATGTT TTTCAGGAGG AAGAGGAACA GTTGTGTTGA TGGTGACCAA 
GATCGTAGAT CCGAAGAGAA GTTGGATGTA GAGGCTGTAG CGCACCCGGG AGGAGTAGAT 
CCGTTTGTTC TTCCTTTACC ACCAAGTTCT TGTCTGTCAG GTCTAACGTA CAAGTATCCC 
AGCTGTGAAA GTGTGACAGA TGAGTATGCA GGTCACATTA TCCAAATACT AAAACAGGAA 
GGTGGCAATA GTGAGTTAAT AATGGACCAG TACGCCAACA GACTTGCCTA TCGGTCTGTT 
AAGTCAGGAT TGCAAGAAGC AGCTAAAACA GCCAAAGTGA AGTGCAGCTC CAAAATACTC 
CCTGTGCAGA GCTCACAGGT GAAAACCAAC GATGAACTGT TACTGTTTTT AAATAAAGAA 
CACCAGCAAG AAGCAGATAA AAAAAGACAA AGCAGAAGTG CTGCAAGTTA CCTTCATAAA 
AACCGGACTT GTGAGTGGAC ACGGGACATG TACAGAAACG AGTGCTTGGA ACTACGTAGT 
TTTTCAACCT GTCTTGCTCA CAGCATCACA AGAGATGTTA AAAAAGAGTT GATGGCATCA 
ACAGGTGGCT TGCCAAAATC CTTAACAGAT TCTTGTCTTT ATGAAAAAAA CGTATGTGAC 
GAAGATACTG AGACTCATGT CGGGCCAGAG TTTCCCAAGT CTCTTCAGCC TTCTTCACAG 
AATCACAGAT TTTCTCAGAG TACAGGCAGC TTGAGTGGGT GTGGCTATGG AGAGACTGTC 
GTTCAAGCTA TAGAGCAGTA TGCTAACAAA GTCGTGGGCG ACACTTTAGA GCTGGGTTTA 
GGATCTGCCA TCTTCCACGT GTCTGAAGCC ACAAAAGCAG TTGATAGGAT TACTTACGCA 
GAAAAGCTGT CACCTCTGGT GGGTCAGGCT TGCCGATACT GTGACCGTAA AGAGCTCCAT 
GACTGCACTA GAAATTCGTC TCCTCACTTT CCCAGACCGG ATTCACTTGC TGGTAGTAAG 
CCAGTTTCTC ATTCACAGCT TAGCAGCTTC TATCAGAAAT CTAGGATTTT TCATCTTGAT 
GTTCCTCAAA TTCACGTTGA TCTCGATAAG AAGACAGTGC TTGCTGAGAA GATAGTTGCT 
GAAGCGATTG AAAAGGCAGA GAGAGAGCTG AGCAGTACCA GCCAGGCAGC CGACAGTGGG 
ATCGGACAGG AAGGTGTCAG CTTTGCCGAG AGCCTCAGCA CAGAGATACT GGCGTCAGCA 
GTGGTCAGTG CTGGACAGGC CATCAGCAGT GATGGGCCAG ATGATGATGA AGAGGAGCGT 
CAGACTGATG TAGAAGGTTT GGAGCAAGAT GGAAAGACTC TGCTAATTAC AAATATTGAC 
ATGGAGCCAT GCACAGTAGA CCCCCAGCTA AGGATTATTC TTCAGTGGCT CGTTGCTTCT 
GAGGCAGAAG TTGCAGAACT TTATTTTCAT GACTCTACAA AGAAGGAGTT TATGCAACTT 
TCAAAACGGC TACAGGAAAA AGGGTGGAAG GTGGGAGACC TCCTGCAGGC TGTGCTTAAA 
TACTATGAGG AGGTGGAGAA GAAGTCCAGT GAGGAGAGAT GCAAGTCCCT GTTTGATTGG 
CTCTTGGAAA ATGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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AKAP11 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

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