LOC103849994 cDNA ORF clone, Brassica rapa(field mustard)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103849994
Entrez Gene ID	103849994
Full Name	probable ATP-dependent DNA helicase CHR12
Gene Type	protein-coding
Organism	Brassica rapa(field mustard)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009126695.2	XP_009124943.1	probable ATP-dependent DNA helicase CHR12

Pathways from BioSystems
Gene Ontology

The following LOC103849994 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103849994 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr129782	XM_009126695.2 Latest version!	Brassica rapa probable ATP-dependent DNA helicase CHR12 (LOC103849994), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103849994 ( XM_009126695.2 ) cDNA ORF clone, Brassica rapa(field mustard) -> XP_009124943.1 Brassica rapa probable ATP-dependent DNA helicase CHR12 (LOC103849994), mRNA.

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CloneID	OBr129782
Clone ID Related Accession (Same CDS sequence)	XM_009126695.2
Accession Version	XM_009126695.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-12
Organism	Brassica rapa(field mustard)
Product	probable ATP-dependent DNA helicase CHR12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024795.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 13, 2016 this sequence version replaced XM_009126695.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brassica rapa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCTC AGCAGCTAGG AGAGAGCAGC AGCGTCGTCG TCGTCGAATC TCGGGAAGAG 
CCTGTTGAGA CGACCAAGTC TCTGATCTGT GCTCTCAATT ACATCTCTCG CGACCTTCCT 
TTGCCTCCTC ACCTCTTCGC CGCCGTCTCT TCTATCTACC ATGGCTCCTC CTCCTCCTCT 
TCTCTCCCTC CCTCCGTCGT GCCTCCGCCG CCGGGGAATG ATTTTACTCC TTATGGAGGT 
GATTTGATGG GAGAGTTTGA GGATGCACTT TTGAAGCAGA GAACGAACAT TGAATCAGGC 
TCTCGATTGA CAGAATTGCA GGATAATCGG AACAAGACTC TTATTCAGCG CCGCTTATCC 
GAGCTTGAAG AATTACCTTC CACCAGAGGT GAAGACTTGC AAGGGAAGTG CTTACTCGAG 
CTTTATGGGC TAAAGCTACG AGAGTTGCAA AGCAAGGTTC GAACAGAGGT GAGCTCGGAG 
TTCTGGCTAC GTATGAACTG TGCTGATGTC AGTAGCCAAT TGTTTGACTG GGGTATGATG 
CGGTTGCCTC GTCTTTGGTA TGGTATTGGA GATCCTTTTG CTACGGAAGC TGATGATCAG 
TTCAGAAAGA AACGTGATGC CGAGAGGGTG TCACGTTTGG AAGAAGAGGA GAAGAATCTG 
ATTGAAACCG CCAAGAGGAA GTTCTTCGCA GAAGTGCTCA ATGCAGTTCG TGAGTTCCAG 
TTGCAAATCC AGGCGACTCA AAAACGCCGC AGGCAAAGAA ACGATGGTGT GCAGGCATGG 
CATGGGAGAC AAAGACAACG TGCAACCCGC GCTGAAAAGC TGAGGCTAAT GGCCCTTAAA 
TCCGATGACC AAGAAGCATA CATGAAACTA GTAAAGGAGA GCAAAAACGA AAGGCTCACC 
ACTCTTCTAG AAGAGACTAA CAAACTCCTT ACTAATTTGG GAGCTGCTGT TCAGCGGCAG 
AAAGATGCTA AACTACCAGA CGGCATTGAT CCACTGAAAG ACTCGGAATC TGATTTATCT 
GAACTCGATG CTCCGAAAGG CGAACCGTTA CAGGATTTGC TTCCTGATCA GGATATTGAC 
GTCACTGAAT CTGATAATAA TGATGATTCC AATGATTTAC TTGAAGGCCA GAGGCAGTAT 
AACTCTGCTA TCCATTCAAT TCAAGAGAAG GTGACGGAGC AGCCTTCACT TCTAAAAGGT 
GGGGAACTAA GATCTTACCA AATAGAAGGA CTCCAGTGGA TGGTTTCTCT ATTCAACAAC 
AACCTTAATG GGATTTTAGC TGATGAGATG GGTTTGGGGA AAACCATCCA AACAATCTCG 
TTGATTGCTT ATCTTCTGGA GAATAAAGGT GTGCCTGGTC CTTATCTGAT AGTGGCTCCA 
AAAGCTGTGC TACCAAACTG GGTAAACGAG TTTGCTTTAT GGGTACCAAG CATTGCAGCT 
TATCTTTATG ATGGACGTTT GGAGGAAAGA AAGGCAATCA GGGAGAAAAT TGCAGGAGAA 
GGGAAGTTCA ACGTGTTGAT AACCCACTAT GATCTCATCA TGAGAGATAA AGCATTTTTG 
AAGAAAATTG ATTGGCACTA CATGATTGTT GATGAAGGAC ATCGTCTGAA GAACCATGAA 
TCTGCTCTTG CAAAGACTCT ACTAACAGGC TATCGGATAA AACGCAGACT CCTGTTAACG 
GGAACACCCA TACAGAACAG CCTTCAAGAA CTATGGTCAC TACTCAATTT TCTCCTTCCT 
CACATCTTTA ACTCGGTTCA GAACTTTGAG GAATGGTTTA ATGCTCCGTT TGCTGATCGT 
GCTAACGTTA GTCTTACTGA TGAAGAGGAG CTGTTGGTTA TCCACCGTCT GCATCATGTT 
ATAAGACCGT TTATACTGAG AAGGAAAAAG GACGAAGTAG AGAAGTTCCT TCCTGGAAAG 
ACACAGGTGA TACTGAAGTG TGACATGTCA GCATGGCAGA AAGTGTATTA CAAACAAGTT 
ACGGACATGG GCAGAGTTGG CCTTCAAACA GGATCCGGGA AGTCAAAGAG TCTGCAAAAT 
CTAACGATGC AACTGAGAAA GTGTTGTAAC CATCCGTACC TATTCGTTGG AGGAGAGTAC 
AACATGTGGA AGAAGCCTGA GATTGTGAGA GCTTCAGGGA AGTTCGAGCT CCTCGACCGT 
CTCCTTCCGA AGCTGCGCAA GGCCGGGCAT AGAATCCTGC TCTTCTCTCA GATGACTCGT 
CTCATTGACA TCCTCGAGAT TTATCTGACG CTTAACGATT TCAAGTACCT AAGACTTGAC 
GGTACCACAA AGACGGATCA AAGAGGGCTT TTGCTGAAGC AGTTCAACGA GCCGGACTCT 
CCTTACTTCA TGTTTCTTCT GAGCACGCGC GCTGGAGGTC TCGGTCTGAA CTTGCAGACT 
GCAGACACTG TTATTATCTT TGACAGTGAT TGGAACCCAC AGATGGACCA ACAGGCTGAG 
GATAGAGCTC ATAGGATAGG GCAGAAGAAG GAAGTGAGAG TGTTTGTTTT GGTTAGCGTT 
GGCTCCGTTG AAGAAGTGAT ATTGGAGCGT GCAAAGCAGA AGATGGGGAT TGATGCTAAA 
GTCATACAAG CTGGTCTTTT CAACACAACT TCCACAGCAC AAGACAGAAG AGAGATGCTT 
GAAGAGCTCA TGCGCAAAGG AACAAGCTCA CTAGGGAACG ATGTCCCCAG TGAGAGGGAA 
ATAAACCGGC TCGCGGCTCG TAGTGAGGAC GAGTTTTGGA TGTTTGAGAG AATGGACGAG 
GAGAGGAGAA TGAAGGAGAG TTACAGAACA CGTCTGATGC AAGAGCAGGA AGTCCCTGAG 
TGGGCATACA CCACACAGAG CCAAGATGAC AAGTCTAACA ACGCAAAGTA CCATTTTGGG 
AGTGTCACAG GCAAGCGAAA GCGTAAAGAG ATCGTTTATA GCGACTCATT GAGCGAGGTG 
CAGTGGATGA AAGCTGTGGA GAGCGGTGAA GACGTGTCAG CATACTCTCT TAAACAGAGG 
AGAGCGGAGA AGGCAAGCAA GGCAAAAACA TCAACGAGTA AAAGAGTGGT AGAACCTATC 
CAAGTGGTGA GTGATGAGAC GAGCGAGGAG GAAGAGGAGG AAGGAAGAGG AGGACAGGAG 
ATGAGTGGGA AACAGAGAGT AGAGAAGTCT GAAGAAGAAG AAGAAGAAGA TGGTGAAGAA 
GAGAATGATG AAAAGCCAAT ATTCAAGTGG AGTTCTCATA AGAAGAAAAG GTCGAGGTAC 
TCTTTTACGT GTTCTTCGTC TGATTCTAGA GCTCAAAGTT CCAATGGAAG TAGAAGAAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009124943.1
CDS	177..3479
Translation	MVAQQLGESSSVVVVESREEPVETTKSLICALNYISRDLPLPPHLFAAVSSIYHGSSSSSSLPPSVVPPPPGNDFTPYGGDLMGEFEDALLKQRTNIESGSRLTELQDNRNKTLIQRRLSELEELPSTRGEDLQGKCLLELYGLKLRELQSKVRTEVSSEFWLRMNCADVSSQLFDWGMMRLPRLWYGIGDPFATEADDQFRKKRDAERVSRLEEEEKNLIETAKRKFFAEVLNAVREFQLQIQATQKRRRQRNDGVQAWHGRQRQRATRAEKLRLMALKSDDQEAYMKLVKESKNERLTTLLEETNKLLTNLGAAVQRQKDAKLPDGIDPLKDSESDLSELDAPKGEPLQDLLPDQDIDVTESDNNDDSNDLLEGQRQYNSAIHSIQEKVTEQPSLLKGGELRSYQIEGLQWMVSLFNNNLNGILADEMGLGKTIQTISLIAYLLENKGVPGPYLIVAPKAVLPNWVNEFALWVPSIAAYLYDGRLEERKAIREKIAGEGKFNVLITHYDLIMRDKAFLKKIDWHYMIVDEGHRLKNHESALAKTLLTGYRIKRRLLLTGTPIQNSLQELWSLLNFLLPHIFNSVQNFEEWFNAPFADRANVSLTDEEELLVIHRLHHVIRPFILRRKKDEVEKFLPGKTQVILKCDMSAWQKVYYKQVTDMGRVGLQTGSGKSKSLQNLTMQLRKCCNHPYLFVGGEYNMWKKPEIVRASGKFELLDRLLPKLRKAGHRILLFSQMTRLIDILEIYLTLNDFKYLRLDGTTKTDQRGLLLKQFNEPDSPYFMFLLSTRAGGLGLNLQTADTVIIFDSDWNPQMDQQAEDRAHRIGQKKEVRVFVLVSVGSVEEVILERAKQKMGIDAKVIQAGLFNTTSTAQDRREMLEELMRKGTSSLGNDVPSEREINRLAARSEDEFWMFERMDEERRMKESYRTRLMQEQEVPEWAYTTQSQDDKSNNAKYHFGSVTGKRKRKEIVYSDSLSEVQWMKAVESGEDVSAYSLKQRRAEKASKAKTSTSKRVVEPIQVVSDETSEEEEEEGRGGQEMSGKQRVEKSEEEEEEDGEEENDEKPIFKWSSHKKKRSRYSFTCSSSDSRAQSSNGSRRN

Target ORF information:

RefSeq Version	XM_009126695.2
Organism	Brassica rapa(field mustard)
Definition	Brassica rapa probable ATP-dependent DNA helicase CHR12 (LOC103849994), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009126695.2

ORF Insert Sequence:

ATGGTGGCTC AGCAGCTAGG AGAGAGCAGC AGCGTCGTCG TCGTCGAATC TCGGGAAGAG 
CCTGTTGAGA CGACCAAGTC TCTGATCTGT GCTCTCAATT ACATCTCTCG CGACCTTCCT 
TTGCCTCCTC ACCTCTTCGC CGCCGTCTCT TCTATCTACC ATGGCTCCTC CTCCTCCTCT 
TCTCTCCCTC CCTCCGTCGT GCCTCCGCCG CCGGGGAATG ATTTTACTCC TTATGGAGGT 
GATTTGATGG GAGAGTTTGA GGATGCACTT TTGAAGCAGA GAACGAACAT TGAATCAGGC 
TCTCGATTGA CAGAATTGCA GGATAATCGG AACAAGACTC TTATTCAGCG CCGCTTATCC 
GAGCTTGAAG AATTACCTTC CACCAGAGGT GAAGACTTGC AAGGGAAGTG CTTACTCGAG 
CTTTATGGGC TAAAGCTACG AGAGTTGCAA AGCAAGGTTC GAACAGAGGT GAGCTCGGAG 
TTCTGGCTAC GTATGAACTG TGCTGATGTC AGTAGCCAAT TGTTTGACTG GGGTATGATG 
CGGTTGCCTC GTCTTTGGTA TGGTATTGGA GATCCTTTTG CTACGGAAGC TGATGATCAG 
TTCAGAAAGA AACGTGATGC CGAGAGGGTG TCACGTTTGG AAGAAGAGGA GAAGAATCTG 
ATTGAAACCG CCAAGAGGAA GTTCTTCGCA GAAGTGCTCA ATGCAGTTCG TGAGTTCCAG 
TTGCAAATCC AGGCGACTCA AAAACGCCGC AGGCAAAGAA ACGATGGTGT GCAGGCATGG 
CATGGGAGAC AAAGACAACG TGCAACCCGC GCTGAAAAGC TGAGGCTAAT GGCCCTTAAA 
TCCGATGACC AAGAAGCATA CATGAAACTA GTAAAGGAGA GCAAAAACGA AAGGCTCACC 
ACTCTTCTAG AAGAGACTAA CAAACTCCTT ACTAATTTGG GAGCTGCTGT TCAGCGGCAG 
AAAGATGCTA AACTACCAGA CGGCATTGAT CCACTGAAAG ACTCGGAATC TGATTTATCT 
GAACTCGATG CTCCGAAAGG CGAACCGTTA CAGGATTTGC TTCCTGATCA GGATATTGAC 
GTCACTGAAT CTGATAATAA TGATGATTCC AATGATTTAC TTGAAGGCCA GAGGCAGTAT 
AACTCTGCTA TCCATTCAAT TCAAGAGAAG GTGACGGAGC AGCCTTCACT TCTAAAAGGT 
GGGGAACTAA GATCTTACCA AATAGAAGGA CTCCAGTGGA TGGTTTCTCT ATTCAACAAC 
AACCTTAATG GGATTTTAGC TGATGAGATG GGTTTGGGGA AAACCATCCA AACAATCTCG 
TTGATTGCTT ATCTTCTGGA GAATAAAGGT GTGCCTGGTC CTTATCTGAT AGTGGCTCCA 
AAAGCTGTGC TACCAAACTG GGTAAACGAG TTTGCTTTAT GGGTACCAAG CATTGCAGCT 
TATCTTTATG ATGGACGTTT GGAGGAAAGA AAGGCAATCA GGGAGAAAAT TGCAGGAGAA 
GGGAAGTTCA ACGTGTTGAT AACCCACTAT GATCTCATCA TGAGAGATAA AGCATTTTTG 
AAGAAAATTG ATTGGCACTA CATGATTGTT GATGAAGGAC ATCGTCTGAA GAACCATGAA 
TCTGCTCTTG CAAAGACTCT ACTAACAGGC TATCGGATAA AACGCAGACT CCTGTTAACG 
GGAACACCCA TACAGAACAG CCTTCAAGAA CTATGGTCAC TACTCAATTT TCTCCTTCCT 
CACATCTTTA ACTCGGTTCA GAACTTTGAG GAATGGTTTA ATGCTCCGTT TGCTGATCGT 
GCTAACGTTA GTCTTACTGA TGAAGAGGAG CTGTTGGTTA TCCACCGTCT GCATCATGTT 
ATAAGACCGT TTATACTGAG AAGGAAAAAG GACGAAGTAG AGAAGTTCCT TCCTGGAAAG 
ACACAGGTGA TACTGAAGTG TGACATGTCA GCATGGCAGA AAGTGTATTA CAAACAAGTT 
ACGGACATGG GCAGAGTTGG CCTTCAAACA GGATCCGGGA AGTCAAAGAG TCTGCAAAAT 
CTAACGATGC AACTGAGAAA GTGTTGTAAC CATCCGTACC TATTCGTTGG AGGAGAGTAC 
AACATGTGGA AGAAGCCTGA GATTGTGAGA GCTTCAGGGA AGTTCGAGCT CCTCGACCGT 
CTCCTTCCGA AGCTGCGCAA GGCCGGGCAT AGAATCCTGC TCTTCTCTCA GATGACTCGT 
CTCATTGACA TCCTCGAGAT TTATCTGACG CTTAACGATT TCAAGTACCT AAGACTTGAC 
GGTACCACAA AGACGGATCA AAGAGGGCTT TTGCTGAAGC AGTTCAACGA GCCGGACTCT 
CCTTACTTCA TGTTTCTTCT GAGCACGCGC GCTGGAGGTC TCGGTCTGAA CTTGCAGACT 
GCAGACACTG TTATTATCTT TGACAGTGAT TGGAACCCAC AGATGGACCA ACAGGCTGAG 
GATAGAGCTC ATAGGATAGG GCAGAAGAAG GAAGTGAGAG TGTTTGTTTT GGTTAGCGTT 
GGCTCCGTTG AAGAAGTGAT ATTGGAGCGT GCAAAGCAGA AGATGGGGAT TGATGCTAAA 
GTCATACAAG CTGGTCTTTT CAACACAACT TCCACAGCAC AAGACAGAAG AGAGATGCTT 
GAAGAGCTCA TGCGCAAAGG AACAAGCTCA CTAGGGAACG ATGTCCCCAG TGAGAGGGAA 
ATAAACCGGC TCGCGGCTCG TAGTGAGGAC GAGTTTTGGA TGTTTGAGAG AATGGACGAG 
GAGAGGAGAA TGAAGGAGAG TTACAGAACA CGTCTGATGC AAGAGCAGGA AGTCCCTGAG 
TGGGCATACA CCACACAGAG CCAAGATGAC AAGTCTAACA ACGCAAAGTA CCATTTTGGG 
AGTGTCACAG GCAAGCGAAA GCGTAAAGAG ATCGTTTATA GCGACTCATT GAGCGAGGTG 
CAGTGGATGA AAGCTGTGGA GAGCGGTGAA GACGTGTCAG CATACTCTCT TAAACAGAGG 
AGAGCGGAGA AGGCAAGCAA GGCAAAAACA TCAACGAGTA AAAGAGTGGT AGAACCTATC 
CAAGTGGTGA GTGATGAGAC GAGCGAGGAG GAAGAGGAGG AAGGAAGAGG AGGACAGGAG 
ATGAGTGGGA AACAGAGAGT AGAGAAGTCT GAAGAAGAAG AAGAAGAAGA TGGTGAAGAA 
GAGAATGATG AAAAGCCAAT ATTCAAGTGG AGTTCTCATA AGAAGAAAAG GTCGAGGTAC 
TCTTTTACGT GTTCTTCGTC TGATTCTAGA GCTCAAAGTT CCAATGGAAG TAGAAGAAAC 
TGA