LOC103866418 cDNA ORF clone, Brassica rapa(field mustard)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103866418
Entrez Gene ID	103866418
Full Name	protein phosphatase 2C 32
Gene Type	protein-coding
Organism	Brassica rapa(field mustard)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009144334.2	XP_009142582.1	protein phosphatase 2C 32
XM_009144333.2	XP_009142581.1	protein phosphatase 2C 32

Pathways from BioSystems
Gene Ontology

The following LOC103866418 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103866418 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr149949	XM_009144333.2 Latest version!	Brassica rapa protein phosphatase 2C 32 (LOC103866418), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBr149949	XM_009144334.2 Latest version!	Brassica rapa protein phosphatase 2C 32 (LOC103866418), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103866418 ( XM_009144334.2 ) cDNA ORF clone, Brassica rapa(field mustard) -> XP_009142582.1 Brassica rapa protein phosphatase 2C 32 (LOC103866418), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBr149949
Clone ID Related Accession (Same CDS sequence)	XM_009144334.2 , XM_009144333.2
Accession Version	XM_009144334.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-12
Organism	Brassica rapa(field mustard)
Product	protein phosphatase 2C 32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024799.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 13, 2016 this sequence version replaced XM_009144334.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brassica rapa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACG GAACTTCCCG CGTTGTCGGC TGTTTCGTGC CGTCTAACGA CAAAAACGGC 
GTTGATTCGG AGTTTCTTGA GCCTCTAGAC GAAGGTTTAG GGCATTCCTT TTGCTACGTT 
AGGCCGAGCA TCTTCGAGTC TCCGGACATC ACTCCTTCTA ACTCGGAGAG GTTCACTATT 
GACTCAAGCA CGCTTGACTC TGAGACTCTC AGTGGATCAT TCCGAAACGA GGTCGTGCTT 
GATGATCCTT CGTTCTTGAA CAGACACAAC AGTAAAGGTT TGGGGGAGAC GACATTTAAG 
GCGATCTCAG GTGCTTCGGT TAGTGCCAAT GTGTCTACAG CTAGGACTGG GAATCAAACG 
GCGTTGTGTT CAAGTGACGT GTTGGAGCCT GCTGCTACAT TTGAGAGCAC GTCTTCTTTT 
GCTTCAATTC CTTTGCAACC ACTCCCTCGT GGTGGTTCCG GACCGTTGAA CGGGTTCATG 
TCAGGGCCTT TAGAGAGAGG TTTTGCGTCT GGTCCTTTGG ATAGAAACAA TGGTTTCATG 
TCAGGGCCTA TAGAGAAAGG AGTCATGTCT GGTCCTCTTG ATGTATCTGA TAAATCTAAT 
TTCTCGGCTC CGCTTTCTTT TAGACGTAGA AAGCCTCGGT TTCAGCGGTT TATGAGGAGT 
GTAAGCGGAC CAATGAGAAG TACATTAGCA AGAACATTCT CCAGAAGGTC TGGAGGTGGT 
TTAAGCTGGA TGCAACGCTT CTTCTTGCAT CCAGAGAGTA GAGTGTCTTG GCCTGGGGGG 
AAAGAAGGTA AATTACATGG TGAAGACCAC CCGGAGAGTT GTCTAGAGAG CAACCGTCAC 
TTGCAGTGGG CTCATGGGAG AGCTGGAGAA GACAGGGTTC ATGTTGTGCT TTCGGAGGAG 
CAAGGATGGC TCTTCATAGG GATATATGAT GGGTTTAGTG GACCGGATGC TCCGGACTTC 
GTGATGAGTC ATCTGTACAA AGCTATAGAC AAGGAACTGG AAGGTCTCCT CTGGGTTTAT 
GACGAGCCAT CTGAAGATAA TCCATTGCAG CCAGACCAGG AATCTCATTC TACTACACAG 
GGTAACTTGT TAGTTCCTGA AACTATCAGT GAGCAGCATT CAAAGTCAGC ACAAGAAGGA 
AGTGAAGAGG TTATGACTGA TAATAACAGT AGCCTTGAAA ATGCCGATGG TCCACCTGGA 
AATTTAGCAG GTCCTGGCAA GAAAAGCACG AGGCTTTACG AGCTGCTTCA GTTGGAACGG 
TGGGATGGAG AAGAAGTGGG ACTTAAACAT TCTGTAAGTG ATATGACCAA TCAGGTTGAA 
GATGCATCAA CTTCAGGTGG AAATGATCCG TGTACCACAG AGAACACCGT TCTTGATGAG 
ATTCCAAACT CAGGGCAAAT CCATGGGACT AAAAAATCAC AGATAAGCTC AAAGATAAGA 
AGAATGTATC AAAAACAAAA GTCTTTACGG AAAAAGCTGT TCCCATGGAG TTATGATTGG 
CACAGGGAAG AAGAAGGGAC GACTTGCGTT GAAGAGAAGA TCGTAGAGTC ATCAGGACCG 
ATTAGGAGAC GCTGGTCAGG AACCGTAGAC CATGATGATG TGCTAAGAGC AATGGCAAGA 
GCACTAGAAA GCACTGAGGA GGCTTACATG GAAATGGTGG AGAAGTCCCT TGACATAAAC 
CCGGAGCTTG CGTTAATGGG TTCATGTGTT CTCGTAATGC TAATGAAGGA TCAAGATGTT 
TACGTGATGA ATGTTGGTGA TAGTCGAGCT ATCTTGGCTC AGGAAAGGCT CCATGATCGT 
CATTCTAATC CAGGGTTTGG AGTTGAGGAA GGTATGGGGC ATAAGAGTAG GTCGAGAGAG 
TCGCTAGTGC GTATGGAACT AGATAGAATA TCAGAAGAAT CTCCAATACA CAATCTAACC 
ACTCAGATTA GTGTCTCAAA CAAAAACAGA GATGTAACTT CTTACCGGTT AAAGATGAGA 
GCTGTTCAAC TCTCAAGTGA CCATAGCACA AGTGTAGAAG AGGAAGTTTC AAGAATTAGA 
TCAGAACATC CAGAAGATGA GCAGTCCATA CTCAAAGACA GAGTCAAAGG CCAGCTCAAA 
GTCACTCGAG CTTTTGGTGC TGGTTTTCTC AAAAAGCCGA GTTTCAATGA AGCTTTGCTT 
GAGATGTTCA GAGTAGACTA CATTGGAACA GATCCGTATA TCAGCTGCGA ACCGTGTACT 
GTCCATCACA GGCTCACTTC AAGCGATCGG TTCTTGGTTT TATGCTCTGA TGGATTGTAT 
GAATACTTCA GCAACGAAGA GATTGTTGCT CACGTGACTT GGTTCATTGA AAACGTTCCT 
GATGGTGATC CTGCTCAATA CCTCATTGCA GAGCTTCTCT CTCGTGCAGC CACCAAGAAC 
GGGATGGAGT TTCATGATCT GTTGGATATA CCTCAAGGAG ATAGAAGGAA GTACCATGAT 
GATGTATCTG TGATGGTTGT GTCTCTTGAA GGAAGGATTT GGCGATCGTC TGGACAATAT 
TATCCTGAGA GAAAACCAAA ATTCAATAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009142582.1
CDS	680..3232
Translation	MGNGTSRVVGCFVPSNDKNGVDSEFLEPLDEGLGHSFCYVRPSIFESPDITPSNSERFTIDSSTLDSETLSGSFRNEVVLDDPSFLNRHNSKGLGETTFKAISGASVSANVSTARTGNQTALCSSDVLEPAATFESTSSFASIPLQPLPRGGSGPLNGFMSGPLERGFASGPLDRNNGFMSGPIEKGVMSGPLDVSDKSNFSAPLSFRRRKPRFQRFMRSVSGPMRSTLARTFSRRSGGGLSWMQRFFLHPESRVSWPGGKEGKLHGEDHPESCLESNRHLQWAHGRAGEDRVHVVLSEEQGWLFIGIYDGFSGPDAPDFVMSHLYKAIDKELEGLLWVYDEPSEDNPLQPDQESHSTTQGNLLVPETISEQHSKSAQEGSEEVMTDNNSSLENADGPPGNLAGPGKKSTRLYELLQLERWDGEEVGLKHSVSDMTNQVEDASTSGGNDPCTTENTVLDEIPNSGQIHGTKKSQISSKIRRMYQKQKSLRKKLFPWSYDWHREEEGTTCVEEKIVESSGPIRRRWSGTVDHDDVLRAMARALESTEEAYMEMVEKSLDINPELALMGSCVLVMLMKDQDVYVMNVGDSRAILAQERLHDRHSNPGFGVEEGMGHKSRSRESLVRMELDRISEESPIHNLTTQISVSNKNRDVTSYRLKMRAVQLSSDHSTSVEEEVSRIRSEHPEDEQSILKDRVKGQLKVTRAFGAGFLKKPSFNEALLEMFRVDYIGTDPYISCEPCTVHHRLTSSDRFLVLCSDGLYEYFSNEEIVAHVTWFIENVPDGDPAQYLIAELLSRAATKNGMEFHDLLDIPQGDRRKYHDDVSVMVVSLEGRIWRSSGQYYPERKPKFNR

Target ORF information:

RefSeq Version	XM_009144334.2
Organism	Brassica rapa(field mustard)
Definition	Brassica rapa protein phosphatase 2C 32 (LOC103866418), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009144334.2

ORF Insert Sequence:

ATGGGAAACG GAACTTCCCG CGTTGTCGGC TGTTTCGTGC CGTCTAACGA CAAAAACGGC 
GTTGATTCGG AGTTTCTTGA GCCTCTAGAC GAAGGTTTAG GGCATTCCTT TTGCTACGTT 
AGGCCGAGCA TCTTCGAGTC TCCGGACATC ACTCCTTCTA ACTCGGAGAG GTTCACTATT 
GACTCAAGCA CGCTTGACTC TGAGACTCTC AGTGGATCAT TCCGAAACGA GGTCGTGCTT 
GATGATCCTT CGTTCTTGAA CAGACACAAC AGTAAAGGTT TGGGGGAGAC GACATTTAAG 
GCGATCTCAG GTGCTTCGGT TAGTGCCAAT GTGTCTACAG CTAGGACTGG GAATCAAACG 
GCGTTGTGTT CAAGTGACGT GTTGGAGCCT GCTGCTACAT TTGAGAGCAC GTCTTCTTTT 
GCTTCAATTC CTTTGCAACC ACTCCCTCGT GGTGGTTCCG GACCGTTGAA CGGGTTCATG 
TCAGGGCCTT TAGAGAGAGG TTTTGCGTCT GGTCCTTTGG ATAGAAACAA TGGTTTCATG 
TCAGGGCCTA TAGAGAAAGG AGTCATGTCT GGTCCTCTTG ATGTATCTGA TAAATCTAAT 
TTCTCGGCTC CGCTTTCTTT TAGACGTAGA AAGCCTCGGT TTCAGCGGTT TATGAGGAGT 
GTAAGCGGAC CAATGAGAAG TACATTAGCA AGAACATTCT CCAGAAGGTC TGGAGGTGGT 
TTAAGCTGGA TGCAACGCTT CTTCTTGCAT CCAGAGAGTA GAGTGTCTTG GCCTGGGGGG 
AAAGAAGGTA AATTACATGG TGAAGACCAC CCGGAGAGTT GTCTAGAGAG CAACCGTCAC 
TTGCAGTGGG CTCATGGGAG AGCTGGAGAA GACAGGGTTC ATGTTGTGCT TTCGGAGGAG 
CAAGGATGGC TCTTCATAGG GATATATGAT GGGTTTAGTG GACCGGATGC TCCGGACTTC 
GTGATGAGTC ATCTGTACAA AGCTATAGAC AAGGAACTGG AAGGTCTCCT CTGGGTTTAT 
GACGAGCCAT CTGAAGATAA TCCATTGCAG CCAGACCAGG AATCTCATTC TACTACACAG 
GGTAACTTGT TAGTTCCTGA AACTATCAGT GAGCAGCATT CAAAGTCAGC ACAAGAAGGA 
AGTGAAGAGG TTATGACTGA TAATAACAGT AGCCTTGAAA ATGCCGATGG TCCACCTGGA 
AATTTAGCAG GTCCTGGCAA GAAAAGCACG AGGCTTTACG AGCTGCTTCA GTTGGAACGG 
TGGGATGGAG AAGAAGTGGG ACTTAAACAT TCTGTAAGTG ATATGACCAA TCAGGTTGAA 
GATGCATCAA CTTCAGGTGG AAATGATCCG TGTACCACAG AGAACACCGT TCTTGATGAG 
ATTCCAAACT CAGGGCAAAT CCATGGGACT AAAAAATCAC AGATAAGCTC AAAGATAAGA 
AGAATGTATC AAAAACAAAA GTCTTTACGG AAAAAGCTGT TCCCATGGAG TTATGATTGG 
CACAGGGAAG AAGAAGGGAC GACTTGCGTT GAAGAGAAGA TCGTAGAGTC ATCAGGACCG 
ATTAGGAGAC GCTGGTCAGG AACCGTAGAC CATGATGATG TGCTAAGAGC AATGGCAAGA 
GCACTAGAAA GCACTGAGGA GGCTTACATG GAAATGGTGG AGAAGTCCCT TGACATAAAC 
CCGGAGCTTG CGTTAATGGG TTCATGTGTT CTCGTAATGC TAATGAAGGA TCAAGATGTT 
TACGTGATGA ATGTTGGTGA TAGTCGAGCT ATCTTGGCTC AGGAAAGGCT CCATGATCGT 
CATTCTAATC CAGGGTTTGG AGTTGAGGAA GGTATGGGGC ATAAGAGTAG GTCGAGAGAG 
TCGCTAGTGC GTATGGAACT AGATAGAATA TCAGAAGAAT CTCCAATACA CAATCTAACC 
ACTCAGATTA GTGTCTCAAA CAAAAACAGA GATGTAACTT CTTACCGGTT AAAGATGAGA 
GCTGTTCAAC TCTCAAGTGA CCATAGCACA AGTGTAGAAG AGGAAGTTTC AAGAATTAGA 
TCAGAACATC CAGAAGATGA GCAGTCCATA CTCAAAGACA GAGTCAAAGG CCAGCTCAAA 
GTCACTCGAG CTTTTGGTGC TGGTTTTCTC AAAAAGCCGA GTTTCAATGA AGCTTTGCTT 
GAGATGTTCA GAGTAGACTA CATTGGAACA GATCCGTATA TCAGCTGCGA ACCGTGTACT 
GTCCATCACA GGCTCACTTC AAGCGATCGG TTCTTGGTTT TATGCTCTGA TGGATTGTAT 
GAATACTTCA GCAACGAAGA GATTGTTGCT CACGTGACTT GGTTCATTGA AAACGTTCCT 
GATGGTGATC CTGCTCAATA CCTCATTGCA GAGCTTCTCT CTCGTGCAGC CACCAAGAAC 
GGGATGGAGT TTCATGATCT GTTGGATATA CCTCAAGGAG ATAGAAGGAA GTACCATGAT 
GATGTATCTG TGATGGTTGT GTCTCTTGAA GGAAGGATTT GGCGATCGTC TGGACAATAT 
TATCCTGAGA GAAAACCAAA ATTCAATAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103866418 ( XM_009144333.2 ) cDNA ORF clone, Brassica rapa(field mustard) -> XP_009142581.1 Brassica rapa protein phosphatase 2C 32 (LOC103866418), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBr149949
Clone ID Related Accession (Same CDS sequence)	XM_009144334.2 , XM_009144333.2
Accession Version	XM_009144333.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-12
Organism	Brassica rapa(field mustard)
Product	protein phosphatase 2C 32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024799.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 13, 2016 this sequence version replaced XM_009144333.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brassica rapa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACG GAACTTCCCG CGTTGTCGGC TGTTTCGTGC CGTCTAACGA CAAAAACGGC 
GTTGATTCGG AGTTTCTTGA GCCTCTAGAC GAAGGTTTAG GGCATTCCTT TTGCTACGTT 
AGGCCGAGCA TCTTCGAGTC TCCGGACATC ACTCCTTCTA ACTCGGAGAG GTTCACTATT 
GACTCAAGCA CGCTTGACTC TGAGACTCTC AGTGGATCAT TCCGAAACGA GGTCGTGCTT 
GATGATCCTT CGTTCTTGAA CAGACACAAC AGTAAAGGTT TGGGGGAGAC GACATTTAAG 
GCGATCTCAG GTGCTTCGGT TAGTGCCAAT GTGTCTACAG CTAGGACTGG GAATCAAACG 
GCGTTGTGTT CAAGTGACGT GTTGGAGCCT GCTGCTACAT TTGAGAGCAC GTCTTCTTTT 
GCTTCAATTC CTTTGCAACC ACTCCCTCGT GGTGGTTCCG GACCGTTGAA CGGGTTCATG 
TCAGGGCCTT TAGAGAGAGG TTTTGCGTCT GGTCCTTTGG ATAGAAACAA TGGTTTCATG 
TCAGGGCCTA TAGAGAAAGG AGTCATGTCT GGTCCTCTTG ATGTATCTGA TAAATCTAAT 
TTCTCGGCTC CGCTTTCTTT TAGACGTAGA AAGCCTCGGT TTCAGCGGTT TATGAGGAGT 
GTAAGCGGAC CAATGAGAAG TACATTAGCA AGAACATTCT CCAGAAGGTC TGGAGGTGGT 
TTAAGCTGGA TGCAACGCTT CTTCTTGCAT CCAGAGAGTA GAGTGTCTTG GCCTGGGGGG 
AAAGAAGGTA AATTACATGG TGAAGACCAC CCGGAGAGTT GTCTAGAGAG CAACCGTCAC 
TTGCAGTGGG CTCATGGGAG AGCTGGAGAA GACAGGGTTC ATGTTGTGCT TTCGGAGGAG 
CAAGGATGGC TCTTCATAGG GATATATGAT GGGTTTAGTG GACCGGATGC TCCGGACTTC 
GTGATGAGTC ATCTGTACAA AGCTATAGAC AAGGAACTGG AAGGTCTCCT CTGGGTTTAT 
GACGAGCCAT CTGAAGATAA TCCATTGCAG CCAGACCAGG AATCTCATTC TACTACACAG 
GGTAACTTGT TAGTTCCTGA AACTATCAGT GAGCAGCATT CAAAGTCAGC ACAAGAAGGA 
AGTGAAGAGG TTATGACTGA TAATAACAGT AGCCTTGAAA ATGCCGATGG TCCACCTGGA 
AATTTAGCAG GTCCTGGCAA GAAAAGCACG AGGCTTTACG AGCTGCTTCA GTTGGAACGG 
TGGGATGGAG AAGAAGTGGG ACTTAAACAT TCTGTAAGTG ATATGACCAA TCAGGTTGAA 
GATGCATCAA CTTCAGGTGG AAATGATCCG TGTACCACAG AGAACACCGT TCTTGATGAG 
ATTCCAAACT CAGGGCAAAT CCATGGGACT AAAAAATCAC AGATAAGCTC AAAGATAAGA 
AGAATGTATC AAAAACAAAA GTCTTTACGG AAAAAGCTGT TCCCATGGAG TTATGATTGG 
CACAGGGAAG AAGAAGGGAC GACTTGCGTT GAAGAGAAGA TCGTAGAGTC ATCAGGACCG 
ATTAGGAGAC GCTGGTCAGG AACCGTAGAC CATGATGATG TGCTAAGAGC AATGGCAAGA 
GCACTAGAAA GCACTGAGGA GGCTTACATG GAAATGGTGG AGAAGTCCCT TGACATAAAC 
CCGGAGCTTG CGTTAATGGG TTCATGTGTT CTCGTAATGC TAATGAAGGA TCAAGATGTT 
TACGTGATGA ATGTTGGTGA TAGTCGAGCT ATCTTGGCTC AGGAAAGGCT CCATGATCGT 
CATTCTAATC CAGGGTTTGG AGTTGAGGAA GGTATGGGGC ATAAGAGTAG GTCGAGAGAG 
TCGCTAGTGC GTATGGAACT AGATAGAATA TCAGAAGAAT CTCCAATACA CAATCTAACC 
ACTCAGATTA GTGTCTCAAA CAAAAACAGA GATGTAACTT CTTACCGGTT AAAGATGAGA 
GCTGTTCAAC TCTCAAGTGA CCATAGCACA AGTGTAGAAG AGGAAGTTTC AAGAATTAGA 
TCAGAACATC CAGAAGATGA GCAGTCCATA CTCAAAGACA GAGTCAAAGG CCAGCTCAAA 
GTCACTCGAG CTTTTGGTGC TGGTTTTCTC AAAAAGCCGA GTTTCAATGA AGCTTTGCTT 
GAGATGTTCA GAGTAGACTA CATTGGAACA GATCCGTATA TCAGCTGCGA ACCGTGTACT 
GTCCATCACA GGCTCACTTC AAGCGATCGG TTCTTGGTTT TATGCTCTGA TGGATTGTAT 
GAATACTTCA GCAACGAAGA GATTGTTGCT CACGTGACTT GGTTCATTGA AAACGTTCCT 
GATGGTGATC CTGCTCAATA CCTCATTGCA GAGCTTCTCT CTCGTGCAGC CACCAAGAAC 
GGGATGGAGT TTCATGATCT GTTGGATATA CCTCAAGGAG ATAGAAGGAA GTACCATGAT 
GATGTATCTG TGATGGTTGT GTCTCTTGAA GGAAGGATTT GGCGATCGTC TGGACAATAT 
TATCCTGAGA GAAAACCAAA ATTCAATAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009142581.1
CDS	760..3312
Translation	MGNGTSRVVGCFVPSNDKNGVDSEFLEPLDEGLGHSFCYVRPSIFESPDITPSNSERFTIDSSTLDSETLSGSFRNEVVLDDPSFLNRHNSKGLGETTFKAISGASVSANVSTARTGNQTALCSSDVLEPAATFESTSSFASIPLQPLPRGGSGPLNGFMSGPLERGFASGPLDRNNGFMSGPIEKGVMSGPLDVSDKSNFSAPLSFRRRKPRFQRFMRSVSGPMRSTLARTFSRRSGGGLSWMQRFFLHPESRVSWPGGKEGKLHGEDHPESCLESNRHLQWAHGRAGEDRVHVVLSEEQGWLFIGIYDGFSGPDAPDFVMSHLYKAIDKELEGLLWVYDEPSEDNPLQPDQESHSTTQGNLLVPETISEQHSKSAQEGSEEVMTDNNSSLENADGPPGNLAGPGKKSTRLYELLQLERWDGEEVGLKHSVSDMTNQVEDASTSGGNDPCTTENTVLDEIPNSGQIHGTKKSQISSKIRRMYQKQKSLRKKLFPWSYDWHREEEGTTCVEEKIVESSGPIRRRWSGTVDHDDVLRAMARALESTEEAYMEMVEKSLDINPELALMGSCVLVMLMKDQDVYVMNVGDSRAILAQERLHDRHSNPGFGVEEGMGHKSRSRESLVRMELDRISEESPIHNLTTQISVSNKNRDVTSYRLKMRAVQLSSDHSTSVEEEVSRIRSEHPEDEQSILKDRVKGQLKVTRAFGAGFLKKPSFNEALLEMFRVDYIGTDPYISCEPCTVHHRLTSSDRFLVLCSDGLYEYFSNEEIVAHVTWFIENVPDGDPAQYLIAELLSRAATKNGMEFHDLLDIPQGDRRKYHDDVSVMVVSLEGRIWRSSGQYYPERKPKFNR

Target ORF information:

RefSeq Version	XM_009144333.2
Organism	Brassica rapa(field mustard)
Definition	Brassica rapa protein phosphatase 2C 32 (LOC103866418), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009144333.2

ORF Insert Sequence:

ATGGGAAACG GAACTTCCCG CGTTGTCGGC TGTTTCGTGC CGTCTAACGA CAAAAACGGC 
GTTGATTCGG AGTTTCTTGA GCCTCTAGAC GAAGGTTTAG GGCATTCCTT TTGCTACGTT 
AGGCCGAGCA TCTTCGAGTC TCCGGACATC ACTCCTTCTA ACTCGGAGAG GTTCACTATT 
GACTCAAGCA CGCTTGACTC TGAGACTCTC AGTGGATCAT TCCGAAACGA GGTCGTGCTT 
GATGATCCTT CGTTCTTGAA CAGACACAAC AGTAAAGGTT TGGGGGAGAC GACATTTAAG 
GCGATCTCAG GTGCTTCGGT TAGTGCCAAT GTGTCTACAG CTAGGACTGG GAATCAAACG 
GCGTTGTGTT CAAGTGACGT GTTGGAGCCT GCTGCTACAT TTGAGAGCAC GTCTTCTTTT 
GCTTCAATTC CTTTGCAACC ACTCCCTCGT GGTGGTTCCG GACCGTTGAA CGGGTTCATG 
TCAGGGCCTT TAGAGAGAGG TTTTGCGTCT GGTCCTTTGG ATAGAAACAA TGGTTTCATG 
TCAGGGCCTA TAGAGAAAGG AGTCATGTCT GGTCCTCTTG ATGTATCTGA TAAATCTAAT 
TTCTCGGCTC CGCTTTCTTT TAGACGTAGA AAGCCTCGGT TTCAGCGGTT TATGAGGAGT 
GTAAGCGGAC CAATGAGAAG TACATTAGCA AGAACATTCT CCAGAAGGTC TGGAGGTGGT 
TTAAGCTGGA TGCAACGCTT CTTCTTGCAT CCAGAGAGTA GAGTGTCTTG GCCTGGGGGG 
AAAGAAGGTA AATTACATGG TGAAGACCAC CCGGAGAGTT GTCTAGAGAG CAACCGTCAC 
TTGCAGTGGG CTCATGGGAG AGCTGGAGAA GACAGGGTTC ATGTTGTGCT TTCGGAGGAG 
CAAGGATGGC TCTTCATAGG GATATATGAT GGGTTTAGTG GACCGGATGC TCCGGACTTC 
GTGATGAGTC ATCTGTACAA AGCTATAGAC AAGGAACTGG AAGGTCTCCT CTGGGTTTAT 
GACGAGCCAT CTGAAGATAA TCCATTGCAG CCAGACCAGG AATCTCATTC TACTACACAG 
GGTAACTTGT TAGTTCCTGA AACTATCAGT GAGCAGCATT CAAAGTCAGC ACAAGAAGGA 
AGTGAAGAGG TTATGACTGA TAATAACAGT AGCCTTGAAA ATGCCGATGG TCCACCTGGA 
AATTTAGCAG GTCCTGGCAA GAAAAGCACG AGGCTTTACG AGCTGCTTCA GTTGGAACGG 
TGGGATGGAG AAGAAGTGGG ACTTAAACAT TCTGTAAGTG ATATGACCAA TCAGGTTGAA 
GATGCATCAA CTTCAGGTGG AAATGATCCG TGTACCACAG AGAACACCGT TCTTGATGAG 
ATTCCAAACT CAGGGCAAAT CCATGGGACT AAAAAATCAC AGATAAGCTC AAAGATAAGA 
AGAATGTATC AAAAACAAAA GTCTTTACGG AAAAAGCTGT TCCCATGGAG TTATGATTGG 
CACAGGGAAG AAGAAGGGAC GACTTGCGTT GAAGAGAAGA TCGTAGAGTC ATCAGGACCG 
ATTAGGAGAC GCTGGTCAGG AACCGTAGAC CATGATGATG TGCTAAGAGC AATGGCAAGA 
GCACTAGAAA GCACTGAGGA GGCTTACATG GAAATGGTGG AGAAGTCCCT TGACATAAAC 
CCGGAGCTTG CGTTAATGGG TTCATGTGTT CTCGTAATGC TAATGAAGGA TCAAGATGTT 
TACGTGATGA ATGTTGGTGA TAGTCGAGCT ATCTTGGCTC AGGAAAGGCT CCATGATCGT 
CATTCTAATC CAGGGTTTGG AGTTGAGGAA GGTATGGGGC ATAAGAGTAG GTCGAGAGAG 
TCGCTAGTGC GTATGGAACT AGATAGAATA TCAGAAGAAT CTCCAATACA CAATCTAACC 
ACTCAGATTA GTGTCTCAAA CAAAAACAGA GATGTAACTT CTTACCGGTT AAAGATGAGA 
GCTGTTCAAC TCTCAAGTGA CCATAGCACA AGTGTAGAAG AGGAAGTTTC AAGAATTAGA 
TCAGAACATC CAGAAGATGA GCAGTCCATA CTCAAAGACA GAGTCAAAGG CCAGCTCAAA 
GTCACTCGAG CTTTTGGTGC TGGTTTTCTC AAAAAGCCGA GTTTCAATGA AGCTTTGCTT 
GAGATGTTCA GAGTAGACTA CATTGGAACA GATCCGTATA TCAGCTGCGA ACCGTGTACT 
GTCCATCACA GGCTCACTTC AAGCGATCGG TTCTTGGTTT TATGCTCTGA TGGATTGTAT 
GAATACTTCA GCAACGAAGA GATTGTTGCT CACGTGACTT GGTTCATTGA AAACGTTCCT 
GATGGTGATC CTGCTCAATA CCTCATTGCA GAGCTTCTCT CTCGTGCAGC CACCAAGAAC 
GGGATGGAGT TTCATGATCT GTTGGATATA CCTCAAGGAG ATAGAAGGAA GTACCATGAT 
GATGTATCTG TGATGGTTGT GTCTCTTGAA GGAAGGATTT GGCGATCGTC TGGACAATAT 
TATCCTGAGA GAAAACCAAA ATTCAATAGA TGA