ANK2 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015463691.1	XP_015319177.1	LOW QUALITY PROTEIN: ankyrin-2 isoform X2
XM_024993491.1	XP_024849259.1	ankyrin-2 isoform X7
XM_024993492.1	XP_024849260.1	ankyrin-2 isoform X8
XM_024993496.1	XP_024849264.1	ankyrin-2 isoform X12
XM_024993485.1	XP_024849253.1	ankyrin-2 isoform X1
XM_024993486.1	XP_024849254.1	ankyrin-2 isoform X2
XM_024993493.1	XP_024849261.1	ankyrin-2 isoform X9
XM_024993495.1	XP_024849263.1	ankyrin-2 isoform X11
XM_024993498.1	XP_024849266.1	ankyrin-2 isoform X14
XM_024993487.1	XP_024849255.1	ankyrin-2 isoform X3
XM_024993489.1	XP_024849257.1	ankyrin-2 isoform X5
XM_024993490.1	XP_024849258.1	ankyrin-2 isoform X6
XM_024993494.1	XP_024849262.1	ankyrin-2 isoform X10
XM_024993497.1	XP_024849265.1	ankyrin-2 isoform X13
XM_010805941.2	XP_010804243.1	ankyrin-2 isoform X1
XM_024993488.1	XP_024849256.1	ankyrin-2 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa235482	XM_024993490.1 Latest version!	Bos taurus ankyrin 2 (ANK2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa235483	XM_024993491.1 Latest version!	Bos taurus ankyrin 2 (ANK2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa235484	XM_024993492.1 Latest version!	Bos taurus ankyrin 2 (ANK2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa235485	XM_024993493.1 Latest version!	Bos taurus ankyrin 2 (ANK2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa235486	XM_024993494.1 Latest version!	Bos taurus ankyrin 2 (ANK2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa235487	XM_024993495.1 Latest version!	Bos taurus ankyrin 2 (ANK2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa235488	XM_024993496.1 Latest version!	Bos taurus ankyrin 2 (ANK2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa235489	XM_024993497.1 Latest version!	Bos taurus ankyrin 2 (ANK2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa235490	XM_024993498.1 Latest version!	Bos taurus ankyrin 2 (ANK2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa235478	XM_024993486.1 Latest version!	Bos taurus ankyrin 2 (ANK2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa235479	XM_024993487.1 Latest version!	Bos taurus ankyrin 2 (ANK2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa235480	XM_024993488.1 Latest version!	Bos taurus ankyrin 2 (ANK2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa235481	XM_024993489.1 Latest version!	Bos taurus ankyrin 2 (ANK2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa235477	XM_024993485.1 Latest version!	Bos taurus ankyrin 2 (ANK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa54238	XM_010805941.2 Latest version!	Bos taurus ankyrin-2 (LOC539238), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa90060	XM_015463691.1 Latest version!	Bos taurus ankyrin-2 (LOC539238), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ANK2 ( XM_024993490.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849258.1 Bos taurus ankyrin 2 (ANK2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa235482
Clone ID Related Accession (Same CDS sequence)	XM_024993490.1
Accession Version	XM_024993490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
AGTGGATTCA CCCCTTTGCA CATCGCTGCC CACTATGGAA ACGTCAACGT GGCAACTCTC 
CTTCTAAACC GGGGAGCTGC TGTGGACTTC ACAGCCAGGA ATGGAATCAC TCCTCTGCAT 
GTGGCCTCCA AAAGGGGAAA CACAAACATG GTGAAGCTCT TACTGGATCG TGGTGGTCAG 
ATTGATGCCA AAACTAGGGA TGGGTTGACA CCCCTTCATT GTGCTGCAAG AAGCGGTCAT 
GACCAAGTGG TGGAGCTTCT GCTGGAGCGG GGCGCTCCCT TGCTGGCAAG GACGAAGAAC 
GGGCTGTCTC CACTTCACAT GGCTGCACAG GGGGACCACG TGGAATGTGT GAAGCACCTG 
CTCCAGCACA AGGCGCCTGT TGATGACGTC ACTTTGGACT ACTTGACGGC CCTCCACGTT 
GCCGCACACT GCGGCCACTA CCGAGTAACC AAACTTCTTC TGGACAAGAG AGCCAACCCG 
AACGCCAGAG CCCTGAATGG TTTTACTCCA CTGCACATTG CCTGCAAGAA AAACCGCATC 
AAAGTCATGG AACTGCTGGT GAAATATGGG GCTTCAATCC AAGCTATAAC AGAGTCTGGC 
CTCACACCAA TACATGTGGC TGCCTTCATG GGCCACTTGA ACATTGTCCT CCTTCTGCTG 
CAGAACGGAG CCTCTCCAGA TGTCACTAAC ATTCGTGGGG AGACGGCCCT GCACATGGCG 
GCCCGTGCAG GGCAGGTGGA AGTGGTCCGA TGCCTCCTGA GGAACGGTGC CCTCGTCGAT 
GCCAGAGCCA GGGAGGAACA GACGCCTTTG CATATTGCCT CCCGCCTGGG TAAGACAGAA 
ATTGTTCAGC TGCTTCTGCA ACATATGGCT CATCCAGATG CGGCCACTAC AAATGGGTAC 
ACGCCGTTGC ATATCTCTGC AAGGGAAGGA CAGGTGGACG TGGCCTCGGT CCTCCTGGAA 
GCGGGGGCAG CCCACTCCTT GGCTACCAAG AAGGGTTTTA CCCCCTTGCA CGTAGCTGCC 
AAGTATGGGA GCCTGGATGT GGCAAAACTT CTCTTGCAAC GCCGTGCTGC CGCAGATTCT 
GCGGGGAAGA ATGGCCTTAC CCCGCTCCAT GTTGCTGCTC ACTATGACAA CCAGAAGGTG 
GCGCTGCTGT TACTGGAGAA GGGTGCTTCC CCTCACGCCA TGGCCAAGAA TGGCTACACT 
CCTTTACATA TTGCTGCCAA GAAGAATCAA ATGCAGATAG CTTCCACACT CCTGAGCTAC 
GGAGCAGAGA CGAACATCGT GACAAAGCAA GGAGTCACTC CACTCCATCT GGCCTCGCAG 
GAGGGACACA CGGATATGGT CACCTTGCTT CTGGATAAGG GAGCCAATAT CCACATGTCA 
ACCAAGAGTG GACTCACATC TTTACACCTT GCAGCCCAGG AAGATAAAGT GAATGTCGCT 
GATATTCTCA CCAAGCATGG GGCTGACCAG GATGCGCACA CAAAGCTTGG TTACACTCCT 
TTAATTGTGG CCTGTCACTA TGGAAACGTG AAAATGGTCA ACTTCCTTCT GAAGCAGGGA 
GCAAATGTCA ATGCAAAAAC CAAGAATGGC TACACGCCTT TGCACCAAGC CGCCCAACAG 
GGCCACACGC ACATCATCAA TGTTTTGCTG CAGCACGGGG CCAAGCCCAA CGCTACCACC 
GCGAATGGCA ACACTGCCTT GGCGATTGCT AAGCGTCTGG GCTACATCTC GGTGGTGGAC 
ACCCTGAAGG TTGTAACGGA GGAGGTCACC ACCACCACCA CGACTATCAC TGAAAAACAC 
AAGCTAAATG TTCCTGAGAC CATGACTGAG GTTCTTGATG TTTCTGATGA AGAGGGTGAT 
GACACAATGA CGGGTGATGG GGGAGAGTAC CTTAGACCTG AGGACCTAAA GGAGCTGGGT 
GATGACTCCC TCCCCAGCAG TCAGTTCCTG GACGGGATGA ATTACCTGCG CTACAGCTTG 
GAGGGGGGCC GCTCCGACAG CCTTCGATCC TTCAGTTCCG ACAGGTCTCA CACCCTGAGC 
CATGCCTCGT ACCTGAGGGA CAGTGCCATG ATCGACGACG CAGTTGTGAT CCCCAGTCAC 
CAGGTGACAA CTCTAGCCAA GGAGGCAGAA AGGAATTCTT ATCGTCTAAG CTGGGGCACT 
GAGAACTTGG ACAACGTGGC TCTTTCTTCC AGCCCTATTC ATTCAGGTTT CCTGGTTAGT 
TTTATGGTGG ATGCCCGAGG TGGTGCTATG CGAGGATGCA GACACAACGG GCTCCGCATC 
ATTATTCCGC CCCGGAAATG TACCGCCCCA ACACGAGTCA CCTGCCGACT GGTCAAACGC 
CATAGACTGG CGACAATGCC TCCAATGGTG GAAGGAGAGG GCCTGGCCAG TCGCCTGATT 
GAAGTTGGAC CTTCTGGTGC TCAGTTCCTT GGGCCTGTGA TCGTGGAGAT CCCTCATTTC 
GCTGCCCTGC GAGGAAAAGA GAGGGAACTG GTGGTCCTGC GCAGTGAGAA CGGGGACAGC 
TGGAAAGAGC ATTACTGTGA ATACACGGAG GAGGAGCTGA ATGAAATCCT GAACGGCATG 
GATGAAGTGC TGGATAGCCC GGAAGACCTC GAAAAGAAGC GGATCTGCCG CATCATCACC 
CGTGACTTCC CACAGTACTT TGCGGTGGTG TCGCGCATCA AGCAGGACAG CAACCTGATT 
GGCCCGGAGG GAGGCGTGCT GAGCAGCACC GTGGTGCCGC AGGTGCAGGC CGTGTTCCCC 
GAGGGGGCGC TAACCAAGCG GATCCGTGTT GGCCTACAGG CTCAACCTAT GCACAGTGAG 
TTGGTTAAGA AGATTCTGGG CAACAAAGCT ACCTTCAGCC CTATAGTTAC TTTGGAACCT 
AGAAGAAGAA AATTTCACAA GCCAATTACC ATGACCATTC CTGTCCCCAA AGCTTCAAGT 
GATGTCATGT TGAATGGTTT TGGAGGAGAT GCACCAACCT TAAGATTACT ATGTAGCATC 
ACAGGTGGAA CCACCCCTGC TCAGTGGGAA GATATTACAG GAACTACGCC ATTAACATTT 
GTCAATGAAT GTGTTTCCTT TACAACCAAT GTGTCTGCCA GGTTCTGGCT GATCGATTGT 
CGACAAATTC AGGAATCTGT TACCTTTGCA TCACAAGTGT ATAGAGAAAT TATCTGTGTA 
CCATATATGG CCAAATTTGT AGTGTTTGCC AAATCACATG ACCCCATTGA AGCCAGGTTG 
AGGTGTTTCT GCATGACTGA TGATAAAGTG GATAAAACCC TGGAACAACA AGAAAACTTT 
GCTGAGGTGG CCAGAAGCAG GGATGTGGAG GTCTTAGAAG GAAAACCCAT CTATGTTGAT 
TGTTTTGGCA ACCTGGTGCC ATTAACCAAG AGTGGCCAAC ATCATATATT CAGTTTTTTT 
GCCTTCAAAG AAAACAGACT TCCTCTCTTT GTCAAGGTAC GTGATACGAC TCAGGAACCT 
TGCGGACGAC TATCATTTAT GAAAGAGCCA AAATCTACAA GAGGCCTGGT GCACCAAGCT 
ATTTGCAACT TAAACATCAC CCTGCCAATT TATACGAAGG AATCAGAGTC AGATCAAGAA 
CAGGAGGAAG AGATCGATAT GACATCAGAA AAAAATCAAC AGGATGAGCA GGAACGGATT 
GAGGAAAGGC TGGCATATAT TGCTGATCAC CTTGGCTTCA GCTGGACAGA GTTGGCAAGA 
GAACTGGACT TCACTGAAGA GCAAATTCAT CAAATTCGAA TTGAGAACCC TAACTCCCTG 
CAGGATCAGA GTCATGCGCT GTTGAAGTAC TGGCTAGAAA GGGACGGGAG ACATGCTACA 
GATACCAGTC TCGTTGAATG TCTCACCAAG ATCAACCGAA TGGATATCGT GCATCTCATG 
GAGACCAGCA CAGAAGCTCT CCAGGAGCGC ATCGGTCATA GCTATGCAGA GATTGAGCAG 
ACCATTACAC TGGATCATAG TGAAGGGTTC TCAGTACTCC AAGAGGAGCT TTGCACTGCC 
CAGCACAAGC AGAAAGAGGA GCATGCTGCT TCTAAAGAGG ACGAGGCCTC TGACCACCCT 
CCCATCGTCT CAGAGGAAGA CATTTCTGTT GGTTACTCCA CTCTTCAGGA TGGCATCCCC 
AAAACTGAGG GTGATGGCTC TGCAACAGAG CTCCTTCCCC AGACTCAAAA GGAGCAAGTT 
CAACAGGATT TCTCAGGGAA AATGCAAGAC CTGCCTGAGG AGTCGGCTCT TGAACAGCAA 
GAATACTTCG TGACAACCCC AGGGACAGAA ATGTCAGAGA CTCAGAAGGC CACGGGAACA 
TCGGGCTCCC CCACCAAGAC ACCTGAGGAA ATTGTCACCC CTTCCGAGGA GGAGAGGCTG 
TATCTCCAAA CCCCAACATC GAGTGAGCGG GGAGGCTCTC CAGTTGTGCA GGAACCTGAG 
GAGCCCCCCG AACGCAGGGA GGAGAGTTCA CCTCGGAAAA CCAGCCTCGT GATCGTGGAA 
TCGGCTGAGG ACCAGCCTCA GGCCTTCGAG AGGCTTGATG AAGAGGCTGC TTTTCAAAAG 
GGAGATGATA TGCCTGACAT ACCCCCAGAA ACAGTCACAG AAGAAGAATA CATCGATGAG 
CATGGACACA CCGTGGTAAA GAAGGTTACT AGGAAAATCA TTAGGCGGTT CATCTCCTCT 
GACGGCACAG AGAAAGAGGA GATTACGATG CAGGGAGCAC CACAGGATTC TATCAGCGTT 
GATGAAGGGG ATGGTTATTC CAAAGTCATA AAGCGCATTG TATTGAAGAG TGACACCGAG 
CAGTCAGAGG TAACTTTGTC TGAGCCCAGC ATTCTGTCCA GTACCTCACA ATTTCAGGCT 
GAACCAGTGG AAGGCCGTAG AGTCAGCAAA GTTGTTAAGA CAACTGTGGT CCATGGTGAG 
AGGATGGAGA AACATCTGGG GGATTCTAGT TTAGCCACTG ATCTTCCTTC AGCCAAAGAT 
GACTTTGAAG AGGCTTTGAG TTACACAGGT AGCCACATGA AAGTCTACTC CCCCAGTTTA 
GTAGAGAATG AAGTCCTGAA GGAGGATGGA TCAATAATTA GAAGGACGAC AATGAGTAAA 
GCTAGAACCC AGAAGAGGGC TGTGGTGAAG GACCAGCAGG GAAAACGCAT TTACTTGGAG 
CACCTGGAGG ATGTCCCAGA AGCGCTAGAC CAGGACGACC TCCAGCACGA CCTCCAGCAA 
CTCCTTCGGC ATTTCTGCAA GGAGGACTCG AAGCAAGAGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849258.1
CDS	238..6225
Translation	MGNAALCPACHADKSVRAQGHLQELDRTPDYYGCNGEDTKELGPRSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSAVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHAMAKNGYTPLHIAAKKNQMQIASTLLSYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMTEVLDVSDEEGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSHTLSHASYLRDSAMIDDAVVIPSHQVTTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGFLVSFMVDARGGAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHYCEYTEEELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEIDMTSEKNQQDEQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGRHATDTSLVECLTKINRMDIVHLMETSTEALQERIGHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEHAASKEDEASDHPPIVSEEDISVGYSTLQDGIPKTEGDGSATELLPQTQKEQVQQDFSGKMQDLPEESALEQQEYFVTTPGTEMSETQKATGTSGSPTKTPEEIVTPSEEERLYLQTPTSSERGGSPVVQEPEEPPERREESSPRKTSLVIVESAEDQPQAFERLDEEAAFQKGDDMPDIPPETVTEEEYIDEHGHTVVKKVTRKIIRRFISSDGTEKEEITMQGAPQDSISVDEGDGYSKVIKRIVLKSDTEQSEVTLSEPSILSSTSQFQAEPVEGRRVSKVVKTTVVHGERMEKHLGDSSLATDLPSAKDDFEEALSYTGSHMKVYSPSLVENEVLKEDGSIIRRTTMSKARTQKRAVVKDQQGKRIYLEHLEDVPEALDQDDLQHDLQQLLRHFCKEDSKQEAQ

Target ORF information:

RefSeq Version	XM_024993490.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin 2 (ANK2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993490.1

ORF Insert Sequence:

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
AGTGGATTCA CCCCTTTGCA CATCGCTGCC CACTATGGAA ACGTCAACGT GGCAACTCTC 
CTTCTAAACC GGGGAGCTGC TGTGGACTTC ACAGCCAGGA ATGGAATCAC TCCTCTGCAT 
GTGGCCTCCA AAAGGGGAAA CACAAACATG GTGAAGCTCT TACTGGATCG TGGTGGTCAG 
ATTGATGCCA AAACTAGGGA TGGGTTGACA CCCCTTCATT GTGCTGCAAG AAGCGGTCAT 
GACCAAGTGG TGGAGCTTCT GCTGGAGCGG GGCGCTCCCT TGCTGGCAAG GACGAAGAAC 
GGGCTGTCTC CACTTCACAT GGCTGCACAG GGGGACCACG TGGAATGTGT GAAGCACCTG 
CTCCAGCACA AGGCGCCTGT TGATGACGTC ACTTTGGACT ACTTGACGGC CCTCCACGTT 
GCCGCACACT GCGGCCACTA CCGAGTAACC AAACTTCTTC TGGACAAGAG AGCCAACCCG 
AACGCCAGAG CCCTGAATGG TTTTACTCCA CTGCACATTG CCTGCAAGAA AAACCGCATC 
AAAGTCATGG AACTGCTGGT GAAATATGGG GCTTCAATCC AAGCTATAAC AGAGTCTGGC 
CTCACACCAA TACATGTGGC TGCCTTCATG GGCCACTTGA ACATTGTCCT CCTTCTGCTG 
CAGAACGGAG CCTCTCCAGA TGTCACTAAC ATTCGTGGGG AGACGGCCCT GCACATGGCG 
GCCCGTGCAG GGCAGGTGGA AGTGGTCCGA TGCCTCCTGA GGAACGGTGC CCTCGTCGAT 
GCCAGAGCCA GGGAGGAACA GACGCCTTTG CATATTGCCT CCCGCCTGGG TAAGACAGAA 
ATTGTTCAGC TGCTTCTGCA ACATATGGCT CATCCAGATG CGGCCACTAC AAATGGGTAC 
ACGCCGTTGC ATATCTCTGC AAGGGAAGGA CAGGTGGACG TGGCCTCGGT CCTCCTGGAA 
GCGGGGGCAG CCCACTCCTT GGCTACCAAG AAGGGTTTTA CCCCCTTGCA CGTAGCTGCC 
AAGTATGGGA GCCTGGATGT GGCAAAACTT CTCTTGCAAC GCCGTGCTGC CGCAGATTCT 
GCGGGGAAGA ATGGCCTTAC CCCGCTCCAT GTTGCTGCTC ACTATGACAA CCAGAAGGTG 
GCGCTGCTGT TACTGGAGAA GGGTGCTTCC CCTCACGCCA TGGCCAAGAA TGGCTACACT 
CCTTTACATA TTGCTGCCAA GAAGAATCAA ATGCAGATAG CTTCCACACT CCTGAGCTAC 
GGAGCAGAGA CGAACATCGT GACAAAGCAA GGAGTCACTC CACTCCATCT GGCCTCGCAG 
GAGGGACACA CGGATATGGT CACCTTGCTT CTGGATAAGG GAGCCAATAT CCACATGTCA 
ACCAAGAGTG GACTCACATC TTTACACCTT GCAGCCCAGG AAGATAAAGT GAATGTCGCT 
GATATTCTCA CCAAGCATGG GGCTGACCAG GATGCGCACA CAAAGCTTGG TTACACTCCT 
TTAATTGTGG CCTGTCACTA TGGAAACGTG AAAATGGTCA ACTTCCTTCT GAAGCAGGGA 
GCAAATGTCA ATGCAAAAAC CAAGAATGGC TACACGCCTT TGCACCAAGC CGCCCAACAG 
GGCCACACGC ACATCATCAA TGTTTTGCTG CAGCACGGGG CCAAGCCCAA CGCTACCACC 
GCGAATGGCA ACACTGCCTT GGCGATTGCT AAGCGTCTGG GCTACATCTC GGTGGTGGAC 
ACCCTGAAGG TTGTAACGGA GGAGGTCACC ACCACCACCA CGACTATCAC TGAAAAACAC 
AAGCTAAATG TTCCTGAGAC CATGACTGAG GTTCTTGATG TTTCTGATGA AGAGGGTGAT 
GACACAATGA CGGGTGATGG GGGAGAGTAC CTTAGACCTG AGGACCTAAA GGAGCTGGGT 
GATGACTCCC TCCCCAGCAG TCAGTTCCTG GACGGGATGA ATTACCTGCG CTACAGCTTG 
GAGGGGGGCC GCTCCGACAG CCTTCGATCC TTCAGTTCCG ACAGGTCTCA CACCCTGAGC 
CATGCCTCGT ACCTGAGGGA CAGTGCCATG ATCGACGACG CAGTTGTGAT CCCCAGTCAC 
CAGGTGACAA CTCTAGCCAA GGAGGCAGAA AGGAATTCTT ATCGTCTAAG CTGGGGCACT 
GAGAACTTGG ACAACGTGGC TCTTTCTTCC AGCCCTATTC ATTCAGGTTT CCTGGTTAGT 
TTTATGGTGG ATGCCCGAGG TGGTGCTATG CGAGGATGCA GACACAACGG GCTCCGCATC 
ATTATTCCGC CCCGGAAATG TACCGCCCCA ACACGAGTCA CCTGCCGACT GGTCAAACGC 
CATAGACTGG CGACAATGCC TCCAATGGTG GAAGGAGAGG GCCTGGCCAG TCGCCTGATT 
GAAGTTGGAC CTTCTGGTGC TCAGTTCCTT GGGCCTGTGA TCGTGGAGAT CCCTCATTTC 
GCTGCCCTGC GAGGAAAAGA GAGGGAACTG GTGGTCCTGC GCAGTGAGAA CGGGGACAGC 
TGGAAAGAGC ATTACTGTGA ATACACGGAG GAGGAGCTGA ATGAAATCCT GAACGGCATG 
GATGAAGTGC TGGATAGCCC GGAAGACCTC GAAAAGAAGC GGATCTGCCG CATCATCACC 
CGTGACTTCC CACAGTACTT TGCGGTGGTG TCGCGCATCA AGCAGGACAG CAACCTGATT 
GGCCCGGAGG GAGGCGTGCT GAGCAGCACC GTGGTGCCGC AGGTGCAGGC CGTGTTCCCC 
GAGGGGGCGC TAACCAAGCG GATCCGTGTT GGCCTACAGG CTCAACCTAT GCACAGTGAG 
TTGGTTAAGA AGATTCTGGG CAACAAAGCT ACCTTCAGCC CTATAGTTAC TTTGGAACCT 
AGAAGAAGAA AATTTCACAA GCCAATTACC ATGACCATTC CTGTCCCCAA AGCTTCAAGT 
GATGTCATGT TGAATGGTTT TGGAGGAGAT GCACCAACCT TAAGATTACT ATGTAGCATC 
ACAGGTGGAA CCACCCCTGC TCAGTGGGAA GATATTACAG GAACTACGCC ATTAACATTT 
GTCAATGAAT GTGTTTCCTT TACAACCAAT GTGTCTGCCA GGTTCTGGCT GATCGATTGT 
CGACAAATTC AGGAATCTGT TACCTTTGCA TCACAAGTGT ATAGAGAAAT TATCTGTGTA 
CCATATATGG CCAAATTTGT AGTGTTTGCC AAATCACATG ACCCCATTGA AGCCAGGTTG 
AGGTGTTTCT GCATGACTGA TGATAAAGTG GATAAAACCC TGGAACAACA AGAAAACTTT 
GCTGAGGTGG CCAGAAGCAG GGATGTGGAG GTCTTAGAAG GAAAACCCAT CTATGTTGAT 
TGTTTTGGCA ACCTGGTGCC ATTAACCAAG AGTGGCCAAC ATCATATATT CAGTTTTTTT 
GCCTTCAAAG AAAACAGACT TCCTCTCTTT GTCAAGGTAC GTGATACGAC TCAGGAACCT 
TGCGGACGAC TATCATTTAT GAAAGAGCCA AAATCTACAA GAGGCCTGGT GCACCAAGCT 
ATTTGCAACT TAAACATCAC CCTGCCAATT TATACGAAGG AATCAGAGTC AGATCAAGAA 
CAGGAGGAAG AGATCGATAT GACATCAGAA AAAAATCAAC AGGATGAGCA GGAACGGATT 
GAGGAAAGGC TGGCATATAT TGCTGATCAC CTTGGCTTCA GCTGGACAGA GTTGGCAAGA 
GAACTGGACT TCACTGAAGA GCAAATTCAT CAAATTCGAA TTGAGAACCC TAACTCCCTG 
CAGGATCAGA GTCATGCGCT GTTGAAGTAC TGGCTAGAAA GGGACGGGAG ACATGCTACA 
GATACCAGTC TCGTTGAATG TCTCACCAAG ATCAACCGAA TGGATATCGT GCATCTCATG 
GAGACCAGCA CAGAAGCTCT CCAGGAGCGC ATCGGTCATA GCTATGCAGA GATTGAGCAG 
ACCATTACAC TGGATCATAG TGAAGGGTTC TCAGTACTCC AAGAGGAGCT TTGCACTGCC 
CAGCACAAGC AGAAAGAGGA GCATGCTGCT TCTAAAGAGG ACGAGGCCTC TGACCACCCT 
CCCATCGTCT CAGAGGAAGA CATTTCTGTT GGTTACTCCA CTCTTCAGGA TGGCATCCCC 
AAAACTGAGG GTGATGGCTC TGCAACAGAG CTCCTTCCCC AGACTCAAAA GGAGCAAGTT 
CAACAGGATT TCTCAGGGAA AATGCAAGAC CTGCCTGAGG AGTCGGCTCT TGAACAGCAA 
GAATACTTCG TGACAACCCC AGGGACAGAA ATGTCAGAGA CTCAGAAGGC CACGGGAACA 
TCGGGCTCCC CCACCAAGAC ACCTGAGGAA ATTGTCACCC CTTCCGAGGA GGAGAGGCTG 
TATCTCCAAA CCCCAACATC GAGTGAGCGG GGAGGCTCTC CAGTTGTGCA GGAACCTGAG 
GAGCCCCCCG AACGCAGGGA GGAGAGTTCA CCTCGGAAAA CCAGCCTCGT GATCGTGGAA 
TCGGCTGAGG ACCAGCCTCA GGCCTTCGAG AGGCTTGATG AAGAGGCTGC TTTTCAAAAG 
GGAGATGATA TGCCTGACAT ACCCCCAGAA ACAGTCACAG AAGAAGAATA CATCGATGAG 
CATGGACACA CCGTGGTAAA GAAGGTTACT AGGAAAATCA TTAGGCGGTT CATCTCCTCT 
GACGGCACAG AGAAAGAGGA GATTACGATG CAGGGAGCAC CACAGGATTC TATCAGCGTT 
GATGAAGGGG ATGGTTATTC CAAAGTCATA AAGCGCATTG TATTGAAGAG TGACACCGAG 
CAGTCAGAGG TAACTTTGTC TGAGCCCAGC ATTCTGTCCA GTACCTCACA ATTTCAGGCT 
GAACCAGTGG AAGGCCGTAG AGTCAGCAAA GTTGTTAAGA CAACTGTGGT CCATGGTGAG 
AGGATGGAGA AACATCTGGG GGATTCTAGT TTAGCCACTG ATCTTCCTTC AGCCAAAGAT 
GACTTTGAAG AGGCTTTGAG TTACACAGGT AGCCACATGA AAGTCTACTC CCCCAGTTTA 
GTAGAGAATG AAGTCCTGAA GGAGGATGGA TCAATAATTA GAAGGACGAC AATGAGTAAA 
GCTAGAACCC AGAAGAGGGC TGTGGTGAAG GACCAGCAGG GAAAACGCAT TTACTTGGAG 
CACCTGGAGG ATGTCCCAGA AGCGCTAGAC CAGGACGACC TCCAGCACGA CCTCCAGCAA 
CTCCTTCGGC ATTTCTGCAA GGAGGACTCG AAGCAAGAGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK2 ( XM_024993491.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849259.1 Bos taurus ankyrin 2 (ANK2), transcript variant X7, mRNA.

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CloneID	OBa235483
Clone ID Related Accession (Same CDS sequence)	XM_024993491.1
Accession Version	XM_024993491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin-2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
ATGATGGTGA ATAGGACCAC CGAGAGTGGA TTCACCCCTT TGCACATCGC TGCCCACTAT 
GGAAACGTCA ACGTGGCAAC TCTCCTTCTA AACCGGGGAG CTGCTGTGGA CTTCACAGCC 
AGGAATGGAA TCACTCCTCT GCATGTGGCC TCCAAAAGGG GAAACACAAA CATGGTGAAG 
CTCTTACTGG ATCGTGGTGG TCAGATTGAT GCCAAAACTA GGGATGGGTT GACACCCCTT 
CATTGTGCTG CAAGAAGCGG TCATGACCAA GTGGTGGAGC TTCTGCTGGA GCGGGGCGCT 
CCCTTGCTGG CAAGGACGAA GAACGGGCTG TCTCCACTTC ACATGGCTGC ACAGGGGGAC 
CACGTGGAAT GTGTGAAGCA CCTGCTCCAG CACAAGGCGC CTGTTGATGA CGTCACTTTG 
GACTACTTGA CGGCCCTCCA CGTTGCCGCA CACTGCGGCC ACTACCGAGT AACCAAACTT 
CTTCTGGACA AGAGAGCCAA CCCGAACGCC AGAGCCCTGA ATGGTTTTAC TCCACTGCAC 
ATTGCCTGCA AGAAAAACCG CATCAAAGTC ATGGAACTGC TGGTGAAATA TGGGGCTTCA 
ATCCAAGCTA TAACAGAGTC TGGCCTCACA CCAATACATG TGGCTGCCTT CATGGGCCAC 
TTGAACATTG TCCTCCTTCT GCTGCAGAAC GGAGCCTCTC CAGATGTCAC TAACATTCGT 
GGGGAGACGG CCCTGCACAT GGCGGCCCGT GCAGGGCAGG TGGAAGTGGT CCGATGCCTC 
CTGAGGAACG GTGCCCTCGT CGATGCCAGA GCCAGGGAGG AACAGACGCC TTTGCATATT 
GCCTCCCGCC TGGGTAAGAC AGAAATTGTT CAGCTGCTTC TGCAACATAT GGCTCATCCA 
GATGCGGCCA CTACAAATGG GTACACGCCG TTGCATATCT CTGCAAGGGA AGGACAGGTG 
GACGTGGCCT CGGTCCTCCT GGAAGCGGGG GCAGCCCACT CCTTGGCTAC CAAGAAGGGT 
TTTACCCCCT TGCACGTAGC TGCCAAGTAT GGGAGCCTGG ATGTGGCAAA ACTTCTCTTG 
CAACGCCGTG CTGCCGCAGA TTCTGCGGGG AAGAATGGCT ACACTCCTTT ACATATTGCT 
GCCAAGAAGA ATCAAATGCA GATAGCTTCC ACACTCCTGA GCTACGGAGC AGAGACGAAC 
ATCGTGACAA AGCAAGGAGT CACTCCACTC CATCTGGCCT CGCAGGAGGG ACACACGGAT 
ATGGTCACCT TGCTTCTGGA TAAGGGAGCC AATATCCACA TGTCAACCAA GAGTGGACTC 
ACATCTTTAC ACCTTGCAGC CCAGGAAGAT AAAGTGAATG TCGCTGATAT TCTCACCAAG 
CATGGGGCTG ACCAGGATGC GCACACAAAG CTTGGTTACA CTCCTTTAAT TGTGGCCTGT 
CACTATGGAA ACGTGAAAAT GGTCAACTTC CTTCTGAAGC AGGGAGCAAA TGTCAATGCA 
AAAACCAAGA ATGGCTACAC GCCTTTGCAC CAAGCCGCCC AACAGGGCCA CACGCACATC 
ATCAATGTTT TGCTGCAGCA CGGGGCCAAG CCCAACGCTA CCACCGCGAA TGGCAACACT 
GCCTTGGCGA TTGCTAAGCG TCTGGGCTAC ATCTCGGTGG TGGACACCCT GAAGGTTGTA 
ACGGAGGAGG TCACCACCAC CACCACGACT ATCACTGAAA AACACAAGCT AAATGTTCCT 
GAGACCATGA CTGAGGTTCT TGATGTTTCT GATGAAGAGG GTGATGACAC AATGACGGGT 
GATGGGGGAG AGTACCTTAG ACCTGAGGAC CTAAAGGAGC TGGGTGATGA CTCCCTCCCC 
AGCAGTCAGT TCCTGGACGG GATGAATTAC CTGCGCTACA GCTTGGAGGG GGGCCGCTCC 
GACAGCCTTC GATCCTTCAG TTCCGACAGG TCTCACACCC TGAGCCATGC CTCGTACCTG 
AGGGACAGTG CCATGATCGA CGACGCAGTT GTGATCCCCA GTCACCAGGT GACAACTCTA 
GCCAAGGAGG CAGAAAGGAA TTCTTATCGT CTAAGCTGGG GCACTGAGAA CTTGGACAAC 
GTGGCTCTTT CTTCCAGCCC TATTCATTCA GGCCGCACCT CTCCATGTCT TGATCGTGAC 
AACAGCAGTT TCCTGGTTAG TTTTATGGTG GATGCCCGAG GTGGTGCTAT GCGAGGATGC 
AGACACAACG GGCTCCGCAT CATTATTCCG CCCCGGAAAT GTACCGCCCC AACACGAGTC 
ACCTGCCGAC TGGTCAAACG CCATAGACTG GCGACAATGC CTCCAATGGT GGAAGGAGAG 
GGCCTGGCCA GTCGCCTGAT TGAAGTTGGA CCTTCTGGTG CTCAGTTCCT TGGGCCTGTG 
ATCGTGGAGA TCCCTCATTT CGCTGCCCTG CGAGGAAAAG AGAGGGAACT GGTGGTCCTG 
CGCAGTGAGA ACGGGGACAG CTGGAAAGAG CATTACTGTG AATACACGGA GGAGGAGCTG 
AATGAAATCC TGAACGGCAT GGATGAAGTG CTGGATAGCC CGGAAGACCT CGAAAAGAAG 
CGGATCTGCC GCATCATCAC CCGTGACTTC CCACAGTACT TTGCGGTGGT GTCGCGCATC 
AAGCAGGACA GCAACCTGAT TGGCCCGGAG GGAGGCGTGC TGAGCAGCAC CGTGGTGCCG 
CAGGTGCAGG CCGTGTTCCC CGAGGGGGCG CTAACCAAGC GGATCCGTGT TGGCCTACAG 
GCTCAACCTA TGCACAGTGA GTTGGTTAAG AAGATTCTGG GCAACAAAGC TACCTTCAGC 
CCTATAGTTA CTTTGGAACC TAGAAGAAGA AAATTTCACA AGCCAATTAC CATGACCATT 
CCTGTCCCCA AAGCTTCAAG TGATGTCATG TTGAATGGTT TTGGAGGAGA TGCACCAACC 
TTAAGATTAC TATGTAGCAT CACAGGTGGA ACCACCCCTG CTCAGTGGGA AGATATTACA 
GGAACTACGC CATTAACATT TGTCAATGAA TGTGTTTCCT TTACAACCAA TGTGTCTGCC 
AGGTTCTGGC TGATCGATTG TCGACAAATT CAGGAATCTG TTACCTTTGC ATCACAAGTG 
TATAGAGAAA TTATCTGTGT ACCATATATG GCCAAATTTG TAGTGTTTGC CAAATCACAT 
GACCCCATTG AAGCCAGGTT GAGGTGTTTC TGCATGACTG ATGATAAAGT GGATAAAACC 
CTGGAACAAC AAGAAAACTT TGCTGAGGTG GCCAGAAGCA GGGATGTGGA GGTCTTAGAA 
GGAAAACCCA TCTATGTTGA TTGTTTTGGC AACCTGGTGC CATTAACCAA GAGTGGCCAA 
CATCATATAT TCAGTTTTTT TGCCTTCAAA GAAAACAGAC TTCCTCTCTT TGTCAAGGTA 
CGTGATACGA CTCAGGAACC TTGCGGACGA CTATCATTTA TGAAAGAGCC AAAATCTACA 
AGAGGCCTGG TGCACCAAGC TATTTGCAAC TTAAACATCA CCCTGCCAAT TTATACGAAG 
GAATCAGAGT CAGATCAAGA ACAGGAGGAA GAGATCGATA TGACATCAGA AAAAAATCAA 
CAGGATGAGC AGGAACGGAT TGAGGAAAGG CTGGCATATA TTGCTGATCA CCTTGGCTTC 
AGCTGGACAG AGTTGGCAAG AGAACTGGAC TTCACTGAAG AGCAAATTCA TCAAATTCGA 
ATTGAGAACC CTAACTCCCT GCAGGATCAG AGTCATGCGC TGTTGAAGTA CTGGCTAGAA 
AGGGACGGGA GACATGCTAC AGATACCAGT CTCGTTGAAT GTCTCACCAA GATCAACCGA 
ATGGATATCG TGCATCTCAT GGAGACCAGC ACAGAAGCTC TCCAGGAGCG CATCGGTCAT 
AGCTATGCAG AGATTGAGCA GACCATTACA CTGGATCATA GTGAAGGGTT CTCAGTACTC 
CAAGAGGAGC TTTGCACTGC CCAGCACAAG CAGAAAGAGG AGCATGCTGC TTCTAAAGAG 
GACGAGGCCT CTGACCACCC TCCCATCGTC TCAGAGGAAG ACATTTCTGT TGGTTACTCC 
ACTCTTCAGG ATGGCATCCC CAAAACTGAG GGTGATGGCT CTGCAACAGA GCTCCTTCCC 
CAGACTCAAA AGGAGCAAGT TCAACAGGAT TTCTCAGGGA AAATGCAAGA CCTGCCTGAG 
GAGTCGGCTC TTGAACAGCA AGAATACTTC GTGACAACCC CAGGGACAGA AATGTCAGAG 
ACTCAGAAGG CCACGGGAAC ATCGGGCTCC CCCACCAAGA CACCTGAGGA AATTGTCACC 
CCTTCCGAGG AGGAGAGGCT GTATCTCCAA ACCCCAACAT CGAGTGAGCG GGGAGGCTCT 
CCAGTTGTGC AGGAACCTGA GGAGCCCCCC GAACGCAGGG AGGAGAGTTC ACCTCGGAAA 
ACCAGCCTCG TGATCGTGGA ATCGGCTGAG GACCAGCCTC AGGCCTTCGA GAGGCTTGAT 
GAAGAGGCTG CTTTTCAAAA GGGAGATGAT ATGCCTGACA TACCCCCAGA AACAGTCACA 
GAAGAAGAAT ACATCGATGA GCATGGACAC ACCGTGGTAA AGAAGGTTAC TAGGAAAATC 
ATTAGGCGGT TCATCTCCTC TGACGGCACA GAGAAAGAGG AGATTACGAT GCAGGGAGCA 
CCACAGGATT CTATCAGCGT TGATGAAGGG GATGGTTATT CCAAAGTCAT AAAGCGCATT 
GTATTGAAGA GTGACACCGA GCAGTCAGAG GTAACTTTGT CTGAGCCCAG CATTCTGTCC 
AGTACCTCAC AATTTCAGGC TGAACCAGTG GAAGGCCGTA GAGTCAGCAA AGTTGTTAAG 
ACAACTGTGG TCCATGGTGA GAGGATGGAG AAACATCTGG GGGATTCTAG TTTAGCCACT 
GATCTTCCTT CAGCCAAAGA TGACTTTGAA GAGGCTTTGA GTTACACAGG TAGCCACATG 
AAAGTCTACT CCCCCAGTTT AGTAGAGAAT GAAGTCCTGA AGGAGGATGG ATCAATAATT 
AGAAGGACGA CAATGAGTAA AGCTAGAACC CAGAAGAGGG CTGTGGTGAA GGACCAGCAG 
GGAAAACGCA TTTACTTGGA GCACCTGGAG GATGTCCCAG AAGCGCTAGA CCAGGACGAC 
CTCCAGCACG ACCTCCAGCA ACTCCTTCGG CATTTCTGCA AGGAGGACTC GAAGCAAGAG 
GCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849259.1
CDS	239..6187
Translation	MGNAALCPACHADKSVRAQGHLQELDRTPDYYGCNGEDTKELGPRSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSAVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQIASTLLSYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMTEVLDVSDEEGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSHTLSHASYLRDSAMIDDAVVIPSHQVTTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGRTSPCLDRDNSSFLVSFMVDARGGAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHYCEYTEEELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEIDMTSEKNQQDEQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGRHATDTSLVECLTKINRMDIVHLMETSTEALQERIGHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEHAASKEDEASDHPPIVSEEDISVGYSTLQDGIPKTEGDGSATELLPQTQKEQVQQDFSGKMQDLPEESALEQQEYFVTTPGTEMSETQKATGTSGSPTKTPEEIVTPSEEERLYLQTPTSSERGGSPVVQEPEEPPERREESSPRKTSLVIVESAEDQPQAFERLDEEAAFQKGDDMPDIPPETVTEEEYIDEHGHTVVKKVTRKIIRRFISSDGTEKEEITMQGAPQDSISVDEGDGYSKVIKRIVLKSDTEQSEVTLSEPSILSSTSQFQAEPVEGRRVSKVVKTTVVHGERMEKHLGDSSLATDLPSAKDDFEEALSYTGSHMKVYSPSLVENEVLKEDGSIIRRTTMSKARTQKRAVVKDQQGKRIYLEHLEDVPEALDQDDLQHDLQQLLRHFCKEDSKQEAQ

Target ORF information:

RefSeq Version	XM_024993491.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin 2 (ANK2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993491.1

ORF Insert Sequence:

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
ATGATGGTGA ATAGGACCAC CGAGAGTGGA TTCACCCCTT TGCACATCGC TGCCCACTAT 
GGAAACGTCA ACGTGGCAAC TCTCCTTCTA AACCGGGGAG CTGCTGTGGA CTTCACAGCC 
AGGAATGGAA TCACTCCTCT GCATGTGGCC TCCAAAAGGG GAAACACAAA CATGGTGAAG 
CTCTTACTGG ATCGTGGTGG TCAGATTGAT GCCAAAACTA GGGATGGGTT GACACCCCTT 
CATTGTGCTG CAAGAAGCGG TCATGACCAA GTGGTGGAGC TTCTGCTGGA GCGGGGCGCT 
CCCTTGCTGG CAAGGACGAA GAACGGGCTG TCTCCACTTC ACATGGCTGC ACAGGGGGAC 
CACGTGGAAT GTGTGAAGCA CCTGCTCCAG CACAAGGCGC CTGTTGATGA CGTCACTTTG 
GACTACTTGA CGGCCCTCCA CGTTGCCGCA CACTGCGGCC ACTACCGAGT AACCAAACTT 
CTTCTGGACA AGAGAGCCAA CCCGAACGCC AGAGCCCTGA ATGGTTTTAC TCCACTGCAC 
ATTGCCTGCA AGAAAAACCG CATCAAAGTC ATGGAACTGC TGGTGAAATA TGGGGCTTCA 
ATCCAAGCTA TAACAGAGTC TGGCCTCACA CCAATACATG TGGCTGCCTT CATGGGCCAC 
TTGAACATTG TCCTCCTTCT GCTGCAGAAC GGAGCCTCTC CAGATGTCAC TAACATTCGT 
GGGGAGACGG CCCTGCACAT GGCGGCCCGT GCAGGGCAGG TGGAAGTGGT CCGATGCCTC 
CTGAGGAACG GTGCCCTCGT CGATGCCAGA GCCAGGGAGG AACAGACGCC TTTGCATATT 
GCCTCCCGCC TGGGTAAGAC AGAAATTGTT CAGCTGCTTC TGCAACATAT GGCTCATCCA 
GATGCGGCCA CTACAAATGG GTACACGCCG TTGCATATCT CTGCAAGGGA AGGACAGGTG 
GACGTGGCCT CGGTCCTCCT GGAAGCGGGG GCAGCCCACT CCTTGGCTAC CAAGAAGGGT 
TTTACCCCCT TGCACGTAGC TGCCAAGTAT GGGAGCCTGG ATGTGGCAAA ACTTCTCTTG 
CAACGCCGTG CTGCCGCAGA TTCTGCGGGG AAGAATGGCT ACACTCCTTT ACATATTGCT 
GCCAAGAAGA ATCAAATGCA GATAGCTTCC ACACTCCTGA GCTACGGAGC AGAGACGAAC 
ATCGTGACAA AGCAAGGAGT CACTCCACTC CATCTGGCCT CGCAGGAGGG ACACACGGAT 
ATGGTCACCT TGCTTCTGGA TAAGGGAGCC AATATCCACA TGTCAACCAA GAGTGGACTC 
ACATCTTTAC ACCTTGCAGC CCAGGAAGAT AAAGTGAATG TCGCTGATAT TCTCACCAAG 
CATGGGGCTG ACCAGGATGC GCACACAAAG CTTGGTTACA CTCCTTTAAT TGTGGCCTGT 
CACTATGGAA ACGTGAAAAT GGTCAACTTC CTTCTGAAGC AGGGAGCAAA TGTCAATGCA 
AAAACCAAGA ATGGCTACAC GCCTTTGCAC CAAGCCGCCC AACAGGGCCA CACGCACATC 
ATCAATGTTT TGCTGCAGCA CGGGGCCAAG CCCAACGCTA CCACCGCGAA TGGCAACACT 
GCCTTGGCGA TTGCTAAGCG TCTGGGCTAC ATCTCGGTGG TGGACACCCT GAAGGTTGTA 
ACGGAGGAGG TCACCACCAC CACCACGACT ATCACTGAAA AACACAAGCT AAATGTTCCT 
GAGACCATGA CTGAGGTTCT TGATGTTTCT GATGAAGAGG GTGATGACAC AATGACGGGT 
GATGGGGGAG AGTACCTTAG ACCTGAGGAC CTAAAGGAGC TGGGTGATGA CTCCCTCCCC 
AGCAGTCAGT TCCTGGACGG GATGAATTAC CTGCGCTACA GCTTGGAGGG GGGCCGCTCC 
GACAGCCTTC GATCCTTCAG TTCCGACAGG TCTCACACCC TGAGCCATGC CTCGTACCTG 
AGGGACAGTG CCATGATCGA CGACGCAGTT GTGATCCCCA GTCACCAGGT GACAACTCTA 
GCCAAGGAGG CAGAAAGGAA TTCTTATCGT CTAAGCTGGG GCACTGAGAA CTTGGACAAC 
GTGGCTCTTT CTTCCAGCCC TATTCATTCA GGCCGCACCT CTCCATGTCT TGATCGTGAC 
AACAGCAGTT TCCTGGTTAG TTTTATGGTG GATGCCCGAG GTGGTGCTAT GCGAGGATGC 
AGACACAACG GGCTCCGCAT CATTATTCCG CCCCGGAAAT GTACCGCCCC AACACGAGTC 
ACCTGCCGAC TGGTCAAACG CCATAGACTG GCGACAATGC CTCCAATGGT GGAAGGAGAG 
GGCCTGGCCA GTCGCCTGAT TGAAGTTGGA CCTTCTGGTG CTCAGTTCCT TGGGCCTGTG 
ATCGTGGAGA TCCCTCATTT CGCTGCCCTG CGAGGAAAAG AGAGGGAACT GGTGGTCCTG 
CGCAGTGAGA ACGGGGACAG CTGGAAAGAG CATTACTGTG AATACACGGA GGAGGAGCTG 
AATGAAATCC TGAACGGCAT GGATGAAGTG CTGGATAGCC CGGAAGACCT CGAAAAGAAG 
CGGATCTGCC GCATCATCAC CCGTGACTTC CCACAGTACT TTGCGGTGGT GTCGCGCATC 
AAGCAGGACA GCAACCTGAT TGGCCCGGAG GGAGGCGTGC TGAGCAGCAC CGTGGTGCCG 
CAGGTGCAGG CCGTGTTCCC CGAGGGGGCG CTAACCAAGC GGATCCGTGT TGGCCTACAG 
GCTCAACCTA TGCACAGTGA GTTGGTTAAG AAGATTCTGG GCAACAAAGC TACCTTCAGC 
CCTATAGTTA CTTTGGAACC TAGAAGAAGA AAATTTCACA AGCCAATTAC CATGACCATT 
CCTGTCCCCA AAGCTTCAAG TGATGTCATG TTGAATGGTT TTGGAGGAGA TGCACCAACC 
TTAAGATTAC TATGTAGCAT CACAGGTGGA ACCACCCCTG CTCAGTGGGA AGATATTACA 
GGAACTACGC CATTAACATT TGTCAATGAA TGTGTTTCCT TTACAACCAA TGTGTCTGCC 
AGGTTCTGGC TGATCGATTG TCGACAAATT CAGGAATCTG TTACCTTTGC ATCACAAGTG 
TATAGAGAAA TTATCTGTGT ACCATATATG GCCAAATTTG TAGTGTTTGC CAAATCACAT 
GACCCCATTG AAGCCAGGTT GAGGTGTTTC TGCATGACTG ATGATAAAGT GGATAAAACC 
CTGGAACAAC AAGAAAACTT TGCTGAGGTG GCCAGAAGCA GGGATGTGGA GGTCTTAGAA 
GGAAAACCCA TCTATGTTGA TTGTTTTGGC AACCTGGTGC CATTAACCAA GAGTGGCCAA 
CATCATATAT TCAGTTTTTT TGCCTTCAAA GAAAACAGAC TTCCTCTCTT TGTCAAGGTA 
CGTGATACGA CTCAGGAACC TTGCGGACGA CTATCATTTA TGAAAGAGCC AAAATCTACA 
AGAGGCCTGG TGCACCAAGC TATTTGCAAC TTAAACATCA CCCTGCCAAT TTATACGAAG 
GAATCAGAGT CAGATCAAGA ACAGGAGGAA GAGATCGATA TGACATCAGA AAAAAATCAA 
CAGGATGAGC AGGAACGGAT TGAGGAAAGG CTGGCATATA TTGCTGATCA CCTTGGCTTC 
AGCTGGACAG AGTTGGCAAG AGAACTGGAC TTCACTGAAG AGCAAATTCA TCAAATTCGA 
ATTGAGAACC CTAACTCCCT GCAGGATCAG AGTCATGCGC TGTTGAAGTA CTGGCTAGAA 
AGGGACGGGA GACATGCTAC AGATACCAGT CTCGTTGAAT GTCTCACCAA GATCAACCGA 
ATGGATATCG TGCATCTCAT GGAGACCAGC ACAGAAGCTC TCCAGGAGCG CATCGGTCAT 
AGCTATGCAG AGATTGAGCA GACCATTACA CTGGATCATA GTGAAGGGTT CTCAGTACTC 
CAAGAGGAGC TTTGCACTGC CCAGCACAAG CAGAAAGAGG AGCATGCTGC TTCTAAAGAG 
GACGAGGCCT CTGACCACCC TCCCATCGTC TCAGAGGAAG ACATTTCTGT TGGTTACTCC 
ACTCTTCAGG ATGGCATCCC CAAAACTGAG GGTGATGGCT CTGCAACAGA GCTCCTTCCC 
CAGACTCAAA AGGAGCAAGT TCAACAGGAT TTCTCAGGGA AAATGCAAGA CCTGCCTGAG 
GAGTCGGCTC TTGAACAGCA AGAATACTTC GTGACAACCC CAGGGACAGA AATGTCAGAG 
ACTCAGAAGG CCACGGGAAC ATCGGGCTCC CCCACCAAGA CACCTGAGGA AATTGTCACC 
CCTTCCGAGG AGGAGAGGCT GTATCTCCAA ACCCCAACAT CGAGTGAGCG GGGAGGCTCT 
CCAGTTGTGC AGGAACCTGA GGAGCCCCCC GAACGCAGGG AGGAGAGTTC ACCTCGGAAA 
ACCAGCCTCG TGATCGTGGA ATCGGCTGAG GACCAGCCTC AGGCCTTCGA GAGGCTTGAT 
GAAGAGGCTG CTTTTCAAAA GGGAGATGAT ATGCCTGACA TACCCCCAGA AACAGTCACA 
GAAGAAGAAT ACATCGATGA GCATGGACAC ACCGTGGTAA AGAAGGTTAC TAGGAAAATC 
ATTAGGCGGT TCATCTCCTC TGACGGCACA GAGAAAGAGG AGATTACGAT GCAGGGAGCA 
CCACAGGATT CTATCAGCGT TGATGAAGGG GATGGTTATT CCAAAGTCAT AAAGCGCATT 
GTATTGAAGA GTGACACCGA GCAGTCAGAG GTAACTTTGT CTGAGCCCAG CATTCTGTCC 
AGTACCTCAC AATTTCAGGC TGAACCAGTG GAAGGCCGTA GAGTCAGCAA AGTTGTTAAG 
ACAACTGTGG TCCATGGTGA GAGGATGGAG AAACATCTGG GGGATTCTAG TTTAGCCACT 
GATCTTCCTT CAGCCAAAGA TGACTTTGAA GAGGCTTTGA GTTACACAGG TAGCCACATG 
AAAGTCTACT CCCCCAGTTT AGTAGAGAAT GAAGTCCTGA AGGAGGATGG ATCAATAATT 
AGAAGGACGA CAATGAGTAA AGCTAGAACC CAGAAGAGGG CTGTGGTGAA GGACCAGCAG 
GGAAAACGCA TTTACTTGGA GCACCTGGAG GATGTCCCAG AAGCGCTAGA CCAGGACGAC 
CTCCAGCACG ACCTCCAGCA ACTCCTTCGG CATTTCTGCA AGGAGGACTC GAAGCAAGAG 
GCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK2 ( XM_024993492.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849260.1 Bos taurus ankyrin 2 (ANK2), transcript variant X8, mRNA.

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CloneID	OBa235484
Clone ID Related Accession (Same CDS sequence)	XM_024993492.1
Accession Version	XM_024993492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin-2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
ATGATGGTGA ATAGGACCAC CGAGAGTGGA TTCACCCCTT TGCACATCGC TGCCCACTAT 
GGAAACGTCA ACGTGGCAAC TCTCCTTCTA AACCGGGGAG CTGCTGTGGA CTTCACAGCC 
AGGAATGGAA TCACTCCTCT GCATGTGGCC TCCAAAAGGG GAAACACAAA CATGGTGAAG 
CTCTTACTGG ATCGTGGTGG TCAGATTGAT GCCAAAACTA GGGATGGGTT GACACCCCTT 
CATTGTGCTG CAAGAAGCGG TCATGACCAA GTGGTGGAGC TTCTGCTGGA GCGGGGCGCT 
CCCTTGCTGG CAAGGACGAA GAACGGGCTG TCTCCACTTC ACATGGCTGC ACAGGGGGAC 
CACGTGGAAT GTGTGAAGCA CCTGCTCCAG CACAAGGCGC CTGTTGATGA CGTCACTTTG 
GACTACTTGA CGGCCCTCCA CGTTGCCGCA CACTGCGGCC ACTACCGAGT AACCAAACTT 
CTTCTGGACA AGAGAGCCAA CCCGAACGCC AGAGCCCTGA ATGGTTTTAC TCCACTGCAC 
ATTGCCTGCA AGAAAAACCG CATCAAAGTC ATGGAACTGC TGGTGAAATA TGGGGCTTCA 
ATCCAAGCTA TAACAGAGTC TGGCCTCACA CCAATACATG TGGCTGCCTT CATGGGCCAC 
TTGAACATTG TCCTCCTTCT GCTGCAGAAC GGAGCCTCTC CAGATGTCAC TAACATTCGT 
GGGGAGACGG CCCTGCACAT GGCGGCCCGT GCAGGGCAGG TGGAAGTGGT CCGATGCCTC 
CTGAGGAACG GTGCCCTCGT CGATGCCAGA GCCAGGGAGG AACAGACGCC TTTGCATATT 
GCCTCCCGCC TGGGTAAGAC AGAAATTGTT CAGCTGCTTC TGCAACATAT GGCTCATCCA 
GATGCGGCCA CTACAAATGG GTACACGCCG TTGCATATCT CTGCAAGGGA AGGACAGGTG 
GACGTGGCCT CGGTCCTCCT GGAAGCGGGG GCAGCCCACT CCTTGGCTAC CAAGAAGGGT 
TTTACCCCCT TGCACGTAGC TGCCAAGTAT GGGAGCCTGG ATGTGGCAAA ACTTCTCTTG 
CAACGCCGTG CTGCCGCAGA TTCTGCGGGG AAGAATGGCT ACACTCCTTT ACATATTGCT 
GCCAAGAAGA ATCAAATGCA GATAGCTTCC ACACTCCTGA GCTACGGAGC AGAGACGAAC 
ATCGTGACAA AGCAAGGAGT CACTCCACTC CATCTGGCCT CGCAGGAGGG ACACACGGAT 
ATGGTCACCT TGCTTCTGGA TAAGGGAGCC AATATCCACA TGTCAACCAA GAGTGGACTC 
ACATCTTTAC ACCTTGCAGC CCAGGAAGAT AAAGTGAATG TCGCTGATAT TCTCACCAAG 
CATGGGGCTG ACCAGGATGC GCACACAAAG CTTGGTTACA CTCCTTTAAT TGTGGCCTGT 
CACTATGGAA ACGTGAAAAT GGTCAACTTC CTTCTGAAGC AGGGAGCAAA TGTCAATGCA 
AAAACCAAGA ATGGCTACAC GCCTTTGCAC CAAGCCGCCC AACAGGGCCA CACGCACATC 
ATCAATGTTT TGCTGCAGCA CGGGGCCAAG CCCAACGCTA CCACCGCGAA TGGCAACACT 
GCCTTGGCGA TTGCTAAGCG TCTGGGCTAC ATCTCGGTGG TGGACACCCT GAAGGTTGTA 
ACGGAGGAGG TCACCACCAC CACCACGACT ATCACTGAAA AACACAAGCT AAATGTTCCT 
GAGACCATGA CTGAGGTTCT TGATGTTTCT GATGAAGAGG GTGATGACAC AATGACGGGT 
GATGGGGGAG AGTACCTTAG ACCTGAGGAC CTAAAGGAGC TGGGTGATGA CTCCCTCCCC 
AGCAGTCAGT TCCTGGACGG GATGAATTAC CTGCGCTACA GCTTGGAGGG GGGCCGCTCC 
GACAGCCTTC GATCCTTCAG TTCCGACAGG TCTCACACCC TGAGCCATGC CTCGTACCTG 
AGGGACAGTG CCATGATCGA CGACGCAGTT GTGATCCCCA GTCACCAGGT GACAACTCTA 
GCCAAGGAGG CAGAAAGGAA TTCTTATCGT CTAAGCTGGG GCACTGAGAA CTTGGACAAC 
GTGGCTCTTT CTTCCAGCCC TATTCATTCA GGTTTCCTGG TTAGTTTTAT GGTGGATGCC 
CGAGGTGGTG CTATGCGAGG ATGCAGACAC AACGGGCTCC GCATCATTAT TCCGCCCCGG 
AAATGTACCG CCCCAACACG AGTCACCTGC CGACTGGTCA AACGCCATAG ACTGGCGACA 
ATGCCTCCAA TGGTGGAAGG AGAGGGCCTG GCCAGTCGCC TGATTGAAGT TGGACCTTCT 
GGTGCTCAGT TCCTTGGGCC TGTGATCGTG GAGATCCCTC ATTTCGCTGC CCTGCGAGGA 
AAAGAGAGGG AACTGGTGGT CCTGCGCAGT GAGAACGGGG ACAGCTGGAA AGAGCATTAC 
TGTGAATACA CGGAGGAGGA GCTGAATGAA ATCCTGAACG GCATGGATGA AGTGCTGGAT 
AGCCCGGAAG ACCTCGAAAA GAAGCGGATC TGCCGCATCA TCACCCGTGA CTTCCCACAG 
TACTTTGCGG TGGTGTCGCG CATCAAGCAG GACAGCAACC TGATTGGCCC GGAGGGAGGC 
GTGCTGAGCA GCACCGTGGT GCCGCAGGTG CAGGCCGTGT TCCCCGAGGG GGCGCTAACC 
AAGCGGATCC GTGTTGGCCT ACAGGCTCAA CCTATGCACA GTGAGTTGGT TAAGAAGATT 
CTGGGCAACA AAGCTACCTT CAGCCCTATA GTTACTTTGG AACCTAGAAG AAGAAAATTT 
CACAAGCCAA TTACCATGAC CATTCCTGTC CCCAAAGCTT CAAGTGATGT CATGTTGAAT 
GGTTTTGGAG GAGATGCACC AACCTTAAGA TTACTATGTA GCATCACAGG TGGAACCACC 
CCTGCTCAGT GGGAAGATAT TACAGGAACT ACGCCATTAA CATTTGTCAA TGAATGTGTT 
TCCTTTACAA CCAATGTGTC TGCCAGGTTC TGGCTGATCG ATTGTCGACA AATTCAGGAA 
TCTGTTACCT TTGCATCACA AGTGTATAGA GAAATTATCT GTGTACCATA TATGGCCAAA 
TTTGTAGTGT TTGCCAAATC ACATGACCCC ATTGAAGCCA GGTTGAGGTG TTTCTGCATG 
ACTGATGATA AAGTGGATAA AACCCTGGAA CAACAAGAAA ACTTTGCTGA GGTGGCCAGA 
AGCAGGGATG TGGAGGTCTT AGAAGGAAAA CCCATCTATG TTGATTGTTT TGGCAACCTG 
GTGCCATTAA CCAAGAGTGG CCAACATCAT ATATTCAGTT TTTTTGCCTT CAAAGAAAAC 
AGACTTCCTC TCTTTGTCAA GGTACGTGAT ACGACTCAGG AACCTTGCGG ACGACTATCA 
TTTATGAAAG AGCCAAAATC TACAAGAGGC CTGGTGCACC AAGCTATTTG CAACTTAAAC 
ATCACCCTGC CAATTTATAC GAAGGAATCA GAGTCAGATC AAGAACAGGA GGAAGAGATC 
GATATGACAT CAGAAAAAAA TCAACAGGAT GAGCAGGAAC GGATTGAGGA AAGGCTGGCA 
TATATTGCTG ATCACCTTGG CTTCAGCTGG ACAGAGTTGG CAAGAGAACT GGACTTCACT 
GAAGAGCAAA TTCATCAAAT TCGAATTGAG AACCCTAACT CCCTGCAGGA TCAGAGTCAT 
GCGCTGTTGA AGTACTGGCT AGAAAGGGAC GGGAGACATG CTACAGATAC CAGTCTCGTT 
GAATGTCTCA CCAAGATCAA CCGAATGGAT ATCGTGCATC TCATGGAGAC CAGCACAGAA 
GCTCTCCAGG AGCGCATCGG TCATAGCTAT GCAGAGATTG AGCAGACCAT TACACTGGAT 
CATAGTGAAG GGTTCTCAGT ACTCCAAGAG GAGCTTTGCA CTGCCCAGCA CAAGCAGAAA 
GAGGAGCATG CTGCTTCTAA AGAGGACGAG GCCTCTGACC ACCCTCCCAT CGTCTCAGAG 
GAAGACATTT CTGTTGGTTA CTCCACTCTT CAGGATGGCA TCCCCAAAAC TGAGGGTGAT 
GGCTCTGCAA CAGAGCTCCT TCCCCAGACT CAAAAGGAGC AAGTTCAACA GGATTTCTCA 
GGGAAAATGC AAGACCTGCC TGAGGAGTCG GCTCTTGAAC AGCAAGAATA CTTCGTGACA 
ACCCCAGGGA CAGAAATGTC AGAGACTCAG AAGGCCACGG GAACATCGGG CTCCCCCACC 
AAGACACCTG AGGAAATTGT CACCCCTTCC GAGGAGGAGA GGCTGTATCT CCAAACCCCA 
ACATCGAGTG AGCGGGGAGG CTCTCCAGTT GTGCAGGAAC CTGAGGAGCC CCCCGAACGC 
AGGGAGGAGA GTTCACCTCG GAAAACCAGC CTCGTGATCG TGGAATCGGC TGAGGACCAG 
CCTCAGGCCT TCGAGAGGCT TGATGAAGAG GCTGCTTTTC AAAAGGGAGA TGATATGCCT 
GACATACCCC CAGAAACAGT CACAGAAGAA GAATACATCG ATGAGCATGG ACACACCGTG 
GTAAAGAAGG TTACTAGGAA AATCATTAGG CGGTTCATCT CCTCTGACGG CACAGAGAAA 
GAGGAGATTA CGATGCAGGG AGCACCACAG GATTCTATCA GCGTTGATGA AGGGGATGGT 
TATTCCAAAG TCATAAAGCG CATTGTATTG AAGAGTGACA CCGAGCAGTC AGAGGTAACT 
TTGTCTGAGC CCAGCATTCT GTCCAGTACC TCACAATTTC AGGCTGAACC AGTGGAAGGC 
CGTAGAGTCA GCAAAGTTGT TAAGACAACT GTGGTCCATG GTGAGAGGAT GGAGAAACAT 
CTGGGGGATT CTAGTTTAGC CACTGATCTT CCTTCAGCCA AAGATGACTT TGAAGAGGCT 
TTGAGTTACA CAGGTAGCCA CATGAAAGTC TACTCCCCCA GTTTAGTAGA GAATGAAGTC 
CTGAAGGAGG ATGGATCAAT AATTAGAAGG ACGACAATGA GTAAAGCTAG AACCCAGAAG 
AGGGCTGTGG TGAAGGACCA GCAGGGAAAA CGCATTTACT TGGAGCACCT GGAGGATGTC 
CCAGAAGCGC TAGACCAGGA CGACCTCCAG CACGACCTCC AGCAACTCCT TCGGCATTTC 
TGCAAGGAGG ACTCGAAGCA AGAGGCCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849260.1
CDS	238..6150
Translation	MGNAALCPACHADKSVRAQGHLQELDRTPDYYGCNGEDTKELGPRSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSAVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQIASTLLSYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMTEVLDVSDEEGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSHTLSHASYLRDSAMIDDAVVIPSHQVTTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGFLVSFMVDARGGAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHYCEYTEEELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEIDMTSEKNQQDEQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGRHATDTSLVECLTKINRMDIVHLMETSTEALQERIGHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEHAASKEDEASDHPPIVSEEDISVGYSTLQDGIPKTEGDGSATELLPQTQKEQVQQDFSGKMQDLPEESALEQQEYFVTTPGTEMSETQKATGTSGSPTKTPEEIVTPSEEERLYLQTPTSSERGGSPVVQEPEEPPERREESSPRKTSLVIVESAEDQPQAFERLDEEAAFQKGDDMPDIPPETVTEEEYIDEHGHTVVKKVTRKIIRRFISSDGTEKEEITMQGAPQDSISVDEGDGYSKVIKRIVLKSDTEQSEVTLSEPSILSSTSQFQAEPVEGRRVSKVVKTTVVHGERMEKHLGDSSLATDLPSAKDDFEEALSYTGSHMKVYSPSLVENEVLKEDGSIIRRTTMSKARTQKRAVVKDQQGKRIYLEHLEDVPEALDQDDLQHDLQQLLRHFCKEDSKQEAQ

Target ORF information:

RefSeq Version	XM_024993492.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin 2 (ANK2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993492.1

ORF Insert Sequence:

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
ATGATGGTGA ATAGGACCAC CGAGAGTGGA TTCACCCCTT TGCACATCGC TGCCCACTAT 
GGAAACGTCA ACGTGGCAAC TCTCCTTCTA AACCGGGGAG CTGCTGTGGA CTTCACAGCC 
AGGAATGGAA TCACTCCTCT GCATGTGGCC TCCAAAAGGG GAAACACAAA CATGGTGAAG 
CTCTTACTGG ATCGTGGTGG TCAGATTGAT GCCAAAACTA GGGATGGGTT GACACCCCTT 
CATTGTGCTG CAAGAAGCGG TCATGACCAA GTGGTGGAGC TTCTGCTGGA GCGGGGCGCT 
CCCTTGCTGG CAAGGACGAA GAACGGGCTG TCTCCACTTC ACATGGCTGC ACAGGGGGAC 
CACGTGGAAT GTGTGAAGCA CCTGCTCCAG CACAAGGCGC CTGTTGATGA CGTCACTTTG 
GACTACTTGA CGGCCCTCCA CGTTGCCGCA CACTGCGGCC ACTACCGAGT AACCAAACTT 
CTTCTGGACA AGAGAGCCAA CCCGAACGCC AGAGCCCTGA ATGGTTTTAC TCCACTGCAC 
ATTGCCTGCA AGAAAAACCG CATCAAAGTC ATGGAACTGC TGGTGAAATA TGGGGCTTCA 
ATCCAAGCTA TAACAGAGTC TGGCCTCACA CCAATACATG TGGCTGCCTT CATGGGCCAC 
TTGAACATTG TCCTCCTTCT GCTGCAGAAC GGAGCCTCTC CAGATGTCAC TAACATTCGT 
GGGGAGACGG CCCTGCACAT GGCGGCCCGT GCAGGGCAGG TGGAAGTGGT CCGATGCCTC 
CTGAGGAACG GTGCCCTCGT CGATGCCAGA GCCAGGGAGG AACAGACGCC TTTGCATATT 
GCCTCCCGCC TGGGTAAGAC AGAAATTGTT CAGCTGCTTC TGCAACATAT GGCTCATCCA 
GATGCGGCCA CTACAAATGG GTACACGCCG TTGCATATCT CTGCAAGGGA AGGACAGGTG 
GACGTGGCCT CGGTCCTCCT GGAAGCGGGG GCAGCCCACT CCTTGGCTAC CAAGAAGGGT 
TTTACCCCCT TGCACGTAGC TGCCAAGTAT GGGAGCCTGG ATGTGGCAAA ACTTCTCTTG 
CAACGCCGTG CTGCCGCAGA TTCTGCGGGG AAGAATGGCT ACACTCCTTT ACATATTGCT 
GCCAAGAAGA ATCAAATGCA GATAGCTTCC ACACTCCTGA GCTACGGAGC AGAGACGAAC 
ATCGTGACAA AGCAAGGAGT CACTCCACTC CATCTGGCCT CGCAGGAGGG ACACACGGAT 
ATGGTCACCT TGCTTCTGGA TAAGGGAGCC AATATCCACA TGTCAACCAA GAGTGGACTC 
ACATCTTTAC ACCTTGCAGC CCAGGAAGAT AAAGTGAATG TCGCTGATAT TCTCACCAAG 
CATGGGGCTG ACCAGGATGC GCACACAAAG CTTGGTTACA CTCCTTTAAT TGTGGCCTGT 
CACTATGGAA ACGTGAAAAT GGTCAACTTC CTTCTGAAGC AGGGAGCAAA TGTCAATGCA 
AAAACCAAGA ATGGCTACAC GCCTTTGCAC CAAGCCGCCC AACAGGGCCA CACGCACATC 
ATCAATGTTT TGCTGCAGCA CGGGGCCAAG CCCAACGCTA CCACCGCGAA TGGCAACACT 
GCCTTGGCGA TTGCTAAGCG TCTGGGCTAC ATCTCGGTGG TGGACACCCT GAAGGTTGTA 
ACGGAGGAGG TCACCACCAC CACCACGACT ATCACTGAAA AACACAAGCT AAATGTTCCT 
GAGACCATGA CTGAGGTTCT TGATGTTTCT GATGAAGAGG GTGATGACAC AATGACGGGT 
GATGGGGGAG AGTACCTTAG ACCTGAGGAC CTAAAGGAGC TGGGTGATGA CTCCCTCCCC 
AGCAGTCAGT TCCTGGACGG GATGAATTAC CTGCGCTACA GCTTGGAGGG GGGCCGCTCC 
GACAGCCTTC GATCCTTCAG TTCCGACAGG TCTCACACCC TGAGCCATGC CTCGTACCTG 
AGGGACAGTG CCATGATCGA CGACGCAGTT GTGATCCCCA GTCACCAGGT GACAACTCTA 
GCCAAGGAGG CAGAAAGGAA TTCTTATCGT CTAAGCTGGG GCACTGAGAA CTTGGACAAC 
GTGGCTCTTT CTTCCAGCCC TATTCATTCA GGTTTCCTGG TTAGTTTTAT GGTGGATGCC 
CGAGGTGGTG CTATGCGAGG ATGCAGACAC AACGGGCTCC GCATCATTAT TCCGCCCCGG 
AAATGTACCG CCCCAACACG AGTCACCTGC CGACTGGTCA AACGCCATAG ACTGGCGACA 
ATGCCTCCAA TGGTGGAAGG AGAGGGCCTG GCCAGTCGCC TGATTGAAGT TGGACCTTCT 
GGTGCTCAGT TCCTTGGGCC TGTGATCGTG GAGATCCCTC ATTTCGCTGC CCTGCGAGGA 
AAAGAGAGGG AACTGGTGGT CCTGCGCAGT GAGAACGGGG ACAGCTGGAA AGAGCATTAC 
TGTGAATACA CGGAGGAGGA GCTGAATGAA ATCCTGAACG GCATGGATGA AGTGCTGGAT 
AGCCCGGAAG ACCTCGAAAA GAAGCGGATC TGCCGCATCA TCACCCGTGA CTTCCCACAG 
TACTTTGCGG TGGTGTCGCG CATCAAGCAG GACAGCAACC TGATTGGCCC GGAGGGAGGC 
GTGCTGAGCA GCACCGTGGT GCCGCAGGTG CAGGCCGTGT TCCCCGAGGG GGCGCTAACC 
AAGCGGATCC GTGTTGGCCT ACAGGCTCAA CCTATGCACA GTGAGTTGGT TAAGAAGATT 
CTGGGCAACA AAGCTACCTT CAGCCCTATA GTTACTTTGG AACCTAGAAG AAGAAAATTT 
CACAAGCCAA TTACCATGAC CATTCCTGTC CCCAAAGCTT CAAGTGATGT CATGTTGAAT 
GGTTTTGGAG GAGATGCACC AACCTTAAGA TTACTATGTA GCATCACAGG TGGAACCACC 
CCTGCTCAGT GGGAAGATAT TACAGGAACT ACGCCATTAA CATTTGTCAA TGAATGTGTT 
TCCTTTACAA CCAATGTGTC TGCCAGGTTC TGGCTGATCG ATTGTCGACA AATTCAGGAA 
TCTGTTACCT TTGCATCACA AGTGTATAGA GAAATTATCT GTGTACCATA TATGGCCAAA 
TTTGTAGTGT TTGCCAAATC ACATGACCCC ATTGAAGCCA GGTTGAGGTG TTTCTGCATG 
ACTGATGATA AAGTGGATAA AACCCTGGAA CAACAAGAAA ACTTTGCTGA GGTGGCCAGA 
AGCAGGGATG TGGAGGTCTT AGAAGGAAAA CCCATCTATG TTGATTGTTT TGGCAACCTG 
GTGCCATTAA CCAAGAGTGG CCAACATCAT ATATTCAGTT TTTTTGCCTT CAAAGAAAAC 
AGACTTCCTC TCTTTGTCAA GGTACGTGAT ACGACTCAGG AACCTTGCGG ACGACTATCA 
TTTATGAAAG AGCCAAAATC TACAAGAGGC CTGGTGCACC AAGCTATTTG CAACTTAAAC 
ATCACCCTGC CAATTTATAC GAAGGAATCA GAGTCAGATC AAGAACAGGA GGAAGAGATC 
GATATGACAT CAGAAAAAAA TCAACAGGAT GAGCAGGAAC GGATTGAGGA AAGGCTGGCA 
TATATTGCTG ATCACCTTGG CTTCAGCTGG ACAGAGTTGG CAAGAGAACT GGACTTCACT 
GAAGAGCAAA TTCATCAAAT TCGAATTGAG AACCCTAACT CCCTGCAGGA TCAGAGTCAT 
GCGCTGTTGA AGTACTGGCT AGAAAGGGAC GGGAGACATG CTACAGATAC CAGTCTCGTT 
GAATGTCTCA CCAAGATCAA CCGAATGGAT ATCGTGCATC TCATGGAGAC CAGCACAGAA 
GCTCTCCAGG AGCGCATCGG TCATAGCTAT GCAGAGATTG AGCAGACCAT TACACTGGAT 
CATAGTGAAG GGTTCTCAGT ACTCCAAGAG GAGCTTTGCA CTGCCCAGCA CAAGCAGAAA 
GAGGAGCATG CTGCTTCTAA AGAGGACGAG GCCTCTGACC ACCCTCCCAT CGTCTCAGAG 
GAAGACATTT CTGTTGGTTA CTCCACTCTT CAGGATGGCA TCCCCAAAAC TGAGGGTGAT 
GGCTCTGCAA CAGAGCTCCT TCCCCAGACT CAAAAGGAGC AAGTTCAACA GGATTTCTCA 
GGGAAAATGC AAGACCTGCC TGAGGAGTCG GCTCTTGAAC AGCAAGAATA CTTCGTGACA 
ACCCCAGGGA CAGAAATGTC AGAGACTCAG AAGGCCACGG GAACATCGGG CTCCCCCACC 
AAGACACCTG AGGAAATTGT CACCCCTTCC GAGGAGGAGA GGCTGTATCT CCAAACCCCA 
ACATCGAGTG AGCGGGGAGG CTCTCCAGTT GTGCAGGAAC CTGAGGAGCC CCCCGAACGC 
AGGGAGGAGA GTTCACCTCG GAAAACCAGC CTCGTGATCG TGGAATCGGC TGAGGACCAG 
CCTCAGGCCT TCGAGAGGCT TGATGAAGAG GCTGCTTTTC AAAAGGGAGA TGATATGCCT 
GACATACCCC CAGAAACAGT CACAGAAGAA GAATACATCG ATGAGCATGG ACACACCGTG 
GTAAAGAAGG TTACTAGGAA AATCATTAGG CGGTTCATCT CCTCTGACGG CACAGAGAAA 
GAGGAGATTA CGATGCAGGG AGCACCACAG GATTCTATCA GCGTTGATGA AGGGGATGGT 
TATTCCAAAG TCATAAAGCG CATTGTATTG AAGAGTGACA CCGAGCAGTC AGAGGTAACT 
TTGTCTGAGC CCAGCATTCT GTCCAGTACC TCACAATTTC AGGCTGAACC AGTGGAAGGC 
CGTAGAGTCA GCAAAGTTGT TAAGACAACT GTGGTCCATG GTGAGAGGAT GGAGAAACAT 
CTGGGGGATT CTAGTTTAGC CACTGATCTT CCTTCAGCCA AAGATGACTT TGAAGAGGCT 
TTGAGTTACA CAGGTAGCCA CATGAAAGTC TACTCCCCCA GTTTAGTAGA GAATGAAGTC 
CTGAAGGAGG ATGGATCAAT AATTAGAAGG ACGACAATGA GTAAAGCTAG AACCCAGAAG 
AGGGCTGTGG TGAAGGACCA GCAGGGAAAA CGCATTTACT TGGAGCACCT GGAGGATGTC 
CCAGAAGCGC TAGACCAGGA CGACCTCCAG CACGACCTCC AGCAACTCCT TCGGCATTTC 
TGCAAGGAGG ACTCGAAGCA AGAGGCCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK2 ( XM_024993493.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849261.1 Bos taurus ankyrin 2 (ANK2), transcript variant X9, mRNA.

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CloneID	OBa235485
Clone ID Related Accession (Same CDS sequence)	XM_024993493.1
Accession Version	XM_024993493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin-2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
AGTGGATTCA CCCCTTTGCA CATCGCTGCC CACTATGGAA ACGTCAACGT GGCAACTCTC 
CTTCTAAACC GGGGAGCTGC TGTGGACTTC ACAGCCAGGA ATGGAATCAC TCCTCTGCAT 
GTGGCCTCCA AAAGGGGAAA CACAAACATG GTGAAGCTCT TACTGGATCG TGGTGGTCAG 
ATTGATGCCA AAACTAGGGA TGGGTTGACA CCCCTTCATT GTGCTGCAAG AAGCGGTCAT 
GACCAAGTGG TGGAGCTTCT GCTGGAGCGG GGCGCTCCCT TGCTGGCAAG GACGAAGAAC 
GGGCTGTCTC CACTTCACAT GGCTGCACAG GGGGACCACG TGGAATGTGT GAAGCACCTG 
CTCCAGCACA AGGCGCCTGT TGATGACGTC ACTTTGGACT ACTTGACGGC CCTCCACGTT 
GCCGCACACT GCGGCCACTA CCGAGTAACC AAACTTCTTC TGGACAAGAG AGCCAACCCG 
AACGCCAGAG CCCTGAATGG TTTTACTCCA CTGCACATTG CCTGCAAGAA AAACCGCATC 
AAAGTCATGG AACTGCTGGT GAAATATGGG GCTTCAATCC AAGCTATAAC AGAGTCTGGC 
CTCACACCAA TACATGTGGC TGCCTTCATG GGCCACTTGA ACATTGTCCT CCTTCTGCTG 
CAGAACGGAG CCTCTCCAGA TGTCACTAAC ATTCGTGGGG AGACGGCCCT GCACATGGCG 
GCCCGTGCAG GGCAGGTGGA AGTGGTCCGA TGCCTCCTGA GGAACGGTGC CCTCGTCGAT 
GCCAGAGCCA GGGAGGAACA GACGCCTTTG CATATTGCCT CCCGCCTGGG TAAGACAGAA 
ATTGTTCAGC TGCTTCTGCA ACATATGGCT CATCCAGATG CGGCCACTAC AAATGGGTAC 
ACGCCGTTGC ATATCTCTGC AAGGGAAGGA CAGGTGGACG TGGCCTCGGT CCTCCTGGAA 
GCGGGGGCAG CCCACTCCTT GGCTACCAAG AAGGGTTTTA CCCCCTTGCA CGTAGCTGCC 
AAGTATGGGA GCCTGGATGT GGCAAAACTT CTCTTGCAAC GCCGTGCTGC CGCAGATTCT 
GCGGGGAAGA ATGGCTACAC TCCTTTACAT ATTGCTGCCA AGAAGAATCA AATGCAGATA 
GCTTCCACAC TCCTGAGCTA CGGAGCAGAG ACGAACATCG TGACAAAGCA AGGAGTCACT 
CCACTCCATC TGGCCTCGCA GGAGGGACAC ACGGATATGG TCACCTTGCT TCTGGATAAG 
GGAGCCAATA TCCACATGTC AACCAAGAGT GGACTCACAT CTTTACACCT TGCAGCCCAG 
GAAGATAAAG TGAATGTCGC TGATATTCTC ACCAAGCATG GGGCTGACCA GGATGCGCAC 
ACAAAGCTTG GTTACACTCC TTTAATTGTG GCCTGTCACT ATGGAAACGT GAAAATGGTC 
AACTTCCTTC TGAAGCAGGG AGCAAATGTC AATGCAAAAA CCAAGAATGG CTACACGCCT 
TTGCACCAAG CCGCCCAACA GGGCCACACG CACATCATCA ATGTTTTGCT GCAGCACGGG 
GCCAAGCCCA ACGCTACCAC CGCGAATGGC AACACTGCCT TGGCGATTGC TAAGCGTCTG 
GGCTACATCT CGGTGGTGGA CACCCTGAAG GTTGTAACGG AGGAGGTCAC CACCACCACC 
ACGACTATCA CTGAAAAACA CAAGCTAAAT GTTCCTGAGA CCATGACTGA GGTTCTTGAT 
GTTTCTGATG AAGAGGGTGA TGACACAATG ACGGGTGATG GGGGAGAGTA CCTTAGACCT 
GAGGACCTAA AGGAGCTGGG TGATGACTCC CTCCCCAGCA GTCAGTTCCT GGACGGGATG 
AATTACCTGC GCTACAGCTT GGAGGGGGGC CGCTCCGACA GCCTTCGATC CTTCAGTTCC 
GACAGGTCTC ACACCCTGAG CCATGCCTCG TACCTGAGGG ACAGTGCCAT GATCGACGAC 
GCAGTTGTGA TCCCCAGTCA CCAGGTGACA ACTCTAGCCA AGGAGGCAGA AAGGAATTCT 
TATCGTCTAA GCTGGGGCAC TGAGAACTTG GACAACGTGG CTCTTTCTTC CAGCCCTATT 
CATTCAGGTT TCCTGGTTAG TTTTATGGTG GATGCCCGAG GTGGTGCTAT GCGAGGATGC 
AGACACAACG GGCTCCGCAT CATTATTCCG CCCCGGAAAT GTACCGCCCC AACACGAGTC 
ACCTGCCGAC TGGTCAAACG CCATAGACTG GCGACAATGC CTCCAATGGT GGAAGGAGAG 
GGCCTGGCCA GTCGCCTGAT TGAAGTTGGA CCTTCTGGTG CTCAGTTCCT TGGGCCTGTG 
ATCGTGGAGA TCCCTCATTT CGCTGCCCTG CGAGGAAAAG AGAGGGAACT GGTGGTCCTG 
CGCAGTGAGA ACGGGGACAG CTGGAAAGAG CATTACTGTG AATACACGGA GGAGGAGCTG 
AATGAAATCC TGAACGGCAT GGATGAAGTG CTGGATAGCC CGGAAGACCT CGAAAAGAAG 
CGGATCTGCC GCATCATCAC CCGTGACTTC CCACAGTACT TTGCGGTGGT GTCGCGCATC 
AAGCAGGACA GCAACCTGAT TGGCCCGGAG GGAGGCGTGC TGAGCAGCAC CGTGGTGCCG 
CAGGTGCAGG CCGTGTTCCC CGAGGGGGCG CTAACCAAGC GGATCCGTGT TGGCCTACAG 
GCTCAACCTA TGCACAGTGA GTTGGTTAAG AAGATTCTGG GCAACAAAGC TACCTTCAGC 
CCTATAGTTA CTTTGGAACC TAGAAGAAGA AAATTTCACA AGCCAATTAC CATGACCATT 
CCTGTCCCCA AAGCTTCAAG TGATGTCATG TTGAATGGTT TTGGAGGAGA TGCACCAACC 
TTAAGATTAC TATGTAGCAT CACAGGTGGA ACCACCCCTG CTCAGTGGGA AGATATTACA 
GGAACTACGC CATTAACATT TGTCAATGAA TGTGTTTCCT TTACAACCAA TGTGTCTGCC 
AGGTTCTGGC TGATCGATTG TCGACAAATT CAGGAATCTG TTACCTTTGC ATCACAAGTG 
TATAGAGAAA TTATCTGTGT ACCATATATG GCCAAATTTG TAGTGTTTGC CAAATCACAT 
GACCCCATTG AAGCCAGGTT GAGGTGTTTC TGCATGACTG ATGATAAAGT GGATAAAACC 
CTGGAACAAC AAGAAAACTT TGCTGAGGTG GCCAGAAGCA GGGATGTGGA GGTCTTAGAA 
GGAAAACCCA TCTATGTTGA TTGTTTTGGC AACCTGGTGC CATTAACCAA GAGTGGCCAA 
CATCATATAT TCAGTTTTTT TGCCTTCAAA GAAAACAGAC TTCCTCTCTT TGTCAAGGTA 
CGTGATACGA CTCAGGAACC TTGCGGACGA CTATCATTTA TGAAAGAGCC AAAATCTACA 
AGAGGCCTGG TGCACCAAGC TATTTGCAAC TTAAACATCA CCCTGCCAAT TTATACGAAG 
GAATCAGAGT CAGATCAAGA ACAGGAGGAA GAGATCGATA TGACATCAGA AAAAAATCAA 
CAGGATGAGC AGGAACGGAT TGAGGAAAGG CTGGCATATA TTGCTGATCA CCTTGGCTTC 
AGCTGGACAG AGTTGGCAAG AGAACTGGAC TTCACTGAAG AGCAAATTCA TCAAATTCGA 
ATTGAGAACC CTAACTCCCT GCAGGATCAG AGTCATGCGC TGTTGAAGTA CTGGCTAGAA 
AGGGACGGGA GACATGCTAC AGATACCAGT CTCGTTGAAT GTCTCACCAA GATCAACCGA 
ATGGATATCG TGCATCTCAT GGAGACCAGC ACAGAAGCTC TCCAGGAGCG CATCGGTCAT 
AGCTATGCAG AGATTGAGCA GACCATTACA CTGGATCATA GTGAAGGGTT CTCAGTACTC 
CAAGAGGAGC TTTGCACTGC CCAGCACAAG CAGAAAGAGG AGCATGCTGC TTCTAAAGAG 
GACGAGGCCT CTGACCACCC TCCCATCGTC TCAGAGGAAG ACATTTCTGT TGGTTACTCC 
ACTCTTCAGG ATGGCATCCC CAAAACTGAG GGTGATGGCT CTGCAACAGA GCTCCTTCCC 
CAGACTCAAA AGGAGCAAGT TCAACAGGAT TTCTCAGGGA AAATGCAAGA CCTGCCTGAG 
GAGTCGGCTC TTGAACAGCA AGAATACTTC GTGACAACCC CAGGGACAGA AATGTCAGAG 
ACTCAGAAGG CCACGGGAAC ATCGGGCTCC CCCACCAAGA CACCTGAGGA AATTGTCACC 
CCTTCCGAGG AGGAGAGGCT GTATCTCCAA ACCCCAACAT CGAGTGAGCG GGGAGGCTCT 
CCAGTTGTGC AGGAACCTGA GGAGCCCCCC GAACGCAGGG AGGAGAGTTC ACCTCGGAAA 
ACCAGCCTCG TGATCGTGGA ATCGGCTGAG GACCAGCCTC AGGCCTTCGA GAGGCTTGAT 
GAAGAGGCTG CTTTTCAAAA GGGAGATGAT ATGCCTGACA TACCCCCAGA AACAGTCACA 
GAAGAAGAAT ACATCGATGA GCATGGACAC ACCGTGGTAA AGAAGGTTAC TAGGAAAATC 
ATTAGGCGGT TCATCTCCTC TGACGGCACA GAGAAAGAGG AGATTACGAT GCAGGGAGCA 
CCACAGGATT CTATCAGCGT TGATGAAGGG GATGGTTATT CCAAAGTCAT AAAGCGCATT 
GTATTGAAGA GTGACACCGA GCAGTCAGAG GTAACTTTGT CTGAGCCCAG CATTCTGTCC 
AGTACCTCAC AATTTCAGGC TGAACCAGTG GAAGGCCGTA GAGTCAGCAA AGTTGTTAAG 
ACAACTGTGG TCCATGGTGA GAGGATGGAG AAACATCTGG GGGATTCTAG TTTAGCCACT 
GATCTTCCTT CAGCCAAAGA TGACTTTGAA GAGGCTTTGA GTTACACAGG TAGCCACATG 
AAAGTCTACT CCCCCAGTTT AGTAGAGAAT GAAGTCCTGA AGGAGGATGG ATCAATAATT 
AGAAGGACGA CAATGAGTAA AGCTAGAACC CAGAAGAGGG CTGTGGTGAA GGACCAGCAG 
GGAAAACGCA TTTACTTGGA GCACCTGGAG GATGTCCCAG AAGCGCTAGA CCAGGACGAC 
CTCCAGCACG ACCTCCAGCA ACTCCTTCGG CATTTCTGCA AGGAGGACTC GAAGCAAGAG 
GCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849261.1
CDS	238..6126
Translation	MGNAALCPACHADKSVRAQGHLQELDRTPDYYGCNGEDTKELGPRSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSAVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQIASTLLSYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMTEVLDVSDEEGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSHTLSHASYLRDSAMIDDAVVIPSHQVTTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGFLVSFMVDARGGAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHYCEYTEEELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEIDMTSEKNQQDEQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGRHATDTSLVECLTKINRMDIVHLMETSTEALQERIGHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEHAASKEDEASDHPPIVSEEDISVGYSTLQDGIPKTEGDGSATELLPQTQKEQVQQDFSGKMQDLPEESALEQQEYFVTTPGTEMSETQKATGTSGSPTKTPEEIVTPSEEERLYLQTPTSSERGGSPVVQEPEEPPERREESSPRKTSLVIVESAEDQPQAFERLDEEAAFQKGDDMPDIPPETVTEEEYIDEHGHTVVKKVTRKIIRRFISSDGTEKEEITMQGAPQDSISVDEGDGYSKVIKRIVLKSDTEQSEVTLSEPSILSSTSQFQAEPVEGRRVSKVVKTTVVHGERMEKHLGDSSLATDLPSAKDDFEEALSYTGSHMKVYSPSLVENEVLKEDGSIIRRTTMSKARTQKRAVVKDQQGKRIYLEHLEDVPEALDQDDLQHDLQQLLRHFCKEDSKQEAQ

Target ORF information:

RefSeq Version	XM_024993493.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin 2 (ANK2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993493.1

ORF Insert Sequence:

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
AGTGGATTCA CCCCTTTGCA CATCGCTGCC CACTATGGAA ACGTCAACGT GGCAACTCTC 
CTTCTAAACC GGGGAGCTGC TGTGGACTTC ACAGCCAGGA ATGGAATCAC TCCTCTGCAT 
GTGGCCTCCA AAAGGGGAAA CACAAACATG GTGAAGCTCT TACTGGATCG TGGTGGTCAG 
ATTGATGCCA AAACTAGGGA TGGGTTGACA CCCCTTCATT GTGCTGCAAG AAGCGGTCAT 
GACCAAGTGG TGGAGCTTCT GCTGGAGCGG GGCGCTCCCT TGCTGGCAAG GACGAAGAAC 
GGGCTGTCTC CACTTCACAT GGCTGCACAG GGGGACCACG TGGAATGTGT GAAGCACCTG 
CTCCAGCACA AGGCGCCTGT TGATGACGTC ACTTTGGACT ACTTGACGGC CCTCCACGTT 
GCCGCACACT GCGGCCACTA CCGAGTAACC AAACTTCTTC TGGACAAGAG AGCCAACCCG 
AACGCCAGAG CCCTGAATGG TTTTACTCCA CTGCACATTG CCTGCAAGAA AAACCGCATC 
AAAGTCATGG AACTGCTGGT GAAATATGGG GCTTCAATCC AAGCTATAAC AGAGTCTGGC 
CTCACACCAA TACATGTGGC TGCCTTCATG GGCCACTTGA ACATTGTCCT CCTTCTGCTG 
CAGAACGGAG CCTCTCCAGA TGTCACTAAC ATTCGTGGGG AGACGGCCCT GCACATGGCG 
GCCCGTGCAG GGCAGGTGGA AGTGGTCCGA TGCCTCCTGA GGAACGGTGC CCTCGTCGAT 
GCCAGAGCCA GGGAGGAACA GACGCCTTTG CATATTGCCT CCCGCCTGGG TAAGACAGAA 
ATTGTTCAGC TGCTTCTGCA ACATATGGCT CATCCAGATG CGGCCACTAC AAATGGGTAC 
ACGCCGTTGC ATATCTCTGC AAGGGAAGGA CAGGTGGACG TGGCCTCGGT CCTCCTGGAA 
GCGGGGGCAG CCCACTCCTT GGCTACCAAG AAGGGTTTTA CCCCCTTGCA CGTAGCTGCC 
AAGTATGGGA GCCTGGATGT GGCAAAACTT CTCTTGCAAC GCCGTGCTGC CGCAGATTCT 
GCGGGGAAGA ATGGCTACAC TCCTTTACAT ATTGCTGCCA AGAAGAATCA AATGCAGATA 
GCTTCCACAC TCCTGAGCTA CGGAGCAGAG ACGAACATCG TGACAAAGCA AGGAGTCACT 
CCACTCCATC TGGCCTCGCA GGAGGGACAC ACGGATATGG TCACCTTGCT TCTGGATAAG 
GGAGCCAATA TCCACATGTC AACCAAGAGT GGACTCACAT CTTTACACCT TGCAGCCCAG 
GAAGATAAAG TGAATGTCGC TGATATTCTC ACCAAGCATG GGGCTGACCA GGATGCGCAC 
ACAAAGCTTG GTTACACTCC TTTAATTGTG GCCTGTCACT ATGGAAACGT GAAAATGGTC 
AACTTCCTTC TGAAGCAGGG AGCAAATGTC AATGCAAAAA CCAAGAATGG CTACACGCCT 
TTGCACCAAG CCGCCCAACA GGGCCACACG CACATCATCA ATGTTTTGCT GCAGCACGGG 
GCCAAGCCCA ACGCTACCAC CGCGAATGGC AACACTGCCT TGGCGATTGC TAAGCGTCTG 
GGCTACATCT CGGTGGTGGA CACCCTGAAG GTTGTAACGG AGGAGGTCAC CACCACCACC 
ACGACTATCA CTGAAAAACA CAAGCTAAAT GTTCCTGAGA CCATGACTGA GGTTCTTGAT 
GTTTCTGATG AAGAGGGTGA TGACACAATG ACGGGTGATG GGGGAGAGTA CCTTAGACCT 
GAGGACCTAA AGGAGCTGGG TGATGACTCC CTCCCCAGCA GTCAGTTCCT GGACGGGATG 
AATTACCTGC GCTACAGCTT GGAGGGGGGC CGCTCCGACA GCCTTCGATC CTTCAGTTCC 
GACAGGTCTC ACACCCTGAG CCATGCCTCG TACCTGAGGG ACAGTGCCAT GATCGACGAC 
GCAGTTGTGA TCCCCAGTCA CCAGGTGACA ACTCTAGCCA AGGAGGCAGA AAGGAATTCT 
TATCGTCTAA GCTGGGGCAC TGAGAACTTG GACAACGTGG CTCTTTCTTC CAGCCCTATT 
CATTCAGGTT TCCTGGTTAG TTTTATGGTG GATGCCCGAG GTGGTGCTAT GCGAGGATGC 
AGACACAACG GGCTCCGCAT CATTATTCCG CCCCGGAAAT GTACCGCCCC AACACGAGTC 
ACCTGCCGAC TGGTCAAACG CCATAGACTG GCGACAATGC CTCCAATGGT GGAAGGAGAG 
GGCCTGGCCA GTCGCCTGAT TGAAGTTGGA CCTTCTGGTG CTCAGTTCCT TGGGCCTGTG 
ATCGTGGAGA TCCCTCATTT CGCTGCCCTG CGAGGAAAAG AGAGGGAACT GGTGGTCCTG 
CGCAGTGAGA ACGGGGACAG CTGGAAAGAG CATTACTGTG AATACACGGA GGAGGAGCTG 
AATGAAATCC TGAACGGCAT GGATGAAGTG CTGGATAGCC CGGAAGACCT CGAAAAGAAG 
CGGATCTGCC GCATCATCAC CCGTGACTTC CCACAGTACT TTGCGGTGGT GTCGCGCATC 
AAGCAGGACA GCAACCTGAT TGGCCCGGAG GGAGGCGTGC TGAGCAGCAC CGTGGTGCCG 
CAGGTGCAGG CCGTGTTCCC CGAGGGGGCG CTAACCAAGC GGATCCGTGT TGGCCTACAG 
GCTCAACCTA TGCACAGTGA GTTGGTTAAG AAGATTCTGG GCAACAAAGC TACCTTCAGC 
CCTATAGTTA CTTTGGAACC TAGAAGAAGA AAATTTCACA AGCCAATTAC CATGACCATT 
CCTGTCCCCA AAGCTTCAAG TGATGTCATG TTGAATGGTT TTGGAGGAGA TGCACCAACC 
TTAAGATTAC TATGTAGCAT CACAGGTGGA ACCACCCCTG CTCAGTGGGA AGATATTACA 
GGAACTACGC CATTAACATT TGTCAATGAA TGTGTTTCCT TTACAACCAA TGTGTCTGCC 
AGGTTCTGGC TGATCGATTG TCGACAAATT CAGGAATCTG TTACCTTTGC ATCACAAGTG 
TATAGAGAAA TTATCTGTGT ACCATATATG GCCAAATTTG TAGTGTTTGC CAAATCACAT 
GACCCCATTG AAGCCAGGTT GAGGTGTTTC TGCATGACTG ATGATAAAGT GGATAAAACC 
CTGGAACAAC AAGAAAACTT TGCTGAGGTG GCCAGAAGCA GGGATGTGGA GGTCTTAGAA 
GGAAAACCCA TCTATGTTGA TTGTTTTGGC AACCTGGTGC CATTAACCAA GAGTGGCCAA 
CATCATATAT TCAGTTTTTT TGCCTTCAAA GAAAACAGAC TTCCTCTCTT TGTCAAGGTA 
CGTGATACGA CTCAGGAACC TTGCGGACGA CTATCATTTA TGAAAGAGCC AAAATCTACA 
AGAGGCCTGG TGCACCAAGC TATTTGCAAC TTAAACATCA CCCTGCCAAT TTATACGAAG 
GAATCAGAGT CAGATCAAGA ACAGGAGGAA GAGATCGATA TGACATCAGA AAAAAATCAA 
CAGGATGAGC AGGAACGGAT TGAGGAAAGG CTGGCATATA TTGCTGATCA CCTTGGCTTC 
AGCTGGACAG AGTTGGCAAG AGAACTGGAC TTCACTGAAG AGCAAATTCA TCAAATTCGA 
ATTGAGAACC CTAACTCCCT GCAGGATCAG AGTCATGCGC TGTTGAAGTA CTGGCTAGAA 
AGGGACGGGA GACATGCTAC AGATACCAGT CTCGTTGAAT GTCTCACCAA GATCAACCGA 
ATGGATATCG TGCATCTCAT GGAGACCAGC ACAGAAGCTC TCCAGGAGCG CATCGGTCAT 
AGCTATGCAG AGATTGAGCA GACCATTACA CTGGATCATA GTGAAGGGTT CTCAGTACTC 
CAAGAGGAGC TTTGCACTGC CCAGCACAAG CAGAAAGAGG AGCATGCTGC TTCTAAAGAG 
GACGAGGCCT CTGACCACCC TCCCATCGTC TCAGAGGAAG ACATTTCTGT TGGTTACTCC 
ACTCTTCAGG ATGGCATCCC CAAAACTGAG GGTGATGGCT CTGCAACAGA GCTCCTTCCC 
CAGACTCAAA AGGAGCAAGT TCAACAGGAT TTCTCAGGGA AAATGCAAGA CCTGCCTGAG 
GAGTCGGCTC TTGAACAGCA AGAATACTTC GTGACAACCC CAGGGACAGA AATGTCAGAG 
ACTCAGAAGG CCACGGGAAC ATCGGGCTCC CCCACCAAGA CACCTGAGGA AATTGTCACC 
CCTTCCGAGG AGGAGAGGCT GTATCTCCAA ACCCCAACAT CGAGTGAGCG GGGAGGCTCT 
CCAGTTGTGC AGGAACCTGA GGAGCCCCCC GAACGCAGGG AGGAGAGTTC ACCTCGGAAA 
ACCAGCCTCG TGATCGTGGA ATCGGCTGAG GACCAGCCTC AGGCCTTCGA GAGGCTTGAT 
GAAGAGGCTG CTTTTCAAAA GGGAGATGAT ATGCCTGACA TACCCCCAGA AACAGTCACA 
GAAGAAGAAT ACATCGATGA GCATGGACAC ACCGTGGTAA AGAAGGTTAC TAGGAAAATC 
ATTAGGCGGT TCATCTCCTC TGACGGCACA GAGAAAGAGG AGATTACGAT GCAGGGAGCA 
CCACAGGATT CTATCAGCGT TGATGAAGGG GATGGTTATT CCAAAGTCAT AAAGCGCATT 
GTATTGAAGA GTGACACCGA GCAGTCAGAG GTAACTTTGT CTGAGCCCAG CATTCTGTCC 
AGTACCTCAC AATTTCAGGC TGAACCAGTG GAAGGCCGTA GAGTCAGCAA AGTTGTTAAG 
ACAACTGTGG TCCATGGTGA GAGGATGGAG AAACATCTGG GGGATTCTAG TTTAGCCACT 
GATCTTCCTT CAGCCAAAGA TGACTTTGAA GAGGCTTTGA GTTACACAGG TAGCCACATG 
AAAGTCTACT CCCCCAGTTT AGTAGAGAAT GAAGTCCTGA AGGAGGATGG ATCAATAATT 
AGAAGGACGA CAATGAGTAA AGCTAGAACC CAGAAGAGGG CTGTGGTGAA GGACCAGCAG 
GGAAAACGCA TTTACTTGGA GCACCTGGAG GATGTCCCAG AAGCGCTAGA CCAGGACGAC 
CTCCAGCACG ACCTCCAGCA ACTCCTTCGG CATTTCTGCA AGGAGGACTC GAAGCAAGAG 
GCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK2 ( XM_024993494.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849262.1 Bos taurus ankyrin 2 (ANK2), transcript variant X10, mRNA.

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CloneID	OBa235486
Clone ID Related Accession (Same CDS sequence)	XM_024993494.1
Accession Version	XM_024993494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin-2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
AGTGGATTCA CCCCTTTGCA CATCGCTGCC CACTATGGAA ACGTCAACGT GGCAACTCTC 
CTTCTAAACC GGGGAGCTGC TGTGGACTTC ACAGCCAGGA ATGGAATCAC TCCTCTGCAT 
GTGGCCTCCA AAAGGGGAAA CACAAACATG GTGAAGCTCT TACTGGATCG TGGTGGTCAG 
ATTGATGCCA AAACTAGGGA TGGGTTGACA CCCCTTCATT GTGCTGCAAG AAGCGGTCAT 
GACCAAGTGG TGGAGCTTCT GCTGGAGCGG GGCGCTCCCT TGCTGGCAAG GACGAAGAAC 
GGGCTGTCTC CACTTCACAT GGCTGCACAG GGGGACCACG TGGAATGTGT GAAGCACCTG 
CTCCAGCACA AGGCGCCTGT TGATGACGTC ACTTTGGACT ACTTGACGGC CCTCCACGTT 
GCCGCACACT GCGGCCACTA CCGAGTAACC AAACTTCTTC TGGACAAGAG AGCCAACCCG 
AACGCCAGAG CCCTGCGTGG GGAGACGGCC CTGCACATGG CGGCCCGTGC AGGGCAGGTG 
GAAGTGGTCC GATGCCTCCT GAGGAACGGT GCCCTCGTCG ATGCCAGAGC CAGGGAGGAA 
CAGACGCCTT TGCATATTGC CTCCCGCCTG GGTAAGACAG AAATTGTTCA GCTGCTTCTG 
CAACATATGG CTCATCCAGA TGCGGCCACT ACAAATGGGT ACACGCCGTT GCATATCTCT 
GCAAGGGAAG GACAGGTGGA CGTGGCCTCG GTCCTCCTGG AAGCGGGGGC AGCCCACTCC 
TTGGCTACCA AGAAGGGTTT TACCCCCTTG CACGTAGCTG CCAAGTATGG GAGCCTGGAT 
GTGGCAAAAC TTCTCTTGCA ACGCCGTGCT GCCGCAGATT CTGCGGGGAA GAATGGCCTT 
ACCCCGCTCC ATGTTGCTGC TCACTATGAC AACCAGAAGG TGGCGCTGCT GTTACTGGAG 
AAGGGTGCTT CCCCTCACGC CATGGCCAAG AATGGCTACA CTCCTTTACA TATTGCTGCC 
AAGAAGAATC AAATGCAGAT AGCTTCCACA CTCCTGAGCT ACGGAGCAGA GACGAACATC 
GTGACAAAGC AAGGAGTCAC TCCACTCCAT CTGGCCTCGC AGGAGGGACA CACGGATATG 
GTCACCTTGC TTCTGGATAA GGGAGCCAAT ATCCACATGT CAACCAAGAG TGGACTCACA 
TCTTTACACC TTGCAGCCCA GGAAGATAAA GTGAATGTCG CTGATATTCT CACCAAGCAT 
GGGGCTGACC AGGATGCGCA CACAAAGCTT GGTTACACTC CTTTAATTGT GGCCTGTCAC 
TATGGAAACG TGAAAATGGT CAACTTCCTT CTGAAGCAGG GAGCAAATGT CAATGCAAAA 
ACCAAGAATG GCTACACGCC TTTGCACCAA GCCGCCCAAC AGGGCCACAC GCACATCATC 
AATGTTTTGC TGCAGCACGG GGCCAAGCCC AACGCTACCA CCGCGAATGG CAACACTGCC 
TTGGCGATTG CTAAGCGTCT GGGCTACATC TCGGTGGTGG ACACCCTGAA GGTTGTAACG 
GAGGAGGTCA CCACCACCAC CACGACTATC ACTGAAAAAC ACAAGCTAAA TGTTCCTGAG 
ACCATGACTG AGGTTCTTGA TGTTTCTGAT GAAGAGGGTG ATGACACAAT GACGGGTGAT 
GGGGGAGAGT ACCTTAGACC TGAGGACCTA AAGGAGCTGG GTGATGACTC CCTCCCCAGC 
AGTCAGTTCC TGGACGGGAT GAATTACCTG CGCTACAGCT TGGAGGGGGG CCGCTCCGAC 
AGCCTTCGAT CCTTCAGTTC CGACAGGTCT CACACCCTGA GCCATGCCTC GTACCTGAGG 
GACAGTGCCA TGATCGACGA CGCAGTTGTG ATCCCCAGTC ACCAGGTGAC AACTCTAGCC 
AAGGAGGCAG AAAGGAATTC TTATCGTCTA AGCTGGGGCA CTGAGAACTT GGACAACGTG 
GCTCTTTCTT CCAGCCCTAT TCATTCAGGT TTCCTGGTTA GTTTTATGGT GGATGCCCGA 
GGTGGTGCTA TGCGAGGATG CAGACACAAC GGGCTCCGCA TCATTATTCC GCCCCGGAAA 
TGTACCGCCC CAACACGAGT CACCTGCCGA CTGGTCAAAC GCCATAGACT GGCGACAATG 
CCTCCAATGG TGGAAGGAGA GGGCCTGGCC AGTCGCCTGA TTGAAGTTGG ACCTTCTGGT 
GCTCAGTTCC TTGGGCCTGT GATCGTGGAG ATCCCTCATT TCGCTGCCCT GCGAGGAAAA 
GAGAGGGAAC TGGTGGTCCT GCGCAGTGAG AACGGGGACA GCTGGAAAGA GCATTACTGT 
GAATACACGG AGGAGGAGCT GAATGAAATC CTGAACGGCA TGGATGAAGT GCTGGATAGC 
CCGGAAGACC TCGAAAAGAA GCGGATCTGC CGCATCATCA CCCGTGACTT CCCACAGTAC 
TTTGCGGTGG TGTCGCGCAT CAAGCAGGAC AGCAACCTGA TTGGCCCGGA GGGAGGCGTG 
CTGAGCAGCA CCGTGGTGCC GCAGGTGCAG GCCGTGTTCC CCGAGGGGGC GCTAACCAAG 
CGGATCCGTG TTGGCCTACA GGCTCAACCT ATGCACAGTG AGTTGGTTAA GAAGATTCTG 
GGCAACAAAG CTACCTTCAG CCCTATAGTT ACTTTGGAAC CTAGAAGAAG AAAATTTCAC 
AAGCCAATTA CCATGACCAT TCCTGTCCCC AAAGCTTCAA GTGATGTCAT GTTGAATGGT 
TTTGGAGGAG ATGCACCAAC CTTAAGATTA CTATGTAGCA TCACAGGTGG AACCACCCCT 
GCTCAGTGGG AAGATATTAC AGGAACTACG CCATTAACAT TTGTCAATGA ATGTGTTTCC 
TTTACAACCA ATGTGTCTGC CAGGTTCTGG CTGATCGATT GTCGACAAAT TCAGGAATCT 
GTTACCTTTG CATCACAAGT GTATAGAGAA ATTATCTGTG TACCATATAT GGCCAAATTT 
GTAGTGTTTG CCAAATCACA TGACCCCATT GAAGCCAGGT TGAGGTGTTT CTGCATGACT 
GATGATAAAG TGGATAAAAC CCTGGAACAA CAAGAAAACT TTGCTGAGGT GGCCAGAAGC 
AGGGATGTGG AGGTCTTAGA AGGAAAACCC ATCTATGTTG ATTGTTTTGG CAACCTGGTG 
CCATTAACCA AGAGTGGCCA ACATCATATA TTCAGTTTTT TTGCCTTCAA AGAAAACAGA 
CTTCCTCTCT TTGTCAAGGT ACGTGATACG ACTCAGGAAC CTTGCGGACG ACTATCATTT 
ATGAAAGAGC CAAAATCTAC AAGAGGCCTG GTGCACCAAG CTATTTGCAA CTTAAACATC 
ACCCTGCCAA TTTATACGAA GGAATCAGAG TCAGATCAAG AACAGGAGGA AGAGATCGAT 
ATGACATCAG AAAAAAATCA ACAGGATGAG CAGGAACGGA TTGAGGAAAG GCTGGCATAT 
ATTGCTGATC ACCTTGGCTT CAGCTGGACA GAGTTGGCAA GAGAACTGGA CTTCACTGAA 
GAGCAAATTC ATCAAATTCG AATTGAGAAC CCTAACTCCC TGCAGGATCA GAGTCATGCG 
CTGTTGAAGT ACTGGCTAGA AAGGGACGGG AGACATGCTA CAGATACCAG TCTCGTTGAA 
TGTCTCACCA AGATCAACCG AATGGATATC GTGCATCTCA TGGAGACCAG CACAGAAGCT 
CTCCAGGAGC GCATCGGTCA TAGCTATGCA GAGATTGAGC AGACCATTAC ACTGGATCAT 
AGTGAAGGGT TCTCAGTACT CCAAGAGGAG CTTTGCACTG CCCAGCACAA GCAGAAAGAG 
GAGCATGCTG CTTCTAAAGA GGACGAGGCC TCTGACCACC CTCCCATCGT CTCAGAGGAA 
GACATTTCTG TTGGTTACTC CACTCTTCAG GATGGCATCC CCAAAACTGA GGGTGATGGC 
TCTGCAACAG AGCTCCTTCC CCAGACTCAA AAGGAGCAAG TTCAACAGGA TTTCTCAGGG 
AAAATGCAAG ACCTGCCTGA GGAGTCGGCT CTTGAACAGC AAGAATACTT CGTGACAACC 
CCAGGGACAG AAATGTCAGA GACTCAGAAG GCCACGGGAA CATCGGGCTC CCCCACCAAG 
ACACCTGAGG AAATTGTCAC CCCTTCCGAG GAGGAGAGGC TGTATCTCCA AACCCCAACA 
TCGAGTGAGC GGGGAGGCTC TCCAGTTGTG CAGGAACCTG AGGAGCCCCC CGAACGCAGG 
GAGGAGAGTT CACCTCGGAA AACCAGCCTC GTGATCGTGG AATCGGCTGA GGACCAGCCT 
CAGGCCTTCG AGAGGCTTGA TGAAGAGGCT GCTTTTCAAA AGGGAGATGA TATGCCTGAC 
ATACCCCCAG AAACAGTCAC AGAAGAAGAA TACATCGATG AGCATGGACA CACCGTGGTA 
AAGAAGGTTA CTAGGAAAAT CATTAGGCGG TTCATCTCCT CTGACGGCAC AGAGAAAGAG 
GAGATTACGA TGCAGGGAGC ACCACAGGAT TCTATCAGCG TTGATGAAGG GGATGGTTAT 
TCCAAAGTCA TAAAGCGCAT TGTATTGAAG AGTGACACCG AGCAGTCAGA GGTAACTTTG 
TCTGAGCCCA GCATTCTGTC CAGTACCTCA CAATTTCAGG CTGAACCAGT GGAAGGCCGT 
AGAGTCAGCA AAGTTGTTAA GACAACTGTG GTCCATGGTG AGAGGATGGA GAAACATCTG 
GGGGATTCTA GTTTAGCCAC TGATCTTCCT TCAGCCAAAG ATGACTTTGA AGAGGCTTTG 
AGTTACACAG GTAGCCACAT GAAAGTCTAC TCCCCCAGTT TAGTAGAGAA TGAAGTCCTG 
AAGGAGGATG GATCAATAAT TAGAAGGACG ACAATGAGTA AAGCTAGAAC CCAGAAGAGG 
GCTGTGGTGA AGGACCAGCA GGGAAAACGC ATTTACTTGG AGCACCTGGA GGATGTCCCA 
GAAGCGCTAG ACCAGGACGA CCTCCAGCAC GACCTCCAGC AACTCCTTCG GCATTTCTGC 
AAGGAGGACT CGAAGCAAGA GGCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849262.1
CDS	238..6027
Translation	MGNAALCPACHADKSVRAQGHLQELDRTPDYYGCNGEDTKELGPRSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSAVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHAMAKNGYTPLHIAAKKNQMQIASTLLSYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMTEVLDVSDEEGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSHTLSHASYLRDSAMIDDAVVIPSHQVTTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGFLVSFMVDARGGAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHYCEYTEEELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEIDMTSEKNQQDEQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGRHATDTSLVECLTKINRMDIVHLMETSTEALQERIGHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEHAASKEDEASDHPPIVSEEDISVGYSTLQDGIPKTEGDGSATELLPQTQKEQVQQDFSGKMQDLPEESALEQQEYFVTTPGTEMSETQKATGTSGSPTKTPEEIVTPSEEERLYLQTPTSSERGGSPVVQEPEEPPERREESSPRKTSLVIVESAEDQPQAFERLDEEAAFQKGDDMPDIPPETVTEEEYIDEHGHTVVKKVTRKIIRRFISSDGTEKEEITMQGAPQDSISVDEGDGYSKVIKRIVLKSDTEQSEVTLSEPSILSSTSQFQAEPVEGRRVSKVVKTTVVHGERMEKHLGDSSLATDLPSAKDDFEEALSYTGSHMKVYSPSLVENEVLKEDGSIIRRTTMSKARTQKRAVVKDQQGKRIYLEHLEDVPEALDQDDLQHDLQQLLRHFCKEDSKQEAQ

Target ORF information:

RefSeq Version	XM_024993494.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin 2 (ANK2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993494.1

ORF Insert Sequence:

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
AGTGGATTCA CCCCTTTGCA CATCGCTGCC CACTATGGAA ACGTCAACGT GGCAACTCTC 
CTTCTAAACC GGGGAGCTGC TGTGGACTTC ACAGCCAGGA ATGGAATCAC TCCTCTGCAT 
GTGGCCTCCA AAAGGGGAAA CACAAACATG GTGAAGCTCT TACTGGATCG TGGTGGTCAG 
ATTGATGCCA AAACTAGGGA TGGGTTGACA CCCCTTCATT GTGCTGCAAG AAGCGGTCAT 
GACCAAGTGG TGGAGCTTCT GCTGGAGCGG GGCGCTCCCT TGCTGGCAAG GACGAAGAAC 
GGGCTGTCTC CACTTCACAT GGCTGCACAG GGGGACCACG TGGAATGTGT GAAGCACCTG 
CTCCAGCACA AGGCGCCTGT TGATGACGTC ACTTTGGACT ACTTGACGGC CCTCCACGTT 
GCCGCACACT GCGGCCACTA CCGAGTAACC AAACTTCTTC TGGACAAGAG AGCCAACCCG 
AACGCCAGAG CCCTGCGTGG GGAGACGGCC CTGCACATGG CGGCCCGTGC AGGGCAGGTG 
GAAGTGGTCC GATGCCTCCT GAGGAACGGT GCCCTCGTCG ATGCCAGAGC CAGGGAGGAA 
CAGACGCCTT TGCATATTGC CTCCCGCCTG GGTAAGACAG AAATTGTTCA GCTGCTTCTG 
CAACATATGG CTCATCCAGA TGCGGCCACT ACAAATGGGT ACACGCCGTT GCATATCTCT 
GCAAGGGAAG GACAGGTGGA CGTGGCCTCG GTCCTCCTGG AAGCGGGGGC AGCCCACTCC 
TTGGCTACCA AGAAGGGTTT TACCCCCTTG CACGTAGCTG CCAAGTATGG GAGCCTGGAT 
GTGGCAAAAC TTCTCTTGCA ACGCCGTGCT GCCGCAGATT CTGCGGGGAA GAATGGCCTT 
ACCCCGCTCC ATGTTGCTGC TCACTATGAC AACCAGAAGG TGGCGCTGCT GTTACTGGAG 
AAGGGTGCTT CCCCTCACGC CATGGCCAAG AATGGCTACA CTCCTTTACA TATTGCTGCC 
AAGAAGAATC AAATGCAGAT AGCTTCCACA CTCCTGAGCT ACGGAGCAGA GACGAACATC 
GTGACAAAGC AAGGAGTCAC TCCACTCCAT CTGGCCTCGC AGGAGGGACA CACGGATATG 
GTCACCTTGC TTCTGGATAA GGGAGCCAAT ATCCACATGT CAACCAAGAG TGGACTCACA 
TCTTTACACC TTGCAGCCCA GGAAGATAAA GTGAATGTCG CTGATATTCT CACCAAGCAT 
GGGGCTGACC AGGATGCGCA CACAAAGCTT GGTTACACTC CTTTAATTGT GGCCTGTCAC 
TATGGAAACG TGAAAATGGT CAACTTCCTT CTGAAGCAGG GAGCAAATGT CAATGCAAAA 
ACCAAGAATG GCTACACGCC TTTGCACCAA GCCGCCCAAC AGGGCCACAC GCACATCATC 
AATGTTTTGC TGCAGCACGG GGCCAAGCCC AACGCTACCA CCGCGAATGG CAACACTGCC 
TTGGCGATTG CTAAGCGTCT GGGCTACATC TCGGTGGTGG ACACCCTGAA GGTTGTAACG 
GAGGAGGTCA CCACCACCAC CACGACTATC ACTGAAAAAC ACAAGCTAAA TGTTCCTGAG 
ACCATGACTG AGGTTCTTGA TGTTTCTGAT GAAGAGGGTG ATGACACAAT GACGGGTGAT 
GGGGGAGAGT ACCTTAGACC TGAGGACCTA AAGGAGCTGG GTGATGACTC CCTCCCCAGC 
AGTCAGTTCC TGGACGGGAT GAATTACCTG CGCTACAGCT TGGAGGGGGG CCGCTCCGAC 
AGCCTTCGAT CCTTCAGTTC CGACAGGTCT CACACCCTGA GCCATGCCTC GTACCTGAGG 
GACAGTGCCA TGATCGACGA CGCAGTTGTG ATCCCCAGTC ACCAGGTGAC AACTCTAGCC 
AAGGAGGCAG AAAGGAATTC TTATCGTCTA AGCTGGGGCA CTGAGAACTT GGACAACGTG 
GCTCTTTCTT CCAGCCCTAT TCATTCAGGT TTCCTGGTTA GTTTTATGGT GGATGCCCGA 
GGTGGTGCTA TGCGAGGATG CAGACACAAC GGGCTCCGCA TCATTATTCC GCCCCGGAAA 
TGTACCGCCC CAACACGAGT CACCTGCCGA CTGGTCAAAC GCCATAGACT GGCGACAATG 
CCTCCAATGG TGGAAGGAGA GGGCCTGGCC AGTCGCCTGA TTGAAGTTGG ACCTTCTGGT 
GCTCAGTTCC TTGGGCCTGT GATCGTGGAG ATCCCTCATT TCGCTGCCCT GCGAGGAAAA 
GAGAGGGAAC TGGTGGTCCT GCGCAGTGAG AACGGGGACA GCTGGAAAGA GCATTACTGT 
GAATACACGG AGGAGGAGCT GAATGAAATC CTGAACGGCA TGGATGAAGT GCTGGATAGC 
CCGGAAGACC TCGAAAAGAA GCGGATCTGC CGCATCATCA CCCGTGACTT CCCACAGTAC 
TTTGCGGTGG TGTCGCGCAT CAAGCAGGAC AGCAACCTGA TTGGCCCGGA GGGAGGCGTG 
CTGAGCAGCA CCGTGGTGCC GCAGGTGCAG GCCGTGTTCC CCGAGGGGGC GCTAACCAAG 
CGGATCCGTG TTGGCCTACA GGCTCAACCT ATGCACAGTG AGTTGGTTAA GAAGATTCTG 
GGCAACAAAG CTACCTTCAG CCCTATAGTT ACTTTGGAAC CTAGAAGAAG AAAATTTCAC 
AAGCCAATTA CCATGACCAT TCCTGTCCCC AAAGCTTCAA GTGATGTCAT GTTGAATGGT 
TTTGGAGGAG ATGCACCAAC CTTAAGATTA CTATGTAGCA TCACAGGTGG AACCACCCCT 
GCTCAGTGGG AAGATATTAC AGGAACTACG CCATTAACAT TTGTCAATGA ATGTGTTTCC 
TTTACAACCA ATGTGTCTGC CAGGTTCTGG CTGATCGATT GTCGACAAAT TCAGGAATCT 
GTTACCTTTG CATCACAAGT GTATAGAGAA ATTATCTGTG TACCATATAT GGCCAAATTT 
GTAGTGTTTG CCAAATCACA TGACCCCATT GAAGCCAGGT TGAGGTGTTT CTGCATGACT 
GATGATAAAG TGGATAAAAC CCTGGAACAA CAAGAAAACT TTGCTGAGGT GGCCAGAAGC 
AGGGATGTGG AGGTCTTAGA AGGAAAACCC ATCTATGTTG ATTGTTTTGG CAACCTGGTG 
CCATTAACCA AGAGTGGCCA ACATCATATA TTCAGTTTTT TTGCCTTCAA AGAAAACAGA 
CTTCCTCTCT TTGTCAAGGT ACGTGATACG ACTCAGGAAC CTTGCGGACG ACTATCATTT 
ATGAAAGAGC CAAAATCTAC AAGAGGCCTG GTGCACCAAG CTATTTGCAA CTTAAACATC 
ACCCTGCCAA TTTATACGAA GGAATCAGAG TCAGATCAAG AACAGGAGGA AGAGATCGAT 
ATGACATCAG AAAAAAATCA ACAGGATGAG CAGGAACGGA TTGAGGAAAG GCTGGCATAT 
ATTGCTGATC ACCTTGGCTT CAGCTGGACA GAGTTGGCAA GAGAACTGGA CTTCACTGAA 
GAGCAAATTC ATCAAATTCG AATTGAGAAC CCTAACTCCC TGCAGGATCA GAGTCATGCG 
CTGTTGAAGT ACTGGCTAGA AAGGGACGGG AGACATGCTA CAGATACCAG TCTCGTTGAA 
TGTCTCACCA AGATCAACCG AATGGATATC GTGCATCTCA TGGAGACCAG CACAGAAGCT 
CTCCAGGAGC GCATCGGTCA TAGCTATGCA GAGATTGAGC AGACCATTAC ACTGGATCAT 
AGTGAAGGGT TCTCAGTACT CCAAGAGGAG CTTTGCACTG CCCAGCACAA GCAGAAAGAG 
GAGCATGCTG CTTCTAAAGA GGACGAGGCC TCTGACCACC CTCCCATCGT CTCAGAGGAA 
GACATTTCTG TTGGTTACTC CACTCTTCAG GATGGCATCC CCAAAACTGA GGGTGATGGC 
TCTGCAACAG AGCTCCTTCC CCAGACTCAA AAGGAGCAAG TTCAACAGGA TTTCTCAGGG 
AAAATGCAAG ACCTGCCTGA GGAGTCGGCT CTTGAACAGC AAGAATACTT CGTGACAACC 
CCAGGGACAG AAATGTCAGA GACTCAGAAG GCCACGGGAA CATCGGGCTC CCCCACCAAG 
ACACCTGAGG AAATTGTCAC CCCTTCCGAG GAGGAGAGGC TGTATCTCCA AACCCCAACA 
TCGAGTGAGC GGGGAGGCTC TCCAGTTGTG CAGGAACCTG AGGAGCCCCC CGAACGCAGG 
GAGGAGAGTT CACCTCGGAA AACCAGCCTC GTGATCGTGG AATCGGCTGA GGACCAGCCT 
CAGGCCTTCG AGAGGCTTGA TGAAGAGGCT GCTTTTCAAA AGGGAGATGA TATGCCTGAC 
ATACCCCCAG AAACAGTCAC AGAAGAAGAA TACATCGATG AGCATGGACA CACCGTGGTA 
AAGAAGGTTA CTAGGAAAAT CATTAGGCGG TTCATCTCCT CTGACGGCAC AGAGAAAGAG 
GAGATTACGA TGCAGGGAGC ACCACAGGAT TCTATCAGCG TTGATGAAGG GGATGGTTAT 
TCCAAAGTCA TAAAGCGCAT TGTATTGAAG AGTGACACCG AGCAGTCAGA GGTAACTTTG 
TCTGAGCCCA GCATTCTGTC CAGTACCTCA CAATTTCAGG CTGAACCAGT GGAAGGCCGT 
AGAGTCAGCA AAGTTGTTAA GACAACTGTG GTCCATGGTG AGAGGATGGA GAAACATCTG 
GGGGATTCTA GTTTAGCCAC TGATCTTCCT TCAGCCAAAG ATGACTTTGA AGAGGCTTTG 
AGTTACACAG GTAGCCACAT GAAAGTCTAC TCCCCCAGTT TAGTAGAGAA TGAAGTCCTG 
AAGGAGGATG GATCAATAAT TAGAAGGACG ACAATGAGTA AAGCTAGAAC CCAGAAGAGG 
GCTGTGGTGA AGGACCAGCA GGGAAAACGC ATTTACTTGG AGCACCTGGA GGATGTCCCA 
GAAGCGCTAG ACCAGGACGA CCTCCAGCAC GACCTCCAGC AACTCCTTCG GCATTTCTGC 
AAGGAGGACT CGAAGCAAGA GGCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK2 ( XM_024993495.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849263.1 Bos taurus ankyrin 2 (ANK2), transcript variant X11, mRNA.

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CloneID	OBa235487
Clone ID Related Accession (Same CDS sequence)	XM_024993495.1
Accession Version	XM_024993495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin-2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
ATGATGGTGA ATAGGACCAC CGAGAGTGGA TTCACCCCTT TGCACATCGC TGCCCACTAT 
GGAAACGTCA ACGTGGCAAC TCTCCTTCTA AACCGGGGAG CTGCTGTGGA CTTCACAGCC 
AGGAATGGAA TCACTCCTCT GCATGTGGCC TCCAAAAGGG GAAACACAAA CATGGTGAAG 
CTCTTACTGG ATCGTGGTGG TCAGATTGAT GCCAAAACTA GGGATGGGTT GACACCCCTT 
CATTGTGCTG CAAGAAGCGG TCATGACCAA GTGGTGGAGC TTCTGCTGGA GCGGGGCGCT 
CCCTTGCTGG CAAGGACGAA GAACGGGCTG TCTCCACTTC ACATGGCTGC ACAGGGGGAC 
CACGTGGAAT GTGTGAAGCA CCTGCTCCAG CACAAGGCGC CTGTTGATGA CGTCACTTTG 
GACTACTTGA CGGCCCTCCA CGTTGCCGCA CACTGCGGCC ACTACCGAGT AACCAAACTT 
CTTCTGGACA AGAGAGCCAA CCCGAACGCC AGAGCCCTGA ATGGTTTTAC TCCACTGCAC 
ATTGCCTGCA AGAAAAACCG CATCAAAGTC ATGGAACTGC TGGTGAAATA TGGGGCTTCA 
ATCCAAGCTA TAACAGAGTC TGGCCTCACA CCAATACATG TGGCTGCCTT CATGGGCCAC 
TTGAACATTG TCCTCCTTCT GCTGCAGAAC GGAGCCTCTC CAGATGTCAC TAACATTCGT 
GGGGAGACGG CCCTGCACAT GGCGGCCCGT GCAGGGCAGG TGGAAGTGGT CCGATGCCTC 
CTGAGGAACG GTGCCCTCGT CGATGCCAGA GCCAGGGAGG AACAGACGCC TTTGCATATT 
GCCTCCCGCC TGGGTAAGAC AGAAATTGTT CAGCTGCTTC TGCAACATAT GGCTCATCCA 
GATGCGGCCA CTACAAATGG GTACACGCCG TTGCATATCT CTGCAAGGGA AGGACAGGTG 
GACGTGGCCT CGGTCCTCCT GGAAGCGGGG GCAGCCCACT CCTTGGCTAC CAAGAAGGGT 
TTTACCCCCT TGCACGTAGC TGCCAAGTAT GGGAGCCTGG ATGTGGCAAA ACTTCTCTTG 
CAACGCCGTG CTGCCGCAGA TTCTGCGGGG AAGAATGGCC TTACCCCGCT CCATGTTGCT 
GCTCACTATG ACAACCAGAA GGTGGCGCTG CTGTTACTGG AGAAGGGTGC TTCCCCTCAC 
GCCATGGCCA AGAATGGCTA CACTCCTTTA CATATTGCTG CCAAGAAGAA TCAAATGCAG 
ATAGCTTCCA CACTCCTGAG CTACGGAGCA GAGACGAACA TCGTGACAAA GCAAGGAGTC 
ACTCCACTCC ATCTGGCCTC GCAGGAGGGA CACACGGATA TGGTCACCTT GCTTCTGGAT 
AAGGGAGCCA ATATCCACAT GTCAACCAAG AGTGGACTCA CATCTTTACA CCTTGCAGCC 
CAGGAAGATA AAGTGAATGT CGCTGATATT CTCACCAAGC ATGGGGCTGA CCAGGATGCG 
CACACAAAGC TTGGTTACAC TCCTTTAATT GTGGCCTGTC ACTATGGAAA CGTGAAAATG 
GTCAACTTCC TTCTGAAGCA GGGAGCAAAT GTCAATGCAA AAACCAAGAA TGGCTACACG 
CCTTTGCACC AAGCCGCCCA ACAGGGCCAC ACGCACATCA TCAATGTTTT GCTGCAGCAC 
GGGGCCAAGC CCAACGCTAC CACCGCGAAT GGCAACACTG CCTTGGCGAT TGCTAAGCGT 
CTGGGCTACA TCTCGGTGGT GGACACCCTG AAGGTTGTAA CGGAGGAGGT CACCACCACC 
ACCACGACTA TCACTGAAAA ACACAAGCTA AATGTTCCTG AGACCATGAC TGAGGTTCTT 
GATGTTTCTG ATGAAGAGGG TGATGACACA ATGACGGGTG ATGGGGGAGA GTACCTTAGA 
CCTGAGGACC TAAAGGAGCT GGGTGATGAC TCCCTCCCCA GCAGTCAGTT CCTGGACGGG 
ATGAATTACC TGCGCTACAG CTTGGAGGGG GGCCGCTCCG ACAGCCTTCG ATCCTTCAGT 
TCCGACAGGT CTCACACCCT GAGCCATGCC TCGTACCTGA GGGACAGTGC CATGATCGAC 
GACGCAGTTG TGATCCCCAG TCACCAGGTG ACAACTCTAG CCAAGGAGGC AGAAAGGAAT 
TCTTATCGTC TAAGCTGGGG CACTGAGAAC TTGGACAACG TGGCTCTTTC TTCCAGCCCT 
ATTCATTCAG GCCGCACCTC TCCATGTCTT GATCGTGACA ACAGCAGTTT CCTGGTTAGT 
TTTATGGTGG ATGCCCGAGG TGGTGCTATG CGAGGATGCA GACACAACGG GCTCCGCATC 
ATTATTCCGC CCCGGAAATG TACCGCCCCA ACACGAGTCA CCTGCCGACT GGTCAAACGC 
CATAGACTGG CGACAATGCC TCCAATGGTG GAAGGAGAGG GCCTGGCCAG TCGCCTGATT 
GAAGTTGGAC CTTCTGGTGC TCAGTTCCTT GGGCCTGTGA TCGTGGAGAT CCCTCATTTC 
GCTGCCCTGC GAGGAAAAGA GAGGGAACTG GTGGTCCTGC GCAGTGAGAA CGGGGACAGC 
TGGAAAGAGC ATTACTGTGA ATACACGGAG GAGGAGCTGA ATGAAATCCT GAACGGCATG 
GATGAAGTGC TGGATAGCCC GGAAGACCTC GAAAAGAAGC GGATCTGCCG CATCATCACC 
CGTGACTTCC CACAGTACTT TGCGGTGGTG TCGCGCATCA AGCAGGACAG CAACCTGATT 
GGCCCGGAGG GAGGCGTGCT GAGCAGCACC GTGGTGCCGC AGGTGCAGGC CGTGTTCCCC 
GAGGGGGCGC TAACCAAGCG GATCCGTGTT GGCCTACAGG CTCAACCTAT GCACAGTGAG 
TTGGTTAAGA AGATTCTGGG CAACAAAGCT ACCTTCAGCC CTATAGTTAC TTTGGAACCT 
AGAAGAAGAA AATTTCACAA GCCAATTACC ATGACCATTC CTGTCCCCAA AGCTTCAAGT 
GATGTCATGT TGAATGGTTT TGGAGGAGAT GCACCAACCT TAAGATTACT ATGTAGCATC 
ACAGGTGGAA CCACCCCTGC TCAGTGGGAA GATATTACAG GAACTACGCC ATTAACATTT 
GTCAATGAAT GTGTTTCCTT TACAACCAAT GTGTCTGCCA GGTTCTGGCT GATCGATTGT 
CGACAAATTC AGGAATCTGT TACCTTTGCA TCACAAGTGT ATAGAGAAAT TATCTGTGTA 
CCATATATGG CCAAATTTGT AGTGTTTGCC AAATCACATG ACCCCATTGA AGCCAGGTTG 
AGGTGTTTCT GCATGACTGA TGATAAAGTG GATAAAACCC TGGAACAACA AGAAAACTTT 
GCTGAGGTGG CCAGAAGCAG GGATGTGGAG GTCTTAGAAG GAAAACCCAT CTATGTTGAT 
TGTTTTGGCA ACCTGGTGCC ATTAACCAAG AGTGGCCAAC ATCATATATT CAGTTTTTTT 
GCCTTCAAAG AAAACAGACT TCCTCTCTTT GTCAAGGTAC GTGATACGAC TCAGGAACCT 
TGCGGACGAC TATCATTTAT GAAAGAGCCA AAATCTACAA GAGGCCTGGT GCACCAAGCT 
ATTTGCAACT TAAACATCAC CCTGCCAATT TATACGAAGG AATCAGAGTC AGATCAAGAA 
CAGGAGGAAG AGATCGATAT GACATCAGAA AAAAATCAAC AGGATGAGCA GGAACGGATT 
GAGGAAAGGC TGGCATATAT TGCTGATCAC CTTGGCTTCA GCTGGACAGA GTTGGCAAGA 
GAACTGGACT TCACTGAAGA GCAAATTCAT CAAATTCGAA TTGAGAACCC TAACTCCCTG 
CAGGATCAGA GTCATGCGCT GTTGAAGTAC TGGCTAGAAA GGGACGGGAG ACATGCTACA 
GATACCAGTC TCGTTGAATG TCTCACCAAG ATCAACCGAA TGGATATCGT GCATCTCATG 
GAGACCAGCA CAGAAGCTCT CCAGGAGCGC ATCGGTCATA GCTATGCAGA GATTGAGCAG 
ACCATTACAC TGGATCATAG TGAAGGGTTC TCAGTACTCC AAGAGGAGCT TTGCACTGCC 
CAGCACAAGC AGAAAGAGGA GCATGCTGCT TCTAAAGAGG ACGAGGCCTC TGACCACCCT 
CCCATCGTCT CAGAGGAAGA CATTTCTGTT GGTTACTCCA CTCTTCAGGA TGGCATCCCC 
AAAACTGAGG GTGATGGCTC TGCAACAGAG CTCCTTCCCC AGACTCAAAA GGAGCAAGTT 
CAACAGGATT TCTCAGGGAA AATGCAAGAC CTGCCTGAGG AGTCGGCTCT TGAACAGCAA 
GAATACTTCG TGACAACCCC AGGGACAGAA ATGTCAGAGA CTCAGAAGGC CACGGGAACA 
TCGGGCTCCC CCACCAAGAC ACCTGAGGAA ATTGTCACCC CTTCCGAGGA GGAGAGGCTG 
TATCTCCAAA CCCCAACATC GAGTGAGCGG GGAGGCTCTC CAGTTGTGCA GGAACCTGAG 
GAGCCCCCCG AACGCAGGGA GGAGAGTTCA CCTCGGAAAA CCAGCCTCGT GATCGTGGAA 
TCGGCTGAGG ACCAGCCTCA GGCCTTCGAG AGGCTTGATG AAGAGGCTGC TTTTCAAAAG 
GGAGATGATA TGCCTGACAT ACCCCCAGAA ACAGTCACAG AAGAAGAATA CATCGATGAG 
CATGGACACA CCGTGGTAAA GAAGGTTACT AGGAAAATCA TTAGGCGGTT CATCTCCTCT 
GACGGCACAG AGAAAGAGGA GATTACGATG CAGGGAGCAC CACAGGATTC TATCAGCGTT 
GATGAAGGGG ATGGTTATTC CAAAGTCATA AAGCGCATTG TATTGAAGAG TGACACCGAG 
CAGTCAGAGG TAACTTTGTC TGAGCCCAGC ATTCTGTCCA GTACCTCACA ATTTCAGGCT 
GAACCAGTGG AAGGCCGTAG AGTCAGCAAA GTTGTTAAGA CAACTGTGGT CCATGGTGAG 
AGGATGGAGA AACATCTGGG GGATTCTAGT TTAGCCACTG ATCTTCCTTC AGCCAAAGAT 
GACTTTGAAG AGGACGACAA TGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849263.1
CDS	239..6025
Translation	MGNAALCPACHADKSVRAQGHLQELDRTPDYYGCNGEDTKELGPRSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSAVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHAMAKNGYTPLHIAAKKNQMQIASTLLSYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMTEVLDVSDEEGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSHTLSHASYLRDSAMIDDAVVIPSHQVTTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGRTSPCLDRDNSSFLVSFMVDARGGAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHYCEYTEEELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEIDMTSEKNQQDEQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGRHATDTSLVECLTKINRMDIVHLMETSTEALQERIGHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEHAASKEDEASDHPPIVSEEDISVGYSTLQDGIPKTEGDGSATELLPQTQKEQVQQDFSGKMQDLPEESALEQQEYFVTTPGTEMSETQKATGTSGSPTKTPEEIVTPSEEERLYLQTPTSSERGGSPVVQEPEEPPERREESSPRKTSLVIVESAEDQPQAFERLDEEAAFQKGDDMPDIPPETVTEEEYIDEHGHTVVKKVTRKIIRRFISSDGTEKEEITMQGAPQDSISVDEGDGYSKVIKRIVLKSDTEQSEVTLSEPSILSSTSQFQAEPVEGRRVSKVVKTTVVHGERMEKHLGDSSLATDLPSAKDDFEEDDNE

Target ORF information:

RefSeq Version	XM_024993495.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin 2 (ANK2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993495.1

ORF Insert Sequence:

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
ATGATGGTGA ATAGGACCAC CGAGAGTGGA TTCACCCCTT TGCACATCGC TGCCCACTAT 
GGAAACGTCA ACGTGGCAAC TCTCCTTCTA AACCGGGGAG CTGCTGTGGA CTTCACAGCC 
AGGAATGGAA TCACTCCTCT GCATGTGGCC TCCAAAAGGG GAAACACAAA CATGGTGAAG 
CTCTTACTGG ATCGTGGTGG TCAGATTGAT GCCAAAACTA GGGATGGGTT GACACCCCTT 
CATTGTGCTG CAAGAAGCGG TCATGACCAA GTGGTGGAGC TTCTGCTGGA GCGGGGCGCT 
CCCTTGCTGG CAAGGACGAA GAACGGGCTG TCTCCACTTC ACATGGCTGC ACAGGGGGAC 
CACGTGGAAT GTGTGAAGCA CCTGCTCCAG CACAAGGCGC CTGTTGATGA CGTCACTTTG 
GACTACTTGA CGGCCCTCCA CGTTGCCGCA CACTGCGGCC ACTACCGAGT AACCAAACTT 
CTTCTGGACA AGAGAGCCAA CCCGAACGCC AGAGCCCTGA ATGGTTTTAC TCCACTGCAC 
ATTGCCTGCA AGAAAAACCG CATCAAAGTC ATGGAACTGC TGGTGAAATA TGGGGCTTCA 
ATCCAAGCTA TAACAGAGTC TGGCCTCACA CCAATACATG TGGCTGCCTT CATGGGCCAC 
TTGAACATTG TCCTCCTTCT GCTGCAGAAC GGAGCCTCTC CAGATGTCAC TAACATTCGT 
GGGGAGACGG CCCTGCACAT GGCGGCCCGT GCAGGGCAGG TGGAAGTGGT CCGATGCCTC 
CTGAGGAACG GTGCCCTCGT CGATGCCAGA GCCAGGGAGG AACAGACGCC TTTGCATATT 
GCCTCCCGCC TGGGTAAGAC AGAAATTGTT CAGCTGCTTC TGCAACATAT GGCTCATCCA 
GATGCGGCCA CTACAAATGG GTACACGCCG TTGCATATCT CTGCAAGGGA AGGACAGGTG 
GACGTGGCCT CGGTCCTCCT GGAAGCGGGG GCAGCCCACT CCTTGGCTAC CAAGAAGGGT 
TTTACCCCCT TGCACGTAGC TGCCAAGTAT GGGAGCCTGG ATGTGGCAAA ACTTCTCTTG 
CAACGCCGTG CTGCCGCAGA TTCTGCGGGG AAGAATGGCC TTACCCCGCT CCATGTTGCT 
GCTCACTATG ACAACCAGAA GGTGGCGCTG CTGTTACTGG AGAAGGGTGC TTCCCCTCAC 
GCCATGGCCA AGAATGGCTA CACTCCTTTA CATATTGCTG CCAAGAAGAA TCAAATGCAG 
ATAGCTTCCA CACTCCTGAG CTACGGAGCA GAGACGAACA TCGTGACAAA GCAAGGAGTC 
ACTCCACTCC ATCTGGCCTC GCAGGAGGGA CACACGGATA TGGTCACCTT GCTTCTGGAT 
AAGGGAGCCA ATATCCACAT GTCAACCAAG AGTGGACTCA CATCTTTACA CCTTGCAGCC 
CAGGAAGATA AAGTGAATGT CGCTGATATT CTCACCAAGC ATGGGGCTGA CCAGGATGCG 
CACACAAAGC TTGGTTACAC TCCTTTAATT GTGGCCTGTC ACTATGGAAA CGTGAAAATG 
GTCAACTTCC TTCTGAAGCA GGGAGCAAAT GTCAATGCAA AAACCAAGAA TGGCTACACG 
CCTTTGCACC AAGCCGCCCA ACAGGGCCAC ACGCACATCA TCAATGTTTT GCTGCAGCAC 
GGGGCCAAGC CCAACGCTAC CACCGCGAAT GGCAACACTG CCTTGGCGAT TGCTAAGCGT 
CTGGGCTACA TCTCGGTGGT GGACACCCTG AAGGTTGTAA CGGAGGAGGT CACCACCACC 
ACCACGACTA TCACTGAAAA ACACAAGCTA AATGTTCCTG AGACCATGAC TGAGGTTCTT 
GATGTTTCTG ATGAAGAGGG TGATGACACA ATGACGGGTG ATGGGGGAGA GTACCTTAGA 
CCTGAGGACC TAAAGGAGCT GGGTGATGAC TCCCTCCCCA GCAGTCAGTT CCTGGACGGG 
ATGAATTACC TGCGCTACAG CTTGGAGGGG GGCCGCTCCG ACAGCCTTCG ATCCTTCAGT 
TCCGACAGGT CTCACACCCT GAGCCATGCC TCGTACCTGA GGGACAGTGC CATGATCGAC 
GACGCAGTTG TGATCCCCAG TCACCAGGTG ACAACTCTAG CCAAGGAGGC AGAAAGGAAT 
TCTTATCGTC TAAGCTGGGG CACTGAGAAC TTGGACAACG TGGCTCTTTC TTCCAGCCCT 
ATTCATTCAG GCCGCACCTC TCCATGTCTT GATCGTGACA ACAGCAGTTT CCTGGTTAGT 
TTTATGGTGG ATGCCCGAGG TGGTGCTATG CGAGGATGCA GACACAACGG GCTCCGCATC 
ATTATTCCGC CCCGGAAATG TACCGCCCCA ACACGAGTCA CCTGCCGACT GGTCAAACGC 
CATAGACTGG CGACAATGCC TCCAATGGTG GAAGGAGAGG GCCTGGCCAG TCGCCTGATT 
GAAGTTGGAC CTTCTGGTGC TCAGTTCCTT GGGCCTGTGA TCGTGGAGAT CCCTCATTTC 
GCTGCCCTGC GAGGAAAAGA GAGGGAACTG GTGGTCCTGC GCAGTGAGAA CGGGGACAGC 
TGGAAAGAGC ATTACTGTGA ATACACGGAG GAGGAGCTGA ATGAAATCCT GAACGGCATG 
GATGAAGTGC TGGATAGCCC GGAAGACCTC GAAAAGAAGC GGATCTGCCG CATCATCACC 
CGTGACTTCC CACAGTACTT TGCGGTGGTG TCGCGCATCA AGCAGGACAG CAACCTGATT 
GGCCCGGAGG GAGGCGTGCT GAGCAGCACC GTGGTGCCGC AGGTGCAGGC CGTGTTCCCC 
GAGGGGGCGC TAACCAAGCG GATCCGTGTT GGCCTACAGG CTCAACCTAT GCACAGTGAG 
TTGGTTAAGA AGATTCTGGG CAACAAAGCT ACCTTCAGCC CTATAGTTAC TTTGGAACCT 
AGAAGAAGAA AATTTCACAA GCCAATTACC ATGACCATTC CTGTCCCCAA AGCTTCAAGT 
GATGTCATGT TGAATGGTTT TGGAGGAGAT GCACCAACCT TAAGATTACT ATGTAGCATC 
ACAGGTGGAA CCACCCCTGC TCAGTGGGAA GATATTACAG GAACTACGCC ATTAACATTT 
GTCAATGAAT GTGTTTCCTT TACAACCAAT GTGTCTGCCA GGTTCTGGCT GATCGATTGT 
CGACAAATTC AGGAATCTGT TACCTTTGCA TCACAAGTGT ATAGAGAAAT TATCTGTGTA 
CCATATATGG CCAAATTTGT AGTGTTTGCC AAATCACATG ACCCCATTGA AGCCAGGTTG 
AGGTGTTTCT GCATGACTGA TGATAAAGTG GATAAAACCC TGGAACAACA AGAAAACTTT 
GCTGAGGTGG CCAGAAGCAG GGATGTGGAG GTCTTAGAAG GAAAACCCAT CTATGTTGAT 
TGTTTTGGCA ACCTGGTGCC ATTAACCAAG AGTGGCCAAC ATCATATATT CAGTTTTTTT 
GCCTTCAAAG AAAACAGACT TCCTCTCTTT GTCAAGGTAC GTGATACGAC TCAGGAACCT 
TGCGGACGAC TATCATTTAT GAAAGAGCCA AAATCTACAA GAGGCCTGGT GCACCAAGCT 
ATTTGCAACT TAAACATCAC CCTGCCAATT TATACGAAGG AATCAGAGTC AGATCAAGAA 
CAGGAGGAAG AGATCGATAT GACATCAGAA AAAAATCAAC AGGATGAGCA GGAACGGATT 
GAGGAAAGGC TGGCATATAT TGCTGATCAC CTTGGCTTCA GCTGGACAGA GTTGGCAAGA 
GAACTGGACT TCACTGAAGA GCAAATTCAT CAAATTCGAA TTGAGAACCC TAACTCCCTG 
CAGGATCAGA GTCATGCGCT GTTGAAGTAC TGGCTAGAAA GGGACGGGAG ACATGCTACA 
GATACCAGTC TCGTTGAATG TCTCACCAAG ATCAACCGAA TGGATATCGT GCATCTCATG 
GAGACCAGCA CAGAAGCTCT CCAGGAGCGC ATCGGTCATA GCTATGCAGA GATTGAGCAG 
ACCATTACAC TGGATCATAG TGAAGGGTTC TCAGTACTCC AAGAGGAGCT TTGCACTGCC 
CAGCACAAGC AGAAAGAGGA GCATGCTGCT TCTAAAGAGG ACGAGGCCTC TGACCACCCT 
CCCATCGTCT CAGAGGAAGA CATTTCTGTT GGTTACTCCA CTCTTCAGGA TGGCATCCCC 
AAAACTGAGG GTGATGGCTC TGCAACAGAG CTCCTTCCCC AGACTCAAAA GGAGCAAGTT 
CAACAGGATT TCTCAGGGAA AATGCAAGAC CTGCCTGAGG AGTCGGCTCT TGAACAGCAA 
GAATACTTCG TGACAACCCC AGGGACAGAA ATGTCAGAGA CTCAGAAGGC CACGGGAACA 
TCGGGCTCCC CCACCAAGAC ACCTGAGGAA ATTGTCACCC CTTCCGAGGA GGAGAGGCTG 
TATCTCCAAA CCCCAACATC GAGTGAGCGG GGAGGCTCTC CAGTTGTGCA GGAACCTGAG 
GAGCCCCCCG AACGCAGGGA GGAGAGTTCA CCTCGGAAAA CCAGCCTCGT GATCGTGGAA 
TCGGCTGAGG ACCAGCCTCA GGCCTTCGAG AGGCTTGATG AAGAGGCTGC TTTTCAAAAG 
GGAGATGATA TGCCTGACAT ACCCCCAGAA ACAGTCACAG AAGAAGAATA CATCGATGAG 
CATGGACACA CCGTGGTAAA GAAGGTTACT AGGAAAATCA TTAGGCGGTT CATCTCCTCT 
GACGGCACAG AGAAAGAGGA GATTACGATG CAGGGAGCAC CACAGGATTC TATCAGCGTT 
GATGAAGGGG ATGGTTATTC CAAAGTCATA AAGCGCATTG TATTGAAGAG TGACACCGAG 
CAGTCAGAGG TAACTTTGTC TGAGCCCAGC ATTCTGTCCA GTACCTCACA ATTTCAGGCT 
GAACCAGTGG AAGGCCGTAG AGTCAGCAAA GTTGTTAAGA CAACTGTGGT CCATGGTGAG 
AGGATGGAGA AACATCTGGG GGATTCTAGT TTAGCCACTG ATCTTCCTTC AGCCAAAGAT 
GACTTTGAAG AGGACGACAA TGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK2 ( XM_024993496.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849264.1 Bos taurus ankyrin 2 (ANK2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa235488
Clone ID Related Accession (Same CDS sequence)	XM_024993496.1
Accession Version	XM_024993496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin-2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAACG AAGATGCAGC TCAGAAAAGC GACAGTGGAG AGAAGTTCAA TGGCAGTAGT 
CAGCGAAGGA AAAAACCCAA AAAGTCTGAC AGCAATGCAA GCTTCCTCCG TGCCGCCAGA 
GCAGGCAACT TAGACAAAGT TGTGGAATAT CTGAAGGGGG GCATAGACAT CAATACCTGC 
AACCAGAATG GACTCAACGC GCTTCACCTG GCTGCCAAGG AAGGCCACGT GGGGCTAGTT 
CAGGAGCTGC TGGGAAGAGG GTCTGCTGTG GACTCTGCCA CCAAGAAAGG AAATACTGCT 
CTTCACATTG CATCTTTGGC TGGACAAGCA GAAGTTGTCA AAGTTCTCGT CAAGGAAGGA 
GCCAATATTA ATGCACAGTC TCAGAATGGC TTCACTCCTT TATACATGGC TGCCCAAGAG 
AATCACATTG ATGTTGTAAA ATATTTGCTG GAAAATGGAG CTAATCAGAG CACCGCTACT 
GAGGATGGCT TTACGCCCCT GGCGGTGGCG CTCCAGCAGG GACACAACCA GGCAGTGGCC 
ATCCTCCTGG AGAACGACAC CAAAGGGAAG GTGCGGCTGC CAGCCCTGCA TATTGCAGCC 
AGGAAAGATG ACACCAAATC TGCCGCCCTC CTGCTTCAGA ACGATCACAA CGCTGACGTA 
CAGTCCAAGA GTGGATTCAC CCCTTTGCAC ATCGCTGCCC ACTATGGAAA CGTCAACGTG 
GCAACTCTCC TTCTAAACCG GGGAGCTGCT GTGGACTTCA CAGCCAGGAA TGGAATCACT 
CCTCTGCATG TGGCCTCCAA AAGGGGAAAC ACAAACATGG TGAAGCTCTT ACTGGATCGT 
GGTGGTCAGA TTGATGCCAA AACTAGGGAT GGGTTGACAC CCCTTCATTG TGCTGCAAGA 
AGCGGTCATG ACCAAGTGGT GGAGCTTCTG CTGGAGCGGG GCGCTCCCTT GCTGGCAAGG 
ACGAAGAACG GGCTGTCTCC ACTTCACATG GCTGCACAGG GGGACCACGT GGAATGTGTG 
AAGCACCTGC TCCAGCACAA GGCGCCTGTT GATGACGTCA CTTTGGACTA CTTGACGGCC 
CTCCACGTTG CCGCACACTG CGGCCACTAC CGAGTAACCA AACTTCTTCT GGACAAGAGA 
GCCAACCCGA ACGCCAGAGC CCTGAATGGT TTTACTCCAC TGCACATTGC CTGCAAGAAA 
AACCGCATCA AAGTCATGGA ACTGCTGGTG AAATATGGGG CTTCAATCCA AGCTATAACA 
GAGTCTGGCC TCACACCAAT ACATGTGGCT GCCTTCATGG GCCACTTGAA CATTGTCCTC 
CTTCTGCTGC AGAACGGAGC CTCTCCAGAT GTCACTAACA TTCGTGGGGA GACGGCCCTG 
CACATGGCGG CCCGTGCAGG GCAGGTGGAA GTGGTCCGAT GCCTCCTGAG GAACGGTGCC 
CTCGTCGATG CCAGAGCCAG GGAGGAACAG ACGCCTTTGC ATATTGCCTC CCGCCTGGGT 
AAGACAGAAA TTGTTCAGCT GCTTCTGCAA CATATGGCTC ATCCAGATGC GGCCACTACA 
AATGGGTACA CGCCGTTGCA TATCTCTGCA AGGGAAGGAC AGGTGGACGT GGCCTCGGTC 
CTCCTGGAAG CGGGGGCAGC CCACTCCTTG GCTACCAAGA AGGGTTTTAC CCCCTTGCAC 
GTAGCTGCCA AGTATGGGAG CCTGGATGTG GCAAAACTTC TCTTGCAACG CCGTGCTGCC 
GCAGATTCTG CGGGGAAGAA TGGCTACACT CCTTTACATA TTGCTGCCAA GAAGAATCAA 
ATGCAGATAG CTTCCACACT CCTGAGCTAC GGAGCAGAGA CGAACATCGT GACAAAGCAA 
GGAGTCACTC CACTCCATCT GGCCTCGCAG GAGGGACACA CGGATATGGT CACCTTGCTT 
CTGGATAAGG GAGCCAATAT CCACATGTCA ACCAAGAGTG GACTCACATC TTTACACCTT 
GCAGCCCAGG AAGATAAAGT GAATGTCGCT GATATTCTCA CCAAGCATGG GGCTGACCAG 
GATGCGCACA CAAAGCTTGG TTACACTCCT TTAATTGTGG CCTGTCACTA TGGAAACGTG 
AAAATGGTCA ACTTCCTTCT GAAGCAGGGA GCAAATGTCA ATGCAAAAAC CAAGAATGGC 
TACACGCCTT TGCACCAAGC CGCCCAACAG GGCCACACGC ACATCATCAA TGTTTTGCTG 
CAGCACGGGG CCAAGCCCAA CGCTACCACC GCGAATGGCA ACACTGCCTT GGCGATTGCT 
AAGCGTCTGG GCTACATCTC GGTGGTGGAC ACCCTGAAGG TTGTAACGGA GGAGGTCACC 
ACCACCACCA CGACTATCAC TGAAAAACAC AAGCTAAATG TTCCTGAGAC CATGACTGAG 
GTTCTTGATG TTTCTGATGA AGAGGGTGAT GACACAATGA CGGGTGATGG GGGAGAGTAC 
CTTAGACCTG AGGACCTAAA GGAGCTGGGT GATGACTCCC TCCCCAGCAG TCAGTTCCTG 
GACGGGATGA ATTACCTGCG CTACAGCTTG GAGGGGGGCC GCTCCGACAG CCTTCGATCC 
TTCAGTTCCG ACAGGTCTCA CACCCTGAGC CATGCCTCGT ACCTGAGGGA CAGTGCCATG 
ATCGACGACG CAGTTGTGAT CCCCAGTCAC CAGGTGACAA CTCTAGCCAA GGAGGCAGAA 
AGGAATTCTT ATCGTCTAAG CTGGGGCACT GAGAACTTGG ACAACGTGGC TCTTTCTTCC 
AGCCCTATTC ATTCAGGCCG CACCTCTCCA TGTCTTGATC GTGACAACAG CAGTTTCCTG 
GTTAGTTTTA TGGTGGATGC CCGAGGTGGT GCTATGCGAG GATGCAGACA CAACGGGCTC 
CGCATCATTA TTCCGCCCCG GAAATGTACC GCCCCAACAC GAGTCACCTG CCGACTGGTC 
AAACGCCATA GACTGGCGAC AATGCCTCCA ATGGTGGAAG GAGAGGGCCT GGCCAGTCGC 
CTGATTGAAG TTGGACCTTC TGGTGCTCAG TTCCTTGGGC CTGTGATCGT GGAGATCCCT 
CATTTCGCTG CCCTGCGAGG AAAAGAGAGG GAACTGGTGG TCCTGCGCAG TGAGAACGGG 
GACAGCTGGA AAGAGCATTA CTGTGAATAC ACGGAGGAGG AGCTGAATGA AATCCTGAAC 
GGCATGGATG AAGTGCTGGA TAGCCCGGAA GACCTCGAAA AGAAGCGGAT CTGCCGCATC 
ATCACCCGTG ACTTCCCACA GTACTTTGCG GTGGTGTCGC GCATCAAGCA GGACAGCAAC 
CTGATTGGCC CGGAGGGAGG CGTGCTGAGC AGCACCGTGG TGCCGCAGGT GCAGGCCGTG 
TTCCCCGAGG GGGCGCTAAC CAAGCGGATC CGTGTTGGCC TACAGGCTCA ACCTATGCAC 
AGTGAGTTGG TTAAGAAGAT TCTGGGCAAC AAAGCTACCT TCAGCCCTAT AGTTACTTTG 
GAACCTAGAA GAAGAAAATT TCACAAGCCA ATTACCATGA CCATTCCTGT CCCCAAAGCT 
TCAAGTGATG TCATGTTGAA TGGTTTTGGA GGAGATGCAC CAACCTTAAG ATTACTATGT 
AGCATCACAG GTGGAACCAC CCCTGCTCAG TGGGAAGATA TTACAGGAAC TACGCCATTA 
ACATTTGTCA ATGAATGTGT TTCCTTTACA ACCAATGTGT CTGCCAGGTT CTGGCTGATC 
GATTGTCGAC AAATTCAGGA ATCTGTTACC TTTGCATCAC AAGTGTATAG AGAAATTATC 
TGTGTACCAT ATATGGCCAA ATTTGTAGTG TTTGCCAAAT CACATGACCC CATTGAAGCC 
AGGTTGAGGT GTTTCTGCAT GACTGATGAT AAAGTGGATA AAACCCTGGA ACAACAAGAA 
AACTTTGCTG AGGTGGCCAG AAGCAGGGAT GTGGAGGTCT TAGAAGGAAA ACCCATCTAT 
GTTGATTGTT TTGGCAACCT GGTGCCATTA ACCAAGAGTG GCCAACATCA TATATTCAGT 
TTTTTTGCCT TCAAAGAAAA CAGACTTCCT CTCTTTGTCA AGGTACGTGA TACGACTCAG 
GAACCTTGCG GACGACTATC ATTTATGAAA GAGCCAAAAT CTACAAGAGG CCTGGTGCAC 
CAAGCTATTT GCAACTTAAA CATCACCCTG CCAATTTATA CGAAGGAATC AGAGTCAGAT 
CAAGAACAGG AGGAAGAGAT CGATATGACA TCAGAAAAAA ATCAACAGGA TGAGCAGGAA 
CGGATTGAGG AAAGGCTGGC ATATATTGCT GATCACCTTG GCTTCAGCTG GACAGAGTTG 
GCAAGAGAAC TGGACTTCAC TGAAGAGCAA ATTCATCAAA TTCGAATTGA GAACCCTAAC 
TCCCTGCAGG ATCAGAGTCA TGCGCTGTTG AAGTACTGGC TAGAAAGGGA CGGGAGACAT 
GCTACAGATA CCAGTCTCGT TGAATGTCTC ACCAAGATCA ACCGAATGGA TATCGTGCAT 
CTCATGGAGA CCAGCACAGA AGCTCTCCAG GAGCGCATCG GTCATAGCTA TGCAGAGATT 
GAGCAGACCA TTACACTGGA TCATAGTGAA GGGTTCTCAG TACTCCAAGA GGAGCTTTGC 
ACTGCCCAGC ACAAGCAGAA AGAGGAGCAT GCTGCTTCTA AAGAGGACGA GGCCTCTGAC 
CACCCTCCCA TCGTCTCAGA GGAAGACATT TCTGTTGGTT ACTCCACTCT TCAGGATGGC 
ATCCCCAAAA CTGAGGGTGA TGGCTCTGCA ACAGAGCTCC TTCCCCAGAC TCAAAAGGAG 
CAAGTTCAAC AGGATTTCTC AGGGAAAATG CAAGACCTGC CTGAGGAGTC GGCTCTTGAA 
CAGCAAGAAT ACTTCGTGAC AACCCCAGGG ACAGAAATGT CAGAGACTCA GAAGGCCACG 
GGAACATCGG GCTCCCCCAC CAAGACACCT GAGGAAATTG TCACCCCTTC CGAGGAGGAG 
AGGCTGTATC TCCAAACCCC AACATCGAGT GAGCGGGGAG GCTCTCCAGT TGTGCAGGAA 
CCTGAGGAGC CCCCCGAACG CAGGGAGGAG AGTTCACCTC GGAAAACCAG CCTCGTGATC 
GTGGAATCGG CTGAGGACCA GCCTCAGGCC TTCGAGAGGC TTGATGAAGA GGCTGCTTTT 
CAAAAGGGAG ATGATATGCC TGACATACCC CCAGAAACAG TCACAGAAGA AGAATACATC 
GATGAGCATG GACACACCGT GGTAAAGAAG GTTACTAGGA AAATCATTAG GCGGTTCATC 
TCCTCTGACG GCACAGAGAA AGAGGAGATT ACGATGCAGG GAGCACCACA GGATTCTATC 
AGCGTTGATG AAGGGGATGG TTATTCCAAA GTCATAAAGC GCATTGTATT GAAGAGTGAC 
ACCGAGCAGT CAGAGGTAAC TTTGTCTGAG CCCAGCATTC TGTCCAGTAC CTCACAATTT 
CAGGCTGAAC CAGTGGAAGG CCGTAGAGTC AGCAAAGTTG TTAAGACAAC TGTGGTCCAT 
GGTGAGAGGA TGGAGAAACA TCTGGGGGAT TCTAGTTTAG CCACTGATCT TCCTTCAGCC 
AAAGATGACT TTGAAGAGGA CGACAATGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849264.1
CDS	97..5709
Translation	MMNEDAAQKSDSGEKFNGSSQRRKKPKKSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSAVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQIASTLLSYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMTEVLDVSDEEGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSHTLSHASYLRDSAMIDDAVVIPSHQVTTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGRTSPCLDRDNSSFLVSFMVDARGGAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHYCEYTEEELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEIDMTSEKNQQDEQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGRHATDTSLVECLTKINRMDIVHLMETSTEALQERIGHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEHAASKEDEASDHPPIVSEEDISVGYSTLQDGIPKTEGDGSATELLPQTQKEQVQQDFSGKMQDLPEESALEQQEYFVTTPGTEMSETQKATGTSGSPTKTPEEIVTPSEEERLYLQTPTSSERGGSPVVQEPEEPPERREESSPRKTSLVIVESAEDQPQAFERLDEEAAFQKGDDMPDIPPETVTEEEYIDEHGHTVVKKVTRKIIRRFISSDGTEKEEITMQGAPQDSISVDEGDGYSKVIKRIVLKSDTEQSEVTLSEPSILSSTSQFQAEPVEGRRVSKVVKTTVVHGERMEKHLGDSSLATDLPSAKDDFEEDDNE

Target ORF information:

RefSeq Version	XM_024993496.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin 2 (ANK2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993496.1

ORF Insert Sequence:

ATGATGAACG AAGATGCAGC TCAGAAAAGC GACAGTGGAG AGAAGTTCAA TGGCAGTAGT 
CAGCGAAGGA AAAAACCCAA AAAGTCTGAC AGCAATGCAA GCTTCCTCCG TGCCGCCAGA 
GCAGGCAACT TAGACAAAGT TGTGGAATAT CTGAAGGGGG GCATAGACAT CAATACCTGC 
AACCAGAATG GACTCAACGC GCTTCACCTG GCTGCCAAGG AAGGCCACGT GGGGCTAGTT 
CAGGAGCTGC TGGGAAGAGG GTCTGCTGTG GACTCTGCCA CCAAGAAAGG AAATACTGCT 
CTTCACATTG CATCTTTGGC TGGACAAGCA GAAGTTGTCA AAGTTCTCGT CAAGGAAGGA 
GCCAATATTA ATGCACAGTC TCAGAATGGC TTCACTCCTT TATACATGGC TGCCCAAGAG 
AATCACATTG ATGTTGTAAA ATATTTGCTG GAAAATGGAG CTAATCAGAG CACCGCTACT 
GAGGATGGCT TTACGCCCCT GGCGGTGGCG CTCCAGCAGG GACACAACCA GGCAGTGGCC 
ATCCTCCTGG AGAACGACAC CAAAGGGAAG GTGCGGCTGC CAGCCCTGCA TATTGCAGCC 
AGGAAAGATG ACACCAAATC TGCCGCCCTC CTGCTTCAGA ACGATCACAA CGCTGACGTA 
CAGTCCAAGA GTGGATTCAC CCCTTTGCAC ATCGCTGCCC ACTATGGAAA CGTCAACGTG 
GCAACTCTCC TTCTAAACCG GGGAGCTGCT GTGGACTTCA CAGCCAGGAA TGGAATCACT 
CCTCTGCATG TGGCCTCCAA AAGGGGAAAC ACAAACATGG TGAAGCTCTT ACTGGATCGT 
GGTGGTCAGA TTGATGCCAA AACTAGGGAT GGGTTGACAC CCCTTCATTG TGCTGCAAGA 
AGCGGTCATG ACCAAGTGGT GGAGCTTCTG CTGGAGCGGG GCGCTCCCTT GCTGGCAAGG 
ACGAAGAACG GGCTGTCTCC ACTTCACATG GCTGCACAGG GGGACCACGT GGAATGTGTG 
AAGCACCTGC TCCAGCACAA GGCGCCTGTT GATGACGTCA CTTTGGACTA CTTGACGGCC 
CTCCACGTTG CCGCACACTG CGGCCACTAC CGAGTAACCA AACTTCTTCT GGACAAGAGA 
GCCAACCCGA ACGCCAGAGC CCTGAATGGT TTTACTCCAC TGCACATTGC CTGCAAGAAA 
AACCGCATCA AAGTCATGGA ACTGCTGGTG AAATATGGGG CTTCAATCCA AGCTATAACA 
GAGTCTGGCC TCACACCAAT ACATGTGGCT GCCTTCATGG GCCACTTGAA CATTGTCCTC 
CTTCTGCTGC AGAACGGAGC CTCTCCAGAT GTCACTAACA TTCGTGGGGA GACGGCCCTG 
CACATGGCGG CCCGTGCAGG GCAGGTGGAA GTGGTCCGAT GCCTCCTGAG GAACGGTGCC 
CTCGTCGATG CCAGAGCCAG GGAGGAACAG ACGCCTTTGC ATATTGCCTC CCGCCTGGGT 
AAGACAGAAA TTGTTCAGCT GCTTCTGCAA CATATGGCTC ATCCAGATGC GGCCACTACA 
AATGGGTACA CGCCGTTGCA TATCTCTGCA AGGGAAGGAC AGGTGGACGT GGCCTCGGTC 
CTCCTGGAAG CGGGGGCAGC CCACTCCTTG GCTACCAAGA AGGGTTTTAC CCCCTTGCAC 
GTAGCTGCCA AGTATGGGAG CCTGGATGTG GCAAAACTTC TCTTGCAACG CCGTGCTGCC 
GCAGATTCTG CGGGGAAGAA TGGCTACACT CCTTTACATA TTGCTGCCAA GAAGAATCAA 
ATGCAGATAG CTTCCACACT CCTGAGCTAC GGAGCAGAGA CGAACATCGT GACAAAGCAA 
GGAGTCACTC CACTCCATCT GGCCTCGCAG GAGGGACACA CGGATATGGT CACCTTGCTT 
CTGGATAAGG GAGCCAATAT CCACATGTCA ACCAAGAGTG GACTCACATC TTTACACCTT 
GCAGCCCAGG AAGATAAAGT GAATGTCGCT GATATTCTCA CCAAGCATGG GGCTGACCAG 
GATGCGCACA CAAAGCTTGG TTACACTCCT TTAATTGTGG CCTGTCACTA TGGAAACGTG 
AAAATGGTCA ACTTCCTTCT GAAGCAGGGA GCAAATGTCA ATGCAAAAAC CAAGAATGGC 
TACACGCCTT TGCACCAAGC CGCCCAACAG GGCCACACGC ACATCATCAA TGTTTTGCTG 
CAGCACGGGG CCAAGCCCAA CGCTACCACC GCGAATGGCA ACACTGCCTT GGCGATTGCT 
AAGCGTCTGG GCTACATCTC GGTGGTGGAC ACCCTGAAGG TTGTAACGGA GGAGGTCACC 
ACCACCACCA CGACTATCAC TGAAAAACAC AAGCTAAATG TTCCTGAGAC CATGACTGAG 
GTTCTTGATG TTTCTGATGA AGAGGGTGAT GACACAATGA CGGGTGATGG GGGAGAGTAC 
CTTAGACCTG AGGACCTAAA GGAGCTGGGT GATGACTCCC TCCCCAGCAG TCAGTTCCTG 
GACGGGATGA ATTACCTGCG CTACAGCTTG GAGGGGGGCC GCTCCGACAG CCTTCGATCC 
TTCAGTTCCG ACAGGTCTCA CACCCTGAGC CATGCCTCGT ACCTGAGGGA CAGTGCCATG 
ATCGACGACG CAGTTGTGAT CCCCAGTCAC CAGGTGACAA CTCTAGCCAA GGAGGCAGAA 
AGGAATTCTT ATCGTCTAAG CTGGGGCACT GAGAACTTGG ACAACGTGGC TCTTTCTTCC 
AGCCCTATTC ATTCAGGCCG CACCTCTCCA TGTCTTGATC GTGACAACAG CAGTTTCCTG 
GTTAGTTTTA TGGTGGATGC CCGAGGTGGT GCTATGCGAG GATGCAGACA CAACGGGCTC 
CGCATCATTA TTCCGCCCCG GAAATGTACC GCCCCAACAC GAGTCACCTG CCGACTGGTC 
AAACGCCATA GACTGGCGAC AATGCCTCCA ATGGTGGAAG GAGAGGGCCT GGCCAGTCGC 
CTGATTGAAG TTGGACCTTC TGGTGCTCAG TTCCTTGGGC CTGTGATCGT GGAGATCCCT 
CATTTCGCTG CCCTGCGAGG AAAAGAGAGG GAACTGGTGG TCCTGCGCAG TGAGAACGGG 
GACAGCTGGA AAGAGCATTA CTGTGAATAC ACGGAGGAGG AGCTGAATGA AATCCTGAAC 
GGCATGGATG AAGTGCTGGA TAGCCCGGAA GACCTCGAAA AGAAGCGGAT CTGCCGCATC 
ATCACCCGTG ACTTCCCACA GTACTTTGCG GTGGTGTCGC GCATCAAGCA GGACAGCAAC 
CTGATTGGCC CGGAGGGAGG CGTGCTGAGC AGCACCGTGG TGCCGCAGGT GCAGGCCGTG 
TTCCCCGAGG GGGCGCTAAC CAAGCGGATC CGTGTTGGCC TACAGGCTCA ACCTATGCAC 
AGTGAGTTGG TTAAGAAGAT TCTGGGCAAC AAAGCTACCT TCAGCCCTAT AGTTACTTTG 
GAACCTAGAA GAAGAAAATT TCACAAGCCA ATTACCATGA CCATTCCTGT CCCCAAAGCT 
TCAAGTGATG TCATGTTGAA TGGTTTTGGA GGAGATGCAC CAACCTTAAG ATTACTATGT 
AGCATCACAG GTGGAACCAC CCCTGCTCAG TGGGAAGATA TTACAGGAAC TACGCCATTA 
ACATTTGTCA ATGAATGTGT TTCCTTTACA ACCAATGTGT CTGCCAGGTT CTGGCTGATC 
GATTGTCGAC AAATTCAGGA ATCTGTTACC TTTGCATCAC AAGTGTATAG AGAAATTATC 
TGTGTACCAT ATATGGCCAA ATTTGTAGTG TTTGCCAAAT CACATGACCC CATTGAAGCC 
AGGTTGAGGT GTTTCTGCAT GACTGATGAT AAAGTGGATA AAACCCTGGA ACAACAAGAA 
AACTTTGCTG AGGTGGCCAG AAGCAGGGAT GTGGAGGTCT TAGAAGGAAA ACCCATCTAT 
GTTGATTGTT TTGGCAACCT GGTGCCATTA ACCAAGAGTG GCCAACATCA TATATTCAGT 
TTTTTTGCCT TCAAAGAAAA CAGACTTCCT CTCTTTGTCA AGGTACGTGA TACGACTCAG 
GAACCTTGCG GACGACTATC ATTTATGAAA GAGCCAAAAT CTACAAGAGG CCTGGTGCAC 
CAAGCTATTT GCAACTTAAA CATCACCCTG CCAATTTATA CGAAGGAATC AGAGTCAGAT 
CAAGAACAGG AGGAAGAGAT CGATATGACA TCAGAAAAAA ATCAACAGGA TGAGCAGGAA 
CGGATTGAGG AAAGGCTGGC ATATATTGCT GATCACCTTG GCTTCAGCTG GACAGAGTTG 
GCAAGAGAAC TGGACTTCAC TGAAGAGCAA ATTCATCAAA TTCGAATTGA GAACCCTAAC 
TCCCTGCAGG ATCAGAGTCA TGCGCTGTTG AAGTACTGGC TAGAAAGGGA CGGGAGACAT 
GCTACAGATA CCAGTCTCGT TGAATGTCTC ACCAAGATCA ACCGAATGGA TATCGTGCAT 
CTCATGGAGA CCAGCACAGA AGCTCTCCAG GAGCGCATCG GTCATAGCTA TGCAGAGATT 
GAGCAGACCA TTACACTGGA TCATAGTGAA GGGTTCTCAG TACTCCAAGA GGAGCTTTGC 
ACTGCCCAGC ACAAGCAGAA AGAGGAGCAT GCTGCTTCTA AAGAGGACGA GGCCTCTGAC 
CACCCTCCCA TCGTCTCAGA GGAAGACATT TCTGTTGGTT ACTCCACTCT TCAGGATGGC 
ATCCCCAAAA CTGAGGGTGA TGGCTCTGCA ACAGAGCTCC TTCCCCAGAC TCAAAAGGAG 
CAAGTTCAAC AGGATTTCTC AGGGAAAATG CAAGACCTGC CTGAGGAGTC GGCTCTTGAA 
CAGCAAGAAT ACTTCGTGAC AACCCCAGGG ACAGAAATGT CAGAGACTCA GAAGGCCACG 
GGAACATCGG GCTCCCCCAC CAAGACACCT GAGGAAATTG TCACCCCTTC CGAGGAGGAG 
AGGCTGTATC TCCAAACCCC AACATCGAGT GAGCGGGGAG GCTCTCCAGT TGTGCAGGAA 
CCTGAGGAGC CCCCCGAACG CAGGGAGGAG AGTTCACCTC GGAAAACCAG CCTCGTGATC 
GTGGAATCGG CTGAGGACCA GCCTCAGGCC TTCGAGAGGC TTGATGAAGA GGCTGCTTTT 
CAAAAGGGAG ATGATATGCC TGACATACCC CCAGAAACAG TCACAGAAGA AGAATACATC 
GATGAGCATG GACACACCGT GGTAAAGAAG GTTACTAGGA AAATCATTAG GCGGTTCATC 
TCCTCTGACG GCACAGAGAA AGAGGAGATT ACGATGCAGG GAGCACCACA GGATTCTATC 
AGCGTTGATG AAGGGGATGG TTATTCCAAA GTCATAAAGC GCATTGTATT GAAGAGTGAC 
ACCGAGCAGT CAGAGGTAAC TTTGTCTGAG CCCAGCATTC TGTCCAGTAC CTCACAATTT 
CAGGCTGAAC CAGTGGAAGG CCGTAGAGTC AGCAAAGTTG TTAAGACAAC TGTGGTCCAT 
GGTGAGAGGA TGGAGAAACA TCTGGGGGAT TCTAGTTTAG CCACTGATCT TCCTTCAGCC 
AAAGATGACT TTGAAGAGGA CGACAATGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK2 ( XM_024993497.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849265.1 Bos taurus ankyrin 2 (ANK2), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa235489
Clone ID Related Accession (Same CDS sequence)	XM_024993497.1
Accession Version	XM_024993497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin-2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACCA TGTTGCAAAA GTCTGACAGC AATGCAAGCT TCCTCCGTGC CGCCAGAGCA 
GGCAACTTAG ACAAAGTTGT GGAATATCTG AAGGGGGGCA TAGACATCAA TACCTGCAAC 
CAGAATGGAC TCAACGCGCT TCACCTGGCT GCCAAGGAAG GCCACGTGGG GCTAGTTCAG 
GAGCTGCTGG GAAGAGGGTC TGCTGTGGAC TCTGCCACCA AGAAAGGAAA TACTGCTCTT 
CACATTGCAT CTTTGGCTGG ACAAGCAGAA GTTGTCAAAG TTCTCGTCAA GGAAGGAGCC 
AATATTAATG CACAGTCTCA GAATGGCTTC ACTCCTTTAT ACATGGCTGC CCAAGAGAAT 
CACATTGATG TTGTAAAATA TTTGCTGGAA AATGGAGCTA ATCAGAGCAC CGCTACTGAG 
GATGGCTTTA CGCCCCTGGC GGTGGCGCTC CAGCAGGGAC ACAACCAGGC AGTGGCCATC 
CTCCTGGAGA ACGACACCAA AGGGAAGGTG CGGCTGCCAG CCCTGCATAT TGCAGCCAGG 
AAAGATGACA CCAAATCTGC CGCCCTCCTG CTTCAGAACG ATCACAACGC TGACGTACAG 
TCCAAGATGA TGGTGAATAG GACCACCGAG AGTGGATTCA CCCCTTTGCA CATCGCTGCC 
CACTATGGAA ACGTCAACGT GGCAACTCTC CTTCTAAACC GGGGAGCTGC TGTGGACTTC 
ACAGCCAGGA ATGGAATCAC TCCTCTGCAT GTGGCCTCCA AAAGGGGAAA CACAAACATG 
GTGAAGCTCT TACTGGATCG TGGTGGTCAG ATTGATGCCA AAACTAGGGA TGGGTTGACA 
CCCCTTCATT GTGCTGCAAG AAGCGGTCAT GACCAAGTGG TGGAGCTTCT GCTGGAGCGG 
GGCGCTCCCT TGCTGGCAAG GACGAAGAAC GGGCTGTCTC CACTTCACAT GGCTGCACAG 
GGGGACCACG TGGAATGTGT GAAGCACCTG CTCCAGCACA AGGCGCCTGT TGATGACGTC 
ACTTTGGACT ACTTGACGGC CCTCCACGTT GCCGCACACT GCGGCCACTA CCGAGTAACC 
AAACTTCTTC TGGACAAGAG AGCCAACCCG AACGCCAGAG CCCTGAATGG TTTTACTCCA 
CTGCACATTG CCTGCAAGAA AAACCGCATC AAAGTCATGG AACTGCTGGT GAAATATGGG 
GCTTCAATCC AAGCTATAAC AGAGTCTGGC CTCACACCAA TACATGTGGC TGCCTTCATG 
GGCCACTTGA ACATTGTCCT CCTTCTGCTG CAGAACGGAG CCTCTCCAGA TGTCACTAAC 
ATTCGTGGGG AGACGGCCCT GCACATGGCG GCCCGTGCAG GGCAGGTGGA AGTGGTCCGA 
TGCCTCCTGA GGAACGGTGC CCTCGTCGAT GCCAGAGCCA GGGAGGAACA GACGCCTTTG 
CATATTGCCT CCCGCCTGGG TAAGACAGAA ATTGTTCAGC TGCTTCTGCA ACATATGGCT 
CATCCAGATG CGGCCACTAC AAATGGGTAC ACGCCGTTGC ATATCTCTGC AAGGGAAGGA 
CAGGTGGACG TGGCCTCGGT CCTCCTGGAA GCGGGGGCAG CCCACTCCTT GGCTACCAAG 
AAGGGTTTTA CCCCCTTGCA CGTAGCTGCC AAGTATGGGA GCCTGGATGT GGCAAAACTT 
CTCTTGCAAC GCCGTGCTGC CGCAGATTCT GCGGGGAAGA ATGGCTACAC TCCTTTACAT 
ATTGCTGCCA AGAAGAATCA AATGCAGATA GCTTCCACAC TCCTGAGCTA CGGAGCAGAG 
ACGAACATCG TGACAAAGCA AGGAGTCACT CCACTCCATC TGGCCTCGCA GGAGGGACAC 
ACGGATATGG TCACCTTGCT TCTGGATAAG GGAGCCAATA TCCACATGTC AACCAAGAGT 
GGACTCACAT CTTTACACCT TGCAGCCCAG GAAGATAAAG TGAATGTCGC TGATATTCTC 
ACCAAGCATG GGGCTGACCA GGATGCGCAC ACAAAGCTTG GTTACACTCC TTTAATTGTG 
GCCTGTCACT ATGGAAACGT GAAAATGGTC AACTTCCTTC TGAAGCAGGG AGCAAATGTC 
AATGCAAAAA CCAAGAATGG CTACACGCCT TTGCACCAAG CCGCCCAACA GGGCCACACG 
CACATCATCA ATGTTTTGCT GCAGCACGGG GCCAAGCCCA ACGCTACCAC CGCGAATGGC 
AACACTGCCT TGGCGATTGC TAAGCGTCTG GGCTACATCT CGGTGGTGGA CACCCTGAAG 
GTTGTAACGG AGGAGGTCAC CACCACCACC ACGACTATCA CTGAAAAACA CAAGCTAAAT 
GTTCCTGAGA CCATGACTGA GGTTCTTGAT GTTTCTGATG AAGAGGGTGA TGACACAATG 
ACGGGTGATG GGGGAGAGTA CCTTAGACCT GAGGACCTAA AGGAGCTGGG TGATGACTCC 
CTCCCCAGCA GTCAGTTCCT GGACGGGATG AATTACCTGC GCTACAGCTT GGAGGGGGGC 
CGCTCCGACA GCCTTCGATC CTTCAGTTCC GACAGGTCTC ACACCCTGAG CCATGCCTCG 
TACCTGAGGG ACAGTGCCAT GATCGACGAC GCAGTTGTGA TCCCCAGTCA CCAGGTGACA 
ACTCTAGCCA AGGAGGCAGA AAGGAATTCT TATCGTCTAA GCTGGGGCAC TGAGAACTTG 
GACAACGTGG CTCTTTCTTC CAGCCCTATT CATTCAGGCC GCACCTCTCC ATGTCTTGAT 
CGTGACAACA GCAGTTTCCT GGTTAGTTTT ATGGTGGATG CCCGAGGTGG TGCTATGCGA 
GGATGCAGAC ACAACGGGCT CCGCATCATT ATTCCGCCCC GGAAATGTAC CGCCCCAACA 
CGAGTCACCT GCCGACTGGT CAAACGCCAT AGACTGGCGA CAATGCCTCC AATGGTGGAA 
GGAGAGGGCC TGGCCAGTCG CCTGATTGAA GTTGGACCTT CTGGTGCTCA GTTCCTTGGG 
CCTGTGATCG TGGAGATCCC TCATTTCGCT GCCCTGCGAG GAAAAGAGAG GGAACTGGTG 
GTCCTGCGCA GTGAGAACGG GGACAGCTGG AAAGAGCATT ACTGTGAATA CACGGAGGAG 
GAGCTGAATG AAATCCTGAA CGGCATGGAT GAAGTGCTGG ATAGCCCGGA AGACCTCGAA 
AAGAAGCGGA TCTGCCGCAT CATCACCCGT GACTTCCCAC AGTACTTTGC GGTGGTGTCG 
CGCATCAAGC AGGACAGCAA CCTGATTGGC CCGGAGGGAG GCGTGCTGAG CAGCACCGTG 
GTGCCGCAGG TGCAGGCCGT GTTCCCCGAG GGGGCGCTAA CCAAGCGGAT CCGTGTTGGC 
CTACAGGCTC AACCTATGCA CAGTGAGTTG GTTAAGAAGA TTCTGGGCAA CAAAGCTACC 
TTCAGCCCTA TAGTTACTTT GGAACCTAGA AGAAGAAAAT TTCACAAGCC AATTACCATG 
ACCATTCCTG TCCCCAAAGC TTCAAGTGAT GTCATGTTGA ATGGTTTTGG AGGAGATGCA 
CCAACCTTAA GATTACTATG TAGCATCACA GGTGGAACCA CCCCTGCTCA GTGGGAAGAT 
ATTACAGGAA CTACGCCATT AACATTTGTC AATGAATGTG TTTCCTTTAC AACCAATGTG 
TCTGCCAGGT TCTGGCTGAT CGATTGTCGA CAAATTCAGG AATCTGTTAC CTTTGCATCA 
CAAGTGTATA GAGAAATTAT CTGTGTACCA TATATGGCCA AATTTGTAGT GTTTGCCAAA 
TCACATGACC CCATTGAAGC CAGGTTGAGG TGTTTCTGCA TGACTGATGA TAAAGTGGAT 
AAAACCCTGG AACAACAAGA AAACTTTGCT GAGGTGGCCA GAAGCAGGGA TGTGGAGGTC 
TTAGAAGGAA AACCCATCTA TGTTGATTGT TTTGGCAACC TGGTGCCATT AACCAAGAGT 
GGCCAACATC ATATATTCAG TTTTTTTGCC TTCAAAGAAA ACAGACTTCC TCTCTTTGTC 
AAGGTACGTG ATACGACTCA GGAACCTTGC GGACGACTAT CATTTATGAA AGAGCCAAAA 
TCTACAAGAG GCCTGGTGCA CCAAGCTATT TGCAACTTAA ACATCACCCT GCCAATTTAT 
ACGAAGGAAT CAGAGTCAGA TCAAGAACAG GAGGAAGAGA TCGATATGAC ATCAGAAAAA 
AATCAACAGG ATGAGCAGGA ACGGATTGAG GAAAGGCTGG CATATATTGC TGATCACCTT 
GGCTTCAGCT GGACAGAGTT GGCAAGAGAA CTGGACTTCA CTGAAGAGCA AATTCATCAA 
ATTCGAATTG AGAACCCTAA CTCCCTGCAG GATCAGAGTC ATGCGCTGTT GAAGTACTGG 
CTAGAAAGGG ACGGGAGACA TGCTACAGAT ACCAGTCTCG TTGAATGTCT CACCAAGATC 
AACCGAATGG ATATCGTGCA TCTCATGGAG ACCAGCACAG AAGCTCTCCA GGAGCGCATC 
GGTCATAGCT ATGCAGAGAT TGAGCAGACC ATTACACTGG ATCATAGTGA AGGGTTCTCA 
GTACTCCAAG AGGAGCTTTG CACTGCCCAG CACAAGCAGA AAGAGGAGCA TGCTGCTTCT 
AAAGAGGACG AGGCCTCTGA CCACCCTCCC ATCGTCTCAG AGGAAGACAT TTCTGTTGGT 
TACTCCACTC TTCAGGATGG CATCCCCAAA ACTGAGGGTG ATGGCTCTGC AACAGAGCTC 
CTTCCCCAGA CTCAAAAGGA GCAAGTTCAA CAGGATTTCT CAGGGAAAAT GCAAGACCTG 
CCTGAGGAGT CGGCTCTTGA ACAGCAAGAA TACTTCGTGA CAACCCCAGG GACAGAAATG 
TCAGAGACTC AGAAGGCCAC GGGAACATCG GGCTCCCCCA CCAAGACACC TGAGGAAATT 
GTCACCCCTT CCGAGGAGGA GAGGCTGTAT CTCCAAACCC CAACATCGAG TGAGCGGGGA 
GGCTCTCCAG TTGTGCAGGA ACCTGAGGAG CCCCCCGAAC GCAGGGAGGA GAGTTCACCT 
CGGAAAACCA GCCTCGTGAT CGTGGAATCG GCTGAGGACC AGCCTCAGGC CTTCGAGAGG 
CTTGATGAAG AGGCTGCTTT TCAAAAGGGA GATGATATGC CTGACATACC CCCAGAAACA 
GTCACAGAAG AAGAATACAT CGATGAGCAT GGACACACCG TGGTAAAGAA GGTTACTAGG 
AAAATCATTA GGCGGTTCAT CTCCTCTGAC GGCACAGAGA AAGAGGAGAT TACGATGCAG 
GGAGCACCAC AGGATTCTAT CAGCGTTGAT GAAGGGGATG GTTATTCCAA AGTCATAAAG 
CGCATTGTAT TGAAGAGTGA CACCGAGCAG TCAGAGGTAA CTTTGTCTGA GCCCAGCATT 
CTGTCCAGTA CCTCACAATT TCAGGCTGAA CCAGTGGAAG GCCGTAGAGT CAGCAAAGTT 
GTTAAGACAA CTGTGGTCCA TGGTGAGAGG ATGGAGAAAC ATCTGGGGGA TTCTAGTTTA 
GCCACTGATC TTCCTTCAGC CAAAGATGAC TTTGAAGAGG ACGACAATGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849265.1
CDS	207..5780
Translation	MTTMLQKSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSAVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQIASTLLSYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMTEVLDVSDEEGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSHTLSHASYLRDSAMIDDAVVIPSHQVTTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGRTSPCLDRDNSSFLVSFMVDARGGAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHYCEYTEEELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEIDMTSEKNQQDEQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGRHATDTSLVECLTKINRMDIVHLMETSTEALQERIGHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEHAASKEDEASDHPPIVSEEDISVGYSTLQDGIPKTEGDGSATELLPQTQKEQVQQDFSGKMQDLPEESALEQQEYFVTTPGTEMSETQKATGTSGSPTKTPEEIVTPSEEERLYLQTPTSSERGGSPVVQEPEEPPERREESSPRKTSLVIVESAEDQPQAFERLDEEAAFQKGDDMPDIPPETVTEEEYIDEHGHTVVKKVTRKIIRRFISSDGTEKEEITMQGAPQDSISVDEGDGYSKVIKRIVLKSDTEQSEVTLSEPSILSSTSQFQAEPVEGRRVSKVVKTTVVHGERMEKHLGDSSLATDLPSAKDDFEEDDNE

Target ORF information:

RefSeq Version	XM_024993497.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin 2 (ANK2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993497.1

ORF Insert Sequence:

ATGACAACCA TGTTGCAAAA GTCTGACAGC AATGCAAGCT TCCTCCGTGC CGCCAGAGCA 
GGCAACTTAG ACAAAGTTGT GGAATATCTG AAGGGGGGCA TAGACATCAA TACCTGCAAC 
CAGAATGGAC TCAACGCGCT TCACCTGGCT GCCAAGGAAG GCCACGTGGG GCTAGTTCAG 
GAGCTGCTGG GAAGAGGGTC TGCTGTGGAC TCTGCCACCA AGAAAGGAAA TACTGCTCTT 
CACATTGCAT CTTTGGCTGG ACAAGCAGAA GTTGTCAAAG TTCTCGTCAA GGAAGGAGCC 
AATATTAATG CACAGTCTCA GAATGGCTTC ACTCCTTTAT ACATGGCTGC CCAAGAGAAT 
CACATTGATG TTGTAAAATA TTTGCTGGAA AATGGAGCTA ATCAGAGCAC CGCTACTGAG 
GATGGCTTTA CGCCCCTGGC GGTGGCGCTC CAGCAGGGAC ACAACCAGGC AGTGGCCATC 
CTCCTGGAGA ACGACACCAA AGGGAAGGTG CGGCTGCCAG CCCTGCATAT TGCAGCCAGG 
AAAGATGACA CCAAATCTGC CGCCCTCCTG CTTCAGAACG ATCACAACGC TGACGTACAG 
TCCAAGATGA TGGTGAATAG GACCACCGAG AGTGGATTCA CCCCTTTGCA CATCGCTGCC 
CACTATGGAA ACGTCAACGT GGCAACTCTC CTTCTAAACC GGGGAGCTGC TGTGGACTTC 
ACAGCCAGGA ATGGAATCAC TCCTCTGCAT GTGGCCTCCA AAAGGGGAAA CACAAACATG 
GTGAAGCTCT TACTGGATCG TGGTGGTCAG ATTGATGCCA AAACTAGGGA TGGGTTGACA 
CCCCTTCATT GTGCTGCAAG AAGCGGTCAT GACCAAGTGG TGGAGCTTCT GCTGGAGCGG 
GGCGCTCCCT TGCTGGCAAG GACGAAGAAC GGGCTGTCTC CACTTCACAT GGCTGCACAG 
GGGGACCACG TGGAATGTGT GAAGCACCTG CTCCAGCACA AGGCGCCTGT TGATGACGTC 
ACTTTGGACT ACTTGACGGC CCTCCACGTT GCCGCACACT GCGGCCACTA CCGAGTAACC 
AAACTTCTTC TGGACAAGAG AGCCAACCCG AACGCCAGAG CCCTGAATGG TTTTACTCCA 
CTGCACATTG CCTGCAAGAA AAACCGCATC AAAGTCATGG AACTGCTGGT GAAATATGGG 
GCTTCAATCC AAGCTATAAC AGAGTCTGGC CTCACACCAA TACATGTGGC TGCCTTCATG 
GGCCACTTGA ACATTGTCCT CCTTCTGCTG CAGAACGGAG CCTCTCCAGA TGTCACTAAC 
ATTCGTGGGG AGACGGCCCT GCACATGGCG GCCCGTGCAG GGCAGGTGGA AGTGGTCCGA 
TGCCTCCTGA GGAACGGTGC CCTCGTCGAT GCCAGAGCCA GGGAGGAACA GACGCCTTTG 
CATATTGCCT CCCGCCTGGG TAAGACAGAA ATTGTTCAGC TGCTTCTGCA ACATATGGCT 
CATCCAGATG CGGCCACTAC AAATGGGTAC ACGCCGTTGC ATATCTCTGC AAGGGAAGGA 
CAGGTGGACG TGGCCTCGGT CCTCCTGGAA GCGGGGGCAG CCCACTCCTT GGCTACCAAG 
AAGGGTTTTA CCCCCTTGCA CGTAGCTGCC AAGTATGGGA GCCTGGATGT GGCAAAACTT 
CTCTTGCAAC GCCGTGCTGC CGCAGATTCT GCGGGGAAGA ATGGCTACAC TCCTTTACAT 
ATTGCTGCCA AGAAGAATCA AATGCAGATA GCTTCCACAC TCCTGAGCTA CGGAGCAGAG 
ACGAACATCG TGACAAAGCA AGGAGTCACT CCACTCCATC TGGCCTCGCA GGAGGGACAC 
ACGGATATGG TCACCTTGCT TCTGGATAAG GGAGCCAATA TCCACATGTC AACCAAGAGT 
GGACTCACAT CTTTACACCT TGCAGCCCAG GAAGATAAAG TGAATGTCGC TGATATTCTC 
ACCAAGCATG GGGCTGACCA GGATGCGCAC ACAAAGCTTG GTTACACTCC TTTAATTGTG 
GCCTGTCACT ATGGAAACGT GAAAATGGTC AACTTCCTTC TGAAGCAGGG AGCAAATGTC 
AATGCAAAAA CCAAGAATGG CTACACGCCT TTGCACCAAG CCGCCCAACA GGGCCACACG 
CACATCATCA ATGTTTTGCT GCAGCACGGG GCCAAGCCCA ACGCTACCAC CGCGAATGGC 
AACACTGCCT TGGCGATTGC TAAGCGTCTG GGCTACATCT CGGTGGTGGA CACCCTGAAG 
GTTGTAACGG AGGAGGTCAC CACCACCACC ACGACTATCA CTGAAAAACA CAAGCTAAAT 
GTTCCTGAGA CCATGACTGA GGTTCTTGAT GTTTCTGATG AAGAGGGTGA TGACACAATG 
ACGGGTGATG GGGGAGAGTA CCTTAGACCT GAGGACCTAA AGGAGCTGGG TGATGACTCC 
CTCCCCAGCA GTCAGTTCCT GGACGGGATG AATTACCTGC GCTACAGCTT GGAGGGGGGC 
CGCTCCGACA GCCTTCGATC CTTCAGTTCC GACAGGTCTC ACACCCTGAG CCATGCCTCG 
TACCTGAGGG ACAGTGCCAT GATCGACGAC GCAGTTGTGA TCCCCAGTCA CCAGGTGACA 
ACTCTAGCCA AGGAGGCAGA AAGGAATTCT TATCGTCTAA GCTGGGGCAC TGAGAACTTG 
GACAACGTGG CTCTTTCTTC CAGCCCTATT CATTCAGGCC GCACCTCTCC ATGTCTTGAT 
CGTGACAACA GCAGTTTCCT GGTTAGTTTT ATGGTGGATG CCCGAGGTGG TGCTATGCGA 
GGATGCAGAC ACAACGGGCT CCGCATCATT ATTCCGCCCC GGAAATGTAC CGCCCCAACA 
CGAGTCACCT GCCGACTGGT CAAACGCCAT AGACTGGCGA CAATGCCTCC AATGGTGGAA 
GGAGAGGGCC TGGCCAGTCG CCTGATTGAA GTTGGACCTT CTGGTGCTCA GTTCCTTGGG 
CCTGTGATCG TGGAGATCCC TCATTTCGCT GCCCTGCGAG GAAAAGAGAG GGAACTGGTG 
GTCCTGCGCA GTGAGAACGG GGACAGCTGG AAAGAGCATT ACTGTGAATA CACGGAGGAG 
GAGCTGAATG AAATCCTGAA CGGCATGGAT GAAGTGCTGG ATAGCCCGGA AGACCTCGAA 
AAGAAGCGGA TCTGCCGCAT CATCACCCGT GACTTCCCAC AGTACTTTGC GGTGGTGTCG 
CGCATCAAGC AGGACAGCAA CCTGATTGGC CCGGAGGGAG GCGTGCTGAG CAGCACCGTG 
GTGCCGCAGG TGCAGGCCGT GTTCCCCGAG GGGGCGCTAA CCAAGCGGAT CCGTGTTGGC 
CTACAGGCTC AACCTATGCA CAGTGAGTTG GTTAAGAAGA TTCTGGGCAA CAAAGCTACC 
TTCAGCCCTA TAGTTACTTT GGAACCTAGA AGAAGAAAAT TTCACAAGCC AATTACCATG 
ACCATTCCTG TCCCCAAAGC TTCAAGTGAT GTCATGTTGA ATGGTTTTGG AGGAGATGCA 
CCAACCTTAA GATTACTATG TAGCATCACA GGTGGAACCA CCCCTGCTCA GTGGGAAGAT 
ATTACAGGAA CTACGCCATT AACATTTGTC AATGAATGTG TTTCCTTTAC AACCAATGTG 
TCTGCCAGGT TCTGGCTGAT CGATTGTCGA CAAATTCAGG AATCTGTTAC CTTTGCATCA 
CAAGTGTATA GAGAAATTAT CTGTGTACCA TATATGGCCA AATTTGTAGT GTTTGCCAAA 
TCACATGACC CCATTGAAGC CAGGTTGAGG TGTTTCTGCA TGACTGATGA TAAAGTGGAT 
AAAACCCTGG AACAACAAGA AAACTTTGCT GAGGTGGCCA GAAGCAGGGA TGTGGAGGTC 
TTAGAAGGAA AACCCATCTA TGTTGATTGT TTTGGCAACC TGGTGCCATT AACCAAGAGT 
GGCCAACATC ATATATTCAG TTTTTTTGCC TTCAAAGAAA ACAGACTTCC TCTCTTTGTC 
AAGGTACGTG ATACGACTCA GGAACCTTGC GGACGACTAT CATTTATGAA AGAGCCAAAA 
TCTACAAGAG GCCTGGTGCA CCAAGCTATT TGCAACTTAA ACATCACCCT GCCAATTTAT 
ACGAAGGAAT CAGAGTCAGA TCAAGAACAG GAGGAAGAGA TCGATATGAC ATCAGAAAAA 
AATCAACAGG ATGAGCAGGA ACGGATTGAG GAAAGGCTGG CATATATTGC TGATCACCTT 
GGCTTCAGCT GGACAGAGTT GGCAAGAGAA CTGGACTTCA CTGAAGAGCA AATTCATCAA 
ATTCGAATTG AGAACCCTAA CTCCCTGCAG GATCAGAGTC ATGCGCTGTT GAAGTACTGG 
CTAGAAAGGG ACGGGAGACA TGCTACAGAT ACCAGTCTCG TTGAATGTCT CACCAAGATC 
AACCGAATGG ATATCGTGCA TCTCATGGAG ACCAGCACAG AAGCTCTCCA GGAGCGCATC 
GGTCATAGCT ATGCAGAGAT TGAGCAGACC ATTACACTGG ATCATAGTGA AGGGTTCTCA 
GTACTCCAAG AGGAGCTTTG CACTGCCCAG CACAAGCAGA AAGAGGAGCA TGCTGCTTCT 
AAAGAGGACG AGGCCTCTGA CCACCCTCCC ATCGTCTCAG AGGAAGACAT TTCTGTTGGT 
TACTCCACTC TTCAGGATGG CATCCCCAAA ACTGAGGGTG ATGGCTCTGC AACAGAGCTC 
CTTCCCCAGA CTCAAAAGGA GCAAGTTCAA CAGGATTTCT CAGGGAAAAT GCAAGACCTG 
CCTGAGGAGT CGGCTCTTGA ACAGCAAGAA TACTTCGTGA CAACCCCAGG GACAGAAATG 
TCAGAGACTC AGAAGGCCAC GGGAACATCG GGCTCCCCCA CCAAGACACC TGAGGAAATT 
GTCACCCCTT CCGAGGAGGA GAGGCTGTAT CTCCAAACCC CAACATCGAG TGAGCGGGGA 
GGCTCTCCAG TTGTGCAGGA ACCTGAGGAG CCCCCCGAAC GCAGGGAGGA GAGTTCACCT 
CGGAAAACCA GCCTCGTGAT CGTGGAATCG GCTGAGGACC AGCCTCAGGC CTTCGAGAGG 
CTTGATGAAG AGGCTGCTTT TCAAAAGGGA GATGATATGC CTGACATACC CCCAGAAACA 
GTCACAGAAG AAGAATACAT CGATGAGCAT GGACACACCG TGGTAAAGAA GGTTACTAGG 
AAAATCATTA GGCGGTTCAT CTCCTCTGAC GGCACAGAGA AAGAGGAGAT TACGATGCAG 
GGAGCACCAC AGGATTCTAT CAGCGTTGAT GAAGGGGATG GTTATTCCAA AGTCATAAAG 
CGCATTGTAT TGAAGAGTGA CACCGAGCAG TCAGAGGTAA CTTTGTCTGA GCCCAGCATT 
CTGTCCAGTA CCTCACAATT TCAGGCTGAA CCAGTGGAAG GCCGTAGAGT CAGCAAAGTT 
GTTAAGACAA CTGTGGTCCA TGGTGAGAGG ATGGAGAAAC ATCTGGGGGA TTCTAGTTTA 
GCCACTGATC TTCCTTCAGC CAAAGATGAC TTTGAAGAGG ACGACAATGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK2 ( XM_024993498.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849266.1 Bos taurus ankyrin 2 (ANK2), transcript variant X14, mRNA.

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CloneID	OBa235490
Clone ID Related Accession (Same CDS sequence)	XM_024993498.1
Accession Version	XM_024993498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin-2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACCA TGTTGCAAAA GTCTGACAGC AATGCAAGCT TCCTCCGTGC CGCCAGAGCA 
GGCAACTTAG ACAAAGTTGT GGAATATCTG AAGGGGGGCA TAGACATCAA TACCTGCAAC 
CAGAATGGAC TCAACGCGCT TCACCTGGCT GCCAAGGAAG GCCACGTGGG GCTAGTTCAG 
GAGCTGCTGG GAAGAGGGTC TGCTGTGGAC TCTGCCACCA AGAAAGGAAA TACTGCTCTT 
CACATTGCAT CTTTGGCTGG ACAAGCAGAA GTTGTCAAAG TTCTCGTCAA GGAAGGAGCC 
AATATTAATG CACAGTCTCA GAATGGCTTC ACTCCTTTAT ACATGGCTGC CCAAGAGAAT 
CACATTGATG TTGTAAAATA TTTGCTGGAA AATGGAGCTA ATCAGAGCAC CGCTACTGAG 
GATGGCTTTA CGCCCCTGGC GGTGGCGCTC CAGCAGGGAC ACAACCAGGC AGTGGCCATC 
CTCCTGGAGA ACGACACCAA AGGGAAGGTG CGGCTGCCAG CCCTGCATAT TGCAGCCAGG 
AAAGATGACA CCAAATCTGC CGCCCTCCTG CTTCAGAACG ATCACAACGC TGACGTACAG 
TCCAAGAGTG GATTCACCCC TTTGCACATC GCTGCCCACT ATGGAAACGT CAACGTGGCA 
ACTCTCCTTC TAAACCGGGG AGCTGCTGTG GACTTCACAG CCAGGAATGG AATCACTCCT 
CTGCATGTGG CCTCCAAAAG GGGAAACACA AACATGGTGA AGCTCTTACT GGATCGTGGT 
GGTCAGATTG ATGCCAAAAC TAGGGATGGG TTGACACCCC TTCATTGTGC TGCAAGAAGC 
GGTCATGACC AAGTGGTGGA GCTTCTGCTG GAGCGGGGCG CTCCCTTGCT GGCAAGGACG 
AAGAACGGGC TGTCTCCACT TCACATGGCT GCACAGGGGG ACCACGTGGA ATGTGTGAAG 
CACCTGCTCC AGCACAAGGC GCCTGTTGAT GACGTCACTT TGGACTACTT GACGGCCCTC 
CACGTTGCCG CACACTGCGG CCACTACCGA GTAACCAAAC TTCTTCTGGA CAAGAGAGCC 
AACCCGAACG CCAGAGCCCT GAATGGTTTT ACTCCACTGC ACATTGCCTG CAAGAAAAAC 
CGCATCAAAG TCATGGAACT GCTGGTGAAA TATGGGGCTT CAATCCAAGC TATAACAGAG 
TCTGGCCTCA CACCAATACA TGTGGCTGCC TTCATGGGCC ACTTGAACAT TGTCCTCCTT 
CTGCTGCAGA ACGGAGCCTC TCCAGATGTC ACTAACATTC GTGGGGAGAC GGCCCTGCAC 
ATGGCGGCCC GTGCAGGGCA GGTGGAAGTG GTCCGATGCC TCCTGAGGAA CGGTGCCCTC 
GTCGATGCCA GAGCCAGGGA GGAACAGACG CCTTTGCATA TTGCCTCCCG CCTGGGTAAG 
ACAGAAATTG TTCAGCTGCT TCTGCAACAT ATGGCTCATC CAGATGCGGC CACTACAAAT 
GGGTACACGC CGTTGCATAT CTCTGCAAGG GAAGGACAGG TGGACGTGGC CTCGGTCCTC 
CTGGAAGCGG GGGCAGCCCA CTCCTTGGCT ACCAAGAAGG GTTTTACCCC CTTGCACGTA 
GCTGCCAAGT ATGGGAGCCT GGATGTGGCA AAACTTCTCT TGCAACGCCG TGCTGCCGCA 
GATTCTGCGG GGAAGAATGG CTACACTCCT TTACATATTG CTGCCAAGAA GAATCAAATG 
CAGATAGCTT CCACACTCCT GAGCTACGGA GCAGAGACGA ACATCGTGAC AAAGCAAGGA 
GTCACTCCAC TCCATCTGGC CTCGCAGGAG GGACACACGG ATATGGTCAC CTTGCTTCTG 
GATAAGGGAG CCAATATCCA CATGTCAACC AAGAGTGGAC TCACATCTTT ACACCTTGCA 
GCCCAGGAAG ATAAAGTGAA TGTCGCTGAT ATTCTCACCA AGCATGGGGC TGACCAGGAT 
GCGCACACAA AGCTTGGTTA CACTCCTTTA ATTGTGGCCT GTCACTATGG AAACGTGAAA 
ATGGTCAACT TCCTTCTGAA GCAGGGAGCA AATGTCAATG CAAAAACCAA GAATGGCTAC 
ACGCCTTTGC ACCAAGCCGC CCAACAGGGC CACACGCACA TCATCAATGT TTTGCTGCAG 
CACGGGGCCA AGCCCAACGC TACCACCGCG AATGGCAACA CTGCCTTGGC GATTGCTAAG 
CGTCTGGGCT ACATCTCGGT GGTGGACACC CTGAAGGTTG TAACGGAGGA GGTCACCACC 
ACCACCACGA CTATCACTGA AAAACACAAG CTAAATGTTC CTGAGACCAT GACTGAGGTT 
CTTGATGTTT CTGATGAAGA GGGTGATGAC ACAATGACGG GTGATGGGGG AGAGTACCTT 
AGACCTGAGG ACCTAAAGGA GCTGGGTGAT GACTCCCTCC CCAGCAGTCA GTTCCTGGAC 
GGGATGAATT ACCTGCGCTA CAGCTTGGAG GGGGGCCGCT CCGACAGCCT TCGATCCTTC 
AGTTCCGACA GGTCTCACAC CCTGAGCCAT GCCTCGTACC TGAGGGACAG TGCCATGATC 
GACGACGCAG TTGTGATCCC CAGTCACCAG GTGACAACTC TAGCCAAGGA GGCAGAAAGG 
AATTCTTATC GTCTAAGCTG GGGCACTGAG AACTTGGACA ACGTGGCTCT TTCTTCCAGC 
CCTATTCATT CAGGCCGCAC CTCTCCATGT CTTGATCGTG ACAACAGCAG TTTCCTGGTT 
AGTTTTATGG TGGATGCCCG AGGTGGTGCT ATGCGAGGAT GCAGACACAA CGGGCTCCGC 
ATCATTATTC CGCCCCGGAA ATGTACCGCC CCAACACGAG TCACCTGCCG ACTGGTCAAA 
CGCCATAGAC TGGCGACAAT GCCTCCAATG GTGGAAGGAG AGGGCCTGGC CAGTCGCCTG 
ATTGAAGTTG GACCTTCTGG TGCTCAGTTC CTTGGGCCTG TGATCGTGGA GATCCCTCAT 
TTCGCTGCCC TGCGAGGAAA AGAGAGGGAA CTGGTGGTCC TGCGCAGTGA GAACGGGGAC 
AGCTGGAAAG AGCATTACTG TGAATACACG GAGGAGGAGC TGAATGAAAT CCTGAACGGC 
ATGGATGAAG TGCTGGATAG CCCGGAAGAC CTCGAAAAGA AGCGGATCTG CCGCATCATC 
ACCCGTGACT TCCCACAGTA CTTTGCGGTG GTGTCGCGCA TCAAGCAGGA CAGCAACCTG 
ATTGGCCCGG AGGGAGGCGT GCTGAGCAGC ACCGTGGTGC CGCAGGTGCA GGCCGTGTTC 
CCCGAGGGGG CGCTAACCAA GCGGATCCGT GTTGGCCTAC AGGCTCAACC TATGCACAGT 
GAGTTGGTTA AGAAGATTCT GGGCAACAAA GCTACCTTCA GCCCTATAGT TACTTTGGAA 
CCTAGAAGAA GAAAATTTCA CAAGCCAATT ACCATGACCA TTCCTGTCCC CAAAGCTTCA 
AGTGATGTCA TGTTGAATGG TTTTGGAGGA GATGCACCAA CCTTAAGATT ACTATGTAGC 
ATCACAGGTG GAACCACCCC TGCTCAGTGG GAAGATATTA CAGGAACTAC GCCATTAACA 
TTTGTCAATG AATGTGTTTC CTTTACAACC AATGTGTCTG CCAGGTTCTG GCTGATCGAT 
TGTCGACAAA TTCAGGAATC TGTTACCTTT GCATCACAAG TGTATAGAGA AATTATCTGT 
GTACCATATA TGGCCAAATT TGTAGTGTTT GCCAAATCAC ATGACCCCAT TGAAGCCAGG 
TTGAGGTGTT TCTGCATGAC TGATGATAAA GTGGATAAAA CCCTGGAACA ACAAGAAAAC 
TTTGCTGAGG TGGCCAGAAG CAGGGATGTG GAGGTCTTAG AAGGAAAACC CATCTATGTT 
GATTGTTTTG GCAACCTGGT GCCATTAACC AAGAGTGGCC AACATCATAT ATTCAGTTTT 
TTTGCCTTCA AAGAAAACAG ACTTCCTCTC TTTGTCAAGG TACGTGATAC GACTCAGGAA 
CCTTGCGGAC GACTATCATT TATGAAAGAG CCAAAATCTA CAAGAGGCCT GGTGCACCAA 
GCTATTTGCA ACTTAAACAT CACCCTGCCA ATTTATACGA AGGAATCAGA GTCAGATCAA 
GAACAGGAGG AAGAGATCGA TATGACATCA GAAAAAAATC AACAGGATGA GCAGGAACGG 
ATTGAGGAAA GGCTGGCATA TATTGCTGAT CACCTTGGCT TCAGCTGGAC AGAGTTGGCA 
AGAGAACTGG ACTTCACTGA AGAGCAAATT CATCAAATTC GAATTGAGAA CCCTAACTCC 
CTGCAGGATC AGAGTCATGC GCTGTTGAAG TACTGGCTAG AAAGGGACGG GAGACATGCT 
ACAGATACCA GTCTCGTTGA ATGTCTCACC AAGATCAACC GAATGGATAT CGTGCATCTC 
ATGGAGACCA GCACAGAAGC TCTCCAGGAG CGCATCGGTC ATAGCTATGC AGAGATTGAG 
CAGACCATTA CACTGGATCA TAGTGAAGGG TTCTCAGTAC TCCAAGAGGA GCTTTGCACT 
GCCCAGCACA AGCAGAAAGA GGAGCATGCT GCTTCTAAAG AGGACGAGGC CTCTGACCAC 
CCTCCCATCG TCTCAGAGGA AGACATTTCT GTTGGTTACT CCACTCTTCA GGATGGCATC 
CCCAAAACTG AGGGTGATGG CTCTGCAACA GAGCTCCTTC CCCAGACTCA AAAGGAGCAA 
GTTCAACAGG ATTTCTCAGG GAAAATGCAA GACCTGCCTG AGGAGTCGGC TCTTGAACAG 
CAAGAATACT TCGTGACAAC CCCAGGGACA GAAATGTCAG AGACTCAGAA GGCCACGGGA 
ACATCGGGCT CCCCCACCAA GACACCTGAG GAAATTGTCA CCCCTTCCGA GGAGGAGAGG 
CTGTATCTCC AAACCCCAAC ATCGAGTGAG CGGGGAGGCT CTCCAGTTGT GCAGGAACCT 
GAGGAGCCCC CCGAACGCAG GGAGGAGAGT TCACCTCGGA AAACCAGCCT CGTGATCGTG 
GAATCGGCTG AGGACCAGCC TCAGGCCTTC GAGAGGCTTG ATGAAGAGGC TGCTTTTCAA 
AAGGGAGATG ATATGCCTGA CATACCCCCA GAAACAGTCA CAGAAGAAGA ATACATCGAT 
GAGCATGGAC ACACCGTGGT AAAGAAGGTT ACTAGGAAAA TCATTAGGCG GTTCATCTCC 
TCTGACGGCA CAGAGAAAGA GGAGATTACG ATGCAGGGAG CACCACAGGA TTCTATCAGC 
GTTGATGAAG GGGATGGTTA TTCCAAAGTC ATAAAGCGCA TTGTATTGAA GAGTGACACC 
GAGCAGTCAG AGGTAACTTT GTCTGAGCCC AGCATTCTGT CCAGTACCTC ACAATTTCAG 
GCTGAACCAG TGGAAGGCCG TAGAGTCAGC AAAGTTGTTA AGACAACTGT GGTCCATGGT 
GAGAGGATGG AGAAACATCT GGGGGATTCT AGTTTAGCCA CTGATCTTCC TTCAGCCAAA 
GATGACTTTG AAGAGGACGA CAATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849266.1
CDS	207..5756
Translation	MTTMLQKSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSAVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQIASTLLSYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMTEVLDVSDEEGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSHTLSHASYLRDSAMIDDAVVIPSHQVTTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGRTSPCLDRDNSSFLVSFMVDARGGAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHYCEYTEEELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEIDMTSEKNQQDEQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGRHATDTSLVECLTKINRMDIVHLMETSTEALQERIGHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEHAASKEDEASDHPPIVSEEDISVGYSTLQDGIPKTEGDGSATELLPQTQKEQVQQDFSGKMQDLPEESALEQQEYFVTTPGTEMSETQKATGTSGSPTKTPEEIVTPSEEERLYLQTPTSSERGGSPVVQEPEEPPERREESSPRKTSLVIVESAEDQPQAFERLDEEAAFQKGDDMPDIPPETVTEEEYIDEHGHTVVKKVTRKIIRRFISSDGTEKEEITMQGAPQDSISVDEGDGYSKVIKRIVLKSDTEQSEVTLSEPSILSSTSQFQAEPVEGRRVSKVVKTTVVHGERMEKHLGDSSLATDLPSAKDDFEEDDNE

Target ORF information:

RefSeq Version	XM_024993498.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin 2 (ANK2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993498.1

ORF Insert Sequence:

ATGACAACCA TGTTGCAAAA GTCTGACAGC AATGCAAGCT TCCTCCGTGC CGCCAGAGCA 
GGCAACTTAG ACAAAGTTGT GGAATATCTG AAGGGGGGCA TAGACATCAA TACCTGCAAC 
CAGAATGGAC TCAACGCGCT TCACCTGGCT GCCAAGGAAG GCCACGTGGG GCTAGTTCAG 
GAGCTGCTGG GAAGAGGGTC TGCTGTGGAC TCTGCCACCA AGAAAGGAAA TACTGCTCTT 
CACATTGCAT CTTTGGCTGG ACAAGCAGAA GTTGTCAAAG TTCTCGTCAA GGAAGGAGCC 
AATATTAATG CACAGTCTCA GAATGGCTTC ACTCCTTTAT ACATGGCTGC CCAAGAGAAT 
CACATTGATG TTGTAAAATA TTTGCTGGAA AATGGAGCTA ATCAGAGCAC CGCTACTGAG 
GATGGCTTTA CGCCCCTGGC GGTGGCGCTC CAGCAGGGAC ACAACCAGGC AGTGGCCATC 
CTCCTGGAGA ACGACACCAA AGGGAAGGTG CGGCTGCCAG CCCTGCATAT TGCAGCCAGG 
AAAGATGACA CCAAATCTGC CGCCCTCCTG CTTCAGAACG ATCACAACGC TGACGTACAG 
TCCAAGAGTG GATTCACCCC TTTGCACATC GCTGCCCACT ATGGAAACGT CAACGTGGCA 
ACTCTCCTTC TAAACCGGGG AGCTGCTGTG GACTTCACAG CCAGGAATGG AATCACTCCT 
CTGCATGTGG CCTCCAAAAG GGGAAACACA AACATGGTGA AGCTCTTACT GGATCGTGGT 
GGTCAGATTG ATGCCAAAAC TAGGGATGGG TTGACACCCC TTCATTGTGC TGCAAGAAGC 
GGTCATGACC AAGTGGTGGA GCTTCTGCTG GAGCGGGGCG CTCCCTTGCT GGCAAGGACG 
AAGAACGGGC TGTCTCCACT TCACATGGCT GCACAGGGGG ACCACGTGGA ATGTGTGAAG 
CACCTGCTCC AGCACAAGGC GCCTGTTGAT GACGTCACTT TGGACTACTT GACGGCCCTC 
CACGTTGCCG CACACTGCGG CCACTACCGA GTAACCAAAC TTCTTCTGGA CAAGAGAGCC 
AACCCGAACG CCAGAGCCCT GAATGGTTTT ACTCCACTGC ACATTGCCTG CAAGAAAAAC 
CGCATCAAAG TCATGGAACT GCTGGTGAAA TATGGGGCTT CAATCCAAGC TATAACAGAG 
TCTGGCCTCA CACCAATACA TGTGGCTGCC TTCATGGGCC ACTTGAACAT TGTCCTCCTT 
CTGCTGCAGA ACGGAGCCTC TCCAGATGTC ACTAACATTC GTGGGGAGAC GGCCCTGCAC 
ATGGCGGCCC GTGCAGGGCA GGTGGAAGTG GTCCGATGCC TCCTGAGGAA CGGTGCCCTC 
GTCGATGCCA GAGCCAGGGA GGAACAGACG CCTTTGCATA TTGCCTCCCG CCTGGGTAAG 
ACAGAAATTG TTCAGCTGCT TCTGCAACAT ATGGCTCATC CAGATGCGGC CACTACAAAT 
GGGTACACGC CGTTGCATAT CTCTGCAAGG GAAGGACAGG TGGACGTGGC CTCGGTCCTC 
CTGGAAGCGG GGGCAGCCCA CTCCTTGGCT ACCAAGAAGG GTTTTACCCC CTTGCACGTA 
GCTGCCAAGT ATGGGAGCCT GGATGTGGCA AAACTTCTCT TGCAACGCCG TGCTGCCGCA 
GATTCTGCGG GGAAGAATGG CTACACTCCT TTACATATTG CTGCCAAGAA GAATCAAATG 
CAGATAGCTT CCACACTCCT GAGCTACGGA GCAGAGACGA ACATCGTGAC AAAGCAAGGA 
GTCACTCCAC TCCATCTGGC CTCGCAGGAG GGACACACGG ATATGGTCAC CTTGCTTCTG 
GATAAGGGAG CCAATATCCA CATGTCAACC AAGAGTGGAC TCACATCTTT ACACCTTGCA 
GCCCAGGAAG ATAAAGTGAA TGTCGCTGAT ATTCTCACCA AGCATGGGGC TGACCAGGAT 
GCGCACACAA AGCTTGGTTA CACTCCTTTA ATTGTGGCCT GTCACTATGG AAACGTGAAA 
ATGGTCAACT TCCTTCTGAA GCAGGGAGCA AATGTCAATG CAAAAACCAA GAATGGCTAC 
ACGCCTTTGC ACCAAGCCGC CCAACAGGGC CACACGCACA TCATCAATGT TTTGCTGCAG 
CACGGGGCCA AGCCCAACGC TACCACCGCG AATGGCAACA CTGCCTTGGC GATTGCTAAG 
CGTCTGGGCT ACATCTCGGT GGTGGACACC CTGAAGGTTG TAACGGAGGA GGTCACCACC 
ACCACCACGA CTATCACTGA AAAACACAAG CTAAATGTTC CTGAGACCAT GACTGAGGTT 
CTTGATGTTT CTGATGAAGA GGGTGATGAC ACAATGACGG GTGATGGGGG AGAGTACCTT 
AGACCTGAGG ACCTAAAGGA GCTGGGTGAT GACTCCCTCC CCAGCAGTCA GTTCCTGGAC 
GGGATGAATT ACCTGCGCTA CAGCTTGGAG GGGGGCCGCT CCGACAGCCT TCGATCCTTC 
AGTTCCGACA GGTCTCACAC CCTGAGCCAT GCCTCGTACC TGAGGGACAG TGCCATGATC 
GACGACGCAG TTGTGATCCC CAGTCACCAG GTGACAACTC TAGCCAAGGA GGCAGAAAGG 
AATTCTTATC GTCTAAGCTG GGGCACTGAG AACTTGGACA ACGTGGCTCT TTCTTCCAGC 
CCTATTCATT CAGGCCGCAC CTCTCCATGT CTTGATCGTG ACAACAGCAG TTTCCTGGTT 
AGTTTTATGG TGGATGCCCG AGGTGGTGCT ATGCGAGGAT GCAGACACAA CGGGCTCCGC 
ATCATTATTC CGCCCCGGAA ATGTACCGCC CCAACACGAG TCACCTGCCG ACTGGTCAAA 
CGCCATAGAC TGGCGACAAT GCCTCCAATG GTGGAAGGAG AGGGCCTGGC CAGTCGCCTG 
ATTGAAGTTG GACCTTCTGG TGCTCAGTTC CTTGGGCCTG TGATCGTGGA GATCCCTCAT 
TTCGCTGCCC TGCGAGGAAA AGAGAGGGAA CTGGTGGTCC TGCGCAGTGA GAACGGGGAC 
AGCTGGAAAG AGCATTACTG TGAATACACG GAGGAGGAGC TGAATGAAAT CCTGAACGGC 
ATGGATGAAG TGCTGGATAG CCCGGAAGAC CTCGAAAAGA AGCGGATCTG CCGCATCATC 
ACCCGTGACT TCCCACAGTA CTTTGCGGTG GTGTCGCGCA TCAAGCAGGA CAGCAACCTG 
ATTGGCCCGG AGGGAGGCGT GCTGAGCAGC ACCGTGGTGC CGCAGGTGCA GGCCGTGTTC 
CCCGAGGGGG CGCTAACCAA GCGGATCCGT GTTGGCCTAC AGGCTCAACC TATGCACAGT 
GAGTTGGTTA AGAAGATTCT GGGCAACAAA GCTACCTTCA GCCCTATAGT TACTTTGGAA 
CCTAGAAGAA GAAAATTTCA CAAGCCAATT ACCATGACCA TTCCTGTCCC CAAAGCTTCA 
AGTGATGTCA TGTTGAATGG TTTTGGAGGA GATGCACCAA CCTTAAGATT ACTATGTAGC 
ATCACAGGTG GAACCACCCC TGCTCAGTGG GAAGATATTA CAGGAACTAC GCCATTAACA 
TTTGTCAATG AATGTGTTTC CTTTACAACC AATGTGTCTG CCAGGTTCTG GCTGATCGAT 
TGTCGACAAA TTCAGGAATC TGTTACCTTT GCATCACAAG TGTATAGAGA AATTATCTGT 
GTACCATATA TGGCCAAATT TGTAGTGTTT GCCAAATCAC ATGACCCCAT TGAAGCCAGG 
TTGAGGTGTT TCTGCATGAC TGATGATAAA GTGGATAAAA CCCTGGAACA ACAAGAAAAC 
TTTGCTGAGG TGGCCAGAAG CAGGGATGTG GAGGTCTTAG AAGGAAAACC CATCTATGTT 
GATTGTTTTG GCAACCTGGT GCCATTAACC AAGAGTGGCC AACATCATAT ATTCAGTTTT 
TTTGCCTTCA AAGAAAACAG ACTTCCTCTC TTTGTCAAGG TACGTGATAC GACTCAGGAA 
CCTTGCGGAC GACTATCATT TATGAAAGAG CCAAAATCTA CAAGAGGCCT GGTGCACCAA 
GCTATTTGCA ACTTAAACAT CACCCTGCCA ATTTATACGA AGGAATCAGA GTCAGATCAA 
GAACAGGAGG AAGAGATCGA TATGACATCA GAAAAAAATC AACAGGATGA GCAGGAACGG 
ATTGAGGAAA GGCTGGCATA TATTGCTGAT CACCTTGGCT TCAGCTGGAC AGAGTTGGCA 
AGAGAACTGG ACTTCACTGA AGAGCAAATT CATCAAATTC GAATTGAGAA CCCTAACTCC 
CTGCAGGATC AGAGTCATGC GCTGTTGAAG TACTGGCTAG AAAGGGACGG GAGACATGCT 
ACAGATACCA GTCTCGTTGA ATGTCTCACC AAGATCAACC GAATGGATAT CGTGCATCTC 
ATGGAGACCA GCACAGAAGC TCTCCAGGAG CGCATCGGTC ATAGCTATGC AGAGATTGAG 
CAGACCATTA CACTGGATCA TAGTGAAGGG TTCTCAGTAC TCCAAGAGGA GCTTTGCACT 
GCCCAGCACA AGCAGAAAGA GGAGCATGCT GCTTCTAAAG AGGACGAGGC CTCTGACCAC 
CCTCCCATCG TCTCAGAGGA AGACATTTCT GTTGGTTACT CCACTCTTCA GGATGGCATC 
CCCAAAACTG AGGGTGATGG CTCTGCAACA GAGCTCCTTC CCCAGACTCA AAAGGAGCAA 
GTTCAACAGG ATTTCTCAGG GAAAATGCAA GACCTGCCTG AGGAGTCGGC TCTTGAACAG 
CAAGAATACT TCGTGACAAC CCCAGGGACA GAAATGTCAG AGACTCAGAA GGCCACGGGA 
ACATCGGGCT CCCCCACCAA GACACCTGAG GAAATTGTCA CCCCTTCCGA GGAGGAGAGG 
CTGTATCTCC AAACCCCAAC ATCGAGTGAG CGGGGAGGCT CTCCAGTTGT GCAGGAACCT 
GAGGAGCCCC CCGAACGCAG GGAGGAGAGT TCACCTCGGA AAACCAGCCT CGTGATCGTG 
GAATCGGCTG AGGACCAGCC TCAGGCCTTC GAGAGGCTTG ATGAAGAGGC TGCTTTTCAA 
AAGGGAGATG ATATGCCTGA CATACCCCCA GAAACAGTCA CAGAAGAAGA ATACATCGAT 
GAGCATGGAC ACACCGTGGT AAAGAAGGTT ACTAGGAAAA TCATTAGGCG GTTCATCTCC 
TCTGACGGCA CAGAGAAAGA GGAGATTACG ATGCAGGGAG CACCACAGGA TTCTATCAGC 
GTTGATGAAG GGGATGGTTA TTCCAAAGTC ATAAAGCGCA TTGTATTGAA GAGTGACACC 
GAGCAGTCAG AGGTAACTTT GTCTGAGCCC AGCATTCTGT CCAGTACCTC ACAATTTCAG 
GCTGAACCAG TGGAAGGCCG TAGAGTCAGC AAAGTTGTTA AGACAACTGT GGTCCATGGT 
GAGAGGATGG AGAAACATCT GGGGGATTCT AGTTTAGCCA CTGATCTTCC TTCAGCCAAA 
GATGACTTTG AAGAGGACGA CAATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK2 ( XM_024993486.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849254.1 Bos taurus ankyrin 2 (ANK2), transcript variant X2, mRNA.

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CloneID	OBa235478
Clone ID Related Accession (Same CDS sequence)	XM_024993486.1
Accession Version	XM_024993486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
ATGATGGTGA ATAGGACCAC CGAGAGTGGA TTCACCCCTT TGCACATCGC TGCCCACTAT 
GGAAACGTCA ACGTGGCAAC TCTCCTTCTA AACCGGGGAG CTGCTGTGGA CTTCACAGCC 
AGGAATGGAA TCACTCCTCT GCATGTGGCC TCCAAAAGGG GAAACACAAA CATGGTGAAG 
CTCTTACTGG ATCGTGGTGG TCAGATTGAT GCCAAAACTA GGGATGGGTT GACACCCCTT 
CATTGTGCTG CAAGAAGCGG TCATGACCAA GTGGTGGAGC TTCTGCTGGA GCGGGGCGCT 
CCCTTGCTGG CAAGGACGAA GAACGGGCTG TCTCCACTTC ACATGGCTGC ACAGGGGGAC 
CACGTGGAAT GTGTGAAGCA CCTGCTCCAG CACAAGGCGC CTGTTGATGA CGTCACTTTG 
GACTACTTGA CGGCCCTCCA CGTTGCCGCA CACTGCGGCC ACTACCGAGT AACCAAACTT 
CTTCTGGACA AGAGAGCCAA CCCGAACGCC AGAGCCCTGA ATGGTTTTAC TCCACTGCAC 
ATTGCCTGCA AGAAAAACCG CATCAAAGTC ATGGAACTGC TGGTGAAATA TGGGGCTTCA 
ATCCAAGCTA TAACAGAGTC TGGCCTCACA CCAATACATG TGGCTGCCTT CATGGGCCAC 
TTGAACATTG TCCTCCTTCT GCTGCAGAAC GGAGCCTCTC CAGATGTCAC TAACATTCGT 
GGGGAGACGG CCCTGCACAT GGCGGCCCGT GCAGGGCAGG TGGAAGTGGT CCGATGCCTC 
CTGAGGAACG GTGCCCTCGT CGATGCCAGA GCCAGGGAGG AACAGACGCC TTTGCATATT 
GCCTCCCGCC TGGGTAAGAC AGAAATTGTT CAGCTGCTTC TGCAACATAT GGCTCATCCA 
GATGCGGCCA CTACAAATGG GTACACGCCG TTGCATATCT CTGCAAGGGA AGGACAGGTG 
GACGTGGCCT CGGTCCTCCT GGAAGCGGGG GCAGCCCACT CCTTGGCTAC CAAGAAGGGT 
TTTACCCCCT TGCACGTAGC TGCCAAGTAT GGGAGCCTGG ATGTGGCAAA ACTTCTCTTG 
CAACGCCGTG CTGCCGCAGA TTCTGCGGGG AAGAATGGCC TTACCCCGCT CCATGTTGCT 
GCTCACTATG ACAACCAGAA GGTGGCGCTG CTGTTACTGG AGAAGGGTGC TTCCCCTCAC 
GCCATGGCCA AGAATGGCTA CACTCCTTTA CATATTGCTG CCAAGAAGAA TCAAATGCAG 
ATAGCTTCCA CACTCCTGAG CTACGGAGCA GAGACGAACA TCGTGACAAA GCAAGGAGTC 
ACTCCACTCC ATCTGGCCTC GCAGGAGGGA CACACGGATA TGGTCACCTT GCTTCTGGAT 
AAGGGAGCCA ATATCCACAT GTCAACCAAG AGTGGACTCA CATCTTTACA CCTTGCAGCC 
CAGGAAGATA AAGTGAATGT CGCTGATATT CTCACCAAGC ATGGGGCTGA CCAGGATGCG 
CACACAAAGC TTGGTTACAC TCCTTTAATT GTGGCCTGTC ACTATGGAAA CGTGAAAATG 
GTCAACTTCC TTCTGAAGCA GGGAGCAAAT GTCAATGCAA AAACCAAGAA TGGCTACACG 
CCTTTGCACC AAGCCGCCCA ACAGGGCCAC ACGCACATCA TCAATGTTTT GCTGCAGCAC 
GGGGCCAAGC CCAACGCTAC CACCGCGAAT GGCAACACTG CCTTGGCGAT TGCTAAGCGT 
CTGGGCTACA TCTCGGTGGT GGACACCCTG AAGGTTGTAA CGGAGGAGGT CACCACCACC 
ACCACGACTA TCACTGAAAA ACACAAGCTA AATGTTCCTG AGACCATGAC TGAGGTTCTT 
GATGTTTCTG ATGAAGAGGG TGATGACACA ATGACGGGTG ATGGGGGAGA GTACCTTAGA 
CCTGAGGACC TAAAGGAGCT GGGTGATGAC TCCCTCCCCA GCAGTCAGTT CCTGGACGGG 
ATGAATTACC TGCGCTACAG CTTGGAGGGG GGCCGCTCCG ACAGCCTTCG ATCCTTCAGT 
TCCGACAGGT CTCACACCCT GAGCCATGCC TCGTACCTGA GGGACAGTGC CATGATCGAC 
GACGCAGTTG TGATCCCCAG TCACCAGGTG ACAACTCTAG CCAAGGAGGC AGAAAGGAAT 
TCTTATCGTC TAAGCTGGGG CACTGAGAAC TTGGACAACG TGGCTCTTTC TTCCAGCCCT 
ATTCATTCAG GCCGCACCTC TCCATGTCTT GATCGTGACA ACAGCAGTTT CCTGGTTAGT 
TTTATGGTGG ATGCCCGAGG TGGTGCTATG CGAGGATGCA GACACAACGG GCTCCGCATC 
ATTATTCCGC CCCGGAAATG TACCGCCCCA ACACGAGTCA CCTGCCGACT GGTCAAACGC 
CATAGACTGG CGACAATGCC TCCAATGGTG GAAGGAGAGG GCCTGGCCAG TCGCCTGATT 
GAAGTTGGAC CTTCTGGTGC TCAGTTCCTT GGGCCTGTGA TCGTGGAGAT CCCTCATTTC 
GCTGCCCTGC GAGGAAAAGA GAGGGAACTG GTGGTCCTGC GCAGTGAGAA CGGGGACAGC 
TGGAAAGAGC ATTACTGTGA ATACACGGAG GAGGAGCTGA ATGAAATCCT GAACGGCATG 
GATGAAGTGC TGGATAGCCC GGAAGACCTC GAAAAGAAGC GGATCTGCCG CATCATCACC 
CGTGACTTCC CACAGTACTT TGCGGTGGTG TCGCGCATCA AGCAGGACAG CAACCTGATT 
GGCCCGGAGG GAGGCGTGCT GAGCAGCACC GTGGTGCCGC AGGTGCAGGC CGTGTTCCCC 
GAGGGGGCGC TAACCAAGCG GATCCGTGTT GGCCTACAGG CTCAACCTAT GCACAGTGAG 
TTGGTTAAGA AGATTCTGGG CAACAAAGCT ACCTTCAGCC CTATAGTTAC TTTGGAACCT 
AGAAGAAGAA AATTTCACAA GCCAATTACC ATGACCATTC CTGTCCCCAA AGCTTCAAGT 
GATGTCATGT TGAATGGTTT TGGAGGAGAT GCACCAACCT TAAGATTACT ATGTAGCATC 
ACAGGTGGAA CCACCCCTGC TCAGTGGGAA GATATTACAG GAACTACGCC ATTAACATTT 
GTCAATGAAT GTGTTTCCTT TACAACCAAT GTGTCTGCCA GGTTCTGGCT GATCGATTGT 
CGACAAATTC AGGAATCTGT TACCTTTGCA TCACAAGTGT ATAGAGAAAT TATCTGTGTA 
CCATATATGG CCAAATTTGT AGTGTTTGCC AAATCACATG ACCCCATTGA AGCCAGGTTG 
AGGTGTTTCT GCATGACTGA TGATAAAGTG GATAAAACCC TGGAACAACA AGAAAACTTT 
GCTGAGGTGG CCAGAAGCAG GGATGTGGAG GTCTTAGAAG GAAAACCCAT CTATGTTGAT 
TGTTTTGGCA ACCTGGTGCC ATTAACCAAG AGTGGCCAAC ATCATATATT CAGTTTTTTT 
GCCTTCAAAG AAAACAGACT TCCTCTCTTT GTCAAGGTAC GTGATACGAC TCAGGAACCT 
TGCGGACGAC TATCATTTAT GAAAGAGCCA AAATCTACAA GAGGCCTGGT GCACCAAGCT 
ATTTGCAACT TAAACATCAC CCTGCCAATT TATACGAAGG AATCAGAGTC AGATCAAGAA 
CAGGAGGAAG AGATCGATAT GACATCAGAA AAAAATCAAC AGGATGAGCA GGAACGGATT 
GAGGAAAGGC TGGCATATAT TGCTGATCAC CTTGGCTTCA GCTGGACAGA GTTGGCAAGA 
GAACTGGACT TCACTGAAGA GCAAATTCAT CAAATTCGAA TTGAGAACCC TAACTCCCTG 
CAGGATCAGA GTCATGCGCT GTTGAAGTAC TGGCTAGAAA GGGACGGGAG ACATGCTACA 
GATACCAGTC TCGTTGAATG TCTCACCAAG ATCAACCGAA TGGATATCGT GCATCTCATG 
GAGACCAGCA CAGAAGCTCT CCAGGAGCGC ATCGGTCATA GCTATGCAGA GATTGAGCAG 
ACCATTACAC TGGATCATAG TGAAGGGTTC TCAGTACTCC AAGAGGAGCT TTGCACTGCC 
CAGCACAAGC AGAAAGAGGA GCATGCTGCT TCTAAAGAGG ACGAGGCCTC TGACCACCCT 
CCCATCGTCT CAGAGGAAGA CATTTCTGTT GGTTACTCCA CTCTTCAGGA TGGCATCCCC 
AAAACTGAGG GTGATGGCTC TGCAACAGAG CTCCTTCCCC AGACTCAAAA GGAGCAAGTT 
CAACAGGATT TCTCAGGGAA AATGCAAGAC CTGCCTGAGG AGTCGGCTCT TGAACAGCAA 
GAATACTTCG TGACAACCCC AGGGACAGAA ATGTCAGAGA CTCAGAAGGC CACGGGAACA 
TCGGGCTCCC CCACCAAGAC ACCTGAGGAA ATTGTCACCC CTTCCGAGGA GGAGAGGCTG 
TATCTCCAAA CCCCAACATC GAGTGAGCGG GGAGGCTCTC CAGTTGTGCA GGAACCTGAG 
GAGCCCCCCG AACGCAGGGA GGAGAGTTCA CCTCGGAAAA CCAGCCTCGT GATCGTGGAA 
TCGGCTGAGG ACCAGCCTCA GGCCTTCGAG AGGCTTGATG AAGAGGCTGC TTTTCAAAAG 
GGAGATGATA TGCCTGACAT ACCCCCAGAA ACAGTCACAG AAGAAGAATA CATCGATGAG 
CATGGACACA CCGTGGTAAA GAAGGTTACT AGGAAAATCA TTAGGCGGTT CATCTCCTCT 
GACGGCACAG AGAAAGAGGA GATTACGATG CAGGGAGCAC CACAGGATTC TATCAGCGTT 
GATGAAGGGG ATGGTTATTC CAAAGTCATA AAGCGCATTG TATTGAAGAG TGACACCGAG 
CAGTCAGAGG TAACTTTGTC TGAGCCCAGC ATTCTGTCCA GTACCTCACA ATTTCAGGCT 
GAACCAGTGG AAGGCCGTAG AGTCAGCAAA GTTGTTAAGA CAACTGTGGT CCATGGTGAG 
AGGATGGAGA AACATCTGGG GGATTCTAGT TTAGCCACTG ATCTTCCTTC AGCCAAAGAT 
GACTTTGAAG AGGCTTTGAG TTACACAGGT AGCCACATGA AAGTCTACTC CCCCAGTTTA 
GTAGAGAATG AAGTCCTGAA GGAGGATGGA TCAATAATTA GAAGGACGAC AATGAGTAAA 
GCTAGAACCC AGAAGAGGGC TGTGGTGAAG GACCAGCAGG GAAAACGCAT TTACTTGGAG 
CACCTGGAGG ATGTCCCAGA AGCGCTAGAC CAGGACGACC TCCAGCACGA CCTCCAGCAA 
CTCCTTCGGC ATTTCTGCAA GGAGGACTCG AAGCAAGAGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849254.1
CDS	239..6286
Translation	MGNAALCPACHADKSVRAQGHLQELDRTPDYYGCNGEDTKELGPRSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSAVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHAMAKNGYTPLHIAAKKNQMQIASTLLSYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMTEVLDVSDEEGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSHTLSHASYLRDSAMIDDAVVIPSHQVTTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGRTSPCLDRDNSSFLVSFMVDARGGAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHYCEYTEEELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEIDMTSEKNQQDEQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGRHATDTSLVECLTKINRMDIVHLMETSTEALQERIGHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEHAASKEDEASDHPPIVSEEDISVGYSTLQDGIPKTEGDGSATELLPQTQKEQVQQDFSGKMQDLPEESALEQQEYFVTTPGTEMSETQKATGTSGSPTKTPEEIVTPSEEERLYLQTPTSSERGGSPVVQEPEEPPERREESSPRKTSLVIVESAEDQPQAFERLDEEAAFQKGDDMPDIPPETVTEEEYIDEHGHTVVKKVTRKIIRRFISSDGTEKEEITMQGAPQDSISVDEGDGYSKVIKRIVLKSDTEQSEVTLSEPSILSSTSQFQAEPVEGRRVSKVVKTTVVHGERMEKHLGDSSLATDLPSAKDDFEEALSYTGSHMKVYSPSLVENEVLKEDGSIIRRTTMSKARTQKRAVVKDQQGKRIYLEHLEDVPEALDQDDLQHDLQQLLRHFCKEDSKQEAQ

Target ORF information:

RefSeq Version	XM_024993486.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin 2 (ANK2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993486.1

ORF Insert Sequence:

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
ATGATGGTGA ATAGGACCAC CGAGAGTGGA TTCACCCCTT TGCACATCGC TGCCCACTAT 
GGAAACGTCA ACGTGGCAAC TCTCCTTCTA AACCGGGGAG CTGCTGTGGA CTTCACAGCC 
AGGAATGGAA TCACTCCTCT GCATGTGGCC TCCAAAAGGG GAAACACAAA CATGGTGAAG 
CTCTTACTGG ATCGTGGTGG TCAGATTGAT GCCAAAACTA GGGATGGGTT GACACCCCTT 
CATTGTGCTG CAAGAAGCGG TCATGACCAA GTGGTGGAGC TTCTGCTGGA GCGGGGCGCT 
CCCTTGCTGG CAAGGACGAA GAACGGGCTG TCTCCACTTC ACATGGCTGC ACAGGGGGAC 
CACGTGGAAT GTGTGAAGCA CCTGCTCCAG CACAAGGCGC CTGTTGATGA CGTCACTTTG 
GACTACTTGA CGGCCCTCCA CGTTGCCGCA CACTGCGGCC ACTACCGAGT AACCAAACTT 
CTTCTGGACA AGAGAGCCAA CCCGAACGCC AGAGCCCTGA ATGGTTTTAC TCCACTGCAC 
ATTGCCTGCA AGAAAAACCG CATCAAAGTC ATGGAACTGC TGGTGAAATA TGGGGCTTCA 
ATCCAAGCTA TAACAGAGTC TGGCCTCACA CCAATACATG TGGCTGCCTT CATGGGCCAC 
TTGAACATTG TCCTCCTTCT GCTGCAGAAC GGAGCCTCTC CAGATGTCAC TAACATTCGT 
GGGGAGACGG CCCTGCACAT GGCGGCCCGT GCAGGGCAGG TGGAAGTGGT CCGATGCCTC 
CTGAGGAACG GTGCCCTCGT CGATGCCAGA GCCAGGGAGG AACAGACGCC TTTGCATATT 
GCCTCCCGCC TGGGTAAGAC AGAAATTGTT CAGCTGCTTC TGCAACATAT GGCTCATCCA 
GATGCGGCCA CTACAAATGG GTACACGCCG TTGCATATCT CTGCAAGGGA AGGACAGGTG 
GACGTGGCCT CGGTCCTCCT GGAAGCGGGG GCAGCCCACT CCTTGGCTAC CAAGAAGGGT 
TTTACCCCCT TGCACGTAGC TGCCAAGTAT GGGAGCCTGG ATGTGGCAAA ACTTCTCTTG 
CAACGCCGTG CTGCCGCAGA TTCTGCGGGG AAGAATGGCC TTACCCCGCT CCATGTTGCT 
GCTCACTATG ACAACCAGAA GGTGGCGCTG CTGTTACTGG AGAAGGGTGC TTCCCCTCAC 
GCCATGGCCA AGAATGGCTA CACTCCTTTA CATATTGCTG CCAAGAAGAA TCAAATGCAG 
ATAGCTTCCA CACTCCTGAG CTACGGAGCA GAGACGAACA TCGTGACAAA GCAAGGAGTC 
ACTCCACTCC ATCTGGCCTC GCAGGAGGGA CACACGGATA TGGTCACCTT GCTTCTGGAT 
AAGGGAGCCA ATATCCACAT GTCAACCAAG AGTGGACTCA CATCTTTACA CCTTGCAGCC 
CAGGAAGATA AAGTGAATGT CGCTGATATT CTCACCAAGC ATGGGGCTGA CCAGGATGCG 
CACACAAAGC TTGGTTACAC TCCTTTAATT GTGGCCTGTC ACTATGGAAA CGTGAAAATG 
GTCAACTTCC TTCTGAAGCA GGGAGCAAAT GTCAATGCAA AAACCAAGAA TGGCTACACG 
CCTTTGCACC AAGCCGCCCA ACAGGGCCAC ACGCACATCA TCAATGTTTT GCTGCAGCAC 
GGGGCCAAGC CCAACGCTAC CACCGCGAAT GGCAACACTG CCTTGGCGAT TGCTAAGCGT 
CTGGGCTACA TCTCGGTGGT GGACACCCTG AAGGTTGTAA CGGAGGAGGT CACCACCACC 
ACCACGACTA TCACTGAAAA ACACAAGCTA AATGTTCCTG AGACCATGAC TGAGGTTCTT 
GATGTTTCTG ATGAAGAGGG TGATGACACA ATGACGGGTG ATGGGGGAGA GTACCTTAGA 
CCTGAGGACC TAAAGGAGCT GGGTGATGAC TCCCTCCCCA GCAGTCAGTT CCTGGACGGG 
ATGAATTACC TGCGCTACAG CTTGGAGGGG GGCCGCTCCG ACAGCCTTCG ATCCTTCAGT 
TCCGACAGGT CTCACACCCT GAGCCATGCC TCGTACCTGA GGGACAGTGC CATGATCGAC 
GACGCAGTTG TGATCCCCAG TCACCAGGTG ACAACTCTAG CCAAGGAGGC AGAAAGGAAT 
TCTTATCGTC TAAGCTGGGG CACTGAGAAC TTGGACAACG TGGCTCTTTC TTCCAGCCCT 
ATTCATTCAG GCCGCACCTC TCCATGTCTT GATCGTGACA ACAGCAGTTT CCTGGTTAGT 
TTTATGGTGG ATGCCCGAGG TGGTGCTATG CGAGGATGCA GACACAACGG GCTCCGCATC 
ATTATTCCGC CCCGGAAATG TACCGCCCCA ACACGAGTCA CCTGCCGACT GGTCAAACGC 
CATAGACTGG CGACAATGCC TCCAATGGTG GAAGGAGAGG GCCTGGCCAG TCGCCTGATT 
GAAGTTGGAC CTTCTGGTGC TCAGTTCCTT GGGCCTGTGA TCGTGGAGAT CCCTCATTTC 
GCTGCCCTGC GAGGAAAAGA GAGGGAACTG GTGGTCCTGC GCAGTGAGAA CGGGGACAGC 
TGGAAAGAGC ATTACTGTGA ATACACGGAG GAGGAGCTGA ATGAAATCCT GAACGGCATG 
GATGAAGTGC TGGATAGCCC GGAAGACCTC GAAAAGAAGC GGATCTGCCG CATCATCACC 
CGTGACTTCC CACAGTACTT TGCGGTGGTG TCGCGCATCA AGCAGGACAG CAACCTGATT 
GGCCCGGAGG GAGGCGTGCT GAGCAGCACC GTGGTGCCGC AGGTGCAGGC CGTGTTCCCC 
GAGGGGGCGC TAACCAAGCG GATCCGTGTT GGCCTACAGG CTCAACCTAT GCACAGTGAG 
TTGGTTAAGA AGATTCTGGG CAACAAAGCT ACCTTCAGCC CTATAGTTAC TTTGGAACCT 
AGAAGAAGAA AATTTCACAA GCCAATTACC ATGACCATTC CTGTCCCCAA AGCTTCAAGT 
GATGTCATGT TGAATGGTTT TGGAGGAGAT GCACCAACCT TAAGATTACT ATGTAGCATC 
ACAGGTGGAA CCACCCCTGC TCAGTGGGAA GATATTACAG GAACTACGCC ATTAACATTT 
GTCAATGAAT GTGTTTCCTT TACAACCAAT GTGTCTGCCA GGTTCTGGCT GATCGATTGT 
CGACAAATTC AGGAATCTGT TACCTTTGCA TCACAAGTGT ATAGAGAAAT TATCTGTGTA 
CCATATATGG CCAAATTTGT AGTGTTTGCC AAATCACATG ACCCCATTGA AGCCAGGTTG 
AGGTGTTTCT GCATGACTGA TGATAAAGTG GATAAAACCC TGGAACAACA AGAAAACTTT 
GCTGAGGTGG CCAGAAGCAG GGATGTGGAG GTCTTAGAAG GAAAACCCAT CTATGTTGAT 
TGTTTTGGCA ACCTGGTGCC ATTAACCAAG AGTGGCCAAC ATCATATATT CAGTTTTTTT 
GCCTTCAAAG AAAACAGACT TCCTCTCTTT GTCAAGGTAC GTGATACGAC TCAGGAACCT 
TGCGGACGAC TATCATTTAT GAAAGAGCCA AAATCTACAA GAGGCCTGGT GCACCAAGCT 
ATTTGCAACT TAAACATCAC CCTGCCAATT TATACGAAGG AATCAGAGTC AGATCAAGAA 
CAGGAGGAAG AGATCGATAT GACATCAGAA AAAAATCAAC AGGATGAGCA GGAACGGATT 
GAGGAAAGGC TGGCATATAT TGCTGATCAC CTTGGCTTCA GCTGGACAGA GTTGGCAAGA 
GAACTGGACT TCACTGAAGA GCAAATTCAT CAAATTCGAA TTGAGAACCC TAACTCCCTG 
CAGGATCAGA GTCATGCGCT GTTGAAGTAC TGGCTAGAAA GGGACGGGAG ACATGCTACA 
GATACCAGTC TCGTTGAATG TCTCACCAAG ATCAACCGAA TGGATATCGT GCATCTCATG 
GAGACCAGCA CAGAAGCTCT CCAGGAGCGC ATCGGTCATA GCTATGCAGA GATTGAGCAG 
ACCATTACAC TGGATCATAG TGAAGGGTTC TCAGTACTCC AAGAGGAGCT TTGCACTGCC 
CAGCACAAGC AGAAAGAGGA GCATGCTGCT TCTAAAGAGG ACGAGGCCTC TGACCACCCT 
CCCATCGTCT CAGAGGAAGA CATTTCTGTT GGTTACTCCA CTCTTCAGGA TGGCATCCCC 
AAAACTGAGG GTGATGGCTC TGCAACAGAG CTCCTTCCCC AGACTCAAAA GGAGCAAGTT 
CAACAGGATT TCTCAGGGAA AATGCAAGAC CTGCCTGAGG AGTCGGCTCT TGAACAGCAA 
GAATACTTCG TGACAACCCC AGGGACAGAA ATGTCAGAGA CTCAGAAGGC CACGGGAACA 
TCGGGCTCCC CCACCAAGAC ACCTGAGGAA ATTGTCACCC CTTCCGAGGA GGAGAGGCTG 
TATCTCCAAA CCCCAACATC GAGTGAGCGG GGAGGCTCTC CAGTTGTGCA GGAACCTGAG 
GAGCCCCCCG AACGCAGGGA GGAGAGTTCA CCTCGGAAAA CCAGCCTCGT GATCGTGGAA 
TCGGCTGAGG ACCAGCCTCA GGCCTTCGAG AGGCTTGATG AAGAGGCTGC TTTTCAAAAG 
GGAGATGATA TGCCTGACAT ACCCCCAGAA ACAGTCACAG AAGAAGAATA CATCGATGAG 
CATGGACACA CCGTGGTAAA GAAGGTTACT AGGAAAATCA TTAGGCGGTT CATCTCCTCT 
GACGGCACAG AGAAAGAGGA GATTACGATG CAGGGAGCAC CACAGGATTC TATCAGCGTT 
GATGAAGGGG ATGGTTATTC CAAAGTCATA AAGCGCATTG TATTGAAGAG TGACACCGAG 
CAGTCAGAGG TAACTTTGTC TGAGCCCAGC ATTCTGTCCA GTACCTCACA ATTTCAGGCT 
GAACCAGTGG AAGGCCGTAG AGTCAGCAAA GTTGTTAAGA CAACTGTGGT CCATGGTGAG 
AGGATGGAGA AACATCTGGG GGATTCTAGT TTAGCCACTG ATCTTCCTTC AGCCAAAGAT 
GACTTTGAAG AGGCTTTGAG TTACACAGGT AGCCACATGA AAGTCTACTC CCCCAGTTTA 
GTAGAGAATG AAGTCCTGAA GGAGGATGGA TCAATAATTA GAAGGACGAC AATGAGTAAA 
GCTAGAACCC AGAAGAGGGC TGTGGTGAAG GACCAGCAGG GAAAACGCAT TTACTTGGAG 
CACCTGGAGG ATGTCCCAGA AGCGCTAGAC CAGGACGACC TCCAGCACGA CCTCCAGCAA 
CTCCTTCGGC ATTTCTGCAA GGAGGACTCG AAGCAAGAGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK2 ( XM_024993487.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849255.1 Bos taurus ankyrin 2 (ANK2), transcript variant X3, mRNA.

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CloneID	OBa235479
Clone ID Related Accession (Same CDS sequence)	XM_024993487.1
Accession Version	XM_024993487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
ATGATGGTGA ATAGGACCAC CGAGAGTGGA TTCACCCCTT TGCACATCGC TGCCCACTAT 
GGAAACGTCA ACGTGGCAAC TCTCCTTCTA AACCGGGGAG CTGCTGTGGA CTTCACAGCC 
AGGAATGGAA TCACTCCTCT GCATGTGGCC TCCAAAAGGG GAAACACAAA CATGGTGAAG 
CTCTTACTGG ATCGTGGTGG TCAGATTGAT GCCAAAACTA GGGATGGGTT GACACCCCTT 
CATTGTGCTG CAAGAAGCGG TCATGACCAA GTGGTGGAGC TTCTGCTGGA GCGGGGCGCT 
CCCTTGCTGG CAAGGACGAA GAACGGGCTG TCTCCACTTC ACATGGCTGC ACAGGGGGAC 
CACGTGGAAT GTGTGAAGCA CCTGCTCCAG CACAAGGCGC CTGTTGATGA CGTCACTTTG 
GACTACTTGA CGGCCCTCCA CGTTGCCGCA CACTGCGGCC ACTACCGAGT AACCAAACTT 
CTTCTGGACA AGAGAGCCAA CCCGAACGCC AGAGCCCTGA ATGGTTTTAC TCCACTGCAC 
ATTGCCTGCA AGAAAAACCG CATCAAAGTC ATGGAACTGC TGGTGAAATA TGGGGCTTCA 
ATCCAAGCTA TAACAGAGTC TGGCCTCACA CCAATACATG TGGCTGCCTT CATGGGCCAC 
TTGAACATTG TCCTCCTTCT GCTGCAGAAC GGAGCCTCTC CAGATGTCAC TAACATTCGT 
GGGGAGACGG CCCTGCACAT GGCGGCCCGT GCAGGGCAGG TGGAAGTGGT CCGATGCCTC 
CTGAGGAACG GTGCCCTCGT CGATGCCAGA GCCAGGGAGG AACAGACGCC TTTGCATATT 
GCCTCCCGCC TGGGTAAGAC AGAAATTGTT CAGCTGCTTC TGCAACATAT GGCTCATCCA 
GATGCGGCCA CTACAAATGG GTACACGCCG TTGCATATCT CTGCAAGGGA AGGACAGGTG 
GACGTGGCCT CGGTCCTCCT GGAAGCGGGG GCAGCCCACT CCTTGGCTAC CAAGAAGGGT 
TTTACCCCCT TGCACGTAGC TGCCAAGTAT GGGAGCCTGG ATGTGGCAAA ACTTCTCTTG 
CAACGCCGTG CTGCCGCAGA TTCTGCGGGG AAGAATGGCC TTACCCCGCT CCATGTTGCT 
GCTCACTATG ACAACCAGAA GGTGGCGCTG CTGTTACTGG AGAAGGGTGC TTCCCCTCAC 
GCCATGGCCA AGAATGGCTA CACTCCTTTA CATATTGCTG CCAAGAAGAA TCAAATGCAG 
ATAGCTTCCA CACTCCTGAG CTACGGAGCA GAGACGAACA TCGTGACAAA GCAAGGAGTC 
ACTCCACTCC ATCTGGCCTC GCAGGAGGGA CACACGGATA TGGTCACCTT GCTTCTGGAT 
AAGGGAGCCA ATATCCACAT GTCAACCAAG AGTGGACTCA CATCTTTACA CCTTGCAGCC 
CAGGAAGATA AAGTGAATGT CGCTGATATT CTCACCAAGC ATGGGGCTGA CCAGGATGCG 
CACACAAAGC TTGGTTACAC TCCTTTAATT GTGGCCTGTC ACTATGGAAA CGTGAAAATG 
GTCAACTTCC TTCTGAAGCA GGGAGCAAAT GTCAATGCAA AAACCAAGAA TGGCTACACG 
CCTTTGCACC AAGCCGCCCA ACAGGGCCAC ACGCACATCA TCAATGTTTT GCTGCAGCAC 
GGGGCCAAGC CCAACGCTAC CACCGCGAAT GGCAACACTG CCTTGGCGAT TGCTAAGCGT 
CTGGGCTACA TCTCGGTGGT GGACACCCTG AAGGTTGTAA CGGAGGAGGT CACCACCACC 
ACCACGACTA TCACTGAAAA ACACAAGCTA AATGTTCCTG AGACCATGAC TGAGGTTCTT 
GATGTTTCTG ATGAAGAGGG TGATGACACA ATGACGGGTG ATGGGGGAGA GTACCTTAGA 
CCTGAGGACC TAAAGGAGCT GGGTGATGAC TCCCTCCCCA GCAGTCAGTT CCTGGACGGG 
ATGAATTACC TGCGCTACAG CTTGGAGGGG GGCCGCTCCG ACAGTACCAT ACCCAGTTCC 
GACAGGTCTC ACACCCTGAG CCATGCCTCG TACCTGAGGG ACAGTGCCAT GATCGACGAC 
GCAGTTGTGA TCCCCAGTCA CCAGGTGACA ACTCTAGCCA AGGAGGCAGA AAGGAATTCT 
TATCGTCTAA GCTGGGGCAC TGAGAACTTG GACAACGTGG CTCTTTCTTC CAGCCCTATT 
CATTCAGGCC GCACCTCTCC ATGTCTTGAT CGTGACAACA GCAGTTTCCT GGTTAGTTTT 
ATGGTGGATG CCCGAGGTGG TGCTATGCGA GGATGCAGAC ACAACGGGCT CCGCATCATT 
ATTCCGCCCC GGAAATGTAC CGCCCCAACA CGAGTCACCT GCCGACTGGT CAAACGCCAT 
AGACTGGCGA CAATGCCTCC AATGGTGGAA GGAGAGGGCC TGGCCAGTCG CCTGATTGAA 
GTTGGACCTT CTGGTGCTCA GTTCCTTGGG CCTGTGATCG TGGAGATCCC TCATTTCGCT 
GCCCTGCGAG GAAAAGAGAG GGAACTGGTG GTCCTGCGCA GTGAGAACGG GGACAGCTGG 
AAAGAGCATT ACTGTGAATA CACGGAGGAG GAGCTGAATG AAATCCTGAA CGGCATGGAT 
GAAGTGCTGG ATAGCCCGGA AGACCTCGAA AAGAAGCGGA TCTGCCGCAT CATCACCCGT 
GACTTCCCAC AGTACTTTGC GGTGGTGTCG CGCATCAAGC AGGACAGCAA CCTGATTGGC 
CCGGAGGGAG GCGTGCTGAG CAGCACCGTG GTGCCGCAGG TGCAGGCCGT GTTCCCCGAG 
GGGGCGCTAA CCAAGCGGAT CCGTGTTGGC CTACAGGCTC AACCTATGCA CAGTGAGTTG 
GTTAAGAAGA TTCTGGGCAA CAAAGCTACC TTCAGCCCTA TAGTTACTTT GGAACCTAGA 
AGAAGAAAAT TTCACAAGCC AATTACCATG ACCATTCCTG TCCCCAAAGC TTCAAGTGAT 
GTCATGTTGA ATGGTTTTGG AGGAGATGCA CCAACCTTAA GATTACTATG TAGCATCACA 
GGTGGAACCA CCCCTGCTCA GTGGGAAGAT ATTACAGGAA CTACGCCATT AACATTTGTC 
AATGAATGTG TTTCCTTTAC AACCAATGTG TCTGCCAGGT TCTGGCTGAT CGATTGTCGA 
CAAATTCAGG AATCTGTTAC CTTTGCATCA CAAGTGTATA GAGAAATTAT CTGTGTACCA 
TATATGGCCA AATTTGTAGT GTTTGCCAAA TCACATGACC CCATTGAAGC CAGGTTGAGG 
TGTTTCTGCA TGACTGATGA TAAAGTGGAT AAAACCCTGG AACAACAAGA AAACTTTGCT 
GAGGTGGCCA GAAGCAGGGA TGTGGAGGTC TTAGAAGGAA AACCCATCTA TGTTGATTGT 
TTTGGCAACC TGGTGCCATT AACCAAGAGT GGCCAACATC ATATATTCAG TTTTTTTGCC 
TTCAAAGAAA ACAGACTTCC TCTCTTTGTC AAGGTACGTG ATACGACTCA GGAACCTTGC 
GGACGACTAT CATTTATGAA AGAGCCAAAA TCTACAAGAG GCCTGGTGCA CCAAGCTATT 
TGCAACTTAA ACATCACCCT GCCAATTTAT ACGAAGGAAT CAGAGTCAGA TCAAGAACAG 
GAGGAAGAGA TCGATATGAC ATCAGAAAAA AATCAACAGG ATGAGCAGGA ACGGATTGAG 
GAAAGGCTGG CATATATTGC TGATCACCTT GGCTTCAGCT GGACAGAGTT GGCAAGAGAA 
CTGGACTTCA CTGAAGAGCA AATTCATCAA ATTCGAATTG AGAACCCTAA CTCCCTGCAG 
GATCAGAGTC ATGCGCTGTT GAAGTACTGG CTAGAAAGGG ACGGGAGACA TGCTACAGAT 
ACCAGTCTCG TTGAATGTCT CACCAAGATC AACCGAATGG ATATCGTGCA TCTCATGGAG 
ACCAGCACAG AAGCTCTCCA GGAGCGCATC GGTCATAGCT ATGCAGAGAT TGAGCAGACC 
ATTACACTGG ATCATAGTGA AGGGTTCTCA GTACTCCAAG AGGAGCTTTG CACTGCCCAG 
CACAAGCAGA AAGAGGAGCA TGCTGCTTCT AAAGAGGACG AGGCCTCTGA CCACCCTCCC 
ATCGTCTCAG AGGAAGACAT TTCTGTTGGT TACTCCACTC TTCAGGATGG CATCCCCAAA 
ACTGAGGGTG ATGGCTCTGC AACAGAGCTC CTTCCCCAGA CTCAAAAGGA GCAAGTTCAA 
CAGGATTTCT CAGGGAAAAT GCAAGACCTG CCTGAGGAGT CGGCTCTTGA ACAGCAAGAA 
TACTTCGTGA CAACCCCAGG GACAGAAATG TCAGAGACTC AGAAGGCCAC GGGAACATCG 
GGCTCCCCCA CCAAGACACC TGAGGAAATT GTCACCCCTT CCGAGGAGGA GAGGCTGTAT 
CTCCAAACCC CAACATCGAG TGAGCGGGGA GGCTCTCCAG TTGTGCAGGA ACCTGAGGAG 
CCCCCCGAAC GCAGGGAGGA GAGTTCACCT CGGAAAACCA GCCTCGTGAT CGTGGAATCG 
GCTGAGGACC AGCCTCAGGC CTTCGAGAGG CTTGATGAAG AGGCTGCTTT TCAAAAGGGA 
GATGATATGC CTGACATACC CCCAGAAACA GTCACAGAAG AAGAATACAT CGATGAGCAT 
GGACACACCG TGGTAAAGAA GGTTACTAGG AAAATCATTA GGCGGTTCAT CTCCTCTGAC 
GGCACAGAGA AAGAGGAGAT TACGATGCAG GGAGCACCAC AGGATTCTAT CAGCGTTGAT 
GAAGGGGATG GTTATTCCAA AGTCATAAAG CGCATTGTAT TGAAGAGTGA CACCGAGCAG 
TCAGAGGTAA CTTTGTCTGA GCCCAGCATT CTGTCCAGTA CCTCACAATT TCAGGCTGAA 
CCAGTGGAAG GCCGTAGAGT CAGCAAAGTT GTTAAGACAA CTGTGGTCCA TGGTGAGAGG 
ATGGAGAAAC ATCTGGGGGA TTCTAGTTTA GCCACTGATC TTCCTTCAGC CAAAGATGAC 
TTTGAAGAGG CTTTGAGTTA CACAGGTAGC CACATGAAAG TCTACTCCCC CAGTTTAGTA 
GAGAATGAAG TCCTGAAGGA GGATGGATCA ATAATTAGAA GGACGACAAT GAGTAAAGCT 
AGAACCCAGA AGAGGGCTGT GGTGAAGGAC CAGCAGGGAA AACGCATTTA CTTGGAGCAC 
CTGGAGGATG TCCCAGAAGC GCTAGACCAG GACGACCTCC AGCACGACCT CCAGCAACTC 
CTTCGGCATT TCTGCAAGGA GGACTCGAAG CAAGAGGCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849255.1
CDS	239..6283
Translation	MGNAALCPACHADKSVRAQGHLQELDRTPDYYGCNGEDTKELGPRSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSAVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHAMAKNGYTPLHIAAKKNQMQIASTLLSYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMTEVLDVSDEEGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSTIPSSDRSHTLSHASYLRDSAMIDDAVVIPSHQVTTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGRTSPCLDRDNSSFLVSFMVDARGGAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHYCEYTEEELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEIDMTSEKNQQDEQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGRHATDTSLVECLTKINRMDIVHLMETSTEALQERIGHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEHAASKEDEASDHPPIVSEEDISVGYSTLQDGIPKTEGDGSATELLPQTQKEQVQQDFSGKMQDLPEESALEQQEYFVTTPGTEMSETQKATGTSGSPTKTPEEIVTPSEEERLYLQTPTSSERGGSPVVQEPEEPPERREESSPRKTSLVIVESAEDQPQAFERLDEEAAFQKGDDMPDIPPETVTEEEYIDEHGHTVVKKVTRKIIRRFISSDGTEKEEITMQGAPQDSISVDEGDGYSKVIKRIVLKSDTEQSEVTLSEPSILSSTSQFQAEPVEGRRVSKVVKTTVVHGERMEKHLGDSSLATDLPSAKDDFEEALSYTGSHMKVYSPSLVENEVLKEDGSIIRRTTMSKARTQKRAVVKDQQGKRIYLEHLEDVPEALDQDDLQHDLQQLLRHFCKEDSKQEAQ

Target ORF information:

RefSeq Version	XM_024993487.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin 2 (ANK2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993487.1

ORF Insert Sequence:

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
ATGATGGTGA ATAGGACCAC CGAGAGTGGA TTCACCCCTT TGCACATCGC TGCCCACTAT 
GGAAACGTCA ACGTGGCAAC TCTCCTTCTA AACCGGGGAG CTGCTGTGGA CTTCACAGCC 
AGGAATGGAA TCACTCCTCT GCATGTGGCC TCCAAAAGGG GAAACACAAA CATGGTGAAG 
CTCTTACTGG ATCGTGGTGG TCAGATTGAT GCCAAAACTA GGGATGGGTT GACACCCCTT 
CATTGTGCTG CAAGAAGCGG TCATGACCAA GTGGTGGAGC TTCTGCTGGA GCGGGGCGCT 
CCCTTGCTGG CAAGGACGAA GAACGGGCTG TCTCCACTTC ACATGGCTGC ACAGGGGGAC 
CACGTGGAAT GTGTGAAGCA CCTGCTCCAG CACAAGGCGC CTGTTGATGA CGTCACTTTG 
GACTACTTGA CGGCCCTCCA CGTTGCCGCA CACTGCGGCC ACTACCGAGT AACCAAACTT 
CTTCTGGACA AGAGAGCCAA CCCGAACGCC AGAGCCCTGA ATGGTTTTAC TCCACTGCAC 
ATTGCCTGCA AGAAAAACCG CATCAAAGTC ATGGAACTGC TGGTGAAATA TGGGGCTTCA 
ATCCAAGCTA TAACAGAGTC TGGCCTCACA CCAATACATG TGGCTGCCTT CATGGGCCAC 
TTGAACATTG TCCTCCTTCT GCTGCAGAAC GGAGCCTCTC CAGATGTCAC TAACATTCGT 
GGGGAGACGG CCCTGCACAT GGCGGCCCGT GCAGGGCAGG TGGAAGTGGT CCGATGCCTC 
CTGAGGAACG GTGCCCTCGT CGATGCCAGA GCCAGGGAGG AACAGACGCC TTTGCATATT 
GCCTCCCGCC TGGGTAAGAC AGAAATTGTT CAGCTGCTTC TGCAACATAT GGCTCATCCA 
GATGCGGCCA CTACAAATGG GTACACGCCG TTGCATATCT CTGCAAGGGA AGGACAGGTG 
GACGTGGCCT CGGTCCTCCT GGAAGCGGGG GCAGCCCACT CCTTGGCTAC CAAGAAGGGT 
TTTACCCCCT TGCACGTAGC TGCCAAGTAT GGGAGCCTGG ATGTGGCAAA ACTTCTCTTG 
CAACGCCGTG CTGCCGCAGA TTCTGCGGGG AAGAATGGCC TTACCCCGCT CCATGTTGCT 
GCTCACTATG ACAACCAGAA GGTGGCGCTG CTGTTACTGG AGAAGGGTGC TTCCCCTCAC 
GCCATGGCCA AGAATGGCTA CACTCCTTTA CATATTGCTG CCAAGAAGAA TCAAATGCAG 
ATAGCTTCCA CACTCCTGAG CTACGGAGCA GAGACGAACA TCGTGACAAA GCAAGGAGTC 
ACTCCACTCC ATCTGGCCTC GCAGGAGGGA CACACGGATA TGGTCACCTT GCTTCTGGAT 
AAGGGAGCCA ATATCCACAT GTCAACCAAG AGTGGACTCA CATCTTTACA CCTTGCAGCC 
CAGGAAGATA AAGTGAATGT CGCTGATATT CTCACCAAGC ATGGGGCTGA CCAGGATGCG 
CACACAAAGC TTGGTTACAC TCCTTTAATT GTGGCCTGTC ACTATGGAAA CGTGAAAATG 
GTCAACTTCC TTCTGAAGCA GGGAGCAAAT GTCAATGCAA AAACCAAGAA TGGCTACACG 
CCTTTGCACC AAGCCGCCCA ACAGGGCCAC ACGCACATCA TCAATGTTTT GCTGCAGCAC 
GGGGCCAAGC CCAACGCTAC CACCGCGAAT GGCAACACTG CCTTGGCGAT TGCTAAGCGT 
CTGGGCTACA TCTCGGTGGT GGACACCCTG AAGGTTGTAA CGGAGGAGGT CACCACCACC 
ACCACGACTA TCACTGAAAA ACACAAGCTA AATGTTCCTG AGACCATGAC TGAGGTTCTT 
GATGTTTCTG ATGAAGAGGG TGATGACACA ATGACGGGTG ATGGGGGAGA GTACCTTAGA 
CCTGAGGACC TAAAGGAGCT GGGTGATGAC TCCCTCCCCA GCAGTCAGTT CCTGGACGGG 
ATGAATTACC TGCGCTACAG CTTGGAGGGG GGCCGCTCCG ACAGTACCAT ACCCAGTTCC 
GACAGGTCTC ACACCCTGAG CCATGCCTCG TACCTGAGGG ACAGTGCCAT GATCGACGAC 
GCAGTTGTGA TCCCCAGTCA CCAGGTGACA ACTCTAGCCA AGGAGGCAGA AAGGAATTCT 
TATCGTCTAA GCTGGGGCAC TGAGAACTTG GACAACGTGG CTCTTTCTTC CAGCCCTATT 
CATTCAGGCC GCACCTCTCC ATGTCTTGAT CGTGACAACA GCAGTTTCCT GGTTAGTTTT 
ATGGTGGATG CCCGAGGTGG TGCTATGCGA GGATGCAGAC ACAACGGGCT CCGCATCATT 
ATTCCGCCCC GGAAATGTAC CGCCCCAACA CGAGTCACCT GCCGACTGGT CAAACGCCAT 
AGACTGGCGA CAATGCCTCC AATGGTGGAA GGAGAGGGCC TGGCCAGTCG CCTGATTGAA 
GTTGGACCTT CTGGTGCTCA GTTCCTTGGG CCTGTGATCG TGGAGATCCC TCATTTCGCT 
GCCCTGCGAG GAAAAGAGAG GGAACTGGTG GTCCTGCGCA GTGAGAACGG GGACAGCTGG 
AAAGAGCATT ACTGTGAATA CACGGAGGAG GAGCTGAATG AAATCCTGAA CGGCATGGAT 
GAAGTGCTGG ATAGCCCGGA AGACCTCGAA AAGAAGCGGA TCTGCCGCAT CATCACCCGT 
GACTTCCCAC AGTACTTTGC GGTGGTGTCG CGCATCAAGC AGGACAGCAA CCTGATTGGC 
CCGGAGGGAG GCGTGCTGAG CAGCACCGTG GTGCCGCAGG TGCAGGCCGT GTTCCCCGAG 
GGGGCGCTAA CCAAGCGGAT CCGTGTTGGC CTACAGGCTC AACCTATGCA CAGTGAGTTG 
GTTAAGAAGA TTCTGGGCAA CAAAGCTACC TTCAGCCCTA TAGTTACTTT GGAACCTAGA 
AGAAGAAAAT TTCACAAGCC AATTACCATG ACCATTCCTG TCCCCAAAGC TTCAAGTGAT 
GTCATGTTGA ATGGTTTTGG AGGAGATGCA CCAACCTTAA GATTACTATG TAGCATCACA 
GGTGGAACCA CCCCTGCTCA GTGGGAAGAT ATTACAGGAA CTACGCCATT AACATTTGTC 
AATGAATGTG TTTCCTTTAC AACCAATGTG TCTGCCAGGT TCTGGCTGAT CGATTGTCGA 
CAAATTCAGG AATCTGTTAC CTTTGCATCA CAAGTGTATA GAGAAATTAT CTGTGTACCA 
TATATGGCCA AATTTGTAGT GTTTGCCAAA TCACATGACC CCATTGAAGC CAGGTTGAGG 
TGTTTCTGCA TGACTGATGA TAAAGTGGAT AAAACCCTGG AACAACAAGA AAACTTTGCT 
GAGGTGGCCA GAAGCAGGGA TGTGGAGGTC TTAGAAGGAA AACCCATCTA TGTTGATTGT 
TTTGGCAACC TGGTGCCATT AACCAAGAGT GGCCAACATC ATATATTCAG TTTTTTTGCC 
TTCAAAGAAA ACAGACTTCC TCTCTTTGTC AAGGTACGTG ATACGACTCA GGAACCTTGC 
GGACGACTAT CATTTATGAA AGAGCCAAAA TCTACAAGAG GCCTGGTGCA CCAAGCTATT 
TGCAACTTAA ACATCACCCT GCCAATTTAT ACGAAGGAAT CAGAGTCAGA TCAAGAACAG 
GAGGAAGAGA TCGATATGAC ATCAGAAAAA AATCAACAGG ATGAGCAGGA ACGGATTGAG 
GAAAGGCTGG CATATATTGC TGATCACCTT GGCTTCAGCT GGACAGAGTT GGCAAGAGAA 
CTGGACTTCA CTGAAGAGCA AATTCATCAA ATTCGAATTG AGAACCCTAA CTCCCTGCAG 
GATCAGAGTC ATGCGCTGTT GAAGTACTGG CTAGAAAGGG ACGGGAGACA TGCTACAGAT 
ACCAGTCTCG TTGAATGTCT CACCAAGATC AACCGAATGG ATATCGTGCA TCTCATGGAG 
ACCAGCACAG AAGCTCTCCA GGAGCGCATC GGTCATAGCT ATGCAGAGAT TGAGCAGACC 
ATTACACTGG ATCATAGTGA AGGGTTCTCA GTACTCCAAG AGGAGCTTTG CACTGCCCAG 
CACAAGCAGA AAGAGGAGCA TGCTGCTTCT AAAGAGGACG AGGCCTCTGA CCACCCTCCC 
ATCGTCTCAG AGGAAGACAT TTCTGTTGGT TACTCCACTC TTCAGGATGG CATCCCCAAA 
ACTGAGGGTG ATGGCTCTGC AACAGAGCTC CTTCCCCAGA CTCAAAAGGA GCAAGTTCAA 
CAGGATTTCT CAGGGAAAAT GCAAGACCTG CCTGAGGAGT CGGCTCTTGA ACAGCAAGAA 
TACTTCGTGA CAACCCCAGG GACAGAAATG TCAGAGACTC AGAAGGCCAC GGGAACATCG 
GGCTCCCCCA CCAAGACACC TGAGGAAATT GTCACCCCTT CCGAGGAGGA GAGGCTGTAT 
CTCCAAACCC CAACATCGAG TGAGCGGGGA GGCTCTCCAG TTGTGCAGGA ACCTGAGGAG 
CCCCCCGAAC GCAGGGAGGA GAGTTCACCT CGGAAAACCA GCCTCGTGAT CGTGGAATCG 
GCTGAGGACC AGCCTCAGGC CTTCGAGAGG CTTGATGAAG AGGCTGCTTT TCAAAAGGGA 
GATGATATGC CTGACATACC CCCAGAAACA GTCACAGAAG AAGAATACAT CGATGAGCAT 
GGACACACCG TGGTAAAGAA GGTTACTAGG AAAATCATTA GGCGGTTCAT CTCCTCTGAC 
GGCACAGAGA AAGAGGAGAT TACGATGCAG GGAGCACCAC AGGATTCTAT CAGCGTTGAT 
GAAGGGGATG GTTATTCCAA AGTCATAAAG CGCATTGTAT TGAAGAGTGA CACCGAGCAG 
TCAGAGGTAA CTTTGTCTGA GCCCAGCATT CTGTCCAGTA CCTCACAATT TCAGGCTGAA 
CCAGTGGAAG GCCGTAGAGT CAGCAAAGTT GTTAAGACAA CTGTGGTCCA TGGTGAGAGG 
ATGGAGAAAC ATCTGGGGGA TTCTAGTTTA GCCACTGATC TTCCTTCAGC CAAAGATGAC 
TTTGAAGAGG CTTTGAGTTA CACAGGTAGC CACATGAAAG TCTACTCCCC CAGTTTAGTA 
GAGAATGAAG TCCTGAAGGA GGATGGATCA ATAATTAGAA GGACGACAAT GAGTAAAGCT 
AGAACCCAGA AGAGGGCTGT GGTGAAGGAC CAGCAGGGAA AACGCATTTA CTTGGAGCAC 
CTGGAGGATG TCCCAGAAGC GCTAGACCAG GACGACCTCC AGCACGACCT CCAGCAACTC 
CTTCGGCATT TCTGCAAGGA GGACTCGAAG CAAGAGGCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK2 ( XM_024993488.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849256.1 Bos taurus ankyrin 2 (ANK2), transcript variant X4, mRNA.

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CloneID	OBa235480
Clone ID Related Accession (Same CDS sequence)	XM_024993488.1
Accession Version	XM_024993488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
AGTGGATTCA CCCCTTTGCA CATCGCTGCC CACTATGGAA ACGTCAACGT GGCAACTCTC 
CTTCTAAACC GGGGAGCTGC TGTGGACTTC ACAGCCAGGA ATGGAATCAC TCCTCTGCAT 
GTGGCCTCCA AAAGGGGAAA CACAAACATG GTGAAGCTCT TACTGGATCG TGGTGGTCAG 
ATTGATGCCA AAACTAGGGA TGGGTTGACA CCCCTTCATT GTGCTGCAAG AAGCGGTCAT 
GACCAAGTGG TGGAGCTTCT GCTGGAGCGG GGCGCTCCCT TGCTGGCAAG GACGAAGAAC 
GGGCTGTCTC CACTTCACAT GGCTGCACAG GGGGACCACG TGGAATGTGT GAAGCACCTG 
CTCCAGCACA AGGCGCCTGT TGATGACGTC ACTTTGGACT ACTTGACGGC CCTCCACGTT 
GCCGCACACT GCGGCCACTA CCGAGTAACC AAACTTCTTC TGGACAAGAG AGCCAACCCG 
AACGCCAGAG CCCTGAATGG TTTTACTCCA CTGCACATTG CCTGCAAGAA AAACCGCATC 
AAAGTCATGG AACTGCTGGT GAAATATGGG GCTTCAATCC AAGCTATAAC AGAGTCTGGC 
CTCACACCAA TACATGTGGC TGCCTTCATG GGCCACTTGA ACATTGTCCT CCTTCTGCTG 
CAGAACGGAG CCTCTCCAGA TGTCACTAAC ATTCGTGGGG AGACGGCCCT GCACATGGCG 
GCCCGTGCAG GGCAGGTGGA AGTGGTCCGA TGCCTCCTGA GGAACGGTGC CCTCGTCGAT 
GCCAGAGCCA GGGAGGAACA GACGCCTTTG CATATTGCCT CCCGCCTGGG TAAGACAGAA 
ATTGTTCAGC TGCTTCTGCA ACATATGGCT CATCCAGATG CGGCCACTAC AAATGGGTAC 
ACGCCGTTGC ATATCTCTGC AAGGGAAGGA CAGGTGGACG TGGCCTCGGT CCTCCTGGAA 
GCGGGGGCAG CCCACTCCTT GGCTACCAAG AAGGGTTTTA CCCCCTTGCA CGTAGCTGCC 
AAGTATGGGA GCCTGGATGT GGCAAAACTT CTCTTGCAAC GCCGTGCTGC CGCAGATTCT 
GCGGGGAAGA ATGGCCTTAC CCCGCTCCAT GTTGCTGCTC ACTATGACAA CCAGAAGGTG 
GCGCTGCTGT TACTGGAGAA GGGTGCTTCC CCTCACGCCA TGGCCAAGAA TGGCTACACT 
CCTTTACATA TTGCTGCCAA GAAGAATCAA ATGCAGATAG CTTCCACACT CCTGAGCTAC 
GGAGCAGAGA CGAACATCGT GACAAAGCAA GGAGTCACTC CACTCCATCT GGCCTCGCAG 
GAGGGACACA CGGATATGGT CACCTTGCTT CTGGATAAGG GAGCCAATAT CCACATGTCA 
ACCAAGAGTG GACTCACATC TTTACACCTT GCAGCCCAGG AAGATAAAGT GAATGTCGCT 
GATATTCTCA CCAAGCATGG GGCTGACCAG GATGCGCACA CAAAGCTTGG TTACACTCCT 
TTAATTGTGG CCTGTCACTA TGGAAACGTG AAAATGGTCA ACTTCCTTCT GAAGCAGGGA 
GCAAATGTCA ATGCAAAAAC CAAGAATGGC TACACGCCTT TGCACCAAGC CGCCCAACAG 
GGCCACACGC ACATCATCAA TGTTTTGCTG CAGCACGGGG CCAAGCCCAA CGCTACCACC 
GCGAATGGCA ACACTGCCTT GGCGATTGCT AAGCGTCTGG GCTACATCTC GGTGGTGGAC 
ACCCTGAAGG TTGTAACGGA GGAGGTCACC ACCACCACCA CGACTATCAC TGAAAAACAC 
AAGCTAAATG TTCCTGAGAC CATGACTGAG GTTCTTGATG TTTCTGATGA AGAGGGTGAT 
GACACAATGA CGGGTGATGG GGGAGAGTAC CTTAGACCTG AGGACCTAAA GGAGCTGGGT 
GATGACTCCC TCCCCAGCAG TCAGTTCCTG GACGGGATGA ATTACCTGCG CTACAGCTTG 
GAGGGGGGCC GCTCCGACAG TTCCGACAGG TCTCACACCC TGAGCCATGC CTCGTACCTG 
AGGGACAGTG CCATGATCGA CGACGCAGTT GTGATCCCCA GTCACCAGGT GACAACTCTA 
GCCAAGGAGG CAGAAAGGAA TTCTTATCGT CTAAGCTGGG GCACTGAGAA CTTGGACAAC 
GTGGCTCTTT CTTCCAGCCC TATTCATTCA GGTTTCCTGG TTAGTTTTAT GGTGGATGCC 
CGAGGTGGTG CTATGCGAGG ATGCAGACAC AACGGGCTCC GCATCATTAT TCCGCCCCGG 
AAATGTACCG CCCCAACACG AGTCACCTGC CGACTGGTCA AACGCCATAG ACTGGCGACA 
ATGCCTCCAA TGGTGGAAGG AGAGGGCCTG GCCAGTCGCC TGATTGAAGT TGGACCTTCT 
GGTGCTCAGT TCCTTGGGCC TGTGATCGTG GAGATCCCTC ATTTCGCTGC CCTGCGAGGA 
AAAGAGAGGG AACTGGTGGT CCTGCGCAGT GAGAACGGGG ACAGCTGGAA AGAGCATTAC 
TGTGAATACA CGGAGGAGGA GCTGAATGAA ATCCTGAACG GCATGGATGA AGTGCTGGAT 
AGCCCGGAAG ACCTCGAAAA GAAGCGGATC TGCCGCATCA TCACCCGTGA CTTCCCACAG 
TACTTTGCGG TGGTGTCGCG CATCAAGCAG GACAGCAACC TGATTGGCCC GGAGGGAGGC 
GTGCTGAGCA GCACCGTGGT GCCGCAGGTG CAGGCCGTGT TCCCCGAGGG GGCGCTAACC 
AAGCGGATCC GTGTTGGCCT ACAGGCTCAA CCTATGCACA GTGAGTTGGT TAAGAAGATT 
CTGGGCAACA AAGCTACCTT CAGCCCTATA GTTACTTTGG AACCTAGAAG AAGAAAATTT 
CACAAGCCAA TTACCATGAC CATTCCTGTC CCCAAAGCTT CAAGTGATGT CATGTTGAAT 
GGTTTTGGAG GAGATGCACC AACCTTAAGA TTACTATGTA GCATCACAGG TGGAACCACC 
CCTGCTCAGT GGGAAGATAT TACAGGAACT ACGCCATTAA CATTTGTCAA TGAATGTGTT 
TCCTTTACAA CCAATGTGTC TGCCAGGTTC TGGCTGATCG ATTGTCGACA AATTCAGGAA 
TCTGTTACCT TTGCATCACA AGTGTATAGA GAAATTATCT GTGTACCATA TATGGCCAAA 
TTTGTAGTGT TTGCCAAATC ACATGACCCC ATTGAAGCCA GGTTGAGGTG TTTCTGCATG 
ACTGATGATA AAGTGGATAA AACCCTGGAA CAACAAGAAA ACTTTGCTGA GGTGGCCAGA 
AGCAGGGATG TGGAGGTCTT AGAAGGAAAA CCCATCTATG TTGATTGTTT TGGCAACCTG 
GTGCCATTAA CCAAGAGTGG CCAACATCAT ATATTCAGTT TTTTTGCCTT CAAAGAAAAC 
AGACTTCCTC TCTTTGTCAA GGTACGTGAT ACGACTCAGG AACCTTGCGG ACGACTATCA 
TTTATGAAAG AGCCAAAATC TACAAGAGGC CTGGTGCACC AAGCTATTTG CAACTTAAAC 
ATCACCCTGC CAATTTATAC GAAGATCGAT ATGACATCAG AAAAAAATCA ACAGGATGAG 
CAGGAACGGA TTGAGGAAAG GCTGGCATAT ATTGCTGATC ACCTTGGCTT CAGCTGGACA 
GAGTTGGCAA GAGAACTGGA CTTCACTGAA GAGCAAATTC ATCAAATTCG AATTGAGAAC 
CCTAACTCCC TGCAGGATCA GAGTCATGCG CTGTTGAAGT ACTGGCTAGA AAGGGACGGG 
AGACATGCTA CAGATACCAG TCTCGTTGAA TGTCTCACCA AGATCAACCG AATGGATATC 
GTGCATCTCA TGGAGACCAG CACAGAAGCT CTCCAGGAGC GCATCGGTCA TAGCTATGCA 
GAGATTGAGC AGACCATTAC ACTGGATCAT AGTGAAGGGT TCTCAGTACT CCAAGAGGAG 
CTTTGCACTG CCCAGCACAA GCAGAAAGAG GAGCATGCTG CTTCTAAAGA GGACGAGGCC 
TCTGACCACC CTCCCATCGT CTCAGAGGAA GACATTTCTG TTGGTTACTC CACTCTTCAG 
GATGGCATCC CCAAAACTGA GGGTGATGGC TCTGCAACAG AGCTCCTTCC CCAGACTCAA 
AAGGAGCAAG TTCAACAGGA TTTCTCAGGG AAAATGCAAG ACCTGCCTGA GGAGTCGGCT 
CTTGAACAGC AAGAATACTT CGTGACAACC CCAGGGACAG AAATGTCAGA GACTCAGAAG 
GCCACGGGAA CATCGGGCTC CCCCACCAAG ACACCTGAGG AAATTGTCAC CCCTTCCGAG 
GAGGAGAGGC TGTATCTCCA AACCCCAACA TCGAGTGAGC GGGGAGGCTC TCCAGTTGTG 
CAGGAACCTG AGGAGCCCCC CGAACGCAGG GAGGAGAGTT CACCTCGGAA AACCAGCCTC 
GTGATCGTGG AATCGGCTGA GGACCAGCCT CAGGCCTTCG AGAGGCTTGA TGAAGAGGCT 
GCTTTTCAAA AGGAGCTAAC AGAGGAATTA GGTGAGCTGG AGGCCAGCTC AGATGAGGAG 
GCGATGGTAA CAACCAGGGT TGTCCGCCGA CGATTGATTA TTCAGGGAGA TGATATGCCT 
GACATACCCC CAGAAACAGT CACAGAAGAA GAATACATCG ATGAGCATGG ACACACCGTG 
GTAAAGAAGG TTACTAGGAA AATCATTAGG CGGTTCATCT CCTCTGACGG CACAGAGAAA 
GAGGAGATTA CGATGCAGGG AGCACCACAG GATTCTATCA GCGTTGATGA AGGGGATGGT 
TATTCCAAAG TCATAAAGCG CATTGTATTG AAGAGTGACA CCGAGCAGTC AGAGGTAACT 
TTGTCTGAGC CCAGCATTCT GTCCAGTACC TCACAATTTC AGGCTGAACC AGTGGAAGGC 
CGTAGAGTCA GCAAAGTTGT TAAGACAACT GTGGTCCATG GTGAGAGGAT GGAGAAACAT 
CTGGGGGATT CTAGTTTAGC CACTGATCTT CCTTCAGCCA AAGATGACTT TGAAGAGGCT 
TTGAGTTACA CAGGTAGCCA CATGAAAGTC TACTCCCCCA GTTTAGTAGA GAATGAAGTC 
CTGAAGGAGG ATGGATCAAT AATTAGAAGG ACGACAATGA GTAAAGCTAG AACCCAGAAG 
AGGGCTGTGG TGAAGGACCA GCAGGGAAAA CGCATTTACT TGGAGCACCT GGAGGATGTC 
CCAGAAGCGC TAGACCAGGA CGACCTCCAG CACGACCTCC AGCAACTCCT TCGGCATTTC 
TGCAAGGAGG ACTCGAAGCA AGAGGCCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849256.1
CDS	239..6271
Translation	MGNAALCPACHADKSVRAQGHLQELDRTPDYYGCNGEDTKELGPRSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSAVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHAMAKNGYTPLHIAAKKNQMQIASTLLSYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMTEVLDVSDEEGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSSDRSHTLSHASYLRDSAMIDDAVVIPSHQVTTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGFLVSFMVDARGGAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHYCEYTEEELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKIDMTSEKNQQDEQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGRHATDTSLVECLTKINRMDIVHLMETSTEALQERIGHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEHAASKEDEASDHPPIVSEEDISVGYSTLQDGIPKTEGDGSATELLPQTQKEQVQQDFSGKMQDLPEESALEQQEYFVTTPGTEMSETQKATGTSGSPTKTPEEIVTPSEEERLYLQTPTSSERGGSPVVQEPEEPPERREESSPRKTSLVIVESAEDQPQAFERLDEEAAFQKELTEELGELEASSDEEAMVTTRVVRRRLIIQGDDMPDIPPETVTEEEYIDEHGHTVVKKVTRKIIRRFISSDGTEKEEITMQGAPQDSISVDEGDGYSKVIKRIVLKSDTEQSEVTLSEPSILSSTSQFQAEPVEGRRVSKVVKTTVVHGERMEKHLGDSSLATDLPSAKDDFEEALSYTGSHMKVYSPSLVENEVLKEDGSIIRRTTMSKARTQKRAVVKDQQGKRIYLEHLEDVPEALDQDDLQHDLQQLLRHFCKEDSKQEAQ

Target ORF information:

RefSeq Version	XM_024993488.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin 2 (ANK2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993488.1

ORF Insert Sequence:

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
AGTGGATTCA CCCCTTTGCA CATCGCTGCC CACTATGGAA ACGTCAACGT GGCAACTCTC 
CTTCTAAACC GGGGAGCTGC TGTGGACTTC ACAGCCAGGA ATGGAATCAC TCCTCTGCAT 
GTGGCCTCCA AAAGGGGAAA CACAAACATG GTGAAGCTCT TACTGGATCG TGGTGGTCAG 
ATTGATGCCA AAACTAGGGA TGGGTTGACA CCCCTTCATT GTGCTGCAAG AAGCGGTCAT 
GACCAAGTGG TGGAGCTTCT GCTGGAGCGG GGCGCTCCCT TGCTGGCAAG GACGAAGAAC 
GGGCTGTCTC CACTTCACAT GGCTGCACAG GGGGACCACG TGGAATGTGT GAAGCACCTG 
CTCCAGCACA AGGCGCCTGT TGATGACGTC ACTTTGGACT ACTTGACGGC CCTCCACGTT 
GCCGCACACT GCGGCCACTA CCGAGTAACC AAACTTCTTC TGGACAAGAG AGCCAACCCG 
AACGCCAGAG CCCTGAATGG TTTTACTCCA CTGCACATTG CCTGCAAGAA AAACCGCATC 
AAAGTCATGG AACTGCTGGT GAAATATGGG GCTTCAATCC AAGCTATAAC AGAGTCTGGC 
CTCACACCAA TACATGTGGC TGCCTTCATG GGCCACTTGA ACATTGTCCT CCTTCTGCTG 
CAGAACGGAG CCTCTCCAGA TGTCACTAAC ATTCGTGGGG AGACGGCCCT GCACATGGCG 
GCCCGTGCAG GGCAGGTGGA AGTGGTCCGA TGCCTCCTGA GGAACGGTGC CCTCGTCGAT 
GCCAGAGCCA GGGAGGAACA GACGCCTTTG CATATTGCCT CCCGCCTGGG TAAGACAGAA 
ATTGTTCAGC TGCTTCTGCA ACATATGGCT CATCCAGATG CGGCCACTAC AAATGGGTAC 
ACGCCGTTGC ATATCTCTGC AAGGGAAGGA CAGGTGGACG TGGCCTCGGT CCTCCTGGAA 
GCGGGGGCAG CCCACTCCTT GGCTACCAAG AAGGGTTTTA CCCCCTTGCA CGTAGCTGCC 
AAGTATGGGA GCCTGGATGT GGCAAAACTT CTCTTGCAAC GCCGTGCTGC CGCAGATTCT 
GCGGGGAAGA ATGGCCTTAC CCCGCTCCAT GTTGCTGCTC ACTATGACAA CCAGAAGGTG 
GCGCTGCTGT TACTGGAGAA GGGTGCTTCC CCTCACGCCA TGGCCAAGAA TGGCTACACT 
CCTTTACATA TTGCTGCCAA GAAGAATCAA ATGCAGATAG CTTCCACACT CCTGAGCTAC 
GGAGCAGAGA CGAACATCGT GACAAAGCAA GGAGTCACTC CACTCCATCT GGCCTCGCAG 
GAGGGACACA CGGATATGGT CACCTTGCTT CTGGATAAGG GAGCCAATAT CCACATGTCA 
ACCAAGAGTG GACTCACATC TTTACACCTT GCAGCCCAGG AAGATAAAGT GAATGTCGCT 
GATATTCTCA CCAAGCATGG GGCTGACCAG GATGCGCACA CAAAGCTTGG TTACACTCCT 
TTAATTGTGG CCTGTCACTA TGGAAACGTG AAAATGGTCA ACTTCCTTCT GAAGCAGGGA 
GCAAATGTCA ATGCAAAAAC CAAGAATGGC TACACGCCTT TGCACCAAGC CGCCCAACAG 
GGCCACACGC ACATCATCAA TGTTTTGCTG CAGCACGGGG CCAAGCCCAA CGCTACCACC 
GCGAATGGCA ACACTGCCTT GGCGATTGCT AAGCGTCTGG GCTACATCTC GGTGGTGGAC 
ACCCTGAAGG TTGTAACGGA GGAGGTCACC ACCACCACCA CGACTATCAC TGAAAAACAC 
AAGCTAAATG TTCCTGAGAC CATGACTGAG GTTCTTGATG TTTCTGATGA AGAGGGTGAT 
GACACAATGA CGGGTGATGG GGGAGAGTAC CTTAGACCTG AGGACCTAAA GGAGCTGGGT 
GATGACTCCC TCCCCAGCAG TCAGTTCCTG GACGGGATGA ATTACCTGCG CTACAGCTTG 
GAGGGGGGCC GCTCCGACAG TTCCGACAGG TCTCACACCC TGAGCCATGC CTCGTACCTG 
AGGGACAGTG CCATGATCGA CGACGCAGTT GTGATCCCCA GTCACCAGGT GACAACTCTA 
GCCAAGGAGG CAGAAAGGAA TTCTTATCGT CTAAGCTGGG GCACTGAGAA CTTGGACAAC 
GTGGCTCTTT CTTCCAGCCC TATTCATTCA GGTTTCCTGG TTAGTTTTAT GGTGGATGCC 
CGAGGTGGTG CTATGCGAGG ATGCAGACAC AACGGGCTCC GCATCATTAT TCCGCCCCGG 
AAATGTACCG CCCCAACACG AGTCACCTGC CGACTGGTCA AACGCCATAG ACTGGCGACA 
ATGCCTCCAA TGGTGGAAGG AGAGGGCCTG GCCAGTCGCC TGATTGAAGT TGGACCTTCT 
GGTGCTCAGT TCCTTGGGCC TGTGATCGTG GAGATCCCTC ATTTCGCTGC CCTGCGAGGA 
AAAGAGAGGG AACTGGTGGT CCTGCGCAGT GAGAACGGGG ACAGCTGGAA AGAGCATTAC 
TGTGAATACA CGGAGGAGGA GCTGAATGAA ATCCTGAACG GCATGGATGA AGTGCTGGAT 
AGCCCGGAAG ACCTCGAAAA GAAGCGGATC TGCCGCATCA TCACCCGTGA CTTCCCACAG 
TACTTTGCGG TGGTGTCGCG CATCAAGCAG GACAGCAACC TGATTGGCCC GGAGGGAGGC 
GTGCTGAGCA GCACCGTGGT GCCGCAGGTG CAGGCCGTGT TCCCCGAGGG GGCGCTAACC 
AAGCGGATCC GTGTTGGCCT ACAGGCTCAA CCTATGCACA GTGAGTTGGT TAAGAAGATT 
CTGGGCAACA AAGCTACCTT CAGCCCTATA GTTACTTTGG AACCTAGAAG AAGAAAATTT 
CACAAGCCAA TTACCATGAC CATTCCTGTC CCCAAAGCTT CAAGTGATGT CATGTTGAAT 
GGTTTTGGAG GAGATGCACC AACCTTAAGA TTACTATGTA GCATCACAGG TGGAACCACC 
CCTGCTCAGT GGGAAGATAT TACAGGAACT ACGCCATTAA CATTTGTCAA TGAATGTGTT 
TCCTTTACAA CCAATGTGTC TGCCAGGTTC TGGCTGATCG ATTGTCGACA AATTCAGGAA 
TCTGTTACCT TTGCATCACA AGTGTATAGA GAAATTATCT GTGTACCATA TATGGCCAAA 
TTTGTAGTGT TTGCCAAATC ACATGACCCC ATTGAAGCCA GGTTGAGGTG TTTCTGCATG 
ACTGATGATA AAGTGGATAA AACCCTGGAA CAACAAGAAA ACTTTGCTGA GGTGGCCAGA 
AGCAGGGATG TGGAGGTCTT AGAAGGAAAA CCCATCTATG TTGATTGTTT TGGCAACCTG 
GTGCCATTAA CCAAGAGTGG CCAACATCAT ATATTCAGTT TTTTTGCCTT CAAAGAAAAC 
AGACTTCCTC TCTTTGTCAA GGTACGTGAT ACGACTCAGG AACCTTGCGG ACGACTATCA 
TTTATGAAAG AGCCAAAATC TACAAGAGGC CTGGTGCACC AAGCTATTTG CAACTTAAAC 
ATCACCCTGC CAATTTATAC GAAGATCGAT ATGACATCAG AAAAAAATCA ACAGGATGAG 
CAGGAACGGA TTGAGGAAAG GCTGGCATAT ATTGCTGATC ACCTTGGCTT CAGCTGGACA 
GAGTTGGCAA GAGAACTGGA CTTCACTGAA GAGCAAATTC ATCAAATTCG AATTGAGAAC 
CCTAACTCCC TGCAGGATCA GAGTCATGCG CTGTTGAAGT ACTGGCTAGA AAGGGACGGG 
AGACATGCTA CAGATACCAG TCTCGTTGAA TGTCTCACCA AGATCAACCG AATGGATATC 
GTGCATCTCA TGGAGACCAG CACAGAAGCT CTCCAGGAGC GCATCGGTCA TAGCTATGCA 
GAGATTGAGC AGACCATTAC ACTGGATCAT AGTGAAGGGT TCTCAGTACT CCAAGAGGAG 
CTTTGCACTG CCCAGCACAA GCAGAAAGAG GAGCATGCTG CTTCTAAAGA GGACGAGGCC 
TCTGACCACC CTCCCATCGT CTCAGAGGAA GACATTTCTG TTGGTTACTC CACTCTTCAG 
GATGGCATCC CCAAAACTGA GGGTGATGGC TCTGCAACAG AGCTCCTTCC CCAGACTCAA 
AAGGAGCAAG TTCAACAGGA TTTCTCAGGG AAAATGCAAG ACCTGCCTGA GGAGTCGGCT 
CTTGAACAGC AAGAATACTT CGTGACAACC CCAGGGACAG AAATGTCAGA GACTCAGAAG 
GCCACGGGAA CATCGGGCTC CCCCACCAAG ACACCTGAGG AAATTGTCAC CCCTTCCGAG 
GAGGAGAGGC TGTATCTCCA AACCCCAACA TCGAGTGAGC GGGGAGGCTC TCCAGTTGTG 
CAGGAACCTG AGGAGCCCCC CGAACGCAGG GAGGAGAGTT CACCTCGGAA AACCAGCCTC 
GTGATCGTGG AATCGGCTGA GGACCAGCCT CAGGCCTTCG AGAGGCTTGA TGAAGAGGCT 
GCTTTTCAAA AGGAGCTAAC AGAGGAATTA GGTGAGCTGG AGGCCAGCTC AGATGAGGAG 
GCGATGGTAA CAACCAGGGT TGTCCGCCGA CGATTGATTA TTCAGGGAGA TGATATGCCT 
GACATACCCC CAGAAACAGT CACAGAAGAA GAATACATCG ATGAGCATGG ACACACCGTG 
GTAAAGAAGG TTACTAGGAA AATCATTAGG CGGTTCATCT CCTCTGACGG CACAGAGAAA 
GAGGAGATTA CGATGCAGGG AGCACCACAG GATTCTATCA GCGTTGATGA AGGGGATGGT 
TATTCCAAAG TCATAAAGCG CATTGTATTG AAGAGTGACA CCGAGCAGTC AGAGGTAACT 
TTGTCTGAGC CCAGCATTCT GTCCAGTACC TCACAATTTC AGGCTGAACC AGTGGAAGGC 
CGTAGAGTCA GCAAAGTTGT TAAGACAACT GTGGTCCATG GTGAGAGGAT GGAGAAACAT 
CTGGGGGATT CTAGTTTAGC CACTGATCTT CCTTCAGCCA AAGATGACTT TGAAGAGGCT 
TTGAGTTACA CAGGTAGCCA CATGAAAGTC TACTCCCCCA GTTTAGTAGA GAATGAAGTC 
CTGAAGGAGG ATGGATCAAT AATTAGAAGG ACGACAATGA GTAAAGCTAG AACCCAGAAG 
AGGGCTGTGG TGAAGGACCA GCAGGGAAAA CGCATTTACT TGGAGCACCT GGAGGATGTC 
CCAGAAGCGC TAGACCAGGA CGACCTCCAG CACGACCTCC AGCAACTCCT TCGGCATTTC 
TGCAAGGAGG ACTCGAAGCA AGAGGCCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK2 ( XM_024993489.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849257.1 Bos taurus ankyrin 2 (ANK2), transcript variant X5, mRNA.

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CloneID	OBa235481
Clone ID Related Accession (Same CDS sequence)	XM_024993489.1
Accession Version	XM_024993489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
ATGATGGTGA ATAGGACCAC CGAGAGTGGA TTCACCCCTT TGCACATCGC TGCCCACTAT 
GGAAACGTCA ACGTGGCAAC TCTCCTTCTA AACCGGGGAG CTGCTGTGGA CTTCACAGCC 
AGGAATGGAA TCACTCCTCT GCATGTGGCC TCCAAAAGGG GAAACACAAA CATGGTGAAG 
CTCTTACTGG ATCGTGGTGG TCAGATTGAT GCCAAAACTA GGGATGGGTT GACACCCCTT 
CATTGTGCTG CAAGAAGCGG TCATGACCAA GTGGTGGAGC TTCTGCTGGA GCGGGGCGCT 
CCCTTGCTGG CAAGGACGAA GAACGGGCTG TCTCCACTTC ACATGGCTGC ACAGGGGGAC 
CACGTGGAAT GTGTGAAGCA CCTGCTCCAG CACAAGGCGC CTGTTGATGA CGTCACTTTG 
GACTACTTGA CGGCCCTCCA CGTTGCCGCA CACTGCGGCC ACTACCGAGT AACCAAACTT 
CTTCTGGACA AGAGAGCCAA CCCGAACGCC AGAGCCCTGA ATGGTTTTAC TCCACTGCAC 
ATTGCCTGCA AGAAAAACCG CATCAAAGTC ATGGAACTGC TGGTGAAATA TGGGGCTTCA 
ATCCAAGCTA TAACAGAGTC TGGCCTCACA CCAATACATG TGGCTGCCTT CATGGGCCAC 
TTGAACATTG TCCTCCTTCT GCTGCAGAAC GGAGCCTCTC CAGATGTCAC TAACATTCGT 
GGGGAGACGG CCCTGCACAT GGCGGCCCGT GCAGGGCAGG TGGAAGTGGT CCGATGCCTC 
CTGAGGAACG GTGCCCTCGT CGATGCCAGA GCCAGGGAGG AACAGACGCC TTTGCATATT 
GCCTCCCGCC TGGGTAAGAC AGAAATTGTT CAGCTGCTTC TGCAACATAT GGCTCATCCA 
GATGCGGCCA CTACAAATGG GTACACGCCG TTGCATATCT CTGCAAGGGA AGGACAGGTG 
GACGTGGCCT CGGTCCTCCT GGAAGCGGGG GCAGCCCACT CCTTGGCTAC CAAGAAGGGT 
TTTACCCCCT TGCACGTAGC TGCCAAGTAT GGGAGCCTGG ATGTGGCAAA ACTTCTCTTG 
CAACGCCGTG CTGCCGCAGA TTCTGCGGGG AAGAATGGCC TTACCCCGCT CCATGTTGCT 
GCTCACTATG ACAACCAGAA GGTGGCGCTG CTGTTACTGG AGAAGGGTGC TTCCCCTCAC 
GCCATGGCCA AGAATGGCTA CACTCCTTTA CATATTGCTG CCAAGAAGAA TCAAATGCAG 
ATAGCTTCCA CACTCCTGAG CTACGGAGCA GAGACGAACA TCGTGACAAA GCAAGGAGTC 
ACTCCACTCC ATCTGGCCTC GCAGGAGGGA CACACGGATA TGGTCACCTT GCTTCTGGAT 
AAGGGAGCCA ATATCCACAT GTCAACCAAG AGTGGACTCA CATCTTTACA CCTTGCAGCC 
CAGGAAGATA AAGTGAATGT CGCTGATATT CTCACCAAGC ATGGGGCTGA CCAGGATGCG 
CACACAAAGC TTGGTTACAC TCCTTTAATT GTGGCCTGTC ACTATGGAAA CGTGAAAATG 
GTCAACTTCC TTCTGAAGCA GGGAGCAAAT GTCAATGCAA AAACCAAGAA TGGCTACACG 
CCTTTGCACC AAGCCGCCCA ACAGGGCCAC ACGCACATCA TCAATGTTTT GCTGCAGCAC 
GGGGCCAAGC CCAACGCTAC CACCGCGAAT GGCAACACTG CCTTGGCGAT TGCTAAGCGT 
CTGGGCTACA TCTCGGTGGT GGACACCCTG AAGGTTGTAA CGGAGGAGGT CACCACCACC 
ACCACGACTA TCACTGAAAA ACACAAGCTA AATGTTCCTG AGACCATGAC TGAGGTTCTT 
GATGTTTCTG ATGAAGAGGG TGATGACACA ATGACGGGTG ATGGGGGAGA GTACCTTAGA 
CCTGAGGACC TAAAGGAGCT GGGTGATGAC TCCCTCCCCA GCAGTCAGTT CCTGGACGGG 
ATGAATTACC TGCGCTACAG CTTGGAGGGG GGCCGCTCCG ACAGCCTTCG ATCCTTCAGT 
TCCGACAGGT CTCACACCCT GAGCCATGCC TCGTACCTGA GGGACAGTGC CATGATCGAC 
GACGCAGTTG TGATCCCCAG TCACCAGGTG ACAACTCTAG CCAAGGAGGC AGAAAGGAAT 
TCTTATCGTC TAAGCTGGGG CACTGAGAAC TTGGACAACG TGGCTCTTTC TTCCAGCCCT 
ATTCATTCAG GTTTCCTGGT TAGTTTTATG GTGGATGCCC GAGGTGGTGC TATGCGAGGA 
TGCAGACACA ACGGGCTCCG CATCATTATT CCGCCCCGGA AATGTACCGC CCCAACACGA 
GTCACCTGCC GACTGGTCAA ACGCCATAGA CTGGCGACAA TGCCTCCAAT GGTGGAAGGA 
GAGGGCCTGG CCAGTCGCCT GATTGAAGTT GGACCTTCTG GTGCTCAGTT CCTTGGGCCT 
GTGATCGTGG AGATCCCTCA TTTCGCTGCC CTGCGAGGAA AAGAGAGGGA ACTGGTGGTC 
CTGCGCAGTG AGAACGGGGA CAGCTGGAAA GAGCATTACT GTGAATACAC GGAGGAGGAG 
CTGAATGAAA TCCTGAACGG CATGGATGAA GTGCTGGATA GCCCGGAAGA CCTCGAAAAG 
AAGCGGATCT GCCGCATCAT CACCCGTGAC TTCCCACAGT ACTTTGCGGT GGTGTCGCGC 
ATCAAGCAGG ACAGCAACCT GATTGGCCCG GAGGGAGGCG TGCTGAGCAG CACCGTGGTG 
CCGCAGGTGC AGGCCGTGTT CCCCGAGGGG GCGCTAACCA AGCGGATCCG TGTTGGCCTA 
CAGGCTCAAC CTATGCACAG TGAGTTGGTT AAGAAGATTC TGGGCAACAA AGCTACCTTC 
AGCCCTATAG TTACTTTGGA ACCTAGAAGA AGAAAATTTC ACAAGCCAAT TACCATGACC 
ATTCCTGTCC CCAAAGCTTC AAGTGATGTC ATGTTGAATG GTTTTGGAGG AGATGCACCA 
ACCTTAAGAT TACTATGTAG CATCACAGGT GGAACCACCC CTGCTCAGTG GGAAGATATT 
ACAGGAACTA CGCCATTAAC ATTTGTCAAT GAATGTGTTT CCTTTACAAC CAATGTGTCT 
GCCAGGTTCT GGCTGATCGA TTGTCGACAA ATTCAGGAAT CTGTTACCTT TGCATCACAA 
GTGTATAGAG AAATTATCTG TGTACCATAT ATGGCCAAAT TTGTAGTGTT TGCCAAATCA 
CATGACCCCA TTGAAGCCAG GTTGAGGTGT TTCTGCATGA CTGATGATAA AGTGGATAAA 
ACCCTGGAAC AACAAGAAAA CTTTGCTGAG GTGGCCAGAA GCAGGGATGT GGAGGTCTTA 
GAAGGAAAAC CCATCTATGT TGATTGTTTT GGCAACCTGG TGCCATTAAC CAAGAGTGGC 
CAACATCATA TATTCAGTTT TTTTGCCTTC AAAGAAAACA GACTTCCTCT CTTTGTCAAG 
GTACGTGATA CGACTCAGGA ACCTTGCGGA CGACTATCAT TTATGAAAGA GCCAAAATCT 
ACAAGAGGCC TGGTGCACCA AGCTATTTGC AACTTAAACA TCACCCTGCC AATTTATACG 
AAGGAATCAG AGTCAGATCA AGAACAGGAG GAAGAGATCG ATATGACATC AGAAAAAAAT 
CAACAGGATG AGCAGGAACG GATTGAGGAA AGGCTGGCAT ATATTGCTGA TCACCTTGGC 
TTCAGCTGGA CAGAGTTGGC AAGAGAACTG GACTTCACTG AAGAGCAAAT TCATCAAATT 
CGAATTGAGA ACCCTAACTC CCTGCAGGAT CAGAGTCATG CGCTGTTGAA GTACTGGCTA 
GAAAGGGACG GGAGACATGC TACAGATACC AGTCTCGTTG AATGTCTCAC CAAGATCAAC 
CGAATGGATA TCGTGCATCT CATGGAGACC AGCACAGAAG CTCTCCAGGA GCGCATCGGT 
CATAGCTATG CAGAGATTGA GCAGACCATT ACACTGGATC ATAGTGAAGG GTTCTCAGTA 
CTCCAAGAGG AGCTTTGCAC TGCCCAGCAC AAGCAGAAAG AGGAGCATGC TGCTTCTAAA 
GAGGACGAGG CCTCTGACCA CCCTCCCATC GTCTCAGAGG AAGACATTTC TGTTGGTTAC 
TCCACTCTTC AGGATGGCAT CCCCAAAACT GAGGGTGATG GCTCTGCAAC AGAGCTCCTT 
CCCCAGACTC AAAAGGAGCA AGTTCAACAG GATTTCTCAG GGAAAATGCA AGACCTGCCT 
GAGGAGTCGG CTCTTGAACA GCAAGAATAC TTCGTGACAA CCCCAGGGAC AGAAATGTCA 
GAGACTCAGA AGGCCACGGG AACATCGGGC TCCCCCACCA AGACACCTGA GGAAATTGTC 
ACCCCTTCCG AGGAGGAGAG GCTGTATCTC CAAACCCCAA CATCGAGTGA GCGGGGAGGC 
TCTCCAGTTG TGCAGGAACC TGAGGAGCCC CCCGAACGCA GGGAGGAGAG TTCACCTCGG 
AAAACCAGCC TCGTGATCGT GGAATCGGCT GAGGACCAGC CTCAGGCCTT CGAGAGGCTT 
GATGAAGAGG CTGCTTTTCA AAAGGGAGAT GATATGCCTG ACATACCCCC AGAAACAGTC 
ACAGAAGAAG AATACATCGA TGAGCATGGA CACACCGTGG TAAAGAAGGT TACTAGGAAA 
ATCATTAGGC GGTTCATCTC CTCTGACGGC ACAGAGAAAG AGGAGATTAC GATGCAGGGA 
GCACCACAGG ATTCTATCAG CGTTGATGAA GGGGATGGTT ATTCCAAAGT CATAAAGCGC 
ATTGTATTGA AGAGTGACAC CGAGCAGTCA GAGGTAACTT TGTCTGAGCC CAGCATTCTG 
TCCAGTACCT CACAATTTCA GGCTGAACCA GTGGAAGGCC GTAGAGTCAG CAAAGTTGTT 
AAGACAACTG TGGTCCATGG TGAGAGGATG GAGAAACATC TGGGGGATTC TAGTTTAGCC 
ACTGATCTTC CTTCAGCCAA AGATGACTTT GAAGAGGCTT TGAGTTACAC AGGTAGCCAC 
ATGAAAGTCT ACTCCCCCAG TTTAGTAGAG AATGAAGTCC TGAAGGAGGA TGGATCAATA 
ATTAGAAGGA CGACAATGAG TAAAGCTAGA ACCCAGAAGA GGGCTGTGGT GAAGGACCAG 
CAGGGAAAAC GCATTTACTT GGAGCACCTG GAGGATGTCC CAGAAGCGCT AGACCAGGAC 
GACCTCCAGC ACGACCTCCA GCAACTCCTT CGGCATTTCT GCAAGGAGGA CTCGAAGCAA 
GAGGCCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849257.1
CDS	239..6250
Translation	MGNAALCPACHADKSVRAQGHLQELDRTPDYYGCNGEDTKELGPRSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSAVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHAMAKNGYTPLHIAAKKNQMQIASTLLSYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMTEVLDVSDEEGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSHTLSHASYLRDSAMIDDAVVIPSHQVTTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGFLVSFMVDARGGAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHYCEYTEEELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEIDMTSEKNQQDEQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGRHATDTSLVECLTKINRMDIVHLMETSTEALQERIGHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEHAASKEDEASDHPPIVSEEDISVGYSTLQDGIPKTEGDGSATELLPQTQKEQVQQDFSGKMQDLPEESALEQQEYFVTTPGTEMSETQKATGTSGSPTKTPEEIVTPSEEERLYLQTPTSSERGGSPVVQEPEEPPERREESSPRKTSLVIVESAEDQPQAFERLDEEAAFQKGDDMPDIPPETVTEEEYIDEHGHTVVKKVTRKIIRRFISSDGTEKEEITMQGAPQDSISVDEGDGYSKVIKRIVLKSDTEQSEVTLSEPSILSSTSQFQAEPVEGRRVSKVVKTTVVHGERMEKHLGDSSLATDLPSAKDDFEEALSYTGSHMKVYSPSLVENEVLKEDGSIIRRTTMSKARTQKRAVVKDQQGKRIYLEHLEDVPEALDQDDLQHDLQQLLRHFCKEDSKQEAQ

Target ORF information:

RefSeq Version	XM_024993489.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin 2 (ANK2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993489.1

ORF Insert Sequence:

ATGGGGAATG CAGCTCTCTG CCCGGCTTGC CATGCGGATA AGAGCGTAAG AGCACAGGGA 
CACTTGCAGG AATTGGACAG AACCCCAGAC TACTATGGCT GTAACGGCGA GGACACCAAA 
GAACTTGGGC CGCGGTCTGA CAGCAATGCA AGCTTCCTCC GTGCCGCCAG AGCAGGCAAC 
TTAGACAAAG TTGTGGAATA TCTGAAGGGG GGCATAGACA TCAATACCTG CAACCAGAAT 
GGACTCAACG CGCTTCACCT GGCTGCCAAG GAAGGCCACG TGGGGCTAGT TCAGGAGCTG 
CTGGGAAGAG GGTCTGCTGT GGACTCTGCC ACCAAGAAAG GAAATACTGC TCTTCACATT 
GCATCTTTGG CTGGACAAGC AGAAGTTGTC AAAGTTCTCG TCAAGGAAGG AGCCAATATT 
AATGCACAGT CTCAGAATGG CTTCACTCCT TTATACATGG CTGCCCAAGA GAATCACATT 
GATGTTGTAA AATATTTGCT GGAAAATGGA GCTAATCAGA GCACCGCTAC TGAGGATGGC 
TTTACGCCCC TGGCGGTGGC GCTCCAGCAG GGACACAACC AGGCAGTGGC CATCCTCCTG 
GAGAACGACA CCAAAGGGAA GGTGCGGCTG CCAGCCCTGC ATATTGCAGC CAGGAAAGAT 
GACACCAAAT CTGCCGCCCT CCTGCTTCAG AACGATCACA ACGCTGACGT ACAGTCCAAG 
ATGATGGTGA ATAGGACCAC CGAGAGTGGA TTCACCCCTT TGCACATCGC TGCCCACTAT 
GGAAACGTCA ACGTGGCAAC TCTCCTTCTA AACCGGGGAG CTGCTGTGGA CTTCACAGCC 
AGGAATGGAA TCACTCCTCT GCATGTGGCC TCCAAAAGGG GAAACACAAA CATGGTGAAG 
CTCTTACTGG ATCGTGGTGG TCAGATTGAT GCCAAAACTA GGGATGGGTT GACACCCCTT 
CATTGTGCTG CAAGAAGCGG TCATGACCAA GTGGTGGAGC TTCTGCTGGA GCGGGGCGCT 
CCCTTGCTGG CAAGGACGAA GAACGGGCTG TCTCCACTTC ACATGGCTGC ACAGGGGGAC 
CACGTGGAAT GTGTGAAGCA CCTGCTCCAG CACAAGGCGC CTGTTGATGA CGTCACTTTG 
GACTACTTGA CGGCCCTCCA CGTTGCCGCA CACTGCGGCC ACTACCGAGT AACCAAACTT 
CTTCTGGACA AGAGAGCCAA CCCGAACGCC AGAGCCCTGA ATGGTTTTAC TCCACTGCAC 
ATTGCCTGCA AGAAAAACCG CATCAAAGTC ATGGAACTGC TGGTGAAATA TGGGGCTTCA 
ATCCAAGCTA TAACAGAGTC TGGCCTCACA CCAATACATG TGGCTGCCTT CATGGGCCAC 
TTGAACATTG TCCTCCTTCT GCTGCAGAAC GGAGCCTCTC CAGATGTCAC TAACATTCGT 
GGGGAGACGG CCCTGCACAT GGCGGCCCGT GCAGGGCAGG TGGAAGTGGT CCGATGCCTC 
CTGAGGAACG GTGCCCTCGT CGATGCCAGA GCCAGGGAGG AACAGACGCC TTTGCATATT 
GCCTCCCGCC TGGGTAAGAC AGAAATTGTT CAGCTGCTTC TGCAACATAT GGCTCATCCA 
GATGCGGCCA CTACAAATGG GTACACGCCG TTGCATATCT CTGCAAGGGA AGGACAGGTG 
GACGTGGCCT CGGTCCTCCT GGAAGCGGGG GCAGCCCACT CCTTGGCTAC CAAGAAGGGT 
TTTACCCCCT TGCACGTAGC TGCCAAGTAT GGGAGCCTGG ATGTGGCAAA ACTTCTCTTG 
CAACGCCGTG CTGCCGCAGA TTCTGCGGGG AAGAATGGCC TTACCCCGCT CCATGTTGCT 
GCTCACTATG ACAACCAGAA GGTGGCGCTG CTGTTACTGG AGAAGGGTGC TTCCCCTCAC 
GCCATGGCCA AGAATGGCTA CACTCCTTTA CATATTGCTG CCAAGAAGAA TCAAATGCAG 
ATAGCTTCCA CACTCCTGAG CTACGGAGCA GAGACGAACA TCGTGACAAA GCAAGGAGTC 
ACTCCACTCC ATCTGGCCTC GCAGGAGGGA CACACGGATA TGGTCACCTT GCTTCTGGAT 
AAGGGAGCCA ATATCCACAT GTCAACCAAG AGTGGACTCA CATCTTTACA CCTTGCAGCC 
CAGGAAGATA AAGTGAATGT CGCTGATATT CTCACCAAGC ATGGGGCTGA CCAGGATGCG 
CACACAAAGC TTGGTTACAC TCCTTTAATT GTGGCCTGTC ACTATGGAAA CGTGAAAATG 
GTCAACTTCC TTCTGAAGCA GGGAGCAAAT GTCAATGCAA AAACCAAGAA TGGCTACACG 
CCTTTGCACC AAGCCGCCCA ACAGGGCCAC ACGCACATCA TCAATGTTTT GCTGCAGCAC 
GGGGCCAAGC CCAACGCTAC CACCGCGAAT GGCAACACTG CCTTGGCGAT TGCTAAGCGT 
CTGGGCTACA TCTCGGTGGT GGACACCCTG AAGGTTGTAA CGGAGGAGGT CACCACCACC 
ACCACGACTA TCACTGAAAA ACACAAGCTA AATGTTCCTG AGACCATGAC TGAGGTTCTT 
GATGTTTCTG ATGAAGAGGG TGATGACACA ATGACGGGTG ATGGGGGAGA GTACCTTAGA 
CCTGAGGACC TAAAGGAGCT GGGTGATGAC TCCCTCCCCA GCAGTCAGTT CCTGGACGGG 
ATGAATTACC TGCGCTACAG CTTGGAGGGG GGCCGCTCCG ACAGCCTTCG ATCCTTCAGT 
TCCGACAGGT CTCACACCCT GAGCCATGCC TCGTACCTGA GGGACAGTGC CATGATCGAC 
GACGCAGTTG TGATCCCCAG TCACCAGGTG ACAACTCTAG CCAAGGAGGC AGAAAGGAAT 
TCTTATCGTC TAAGCTGGGG CACTGAGAAC TTGGACAACG TGGCTCTTTC TTCCAGCCCT 
ATTCATTCAG GTTTCCTGGT TAGTTTTATG GTGGATGCCC GAGGTGGTGC TATGCGAGGA 
TGCAGACACA ACGGGCTCCG CATCATTATT CCGCCCCGGA AATGTACCGC CCCAACACGA 
GTCACCTGCC GACTGGTCAA ACGCCATAGA CTGGCGACAA TGCCTCCAAT GGTGGAAGGA 
GAGGGCCTGG CCAGTCGCCT GATTGAAGTT GGACCTTCTG GTGCTCAGTT CCTTGGGCCT 
GTGATCGTGG AGATCCCTCA TTTCGCTGCC CTGCGAGGAA AAGAGAGGGA ACTGGTGGTC 
CTGCGCAGTG AGAACGGGGA CAGCTGGAAA GAGCATTACT GTGAATACAC GGAGGAGGAG 
CTGAATGAAA TCCTGAACGG CATGGATGAA GTGCTGGATA GCCCGGAAGA CCTCGAAAAG 
AAGCGGATCT GCCGCATCAT CACCCGTGAC TTCCCACAGT ACTTTGCGGT GGTGTCGCGC 
ATCAAGCAGG ACAGCAACCT GATTGGCCCG GAGGGAGGCG TGCTGAGCAG CACCGTGGTG 
CCGCAGGTGC AGGCCGTGTT CCCCGAGGGG GCGCTAACCA AGCGGATCCG TGTTGGCCTA 
CAGGCTCAAC CTATGCACAG TGAGTTGGTT AAGAAGATTC TGGGCAACAA AGCTACCTTC 
AGCCCTATAG TTACTTTGGA ACCTAGAAGA AGAAAATTTC ACAAGCCAAT TACCATGACC 
ATTCCTGTCC CCAAAGCTTC AAGTGATGTC ATGTTGAATG GTTTTGGAGG AGATGCACCA 
ACCTTAAGAT TACTATGTAG CATCACAGGT GGAACCACCC CTGCTCAGTG GGAAGATATT 
ACAGGAACTA CGCCATTAAC ATTTGTCAAT GAATGTGTTT CCTTTACAAC CAATGTGTCT 
GCCAGGTTCT GGCTGATCGA TTGTCGACAA ATTCAGGAAT CTGTTACCTT TGCATCACAA 
GTGTATAGAG AAATTATCTG TGTACCATAT ATGGCCAAAT TTGTAGTGTT TGCCAAATCA 
CATGACCCCA TTGAAGCCAG GTTGAGGTGT TTCTGCATGA CTGATGATAA AGTGGATAAA 
ACCCTGGAAC AACAAGAAAA CTTTGCTGAG GTGGCCAGAA GCAGGGATGT GGAGGTCTTA 
GAAGGAAAAC CCATCTATGT TGATTGTTTT GGCAACCTGG TGCCATTAAC CAAGAGTGGC 
CAACATCATA TATTCAGTTT TTTTGCCTTC AAAGAAAACA GACTTCCTCT CTTTGTCAAG 
GTACGTGATA CGACTCAGGA ACCTTGCGGA CGACTATCAT TTATGAAAGA GCCAAAATCT 
ACAAGAGGCC TGGTGCACCA AGCTATTTGC AACTTAAACA TCACCCTGCC AATTTATACG 
AAGGAATCAG AGTCAGATCA AGAACAGGAG GAAGAGATCG ATATGACATC AGAAAAAAAT 
CAACAGGATG AGCAGGAACG GATTGAGGAA AGGCTGGCAT ATATTGCTGA TCACCTTGGC 
TTCAGCTGGA CAGAGTTGGC AAGAGAACTG GACTTCACTG AAGAGCAAAT TCATCAAATT 
CGAATTGAGA ACCCTAACTC CCTGCAGGAT CAGAGTCATG CGCTGTTGAA GTACTGGCTA 
GAAAGGGACG GGAGACATGC TACAGATACC AGTCTCGTTG AATGTCTCAC CAAGATCAAC 
CGAATGGATA TCGTGCATCT CATGGAGACC AGCACAGAAG CTCTCCAGGA GCGCATCGGT 
CATAGCTATG CAGAGATTGA GCAGACCATT ACACTGGATC ATAGTGAAGG GTTCTCAGTA 
CTCCAAGAGG AGCTTTGCAC TGCCCAGCAC AAGCAGAAAG AGGAGCATGC TGCTTCTAAA 
GAGGACGAGG CCTCTGACCA CCCTCCCATC GTCTCAGAGG AAGACATTTC TGTTGGTTAC 
TCCACTCTTC AGGATGGCAT CCCCAAAACT GAGGGTGATG GCTCTGCAAC AGAGCTCCTT 
CCCCAGACTC AAAAGGAGCA AGTTCAACAG GATTTCTCAG GGAAAATGCA AGACCTGCCT 
GAGGAGTCGG CTCTTGAACA GCAAGAATAC TTCGTGACAA CCCCAGGGAC AGAAATGTCA 
GAGACTCAGA AGGCCACGGG AACATCGGGC TCCCCCACCA AGACACCTGA GGAAATTGTC 
ACCCCTTCCG AGGAGGAGAG GCTGTATCTC CAAACCCCAA CATCGAGTGA GCGGGGAGGC 
TCTCCAGTTG TGCAGGAACC TGAGGAGCCC CCCGAACGCA GGGAGGAGAG TTCACCTCGG 
AAAACCAGCC TCGTGATCGT GGAATCGGCT GAGGACCAGC CTCAGGCCTT CGAGAGGCTT 
GATGAAGAGG CTGCTTTTCA AAAGGGAGAT GATATGCCTG ACATACCCCC AGAAACAGTC 
ACAGAAGAAG AATACATCGA TGAGCATGGA CACACCGTGG TAAAGAAGGT TACTAGGAAA 
ATCATTAGGC GGTTCATCTC CTCTGACGGC ACAGAGAAAG AGGAGATTAC GATGCAGGGA 
GCACCACAGG ATTCTATCAG CGTTGATGAA GGGGATGGTT ATTCCAAAGT CATAAAGCGC 
ATTGTATTGA AGAGTGACAC CGAGCAGTCA GAGGTAACTT TGTCTGAGCC CAGCATTCTG 
TCCAGTACCT CACAATTTCA GGCTGAACCA GTGGAAGGCC GTAGAGTCAG CAAAGTTGTT 
AAGACAACTG TGGTCCATGG TGAGAGGATG GAGAAACATC TGGGGGATTC TAGTTTAGCC 
ACTGATCTTC CTTCAGCCAA AGATGACTTT GAAGAGGCTT TGAGTTACAC AGGTAGCCAC 
ATGAAAGTCT ACTCCCCCAG TTTAGTAGAG AATGAAGTCC TGAAGGAGGA TGGATCAATA 
ATTAGAAGGA CGACAATGAG TAAAGCTAGA ACCCAGAAGA GGGCTGTGGT GAAGGACCAG 
CAGGGAAAAC GCATTTACTT GGAGCACCTG GAGGATGTCC CAGAAGCGCT AGACCAGGAC 
GACCTCCAGC ACGACCTCCA GCAACTCCTT CGGCATTTCT GCAAGGAGGA CTCGAAGCAA 
GAGGCCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK2 ( XM_024993485.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849253.1 Bos taurus ankyrin 2 (ANK2), transcript variant X1, mRNA.

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CloneID	OBa235477
Clone ID Related Accession (Same CDS sequence)	XM_024993485.1
Accession Version	XM_024993485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACG CTGCTGCTTC TATTAAAAAA GTTCGAGAGG CTGAACTGGA TGAAAAGGAG 
AAAAACCTGG AGAGAGAGAG GAAAAAACAA CGGAAAATCC CCAGGGAACG TATGGAACGG 
AAAAGAAAGT CTGACAGCAA TGCAAGCTTC CTCCGTGCCG CCAGAGCAGG CAACTTAGAC 
AAAGTTGTGG AATATCTGAA GGGGGGCATA GACATCAATA CCTGCAACCA GAATGGACTC 
AACGCGCTTC ACCTGGCTGC CAAGGAAGGC CACGTGGGGC TAGTTCAGGA GCTGCTGGGA 
AGAGGGTCTG CTGTGGACTC TGCCACCAAG AAAGGAAATA CTGCTCTTCA CATTGCATCT 
TTGGCTGGAC AAGCAGAAGT TGTCAAAGTT CTCGTCAAGG AAGGAGCCAA TATTAATGCA 
CAGTCTCAGA ATGGCTTCAC TCCTTTATAC ATGGCTGCCC AAGAGAATCA CATTGATGTT 
GTAAAATATT TGCTGGAAAA TGGAGCTAAT CAGAGCACCG CTACTGAGGA TGGCTTTACG 
CCCCTGGCGG TGGCGCTCCA GCAGGGACAC AACCAGGCAG TGGCCATCCT CCTGGAGAAC 
GACACCAAAG GGAAGGTGCG GCTGCCAGCC CTGCATATTG CAGCCAGGAA AGATGACACC 
AAATCTGCCG CCCTCCTGCT TCAGAACGAT CACAACGCTG ACGTACAGTC CAAGATGATG 
GTGAATAGGA CCACCGAGAG TGGATTCACC CCTTTGCACA TCGCTGCCCA CTATGGAAAC 
GTCAACGTGG CAACTCTCCT TCTAAACCGG GGAGCTGCTG TGGACTTCAC AGCCAGGAAT 
GGAATCACTC CTCTGCATGT GGCCTCCAAA AGGGGAAACA CAAACATGGT GAAGCTCTTA 
CTGGATCGTG GTGGTCAGAT TGATGCCAAA ACTAGGGATG GGTTGACACC CCTTCATTGT 
GCTGCAAGAA GCGGTCATGA CCAAGTGGTG GAGCTTCTGC TGGAGCGGGG CGCTCCCTTG 
CTGGCAAGGA CGAAGAACGG GCTGTCTCCA CTTCACATGG CTGCACAGGG GGACCACGTG 
GAATGTGTGA AGCACCTGCT CCAGCACAAG GCGCCTGTTG ATGACGTCAC TTTGGACTAC 
TTGACGGCCC TCCACGTTGC CGCACACTGC GGCCACTACC GAGTAACCAA ACTTCTTCTG 
GACAAGAGAG CCAACCCGAA CGCCAGAGCC CTGAATGGTT TTACTCCACT GCACATTGCC 
TGCAAGAAAA ACCGCATCAA AGTCATGGAA CTGCTGGTGA AATATGGGGC TTCAATCCAA 
GCTATAACAG AGTCTGGCCT CACACCAATA CATGTGGCTG CCTTCATGGG CCACTTGAAC 
ATTGTCCTCC TTCTGCTGCA GAACGGAGCC TCTCCAGATG TCACTAACAT TCGTGGGGAG 
ACGGCCCTGC ACATGGCGGC CCGTGCAGGG CAGGTGGAAG TGGTCCGATG CCTCCTGAGG 
AACGGTGCCC TCGTCGATGC CAGAGCCAGG GAGGAACAGA CGCCTTTGCA TATTGCCTCC 
CGCCTGGGTA AGACAGAAAT TGTTCAGCTG CTTCTGCAAC ATATGGCTCA TCCAGATGCG 
GCCACTACAA ATGGGTACAC GCCGTTGCAT ATCTCTGCAA GGGAAGGACA GGTGGACGTG 
GCCTCGGTCC TCCTGGAAGC GGGGGCAGCC CACTCCTTGG CTACCAAGAA GGGTTTTACC 
CCCTTGCACG TAGCTGCCAA GTATGGGAGC CTGGATGTGG CAAAACTTCT CTTGCAACGC 
CGTGCTGCCG CAGATTCTGC GGGGAAGAAT GGCCTTACCC CGCTCCATGT TGCTGCTCAC 
TATGACAACC AGAAGGTGGC GCTGCTGTTA CTGGAGAAGG GTGCTTCCCC TCACGCCATG 
GCCAAGAATG GCTACACTCC TTTACATATT GCTGCCAAGA AGAATCAAAT GCAGATAGCT 
TCCACACTCC TGAGCTACGG AGCAGAGACG AACATCGTGA CAAAGCAAGG AGTCACTCCA 
CTCCATCTGG CCTCGCAGGA GGGACACACG GATATGGTCA CCTTGCTTCT GGATAAGGGA 
GCCAATATCC ACATGTCAAC CAAGAGTGGA CTCACATCTT TACACCTTGC AGCCCAGGAA 
GATAAAGTGA ATGTCGCTGA TATTCTCACC AAGCATGGGG CTGACCAGGA TGCGCACACA 
AAGCTTGGTT ACACTCCTTT AATTGTGGCC TGTCACTATG GAAACGTGAA AATGGTCAAC 
TTCCTTCTGA AGCAGGGAGC AAATGTCAAT GCAAAAACCA AGAATGGCTA CACGCCTTTG 
CACCAAGCCG CCCAACAGGG CCACACGCAC ATCATCAATG TTTTGCTGCA GCACGGGGCC 
AAGCCCAACG CTACCACCGC GAATGGCAAC ACTGCCTTGG CGATTGCTAA GCGTCTGGGC 
TACATCTCGG TGGTGGACAC CCTGAAGGTT GTAACGGAGG AGGTCACCAC CACCACCACG 
ACTATCACTG AAAAACACAA GCTAAATGTT CCTGAGACCA TGACTGAGGT TCTTGATGTT 
TCTGATGAAG AGGCTTTTAA ACAGTTTGGT GACCACTTTA TTGATGGGGA AGTTCTTAGT 
GATTCAGGTG ATGACACAAT GACGGGTGAT GGGGGAGAGT ACCTTAGACC TGAGGACCTA 
AAGGAGCTGG GTGATGACTC CCTCCCCAGC AGTCAGTTCC TGGACGGGAT GAATTACCTG 
CGCTACAGCT TGGAGGGGGG CCGCTCCGAC AGCCTTCGAT CCTTCAGTTC CGACAGGTCT 
CACACCCTGA GCCATGCCTC GTACCTGAGG GACAGTGCCA TGATCGACGA CGCAGTTGTG 
ATCCCCAGTC ACCAGGTGAC AACTCTAGCC AAGGAGGCAG AAAGGAATTC TTATCGTCTA 
AGCTGGGGCA CTGAGAACTT GGACAACGTG GCTCTTTCTT CCAGCCCTAT TCATTCAGGC 
CGCACCTCTC CATGTCTTGA TCGTGACAAC AGCAGTTTCC TGGTTAGTTT TATGGTGGAT 
GCCCGAGGTG GTGCTATGCG AGGATGCAGA CACAACGGGC TCCGCATCAT TATTCCGCCC 
CGGAAATGTA CCGCCCCAAC ACGAGTCACC TGCCGACTGG TCAAACGCCA TAGACTGGCG 
ACAATGCCTC CAATGGTGGA AGGAGAGGGC CTGGCCAGTC GCCTGATTGA AGTTGGACCT 
TCTGGTGCTC AGTTCCTTGG TAAACTTCAC CTGCCAACGG CTCCTCCCCC ACTTAATGAG 
GGAGAAAGTT TGGTCAGCCG CATCCTTCAG CTGGGGCCTC CTGGAACCAA ATTCCTTGGG 
CCTGTGATCG TGGAGATCCC TCATTTCGCT GCCCTGCGAG GAAAAGAGAG GGAACTGGTG 
GTCCTGCGCA GTGAGAACGG GGACAGCTGG AAAGAGCATT ACTGTGAATA CACGGAGGAG 
GAGCTGAATG AAATCCTGAA CGGCATGGAT GAAGTGCTGG ATAGCCCGGA AGACCTCGAA 
AAGAAGCGGA TCTGCCGCAT CATCACCCGT GACTTCCCAC AGTACTTTGC GGTGGTGTCG 
CGCATCAAGC AGGACAGCAA CCTGATTGGC CCGGAGGGAG GCGTGCTGAG CAGCACCGTG 
GTGCCGCAGG TGCAGGCCGT GTTCCCCGAG GGGGCGCTAA CCAAGCGGAT CCGTGTTGGC 
CTACAGGCTC AACCTATGCA CAGTGAGTTG GTTAAGAAGA TTCTGGGCAA CAAAGCTACC 
TTCAGCCCTA TAGTTACTTT GGAACCTAGA AGAAGAAAAT TTCACAAGCC AATTACCATG 
ACCATTCCTG TCCCCAAAGC TTCAAGTGAT GTCATGTTGA ATGGTTTTGG AGGAGATGCA 
CCAACCTTAA GATTACTATG TAGCATCACA GGTGGAACCA CCCCTGCTCA GTGGGAAGAT 
ATTACAGGAA CTACGCCATT AACATTTGTC AATGAATGTG TTTCCTTTAC AACCAATGTG 
TCTGCCAGGT TCTGGCTGAT CGATTGTCGA CAAATTCAGG AATCTGTTAC CTTTGCATCA 
CAAGTGTATA GAGAAATTAT CTGTGTACCA TATATGGCCA AATTTGTAGT GTTTGCCAAA 
TCACATGACC CCATTGAAGC CAGGTTGAGG TGTTTCTGCA TGACTGATGA TAAAGTGGAT 
AAAACCCTGG AACAACAAGA AAACTTTGCT GAGGTGGCCA GAAGCAGGGA TGTGGAGGTC 
TTAGAAGGAA AACCCATCTA TGTTGATTGT TTTGGCAACC TGGTGCCATT AACCAAGAGT 
GGCCAACATC ATATATTCAG TTTTTTTGCC TTCAAAGAAA ACAGACTTCC TCTCTTTGTC 
AAGGTACGTG ATACGACTCA GGAACCTTGC GGACGACTAT CATTTATGAA AGAGCCAAAA 
TCTACAAGAG GCCTGGTGCA CCAAGCTATT TGCAACTTAA ACATCACCCT GCCAATTTAT 
ACGAAGGAAT CAGAGTCAGA TCAAGAACAG GAGGAAGAGA TCGATATGAC ATCAGAAAAA 
AATGATGAGA CAGAATCTAC AGAAACATCT GTCCTGAAAA GTCACCTGGT TAATGAAGTT 
CCTGTCCTGG CAAGTCCGGA CTTGCTCTCT GAAGTTTCTG AGATGAAACA AGATTTGATC 
AAAATGACCG CCATCTTGAC CACAGATGGA TCTGATAGGG CAGGTTCCAT CAAAGTGAAG 
GAGCTGGTGA AGGCTGCAGA GGAAGAGCCA GGGGAGCCTT TTGAAATTGT TGAAAGAGTT 
AAAGAGGACT TAGAGAAGGT GAACGAAATC CTGAGAAGCG GAACCTGCAC AGGAGATGAA 
GGCAGTGTGC CGAGGTCTCA GCCAGAGAGA GAGTTAGTGG AGGAGGAGTG GGTCATTGTC 
AGTGAGGAGG AGATAGAGGA GGCTAAGCAA AAAGCATCTT TAGAGATCGC CGAATACCCA 
TGTGTAGAAG TTAGACTAGA TAAAGAGACC AAAGTAGAAG TAGAGAAAGA CTCAACGGGG 
CTAGTGAACT ACCTTACTGA GGATCTGAAT TCCTATGTGC CTCCTCACGA AGAGCAGCCC 
CAGAAGGAGC AAGGTATGGT AGGGGAGAAG CCTGAGGCTC AGGGTCCCAG CAGGCGCTCA 
GAAAGCGAAG GCAAAGATGC ACCCTCAGAG GAGACACAGA GTACACAGAA ACAGCCCAAA 
CCAAGCCTGG GGATCAAGAA GCCGGTGAGA AGGAAATTAA AGGAGAAACA AAAGCAGAAG 
GAGGACAGTC CACAAGCTAG TGCAGAAAAA ACAGAACTTA AAAAAGGGAG TTCAGAAGAG 
TCATTAGATG AAGACACAAG TTCAGCCCCC GAGCCTCTTC CTGCTGTAAA AGCTACGTCA 
CCTTTGATAG AGGAAACGCC CATTGGTTCC ATAAAGGACA AGGTGAAGGC CCTTCAGAAA 
CGAGTGGAAG ATGAACAGAA GGGTCGAAGC AAGTTGCCTG TCAGAGTCAA AGGCAAAGAG 
GATGTGCCAA AAAAGGTAAC ACAAAGGACA CATTTGACTG CATCGCCCTC TCTGAAGTCA 
GAGAGGCATG CGCCAGCGTC CGCCTCCTCT AGAACAGAAA GACACACTTC TCTTTCCTCC 
TCTGCCAAAA CAGAAAGGCA CCCTCCGGTA TCACCATCGA GTAAGAACGA GAAACACTCG 
CCTGTGTCAC CCTCCGCGAA AGCTGAAAAG CAGTCCCCTG TGTCATCCTC AAGCAAAACT 
GAGAAACACT CACCCGTTTC ACCCTCGACC AAAACGGAAA GACACTCCCC TATGTCCTCT 
TCTACAAAAA CAGAAAGACA CCCTCCTGTT TCGCCTTCAG GTAAGACAGA TAAACGTCCC 
CCTGGATCTC CCTCTGGGAG ATCCGAGAAA CATCCGCCGG TATCTCCTGG CAGAACAGAG 
AAACGCTTGC CGGTGTCACC CGCTGGAAGG ATGGAAAAGC ATTCTCCTGT CTCAAGCTCC 
GGGAAAACTG AGAAGCACCT GCCCGTGTCA CCTTCTGGGA AAACAGACAA ACAACCACCT 
GTCTCCCCCA CCTCAAAAAC AGAAAGAATC GAGGAGACCA TGTCTGTTCG GGAGCTGATG 
AAAGCTTTCC AGTCAGGTCA GGACCCATCG AAACACAAGA CTGGACTTTT TGAGCACAAA 
TCTGCAAAAC CAAAACAGCC ACAAGAAAAG GGTAGAGGTC GGCTAGAAAA AGAAAAGGGG 
CCGATAGTAA CCCCAAGAGA AGCGCAGAAA ATGGAGAGTC AGACAATCAA ACGAGGCCAG 
AGGTTCCTGG TCACGGGATT CTCAGAACCC AGAAGAGCAG TCCGTGCTTC CTCCACAGGG 
TTTAAGAGAG AAGATGGGGC TGGAGAAAAG GAGAAATCTC TCAACCACAA AACACCCGAA 
CCTGTTCAGC CAGTGCCTGA AGAAGAAAGC CATAAAGATA GCGAAGTCCC CAAAGAAAAG 
CTGGCTGACG ACCAGGGAGA CATGGATCTC CAGATCAGCC CAGACCGGAA AACCTCCACT 
GACTTTTCTG ACGTCATTAA GCAAGAACTG GAAGACAATG ACAAATACCA GCAGTTCCGT 
ATGACTGAGG AGCCTGAGAA GGCACAGCTT CACTTAGACC AAGTGCTCAC TAGTCCTTTC 
AACACGACAT TTCCACTAGA TTACATGAAA GATGAGTTCC TCCCAGCCCT GTCTTTGCAG 
AGTGGCGCTT TACATGGCAG TTCTGAGAGC CTGAAGCCCG AAGGGGTGGC AGATTCTCCG 
TGTGGCAGCC TGATGGAAGG GACCCCTCAG ATTAGTTCAG AGGAAAGCTA TAAACATGAG 
GGCCTAGCCG AGACCCCCGA GACAAGCCCA GAGAGCCTTT CCTTCTCGCC AAAGAAAAGT 
GAGGAGCAGA TTGGGGAAAC ACATGAAACT ACCAAGTTAG AAACAGCACC AGAAAGTCAT 
TCCGAAAAAG GACATCCCTC AACCAAAGAT GTTGCTGATG GTTCTGAAGG GCAAGGTGCT 
GCAGTCACCG AGGCAGTAGA GTCCTCTGCT GAGCATCAGC TGACAGAGGC CACCCCAGAC 
CCTTCCAAAG ACATATGCCC CCCACAGGAA GGTGACAGCC TTGGTGGTCA AAGTGTATCA 
TTAGCAAAAG AAACATCCGA AGGACTGACT GAGGAAGTGA CCTGTGACGA AGGTCCACGT 
GCACACATTA GTCCATCCCA CGAAACACAA ACTGATACCG AAGCTCAGGA ATCCACAGCC 
ACCAAGCCCT CAGGGGAGGC AGAGGCCTCA CCGTTGTCCG AGGCTGCTGT AAAGGCAGGT 
TCAGGGACTG AATCAAAGCC CCAGGCAGCC ATTAGAAGTC CGCAAGGGTT AGAACTCGCA 
CTCCCTAGCC GCGAGAGTGA GGTTTTCAGC CCTGGGGCTG ATGAATCCTT CGCTGTGAGC 
CACAAAGACT CCCTGGAGGC CAGCCCTGTG CTGGAAGATA ACTCTTCGCA CAAAACTCCC 
GATTCTCTGG AACCAAGTCC TCTGAAGGAA TCCCCTTGCC GTGACTCTCT TGAAAGCAGC 
CCCGTTGAAC CAAAGATGAA GGCTGGAATT CTCCCAAGTC ACTTTCCTCT TCCCTCAGCT 
GTTGCCAAGA CCGAGCTCTT CACGGAAGTG GCCTCTGTGC GGTCCCGGCT GCTCCGAGAC 
CCCGACGGCA GTGCCGAGGA TGACAGCCTC GAGCAGACGT CACTCATGGA GAGCTCGGGG 
AAGAGTCCCC TTTCCCCAGA CACCCCCAGC TCCGAAGAAG TTAGCTACGA GGTCACGCCC 
AAAACCACAG ACACACCAAA ACCAGCTGTG ATTCCTGAAT GTGCGGAGGA GGACGACTCA 
GAAAATGGGG AGAAAAAGAG GTTCACACCG GAAGAGGAAA TGTTTAAAAT GGTAACCAAA 
GTCAAAACGT TTGATGAACT TGAACAAGAA GCAAAGCAGA AAAGGGACTA CAAAAGAGAA 
CCCAAGCAGG AAGAATCTTC TTCATCCTCT GATGCAGATG TCGACTGTTC AGCAGACACG 
GATGAACCAA AACACACAGA GAGTGTCGAG GATGAAAGTG ACGCTCCTGT GGTAGTAACA 
ACAGAGGGCA GGGAGGCTTC TTCCTCCTCA GAAAGTGAAC CTGAGTTGAC ACAGCTGAAG 
AAAGGTGCTG ACTCAGGTCT CTTGGCAGAG CCAGTTATTC GAGTGCAGCC TCCTTCCCCA 
CTTCCATCCA GCATAGACTC AAATTCCAGT CCTGAAGAAA TACAATTCCA GCCTATCGTT 
TCCAAACAGT ACACTTTTAA GATGAATGAA GATGCTCAGG AAGATTCAGA TAAATCAGAA 
GAAGAAAAAG ATTATAAATC TCGCTTACCC GAAGACAGTC ATGCTCTTCC CACTGATGGA 
CCAGATATGT CTTATGATGA TCTGAATGGA GATACTTATC AATCAAAACT TTGTGATGGC 
CTTGAGTATG AGATTGGGAG TCCTAGCGAC TCAGCTGCTC CTGTCTCTTC AGGTCTCCGC 
AGTTCCCCTG GTGACGATGT CAGTGAGCAG CTAGTTATCC ATAAGGAATC ACTAGCCCTC 
CAAGACACTG GTGAGAACGA CCTAGAAGAG CTTGATGTTC CTGAAGTCGA ATCTTCACAA 
GCAGATCCCC CCAGTGAAGG CTCTCCATCT TTCTCCTCTT TACCTCTTTG TCCAGCATCT 
GAAGCAAAAG AATTAGATGA AGACATGGTC TCTACAGCTT CTGCTACAGA AATGGAGGTC 
ACAAAACCTG ACCAAGCTTT TGAGAGCTTA CCAGAGGACT GTTCCATTCA AGATTCACTC 
ATTACTACTC AAACTGATAG ATCATCCTTG GATGTTCCAG TGTCAGACCC AACAGAGACT 
GAGGAAATCT ATGATCCTCA AATCATAAGC CCTTATGAAA ATGTTCCTTC TCAATCTTTT 
TTCTCTGGTG AAGAAAGCAA AACCCAAACG GATACAAGGC ACACAAGTTT TCATTCTTCT 
GAAGAGTATT CTGTTACCAT CACATCCTCT GTTGAAGATG TAGTAGAGAC AAGCTCCTCT 
GATAAAACTG TTTCAAGTAA AGAAGCTAAT TTTGAGGAGC AGGTCTTGGA AGTAGAATCC 
AAACAAGAGA GTACCTTGCA GGAAATACAG TCACATGGAG CCTCCTCATC TTTGGAGCCA 
CCTGTACCAA AAACATCAAC AGTTGTCAGT GAACAAATAA GCAAAGTCAT CATCACAAAA 
ACTGATGTGG ATTCTGATTC CTGGAGTGAA ATCCGTGAGG ATGACGAAGC CTTTGAGGCT 
CGAGTGAAAG AGGAAGAACA GAAGATATTT GGTTTGATGG TAGACAGACA GTCACAGGGT 
ACCACCCCTG ACACCACTCC TGCTAGGACC CCAACTGAAG AGGGGACCCC AACCAGTGAG 
CAAAATCCAT TTCTCTTTCA GGAAGGAAAA TTGTTTGAGA TGACCCGAAG TGGTGCCATT 
GATATGACCA AAAGGTCCTA TGCCGATGAA AGCTTTCACT TTTTCCAAAT TGGGCAAGAA 
TCCAGGGAAG AGGCTGTCTC ACAAGACATG AAAGAAGGAG GTACAGGTGC TGAGGCCCCA 
CAGGTGGAGA CATCAGCTGA GTCATTAACA CTCTCAGAAT CAAAAGAAAC AGTGGATGAT 
GAAGCAGAGT TACTCCCAGA TGACCTGAGT GAGGAGGTAG AAGAAATACA CACTTCAGAT 
ACCCCACTTA GCTCCCAAAT AGACATTTCA GCCTCCACAG AAACATCCAT GAAAGAGGCT 
ACTTCTACAG GGCTTGAGGA CCTACCCACC AGGCAAATGG GTGATTCTCT TCCTCCATCC 
AGCGAGAAGC AGACCACTTG CCCTGACTTC CCTGAACAAG TTGTACAAGT TCAGTTAGAT 
TTTTCCACAG TCACTAGGTC TGTATATTCA GATCGGGGTG ATGATTCTCC CGATTCCTCC 
CCAGAGGAAC AGAAATCAGT CATTGAAATC CCTACTGCAA CCATGGAGAA TGTGCCTTCT 
ACTGAGAGCA AATCCAAAAT CCCTGTAAGG ACTATCCCCA CTTCAACCTC AATACCTCCA 
TCGGTGGAAT ATGAGAGTTC CCTTTCTGAA GATTTTCTGC CCAGCCTGGA TGAGGAAAGT 
AAAGAAGATG AAGCAAAACC AAAGTCTAAA ATCCCCGTGA AAGCACCTGC CCAAAGAGTG 
GAGCAGCAGC CTTCACATCT AGACTCACCT CTAAAGAAGA CAGGTCGTCC CCAGGGACAG 
GACTTGACAA GCAGTGCACC AGATGATAGA AGCAAATCTG AATCTGATGC CAGTTTGGAC 
GTGAAGACCA AATGTCCAGT AGAAACTAGA AGTTACACTG AGGCAGAAAT AGAAAGCACA 
GGTGAGGAAG AGGAGCTTGA GTTAGAATCA GAGGAAGGGG CCACAAGACC AAAGATATTT 
GCATCCCGAT TGCCAGTAAA GAGCAAAAGC ACCGCATCTT CCTGCAGGGG GCCCACAAGC 
CCCACAAAAG ACAATAAGGA GCATTTCTTT GATCTTTACA GGAACTCCAT AGAATTCTTT 
GAGGAGATTA GTGATGAAGC TTCCAAGTTA GTGGATAGAC TTACACAGTC AGAAAGGGAG 
CAAGATTTAG TTTCAGATGA TGAAAGTAGT AGTGCCCTGG AAGTTTCAGT AATTGAAAAT 
CTGCCACCTG TTGAGACCGA GAACTCAATT CCTGAGGACC TCTTTGACAC AAGGCCCATT 
TGGGATGAGT CCATTGAGAC TCTGATCGAA CGCATCCCTG ATGAAAATGG CCATGACCAT 
GCTGAAGATC AACAGGATGA GCAGGAACGG ATTGAGGAAA GGCTGGCATA TATTGCTGAT 
CACCTTGGCT TCAGCTGGAC AGAGTTGGCA AGAGAACTGG ACTTCACTGA AGAGCAAATT 
CATCAAATTC GAATTGAGAA CCCTAACTCC CTGCAGGATC AGAGTCATGC GCTGTTGAAG 
TACTGGCTAG AAAGGGACGG GAGACATGCT ACAGATACCA GTCTCGTTGA ATGTCTCACC 
AAGATCAACC GAATGGATAT CGTGCATCTC ATGGAGACCA GCACAGAAGC TCTCCAGGAG 
CGCATCGGTC ATAGCTATGC AGAGATTGAG CAGACCATTA CACTGGATCA TAGTGAAGGG 
TTCTCAGTAC TCCAAGAGGA GCTTTGCACT GCCCAGCACA AGCAGAAAGA GGAGCATGCT 
GCTTCTAAAG AGGACGAGGC CTCTGACCAC CCTCCCATCG TCTCAGAGGA AGACATTTCT 
GTTGGTTACT CCACTCTTCA GGATGGCATC CCCAAAACTG AGGGTGATGG CTCTGCAACA 
GAGCTCCTTC CCCAGACTCA AAAGGAGCAA GTTCAACAGG ATTTCTCAGG GAAAATGCAA 
GACCTGCCTG AGGAGTCGGC TCTTGAACAG CAAGAATACT TCGTGACAAC CCCAGGGACA 
GAAATGTCAG AGACTCAGAA GGCCACGGGA ACATCGGGCT CCCCCACCAA GACACCTGAG 
GAAATTGTCA CCCCTTCCGA GGAGGAGAGG CTGTATCTCC AAACCCCAAC ATCGAGTGAG 
CGGGGAGGCT CTCCAGTTGT GCAGGAACCT GAGGAGCCCC CCGAACGCAG GGAGGAGAGT 
TCACCTCGGA AAACCAGCCT CGTGATCGTG GAATCGGCTG AGGACCAGCC TCAGGCCTTC 
GAGAGGCTTG ATGAAGAGGC TGCTTTTCAA AAGGAGCTAA CAGAGGAATT AGGTGAGCTG 
GAGGCCAGCT CAGATGAGGA GGCGATGGTA ACAACCAGGG TTGTCCGCCG ACGATTGATT 
ATTCAGGGAG ATGATATGCC TGACATACCC CCAGAAACAG TCACAGAAGA AGAATACATC 
GATGAGCATG GACACACCGT GGTAAAGAAG GTTACTAGGA AAATCATTAG GCGGTTCATC 
TCCTCTGACG GCACAGAGAA AGAGGAGATT ACGATGCAGG GAGCACCACA GGATTCTATC 
AGCGTTGATG AAGGGGATGG TTATTCCAAA GTCATAAAGC GCATTGTATT GAAGAGTGAC 
ACCGAGCAGT CAGAGGTAAC TTTGTCTGAG CCCAGCATTC TGTCCAGTAC CTCACAATTT 
CAGGCTGAAC CAGTGGAAGG CCGTAGAGTC AGCAAAGTTG TTAAGACAAC TGTGGTCCAT 
GGTGAGAGGA TGGAGAAACA TCTGGGGGAT TCTAGTTTAG CCACTGATCT TCCTTCAGCC 
AAAGATGACT TTGAAGAGGC TTTGAGTTAC ACAGGTAGCC ACATGAAAGT CTACTCCCCC 
AGTTTAGTAG AGAATGAAGT CCTGAAGGAG GATGGATCAA TAATTAGAAG GACGACAATG 
AGTAAAGCTA GAACCCAGAA GAGGGCTGTG GTGAAGGACC AGCAGGGAAA ACGCATTTAC 
TTGGAGCACC TGGAGGATGT CCCAGAAGCG CTAGACCAGG ACGACCTCCA GCACGACCTC 
CAGCAACTCC TTCGGCATTT CTGCAAGGAG GACTCGAAGC AAGAGGCCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849253.1
CDS	445..12978
Translation	MAHAAASIKKVREAELDEKEKNLERERKKQRKIPRERMERKRKSDSNASFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSAVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHAMAKNGYTPLHIAAKKNQMQIASTLLSYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMTEVLDVSDEEAFKQFGDHFIDGEVLSDSGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSHTLSHASYLRDSAMIDDAVVIPSHQVTTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGRTSPCLDRDNSSFLVSFMVDARGGAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGKLHLPTAPPPLNEGESLVSRILQLGPPGTKFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHYCEYTEEELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEIDMTSEKNDETESTETSVLKSHLVNEVPVLASPDLLSEVSEMKQDLIKMTAILTTDGSDRAGSIKVKELVKAAEEEPGEPFEIVERVKEDLEKVNEILRSGTCTGDEGSVPRSQPERELVEEEWVIVSEEEIEEAKQKASLEIAEYPCVEVRLDKETKVEVEKDSTGLVNYLTEDLNSYVPPHEEQPQKEQGMVGEKPEAQGPSRRSESEGKDAPSEETQSTQKQPKPSLGIKKPVRRKLKEKQKQKEDSPQASAEKTELKKGSSEESLDEDTSSAPEPLPAVKATSPLIEETPIGSIKDKVKALQKRVEDEQKGRSKLPVRVKGKEDVPKKVTQRTHLTASPSLKSERHAPASASSRTERHTSLSSSAKTERHPPVSPSSKNEKHSPVSPSAKAEKQSPVSSSSKTEKHSPVSPSTKTERHSPMSSSTKTERHPPVSPSGKTDKRPPGSPSGRSEKHPPVSPGRTEKRLPVSPAGRMEKHSPVSSSGKTEKHLPVSPSGKTDKQPPVSPTSKTERIEETMSVRELMKAFQSGQDPSKHKTGLFEHKSAKPKQPQEKGRGRLEKEKGPIVTPREAQKMESQTIKRGQRFLVTGFSEPRRAVRASSTGFKREDGAGEKEKSLNHKTPEPVQPVPEEESHKDSEVPKEKLADDQGDMDLQISPDRKTSTDFSDVIKQELEDNDKYQQFRMTEEPEKAQLHLDQVLTSPFNTTFPLDYMKDEFLPALSLQSGALHGSSESLKPEGVADSPCGSLMEGTPQISSEESYKHEGLAETPETSPESLSFSPKKSEEQIGETHETTKLETAPESHSEKGHPSTKDVADGSEGQGAAVTEAVESSAEHQLTEATPDPSKDICPPQEGDSLGGQSVSLAKETSEGLTEEVTCDEGPRAHISPSHETQTDTEAQESTATKPSGEAEASPLSEAAVKAGSGTESKPQAAIRSPQGLELALPSRESEVFSPGADESFAVSHKDSLEASPVLEDNSSHKTPDSLEPSPLKESPCRDSLESSPVEPKMKAGILPSHFPLPSAVAKTELFTEVASVRSRLLRDPDGSAEDDSLEQTSLMESSGKSPLSPDTPSSEEVSYEVTPKTTDTPKPAVIPECAEEDDSENGEKKRFTPEEEMFKMVTKVKTFDELEQEAKQKRDYKREPKQEESSSSSDADVDCSADTDEPKHTESVEDESDAPVVVTTEGREASSSSESEPELTQLKKGADSGLLAEPVIRVQPPSPLPSSIDSNSSPEEIQFQPIVSKQYTFKMNEDAQEDSDKSEEEKDYKSRLPEDSHALPTDGPDMSYDDLNGDTYQSKLCDGLEYEIGSPSDSAAPVSSGLRSSPGDDVSEQLVIHKESLALQDTGENDLEELDVPEVESSQADPPSEGSPSFSSLPLCPASEAKELDEDMVSTASATEMEVTKPDQAFESLPEDCSIQDSLITTQTDRSSLDVPVSDPTETEEIYDPQIISPYENVPSQSFFSGEESKTQTDTRHTSFHSSEEYSVTITSSVEDVVETSSSDKTVSSKEANFEEQVLEVESKQESTLQEIQSHGASSSLEPPVPKTSTVVSEQISKVIITKTDVDSDSWSEIREDDEAFEARVKEEEQKIFGLMVDRQSQGTTPDTTPARTPTEEGTPTSEQNPFLFQEGKLFEMTRSGAIDMTKRSYADESFHFFQIGQESREEAVSQDMKEGGTGAEAPQVETSAESLTLSESKETVDDEAELLPDDLSEEVEEIHTSDTPLSSQIDISASTETSMKEATSTGLEDLPTRQMGDSLPPSSEKQTTCPDFPEQVVQVQLDFSTVTRSVYSDRGDDSPDSSPEEQKSVIEIPTATMENVPSTESKSKIPVRTIPTSTSIPPSVEYESSLSEDFLPSLDEESKEDEAKPKSKIPVKAPAQRVEQQPSHLDSPLKKTGRPQGQDLTSSAPDDRSKSESDASLDVKTKCPVETRSYTEAEIESTGEEEELELESEEGATRPKIFASRLPVKSKSTASSCRGPTSPTKDNKEHFFDLYRNSIEFFEEISDEASKLVDRLTQSEREQDLVSDDESSSALEVSVIENLPPVETENSIPEDLFDTRPIWDESIETLIERIPDENGHDHAEDQQDEQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGRHATDTSLVECLTKINRMDIVHLMETSTEALQERIGHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEHAASKEDEASDHPPIVSEEDISVGYSTLQDGIPKTEGDGSATELLPQTQKEQVQQDFSGKMQDLPEESALEQQEYFVTTPGTEMSETQKATGTSGSPTKTPEEIVTPSEEERLYLQTPTSSERGGSPVVQEPEEPPERREESSPRKTSLVIVESAEDQPQAFERLDEEAAFQKELTEELGELEASSDEEAMVTTRVVRRRLIIQGDDMPDIPPETVTEEEYIDEHGHTVVKKVTRKIIRRFISSDGTEKEEITMQGAPQDSISVDEGDGYSKVIKRIVLKSDTEQSEVTLSEPSILSSTSQFQAEPVEGRRVSKVVKTTVVHGERMEKHLGDSSLATDLPSAKDDFEEALSYTGSHMKVYSPSLVENEVLKEDGSIIRRTTMSKARTQKRAVVKDQQGKRIYLEHLEDVPEALDQDDLQHDLQQLLRHFCKEDSKQEAQ

Target ORF information:

RefSeq Version	XM_024993485.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin 2 (ANK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993485.1

ORF Insert Sequence:

ATGGCTCACG CTGCTGCTTC TATTAAAAAA GTTCGAGAGG CTGAACTGGA TGAAAAGGAG 
AAAAACCTGG AGAGAGAGAG GAAAAAACAA CGGAAAATCC CCAGGGAACG TATGGAACGG 
AAAAGAAAGT CTGACAGCAA TGCAAGCTTC CTCCGTGCCG CCAGAGCAGG CAACTTAGAC 
AAAGTTGTGG AATATCTGAA GGGGGGCATA GACATCAATA CCTGCAACCA GAATGGACTC 
AACGCGCTTC ACCTGGCTGC CAAGGAAGGC CACGTGGGGC TAGTTCAGGA GCTGCTGGGA 
AGAGGGTCTG CTGTGGACTC TGCCACCAAG AAAGGAAATA CTGCTCTTCA CATTGCATCT 
TTGGCTGGAC AAGCAGAAGT TGTCAAAGTT CTCGTCAAGG AAGGAGCCAA TATTAATGCA 
CAGTCTCAGA ATGGCTTCAC TCCTTTATAC ATGGCTGCCC AAGAGAATCA CATTGATGTT 
GTAAAATATT TGCTGGAAAA TGGAGCTAAT CAGAGCACCG CTACTGAGGA TGGCTTTACG 
CCCCTGGCGG TGGCGCTCCA GCAGGGACAC AACCAGGCAG TGGCCATCCT CCTGGAGAAC 
GACACCAAAG GGAAGGTGCG GCTGCCAGCC CTGCATATTG CAGCCAGGAA AGATGACACC 
AAATCTGCCG CCCTCCTGCT TCAGAACGAT CACAACGCTG ACGTACAGTC CAAGATGATG 
GTGAATAGGA CCACCGAGAG TGGATTCACC CCTTTGCACA TCGCTGCCCA CTATGGAAAC 
GTCAACGTGG CAACTCTCCT TCTAAACCGG GGAGCTGCTG TGGACTTCAC AGCCAGGAAT 
GGAATCACTC CTCTGCATGT GGCCTCCAAA AGGGGAAACA CAAACATGGT GAAGCTCTTA 
CTGGATCGTG GTGGTCAGAT TGATGCCAAA ACTAGGGATG GGTTGACACC CCTTCATTGT 
GCTGCAAGAA GCGGTCATGA CCAAGTGGTG GAGCTTCTGC TGGAGCGGGG CGCTCCCTTG 
CTGGCAAGGA CGAAGAACGG GCTGTCTCCA CTTCACATGG CTGCACAGGG GGACCACGTG 
GAATGTGTGA AGCACCTGCT CCAGCACAAG GCGCCTGTTG ATGACGTCAC TTTGGACTAC 
TTGACGGCCC TCCACGTTGC CGCACACTGC GGCCACTACC GAGTAACCAA ACTTCTTCTG 
GACAAGAGAG CCAACCCGAA CGCCAGAGCC CTGAATGGTT TTACTCCACT GCACATTGCC 
TGCAAGAAAA ACCGCATCAA AGTCATGGAA CTGCTGGTGA AATATGGGGC TTCAATCCAA 
GCTATAACAG AGTCTGGCCT CACACCAATA CATGTGGCTG CCTTCATGGG CCACTTGAAC 
ATTGTCCTCC TTCTGCTGCA GAACGGAGCC TCTCCAGATG TCACTAACAT TCGTGGGGAG 
ACGGCCCTGC ACATGGCGGC CCGTGCAGGG CAGGTGGAAG TGGTCCGATG CCTCCTGAGG 
AACGGTGCCC TCGTCGATGC CAGAGCCAGG GAGGAACAGA CGCCTTTGCA TATTGCCTCC 
CGCCTGGGTA AGACAGAAAT TGTTCAGCTG CTTCTGCAAC ATATGGCTCA TCCAGATGCG 
GCCACTACAA ATGGGTACAC GCCGTTGCAT ATCTCTGCAA GGGAAGGACA GGTGGACGTG 
GCCTCGGTCC TCCTGGAAGC GGGGGCAGCC CACTCCTTGG CTACCAAGAA GGGTTTTACC 
CCCTTGCACG TAGCTGCCAA GTATGGGAGC CTGGATGTGG CAAAACTTCT CTTGCAACGC 
CGTGCTGCCG CAGATTCTGC GGGGAAGAAT GGCCTTACCC CGCTCCATGT TGCTGCTCAC 
TATGACAACC AGAAGGTGGC GCTGCTGTTA CTGGAGAAGG GTGCTTCCCC TCACGCCATG 
GCCAAGAATG GCTACACTCC TTTACATATT GCTGCCAAGA AGAATCAAAT GCAGATAGCT 
TCCACACTCC TGAGCTACGG AGCAGAGACG AACATCGTGA CAAAGCAAGG AGTCACTCCA 
CTCCATCTGG CCTCGCAGGA GGGACACACG GATATGGTCA CCTTGCTTCT GGATAAGGGA 
GCCAATATCC ACATGTCAAC CAAGAGTGGA CTCACATCTT TACACCTTGC AGCCCAGGAA 
GATAAAGTGA ATGTCGCTGA TATTCTCACC AAGCATGGGG CTGACCAGGA TGCGCACACA 
AAGCTTGGTT ACACTCCTTT AATTGTGGCC TGTCACTATG GAAACGTGAA AATGGTCAAC 
TTCCTTCTGA AGCAGGGAGC AAATGTCAAT GCAAAAACCA AGAATGGCTA CACGCCTTTG 
CACCAAGCCG CCCAACAGGG CCACACGCAC ATCATCAATG TTTTGCTGCA GCACGGGGCC 
AAGCCCAACG CTACCACCGC GAATGGCAAC ACTGCCTTGG CGATTGCTAA GCGTCTGGGC 
TACATCTCGG TGGTGGACAC CCTGAAGGTT GTAACGGAGG AGGTCACCAC CACCACCACG 
ACTATCACTG AAAAACACAA GCTAAATGTT CCTGAGACCA TGACTGAGGT TCTTGATGTT 
TCTGATGAAG AGGCTTTTAA ACAGTTTGGT GACCACTTTA TTGATGGGGA AGTTCTTAGT 
GATTCAGGTG ATGACACAAT GACGGGTGAT GGGGGAGAGT ACCTTAGACC TGAGGACCTA 
AAGGAGCTGG GTGATGACTC CCTCCCCAGC AGTCAGTTCC TGGACGGGAT GAATTACCTG 
CGCTACAGCT TGGAGGGGGG CCGCTCCGAC AGCCTTCGAT CCTTCAGTTC CGACAGGTCT 
CACACCCTGA GCCATGCCTC GTACCTGAGG GACAGTGCCA TGATCGACGA CGCAGTTGTG 
ATCCCCAGTC ACCAGGTGAC AACTCTAGCC AAGGAGGCAG AAAGGAATTC TTATCGTCTA 
AGCTGGGGCA CTGAGAACTT GGACAACGTG GCTCTTTCTT CCAGCCCTAT TCATTCAGGC 
CGCACCTCTC CATGTCTTGA TCGTGACAAC AGCAGTTTCC TGGTTAGTTT TATGGTGGAT 
GCCCGAGGTG GTGCTATGCG AGGATGCAGA CACAACGGGC TCCGCATCAT TATTCCGCCC 
CGGAAATGTA CCGCCCCAAC ACGAGTCACC TGCCGACTGG TCAAACGCCA TAGACTGGCG 
ACAATGCCTC CAATGGTGGA AGGAGAGGGC CTGGCCAGTC GCCTGATTGA AGTTGGACCT 
TCTGGTGCTC AGTTCCTTGG TAAACTTCAC CTGCCAACGG CTCCTCCCCC ACTTAATGAG 
GGAGAAAGTT TGGTCAGCCG CATCCTTCAG CTGGGGCCTC CTGGAACCAA ATTCCTTGGG 
CCTGTGATCG TGGAGATCCC TCATTTCGCT GCCCTGCGAG GAAAAGAGAG GGAACTGGTG 
GTCCTGCGCA GTGAGAACGG GGACAGCTGG AAAGAGCATT ACTGTGAATA CACGGAGGAG 
GAGCTGAATG AAATCCTGAA CGGCATGGAT GAAGTGCTGG ATAGCCCGGA AGACCTCGAA 
AAGAAGCGGA TCTGCCGCAT CATCACCCGT GACTTCCCAC AGTACTTTGC GGTGGTGTCG 
CGCATCAAGC AGGACAGCAA CCTGATTGGC CCGGAGGGAG GCGTGCTGAG CAGCACCGTG 
GTGCCGCAGG TGCAGGCCGT GTTCCCCGAG GGGGCGCTAA CCAAGCGGAT CCGTGTTGGC 
CTACAGGCTC AACCTATGCA CAGTGAGTTG GTTAAGAAGA TTCTGGGCAA CAAAGCTACC 
TTCAGCCCTA TAGTTACTTT GGAACCTAGA AGAAGAAAAT TTCACAAGCC AATTACCATG 
ACCATTCCTG TCCCCAAAGC TTCAAGTGAT GTCATGTTGA ATGGTTTTGG AGGAGATGCA 
CCAACCTTAA GATTACTATG TAGCATCACA GGTGGAACCA CCCCTGCTCA GTGGGAAGAT 
ATTACAGGAA CTACGCCATT AACATTTGTC AATGAATGTG TTTCCTTTAC AACCAATGTG 
TCTGCCAGGT TCTGGCTGAT CGATTGTCGA CAAATTCAGG AATCTGTTAC CTTTGCATCA 
CAAGTGTATA GAGAAATTAT CTGTGTACCA TATATGGCCA AATTTGTAGT GTTTGCCAAA 
TCACATGACC CCATTGAAGC CAGGTTGAGG TGTTTCTGCA TGACTGATGA TAAAGTGGAT 
AAAACCCTGG AACAACAAGA AAACTTTGCT GAGGTGGCCA GAAGCAGGGA TGTGGAGGTC 
TTAGAAGGAA AACCCATCTA TGTTGATTGT TTTGGCAACC TGGTGCCATT AACCAAGAGT 
GGCCAACATC ATATATTCAG TTTTTTTGCC TTCAAAGAAA ACAGACTTCC TCTCTTTGTC 
AAGGTACGTG ATACGACTCA GGAACCTTGC GGACGACTAT CATTTATGAA AGAGCCAAAA 
TCTACAAGAG GCCTGGTGCA CCAAGCTATT TGCAACTTAA ACATCACCCT GCCAATTTAT 
ACGAAGGAAT CAGAGTCAGA TCAAGAACAG GAGGAAGAGA TCGATATGAC ATCAGAAAAA 
AATGATGAGA CAGAATCTAC AGAAACATCT GTCCTGAAAA GTCACCTGGT TAATGAAGTT 
CCTGTCCTGG CAAGTCCGGA CTTGCTCTCT GAAGTTTCTG AGATGAAACA AGATTTGATC 
AAAATGACCG CCATCTTGAC CACAGATGGA TCTGATAGGG CAGGTTCCAT CAAAGTGAAG 
GAGCTGGTGA AGGCTGCAGA GGAAGAGCCA GGGGAGCCTT TTGAAATTGT TGAAAGAGTT 
AAAGAGGACT TAGAGAAGGT GAACGAAATC CTGAGAAGCG GAACCTGCAC AGGAGATGAA 
GGCAGTGTGC CGAGGTCTCA GCCAGAGAGA GAGTTAGTGG AGGAGGAGTG GGTCATTGTC 
AGTGAGGAGG AGATAGAGGA GGCTAAGCAA AAAGCATCTT TAGAGATCGC CGAATACCCA 
TGTGTAGAAG TTAGACTAGA TAAAGAGACC AAAGTAGAAG TAGAGAAAGA CTCAACGGGG 
CTAGTGAACT ACCTTACTGA GGATCTGAAT TCCTATGTGC CTCCTCACGA AGAGCAGCCC 
CAGAAGGAGC AAGGTATGGT AGGGGAGAAG CCTGAGGCTC AGGGTCCCAG CAGGCGCTCA 
GAAAGCGAAG GCAAAGATGC ACCCTCAGAG GAGACACAGA GTACACAGAA ACAGCCCAAA 
CCAAGCCTGG GGATCAAGAA GCCGGTGAGA AGGAAATTAA AGGAGAAACA AAAGCAGAAG 
GAGGACAGTC CACAAGCTAG TGCAGAAAAA ACAGAACTTA AAAAAGGGAG TTCAGAAGAG 
TCATTAGATG AAGACACAAG TTCAGCCCCC GAGCCTCTTC CTGCTGTAAA AGCTACGTCA 
CCTTTGATAG AGGAAACGCC CATTGGTTCC ATAAAGGACA AGGTGAAGGC CCTTCAGAAA 
CGAGTGGAAG ATGAACAGAA GGGTCGAAGC AAGTTGCCTG TCAGAGTCAA AGGCAAAGAG 
GATGTGCCAA AAAAGGTAAC ACAAAGGACA CATTTGACTG CATCGCCCTC TCTGAAGTCA 
GAGAGGCATG CGCCAGCGTC CGCCTCCTCT AGAACAGAAA GACACACTTC TCTTTCCTCC 
TCTGCCAAAA CAGAAAGGCA CCCTCCGGTA TCACCATCGA GTAAGAACGA GAAACACTCG 
CCTGTGTCAC CCTCCGCGAA AGCTGAAAAG CAGTCCCCTG TGTCATCCTC AAGCAAAACT 
GAGAAACACT CACCCGTTTC ACCCTCGACC AAAACGGAAA GACACTCCCC TATGTCCTCT 
TCTACAAAAA CAGAAAGACA CCCTCCTGTT TCGCCTTCAG GTAAGACAGA TAAACGTCCC 
CCTGGATCTC CCTCTGGGAG ATCCGAGAAA CATCCGCCGG TATCTCCTGG CAGAACAGAG 
AAACGCTTGC CGGTGTCACC CGCTGGAAGG ATGGAAAAGC ATTCTCCTGT CTCAAGCTCC 
GGGAAAACTG AGAAGCACCT GCCCGTGTCA CCTTCTGGGA AAACAGACAA ACAACCACCT 
GTCTCCCCCA CCTCAAAAAC AGAAAGAATC GAGGAGACCA TGTCTGTTCG GGAGCTGATG 
AAAGCTTTCC AGTCAGGTCA GGACCCATCG AAACACAAGA CTGGACTTTT TGAGCACAAA 
TCTGCAAAAC CAAAACAGCC ACAAGAAAAG GGTAGAGGTC GGCTAGAAAA AGAAAAGGGG 
CCGATAGTAA CCCCAAGAGA AGCGCAGAAA ATGGAGAGTC AGACAATCAA ACGAGGCCAG 
AGGTTCCTGG TCACGGGATT CTCAGAACCC AGAAGAGCAG TCCGTGCTTC CTCCACAGGG 
TTTAAGAGAG AAGATGGGGC TGGAGAAAAG GAGAAATCTC TCAACCACAA AACACCCGAA 
CCTGTTCAGC CAGTGCCTGA AGAAGAAAGC CATAAAGATA GCGAAGTCCC CAAAGAAAAG 
CTGGCTGACG ACCAGGGAGA CATGGATCTC CAGATCAGCC CAGACCGGAA AACCTCCACT 
GACTTTTCTG ACGTCATTAA GCAAGAACTG GAAGACAATG ACAAATACCA GCAGTTCCGT 
ATGACTGAGG AGCCTGAGAA GGCACAGCTT CACTTAGACC AAGTGCTCAC TAGTCCTTTC 
AACACGACAT TTCCACTAGA TTACATGAAA GATGAGTTCC TCCCAGCCCT GTCTTTGCAG 
AGTGGCGCTT TACATGGCAG TTCTGAGAGC CTGAAGCCCG AAGGGGTGGC AGATTCTCCG 
TGTGGCAGCC TGATGGAAGG GACCCCTCAG ATTAGTTCAG AGGAAAGCTA TAAACATGAG 
GGCCTAGCCG AGACCCCCGA GACAAGCCCA GAGAGCCTTT CCTTCTCGCC AAAGAAAAGT 
GAGGAGCAGA TTGGGGAAAC ACATGAAACT ACCAAGTTAG AAACAGCACC AGAAAGTCAT 
TCCGAAAAAG GACATCCCTC AACCAAAGAT GTTGCTGATG GTTCTGAAGG GCAAGGTGCT 
GCAGTCACCG AGGCAGTAGA GTCCTCTGCT GAGCATCAGC TGACAGAGGC CACCCCAGAC 
CCTTCCAAAG ACATATGCCC CCCACAGGAA GGTGACAGCC TTGGTGGTCA AAGTGTATCA 
TTAGCAAAAG AAACATCCGA AGGACTGACT GAGGAAGTGA CCTGTGACGA AGGTCCACGT 
GCACACATTA GTCCATCCCA CGAAACACAA ACTGATACCG AAGCTCAGGA ATCCACAGCC 
ACCAAGCCCT CAGGGGAGGC AGAGGCCTCA CCGTTGTCCG AGGCTGCTGT AAAGGCAGGT 
TCAGGGACTG AATCAAAGCC CCAGGCAGCC ATTAGAAGTC CGCAAGGGTT AGAACTCGCA 
CTCCCTAGCC GCGAGAGTGA GGTTTTCAGC CCTGGGGCTG ATGAATCCTT CGCTGTGAGC 
CACAAAGACT CCCTGGAGGC CAGCCCTGTG CTGGAAGATA ACTCTTCGCA CAAAACTCCC 
GATTCTCTGG AACCAAGTCC TCTGAAGGAA TCCCCTTGCC GTGACTCTCT TGAAAGCAGC 
CCCGTTGAAC CAAAGATGAA GGCTGGAATT CTCCCAAGTC ACTTTCCTCT TCCCTCAGCT 
GTTGCCAAGA CCGAGCTCTT CACGGAAGTG GCCTCTGTGC GGTCCCGGCT GCTCCGAGAC 
CCCGACGGCA GTGCCGAGGA TGACAGCCTC GAGCAGACGT CACTCATGGA GAGCTCGGGG 
AAGAGTCCCC TTTCCCCAGA CACCCCCAGC TCCGAAGAAG TTAGCTACGA GGTCACGCCC 
AAAACCACAG ACACACCAAA ACCAGCTGTG ATTCCTGAAT GTGCGGAGGA GGACGACTCA 
GAAAATGGGG AGAAAAAGAG GTTCACACCG GAAGAGGAAA TGTTTAAAAT GGTAACCAAA 
GTCAAAACGT TTGATGAACT TGAACAAGAA GCAAAGCAGA AAAGGGACTA CAAAAGAGAA 
CCCAAGCAGG AAGAATCTTC TTCATCCTCT GATGCAGATG TCGACTGTTC AGCAGACACG 
GATGAACCAA AACACACAGA GAGTGTCGAG GATGAAAGTG ACGCTCCTGT GGTAGTAACA 
ACAGAGGGCA GGGAGGCTTC TTCCTCCTCA GAAAGTGAAC CTGAGTTGAC ACAGCTGAAG 
AAAGGTGCTG ACTCAGGTCT CTTGGCAGAG CCAGTTATTC GAGTGCAGCC TCCTTCCCCA 
CTTCCATCCA GCATAGACTC AAATTCCAGT CCTGAAGAAA TACAATTCCA GCCTATCGTT 
TCCAAACAGT ACACTTTTAA GATGAATGAA GATGCTCAGG AAGATTCAGA TAAATCAGAA 
GAAGAAAAAG ATTATAAATC TCGCTTACCC GAAGACAGTC ATGCTCTTCC CACTGATGGA 
CCAGATATGT CTTATGATGA TCTGAATGGA GATACTTATC AATCAAAACT TTGTGATGGC 
CTTGAGTATG AGATTGGGAG TCCTAGCGAC TCAGCTGCTC CTGTCTCTTC AGGTCTCCGC 
AGTTCCCCTG GTGACGATGT CAGTGAGCAG CTAGTTATCC ATAAGGAATC ACTAGCCCTC 
CAAGACACTG GTGAGAACGA CCTAGAAGAG CTTGATGTTC CTGAAGTCGA ATCTTCACAA 
GCAGATCCCC CCAGTGAAGG CTCTCCATCT TTCTCCTCTT TACCTCTTTG TCCAGCATCT 
GAAGCAAAAG AATTAGATGA AGACATGGTC TCTACAGCTT CTGCTACAGA AATGGAGGTC 
ACAAAACCTG ACCAAGCTTT TGAGAGCTTA CCAGAGGACT GTTCCATTCA AGATTCACTC 
ATTACTACTC AAACTGATAG ATCATCCTTG GATGTTCCAG TGTCAGACCC AACAGAGACT 
GAGGAAATCT ATGATCCTCA AATCATAAGC CCTTATGAAA ATGTTCCTTC TCAATCTTTT 
TTCTCTGGTG AAGAAAGCAA AACCCAAACG GATACAAGGC ACACAAGTTT TCATTCTTCT 
GAAGAGTATT CTGTTACCAT CACATCCTCT GTTGAAGATG TAGTAGAGAC AAGCTCCTCT 
GATAAAACTG TTTCAAGTAA AGAAGCTAAT TTTGAGGAGC AGGTCTTGGA AGTAGAATCC 
AAACAAGAGA GTACCTTGCA GGAAATACAG TCACATGGAG CCTCCTCATC TTTGGAGCCA 
CCTGTACCAA AAACATCAAC AGTTGTCAGT GAACAAATAA GCAAAGTCAT CATCACAAAA 
ACTGATGTGG ATTCTGATTC CTGGAGTGAA ATCCGTGAGG ATGACGAAGC CTTTGAGGCT 
CGAGTGAAAG AGGAAGAACA GAAGATATTT GGTTTGATGG TAGACAGACA GTCACAGGGT 
ACCACCCCTG ACACCACTCC TGCTAGGACC CCAACTGAAG AGGGGACCCC AACCAGTGAG 
CAAAATCCAT TTCTCTTTCA GGAAGGAAAA TTGTTTGAGA TGACCCGAAG TGGTGCCATT 
GATATGACCA AAAGGTCCTA TGCCGATGAA AGCTTTCACT TTTTCCAAAT TGGGCAAGAA 
TCCAGGGAAG AGGCTGTCTC ACAAGACATG AAAGAAGGAG GTACAGGTGC TGAGGCCCCA 
CAGGTGGAGA CATCAGCTGA GTCATTAACA CTCTCAGAAT CAAAAGAAAC AGTGGATGAT 
GAAGCAGAGT TACTCCCAGA TGACCTGAGT GAGGAGGTAG AAGAAATACA CACTTCAGAT 
ACCCCACTTA GCTCCCAAAT AGACATTTCA GCCTCCACAG AAACATCCAT GAAAGAGGCT 
ACTTCTACAG GGCTTGAGGA CCTACCCACC AGGCAAATGG GTGATTCTCT TCCTCCATCC 
AGCGAGAAGC AGACCACTTG CCCTGACTTC CCTGAACAAG TTGTACAAGT TCAGTTAGAT 
TTTTCCACAG TCACTAGGTC TGTATATTCA GATCGGGGTG ATGATTCTCC CGATTCCTCC 
CCAGAGGAAC AGAAATCAGT CATTGAAATC CCTACTGCAA CCATGGAGAA TGTGCCTTCT 
ACTGAGAGCA AATCCAAAAT CCCTGTAAGG ACTATCCCCA CTTCAACCTC AATACCTCCA 
TCGGTGGAAT ATGAGAGTTC CCTTTCTGAA GATTTTCTGC CCAGCCTGGA TGAGGAAAGT 
AAAGAAGATG AAGCAAAACC AAAGTCTAAA ATCCCCGTGA AAGCACCTGC CCAAAGAGTG 
GAGCAGCAGC CTTCACATCT AGACTCACCT CTAAAGAAGA CAGGTCGTCC CCAGGGACAG 
GACTTGACAA GCAGTGCACC AGATGATAGA AGCAAATCTG AATCTGATGC CAGTTTGGAC 
GTGAAGACCA AATGTCCAGT AGAAACTAGA AGTTACACTG AGGCAGAAAT AGAAAGCACA 
GGTGAGGAAG AGGAGCTTGA GTTAGAATCA GAGGAAGGGG CCACAAGACC AAAGATATTT 
GCATCCCGAT TGCCAGTAAA GAGCAAAAGC ACCGCATCTT CCTGCAGGGG GCCCACAAGC 
CCCACAAAAG ACAATAAGGA GCATTTCTTT GATCTTTACA GGAACTCCAT AGAATTCTTT 
GAGGAGATTA GTGATGAAGC TTCCAAGTTA GTGGATAGAC TTACACAGTC AGAAAGGGAG 
CAAGATTTAG TTTCAGATGA TGAAAGTAGT AGTGCCCTGG AAGTTTCAGT AATTGAAAAT 
CTGCCACCTG TTGAGACCGA GAACTCAATT CCTGAGGACC TCTTTGACAC AAGGCCCATT 
TGGGATGAGT CCATTGAGAC TCTGATCGAA CGCATCCCTG ATGAAAATGG CCATGACCAT 
GCTGAAGATC AACAGGATGA GCAGGAACGG ATTGAGGAAA GGCTGGCATA TATTGCTGAT 
CACCTTGGCT TCAGCTGGAC AGAGTTGGCA AGAGAACTGG ACTTCACTGA AGAGCAAATT 
CATCAAATTC GAATTGAGAA CCCTAACTCC CTGCAGGATC AGAGTCATGC GCTGTTGAAG 
TACTGGCTAG AAAGGGACGG GAGACATGCT ACAGATACCA GTCTCGTTGA ATGTCTCACC 
AAGATCAACC GAATGGATAT CGTGCATCTC ATGGAGACCA GCACAGAAGC TCTCCAGGAG 
CGCATCGGTC ATAGCTATGC AGAGATTGAG CAGACCATTA CACTGGATCA TAGTGAAGGG 
TTCTCAGTAC TCCAAGAGGA GCTTTGCACT GCCCAGCACA AGCAGAAAGA GGAGCATGCT 
GCTTCTAAAG AGGACGAGGC CTCTGACCAC CCTCCCATCG TCTCAGAGGA AGACATTTCT 
GTTGGTTACT CCACTCTTCA GGATGGCATC CCCAAAACTG AGGGTGATGG CTCTGCAACA 
GAGCTCCTTC CCCAGACTCA AAAGGAGCAA GTTCAACAGG ATTTCTCAGG GAAAATGCAA 
GACCTGCCTG AGGAGTCGGC TCTTGAACAG CAAGAATACT TCGTGACAAC CCCAGGGACA 
GAAATGTCAG AGACTCAGAA GGCCACGGGA ACATCGGGCT CCCCCACCAA GACACCTGAG 
GAAATTGTCA CCCCTTCCGA GGAGGAGAGG CTGTATCTCC AAACCCCAAC ATCGAGTGAG 
CGGGGAGGCT CTCCAGTTGT GCAGGAACCT GAGGAGCCCC CCGAACGCAG GGAGGAGAGT 
TCACCTCGGA AAACCAGCCT CGTGATCGTG GAATCGGCTG AGGACCAGCC TCAGGCCTTC 
GAGAGGCTTG ATGAAGAGGC TGCTTTTCAA AAGGAGCTAA CAGAGGAATT AGGTGAGCTG 
GAGGCCAGCT CAGATGAGGA GGCGATGGTA ACAACCAGGG TTGTCCGCCG ACGATTGATT 
ATTCAGGGAG ATGATATGCC TGACATACCC CCAGAAACAG TCACAGAAGA AGAATACATC 
GATGAGCATG GACACACCGT GGTAAAGAAG GTTACTAGGA AAATCATTAG GCGGTTCATC 
TCCTCTGACG GCACAGAGAA AGAGGAGATT ACGATGCAGG GAGCACCACA GGATTCTATC 
AGCGTTGATG AAGGGGATGG TTATTCCAAA GTCATAAAGC GCATTGTATT GAAGAGTGAC 
ACCGAGCAGT CAGAGGTAAC TTTGTCTGAG CCCAGCATTC TGTCCAGTAC CTCACAATTT 
CAGGCTGAAC CAGTGGAAGG CCGTAGAGTC AGCAAAGTTG TTAAGACAAC TGTGGTCCAT 
GGTGAGAGGA TGGAGAAACA TCTGGGGGAT TCTAGTTTAG CCACTGATCT TCCTTCAGCC 
AAAGATGACT TTGAAGAGGC TTTGAGTTAC ACAGGTAGCC ACATGAAAGT CTACTCCCCC 
AGTTTAGTAG AGAATGAAGT CCTGAAGGAG GATGGATCAA TAATTAGAAG GACGACAATG 
AGTAAAGCTA GAACCCAGAA GAGGGCTGTG GTGAAGGACC AGCAGGGAAA ACGCATTTAC 
TTGGAGCACC TGGAGGATGT CCCAGAAGCG CTAGACCAGG ACGACCTCCA GCACGACCTC 
CAGCAACTCC TTCGGCATTT CTGCAAGGAG GACTCGAAGC AAGAGGCCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK2 ( XM_010805941.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804243.1 Bos taurus ankyrin-2 (LOC539238), transcript variant X1, mRNA.

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CloneID	OBa54238
Clone ID Related Accession (Same CDS sequence)	XM_010805941.2
Accession Version	XM_010805941.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103961.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805941.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGACTGAGG TTCTTGATGT TTCTGATGAA GAGGCTTTTA AACAGTTTGG TGACCACTTT 
ATTGATGGGG AAGTTCTTAG TGATTCAGGT GATGACACAA TGACGGGTGA TGGGGGAGAG 
TACCTTAGAC CTGAGGACCT AAAGGAGCTG GGTGATGACT CCCTCCCCAG CAGTCAGTTC 
CTGGACGGGA TGAATTACCT GCGCTACAGC TTGGAGGGGG GCCGCTCCGA CAGCCTTCGA 
TCCTTCAGTT CCGACAGGTC TCACACCCTG AGCCATGCCT CGTACCTGAG GGACAGTGCC 
ATGATCGACG ACGCAGTTGT GATCCCCAGT CACCAGGTGA CAACTCTAGC CAAGGAGGCA 
GAAAGGAATT CTTATCGTCT AAGCTGGGGC ACTGAGAACT TGGACAACGT GGCTCTTTCT 
TCCAGCCCTA TTCATTCAGG CCGCACCTCT CCATGTCTTG ATCGTGACAA CAGCAGTTTC 
CTGGTTAGTT TTATGGTGGA TGCCCGAGGT GGTGCTATGC GAGGATGCAG ACACAACGGG 
CTCCGCATCA TTATTCCGCC CCGGAAATGT ACCGCCCCAA CACGAGTCAC CTGCCGACTG 
GTCAAACGCC ATAGACTGGC GACAATGCCT CCAATGGTGG AAGGAGAGGG CCTGGCCAGT 
CGCCTGATTG AAGTTGGACC TTCTGGTGCT CAGTTCCTTG GTAAACTTCA CCTGCCAACG 
GCTCCTCCCC CACTTAATGA GGGAGAAAGT TTGGTCAGCC GCATCCTTCA GCTGGGGCCT 
CCTGGAACCA AATTCCTTGG GCCTGTGATC GTGGAGATCC CTCATTTCGC TGCCCTGCGA 
GGAAAAGAGA GGGAACTGGT GGTCCTGCGC AGTGAGAACG GGGACAGCTG GAAAGAGCAT 
TACTGTGAAT ACACGGAGGA GGAGCTGAAT GAAATCCTGA ACGGCATGGA TGAAGTGCTG 
GATAGCCCGG AAGACCTCGA AAAGAAGCGG ATCTGCCGCA TCATCACCCG TGACTTCCCA 
CAGTACTTTG CGGTGGTGTC GCGCATCAAG CAGGACAGCA ACCTGATTGG CCCGGAGGGA 
GGCGTGCTGA GCAGCACCGT GGTGCCGCAG GTGCAGGCCG TGTTCCCCGA GGGGGCGCTA 
ACCAAGCGGA TCCGTGTTGG CCTACAGGCT CAACCTATGC ACAGTGAGTT GGTTAAGAAG 
ATTCTGGGCA ACAAAGCTAC CTTCAGCCCT ATAGTTACTT TGGAACCTAG AAGAAGAAAA 
TTTCACAAGC CAATTACCAT GACCATTCCT GTCCCCAAAG CTTCAAGTGA TGTCATGTTG 
AATGGTTTTG GAGGAGATGC ACCAACCTTA AGATTACTAT GTAGCATCAC AGGTGGAACC 
ACCCCTGCTC AGTGGGAAGA TATTACAGGA ACTACGCCAT TAACATTTGT CAATGAATGT 
GTTTCCTTTA CAACCAATGT GTCTGCCAGG TTCTGGCTGA TCGATTGTCG ACAAATTCAG 
GAATCTGTTA CCTTTGCATC ACAAGTGTAT AGAGAAATTA TCTGTGTACC ATATATGGCC 
AAATTTGTAG TGTTTGCCAA ATCACATGAC CCCATTGAAG CCAGGTTGAG GTGTTTCTGC 
ATGACTGATG ATAAAGTGGA TAAAACCCTG GAACAACAAG AAAACTTTGC TGAGGTGGCC 
AGAAGCAGGG ATGTGGAGGT CTTAGAAGGA AAACCCATCT ATGTTGATTG TTTTGGCAAC 
CTGGTGCCAT TAACCAAGAG TGGCCAACAT CATATATTCA GTTTTTTTGC CTTCAAAGAA 
AACAGACTTC CTCTCTTTGT CAAGGTACGT GATACGACTC AGGAACCTTG CGGACGACTA 
TCATTTATGA AAGAGCCAAA ATCTACAAGA GGCCTGGTGC ACCAAGCTAT TTGCAACTTA 
AACATCACCC TGCCAATTTA TACGAAGGAA TCAGAGTCAG ATCAAGAACA GGAGGAAGAG 
ATCGATATGA CATCAGAAAA AAATGATGAG ACAGAATCTA CAGAAACATC TGTCCTGAAA 
AGTCACCTGG TTAATGAAGT TCCTGTCCTG GCAAGTCCGG ACTTGCTCTC TGAAGTTTCT 
GAGATGAAAC AAGATTTGAT CAAAATGACC GCCATCTTGA CCACAGATGG ATCTGATAGG 
GCAGGTTCCA TCAAAGTGAA GGAGCTGGTG AAGGCTGCAG AGGAAGAGCC AGGGGAGCCT 
TTTGAAATTG TTGAAAGAGT TAAAGAGGAC TTAGAGAAGG TGAACGAAAT CCTGAGAAGC 
GGAACCTGCA CAGGAGATGA AGGCAGTGTG CCGAGGTCTC AGCCAGAGAG AGAGTTAGTG 
GAGGAGGAGT GGGTCATTGT CAGTGAGGAG GAGATAGAGG AGGCTAAGCA AAAAGCATCT 
TTAGAGATCG CCGAATACCC ATGTGTAGAA GTTAGACTAG ATAAAGAGAC CAAAGTAGAA 
GTAGAGAAAG ACTCAACGGG GCTAGTGAAC TACCTTACTG AGGATCTGAA TTCCTATGTG 
CCTCCTCACG AAGAGCAGCC CCAGAAGGAG CAAGGTATGG TAGGGGAGAA GCCTGAGGCT 
CAGGGTCCCA GCAGGCGCTC AGAAAGCGAA GGCAAAGATG CACCCTCAGA GGAGACACAG 
AGTACACAGA AACAGCCCAA ACCAAGCCTG GGGATCAAGA AGCCGGTGAG AAGGAAATTA 
AAGGAGAAAC AAAAGCAGAA GGAGGACAGT CCACAAGCTA GTGCAGAAAA AACAGAACTT 
AAAAAAGGGA GTTCAGAAGA GTCATTAGAT GAAGACACAA GTTCAGCCCC CGAGCCTCTT 
CCTGCTGTAA AAGCTACGTC ACCTTTGATA GAGGAAACGC CCATTGGTTC CATAAAGGAC 
AAGGTGAAGG CCCTTCAGAA ACGAGTGGAA GATGAACAGA AGGGTCGAAG CAAGTTGCCT 
GTCAGAGTCA AAGGCAAAGA GGATGTGCCA AAAAAGGTAA CACAAAGGAC ACATTTGACT 
GCATCGCCCT CTCTGAAGTC AGAGAGGCAT GCGCCAGCGT CCGCCTCCTC TAGAACAGAA 
AGACACACTT CTCTTTCCTC CTCTGCCAAA ACAGAAAGGC ACCCTCCGGT ATCACCATCG 
AGTAAGAACG AGAAACACTC GCCTGTGTCA CCCTCCGCGA AAGCTGAAAA GCAGTCCCCT 
GTGTCATCCT CAAGCAAAAC TGAGAAACAC TCACCCGTTT CACCCTCGAC CAAAACGGAA 
AGACACTCCC CTATGTCCTC TTCTACAAAA ACAGAAAGAC ACCCTCCTGT TTCGCCTTCA 
GGTAAGACAG ATAAACGTCC CCCTGGATCT CCCTCTGGGA GATCCGAGAA ACATCCGCCG 
GTATCTCCTG GCAGAACAGA GAAACGCTTG CCGGTGTCAC CCGCTGGAAG GATGGAAAAG 
CATTCTCCTG TCTCAAGCTC CGGGAAAACT GAGAAGCACC TGCCCGTGTC ACCTTCTGGG 
AAAACAGACA AACAACCACC TGTCTCCCCC ACCTCAAAAA CAGAAAGAAT CGAGGAGACC 
ATGTCTGTTC GGGAGCTGAT GAAAGCTTTC CAGTCAGGTC AGGACCCATC GAAACACAAG 
ACTGGACTTT TTGAGCACAA ATCTGCAAAA CCAAAACAGC CACAAGAAAA GGGTAGAGGT 
CGGCTAGAAA AAGAAAAGGG GCCGATAGTA ACCCCAAGAG AAGCGCAGAA AACGGAGAGT 
CAGACAATCA AACGAGGCCA GAGGTTCCTG GTCACGGGAT TCTCAGAACC CAGAAGAGCA 
GTCCGTGCTT CCTCCACAGG GTTTAAGAGA GAAGATGGGG CTGGAGAAAA GGAGAAATCT 
CTCAACCACA AAACACCCGA ACCTGTTCAG CCAGTGCCTG AAGAAGAAAG CCATAAAGAT 
AGCGAAGTCC CCAAAGAAAA GCTGGCTGAC GACCAGGGAG ACATGGATCT CCAGATCAGC 
CCAGACCGGA AAACCTCCAC TGACTTTTCT GACGTCATTA AGCAAGAACT GGAAGACAAT 
GACAAATACC AGCAGTTCCG TATGACTGAG GAGCCTGAGA AGGCACAGCT TCACTTAGAC 
CAAGTGCTCA CTAGTCCTTT CAACACGACA TTTCCACTAG ATTACATGAA AGATGAGTTC 
CTCCCAGCCC TGTCTTTGCA GAGTGGCGCT TTACATGGCA GTTCTGAGAG CCTGAAGCCC 
GAAGGGGTGG CAGATTCTCC GTGTGGCAGC CTGATGGAAG GGACCCCTCA GATTAGTTCA 
GAGGAAAGCT ATAAACATGA GGGCCTAGCC GAGACCCCCG AGACAAGCCC AGAGAGCCTT 
TCCTTCTCGC CAAAGAAAAG TGAGGAGCAG ATTGGGGAAA CACATGAAAC TACCAAGTTA 
GAAACAGCAC CAGAAAGTCA TTCCGAAAAA GGACATCCCT CAACCAAAGA TGTTGCTGAT 
GGTTCTGAAG GGCAAGGTGC TGCAGTCACC GAGGCAGTAG AGTCCTCTGC TGAGCATCAG 
CTGACAGAGG CCACCCCAGA CCCTTCCAAA GACATATGCC CCCCACAGGA AGGTGACAGC 
CTTGGTGGTC AAAGTGTATC ATTAGCAAAA GAAACATCCG AAGGACTGAC TGAGGAAGTG 
ACCTGTGACG AAGGTCCACG TGCACACATT AGTCCATCCC ACGAAACACA AACTGATACC 
GAAGCTCAGG AATCCACAGC CACCAAGCCC TCAGGGGAGG CAGAGGCCTC ACCGTTGTCC 
GAGGCTGCTG TAAAGGCAGG TTCAGGGACT GAATCAAAGC CCCAGGCAGC CATTAGAAGT 
CCGCAAGGGT TAGAACTCGC ACTCCCTAGC CGCGAGAGTG AGGTTTTCAG CCCTGGGGCT 
GATGAATCCT TCGCTGTGAG CCACAAAGAC TCCCTGGAGG CCAGCCCTGT GCTGGAAGAT 
AACTCTTCGC ACAAAACTCC CGATTCTCTG GAACCAAGTC CTCTGAAGGA ATCCCCTTGC 
CGTGACTCTC TTGAAAGCAG CCCCGTTGAA CCAAAGATGA AGGCTGGAAT TCTCCCAAGT 
CACTTTCCTC TTCCCTCAGC TGTTGCCAAG ACCGAGCTCT TCACGGAAGT GGCCTCTGTG 
CGGTCCCGGC TGCTCCGAGA CCCCGACGGC AGTGCCGAGG ATGACAGCCT CGAGCAGACG 
TCACTCATGG AGAGCTCGGG GAAGAGTCCC CTTTCCCCAG ACACCCCCAG CTCCGAAGAA 
GTTAGCTACG AGGTCACGCC CAAAACCACA GACACACCAA AACCAGCTGT GATTCCTGAA 
TGTGCGGAGG AGGACGACTC AGAAAATGGG GAGAAAAAGA GGTTCACACC GGAAGAGGAA 
ATGTTTAAAA TGGTAACCAA AGTCAAAACG TTTGATGAAC TTGAACAAGA AGCAAAGCAG 
AAAAGGGACT ACAAAAGAGA ACCCAAGCAG GAAGAATCTT CTTCATCCTC TGATGCAGAT 
GTCGACTGTT CAGCAGACAC GGATGAACCA AAACACACAG AGAGTGTCGA GGATGAAAGT 
GACGCTCCTG TGGTAGTAAC AACAGAGGGC AGGGAGGCTT CTTCCTCCTC AGAAAGTGAA 
CCTGAGTTGA CACAGCTGAA GAAAGGTGCT GACTCAGGTC TCTTGGCAGA GCCAGTTATT 
CGAGTGCAGC CTCCTTCCCC ACTTCCATCC AGCATAGACT CAAATTCCAG TCCTGAAGAA 
ATACAATTCC AGCCTATCGT TTCCAAACAG TACACTTTTA AGATGAATGA AGATGCTCAG 
GAAGATTCAG ATAAATCAGA AGAAGAAAAA GATTATAAAT CTCGCTTACC CGAAGACAGT 
CATGCTCTTC CCACTGATGG ACCAGATATG TCTTATGATG ATCTGAATGG AGATACTTAT 
CAATCAAAAC TTTGTGATGG CCTTGAGTAT GAGATTGGGA GTCCTAGCGA CTCAGCTGCT 
CCTGTCTCTT CAGGTCTCCG CAGTTCCCCT GGTGACGATG TCAGTGAGCA GCTAGTTATC 
CATAAGGAAT CACTAGCCCT CCAAGACACT GGTGAGAACG ACCTAGAAGA GCTTGATGTT 
CCTGAAGTCG AATCTTCACA AGCAGATCCC CCCAGTGAAG GCTCTCCATC TTTCTCCTCT 
TTACCTCTTT GTCCAGCATC TGAAGCAAAA GAATTAGATG AAGACATGGT CTCTACAGCT 
TCTGCTACAG AAATGGAGGT CACAAAACCT GACCAAGCTT TTGAGAGCTT ACCAGAGGAC 
TGTTCCATTC AAGATTCACT CATTACTACT CAAACTGATA GATCATCCTT GGATGTTCCA 
GTGTCAGACC CAACAGAGAC TGAGGAAATC TATGATCCTC AAATCATAAG CCCTTATGAA 
AATGTTCCTT CTCAATCTTT TTTCTCTGGT GAAGAAAGCA AAACCCAAAC GGATACAAGG 
CACACAAGTT TTCATTCTTC TGAAGAGTAT TCTGTTACCA TCACATCCTC TGTTGAAGAT 
GTAGTAGAGA CAAGCTCCTC TGATAAAACT GTTTCAAGTA AAGAAGCTAA TTTTGAGGAG 
CAGGTCTTGG AAGTAGAATC CAAACAAGAG AGTACCTTGC AGGAAATACA GTCACATGGA 
GCCTCCTCAT CTTTGGAGCC ACCTGTACCA AAAACATCAA CAGTTGTCAG TGAACAAATA 
AGCAAAGTCA TCATCACAAA AACTGATGTG GATTCTGATT CCTGGAGTGA AATCCGTGAG 
GATGACGAAG CCTTTGAGGC TCGAGTGAAA GAGGAAGAAC AGAAGATATT TGGTTTGATG 
GTAGACAGAC AGTCACAGGG TACCACCCCT GACACCACTC CTGCTAGGAC CCCAACTGAA 
GAGGGGACCC CAACCAGTGA GCAAAATCCA TTTCTCTTTC AGGAAGGAAA ATTGTTTGAG 
ATGACCCGAA GTGGTGCCAT TGATATGACC AAAAGGTCCT ATGCCGATGA AAGCTTTCAC 
TTTTTCCAAA TTGGGCAAGA ATCCAGGGAA GAGGCTGTCT CACAAGACAT GAAAGAAGGA 
GGTACAGGTG CTGAGGCCCC ACAGGTGGAG ACATCAGCTG AGTCATTAAC ACTCTCAGAA 
TCAAAAGAAA CAGTGGATGA TGAAGCAGAG TTACTCCCAG ATGACCTGAG TGAGGAGGTA 
GAAGAAATAC ACACTTCAGA TACCCCACTT AGCTCCCAAA TAGACATTTC AGCCTCCACA 
GAAACATCCA TGAAAGAGGC TACTTCTACA GGGCTTGAGG ACCTACCCAC CAGGCAAATG 
GGTGATTCTC TTCCTCCATC CAGCGAGAAG CAGACCACTT GCCCTGACTT CCCTGAACAA 
GTTGTACAAG TTCAGTTAGA TTTTTCCACA GTCACTAGGT CTGTATATTC AGATCGGGGT 
GATGATTCTC CCGATTCCTC CCCAGAGGAA CAGAAATCAG TCATTGAAAT CCCTACTGCA 
ACCATGGAGA ATGTGCCTTC TACTGAGAGC AAATCCAAAA TCCCTGTAAG GACTATCCCC 
ACTTCAACCT CAATACCTCC ATCGGTGGAA TATGAGAGTT CCCTTTCTGA AGATTTTCTG 
CCCAGCCTGG ATGAGGAAAG TAAAGAAGAT GAAGCAAAAC CAAAGTCTAA AATCCCCGTG 
AAAGCACCTG CCCAAAGAGT GGAGCAGCAG CCTTCACATC TAGACTCACC TCTAAAGAAG 
ACAGGTCGTC CCCAGGGACA GGACTTGACA AGCAGTGCAC CAGATGATAG AAGCAAATCT 
GAATCTGATG CCAGTTTGGA CGTGAAGACC AAATGTCCAG TAGAAACTAG AAGTTACACT 
GAGGCAGAAA TAGAAAGCAC AGGTGAGGAA GAGGAGCTTG AGTTAGAATC AGAGGAAGGG 
GCCACAAGAC CAAAGATATT TGCATCCCGA TTGCCAGTAA AGAGCAAAAG CACCGCATCT 
TCCTGCAGGG GGCCCACAAG CCCCACAAAA GACAATAAGG AGCATTTCTT TGATCTTTAC 
AGGAACTCCA TAGAATTCTT TGAGGAGATT AGTGATGAAG CTTCCAAGTT AGTGGATAGA 
CTTACACAGT CAGAAAGGGA GCAAGATTTA GTTTCAGATG ATGAAAGTAG TAGTGCCCTG 
GAAGTTTCAG TAATTGAAAA TCTGCCACCT GTTGAGACCG AGAACTCAAT TCCTGAGGAC 
CTCTTTGACA CAAGGCCCAT TTGGGATGAG TCCATTGAGA CTCTGATCGA ACGCATCCCT 
GATGAAAATG GCCATGACCA TGCTGAAGGT ATTTGCCAGC CACTGGGATT CCCTGTGCTA 
CGCATGTCAG TCACCAGGAC CAAGACAAAC GTTGTTTCTG GCCATACGGA AAAAACCGGG 
ATGGTCCGAT CAACCGAGTT GGCAAGAGAA CTGGACTTCA CTGAAGAGCA AATTCATCAA 
ATTCGAATTG AGAACCCTAA CTCCCTGCAG GATCAGAGTC ATGCGCTGTT GAAGTACTGG 
CTAGAAAGGG ACGGGAGACA TGCTACAGAT ACCAGTCTCG TTGAATGTCT CACCAAGATC 
AACCGAATGG ATATCGTGCA TCTCATGGAG ACCAGCACAG AAGCTCTCCA GGAGCGCATC 
GGTCATAGCT ATGCAGAGAT TGAGCAGACC ATTACACTGG ATCATAGTGA AGGGTTCTCA 
GTACTCCAAG AGGAGCTTTG CACTGCCCAG CACAAGCAGA AAGAGGAGCA TGCTGCTTCT 
AAAGAGGACG AGGCCTCTGA CCACCCTCCC ATCGTCTCAG AGGAAGACAT TTCTGTTGGT 
TACTCCACTC TTCAGGATGG CATCCCCAAA ACTGAGGGTG ATGGCTCTGC AACAGAGCTC 
CTTCCCCAGA CTCAAAAGGA GCAAGTTCAA CAGGATTTCT CAGGGAAAAT GCAAGACCTG 
CCTGAGGAGT CGGCTCTTGA ACAGCAAGAA TACTTCGTGA CAACCCCAGG GACAGAAATG 
TCAGAGACTC AGAAGGCCAC GGGAACATCG GGCTCCCCCA CCAAGACACC TGAGGAAATT 
GTCACCCCTT CCGAGGAGGA GAGGCTGTAT CTCCAAACCC CAACATCGAG TGAGCGGGGA 
GGCTCTCCAG TTGTGCAGGA ACCTGAGGAG CCCCCCGAAC GCAGGGAGGA GAGTTCACCT 
CGGAAAACCA GCCTCGTGAT CGTGGAATCG GCTGAGGACC AGCCTCAGGC CTTCGAGAGG 
CTTGATGAAG AGGCTGCTTT TCAAAAGGGA GATGATATGC CTGACATACC CCCAGAAACA 
GTCACAGAAG AAGAATACAT CGATGAGCAT GGACACACCG TGGTAAAGAA GGTTACTAGG 
AAAATCATTA GGCGGTTCAT CTCCTCTGAC GGCACAGAGA AAGAGGAGAT TACGATGCAG 
GGAGCACCAC AGGATTCTAT CAGCGTTGAT GAAGGGGATG GTTATTCCAA AGTCATAAAG 
CGCATTGTAT TGAAGAGTGA CACCGAGCAG TCAGAGGTAA CTTTGTCTGA GCCCAGCATT 
CTGTCCAGTA CCTCACAATT TCAGGCTGAA CCAGTGGAAG GCCGTAGAGT CAGCAAAGTT 
GTTAAGACAA CTGTGGTCCA TGGTGAGAGG ATGGAGAAAC ATCTGGGGGA TTCTAGTTTA 
GCCACTGATC TTCCTTCAGC CAAAGATGAC TTTGAAGAGG CTTTGAGTTA CACAGGTAGC 
CACATGAAAG TCTACTCCCC CAGTTTAGTA GAGAATGAAG TCCTGAAGGA GGATGGATCA 
ATAATTAGAA GGACGACAAT GAGTAAAGCT AGAACCCAGA AGAGGGCTGT GGTGAAGGAC 
CAGCAGGGAA AACGCATTTA CTTGGAGCAC CTGGAGGATG TCCCAGAAGC GCTAGACCAG 
GACGACCTCC AGCACGACCT CCAGCAACTC CTTCGGCATT TCTGCAAGGA GGACTCGAAG 
CAAGAGGCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804243.1
CDS	377..10291
Translation	MTEVLDVSDEEAFKQFGDHFIDGEVLSDSGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSHTLSHASYLRDSAMIDDAVVIPSHQVTTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGRTSPCLDRDNSSFLVSFMVDARGGAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGKLHLPTAPPPLNEGESLVSRILQLGPPGTKFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHYCEYTEEELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEIDMTSEKNDETESTETSVLKSHLVNEVPVLASPDLLSEVSEMKQDLIKMTAILTTDGSDRAGSIKVKELVKAAEEEPGEPFEIVERVKEDLEKVNEILRSGTCTGDEGSVPRSQPERELVEEEWVIVSEEEIEEAKQKASLEIAEYPCVEVRLDKETKVEVEKDSTGLVNYLTEDLNSYVPPHEEQPQKEQGMVGEKPEAQGPSRRSESEGKDAPSEETQSTQKQPKPSLGIKKPVRRKLKEKQKQKEDSPQASAEKTELKKGSSEESLDEDTSSAPEPLPAVKATSPLIEETPIGSIKDKVKALQKRVEDEQKGRSKLPVRVKGKEDVPKKVTQRTHLTASPSLKSERHAPASASSRTERHTSLSSSAKTERHPPVSPSSKNEKHSPVSPSAKAEKQSPVSSSSKTEKHSPVSPSTKTERHSPMSSSTKTERHPPVSPSGKTDKRPPGSPSGRSEKHPPVSPGRTEKRLPVSPAGRMEKHSPVSSSGKTEKHLPVSPSGKTDKQPPVSPTSKTERIEETMSVRELMKAFQSGQDPSKHKTGLFEHKSAKPKQPQEKGRGRLEKEKGPIVTPREAQKTESQTIKRGQRFLVTGFSEPRRAVRASSTGFKREDGAGEKEKSLNHKTPEPVQPVPEEESHKDSEVPKEKLADDQGDMDLQISPDRKTSTDFSDVIKQELEDNDKYQQFRMTEEPEKAQLHLDQVLTSPFNTTFPLDYMKDEFLPALSLQSGALHGSSESLKPEGVADSPCGSLMEGTPQISSEESYKHEGLAETPETSPESLSFSPKKSEEQIGETHETTKLETAPESHSEKGHPSTKDVADGSEGQGAAVTEAVESSAEHQLTEATPDPSKDICPPQEGDSLGGQSVSLAKETSEGLTEEVTCDEGPRAHISPSHETQTDTEAQESTATKPSGEAEASPLSEAAVKAGSGTESKPQAAIRSPQGLELALPSRESEVFSPGADESFAVSHKDSLEASPVLEDNSSHKTPDSLEPSPLKESPCRDSLESSPVEPKMKAGILPSHFPLPSAVAKTELFTEVASVRSRLLRDPDGSAEDDSLEQTSLMESSGKSPLSPDTPSSEEVSYEVTPKTTDTPKPAVIPECAEEDDSENGEKKRFTPEEEMFKMVTKVKTFDELEQEAKQKRDYKREPKQEESSSSSDADVDCSADTDEPKHTESVEDESDAPVVVTTEGREASSSSESEPELTQLKKGADSGLLAEPVIRVQPPSPLPSSIDSNSSPEEIQFQPIVSKQYTFKMNEDAQEDSDKSEEEKDYKSRLPEDSHALPTDGPDMSYDDLNGDTYQSKLCDGLEYEIGSPSDSAAPVSSGLRSSPGDDVSEQLVIHKESLALQDTGENDLEELDVPEVESSQADPPSEGSPSFSSLPLCPASEAKELDEDMVSTASATEMEVTKPDQAFESLPEDCSIQDSLITTQTDRSSLDVPVSDPTETEEIYDPQIISPYENVPSQSFFSGEESKTQTDTRHTSFHSSEEYSVTITSSVEDVVETSSSDKTVSSKEANFEEQVLEVESKQESTLQEIQSHGASSSLEPPVPKTSTVVSEQISKVIITKTDVDSDSWSEIREDDEAFEARVKEEEQKIFGLMVDRQSQGTTPDTTPARTPTEEGTPTSEQNPFLFQEGKLFEMTRSGAIDMTKRSYADESFHFFQIGQESREEAVSQDMKEGGTGAEAPQVETSAESLTLSESKETVDDEAELLPDDLSEEVEEIHTSDTPLSSQIDISASTETSMKEATSTGLEDLPTRQMGDSLPPSSEKQTTCPDFPEQVVQVQLDFSTVTRSVYSDRGDDSPDSSPEEQKSVIEIPTATMENVPSTESKSKIPVRTIPTSTSIPPSVEYESSLSEDFLPSLDEESKEDEAKPKSKIPVKAPAQRVEQQPSHLDSPLKKTGRPQGQDLTSSAPDDRSKSESDASLDVKTKCPVETRSYTEAEIESTGEEEELELESEEGATRPKIFASRLPVKSKSTASSCRGPTSPTKDNKEHFFDLYRNSIEFFEEISDEASKLVDRLTQSEREQDLVSDDESSSALEVSVIENLPPVETENSIPEDLFDTRPIWDESIETLIERIPDENGHDHAEGICQPLGFPVLRMSVTRTKTNVVSGHTEKTGMVRSTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGRHATDTSLVECLTKINRMDIVHLMETSTEALQERIGHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEHAASKEDEASDHPPIVSEEDISVGYSTLQDGIPKTEGDGSATELLPQTQKEQVQQDFSGKMQDLPEESALEQQEYFVTTPGTEMSETQKATGTSGSPTKTPEEIVTPSEEERLYLQTPTSSERGGSPVVQEPEEPPERREESSPRKTSLVIVESAEDQPQAFERLDEEAAFQKGDDMPDIPPETVTEEEYIDEHGHTVVKKVTRKIIRRFISSDGTEKEEITMQGAPQDSISVDEGDGYSKVIKRIVLKSDTEQSEVTLSEPSILSSTSQFQAEPVEGRRVSKVVKTTVVHGERMEKHLGDSSLATDLPSAKDDFEEALSYTGSHMKVYSPSLVENEVLKEDGSIIRRTTMSKARTQKRAVVKDQQGKRIYLEHLEDVPEALDQDDLQHDLQQLLRHFCKEDSKQEAQ

Target ORF information:

RefSeq Version	XM_010805941.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin-2 (LOC539238), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805941.2

ORF Insert Sequence:

ATGACTGAGG TTCTTGATGT TTCTGATGAA GAGGCTTTTA AACAGTTTGG TGACCACTTT 
ATTGATGGGG AAGTTCTTAG TGATTCAGGT GATGACACAA TGACGGGTGA TGGGGGAGAG 
TACCTTAGAC CTGAGGACCT AAAGGAGCTG GGTGATGACT CCCTCCCCAG CAGTCAGTTC 
CTGGACGGGA TGAATTACCT GCGCTACAGC TTGGAGGGGG GCCGCTCCGA CAGCCTTCGA 
TCCTTCAGTT CCGACAGGTC TCACACCCTG AGCCATGCCT CGTACCTGAG GGACAGTGCC 
ATGATCGACG ACGCAGTTGT GATCCCCAGT CACCAGGTGA CAACTCTAGC CAAGGAGGCA 
GAAAGGAATT CTTATCGTCT AAGCTGGGGC ACTGAGAACT TGGACAACGT GGCTCTTTCT 
TCCAGCCCTA TTCATTCAGG CCGCACCTCT CCATGTCTTG ATCGTGACAA CAGCAGTTTC 
CTGGTTAGTT TTATGGTGGA TGCCCGAGGT GGTGCTATGC GAGGATGCAG ACACAACGGG 
CTCCGCATCA TTATTCCGCC CCGGAAATGT ACCGCCCCAA CACGAGTCAC CTGCCGACTG 
GTCAAACGCC ATAGACTGGC GACAATGCCT CCAATGGTGG AAGGAGAGGG CCTGGCCAGT 
CGCCTGATTG AAGTTGGACC TTCTGGTGCT CAGTTCCTTG GTAAACTTCA CCTGCCAACG 
GCTCCTCCCC CACTTAATGA GGGAGAAAGT TTGGTCAGCC GCATCCTTCA GCTGGGGCCT 
CCTGGAACCA AATTCCTTGG GCCTGTGATC GTGGAGATCC CTCATTTCGC TGCCCTGCGA 
GGAAAAGAGA GGGAACTGGT GGTCCTGCGC AGTGAGAACG GGGACAGCTG GAAAGAGCAT 
TACTGTGAAT ACACGGAGGA GGAGCTGAAT GAAATCCTGA ACGGCATGGA TGAAGTGCTG 
GATAGCCCGG AAGACCTCGA AAAGAAGCGG ATCTGCCGCA TCATCACCCG TGACTTCCCA 
CAGTACTTTG CGGTGGTGTC GCGCATCAAG CAGGACAGCA ACCTGATTGG CCCGGAGGGA 
GGCGTGCTGA GCAGCACCGT GGTGCCGCAG GTGCAGGCCG TGTTCCCCGA GGGGGCGCTA 
ACCAAGCGGA TCCGTGTTGG CCTACAGGCT CAACCTATGC ACAGTGAGTT GGTTAAGAAG 
ATTCTGGGCA ACAAAGCTAC CTTCAGCCCT ATAGTTACTT TGGAACCTAG AAGAAGAAAA 
TTTCACAAGC CAATTACCAT GACCATTCCT GTCCCCAAAG CTTCAAGTGA TGTCATGTTG 
AATGGTTTTG GAGGAGATGC ACCAACCTTA AGATTACTAT GTAGCATCAC AGGTGGAACC 
ACCCCTGCTC AGTGGGAAGA TATTACAGGA ACTACGCCAT TAACATTTGT CAATGAATGT 
GTTTCCTTTA CAACCAATGT GTCTGCCAGG TTCTGGCTGA TCGATTGTCG ACAAATTCAG 
GAATCTGTTA CCTTTGCATC ACAAGTGTAT AGAGAAATTA TCTGTGTACC ATATATGGCC 
AAATTTGTAG TGTTTGCCAA ATCACATGAC CCCATTGAAG CCAGGTTGAG GTGTTTCTGC 
ATGACTGATG ATAAAGTGGA TAAAACCCTG GAACAACAAG AAAACTTTGC TGAGGTGGCC 
AGAAGCAGGG ATGTGGAGGT CTTAGAAGGA AAACCCATCT ATGTTGATTG TTTTGGCAAC 
CTGGTGCCAT TAACCAAGAG TGGCCAACAT CATATATTCA GTTTTTTTGC CTTCAAAGAA 
AACAGACTTC CTCTCTTTGT CAAGGTACGT GATACGACTC AGGAACCTTG CGGACGACTA 
TCATTTATGA AAGAGCCAAA ATCTACAAGA GGCCTGGTGC ACCAAGCTAT TTGCAACTTA 
AACATCACCC TGCCAATTTA TACGAAGGAA TCAGAGTCAG ATCAAGAACA GGAGGAAGAG 
ATCGATATGA CATCAGAAAA AAATGATGAG ACAGAATCTA CAGAAACATC TGTCCTGAAA 
AGTCACCTGG TTAATGAAGT TCCTGTCCTG GCAAGTCCGG ACTTGCTCTC TGAAGTTTCT 
GAGATGAAAC AAGATTTGAT CAAAATGACC GCCATCTTGA CCACAGATGG ATCTGATAGG 
GCAGGTTCCA TCAAAGTGAA GGAGCTGGTG AAGGCTGCAG AGGAAGAGCC AGGGGAGCCT 
TTTGAAATTG TTGAAAGAGT TAAAGAGGAC TTAGAGAAGG TGAACGAAAT CCTGAGAAGC 
GGAACCTGCA CAGGAGATGA AGGCAGTGTG CCGAGGTCTC AGCCAGAGAG AGAGTTAGTG 
GAGGAGGAGT GGGTCATTGT CAGTGAGGAG GAGATAGAGG AGGCTAAGCA AAAAGCATCT 
TTAGAGATCG CCGAATACCC ATGTGTAGAA GTTAGACTAG ATAAAGAGAC CAAAGTAGAA 
GTAGAGAAAG ACTCAACGGG GCTAGTGAAC TACCTTACTG AGGATCTGAA TTCCTATGTG 
CCTCCTCACG AAGAGCAGCC CCAGAAGGAG CAAGGTATGG TAGGGGAGAA GCCTGAGGCT 
CAGGGTCCCA GCAGGCGCTC AGAAAGCGAA GGCAAAGATG CACCCTCAGA GGAGACACAG 
AGTACACAGA AACAGCCCAA ACCAAGCCTG GGGATCAAGA AGCCGGTGAG AAGGAAATTA 
AAGGAGAAAC AAAAGCAGAA GGAGGACAGT CCACAAGCTA GTGCAGAAAA AACAGAACTT 
AAAAAAGGGA GTTCAGAAGA GTCATTAGAT GAAGACACAA GTTCAGCCCC CGAGCCTCTT 
CCTGCTGTAA AAGCTACGTC ACCTTTGATA GAGGAAACGC CCATTGGTTC CATAAAGGAC 
AAGGTGAAGG CCCTTCAGAA ACGAGTGGAA GATGAACAGA AGGGTCGAAG CAAGTTGCCT 
GTCAGAGTCA AAGGCAAAGA GGATGTGCCA AAAAAGGTAA CACAAAGGAC ACATTTGACT 
GCATCGCCCT CTCTGAAGTC AGAGAGGCAT GCGCCAGCGT CCGCCTCCTC TAGAACAGAA 
AGACACACTT CTCTTTCCTC CTCTGCCAAA ACAGAAAGGC ACCCTCCGGT ATCACCATCG 
AGTAAGAACG AGAAACACTC GCCTGTGTCA CCCTCCGCGA AAGCTGAAAA GCAGTCCCCT 
GTGTCATCCT CAAGCAAAAC TGAGAAACAC TCACCCGTTT CACCCTCGAC CAAAACGGAA 
AGACACTCCC CTATGTCCTC TTCTACAAAA ACAGAAAGAC ACCCTCCTGT TTCGCCTTCA 
GGTAAGACAG ATAAACGTCC CCCTGGATCT CCCTCTGGGA GATCCGAGAA ACATCCGCCG 
GTATCTCCTG GCAGAACAGA GAAACGCTTG CCGGTGTCAC CCGCTGGAAG GATGGAAAAG 
CATTCTCCTG TCTCAAGCTC CGGGAAAACT GAGAAGCACC TGCCCGTGTC ACCTTCTGGG 
AAAACAGACA AACAACCACC TGTCTCCCCC ACCTCAAAAA CAGAAAGAAT CGAGGAGACC 
ATGTCTGTTC GGGAGCTGAT GAAAGCTTTC CAGTCAGGTC AGGACCCATC GAAACACAAG 
ACTGGACTTT TTGAGCACAA ATCTGCAAAA CCAAAACAGC CACAAGAAAA GGGTAGAGGT 
CGGCTAGAAA AAGAAAAGGG GCCGATAGTA ACCCCAAGAG AAGCGCAGAA AACGGAGAGT 
CAGACAATCA AACGAGGCCA GAGGTTCCTG GTCACGGGAT TCTCAGAACC CAGAAGAGCA 
GTCCGTGCTT CCTCCACAGG GTTTAAGAGA GAAGATGGGG CTGGAGAAAA GGAGAAATCT 
CTCAACCACA AAACACCCGA ACCTGTTCAG CCAGTGCCTG AAGAAGAAAG CCATAAAGAT 
AGCGAAGTCC CCAAAGAAAA GCTGGCTGAC GACCAGGGAG ACATGGATCT CCAGATCAGC 
CCAGACCGGA AAACCTCCAC TGACTTTTCT GACGTCATTA AGCAAGAACT GGAAGACAAT 
GACAAATACC AGCAGTTCCG TATGACTGAG GAGCCTGAGA AGGCACAGCT TCACTTAGAC 
CAAGTGCTCA CTAGTCCTTT CAACACGACA TTTCCACTAG ATTACATGAA AGATGAGTTC 
CTCCCAGCCC TGTCTTTGCA GAGTGGCGCT TTACATGGCA GTTCTGAGAG CCTGAAGCCC 
GAAGGGGTGG CAGATTCTCC GTGTGGCAGC CTGATGGAAG GGACCCCTCA GATTAGTTCA 
GAGGAAAGCT ATAAACATGA GGGCCTAGCC GAGACCCCCG AGACAAGCCC AGAGAGCCTT 
TCCTTCTCGC CAAAGAAAAG TGAGGAGCAG ATTGGGGAAA CACATGAAAC TACCAAGTTA 
GAAACAGCAC CAGAAAGTCA TTCCGAAAAA GGACATCCCT CAACCAAAGA TGTTGCTGAT 
GGTTCTGAAG GGCAAGGTGC TGCAGTCACC GAGGCAGTAG AGTCCTCTGC TGAGCATCAG 
CTGACAGAGG CCACCCCAGA CCCTTCCAAA GACATATGCC CCCCACAGGA AGGTGACAGC 
CTTGGTGGTC AAAGTGTATC ATTAGCAAAA GAAACATCCG AAGGACTGAC TGAGGAAGTG 
ACCTGTGACG AAGGTCCACG TGCACACATT AGTCCATCCC ACGAAACACA AACTGATACC 
GAAGCTCAGG AATCCACAGC CACCAAGCCC TCAGGGGAGG CAGAGGCCTC ACCGTTGTCC 
GAGGCTGCTG TAAAGGCAGG TTCAGGGACT GAATCAAAGC CCCAGGCAGC CATTAGAAGT 
CCGCAAGGGT TAGAACTCGC ACTCCCTAGC CGCGAGAGTG AGGTTTTCAG CCCTGGGGCT 
GATGAATCCT TCGCTGTGAG CCACAAAGAC TCCCTGGAGG CCAGCCCTGT GCTGGAAGAT 
AACTCTTCGC ACAAAACTCC CGATTCTCTG GAACCAAGTC CTCTGAAGGA ATCCCCTTGC 
CGTGACTCTC TTGAAAGCAG CCCCGTTGAA CCAAAGATGA AGGCTGGAAT TCTCCCAAGT 
CACTTTCCTC TTCCCTCAGC TGTTGCCAAG ACCGAGCTCT TCACGGAAGT GGCCTCTGTG 
CGGTCCCGGC TGCTCCGAGA CCCCGACGGC AGTGCCGAGG ATGACAGCCT CGAGCAGACG 
TCACTCATGG AGAGCTCGGG GAAGAGTCCC CTTTCCCCAG ACACCCCCAG CTCCGAAGAA 
GTTAGCTACG AGGTCACGCC CAAAACCACA GACACACCAA AACCAGCTGT GATTCCTGAA 
TGTGCGGAGG AGGACGACTC AGAAAATGGG GAGAAAAAGA GGTTCACACC GGAAGAGGAA 
ATGTTTAAAA TGGTAACCAA AGTCAAAACG TTTGATGAAC TTGAACAAGA AGCAAAGCAG 
AAAAGGGACT ACAAAAGAGA ACCCAAGCAG GAAGAATCTT CTTCATCCTC TGATGCAGAT 
GTCGACTGTT CAGCAGACAC GGATGAACCA AAACACACAG AGAGTGTCGA GGATGAAAGT 
GACGCTCCTG TGGTAGTAAC AACAGAGGGC AGGGAGGCTT CTTCCTCCTC AGAAAGTGAA 
CCTGAGTTGA CACAGCTGAA GAAAGGTGCT GACTCAGGTC TCTTGGCAGA GCCAGTTATT 
CGAGTGCAGC CTCCTTCCCC ACTTCCATCC AGCATAGACT CAAATTCCAG TCCTGAAGAA 
ATACAATTCC AGCCTATCGT TTCCAAACAG TACACTTTTA AGATGAATGA AGATGCTCAG 
GAAGATTCAG ATAAATCAGA AGAAGAAAAA GATTATAAAT CTCGCTTACC CGAAGACAGT 
CATGCTCTTC CCACTGATGG ACCAGATATG TCTTATGATG ATCTGAATGG AGATACTTAT 
CAATCAAAAC TTTGTGATGG CCTTGAGTAT GAGATTGGGA GTCCTAGCGA CTCAGCTGCT 
CCTGTCTCTT CAGGTCTCCG CAGTTCCCCT GGTGACGATG TCAGTGAGCA GCTAGTTATC 
CATAAGGAAT CACTAGCCCT CCAAGACACT GGTGAGAACG ACCTAGAAGA GCTTGATGTT 
CCTGAAGTCG AATCTTCACA AGCAGATCCC CCCAGTGAAG GCTCTCCATC TTTCTCCTCT 
TTACCTCTTT GTCCAGCATC TGAAGCAAAA GAATTAGATG AAGACATGGT CTCTACAGCT 
TCTGCTACAG AAATGGAGGT CACAAAACCT GACCAAGCTT TTGAGAGCTT ACCAGAGGAC 
TGTTCCATTC AAGATTCACT CATTACTACT CAAACTGATA GATCATCCTT GGATGTTCCA 
GTGTCAGACC CAACAGAGAC TGAGGAAATC TATGATCCTC AAATCATAAG CCCTTATGAA 
AATGTTCCTT CTCAATCTTT TTTCTCTGGT GAAGAAAGCA AAACCCAAAC GGATACAAGG 
CACACAAGTT TTCATTCTTC TGAAGAGTAT TCTGTTACCA TCACATCCTC TGTTGAAGAT 
GTAGTAGAGA CAAGCTCCTC TGATAAAACT GTTTCAAGTA AAGAAGCTAA TTTTGAGGAG 
CAGGTCTTGG AAGTAGAATC CAAACAAGAG AGTACCTTGC AGGAAATACA GTCACATGGA 
GCCTCCTCAT CTTTGGAGCC ACCTGTACCA AAAACATCAA CAGTTGTCAG TGAACAAATA 
AGCAAAGTCA TCATCACAAA AACTGATGTG GATTCTGATT CCTGGAGTGA AATCCGTGAG 
GATGACGAAG CCTTTGAGGC TCGAGTGAAA GAGGAAGAAC AGAAGATATT TGGTTTGATG 
GTAGACAGAC AGTCACAGGG TACCACCCCT GACACCACTC CTGCTAGGAC CCCAACTGAA 
GAGGGGACCC CAACCAGTGA GCAAAATCCA TTTCTCTTTC AGGAAGGAAA ATTGTTTGAG 
ATGACCCGAA GTGGTGCCAT TGATATGACC AAAAGGTCCT ATGCCGATGA AAGCTTTCAC 
TTTTTCCAAA TTGGGCAAGA ATCCAGGGAA GAGGCTGTCT CACAAGACAT GAAAGAAGGA 
GGTACAGGTG CTGAGGCCCC ACAGGTGGAG ACATCAGCTG AGTCATTAAC ACTCTCAGAA 
TCAAAAGAAA CAGTGGATGA TGAAGCAGAG TTACTCCCAG ATGACCTGAG TGAGGAGGTA 
GAAGAAATAC ACACTTCAGA TACCCCACTT AGCTCCCAAA TAGACATTTC AGCCTCCACA 
GAAACATCCA TGAAAGAGGC TACTTCTACA GGGCTTGAGG ACCTACCCAC CAGGCAAATG 
GGTGATTCTC TTCCTCCATC CAGCGAGAAG CAGACCACTT GCCCTGACTT CCCTGAACAA 
GTTGTACAAG TTCAGTTAGA TTTTTCCACA GTCACTAGGT CTGTATATTC AGATCGGGGT 
GATGATTCTC CCGATTCCTC CCCAGAGGAA CAGAAATCAG TCATTGAAAT CCCTACTGCA 
ACCATGGAGA ATGTGCCTTC TACTGAGAGC AAATCCAAAA TCCCTGTAAG GACTATCCCC 
ACTTCAACCT CAATACCTCC ATCGGTGGAA TATGAGAGTT CCCTTTCTGA AGATTTTCTG 
CCCAGCCTGG ATGAGGAAAG TAAAGAAGAT GAAGCAAAAC CAAAGTCTAA AATCCCCGTG 
AAAGCACCTG CCCAAAGAGT GGAGCAGCAG CCTTCACATC TAGACTCACC TCTAAAGAAG 
ACAGGTCGTC CCCAGGGACA GGACTTGACA AGCAGTGCAC CAGATGATAG AAGCAAATCT 
GAATCTGATG CCAGTTTGGA CGTGAAGACC AAATGTCCAG TAGAAACTAG AAGTTACACT 
GAGGCAGAAA TAGAAAGCAC AGGTGAGGAA GAGGAGCTTG AGTTAGAATC AGAGGAAGGG 
GCCACAAGAC CAAAGATATT TGCATCCCGA TTGCCAGTAA AGAGCAAAAG CACCGCATCT 
TCCTGCAGGG GGCCCACAAG CCCCACAAAA GACAATAAGG AGCATTTCTT TGATCTTTAC 
AGGAACTCCA TAGAATTCTT TGAGGAGATT AGTGATGAAG CTTCCAAGTT AGTGGATAGA 
CTTACACAGT CAGAAAGGGA GCAAGATTTA GTTTCAGATG ATGAAAGTAG TAGTGCCCTG 
GAAGTTTCAG TAATTGAAAA TCTGCCACCT GTTGAGACCG AGAACTCAAT TCCTGAGGAC 
CTCTTTGACA CAAGGCCCAT TTGGGATGAG TCCATTGAGA CTCTGATCGA ACGCATCCCT 
GATGAAAATG GCCATGACCA TGCTGAAGGT ATTTGCCAGC CACTGGGATT CCCTGTGCTA 
CGCATGTCAG TCACCAGGAC CAAGACAAAC GTTGTTTCTG GCCATACGGA AAAAACCGGG 
ATGGTCCGAT CAACCGAGTT GGCAAGAGAA CTGGACTTCA CTGAAGAGCA AATTCATCAA 
ATTCGAATTG AGAACCCTAA CTCCCTGCAG GATCAGAGTC ATGCGCTGTT GAAGTACTGG 
CTAGAAAGGG ACGGGAGACA TGCTACAGAT ACCAGTCTCG TTGAATGTCT CACCAAGATC 
AACCGAATGG ATATCGTGCA TCTCATGGAG ACCAGCACAG AAGCTCTCCA GGAGCGCATC 
GGTCATAGCT ATGCAGAGAT TGAGCAGACC ATTACACTGG ATCATAGTGA AGGGTTCTCA 
GTACTCCAAG AGGAGCTTTG CACTGCCCAG CACAAGCAGA AAGAGGAGCA TGCTGCTTCT 
AAAGAGGACG AGGCCTCTGA CCACCCTCCC ATCGTCTCAG AGGAAGACAT TTCTGTTGGT 
TACTCCACTC TTCAGGATGG CATCCCCAAA ACTGAGGGTG ATGGCTCTGC AACAGAGCTC 
CTTCCCCAGA CTCAAAAGGA GCAAGTTCAA CAGGATTTCT CAGGGAAAAT GCAAGACCTG 
CCTGAGGAGT CGGCTCTTGA ACAGCAAGAA TACTTCGTGA CAACCCCAGG GACAGAAATG 
TCAGAGACTC AGAAGGCCAC GGGAACATCG GGCTCCCCCA CCAAGACACC TGAGGAAATT 
GTCACCCCTT CCGAGGAGGA GAGGCTGTAT CTCCAAACCC CAACATCGAG TGAGCGGGGA 
GGCTCTCCAG TTGTGCAGGA ACCTGAGGAG CCCCCCGAAC GCAGGGAGGA GAGTTCACCT 
CGGAAAACCA GCCTCGTGAT CGTGGAATCG GCTGAGGACC AGCCTCAGGC CTTCGAGAGG 
CTTGATGAAG AGGCTGCTTT TCAAAAGGGA GATGATATGC CTGACATACC CCCAGAAACA 
GTCACAGAAG AAGAATACAT CGATGAGCAT GGACACACCG TGGTAAAGAA GGTTACTAGG 
AAAATCATTA GGCGGTTCAT CTCCTCTGAC GGCACAGAGA AAGAGGAGAT TACGATGCAG 
GGAGCACCAC AGGATTCTAT CAGCGTTGAT GAAGGGGATG GTTATTCCAA AGTCATAAAG 
CGCATTGTAT TGAAGAGTGA CACCGAGCAG TCAGAGGTAA CTTTGTCTGA GCCCAGCATT 
CTGTCCAGTA CCTCACAATT TCAGGCTGAA CCAGTGGAAG GCCGTAGAGT CAGCAAAGTT 
GTTAAGACAA CTGTGGTCCA TGGTGAGAGG ATGGAGAAAC ATCTGGGGGA TTCTAGTTTA 
GCCACTGATC TTCCTTCAGC CAAAGATGAC TTTGAAGAGG CTTTGAGTTA CACAGGTAGC 
CACATGAAAG TCTACTCCCC CAGTTTAGTA GAGAATGAAG TCCTGAAGGA GGATGGATCA 
ATAATTAGAA GGACGACAAT GAGTAAAGCT AGAACCCAGA AGAGGGCTGT GGTGAAGGAC 
CAGCAGGGAA AACGCATTTA CTTGGAGCAC CTGGAGGATG TCCCAGAAGC GCTAGACCAG 
GACGACCTCC AGCACGACCT CCAGCAACTC CTTCGGCATT TCTGCAAGGA GGACTCGAAG 
CAAGAGGCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANK2 ( XM_015463691.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319177.1 Bos taurus ankyrin-2 (LOC539238), transcript variant X2, mRNA.

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CloneID	OBa90060
Clone ID Related Accession (Same CDS sequence)	XM_015463691.1
Accession Version	XM_015463691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LOW QUALITY PROTEIN: ankyrin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGACTAACA GATTTAATGT TTCCAGTATT TTTCCCAAGT GGAGAATCAA CATAGTTTGC 
TTAATAGGTT TTGTTAAAAG GATTTTGTTA GCAGTCACAA AGACAAATGC TACATTTTAC 
TTTTGTTCTT CCCATTTTCA GAAGGGTTTT ACCCCCTTGC ACGTAGCTGC CAAGTATGGG 
AGCCTGGATG TGGCAAAACT TCTCTTGCAA CGCCGTGCTG CCGCAGATTC TGCGGGGAAG 
AATGGCCTTA CCCCGCTCCA TGTTGCTGCT CACTATGACA ACCAGAAGGT GGCGCTGCTG 
TTACTGGAGA AGGGTGCTTC CCCTCACGCC ATGGCCAAGA ATGGCTACAC TCCTTTACAT 
ATTGCTGCCA AGAAGAATCA AATGCAGATA GCTTCCACAC TCCTGAGCTA CGGAGCAGAG 
ACGAACATCG TGACAAAGCA AGGAGTCACT CCACTCCATC TGGCCTCGCA GGAGGGACAC 
ACGGATATGG TCACCTTGCT TCTGGATAAG GGAGCCAATA TCCACATGTC AACCAAGAGT 
GGACTCACAT CTTTACACCT TGCAGCCCAG GAAGATAAAG TGAATGTCGC TGATATTCTC 
ACCAAGCATG GGGCTGACCA GGATGCGCAC ACAAAGCTTG GTTACACTCC TTTAATTGTG 
GCCTGTCACT ATGGAAACGT GAAAATGGTC AACTTCCTTC TGAAGCAGGG AGCAAATGTC 
AATGCAAAAA CCAAGAATGG CTACACGCCT TTGNCCACCA GCGCCCAACA GGGCCACACG 
CACATCATCA ATGTTTTGCT GCAGCACGGG GCCAAGCCCA ACGCTACCAC CGCGAATGGC 
AACACTGCCT TGGCGATTGC TAAGCGTCTG GGCTACATCT CGGTGGTGGA CACCCTGAAG 
GTTGTAACGG AGGAGGTCAC CACCACCACC ACGACTATCA CTGAAAAACA CAAGCTAAAT 
GTTCCTGAGA CCATGACTGA GGTTCTTGAT GTTTCTGATG AAGAGGCTTT TAAACAGTTT 
GGTGACCACT TTATTGATGG GGAAGTTCTT AGTGATTCAG GTGATGACAC AATGACGGGT 
GATGGGGGAG AGTACCTTAG ACCTGAGGAC CTAAAGGAGC TGGGTGATGA CTCCCTCCCC 
AGCAGTCAGT TCCTGGACGG GATGAATTAC CTGCGCTACA GCTTGGAGGG GGGCCGCTCC 
GACAGTACCA TACCCAGTTC CGACAGGTCT CACACCCTGA GCCATGCCTC GTACCTGAGG 
GACAGTGCCA TGATCGACGA CGCAGTTGTG ATCCCCAGTC ACCAGGTGAC AACTCTAGCC 
AAGGAGGCAG AAAGGAATTC TTATCGTCTA AGCTGGGGCA CTGAGAACTT GGACAACGTG 
GCTCTTTCTT CCAGCCCTAT TCATTCAGGC CGCACCTCTC CATGTCTTGA TCGTGACAAC 
AGCAGTTTCC TGGTTAGTTT TATGGTGGAT GCCCGAGGTG GTGCTATGCG AGGATGCAGA 
CACAACGGGC TCCGCATCAT TATTCCGCCC CGGAAATGTA CCGCCCCAAC ACGAGTCACC 
TGCCGACTGG TCAAACGCCA TAGACTGGCG ACAATGCCTC CAATGGTGGA AGGAGAGGGC 
CTGGCCAGTC GCCTGATTGA AGTTGGACCT TCTGGTGCTC AGTTCCTTGG TAAACTTCAC 
CTGCCAACGG CTCCTCCCCC ACTTAATGAG GGAGAAAGTT TGGTCAGCCG CATCCTTCAG 
CTGGGGCCTC CTGGAACCAA ATTCCTTGGG CCTGTGATCG TGGAGATCCC TCATTTCGCT 
GCCCTGCGAG GAAAAGAGAG GGAACTGGTG GTCCTGCGCA GTGAGAACGG GGACAGCTGG 
AAAGAGCATT ACTGTGAATA CACGGAGGAG GAGCTGAATG AAATCCTGAA CGGCATGGAT 
GAAGTGCTGG ATAGCCCGGA AGACCTCGAA AAGAAGCGGA TCTGCCGCAT CATCACCCGT 
GACTTCCCAC AGTACTTTGC GGTGGTGTCG CGCATCAAGC AGGACAGCAA CCTGATTGGC 
CCGGAGGGAG GCGTGCTGAG CAGCACCGTG GTGCCGCAGG TGCAGGCCGT GTTCCCCGAG 
GGGGCGCTAA CCAAGCGGAT CCGTGTTGGC CTACAGGCTC AACCTATGCA CAGTGAGTTG 
GTTAAGAAGA TTCTGGGCAA CAAAGCTACC TTCAGCCCTA TAGTTACTTT GGAACCTAGA 
AGAAGAAAAT TTCACAAGCC AATTACCATG ACCATTCCTG TCCCCAAAGC TTCAAGTGAT 
GTCATGTTGA ATGGTTTTGG AGGAGATGCA CCAACCTTAA GATTACTATG TAGCATCACA 
GGTGGAACCA CCCCTGCTCA GTGGGAAGAT ATTACAGGAA CTACGCCATT AACATTTGTC 
AATGAATGTG TTTCCTTTAC AACCAATGTG TCTGCCAGGT TCTGGCTGAT CGATTGTCGA 
CAAATTCAGG AATCTGTTAC CTTTGCATCA CAAGTGTATA GAGAAATTAT CTGTGTACCA 
TATATGGCCA AATTTGTAGT GTTTGCCAAA TCACATGACC CCATTGAAGC CAGGTTGAGG 
TGTTTCTGCA TGACTGATGA TAAAGTGGAT AAAACCCTGG AACAACAAGA AAACTTTGCT 
GAGGTGGCCA GAAGCAGGGA TGTGGAGGTC TTAGAAGGAA AACCCATCTA TGTTGATTGT 
TTTGGCAACC TGGTGCCATT AACCAAGAGT GGCCAACATC ATATATTCAG TTTTTTTGCC 
TTCAAAGAAA ACAGACTTCC TCTCTTTGTC AAGGTACGTG ATACGACTCA GGAACCTTGC 
GGACGACTAT CATTTATGAA AGAGCCAAAA TCTACAAGAG GCCTGGTGCA CCAAGCTATT 
TGCAACTTAA ACATCACCCT GCCAATTTAT ACGAAGGAAT CAGAGTCAGA TCAAGAACAG 
GAGGAAGAGA TCGATATGAC ATCAGAAAAA AATGATGAGA CAGAATCTAC AGAAACATCT 
GTCCTGAAAA GTCACCTGGT TAATGAAGTT CCTGTCCTGG CAAGTCCGGA CTTGCTCTCT 
GAAGTTTCTG AGATGAAACA AGATTTGATC AAAATGACCG CCATCTTGAC CACAGATGGA 
TCTGATAGGG CAGGTTCCAT CAAAGTGAAG GAGCTGGTGA AGGCTGCAGA GGAAGAGCCA 
GGGGAGCCTT TTGAAATTGT TGAAAGAGTT AAAGAGGACT TAGAGAAGGT GAACGAAATC 
CTGAGAAGCG GAACCTGCAC AGGAGATGAA GGCAGTGTGC CGAGGTCTCA GCCAGAGAGA 
GAGTTAGTGG AGGAGGAGTG GGTCATTGTC AGTGAGGAGG AGATAGAGGA GGCTAAGCAA 
AAAGCATCTT TAGAGATCGC CGAATACCCA TGTGTAGAAG TTAGACTAGA TAAAGAGACC 
AAAGTAGAAG TAGAGAAAGA CTCAACGGGG CTAGTGAACT ACCTTACTGA GGATCTGAAT 
TCCTATGTGC CTCCTCACGA AGAGCAGCCC CAGAAGGAGC AAGGTATGGT AGGGGAGAAG 
CCTGAGGCTC AGGGTCCCAG CAGGCGCTCA GAAAGCGAAG GCAAAGATGC ACCCTCAGAG 
GAGACACAGA GTACACAGAA ACAGCCCAAA CCAAGCCTGG GGATCAAGAA GCCGGTGAGA 
AGGAAATTAA AGGAGAAACA AAAGCAGAAG GAGGACAGTC CACAAGCTAG TGCAGAAAAA 
ACAGAACTTA AAAAAGGGAG TTCAGAAGAG TCATTAGATG AAGACACAAG TTCAGCCCCC 
GAGCCTCTTC CTGCTGTAAA AGCTACGTCA CCTTTGATAG AGGAAACGCC CATTGGTTCC 
ATAAAGGACA AGGTGAAGGC CCTTCAGAAA CGAGTGGAAG ATGAACAGAA GGGTCGAAGC 
AAGTTGCCTG TCAGAGTCAA AGGCAAAGAG GATGTGCCAA AAAAGGTAAC ACAAAGGACA 
CATTTGACTG CATCGCCCTC TCTGAAGTCA GAGAGGCATG CGCCAGCGTC CGCCTCCTCT 
AGAACAGAAA GACACACTTC TCTTTCCTCC TCTGCCAAAA CAGAAAGGCA CCCTCCGGTA 
TCACCATCGA GTAAGAACGA GAAACACTCG CCTGTGTCAC CCTCCGCGAA AGCTGAAAAG 
CAGTCCCCTG TGTCATCCTC AAGCAAAACT GAGAAACACT CACCCGTTTC ACCCTCGACC 
AAAACGGAAA GACACTCCCC TATGTCCTCT TCTACAAAAA CAGAAAGACA CCCTCCTGTT 
TCGCCTTCAG GTAAGACAGA TAAACGTCCC CCTGGATCTC CCTCTGGGAG ATCCGAGAAA 
CATCCGCCGG TATCTCCTGG CAGAACAGAG AAACGCTTGC CGGTGTCACC CGCTGGAAGG 
ATGGAAAAGC ATTCTCCTGT CTCAAGCTCC GGGAAAACTG AGAAGCACCT GCCCGTGTCA 
CCTTCTGGGA AAACAGACAA ACAACCACCT GTCTCCCCCA CCTCAAAAAC AGAAAGAATC 
GAGGAGACCA TGTCTGTTCG GGAGCTGATG AAAGCTTTCC AGTCAGGTCA GGACCCATCG 
AAACACAAGA CTGGACTTTT TGAGCACAAA TCTGCAAAAC CAAAACAGCC ACAAGAAAAG 
GGTAGAGGTC GGCTAGAAAA AGAAAAGGGG CCGATAGTAA CCCCAAGAGA AGCGCAGAAA 
ACGGAGAGTC AGACAATCAA ACGAGGCCAG AGGTTCCTGG TCACGGGATT CTCAGAACCC 
AGAAGAGCAG TCCGTGCTTC CTCCACAGGG TTTAAGAGAG AAGATGGGGC TGGAGAAAAG 
GAGAAATCTC TCAACCACAA AACACCCGAA CCTGTTCAGC CAGTGCCTGA AGAAGAAAGC 
CATAAAGATA GCGAAGTCCC CAAAGAAAAG CTGGCTGACG ACCAGGGAGA CATGGATCTC 
CAGATCAGCC CAGACCGGAA AACCTCCACT GACTTTTCTG ACGTCATTAA GCAAGAACTG 
GAAGACAATG ACAAATACCA GCAGTTCCGT ATGACTGAGG AGCCTGAGAA GGCACAGCTT 
CACTTAGACC AAGTGCTCAC TAGTCCTTTC AACACGACAT TTCCACTAGA TTACATGAAA 
GATGAGTTCC TCCCAGCCCT GTCTTTGCAG AGTGGCGCTT TACATGGCAG TTCTGAGAGC 
CTGAAGCCCG AAGGGGTGGC AGATTCTCCG TGTGGCAGCC TGATGGAAGG GACCCCTCAG 
ATTAGTTCAG AGGAAAGCTA TAAACATGAG GGCCTAGCCG AGACCCCCGA GACAAGCCCA 
GAGAGCCTTT CCTTCTCGCC AAAGAAAAGT GAGGAGCAGA TTGGGGAAAC ACATGAAACT 
ACCAAGTTAG AAACAGCACC AGAAAGTCAT TCCGAAAAAG GACATCCCTC AACCAAAGAT 
GTTGCTGATG GTTCTGAAGG GCAAGGTGCT GCAGTCACCG AGGCAGTAGA GTCCTCTGCT 
GAGCATCAGC TGACAGAGGC CACCCCAGAC CCTTCCAAAG ACATATGCCC CCCACAGGAA 
GGTGACAGCC TTGGTGGTCA AAGTGTATCA TTAGCAAAAG AAACATCCGA AGGACTGACT 
GAGGAAGTGA CCTGTGACGA AGGTCCACGT GCACACATTA GTCCATCCCA CGAAACACAA 
ACTGATACCG AAGCTCAGGA ATCCACAGCC ACCAAGCCCT CAGGGGAGGC AGAGGCCTCA 
CCGTTGTCCG AGGCTGCTGT AAAGGCAGGT TCAGGGACTG AATCAAAGCC CCAGGCAGCC 
ATTAGAAGTC CGCAAGGGTT AGAACTCGCA CTCCCTAGCC GCGAGAGTGA GGTTTTCAGC 
CCTGGGGCTG ATGAATCCTT CGCTGTGAGC CACAAAGACT CCCTGGAGGC CAGCCCTGTG 
CTGGAAGATA ACTCTTCGCA CAAAACTCCC GATTCTCTGG AACCAAGTCC TCTGAAGGAA 
TCCCCTTGCC GTGACTCTCT TGAAAGCAGC CCCGTTGAAC CAAAGATGAA GGCTGGAATT 
CTCCCAAGTC ACTTTCCTCT TCCCTCAGCT GTTGCCAAGA CCGAGCTCTT CACGGAAGTG 
GCCTCTGTGC GGTCCCGGCT GCTCCGAGAC CCCGACGGCA GTGCCGAGGA TGACAGCCTC 
GAGCAGACGT CACTCATGGA GAGCTCGGGG AAGAGTCCCC TTTCCCCAGA CACCCCCAGC 
TCCGAAGAAG TTAGCTACGA GGTCACGCCC AAAACCACAG ACACACCAAA ACCAGCTGTG 
ATTCCTGAAT GTGCGGAGGA GGACGACTCA GAAAATGGGG AGAAAAAGAG GTTCACACCG 
GAAGAGGAAA TGTTTAAAAT GGTAACCAAA GTCAAAACGT TTGATGAACT TGAACAAGAA 
GCAAAGCAGA AAAGGGACTA CAAAAGAGAA CCCAAGCAGG AAGAATCTTC TTCATCCTCT 
GATGCAGATG TCGACTGTTC AGCAGACACG GATGAACCAA AACACACAGA GAGTGTCGAG 
GATGAAAGTG ACGCTCCTGT GGTAGTAACA ACAGAGGGCA GGGAGGCTTC TTCCTCCTCA 
GAAAGTGAAC CTGAGTTGAC ACAGCTGAAG AAAGGTGCTG ACTCAGGTCT CTTGGCAGAG 
CCAGTTATTC GAGTGCAGCC TCCTTCCCCA CTTCCATCCA GCATAGACTC AAATTCCAGT 
CCTGAAGAAA TACAATTCCA GCCTATCGTT TCCAAACAGT ACACTTTTAA GATGAATGAA 
GATGCTCAGG AAGATTCAGA TAAATCAGAA GAAGAAAAAG ATTATAAATC TCGCTTACCC 
GAAGACAGTC ATGCTCTTCC CACTGATGGA CCAGATATGT CTTATGATGA TCTGAATGGA 
GATACTTATC AATCAAAACT TTGTGATGGC CTTGAGTATG AGATTGGGAG TCCTAGCGAC 
TCAGCTGCTC CTGTCTCTTC AGGTCTCCGC AGTTCCCCTG GTGACGATGT CAGTGAGCAG 
CTAGTTATCC ATAAGGAATC ACTAGCCCTC CAAGACACTG GTGAGAACGA CCTAGAAGAG 
CTTGATGTTC CTGAAGTCGA ATCTTCACAA GCAGATCCCC CCAGTGAAGG CTCTCCATCT 
TTCTCCTCTT TACCTCTTTG TCCAGCATCT GAAGCAAAAG AATTAGATGA AGACATGGTC 
TCTACAGCTT CTGCTACAGA AATGGAGGTC ACAAAACCTG ACCAAGCTTT TGAGAGCTTA 
CCAGAGGACT GTTCCATTCA AGATTCACTC ATTACTACTC AAACTGATAG ATCATCCTTG 
GATGTTCCAG TGTCAGACCC AACAGAGACT GAGGAAATCT ATGATCCTCA AATCATAAGC 
CCTTATGAAA ATGTTCCTTC TCAATCTTTT TTCTCTGGTG AAGAAAGCAA AACCCAAACG 
GATACAAGGC ACACAAGTTT TCATTCTTCT GAAGAGTATT CTGTTACCAT CACATCCTCT 
GTTGAAGATG TAGTAGAGAC AAGCTCCTCT GATAAAACTG TTTCAAGTAA AGAAGCTAAT 
TTTGAGGAGC AGGTCTTGGA AGTAGAATCC AAACAAGAGA GTACCTTGCA GGAAATACAG 
TCACATGGAG CCTCCTCATC TTTGGAGCCA CCTGTACCAA AAACATCAAC AGTTGTCAGT 
GAACAAATAA GCAAAGTCAT CATCACAAAA ACTGATGTGG ATTCTGATTC CTGGAGTGAA 
ATCCGTGAGG ATGACGAAGC CTTTGAGGCT CGAGTGAAAG AGGAAGAACA GAAGATATTT 
GGTTTGATGG TAGACAGACA GTCACAGGGT ACCACCCCTG ACACCACTCC TGCTAGGACC 
CCAACTGAAG AGGGGACCCC AACCAGTGAG CAAAATCCAT TTCTCTTTCA GGAAGGAAAA 
TTGTTTGAGA TGACCCGAAG TGGTGCCATT GATATGACCA AAAGGTCCTA TGCCGATGAA 
AGCTTTCACT TTTTCCAAAT TGGGCAAGAA TCCAGGGAAG AGGCTGTCTC ACAAGACATG 
AAAGAAGGAG GTACAGGTGC TGAGGCCCCA CAGGTGGAGA CATCAGCTGA GTCATTAACA 
CTCTCAGAAT CAAAAGAAAC AGTGGATGAT GAAGCAGAGT TACTCCCAGA TGACCTGAGT 
GAGGAGGTAG AAGAAATACA CACTTCAGAT ACCCCACTTA GCTCCCAAAT AGACATTTCA 
GCCTCCACAG AAACATCCAT GAAAGAGGCT ACTTCTACAG GGCTTGAGGA CCTACCCACC 
AGGCAAATGG GTGATTCTCT TCCTCCATCC AGCGAGAAGC AGACCACTTG CCCTGACTTC 
CCTGAACAAG TTGTACAAGT TCAGTTAGAT TTTTCCACAG TCACTAGGTC TGTATATTCA 
GATCGGGGTG ATGATTCTCC CGATTCCTCC CCAGAGGAAC AGAAATCAGT CATTGAAATC 
CCTACTGCAA CCATGGAGAA TGTGCCTTCT ACTGAGAGCA AATCCAAAAT CCCTGTAAGG 
ACTATCCCCA CTTCAACCTC AATACCTCCA TCGGTGGAAT ATGAGAGTTC CCTTTCTGAA 
GATTTTCTGC CCAGCCTGGA TGAGGAAAGT AAAGAAGATG AAGCAAAACC AAAGTCTAAA 
ATCCCCGTGA AAGCACCTGC CCAAAGAGTG GAGCAGCAGC CTTCACATCT AGACTCACCT 
CTAAAGAAGA CAGGTCGTCC CCAGGGACAG GACTTGACAA GCAGTGCACC AGATGATAGA 
AGCAAATCTG AATCTGATGC CAGTTTGGAC GTGAAGACCA AATGTCCAGT AGAAACTAGA 
AGTTACACTG AGGCAGAAAT AGAAAGCACA GGTGAGGAAG AGGAGCTTGA GTTAGAATCA 
GAGGAAGGGG CCACAAGACC AAAGATATTT GCATCCCGAT TGCCAGTAAA GAGCAAAAGC 
ACCGCATCTT CCTGCAGGGG GCCCACAAGC CCCACAAAAG ACAATAAGGA GCATTTCTTT 
GATCTTTACA GGAACTCCAT AGAATTCTTT GAGGAGATTA GTGATGAAGC TTCCAAGTTA 
GTGGATAGAC TTACACAGTC AGAAAGGGAG CAAGATTTAG TTTCAGATGA TGAAAGTAGT 
AGTGCCCTGG AAGTTTCAGT AATTGAAAAT CTGCCACCTG TTGAGACCGA GAACTCAATT 
CCTGAGGACC TCTTTGACAC AAGGCCCATT TGGGATGAGT CCATTGAGAC TCTGATCGAA 
CGCATCCCTG ATGAAAATGG CCATGACCAT GCTGAAGATC AACAGGGTGA GCAGGAACGG 
ATTGAGGAAA GGCTGGCATA TATTGCTGAT CACCTTGGCT TCAGCTGGAC AGAGTTGGCA 
AGAGAACTGG ACTTCACTGA AGAGCAAATT CATCAAATTC GAATTGAGAA CCCTAACTCC 
CTGCAGGATC AGAGTCATGC GCTGTTGAAG TACTGGCTAG AAAGGGACGG GAGACATGCT 
ACAGATACCA GTCTCGTTGA ATGTCTCACC AAGATCAACC GAATGGATAT CGTGCATCTC 
ATGGAGACCA GCACAGAAGC TCTCCAGGAG CGCATCGGTC ATAGCTATGC AGAGATTGAG 
CAGACCATTA CACTGGATCA TAGTGAAGGG TTCTCAGTAC TCCAAGAGGA GCTTTGCACT 
GCCCAGCACA AGCAGAAAGA GGAGCATGCT GCTTCTAAAG AGGACGAGGC CTCTGACCAC 
CCTCCCATCG TCTCAGAGGA AGACATTTCT GTTGGTTACT CCACTCTTCA GGATGGCATC 
CCCAAAACTG AGGGTGATGG CTCTGCAACA GAGCTCCTTC CCCAGACTCA AAAGGAGCAA 
GTTCAACAGG ATTTCTCAGG GAAAATGCAA GACCTGCCTG AGGAGTCGGC TCTTGAACAG 
CAAGAATACT TCGTGACAAC CCCAGGGACA GAAATGTCAG AGACTCAGAA GGCCACGGGA 
ACATCGGGCT CCCCCACCAA GACACCTGAG GAAATTGTCA CCCCTTCCGA GGAGGAGAGG 
CTGTATCTCC AAACCCCAAC ATCGAGTGAG CGGGGAGGCT CTCCAGTTGT GCAGGAACCT 
GAGGAGCCCC CCGAACGCAG GGAGGAGAGT TCACCTCGGA AAACCAGCCT CGTGATCGTG 
GAATCGGCTG AGGACCAGCC TCAGGCCTTC GAGAGGCTTG ATGAAGAGGC TGCTTTTCAA 
AAGGAGCTAA CAGAGGAATT AGGTGAGCTG GAGGCCAGCT CAGATGAGGA GGCGATGGTA 
ACAACCAGGG TTGTCCGCCG ACGATTGATT ATTCAGGGAG ATGATATGCC TGACATACCC 
CCAGAAACAG TCACAGAAGA AGAATACATC GATGAGCATG GACACACCGT GGTAAAGAAG 
GTTACTAGGA AAATCATTAG GCGGTTCATC TCCTCTGACG GCACAGAGAA AGAGGAGATT 
ACGATGCAGG GAGCACCACA GGATTCTATC AGCGTTGATG AAGGGGATGG TTATTCCAAA 
GTCATAAAGC GCATTGTATT GAAGAGTGAC ACCGAGCAGT CAGAGGTAAC TTTGTCTGAG 
CCCAGCATTC TGTCCAGTAC CTCACAATTT CAGGCTGAAC CAGTGGAAGG CCGTAGAGTC 
AGCAAAGTTG TTAAGACAAC TGTGGTCCAT GGTGAGAGGA TGGAGAAACA TCTGGGGGAT 
TCTAGTTTAG CCACTGATCT TCCTTCAGCC AAAGATGACT TTGAAGAGGC TTTGAGTTAC 
ACAGGTAGCC ACATGAAAGT CTACTCCCCC AGTTTAGTAG AGAATGAAGT CCTGAAGGAG 
GATGGATCAA TAATTAGAAG GACGACAATG AGTAAAGCTA GAACCCAGAA GAGGGCTGTG 
GTGAAGGACC AGCAGGGAAA ACGCATTTAC TTGGAGCACC TGGAGGATGT CCCAGAAGCG 
CTAGACCAGG ACGACCTCCA GCACGACCTC CAGCAACTCC TTCGGCATTT CTGCAAGGAG 
GACTCGAAGC AAGAGGCCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319177.1
CDS	16..10959
Translation	MTNRFNVSSIFPKWRINIVCLIGFVKRILLAVTKTNATFYFCSSHFQKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHAMAKNGYTPLHIAAKKNQMQIASTLLSYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLnullTSAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTLKVVTEEVTTTTTTITEKHKLNVPETMTEVLDVSDEEAFKQFGDHFIDGEVLSDSGDDTMTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSTIPSSDRSHTLSHASYLRDSAMIDDAVVIPSHQVTTLAKEAERNSYRLSWGTENLDNVALSSSPIHSGRTSPCLDRDNSSFLVSFMVDARGGAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGKLHLPTAPPPLNEGESLVSRILQLGPPGTKFLGPVIVEIPHFAALRGKERELVVLRSENGDSWKEHYCEYTEEELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYFAVVSRIKQDSNLIGPEGGVLSSTVVPQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVTLEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSFTTNVSARFWLIDCRQIQESVTFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQENFAEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFMKEPKSTRGLVHQAICNLNITLPIYTKESESDQEQEEEIDMTSEKNDETESTETSVLKSHLVNEVPVLASPDLLSEVSEMKQDLIKMTAILTTDGSDRAGSIKVKELVKAAEEEPGEPFEIVERVKEDLEKVNEILRSGTCTGDEGSVPRSQPERELVEEEWVIVSEEEIEEAKQKASLEIAEYPCVEVRLDKETKVEVEKDSTGLVNYLTEDLNSYVPPHEEQPQKEQGMVGEKPEAQGPSRRSESEGKDAPSEETQSTQKQPKPSLGIKKPVRRKLKEKQKQKEDSPQASAEKTELKKGSSEESLDEDTSSAPEPLPAVKATSPLIEETPIGSIKDKVKALQKRVEDEQKGRSKLPVRVKGKEDVPKKVTQRTHLTASPSLKSERHAPASASSRTERHTSLSSSAKTERHPPVSPSSKNEKHSPVSPSAKAEKQSPVSSSSKTEKHSPVSPSTKTERHSPMSSSTKTERHPPVSPSGKTDKRPPGSPSGRSEKHPPVSPGRTEKRLPVSPAGRMEKHSPVSSSGKTEKHLPVSPSGKTDKQPPVSPTSKTERIEETMSVRELMKAFQSGQDPSKHKTGLFEHKSAKPKQPQEKGRGRLEKEKGPIVTPREAQKTESQTIKRGQRFLVTGFSEPRRAVRASSTGFKREDGAGEKEKSLNHKTPEPVQPVPEEESHKDSEVPKEKLADDQGDMDLQISPDRKTSTDFSDVIKQELEDNDKYQQFRMTEEPEKAQLHLDQVLTSPFNTTFPLDYMKDEFLPALSLQSGALHGSSESLKPEGVADSPCGSLMEGTPQISSEESYKHEGLAETPETSPESLSFSPKKSEEQIGETHETTKLETAPESHSEKGHPSTKDVADGSEGQGAAVTEAVESSAEHQLTEATPDPSKDICPPQEGDSLGGQSVSLAKETSEGLTEEVTCDEGPRAHISPSHETQTDTEAQESTATKPSGEAEASPLSEAAVKAGSGTESKPQAAIRSPQGLELALPSRESEVFSPGADESFAVSHKDSLEASPVLEDNSSHKTPDSLEPSPLKESPCRDSLESSPVEPKMKAGILPSHFPLPSAVAKTELFTEVASVRSRLLRDPDGSAEDDSLEQTSLMESSGKSPLSPDTPSSEEVSYEVTPKTTDTPKPAVIPECAEEDDSENGEKKRFTPEEEMFKMVTKVKTFDELEQEAKQKRDYKREPKQEESSSSSDADVDCSADTDEPKHTESVEDESDAPVVVTTEGREASSSSESEPELTQLKKGADSGLLAEPVIRVQPPSPLPSSIDSNSSPEEIQFQPIVSKQYTFKMNEDAQEDSDKSEEEKDYKSRLPEDSHALPTDGPDMSYDDLNGDTYQSKLCDGLEYEIGSPSDSAAPVSSGLRSSPGDDVSEQLVIHKESLALQDTGENDLEELDVPEVESSQADPPSEGSPSFSSLPLCPASEAKELDEDMVSTASATEMEVTKPDQAFESLPEDCSIQDSLITTQTDRSSLDVPVSDPTETEEIYDPQIISPYENVPSQSFFSGEESKTQTDTRHTSFHSSEEYSVTITSSVEDVVETSSSDKTVSSKEANFEEQVLEVESKQESTLQEIQSHGASSSLEPPVPKTSTVVSEQISKVIITKTDVDSDSWSEIREDDEAFEARVKEEEQKIFGLMVDRQSQGTTPDTTPARTPTEEGTPTSEQNPFLFQEGKLFEMTRSGAIDMTKRSYADESFHFFQIGQESREEAVSQDMKEGGTGAEAPQVETSAESLTLSESKETVDDEAELLPDDLSEEVEEIHTSDTPLSSQIDISASTETSMKEATSTGLEDLPTRQMGDSLPPSSEKQTTCPDFPEQVVQVQLDFSTVTRSVYSDRGDDSPDSSPEEQKSVIEIPTATMENVPSTESKSKIPVRTIPTSTSIPPSVEYESSLSEDFLPSLDEESKEDEAKPKSKIPVKAPAQRVEQQPSHLDSPLKKTGRPQGQDLTSSAPDDRSKSESDASLDVKTKCPVETRSYTEAEIESTGEEEELELESEEGATRPKIFASRLPVKSKSTASSCRGPTSPTKDNKEHFFDLYRNSIEFFEEISDEASKLVDRLTQSEREQDLVSDDESSSALEVSVIENLPPVETENSIPEDLFDTRPIWDESIETLIERIPDENGHDHAEDQQGEQERIEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGRHATDTSLVECLTKINRMDIVHLMETSTEALQERIGHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEHAASKEDEASDHPPIVSEEDISVGYSTLQDGIPKTEGDGSATELLPQTQKEQVQQDFSGKMQDLPEESALEQQEYFVTTPGTEMSETQKATGTSGSPTKTPEEIVTPSEEERLYLQTPTSSERGGSPVVQEPEEPPERREESSPRKTSLVIVESAEDQPQAFERLDEEAAFQKELTEELGELEASSDEEAMVTTRVVRRRLIIQGDDMPDIPPETVTEEEYIDEHGHTVVKKVTRKIIRRFISSDGTEKEEITMQGAPQDSISVDEGDGYSKVIKRIVLKSDTEQSEVTLSEPSILSSTSQFQAEPVEGRRVSKVVKTTVVHGERMEKHLGDSSLATDLPSAKDDFEEALSYTGSHMKVYSPSLVENEVLKEDGSIIRRTTMSKARTQKRAVVKDQQGKRIYLEHLEDVPEALDQDDLQHDLQQLLRHFCKEDSKQEAQ

Target ORF information:

RefSeq Version	XM_015463691.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin-2 (LOC539238), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463691.1

ORF Insert Sequence:

ATGACTAACA GATTTAATGT TTCCAGTATT TTTCCCAAGT GGAGAATCAA CATAGTTTGC 
TTAATAGGTT TTGTTAAAAG GATTTTGTTA GCAGTCACAA AGACAAATGC TACATTTTAC 
TTTTGTTCTT CCCATTTTCA GAAGGGTTTT ACCCCCTTGC ACGTAGCTGC CAAGTATGGG 
AGCCTGGATG TGGCAAAACT TCTCTTGCAA CGCCGTGCTG CCGCAGATTC TGCGGGGAAG 
AATGGCCTTA CCCCGCTCCA TGTTGCTGCT CACTATGACA ACCAGAAGGT GGCGCTGCTG 
TTACTGGAGA AGGGTGCTTC CCCTCACGCC ATGGCCAAGA ATGGCTACAC TCCTTTACAT 
ATTGCTGCCA AGAAGAATCA AATGCAGATA GCTTCCACAC TCCTGAGCTA CGGAGCAGAG 
ACGAACATCG TGACAAAGCA AGGAGTCACT CCACTCCATC TGGCCTCGCA GGAGGGACAC 
ACGGATATGG TCACCTTGCT TCTGGATAAG GGAGCCAATA TCCACATGTC AACCAAGAGT 
GGACTCACAT CTTTACACCT TGCAGCCCAG GAAGATAAAG TGAATGTCGC TGATATTCTC 
ACCAAGCATG GGGCTGACCA GGATGCGCAC ACAAAGCTTG GTTACACTCC TTTAATTGTG 
GCCTGTCACT ATGGAAACGT GAAAATGGTC AACTTCCTTC TGAAGCAGGG AGCAAATGTC 
AATGCAAAAA CCAAGAATGG CTACACGCCT TTGNCCACCA GCGCCCAACA GGGCCACACG 
CACATCATCA ATGTTTTGCT GCAGCACGGG GCCAAGCCCA ACGCTACCAC CGCGAATGGC 
AACACTGCCT TGGCGATTGC TAAGCGTCTG GGCTACATCT CGGTGGTGGA CACCCTGAAG 
GTTGTAACGG AGGAGGTCAC CACCACCACC ACGACTATCA CTGAAAAACA CAAGCTAAAT 
GTTCCTGAGA CCATGACTGA GGTTCTTGAT GTTTCTGATG AAGAGGCTTT TAAACAGTTT 
GGTGACCACT TTATTGATGG GGAAGTTCTT AGTGATTCAG GTGATGACAC AATGACGGGT 
GATGGGGGAG AGTACCTTAG ACCTGAGGAC CTAAAGGAGC TGGGTGATGA CTCCCTCCCC 
AGCAGTCAGT TCCTGGACGG GATGAATTAC CTGCGCTACA GCTTGGAGGG GGGCCGCTCC 
GACAGTACCA TACCCAGTTC CGACAGGTCT CACACCCTGA GCCATGCCTC GTACCTGAGG 
GACAGTGCCA TGATCGACGA CGCAGTTGTG ATCCCCAGTC ACCAGGTGAC AACTCTAGCC 
AAGGAGGCAG AAAGGAATTC TTATCGTCTA AGCTGGGGCA CTGAGAACTT GGACAACGTG 
GCTCTTTCTT CCAGCCCTAT TCATTCAGGC CGCACCTCTC CATGTCTTGA TCGTGACAAC 
AGCAGTTTCC TGGTTAGTTT TATGGTGGAT GCCCGAGGTG GTGCTATGCG AGGATGCAGA 
CACAACGGGC TCCGCATCAT TATTCCGCCC CGGAAATGTA CCGCCCCAAC ACGAGTCACC 
TGCCGACTGG TCAAACGCCA TAGACTGGCG ACAATGCCTC CAATGGTGGA AGGAGAGGGC 
CTGGCCAGTC GCCTGATTGA AGTTGGACCT TCTGGTGCTC AGTTCCTTGG TAAACTTCAC 
CTGCCAACGG CTCCTCCCCC ACTTAATGAG GGAGAAAGTT TGGTCAGCCG CATCCTTCAG 
CTGGGGCCTC CTGGAACCAA ATTCCTTGGG CCTGTGATCG TGGAGATCCC TCATTTCGCT 
GCCCTGCGAG GAAAAGAGAG GGAACTGGTG GTCCTGCGCA GTGAGAACGG GGACAGCTGG 
AAAGAGCATT ACTGTGAATA CACGGAGGAG GAGCTGAATG AAATCCTGAA CGGCATGGAT 
GAAGTGCTGG ATAGCCCGGA AGACCTCGAA AAGAAGCGGA TCTGCCGCAT CATCACCCGT 
GACTTCCCAC AGTACTTTGC GGTGGTGTCG CGCATCAAGC AGGACAGCAA CCTGATTGGC 
CCGGAGGGAG GCGTGCTGAG CAGCACCGTG GTGCCGCAGG TGCAGGCCGT GTTCCCCGAG 
GGGGCGCTAA CCAAGCGGAT CCGTGTTGGC CTACAGGCTC AACCTATGCA CAGTGAGTTG 
GTTAAGAAGA TTCTGGGCAA CAAAGCTACC TTCAGCCCTA TAGTTACTTT GGAACCTAGA 
AGAAGAAAAT TTCACAAGCC AATTACCATG ACCATTCCTG TCCCCAAAGC TTCAAGTGAT 
GTCATGTTGA ATGGTTTTGG AGGAGATGCA CCAACCTTAA GATTACTATG TAGCATCACA 
GGTGGAACCA CCCCTGCTCA GTGGGAAGAT ATTACAGGAA CTACGCCATT AACATTTGTC 
AATGAATGTG TTTCCTTTAC AACCAATGTG TCTGCCAGGT TCTGGCTGAT CGATTGTCGA 
CAAATTCAGG AATCTGTTAC CTTTGCATCA CAAGTGTATA GAGAAATTAT CTGTGTACCA 
TATATGGCCA AATTTGTAGT GTTTGCCAAA TCACATGACC CCATTGAAGC CAGGTTGAGG 
TGTTTCTGCA TGACTGATGA TAAAGTGGAT AAAACCCTGG AACAACAAGA AAACTTTGCT 
GAGGTGGCCA GAAGCAGGGA TGTGGAGGTC TTAGAAGGAA AACCCATCTA TGTTGATTGT 
TTTGGCAACC TGGTGCCATT AACCAAGAGT GGCCAACATC ATATATTCAG TTTTTTTGCC 
TTCAAAGAAA ACAGACTTCC TCTCTTTGTC AAGGTACGTG ATACGACTCA GGAACCTTGC 
GGACGACTAT CATTTATGAA AGAGCCAAAA TCTACAAGAG GCCTGGTGCA CCAAGCTATT 
TGCAACTTAA ACATCACCCT GCCAATTTAT ACGAAGGAAT CAGAGTCAGA TCAAGAACAG 
GAGGAAGAGA TCGATATGAC ATCAGAAAAA AATGATGAGA CAGAATCTAC AGAAACATCT 
GTCCTGAAAA GTCACCTGGT TAATGAAGTT CCTGTCCTGG CAAGTCCGGA CTTGCTCTCT 
GAAGTTTCTG AGATGAAACA AGATTTGATC AAAATGACCG CCATCTTGAC CACAGATGGA 
TCTGATAGGG CAGGTTCCAT CAAAGTGAAG GAGCTGGTGA AGGCTGCAGA GGAAGAGCCA 
GGGGAGCCTT TTGAAATTGT TGAAAGAGTT AAAGAGGACT TAGAGAAGGT GAACGAAATC 
CTGAGAAGCG GAACCTGCAC AGGAGATGAA GGCAGTGTGC CGAGGTCTCA GCCAGAGAGA 
GAGTTAGTGG AGGAGGAGTG GGTCATTGTC AGTGAGGAGG AGATAGAGGA GGCTAAGCAA 
AAAGCATCTT TAGAGATCGC CGAATACCCA TGTGTAGAAG TTAGACTAGA TAAAGAGACC 
AAAGTAGAAG TAGAGAAAGA CTCAACGGGG CTAGTGAACT ACCTTACTGA GGATCTGAAT 
TCCTATGTGC CTCCTCACGA AGAGCAGCCC CAGAAGGAGC AAGGTATGGT AGGGGAGAAG 
CCTGAGGCTC AGGGTCCCAG CAGGCGCTCA GAAAGCGAAG GCAAAGATGC ACCCTCAGAG 
GAGACACAGA GTACACAGAA ACAGCCCAAA CCAAGCCTGG GGATCAAGAA GCCGGTGAGA 
AGGAAATTAA AGGAGAAACA AAAGCAGAAG GAGGACAGTC CACAAGCTAG TGCAGAAAAA 
ACAGAACTTA AAAAAGGGAG TTCAGAAGAG TCATTAGATG AAGACACAAG TTCAGCCCCC 
GAGCCTCTTC CTGCTGTAAA AGCTACGTCA CCTTTGATAG AGGAAACGCC CATTGGTTCC 
ATAAAGGACA AGGTGAAGGC CCTTCAGAAA CGAGTGGAAG ATGAACAGAA GGGTCGAAGC 
AAGTTGCCTG TCAGAGTCAA AGGCAAAGAG GATGTGCCAA AAAAGGTAAC ACAAAGGACA 
CATTTGACTG CATCGCCCTC TCTGAAGTCA GAGAGGCATG CGCCAGCGTC CGCCTCCTCT 
AGAACAGAAA GACACACTTC TCTTTCCTCC TCTGCCAAAA CAGAAAGGCA CCCTCCGGTA 
TCACCATCGA GTAAGAACGA GAAACACTCG CCTGTGTCAC CCTCCGCGAA AGCTGAAAAG 
CAGTCCCCTG TGTCATCCTC AAGCAAAACT GAGAAACACT CACCCGTTTC ACCCTCGACC 
AAAACGGAAA GACACTCCCC TATGTCCTCT TCTACAAAAA CAGAAAGACA CCCTCCTGTT 
TCGCCTTCAG GTAAGACAGA TAAACGTCCC CCTGGATCTC CCTCTGGGAG ATCCGAGAAA 
CATCCGCCGG TATCTCCTGG CAGAACAGAG AAACGCTTGC CGGTGTCACC CGCTGGAAGG 
ATGGAAAAGC ATTCTCCTGT CTCAAGCTCC GGGAAAACTG AGAAGCACCT GCCCGTGTCA 
CCTTCTGGGA AAACAGACAA ACAACCACCT GTCTCCCCCA CCTCAAAAAC AGAAAGAATC 
GAGGAGACCA TGTCTGTTCG GGAGCTGATG AAAGCTTTCC AGTCAGGTCA GGACCCATCG 
AAACACAAGA CTGGACTTTT TGAGCACAAA TCTGCAAAAC CAAAACAGCC ACAAGAAAAG 
GGTAGAGGTC GGCTAGAAAA AGAAAAGGGG CCGATAGTAA CCCCAAGAGA AGCGCAGAAA 
ACGGAGAGTC AGACAATCAA ACGAGGCCAG AGGTTCCTGG TCACGGGATT CTCAGAACCC 
AGAAGAGCAG TCCGTGCTTC CTCCACAGGG TTTAAGAGAG AAGATGGGGC TGGAGAAAAG 
GAGAAATCTC TCAACCACAA AACACCCGAA CCTGTTCAGC CAGTGCCTGA AGAAGAAAGC 
CATAAAGATA GCGAAGTCCC CAAAGAAAAG CTGGCTGACG ACCAGGGAGA CATGGATCTC 
CAGATCAGCC CAGACCGGAA AACCTCCACT GACTTTTCTG ACGTCATTAA GCAAGAACTG 
GAAGACAATG ACAAATACCA GCAGTTCCGT ATGACTGAGG AGCCTGAGAA GGCACAGCTT 
CACTTAGACC AAGTGCTCAC TAGTCCTTTC AACACGACAT TTCCACTAGA TTACATGAAA 
GATGAGTTCC TCCCAGCCCT GTCTTTGCAG AGTGGCGCTT TACATGGCAG TTCTGAGAGC 
CTGAAGCCCG AAGGGGTGGC AGATTCTCCG TGTGGCAGCC TGATGGAAGG GACCCCTCAG 
ATTAGTTCAG AGGAAAGCTA TAAACATGAG GGCCTAGCCG AGACCCCCGA GACAAGCCCA 
GAGAGCCTTT CCTTCTCGCC AAAGAAAAGT GAGGAGCAGA TTGGGGAAAC ACATGAAACT 
ACCAAGTTAG AAACAGCACC AGAAAGTCAT TCCGAAAAAG GACATCCCTC AACCAAAGAT 
GTTGCTGATG GTTCTGAAGG GCAAGGTGCT GCAGTCACCG AGGCAGTAGA GTCCTCTGCT 
GAGCATCAGC TGACAGAGGC CACCCCAGAC CCTTCCAAAG ACATATGCCC CCCACAGGAA 
GGTGACAGCC TTGGTGGTCA AAGTGTATCA TTAGCAAAAG AAACATCCGA AGGACTGACT 
GAGGAAGTGA CCTGTGACGA AGGTCCACGT GCACACATTA GTCCATCCCA CGAAACACAA 
ACTGATACCG AAGCTCAGGA ATCCACAGCC ACCAAGCCCT CAGGGGAGGC AGAGGCCTCA 
CCGTTGTCCG AGGCTGCTGT AAAGGCAGGT TCAGGGACTG AATCAAAGCC CCAGGCAGCC 
ATTAGAAGTC CGCAAGGGTT AGAACTCGCA CTCCCTAGCC GCGAGAGTGA GGTTTTCAGC 
CCTGGGGCTG ATGAATCCTT CGCTGTGAGC CACAAAGACT CCCTGGAGGC CAGCCCTGTG 
CTGGAAGATA ACTCTTCGCA CAAAACTCCC GATTCTCTGG AACCAAGTCC TCTGAAGGAA 
TCCCCTTGCC GTGACTCTCT TGAAAGCAGC CCCGTTGAAC CAAAGATGAA GGCTGGAATT 
CTCCCAAGTC ACTTTCCTCT TCCCTCAGCT GTTGCCAAGA CCGAGCTCTT CACGGAAGTG 
GCCTCTGTGC GGTCCCGGCT GCTCCGAGAC CCCGACGGCA GTGCCGAGGA TGACAGCCTC 
GAGCAGACGT CACTCATGGA GAGCTCGGGG AAGAGTCCCC TTTCCCCAGA CACCCCCAGC 
TCCGAAGAAG TTAGCTACGA GGTCACGCCC AAAACCACAG ACACACCAAA ACCAGCTGTG 
ATTCCTGAAT GTGCGGAGGA GGACGACTCA GAAAATGGGG AGAAAAAGAG GTTCACACCG 
GAAGAGGAAA TGTTTAAAAT GGTAACCAAA GTCAAAACGT TTGATGAACT TGAACAAGAA 
GCAAAGCAGA AAAGGGACTA CAAAAGAGAA CCCAAGCAGG AAGAATCTTC TTCATCCTCT 
GATGCAGATG TCGACTGTTC AGCAGACACG GATGAACCAA AACACACAGA GAGTGTCGAG 
GATGAAAGTG ACGCTCCTGT GGTAGTAACA ACAGAGGGCA GGGAGGCTTC TTCCTCCTCA 
GAAAGTGAAC CTGAGTTGAC ACAGCTGAAG AAAGGTGCTG ACTCAGGTCT CTTGGCAGAG 
CCAGTTATTC GAGTGCAGCC TCCTTCCCCA CTTCCATCCA GCATAGACTC AAATTCCAGT 
CCTGAAGAAA TACAATTCCA GCCTATCGTT TCCAAACAGT ACACTTTTAA GATGAATGAA 
GATGCTCAGG AAGATTCAGA TAAATCAGAA GAAGAAAAAG ATTATAAATC TCGCTTACCC 
GAAGACAGTC ATGCTCTTCC CACTGATGGA CCAGATATGT CTTATGATGA TCTGAATGGA 
GATACTTATC AATCAAAACT TTGTGATGGC CTTGAGTATG AGATTGGGAG TCCTAGCGAC 
TCAGCTGCTC CTGTCTCTTC AGGTCTCCGC AGTTCCCCTG GTGACGATGT CAGTGAGCAG 
CTAGTTATCC ATAAGGAATC ACTAGCCCTC CAAGACACTG GTGAGAACGA CCTAGAAGAG 
CTTGATGTTC CTGAAGTCGA ATCTTCACAA GCAGATCCCC CCAGTGAAGG CTCTCCATCT 
TTCTCCTCTT TACCTCTTTG TCCAGCATCT GAAGCAAAAG AATTAGATGA AGACATGGTC 
TCTACAGCTT CTGCTACAGA AATGGAGGTC ACAAAACCTG ACCAAGCTTT TGAGAGCTTA 
CCAGAGGACT GTTCCATTCA AGATTCACTC ATTACTACTC AAACTGATAG ATCATCCTTG 
GATGTTCCAG TGTCAGACCC AACAGAGACT GAGGAAATCT ATGATCCTCA AATCATAAGC 
CCTTATGAAA ATGTTCCTTC TCAATCTTTT TTCTCTGGTG AAGAAAGCAA AACCCAAACG 
GATACAAGGC ACACAAGTTT TCATTCTTCT GAAGAGTATT CTGTTACCAT CACATCCTCT 
GTTGAAGATG TAGTAGAGAC AAGCTCCTCT GATAAAACTG TTTCAAGTAA AGAAGCTAAT 
TTTGAGGAGC AGGTCTTGGA AGTAGAATCC AAACAAGAGA GTACCTTGCA GGAAATACAG 
TCACATGGAG CCTCCTCATC TTTGGAGCCA CCTGTACCAA AAACATCAAC AGTTGTCAGT 
GAACAAATAA GCAAAGTCAT CATCACAAAA ACTGATGTGG ATTCTGATTC CTGGAGTGAA 
ATCCGTGAGG ATGACGAAGC CTTTGAGGCT CGAGTGAAAG AGGAAGAACA GAAGATATTT 
GGTTTGATGG TAGACAGACA GTCACAGGGT ACCACCCCTG ACACCACTCC TGCTAGGACC 
CCAACTGAAG AGGGGACCCC AACCAGTGAG CAAAATCCAT TTCTCTTTCA GGAAGGAAAA 
TTGTTTGAGA TGACCCGAAG TGGTGCCATT GATATGACCA AAAGGTCCTA TGCCGATGAA 
AGCTTTCACT TTTTCCAAAT TGGGCAAGAA TCCAGGGAAG AGGCTGTCTC ACAAGACATG 
AAAGAAGGAG GTACAGGTGC TGAGGCCCCA CAGGTGGAGA CATCAGCTGA GTCATTAACA 
CTCTCAGAAT CAAAAGAAAC AGTGGATGAT GAAGCAGAGT TACTCCCAGA TGACCTGAGT 
GAGGAGGTAG AAGAAATACA CACTTCAGAT ACCCCACTTA GCTCCCAAAT AGACATTTCA 
GCCTCCACAG AAACATCCAT GAAAGAGGCT ACTTCTACAG GGCTTGAGGA CCTACCCACC 
AGGCAAATGG GTGATTCTCT TCCTCCATCC AGCGAGAAGC AGACCACTTG CCCTGACTTC 
CCTGAACAAG TTGTACAAGT TCAGTTAGAT TTTTCCACAG TCACTAGGTC TGTATATTCA 
GATCGGGGTG ATGATTCTCC CGATTCCTCC CCAGAGGAAC AGAAATCAGT CATTGAAATC 
CCTACTGCAA CCATGGAGAA TGTGCCTTCT ACTGAGAGCA AATCCAAAAT CCCTGTAAGG 
ACTATCCCCA CTTCAACCTC AATACCTCCA TCGGTGGAAT ATGAGAGTTC CCTTTCTGAA 
GATTTTCTGC CCAGCCTGGA TGAGGAAAGT AAAGAAGATG AAGCAAAACC AAAGTCTAAA 
ATCCCCGTGA AAGCACCTGC CCAAAGAGTG GAGCAGCAGC CTTCACATCT AGACTCACCT 
CTAAAGAAGA CAGGTCGTCC CCAGGGACAG GACTTGACAA GCAGTGCACC AGATGATAGA 
AGCAAATCTG AATCTGATGC CAGTTTGGAC GTGAAGACCA AATGTCCAGT AGAAACTAGA 
AGTTACACTG AGGCAGAAAT AGAAAGCACA GGTGAGGAAG AGGAGCTTGA GTTAGAATCA 
GAGGAAGGGG CCACAAGACC AAAGATATTT GCATCCCGAT TGCCAGTAAA GAGCAAAAGC 
ACCGCATCTT CCTGCAGGGG GCCCACAAGC CCCACAAAAG ACAATAAGGA GCATTTCTTT 
GATCTTTACA GGAACTCCAT AGAATTCTTT GAGGAGATTA GTGATGAAGC TTCCAAGTTA 
GTGGATAGAC TTACACAGTC AGAAAGGGAG CAAGATTTAG TTTCAGATGA TGAAAGTAGT 
AGTGCCCTGG AAGTTTCAGT AATTGAAAAT CTGCCACCTG TTGAGACCGA GAACTCAATT 
CCTGAGGACC TCTTTGACAC AAGGCCCATT TGGGATGAGT CCATTGAGAC TCTGATCGAA 
CGCATCCCTG ATGAAAATGG CCATGACCAT GCTGAAGATC AACAGGGTGA GCAGGAACGG 
ATTGAGGAAA GGCTGGCATA TATTGCTGAT CACCTTGGCT TCAGCTGGAC AGAGTTGGCA 
AGAGAACTGG ACTTCACTGA AGAGCAAATT CATCAAATTC GAATTGAGAA CCCTAACTCC 
CTGCAGGATC AGAGTCATGC GCTGTTGAAG TACTGGCTAG AAAGGGACGG GAGACATGCT 
ACAGATACCA GTCTCGTTGA ATGTCTCACC AAGATCAACC GAATGGATAT CGTGCATCTC 
ATGGAGACCA GCACAGAAGC TCTCCAGGAG CGCATCGGTC ATAGCTATGC AGAGATTGAG 
CAGACCATTA CACTGGATCA TAGTGAAGGG TTCTCAGTAC TCCAAGAGGA GCTTTGCACT 
GCCCAGCACA AGCAGAAAGA GGAGCATGCT GCTTCTAAAG AGGACGAGGC CTCTGACCAC 
CCTCCCATCG TCTCAGAGGA AGACATTTCT GTTGGTTACT CCACTCTTCA GGATGGCATC 
CCCAAAACTG AGGGTGATGG CTCTGCAACA GAGCTCCTTC CCCAGACTCA AAAGGAGCAA 
GTTCAACAGG ATTTCTCAGG GAAAATGCAA GACCTGCCTG AGGAGTCGGC TCTTGAACAG 
CAAGAATACT TCGTGACAAC CCCAGGGACA GAAATGTCAG AGACTCAGAA GGCCACGGGA 
ACATCGGGCT CCCCCACCAA GACACCTGAG GAAATTGTCA CCCCTTCCGA GGAGGAGAGG 
CTGTATCTCC AAACCCCAAC ATCGAGTGAG CGGGGAGGCT CTCCAGTTGT GCAGGAACCT 
GAGGAGCCCC CCGAACGCAG GGAGGAGAGT TCACCTCGGA AAACCAGCCT CGTGATCGTG 
GAATCGGCTG AGGACCAGCC TCAGGCCTTC GAGAGGCTTG ATGAAGAGGC TGCTTTTCAA 
AAGGAGCTAA CAGAGGAATT AGGTGAGCTG GAGGCCAGCT CAGATGAGGA GGCGATGGTA 
ACAACCAGGG TTGTCCGCCG ACGATTGATT ATTCAGGGAG ATGATATGCC TGACATACCC 
CCAGAAACAG TCACAGAAGA AGAATACATC GATGAGCATG GACACACCGT GGTAAAGAAG 
GTTACTAGGA AAATCATTAG GCGGTTCATC TCCTCTGACG GCACAGAGAA AGAGGAGATT 
ACGATGCAGG GAGCACCACA GGATTCTATC AGCGTTGATG AAGGGGATGG TTATTCCAAA 
GTCATAAAGC GCATTGTATT GAAGAGTGAC ACCGAGCAGT CAGAGGTAAC TTTGTCTGAG 
CCCAGCATTC TGTCCAGTAC CTCACAATTT CAGGCTGAAC CAGTGGAAGG CCGTAGAGTC 
AGCAAAGTTG TTAAGACAAC TGTGGTCCAT GGTGAGAGGA TGGAGAAACA TCTGGGGGAT 
TCTAGTTTAG CCACTGATCT TCCTTCAGCC AAAGATGACT TTGAAGAGGC TTTGAGTTAC 
ACAGGTAGCC ACATGAAAGT CTACTCCCCC AGTTTAGTAG AGAATGAAGT CCTGAAGGAG 
GATGGATCAA TAATTAGAAG GACGACAATG AGTAAAGCTA GAACCCAGAA GAGGGCTGTG 
GTGAAGGACC AGCAGGGAAA ACGCATTTAC TTGGAGCACC TGGAGGATGT CCCAGAAGCG 
CTAGACCAGG ACGACCTCCA GCACGACCTC CAGCAACTCC TTCGGCATTT CTGCAAGGAG 
GACTCGAAGC AAGAGGCCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Gene Symbol	ANK2
Entrez Gene ID	539238
Full Name	ankyrin-2
Synonyms	ANK2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

ANK2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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