LOC103864357 cDNA ORF clone, Brassica rapa(field mustard)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103864357
Entrez Gene ID	103864357
Full Name	putative nuclease HARBI1
Gene Type	protein-coding
Organism	Brassica rapa(field mustard)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009142112.2	XP_009140360.1	putative nuclease HARBI1

Pathways from BioSystems
Gene Ontology

The following LOC103864357 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103864357 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr145684	XM_009142112.2 Latest version!	Brassica rapa putative nuclease HARBI1 (LOC103864357), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103864357 ( XM_009142112.2 ) cDNA ORF clone, Brassica rapa(field mustard) -> XP_009140360.1 Brassica rapa putative nuclease HARBI1 (LOC103864357), mRNA.

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CloneID	OBr145684
Clone ID Related Accession (Same CDS sequence)	XM_009142112.2
Accession Version	XM_009142112.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-12
Organism	Brassica rapa(field mustard)
Product	putative nuclease HARBI1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024798.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 13, 2016 this sequence version replaced XM_009142112.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brassica rapa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTT CTTCTTCTGA TAATTTTGAC GACTTTATGG ATGAAAAGTT CGATCAAATT 
TTCGATCAAC AATTCGATAA TCTTCTCATT CGAGCTGAAA ATCGTCAAGA AGCTTCAAGA 
TCAAAAAAGA AACGAGCTTA CATTGAAAGA CAACGAGAAC AAGGGCACTT ACAGTTATGG 
AACGACTATT TTAGTGAAGA TGCAACATAT CCTTCTCACA TGTTTCGACG CCGTTTTAGA 
ATGAACAAGT CGTTGTTCAT GCGAATCACT GATCGACTCT CCGCTGAAAT CCCATACTTT 
CAACAGAGAA GAGATGCTAC TGGAAGGTTA GGTCTCTCTC CGCTACAAAA GGCAACGGCA 
GCAATTCGTA TGATGGCATA TGGTTGCCCA GCTGATGCGG TCGACGAATA CCTCCGACTT 
GGTGAGACTA CTGCGCTTTT ATGCTTAGAA CATTTTGTTC AAGGAATCAT AGATTTATTT 
GGAGCTGAGT ATCTAAGAAC CCCCACTCCA GAAGATCTTC AACGACTACT CGATATTGGA 
GAGATACGCG GATTTCCCGG GATGATAGGA AGCATTGATT GTATGCACTG GGAGTGGAAG 
AATTGCCCGA CCGCATGGAA AGGACAATAT ACACGGGGGT CCGGAAAGCC AACAATTGTT 
CTTGAGGCTG TAGCTTCACA AGACCTCTGG ATATGGCACG CGTTTTTCGG CCCTCCAGGT 
ACATTAAACG ATATCAATGT TCTTGAACGA TCACCAGTTT TTGATGATAT ATTACAAGGT 
CGAGCTCCGA AAGTTAACTA CACTGTCAAC GGACACGAGT ACCGTTTGGC TTACTATCTC 
ACAGATGGTA TTTATCCAAA ATGGGCTACG TTTGTCCAAT CTATTCCACT TCCGCAAGGT 
CCAAAAGCAT CTTTATTCGC TACATATCAA GAAGCTGTAC GTAAAGATGT CGAGCGAGCT 
TTTGGGGTCT TGCAAGCTCG GTTTGCTATA GTCAAAAATC CAGCTCTTTT ATGGGATAAA 
ATAAAAATTG GAAAGATAAT GAGAGCTTGT ATCATTCTAC ATAATATGAT CGTCGAAGAC 
GAACGAGATG GGTACACCCA GTTTGATCTT TCGATTTTCG AAACATCGGA TTCAAACCGA 
AGTTCAGAAG TGGATCTCTC GTTTTCTACA GATATGCCTT CAAATCTCGG CAATATGATG 
AGCATTCGGA ATCAAGTTCG TGATCGAACA ATACATCAAC GATTGAAAGC GGATTTGGTT 
GAGCATATAT GGCAAAAATT TGGAACAAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009140360.1
CDS	536..1828
Translation	MDASSSDNFDDFMDEKFDQIFDQQFDNLLIRAENRQEASRSKKKRAYIERQREQGHLQLWNDYFSEDATYPSHMFRRRFRMNKSLFMRITDRLSAEIPYFQQRRDATGRLGLSPLQKATAAIRMMAYGCPADAVDEYLRLGETTALLCLEHFVQGIIDLFGAEYLRTPTPEDLQRLLDIGEIRGFPGMIGSIDCMHWEWKNCPTAWKGQYTRGSGKPTIVLEAVASQDLWIWHAFFGPPGTLNDINVLERSPVFDDILQGRAPKVNYTVNGHEYRLAYYLTDGIYPKWATFVQSIPLPQGPKASLFATYQEAVRKDVERAFGVLQARFAIVKNPALLWDKIKIGKIMRACIILHNMIVEDERDGYTQFDLSIFETSDSNRSSEVDLSFSTDMPSNLGNMMSIRNQVRDRTIHQRLKADLVEHIWQKFGTN

Target ORF information:

RefSeq Version	XM_009142112.2
Organism	Brassica rapa(field mustard)
Definition	Brassica rapa putative nuclease HARBI1 (LOC103864357), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009142112.2

ORF Insert Sequence:

ATGGATGCTT CTTCTTCTGA TAATTTTGAC GACTTTATGG ATGAAAAGTT CGATCAAATT 
TTCGATCAAC AATTCGATAA TCTTCTCATT CGAGCTGAAA ATCGTCAAGA AGCTTCAAGA 
TCAAAAAAGA AACGAGCTTA CATTGAAAGA CAACGAGAAC AAGGGCACTT ACAGTTATGG 
AACGACTATT TTAGTGAAGA TGCAACATAT CCTTCTCACA TGTTTCGACG CCGTTTTAGA 
ATGAACAAGT CGTTGTTCAT GCGAATCACT GATCGACTCT CCGCTGAAAT CCCATACTTT 
CAACAGAGAA GAGATGCTAC TGGAAGGTTA GGTCTCTCTC CGCTACAAAA GGCAACGGCA 
GCAATTCGTA TGATGGCATA TGGTTGCCCA GCTGATGCGG TCGACGAATA CCTCCGACTT 
GGTGAGACTA CTGCGCTTTT ATGCTTAGAA CATTTTGTTC AAGGAATCAT AGATTTATTT 
GGAGCTGAGT ATCTAAGAAC CCCCACTCCA GAAGATCTTC AACGACTACT CGATATTGGA 
GAGATACGCG GATTTCCCGG GATGATAGGA AGCATTGATT GTATGCACTG GGAGTGGAAG 
AATTGCCCGA CCGCATGGAA AGGACAATAT ACACGGGGGT CCGGAAAGCC AACAATTGTT 
CTTGAGGCTG TAGCTTCACA AGACCTCTGG ATATGGCACG CGTTTTTCGG CCCTCCAGGT 
ACATTAAACG ATATCAATGT TCTTGAACGA TCACCAGTTT TTGATGATAT ATTACAAGGT 
CGAGCTCCGA AAGTTAACTA CACTGTCAAC GGACACGAGT ACCGTTTGGC TTACTATCTC 
ACAGATGGTA TTTATCCAAA ATGGGCTACG TTTGTCCAAT CTATTCCACT TCCGCAAGGT 
CCAAAAGCAT CTTTATTCGC TACATATCAA GAAGCTGTAC GTAAAGATGT CGAGCGAGCT 
TTTGGGGTCT TGCAAGCTCG GTTTGCTATA GTCAAAAATC CAGCTCTTTT ATGGGATAAA 
ATAAAAATTG GAAAGATAAT GAGAGCTTGT ATCATTCTAC ATAATATGAT CGTCGAAGAC 
GAACGAGATG GGTACACCCA GTTTGATCTT TCGATTTTCG AAACATCGGA TTCAAACCGA 
AGTTCAGAAG TGGATCTCTC GTTTTCTACA GATATGCCTT CAAATCTCGG CAATATGATG 
AGCATTCGGA ATCAAGTTCG TGATCGAACA ATACATCAAC GATTGAAAGC GGATTTGGTT 
GAGCATATAT GGCAAAAATT TGGAACAAAT TAA