FLG cDNA ORF clone, Bos taurus(cattle)

The following FLG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FLG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa58346 XM_010803069.2
Latest version!
Bos taurus filaggrin (FLG), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa58346 XM_010826840.1
Latest version!
Bos taurus filaggrin (FLG), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa58346 XM_010826841.2
Latest version!
Bos taurus filaggrin (FLG), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa58348 XM_010803071.2
Latest version!
Bos taurus filaggrin (FLG), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa58348 XM_010826843.1
Latest version!
Bos taurus filaggrin (FLG), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa90409 XM_010803068.2
Latest version!
Bos taurus filaggrin (FLG), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $258.30
$369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa58346
    Clone ID Related Accession (Same CDS sequence) XM_010826841.2 , XM_010826840.1 , XM_010803069.2
    Accession Version XM_010826841.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product filaggrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644641.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826841.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCGACTC TCCTGGAAAA TATCAATGAC ATCATCAAAA TATTCCACAA ATATTCAAAA 
    ACAGATAAGG AGACTGACAC ATTGAGTGAA AAAGAGCTAA AGGAACTGGT GGAAGTGGAG
    TTTCGGCCCA TCCTGAAGAA TCCAGGTGAC CCGGACACCG CTGAAGTTTT CATGTATAAC
    CTCGATAGAG ATCACAACAA TAAAATAGAC TTTACAGAGT TTTTTCTGAT GGTATTCAAA
    GTGGCTCAAG TATATTATAG TTATACTCAA AGACAGAATT TACAGAGAGC AGGACAAAAA
    CAGAAAAAGT GTACATACCA CTACGGAGAT GAAGAAGATG ATACAGAAGA AGACAAAGAA
    GAAACAGAAA GAAAGTACAG TCATTCAAGA AGTGATGGAA AGACTCAAGA CAGATCAAAG
    AGCCCAAGAG GAAGAGGAAA AAAGAGACAT GGATCTAAAT CTGGAAGTAA ACAAAGAAGA
    GGTGACACAC CTACCTCTGG ACTCAGACAT GGATGTAGCA AAAAACATGA GTCAAGAAGG
    GAAAAGAAAA GGAGACCAAG CTCTACTGAA CCAAAAGAAA GAAGGCATAT GTCAAGTGTT
    AGCCCAACCA GGGGATATGA AGAAAAAGAA GAAGAACATG GTTATGAAAA TAAAGGTAAA
    ACAAGTGCAA AATGTATAGG ATCAGAGTAT GATGACTCCT ATCAAGTTTG TGAAGACAAA
    GTGACTACGA ATTTTCAACC CAGTCACAGT AAAAACTATG GGTCAAACAT CACGAAGGGC
    AGAGACACGG AAGGACATTC ACGAGATACA GGTAGGAAAT CCGTATTCAC TCATGCAAGA
    TCAGGGTCCA GTTCAAGGAA TCAAAATGGC TCTGTCCAGA CTCATACAGG AGACAATTCT
    ACCCACTCAG AATCCCAACA AGAGACAAAC AGTGAATCTG TCCACAGAAG GTCAAGAAAC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GACAACCCTC CACTGGATCC GGAGGCAGTA GACACAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GTAGACAGTC CGTGACAACT
    CATGGAAGGC CCGGTTCCAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGCACTCAGA GTCTCATCAA GGGAGGCACA GTGATTCTCT CCAAAGAAGG
    TCAGGTACTA GCACTGGGCA AAGACAGGGG AGCCACCACG AGCAGTCAAG AGACAGCTCC
    AGACACTCTG GAACTCAACA AGGACAAACC TCCACTGGAT CCGGAAGCAG TAGGCACAGA
    GACTCCAGTG TTAGTCAGGC CAGTGACAGT GAAGGACATT CAGAAGACTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ATCAACATGG ATCTTCCCAT
    GGCCAGACTG GAGACAGTTC TAGGCATTCA GAGTCCCATC AAAGAAGCCA CAGTGAAGCT
    GTTCATGAAA GGTCAGGATC CAGCACGGGG CAAAGGCAGA GGAGCCACCA TGAGCAGTCA
    AGGGACAGCT CCAGACACTC TGGAACTGGA CATGGACAAA TCTCAGCTGA ATCTGGAAAT
    GGCAGACATA GGGAATCCAG TGTTAGTCAA GCCAGTGACA GTGAGGGACA CGCCAGAGAT
    TCAGGTAGGC ATTCTGAGAC AACTCATGGA AGATATGGTT CCAGCTCAAG GAACCAACAT
    GGATCCTCCC ATGGTCAGAC AGGGGACAGT TATAGGCACT CAGAGACCAA TCAAGGGAGA
    CACAATAAGA GACACACAAG AGATTCAGGT AGGCATTCTG AGACAACTCA TGGAAGACCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCATGGCC AGAGTGGAGA CAGTTCTAGG
    CACTCAGAGT CCCATCAAGA GAGGCATAGT GAATCTGTCC ATGGAAGGTC AGGATCCAGC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGAG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GGCAAACCTC AGCTGAATCT GGAAATGGCA GACATAGGGA ATCCAGTGTT
    AGTCAAGCCA GTGACAGTGA GGGACACACC AGAGATTCAG GTAGGCATTC TGAGACAACT
    CATGGAAGGC CTGGTTCAAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGTACTCAGC ATATCATCAA GGGAGGCACA GTGAATCTGT CCATGAAAGG
    TCAGCGTCTA GAACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACAGTCTG GAACTGGACA TGGACATACC TCTGCTGGAT CTGGAAATGG CAGACATAGG
    GAATCCAGTG TTAGTCAGTC AAGTGACAGA GAGGGACACG CAAGAGATTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ACCAACATGT ATCTTCCCAT
    GGCCAGATAG GAGACAGTTC TAGCCACTCA GAGACCAATC AAGGGAGCCA CAATGAGAGA
    CATACAAGAG ATTCAGGTAG GCATTCCGAG ACAACTCATG GAAGACTTGG TTCCAGCTCA
    AGGAACCAAC ATGGATCTGT CCATGGCCAG ACAAGAGACA GTCCTAGGCA CTCAGAGTCC
    CATCAAGAGA GGCATAGTGA ATCTGTCCAT GGAAGGTCAG GATCCAGCAC TGGGCAAAGA
    CAGGAGAGCC ACCATGAGCA GTCAAGGGAC AGCTCCAGAC AGTCTGGAAC TGGACATGGA
    CATACCTCTG CTGGATCCGG AAATAGTAGG CACAGGGAAT CCAGTGTTAG TCAGGCCAGT
    GACAGTGAAG GACATTCAGA AGACTCAGGT AGACAGAACG TGACAACTCA TGGAAGGCCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCAGGGCC AGACAGGAGA CAGTTCTAGG
    TACTCAGCAT ATCATCAAGG GAGGCACAGT GAATCTGTCC ATGAAAGGTC AGCGTCTAGA
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACAGTCTGGA
    ACTGGACATG GACATGCCTC TGCTGGATCT GGAATCAACA GACATAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGAGA GGGACATGCA AGAGATTCAG GTAGACAGTC CGTGACAACT
    CATGGAAGGC CTGGTTCCAG CTCAAGAAAC CAACATGTAT CTTCCCATGG CCAGATAGGA
    GACAGTTCTA GCCACTCAGA GACCAATCAA GGGAGCCACA ATGAGAGACA TACAAGAGAT
    TCAGGTAGGC ATTCCGAGAC AACTCATGGA AGACTTGGTT CCAGCTCAAG GAACCAACAT
    GGATCTGTCC ATGGCCAGAC AAGAGACAGT CCTAGGCACT CAGAGTCCCA TCAAGAGAGG
    CACAGTGAAT CTGTCCATGA AAGGTCAGGA TCCAGCACTG GACAAAGACA GGGGAGCCAC
    CATGAGCAGT CAAGAGACAG CTCCAGACAC TCTGGAACTC GACATGGACA AACCTCTACT
    GTATCTGGAG GCAGTAGGCA CAGGGAACCC AGCGTTAGTC AGGCCAGTGA CAGTGAGGGA
    CATTCAGAAG ACTCAGGTAG ACAGTCCGTG ACAACTCATG GAAGGCCTGG TTCAACTTCA
    AGAAACCAAC ATGGATCTGT CCATGGCCAG ACAAGAGACA GTCCTAGGCA CTCAGAGTCC
    CATCAAGAGA GGCACAGTGA ATCTGTCCAC CAAAGGTCTG GATCAAGCAC AGGGCAAAGA
    CAGGGGAGCC ACCATGAGCA GTCAAGAGAC AGCTCCAGAC ACTCTGGAAC CGGTCATGGA
    CAAACCTCCG CTGGATCCGG AAGTAGTAGG CACAGGGAAT CCAGTGTTAG TCAGGCCAGT
    GACAGTGAAG GACATTCAGA AGACTCAGGT AGACAGAACG TGACAACTCA TGGAAGGCCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCAGGGCC AGACAGGAGA CAGTTCTAGG
    TACTCAGCAT CTCATCAAGG GAGGCACAGT GAATCTGTCC ATGAAAGGTC AACATCTAGA
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACAGTCTGGA
    ACTGGACATG GACATGCCTC TGCTGGATCT GGAATCAACA GACATAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GCAGACAGAA CGTGACAACT
    CATGGAAGGC CCGGTTCCAG CTCAAGCAAT CAACATGGAT CTTCCCATGG CCAGACTGGA
    GACAGTTCTA GGCACTCAGA GTCCCATCAA GGGAGCCACG GTGAATCTGT CCACCAAAGG
    TCTGGATCAA GCACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACACTCTG GAACTGGTCA TGGAGAAACA TCCACTGGAT CTGGAAGCAG CAGACATAGG
    GAATCCAGTG TTAGTCAGTC CAGTGACAGC AAGGGTCATT CAAGAGGTTC AGGTAATCAG
    ACTGTGACCA ACCAAAGAAT GTCTGCATTC TACTCAAGGT TCCAAAATCA TGGTGCAGGC
    CAAGTTTGGA GACATGGCAG CTATGGCAGT TCCAATTATG ACTATGGACA GTTAGGGACT
    GGACATTCTC CAGATGAAAA TTTTAGTCAC GATTCCAGTC ATGTGGAAAA GAGGGACAAA
    CCTGAATATA GAGGAGAACT AATGAGATCC AATATAACAG TCCGTAATAT ACACCCTGGA
    ACTTATGGCC ATTCAAATTA TATTTCAAAA CAGCTGGGAT TTGGACAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010825143.1
    CDS106..5076
    Translation

    Target ORF information:

    RefSeq Version XM_010826841.2
    Organism Bos taurus(cattle)
    Definition Bos taurus filaggrin (FLG), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826841.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCGACTC TCCTGGAAAA TATCAATGAC ATCATCAAAA TATTCCACAA ATATTCAAAA 
    ACAGATAAGG AGACTGACAC ATTGAGTGAA AAAGAGCTAA AGGAACTGGT GGAAGTGGAG
    TTTCGGCCCA TCCTGAAGAA TCCAGGTGAC CCGGACACCG CTGAAGTTTT CATGTATAAC
    CTCGATAGAG ATCACAACAA TAAAATAGAC TTTACAGAGT TTTTTCTGAT GGTATTCAAA
    GTGGCTCAAG TATATTATAG TTATACTCAA AGACAGAATT TACAGAGAGC AGGACAAAAA
    CAGAAAAAGT GTACATACCA CTACGGAGAT GAAGAAGATG ATACAGAAGA AGACAAAGAA
    GAAACAGAAA GAAAGTACAG TCATTCAAGA AGTGATGGAA AGACTCAAGA CAGATCAAAG
    AGCCCAAGAG GAAGAGGAAA AAAGAGACAT GGATCTAAAT CTGGAAGTAA ACAAAGAAGA
    GGTGACACAC CTACCTCTGG ACTCAGACAT GGATGTAGCA AAAAACATGA GTCAAGAAGG
    GAAAAGAAAA GGAGACCAAG CTCTACTGAA CCAAAAGAAA GAAGGCATAT GTCAAGTGTT
    AGCCCAACCA GGGGATATGA AGAAAAAGAA GAAGAACATG GTTATGAAAA TAAAGGTAAA
    ACAAGTGCAA AATGTATAGG ATCAGAGTAT GATGACTCCT ATCAAGTTTG TGAAGACAAA
    GTGACTACGA ATTTTCAACC CAGTCACAGT AAAAACTATG GGTCAAACAT CACGAAGGGC
    AGAGACACGG AAGGACATTC ACGAGATACA GGTAGGAAAT CCGTATTCAC TCATGCAAGA
    TCAGGGTCCA GTTCAAGGAA TCAAAATGGC TCTGTCCAGA CTCATACAGG AGACAATTCT
    ACCCACTCAG AATCCCAACA AGAGACAAAC AGTGAATCTG TCCACAGAAG GTCAAGAAAC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GACAACCCTC CACTGGATCC GGAGGCAGTA GACACAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GTAGACAGTC CGTGACAACT
    CATGGAAGGC CCGGTTCCAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGCACTCAGA GTCTCATCAA GGGAGGCACA GTGATTCTCT CCAAAGAAGG
    TCAGGTACTA GCACTGGGCA AAGACAGGGG AGCCACCACG AGCAGTCAAG AGACAGCTCC
    AGACACTCTG GAACTCAACA AGGACAAACC TCCACTGGAT CCGGAAGCAG TAGGCACAGA
    GACTCCAGTG TTAGTCAGGC CAGTGACAGT GAAGGACATT CAGAAGACTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ATCAACATGG ATCTTCCCAT
    GGCCAGACTG GAGACAGTTC TAGGCATTCA GAGTCCCATC AAAGAAGCCA CAGTGAAGCT
    GTTCATGAAA GGTCAGGATC CAGCACGGGG CAAAGGCAGA GGAGCCACCA TGAGCAGTCA
    AGGGACAGCT CCAGACACTC TGGAACTGGA CATGGACAAA TCTCAGCTGA ATCTGGAAAT
    GGCAGACATA GGGAATCCAG TGTTAGTCAA GCCAGTGACA GTGAGGGACA CGCCAGAGAT
    TCAGGTAGGC ATTCTGAGAC AACTCATGGA AGATATGGTT CCAGCTCAAG GAACCAACAT
    GGATCCTCCC ATGGTCAGAC AGGGGACAGT TATAGGCACT CAGAGACCAA TCAAGGGAGA
    CACAATAAGA GACACACAAG AGATTCAGGT AGGCATTCTG AGACAACTCA TGGAAGACCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCATGGCC AGAGTGGAGA CAGTTCTAGG
    CACTCAGAGT CCCATCAAGA GAGGCATAGT GAATCTGTCC ATGGAAGGTC AGGATCCAGC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGAG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GGCAAACCTC AGCTGAATCT GGAAATGGCA GACATAGGGA ATCCAGTGTT
    AGTCAAGCCA GTGACAGTGA GGGACACACC AGAGATTCAG GTAGGCATTC TGAGACAACT
    CATGGAAGGC CTGGTTCAAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGTACTCAGC ATATCATCAA GGGAGGCACA GTGAATCTGT CCATGAAAGG
    TCAGCGTCTA GAACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACAGTCTG GAACTGGACA TGGACATACC TCTGCTGGAT CTGGAAATGG CAGACATAGG
    GAATCCAGTG TTAGTCAGTC AAGTGACAGA GAGGGACACG CAAGAGATTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ACCAACATGT ATCTTCCCAT
    GGCCAGATAG GAGACAGTTC TAGCCACTCA GAGACCAATC AAGGGAGCCA CAATGAGAGA
    CATACAAGAG ATTCAGGTAG GCATTCCGAG ACAACTCATG GAAGACTTGG TTCCAGCTCA
    AGGAACCAAC ATGGATCTGT CCATGGCCAG ACAAGAGACA GTCCTAGGCA CTCAGAGTCC
    CATCAAGAGA GGCATAGTGA ATCTGTCCAT GGAAGGTCAG GATCCAGCAC TGGGCAAAGA
    CAGGAGAGCC ACCATGAGCA GTCAAGGGAC AGCTCCAGAC AGTCTGGAAC TGGACATGGA
    CATACCTCTG CTGGATCCGG AAATAGTAGG CACAGGGAAT CCAGTGTTAG TCAGGCCAGT
    GACAGTGAAG GACATTCAGA AGACTCAGGT AGACAGAACG TGACAACTCA TGGAAGGCCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCAGGGCC AGACAGGAGA CAGTTCTAGG
    TACTCAGCAT ATCATCAAGG GAGGCACAGT GAATCTGTCC ATGAAAGGTC AGCGTCTAGA
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACAGTCTGGA
    ACTGGACATG GACATGCCTC TGCTGGATCT GGAATCAACA GACATAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGAGA GGGACATGCA AGAGATTCAG GTAGACAGTC CGTGACAACT
    CATGGAAGGC CTGGTTCCAG CTCAAGAAAC CAACATGTAT CTTCCCATGG CCAGATAGGA
    GACAGTTCTA GCCACTCAGA GACCAATCAA GGGAGCCACA ATGAGAGACA TACAAGAGAT
    TCAGGTAGGC ATTCCGAGAC AACTCATGGA AGACTTGGTT CCAGCTCAAG GAACCAACAT
    GGATCTGTCC ATGGCCAGAC AAGAGACAGT CCTAGGCACT CAGAGTCCCA TCAAGAGAGG
    CACAGTGAAT CTGTCCATGA AAGGTCAGGA TCCAGCACTG GACAAAGACA GGGGAGCCAC
    CATGAGCAGT CAAGAGACAG CTCCAGACAC TCTGGAACTC GACATGGACA AACCTCTACT
    GTATCTGGAG GCAGTAGGCA CAGGGAACCC AGCGTTAGTC AGGCCAGTGA CAGTGAGGGA
    CATTCAGAAG ACTCAGGTAG ACAGTCCGTG ACAACTCATG GAAGGCCTGG TTCAACTTCA
    AGAAACCAAC ATGGATCTGT CCATGGCCAG ACAAGAGACA GTCCTAGGCA CTCAGAGTCC
    CATCAAGAGA GGCACAGTGA ATCTGTCCAC CAAAGGTCTG GATCAAGCAC AGGGCAAAGA
    CAGGGGAGCC ACCATGAGCA GTCAAGAGAC AGCTCCAGAC ACTCTGGAAC CGGTCATGGA
    CAAACCTCCG CTGGATCCGG AAGTAGTAGG CACAGGGAAT CCAGTGTTAG TCAGGCCAGT
    GACAGTGAAG GACATTCAGA AGACTCAGGT AGACAGAACG TGACAACTCA TGGAAGGCCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCAGGGCC AGACAGGAGA CAGTTCTAGG
    TACTCAGCAT CTCATCAAGG GAGGCACAGT GAATCTGTCC ATGAAAGGTC AACATCTAGA
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACAGTCTGGA
    ACTGGACATG GACATGCCTC TGCTGGATCT GGAATCAACA GACATAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GCAGACAGAA CGTGACAACT
    CATGGAAGGC CCGGTTCCAG CTCAAGCAAT CAACATGGAT CTTCCCATGG CCAGACTGGA
    GACAGTTCTA GGCACTCAGA GTCCCATCAA GGGAGCCACG GTGAATCTGT CCACCAAAGG
    TCTGGATCAA GCACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACACTCTG GAACTGGTCA TGGAGAAACA TCCACTGGAT CTGGAAGCAG CAGACATAGG
    GAATCCAGTG TTAGTCAGTC CAGTGACAGC AAGGGTCATT CAAGAGGTTC AGGTAATCAG
    ACTGTGACCA ACCAAAGAAT GTCTGCATTC TACTCAAGGT TCCAAAATCA TGGTGCAGGC
    CAAGTTTGGA GACATGGCAG CTATGGCAGT TCCAATTATG ACTATGGACA GTTAGGGACT
    GGACATTCTC CAGATGAAAA TTTTAGTCAC GATTCCAGTC ATGTGGAAAA GAGGGACAAA
    CCTGAATATA GAGGAGAACT AATGAGATCC AATATAACAG TCCGTAATAT ACACCCTGGA
    ACTTATGGCC ATTCAAATTA TATTTCAAAA CAGCTGGGAT TTGGACAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58346
    Clone ID Related Accession (Same CDS sequence) XM_010826841.2 , XM_010826840.1 , XM_010803069.2
    Accession Version XM_010826840.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product filaggrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644641.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCGACTC TCCTGGAAAA TATCAATGAC ATCATCAAAA TATTCCACAA ATATTCAAAA 
    ACAGATAAGG AGACTGACAC ATTGAGTGAA AAAGAGCTAA AGGAACTGGT GGAAGTGGAG
    TTTCGGCCCA TCCTGAAGAA TCCAGGTGAC CCGGACACCG CTGAAGTTTT CATGTATAAC
    CTCGATAGAG ATCACAACAA TAAAATAGAC TTTACAGAGT TTTTTCTGAT GGTATTCAAA
    GTGGCTCAAG TATATTATAG TTATACTCAA AGACAGAATT TACAGAGAGC AGGACAAAAA
    CAGAAAAAGT GTACATACCA CTACGGAGAT GAAGAAGATG ATACAGAAGA AGACAAAGAA
    GAAACAGAAA GAAAGTACAG TCATTCAAGA AGTGATGGAA AGACTCAAGA CAGATCAAAG
    AGCCCAAGAG GAAGAGGAAA AAAGAGACAT GGATCTAAAT CTGGAAGTAA ACAAAGAAGA
    GGTGACACAC CTACCTCTGG ACTCAGACAT GGATGTAGCA AAAAACATGA GTCAAGAAGG
    GAAAAGAAAA GGAGACCAAG CTCTACTGAA CCAAAAGAAA GAAGGCATAT GTCAAGTGTT
    AGCCCAACCA GGGGATATGA AGAAAAAGAA GAAGAACATG GTTATGAAAA TAAAGGTAAA
    ACAAGTGCAA AATGTATAGG ATCAGAGTAT GATGACTCCT ATCAAGTTTG TGAAGACAAA
    GTGACTACGA ATTTTCAACC CAGTCACAGT AAAAACTATG GGTCAAACAT CACGAAGGGC
    AGAGACACGG AAGGACATTC ACGAGATACA GGTAGGAAAT CCGTATTCAC TCATGCAAGA
    TCAGGGTCCA GTTCAAGGAA TCAAAATGGC TCTGTCCAGA CTCATACAGG AGACAATTCT
    ACCCACTCAG AATCCCAACA AGAGACAAAC AGTGAATCTG TCCACAGAAG GTCAAGAAAC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GACAACCCTC CACTGGATCC GGAGGCAGTA GACACAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GTAGACAGTC CGTGACAACT
    CATGGAAGGC CCGGTTCCAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGCACTCAGA GTCTCATCAA GGGAGGCACA GTGATTCTCT CCAAAGAAGG
    TCAGGTACTA GCACTGGGCA AAGACAGGGG AGCCACCACG AGCAGTCAAG AGACAGCTCC
    AGACACTCTG GAACTCAACA AGGACAAACC TCCACTGGAT CCGGAAGCAG TAGGCACAGA
    GACTCCAGTG TTAGTCAGGC CAGTGACAGT GAAGGACATT CAGAAGACTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ATCAACATGG ATCTTCCCAT
    GGCCAGACTG GAGACAGTTC TAGGCATTCA GAGTCCCATC AAAGAAGCCA CAGTGAAGCT
    GTTCATGAAA GGTCAGGATC CAGCACGGGG CAAAGGCAGA GGAGCCACCA TGAGCAGTCA
    AGGGACAGCT CCAGACACTC TGGAACTGGA CATGGACAAA TCTCAGCTGA ATCTGGAAAT
    GGCAGACATA GGGAATCCAG TGTTAGTCAA GCCAGTGACA GTGAGGGACA CGCCAGAGAT
    TCAGGTAGGC ATTCTGAGAC AACTCATGGA AGATATGGTT CCAGCTCAAG GAACCAACAT
    GGATCCTCCC ATGGTCAGAC AGGGGACAGT TATAGGCACT CAGAGACCAA TCAAGGGAGA
    CACAATAAGA GACACACAAG AGATTCAGGT AGGCATTCTG AGACAACTCA TGGAAGACCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCATGGCC AGAGTGGAGA CAGTTCTAGG
    CACTCAGAGT CCCATCAAGA GAGGCATAGT GAATCTGTCC ATGGAAGGTC AGGATCCAGC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGAG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GGCAAACCTC AGCTGAATCT GGAAATGGCA GACATAGGGA ATCCAGTGTT
    AGTCAAGCCA GTGACAGTGA GGGACACACC AGAGATTCAG GTAGGCATTC TGAGACAACT
    CATGGAAGGC CTGGTTCAAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGTACTCAGC ATATCATCAA GGGAGGCACA GTGAATCTGT CCATGAAAGG
    TCAGCGTCTA GAACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACAGTCTG GAACTGGACA TGGACATACC TCTGCTGGAT CTGGAAATGG CAGACATAGG
    GAATCCAGTG TTAGTCAGTC AAGTGACAGA GAGGGACACG CAAGAGATTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ACCAACATGT ATCTTCCCAT
    GGCCAGATAG GAGACAGTTC TAGCCACTCA GAGACCAATC AAGGGAGCCA CAATGAGAGA
    CATACAAGAG ATTCAGGTAG GCATTCCGAG ACAACTCATG GAAGACTTGG TTCCAGCTCA
    AGGAACCAAC ATGGATCTGT CCATGGCCAG ACAAGAGACA GTCCTAGGCA CTCAGAGTCC
    CATCAAGAGA GGCATAGTGA ATCTGTCCAT GGAAGGTCAG GATCCAGCAC TGGGCAAAGA
    CAGGAGAGCC ACCATGAGCA GTCAAGGGAC AGCTCCAGAC AGTCTGGAAC TGGACATGGA
    CATACCTCTG CTGGATCCGG AAATAGTAGG CACAGGGAAT CCAGTGTTAG TCAGGCCAGT
    GACAGTGAAG GACATTCAGA AGACTCAGGT AGACAGAACG TGACAACTCA TGGAAGGCCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCAGGGCC AGACAGGAGA CAGTTCTAGG
    TACTCAGCAT ATCATCAAGG GAGGCACAGT GAATCTGTCC ATGAAAGGTC AGCGTCTAGA
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACAGTCTGGA
    ACTGGACATG GACATGCCTC TGCTGGATCT GGAATCAACA GACATAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGAGA GGGACATGCA AGAGATTCAG GTAGACAGTC CGTGACAACT
    CATGGAAGGC CTGGTTCCAG CTCAAGAAAC CAACATGTAT CTTCCCATGG CCAGATAGGA
    GACAGTTCTA GCCACTCAGA GACCAATCAA GGGAGCCACA ATGAGAGACA TACAAGAGAT
    TCAGGTAGGC ATTCCGAGAC AACTCATGGA AGACTTGGTT CCAGCTCAAG GAACCAACAT
    GGATCTGTCC ATGGCCAGAC AAGAGACAGT CCTAGGCACT CAGAGTCCCA TCAAGAGAGG
    CACAGTGAAT CTGTCCATGA AAGGTCAGGA TCCAGCACTG GACAAAGACA GGGGAGCCAC
    CATGAGCAGT CAAGAGACAG CTCCAGACAC TCTGGAACTC GACATGGACA AACCTCTACT
    GTATCTGGAG GCAGTAGGCA CAGGGAACCC AGCGTTAGTC AGGCCAGTGA CAGTGAGGGA
    CATTCAGAAG ACTCAGGTAG ACAGTCCGTG ACAACTCATG GAAGGCCTGG TTCAACTTCA
    AGAAACCAAC ATGGATCTGT CCATGGCCAG ACAAGAGACA GTCCTAGGCA CTCAGAGTCC
    CATCAAGAGA GGCACAGTGA ATCTGTCCAC CAAAGGTCTG GATCAAGCAC AGGGCAAAGA
    CAGGGGAGCC ACCATGAGCA GTCAAGAGAC AGCTCCAGAC ACTCTGGAAC CGGTCATGGA
    CAAACCTCCG CTGGATCCGG AAGTAGTAGG CACAGGGAAT CCAGTGTTAG TCAGGCCAGT
    GACAGTGAAG GACATTCAGA AGACTCAGGT AGACAGAACG TGACAACTCA TGGAAGGCCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCAGGGCC AGACAGGAGA CAGTTCTAGG
    TACTCAGCAT CTCATCAAGG GAGGCACAGT GAATCTGTCC ATGAAAGGTC AACATCTAGA
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACAGTCTGGA
    ACTGGACATG GACATGCCTC TGCTGGATCT GGAATCAACA GACATAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GCAGACAGAA CGTGACAACT
    CATGGAAGGC CCGGTTCCAG CTCAAGCAAT CAACATGGAT CTTCCCATGG CCAGACTGGA
    GACAGTTCTA GGCACTCAGA GTCCCATCAA GGGAGCCACG GTGAATCTGT CCACCAAAGG
    TCTGGATCAA GCACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACACTCTG GAACTGGTCA TGGAGAAACA TCCACTGGAT CTGGAAGCAG CAGACATAGG
    GAATCCAGTG TTAGTCAGTC CAGTGACAGC AAGGGTCATT CAAGAGGTTC AGGTAATCAG
    ACTGTGACCA ACCAAAGAAT GTCTGCATTC TACTCAAGGT TCCAAAATCA TGGTGCAGGC
    CAAGTTTGGA GACATGGCAG CTATGGCAGT TCCAATTATG ACTATGGACA GTTAGGGACT
    GGACATTCTC CAGATGAAAA TTTTAGTCAC GATTCCAGTC ATGTGGAAAA GAGGGACAAA
    CCTGAATATA GAGGAGAACT AATGAGATCC AATATAACAG TCCGTAATAT ACACCCTGGA
    ACTTATGGCC ATTCAAATTA TATTTCAAAA CAGCTGGGAT TTGGACAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010825142.1
    CDS96..5066
    Translation

    Target ORF information:

    RefSeq Version XM_010826840.1
    Organism Bos taurus(cattle)
    Definition Bos taurus filaggrin (FLG), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826840.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCGACTC TCCTGGAAAA TATCAATGAC ATCATCAAAA TATTCCACAA ATATTCAAAA 
    ACAGATAAGG AGACTGACAC ATTGAGTGAA AAAGAGCTAA AGGAACTGGT GGAAGTGGAG
    TTTCGGCCCA TCCTGAAGAA TCCAGGTGAC CCGGACACCG CTGAAGTTTT CATGTATAAC
    CTCGATAGAG ATCACAACAA TAAAATAGAC TTTACAGAGT TTTTTCTGAT GGTATTCAAA
    GTGGCTCAAG TATATTATAG TTATACTCAA AGACAGAATT TACAGAGAGC AGGACAAAAA
    CAGAAAAAGT GTACATACCA CTACGGAGAT GAAGAAGATG ATACAGAAGA AGACAAAGAA
    GAAACAGAAA GAAAGTACAG TCATTCAAGA AGTGATGGAA AGACTCAAGA CAGATCAAAG
    AGCCCAAGAG GAAGAGGAAA AAAGAGACAT GGATCTAAAT CTGGAAGTAA ACAAAGAAGA
    GGTGACACAC CTACCTCTGG ACTCAGACAT GGATGTAGCA AAAAACATGA GTCAAGAAGG
    GAAAAGAAAA GGAGACCAAG CTCTACTGAA CCAAAAGAAA GAAGGCATAT GTCAAGTGTT
    AGCCCAACCA GGGGATATGA AGAAAAAGAA GAAGAACATG GTTATGAAAA TAAAGGTAAA
    ACAAGTGCAA AATGTATAGG ATCAGAGTAT GATGACTCCT ATCAAGTTTG TGAAGACAAA
    GTGACTACGA ATTTTCAACC CAGTCACAGT AAAAACTATG GGTCAAACAT CACGAAGGGC
    AGAGACACGG AAGGACATTC ACGAGATACA GGTAGGAAAT CCGTATTCAC TCATGCAAGA
    TCAGGGTCCA GTTCAAGGAA TCAAAATGGC TCTGTCCAGA CTCATACAGG AGACAATTCT
    ACCCACTCAG AATCCCAACA AGAGACAAAC AGTGAATCTG TCCACAGAAG GTCAAGAAAC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GACAACCCTC CACTGGATCC GGAGGCAGTA GACACAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GTAGACAGTC CGTGACAACT
    CATGGAAGGC CCGGTTCCAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGCACTCAGA GTCTCATCAA GGGAGGCACA GTGATTCTCT CCAAAGAAGG
    TCAGGTACTA GCACTGGGCA AAGACAGGGG AGCCACCACG AGCAGTCAAG AGACAGCTCC
    AGACACTCTG GAACTCAACA AGGACAAACC TCCACTGGAT CCGGAAGCAG TAGGCACAGA
    GACTCCAGTG TTAGTCAGGC CAGTGACAGT GAAGGACATT CAGAAGACTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ATCAACATGG ATCTTCCCAT
    GGCCAGACTG GAGACAGTTC TAGGCATTCA GAGTCCCATC AAAGAAGCCA CAGTGAAGCT
    GTTCATGAAA GGTCAGGATC CAGCACGGGG CAAAGGCAGA GGAGCCACCA TGAGCAGTCA
    AGGGACAGCT CCAGACACTC TGGAACTGGA CATGGACAAA TCTCAGCTGA ATCTGGAAAT
    GGCAGACATA GGGAATCCAG TGTTAGTCAA GCCAGTGACA GTGAGGGACA CGCCAGAGAT
    TCAGGTAGGC ATTCTGAGAC AACTCATGGA AGATATGGTT CCAGCTCAAG GAACCAACAT
    GGATCCTCCC ATGGTCAGAC AGGGGACAGT TATAGGCACT CAGAGACCAA TCAAGGGAGA
    CACAATAAGA GACACACAAG AGATTCAGGT AGGCATTCTG AGACAACTCA TGGAAGACCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCATGGCC AGAGTGGAGA CAGTTCTAGG
    CACTCAGAGT CCCATCAAGA GAGGCATAGT GAATCTGTCC ATGGAAGGTC AGGATCCAGC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGAG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GGCAAACCTC AGCTGAATCT GGAAATGGCA GACATAGGGA ATCCAGTGTT
    AGTCAAGCCA GTGACAGTGA GGGACACACC AGAGATTCAG GTAGGCATTC TGAGACAACT
    CATGGAAGGC CTGGTTCAAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGTACTCAGC ATATCATCAA GGGAGGCACA GTGAATCTGT CCATGAAAGG
    TCAGCGTCTA GAACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACAGTCTG GAACTGGACA TGGACATACC TCTGCTGGAT CTGGAAATGG CAGACATAGG
    GAATCCAGTG TTAGTCAGTC AAGTGACAGA GAGGGACACG CAAGAGATTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ACCAACATGT ATCTTCCCAT
    GGCCAGATAG GAGACAGTTC TAGCCACTCA GAGACCAATC AAGGGAGCCA CAATGAGAGA
    CATACAAGAG ATTCAGGTAG GCATTCCGAG ACAACTCATG GAAGACTTGG TTCCAGCTCA
    AGGAACCAAC ATGGATCTGT CCATGGCCAG ACAAGAGACA GTCCTAGGCA CTCAGAGTCC
    CATCAAGAGA GGCATAGTGA ATCTGTCCAT GGAAGGTCAG GATCCAGCAC TGGGCAAAGA
    CAGGAGAGCC ACCATGAGCA GTCAAGGGAC AGCTCCAGAC AGTCTGGAAC TGGACATGGA
    CATACCTCTG CTGGATCCGG AAATAGTAGG CACAGGGAAT CCAGTGTTAG TCAGGCCAGT
    GACAGTGAAG GACATTCAGA AGACTCAGGT AGACAGAACG TGACAACTCA TGGAAGGCCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCAGGGCC AGACAGGAGA CAGTTCTAGG
    TACTCAGCAT ATCATCAAGG GAGGCACAGT GAATCTGTCC ATGAAAGGTC AGCGTCTAGA
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACAGTCTGGA
    ACTGGACATG GACATGCCTC TGCTGGATCT GGAATCAACA GACATAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGAGA GGGACATGCA AGAGATTCAG GTAGACAGTC CGTGACAACT
    CATGGAAGGC CTGGTTCCAG CTCAAGAAAC CAACATGTAT CTTCCCATGG CCAGATAGGA
    GACAGTTCTA GCCACTCAGA GACCAATCAA GGGAGCCACA ATGAGAGACA TACAAGAGAT
    TCAGGTAGGC ATTCCGAGAC AACTCATGGA AGACTTGGTT CCAGCTCAAG GAACCAACAT
    GGATCTGTCC ATGGCCAGAC AAGAGACAGT CCTAGGCACT CAGAGTCCCA TCAAGAGAGG
    CACAGTGAAT CTGTCCATGA AAGGTCAGGA TCCAGCACTG GACAAAGACA GGGGAGCCAC
    CATGAGCAGT CAAGAGACAG CTCCAGACAC TCTGGAACTC GACATGGACA AACCTCTACT
    GTATCTGGAG GCAGTAGGCA CAGGGAACCC AGCGTTAGTC AGGCCAGTGA CAGTGAGGGA
    CATTCAGAAG ACTCAGGTAG ACAGTCCGTG ACAACTCATG GAAGGCCTGG TTCAACTTCA
    AGAAACCAAC ATGGATCTGT CCATGGCCAG ACAAGAGACA GTCCTAGGCA CTCAGAGTCC
    CATCAAGAGA GGCACAGTGA ATCTGTCCAC CAAAGGTCTG GATCAAGCAC AGGGCAAAGA
    CAGGGGAGCC ACCATGAGCA GTCAAGAGAC AGCTCCAGAC ACTCTGGAAC CGGTCATGGA
    CAAACCTCCG CTGGATCCGG AAGTAGTAGG CACAGGGAAT CCAGTGTTAG TCAGGCCAGT
    GACAGTGAAG GACATTCAGA AGACTCAGGT AGACAGAACG TGACAACTCA TGGAAGGCCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCAGGGCC AGACAGGAGA CAGTTCTAGG
    TACTCAGCAT CTCATCAAGG GAGGCACAGT GAATCTGTCC ATGAAAGGTC AACATCTAGA
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACAGTCTGGA
    ACTGGACATG GACATGCCTC TGCTGGATCT GGAATCAACA GACATAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GCAGACAGAA CGTGACAACT
    CATGGAAGGC CCGGTTCCAG CTCAAGCAAT CAACATGGAT CTTCCCATGG CCAGACTGGA
    GACAGTTCTA GGCACTCAGA GTCCCATCAA GGGAGCCACG GTGAATCTGT CCACCAAAGG
    TCTGGATCAA GCACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACACTCTG GAACTGGTCA TGGAGAAACA TCCACTGGAT CTGGAAGCAG CAGACATAGG
    GAATCCAGTG TTAGTCAGTC CAGTGACAGC AAGGGTCATT CAAGAGGTTC AGGTAATCAG
    ACTGTGACCA ACCAAAGAAT GTCTGCATTC TACTCAAGGT TCCAAAATCA TGGTGCAGGC
    CAAGTTTGGA GACATGGCAG CTATGGCAGT TCCAATTATG ACTATGGACA GTTAGGGACT
    GGACATTCTC CAGATGAAAA TTTTAGTCAC GATTCCAGTC ATGTGGAAAA GAGGGACAAA
    CCTGAATATA GAGGAGAACT AATGAGATCC AATATAACAG TCCGTAATAT ACACCCTGGA
    ACTTATGGCC ATTCAAATTA TATTTCAAAA CAGCTGGGAT TTGGACAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58346
    Clone ID Related Accession (Same CDS sequence) XM_010826841.2 , XM_010826840.1 , XM_010803069.2
    Accession Version XM_010803069.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product filaggrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103863.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803069.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCGACTC TCCTGGAAAA TATCAATGAC ATCATCAAAA TATTCCACAA ATATTCAAAA 
    ACAGATAAGG AGACTGACAC ATTGAGTGAA AAAGAGCTAA AGGAACTGGT GGAAGTGGAG
    TTTCGGCCCA TCCTGAAGAA TCCAGGTGAC CCGGACACCG CTGAAGTTTT CATGTATAAC
    CTCGATAGAG ATCACAACAA TAAAATAGAC TTTACAGAGT TTTTTCTGAT GGTATTCAAA
    GTGGCTCAAG TATATTATAG TTATACTCAA AGACAGAATT TACAGAGAGC AGGACAAAAA
    CAGAAAAAGT GTACATACCA CTACGGAGAT GAAGAAGATG ATACAGAAGA AGACAAAGAA
    GAAACAGAAA GAAAGTACAG TCATTCAAGA AGTGATGGAA AGACTCAAGA CAGATCAAAG
    AGCCCAAGAG GAAGAGGAAA AAAGAGACAT GGATCTAAAT CTGGAAGTAA ACAAAGAAGA
    GGTGACACAC CTACCTCTGG ACTCAGACAT GGATGTAGCA AAAAACATGA GTCAAGAAGG
    GAAAAGAAAA GGAGACCAAG CTCTACTGAA CCAAAAGAAA GAAGGCATAT GTCAAGTGTT
    AGCCCAACCA GGGGATATGA AGAAAAAGAA GAAGAACATG GTTATGAAAA TAAAGGTAAA
    ACAAGTGCAA AATGTATAGG ATCAGAGTAT GATGACTCCT ATCAAGTTTG TGAAGACAAA
    GTGACTACGA ATTTTCAACC CAGTCACAGT AAAAACTATG GGTCAAACAT CACGAAGGGC
    AGAGACACGG AAGGACATTC ACGAGATACA GGTAGGAAAT CCGTATTCAC TCATGCAAGA
    TCAGGGTCCA GTTCAAGGAA TCAAAATGGC TCTGTCCAGA CTCATACAGG AGACAATTCT
    ACCCACTCAG AATCCCAACA AGAGACAAAC AGTGAATCTG TCCACAGAAG GTCAAGAAAC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GACAACCCTC CACTGGATCC GGAGGCAGTA GACACAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GTAGACAGTC CGTGACAACT
    CATGGAAGGC CCGGTTCCAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGCACTCAGA GTCTCATCAA GGGAGGCACA GTGATTCTCT CCAAAGAAGG
    TCAGGTACTA GCACTGGGCA AAGACAGGGG AGCCACCACG AGCAGTCAAG AGACAGCTCC
    AGACACTCTG GAACTCAACA AGGACAAACC TCCACTGGAT CCGGAAGCAG TAGGCACAGA
    GACTCCAGTG TTAGTCAGGC CAGTGACAGT GAAGGACATT CAGAAGACTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ATCAACATGG ATCTTCCCAT
    GGCCAGACTG GAGACAGTTC TAGGCATTCA GAGTCCCATC AAAGAAGCCA CAGTGAAGCT
    GTTCATGAAA GGTCAGGATC CAGCACGGGG CAAAGGCAGA GGAGCCACCA TGAGCAGTCA
    AGGGACAGCT CCAGACACTC TGGAACTGGA CATGGACAAA TCTCAGCTGA ATCTGGAAAT
    GGCAGACATA GGGAATCCAG TGTTAGTCAA GCCAGTGACA GTGAGGGACA CGCCAGAGAT
    TCAGGTAGGC ATTCTGAGAC AACTCATGGA AGATATGGTT CCAGCTCAAG GAACCAACAT
    GGATCCTCCC ATGGTCAGAC AGGGGACAGT TATAGGCACT CAGAGACCAA TCAAGGGAGA
    CACAATAAGA GACACACAAG AGATTCAGGT AGGCATTCTG AGACAACTCA TGGAAGACCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCATGGCC AGAGTGGAGA CAGTTCTAGG
    CACTCAGAGT CCCATCAAGA GAGGCATAGT GAATCTGTCC ATGGAAGGTC AGGATCCAGC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGAG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GGCAAACCTC AGCTGAATCT GGAAATGGCA GACATAGGGA ATCCAGTGTT
    AGTCAAGCCA GTGACAGTGA GGGACACACC AGAGATTCAG GTAGGCATTC TGAGACAACT
    CATGGAAGGC CTGGTTCAAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGTACTCAGC ATATCATCAA GGGAGGCACA GTGAATCTGT CCATGAAAGG
    TCAGCGTCTA GAACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACAGTCTG GAACTGGACA TGGACATACC TCTGCTGGAT CTGGAAATGG CAGACATAGG
    GAATCCAGTG TTAGTCAGTC AAGTGACAGA GAGGGACACG CAAGAGATTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ACCAACATGT ATCTTCCCAT
    GGCCAGATAG GAGACAGTTC TAGCCACTCA GAGACCAATC AAGGGAGCCA CAATGAGAGA
    CATACAAGAG ATTCAGGTAG GCATTCCGAG ACAACTCATG GAAGACTTGG TTCCAGCTCA
    AGGAACCAAC ATGGATCTGT CCATGGCCAG ACAAGAGACA GTCCTAGGCA CTCAGAGTCC
    CATCAAGAGA GGCATAGTGA ATCTGTCCAT GGAAGGTCAG GATCCAGCAC TGGGCAAAGA
    CAGGAGAGCC ACCATGAGCA GTCAAGGGAC AGCTCCAGAC AGTCTGGAAC TGGACATGGA
    CATACCTCTG CTGGATCCGG AAATAGTAGG CACAGGGAAT CCAGTGTTAG TCAGGCCAGT
    GACAGTGAAG GACATTCAGA AGACTCAGGT AGACAGAACG TGACAACTCA TGGAAGGCCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCAGGGCC AGACAGGAGA CAGTTCTAGG
    TACTCAGCAT ATCATCAAGG GAGGCACAGT GAATCTGTCC ATGAAAGGTC AGCGTCTAGA
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACAGTCTGGA
    ACTGGACATG GACATGCCTC TGCTGGATCT GGAATCAACA GACATAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGAGA GGGACATGCA AGAGATTCAG GTAGACAGTC CGTGACAACT
    CATGGAAGGC CTGGTTCCAG CTCAAGAAAC CAACATGTAT CTTCCCATGG CCAGATAGGA
    GACAGTTCTA GCCACTCAGA GACCAATCAA GGGAGCCACA ATGAGAGACA TACAAGAGAT
    TCAGGTAGGC ATTCCGAGAC AACTCATGGA AGACTTGGTT CCAGCTCAAG GAACCAACAT
    GGATCTGTCC ATGGCCAGAC AAGAGACAGT CCTAGGCACT CAGAGTCCCA TCAAGAGAGG
    CACAGTGAAT CTGTCCATGA AAGGTCAGGA TCCAGCACTG GACAAAGACA GGGGAGCCAC
    CATGAGCAGT CAAGAGACAG CTCCAGACAC TCTGGAACTC GACATGGACA AACCTCTACT
    GTATCTGGAG GCAGTAGGCA CAGGGAACCC AGCGTTAGTC AGGCCAGTGA CAGTGAGGGA
    CATTCAGAAG ACTCAGGTAG ACAGTCCGTG ACAACTCATG GAAGGCCTGG TTCAACTTCA
    AGAAACCAAC ATGGATCTGT CCATGGCCAG ACAAGAGACA GTCCTAGGCA CTCAGAGTCC
    CATCAAGAGA GGCACAGTGA ATCTGTCCAC CAAAGGTCTG GATCAAGCAC AGGGCAAAGA
    CAGGGGAGCC ACCATGAGCA GTCAAGAGAC AGCTCCAGAC ACTCTGGAAC CGGTCATGGA
    CAAACCTCCG CTGGATCCGG AAGTAGTAGG CACAGGGAAT CCAGTGTTAG TCAGGCCAGT
    GACAGTGAAG GACATTCAGA AGACTCAGGT AGACAGAACG TGACAACTCA TGGAAGGCCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCAGGGCC AGACAGGAGA CAGTTCTAGG
    TACTCAGCAT CTCATCAAGG GAGGCACAGT GAATCTGTCC ATGAAAGGTC AACATCTAGA
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACAGTCTGGA
    ACTGGACATG GACATGCCTC TGCTGGATCT GGAATCAACA GACATAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GCAGACAGAA CGTGACAACT
    CATGGAAGGC CCGGTTCCAG CTCAAGCAAT CAACATGGAT CTTCCCATGG CCAGACTGGA
    GACAGTTCTA GGCACTCAGA GTCCCATCAA GGGAGCCACG GTGAATCTGT CCACCAAAGG
    TCTGGATCAA GCACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACACTCTG GAACTGGTCA TGGAGAAACA TCCACTGGAT CTGGAAGCAG CAGACATAGG
    GAATCCAGTG TTAGTCAGTC CAGTGACAGC AAGGGTCATT CAAGAGGTTC AGGTAATCAG
    ACTGTGACCA ACCAAAGAAT GTCTGCATTC TACTCAAGGT TCCAAAATCA TGGTGCAGGC
    CAAGTTTGGA GACATGGCAG CTATGGCAGT TCCAATTATG ACTATGGACA GTTAGGGACT
    GGACATTCTC CAGATGAAAA TTTTAGTCAC GATTCCAGTC ATGTGGAAAA GAGGGACAAA
    CCTGAATATA GAGGAGAACT AATGAGATCC AATATAACAG TCCGTAATAT ACACCCTGGA
    ACTTATGGCC ATTCAAATTA TATTTCAAAA CAGCTGGGAT TTGGACAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010801371.1
    CDS38..5008
    Translation

    Target ORF information:

    RefSeq Version XM_010803069.2
    Organism Bos taurus(cattle)
    Definition Bos taurus filaggrin (FLG), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010803069.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTCGACTC TCCTGGAAAA TATCAATGAC ATCATCAAAA TATTCCACAA ATATTCAAAA 
    ACAGATAAGG AGACTGACAC ATTGAGTGAA AAAGAGCTAA AGGAACTGGT GGAAGTGGAG
    TTTCGGCCCA TCCTGAAGAA TCCAGGTGAC CCGGACACCG CTGAAGTTTT CATGTATAAC
    CTCGATAGAG ATCACAACAA TAAAATAGAC TTTACAGAGT TTTTTCTGAT GGTATTCAAA
    GTGGCTCAAG TATATTATAG TTATACTCAA AGACAGAATT TACAGAGAGC AGGACAAAAA
    CAGAAAAAGT GTACATACCA CTACGGAGAT GAAGAAGATG ATACAGAAGA AGACAAAGAA
    GAAACAGAAA GAAAGTACAG TCATTCAAGA AGTGATGGAA AGACTCAAGA CAGATCAAAG
    AGCCCAAGAG GAAGAGGAAA AAAGAGACAT GGATCTAAAT CTGGAAGTAA ACAAAGAAGA
    GGTGACACAC CTACCTCTGG ACTCAGACAT GGATGTAGCA AAAAACATGA GTCAAGAAGG
    GAAAAGAAAA GGAGACCAAG CTCTACTGAA CCAAAAGAAA GAAGGCATAT GTCAAGTGTT
    AGCCCAACCA GGGGATATGA AGAAAAAGAA GAAGAACATG GTTATGAAAA TAAAGGTAAA
    ACAAGTGCAA AATGTATAGG ATCAGAGTAT GATGACTCCT ATCAAGTTTG TGAAGACAAA
    GTGACTACGA ATTTTCAACC CAGTCACAGT AAAAACTATG GGTCAAACAT CACGAAGGGC
    AGAGACACGG AAGGACATTC ACGAGATACA GGTAGGAAAT CCGTATTCAC TCATGCAAGA
    TCAGGGTCCA GTTCAAGGAA TCAAAATGGC TCTGTCCAGA CTCATACAGG AGACAATTCT
    ACCCACTCAG AATCCCAACA AGAGACAAAC AGTGAATCTG TCCACAGAAG GTCAAGAAAC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GACAACCCTC CACTGGATCC GGAGGCAGTA GACACAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GTAGACAGTC CGTGACAACT
    CATGGAAGGC CCGGTTCCAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGCACTCAGA GTCTCATCAA GGGAGGCACA GTGATTCTCT CCAAAGAAGG
    TCAGGTACTA GCACTGGGCA AAGACAGGGG AGCCACCACG AGCAGTCAAG AGACAGCTCC
    AGACACTCTG GAACTCAACA AGGACAAACC TCCACTGGAT CCGGAAGCAG TAGGCACAGA
    GACTCCAGTG TTAGTCAGGC CAGTGACAGT GAAGGACATT CAGAAGACTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ATCAACATGG ATCTTCCCAT
    GGCCAGACTG GAGACAGTTC TAGGCATTCA GAGTCCCATC AAAGAAGCCA CAGTGAAGCT
    GTTCATGAAA GGTCAGGATC CAGCACGGGG CAAAGGCAGA GGAGCCACCA TGAGCAGTCA
    AGGGACAGCT CCAGACACTC TGGAACTGGA CATGGACAAA TCTCAGCTGA ATCTGGAAAT
    GGCAGACATA GGGAATCCAG TGTTAGTCAA GCCAGTGACA GTGAGGGACA CGCCAGAGAT
    TCAGGTAGGC ATTCTGAGAC AACTCATGGA AGATATGGTT CCAGCTCAAG GAACCAACAT
    GGATCCTCCC ATGGTCAGAC AGGGGACAGT TATAGGCACT CAGAGACCAA TCAAGGGAGA
    CACAATAAGA GACACACAAG AGATTCAGGT AGGCATTCTG AGACAACTCA TGGAAGACCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCATGGCC AGAGTGGAGA CAGTTCTAGG
    CACTCAGAGT CCCATCAAGA GAGGCATAGT GAATCTGTCC ATGGAAGGTC AGGATCCAGC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGAG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GGCAAACCTC AGCTGAATCT GGAAATGGCA GACATAGGGA ATCCAGTGTT
    AGTCAAGCCA GTGACAGTGA GGGACACACC AGAGATTCAG GTAGGCATTC TGAGACAACT
    CATGGAAGGC CTGGTTCAAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGTACTCAGC ATATCATCAA GGGAGGCACA GTGAATCTGT CCATGAAAGG
    TCAGCGTCTA GAACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACAGTCTG GAACTGGACA TGGACATACC TCTGCTGGAT CTGGAAATGG CAGACATAGG
    GAATCCAGTG TTAGTCAGTC AAGTGACAGA GAGGGACACG CAAGAGATTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ACCAACATGT ATCTTCCCAT
    GGCCAGATAG GAGACAGTTC TAGCCACTCA GAGACCAATC AAGGGAGCCA CAATGAGAGA
    CATACAAGAG ATTCAGGTAG GCATTCCGAG ACAACTCATG GAAGACTTGG TTCCAGCTCA
    AGGAACCAAC ATGGATCTGT CCATGGCCAG ACAAGAGACA GTCCTAGGCA CTCAGAGTCC
    CATCAAGAGA GGCATAGTGA ATCTGTCCAT GGAAGGTCAG GATCCAGCAC TGGGCAAAGA
    CAGGAGAGCC ACCATGAGCA GTCAAGGGAC AGCTCCAGAC AGTCTGGAAC TGGACATGGA
    CATACCTCTG CTGGATCCGG AAATAGTAGG CACAGGGAAT CCAGTGTTAG TCAGGCCAGT
    GACAGTGAAG GACATTCAGA AGACTCAGGT AGACAGAACG TGACAACTCA TGGAAGGCCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCAGGGCC AGACAGGAGA CAGTTCTAGG
    TACTCAGCAT ATCATCAAGG GAGGCACAGT GAATCTGTCC ATGAAAGGTC AGCGTCTAGA
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACAGTCTGGA
    ACTGGACATG GACATGCCTC TGCTGGATCT GGAATCAACA GACATAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGAGA GGGACATGCA AGAGATTCAG GTAGACAGTC CGTGACAACT
    CATGGAAGGC CTGGTTCCAG CTCAAGAAAC CAACATGTAT CTTCCCATGG CCAGATAGGA
    GACAGTTCTA GCCACTCAGA GACCAATCAA GGGAGCCACA ATGAGAGACA TACAAGAGAT
    TCAGGTAGGC ATTCCGAGAC AACTCATGGA AGACTTGGTT CCAGCTCAAG GAACCAACAT
    GGATCTGTCC ATGGCCAGAC AAGAGACAGT CCTAGGCACT CAGAGTCCCA TCAAGAGAGG
    CACAGTGAAT CTGTCCATGA AAGGTCAGGA TCCAGCACTG GACAAAGACA GGGGAGCCAC
    CATGAGCAGT CAAGAGACAG CTCCAGACAC TCTGGAACTC GACATGGACA AACCTCTACT
    GTATCTGGAG GCAGTAGGCA CAGGGAACCC AGCGTTAGTC AGGCCAGTGA CAGTGAGGGA
    CATTCAGAAG ACTCAGGTAG ACAGTCCGTG ACAACTCATG GAAGGCCTGG TTCAACTTCA
    AGAAACCAAC ATGGATCTGT CCATGGCCAG ACAAGAGACA GTCCTAGGCA CTCAGAGTCC
    CATCAAGAGA GGCACAGTGA ATCTGTCCAC CAAAGGTCTG GATCAAGCAC AGGGCAAAGA
    CAGGGGAGCC ACCATGAGCA GTCAAGAGAC AGCTCCAGAC ACTCTGGAAC CGGTCATGGA
    CAAACCTCCG CTGGATCCGG AAGTAGTAGG CACAGGGAAT CCAGTGTTAG TCAGGCCAGT
    GACAGTGAAG GACATTCAGA AGACTCAGGT AGACAGAACG TGACAACTCA TGGAAGGCCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCAGGGCC AGACAGGAGA CAGTTCTAGG
    TACTCAGCAT CTCATCAAGG GAGGCACAGT GAATCTGTCC ATGAAAGGTC AACATCTAGA
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACAGTCTGGA
    ACTGGACATG GACATGCCTC TGCTGGATCT GGAATCAACA GACATAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GCAGACAGAA CGTGACAACT
    CATGGAAGGC CCGGTTCCAG CTCAAGCAAT CAACATGGAT CTTCCCATGG CCAGACTGGA
    GACAGTTCTA GGCACTCAGA GTCCCATCAA GGGAGCCACG GTGAATCTGT CCACCAAAGG
    TCTGGATCAA GCACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACACTCTG GAACTGGTCA TGGAGAAACA TCCACTGGAT CTGGAAGCAG CAGACATAGG
    GAATCCAGTG TTAGTCAGTC CAGTGACAGC AAGGGTCATT CAAGAGGTTC AGGTAATCAG
    ACTGTGACCA ACCAAAGAAT GTCTGCATTC TACTCAAGGT TCCAAAATCA TGGTGCAGGC
    CAAGTTTGGA GACATGGCAG CTATGGCAGT TCCAATTATG ACTATGGACA GTTAGGGACT
    GGACATTCTC CAGATGAAAA TTTTAGTCAC GATTCCAGTC ATGTGGAAAA GAGGGACAAA
    CCTGAATATA GAGGAGAACT AATGAGATCC AATATAACAG TCCGTAATAT ACACCCTGGA
    ACTTATGGCC ATTCAAATTA TATTTCAAAA CAGCTGGGAT TTGGACAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58348
    Clone ID Related Accession (Same CDS sequence) XM_010826843.1 , XM_010803071.2
    Accession Version XM_010826843.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product filaggrin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644641.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCGACTC TCCTGGAAAA TATCAATGAC ATCATCAAAA TATTCCACAA ATATTCAAAA 
    ACAGATAAGG AGACTGACAC ATTGAGTGAA AAAGAGCTAA AGGAACTGGT GGAAGTGGAG
    TTTCGGCCCA TCCTGAAGAA TCCAGGTGAC CCGGACACCG CTGAAGTTTT CATGTATAAC
    CTCGATAGAG ATCACAACAA TAAAATAGAC TTTACAGAGT TTTTTCTGAT GGTATTCAAA
    GTGGCTCAAG TATATTATAG TTATACTCAA AGACAGAATT TACAGAGAGC AGGACAAAAA
    CAGAAAAAGT GTACATACCA CTACGGAGAT GAAGAAGATG ATACAGAAGA AGACAAAGAA
    GAAACAGAAA GAAAGTACAG TCATTCAAGA AGTGATGGAA AGACTCAAGA CAGATCAAAG
    AGCCCAAGAG GAAGAGGAAA AAAGAGACAT GGATCTAAAT CTGGAAGTAA ACAAAGAAGA
    GGTGACACAC CTACCTCTGG ACTCAGACAT GGATGTAGCA AAAAACATGA GTCAAGAAGG
    GAAAAGAAAA GGAGACCAAG CTCTACTGAA CCAAAAGAAA GAAGGCATAT GTCAAGTGTT
    AGCCCAACCA GGGGATATGA AGAAAAAGAA GAAGAACATG GTTATGAAAA TAAAGGTAAA
    ACAAGTGCAA AATGTATAGG ATCAGAGTAT GATGACTCCT ATCAAGTTTG TGAAGACAAA
    GTGACTACGA ATTTTCAACC CAGTCACAGT AAAAACTATG GGTCAAACAT CACGAAGGGC
    AGAGACACGG AAGGACATTC ACGAGATACA GGTAGGAAAT CCGTATTCAC TCATGCAAGA
    TCAGGGTCCA GTTCAAGGAA TCAAAATGGC TCTGTCCAGA CTCATACAGG AGACAATTCT
    ACCCACTCAG AATCCCAACA AGAGACAAAC AGTGAATCTG TCCACAGAAG GTCAAGAAAC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GACAACCCTC CACTGGATCC GGAGGCAGTA GACACAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GTAGACAGTC CGTGACAACT
    CATGGAAGGC CCGGTTCCAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGCACTCAGA GTCTCATCAA GGGAGGCACA GTGATTCTCT CCAAAGAAGG
    TCAGGTACTA GCACTGGGCA AAGACAGGGG AGCCACCACG AGCAGTCAAG AGACAGCTCC
    AGACACTCTG GAACTCAACA AGGACAAACC TCCACTGGAT CCGGAAGCAG TAGGCACAGA
    GACTCCAGTG TTAGTCAGGC CAGTGACAGT GAAGGACATT CAGAAGACTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ATCAACATGG ATCTTCCCAT
    GGCCAGACTG GAGACAGTTC TAGGCATTCA GAGTCCCATC AAAGAAGCCA CAGTGAAGCT
    GTTCATGAAA GGTCAGGATC CAGCACGGGG CAAAGGCAGA GGAGCCACCA TGAGCAGTCA
    AGGGACAGCT CCAGACACTC TGGAACTGGA CATGGACAAA TCTCAGCTGA ATCTGGAAAT
    GGCAGACATA GGGAATCCAG TGTTAGTCAA GCCAGTGACA GTGAGGGACA CGCCAGAGAT
    TCAGGTAGGC ATTCTGAGAC AACTCATGGA AGATATGGTT CCAGCTCAAG GAACCAACAT
    GGATCCTCCC ATGGTCAGAC AGGGGACAGT TATAGGCACT CAGAGACCAA TCAAGGGAGA
    CACAATAAGA GACACACAAG AGATTCAGGT AGGCATTCTG AGACAACTCA TGGAAGACCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCATGGCC AGAGTGGAGA CAGTTCTAGG
    CACTCAGAGT CCCATCAAGA GAGGCATAGT GAATCTGTCC ATGGAAGGTC AGGATCCAGC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGAG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GGCAAACCTC AGCTGAATCT GGAAATGGCA GACATAGGGA ATCCAGTGTT
    AGTCAAGCCA GTGACAGTGA GGGACACACC AGAGATTCAG GTAGGCATTC TGAGACAACT
    CATGGAAGGC CTGGTTCAAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGTACTCAGC ATATCATCAA GGGAGGCACA GTGAATCTGT CCATGAAAGG
    TCAGCGTCTA GAACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACAGTCTG GAACTGGACA TGGACATGCC TCTGCTGGAT CTGGAATCAA CAGACATAGG
    GAATCCAGTG TTAGTCAGGC CAGTGACAGA GAGGGACATG CAAGAGATTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ACCAACATGT ATCTTCCCAT
    GGCCAGATAG GAGACAGTTC TAGCCACTCA GAGACCAATC AAGGGAGCCA CAATGAGAGA
    CATACAAGAG ATTCAGGTAG GCATTCCGAG ACAACTCATG GAAGACTTGG TTCCAGCTCA
    AGGAACCAAC ATGGATCTGT CCATGGCCAG ACAAGAGACA GTCCTAGGCA CTCAGAGTCC
    CATCAAGAGA GGCACAGTGA ATCTGTCCAT GAAAGGTCAG GATCCAGCAC TGGACAAAGA
    CAGGGGAGCC ACCATGAGCA GTCAAGAGAC AGCTCCAGAC ACTCTGGAAC TCGACATGGA
    CAAACCTCTA CTGTATCTGG AGGCAGTAGG CACAGGGAAC CCAGCGTTAG TCAGGCCAGT
    GACAGTGAGG GACATTCAGA AGACTCAGGT AGACAGTCCG TGACAACTCA TGGAAGGCCT
    GGTTCAACTT CAAGAAACCA ACATGGATCT GTCCATGGCC AGACAAGAGA CAGTCCTAGG
    CACTCAGAGT CCCATCAAGA GAGGCACAGT GAATCTGTCC ACCAAAGGTC TGGATCAAGC
    ACAGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGAG ACAGCTCCAG ACACTCTGGA
    ACCGGTCATG GACAAACCTC CGCTGGATCC GGAAGTAGTA GGCACAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GTAGACAGAA CGTGACAACT
    CATGGAAGGC CTGGTTCCAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGTACTCAGC ATCTCATCAA GGGAGGCACA GTGAATCTGT CCATGAAAGG
    TCAACATCTA GAACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACAGTCTG GAACTGGACA TGGACATGCC TCTGCTGGAT CTGGAATCAA CAGACATAGG
    GAATCCAGTG TTAGTCAGGC CAGTGACAGT GAAGGACATT CAGAAGACTC AGGCAGACAG
    AACGTGACAA CTCATGGAAG GCCCGGTTCC AGCTCAAGCA ATCAACATGG ATCTTCCCAT
    GGCCAGACTG GAGACAGTTC TAGGCACTCA GAGTCCCATC AAGGGAGCCA CGGTGAATCT
    GTCCACCAAA GGTCTGGATC AAGCACTGGG CAAAGACAGG GGAGCCACCA TGAGCAGTCA
    AGGGACAGCT CCAGACACTC TGGAACTGGT CATGGAGAAA CATCCACTGG ATCTGGAAGC
    AGCAGACATA GGGAATCCAG TGTTAGTCAG TCCAGTGACA GCAAGGGTCA TTCAAGAGGT
    TCAGGTAATC AGACTGTGAC CAACCAAAGA ATGTCTGCAT TCTACTCAAG GTTCCAAAAT
    CATGGTGCAG GCCAAGTTTG GAGACATGGC AGCTATGGCA GTTCCAATTA TGACTATGGA
    CAGTTAGGGA CTGGACATTC TCCAGATGAA AATTTTAGTC ACGATTCCAG TCATGTGGAA
    AAGAGGGACA AACCTGAATA TAGAGGAGAA CTAATGAGAT CCAATATAAC AGTCCGTAAT
    ATACACCCTG GAACTTATGG CCATTCAAAT TATATTTCAA AACAGCTGGG ATTTGGACAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010825145.1
    CDS96..4298
    Translation

    Target ORF information:

    RefSeq Version XM_010826843.1
    Organism Bos taurus(cattle)
    Definition Bos taurus filaggrin (FLG), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826843.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCGACTC TCCTGGAAAA TATCAATGAC ATCATCAAAA TATTCCACAA ATATTCAAAA 
    ACAGATAAGG AGACTGACAC ATTGAGTGAA AAAGAGCTAA AGGAACTGGT GGAAGTGGAG
    TTTCGGCCCA TCCTGAAGAA TCCAGGTGAC CCGGACACCG CTGAAGTTTT CATGTATAAC
    CTCGATAGAG ATCACAACAA TAAAATAGAC TTTACAGAGT TTTTTCTGAT GGTATTCAAA
    GTGGCTCAAG TATATTATAG TTATACTCAA AGACAGAATT TACAGAGAGC AGGACAAAAA
    CAGAAAAAGT GTACATACCA CTACGGAGAT GAAGAAGATG ATACAGAAGA AGACAAAGAA
    GAAACAGAAA GAAAGTACAG TCATTCAAGA AGTGATGGAA AGACTCAAGA CAGATCAAAG
    AGCCCAAGAG GAAGAGGAAA AAAGAGACAT GGATCTAAAT CTGGAAGTAA ACAAAGAAGA
    GGTGACACAC CTACCTCTGG ACTCAGACAT GGATGTAGCA AAAAACATGA GTCAAGAAGG
    GAAAAGAAAA GGAGACCAAG CTCTACTGAA CCAAAAGAAA GAAGGCATAT GTCAAGTGTT
    AGCCCAACCA GGGGATATGA AGAAAAAGAA GAAGAACATG GTTATGAAAA TAAAGGTAAA
    ACAAGTGCAA AATGTATAGG ATCAGAGTAT GATGACTCCT ATCAAGTTTG TGAAGACAAA
    GTGACTACGA ATTTTCAACC CAGTCACAGT AAAAACTATG GGTCAAACAT CACGAAGGGC
    AGAGACACGG AAGGACATTC ACGAGATACA GGTAGGAAAT CCGTATTCAC TCATGCAAGA
    TCAGGGTCCA GTTCAAGGAA TCAAAATGGC TCTGTCCAGA CTCATACAGG AGACAATTCT
    ACCCACTCAG AATCCCAACA AGAGACAAAC AGTGAATCTG TCCACAGAAG GTCAAGAAAC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GACAACCCTC CACTGGATCC GGAGGCAGTA GACACAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GTAGACAGTC CGTGACAACT
    CATGGAAGGC CCGGTTCCAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGCACTCAGA GTCTCATCAA GGGAGGCACA GTGATTCTCT CCAAAGAAGG
    TCAGGTACTA GCACTGGGCA AAGACAGGGG AGCCACCACG AGCAGTCAAG AGACAGCTCC
    AGACACTCTG GAACTCAACA AGGACAAACC TCCACTGGAT CCGGAAGCAG TAGGCACAGA
    GACTCCAGTG TTAGTCAGGC CAGTGACAGT GAAGGACATT CAGAAGACTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ATCAACATGG ATCTTCCCAT
    GGCCAGACTG GAGACAGTTC TAGGCATTCA GAGTCCCATC AAAGAAGCCA CAGTGAAGCT
    GTTCATGAAA GGTCAGGATC CAGCACGGGG CAAAGGCAGA GGAGCCACCA TGAGCAGTCA
    AGGGACAGCT CCAGACACTC TGGAACTGGA CATGGACAAA TCTCAGCTGA ATCTGGAAAT
    GGCAGACATA GGGAATCCAG TGTTAGTCAA GCCAGTGACA GTGAGGGACA CGCCAGAGAT
    TCAGGTAGGC ATTCTGAGAC AACTCATGGA AGATATGGTT CCAGCTCAAG GAACCAACAT
    GGATCCTCCC ATGGTCAGAC AGGGGACAGT TATAGGCACT CAGAGACCAA TCAAGGGAGA
    CACAATAAGA GACACACAAG AGATTCAGGT AGGCATTCTG AGACAACTCA TGGAAGACCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCATGGCC AGAGTGGAGA CAGTTCTAGG
    CACTCAGAGT CCCATCAAGA GAGGCATAGT GAATCTGTCC ATGGAAGGTC AGGATCCAGC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGAG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GGCAAACCTC AGCTGAATCT GGAAATGGCA GACATAGGGA ATCCAGTGTT
    AGTCAAGCCA GTGACAGTGA GGGACACACC AGAGATTCAG GTAGGCATTC TGAGACAACT
    CATGGAAGGC CTGGTTCAAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGTACTCAGC ATATCATCAA GGGAGGCACA GTGAATCTGT CCATGAAAGG
    TCAGCGTCTA GAACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACAGTCTG GAACTGGACA TGGACATGCC TCTGCTGGAT CTGGAATCAA CAGACATAGG
    GAATCCAGTG TTAGTCAGGC CAGTGACAGA GAGGGACATG CAAGAGATTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ACCAACATGT ATCTTCCCAT
    GGCCAGATAG GAGACAGTTC TAGCCACTCA GAGACCAATC AAGGGAGCCA CAATGAGAGA
    CATACAAGAG ATTCAGGTAG GCATTCCGAG ACAACTCATG GAAGACTTGG TTCCAGCTCA
    AGGAACCAAC ATGGATCTGT CCATGGCCAG ACAAGAGACA GTCCTAGGCA CTCAGAGTCC
    CATCAAGAGA GGCACAGTGA ATCTGTCCAT GAAAGGTCAG GATCCAGCAC TGGACAAAGA
    CAGGGGAGCC ACCATGAGCA GTCAAGAGAC AGCTCCAGAC ACTCTGGAAC TCGACATGGA
    CAAACCTCTA CTGTATCTGG AGGCAGTAGG CACAGGGAAC CCAGCGTTAG TCAGGCCAGT
    GACAGTGAGG GACATTCAGA AGACTCAGGT AGACAGTCCG TGACAACTCA TGGAAGGCCT
    GGTTCAACTT CAAGAAACCA ACATGGATCT GTCCATGGCC AGACAAGAGA CAGTCCTAGG
    CACTCAGAGT CCCATCAAGA GAGGCACAGT GAATCTGTCC ACCAAAGGTC TGGATCAAGC
    ACAGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGAG ACAGCTCCAG ACACTCTGGA
    ACCGGTCATG GACAAACCTC CGCTGGATCC GGAAGTAGTA GGCACAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GTAGACAGAA CGTGACAACT
    CATGGAAGGC CTGGTTCCAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGTACTCAGC ATCTCATCAA GGGAGGCACA GTGAATCTGT CCATGAAAGG
    TCAACATCTA GAACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACAGTCTG GAACTGGACA TGGACATGCC TCTGCTGGAT CTGGAATCAA CAGACATAGG
    GAATCCAGTG TTAGTCAGGC CAGTGACAGT GAAGGACATT CAGAAGACTC AGGCAGACAG
    AACGTGACAA CTCATGGAAG GCCCGGTTCC AGCTCAAGCA ATCAACATGG ATCTTCCCAT
    GGCCAGACTG GAGACAGTTC TAGGCACTCA GAGTCCCATC AAGGGAGCCA CGGTGAATCT
    GTCCACCAAA GGTCTGGATC AAGCACTGGG CAAAGACAGG GGAGCCACCA TGAGCAGTCA
    AGGGACAGCT CCAGACACTC TGGAACTGGT CATGGAGAAA CATCCACTGG ATCTGGAAGC
    AGCAGACATA GGGAATCCAG TGTTAGTCAG TCCAGTGACA GCAAGGGTCA TTCAAGAGGT
    TCAGGTAATC AGACTGTGAC CAACCAAAGA ATGTCTGCAT TCTACTCAAG GTTCCAAAAT
    CATGGTGCAG GCCAAGTTTG GAGACATGGC AGCTATGGCA GTTCCAATTA TGACTATGGA
    CAGTTAGGGA CTGGACATTC TCCAGATGAA AATTTTAGTC ACGATTCCAG TCATGTGGAA
    AAGAGGGACA AACCTGAATA TAGAGGAGAA CTAATGAGAT CCAATATAAC AGTCCGTAAT
    ATACACCCTG GAACTTATGG CCATTCAAAT TATATTTCAA AACAGCTGGG ATTTGGACAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58348
    Clone ID Related Accession (Same CDS sequence) XM_010826843.1 , XM_010803071.2
    Accession Version XM_010803071.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product filaggrin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103863.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803071.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCGACTC TCCTGGAAAA TATCAATGAC ATCATCAAAA TATTCCACAA ATATTCAAAA 
    ACAGATAAGG AGACTGACAC ATTGAGTGAA AAAGAGCTAA AGGAACTGGT GGAAGTGGAG
    TTTCGGCCCA TCCTGAAGAA TCCAGGTGAC CCGGACACCG CTGAAGTTTT CATGTATAAC
    CTCGATAGAG ATCACAACAA TAAAATAGAC TTTACAGAGT TTTTTCTGAT GGTATTCAAA
    GTGGCTCAAG TATATTATAG TTATACTCAA AGACAGAATT TACAGAGAGC AGGACAAAAA
    CAGAAAAAGT GTACATACCA CTACGGAGAT GAAGAAGATG ATACAGAAGA AGACAAAGAA
    GAAACAGAAA GAAAGTACAG TCATTCAAGA AGTGATGGAA AGACTCAAGA CAGATCAAAG
    AGCCCAAGAG GAAGAGGAAA AAAGAGACAT GGATCTAAAT CTGGAAGTAA ACAAAGAAGA
    GGTGACACAC CTACCTCTGG ACTCAGACAT GGATGTAGCA AAAAACATGA GTCAAGAAGG
    GAAAAGAAAA GGAGACCAAG CTCTACTGAA CCAAAAGAAA GAAGGCATAT GTCAAGTGTT
    AGCCCAACCA GGGGATATGA AGAAAAAGAA GAAGAACATG GTTATGAAAA TAAAGGTAAA
    ACAAGTGCAA AATGTATAGG ATCAGAGTAT GATGACTCCT ATCAAGTTTG TGAAGACAAA
    GTGACTACGA ATTTTCAACC CAGTCACAGT AAAAACTATG GGTCAAACAT CACGAAGGGC
    AGAGACACGG AAGGACATTC ACGAGATACA GGTAGGAAAT CCGTATTCAC TCATGCAAGA
    TCAGGGTCCA GTTCAAGGAA TCAAAATGGC TCTGTCCAGA CTCATACAGG AGACAATTCT
    ACCCACTCAG AATCCCAACA AGAGACAAAC AGTGAATCTG TCCACAGAAG GTCAAGAAAC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GACAACCCTC CACTGGATCC GGAGGCAGTA GACACAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GTAGACAGTC CGTGACAACT
    CATGGAAGGC CCGGTTCCAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGCACTCAGA GTCTCATCAA GGGAGGCACA GTGATTCTCT CCAAAGAAGG
    TCAGGTACTA GCACTGGGCA AAGACAGGGG AGCCACCACG AGCAGTCAAG AGACAGCTCC
    AGACACTCTG GAACTCAACA AGGACAAACC TCCACTGGAT CCGGAAGCAG TAGGCACAGA
    GACTCCAGTG TTAGTCAGGC CAGTGACAGT GAAGGACATT CAGAAGACTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ATCAACATGG ATCTTCCCAT
    GGCCAGACTG GAGACAGTTC TAGGCATTCA GAGTCCCATC AAAGAAGCCA CAGTGAAGCT
    GTTCATGAAA GGTCAGGATC CAGCACGGGG CAAAGGCAGA GGAGCCACCA TGAGCAGTCA
    AGGGACAGCT CCAGACACTC TGGAACTGGA CATGGACAAA TCTCAGCTGA ATCTGGAAAT
    GGCAGACATA GGGAATCCAG TGTTAGTCAA GCCAGTGACA GTGAGGGACA CGCCAGAGAT
    TCAGGTAGGC ATTCTGAGAC AACTCATGGA AGATATGGTT CCAGCTCAAG GAACCAACAT
    GGATCCTCCC ATGGTCAGAC AGGGGACAGT TATAGGCACT CAGAGACCAA TCAAGGGAGA
    CACAATAAGA GACACACAAG AGATTCAGGT AGGCATTCTG AGACAACTCA TGGAAGACCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCATGGCC AGAGTGGAGA CAGTTCTAGG
    CACTCAGAGT CCCATCAAGA GAGGCATAGT GAATCTGTCC ATGGAAGGTC AGGATCCAGC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGAG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GGCAAACCTC AGCTGAATCT GGAAATGGCA GACATAGGGA ATCCAGTGTT
    AGTCAAGCCA GTGACAGTGA GGGACACACC AGAGATTCAG GTAGGCATTC TGAGACAACT
    CATGGAAGGC CTGGTTCAAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGTACTCAGC ATATCATCAA GGGAGGCACA GTGAATCTGT CCATGAAAGG
    TCAGCGTCTA GAACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACAGTCTG GAACTGGACA TGGACATGCC TCTGCTGGAT CTGGAATCAA CAGACATAGG
    GAATCCAGTG TTAGTCAGGC CAGTGACAGA GAGGGACATG CAAGAGATTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ACCAACATGT ATCTTCCCAT
    GGCCAGATAG GAGACAGTTC TAGCCACTCA GAGACCAATC AAGGGAGCCA CAATGAGAGA
    CATACAAGAG ATTCAGGTAG GCATTCCGAG ACAACTCATG GAAGACTTGG TTCCAGCTCA
    AGGAACCAAC ATGGATCTGT CCATGGCCAG ACAAGAGACA GTCCTAGGCA CTCAGAGTCC
    CATCAAGAGA GGCACAGTGA ATCTGTCCAT GAAAGGTCAG GATCCAGCAC TGGACAAAGA
    CAGGGGAGCC ACCATGAGCA GTCAAGAGAC AGCTCCAGAC ACTCTGGAAC TCGACATGGA
    CAAACCTCTA CTGTATCTGG AGGCAGTAGG CACAGGGAAC CCAGCGTTAG TCAGGCCAGT
    GACAGTGAGG GACATTCAGA AGACTCAGGT AGACAGTCCG TGACAACTCA TGGAAGGCCT
    GGTTCAACTT CAAGAAACCA ACATGGATCT GTCCATGGCC AGACAAGAGA CAGTCCTAGG
    CACTCAGAGT CCCATCAAGA GAGGCACAGT GAATCTGTCC ACCAAAGGTC TGGATCAAGC
    ACAGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGAG ACAGCTCCAG ACACTCTGGA
    ACCGGTCATG GACAAACCTC CGCTGGATCC GGAAGTAGTA GGCACAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GTAGACAGAA CGTGACAACT
    CATGGAAGGC CTGGTTCCAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGTACTCAGC ATCTCATCAA GGGAGGCACA GTGAATCTGT CCATGAAAGG
    TCAACATCTA GAACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACAGTCTG GAACTGGACA TGGACATGCC TCTGCTGGAT CTGGAATCAA CAGACATAGG
    GAATCCAGTG TTAGTCAGGC CAGTGACAGT GAAGGACATT CAGAAGACTC AGGCAGACAG
    AACGTGACAA CTCATGGAAG GCCCGGTTCC AGCTCAAGCA ATCAACATGG ATCTTCCCAT
    GGCCAGACTG GAGACAGTTC TAGGCACTCA GAGTCCCATC AAGGGAGCCA CGGTGAATCT
    GTCCACCAAA GGTCTGGATC AAGCACTGGG CAAAGACAGG GGAGCCACCA TGAGCAGTCA
    AGGGACAGCT CCAGACACTC TGGAACTGGT CATGGAGAAA CATCCACTGG ATCTGGAAGC
    AGCAGACATA GGGAATCCAG TGTTAGTCAG TCCAGTGACA GCAAGGGTCA TTCAAGAGGT
    TCAGGTAATC AGACTGTGAC CAACCAAAGA ATGTCTGCAT TCTACTCAAG GTTCCAAAAT
    CATGGTGCAG GCCAAGTTTG GAGACATGGC AGCTATGGCA GTTCCAATTA TGACTATGGA
    CAGTTAGGGA CTGGACATTC TCCAGATGAA AATTTTAGTC ACGATTCCAG TCATGTGGAA
    AAGAGGGACA AACCTGAATA TAGAGGAGAA CTAATGAGAT CCAATATAAC AGTCCGTAAT
    ATACACCCTG GAACTTATGG CCATTCAAAT TATATTTCAA AACAGCTGGG ATTTGGACAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010801373.1
    CDS96..4298
    Translation

    Target ORF information:

    RefSeq Version XM_010803071.2
    Organism Bos taurus(cattle)
    Definition Bos taurus filaggrin (FLG), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010803071.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCGACTC TCCTGGAAAA TATCAATGAC ATCATCAAAA TATTCCACAA ATATTCAAAA 
    ACAGATAAGG AGACTGACAC ATTGAGTGAA AAAGAGCTAA AGGAACTGGT GGAAGTGGAG
    TTTCGGCCCA TCCTGAAGAA TCCAGGTGAC CCGGACACCG CTGAAGTTTT CATGTATAAC
    CTCGATAGAG ATCACAACAA TAAAATAGAC TTTACAGAGT TTTTTCTGAT GGTATTCAAA
    GTGGCTCAAG TATATTATAG TTATACTCAA AGACAGAATT TACAGAGAGC AGGACAAAAA
    CAGAAAAAGT GTACATACCA CTACGGAGAT GAAGAAGATG ATACAGAAGA AGACAAAGAA
    GAAACAGAAA GAAAGTACAG TCATTCAAGA AGTGATGGAA AGACTCAAGA CAGATCAAAG
    AGCCCAAGAG GAAGAGGAAA AAAGAGACAT GGATCTAAAT CTGGAAGTAA ACAAAGAAGA
    GGTGACACAC CTACCTCTGG ACTCAGACAT GGATGTAGCA AAAAACATGA GTCAAGAAGG
    GAAAAGAAAA GGAGACCAAG CTCTACTGAA CCAAAAGAAA GAAGGCATAT GTCAAGTGTT
    AGCCCAACCA GGGGATATGA AGAAAAAGAA GAAGAACATG GTTATGAAAA TAAAGGTAAA
    ACAAGTGCAA AATGTATAGG ATCAGAGTAT GATGACTCCT ATCAAGTTTG TGAAGACAAA
    GTGACTACGA ATTTTCAACC CAGTCACAGT AAAAACTATG GGTCAAACAT CACGAAGGGC
    AGAGACACGG AAGGACATTC ACGAGATACA GGTAGGAAAT CCGTATTCAC TCATGCAAGA
    TCAGGGTCCA GTTCAAGGAA TCAAAATGGC TCTGTCCAGA CTCATACAGG AGACAATTCT
    ACCCACTCAG AATCCCAACA AGAGACAAAC AGTGAATCTG TCCACAGAAG GTCAAGAAAC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGGG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GACAACCCTC CACTGGATCC GGAGGCAGTA GACACAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GTAGACAGTC CGTGACAACT
    CATGGAAGGC CCGGTTCCAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGCACTCAGA GTCTCATCAA GGGAGGCACA GTGATTCTCT CCAAAGAAGG
    TCAGGTACTA GCACTGGGCA AAGACAGGGG AGCCACCACG AGCAGTCAAG AGACAGCTCC
    AGACACTCTG GAACTCAACA AGGACAAACC TCCACTGGAT CCGGAAGCAG TAGGCACAGA
    GACTCCAGTG TTAGTCAGGC CAGTGACAGT GAAGGACATT CAGAAGACTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ATCAACATGG ATCTTCCCAT
    GGCCAGACTG GAGACAGTTC TAGGCATTCA GAGTCCCATC AAAGAAGCCA CAGTGAAGCT
    GTTCATGAAA GGTCAGGATC CAGCACGGGG CAAAGGCAGA GGAGCCACCA TGAGCAGTCA
    AGGGACAGCT CCAGACACTC TGGAACTGGA CATGGACAAA TCTCAGCTGA ATCTGGAAAT
    GGCAGACATA GGGAATCCAG TGTTAGTCAA GCCAGTGACA GTGAGGGACA CGCCAGAGAT
    TCAGGTAGGC ATTCTGAGAC AACTCATGGA AGATATGGTT CCAGCTCAAG GAACCAACAT
    GGATCCTCCC ATGGTCAGAC AGGGGACAGT TATAGGCACT CAGAGACCAA TCAAGGGAGA
    CACAATAAGA GACACACAAG AGATTCAGGT AGGCATTCTG AGACAACTCA TGGAAGACCT
    GGTTCCAGCT CAAGAAACCA ACATGGATCT TCCCATGGCC AGAGTGGAGA CAGTTCTAGG
    CACTCAGAGT CCCATCAAGA GAGGCATAGT GAATCTGTCC ATGGAAGGTC AGGATCCAGC
    ACTGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGAG ACAGCTCCAG ACACTCTGGA
    ACTCGACATG GGCAAACCTC AGCTGAATCT GGAAATGGCA GACATAGGGA ATCCAGTGTT
    AGTCAAGCCA GTGACAGTGA GGGACACACC AGAGATTCAG GTAGGCATTC TGAGACAACT
    CATGGAAGGC CTGGTTCAAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGTACTCAGC ATATCATCAA GGGAGGCACA GTGAATCTGT CCATGAAAGG
    TCAGCGTCTA GAACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACAGTCTG GAACTGGACA TGGACATGCC TCTGCTGGAT CTGGAATCAA CAGACATAGG
    GAATCCAGTG TTAGTCAGGC CAGTGACAGA GAGGGACATG CAAGAGATTC AGGTAGACAG
    TCCGTGACAA CTCATGGAAG GCCTGGTTCC AGCTCAAGAA ACCAACATGT ATCTTCCCAT
    GGCCAGATAG GAGACAGTTC TAGCCACTCA GAGACCAATC AAGGGAGCCA CAATGAGAGA
    CATACAAGAG ATTCAGGTAG GCATTCCGAG ACAACTCATG GAAGACTTGG TTCCAGCTCA
    AGGAACCAAC ATGGATCTGT CCATGGCCAG ACAAGAGACA GTCCTAGGCA CTCAGAGTCC
    CATCAAGAGA GGCACAGTGA ATCTGTCCAT GAAAGGTCAG GATCCAGCAC TGGACAAAGA
    CAGGGGAGCC ACCATGAGCA GTCAAGAGAC AGCTCCAGAC ACTCTGGAAC TCGACATGGA
    CAAACCTCTA CTGTATCTGG AGGCAGTAGG CACAGGGAAC CCAGCGTTAG TCAGGCCAGT
    GACAGTGAGG GACATTCAGA AGACTCAGGT AGACAGTCCG TGACAACTCA TGGAAGGCCT
    GGTTCAACTT CAAGAAACCA ACATGGATCT GTCCATGGCC AGACAAGAGA CAGTCCTAGG
    CACTCAGAGT CCCATCAAGA GAGGCACAGT GAATCTGTCC ACCAAAGGTC TGGATCAAGC
    ACAGGGCAAA GACAGGGGAG CCACCATGAG CAGTCAAGAG ACAGCTCCAG ACACTCTGGA
    ACCGGTCATG GACAAACCTC CGCTGGATCC GGAAGTAGTA GGCACAGGGA ATCCAGTGTT
    AGTCAGGCCA GTGACAGTGA AGGACATTCA GAAGACTCAG GTAGACAGAA CGTGACAACT
    CATGGAAGGC CTGGTTCCAG CTCAAGAAAC CAACATGGAT CTTCCCAGGG CCAGACAGGA
    GACAGTTCTA GGTACTCAGC ATCTCATCAA GGGAGGCACA GTGAATCTGT CCATGAAAGG
    TCAACATCTA GAACTGGGCA AAGACAGGGG AGCCACCATG AGCAGTCAAG GGACAGCTCC
    AGACAGTCTG GAACTGGACA TGGACATGCC TCTGCTGGAT CTGGAATCAA CAGACATAGG
    GAATCCAGTG TTAGTCAGGC CAGTGACAGT GAAGGACATT CAGAAGACTC AGGCAGACAG
    AACGTGACAA CTCATGGAAG GCCCGGTTCC AGCTCAAGCA ATCAACATGG ATCTTCCCAT
    GGCCAGACTG GAGACAGTTC TAGGCACTCA GAGTCCCATC AAGGGAGCCA CGGTGAATCT
    GTCCACCAAA GGTCTGGATC AAGCACTGGG CAAAGACAGG GGAGCCACCA TGAGCAGTCA
    AGGGACAGCT CCAGACACTC TGGAACTGGT CATGGAGAAA CATCCACTGG ATCTGGAAGC
    AGCAGACATA GGGAATCCAG TGTTAGTCAG TCCAGTGACA GCAAGGGTCA TTCAAGAGGT
    TCAGGTAATC AGACTGTGAC CAACCAAAGA ATGTCTGCAT TCTACTCAAG GTTCCAAAAT
    CATGGTGCAG GCCAAGTTTG GAGACATGGC AGCTATGGCA GTTCCAATTA TGACTATGGA
    CAGTTAGGGA CTGGACATTC TCCAGATGAA AATTTTAGTC ACGATTCCAG TCATGTGGAA
    AAGAGGGACA AACCTGAATA TAGAGGAGAA CTAATGAGAT CCAATATAAC AGTCCGTAAT
    ATACACCCTG GAACTTATGG CCATTCAAAT TATATTTCAA AACAGCTGGG ATTTGGACAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90409
    Clone ID Related Accession (Same CDS sequence) XM_010803068.2
    Accession Version XM_010803068.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product filaggrin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103863.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803068.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGAAGGCC TGGTTCCAGC TCAAGAAATC AACATGGATC TTCCCATGGC CAGACTGGAG 
    ACAGTTCTAG GCATTCAGAG TCCCATCAAA GAAGCCACAG TGAAGCTGTT CATGAAAGGT
    CAGGATCCAG CACGGGGCAA AGGCAGAGGA GCCACCATGA GCAGTCAAGG GACAGCTCCA
    GACACTCTGG AACTGGACAT GGACAAATCT CAGCTGAATC TGGAAATGGC AGACATAGGG
    AATCCAGTGT TAGTCAAGCC AGTGACAGTG AGGGACACGC CAGAGATTCA GGTAGGCATT
    CTGAGACAAC TCATGGAAGA TATGGTTCCA GCTCAAGGAA CCAACATGGA TCCTCCCATG
    GTCAGACAGG GGACAGTTAT AGGCACTCAG AGACCAATCA AGGGAGACAC AATAAGAGAC
    ACACAAGAGA TTCAGGTAGG CATTCTGAGA CAACTCATGG AAGACCTGGT TCCAGCTCAA
    GAAACCAACA TGGATCTTCC CATGGCCAGA GTGGAGACAG TTCTAGGCAC TCAGAGTCCC
    ATCAAGAGAG GCATAGTGAA TCTGTCCATG GAAGGTCAGG ATCCAGCACT GGGCAAAGAC
    AGGGGAGCCA CCATGAGCAG TCAAGAGACA GCTCCAGACA CTCTGGAACT CGACATGGGC
    AAACCTCAGC TGAATCTGGA AATGGCAGAC ATAGGGAATC CAGTGTTAGT CAAGCCAGTG
    ACAGTGAGGG ACACACCAGA GATTCAGGTA GGCATTCTGA GACAACTCAT GGAAGGCCTG
    GTTCAAGCTC AAGAAACCAA CATGGATCTT CCCAGGGCCA GACAGGAGAC AGTTCTAGGT
    ACTCAGCATA TCATCAAGGG AGGCACAGTG AATCTGTCCA TGAAAGGTCA GCGTCTAGAA
    CTGGGCAAAG ACAGGGGAGC CACCATGAGC AGTCAAGGGA CAGCTCCAGA CAGTCTGGAA
    CTGGACATGG ACATACCTCT GCTGGATCTG GAAATGGCAG ACATAGGGAA TCCAGTGTTA
    GTCAGTCAAG TGACAGAGAG GGACACGCAA GAGATTCAGG TAGACAGTCC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010801370.2
    CDS1549..2622
    Translation

    Target ORF information:

    RefSeq Version XM_010803068.2
    Organism Bos taurus(cattle)
    Definition Bos taurus filaggrin (FLG), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010803068.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGAAGGCC TGGTTCCAGC TCAAGAAATC AACATGGATC TTCCCATGGC CAGACTGGAG 
    ACAGTTCTAG GCATTCAGAG TCCCATCAAA GAAGCCACAG TGAAGCTGTT CATGAAAGGT
    CAGGATCCAG CACGGGGCAA AGGCAGAGGA GCCACCATGA GCAGTCAAGG GACAGCTCCA
    GACACTCTGG AACTGGACAT GGACAAATCT CAGCTGAATC TGGAAATGGC AGACATAGGG
    AATCCAGTGT TAGTCAAGCC AGTGACAGTG AGGGACACGC CAGAGATTCA GGTAGGCATT
    CTGAGACAAC TCATGGAAGA TATGGTTCCA GCTCAAGGAA CCAACATGGA TCCTCCCATG
    GTCAGACAGG GGACAGTTAT AGGCACTCAG AGACCAATCA AGGGAGACAC AATAAGAGAC
    ACACAAGAGA TTCAGGTAGG CATTCTGAGA CAACTCATGG AAGACCTGGT TCCAGCTCAA
    GAAACCAACA TGGATCTTCC CATGGCCAGA GTGGAGACAG TTCTAGGCAC TCAGAGTCCC
    ATCAAGAGAG GCATAGTGAA TCTGTCCATG GAAGGTCAGG ATCCAGCACT GGGCAAAGAC
    AGGGGAGCCA CCATGAGCAG TCAAGAGACA GCTCCAGACA CTCTGGAACT CGACATGGGC
    AAACCTCAGC TGAATCTGGA AATGGCAGAC ATAGGGAATC CAGTGTTAGT CAAGCCAGTG
    ACAGTGAGGG ACACACCAGA GATTCAGGTA GGCATTCTGA GACAACTCAT GGAAGGCCTG
    GTTCAAGCTC AAGAAACCAA CATGGATCTT CCCAGGGCCA GACAGGAGAC AGTTCTAGGT
    ACTCAGCATA TCATCAAGGG AGGCACAGTG AATCTGTCCA TGAAAGGTCA GCGTCTAGAA
    CTGGGCAAAG ACAGGGGAGC CACCATGAGC AGTCAAGGGA CAGCTCCAGA CAGTCTGGAA
    CTGGACATGG ACATACCTCT GCTGGATCTG GAAATGGCAG ACATAGGGAA TCCAGTGTTA
    GTCAGTCAAG TGACAGAGAG GGACACGCAA GAGATTCAGG TAGACAGTCC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.