LOC102389575 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following LOC102389575 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC102389575 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu21224 XM_006065752.1
Latest version!
Bubalus bubalis NIMA-related kinase 5 (NEK5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu21225 XM_006065753.1
Latest version!
Bubalus bubalis NIMA-related kinase 5 (NEK5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu21226 XM_006065754.1
Latest version!
Bubalus bubalis NIMA-related kinase 5 (NEK5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBu21227 XM_006065755.1
Latest version!
Bubalus bubalis NIMA-related kinase 5 (NEK5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBu47604 XM_006065752.2
Latest version!
Bubalus bubalis serine/threonine-protein kinase Nek5 (LOC102389575), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu47605 XM_006065753.2
Latest version!
Bubalus bubalis serine/threonine-protein kinase Nek5 (LOC102389575), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu47606 XM_025262949.1
Latest version!
Bubalus bubalis serine/threonine-protein kinase Nek5 (LOC102389575), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu47607 XM_025262950.1
Latest version!
Bubalus bubalis serine/threonine-protein kinase Nek5 (LOC102389575), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu47608 XM_006065754.2
Latest version!
Bubalus bubalis serine/threonine-protein kinase Nek5 (LOC102389575), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu21224
    Clone ID Related Accession (Same CDS sequence) XM_006065752.1
    Accession Version XM_006065752.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2514bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product serine/threonine-protein kinase Nek5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785175.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATCAAT ATGACATGAT TAAAGCCATT GGGGAAGGTG CCTTTGGGAA AGCGTACTTG 
    GCCCAAAGGA AATCGGATAG CGAGCACTGT GTTATAAAAG AGATCAATTT TGAAAAGATG
    CCCATCCAAG AAAAAGAAGC TTCCAAGAAA GAAGTCATTC TTCTGGCAAA GATGAAACAT
    CCCAACATTG TAACCTTCTT CAATTCATTT CAAGAGAACA ACAGACTGTT TATTGTGATG
    GAGTATTGTG ATGGCGGGGA TCTCATGAAA AGGATCAGAA GACAACGGGG AGTGTTATTT
    AGTGAAGATC AGATTCTGAG TTGGTTTGTA CAGATTTCTC TAGGACTGAA ACATATTCAC
    GACAGGAAGG TTTTACACAG GGACATAAAA ACACAGAACA TTTTTCTTAG CAAGAACGGA
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGATACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG TTTCAGGATA TTGAGAGAGA CTTAAAACAA
    ATGAAACCTC AGAACATAAA GGAAAGTAAA AGTTCGGAAC AAAAACATAA AGCTCAGAGA
    GGGCTAAAAT TTGAAATTAA TTTAAATGAA TGTATTTCTG ATGAAAACAC CCTCCAAGAG
    GAAGAGGTAG TGGATATACT GAATGAAACT TTGATCTCTG AGGATGGCAT GAAGTTCATG
    GAATATAAAT GTATGAAGGA GCAGGAAGAT TATACAGACC AAGCATTTGA AACACTCTGT
    TGCCCAGAAG CAGAGTTGTG CATTCCGGAT GCCACTGCTG CACAGCACAG GAGACAGTGG
    GCCCCAGGAG CTCCTCAGAC TCTGCTGCAA ATCATGGCCT CGGCCGATGT CACCTCCACC
    TGCTCCACCT GCTCCACCAT GCCTGAGGAT GGCCGAGTGA TTGAGATTGA AGGCGGTCCA
    GAAACCAGGA GACGGTGGCA GCGAGAAGCA CCGGGAACTT TACTGAGTGT TTTGGCAGCA
    GCACAGCTAA CCAGGAGCTC GCTTTCTGCC AACGATGGAG AATGTGTGGG ACCACTGAAA
    GAAGATAAGG GGAAGGTGGA AGTGGCCTCT GACATTGAAA TAGATGCAGA ACAACCAGAT
    CCAAGATCTG ATGACGATGA TACAAATTTT GAAGGATCTG AAGATGAATT GAGAAATGAA
    GTAGTAGAAT CTCTGGAAAA ACTGGCTACT TTCAATGAGG CAGAAAAAAG AGAAGAGGTT
    TCTAGTTCTT CTAAAGATGC TGGAAACTTA GAAGAAACAG AGGGGATAGG CATGCAGAAA
    TATTCAGAAT TAAATGAAGG TTTGGAGAGT ATTTTTATTC CATCTGATGA CAAAATTTGT
    ATTGGTGATG AAGATCAAGG AGCATCAACC AACAGTCAAA ATATTCAACT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006065814.1
    CDS104..2617
    Translation

    Target ORF information:

    RefSeq Version XM_006065752.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NIMA-related kinase 5 (NEK5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006065752.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATCAAT ATGACATGAT TAAAGCCATT GGGGAAGGTG CCTTTGGGAA AGCGTACTTG 
    GCCCAAAGGA AATCGGATAG CGAGCACTGT GTTATAAAAG AGATCAATTT TGAAAAGATG
    CCCATCCAAG AAAAAGAAGC TTCCAAGAAA GAAGTCATTC TTCTGGCAAA GATGAAACAT
    CCCAACATTG TAACCTTCTT CAATTCATTT CAAGAGAACA ACAGACTGTT TATTGTGATG
    GAGTATTGTG ATGGCGGGGA TCTCATGAAA AGGATCAGAA GACAACGGGG AGTGTTATTT
    AGTGAAGATC AGATTCTGAG TTGGTTTGTA CAGATTTCTC TAGGACTGAA ACATATTCAC
    GACAGGAAGG TTTTACACAG GGACATAAAA ACACAGAACA TTTTTCTTAG CAAGAACGGA
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGATACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG TTTCAGGATA TTGAGAGAGA CTTAAAACAA
    ATGAAACCTC AGAACATAAA GGAAAGTAAA AGTTCGGAAC AAAAACATAA AGCTCAGAGA
    GGGCTAAAAT TTGAAATTAA TTTAAATGAA TGTATTTCTG ATGAAAACAC CCTCCAAGAG
    GAAGAGGTAG TGGATATACT GAATGAAACT TTGATCTCTG AGGATGGCAT GAAGTTCATG
    GAATATAAAT GTATGAAGGA GCAGGAAGAT TATACAGACC AAGCATTTGA AACACTCTGT
    TGCCCAGAAG CAGAGTTGTG CATTCCGGAT GCCACTGCTG CACAGCACAG GAGACAGTGG
    GCCCCAGGAG CTCCTCAGAC TCTGCTGCAA ATCATGGCCT CGGCCGATGT CACCTCCACC
    TGCTCCACCT GCTCCACCAT GCCTGAGGAT GGCCGAGTGA TTGAGATTGA AGGCGGTCCA
    GAAACCAGGA GACGGTGGCA GCGAGAAGCA CCGGGAACTT TACTGAGTGT TTTGGCAGCA
    GCACAGCTAA CCAGGAGCTC GCTTTCTGCC AACGATGGAG AATGTGTGGG ACCACTGAAA
    GAAGATAAGG GGAAGGTGGA AGTGGCCTCT GACATTGAAA TAGATGCAGA ACAACCAGAT
    CCAAGATCTG ATGACGATGA TACAAATTTT GAAGGATCTG AAGATGAATT GAGAAATGAA
    GTAGTAGAAT CTCTGGAAAA ACTGGCTACT TTCAATGAGG CAGAAAAAAG AGAAGAGGTT
    TCTAGTTCTT CTAAAGATGC TGGAAACTTA GAAGAAACAG AGGGGATAGG CATGCAGAAA
    TATTCAGAAT TAAATGAAGG TTTGGAGAGT ATTTTTATTC CATCTGATGA CAAAATTTGT
    ATTGGTGATG AAGATCAAGG AGCATCAACC AACAGTCAAA ATATTCAACT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu21225
    Clone ID Related Accession (Same CDS sequence) XM_006065753.1
    Accession Version XM_006065753.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product serine/threonine-protein kinase Nek5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785175.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATCAAT ATGACATGAT TAAAGCCATT GGGGAAGGTG CCTTTGGGAA AGCGTACTTG 
    GCCCAAAGGA AATCGGATAG CGAGCACTGT GTTATAAAAG AGATCAATTT TGAAAAGATG
    CCCATCCAAG AAAAAGAAGC TTCCAAGAAA GAAGTCATTC TTCTGGCAAA GATGAAACAT
    CCCAACATTG TAACCTTCTT CAATTCATTT CAAGAGAACA ACAGACTGTT TATTGTGATG
    GAGTATTGTG ATGGCGGGGA TCTCATGAAA AGGATCAGAA GACAACGGGG AGTGTTATTT
    AGTGAAGATC AGATTCTGAG TTGGTTTGTA CAGATTTCTC TAGGACTGAA ACATATTCAC
    GACAGGAAGG TTTTACACAG GGACATAAAA ACACAGAACA TTTTTCTTAG CAAGAACGGA
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGATACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG GATATTGAGA GAGACTTAAA ACAAATGAAA
    CCTCAGAACA TAAAGGAAAG TAAAAGTTCG GAACAAAAAC ATAAAGCTCA GAGAGGGCTA
    AAATTTGAAA TTAATTTAAA TGAATGTATT TCTGATGAAA ACACCCTCCA AGAGGAAGAG
    GTAGTGGATA TACTGAATGA AACTTTGATC TCTGAGGATG GCATGAAGTT CATGGAATAT
    AAATGTATGA AGGAGCAGGA AGATTATACA GACCAAGCAT TTGAAACACT CTGTTGCCCA
    GAAGCAGAGT TGTGCATTCC GGATGCCACT GCTGCACAGC ACAGGAGACA GTGGGCCCCA
    GGAGCTCCTC AGACTCTGCT GCAAATCATG GCCTCGGCCG ATGTCACCTC CACCTGCTCC
    ACCTGCTCCA CCATGCCTGA GGATGGCCGA GTGATTGAGA TTGAAGGCGG TCCAGAAACC
    AGGAGACGGT GGCAGCGAGA AGCACCGGGA ACTTTACTGA GTGTTTTGGC AGCAGCACAG
    CTAACCAGGA GCTCGCTTTC TGCCAACGAT GGAGAATGTG TGGGACCACT GAAAGAAGAT
    AAGGGGAAGG TGGAAGTGGC CTCTGACATT GAAATAGATG CAGAACAACC AGATCCAAGA
    TCTGATGACG ATGATACAAA TTTTGAAGGA TCTGAAGATG AATTGAGAAA TGAAGTAGTA
    GAATCTCTGG AAAAACTGGC TACTTTCAAT GAGGCAGAAA AAAGAGAAGA GGTTTCTAGT
    TCTTCTAAAG ATGCTGGAAA CTTAGAAGAA ACAGAGGGGA TAGGCATGCA GAAATATTCA
    GAATTAAATG AAGGTTTGGA GAGTATTTTT ATTCCATCTG ATGACAAAAT TTGTATTGGT
    GATGAAGATC AAGGAGCATC AACCAACAGT CAAAATATTC AACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006065815.1
    CDS104..2611
    Translation

    Target ORF information:

    RefSeq Version XM_006065753.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NIMA-related kinase 5 (NEK5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006065753.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATCAAT ATGACATGAT TAAAGCCATT GGGGAAGGTG CCTTTGGGAA AGCGTACTTG 
    GCCCAAAGGA AATCGGATAG CGAGCACTGT GTTATAAAAG AGATCAATTT TGAAAAGATG
    CCCATCCAAG AAAAAGAAGC TTCCAAGAAA GAAGTCATTC TTCTGGCAAA GATGAAACAT
    CCCAACATTG TAACCTTCTT CAATTCATTT CAAGAGAACA ACAGACTGTT TATTGTGATG
    GAGTATTGTG ATGGCGGGGA TCTCATGAAA AGGATCAGAA GACAACGGGG AGTGTTATTT
    AGTGAAGATC AGATTCTGAG TTGGTTTGTA CAGATTTCTC TAGGACTGAA ACATATTCAC
    GACAGGAAGG TTTTACACAG GGACATAAAA ACACAGAACA TTTTTCTTAG CAAGAACGGA
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGATACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG GATATTGAGA GAGACTTAAA ACAAATGAAA
    CCTCAGAACA TAAAGGAAAG TAAAAGTTCG GAACAAAAAC ATAAAGCTCA GAGAGGGCTA
    AAATTTGAAA TTAATTTAAA TGAATGTATT TCTGATGAAA ACACCCTCCA AGAGGAAGAG
    GTAGTGGATA TACTGAATGA AACTTTGATC TCTGAGGATG GCATGAAGTT CATGGAATAT
    AAATGTATGA AGGAGCAGGA AGATTATACA GACCAAGCAT TTGAAACACT CTGTTGCCCA
    GAAGCAGAGT TGTGCATTCC GGATGCCACT GCTGCACAGC ACAGGAGACA GTGGGCCCCA
    GGAGCTCCTC AGACTCTGCT GCAAATCATG GCCTCGGCCG ATGTCACCTC CACCTGCTCC
    ACCTGCTCCA CCATGCCTGA GGATGGCCGA GTGATTGAGA TTGAAGGCGG TCCAGAAACC
    AGGAGACGGT GGCAGCGAGA AGCACCGGGA ACTTTACTGA GTGTTTTGGC AGCAGCACAG
    CTAACCAGGA GCTCGCTTTC TGCCAACGAT GGAGAATGTG TGGGACCACT GAAAGAAGAT
    AAGGGGAAGG TGGAAGTGGC CTCTGACATT GAAATAGATG CAGAACAACC AGATCCAAGA
    TCTGATGACG ATGATACAAA TTTTGAAGGA TCTGAAGATG AATTGAGAAA TGAAGTAGTA
    GAATCTCTGG AAAAACTGGC TACTTTCAAT GAGGCAGAAA AAAGAGAAGA GGTTTCTAGT
    TCTTCTAAAG ATGCTGGAAA CTTAGAAGAA ACAGAGGGGA TAGGCATGCA GAAATATTCA
    GAATTAAATG AAGGTTTGGA GAGTATTTTT ATTCCATCTG ATGACAAAAT TTGTATTGGT
    GATGAAGATC AAGGAGCATC AACCAACAGT CAAAATATTC AACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu21226
    Clone ID Related Accession (Same CDS sequence) XM_006065754.1
    Accession Version XM_006065754.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2358bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product serine/threonine-protein kinase Nek5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785175.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGATCAAT ATGACATGAT TAAAGCCATT GGGGAAGGTG CCTTTGGGAA AGCGTACTTG 
    GCCCAAAGGA AATCGGATAG CGAGCACTGT GTTATAAAAG AGATCAATTT TGAAAAGATG
    CCCATCCAAG AAAAAGAAGC TTCCAAGAAA GAAGTCATTC TTCTGGCAAA GATGAAACAT
    CCCAACATTG TAACCTTCTT CAATTCATTT CAAGAGAACA ACAGACTGTT TATTGTGATG
    GAGTATTGTG ATGGCGGGGA TCTCATGAAA AGGATCAGAA GACAACGGGG AGTGTTATTT
    AGTGAAGATC AGATTCTGAG TTGGTTTGTA CAGATTTCTC TAGGACTGAA ACATATTCAC
    GACAGGAAGG TTTTACACAG GGACATAAAA ACACAGAACA TTTTTCTTAG CAAGAACGGA
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGATACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG GTAGTGGATA TACTGAATGA AACTTTGATC
    TCTGAGGATG GCATGAAGTT CATGGAATAT AAATGTATGA AGGAGCAGGA AGATTATACA
    GACCAAGCAT TTGAAACACT CTGTTGCCCA GAAGCAGAGT TGTGCATTCC GGATGCCACT
    GCTGCACAGC ACAGGAGACA GTGGGCCCCA GGAGCTCCTC AGACTCTGCT GCAAATCATG
    GCCTCGGCCG ATGTCACCTC CACCTGCTCC ACCTGCTCCA CCATGCCTGA GGATGGCCGA
    GTGATTGAGA TTGAAGGCGG TCCAGAAACC AGGAGACGGT GGCAGCGAGA AGCACCGGGA
    ACTTTACTGA GTGTTTTGGC AGCAGCACAG CTAACCAGGA GCTCGCTTTC TGCCAACGAT
    GGAGAATGTG TGGGACCACT GAAAGAAGAT AAGGGGAAGG TGGAAGTGGC CTCTGACATT
    GAAATAGATG CAGAACAACC AGATCCAAGA TCTGATGACG ATGATACAAA TTTTGAAGGA
    TCTGAAGATG AATTGAGAAA TGAAGTAGTA GAATCTCTGG AAAAACTGGC TACTTTCAAT
    GAGGCAGAAA AAAGAGAAGA GGTTTCTAGT TCTTCTAAAG ATGCTGGAAA CTTAGAAGAA
    ACAGAGGGGA TAGGCATGCA GAAATATTCA GAATTAAATG AAGGTTTGGA GAGTATTTTT
    ATTCCATCTG ATGACAAAAT TTGTATTGGT GATGAAGATC AAGGAGCATC AACCAACAGT
    CAAAATATTC AACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006065816.1
    CDS104..2461
    Translation

    Target ORF information:

    RefSeq Version XM_006065754.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NIMA-related kinase 5 (NEK5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006065754.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGATCAAT ATGACATGAT TAAAGCCATT GGGGAAGGTG CCTTTGGGAA AGCGTACTTG 
    GCCCAAAGGA AATCGGATAG CGAGCACTGT GTTATAAAAG AGATCAATTT TGAAAAGATG
    CCCATCCAAG AAAAAGAAGC TTCCAAGAAA GAAGTCATTC TTCTGGCAAA GATGAAACAT
    CCCAACATTG TAACCTTCTT CAATTCATTT CAAGAGAACA ACAGACTGTT TATTGTGATG
    GAGTATTGTG ATGGCGGGGA TCTCATGAAA AGGATCAGAA GACAACGGGG AGTGTTATTT
    AGTGAAGATC AGATTCTGAG TTGGTTTGTA CAGATTTCTC TAGGACTGAA ACATATTCAC
    GACAGGAAGG TTTTACACAG GGACATAAAA ACACAGAACA TTTTTCTTAG CAAGAACGGA
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGATACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG GTAGTGGATA TACTGAATGA AACTTTGATC
    TCTGAGGATG GCATGAAGTT CATGGAATAT AAATGTATGA AGGAGCAGGA AGATTATACA
    GACCAAGCAT TTGAAACACT CTGTTGCCCA GAAGCAGAGT TGTGCATTCC GGATGCCACT
    GCTGCACAGC ACAGGAGACA GTGGGCCCCA GGAGCTCCTC AGACTCTGCT GCAAATCATG
    GCCTCGGCCG ATGTCACCTC CACCTGCTCC ACCTGCTCCA CCATGCCTGA GGATGGCCGA
    GTGATTGAGA TTGAAGGCGG TCCAGAAACC AGGAGACGGT GGCAGCGAGA AGCACCGGGA
    ACTTTACTGA GTGTTTTGGC AGCAGCACAG CTAACCAGGA GCTCGCTTTC TGCCAACGAT
    GGAGAATGTG TGGGACCACT GAAAGAAGAT AAGGGGAAGG TGGAAGTGGC CTCTGACATT
    GAAATAGATG CAGAACAACC AGATCCAAGA TCTGATGACG ATGATACAAA TTTTGAAGGA
    TCTGAAGATG AATTGAGAAA TGAAGTAGTA GAATCTCTGG AAAAACTGGC TACTTTCAAT
    GAGGCAGAAA AAAGAGAAGA GGTTTCTAGT TCTTCTAAAG ATGCTGGAAA CTTAGAAGAA
    ACAGAGGGGA TAGGCATGCA GAAATATTCA GAATTAAATG AAGGTTTGGA GAGTATTTTT
    ATTCCATCTG ATGACAAAAT TTGTATTGGT GATGAAGATC AAGGAGCATC AACCAACAGT
    CAAAATATTC AACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu21227
    Clone ID Related Accession (Same CDS sequence) XM_006065755.1
    Accession Version XM_006065755.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2094bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product serine/threonine-protein kinase Nek5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785175.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT 
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGATACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG TTTCAGGATA TTGAGAGAGA CTTAAAACAA
    ATGAAACCTC AGAACATAAA GGAAAGTAAA AGTTCGGAAC AAAAACATAA AGCTCAGAGA
    GGGCTAAAAT TTGAAATTAA TTTAAATGAA TGTATTTCTG ATGAAAACAC CCTCCAAGAG
    GAAGAGGTAG TGGATATACT GAATGAAACT TTGATCTCTG AGGATGGCAT GAAGTTCATG
    GAATATAAAT GTATGAAGGA GCAGGAAGAT TATACAGACC AAGCATTTGA AACACTCTGT
    TGCCCAGAAG CAGAGTTGTG CATTCCGGAT GCCACTGCTG CACAGCACAG GAGACAGTGG
    GCCCCAGGAG CTCCTCAGAC TCTGCTGCAA ATCATGGCCT CGGCCGATGT CACCTCCACC
    TGCTCCACCT GCTCCACCAT GCCTGAGGAT GGCCGAGTGA TTGAGATTGA AGGCGGTCCA
    GAAACCAGGA GACGGTGGCA GCGAGAAGCA CCGGGAACTT TACTGAGTGT TTTGGCAGCA
    GCACAGCTAA CCAGGAGCTC GCTTTCTGCC AACGATGGAG AATGTGTGGG ACCACTGAAA
    GAAGATAAGG GGAAGGTGGA AGTGGCCTCT GACATTGAAA TAGATGCAGA ACAACCAGAT
    CCAAGATCTG ATGACGATGA TACAAATTTT GAAGGATCTG AAGATGAATT GAGAAATGAA
    GTAGTAGAAT CTCTGGAAAA ACTGGCTACT TTCAATGAGG CAGAAAAAAG AGAAGAGGTT
    TCTAGTTCTT CTAAAGATGC TGGAAACTTA GAAGAAACAG AGGGGATAGG CATGCAGAAA
    TATTCAGAAT TAAATGAAGG TTTGGAGAGT ATTTTTATTC CATCTGATGA CAAAATTTGT
    ATTGGTGATG AAGATCAAGG AGCATCAACC AACAGTCAAA ATATTCAACT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006065817.1
    CDS42..2135
    Translation

    Target ORF information:

    RefSeq Version XM_006065755.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NIMA-related kinase 5 (NEK5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006065755.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT 
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGATACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG TTTCAGGATA TTGAGAGAGA CTTAAAACAA
    ATGAAACCTC AGAACATAAA GGAAAGTAAA AGTTCGGAAC AAAAACATAA AGCTCAGAGA
    GGGCTAAAAT TTGAAATTAA TTTAAATGAA TGTATTTCTG ATGAAAACAC CCTCCAAGAG
    GAAGAGGTAG TGGATATACT GAATGAAACT TTGATCTCTG AGGATGGCAT GAAGTTCATG
    GAATATAAAT GTATGAAGGA GCAGGAAGAT TATACAGACC AAGCATTTGA AACACTCTGT
    TGCCCAGAAG CAGAGTTGTG CATTCCGGAT GCCACTGCTG CACAGCACAG GAGACAGTGG
    GCCCCAGGAG CTCCTCAGAC TCTGCTGCAA ATCATGGCCT CGGCCGATGT CACCTCCACC
    TGCTCCACCT GCTCCACCAT GCCTGAGGAT GGCCGAGTGA TTGAGATTGA AGGCGGTCCA
    GAAACCAGGA GACGGTGGCA GCGAGAAGCA CCGGGAACTT TACTGAGTGT TTTGGCAGCA
    GCACAGCTAA CCAGGAGCTC GCTTTCTGCC AACGATGGAG AATGTGTGGG ACCACTGAAA
    GAAGATAAGG GGAAGGTGGA AGTGGCCTCT GACATTGAAA TAGATGCAGA ACAACCAGAT
    CCAAGATCTG ATGACGATGA TACAAATTTT GAAGGATCTG AAGATGAATT GAGAAATGAA
    GTAGTAGAAT CTCTGGAAAA ACTGGCTACT TTCAATGAGG CAGAAAAAAG AGAAGAGGTT
    TCTAGTTCTT CTAAAGATGC TGGAAACTTA GAAGAAACAG AGGGGATAGG CATGCAGAAA
    TATTCAGAAT TAAATGAAGG TTTGGAGAGT ATTTTTATTC CATCTGATGA CAAAATTTGT
    ATTGGTGATG AAGATCAAGG AGCATCAACC AACAGTCAAA ATATTCAACT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu47604
    Clone ID Related Accession (Same CDS sequence) XM_006065752.2
    Accession Version XM_006065752.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2514bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product serine/threonine-protein kinase Nek5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006065752.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATCAAT ATGACATGAT TAAAGCCATT GGGGAAGGTG CCTTTGGGAA AGCGTACTTG 
    GCCCAAAGGA AATCGGATAG CGAGCACTGT GTTATAAAAG AGATCAATTT TGAAAAGATG
    CCCATCCAAG AAAAAGAAGC TTCCAAGAAA GAAGTCATTC TTCTGGCAAA GATGAAACAT
    CCCAACATTG TAACCTTCTT CAATTCATTT CAAGAGAACA ACAGACTGTT TATTGTGATG
    GAGTATTGTG ATGGCGGGGA TCTCATGAAA AGGATCAGAA GACAACGGGG AGTGTTATTT
    AGTGAAGATC AGATTCTGAG TTGGTTTGTA CAGATTTCTC TAGGACTGAA ACATATTCAC
    GACAGGAAGG TTTTACACAG GGACATAAAA ACACAGAACA TTTTTCTTAG CAAGAACGGA
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGACACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG TTTCAGGATA TTGAGAGAGA CTTAAAACAA
    ATGAAACCTC AGAACATAAA GGAAAGTAAA AGTTTGGAAC AAAAACATAA AGCTCAGAGA
    GGGCTAAAAT TTGAAATTAA TTTAAATGAA TGTATTTCTG ATGAAAACAC CCTCCAAGAG
    GAAGAGGTAG TGGATATACT GAATGAAACT TTGATCTCTG AGGATGGCAT GAAGTTCATG
    GAATATAAAT GTATGAAGGA GCAGGAAGAT TATACAGACC AAGCATTTGA AACACTCTGT
    TGCCCAGAAG CAGAGTTGTG CATTCCGGAT GCCACTGCTG CACAGCACAG GAGACAGTGG
    GCCCCAGGAG CTCCTCAGAC TCTGCTGCAA ATCATGGCCT CGGCCGATGT CACCTCCACC
    TGCTCCACCT GCTCCACCAT GCCTGAGGAT GGCCGAGTGA TTGAGATTGA AGGCGGTCCA
    GAAACCAGGA GACGGTGGCA GCGAGAAGCA CCGGGAACTT TACTGAGTGT TTTGGCAGCA
    GCACAGCTAA CCAGGAGCTC GCTTTCTGCC AACGATGGAG AATGTGTGGG ACCACTGAAA
    GAAGATAAGG GGAAGGTGGA AGTGGCCTCT GACATTGAAA TAGATGCAGA ACAACCAGAT
    CCAAGATCTG ATGACGATGA TACAAATTTT GAAGAATCTG AAGATGAATT GAGAAATGAA
    GTAGTAGAAT CTCTGGAAAA ACTGGCTACT TTCAATGAGG CAGAAAAAAG AGAAGAGGTT
    TCTAGTTCTT CTAAAGATGC TGGAAACTTA GAAGAAACAG AGGGGATAGG CATGCAGAAA
    TATTCAGAAT TAAATGAAGG TTTGGAGAGT ATTTTTATTC CATCTGATGA CAAAATTTGT
    ATTGGTGATG AAGATCAAGG AGCATCAACC AACAGTCAAA ATATTCAACT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006065814.2
    CDS126..2639
    Translation

    Target ORF information:

    RefSeq Version XM_006065752.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis serine/threonine-protein kinase Nek5 (LOC102389575), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006065752.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATCAAT ATGACATGAT TAAAGCCATT GGGGAAGGTG CCTTTGGGAA AGCGTACTTG 
    GCCCAAAGGA AATCGGATAG CGAGCACTGT GTTATAAAAG AGATCAATTT TGAAAAGATG
    CCCATCCAAG AAAAAGAAGC TTCCAAGAAA GAAGTCATTC TTCTGGCAAA GATGAAACAT
    CCCAACATTG TAACCTTCTT CAATTCATTT CAAGAGAACA ACAGACTGTT TATTGTGATG
    GAGTATTGTG ATGGCGGGGA TCTCATGAAA AGGATCAGAA GACAACGGGG AGTGTTATTT
    AGTGAAGATC AGATTCTGAG TTGGTTTGTA CAGATTTCTC TAGGACTGAA ACATATTCAC
    GACAGGAAGG TTTTACACAG GGACATAAAA ACACAGAACA TTTTTCTTAG CAAGAACGGA
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGACACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG TTTCAGGATA TTGAGAGAGA CTTAAAACAA
    ATGAAACCTC AGAACATAAA GGAAAGTAAA AGTTTGGAAC AAAAACATAA AGCTCAGAGA
    GGGCTAAAAT TTGAAATTAA TTTAAATGAA TGTATTTCTG ATGAAAACAC CCTCCAAGAG
    GAAGAGGTAG TGGATATACT GAATGAAACT TTGATCTCTG AGGATGGCAT GAAGTTCATG
    GAATATAAAT GTATGAAGGA GCAGGAAGAT TATACAGACC AAGCATTTGA AACACTCTGT
    TGCCCAGAAG CAGAGTTGTG CATTCCGGAT GCCACTGCTG CACAGCACAG GAGACAGTGG
    GCCCCAGGAG CTCCTCAGAC TCTGCTGCAA ATCATGGCCT CGGCCGATGT CACCTCCACC
    TGCTCCACCT GCTCCACCAT GCCTGAGGAT GGCCGAGTGA TTGAGATTGA AGGCGGTCCA
    GAAACCAGGA GACGGTGGCA GCGAGAAGCA CCGGGAACTT TACTGAGTGT TTTGGCAGCA
    GCACAGCTAA CCAGGAGCTC GCTTTCTGCC AACGATGGAG AATGTGTGGG ACCACTGAAA
    GAAGATAAGG GGAAGGTGGA AGTGGCCTCT GACATTGAAA TAGATGCAGA ACAACCAGAT
    CCAAGATCTG ATGACGATGA TACAAATTTT GAAGAATCTG AAGATGAATT GAGAAATGAA
    GTAGTAGAAT CTCTGGAAAA ACTGGCTACT TTCAATGAGG CAGAAAAAAG AGAAGAGGTT
    TCTAGTTCTT CTAAAGATGC TGGAAACTTA GAAGAAACAG AGGGGATAGG CATGCAGAAA
    TATTCAGAAT TAAATGAAGG TTTGGAGAGT ATTTTTATTC CATCTGATGA CAAAATTTGT
    ATTGGTGATG AAGATCAAGG AGCATCAACC AACAGTCAAA ATATTCAACT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu47605
    Clone ID Related Accession (Same CDS sequence) XM_006065753.2
    Accession Version XM_006065753.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product serine/threonine-protein kinase Nek5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006065753.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATCAAT ATGACATGAT TAAAGCCATT GGGGAAGGTG CCTTTGGGAA AGCGTACTTG 
    GCCCAAAGGA AATCGGATAG CGAGCACTGT GTTATAAAAG AGATCAATTT TGAAAAGATG
    CCCATCCAAG AAAAAGAAGC TTCCAAGAAA GAAGTCATTC TTCTGGCAAA GATGAAACAT
    CCCAACATTG TAACCTTCTT CAATTCATTT CAAGAGAACA ACAGACTGTT TATTGTGATG
    GAGTATTGTG ATGGCGGGGA TCTCATGAAA AGGATCAGAA GACAACGGGG AGTGTTATTT
    AGTGAAGATC AGATTCTGAG TTGGTTTGTA CAGATTTCTC TAGGACTGAA ACATATTCAC
    GACAGGAAGG TTTTACACAG GGACATAAAA ACACAGAACA TTTTTCTTAG CAAGAACGGA
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGACACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG GATATTGAGA GAGACTTAAA ACAAATGAAA
    CCTCAGAACA TAAAGGAAAG TAAAAGTTTG GAACAAAAAC ATAAAGCTCA GAGAGGGCTA
    AAATTTGAAA TTAATTTAAA TGAATGTATT TCTGATGAAA ACACCCTCCA AGAGGAAGAG
    GTAGTGGATA TACTGAATGA AACTTTGATC TCTGAGGATG GCATGAAGTT CATGGAATAT
    AAATGTATGA AGGAGCAGGA AGATTATACA GACCAAGCAT TTGAAACACT CTGTTGCCCA
    GAAGCAGAGT TGTGCATTCC GGATGCCACT GCTGCACAGC ACAGGAGACA GTGGGCCCCA
    GGAGCTCCTC AGACTCTGCT GCAAATCATG GCCTCGGCCG ATGTCACCTC CACCTGCTCC
    ACCTGCTCCA CCATGCCTGA GGATGGCCGA GTGATTGAGA TTGAAGGCGG TCCAGAAACC
    AGGAGACGGT GGCAGCGAGA AGCACCGGGA ACTTTACTGA GTGTTTTGGC AGCAGCACAG
    CTAACCAGGA GCTCGCTTTC TGCCAACGAT GGAGAATGTG TGGGACCACT GAAAGAAGAT
    AAGGGGAAGG TGGAAGTGGC CTCTGACATT GAAATAGATG CAGAACAACC AGATCCAAGA
    TCTGATGACG ATGATACAAA TTTTGAAGAA TCTGAAGATG AATTGAGAAA TGAAGTAGTA
    GAATCTCTGG AAAAACTGGC TACTTTCAAT GAGGCAGAAA AAAGAGAAGA GGTTTCTAGT
    TCTTCTAAAG ATGCTGGAAA CTTAGAAGAA ACAGAGGGGA TAGGCATGCA GAAATATTCA
    GAATTAAATG AAGGTTTGGA GAGTATTTTT ATTCCATCTG ATGACAAAAT TTGTATTGGT
    GATGAAGATC AAGGAGCATC AACCAACAGT CAAAATATTC AACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006065815.2
    CDS126..2633
    Translation

    Target ORF information:

    RefSeq Version XM_006065753.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis serine/threonine-protein kinase Nek5 (LOC102389575), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006065753.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATCAAT ATGACATGAT TAAAGCCATT GGGGAAGGTG CCTTTGGGAA AGCGTACTTG 
    GCCCAAAGGA AATCGGATAG CGAGCACTGT GTTATAAAAG AGATCAATTT TGAAAAGATG
    CCCATCCAAG AAAAAGAAGC TTCCAAGAAA GAAGTCATTC TTCTGGCAAA GATGAAACAT
    CCCAACATTG TAACCTTCTT CAATTCATTT CAAGAGAACA ACAGACTGTT TATTGTGATG
    GAGTATTGTG ATGGCGGGGA TCTCATGAAA AGGATCAGAA GACAACGGGG AGTGTTATTT
    AGTGAAGATC AGATTCTGAG TTGGTTTGTA CAGATTTCTC TAGGACTGAA ACATATTCAC
    GACAGGAAGG TTTTACACAG GGACATAAAA ACACAGAACA TTTTTCTTAG CAAGAACGGA
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGACACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG GATATTGAGA GAGACTTAAA ACAAATGAAA
    CCTCAGAACA TAAAGGAAAG TAAAAGTTTG GAACAAAAAC ATAAAGCTCA GAGAGGGCTA
    AAATTTGAAA TTAATTTAAA TGAATGTATT TCTGATGAAA ACACCCTCCA AGAGGAAGAG
    GTAGTGGATA TACTGAATGA AACTTTGATC TCTGAGGATG GCATGAAGTT CATGGAATAT
    AAATGTATGA AGGAGCAGGA AGATTATACA GACCAAGCAT TTGAAACACT CTGTTGCCCA
    GAAGCAGAGT TGTGCATTCC GGATGCCACT GCTGCACAGC ACAGGAGACA GTGGGCCCCA
    GGAGCTCCTC AGACTCTGCT GCAAATCATG GCCTCGGCCG ATGTCACCTC CACCTGCTCC
    ACCTGCTCCA CCATGCCTGA GGATGGCCGA GTGATTGAGA TTGAAGGCGG TCCAGAAACC
    AGGAGACGGT GGCAGCGAGA AGCACCGGGA ACTTTACTGA GTGTTTTGGC AGCAGCACAG
    CTAACCAGGA GCTCGCTTTC TGCCAACGAT GGAGAATGTG TGGGACCACT GAAAGAAGAT
    AAGGGGAAGG TGGAAGTGGC CTCTGACATT GAAATAGATG CAGAACAACC AGATCCAAGA
    TCTGATGACG ATGATACAAA TTTTGAAGAA TCTGAAGATG AATTGAGAAA TGAAGTAGTA
    GAATCTCTGG AAAAACTGGC TACTTTCAAT GAGGCAGAAA AAAGAGAAGA GGTTTCTAGT
    TCTTCTAAAG ATGCTGGAAA CTTAGAAGAA ACAGAGGGGA TAGGCATGCA GAAATATTCA
    GAATTAAATG AAGGTTTGGA GAGTATTTTT ATTCCATCTG ATGACAAAAT TTGTATTGGT
    GATGAAGATC AAGGAGCATC AACCAACAGT CAAAATATTC AACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu47606
    Clone ID Related Accession (Same CDS sequence) XM_025262949.1
    Accession Version XM_025262949.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2445bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product serine/threonine-protein kinase Nek5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATCAAT ATGACATGAT TAAAGCCATT GGGGAAGGTG CCTTTGGGAA AGCGTACTTG 
    GCCCAAAGGA AATCGGATAG CGAGCACTGT GTTATAAAAG AGATCAATTT TGAAAAGATG
    CCCATCCAAG AAAAAGAAGC TTCCAAGAAA GAAGTCATTC TTCTGGCAAA GATGAAACAT
    CCCAACATTG TAACCTTCTT CAATTCATTT CAAGAGAACA ACAGACTGTT TATTGTGATG
    GAGTATTGTG ATGGCGGGGA TCTCATGAAA AGGATCAGAA GACAACGGGG AGTGTTATTT
    AGTGAAGATC AGATTCTGAG TTGGTTTGTA CAGATTTCTC TAGGACTGAA ACATATTCAC
    GACAGGAAGG TTTTACACAG GGACATAAAA ACACAGAACA TTTTTCTTAG CAAGAACGGA
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGACACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG TTTCAGGATA TTGAGAGAGA CTTAAAACAA
    ATGAAACCTC AGAACATAAA GGAAAGTAAA AGTTTGGAAC AAAAACATAA AGCTCAGGTA
    GTGGATATAC TGAATGAAAC TTTGATCTCT GAGGATGGCA TGAAGTTCAT GGAATATAAA
    TGTATGAAGG AGCAGGAAGA TTATACAGAC CAAGCATTTG AAACACTCTG TTGCCCAGAA
    GCAGAGTTGT GCATTCCGGA TGCCACTGCT GCACAGCACA GGAGACAGTG GGCCCCAGGA
    GCTCCTCAGA CTCTGCTGCA AATCATGGCC TCGGCCGATG TCACCTCCAC CTGCTCCACC
    TGCTCCACCA TGCCTGAGGA TGGCCGAGTG ATTGAGATTG AAGGCGGTCC AGAAACCAGG
    AGACGGTGGC AGCGAGAAGC ACCGGGAACT TTACTGAGTG TTTTGGCAGC AGCACAGCTA
    ACCAGGAGCT CGCTTTCTGC CAACGATGGA GAATGTGTGG GACCACTGAA AGAAGATAAG
    GGGAAGGTGG AAGTGGCCTC TGACATTGAA ATAGATGCAG AACAACCAGA TCCAAGATCT
    GATGACGATG ATACAAATTT TGAAGAATCT GAAGATGAAT TGAGAAATGA AGTAGTAGAA
    TCTCTGGAAA AACTGGCTAC TTTCAATGAG GCAGAAAAAA GAGAAGAGGT TTCTAGTTCT
    TCTAAAGATG CTGGAAACTT AGAAGAAACA GAGGGGATAG GCATGCAGAA ATATTCAGAA
    TTAAATGAAG GTTTGGAGAG TATTTTTATT CCATCTGATG ACAAAATTTG TATTGGTGAT
    GAAGATCAAG GAGCATCAAC CAACAGTCAA AATATTCAAC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025118734.1
    CDS126..2570
    Translation

    Target ORF information:

    RefSeq Version XM_025262949.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis serine/threonine-protein kinase Nek5 (LOC102389575), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025262949.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATCAAT ATGACATGAT TAAAGCCATT GGGGAAGGTG CCTTTGGGAA AGCGTACTTG 
    GCCCAAAGGA AATCGGATAG CGAGCACTGT GTTATAAAAG AGATCAATTT TGAAAAGATG
    CCCATCCAAG AAAAAGAAGC TTCCAAGAAA GAAGTCATTC TTCTGGCAAA GATGAAACAT
    CCCAACATTG TAACCTTCTT CAATTCATTT CAAGAGAACA ACAGACTGTT TATTGTGATG
    GAGTATTGTG ATGGCGGGGA TCTCATGAAA AGGATCAGAA GACAACGGGG AGTGTTATTT
    AGTGAAGATC AGATTCTGAG TTGGTTTGTA CAGATTTCTC TAGGACTGAA ACATATTCAC
    GACAGGAAGG TTTTACACAG GGACATAAAA ACACAGAACA TTTTTCTTAG CAAGAACGGA
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGACACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG TTTCAGGATA TTGAGAGAGA CTTAAAACAA
    ATGAAACCTC AGAACATAAA GGAAAGTAAA AGTTTGGAAC AAAAACATAA AGCTCAGGTA
    GTGGATATAC TGAATGAAAC TTTGATCTCT GAGGATGGCA TGAAGTTCAT GGAATATAAA
    TGTATGAAGG AGCAGGAAGA TTATACAGAC CAAGCATTTG AAACACTCTG TTGCCCAGAA
    GCAGAGTTGT GCATTCCGGA TGCCACTGCT GCACAGCACA GGAGACAGTG GGCCCCAGGA
    GCTCCTCAGA CTCTGCTGCA AATCATGGCC TCGGCCGATG TCACCTCCAC CTGCTCCACC
    TGCTCCACCA TGCCTGAGGA TGGCCGAGTG ATTGAGATTG AAGGCGGTCC AGAAACCAGG
    AGACGGTGGC AGCGAGAAGC ACCGGGAACT TTACTGAGTG TTTTGGCAGC AGCACAGCTA
    ACCAGGAGCT CGCTTTCTGC CAACGATGGA GAATGTGTGG GACCACTGAA AGAAGATAAG
    GGGAAGGTGG AAGTGGCCTC TGACATTGAA ATAGATGCAG AACAACCAGA TCCAAGATCT
    GATGACGATG ATACAAATTT TGAAGAATCT GAAGATGAAT TGAGAAATGA AGTAGTAGAA
    TCTCTGGAAA AACTGGCTAC TTTCAATGAG GCAGAAAAAA GAGAAGAGGT TTCTAGTTCT
    TCTAAAGATG CTGGAAACTT AGAAGAAACA GAGGGGATAG GCATGCAGAA ATATTCAGAA
    TTAAATGAAG GTTTGGAGAG TATTTTTATT CCATCTGATG ACAAAATTTG TATTGGTGAT
    GAAGATCAAG GAGCATCAAC CAACAGTCAA AATATTCAAC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu47607
    Clone ID Related Accession (Same CDS sequence) XM_025262950.1
    Accession Version XM_025262950.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2379bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product serine/threonine-protein kinase Nek5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGATCAAT ATGACATGAT TAAAGCCATT GGGGAAGGTG CCTTTGGGAA AGCGTACTTG 
    GCCCAAAGGA AATCGGATAG CGAGCACTGT GTTATAAAAG AGATCAATTT TGAAAAGATG
    CCCATCCAAG AAAAAGAAGC TTCCAAGAAA GAAGTCATTC TTCTGGCAAA GATGAAACAT
    CCCAACATTG TAACCTTCTT CAATTCATTT CAAGAGAACA ACAGACTGTT TATTGTGATG
    GAGTATTGTG ATGGCGGGGA TCTCATGAAA AGGATCAGAA GACAACGGGG AGTGTTATTT
    AGTGAAGATC AGATTCTGAG TTGGTTTGTA CAGATTTCTC TAGGACTGAA ACATATTCAC
    GACAGGAAGG TTTTACACAG GGACATAAAA ACACAGAACA TTTTTCTTAG CAAGAACGGA
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGACACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG TTTCAGGATA TTGAGAGAGA CTTAAAACAA
    ATGAAACCTC AGAACATAAA GGAAAGTAAA AGTTTGGAAC AAAAACATAA AGCTCAGAGA
    GGGCTAAAAT TTGAAATTAA TTTAAATGAA TGTATTTCTG ATGAAAACAC CCTCCAAGAG
    GAAGAGGTAG TGGATATACT GAATGAAACT TTGATCTCTG AGGATGGCAT GAAGTTCATG
    GAATATAAAT GTATGAAGGA GCAGGAAGAT TATACAGACC AAGCATTTGA AACACTCTGT
    TGCCCAGAAG CAGATGGCCG AGTGATTGAG ATTGAAGGCG GTCCAGAAAC CAGGAGACGG
    TGGCAGCGAG AAGCACCGGG AACTTTACTG AGTGTTTTGG CAGCAGCACA GCTAACCAGG
    AGCTCGCTTT CTGCCAACGA TGGAGAATGT GTGGGACCAC TGAAAGAAGA TAAGGGGAAG
    GTGGAAGTGG CCTCTGACAT TGAAATAGAT GCAGAACAAC CAGATCCAAG ATCTGATGAC
    GATGATACAA ATTTTGAAGA ATCTGAAGAT GAATTGAGAA ATGAAGTAGT AGAATCTCTG
    GAAAAACTGG CTACTTTCAA TGAGGCAGAA AAAAGAGAAG AGGTTTCTAG TTCTTCTAAA
    GATGCTGGAA ACTTAGAAGA AACAGAGGGG ATAGGCATGC AGAAATATTC AGAATTAAAT
    GAAGGTTTGG AGAGTATTTT TATTCCATCT GATGACAAAA TTTGTATTGG TGATGAAGAT
    CAAGGAGCAT CAACCAACAG TCAAAATATT CAACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025118735.1
    CDS126..2504
    Translation

    Target ORF information:

    RefSeq Version XM_025262950.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis serine/threonine-protein kinase Nek5 (LOC102389575), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025262950.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGATCAAT ATGACATGAT TAAAGCCATT GGGGAAGGTG CCTTTGGGAA AGCGTACTTG 
    GCCCAAAGGA AATCGGATAG CGAGCACTGT GTTATAAAAG AGATCAATTT TGAAAAGATG
    CCCATCCAAG AAAAAGAAGC TTCCAAGAAA GAAGTCATTC TTCTGGCAAA GATGAAACAT
    CCCAACATTG TAACCTTCTT CAATTCATTT CAAGAGAACA ACAGACTGTT TATTGTGATG
    GAGTATTGTG ATGGCGGGGA TCTCATGAAA AGGATCAGAA GACAACGGGG AGTGTTATTT
    AGTGAAGATC AGATTCTGAG TTGGTTTGTA CAGATTTCTC TAGGACTGAA ACATATTCAC
    GACAGGAAGG TTTTACACAG GGACATAAAA ACACAGAACA TTTTTCTTAG CAAGAACGGA
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGACACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG TTTCAGGATA TTGAGAGAGA CTTAAAACAA
    ATGAAACCTC AGAACATAAA GGAAAGTAAA AGTTTGGAAC AAAAACATAA AGCTCAGAGA
    GGGCTAAAAT TTGAAATTAA TTTAAATGAA TGTATTTCTG ATGAAAACAC CCTCCAAGAG
    GAAGAGGTAG TGGATATACT GAATGAAACT TTGATCTCTG AGGATGGCAT GAAGTTCATG
    GAATATAAAT GTATGAAGGA GCAGGAAGAT TATACAGACC AAGCATTTGA AACACTCTGT
    TGCCCAGAAG CAGATGGCCG AGTGATTGAG ATTGAAGGCG GTCCAGAAAC CAGGAGACGG
    TGGCAGCGAG AAGCACCGGG AACTTTACTG AGTGTTTTGG CAGCAGCACA GCTAACCAGG
    AGCTCGCTTT CTGCCAACGA TGGAGAATGT GTGGGACCAC TGAAAGAAGA TAAGGGGAAG
    GTGGAAGTGG CCTCTGACAT TGAAATAGAT GCAGAACAAC CAGATCCAAG ATCTGATGAC
    GATGATACAA ATTTTGAAGA ATCTGAAGAT GAATTGAGAA ATGAAGTAGT AGAATCTCTG
    GAAAAACTGG CTACTTTCAA TGAGGCAGAA AAAAGAGAAG AGGTTTCTAG TTCTTCTAAA
    GATGCTGGAA ACTTAGAAGA AACAGAGGGG ATAGGCATGC AGAAATATTC AGAATTAAAT
    GAAGGTTTGG AGAGTATTTT TATTCCATCT GATGACAAAA TTTGTATTGG TGATGAAGAT
    CAAGGAGCAT CAACCAACAG TCAAAATATT CAACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu47608
    Clone ID Related Accession (Same CDS sequence) XM_006065754.2
    Accession Version XM_006065754.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2358bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product serine/threonine-protein kinase Nek5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006065754.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGATCAAT ATGACATGAT TAAAGCCATT GGGGAAGGTG CCTTTGGGAA AGCGTACTTG 
    GCCCAAAGGA AATCGGATAG CGAGCACTGT GTTATAAAAG AGATCAATTT TGAAAAGATG
    CCCATCCAAG AAAAAGAAGC TTCCAAGAAA GAAGTCATTC TTCTGGCAAA GATGAAACAT
    CCCAACATTG TAACCTTCTT CAATTCATTT CAAGAGAACA ACAGACTGTT TATTGTGATG
    GAGTATTGTG ATGGCGGGGA TCTCATGAAA AGGATCAGAA GACAACGGGG AGTGTTATTT
    AGTGAAGATC AGATTCTGAG TTGGTTTGTA CAGATTTCTC TAGGACTGAA ACATATTCAC
    GACAGGAAGG TTTTACACAG GGACATAAAA ACACAGAACA TTTTTCTTAG CAAGAACGGA
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGACACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG GTAGTGGATA TACTGAATGA AACTTTGATC
    TCTGAGGATG GCATGAAGTT CATGGAATAT AAATGTATGA AGGAGCAGGA AGATTATACA
    GACCAAGCAT TTGAAACACT CTGTTGCCCA GAAGCAGAGT TGTGCATTCC GGATGCCACT
    GCTGCACAGC ACAGGAGACA GTGGGCCCCA GGAGCTCCTC AGACTCTGCT GCAAATCATG
    GCCTCGGCCG ATGTCACCTC CACCTGCTCC ACCTGCTCCA CCATGCCTGA GGATGGCCGA
    GTGATTGAGA TTGAAGGCGG TCCAGAAACC AGGAGACGGT GGCAGCGAGA AGCACCGGGA
    ACTTTACTGA GTGTTTTGGC AGCAGCACAG CTAACCAGGA GCTCGCTTTC TGCCAACGAT
    GGAGAATGTG TGGGACCACT GAAAGAAGAT AAGGGGAAGG TGGAAGTGGC CTCTGACATT
    GAAATAGATG CAGAACAACC AGATCCAAGA TCTGATGACG ATGATACAAA TTTTGAAGAA
    TCTGAAGATG AATTGAGAAA TGAAGTAGTA GAATCTCTGG AAAAACTGGC TACTTTCAAT
    GAGGCAGAAA AAAGAGAAGA GGTTTCTAGT TCTTCTAAAG ATGCTGGAAA CTTAGAAGAA
    ACAGAGGGGA TAGGCATGCA GAAATATTCA GAATTAAATG AAGGTTTGGA GAGTATTTTT
    ATTCCATCTG ATGACAAAAT TTGTATTGGT GATGAAGATC AAGGAGCATC AACCAACAGT
    CAAAATATTC AACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006065816.2
    CDS126..2483
    Translation

    Target ORF information:

    RefSeq Version XM_006065754.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis serine/threonine-protein kinase Nek5 (LOC102389575), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006065754.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGATCAAT ATGACATGAT TAAAGCCATT GGGGAAGGTG CCTTTGGGAA AGCGTACTTG 
    GCCCAAAGGA AATCGGATAG CGAGCACTGT GTTATAAAAG AGATCAATTT TGAAAAGATG
    CCCATCCAAG AAAAAGAAGC TTCCAAGAAA GAAGTCATTC TTCTGGCAAA GATGAAACAT
    CCCAACATTG TAACCTTCTT CAATTCATTT CAAGAGAACA ACAGACTGTT TATTGTGATG
    GAGTATTGTG ATGGCGGGGA TCTCATGAAA AGGATCAGAA GACAACGGGG AGTGTTATTT
    AGTGAAGATC AGATTCTGAG TTGGTTTGTA CAGATTTCTC TAGGACTGAA ACATATTCAC
    GACAGGAAGG TTTTACACAG GGACATAAAA ACACAGAACA TTTTTCTTAG CAAGAACGGA
    ATGGTTGCAA AGCTTGGGGA CTTTGGTATT GCAAGGCTCT TGAACAATAC CATGGAACTT
    GCTAGAACTT GCGTTGGAAC ACCATACTAC CTGTCCCCAG AGATCTGTCA GAATAAACCC
    TACAACAATA AAACGGATAT TTGGTCTCTT GGCTGTGTCT TATATGAGCT CTGCACACTC
    AGACATCCTT TTGAGGGTAA CAACTTACAG CAGCTGGTTC TGAAGATTTG TCAAGCTCAT
    GTTCCTCCGA TATCGCCAAG GTTTTCCCGT GACCTACAGT TCTTAATATC CCAGCTCTTT
    GAAGTATCTC CCCGCGATCG GCCATCCATC AATTCCATTT TGAAAAGGCC CTTTTTAGAG
    AATCTTATTG CCAAATACTT GACTCCCGAG GTCATTCACG AAGAATTCAG TCACCCACTC
    TTACATAAAG CAAGACCATC TGCTTCTCGA CCTGCTGGGA AGGTGGCTCC TGATTCTAAA
    ATACAGAAAG TGAGATTCCA GGGAAAGTGT CTACCACAAT CAAGGATAAC AGTGCCAAAT
    AAGAGGAAGG ACGTATTACG TAGAAATGAA TGGAGATCAC CAGCTGGAGC CCAGACACCC
    ATATCTGTAA AAATGGTAGA AAGGCCCAAA CTTGCTGCAG TGTGTGGACA TTATGATCAT
    TTTTATGCTC AACTTGACTT GTTGAGGAAG AGAGCACATG GACCAAATTA CCACCGTGTT
    CCTCAAAAAG ATAGCAGAGA TGAGGAGAGT TATGATCAGG AAGAAAGCCA CAGTCCACCT
    CCAGGTCAAT GGCTTGCTGA ATACCTTCAG AGGAAATGTG AAGCCCAACA ATATAAGCTG
    AAAGTGGAAA AGCAATTGGG TCTTCGTCCA TCTTCTGCTG AGCCAAATGA CAACCAGAGA
    CAAAAGCTAA GAAGCGATGG GGAAGGACCT AGATTCTGGG GGCTGCAGTT CAGGAGAAAT
    GAAATGAAGG AACAGGAATA TTGGAAGCAG TTAGAGGAAA TACGCCAACA GTATCACCAT
    GACATGAAGG AAATTAGAAA GAAGATGGGG TGTGAACTGG AGGAGGACTC AAAAATGAGC
    TGGAAAACCT ACTTGGTGAA GAAGAGTGAC CTGCCCATCC CCCATGAAGC ACCTGAGGAA
    GAAGCACCTG AGGAAGAAGC ACCTGTGCAG GTAGTGGATA TACTGAATGA AACTTTGATC
    TCTGAGGATG GCATGAAGTT CATGGAATAT AAATGTATGA AGGAGCAGGA AGATTATACA
    GACCAAGCAT TTGAAACACT CTGTTGCCCA GAAGCAGAGT TGTGCATTCC GGATGCCACT
    GCTGCACAGC ACAGGAGACA GTGGGCCCCA GGAGCTCCTC AGACTCTGCT GCAAATCATG
    GCCTCGGCCG ATGTCACCTC CACCTGCTCC ACCTGCTCCA CCATGCCTGA GGATGGCCGA
    GTGATTGAGA TTGAAGGCGG TCCAGAAACC AGGAGACGGT GGCAGCGAGA AGCACCGGGA
    ACTTTACTGA GTGTTTTGGC AGCAGCACAG CTAACCAGGA GCTCGCTTTC TGCCAACGAT
    GGAGAATGTG TGGGACCACT GAAAGAAGAT AAGGGGAAGG TGGAAGTGGC CTCTGACATT
    GAAATAGATG CAGAACAACC AGATCCAAGA TCTGATGACG ATGATACAAA TTTTGAAGAA
    TCTGAAGATG AATTGAGAAA TGAAGTAGTA GAATCTCTGG AAAAACTGGC TACTTTCAAT
    GAGGCAGAAA AAAGAGAAGA GGTTTCTAGT TCTTCTAAAG ATGCTGGAAA CTTAGAAGAA
    ACAGAGGGGA TAGGCATGCA GAAATATTCA GAATTAAATG AAGGTTTGGA GAGTATTTTT
    ATTCCATCTG ATGACAAAAT TTGTATTGGT GATGAAGATC AAGGAGCATC AACCAACAGT
    CAAAATATTC AACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.