HEATR5B gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005212707.2	XP_005212764.1	HEAT repeat-containing protein 5B isoform X2
XM_002691283.5	XP_002691329.1	HEAT repeat-containing protein 5B isoform X1
XM_005212707.3	XP_005212764.1	HEAT repeat-containing protein 5B isoform X2
XM_024999373.1	XP_024855141.1	HEAT repeat-containing protein 5B isoform X3
XM_005212706.3	XP_005212763.1	HEAT repeat-containing protein 5B isoform X1
XM_015465332.1	XP_015320818.1	HEAT repeat-containing protein 5B isoform X2
XM_002691283.6	XP_002691329.1	HEAT repeat-containing protein 5B isoform X1
XM_005212706.4	XP_005212763.1	HEAT repeat-containing protein 5B isoform X1
XM_015465330.1	XP_015320816.1	HEAT repeat-containing protein 5B isoform X1
XM_015465331.1	XP_015320817.1	HEAT repeat-containing protein 5B isoform X1
XM_024999374.1	XP_024855142.1	HEAT repeat-containing protein 5B isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa220802	XM_024999373.1 Latest version!	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa220803	XM_024999374.1 Latest version!	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa23993	XM_015465330.1 Latest version!	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23993	XM_015465331.1 Latest version!	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23993	XM_005212706.4 Latest version!	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23993	XM_002691283.6 Latest version!	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23994	XM_005212707.3 Latest version!	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23994	XM_015465332.1 Latest version!	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OBa23994	XM_005212707.2	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23993	XM_002691283.5	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa23993	XM_005212706.3	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

HEATR5B ( XM_024999373.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855141.1 Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa220802
Clone ID Related Accession (Same CDS sequence)	XM_024999373.1
Accession Version	XM_024999373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	HEAT repeat-containing protein 5B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTTCC TCCTCACTGC TGGAATCTCA AGGGAGTATG CTGGGACAAC TTGTCTCCTA 
GAACTACAGA ATGAAGCTGT GTTCATGTGG ACTGCTGAAC TGGAAAATAT AGCCACTCTC 
TGCTTCAAGG CTTTGGAAAA CTCTAACTAT GGGGTACGAG TGGCAGTGTC TAAGCTCTTA 
GGAACAGTCA TGGCTACAGC ACTGATGCCA AAACAAGCAA CAGTAATGCG CCAGAATGTG 
AAGCGAGCAA CCTTTGATGA AGTCTTAGAA CTCATGGCCA CAGGATTTCT GCGTGGAGGG 
TCAGGTTTCT TAAAGAGTGG TGGAGAAATG TTAAAAGTTG GAGGGTCTGT TAATCGTGAA 
GTGAGAGTTG GAGTTACCCA GGCATATGTT GTTTTTGTAA CAACATTGGG TGGCCAGTGG 
TTGGAGCGTA GTTTTGCTAC ATTCTTGTCT CATGTACTTG ATCTGGTTTC CCATCCTCGG 
GCAACACAAA CACATGTGGA GGCTGTGTAT TCCAGACGAT GTGTCTCCTT TATTTTAAGA 
GCTACCGTGG GCAGTTTGCT AGGTGAAAAA GCCCAGATTG CAGCTGCCAA AGAAATATGC 
CAGGCGATTG GAAAACAAAT GAAAGCAGTA GAAGCGGTAG TGAATGACAC TAGTGGGGAG 
AACAAGTCTG GAGCAGCAGA CATTGCAGCA AGCCAGCATG TGATGGTCTG TGCCCTGCAG 
GAGCTCGGGA GCCTGGTGCA GAGCCTGAAT GCCACCGCAT CCCCTCTTAT TCAGGAAGCA 
TCTATAGGCC TTTTGGAGAC TGTGACCTCA GTGCTTCTCC ATCCAAGCAT GGCAGCTCGC 
CTGGCTGCTG CCTGGTGTTT GCGCTGTGTG GCTGTGGCAT TACCTTTCCA GCTGACGCCA 
TTTCTAGACA GATGTGCAGA ACGGCTCAAC AGCTTAAAAA CCTCGCCAGA AGCTGTTAGT 
GGATACAGTT TTGCAATGGC TGCTTTGTTA GGTGGAGTTC ATCAGTGTCC TTTGGGCATT 
CCTCATGCCA AGGGAAAGAT GGTTGTTAGT ATTGCTGAAG ATCTCTTACG AACTGCTGCC 
CAAAATAGCA GGCTGTCTTT ACAGCGCACC CAAGCTGGAT GGCTCTTACT TGGAGCACTC 
ATGACTTTAG GACCGTCTGT TGTTCGTTAC CATCTACCCA AGATGTTGTT ATTGTGGCGA 
AACGTTTTCC CTCGTTCTTT AAAGGAATTG GAGGCTGAGA AGGCCCGAGG CGATTCTTTT 
ACCTGGCAGG TAACATTGGA AGGTCGTGCT GGAGCTTTAT GTGCAATGAG GAGTTTTGTT 
GCACATTGCC CTGAACTCCT AACTGAAGAT GTGATTAGAA AATTGATGAC CCCTATTGAA 
TGTGCCATGA CTATGATGTC ACACATTCCC TCTGTAATTA AAGCCCATGG AGCTCATCTG 
AAAGCTAGTG CTGCCATGGT CCGTTTAAGG CTTTATGATA TCTTGGCATT GTTACCTCCA 
AAAACTTATG AAGGCTCTTT CAATGCACTT CTTAGAGAAC TGGTGGCAGA ATTCACTTTG 
ACTGACAACT CAGCCAACAC AACTACTTCC CTCCTCAGAT CCCTCTGCCA CTATGATGAT 
AGTGTTCTAC TTGGGTCCTG GCTTCAGGAA ACTGATCATA AATCAATTGA AGACCAGCTC 
CAGCCAAACA GTGCATCTGG GAGTGGGGCT TTGGAGCATG ACCCATCCTC CATTTACTTA 
CGAATACCTG CTGGAGAAGC TGTGCCTGGG CCTCTTCCCC TTGGAGTTTC GGTCATTGAC 
GCTTCCGTGG CTCTTTTTGG TGTAGTGTTT CCTCATGTTT CTTTTAAACA CCGATTACAG 
ATGTTGGATC ACTTTGCTGA ATGTGTTAAA CAAGCCAAAG GTGTTCGCCA GCAGGCGGTG 
CAGCTTAATA TCTTTACAGC TGTACTTAGT GCACTAAAGG GCCTCGCTGA AAACAAAAGT 
ACTTTAGGAC CAGAGGAAGT TCGTAAATCT GCTCTGACAC TGGTTATGGG GGCCCTGGAC 
AATCCAAACC CCATCTTACG ATGTGCAGCA GGGGAAGCTC TTGGAAGAAT GGCTCAGGTG 
GTCGGAGAAG CAACTTTTAT TGCTAGAATG GCCCAATATA GCTTTGACAA GTTGAAGTCA 
GCTCGAGACG TTGTGTCCAG AACCGGCCAC TCATTGGCTC TGGGCTGTTT GCATCGTTAT 
GTTGGTGGTA TCGGCTCAGG ACAACATCTG AAGACCAGTG TCAGCATTTT GTTGGCCCTG 
GCACAGGATG GAACATCCTC TGAAGTCCAG ACTTGGTCCC TTCACTCGCT TGCTTTGATA 
GTGGATTCTA GTGGCCCAAT GTATCGTGGA TACGTAGAGC CAACGTTATC TCTAGTTCTT 
ACCTTGCTGT TGACAGTTCC ACCCTCACAT ACAGAAGTTC ATCAGTGTTT GGGGAGATGC 
TTGGGTGCTA TAATAACTAC TGTTGGACCG GAACTACAAG GGAATGGAGC AACAATTTCC 
ACAATCCGTT CTTCTTGTTT GGTGGGTTGT GCGATAACCC AGGACCATTC AGATTCTCTG 
GTCCAGGCAG CTGCCATATC TTGCCTTCAG CAGCTTCACA TGTTTGCACC ACGACATGTC 
AATTTATCCA GTCTTGTTCC TAGCCTTTGT GTTCACCTGT GTAGTTCCCA TCTATTACTT 
CGACGAGCAG CTGTGGCCTG TCTCCGGCAA CTTGCTCAAA GAGAAGCAGC TGAAGTATGT 
GAATACGCCA TGAGCCTGGC AAAAAGTTCA GGGGACAAAG AGAGCAGTGG TACCAATGTT 
AGTCCTTTTG CGCCCGGAGC AAGTTCTCGA AGTGATATCC ATTGCCGGCA TCAAGGTGTT 
AATATAACAG AAACTGGTCT CGAGGGACTT CTTTTTGGAA TGCTAGACCG GGAGACAGAC 
AGAAAATTAT GTTCTGATAT CCATGATACT TTGGGACATA TGCTGTCATC GCTGGCTGTA 
GAAAAGCTTT CTCATTGGCT AATGCTTTGT AAAGATGTCC TAGCAGCTTC TAGTGATATG 
AGTACTGCAA CTCCCTTAAG CAGTGGAAAA GATGAGGAAT TTGAGAAGAA AGATGAGATG 
GATGATGATA CCATGTTTAC CACACTCGGA GAAGAAGATA AATCAAAGCC CTTTGTGGCA 
CCTCGCTGGG CCACTCGAGT ATTTGCTGCT GATTGCCTGT GTCGAATCAT CAATCTGTGT 
GAGAATGCAG ACCAAGCTCA CTTTGATCTT GCCATGGCAC GTTCTGCTAA ACTACGAAAC 
CCTACAAATG ACCTCTTGGT ACTTCATCTC TCTGACCTAA TTCGCATGGC ATTCATGGCT 
GCAACCGATC ATAGTAACCA GCTGCGGATG GCTGGTCTCC AAGCCCTCGA AGACATTATC 
AAGAAGTTTG CCTCTGTGCC TGAGCCAGAA TTCCCAGGTC ATGTGATTCT GGAGCAGTAT 
CAAGCTAATG TGGGAGCTGC TCTAAGACCA GCCTTTTCAC AAGATACCCC ATCAGACATA 
ATAGCAAAAG CTTGCCAGGT GTGTAGTACA TGGATTGGAA GTGGAGTTGT CAGTGATCTC 
AATGATCTCC GTCGAGTACA CAATCTGCTT GTTTCTTCAC TGGACAAAGT TCAGGCCGGA 
AAAGGATCTT CCAGCCAACT GTACCGAGAG AGTGCTACAA CCATGGAAAA ACTGGCTGTT 
CTCAAAGCAT GGGCAGAGGT CTATGTGGTT GCTATGAATA TTAAAAAAGA AGCAGAGTCA 
AAACCAAAAA GAGCAGTTAA AAATACTGAT GACGATGATG ATGATGACAG TACCATAGAT 
GAACTACCAC CAGATAGTTT AATAACACTG GTACAGCCTG AACTGCCAAT ACTTAGTCGC 
CTGTGGTTGG CAGCATTAAA AGACTACGCG CTTTTAACTT TACCAGCTGA ATTCGCTAGT 
CAACTCCCTC CAGATGGTGG GGCATTTTAT ACTCCTGAGA CTATTGATAC GGCTAGACTG 
CACTATCGGA ATTCCTGGGC CCCAATTCTC CACGCGGTGG CACTTTGGTT AAACAGCACA 
GGATTTACAT GTTCAGAGTC AGCAGAAGCA GCAGCAATAT CCGCTTTACA AAAACGTTCC 
ATACCGGTCA ATTTAAGCCA AGCATCAGGA GCAATACCTA GTGCTAAATC TTTGGCAGAA 
ATTAACAAAG ACAGAATGCA TCTGATTTTA GGTGTGAGTA TACAGTTCCT TTGTTCCCCA 
AGACCTGAGG AACCTGTTGA ACATGTTACA GCTTGTCTAC AGGCCTTGCA CACCTTGCTA 
GATTCTCCTT ATGCCCGAAT CCATATTGCA GAAGATCAGC TGATTGGTGT TGAGTTACTG 
AGTGTTTTAC ACCGCCTCCT GTTGACCTGG AATCCACCGT CTGTCCAGCT GCTGGTGACT 
GGAGTTGTAC AACAGATAGT AAGAGCTGCT CAGGATTATT TGCAAGAAAA AAGAAACACA 
CTAAATGAGG ATGATATAGA AAAAGAGTCC TGTCCTGTAT TAGGAGAAGG AGGTGACAGT 
GGTGGTCTCA TTCCTGGAAA ATCTCTTGTG TTTGCAACTC TGGAGCTGTT GATGTTCATT 
TTAGTACGTC ATATGCCACA TCTCAGTACC AAGATGTCGG ACTCTCCAAG TCACATAGCC 
ACTAAAACTC GACTGTCGGA AGAAAGTGCT CGTTTGGTGG CAGCCACAGT TACCATACTC 
TCCGACTTAC CGTCTCTCTG TTCACCTGCT GGATGTATGA CAATCTTGCC CACAATTCTG 
TTTTTAATTG CAAGAATATT GAAAGACACA GCAATAAAGT CTGCAGATAA TCAGGTTCCT 
CCACCAGTCA GTGCAGCTCT TCAAGGGATC AAAAGTATTG TGACACTTTC AATGGCCAAA 
ACTGAGGATA CTCAAAAACA GTGGACAGCT CTAATTCGTA GCACTCTGGC ATGCATCCTA 
GAATATTCCC AACCAGAAGA TTCTGTGCCT GTGCCTGATG AAGTAAGCAT GCTAACAGCA 
ATTGCACTTT TCCTGTGGTC TGCTGGTAGT GAAATAATAG GAGTTCAGTC ACTGCAGAAT 
GGCTGCATTT ACAGATTTAA AAATGCGTTA AATTCATGTG ACCCGTGGGT TCAAGCCAAA 
TGCTACCAGC TTCTCCTCTC AGTTTTTCAA CATTCAAATC GGGCCCTTTC AACTCCTTAT 
ATCCATTCCT TAGCTCCAAT AGTAGTTGAA AAGCTAAAAG CTGTTGAAAG AAACAGACCA 
GGCAGTAGTA CAGAACTCTT AGCTGTTCAA GAAGGAATCA AAGTTCTTGA AACATTGGTT 
GCTCTTGGGG AAGAACAGAA CAGAGTCCAA TTACTTGCTC TTTTAGTTCC CACTTTGATC 
TCTTACCTGC TGGATGAAAA TTCTTTTGCC TCAGCAAGCA CAGCTTCCAA AGATCTCCAT 
GAGTTTGCAC TCCAGAATTT AATGCATATT GGACCTCTGT ATCCACACGC TTTCAAGACA 
GTAATGGGAG CAGCTCCTGA GCTGAAAGTA CGTCTGGAAA CTGCTGTTAG AGCAAGTCAA 
GCTAGTAAGG CTAAAGTGGC CACCAGGCAG CCAGCCCCAA CCGTGCACTC TGCACCAACA 
ATTAAGCTAA AAACAAGTTT CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855141.1
CDS	75..5741
Translation	MYFLLTAGISREYAGTTCLLELQNEAVFMWTAELENIATLCFKALENSNYGVRVAVSKLLGTVMATALMPKQATVMRQNVKRATFDEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSGENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNSLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSFKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSSEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKSSGDKESSGTNVSPFAPGASSRSDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVEKLSHWLMLCKDVLAASSDMSTATPLSSGKDEEFEKKDEMDDDTMFTTLGEEDKSKPFVAPRWATRVFAADCLCRIINLCENADQAHFDLAMARSAKLRNPTNDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAESKPKRAVKNTDDDDDDDSTIDELPPDSLITLVQPELPILSRLWLAALKDYALLTLPAEFASQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTCSESAEAAAISALQKRSIPVNLSQASGAIPSAKSLAEINKDRMHLILGVSIQFLCSPRPEEPVEHVTACLQALHTLLDSPYARIHIAEDQLIGVELLSVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDIEKESCPVLGEGGDSGGLIPGKSLVFATLELLMFILVRHMPHLSTKMSDSPSHIATKTRLSEESARLVAATVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSADNQVPPPVSAALQGIKSIVTLSMAKTEDTQKQWTALIRSTLACILEYSQPEDSVPVPDEVSMLTAIALFLWSAGSEIIGVQSLQNGCIYRFKNALNSCDPWVQAKCYQLLLSVFQHSNRALSTPYIHSLAPIVVEKLKAVERNRPGSSTELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENSFASASTASKDLHEFALQNLMHIGPLYPHAFKTVMGAAPELKVRLETAVRASQASKAKVATRQPAPTVHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_024999373.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999373.1

ORF Insert Sequence:

ATGTATTTCC TCCTCACTGC TGGAATCTCA AGGGAGTATG CTGGGACAAC TTGTCTCCTA 
GAACTACAGA ATGAAGCTGT GTTCATGTGG ACTGCTGAAC TGGAAAATAT AGCCACTCTC 
TGCTTCAAGG CTTTGGAAAA CTCTAACTAT GGGGTACGAG TGGCAGTGTC TAAGCTCTTA 
GGAACAGTCA TGGCTACAGC ACTGATGCCA AAACAAGCAA CAGTAATGCG CCAGAATGTG 
AAGCGAGCAA CCTTTGATGA AGTCTTAGAA CTCATGGCCA CAGGATTTCT GCGTGGAGGG 
TCAGGTTTCT TAAAGAGTGG TGGAGAAATG TTAAAAGTTG GAGGGTCTGT TAATCGTGAA 
GTGAGAGTTG GAGTTACCCA GGCATATGTT GTTTTTGTAA CAACATTGGG TGGCCAGTGG 
TTGGAGCGTA GTTTTGCTAC ATTCTTGTCT CATGTACTTG ATCTGGTTTC CCATCCTCGG 
GCAACACAAA CACATGTGGA GGCTGTGTAT TCCAGACGAT GTGTCTCCTT TATTTTAAGA 
GCTACCGTGG GCAGTTTGCT AGGTGAAAAA GCCCAGATTG CAGCTGCCAA AGAAATATGC 
CAGGCGATTG GAAAACAAAT GAAAGCAGTA GAAGCGGTAG TGAATGACAC TAGTGGGGAG 
AACAAGTCTG GAGCAGCAGA CATTGCAGCA AGCCAGCATG TGATGGTCTG TGCCCTGCAG 
GAGCTCGGGA GCCTGGTGCA GAGCCTGAAT GCCACCGCAT CCCCTCTTAT TCAGGAAGCA 
TCTATAGGCC TTTTGGAGAC TGTGACCTCA GTGCTTCTCC ATCCAAGCAT GGCAGCTCGC 
CTGGCTGCTG CCTGGTGTTT GCGCTGTGTG GCTGTGGCAT TACCTTTCCA GCTGACGCCA 
TTTCTAGACA GATGTGCAGA ACGGCTCAAC AGCTTAAAAA CCTCGCCAGA AGCTGTTAGT 
GGATACAGTT TTGCAATGGC TGCTTTGTTA GGTGGAGTTC ATCAGTGTCC TTTGGGCATT 
CCTCATGCCA AGGGAAAGAT GGTTGTTAGT ATTGCTGAAG ATCTCTTACG AACTGCTGCC 
CAAAATAGCA GGCTGTCTTT ACAGCGCACC CAAGCTGGAT GGCTCTTACT TGGAGCACTC 
ATGACTTTAG GACCGTCTGT TGTTCGTTAC CATCTACCCA AGATGTTGTT ATTGTGGCGA 
AACGTTTTCC CTCGTTCTTT AAAGGAATTG GAGGCTGAGA AGGCCCGAGG CGATTCTTTT 
ACCTGGCAGG TAACATTGGA AGGTCGTGCT GGAGCTTTAT GTGCAATGAG GAGTTTTGTT 
GCACATTGCC CTGAACTCCT AACTGAAGAT GTGATTAGAA AATTGATGAC CCCTATTGAA 
TGTGCCATGA CTATGATGTC ACACATTCCC TCTGTAATTA AAGCCCATGG AGCTCATCTG 
AAAGCTAGTG CTGCCATGGT CCGTTTAAGG CTTTATGATA TCTTGGCATT GTTACCTCCA 
AAAACTTATG AAGGCTCTTT CAATGCACTT CTTAGAGAAC TGGTGGCAGA ATTCACTTTG 
ACTGACAACT CAGCCAACAC AACTACTTCC CTCCTCAGAT CCCTCTGCCA CTATGATGAT 
AGTGTTCTAC TTGGGTCCTG GCTTCAGGAA ACTGATCATA AATCAATTGA AGACCAGCTC 
CAGCCAAACA GTGCATCTGG GAGTGGGGCT TTGGAGCATG ACCCATCCTC CATTTACTTA 
CGAATACCTG CTGGAGAAGC TGTGCCTGGG CCTCTTCCCC TTGGAGTTTC GGTCATTGAC 
GCTTCCGTGG CTCTTTTTGG TGTAGTGTTT CCTCATGTTT CTTTTAAACA CCGATTACAG 
ATGTTGGATC ACTTTGCTGA ATGTGTTAAA CAAGCCAAAG GTGTTCGCCA GCAGGCGGTG 
CAGCTTAATA TCTTTACAGC TGTACTTAGT GCACTAAAGG GCCTCGCTGA AAACAAAAGT 
ACTTTAGGAC CAGAGGAAGT TCGTAAATCT GCTCTGACAC TGGTTATGGG GGCCCTGGAC 
AATCCAAACC CCATCTTACG ATGTGCAGCA GGGGAAGCTC TTGGAAGAAT GGCTCAGGTG 
GTCGGAGAAG CAACTTTTAT TGCTAGAATG GCCCAATATA GCTTTGACAA GTTGAAGTCA 
GCTCGAGACG TTGTGTCCAG AACCGGCCAC TCATTGGCTC TGGGCTGTTT GCATCGTTAT 
GTTGGTGGTA TCGGCTCAGG ACAACATCTG AAGACCAGTG TCAGCATTTT GTTGGCCCTG 
GCACAGGATG GAACATCCTC TGAAGTCCAG ACTTGGTCCC TTCACTCGCT TGCTTTGATA 
GTGGATTCTA GTGGCCCAAT GTATCGTGGA TACGTAGAGC CAACGTTATC TCTAGTTCTT 
ACCTTGCTGT TGACAGTTCC ACCCTCACAT ACAGAAGTTC ATCAGTGTTT GGGGAGATGC 
TTGGGTGCTA TAATAACTAC TGTTGGACCG GAACTACAAG GGAATGGAGC AACAATTTCC 
ACAATCCGTT CTTCTTGTTT GGTGGGTTGT GCGATAACCC AGGACCATTC AGATTCTCTG 
GTCCAGGCAG CTGCCATATC TTGCCTTCAG CAGCTTCACA TGTTTGCACC ACGACATGTC 
AATTTATCCA GTCTTGTTCC TAGCCTTTGT GTTCACCTGT GTAGTTCCCA TCTATTACTT 
CGACGAGCAG CTGTGGCCTG TCTCCGGCAA CTTGCTCAAA GAGAAGCAGC TGAAGTATGT 
GAATACGCCA TGAGCCTGGC AAAAAGTTCA GGGGACAAAG AGAGCAGTGG TACCAATGTT 
AGTCCTTTTG CGCCCGGAGC AAGTTCTCGA AGTGATATCC ATTGCCGGCA TCAAGGTGTT 
AATATAACAG AAACTGGTCT CGAGGGACTT CTTTTTGGAA TGCTAGACCG GGAGACAGAC 
AGAAAATTAT GTTCTGATAT CCATGATACT TTGGGACATA TGCTGTCATC GCTGGCTGTA 
GAAAAGCTTT CTCATTGGCT AATGCTTTGT AAAGATGTCC TAGCAGCTTC TAGTGATATG 
AGTACTGCAA CTCCCTTAAG CAGTGGAAAA GATGAGGAAT TTGAGAAGAA AGATGAGATG 
GATGATGATA CCATGTTTAC CACACTCGGA GAAGAAGATA AATCAAAGCC CTTTGTGGCA 
CCTCGCTGGG CCACTCGAGT ATTTGCTGCT GATTGCCTGT GTCGAATCAT CAATCTGTGT 
GAGAATGCAG ACCAAGCTCA CTTTGATCTT GCCATGGCAC GTTCTGCTAA ACTACGAAAC 
CCTACAAATG ACCTCTTGGT ACTTCATCTC TCTGACCTAA TTCGCATGGC ATTCATGGCT 
GCAACCGATC ATAGTAACCA GCTGCGGATG GCTGGTCTCC AAGCCCTCGA AGACATTATC 
AAGAAGTTTG CCTCTGTGCC TGAGCCAGAA TTCCCAGGTC ATGTGATTCT GGAGCAGTAT 
CAAGCTAATG TGGGAGCTGC TCTAAGACCA GCCTTTTCAC AAGATACCCC ATCAGACATA 
ATAGCAAAAG CTTGCCAGGT GTGTAGTACA TGGATTGGAA GTGGAGTTGT CAGTGATCTC 
AATGATCTCC GTCGAGTACA CAATCTGCTT GTTTCTTCAC TGGACAAAGT TCAGGCCGGA 
AAAGGATCTT CCAGCCAACT GTACCGAGAG AGTGCTACAA CCATGGAAAA ACTGGCTGTT 
CTCAAAGCAT GGGCAGAGGT CTATGTGGTT GCTATGAATA TTAAAAAAGA AGCAGAGTCA 
AAACCAAAAA GAGCAGTTAA AAATACTGAT GACGATGATG ATGATGACAG TACCATAGAT 
GAACTACCAC CAGATAGTTT AATAACACTG GTACAGCCTG AACTGCCAAT ACTTAGTCGC 
CTGTGGTTGG CAGCATTAAA AGACTACGCG CTTTTAACTT TACCAGCTGA ATTCGCTAGT 
CAACTCCCTC CAGATGGTGG GGCATTTTAT ACTCCTGAGA CTATTGATAC GGCTAGACTG 
CACTATCGGA ATTCCTGGGC CCCAATTCTC CACGCGGTGG CACTTTGGTT AAACAGCACA 
GGATTTACAT GTTCAGAGTC AGCAGAAGCA GCAGCAATAT CCGCTTTACA AAAACGTTCC 
ATACCGGTCA ATTTAAGCCA AGCATCAGGA GCAATACCTA GTGCTAAATC TTTGGCAGAA 
ATTAACAAAG ACAGAATGCA TCTGATTTTA GGTGTGAGTA TACAGTTCCT TTGTTCCCCA 
AGACCTGAGG AACCTGTTGA ACATGTTACA GCTTGTCTAC AGGCCTTGCA CACCTTGCTA 
GATTCTCCTT ATGCCCGAAT CCATATTGCA GAAGATCAGC TGATTGGTGT TGAGTTACTG 
AGTGTTTTAC ACCGCCTCCT GTTGACCTGG AATCCACCGT CTGTCCAGCT GCTGGTGACT 
GGAGTTGTAC AACAGATAGT AAGAGCTGCT CAGGATTATT TGCAAGAAAA AAGAAACACA 
CTAAATGAGG ATGATATAGA AAAAGAGTCC TGTCCTGTAT TAGGAGAAGG AGGTGACAGT 
GGTGGTCTCA TTCCTGGAAA ATCTCTTGTG TTTGCAACTC TGGAGCTGTT GATGTTCATT 
TTAGTACGTC ATATGCCACA TCTCAGTACC AAGATGTCGG ACTCTCCAAG TCACATAGCC 
ACTAAAACTC GACTGTCGGA AGAAAGTGCT CGTTTGGTGG CAGCCACAGT TACCATACTC 
TCCGACTTAC CGTCTCTCTG TTCACCTGCT GGATGTATGA CAATCTTGCC CACAATTCTG 
TTTTTAATTG CAAGAATATT GAAAGACACA GCAATAAAGT CTGCAGATAA TCAGGTTCCT 
CCACCAGTCA GTGCAGCTCT TCAAGGGATC AAAAGTATTG TGACACTTTC AATGGCCAAA 
ACTGAGGATA CTCAAAAACA GTGGACAGCT CTAATTCGTA GCACTCTGGC ATGCATCCTA 
GAATATTCCC AACCAGAAGA TTCTGTGCCT GTGCCTGATG AAGTAAGCAT GCTAACAGCA 
ATTGCACTTT TCCTGTGGTC TGCTGGTAGT GAAATAATAG GAGTTCAGTC ACTGCAGAAT 
GGCTGCATTT ACAGATTTAA AAATGCGTTA AATTCATGTG ACCCGTGGGT TCAAGCCAAA 
TGCTACCAGC TTCTCCTCTC AGTTTTTCAA CATTCAAATC GGGCCCTTTC AACTCCTTAT 
ATCCATTCCT TAGCTCCAAT AGTAGTTGAA AAGCTAAAAG CTGTTGAAAG AAACAGACCA 
GGCAGTAGTA CAGAACTCTT AGCTGTTCAA GAAGGAATCA AAGTTCTTGA AACATTGGTT 
GCTCTTGGGG AAGAACAGAA CAGAGTCCAA TTACTTGCTC TTTTAGTTCC CACTTTGATC 
TCTTACCTGC TGGATGAAAA TTCTTTTGCC TCAGCAAGCA CAGCTTCCAA AGATCTCCAT 
GAGTTTGCAC TCCAGAATTT AATGCATATT GGACCTCTGT ATCCACACGC TTTCAAGACA 
GTAATGGGAG CAGCTCCTGA GCTGAAAGTA CGTCTGGAAA CTGCTGTTAG AGCAAGTCAA 
GCTAGTAAGG CTAAAGTGGC CACCAGGCAG CCAGCCCCAA CCGTGCACTC TGCACCAACA 
ATTAAGCTAA AAACAAGTTT CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_024999374.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855142.1 Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X5, mRNA.

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CloneID	OBa220803
Clone ID Related Accession (Same CDS sequence)	XM_024999374.1
Accession Version	XM_024999374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	HEAT repeat-containing protein 5B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGTTATAAAA ATTGTCTCAG TGACCAACCT TATAAATTCC 
TTATGCACAT GTGCTATGAG ATTTTCTACA GCATACCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855142.1
CDS	134..5755
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKKLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIRNKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKSAESQGRSEILMSLQKVLNGLGGAAASCHRDIYKNARSLLTDRSMAVRCAVAKCLLELQNEAVFMWTAELENIATLCFKALENSNYGVRVAVSKLLGTVMATALMPKQATVMRQNVKRATFDEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSGENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNSLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSFKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSSEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKSSGDKESSGTNVSPFAPGASSRSDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVEKLSHWLMLCKDVLAASSDMSTATPLSSGKDEEFEKKDEMDDDTMFTTLGEEDKSKPFVAPRWATRVFAADCLCRIINLCENADQAHFDLAMARSAKLRNPTNDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAESKPKRAVKNTDDDDDDDSTIDELPPDSLITLVQPELPILSRLWLAALKDYALLTLPAEFASQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTCSESAEAAAISALQKRSIPVNLSQASGAIPSAKSLAEINKDRMHLILGVSIQFLCSPRPEEPVEHVTACLQALHTLLDSPYARIHIAEDQLIGVELLSVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDIEKESCPVLGEGGDSGGLIPGKSLVFATLELLMFILVRHMPHLSTKMSDSPSHIATKTRLSEESARLVAATVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSADNQVPPPVSAALQGIKSIVTLSMAKTEDTQKQWTALIRSTLACILEYSQPVIKIVSVTNLINSLCTCAMRFSTAYP

Target ORF information:

RefSeq Version	XM_024999374.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999374.1

ORF Insert Sequence:

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGTTATAAAA ATTGTCTCAG TGACCAACCT TATAAATTCC 
TTATGCACAT GTGCTATGAG ATTTTCTACA GCATACCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_002691283.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002691329.1 Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X1, mRNA.

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CloneID	OBa23993
Clone ID Related Accession (Same CDS sequence)	XM_002691283.5 , XM_005212706.3 , XM_002691283.6 , XM_005212706.4 , XM_015465330.1 , XM_015465331.1
Accession Version	XM_002691283.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	HEAT repeat-containing protein 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002691283.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGAAGATTCT GTGCCTGTGC CTGATGAAGT AAGCATGCTA 
ACAGCAATTG CACTTTTCCT GTGGTCTGCT GGTAGTGAAA TAATAGGAGT TCAGTCACTG 
CAGAATGGCT GCATTTACAG ATTTAAAAAT GCGTTAAATT CATGTGACCC GTGGGTTCAA 
GCCAAATGCT ACCAGCTTCT CCTCTCAGTT TTTCAACATT CAAATCGGGC CCTTTCAACT 
CCTTATATCC ATTCCTTAGC TCCAATAGTA GTTGAAAAGC TAAAAGCTGT TGAAAGAAAC 
AGACCAGGCA GTAGTACAGA ACTCTTAGCT GTTCAAGAAG GAATCAAAGT TCTTGAAACA 
TTGGTTGCTC TTGGGGAAGA ACAGAACAGA GTCCAATTAC TTGCTCTTTT AGTTCCCACT 
TTGATCTCTT ACCTGCTGGA TGAAAATTCT TTTGCCTCAG CAAGCACAGC TTCCAAAGAT 
CTCCATGAGT TTGCACTCCA GAATTTAATG CATATTGGAC CTCTGTATCC ACACGCTTTC 
AAGACAGTAA TGGGAGCAGC TCCTGAGCTG AAAGTACGTC TGGAAACTGC TGTTAGAGCA 
AGTCAAGCTA GTAAGGCTAA AGTGGCCACC AGGCAGCCAG CCCCAACCGT GCACTCTGCA 
CCAACAATTA AGCTAAAAAC AAGTTTCTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002691329.1
CDS	109..6321
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKKLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIRNKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKSAESQGRSEILMSLQKVLNGLGGAAASCHRDIYKNARSLLTDRSMAVRCAVAKCLLELQNEAVFMWTAELENIATLCFKALENSNYGVRVAVSKLLGTVMATALMPKQATVMRQNVKRATFDEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSGENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNSLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSFKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSSEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKSSGDKESSGTNVSPFAPGASSRSDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVEKLSHWLMLCKDVLAASSDMSTATPLSSGKDEEFEKKDEMDDDTMFTTLGEEDKSKPFVAPRWATRVFAADCLCRIINLCENADQAHFDLAMARSAKLRNPTNDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAESKPKRAVKNTDDDDDDDSTIDELPPDSLITLVQPELPILSRLWLAALKDYALLTLPAEFASQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTCSESAEAAAISALQKRSIPVNLSQASGAIPSAKSLAEINKDRMHLILGVSIQFLCSPRPEEPVEHVTACLQALHTLLDSPYARIHIAEDQLIGVELLSVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDIEKESCPVLGEGGDSGGLIPGKSLVFATLELLMFILVRHMPHLSTKMSDSPSHIATKTRLSEESARLVAATVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSADNQVPPPVSAALQGIKSIVTLSMAKTEDTQKQWTALIRSTLACILEYSQPEDSVPVPDEVSMLTAIALFLWSAGSEIIGVQSLQNGCIYRFKNALNSCDPWVQAKCYQLLLSVFQHSNRALSTPYIHSLAPIVVEKLKAVERNRPGSSTELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENSFASASTASKDLHEFALQNLMHIGPLYPHAFKTVMGAAPELKVRLETAVRASQASKAKVATRQPAPTVHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_002691283.5
Organism	Bos taurus(cattle)
Definition	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002691283.5

ORF Insert Sequence:

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGAAGATTCT GTGCCTGTGC CTGATGAAGT AAGCATGCTA 
ACAGCAATTG CACTTTTCCT GTGGTCTGCT GGTAGTGAAA TAATAGGAGT TCAGTCACTG 
CAGAATGGCT GCATTTACAG ATTTAAAAAT GCGTTAAATT CATGTGACCC GTGGGTTCAA 
GCCAAATGCT ACCAGCTTCT CCTCTCAGTT TTTCAACATT CAAATCGGGC CCTTTCAACT 
CCTTATATCC ATTCCTTAGC TCCAATAGTA GTTGAAAAGC TAAAAGCTGT TGAAAGAAAC 
AGACCAGGCA GTAGTACAGA ACTCTTAGCT GTTCAAGAAG GAATCAAAGT TCTTGAAACA 
TTGGTTGCTC TTGGGGAAGA ACAGAACAGA GTCCAATTAC TTGCTCTTTT AGTTCCCACT 
TTGATCTCTT ACCTGCTGGA TGAAAATTCT TTTGCCTCAG CAAGCACAGC TTCCAAAGAT 
CTCCATGAGT TTGCACTCCA GAATTTAATG CATATTGGAC CTCTGTATCC ACACGCTTTC 
AAGACAGTAA TGGGAGCAGC TCCTGAGCTG AAAGTACGTC TGGAAACTGC TGTTAGAGCA 
AGTCAAGCTA GTAAGGCTAA AGTGGCCACC AGGCAGCCAG CCCCAACCGT GCACTCTGCA 
CCAACAATTA AGCTAAAAAC AAGTTTCTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_005212706.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212763.1 Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X2, mRNA.

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CloneID	OBa23993
Clone ID Related Accession (Same CDS sequence)	XM_002691283.5 , XM_005212706.3 , XM_002691283.6 , XM_005212706.4 , XM_015465330.1 , XM_015465331.1
Accession Version	XM_005212706.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	HEAT repeat-containing protein 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGAAGATTCT GTGCCTGTGC CTGATGAAGT AAGCATGCTA 
ACAGCAATTG CACTTTTCCT GTGGTCTGCT GGTAGTGAAA TAATAGGAGT TCAGTCACTG 
CAGAATGGCT GCATTTACAG ATTTAAAAAT GCGTTAAATT CATGTGACCC GTGGGTTCAA 
GCCAAATGCT ACCAGCTTCT CCTCTCAGTT TTTCAACATT CAAATCGGGC CCTTTCAACT 
CCTTATATCC ATTCCTTAGC TCCAATAGTA GTTGAAAAGC TAAAAGCTGT TGAAAGAAAC 
AGACCAGGCA GTAGTACAGA ACTCTTAGCT GTTCAAGAAG GAATCAAAGT TCTTGAAACA 
TTGGTTGCTC TTGGGGAAGA ACAGAACAGA GTCCAATTAC TTGCTCTTTT AGTTCCCACT 
TTGATCTCTT ACCTGCTGGA TGAAAATTCT TTTGCCTCAG CAAGCACAGC TTCCAAAGAT 
CTCCATGAGT TTGCACTCCA GAATTTAATG CATATTGGAC CTCTGTATCC ACACGCTTTC 
AAGACAGTAA TGGGAGCAGC TCCTGAGCTG AAAGTACGTC TGGAAACTGC TGTTAGAGCA 
AGTCAAGCTA GTAAGGCTAA AGTGGCCACC AGGCAGCCAG CCCCAACCGT GCACTCTGCA 
CCAACAATTA AGCTAAAAAC AAGTTTCTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212763.1
CDS	188..6400
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKKLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIRNKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKSAESQGRSEILMSLQKVLNGLGGAAASCHRDIYKNARSLLTDRSMAVRCAVAKCLLELQNEAVFMWTAELENIATLCFKALENSNYGVRVAVSKLLGTVMATALMPKQATVMRQNVKRATFDEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSGENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNSLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSFKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSSEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKSSGDKESSGTNVSPFAPGASSRSDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVEKLSHWLMLCKDVLAASSDMSTATPLSSGKDEEFEKKDEMDDDTMFTTLGEEDKSKPFVAPRWATRVFAADCLCRIINLCENADQAHFDLAMARSAKLRNPTNDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAESKPKRAVKNTDDDDDDDSTIDELPPDSLITLVQPELPILSRLWLAALKDYALLTLPAEFASQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTCSESAEAAAISALQKRSIPVNLSQASGAIPSAKSLAEINKDRMHLILGVSIQFLCSPRPEEPVEHVTACLQALHTLLDSPYARIHIAEDQLIGVELLSVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDIEKESCPVLGEGGDSGGLIPGKSLVFATLELLMFILVRHMPHLSTKMSDSPSHIATKTRLSEESARLVAATVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSADNQVPPPVSAALQGIKSIVTLSMAKTEDTQKQWTALIRSTLACILEYSQPEDSVPVPDEVSMLTAIALFLWSAGSEIIGVQSLQNGCIYRFKNALNSCDPWVQAKCYQLLLSVFQHSNRALSTPYIHSLAPIVVEKLKAVERNRPGSSTELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENSFASASTASKDLHEFALQNLMHIGPLYPHAFKTVMGAAPELKVRLETAVRASQASKAKVATRQPAPTVHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_005212706.3
Organism	Bos taurus(cattle)
Definition	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212706.3

ORF Insert Sequence:

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGAAGATTCT GTGCCTGTGC CTGATGAAGT AAGCATGCTA 
ACAGCAATTG CACTTTTCCT GTGGTCTGCT GGTAGTGAAA TAATAGGAGT TCAGTCACTG 
CAGAATGGCT GCATTTACAG ATTTAAAAAT GCGTTAAATT CATGTGACCC GTGGGTTCAA 
GCCAAATGCT ACCAGCTTCT CCTCTCAGTT TTTCAACATT CAAATCGGGC CCTTTCAACT 
CCTTATATCC ATTCCTTAGC TCCAATAGTA GTTGAAAAGC TAAAAGCTGT TGAAAGAAAC 
AGACCAGGCA GTAGTACAGA ACTCTTAGCT GTTCAAGAAG GAATCAAAGT TCTTGAAACA 
TTGGTTGCTC TTGGGGAAGA ACAGAACAGA GTCCAATTAC TTGCTCTTTT AGTTCCCACT 
TTGATCTCTT ACCTGCTGGA TGAAAATTCT TTTGCCTCAG CAAGCACAGC TTCCAAAGAT 
CTCCATGAGT TTGCACTCCA GAATTTAATG CATATTGGAC CTCTGTATCC ACACGCTTTC 
AAGACAGTAA TGGGAGCAGC TCCTGAGCTG AAAGTACGTC TGGAAACTGC TGTTAGAGCA 
AGTCAAGCTA GTAAGGCTAA AGTGGCCACC AGGCAGCCAG CCCCAACCGT GCACTCTGCA 
CCAACAATTA AGCTAAAAAC AAGTTTCTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_002691283.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002691329.1 Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X1, mRNA.

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CloneID	OBa23993
Clone ID Related Accession (Same CDS sequence)	XM_002691283.5 , XM_005212706.3 , XM_002691283.6 , XM_005212706.4 , XM_015465330.1 , XM_015465331.1
Accession Version	XM_002691283.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	HEAT repeat-containing protein 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002691283.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGAAGATTCT GTGCCTGTGC CTGATGAAGT AAGCATGCTA 
ACAGCAATTG CACTTTTCCT GTGGTCTGCT GGTAGTGAAA TAATAGGAGT TCAGTCACTG 
CAGAATGGCT GCATTTACAG ATTTAAAAAT GCGTTAAATT CATGTGACCC GTGGGTTCAA 
GCCAAATGCT ACCAGCTTCT CCTCTCAGTT TTTCAACATT CAAATCGGGC CCTTTCAACT 
CCTTATATCC ATTCCTTAGC TCCAATAGTA GTTGAAAAGC TAAAAGCTGT TGAAAGAAAC 
AGACCAGGCA GTAGTACAGA ACTCTTAGCT GTTCAAGAAG GAATCAAAGT TCTTGAAACA 
TTGGTTGCTC TTGGGGAAGA ACAGAACAGA GTCCAATTAC TTGCTCTTTT AGTTCCCACT 
TTGATCTCTT ACCTGCTGGA TGAAAATTCT TTTGCCTCAG CAAGCACAGC TTCCAAAGAT 
CTCCATGAGT TTGCACTCCA GAATTTAATG CATATTGGAC CTCTGTATCC ACACGCTTTC 
AAGACAGTAA TGGGAGCAGC TCCTGAGCTG AAAGTACGTC TGGAAACTGC TGTTAGAGCA 
AGTCAAGCTA GTAAGGCTAA AGTGGCCACC AGGCAGCCAG CCCCAACCGT GCACTCTGCA 
CCAACAATTA AGCTAAAAAC AAGTTTCTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002691329.1
CDS	133..6345
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKKLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIRNKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKSAESQGRSEILMSLQKVLNGLGGAAASCHRDIYKNARSLLTDRSMAVRCAVAKCLLELQNEAVFMWTAELENIATLCFKALENSNYGVRVAVSKLLGTVMATALMPKQATVMRQNVKRATFDEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSGENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNSLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSFKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSSEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKSSGDKESSGTNVSPFAPGASSRSDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVEKLSHWLMLCKDVLAASSDMSTATPLSSGKDEEFEKKDEMDDDTMFTTLGEEDKSKPFVAPRWATRVFAADCLCRIINLCENADQAHFDLAMARSAKLRNPTNDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAESKPKRAVKNTDDDDDDDSTIDELPPDSLITLVQPELPILSRLWLAALKDYALLTLPAEFASQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTCSESAEAAAISALQKRSIPVNLSQASGAIPSAKSLAEINKDRMHLILGVSIQFLCSPRPEEPVEHVTACLQALHTLLDSPYARIHIAEDQLIGVELLSVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDIEKESCPVLGEGGDSGGLIPGKSLVFATLELLMFILVRHMPHLSTKMSDSPSHIATKTRLSEESARLVAATVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSADNQVPPPVSAALQGIKSIVTLSMAKTEDTQKQWTALIRSTLACILEYSQPEDSVPVPDEVSMLTAIALFLWSAGSEIIGVQSLQNGCIYRFKNALNSCDPWVQAKCYQLLLSVFQHSNRALSTPYIHSLAPIVVEKLKAVERNRPGSSTELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENSFASASTASKDLHEFALQNLMHIGPLYPHAFKTVMGAAPELKVRLETAVRASQASKAKVATRQPAPTVHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_002691283.6
Organism	Bos taurus(cattle)
Definition	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002691283.6

ORF Insert Sequence:

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGAAGATTCT GTGCCTGTGC CTGATGAAGT AAGCATGCTA 
ACAGCAATTG CACTTTTCCT GTGGTCTGCT GGTAGTGAAA TAATAGGAGT TCAGTCACTG 
CAGAATGGCT GCATTTACAG ATTTAAAAAT GCGTTAAATT CATGTGACCC GTGGGTTCAA 
GCCAAATGCT ACCAGCTTCT CCTCTCAGTT TTTCAACATT CAAATCGGGC CCTTTCAACT 
CCTTATATCC ATTCCTTAGC TCCAATAGTA GTTGAAAAGC TAAAAGCTGT TGAAAGAAAC 
AGACCAGGCA GTAGTACAGA ACTCTTAGCT GTTCAAGAAG GAATCAAAGT TCTTGAAACA 
TTGGTTGCTC TTGGGGAAGA ACAGAACAGA GTCCAATTAC TTGCTCTTTT AGTTCCCACT 
TTGATCTCTT ACCTGCTGGA TGAAAATTCT TTTGCCTCAG CAAGCACAGC TTCCAAAGAT 
CTCCATGAGT TTGCACTCCA GAATTTAATG CATATTGGAC CTCTGTATCC ACACGCTTTC 
AAGACAGTAA TGGGAGCAGC TCCTGAGCTG AAAGTACGTC TGGAAACTGC TGTTAGAGCA 
AGTCAAGCTA GTAAGGCTAA AGTGGCCACC AGGCAGCCAG CCCCAACCGT GCACTCTGCA 
CCAACAATTA AGCTAAAAAC AAGTTTCTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_005212706.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212763.1 Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X2, mRNA.

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CloneID	OBa23993
Clone ID Related Accession (Same CDS sequence)	XM_002691283.5 , XM_005212706.3 , XM_002691283.6 , XM_005212706.4 , XM_015465330.1 , XM_015465331.1
Accession Version	XM_005212706.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	HEAT repeat-containing protein 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212706.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGAAGATTCT GTGCCTGTGC CTGATGAAGT AAGCATGCTA 
ACAGCAATTG CACTTTTCCT GTGGTCTGCT GGTAGTGAAA TAATAGGAGT TCAGTCACTG 
CAGAATGGCT GCATTTACAG ATTTAAAAAT GCGTTAAATT CATGTGACCC GTGGGTTCAA 
GCCAAATGCT ACCAGCTTCT CCTCTCAGTT TTTCAACATT CAAATCGGGC CCTTTCAACT 
CCTTATATCC ATTCCTTAGC TCCAATAGTA GTTGAAAAGC TAAAAGCTGT TGAAAGAAAC 
AGACCAGGCA GTAGTACAGA ACTCTTAGCT GTTCAAGAAG GAATCAAAGT TCTTGAAACA 
TTGGTTGCTC TTGGGGAAGA ACAGAACAGA GTCCAATTAC TTGCTCTTTT AGTTCCCACT 
TTGATCTCTT ACCTGCTGGA TGAAAATTCT TTTGCCTCAG CAAGCACAGC TTCCAAAGAT 
CTCCATGAGT TTGCACTCCA GAATTTAATG CATATTGGAC CTCTGTATCC ACACGCTTTC 
AAGACAGTAA TGGGAGCAGC TCCTGAGCTG AAAGTACGTC TGGAAACTGC TGTTAGAGCA 
AGTCAAGCTA GTAAGGCTAA AGTGGCCACC AGGCAGCCAG CCCCAACCGT GCACTCTGCA 
CCAACAATTA AGCTAAAAAC AAGTTTCTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212763.1
CDS	211..6423
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKKLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIRNKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKSAESQGRSEILMSLQKVLNGLGGAAASCHRDIYKNARSLLTDRSMAVRCAVAKCLLELQNEAVFMWTAELENIATLCFKALENSNYGVRVAVSKLLGTVMATALMPKQATVMRQNVKRATFDEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSGENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNSLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSFKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSSEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKSSGDKESSGTNVSPFAPGASSRSDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVEKLSHWLMLCKDVLAASSDMSTATPLSSGKDEEFEKKDEMDDDTMFTTLGEEDKSKPFVAPRWATRVFAADCLCRIINLCENADQAHFDLAMARSAKLRNPTNDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAESKPKRAVKNTDDDDDDDSTIDELPPDSLITLVQPELPILSRLWLAALKDYALLTLPAEFASQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTCSESAEAAAISALQKRSIPVNLSQASGAIPSAKSLAEINKDRMHLILGVSIQFLCSPRPEEPVEHVTACLQALHTLLDSPYARIHIAEDQLIGVELLSVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDIEKESCPVLGEGGDSGGLIPGKSLVFATLELLMFILVRHMPHLSTKMSDSPSHIATKTRLSEESARLVAATVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSADNQVPPPVSAALQGIKSIVTLSMAKTEDTQKQWTALIRSTLACILEYSQPEDSVPVPDEVSMLTAIALFLWSAGSEIIGVQSLQNGCIYRFKNALNSCDPWVQAKCYQLLLSVFQHSNRALSTPYIHSLAPIVVEKLKAVERNRPGSSTELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENSFASASTASKDLHEFALQNLMHIGPLYPHAFKTVMGAAPELKVRLETAVRASQASKAKVATRQPAPTVHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_005212706.4
Organism	Bos taurus(cattle)
Definition	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212706.4

ORF Insert Sequence:

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGAAGATTCT GTGCCTGTGC CTGATGAAGT AAGCATGCTA 
ACAGCAATTG CACTTTTCCT GTGGTCTGCT GGTAGTGAAA TAATAGGAGT TCAGTCACTG 
CAGAATGGCT GCATTTACAG ATTTAAAAAT GCGTTAAATT CATGTGACCC GTGGGTTCAA 
GCCAAATGCT ACCAGCTTCT CCTCTCAGTT TTTCAACATT CAAATCGGGC CCTTTCAACT 
CCTTATATCC ATTCCTTAGC TCCAATAGTA GTTGAAAAGC TAAAAGCTGT TGAAAGAAAC 
AGACCAGGCA GTAGTACAGA ACTCTTAGCT GTTCAAGAAG GAATCAAAGT TCTTGAAACA 
TTGGTTGCTC TTGGGGAAGA ACAGAACAGA GTCCAATTAC TTGCTCTTTT AGTTCCCACT 
TTGATCTCTT ACCTGCTGGA TGAAAATTCT TTTGCCTCAG CAAGCACAGC TTCCAAAGAT 
CTCCATGAGT TTGCACTCCA GAATTTAATG CATATTGGAC CTCTGTATCC ACACGCTTTC 
AAGACAGTAA TGGGAGCAGC TCCTGAGCTG AAAGTACGTC TGGAAACTGC TGTTAGAGCA 
AGTCAAGCTA GTAAGGCTAA AGTGGCCACC AGGCAGCCAG CCCCAACCGT GCACTCTGCA 
CCAACAATTA AGCTAAAAAC AAGTTTCTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_015465330.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320816.1 Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X1, mRNA.

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CloneID	OBa23993
Clone ID Related Accession (Same CDS sequence)	XM_002691283.5 , XM_005212706.3 , XM_002691283.6 , XM_005212706.4 , XM_015465330.1 , XM_015465331.1
Accession Version	XM_015465330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	HEAT repeat-containing protein 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGAAGATTCT GTGCCTGTGC CTGATGAAGT AAGCATGCTA 
ACAGCAATTG CACTTTTCCT GTGGTCTGCT GGTAGTGAAA TAATAGGAGT TCAGTCACTG 
CAGAATGGCT GCATTTACAG ATTTAAAAAT GCGTTAAATT CATGTGACCC GTGGGTTCAA 
GCCAAATGCT ACCAGCTTCT CCTCTCAGTT TTTCAACATT CAAATCGGGC CCTTTCAACT 
CCTTATATCC ATTCCTTAGC TCCAATAGTA GTTGAAAAGC TAAAAGCTGT TGAAAGAAAC 
AGACCAGGCA GTAGTACAGA ACTCTTAGCT GTTCAAGAAG GAATCAAAGT TCTTGAAACA 
TTGGTTGCTC TTGGGGAAGA ACAGAACAGA GTCCAATTAC TTGCTCTTTT AGTTCCCACT 
TTGATCTCTT ACCTGCTGGA TGAAAATTCT TTTGCCTCAG CAAGCACAGC TTCCAAAGAT 
CTCCATGAGT TTGCACTCCA GAATTTAATG CATATTGGAC CTCTGTATCC ACACGCTTTC 
AAGACAGTAA TGGGAGCAGC TCCTGAGCTG AAAGTACGTC TGGAAACTGC TGTTAGAGCA 
AGTCAAGCTA GTAAGGCTAA AGTGGCCACC AGGCAGCCAG CCCCAACCGT GCACTCTGCA 
CCAACAATTA AGCTAAAAAC AAGTTTCTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320816.1
CDS	109..6321
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKKLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIRNKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKSAESQGRSEILMSLQKVLNGLGGAAASCHRDIYKNARSLLTDRSMAVRCAVAKCLLELQNEAVFMWTAELENIATLCFKALENSNYGVRVAVSKLLGTVMATALMPKQATVMRQNVKRATFDEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSGENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNSLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSFKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSSEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKSSGDKESSGTNVSPFAPGASSRSDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVEKLSHWLMLCKDVLAASSDMSTATPLSSGKDEEFEKKDEMDDDTMFTTLGEEDKSKPFVAPRWATRVFAADCLCRIINLCENADQAHFDLAMARSAKLRNPTNDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAESKPKRAVKNTDDDDDDDSTIDELPPDSLITLVQPELPILSRLWLAALKDYALLTLPAEFASQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTCSESAEAAAISALQKRSIPVNLSQASGAIPSAKSLAEINKDRMHLILGVSIQFLCSPRPEEPVEHVTACLQALHTLLDSPYARIHIAEDQLIGVELLSVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDIEKESCPVLGEGGDSGGLIPGKSLVFATLELLMFILVRHMPHLSTKMSDSPSHIATKTRLSEESARLVAATVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSADNQVPPPVSAALQGIKSIVTLSMAKTEDTQKQWTALIRSTLACILEYSQPEDSVPVPDEVSMLTAIALFLWSAGSEIIGVQSLQNGCIYRFKNALNSCDPWVQAKCYQLLLSVFQHSNRALSTPYIHSLAPIVVEKLKAVERNRPGSSTELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENSFASASTASKDLHEFALQNLMHIGPLYPHAFKTVMGAAPELKVRLETAVRASQASKAKVATRQPAPTVHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_015465330.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465330.1

ORF Insert Sequence:

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGAAGATTCT GTGCCTGTGC CTGATGAAGT AAGCATGCTA 
ACAGCAATTG CACTTTTCCT GTGGTCTGCT GGTAGTGAAA TAATAGGAGT TCAGTCACTG 
CAGAATGGCT GCATTTACAG ATTTAAAAAT GCGTTAAATT CATGTGACCC GTGGGTTCAA 
GCCAAATGCT ACCAGCTTCT CCTCTCAGTT TTTCAACATT CAAATCGGGC CCTTTCAACT 
CCTTATATCC ATTCCTTAGC TCCAATAGTA GTTGAAAAGC TAAAAGCTGT TGAAAGAAAC 
AGACCAGGCA GTAGTACAGA ACTCTTAGCT GTTCAAGAAG GAATCAAAGT TCTTGAAACA 
TTGGTTGCTC TTGGGGAAGA ACAGAACAGA GTCCAATTAC TTGCTCTTTT AGTTCCCACT 
TTGATCTCTT ACCTGCTGGA TGAAAATTCT TTTGCCTCAG CAAGCACAGC TTCCAAAGAT 
CTCCATGAGT TTGCACTCCA GAATTTAATG CATATTGGAC CTCTGTATCC ACACGCTTTC 
AAGACAGTAA TGGGAGCAGC TCCTGAGCTG AAAGTACGTC TGGAAACTGC TGTTAGAGCA 
AGTCAAGCTA GTAAGGCTAA AGTGGCCACC AGGCAGCCAG CCCCAACCGT GCACTCTGCA 
CCAACAATTA AGCTAAAAAC AAGTTTCTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_015465331.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320817.1 Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X2, mRNA.

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CloneID	OBa23993
Clone ID Related Accession (Same CDS sequence)	XM_002691283.5 , XM_005212706.3 , XM_002691283.6 , XM_005212706.4 , XM_015465330.1 , XM_015465331.1
Accession Version	XM_015465331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	HEAT repeat-containing protein 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGAAGATTCT GTGCCTGTGC CTGATGAAGT AAGCATGCTA 
ACAGCAATTG CACTTTTCCT GTGGTCTGCT GGTAGTGAAA TAATAGGAGT TCAGTCACTG 
CAGAATGGCT GCATTTACAG ATTTAAAAAT GCGTTAAATT CATGTGACCC GTGGGTTCAA 
GCCAAATGCT ACCAGCTTCT CCTCTCAGTT TTTCAACATT CAAATCGGGC CCTTTCAACT 
CCTTATATCC ATTCCTTAGC TCCAATAGTA GTTGAAAAGC TAAAAGCTGT TGAAAGAAAC 
AGACCAGGCA GTAGTACAGA ACTCTTAGCT GTTCAAGAAG GAATCAAAGT TCTTGAAACA 
TTGGTTGCTC TTGGGGAAGA ACAGAACAGA GTCCAATTAC TTGCTCTTTT AGTTCCCACT 
TTGATCTCTT ACCTGCTGGA TGAAAATTCT TTTGCCTCAG CAAGCACAGC TTCCAAAGAT 
CTCCATGAGT TTGCACTCCA GAATTTAATG CATATTGGAC CTCTGTATCC ACACGCTTTC 
AAGACAGTAA TGGGAGCAGC TCCTGAGCTG AAAGTACGTC TGGAAACTGC TGTTAGAGCA 
AGTCAAGCTA GTAAGGCTAA AGTGGCCACC AGGCAGCCAG CCCCAACCGT GCACTCTGCA 
CCAACAATTA AGCTAAAAAC AAGTTTCTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320817.1
CDS	188..6400
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKKLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIRNKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKSAESQGRSEILMSLQKVLNGLGGAAASCHRDIYKNARSLLTDRSMAVRCAVAKCLLELQNEAVFMWTAELENIATLCFKALENSNYGVRVAVSKLLGTVMATALMPKQATVMRQNVKRATFDEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSGENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNSLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSFKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSSEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKSSGDKESSGTNVSPFAPGASSRSDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVEKLSHWLMLCKDVLAASSDMSTATPLSSGKDEEFEKKDEMDDDTMFTTLGEEDKSKPFVAPRWATRVFAADCLCRIINLCENADQAHFDLAMARSAKLRNPTNDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAESKPKRAVKNTDDDDDDDSTIDELPPDSLITLVQPELPILSRLWLAALKDYALLTLPAEFASQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTCSESAEAAAISALQKRSIPVNLSQASGAIPSAKSLAEINKDRMHLILGVSIQFLCSPRPEEPVEHVTACLQALHTLLDSPYARIHIAEDQLIGVELLSVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDIEKESCPVLGEGGDSGGLIPGKSLVFATLELLMFILVRHMPHLSTKMSDSPSHIATKTRLSEESARLVAATVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSADNQVPPPVSAALQGIKSIVTLSMAKTEDTQKQWTALIRSTLACILEYSQPEDSVPVPDEVSMLTAIALFLWSAGSEIIGVQSLQNGCIYRFKNALNSCDPWVQAKCYQLLLSVFQHSNRALSTPYIHSLAPIVVEKLKAVERNRPGSSTELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENSFASASTASKDLHEFALQNLMHIGPLYPHAFKTVMGAAPELKVRLETAVRASQASKAKVATRQPAPTVHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_015465331.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465331.1

ORF Insert Sequence:

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGAAGATTCT GTGCCTGTGC CTGATGAAGT AAGCATGCTA 
ACAGCAATTG CACTTTTCCT GTGGTCTGCT GGTAGTGAAA TAATAGGAGT TCAGTCACTG 
CAGAATGGCT GCATTTACAG ATTTAAAAAT GCGTTAAATT CATGTGACCC GTGGGTTCAA 
GCCAAATGCT ACCAGCTTCT CCTCTCAGTT TTTCAACATT CAAATCGGGC CCTTTCAACT 
CCTTATATCC ATTCCTTAGC TCCAATAGTA GTTGAAAAGC TAAAAGCTGT TGAAAGAAAC 
AGACCAGGCA GTAGTACAGA ACTCTTAGCT GTTCAAGAAG GAATCAAAGT TCTTGAAACA 
TTGGTTGCTC TTGGGGAAGA ACAGAACAGA GTCCAATTAC TTGCTCTTTT AGTTCCCACT 
TTGATCTCTT ACCTGCTGGA TGAAAATTCT TTTGCCTCAG CAAGCACAGC TTCCAAAGAT 
CTCCATGAGT TTGCACTCCA GAATTTAATG CATATTGGAC CTCTGTATCC ACACGCTTTC 
AAGACAGTAA TGGGAGCAGC TCCTGAGCTG AAAGTACGTC TGGAAACTGC TGTTAGAGCA 
AGTCAAGCTA GTAAGGCTAA AGTGGCCACC AGGCAGCCAG CCCCAACCGT GCACTCTGCA 
CCAACAATTA AGCTAAAAAC AAGTTTCTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_005212707.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212764.1 Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X3, mRNA.

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CloneID	OBa23994
Clone ID Related Accession (Same CDS sequence)	XM_005212707.2 , XM_005212707.3 , XM_015465332.1
Accession Version	XM_005212707.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	HEAT repeat-containing protein 5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005212707.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGATTCTGTG CCTGTGCCTG ATGAAGTAAG CATGCTAACA 
GCAATTGCAC TTTTCCTGTG GTCTGCTGGT AGTGAAATAA TAGGAGTTCA GTCACTGCAG 
AATGGCTGCA TTTACAGATT TAAAAATGCG TTAAATTCAT GTGACCCGTG GGTTCAAGCC 
AAATGCTACC AGCTTCTCCT CTCAGTTTTT CAACATTCAA ATCGGGCCCT TTCAACTCCT 
TATATCCATT CCTTAGCTCC AATAGTAGTT GAAAAGCTAA AAGCTGTTGA AAGAAACAGA 
CCAGGCAGTA GTACAGAACT CTTAGCTGTT CAAGAAGGAA TCAAAGTTCT TGAAACATTG 
GTTGCTCTTG GGGAAGAACA GAACAGAGTC CAATTACTTG CTCTTTTAGT TCCCACTTTG 
ATCTCTTACC TGCTGGATGA AAATTCTTTT GCCTCAGCAA GCACAGCTTC CAAAGATCTC 
CATGAGTTTG CACTCCAGAA TTTAATGCAT ATTGGACCTC TGTATCCACA CGCTTTCAAG 
ACAGTAATGG GAGCAGCTCC TGAGCTGAAA GTACGTCTGG AAACTGCTGT TAGAGCAAGT 
CAAGCTAGTA AGGCTAAAGT GGCCACCAGG CAGCCAGCCC CAACCGTGCA CTCTGCACCA 
ACAATTAAGC TAAAAACAAG TTTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212764.1
CDS	110..6319
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKKLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIRNKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKSAESQGRSEILMSLQKVLNGLGGAAASCHRDIYKNARSLLTDRSMAVRCAVAKCLLELQNEAVFMWTAELENIATLCFKALENSNYGVRVAVSKLLGTVMATALMPKQATVMRQNVKRATFDEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSGENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNSLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSFKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSSEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKSSGDKESSGTNVSPFAPGASSRSDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVEKLSHWLMLCKDVLAASSDMSTATPLSSGKDEEFEKKDEMDDDTMFTTLGEEDKSKPFVAPRWATRVFAADCLCRIINLCENADQAHFDLAMARSAKLRNPTNDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAESKPKRAVKNTDDDDDDDSTIDELPPDSLITLVQPELPILSRLWLAALKDYALLTLPAEFASQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTCSESAEAAAISALQKRSIPVNLSQASGAIPSAKSLAEINKDRMHLILGVSIQFLCSPRPEEPVEHVTACLQALHTLLDSPYARIHIAEDQLIGVELLSVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDIEKESCPVLGEGGDSGGLIPGKSLVFATLELLMFILVRHMPHLSTKMSDSPSHIATKTRLSEESARLVAATVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSADNQVPPPVSAALQGIKSIVTLSMAKTEDTQKQWTALIRSTLACILEYSQPDSVPVPDEVSMLTAIALFLWSAGSEIIGVQSLQNGCIYRFKNALNSCDPWVQAKCYQLLLSVFQHSNRALSTPYIHSLAPIVVEKLKAVERNRPGSSTELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENSFASASTASKDLHEFALQNLMHIGPLYPHAFKTVMGAAPELKVRLETAVRASQASKAKVATRQPAPTVHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_005212707.2
Organism	Bos taurus(cattle)
Definition	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212707.2

ORF Insert Sequence:

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGATTCTGTG CCTGTGCCTG ATGAAGTAAG CATGCTAACA 
GCAATTGCAC TTTTCCTGTG GTCTGCTGGT AGTGAAATAA TAGGAGTTCA GTCACTGCAG 
AATGGCTGCA TTTACAGATT TAAAAATGCG TTAAATTCAT GTGACCCGTG GGTTCAAGCC 
AAATGCTACC AGCTTCTCCT CTCAGTTTTT CAACATTCAA ATCGGGCCCT TTCAACTCCT 
TATATCCATT CCTTAGCTCC AATAGTAGTT GAAAAGCTAA AAGCTGTTGA AAGAAACAGA 
CCAGGCAGTA GTACAGAACT CTTAGCTGTT CAAGAAGGAA TCAAAGTTCT TGAAACATTG 
GTTGCTCTTG GGGAAGAACA GAACAGAGTC CAATTACTTG CTCTTTTAGT TCCCACTTTG 
ATCTCTTACC TGCTGGATGA AAATTCTTTT GCCTCAGCAA GCACAGCTTC CAAAGATCTC 
CATGAGTTTG CACTCCAGAA TTTAATGCAT ATTGGACCTC TGTATCCACA CGCTTTCAAG 
ACAGTAATGG GAGCAGCTCC TGAGCTGAAA GTACGTCTGG AAACTGCTGT TAGAGCAAGT 
CAAGCTAGTA AGGCTAAAGT GGCCACCAGG CAGCCAGCCC CAACCGTGCA CTCTGCACCA 
ACAATTAAGC TAAAAACAAG TTTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_005212707.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212764.1 Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X3, mRNA.

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CloneID	OBa23994
Clone ID Related Accession (Same CDS sequence)	XM_005212707.2 , XM_005212707.3 , XM_015465332.1
Accession Version	XM_005212707.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	HEAT repeat-containing protein 5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212707.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGATTCTGTG CCTGTGCCTG ATGAAGTAAG CATGCTAACA 
GCAATTGCAC TTTTCCTGTG GTCTGCTGGT AGTGAAATAA TAGGAGTTCA GTCACTGCAG 
AATGGCTGCA TTTACAGATT TAAAAATGCG TTAAATTCAT GTGACCCGTG GGTTCAAGCC 
AAATGCTACC AGCTTCTCCT CTCAGTTTTT CAACATTCAA ATCGGGCCCT TTCAACTCCT 
TATATCCATT CCTTAGCTCC AATAGTAGTT GAAAAGCTAA AAGCTGTTGA AAGAAACAGA 
CCAGGCAGTA GTACAGAACT CTTAGCTGTT CAAGAAGGAA TCAAAGTTCT TGAAACATTG 
GTTGCTCTTG GGGAAGAACA GAACAGAGTC CAATTACTTG CTCTTTTAGT TCCCACTTTG 
ATCTCTTACC TGCTGGATGA AAATTCTTTT GCCTCAGCAA GCACAGCTTC CAAAGATCTC 
CATGAGTTTG CACTCCAGAA TTTAATGCAT ATTGGACCTC TGTATCCACA CGCTTTCAAG 
ACAGTAATGG GAGCAGCTCC TGAGCTGAAA GTACGTCTGG AAACTGCTGT TAGAGCAAGT 
CAAGCTAGTA AGGCTAAAGT GGCCACCAGG CAGCCAGCCC CAACCGTGCA CTCTGCACCA 
ACAATTAAGC TAAAAACAAG TTTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212764.1
CDS	133..6342
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKKLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIRNKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKSAESQGRSEILMSLQKVLNGLGGAAASCHRDIYKNARSLLTDRSMAVRCAVAKCLLELQNEAVFMWTAELENIATLCFKALENSNYGVRVAVSKLLGTVMATALMPKQATVMRQNVKRATFDEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSGENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNSLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSFKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSSEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKSSGDKESSGTNVSPFAPGASSRSDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVEKLSHWLMLCKDVLAASSDMSTATPLSSGKDEEFEKKDEMDDDTMFTTLGEEDKSKPFVAPRWATRVFAADCLCRIINLCENADQAHFDLAMARSAKLRNPTNDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAESKPKRAVKNTDDDDDDDSTIDELPPDSLITLVQPELPILSRLWLAALKDYALLTLPAEFASQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTCSESAEAAAISALQKRSIPVNLSQASGAIPSAKSLAEINKDRMHLILGVSIQFLCSPRPEEPVEHVTACLQALHTLLDSPYARIHIAEDQLIGVELLSVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDIEKESCPVLGEGGDSGGLIPGKSLVFATLELLMFILVRHMPHLSTKMSDSPSHIATKTRLSEESARLVAATVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSADNQVPPPVSAALQGIKSIVTLSMAKTEDTQKQWTALIRSTLACILEYSQPDSVPVPDEVSMLTAIALFLWSAGSEIIGVQSLQNGCIYRFKNALNSCDPWVQAKCYQLLLSVFQHSNRALSTPYIHSLAPIVVEKLKAVERNRPGSSTELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENSFASASTASKDLHEFALQNLMHIGPLYPHAFKTVMGAAPELKVRLETAVRASQASKAKVATRQPAPTVHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_005212707.3
Organism	Bos taurus(cattle)
Definition	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212707.3

ORF Insert Sequence:

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGATTCTGTG CCTGTGCCTG ATGAAGTAAG CATGCTAACA 
GCAATTGCAC TTTTCCTGTG GTCTGCTGGT AGTGAAATAA TAGGAGTTCA GTCACTGCAG 
AATGGCTGCA TTTACAGATT TAAAAATGCG TTAAATTCAT GTGACCCGTG GGTTCAAGCC 
AAATGCTACC AGCTTCTCCT CTCAGTTTTT CAACATTCAA ATCGGGCCCT TTCAACTCCT 
TATATCCATT CCTTAGCTCC AATAGTAGTT GAAAAGCTAA AAGCTGTTGA AAGAAACAGA 
CCAGGCAGTA GTACAGAACT CTTAGCTGTT CAAGAAGGAA TCAAAGTTCT TGAAACATTG 
GTTGCTCTTG GGGAAGAACA GAACAGAGTC CAATTACTTG CTCTTTTAGT TCCCACTTTG 
ATCTCTTACC TGCTGGATGA AAATTCTTTT GCCTCAGCAA GCACAGCTTC CAAAGATCTC 
CATGAGTTTG CACTCCAGAA TTTAATGCAT ATTGGACCTC TGTATCCACA CGCTTTCAAG 
ACAGTAATGG GAGCAGCTCC TGAGCTGAAA GTACGTCTGG AAACTGCTGT TAGAGCAAGT 
CAAGCTAGTA AGGCTAAAGT GGCCACCAGG CAGCCAGCCC CAACCGTGCA CTCTGCACCA 
ACAATTAAGC TAAAAACAAG TTTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_015465332.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320818.1 Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X3, mRNA.

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CloneID	OBa23994
Clone ID Related Accession (Same CDS sequence)	XM_005212707.2 , XM_005212707.3 , XM_015465332.1
Accession Version	XM_015465332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	HEAT repeat-containing protein 5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGATTCTGTG CCTGTGCCTG ATGAAGTAAG CATGCTAACA 
GCAATTGCAC TTTTCCTGTG GTCTGCTGGT AGTGAAATAA TAGGAGTTCA GTCACTGCAG 
AATGGCTGCA TTTACAGATT TAAAAATGCG TTAAATTCAT GTGACCCGTG GGTTCAAGCC 
AAATGCTACC AGCTTCTCCT CTCAGTTTTT CAACATTCAA ATCGGGCCCT TTCAACTCCT 
TATATCCATT CCTTAGCTCC AATAGTAGTT GAAAAGCTAA AAGCTGTTGA AAGAAACAGA 
CCAGGCAGTA GTACAGAACT CTTAGCTGTT CAAGAAGGAA TCAAAGTTCT TGAAACATTG 
GTTGCTCTTG GGGAAGAACA GAACAGAGTC CAATTACTTG CTCTTTTAGT TCCCACTTTG 
ATCTCTTACC TGCTGGATGA AAATTCTTTT GCCTCAGCAA GCACAGCTTC CAAAGATCTC 
CATGAGTTTG CACTCCAGAA TTTAATGCAT ATTGGACCTC TGTATCCACA CGCTTTCAAG 
ACAGTAATGG GAGCAGCTCC TGAGCTGAAA GTACGTCTGG AAACTGCTGT TAGAGCAAGT 
CAAGCTAGTA AGGCTAAAGT GGCCACCAGG CAGCCAGCCC CAACCGTGCA CTCTGCACCA 
ACAATTAAGC TAAAAACAAG TTTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320818.1
CDS	110..6319
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKKLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIRNKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKSAESQGRSEILMSLQKVLNGLGGAAASCHRDIYKNARSLLTDRSMAVRCAVAKCLLELQNEAVFMWTAELENIATLCFKALENSNYGVRVAVSKLLGTVMATALMPKQATVMRQNVKRATFDEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSGENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNSLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSFKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSSEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKSSGDKESSGTNVSPFAPGASSRSDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVEKLSHWLMLCKDVLAASSDMSTATPLSSGKDEEFEKKDEMDDDTMFTTLGEEDKSKPFVAPRWATRVFAADCLCRIINLCENADQAHFDLAMARSAKLRNPTNDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAESKPKRAVKNTDDDDDDDSTIDELPPDSLITLVQPELPILSRLWLAALKDYALLTLPAEFASQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTCSESAEAAAISALQKRSIPVNLSQASGAIPSAKSLAEINKDRMHLILGVSIQFLCSPRPEEPVEHVTACLQALHTLLDSPYARIHIAEDQLIGVELLSVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDIEKESCPVLGEGGDSGGLIPGKSLVFATLELLMFILVRHMPHLSTKMSDSPSHIATKTRLSEESARLVAATVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSADNQVPPPVSAALQGIKSIVTLSMAKTEDTQKQWTALIRSTLACILEYSQPDSVPVPDEVSMLTAIALFLWSAGSEIIGVQSLQNGCIYRFKNALNSCDPWVQAKCYQLLLSVFQHSNRALSTPYIHSLAPIVVEKLKAVERNRPGSSTELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENSFASASTASKDLHEFALQNLMHIGPLYPHAFKTVMGAAPELKVRLETAVRASQASKAKVATRQPAPTVHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_015465332.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HEAT repeat containing 5B (HEATR5B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465332.1

ORF Insert Sequence:

ATGGAGTTAG CCCACAGTTT ATTGCTCAAT GAAGAAGCTT TGGCTCAAAT CACTGAAGCA 
AAGAGACCAG TTTTTATCTT TGAATGGTTG CGATTTCTTG ATAAAGTCTT GGTTGCTGCC 
AACAAGACTG ATGTAAAGGA AAAACAGAAA AAACTTGTTG AACAATTAAC TGGATTAATA 
AGTAGTTCAC CTGGACCACC TACACGAAAA TTGTTAGCTA AAAACCTTGC AGCCCTTTAT 
AGCATTGGGG ATACTTTCAC TGTTTTTCAA ACACTTGATA AATGCAATGA CATTATCAGA 
AATAAAGATG ACACTGCAGC CTACTTACCA ACAAAATTGG CTGCAGTGGC TTGTGTTGGA 
GCATTTTATG AAAAAATGGG GAGAATGTTA GGCAGTGCAT TTCCAGAAAC AGTGAATAAT 
CTTTTGAAAT CTCTGAAGAG TGCAGAGTCT CAGGGGCGAA GTGAAATCTT AATGAGTCTA 
CAGAAAGTTC TGAATGGACT GGGTGGTGCC GCAGCTTCCT GTCATCGTGA TATTTACAAG 
AATGCCAGGT CCCTCTTAAC TGACAGGTCA ATGGCTGTTC GATGTGCAGT TGCCAAGTGT 
CTCCTAGAAC TACAGAATGA AGCTGTGTTC ATGTGGACTG CTGAACTGGA AAATATAGCC 
ACTCTCTGCT TCAAGGCTTT GGAAAACTCT AACTATGGGG TACGAGTGGC AGTGTCTAAG 
CTCTTAGGAA CAGTCATGGC TACAGCACTG ATGCCAAAAC AAGCAACAGT AATGCGCCAG 
AATGTGAAGC GAGCAACCTT TGATGAAGTC TTAGAACTCA TGGCCACAGG ATTTCTGCGT 
GGAGGGTCAG GTTTCTTAAA GAGTGGTGGA GAAATGTTAA AAGTTGGAGG GTCTGTTAAT 
CGTGAAGTGA GAGTTGGAGT TACCCAGGCA TATGTTGTTT TTGTAACAAC ATTGGGTGGC 
CAGTGGTTGG AGCGTAGTTT TGCTACATTC TTGTCTCATG TACTTGATCT GGTTTCCCAT 
CCTCGGGCAA CACAAACACA TGTGGAGGCT GTGTATTCCA GACGATGTGT CTCCTTTATT 
TTAAGAGCTA CCGTGGGCAG TTTGCTAGGT GAAAAAGCCC AGATTGCAGC TGCCAAAGAA 
ATATGCCAGG CGATTGGAAA ACAAATGAAA GCAGTAGAAG CGGTAGTGAA TGACACTAGT 
GGGGAGAACA AGTCTGGAGC AGCAGACATT GCAGCAAGCC AGCATGTGAT GGTCTGTGCC 
CTGCAGGAGC TCGGGAGCCT GGTGCAGAGC CTGAATGCCA CCGCATCCCC TCTTATTCAG 
GAAGCATCTA TAGGCCTTTT GGAGACTGTG ACCTCAGTGC TTCTCCATCC AAGCATGGCA 
GCTCGCCTGG CTGCTGCCTG GTGTTTGCGC TGTGTGGCTG TGGCATTACC TTTCCAGCTG 
ACGCCATTTC TAGACAGATG TGCAGAACGG CTCAACAGCT TAAAAACCTC GCCAGAAGCT 
GTTAGTGGAT ACAGTTTTGC AATGGCTGCT TTGTTAGGTG GAGTTCATCA GTGTCCTTTG 
GGCATTCCTC ATGCCAAGGG AAAGATGGTT GTTAGTATTG CTGAAGATCT CTTACGAACT 
GCTGCCCAAA ATAGCAGGCT GTCTTTACAG CGCACCCAAG CTGGATGGCT CTTACTTGGA 
GCACTCATGA CTTTAGGACC GTCTGTTGTT CGTTACCATC TACCCAAGAT GTTGTTATTG 
TGGCGAAACG TTTTCCCTCG TTCTTTAAAG GAATTGGAGG CTGAGAAGGC CCGAGGCGAT 
TCTTTTACCT GGCAGGTAAC ATTGGAAGGT CGTGCTGGAG CTTTATGTGC AATGAGGAGT 
TTTGTTGCAC ATTGCCCTGA ACTCCTAACT GAAGATGTGA TTAGAAAATT GATGACCCCT 
ATTGAATGTG CCATGACTAT GATGTCACAC ATTCCCTCTG TAATTAAAGC CCATGGAGCT 
CATCTGAAAG CTAGTGCTGC CATGGTCCGT TTAAGGCTTT ATGATATCTT GGCATTGTTA 
CCTCCAAAAA CTTATGAAGG CTCTTTCAAT GCACTTCTTA GAGAACTGGT GGCAGAATTC 
ACTTTGACTG ACAACTCAGC CAACACAACT ACTTCCCTCC TCAGATCCCT CTGCCACTAT 
GATGATAGTG TTCTACTTGG GTCCTGGCTT CAGGAAACTG ATCATAAATC AATTGAAGAC 
CAGCTCCAGC CAAACAGTGC ATCTGGGAGT GGGGCTTTGG AGCATGACCC ATCCTCCATT 
TACTTACGAA TACCTGCTGG AGAAGCTGTG CCTGGGCCTC TTCCCCTTGG AGTTTCGGTC 
ATTGACGCTT CCGTGGCTCT TTTTGGTGTA GTGTTTCCTC ATGTTTCTTT TAAACACCGA 
TTACAGATGT TGGATCACTT TGCTGAATGT GTTAAACAAG CCAAAGGTGT TCGCCAGCAG 
GCGGTGCAGC TTAATATCTT TACAGCTGTA CTTAGTGCAC TAAAGGGCCT CGCTGAAAAC 
AAAAGTACTT TAGGACCAGA GGAAGTTCGT AAATCTGCTC TGACACTGGT TATGGGGGCC 
CTGGACAATC CAAACCCCAT CTTACGATGT GCAGCAGGGG AAGCTCTTGG AAGAATGGCT 
CAGGTGGTCG GAGAAGCAAC TTTTATTGCT AGAATGGCCC AATATAGCTT TGACAAGTTG 
AAGTCAGCTC GAGACGTTGT GTCCAGAACC GGCCACTCAT TGGCTCTGGG CTGTTTGCAT 
CGTTATGTTG GTGGTATCGG CTCAGGACAA CATCTGAAGA CCAGTGTCAG CATTTTGTTG 
GCCCTGGCAC AGGATGGAAC ATCCTCTGAA GTCCAGACTT GGTCCCTTCA CTCGCTTGCT 
TTGATAGTGG ATTCTAGTGG CCCAATGTAT CGTGGATACG TAGAGCCAAC GTTATCTCTA 
GTTCTTACCT TGCTGTTGAC AGTTCCACCC TCACATACAG AAGTTCATCA GTGTTTGGGG 
AGATGCTTGG GTGCTATAAT AACTACTGTT GGACCGGAAC TACAAGGGAA TGGAGCAACA 
ATTTCCACAA TCCGTTCTTC TTGTTTGGTG GGTTGTGCGA TAACCCAGGA CCATTCAGAT 
TCTCTGGTCC AGGCAGCTGC CATATCTTGC CTTCAGCAGC TTCACATGTT TGCACCACGA 
CATGTCAATT TATCCAGTCT TGTTCCTAGC CTTTGTGTTC ACCTGTGTAG TTCCCATCTA 
TTACTTCGAC GAGCAGCTGT GGCCTGTCTC CGGCAACTTG CTCAAAGAGA AGCAGCTGAA 
GTATGTGAAT ACGCCATGAG CCTGGCAAAA AGTTCAGGGG ACAAAGAGAG CAGTGGTACC 
AATGTTAGTC CTTTTGCGCC CGGAGCAAGT TCTCGAAGTG ATATCCATTG CCGGCATCAA 
GGTGTTAATA TAACAGAAAC TGGTCTCGAG GGACTTCTTT TTGGAATGCT AGACCGGGAG 
ACAGACAGAA AATTATGTTC TGATATCCAT GATACTTTGG GACATATGCT GTCATCGCTG 
GCTGTAGAAA AGCTTTCTCA TTGGCTAATG CTTTGTAAAG ATGTCCTAGC AGCTTCTAGT 
GATATGAGTA CTGCAACTCC CTTAAGCAGT GGAAAAGATG AGGAATTTGA GAAGAAAGAT 
GAGATGGATG ATGATACCAT GTTTACCACA CTCGGAGAAG AAGATAAATC AAAGCCCTTT 
GTGGCACCTC GCTGGGCCAC TCGAGTATTT GCTGCTGATT GCCTGTGTCG AATCATCAAT 
CTGTGTGAGA ATGCAGACCA AGCTCACTTT GATCTTGCCA TGGCACGTTC TGCTAAACTA 
CGAAACCCTA CAAATGACCT CTTGGTACTT CATCTCTCTG ACCTAATTCG CATGGCATTC 
ATGGCTGCAA CCGATCATAG TAACCAGCTG CGGATGGCTG GTCTCCAAGC CCTCGAAGAC 
ATTATCAAGA AGTTTGCCTC TGTGCCTGAG CCAGAATTCC CAGGTCATGT GATTCTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTCTA AGACCAGCCT TTTCACAAGA TACCCCATCA 
GACATAATAG CAAAAGCTTG CCAGGTGTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT CTGCTTGTTT CTTCACTGGA CAAAGTTCAG 
GCCGGAAAAG GATCTTCCAG CCAACTGTAC CGAGAGAGTG CTACAACCAT GGAAAAACTG 
GCTGTTCTCA AAGCATGGGC AGAGGTCTAT GTGGTTGCTA TGAATATTAA AAAAGAAGCA 
GAGTCAAAAC CAAAAAGAGC AGTTAAAAAT ACTGATGACG ATGATGATGA TGACAGTACC 
ATAGATGAAC TACCACCAGA TAGTTTAATA ACACTGGTAC AGCCTGAACT GCCAATACTT 
AGTCGCCTGT GGTTGGCAGC ATTAAAAGAC TACGCGCTTT TAACTTTACC AGCTGAATTC 
GCTAGTCAAC TCCCTCCAGA TGGTGGGGCA TTTTATACTC CTGAGACTAT TGATACGGCT 
AGACTGCACT ATCGGAATTC CTGGGCCCCA ATTCTCCACG CGGTGGCACT TTGGTTAAAC 
AGCACAGGAT TTACATGTTC AGAGTCAGCA GAAGCAGCAG CAATATCCGC TTTACAAAAA 
CGTTCCATAC CGGTCAATTT AAGCCAAGCA TCAGGAGCAA TACCTAGTGC TAAATCTTTG 
GCAGAAATTA ACAAAGACAG AATGCATCTG ATTTTAGGTG TGAGTATACA GTTCCTTTGT 
TCCCCAAGAC CTGAGGAACC TGTTGAACAT GTTACAGCTT GTCTACAGGC CTTGCACACC 
TTGCTAGATT CTCCTTATGC CCGAATCCAT ATTGCAGAAG ATCAGCTGAT TGGTGTTGAG 
TTACTGAGTG TTTTACACCG CCTCCTGTTG ACCTGGAATC CACCGTCTGT CCAGCTGCTG 
GTGACTGGAG TTGTACAACA GATAGTAAGA GCTGCTCAGG ATTATTTGCA AGAAAAAAGA 
AACACACTAA ATGAGGATGA TATAGAAAAA GAGTCCTGTC CTGTATTAGG AGAAGGAGGT 
GACAGTGGTG GTCTCATTCC TGGAAAATCT CTTGTGTTTG CAACTCTGGA GCTGTTGATG 
TTCATTTTAG TACGTCATAT GCCACATCTC AGTACCAAGA TGTCGGACTC TCCAAGTCAC 
ATAGCCACTA AAACTCGACT GTCGGAAGAA AGTGCTCGTT TGGTGGCAGC CACAGTTACC 
ATACTCTCCG ACTTACCGTC TCTCTGTTCA CCTGCTGGAT GTATGACAAT CTTGCCCACA 
ATTCTGTTTT TAATTGCAAG AATATTGAAA GACACAGCAA TAAAGTCTGC AGATAATCAG 
GTTCCTCCAC CAGTCAGTGC AGCTCTTCAA GGGATCAAAA GTATTGTGAC ACTTTCAATG 
GCCAAAACTG AGGATACTCA AAAACAGTGG ACAGCTCTAA TTCGTAGCAC TCTGGCATGC 
ATCCTAGAAT ATTCCCAACC AGATTCTGTG CCTGTGCCTG ATGAAGTAAG CATGCTAACA 
GCAATTGCAC TTTTCCTGTG GTCTGCTGGT AGTGAAATAA TAGGAGTTCA GTCACTGCAG 
AATGGCTGCA TTTACAGATT TAAAAATGCG TTAAATTCAT GTGACCCGTG GGTTCAAGCC 
AAATGCTACC AGCTTCTCCT CTCAGTTTTT CAACATTCAA ATCGGGCCCT TTCAACTCCT 
TATATCCATT CCTTAGCTCC AATAGTAGTT GAAAAGCTAA AAGCTGTTGA AAGAAACAGA 
CCAGGCAGTA GTACAGAACT CTTAGCTGTT CAAGAAGGAA TCAAAGTTCT TGAAACATTG 
GTTGCTCTTG GGGAAGAACA GAACAGAGTC CAATTACTTG CTCTTTTAGT TCCCACTTTG 
ATCTCTTACC TGCTGGATGA AAATTCTTTT GCCTCAGCAA GCACAGCTTC CAAAGATCTC 
CATGAGTTTG CACTCCAGAA TTTAATGCAT ATTGGACCTC TGTATCCACA CGCTTTCAAG 
ACAGTAATGG GAGCAGCTCC TGAGCTGAAA GTACGTCTGG AAACTGCTGT TAGAGCAAGT 
CAAGCTAGTA AGGCTAAAGT GGCCACCAGG CAGCCAGCCC CAACCGTGCA CTCTGCACCA 
ACAATTAAGC TAAAAACAAG TTTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	HEATR5B
Entrez Gene ID	540503
Full Name	HEAT repeat containing 5B
General protein information	Preferred Names HEAT repeat containing 5B Names HEAT repeat-containing protein 5B HEAT repeat-containing protein 5B-like
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	HEATR5B	XP_515406.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002215	XP_307970.5
Caenorhabditis elegans (roundworm)	C13F10.4	NP_504828.1
Mus musculus (house mouse)	Heatr5b	NP_001074648.1
Rattus norvegicus (Norway rat)	Heatr5b	NP_001177993.1
Drosophila melanogaster (fruit fly)	CG2747	NP_001262363.1
	HEATR5B	XP_002799261.1
Xenopus tropicalis (tropical clawed frog)	heatr5b	NP_001090701.1
Canis lupus familiaris (dog)	HEATR5B	XP_540148.2
Gallus gallus (chicken)	HEATR5B	XP_419518.2
Danio rerio (zebrafish)	heatr5b	XP_001920896.3
Homo sapiens (human)	HEATR5B	NP_061897.1
Bos taurus (cattle)	LOC540503	XP_005212763.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

HEATR5B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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