PJA1 cDNA ORF clone, Bos taurus(cattle)

The following PJA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PJA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa02125 NM_001080366.1
Latest version!
Bos taurus praja ring finger ubiquitin ligase 1 (PJA1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa02125 XM_024988340.1
Latest version!
Bos taurus praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa238525 XM_024988341.1
Latest version!
Bos taurus praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa238526 XM_024988342.1
Latest version!
Bos taurus praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa35504 XM_015470432.1
Latest version!
Bos taurus praja ring finger 1, E3 ubiquitin protein ligase (PJA1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OBa35504 XM_002700048.5
Latest version!
Bos taurus praja ring finger 1, E3 ubiquitin protein ligase (PJA1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa02125
    Clone ID Related Accession (Same CDS sequence) XM_024988340.1 , NM_001080366.1
    Accession Version NM_001080366.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase Praja-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BT026528.1. On Jan 22, 2007 this sequence version replaced XM_001254246.1. ##Evidence-Data-START## Transcript exon combination :: BT026528.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GATCACCGTT TTCCACCACT 
    CGTCGTAGTT GGGATGACAG CGAGAGCTCA GGAACCAGCC TGAACGCTGA TAATGAGGAC
    TACTCCAGGT ACCCGCCCAG AGAGTACAGG GCTTCGGGGA GCAGAAGAGG AATGGCTTAT
    GGACATGTTG ACTGTTTCGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG
    CGAGTGTCAG TGAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCTGTA TGACCCGGAG
    AAGGGGGCAA GGTCTCTGGC TGGGGTGGCC TCACAGTTCT CTAGTTTTAA CCATGATGTG
    AGAGAGGAGC TTGACAAGTT AGACCCAGCC CCTGCAGCAC GGTCCTCTGC TAGCAGAGCT
    GAGTTCTTGC AGTCAAATAG CATGGCCTCT CAGCCGTCTT CTGCTGAAGG CAAGGTGGTC
    ACAAACAGCA ACAACCTGGA GAGGGAGAGA CAGGAGCCGA ATTTACCTGC ATGTCCCAGC
    AGGGCTCCTG TGAGTATTTG TGGTGGGGAA AACACTCCCA AGAGCGCAGA GGAACCGGTG
    GTGAGGCCTA AAATCAGAAA TCTGGCAAGT CCCAACTGCG TGAAACCAAA AATCTTTTTT
    GATACCGATG ATGATGATGA TATGCCACAT AGCACTTCCA GGTGGAGGGA GACTGCCAGC
    GCTGATGAAG GCCACTTGGA TGGCCTGGCA AGAAGGAGCG GAGGTGAGGG CTCAAGTGGC
    TACCCTGAGC CGAAGTACCC CGAAGACAAG AGGGAAGCCA GGAGTGACCA AGTGAAGCCC
    GAAAAGGTGC CTAGACGGCG ACGAACCATG GCCGACCCCG ACTTCTGGAC ATACAGCGAT
    GACTACTACA AATACTTTGA AGAAGACTCT GACAGTGACA AAGAGTGGAC TGCGGCTCTG
    CGTCGCAAGT ATCGTGGTCG GGAGCAAAAT CTGTCGTCCA GCGGCGAGAG CTGGGAGACT
    CTGCCGGGAA AAGAAGAGCT TGAAGCCGAG CAAGCCAGAG TGAATGCCAG TGCTGGTGCC
    AGTGCTGGCA CCAGTGGCAA CAATGAACTT GAAGAAGTTC GAGGGCCATC TCTCCAGGAA
    GAGGAAAGGG CATCCCCCGA AGAAGGAGAA GTTCCTTGGC TCCAATACAA TGAAAACGAG
    AGCAGCAGTG AGGGGGATAA TGATTCTGGT CAGGAGTTTC TGCAGCCTGG TGTCTTCATG
    CTGGATGGCA ACAACAACCT TGAAGATGAC TCTAGTGTCA GTGAAGACCT CGAAGTGGAT
    TGGAGCCTCT TCGATGGGTT TGCGGATGGG TTGGGGGTAG CTGAAGCCAT TTCCTACGTG
    GATCCTCAGT TCCTCACATA CATGGCACTT GAAGAACGCC TGGCCCAGGC AATGGAAACT
    GCCTTGGCAC ACTTGGAGTC TCTGGCGGTG GATGTGGAGG TGGCCAATCC ACCAGCCAGC
    AAGGAAAGCA TCGACACTCT TCCTGAGATC CTGATCACGG AAGATCACAG TGCGGTGGGG
    CAGGAGATGT GTTGCCCCAT CTGTTGCAGT GAATATGCGA AGGGGGAGGT GGCGACGGAG
    CTGCCATGCC ACCACTATTT CCACAAGCCT TGCGTGTCCA TCTGGCTTCA GAAGTCAGGC
    ACCTGCCCCG TGTGCCGCTG CATGTTCCCT CCCCCACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001073835.1
    CDS150..1871
    Translation

    Target ORF information:

    RefSeq Version NM_001080366.1
    Organism Bos taurus(cattle)
    Definition Bos taurus praja ring finger ubiquitin ligase 1 (PJA1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001080366.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GATCACCGTT TTCCACCACT 
    CGTCGTAGTT GGGATGACAG CGAGAGCTCA GGAACCAGCC TGAACGCTGA TAATGAGGAC
    TACTCCAGGT ACCCGCCCAG AGAGTACAGG GCTTCGGGGA GCAGAAGAGG AATGGCTTAT
    GGACATGTTG ACTGTTTCGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG
    CGAGTGTCAG TGAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCTGTA TGACCCGGAG
    AAGGGGGCAA GGTCTCTGGC TGGGGTGGCC TCACAGTTCT CTAGTTTTAA CCATGATGTG
    AGAGAGGAGC TTGACAAGTT AGACCCAGCC CCTGCAGCAC GGTCCTCTGC TAGCAGAGCT
    GAGTTCTTGC AGTCAAATAG CATGGCCTCT CAGCCGTCTT CTGCTGAAGG CAAGGTGGTC
    ACAAACAGCA ACAACCTGGA GAGGGAGAGA CAGGAGCCGA ATTTACCTGC ATGTCCCAGC
    AGGGCTCCTG TGAGTATTTG TGGTGGGGAA AACACTCCCA AGAGCGCAGA GGAACCGGTG
    GTGAGGCCTA AAATCAGAAA TCTGGCAAGT CCCAACTGCG TGAAACCAAA AATCTTTTTT
    GATACCGATG ATGATGATGA TATGCCACAT AGCACTTCCA GGTGGAGGGA GACTGCCAGC
    GCTGATGAAG GCCACTTGGA TGGCCTGGCA AGAAGGAGCG GAGGTGAGGG CTCAAGTGGC
    TACCCTGAGC CGAAGTACCC CGAAGACAAG AGGGAAGCCA GGAGTGACCA AGTGAAGCCC
    GAAAAGGTGC CTAGACGGCG ACGAACCATG GCCGACCCCG ACTTCTGGAC ATACAGCGAT
    GACTACTACA AATACTTTGA AGAAGACTCT GACAGTGACA AAGAGTGGAC TGCGGCTCTG
    CGTCGCAAGT ATCGTGGTCG GGAGCAAAAT CTGTCGTCCA GCGGCGAGAG CTGGGAGACT
    CTGCCGGGAA AAGAAGAGCT TGAAGCCGAG CAAGCCAGAG TGAATGCCAG TGCTGGTGCC
    AGTGCTGGCA CCAGTGGCAA CAATGAACTT GAAGAAGTTC GAGGGCCATC TCTCCAGGAA
    GAGGAAAGGG CATCCCCCGA AGAAGGAGAA GTTCCTTGGC TCCAATACAA TGAAAACGAG
    AGCAGCAGTG AGGGGGATAA TGATTCTGGT CAGGAGTTTC TGCAGCCTGG TGTCTTCATG
    CTGGATGGCA ACAACAACCT TGAAGATGAC TCTAGTGTCA GTGAAGACCT CGAAGTGGAT
    TGGAGCCTCT TCGATGGGTT TGCGGATGGG TTGGGGGTAG CTGAAGCCAT TTCCTACGTG
    GATCCTCAGT TCCTCACATA CATGGCACTT GAAGAACGCC TGGCCCAGGC AATGGAAACT
    GCCTTGGCAC ACTTGGAGTC TCTGGCGGTG GATGTGGAGG TGGCCAATCC ACCAGCCAGC
    AAGGAAAGCA TCGACACTCT TCCTGAGATC CTGATCACGG AAGATCACAG TGCGGTGGGG
    CAGGAGATGT GTTGCCCCAT CTGTTGCAGT GAATATGCGA AGGGGGAGGT GGCGACGGAG
    CTGCCATGCC ACCACTATTT CCACAAGCCT TGCGTGTCCA TCTGGCTTCA GAAGTCAGGC
    ACCTGCCCCG TGTGCCGCTG CATGTTCCCT CCCCCACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02125
    Clone ID Related Accession (Same CDS sequence) XM_024988340.1 , NM_001080366.1
    Accession Version XM_024988340.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase Praja-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GATCACCGTT TTCCACCACT 
    CGTCGTAGTT GGGATGACAG CGAGAGCTCA GGAACCAGCC TGAACGCTGA TAATGAGGAC
    TACTCCAGGT ACCCGCCCAG AGAGTACAGG GCTTCGGGGA GCAGAAGAGG AATGGCTTAT
    GGACATGTTG ACTGTTTCGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG
    CGAGTGTCAG TGAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCTGTA TGACCCGGAG
    AAGGGGGCAA GGTCTCTGGC TGGGGTGGCC TCACAGTTCT CTAGTTTTAA CCATGATGTG
    AGAGAGGAGC TTGACAAGTT AGACCCAGCC CCTGCAGCAC GGTCCTCTGC TAGCAGAGCT
    GAGTTCTTGC AGTCAAATAG CATGGCCTCT CAGCCGTCTT CTGCTGAAGG CAAGGTGGTC
    ACAAACAGCA ACAACCTGGA GAGGGAGAGA CAGGAGCCGA ATTTACCTGC ATGTCCCAGC
    AGGGCTCCTG TGAGTATTTG TGGTGGGGAA AACACTCCCA AGAGCGCAGA GGAACCGGTG
    GTGAGGCCTA AAATCAGAAA TCTGGCAAGT CCCAACTGCG TGAAACCAAA AATCTTTTTT
    GATACCGATG ATGATGATGA TATGCCACAT AGCACTTCCA GGTGGAGGGA GACTGCCAGC
    GCTGATGAAG GCCACTTGGA TGGCCTGGCA AGAAGGAGCG GAGGTGAGGG CTCAAGTGGC
    TACCCTGAGC CGAAGTACCC CGAAGACAAG AGGGAAGCCA GGAGTGACCA AGTGAAGCCC
    GAAAAGGTGC CTAGACGGCG ACGAACCATG GCCGACCCCG ACTTCTGGAC ATACAGCGAT
    GACTACTACA AATACTTTGA AGAAGACTCT GACAGTGACA AAGAGTGGAC TGCGGCTCTG
    CGTCGCAAGT ATCGTGGTCG GGAGCAAAAT CTGTCGTCCA GCGGCGAGAG CTGGGAGACT
    CTGCCGGGAA AAGAAGAGCT TGAAGCCGAG CAAGCCAGAG TGAATGCCAG TGCTGGTGCC
    AGTGCTGGCA CCAGTGGCAA CAATGAACTT GAAGAAGTTC GAGGGCCATC TCTCCAGGAA
    GAGGAAAGGG CATCCCCCGA AGAAGGAGAA GTTCCTTGGC TCCAATACAA TGAAAACGAG
    AGCAGCAGTG AGGGGGATAA TGATTCTGGT CAGGAGTTTC TGCAGCCTGG TGTCTTCATG
    CTGGATGGCA ACAACAACCT TGAAGATGAC TCTAGTGTCA GTGAAGACCT CGAAGTGGAT
    TGGAGCCTCT TCGATGGGTT TGCGGATGGG TTGGGGGTAG CTGAAGCCAT TTCCTACGTG
    GATCCTCAGT TCCTCACATA CATGGCACTT GAAGAACGCC TGGCCCAGGC AATGGAAACT
    GCCTTGGCAC ACTTGGAGTC TCTGGCGGTG GATGTGGAGG TGGCCAATCC ACCAGCCAGC
    AAGGAAAGCA TCGACACTCT TCCTGAGATC CTGATCACGG AAGATCACAG TGCGGTGGGG
    CAGGAGATGT GTTGCCCCAT CTGTTGCAGT GAATATGCGA AGGGGGAGGT GGCGACGGAG
    CTGCCATGCC ACCACTATTT CCACAAGCCT TGCGTGTCCA TCTGGCTTCA GAAGTCAGGC
    ACCTGCCCCG TGTGCCGCTG CATGTTCCCT CCCCCACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844108.1
    CDS210..1931
    Translation

    Target ORF information:

    RefSeq Version XM_024988340.1
    Organism Bos taurus(cattle)
    Definition Bos taurus praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988340.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GATCACCGTT TTCCACCACT 
    CGTCGTAGTT GGGATGACAG CGAGAGCTCA GGAACCAGCC TGAACGCTGA TAATGAGGAC
    TACTCCAGGT ACCCGCCCAG AGAGTACAGG GCTTCGGGGA GCAGAAGAGG AATGGCTTAT
    GGACATGTTG ACTGTTTCGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG
    CGAGTGTCAG TGAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCTGTA TGACCCGGAG
    AAGGGGGCAA GGTCTCTGGC TGGGGTGGCC TCACAGTTCT CTAGTTTTAA CCATGATGTG
    AGAGAGGAGC TTGACAAGTT AGACCCAGCC CCTGCAGCAC GGTCCTCTGC TAGCAGAGCT
    GAGTTCTTGC AGTCAAATAG CATGGCCTCT CAGCCGTCTT CTGCTGAAGG CAAGGTGGTC
    ACAAACAGCA ACAACCTGGA GAGGGAGAGA CAGGAGCCGA ATTTACCTGC ATGTCCCAGC
    AGGGCTCCTG TGAGTATTTG TGGTGGGGAA AACACTCCCA AGAGCGCAGA GGAACCGGTG
    GTGAGGCCTA AAATCAGAAA TCTGGCAAGT CCCAACTGCG TGAAACCAAA AATCTTTTTT
    GATACCGATG ATGATGATGA TATGCCACAT AGCACTTCCA GGTGGAGGGA GACTGCCAGC
    GCTGATGAAG GCCACTTGGA TGGCCTGGCA AGAAGGAGCG GAGGTGAGGG CTCAAGTGGC
    TACCCTGAGC CGAAGTACCC CGAAGACAAG AGGGAAGCCA GGAGTGACCA AGTGAAGCCC
    GAAAAGGTGC CTAGACGGCG ACGAACCATG GCCGACCCCG ACTTCTGGAC ATACAGCGAT
    GACTACTACA AATACTTTGA AGAAGACTCT GACAGTGACA AAGAGTGGAC TGCGGCTCTG
    CGTCGCAAGT ATCGTGGTCG GGAGCAAAAT CTGTCGTCCA GCGGCGAGAG CTGGGAGACT
    CTGCCGGGAA AAGAAGAGCT TGAAGCCGAG CAAGCCAGAG TGAATGCCAG TGCTGGTGCC
    AGTGCTGGCA CCAGTGGCAA CAATGAACTT GAAGAAGTTC GAGGGCCATC TCTCCAGGAA
    GAGGAAAGGG CATCCCCCGA AGAAGGAGAA GTTCCTTGGC TCCAATACAA TGAAAACGAG
    AGCAGCAGTG AGGGGGATAA TGATTCTGGT CAGGAGTTTC TGCAGCCTGG TGTCTTCATG
    CTGGATGGCA ACAACAACCT TGAAGATGAC TCTAGTGTCA GTGAAGACCT CGAAGTGGAT
    TGGAGCCTCT TCGATGGGTT TGCGGATGGG TTGGGGGTAG CTGAAGCCAT TTCCTACGTG
    GATCCTCAGT TCCTCACATA CATGGCACTT GAAGAACGCC TGGCCCAGGC AATGGAAACT
    GCCTTGGCAC ACTTGGAGTC TCTGGCGGTG GATGTGGAGG TGGCCAATCC ACCAGCCAGC
    AAGGAAAGCA TCGACACTCT TCCTGAGATC CTGATCACGG AAGATCACAG TGCGGTGGGG
    CAGGAGATGT GTTGCCCCAT CTGTTGCAGT GAATATGCGA AGGGGGAGGT GGCGACGGAG
    CTGCCATGCC ACCACTATTT CCACAAGCCT TGCGTGTCCA TCTGGCTTCA GAAGTCAGGC
    ACCTGCCCCG TGTGCCGCTG CATGTTCCCT CCCCCACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238525
    Clone ID Related Accession (Same CDS sequence) XM_024988341.1
    Accession Version XM_024988341.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase Praja-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGGTCAGG AATCTAGCAA GCCTGTCTGG CCCAAGCCAG CAGGAGGGTG TCAGTCCAAT 
    ACAGGCAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCATC CACAAGCCAG
    CAAGAAAGGA TTTCCAGGCA GACAAAGACG CCATCCGAAG TCCCAATGCA CAGATCAGCC
    CCCAGTCAAA CCACCAAGAG GAGCCGATCA CCGTTTTCCA CCACTCGTCG TAGTTGGGAT
    GACAGCGAGA GCTCAGGAAC CAGCCTGAAC GCTGATAATG AGGACTACTC CAGCACTTCC
    AGGTGGAGGG AGACTGCCAG CGCTGATGAA GGCCACTTGG ATGGCCTGGC AAGAAGGAGC
    GGAGGTGAGG GCTCAAGTGG CTACCCTGAG CCGAAGTACC CCGAAGACAA GAGGGAAGCC
    AGGAGTGACC AAGTGAAGCC CGAAAAGGTG CCTAGACGGC GACGAACCAT GGCCGACCCC
    GACTTCTGGA CATACAGCGA TGACTACTAC AAATACTTTG AAGAAGACTC TGACAGTGAC
    AAAGAGTGGA CTGCGGCTCT GCGTCGCAAG TATCGTGGTC GGGAGCAAAA TCTGTCGTCC
    AGCGGCGAGA GCTGGGAGAC TCTGCCGGGA AAAGAAGAGC TTGAAGCCGA GCAAGCCAGA
    GTGAATGCCA GTGCTGGTGC CAGTGCTGGC ACCAGTGGCA ACAATGAACT TGAAGAAGTT
    CGAGGGCCAT CTCTCCAGGA AGAGGAAAGG GCATCCCCCG AAGAAGGAGA AGTTCCTTGG
    CTCCAATACA ATGAAAACGA GAGCAGCAGT GAGGGGGATA ATGATTCTGG TCAGGAGTTT
    CTGCAGCCTG GTGTCTTCAT GCTGGATGGC AACAACAACC TTGAAGATGA CTCTAGTGTC
    AGTGAAGACC TCGAAGTGGA TTGGAGCCTC TTCGATGGGT TTGCGGATGG GTTGGGGGTA
    GCTGAAGCCA TTTCCTACGT GGATCCTCAG TTCCTCACAT ACATGGCACT TGAAGAACGC
    CTGGCCCAGG CAATGGAAAC TGCCTTGGCA CACTTGGAGT CTCTGGCGGT GGATGTGGAG
    GTGGCCAATC CACCAGCCAG CAAGGAAAGC ATCGACACTC TTCCTGAGAT CCTGATCACG
    GAAGATCACA GTGCGGTGGG GCAGGAGATG TGTTGCCCCA TCTGTTGCAG TGAATATGCG
    AAGGGGGAGG TGGCGACGGA GCTGCCATGC CACCACTATT TCCACAAGCC TTGCGTGTCC
    ATCTGGCTTC AGAAGTCAGG CACCTGCCCC GTGTGCCGCT GCATGTTCCC TCCCCCACTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844109.1
    CDS1390..2712
    Translation

    Target ORF information:

    RefSeq Version XM_024988341.1
    Organism Bos taurus(cattle)
    Definition Bos taurus praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988341.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGGTCAGG AATCTAGCAA GCCTGTCTGG CCCAAGCCAG CAGGAGGGTG TCAGTCCAAT 
    ACAGGCAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCATC CACAAGCCAG
    CAAGAAAGGA TTTCCAGGCA GACAAAGACG CCATCCGAAG TCCCAATGCA CAGATCAGCC
    CCCAGTCAAA CCACCAAGAG GAGCCGATCA CCGTTTTCCA CCACTCGTCG TAGTTGGGAT
    GACAGCGAGA GCTCAGGAAC CAGCCTGAAC GCTGATAATG AGGACTACTC CAGCACTTCC
    AGGTGGAGGG AGACTGCCAG CGCTGATGAA GGCCACTTGG ATGGCCTGGC AAGAAGGAGC
    GGAGGTGAGG GCTCAAGTGG CTACCCTGAG CCGAAGTACC CCGAAGACAA GAGGGAAGCC
    AGGAGTGACC AAGTGAAGCC CGAAAAGGTG CCTAGACGGC GACGAACCAT GGCCGACCCC
    GACTTCTGGA CATACAGCGA TGACTACTAC AAATACTTTG AAGAAGACTC TGACAGTGAC
    AAAGAGTGGA CTGCGGCTCT GCGTCGCAAG TATCGTGGTC GGGAGCAAAA TCTGTCGTCC
    AGCGGCGAGA GCTGGGAGAC TCTGCCGGGA AAAGAAGAGC TTGAAGCCGA GCAAGCCAGA
    GTGAATGCCA GTGCTGGTGC CAGTGCTGGC ACCAGTGGCA ACAATGAACT TGAAGAAGTT
    CGAGGGCCAT CTCTCCAGGA AGAGGAAAGG GCATCCCCCG AAGAAGGAGA AGTTCCTTGG
    CTCCAATACA ATGAAAACGA GAGCAGCAGT GAGGGGGATA ATGATTCTGG TCAGGAGTTT
    CTGCAGCCTG GTGTCTTCAT GCTGGATGGC AACAACAACC TTGAAGATGA CTCTAGTGTC
    AGTGAAGACC TCGAAGTGGA TTGGAGCCTC TTCGATGGGT TTGCGGATGG GTTGGGGGTA
    GCTGAAGCCA TTTCCTACGT GGATCCTCAG TTCCTCACAT ACATGGCACT TGAAGAACGC
    CTGGCCCAGG CAATGGAAAC TGCCTTGGCA CACTTGGAGT CTCTGGCGGT GGATGTGGAG
    GTGGCCAATC CACCAGCCAG CAAGGAAAGC ATCGACACTC TTCCTGAGAT CCTGATCACG
    GAAGATCACA GTGCGGTGGG GCAGGAGATG TGTTGCCCCA TCTGTTGCAG TGAATATGCG
    AAGGGGGAGG TGGCGACGGA GCTGCCATGC CACCACTATT TCCACAAGCC TTGCGTGTCC
    ATCTGGCTTC AGAAGTCAGG CACCTGCCCC GTGTGCCGCT GCATGTTCCC TCCCCCACTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238526
    Clone ID Related Accession (Same CDS sequence) XM_024988342.1
    Accession Version XM_024988342.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase Praja-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GATCACCGTT TTCCACCACT 
    CGTCGTAGTT GGGATGACAG CGAGAGCTCA GGAACCAGCC TGAACGCTGA TAATGAGGAC
    TACTCCAGCA CTTCCAGGTG GAGGGAGACT GCCAGCGCTG ATGAAGGCCA CTTGGATGGC
    CTGGCAAGAA GGAGCGGAGG TGAGGGCTCA AGTGGCTACC CTGAGCCGAA GTACCCCGAA
    GACAAGAGGG AAGCCAGGAG TGACCAAGTG AAGCCCGAAA AGGTGCCTAG ACGGCGACGA
    ACCATGGCCG ACCCCGACTT CTGGACATAC AGCGATGACT ACTACAAATA CTTTGAAGAA
    GACTCTGACA GTGACAAAGA GTGGACTGCG GCTCTGCGTC GCAAGTATCG TGGTCGGGAG
    CAAAATCTGT CGTCCAGCGG CGAGAGCTGG GAGACTCTGC CGGGAAAAGA AGAGCTTGAA
    GCCGAGCAAG CCAGAGTGAA TGCCAGTGCT GGTGCCAGTG CTGGCACCAG TGGCAACAAT
    GAACTTGAAG AAGTTCGAGG GCCATCTCTC CAGGAAGAGG AAAGGGCATC CCCCGAAGAA
    GGAGAAGTTC CTTGGCTCCA ATACAATGAA AACGAGAGCA GCAGTGAGGG GGATAATGAT
    TCTGGTCAGG AGTTTCTGCA GCCTGGTGTC TTCATGCTGG ATGGCAACAA CAACCTTGAA
    GATGACTCTA GTGTCAGTGA AGACCTCGAA GTGGATTGGA GCCTCTTCGA TGGGTTTGCG
    GATGGGTTGG GGGTAGCTGA AGCCATTTCC TACGTGGATC CTCAGTTCCT CACATACATG
    GCACTTGAAG AACGCCTGGC CCAGGCAATG GAAACTGCCT TGGCACACTT GGAGTCTCTG
    GCGGTGGATG TGGAGGTGGC CAATCCACCA GCCAGCAAGG AAAGCATCGA CACTCTTCCT
    GAGATCCTGA TCACGGAAGA TCACAGTGCG GTGGGGCAGG AGATGTGTTG CCCCATCTGT
    TGCAGTGAAT ATGCGAAGGG GGAGGTGGCG ACGGAGCTGC CATGCCACCA CTATTTCCAC
    AAGCCTTGCG TGTCCATCTG GCTTCAGAAG TCAGGCACCT GCCCCGTGTG CCGCTGCATG
    TTCCCTCCCC CACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844110.1
    CDS220..1377
    Translation

    Target ORF information:

    RefSeq Version XM_024988342.1
    Organism Bos taurus(cattle)
    Definition Bos taurus praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988342.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GATCACCGTT TTCCACCACT 
    CGTCGTAGTT GGGATGACAG CGAGAGCTCA GGAACCAGCC TGAACGCTGA TAATGAGGAC
    TACTCCAGCA CTTCCAGGTG GAGGGAGACT GCCAGCGCTG ATGAAGGCCA CTTGGATGGC
    CTGGCAAGAA GGAGCGGAGG TGAGGGCTCA AGTGGCTACC CTGAGCCGAA GTACCCCGAA
    GACAAGAGGG AAGCCAGGAG TGACCAAGTG AAGCCCGAAA AGGTGCCTAG ACGGCGACGA
    ACCATGGCCG ACCCCGACTT CTGGACATAC AGCGATGACT ACTACAAATA CTTTGAAGAA
    GACTCTGACA GTGACAAAGA GTGGACTGCG GCTCTGCGTC GCAAGTATCG TGGTCGGGAG
    CAAAATCTGT CGTCCAGCGG CGAGAGCTGG GAGACTCTGC CGGGAAAAGA AGAGCTTGAA
    GCCGAGCAAG CCAGAGTGAA TGCCAGTGCT GGTGCCAGTG CTGGCACCAG TGGCAACAAT
    GAACTTGAAG AAGTTCGAGG GCCATCTCTC CAGGAAGAGG AAAGGGCATC CCCCGAAGAA
    GGAGAAGTTC CTTGGCTCCA ATACAATGAA AACGAGAGCA GCAGTGAGGG GGATAATGAT
    TCTGGTCAGG AGTTTCTGCA GCCTGGTGTC TTCATGCTGG ATGGCAACAA CAACCTTGAA
    GATGACTCTA GTGTCAGTGA AGACCTCGAA GTGGATTGGA GCCTCTTCGA TGGGTTTGCG
    GATGGGTTGG GGGTAGCTGA AGCCATTTCC TACGTGGATC CTCAGTTCCT CACATACATG
    GCACTTGAAG AACGCCTGGC CCAGGCAATG GAAACTGCCT TGGCACACTT GGAGTCTCTG
    GCGGTGGATG TGGAGGTGGC CAATCCACCA GCCAGCAAGG AAAGCATCGA CACTCTTCCT
    GAGATCCTGA TCACGGAAGA TCACAGTGCG GTGGGGCAGG AGATGTGTTG CCCCATCTGT
    TGCAGTGAAT ATGCGAAGGG GGAGGTGGCG ACGGAGCTGC CATGCCACCA CTATTTCCAC
    AAGCCTTGCG TGTCCATCTG GCTTCAGAAG TCAGGCACCT GCCCCGTGTG CCGCTGCATG
    TTCCCTCCCC CACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35504
    Clone ID Related Accession (Same CDS sequence) XM_002700048.5 , XM_015470432.1
    Accession Version XM_002700048.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase Praja-1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104728.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002700048.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 3 indels internal stop codons :: corrected 1 genomic stop codons ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GATCACCGTT TTCCACCACT 
    CGTCGTAGTT GGGATGACAG CGAGAGCTCA GGAACCAGCC TGAACGCTGA TAATGAGGAC
    TACTCCAGGT ACCCGCCCAG AGAGTACAGG GCTTCGGGGA GCAGAAGAGG AATGGCTTAT
    GGACATGTTG ACTGTTTCGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG
    CGAGTGTCAG TGAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCTGTA TGACCCGGAG
    AAGGGGGCAA GGTCTCTGGC TGGGGTGGCC TCACAGTTCT CTAGTTTTAA CCATGATGTG
    AGAGAGGAGC TTGACAAGTT AGACCCAGCC CCTGCAGCAC GGTCCTCTGC TAGCAGAGCT
    GAGTTCTTGC AGTCAAATAG CATGGCCTCT CAGCCGTCTT CTGCTGAAGG CAAGGTGGTC
    ACAAACAGCA ACAACCTGGA GAGGGAGAGA CAGGAGCCGA ATTTACCTGC ATGTCCCAGC
    AGGGCTCCTG TGAGTATTTG TGGTGGGGAA AACACTCCCA AGAGCGCAGA GGAACCGGTG
    GTGAGGCCTA AAATCAGAAA TCTGGCAAGT CCCAACTGCG TGAAACCAAA AATCTTTTTT
    GATACCGATG ATGATGATGA TATGCCACAT AGCACTTCCA GGTGGAGGGA GACTGCCAGC
    GCTGATGAAG GCCACTTGGA TGGCCTGGCA AGAAGANGCG GAGGTGAGGG CTCAAGTGGC
    TACCCTGAGC CGAAGTACCC CGAAGACAGA NNGGGAAGCC AGGAGTGACA AGTGAAGCCC
    GAAAAGGTGC CTAGACGGCG ACGAACCATG GCCGACCCCG ACTTCTGGAC ATACAGCGAT
    GACTACTACA AATACTTTGA NNNNNNGGCC AATCCACCAG CCAGCAAGGA AAGCATCGAC
    ACTCTTCCTG AGATCCTGAT CACGGAAGAT CACAGTGCGG TGGGGCAGGA GATGTGTTGC
    CCCATCTGTT GCAGTGAATA TGCGAAGGGG GAGGTGGCGA CGGAGCTGCC ATGCCACCAC
    TATTTCCACA AGCCTTGCGT GTCCATCTGG CTTCAGAAGT CAGGCACCTG CCCCGTGTGC
    CGCTGCATGT TCCCTCCCCC ACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002700094.1
    CDS215..1381
    Translation

    Target ORF information:

    RefSeq Version XM_002700048.5
    Organism Bos taurus(cattle)
    Definition Bos taurus praja ring finger 1, E3 ubiquitin protein ligase (PJA1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002700048.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GATCACCGTT TTCCACCACT 
    CGTCGTAGTT GGGATGACAG CGAGAGCTCA GGAACCAGCC TGAACGCTGA TAATGAGGAC
    TACTCCAGGT ACCCGCCCAG AGAGTACAGG GCTTCGGGGA GCAGAAGAGG AATGGCTTAT
    GGACATGTTG ACTGTTTCGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG
    CGAGTGTCAG TGAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCTGTA TGACCCGGAG
    AAGGGGGCAA GGTCTCTGGC TGGGGTGGCC TCACAGTTCT CTAGTTTTAA CCATGATGTG
    AGAGAGGAGC TTGACAAGTT AGACCCAGCC CCTGCAGCAC GGTCCTCTGC TAGCAGAGCT
    GAGTTCTTGC AGTCAAATAG CATGGCCTCT CAGCCGTCTT CTGCTGAAGG CAAGGTGGTC
    ACAAACAGCA ACAACCTGGA GAGGGAGAGA CAGGAGCCGA ATTTACCTGC ATGTCCCAGC
    AGGGCTCCTG TGAGTATTTG TGGTGGGGAA AACACTCCCA AGAGCGCAGA GGAACCGGTG
    GTGAGGCCTA AAATCAGAAA TCTGGCAAGT CCCAACTGCG TGAAACCAAA AATCTTTTTT
    GATACCGATG ATGATGATGA TATGCCACAT AGCACTTCCA GGTGGAGGGA GACTGCCAGC
    GCTGATGAAG GCCACTTGGA TGGCCTGGCA AGAAGANGCG GAGGTGAGGG CTCAAGTGGC
    TACCCTGAGC CGAAGTACCC CGAAGACAGA NNGGGAAGCC AGGAGTGACA AGTGAAGCCC
    GAAAAGGTGC CTAGACGGCG ACGAACCATG GCCGACCCCG ACTTCTGGAC ATACAGCGAT
    GACTACTACA AATACTTTGA NNNNNNGGCC AATCCACCAG CCAGCAAGGA AAGCATCGAC
    ACTCTTCCTG AGATCCTGAT CACGGAAGAT CACAGTGCGG TGGGGCAGGA GATGTGTTGC
    CCCATCTGTT GCAGTGAATA TGCGAAGGGG GAGGTGGCGA CGGAGCTGCC ATGCCACCAC
    TATTTCCACA AGCCTTGCGT GTCCATCTGG CTTCAGAAGT CAGGCACCTG CCCCGTGTGC
    CGCTGCATGT TCCCTCCCCC ACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35504
    Clone ID Related Accession (Same CDS sequence) XM_002700048.5 , XM_015470432.1
    Accession Version XM_015470432.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase Praja-1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647142.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 3 indels internal stop codons :: corrected 1 genomic stop codons ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GATCACCGTT TTCCACCACT 
    CGTCGTAGTT GGGATGACAG CGAGAGCTCA GGAACCAGCC TGAACGCTGA TAATGAGGAC
    TACTCCAGGT ACCCGCCCAG AGAGTACAGG GCTTCGGGGA GCAGAAGAGG AATGGCTTAT
    GGACATGTTG ACTGTTTCGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG
    CGAGTGTCAG TGAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCTGTA TGACCCGGAG
    AAGGGGGCAA GGTCTCTGGC TGGGGTGGCC TCACAGTTCT CTAGTTTTAA CCATGATGTG
    AGAGAGGAGC TTGACAAGTT AGACCCAGCC CCTGCAGCAC GGTCCTCTGC TAGCAGAGCT
    GAGTTCTTGC AGTCAAATAG CATGGCCTCT CAGCCGTCTT CTGCTGAAGG CAAGGTGGTC
    ACAAACAGCA ACAACCTGGA GAGGGAGAGA CAGGAGCCGA ATTTACCTGC ATGTCCCAGC
    AGGGCTCCTG TGAGTATTTG TGGTGGGGAA AACACTCCCA AGAGCGCAGA GGAACCGGTG
    GTGAGGCCTA AAATCAGAAA TCTGGCAAGT CCCAACTGCG TGAAACCAAA AATCTTTTTT
    GATACCGATG ATGATGATGA TATGCCACAT AGCACTTCCA GGTGGAGGGA GACTGCCAGC
    GCTGATGAAG GCCACTTGGA TGGCCTGGCA AGAAGANGCG GAGGTGAGGG CTCAAGTGGC
    TACCCTGAGC CGAAGTACCC CGAAGACAGA NNGGGAAGCC AGGAGTGACA AGTGAAGCCC
    GAAAAGGTGC CTAGACGGCG ACGAACCATG GCCGACCCCG ACTTCTGGAC ATACAGCGAT
    GACTACTACA AATACTTTGA NNNNNNGGCC AATCCACCAG CCAGCAAGGA AAGCATCGAC
    ACTCTTCCTG AGATCCTGAT CACGGAAGAT CACAGTGCGG TGGGGCAGGA GATGTGTTGC
    CCCATCTGTT GCAGTGAATA TGCGAAGGGG GAGGTGGCGA CGGAGCTGCC ATGCCACCAC
    TATTTCCACA AGCCTTGCGT GTCCATCTGG CTTCAGAAGT CAGGCACCTG CCCCGTGTGC
    CGCTGCATGT TCCCTCCCCC ACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325918.1
    CDS215..1381
    Translation

    Target ORF information:

    RefSeq Version XM_015470432.1
    Organism Bos taurus(cattle)
    Definition Bos taurus praja ring finger 1, E3 ubiquitin protein ligase (PJA1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470432.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GATCACCGTT TTCCACCACT 
    CGTCGTAGTT GGGATGACAG CGAGAGCTCA GGAACCAGCC TGAACGCTGA TAATGAGGAC
    TACTCCAGGT ACCCGCCCAG AGAGTACAGG GCTTCGGGGA GCAGAAGAGG AATGGCTTAT
    GGACATGTTG ACTGTTTCGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG
    CGAGTGTCAG TGAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCTGTA TGACCCGGAG
    AAGGGGGCAA GGTCTCTGGC TGGGGTGGCC TCACAGTTCT CTAGTTTTAA CCATGATGTG
    AGAGAGGAGC TTGACAAGTT AGACCCAGCC CCTGCAGCAC GGTCCTCTGC TAGCAGAGCT
    GAGTTCTTGC AGTCAAATAG CATGGCCTCT CAGCCGTCTT CTGCTGAAGG CAAGGTGGTC
    ACAAACAGCA ACAACCTGGA GAGGGAGAGA CAGGAGCCGA ATTTACCTGC ATGTCCCAGC
    AGGGCTCCTG TGAGTATTTG TGGTGGGGAA AACACTCCCA AGAGCGCAGA GGAACCGGTG
    GTGAGGCCTA AAATCAGAAA TCTGGCAAGT CCCAACTGCG TGAAACCAAA AATCTTTTTT
    GATACCGATG ATGATGATGA TATGCCACAT AGCACTTCCA GGTGGAGGGA GACTGCCAGC
    GCTGATGAAG GCCACTTGGA TGGCCTGGCA AGAAGANGCG GAGGTGAGGG CTCAAGTGGC
    TACCCTGAGC CGAAGTACCC CGAAGACAGA NNGGGAAGCC AGGAGTGACA AGTGAAGCCC
    GAAAAGGTGC CTAGACGGCG ACGAACCATG GCCGACCCCG ACTTCTGGAC ATACAGCGAT
    GACTACTACA AATACTTTGA NNNNNNGGCC AATCCACCAG CCAGCAAGGA AAGCATCGAC
    ACTCTTCCTG AGATCCTGAT CACGGAAGAT CACAGTGCGG TGGGGCAGGA GATGTGTTGC
    CCCATCTGTT GCAGTGAATA TGCGAAGGGG GAGGTGGCGA CGGAGCTGCC ATGCCACCAC
    TATTTCCACA AGCCTTGCGT GTCCATCTGG CTTCAGAAGT CAGGCACCTG CCCCGTGTGC
    CGCTGCATGT TCCCTCCCCC ACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.