DMD gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015470523.1	XP_015326009.1	dystrophin
XM_005228315.4	XP_005228372.3	dystrophin isoform X10
XM_024988368.1	XP_024844136.1	dystrophin isoform X12
XM_005228316.4	XP_005228373.1	dystrophin isoform X14
XM_024988367.1	XP_024844135.1	dystrophin isoform X9
XM_005228317.4	XP_005228374.1	dystrophin isoform X11
XM_024988359.1	XP_024844127.1	dystrophin isoform X2
XM_024988362.1	XP_024844130.1	dystrophin isoform X5
XM_024988366.1	XP_024844134.1	dystrophin isoform X8
XM_015461738.1	XP_015317224.1	dystrophin
XM_024988361.1	XP_024844129.1	dystrophin isoform X4
XM_024988358.1	XP_024844126.1	dystrophin isoform X1
XM_024988360.1	XP_024844128.1	dystrophin isoform X3
XM_024988363.1	XP_024844131.1	dystrophin isoform X6
XM_024988364.1	XP_024844132.1	dystrophin isoform X7
XM_024988369.1	XP_024844137.1	dystrophin isoform X13

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa35706	XM_005228316.4 Latest version!	Bos taurus dystrophin (DMD), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa35707	XM_005228317.4 Latest version!	Bos taurus dystrophin (DMD), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa238546	XM_005228315.4 Latest version!	Bos taurus dystrophin (DMD), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa238547	XM_024988368.1 Latest version!	Bos taurus dystrophin (DMD), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa39886	XM_024988369.1 Latest version!	Bos taurus dystrophin (DMD), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OBa238537	XM_024988358.1 Latest version!	Bos taurus dystrophin (DMD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa238538	XM_024988359.1 Latest version!	Bos taurus dystrophin (DMD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa238539	XM_024988360.1 Latest version!	Bos taurus dystrophin (DMD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa238540	XM_024988361.1 Latest version!	Bos taurus dystrophin (DMD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa238541	XM_024988362.1 Latest version!	Bos taurus dystrophin (DMD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa238542	XM_024988363.1 Latest version!	Bos taurus dystrophin (DMD), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa238543	XM_024988364.1 Latest version!	Bos taurus dystrophin (DMD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa238544	XM_024988366.1 Latest version!	Bos taurus dystrophin (DMD), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa238545	XM_024988367.1 Latest version!	Bos taurus dystrophin (DMD), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

DMD ( XM_005228316.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228373.1 Bos taurus dystrophin (DMD), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa35706
Clone ID Related Accession (Same CDS sequence)	XM_005228316.4
Accession Version	XM_005228316.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228316.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAAC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCTAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCGGC TTATAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTTTTGAG CCTGTCAGCC 
GCATGTGATG CCTTGGACCA GCACAACCTC AAGCAAAACG ACCAGCCCAT GGACATCCTG 
CAGATCATTA ACTGTTTGAC CACTATTTAT GACCGCCTGG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGATACG 
GGACGAACAG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCGCATTTGG AAGACAAGTA CAGATACCTT TTCAAGCAAG TGGCCAGTTC AACAGGATTT 
TGTGACCAGC GCAGACTGGG CCTCCTTCTA CATGACTCTA TCCAAATCCC AAGACAGTTG 
GGTGAAGTTG CATCCTTTGG GGGCAGTAAC ATTGAGCCAA GTGTCAGGAG CTGCTTCCAA 
TTCGCTAATA ATAAGCCTGA GATCGAAGCA GCTCTCTTCC TCGATTGGAT GAGACTAGAA 
CCCCAGTCCA TGGTGTGGCT GCCCGTCCTG CACAGGGTGG CTGCCGCGGA GACCGCCAAG 
CATCAGGCCA AGTGTAACAT CTGCAAGGAG TGTCCAATCA TCGGCTTCAG GTACAGAAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGCTGCTTTT TCTCTGGCCG CGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TATTGTACTC CGACTACATC GGGAGAAGAT 
GTTCGAGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGCA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACGGTCTTAG AGGGGGACAA CATGGAAACT 
CCTGTTACTC TGATCAACTT CTGGCCGGTA GACTCTGCGC CTGCCTCGTC CCCTCAGCTT 
TCACACGATG ATACTCATTC ACGCATTGAA CATTATGCTA GCAGGCTAGC AGAAATGGAA 
AACAGCAGTG GATCTTATCT AAATGATAGC ATCTCTCCTA ATGAGAGCAT AGATGATGAA 
CATTTGTTAA TCCAGCATTA CTGCCAAAGT TTGAACCAGG ACTCCCCCCT GAGCCAGCCT 
CGTAGTCCTG CCCAGATCTT GATTTCCTTA GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA 
ATCCTAGCAG ATCTTGAGGA AGAAAACAGG AATCTGCAGG CTGAATATGA TCGTCTCAAG 
GAGCAGCATG AACATAAAGG CCTGTCCCCA CTGCCGTCCC CTCCTGAGAT GATGCCCACC 
TCGCCACAGA GTCCCCGGGA TGCTGAGCTC ATTGCTGAGG CCAAGCTACT GCGTCAACAC 
AAAGGCCGCC TGGAAGCCAG GATGCAAATC CTGGAAGACC ACAATAAACA GCTGGAGTCC 
CAGTTACACA GGCTCAGGCA GCTGCTGGAG CAACCCCAGG CTGAGGCCAA GGTGAATGGC 
ACAACGGTGT CCTCTCCTTC TACCTCTCTT CAGAGGTCAG ACAGCAGCCA GCCTATGCTG 
CTCCGGGTGG TTGGCAGTCA AACTTCAGAA TCCATGGGCG AGGAAGACCT TCTCAGTCCT 
CCCCAGGACT CAAGCACAGG GTTAGAGGAA GTGATGGAGC AACTCAACAA CTCCTTCCCT 
AGTTCCAGAG GACACAATGT AGGAAGCCTT TTCCACTTGG CAGATGATTT GGGCAGAGCG 
ATGGAGTCCT TAGTTTCAGT TATGACAGAT GAAGAAGGGG CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228373.1
CDS	161..2068
Translation	MREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSSGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKEQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDSSTGLEEVMEQLNNSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_005228316.4
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophin (DMD), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228316.4

ORF Insert Sequence:

ATGAGGGAAC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCTAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCGGC TTATAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTTTTGAG CCTGTCAGCC 
GCATGTGATG CCTTGGACCA GCACAACCTC AAGCAAAACG ACCAGCCCAT GGACATCCTG 
CAGATCATTA ACTGTTTGAC CACTATTTAT GACCGCCTGG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGATACG 
GGACGAACAG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCGCATTTGG AAGACAAGTA CAGATACCTT TTCAAGCAAG TGGCCAGTTC AACAGGATTT 
TGTGACCAGC GCAGACTGGG CCTCCTTCTA CATGACTCTA TCCAAATCCC AAGACAGTTG 
GGTGAAGTTG CATCCTTTGG GGGCAGTAAC ATTGAGCCAA GTGTCAGGAG CTGCTTCCAA 
TTCGCTAATA ATAAGCCTGA GATCGAAGCA GCTCTCTTCC TCGATTGGAT GAGACTAGAA 
CCCCAGTCCA TGGTGTGGCT GCCCGTCCTG CACAGGGTGG CTGCCGCGGA GACCGCCAAG 
CATCAGGCCA AGTGTAACAT CTGCAAGGAG TGTCCAATCA TCGGCTTCAG GTACAGAAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGCTGCTTTT TCTCTGGCCG CGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TATTGTACTC CGACTACATC GGGAGAAGAT 
GTTCGAGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGCA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACGGTCTTAG AGGGGGACAA CATGGAAACT 
CCTGTTACTC TGATCAACTT CTGGCCGGTA GACTCTGCGC CTGCCTCGTC CCCTCAGCTT 
TCACACGATG ATACTCATTC ACGCATTGAA CATTATGCTA GCAGGCTAGC AGAAATGGAA 
AACAGCAGTG GATCTTATCT AAATGATAGC ATCTCTCCTA ATGAGAGCAT AGATGATGAA 
CATTTGTTAA TCCAGCATTA CTGCCAAAGT TTGAACCAGG ACTCCCCCCT GAGCCAGCCT 
CGTAGTCCTG CCCAGATCTT GATTTCCTTA GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA 
ATCCTAGCAG ATCTTGAGGA AGAAAACAGG AATCTGCAGG CTGAATATGA TCGTCTCAAG 
GAGCAGCATG AACATAAAGG CCTGTCCCCA CTGCCGTCCC CTCCTGAGAT GATGCCCACC 
TCGCCACAGA GTCCCCGGGA TGCTGAGCTC ATTGCTGAGG CCAAGCTACT GCGTCAACAC 
AAAGGCCGCC TGGAAGCCAG GATGCAAATC CTGGAAGACC ACAATAAACA GCTGGAGTCC 
CAGTTACACA GGCTCAGGCA GCTGCTGGAG CAACCCCAGG CTGAGGCCAA GGTGAATGGC 
ACAACGGTGT CCTCTCCTTC TACCTCTCTT CAGAGGTCAG ACAGCAGCCA GCCTATGCTG 
CTCCGGGTGG TTGGCAGTCA AACTTCAGAA TCCATGGGCG AGGAAGACCT TCTCAGTCCT 
CCCCAGGACT CAAGCACAGG GTTAGAGGAA GTGATGGAGC AACTCAACAA CTCCTTCCCT 
AGTTCCAGAG GACACAATGT AGGAAGCCTT TTCCACTTGG CAGATGATTT GGGCAGAGCG 
ATGGAGTCCT TAGTTTCAGT TATGACAGAT GAAGAAGGGG CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_005228317.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228374.1 Bos taurus dystrophin (DMD), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa35707
Clone ID Related Accession (Same CDS sequence)	XM_005228317.4
Accession Version	XM_005228317.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228317.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAAC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCTAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCGGC TTATAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTTTTGAG CCTGTCAGCC 
GCATGTGATG CCTTGGACCA GCACAACCTC AAGCAAAACG ACCAGCCCAT GGACATCCTG 
CAGATCATTA ACTGTTTGAC CACTATTTAT GACCGCCTGG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGATACG 
GGACGAACAG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCGCATTTGG AAGACAAGTA CAGATACCTT TTCAAGCAAG TGGCCAGTTC AACAGGATTT 
TGTGACCAGC GCAGACTGGG CCTCCTTCTA CATGACTCTA TCCAAATCCC AAGACAGTTG 
GGTGAAGTTG CATCCTTTGG GGGCAGTAAC ATTGAGCCAA GTGTCAGGAG CTGCTTCCAA 
TTCGCTAATA ATAAGCCTGA GATCGAAGCA GCTCTCTTCC TCGATTGGAT GAGACTAGAA 
CCCCAGTCCA TGGTGTGGCT GCCCGTCCTG CACAGGGTGG CTGCCGCGGA GACCGCCAAG 
CATCAGGCCA AGTGTAACAT CTGCAAGGAG TGTCCAATCA TCGGCTTCAG GTACAGAAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGCTGCTTTT TCTCTGGCCG CGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TATTGTACTC CGACTACATC GGGAGAAGAT 
GTTCGAGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGCA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACGGTCTTAG AGGGGGACAA CATGGAAACG 
CCTGCCTCGT CCCCTCAGCT TTCACACGAT GATACTCATT CACGCATTGA ACATTATGCT 
AGCAGGCTAG CAGAAATGGA AAACAGCAGT GGATCTTATC TAAATGATAG CATCTCTCCT 
AATGAGAGCA TAGATGATGA ACATTTGTTA ATCCAGCATT ACTGCCAAAG TTTGAACCAG 
GACTCCCCCC TGAGCCAGCC TCGTAGTCCT GCCCAGATCT TGATTTCCTT AGAGAGTGAG 
GAAAGAGGGG AGCTAGAGAG AATCCTAGCA GATCTTGAGG AAGAAAACAG GAATCTGCAG 
GCTGAATATG ATCGTCTCAA GGAGCAGCAT GAACATAAAG GCCTGTCCCC ACTGCCGTCC 
CCTCCTGAGA TGATGCCCAC CTCGCCACAG AGTCCCCGGG ATGCTGAGCT CATTGCTGAG 
GCCAAGCTAC TGCGTCAACA CAAAGGCCGC CTGGAAGCCA GGATGCAAAT CCTGGAAGAC 
CACAATAAAC AGCTGGAGTC CCAGTTACAC AGGCTCAGGC AGCTGCTGGA GCAACCCCAG 
GCTGAGGCCA AGGTGAATGG CACAACGGTG TCCTCTCCTT CTACCTCTCT TCAGAGGTCA 
GACAGCAGCC AGCCTATGCT GCTCCGGGTG GTTGGCAGTC AAACTTCAGA ATCCATGGGC 
GAGGAAGACC TTCTCAGTCC TCCCCAGGAC TCAAGCACAG GGTTAGAGGA AGTGATGGAG 
CAACTCAACA ACTCCTTCCC TAGTTCCAGA GGACACAATG TAGGAAGCCT TTTCCACTTG 
GCAGATGATT TGGGCAGAGC GATGGAGTCC TTAGTTTCAG TTATGACAGA TGAAGAAGGG 
GCAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228374.1
CDS	137..2005
Translation	MREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPASSPQLSHDDTHSRIEHYASRLAEMENSSGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKEQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDSSTGLEEVMEQLNNSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_005228317.4
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophin (DMD), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228317.4

ORF Insert Sequence:

ATGAGGGAAC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCTAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCGGC TTATAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTTTTGAG CCTGTCAGCC 
GCATGTGATG CCTTGGACCA GCACAACCTC AAGCAAAACG ACCAGCCCAT GGACATCCTG 
CAGATCATTA ACTGTTTGAC CACTATTTAT GACCGCCTGG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGATACG 
GGACGAACAG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCGCATTTGG AAGACAAGTA CAGATACCTT TTCAAGCAAG TGGCCAGTTC AACAGGATTT 
TGTGACCAGC GCAGACTGGG CCTCCTTCTA CATGACTCTA TCCAAATCCC AAGACAGTTG 
GGTGAAGTTG CATCCTTTGG GGGCAGTAAC ATTGAGCCAA GTGTCAGGAG CTGCTTCCAA 
TTCGCTAATA ATAAGCCTGA GATCGAAGCA GCTCTCTTCC TCGATTGGAT GAGACTAGAA 
CCCCAGTCCA TGGTGTGGCT GCCCGTCCTG CACAGGGTGG CTGCCGCGGA GACCGCCAAG 
CATCAGGCCA AGTGTAACAT CTGCAAGGAG TGTCCAATCA TCGGCTTCAG GTACAGAAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGCTGCTTTT TCTCTGGCCG CGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TATTGTACTC CGACTACATC GGGAGAAGAT 
GTTCGAGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGCA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACGGTCTTAG AGGGGGACAA CATGGAAACG 
CCTGCCTCGT CCCCTCAGCT TTCACACGAT GATACTCATT CACGCATTGA ACATTATGCT 
AGCAGGCTAG CAGAAATGGA AAACAGCAGT GGATCTTATC TAAATGATAG CATCTCTCCT 
AATGAGAGCA TAGATGATGA ACATTTGTTA ATCCAGCATT ACTGCCAAAG TTTGAACCAG 
GACTCCCCCC TGAGCCAGCC TCGTAGTCCT GCCCAGATCT TGATTTCCTT AGAGAGTGAG 
GAAAGAGGGG AGCTAGAGAG AATCCTAGCA GATCTTGAGG AAGAAAACAG GAATCTGCAG 
GCTGAATATG ATCGTCTCAA GGAGCAGCAT GAACATAAAG GCCTGTCCCC ACTGCCGTCC 
CCTCCTGAGA TGATGCCCAC CTCGCCACAG AGTCCCCGGG ATGCTGAGCT CATTGCTGAG 
GCCAAGCTAC TGCGTCAACA CAAAGGCCGC CTGGAAGCCA GGATGCAAAT CCTGGAAGAC 
CACAATAAAC AGCTGGAGTC CCAGTTACAC AGGCTCAGGC AGCTGCTGGA GCAACCCCAG 
GCTGAGGCCA AGGTGAATGG CACAACGGTG TCCTCTCCTT CTACCTCTCT TCAGAGGTCA 
GACAGCAGCC AGCCTATGCT GCTCCGGGTG GTTGGCAGTC AAACTTCAGA ATCCATGGGC 
GAGGAAGACC TTCTCAGTCC TCCCCAGGAC TCAAGCACAG GGTTAGAGGA AGTGATGGAG 
CAACTCAACA ACTCCTTCCC TAGTTCCAGA GGACACAATG TAGGAAGCCT TTTCCACTTG 
GCAGATGATT TGGGCAGAGC GATGGAGTCC TTAGTTTCAG TTATGACAGA TGAAGAAGGG 
GCAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_005228315.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228372.3 Bos taurus dystrophin (DMD), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa238546
Clone ID Related Accession (Same CDS sequence)	XM_005228315.4
Accession Version	XM_005228315.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228315.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACACA GGAAGACATA CCATGTAAAG GACCTCCAAG GAGAAATTGA AGCTCACACA 
GATATCTATC ACAACCTAGA TGAAAATGGC CAAAAAATCC TGAGATCCCT GGAAGGTTCT 
GATGACGCGG TCCTATTACA AAGACGTTTG GATAATATGA ATTTCAAGTG GAGTGAGCTG 
CGGAAAAAGT CTCTCAATAT CAGGTCCCAT TTGGAAGCCA GTTCTGACCA GTGGAAGCGT 
CTGCACCTTT CTCTTCAGGA ACTTCTGGTG TGGCTACAGC TGAAAGACGA TGAGTTGAGC 
CGTCAGGCAC CTATTGGAGG CGATTTCCCA GCAGTTCAGA AGCAGAATGA TATACACAGG 
GCCTTCAAGA GGGAACTGAA AACGAAAGAA CCTGTAATCA TGAGTACTCT TGAGACTGTA 
CGAATATTTC TGACAGAGCA GCCTTTGGAA GGACTAGAGA AACTCTACCA GGAGCCCAGA 
GAGCTGCCGC CTGAGGAGAA AGCCCAGAAT GTCACACGAC TCCTACGAAA GCAGGCTGAG 
GAGGTCAACA CAGAGTGGGA AAAATTGAAC CTACACTCGG CTGACTGGCA GAGAAAAATA 
GACGAGGCCC TCGAAAGACT CCAAGAACTG CAGGAGGCCA CGGACGAGCT GGACCTCAAA 
CTGCGCCAAG CTGAGGTGAT CAAGGGATCA TGGCAGCCCG TGGGCGATCT TCTCATTGAC 
TCCCTCCAGG ATCACCTCGA GAAAGTCAAG GCACTTCGAG GAGAAATTGC ACCTCTCAAA 
GAGAATGTAA GCCACGTCAG TGACCTTGCT CGCCAACTCA CCACGTTGGG CATTCAGCTT 
TCACCATATA ATCTCAGCAC TCTGGAAGAC CTGAACACCA GATGGAAGCT TCTGCAGGTG 
GCTGTTGAGG ACCGCATCAG GCAGCTGCAT GAAGCCCACA GGGACTTTGG CCCAGCGTCC 
CAGCACTTCC TTTCCACTTC TGTCCAGGGT CCCTGGGAGA GAGCCATCTC ACCAAACAAA 
GTGCCCTACT ATATCAACCA CGAGACCCAA ACAACTTGCT GGGACCATCC TAAAATGACA 
GAGCTCTACC AGTCTTTAGC TGACCTGAAT AATGTCAGGT TCTCGGCTTA TAGGACTGCC 
ATGAAACTCC GAAGACTGCA GAAGGCCCTT TGCTTGGATC TTTTGAGCCT GTCAGCCGCA 
TGTGATGCCT TGGACCAGCA CAACCTCAAG CAAAACGACC AGCCCATGGA CATCCTGCAG 
ATCATTAACT GTTTGACCAC TATTTATGAC CGCCTGGAGC AAGAGCACAA CAATCTGGTC 
AACGTCCCTC TCTGCGTGGA TATGTGTCTC AATTGGCTGC TGAATGTTTA TGATACGGGA 
CGAACAGGGA GGATCCGGGT CCTGTCTTTT AAAACTGGCA TCATTTCTCT GTGTAAAGCG 
CATTTGGAAG ACAAGTACAG ATACCTTTTC AAGCAAGTGG CCAGTTCAAC AGGATTTTGT 
GACCAGCGCA GACTGGGCCT CCTTCTACAT GACTCTATCC AAATCCCAAG ACAGTTGGGT 
GAAGTTGCAT CCTTTGGGGG CAGTAACATT GAGCCAAGTG TCAGGAGCTG CTTCCAATTC 
GCTAATAATA AGCCTGAGAT CGAAGCAGCT CTCTTCCTCG ATTGGATGAG ACTAGAACCC 
CAGTCCATGG TGTGGCTGCC CGTCCTGCAC AGGGTGGCTG CCGCGGAGAC CGCCAAGCAT 
CAGGCCAAGT GTAACATCTG CAAGGAGTGT CCAATCATCG GCTTCAGGTA CAGAAGTCTA 
AAGCACTTTA ATTATGACAT CTGCCAAAGC TGCTTTTTCT CTGGCCGCGT TGCAAAAGGC 
CATAAAATGC ACTATCCCAT GGTGGAATAT TGTACTCCGA CTACATCGGG AGAAGATGTT 
CGAGACTTTG CCAAGGTACT AAAAAACAAA TTTCGCACCA AAAGGTATTT TGCGAAGCAT 
CCCCGAATGG GCTACCTGCC AGTGCAGACG GTCTTAGAGG GGGACAACAT GGAAACTCCT 
GTTACTCTGA TCAACTTCTG GCCGGTAGAC TCTGCGCCTG CCTCGTCCCC TCAGCTTTCA 
CACGATGATA CTCATTCACG CATTGAACAT TATGCTAGCA GGCTAGCAGA AATGGAAAAC 
AGCAGTGGAT CTTATCTAAA TGATAGCATC TCTCCTAATG AGAGCATAGA TGATGAACAT 
TTGTTAATCC AGCATTACTG CCAAAGTTTG AACCAGGACT CCCCCCTGAG CCAGCCTCGT 
AGTCCTGCCC AGATCTTGAT TTCCTTAGAG AGTGAGGAAA GAGGGGAGCT AGAGAGAATC 
CTAGCAGATC TTGAGGAAGA AAACAGGAAT CTGCAGGCTG AATATGATCG TCTCAAGGAG 
CAGCATGAAC ATAAAGGCCT GTCCCCACTG CCGTCCCCTC CTGAGATGAT GCCCACCTCG 
CCACAGAGTC CCCGGGATGC TGAGCTCATT GCTGAGGCCA AGCTACTGCG TCAACACAAA 
GGCCGCCTGG AAGCCAGGAT GCAAATCCTG GAAGACCACA ATAAACAGCT GGAGTCCCAG 
TTACACAGGC TCAGGCAGCT GCTGGAGCAA CCCCAGGCTG AGGCCAAGGT GAATGGCACA 
ACGGTGTCCT CTCCTTCTAC CTCTCTTCAG AGGTCAGACA GCAGCCAGCC TATGCTGCTC 
CGGGTGGTTG GCAGTCAAAC TTCAGAATCC ATGGGCGAGG AAGACCTTCT CAGTCCTCCC 
CAGGACTCAA GCACAGGGTT AGAGGAAGTG ATGGAGCAAC TCAACAACTC CTTCCCTAGT 
TCCAGAGGAC ACAATGTAGG AAGCCTTTTC CACTTGGCAG ATGATTTGGG CAGAGCGATG 
GAGTCCTTAG TTTCAGTTAT GACAGATGAA GAAGGGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228372.3
CDS	40..2964
Translation	MLHRKTYHVKDLQGEIEAHTDIYHNLDENGQKILRSLEGSDDAVLLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDIHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEEKAQNVTRLLRKQAEEVNTEWEKLNLHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVSHVSDLARQLTTLGIQLSPYNLSTLEDLNTRWKLLQVAVEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSSGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKEQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDSSTGLEEVMEQLNNSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_005228315.4
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophin (DMD), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228315.4

ORF Insert Sequence:

ATGTTACACA GGAAGACATA CCATGTAAAG GACCTCCAAG GAGAAATTGA AGCTCACACA 
GATATCTATC ACAACCTAGA TGAAAATGGC CAAAAAATCC TGAGATCCCT GGAAGGTTCT 
GATGACGCGG TCCTATTACA AAGACGTTTG GATAATATGA ATTTCAAGTG GAGTGAGCTG 
CGGAAAAAGT CTCTCAATAT CAGGTCCCAT TTGGAAGCCA GTTCTGACCA GTGGAAGCGT 
CTGCACCTTT CTCTTCAGGA ACTTCTGGTG TGGCTACAGC TGAAAGACGA TGAGTTGAGC 
CGTCAGGCAC CTATTGGAGG CGATTTCCCA GCAGTTCAGA AGCAGAATGA TATACACAGG 
GCCTTCAAGA GGGAACTGAA AACGAAAGAA CCTGTAATCA TGAGTACTCT TGAGACTGTA 
CGAATATTTC TGACAGAGCA GCCTTTGGAA GGACTAGAGA AACTCTACCA GGAGCCCAGA 
GAGCTGCCGC CTGAGGAGAA AGCCCAGAAT GTCACACGAC TCCTACGAAA GCAGGCTGAG 
GAGGTCAACA CAGAGTGGGA AAAATTGAAC CTACACTCGG CTGACTGGCA GAGAAAAATA 
GACGAGGCCC TCGAAAGACT CCAAGAACTG CAGGAGGCCA CGGACGAGCT GGACCTCAAA 
CTGCGCCAAG CTGAGGTGAT CAAGGGATCA TGGCAGCCCG TGGGCGATCT TCTCATTGAC 
TCCCTCCAGG ATCACCTCGA GAAAGTCAAG GCACTTCGAG GAGAAATTGC ACCTCTCAAA 
GAGAATGTAA GCCACGTCAG TGACCTTGCT CGCCAACTCA CCACGTTGGG CATTCAGCTT 
TCACCATATA ATCTCAGCAC TCTGGAAGAC CTGAACACCA GATGGAAGCT TCTGCAGGTG 
GCTGTTGAGG ACCGCATCAG GCAGCTGCAT GAAGCCCACA GGGACTTTGG CCCAGCGTCC 
CAGCACTTCC TTTCCACTTC TGTCCAGGGT CCCTGGGAGA GAGCCATCTC ACCAAACAAA 
GTGCCCTACT ATATCAACCA CGAGACCCAA ACAACTTGCT GGGACCATCC TAAAATGACA 
GAGCTCTACC AGTCTTTAGC TGACCTGAAT AATGTCAGGT TCTCGGCTTA TAGGACTGCC 
ATGAAACTCC GAAGACTGCA GAAGGCCCTT TGCTTGGATC TTTTGAGCCT GTCAGCCGCA 
TGTGATGCCT TGGACCAGCA CAACCTCAAG CAAAACGACC AGCCCATGGA CATCCTGCAG 
ATCATTAACT GTTTGACCAC TATTTATGAC CGCCTGGAGC AAGAGCACAA CAATCTGGTC 
AACGTCCCTC TCTGCGTGGA TATGTGTCTC AATTGGCTGC TGAATGTTTA TGATACGGGA 
CGAACAGGGA GGATCCGGGT CCTGTCTTTT AAAACTGGCA TCATTTCTCT GTGTAAAGCG 
CATTTGGAAG ACAAGTACAG ATACCTTTTC AAGCAAGTGG CCAGTTCAAC AGGATTTTGT 
GACCAGCGCA GACTGGGCCT CCTTCTACAT GACTCTATCC AAATCCCAAG ACAGTTGGGT 
GAAGTTGCAT CCTTTGGGGG CAGTAACATT GAGCCAAGTG TCAGGAGCTG CTTCCAATTC 
GCTAATAATA AGCCTGAGAT CGAAGCAGCT CTCTTCCTCG ATTGGATGAG ACTAGAACCC 
CAGTCCATGG TGTGGCTGCC CGTCCTGCAC AGGGTGGCTG CCGCGGAGAC CGCCAAGCAT 
CAGGCCAAGT GTAACATCTG CAAGGAGTGT CCAATCATCG GCTTCAGGTA CAGAAGTCTA 
AAGCACTTTA ATTATGACAT CTGCCAAAGC TGCTTTTTCT CTGGCCGCGT TGCAAAAGGC 
CATAAAATGC ACTATCCCAT GGTGGAATAT TGTACTCCGA CTACATCGGG AGAAGATGTT 
CGAGACTTTG CCAAGGTACT AAAAAACAAA TTTCGCACCA AAAGGTATTT TGCGAAGCAT 
CCCCGAATGG GCTACCTGCC AGTGCAGACG GTCTTAGAGG GGGACAACAT GGAAACTCCT 
GTTACTCTGA TCAACTTCTG GCCGGTAGAC TCTGCGCCTG CCTCGTCCCC TCAGCTTTCA 
CACGATGATA CTCATTCACG CATTGAACAT TATGCTAGCA GGCTAGCAGA AATGGAAAAC 
AGCAGTGGAT CTTATCTAAA TGATAGCATC TCTCCTAATG AGAGCATAGA TGATGAACAT 
TTGTTAATCC AGCATTACTG CCAAAGTTTG AACCAGGACT CCCCCCTGAG CCAGCCTCGT 
AGTCCTGCCC AGATCTTGAT TTCCTTAGAG AGTGAGGAAA GAGGGGAGCT AGAGAGAATC 
CTAGCAGATC TTGAGGAAGA AAACAGGAAT CTGCAGGCTG AATATGATCG TCTCAAGGAG 
CAGCATGAAC ATAAAGGCCT GTCCCCACTG CCGTCCCCTC CTGAGATGAT GCCCACCTCG 
CCACAGAGTC CCCGGGATGC TGAGCTCATT GCTGAGGCCA AGCTACTGCG TCAACACAAA 
GGCCGCCTGG AAGCCAGGAT GCAAATCCTG GAAGACCACA ATAAACAGCT GGAGTCCCAG 
TTACACAGGC TCAGGCAGCT GCTGGAGCAA CCCCAGGCTG AGGCCAAGGT GAATGGCACA 
ACGGTGTCCT CTCCTTCTAC CTCTCTTCAG AGGTCAGACA GCAGCCAGCC TATGCTGCTC 
CGGGTGGTTG GCAGTCAAAC TTCAGAATCC ATGGGCGAGG AAGACCTTCT CAGTCCTCCC 
CAGGACTCAA GCACAGGGTT AGAGGAAGTG ATGGAGCAAC TCAACAACTC CTTCCCTAGT 
TCCAGAGGAC ACAATGTAGG AAGCCTTTTC CACTTGGCAG ATGATTTGGG CAGAGCGATG 
GAGTCCTTAG TTTCAGTTAT GACAGATGAA GAAGGGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_024988368.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844136.1 Bos taurus dystrophin (DMD), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa238547
Clone ID Related Accession (Same CDS sequence)	XM_024988368.1
Accession Version	XM_024988368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAAC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCTAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCGGC TTATAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTTTTGAG CCTGTCAGCC 
GCATGTGATG CCTTGGACCA GCACAACCTC AAGCAAAACG ACCAGCCCAT GGACATCCTG 
CAGATCATTA ACTGTTTGAC CACTATTTAT GACCGCCTGG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGATACG 
GGACGAACAG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCGCATTTGG AAGACAAGTA CAGATACCTT TTCAAGCAAG TGGCCAGTTC AACAGGATTT 
TGTGACCAGC GCAGACTGGG CCTCCTTCTA CATGACTCTA TCCAAATCCC AAGACAGTTG 
GGTGAAGTTG CATCCTTTGG GGGCAGTAAC ATTGAGCCAA GTGTCAGGAG CTGCTTCCAA 
TTCGCTAATA ATAAGCCTGA GATCGAAGCA GCTCTCTTCC TCGATTGGAT GAGACTAGAA 
CCCCAGTCCA TGGTGTGGCT GCCCGTCCTG CACAGGGTGG CTGCCGCGGA GACCGCCAAG 
CATCAGGCCA AGTGTAACAT CTGCAAGGAG TGTCCAATCA TCGGCTTCAG GTACAGAAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGCTGCTTTT TCTCTGGCCG CGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TATTGTACTC CGACTACATC GGGAGAAGAT 
GTTCGAGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGCA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACGGTCTTAG AGGGGGACAA CATGGAAACT 
CCTGTTACTC TGATCAACTT CTGGCCGGTA GACTCTGCGC CTGCCTCGTC CCCTCAGCTT 
TCACACGATG ATACTCATTC ACGCATTGAA CATTATGCTA GCAGGCTAGC AGAAATGGAA 
AACAGCAGTG GATCTTATCT AAATGATAGC ATCTCTCCTA ATGAGAGCAT AGATGATGAA 
CATTTGTTAA TCCAGCATTA CTGCCAAAGT TTGAACCAGG ACTCCCCCCT GAGCCAGCCT 
CGTAGTCCTG CCCAGATCTT GATTTCCTTA GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA 
ATCCTAGCAG ATCTTGAGGA AGAAAACAGG AATCTGCAGG CTGAATATGA TCGTCTCAAG 
GAGCAGCATG AACATAAAGG CCTGTCCCCA CTGCCGTCCC CTCCTGAGAT GATGCCCACC 
TCGCCACAGA GTCCCCGGGA TGCTGAGCTC ATTGCTGAGG CCAAGCTACT GCGTCAACAC 
AAAGGCCGCC TGGAAGCCAG GATGCAAATC CTGGAAGACC ACAATAAACA GCTGGAGTCC 
CAGTTACACA GGCTCAGGCA GCTGCTGGAG CAACCCCAGG CTGAGGCCAA GGTGAATGGC 
ACAACGGTGT CCTCTCCTTC TACCTCTCTT CAGAGGTCAG ACAGCAGCCA GCCTATGCTG 
CTCCGGGTGG TTGGCAGTCA AACTTCAGAA TCCATGGGCG AGGAAGACCT TCTCAGTCCT 
CCCCAGGACT CAAGCACAGG GTTAGAGGAA GTGATGGAGC AACTCAACAA CTCCTTCCCT 
AGTTCCAGAG GAAGAAATAC CCCTGGGAAG CCAATGAGAG AGGACACAAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844136.1
CDS	121..1974
Translation	MREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSSGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKEQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDSSTGLEEVMEQLNNSFPSSRGRNTPGKPMREDTM

Target ORF information:

RefSeq Version	XM_024988368.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophin (DMD), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988368.1

ORF Insert Sequence:

ATGAGGGAAC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCTAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCGGC TTATAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTTTTGAG CCTGTCAGCC 
GCATGTGATG CCTTGGACCA GCACAACCTC AAGCAAAACG ACCAGCCCAT GGACATCCTG 
CAGATCATTA ACTGTTTGAC CACTATTTAT GACCGCCTGG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGATACG 
GGACGAACAG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCGCATTTGG AAGACAAGTA CAGATACCTT TTCAAGCAAG TGGCCAGTTC AACAGGATTT 
TGTGACCAGC GCAGACTGGG CCTCCTTCTA CATGACTCTA TCCAAATCCC AAGACAGTTG 
GGTGAAGTTG CATCCTTTGG GGGCAGTAAC ATTGAGCCAA GTGTCAGGAG CTGCTTCCAA 
TTCGCTAATA ATAAGCCTGA GATCGAAGCA GCTCTCTTCC TCGATTGGAT GAGACTAGAA 
CCCCAGTCCA TGGTGTGGCT GCCCGTCCTG CACAGGGTGG CTGCCGCGGA GACCGCCAAG 
CATCAGGCCA AGTGTAACAT CTGCAAGGAG TGTCCAATCA TCGGCTTCAG GTACAGAAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGCTGCTTTT TCTCTGGCCG CGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TATTGTACTC CGACTACATC GGGAGAAGAT 
GTTCGAGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGCA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACGGTCTTAG AGGGGGACAA CATGGAAACT 
CCTGTTACTC TGATCAACTT CTGGCCGGTA GACTCTGCGC CTGCCTCGTC CCCTCAGCTT 
TCACACGATG ATACTCATTC ACGCATTGAA CATTATGCTA GCAGGCTAGC AGAAATGGAA 
AACAGCAGTG GATCTTATCT AAATGATAGC ATCTCTCCTA ATGAGAGCAT AGATGATGAA 
CATTTGTTAA TCCAGCATTA CTGCCAAAGT TTGAACCAGG ACTCCCCCCT GAGCCAGCCT 
CGTAGTCCTG CCCAGATCTT GATTTCCTTA GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA 
ATCCTAGCAG ATCTTGAGGA AGAAAACAGG AATCTGCAGG CTGAATATGA TCGTCTCAAG 
GAGCAGCATG AACATAAAGG CCTGTCCCCA CTGCCGTCCC CTCCTGAGAT GATGCCCACC 
TCGCCACAGA GTCCCCGGGA TGCTGAGCTC ATTGCTGAGG CCAAGCTACT GCGTCAACAC 
AAAGGCCGCC TGGAAGCCAG GATGCAAATC CTGGAAGACC ACAATAAACA GCTGGAGTCC 
CAGTTACACA GGCTCAGGCA GCTGCTGGAG CAACCCCAGG CTGAGGCCAA GGTGAATGGC 
ACAACGGTGT CCTCTCCTTC TACCTCTCTT CAGAGGTCAG ACAGCAGCCA GCCTATGCTG 
CTCCGGGTGG TTGGCAGTCA AACTTCAGAA TCCATGGGCG AGGAAGACCT TCTCAGTCCT 
CCCCAGGACT CAAGCACAGG GTTAGAGGAA GTGATGGAGC AACTCAACAA CTCCTTCCCT 
AGTTCCAGAG GAAGAAATAC CCCTGGGAAG CCAATGAGAG AGGACACAAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_024988369.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844137.1 Bos taurus dystrophin (DMD), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa39886
Clone ID Related Accession (Same CDS sequence)	XM_024988369.1
Accession Version	XM_024988369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAAC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCTAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCGGC TTATAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTTTTGAG CCTGTCAGCC 
GCATGTGATG CCTTGGACCA GCACAACCTC AAGCAAAACG ACCAGCCCAT GGACATCCTG 
CAGATCATTA ACTGTTTGAC CACTATTTAT GACCGCCTGG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGATACG 
GGACGAACAG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCGCATTTGG AAGACAAGTA CAGATACCTT TTCAAGCAAG TGGCCAGTTC AACAGGATTT 
TGTGACCAGC GCAGACTGGG CCTCCTTCTA CATGACTCTA TCCAAATCCC AAGACAGTTG 
GGTGAAGTTG CATCCTTTGG GGGCAGTAAC ATTGAGCCAA GTGTCAGGAG CTGCTTCCAA 
TTCGCTAATA ATAAGCCTGA GATCGAAGCA GCTCTCTTCC TCGATTGGAT GAGACTAGAA 
CCCCAGTCCA TGGTGTGGCT GCCCGTCCTG CACAGGGTGG CTGCCGCGGA GACCGCCAAG 
CATCAGGCCA AGTGTAACAT CTGCAAGGAG TGTCCAATCA TCGGCTTCAG GTACAGAAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGCTGCTTTT TCTCTGGCCG CGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TATTGTACTC CGACTACATC GGGAGAAGAT 
GTTCGAGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGCA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACGGTCTTAG AGGGGGACAA CATGGAAACG 
CCTGCCTCGT CCCCTCAGCT TTCACACGAT GATACTCATT CACGCATTGA ACATTATGCT 
AGCAGGCTAG CAGAAATGGA AAACAGCAGT GGATCTTATC TAAATGATAG CATCTCTCCT 
AATGAGAGCA TAGATGATGA ACATTTGTTA ATCCAGCATT ACTGCCAAAG TTTGAACCAG 
GACTCCCCCC TGAGCCAGCC TCGTAGTCCT GCCCAGATCT TGATTTCCTT AGAGAGTGAG 
GAAAGAGGGG AGCTAGAGAG AATCCTAGCA GATCTTGAGG AAGAAAACAG GAATCTGCAG 
GCTGAATATG ATCGTCTCAA GGAGCAGCAT GAACATAAAG GCCTGTCCCC ACTGCCGTCC 
CCTCCTGAGA TGATGCCCAC CTCGCCACAG AGTCCCCGGG ATGCTGAGCT CATTGCTGAG 
GCCAAGCTAC TGCGTCAACA CAAAGGCCGC CTGGAAGCCA GGATGCAAAT CCTGGAAGAC 
CACAATAAAC AGCTGGAGTC CCAGTTACAC AGGCTCAGGC AGCTGCTGGA GCAACCCCAG 
GCTGAGGCCA AGGTGAATGG CACAACGGTG TCCTCTCCTT CTACCTCTCT TCAGAGGTCA 
GACAGCAGCC AGCCTATGCT GCTCCGGGTG GTTGGCAGTC AAACTTCAGA ATCCATGGGC 
GAGGAAGACC TTCTCAGTCC TCCCCAGGAC TCAAGCACAG GGTTAGAGGA AGTGATGGAG 
CAACTCAACA ACTCCTTCCC TAGTTCCAGA GGAAGAAATA CCCCTGGGAA GCCAATGAGA 
GAGGACACAA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844137.1
CDS	137..1951
Translation	MREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPASSPQLSHDDTHSRIEHYASRLAEMENSSGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKEQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDSSTGLEEVMEQLNNSFPSSRGRNTPGKPMREDTM

Target ORF information:

RefSeq Version	XM_024988369.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophin (DMD), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988369.1

ORF Insert Sequence:

ATGAGGGAAC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCTAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCGGC TTATAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTTTTGAG CCTGTCAGCC 
GCATGTGATG CCTTGGACCA GCACAACCTC AAGCAAAACG ACCAGCCCAT GGACATCCTG 
CAGATCATTA ACTGTTTGAC CACTATTTAT GACCGCCTGG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGATACG 
GGACGAACAG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCGCATTTGG AAGACAAGTA CAGATACCTT TTCAAGCAAG TGGCCAGTTC AACAGGATTT 
TGTGACCAGC GCAGACTGGG CCTCCTTCTA CATGACTCTA TCCAAATCCC AAGACAGTTG 
GGTGAAGTTG CATCCTTTGG GGGCAGTAAC ATTGAGCCAA GTGTCAGGAG CTGCTTCCAA 
TTCGCTAATA ATAAGCCTGA GATCGAAGCA GCTCTCTTCC TCGATTGGAT GAGACTAGAA 
CCCCAGTCCA TGGTGTGGCT GCCCGTCCTG CACAGGGTGG CTGCCGCGGA GACCGCCAAG 
CATCAGGCCA AGTGTAACAT CTGCAAGGAG TGTCCAATCA TCGGCTTCAG GTACAGAAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGCTGCTTTT TCTCTGGCCG CGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TATTGTACTC CGACTACATC GGGAGAAGAT 
GTTCGAGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGCA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACGGTCTTAG AGGGGGACAA CATGGAAACG 
CCTGCCTCGT CCCCTCAGCT TTCACACGAT GATACTCATT CACGCATTGA ACATTATGCT 
AGCAGGCTAG CAGAAATGGA AAACAGCAGT GGATCTTATC TAAATGATAG CATCTCTCCT 
AATGAGAGCA TAGATGATGA ACATTTGTTA ATCCAGCATT ACTGCCAAAG TTTGAACCAG 
GACTCCCCCC TGAGCCAGCC TCGTAGTCCT GCCCAGATCT TGATTTCCTT AGAGAGTGAG 
GAAAGAGGGG AGCTAGAGAG AATCCTAGCA GATCTTGAGG AAGAAAACAG GAATCTGCAG 
GCTGAATATG ATCGTCTCAA GGAGCAGCAT GAACATAAAG GCCTGTCCCC ACTGCCGTCC 
CCTCCTGAGA TGATGCCCAC CTCGCCACAG AGTCCCCGGG ATGCTGAGCT CATTGCTGAG 
GCCAAGCTAC TGCGTCAACA CAAAGGCCGC CTGGAAGCCA GGATGCAAAT CCTGGAAGAC 
CACAATAAAC AGCTGGAGTC CCAGTTACAC AGGCTCAGGC AGCTGCTGGA GCAACCCCAG 
GCTGAGGCCA AGGTGAATGG CACAACGGTG TCCTCTCCTT CTACCTCTCT TCAGAGGTCA 
GACAGCAGCC AGCCTATGCT GCTCCGGGTG GTTGGCAGTC AAACTTCAGA ATCCATGGGC 
GAGGAAGACC TTCTCAGTCC TCCCCAGGAC TCAAGCACAG GGTTAGAGGA AGTGATGGAG 
CAACTCAACA ACTCCTTCCC TAGTTCCAGA GGAAGAAATA CCCCTGGGAA GCCAATGAGA 
GAGGACACAA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_024988358.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844126.1 Bos taurus dystrophin (DMD), transcript variant X1, mRNA.

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CloneID	OBa238537
Clone ID Related Accession (Same CDS sequence)	XM_024988358.1
Accession Version	XM_024988358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAATTTTCT AAGTTCGGGA AGCAGCACAT AGAAAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTC TTGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCAGGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAATA TCGGAAGCTC TGACATAGTA 
GATGGAAATC ATAAACTGAC TCTTGGCTTG ATTTGGAATA TAATCCTCCA TTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA GAAGATACTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT CAACTTCACC 
ACCAGCTGGT CTGACGGTTT GGCTTTGAAT GCGCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TCGCCAGCAG TCAGCTACAC AACGACTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTACTGG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TACGTCACAT CTCTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCCTTGAAGC CATCCAGGAG GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACAAGAGAAG AACATTTTCA ACTACATCAT CAAATGCACT ACTCTCAACA GATCACTGTC 
AGTCTAGCAC AGGGCTATGA ACGAACTCCT TCCTCTCCTC AGCCTCGCTT CAAGAGCTAT 
GCCTACACGC AGGCTGCTTA TGTCTCCACC TCTGACCCCA CACGGAGCCC ATTTCCTTCA 
CAGCGTTTGG AAGCTCCTGA AGACAAGTCA TTTGGCAGTC CATTGATGGA GACTGAAGTA 
AACCTGGACA GTTACCAGAC AGCTTTAGAA GAAGTACTCT CATGGCTCCT TTCAGCCGAA 
GACACATTGC AAGCACAAGG AGAGATTTCT AATGATGTAG AAGAAGTGAA AGAACAGTTT 
CATACCCACG AGGGATACAT GATGGATTTG ACATCCCATC AGGGACGGGT TGGCAATGTT 
CTCCAATTGG GGAGTCAGCT GATTGGGTCA GGGAAATTAT CAGAAGATGA AGAAACTGAA 
GTGCAAGAAC AAATGAATCT TCTAAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTC ATGGATCTCC AGAATCAGCA GCTGAAAGAG 
TTAAATGCCT GGTTAACAAA AACAGAAGAG AGAACAAGGA AAATGGAAAA AGAGCCCCTT 
GGACCTGATC TTGAGGACCT CAAACGCCAA ATACAGCAAC ATAAGGTGCT TCAAGAAGAC 
CTAGAACAGG AACAAGTCAG AGTCAATTCT CTCACGCACA TGGTGGTGGT TGTAGATGAG 
TCTAGTGGTG ATCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGG 
TGGGCAAACA TCTGTAGGTG GACTGAAGAT CGCTGGGTTC TTTTACAGGA TGTTCTTCTA 
AAATGGCAAC GTTTTACTGA AGAGCAGTGC CTTTTTAGTA CATGGCTTTC AGATAAAGAA 
GATGCATTGA ACAAGATTCC CACAAGTGGC TTTAAGGATC AAAGTGAAAT GTTATCAAGT 
CTTCAAAAAC TGGCCGTTTT AAAAACTGAT CTAGAAAAGA AAAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCATGA TCTCCTTTCA ACACTGAAAA ATACATTGGT TGCCCAAAAG 
ATGGAAGCAT GGTTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA AAAACTTGAA 
AAGAGTTCAA CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACGGTAAC TATGGTGACC ACGAGGGAGC AAATCCTGGT AAAGCATGCC 
CAAGAGGAAC TCCCTCCACC ACCTCCACAA AAGAAGAGGC AGATTATTGT GGATTCTGAA 
ATTAAGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCCGTGTTAC AGAGTCCTGA ATTTGCAGTC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGTATCAAAC AAGCCTCAGA ACAACTGAAC AGCCGGTGGA TCGAGTTCTG CCAATTGCTA 
AGTGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCA GAAAACTGGT TGAAAACTCA ACCCACTACC 
CCATCAGAGC CAACAGCAGT TAAAAGCCAG TTAAAAAATT GTAAGGATGA AGTCAACCGG 
CTGTCAGCTC TTCAGCCTCA AATCGAGAGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCC GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATATGCA GGCCAGAGAG AAAGAGTTAC AGACAATTTT GGACACTTTA 
CCCACCGTGC GGTATCAGGA GACCATGAGT ACCATCCTCA CGTGGATCCA ACAGTCAGAA 
ACCAAACTCT CGATACCTCA GGTTACTGTC ACTGAATACG AGATCATGGA GCAGAGACTT 
GAGGAGTTAC AGGCTTTACA AAGCTCTCTG CAAGAGCACC AAAATGACCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAGGAAA GCACCCTCTC ATATAAGCCA GAGATATCAG 
TCAGAATTTG AAAACATTGA GGGTCGGTGG AAGAAACTCT CTGCCCAGCT GAATGAACGT 
TGCCAAAAGC TAGAGGAGCA AATGGCCAAA CTCCGAAAAC TTCAGAATCA CATAAAAACA 
CTGAAGAAGT GGATGGCTGA AGTTGATGTT TTCTTGAAAG ATGAATGGCC TGCCCTTGGA 
GATTCAGAAA TTCTTAAAAA ACAGCTGAAG CAGTGCAGGC TTTTAGTCAG TGATATTCAG 
ACAATCCAGC CCAGTCTAAA CAGTGTCAAT GAAGGTGGAC AGAAAATAAA AACTGAAGCG 
GAGCCAGAGT TTGCTTCTAG ACTGGAGACA GAGCTCAGAG AACTTAACAC TCAATGGGAT 
TACATATGTC GACAGGTCTA TGCCAGAAAG GAGGCCTTAA AGGGAGGCTT GGATAAAACT 
GTAAGCCTTC AGAAAGATTT GTCAGAGATG CATGAATGGA TGACACAAGC CGAAGAAGAA 
TACCTAGAGA GAGATTTTGA ATATAAAACC CCAGATGAAT TACAGACAGC AGTTGAAGAG 
ATGAAGAGAG CTAAAGAAGA GGCCCAGCAA AAAGAAGCAA AAGTGCAGCT CCTCACTGAG 
TCCGTGAACA GTGTCATAGC TCAAGCTCCA CCTGCAGCAC AAGAGGCCTT AAGAAAGGAA 
CTGGACACTC TGACCACCAA CTACCAGTGG CTCTGCACCA GGCTGAATGG AAAATGCAAG 
ACGTTAGAAG AGGTTTGGGC ATGTTGGCAT GAGTTATTGT CATACTTGGA AAAGGCAAAT 
AAGTGGCTAA ATGAAGTAGA ATTGAAACTT AAGACTACTG AAAATATTCC TGGTGGAGCT 
GAGGAAATCT CTGAGGTGCT CAGTTCACTT GAAAATTTGA TGCAACATTC AGAAGATAAC 
CCGAATCAGA TTCGCATATT GGCACAGACC CTAACAGATG GTGGAGTCAT GGATGAACTG 
ATCAATGAAG AACTCGAGAC ATTTAATTCT CGTTGGAGAG AACTACATGA AGAGGCTGTG 
AGGAGGCAAA AGTTGCTTGA ACAGAGTATC CAGTCTGCCC AGGAGATTGA AAAATCCTTA 
CAGTTAATTC AGGAATCCCT CTCATCAATT GACAAGCAGT TGGCAGCTTA TATCGCTGAC 
AAAGTGGACG CCGCTCAGAT GCCTCAGGAA GCCCAGAAAA TCCAATCTGA TTTGACAAGT 
CATGAGATCA GTTTAGAAGA AATGAAGAAA CATTACCAAG GAAAGGAAAC TGCTCAACGG 
GTACTATCCC AAATTGAAGT GGCACAGAAA AAAATGCAAG ATGTTTCCAT GAAATTTCGA 
TTGTTCCAGA AGCCAGCCAA TTTTGAGCAG CGTCTACAGG AAAGTAAGAT GATTTTAGAT 
GAAGTGAAGA TGCACTTGCC CGCACTGGAA ACAAAGAGTG TTGAACAGGA AGTAGTACAG 
TCACAGTTAA ATCATTGTGT GAACTTGTAT AAAAGTCTGA GTGAAGTGAA GTCTGAAGTG 
GAAATGGTGA TTAAAACTGG ACGTCAAATT GTACAGAAGA AGCAGACGGA AAACCCTAAA 
GAGCTTGATG AAAGAGTAAC GGCTTTGAAA TTGCATTATA ATGAGCTGGG AGCAAAGGTA 
ACAGAAAGAA AGCAACAGTT GGAGAAATGC TTGAAATTGT CCCGTAAGAT GCGAAAGGAA 
ATGAATGCCT TGACAGAATG GCTGGCAGCT ACAGATTTGG AACTGACAAA GAGATCAGCA 
GTTGAAGGAA TGCCTAGTAA TTTGGATTCT GAAGTTGCCT GGGGAAAGGC TACTCAGAAA 
GAGACTGAGA AACAGAAGGT TCACCTAAAG AGCATTACAG AGCTAGGAGA GGCCTTGAAA 
ACGGTTTTGG GCAAGAAGGA GACCTTGGTC GAAGATAAAC TGAGTCTTTT GAATAGTAAC 
TGGATAGCCG TCACTTCTCG AGCAGAAGAG TGGCTAAACC TTTTGTTGGA ATACCAGAAA 
CACATGGAAA CCTTTGACCA GAATGTGGAT CATATCACAA AGTGGATCAT TCAAGCTGAT 
GCACTTCTGG ATGAATCTGA GAAAAAGAAA CCACAGCAAA AAGAAGACAT GCTAAAGCGC 
TTAAAGGCTG AACTGAATGA CATACGCCCA AAGGTGGACT CCACGCGTGA CCAAGCAGCA 
AACTTGATGG CAAACCGCGG CGACCACTGC AGGAAAGTAA TAGAGCCCAA AATCTCAGAG 
CTCAACCATC GATTTGCAGC CATTTCTCAC AGAATTAAGA CTGGAAAGGC CTCCATTCCT 
TTGAAGGAAT TGGAGCAGTT TAACTCAGAT ATACAAAAAT TGCTTGAACC ACTGGAGGCT 
GAAATTCAGC AGGGGGTGAA TCTGAAAGAG GAAGACTTCA ATAAAGATAT GAGTGAAGAC 
AATGAGGGTA CTGTAAAAGA ATTGTTGCAA AGAGGAGACA ACTTACAACA AAGAATCACA 
GATGAGAGAA AGCGAGAGGA AATAAAGATA AAACAGCAGC TGTTACAGAC AAAACATAAT 
GCTCTCAAGG ATTTGAGGTC TCAAAGAAGA AAAAAGGCTC TAGAAATTTC TCACCAGTGG 
TATCAGTACA AGAGGCAGGC TGATGATCTC CTGAAATGCT TGGATGACAT TGAAAAAAAA 
TTAGCCAGCC TACCTGAACC CAGAGATGAA AGGAAAATAA AGGAAATTGA TCGTGAGTTG 
CAGAAGAAGA AAGAGGAGCT GAATGCAGTG CATAGGCAAG CTGAGGGCTT GTCTGAGGAT 
GGGGCAGCAA TGGCAGTGGA GTCAACTCAG ATCCAGCTCA GCAAGCGCTG GCGGGAAATT 
GAGAGCAAAT TTGCTCAGTT TCGAAGACTC AACTTTGCAC AAATTCACAC TGTCCATGAA 
GAATCAGTGA TGGTGATGAC CGAAGACATG CCTTTGGAAA TTTCTTATGT GCCTTCTACT 
TACCTGACTG AGATCACTCA CGTCTTACAA GCCCTTTCAG AAGTGGAACA ACTTCTCAAT 
GCTCCCGACC TCTGTGCTAA AGATTTTCAA GATCTCTTCA AACAAGAGGA ATCTCTGAAG 
AATATAAAAG ACAACCTGCA ACAAATCTCA GGTCGGATTG ATGTTATTCA CAATAAGAAG 
GCGGCAGCAT TGCAAAGCAC CACACCCCCA GAAAAGGCAA GGTTACAGGA AGCTCTCTCC 
AGGCTCGACT TCCAATGGGA AAGAGTGAAC AAAATGTACA AGGACCGACA AGGGCAATTT 
GACAGATCTG TTGAAAAATG GCGACGGTTT CATTATGATA TGAAGATATT TAATCAATGG 
CTAACAGAAG CTGAACACTT TCTCAAAAAG ACACAAATTC CTGAGAACTG GGAACATGCC 
AAATATAAAT GGTATCTTAA GGAACTCCAG GATGGCATCG GGCAGCGGCA AACTGTTGTC 
AGAGTATTGA ATGCAACTGG GGAAGAAATA ATTCAGCAGT CCTCAAAAAT AGATGCCAGT 
ATTCTACAAG AAAAATTGGG AAGCCTGAAT CTGCGGTGGC AGGAGGTCTG CAAACAGCTG 
GCCGAGAGAA AAAAGAGGTT AGAAGAACAA AAGAATATCC TGTCAGAATT TCAAAGAGAT 
TTGAATGAAT TTGTTTTATG GTTGGAAGAA GCAGGTAACA TTTCTAGTAT TCCTCTTGAA 
CCTGGAAATG AGCAGCAACT AAAAGAAAAG CTTGAGGAAG TCAAGTTACT GGCCGAAGAG 
TTGCCCCTGC GTCAGGGAAT TCTAAAACAA TTAAATGAAA CAGGAGGAAC AGTGCTTGTA 
AGTGCTCCCA TAAGCCCAGA AGAGCAAGAT AAACTTGAAA ATAAGCTCAA GCAGACAAAT 
CTTCAGTGGA TAAAGGTTTC TAGGAGTTTA CCTGAGAAAC AGGGAGAAAT TGAGGCTCAC 
ATAAAAGACC TTGGACAGCT TGAAGAGCAA TTAAATCATC TGCTTCTGTG GCTGTCTCCT 
ATTAGGAGTC AGTTGGAAAT TTACAACCAA CCAAATCAGA CAGGACCATT TGACATTAAG 
GAAACTGAAG TAGCAGTTCA AGCTAAACAG CCGGATGTGG AAGGGATTTT GTCTAAAGGG 
CAGAATTTGT ACAAGGAAAA ACCAGCCACT GAGCCAGTGA AGAGGAAGTT AGAAGATCTG 
AGCTCGGAGT GGAAGGCGGT AACCCACTTA CTTCAAGAGC TGAGGGCAAA GTGGCCTGGC 
CCAGTCCCTG GACTGACAGC AACTGGAGCA CCTCCTAGTC AGACGGTTGC TCTGGTGACA 
CAACCTGTGG TTGCCAAGGA AACCGCTGTC TCCAAACTAG AAATGCCATC TTCCTTGCTG 
TTGGAGGTCC CTGCACTGGC AGATTTCAAC CGGGCTTGGA CAGAACTTAC CGACTGGCTG 
TCTCTGCTTG ATCGAGTTCT GAAAGCACAG AGGGTGATGG TGGGTGATCT TGAAGACATC 
AACGAGATGA TCATCAAGCA GAAGGCAACA CTGCAGGACT TGGAGCAGAG GCGCCCCCAG 
TTGGAAGAAC TCATTACTGC TGCCCAGAAT TTGAAAAACA AGACCAGCAA TCAAGAGGCC 
AGGACCATCA TTACTGATCG AATTGAAAGA ATTCAGAGTC AGTGGGATGA AGTACAGGAA 
CACCTTCAGA ACCGGAGGCA GCAGTTAAAT GAAATGTTAA AGGATTCAAC ACAATGGCTG 
GAAGCTAAGG AAGAAGCTGA GCAGGTGGTG GGGCAGGCCA GAGCCAAGCT TGAGACATGG 
AAGGAGGGTC CCTACACAAT GGATGCAATC CAAAGGAAGA TCACAGAAAC CAAGCAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTGG CAAATGACTT GGCACTGAAA 
CTTCTCCGGG ATTATTCTGC AGATGATACC CGAAAGGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT TCATAAAAGA GTGAGTGAGC GAGAGACTGC TCTGGAAGAA 
ACTCATAGAT TACTGCAACA GTTCCCCCTG GACTTGGAGA AGTTTCTTGC CTGGCTTACA 
GAAGCTGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GACTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAGG ACCTCCAAGG AGAAATTGAA 
GCTCACACAG ATATCTATCA CAACCTAGAT GAAAATGGCC AAAAAATCCT GAGATCCCTG 
GAAGGTTCTG ATGACGCGGT CCTATTACAA AGACGTTTGG ATAATATGAA TTTCAAGTGG 
AGTGAGCTGC GGAAAAAGTC TCTCAATATC AGGTCCCATT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTGT GGCTACAGCT GAAAGACGAT 
GAGTTGAGCC GTCAGGCACC TATTGGAGGC GATTTCCCAG CAGTTCAGAA GCAGAATGAT 
ATACACAGGG CCTTCAAGAG GGAACTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTGGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCGCC TGAGGAGAAA GCCCAGAATG TCACACGACT CCTACGAAAG 
CAGGCTGAGG AGGTCAACAC AGAGTGGGAA AAATTGAACC TACACTCGGC TGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAAGAACTGC AGGAGGCCAC GGACGAGCTG 
GACCTCAAAC TGCGCCAAGC TGAGGTGATC AAGGGATCAT GGCAGCCCGT GGGCGATCTT 
CTCATTGACT CCCTCCAGGA TCACCTCGAG AAAGTCAAGG CACTTCGAGG AGAAATTGCA 
CCTCTCAAAG AGAATGTAAG CCACGTCAGT GACCTTGCTC GCCAACTCAC CACGTTGGGC 
ATTCAGCTTT CACCATATAA TCTCAGCACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CTGTTGAGGA CCGCATCAGG CAGCTGCATG AAGCCCACAG GGACTTTGGC 
CCAGCGTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCT 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCGGCTTAT 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT TTTGAGCCTG 
TCAGCCGCAT GTGATGCCTT GGACCAGCAC AACCTCAAGC AAAACGACCA GCCCATGGAC 
ATCCTGCAGA TCATTAACTG TTTGACCACT ATTTATGACC GCCTGGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GATACGGGAC GAACAGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCGC ATTTGGAAGA CAAGTACAGA TACCTTTTCA AGCAAGTGGC CAGTTCAACA 
GGATTTTGTG ACCAGCGCAG ACTGGGCCTC CTTCTACATG ACTCTATCCA AATCCCAAGA 
CAGTTGGGTG AAGTTGCATC CTTTGGGGGC AGTAACATTG AGCCAAGTGT CAGGAGCTGC 
TTCCAATTCG CTAATAATAA GCCTGAGATC GAAGCAGCTC TCTTCCTCGA TTGGATGAGA 
CTAGAACCCC AGTCCATGGT GTGGCTGCCC GTCCTGCACA GGGTGGCTGC CGCGGAGACC 
GCCAAGCATC AGGCCAAGTG TAACATCTGC AAGGAGTGTC CAATCATCGG CTTCAGGTAC 
AGAAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGCT GCTTTTTCTC TGGCCGCGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATATT GTACTCCGAC TACATCGGGA 
GAAGATGTTC GAGACTTTGC CAAGGTACTA AAAAACAAAT TTCGCACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACGG TCTTAGAGGG GGACAACATG 
GAAACTCCTG TTACTCTGAT CAACTTCTGG CCGGTAGACT CTGCGCCTGC CTCGTCCCCT 
CAGCTTTCAC ACGATGATAC TCATTCACGC ATTGAACATT ATGCTAGCAG GCTAGCAGAA 
ATGGAAAACA GCAGTGGATC TTATCTAAAT GATAGCATCT CTCCTAATGA GAGCATAGAT 
GATGAACATT TGTTAATCCA GCATTACTGC CAAAGTTTGA ACCAGGACTC CCCCCTGAGC 
CAGCCTCGTA GTCCTGCCCA GATCTTGATT TCCTTAGAGA GTGAGGAAAG AGGGGAGCTA 
GAGAGAATCC TAGCAGATCT TGAGGAAGAA AACAGGAATC TGCAGGCTGA ATATGATCGT 
CTCAAGGAGC AGCATGAACA TAAAGGCCTG TCCCCACTGC CGTCCCCTCC TGAGATGATG 
CCCACCTCGC CACAGAGTCC CCGGGATGCT GAGCTCATTG CTGAGGCCAA GCTACTGCGT 
CAACACAAAG GCCGCCTGGA AGCCAGGATG CAAATCCTGG AAGACCACAA TAAACAGCTG 
GAGTCCCAGT TACACAGGCT CAGGCAGCTG CTGGAGCAAC CCCAGGCTGA GGCCAAGGTG 
AATGGCACAA CGGTGTCCTC TCCTTCTACC TCTCTTCAGA GGTCAGACAG CAGCCAGCCT 
ATGCTGCTCC GGGTGGTTGG CAGTCAAACT TCAGAATCCA TGGGCGAGGA AGACCTTCTC 
AGTCCTCCCC AGGACTCAAG CACAGGGTTA GAGGAAGTGA TGGAGCAACT CAACAACTCC 
TTCCCTAGTT CCAGAGGACA CAATGTAGGA AGCCTTTTCC ACTTGGCAGA TGATTTGGGC 
AGAGCGATGG AGTCCTTAGT TTCAGTTATG ACAGATGAAG AAGGGGCAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844126.1
CDS	251..11344
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALQVLQKNNVDLVNIGSSDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVRQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYVTSLFQVLPQQVSLEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERTPSSPQPRFKSYAYTQAAYVSTSDPTRSPFPSQRLEAPEDKSFGSPLMETEVNLDSYQTALEEVLSWLLSAEDTLQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGSGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNAWLTKTEERTRKMEKEPLGPDLEDLKRQIQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDVLLKWQRFTEEQCLFSTWLSDKEDALNKIPTSGFKDQSEMLSSLQKLAVLKTDLEKKKQTMDKLCSLNHDLLSTLKNTLVAQKMEAWLDNFAQRWDNLVQKLEKSSTQISQAVTTTQPSLTQTTVMETVTMVTTREQILVKHAQEELPPPPPQKKRQIIVDSEIKKRLDVDITELHSWITRSEAVLQSPEFAVYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTPSEPTAVKSQLKNCKDEVNRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADMQAREKELQTILDTLPTVRYQETMSTILTWIQQSETKLSIPQVTVTEYEIMEQRLEELQALQSSLQEHQNDLNYLSTTVKEMSRKAPSHISQRYQSEFENIEGRWKKLSAQLNERCQKLEEQMAKLRKLQNHIKTLKKWMAEVDVFLKDEWPALGDSEILKKQLKQCRLLVSDIQTIQPSLNSVNEGGQKIKTEAEPEFASRLETELRELNTQWDYICRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVQLLTESVNSVIAQAPPAAQEALRKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLNEVELKLKTTENIPGGAEEISEVLSSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLQLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHYQGKETAQRVLSQIEVAQKKMQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDLELTKRSAVEGMPSNLDSEVAWGKATQKETEKQKVHLKSITELGEALKTVLGKKETLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMETFDQNVDHITKWIIQADALLDESEKKKPQQKEDMLKRLKAELNDIRPKVDSTRDQAANLMANRGDHCRKVIEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVHRQAEGLSEDGAAMAVESTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVMVMTEDMPLEISYVPSTYLTEITHVLQALSEVEQLLNAPDLCAKDFQDLFKQEESLKNIKDNLQQISGRIDVIHNKKAAALQSTTPPEKARLQEALSRLDFQWERVNKMYKDRQGQFDRSVEKWRRFHYDMKIFNQWLTEAEHFLKKTQIPENWEHAKYKWYLKELQDGIGQRQTVVRVLNATGEEIIQQSSKIDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDLNEFVLWLEEAGNISSIPLEPGNEQQLKEKLEEVKLLAEELPLRQGILKQLNETGGTVLVSAPISPEEQDKLENKLKQTNLQWIKVSRSLPEKQGEIEAHIKDLGQLEEQLNHLLLWLSPIRSQLEIYNQPNQTGPFDIKETEVAVQAKQPDVEGILSKGQNLYKEKPATEPVKRKLEDLSSEWKAVTHLLQELRAKWPGPVPGLTATGAPPSQTVALVTQPVVAKETAVSKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVLKAQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVVGQARAKLETWKEGPYTMDAIQRKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRVSERETALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKILRSLEGSDDAVLLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDIHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEEKAQNVTRLLRKQAEEVNTEWEKLNLHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVSHVSDLARQLTTLGIQLSPYNLSTLEDLNTRWKLLQVAVEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSSGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKEQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDSSTGLEEVMEQLNNSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_024988358.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophin (DMD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988358.1

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAATTTTCT AAGTTCGGGA AGCAGCACAT AGAAAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTC TTGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCAGGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAATA TCGGAAGCTC TGACATAGTA 
GATGGAAATC ATAAACTGAC TCTTGGCTTG ATTTGGAATA TAATCCTCCA TTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA GAAGATACTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT CAACTTCACC 
ACCAGCTGGT CTGACGGTTT GGCTTTGAAT GCGCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TCGCCAGCAG TCAGCTACAC AACGACTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTACTGG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TACGTCACAT CTCTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCCTTGAAGC CATCCAGGAG GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACAAGAGAAG AACATTTTCA ACTACATCAT CAAATGCACT ACTCTCAACA GATCACTGTC 
AGTCTAGCAC AGGGCTATGA ACGAACTCCT TCCTCTCCTC AGCCTCGCTT CAAGAGCTAT 
GCCTACACGC AGGCTGCTTA TGTCTCCACC TCTGACCCCA CACGGAGCCC ATTTCCTTCA 
CAGCGTTTGG AAGCTCCTGA AGACAAGTCA TTTGGCAGTC CATTGATGGA GACTGAAGTA 
AACCTGGACA GTTACCAGAC AGCTTTAGAA GAAGTACTCT CATGGCTCCT TTCAGCCGAA 
GACACATTGC AAGCACAAGG AGAGATTTCT AATGATGTAG AAGAAGTGAA AGAACAGTTT 
CATACCCACG AGGGATACAT GATGGATTTG ACATCCCATC AGGGACGGGT TGGCAATGTT 
CTCCAATTGG GGAGTCAGCT GATTGGGTCA GGGAAATTAT CAGAAGATGA AGAAACTGAA 
GTGCAAGAAC AAATGAATCT TCTAAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTC ATGGATCTCC AGAATCAGCA GCTGAAAGAG 
TTAAATGCCT GGTTAACAAA AACAGAAGAG AGAACAAGGA AAATGGAAAA AGAGCCCCTT 
GGACCTGATC TTGAGGACCT CAAACGCCAA ATACAGCAAC ATAAGGTGCT TCAAGAAGAC 
CTAGAACAGG AACAAGTCAG AGTCAATTCT CTCACGCACA TGGTGGTGGT TGTAGATGAG 
TCTAGTGGTG ATCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGG 
TGGGCAAACA TCTGTAGGTG GACTGAAGAT CGCTGGGTTC TTTTACAGGA TGTTCTTCTA 
AAATGGCAAC GTTTTACTGA AGAGCAGTGC CTTTTTAGTA CATGGCTTTC AGATAAAGAA 
GATGCATTGA ACAAGATTCC CACAAGTGGC TTTAAGGATC AAAGTGAAAT GTTATCAAGT 
CTTCAAAAAC TGGCCGTTTT AAAAACTGAT CTAGAAAAGA AAAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCATGA TCTCCTTTCA ACACTGAAAA ATACATTGGT TGCCCAAAAG 
ATGGAAGCAT GGTTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA AAAACTTGAA 
AAGAGTTCAA CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACGGTAAC TATGGTGACC ACGAGGGAGC AAATCCTGGT AAAGCATGCC 
CAAGAGGAAC TCCCTCCACC ACCTCCACAA AAGAAGAGGC AGATTATTGT GGATTCTGAA 
ATTAAGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCCGTGTTAC AGAGTCCTGA ATTTGCAGTC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGTATCAAAC AAGCCTCAGA ACAACTGAAC AGCCGGTGGA TCGAGTTCTG CCAATTGCTA 
AGTGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCA GAAAACTGGT TGAAAACTCA ACCCACTACC 
CCATCAGAGC CAACAGCAGT TAAAAGCCAG TTAAAAAATT GTAAGGATGA AGTCAACCGG 
CTGTCAGCTC TTCAGCCTCA AATCGAGAGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCC GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATATGCA GGCCAGAGAG AAAGAGTTAC AGACAATTTT GGACACTTTA 
CCCACCGTGC GGTATCAGGA GACCATGAGT ACCATCCTCA CGTGGATCCA ACAGTCAGAA 
ACCAAACTCT CGATACCTCA GGTTACTGTC ACTGAATACG AGATCATGGA GCAGAGACTT 
GAGGAGTTAC AGGCTTTACA AAGCTCTCTG CAAGAGCACC AAAATGACCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAGGAAA GCACCCTCTC ATATAAGCCA GAGATATCAG 
TCAGAATTTG AAAACATTGA GGGTCGGTGG AAGAAACTCT CTGCCCAGCT GAATGAACGT 
TGCCAAAAGC TAGAGGAGCA AATGGCCAAA CTCCGAAAAC TTCAGAATCA CATAAAAACA 
CTGAAGAAGT GGATGGCTGA AGTTGATGTT TTCTTGAAAG ATGAATGGCC TGCCCTTGGA 
GATTCAGAAA TTCTTAAAAA ACAGCTGAAG CAGTGCAGGC TTTTAGTCAG TGATATTCAG 
ACAATCCAGC CCAGTCTAAA CAGTGTCAAT GAAGGTGGAC AGAAAATAAA AACTGAAGCG 
GAGCCAGAGT TTGCTTCTAG ACTGGAGACA GAGCTCAGAG AACTTAACAC TCAATGGGAT 
TACATATGTC GACAGGTCTA TGCCAGAAAG GAGGCCTTAA AGGGAGGCTT GGATAAAACT 
GTAAGCCTTC AGAAAGATTT GTCAGAGATG CATGAATGGA TGACACAAGC CGAAGAAGAA 
TACCTAGAGA GAGATTTTGA ATATAAAACC CCAGATGAAT TACAGACAGC AGTTGAAGAG 
ATGAAGAGAG CTAAAGAAGA GGCCCAGCAA AAAGAAGCAA AAGTGCAGCT CCTCACTGAG 
TCCGTGAACA GTGTCATAGC TCAAGCTCCA CCTGCAGCAC AAGAGGCCTT AAGAAAGGAA 
CTGGACACTC TGACCACCAA CTACCAGTGG CTCTGCACCA GGCTGAATGG AAAATGCAAG 
ACGTTAGAAG AGGTTTGGGC ATGTTGGCAT GAGTTATTGT CATACTTGGA AAAGGCAAAT 
AAGTGGCTAA ATGAAGTAGA ATTGAAACTT AAGACTACTG AAAATATTCC TGGTGGAGCT 
GAGGAAATCT CTGAGGTGCT CAGTTCACTT GAAAATTTGA TGCAACATTC AGAAGATAAC 
CCGAATCAGA TTCGCATATT GGCACAGACC CTAACAGATG GTGGAGTCAT GGATGAACTG 
ATCAATGAAG AACTCGAGAC ATTTAATTCT CGTTGGAGAG AACTACATGA AGAGGCTGTG 
AGGAGGCAAA AGTTGCTTGA ACAGAGTATC CAGTCTGCCC AGGAGATTGA AAAATCCTTA 
CAGTTAATTC AGGAATCCCT CTCATCAATT GACAAGCAGT TGGCAGCTTA TATCGCTGAC 
AAAGTGGACG CCGCTCAGAT GCCTCAGGAA GCCCAGAAAA TCCAATCTGA TTTGACAAGT 
CATGAGATCA GTTTAGAAGA AATGAAGAAA CATTACCAAG GAAAGGAAAC TGCTCAACGG 
GTACTATCCC AAATTGAAGT GGCACAGAAA AAAATGCAAG ATGTTTCCAT GAAATTTCGA 
TTGTTCCAGA AGCCAGCCAA TTTTGAGCAG CGTCTACAGG AAAGTAAGAT GATTTTAGAT 
GAAGTGAAGA TGCACTTGCC CGCACTGGAA ACAAAGAGTG TTGAACAGGA AGTAGTACAG 
TCACAGTTAA ATCATTGTGT GAACTTGTAT AAAAGTCTGA GTGAAGTGAA GTCTGAAGTG 
GAAATGGTGA TTAAAACTGG ACGTCAAATT GTACAGAAGA AGCAGACGGA AAACCCTAAA 
GAGCTTGATG AAAGAGTAAC GGCTTTGAAA TTGCATTATA ATGAGCTGGG AGCAAAGGTA 
ACAGAAAGAA AGCAACAGTT GGAGAAATGC TTGAAATTGT CCCGTAAGAT GCGAAAGGAA 
ATGAATGCCT TGACAGAATG GCTGGCAGCT ACAGATTTGG AACTGACAAA GAGATCAGCA 
GTTGAAGGAA TGCCTAGTAA TTTGGATTCT GAAGTTGCCT GGGGAAAGGC TACTCAGAAA 
GAGACTGAGA AACAGAAGGT TCACCTAAAG AGCATTACAG AGCTAGGAGA GGCCTTGAAA 
ACGGTTTTGG GCAAGAAGGA GACCTTGGTC GAAGATAAAC TGAGTCTTTT GAATAGTAAC 
TGGATAGCCG TCACTTCTCG AGCAGAAGAG TGGCTAAACC TTTTGTTGGA ATACCAGAAA 
CACATGGAAA CCTTTGACCA GAATGTGGAT CATATCACAA AGTGGATCAT TCAAGCTGAT 
GCACTTCTGG ATGAATCTGA GAAAAAGAAA CCACAGCAAA AAGAAGACAT GCTAAAGCGC 
TTAAAGGCTG AACTGAATGA CATACGCCCA AAGGTGGACT CCACGCGTGA CCAAGCAGCA 
AACTTGATGG CAAACCGCGG CGACCACTGC AGGAAAGTAA TAGAGCCCAA AATCTCAGAG 
CTCAACCATC GATTTGCAGC CATTTCTCAC AGAATTAAGA CTGGAAAGGC CTCCATTCCT 
TTGAAGGAAT TGGAGCAGTT TAACTCAGAT ATACAAAAAT TGCTTGAACC ACTGGAGGCT 
GAAATTCAGC AGGGGGTGAA TCTGAAAGAG GAAGACTTCA ATAAAGATAT GAGTGAAGAC 
AATGAGGGTA CTGTAAAAGA ATTGTTGCAA AGAGGAGACA ACTTACAACA AAGAATCACA 
GATGAGAGAA AGCGAGAGGA AATAAAGATA AAACAGCAGC TGTTACAGAC AAAACATAAT 
GCTCTCAAGG ATTTGAGGTC TCAAAGAAGA AAAAAGGCTC TAGAAATTTC TCACCAGTGG 
TATCAGTACA AGAGGCAGGC TGATGATCTC CTGAAATGCT TGGATGACAT TGAAAAAAAA 
TTAGCCAGCC TACCTGAACC CAGAGATGAA AGGAAAATAA AGGAAATTGA TCGTGAGTTG 
CAGAAGAAGA AAGAGGAGCT GAATGCAGTG CATAGGCAAG CTGAGGGCTT GTCTGAGGAT 
GGGGCAGCAA TGGCAGTGGA GTCAACTCAG ATCCAGCTCA GCAAGCGCTG GCGGGAAATT 
GAGAGCAAAT TTGCTCAGTT TCGAAGACTC AACTTTGCAC AAATTCACAC TGTCCATGAA 
GAATCAGTGA TGGTGATGAC CGAAGACATG CCTTTGGAAA TTTCTTATGT GCCTTCTACT 
TACCTGACTG AGATCACTCA CGTCTTACAA GCCCTTTCAG AAGTGGAACA ACTTCTCAAT 
GCTCCCGACC TCTGTGCTAA AGATTTTCAA GATCTCTTCA AACAAGAGGA ATCTCTGAAG 
AATATAAAAG ACAACCTGCA ACAAATCTCA GGTCGGATTG ATGTTATTCA CAATAAGAAG 
GCGGCAGCAT TGCAAAGCAC CACACCCCCA GAAAAGGCAA GGTTACAGGA AGCTCTCTCC 
AGGCTCGACT TCCAATGGGA AAGAGTGAAC AAAATGTACA AGGACCGACA AGGGCAATTT 
GACAGATCTG TTGAAAAATG GCGACGGTTT CATTATGATA TGAAGATATT TAATCAATGG 
CTAACAGAAG CTGAACACTT TCTCAAAAAG ACACAAATTC CTGAGAACTG GGAACATGCC 
AAATATAAAT GGTATCTTAA GGAACTCCAG GATGGCATCG GGCAGCGGCA AACTGTTGTC 
AGAGTATTGA ATGCAACTGG GGAAGAAATA ATTCAGCAGT CCTCAAAAAT AGATGCCAGT 
ATTCTACAAG AAAAATTGGG AAGCCTGAAT CTGCGGTGGC AGGAGGTCTG CAAACAGCTG 
GCCGAGAGAA AAAAGAGGTT AGAAGAACAA AAGAATATCC TGTCAGAATT TCAAAGAGAT 
TTGAATGAAT TTGTTTTATG GTTGGAAGAA GCAGGTAACA TTTCTAGTAT TCCTCTTGAA 
CCTGGAAATG AGCAGCAACT AAAAGAAAAG CTTGAGGAAG TCAAGTTACT GGCCGAAGAG 
TTGCCCCTGC GTCAGGGAAT TCTAAAACAA TTAAATGAAA CAGGAGGAAC AGTGCTTGTA 
AGTGCTCCCA TAAGCCCAGA AGAGCAAGAT AAACTTGAAA ATAAGCTCAA GCAGACAAAT 
CTTCAGTGGA TAAAGGTTTC TAGGAGTTTA CCTGAGAAAC AGGGAGAAAT TGAGGCTCAC 
ATAAAAGACC TTGGACAGCT TGAAGAGCAA TTAAATCATC TGCTTCTGTG GCTGTCTCCT 
ATTAGGAGTC AGTTGGAAAT TTACAACCAA CCAAATCAGA CAGGACCATT TGACATTAAG 
GAAACTGAAG TAGCAGTTCA AGCTAAACAG CCGGATGTGG AAGGGATTTT GTCTAAAGGG 
CAGAATTTGT ACAAGGAAAA ACCAGCCACT GAGCCAGTGA AGAGGAAGTT AGAAGATCTG 
AGCTCGGAGT GGAAGGCGGT AACCCACTTA CTTCAAGAGC TGAGGGCAAA GTGGCCTGGC 
CCAGTCCCTG GACTGACAGC AACTGGAGCA CCTCCTAGTC AGACGGTTGC TCTGGTGACA 
CAACCTGTGG TTGCCAAGGA AACCGCTGTC TCCAAACTAG AAATGCCATC TTCCTTGCTG 
TTGGAGGTCC CTGCACTGGC AGATTTCAAC CGGGCTTGGA CAGAACTTAC CGACTGGCTG 
TCTCTGCTTG ATCGAGTTCT GAAAGCACAG AGGGTGATGG TGGGTGATCT TGAAGACATC 
AACGAGATGA TCATCAAGCA GAAGGCAACA CTGCAGGACT TGGAGCAGAG GCGCCCCCAG 
TTGGAAGAAC TCATTACTGC TGCCCAGAAT TTGAAAAACA AGACCAGCAA TCAAGAGGCC 
AGGACCATCA TTACTGATCG AATTGAAAGA ATTCAGAGTC AGTGGGATGA AGTACAGGAA 
CACCTTCAGA ACCGGAGGCA GCAGTTAAAT GAAATGTTAA AGGATTCAAC ACAATGGCTG 
GAAGCTAAGG AAGAAGCTGA GCAGGTGGTG GGGCAGGCCA GAGCCAAGCT TGAGACATGG 
AAGGAGGGTC CCTACACAAT GGATGCAATC CAAAGGAAGA TCACAGAAAC CAAGCAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTGG CAAATGACTT GGCACTGAAA 
CTTCTCCGGG ATTATTCTGC AGATGATACC CGAAAGGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT TCATAAAAGA GTGAGTGAGC GAGAGACTGC TCTGGAAGAA 
ACTCATAGAT TACTGCAACA GTTCCCCCTG GACTTGGAGA AGTTTCTTGC CTGGCTTACA 
GAAGCTGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GACTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAGG ACCTCCAAGG AGAAATTGAA 
GCTCACACAG ATATCTATCA CAACCTAGAT GAAAATGGCC AAAAAATCCT GAGATCCCTG 
GAAGGTTCTG ATGACGCGGT CCTATTACAA AGACGTTTGG ATAATATGAA TTTCAAGTGG 
AGTGAGCTGC GGAAAAAGTC TCTCAATATC AGGTCCCATT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTGT GGCTACAGCT GAAAGACGAT 
GAGTTGAGCC GTCAGGCACC TATTGGAGGC GATTTCCCAG CAGTTCAGAA GCAGAATGAT 
ATACACAGGG CCTTCAAGAG GGAACTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTGGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCGCC TGAGGAGAAA GCCCAGAATG TCACACGACT CCTACGAAAG 
CAGGCTGAGG AGGTCAACAC AGAGTGGGAA AAATTGAACC TACACTCGGC TGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAAGAACTGC AGGAGGCCAC GGACGAGCTG 
GACCTCAAAC TGCGCCAAGC TGAGGTGATC AAGGGATCAT GGCAGCCCGT GGGCGATCTT 
CTCATTGACT CCCTCCAGGA TCACCTCGAG AAAGTCAAGG CACTTCGAGG AGAAATTGCA 
CCTCTCAAAG AGAATGTAAG CCACGTCAGT GACCTTGCTC GCCAACTCAC CACGTTGGGC 
ATTCAGCTTT CACCATATAA TCTCAGCACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CTGTTGAGGA CCGCATCAGG CAGCTGCATG AAGCCCACAG GGACTTTGGC 
CCAGCGTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCT 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCGGCTTAT 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT TTTGAGCCTG 
TCAGCCGCAT GTGATGCCTT GGACCAGCAC AACCTCAAGC AAAACGACCA GCCCATGGAC 
ATCCTGCAGA TCATTAACTG TTTGACCACT ATTTATGACC GCCTGGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GATACGGGAC GAACAGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCGC ATTTGGAAGA CAAGTACAGA TACCTTTTCA AGCAAGTGGC CAGTTCAACA 
GGATTTTGTG ACCAGCGCAG ACTGGGCCTC CTTCTACATG ACTCTATCCA AATCCCAAGA 
CAGTTGGGTG AAGTTGCATC CTTTGGGGGC AGTAACATTG AGCCAAGTGT CAGGAGCTGC 
TTCCAATTCG CTAATAATAA GCCTGAGATC GAAGCAGCTC TCTTCCTCGA TTGGATGAGA 
CTAGAACCCC AGTCCATGGT GTGGCTGCCC GTCCTGCACA GGGTGGCTGC CGCGGAGACC 
GCCAAGCATC AGGCCAAGTG TAACATCTGC AAGGAGTGTC CAATCATCGG CTTCAGGTAC 
AGAAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGCT GCTTTTTCTC TGGCCGCGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATATT GTACTCCGAC TACATCGGGA 
GAAGATGTTC GAGACTTTGC CAAGGTACTA AAAAACAAAT TTCGCACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACGG TCTTAGAGGG GGACAACATG 
GAAACTCCTG TTACTCTGAT CAACTTCTGG CCGGTAGACT CTGCGCCTGC CTCGTCCCCT 
CAGCTTTCAC ACGATGATAC TCATTCACGC ATTGAACATT ATGCTAGCAG GCTAGCAGAA 
ATGGAAAACA GCAGTGGATC TTATCTAAAT GATAGCATCT CTCCTAATGA GAGCATAGAT 
GATGAACATT TGTTAATCCA GCATTACTGC CAAAGTTTGA ACCAGGACTC CCCCCTGAGC 
CAGCCTCGTA GTCCTGCCCA GATCTTGATT TCCTTAGAGA GTGAGGAAAG AGGGGAGCTA 
GAGAGAATCC TAGCAGATCT TGAGGAAGAA AACAGGAATC TGCAGGCTGA ATATGATCGT 
CTCAAGGAGC AGCATGAACA TAAAGGCCTG TCCCCACTGC CGTCCCCTCC TGAGATGATG 
CCCACCTCGC CACAGAGTCC CCGGGATGCT GAGCTCATTG CTGAGGCCAA GCTACTGCGT 
CAACACAAAG GCCGCCTGGA AGCCAGGATG CAAATCCTGG AAGACCACAA TAAACAGCTG 
GAGTCCCAGT TACACAGGCT CAGGCAGCTG CTGGAGCAAC CCCAGGCTGA GGCCAAGGTG 
AATGGCACAA CGGTGTCCTC TCCTTCTACC TCTCTTCAGA GGTCAGACAG CAGCCAGCCT 
ATGCTGCTCC GGGTGGTTGG CAGTCAAACT TCAGAATCCA TGGGCGAGGA AGACCTTCTC 
AGTCCTCCCC AGGACTCAAG CACAGGGTTA GAGGAAGTGA TGGAGCAACT CAACAACTCC 
TTCCCTAGTT CCAGAGGACA CAATGTAGGA AGCCTTTTCC ACTTGGCAGA TGATTTGGGC 
AGAGCGATGG AGTCCTTAGT TTCAGTTATG ACAGATGAAG AAGGGGCAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_024988359.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844127.1 Bos taurus dystrophin (DMD), transcript variant X2, mRNA.

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CloneID	OBa238538
Clone ID Related Accession (Same CDS sequence)	XM_024988359.1
Accession Version	XM_024988359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATG AAAGAGAAGA TGTTCAAAAG AAAACATTCA CAAAATGGAT AAATGCACAA 
TTTTCTAAGT TCGGGAAGCA GCACATAGAA AACCTCTTCA GTGACCTACA GGATGGGAGA 
CGCCTCCTAG ACCTTCTTGA AGGCCTGACA GGGCAAAAAC TGCCAAAAGA AAAAGGATCC 
ACAAGAGTTC ATGCCCTGAA CAATGTCAAC AAGGCACTGC AGGTCTTGCA GAAAAATAAT 
GTTGATTTAG TGAATATCGG AAGCTCTGAC ATAGTAGATG GAAATCATAA ACTGACTCTT 
GGCTTGATTT GGAATATAAT CCTCCATTGG CAGGTCAAAA ATGTAATGAA AAATATCATG 
GCTGGATTGC AACAAACCAA CAGTGAGAAG ATACTCCTGA GCTGGGTCCG ACAATCAACT 
CGTAATTATC CACAGGTTAA TGTCATCAAC TTCACCACCA GCTGGTCTGA CGGTTTGGCT 
TTGAATGCGC TCATCCACAG TCATAGGCCA GACCTGTTTG ATTGGAATAG TGTGGTTCGC 
CAGCAGTCAG CTACACAACG ACTGGAACAT GCATTCAACA TTGCCAAATA TCAATTAGGC 
ATAGAGAAAC TACTGGATCC TGAAGATGTT GCCACCACTT ATCCAGATAA GAAGTCCATC 
TTAATGTACG TCACATCTCT CTTCCAAGTT TTGCCTCAAC AAGTGAGCCT TGAAGCCATC 
CAGGAGGTGG AAATGTTGCC AAGGCCATCA AAAGTTACAA GAGAAGAACA TTTTCAACTA 
CATCATCAAA TGCACTACTC TCAACAGATC ACTGTCAGTC TAGCACAGGG CTATGAACGA 
ACTCCTTCCT CTCCTCAGCC TCGCTTCAAG AGCTATGCCT ACACGCAGGC TGCTTATGTC 
TCCACCTCTG ACCCCACACG GAGCCCATTT CCTTCACAGC GTTTGGAAGC TCCTGAAGAC 
AAGTCATTTG GCAGTCCATT GATGGAGACT GAAGTAAACC TGGACAGTTA CCAGACAGCT 
TTAGAAGAAG TACTCTCATG GCTCCTTTCA GCCGAAGACA CATTGCAAGC ACAAGGAGAG 
ATTTCTAATG ATGTAGAAGA AGTGAAAGAA CAGTTTCATA CCCACGAGGG ATACATGATG 
GATTTGACAT CCCATCAGGG ACGGGTTGGC AATGTTCTCC AATTGGGGAG TCAGCTGATT 
GGGTCAGGGA AATTATCAGA AGATGAAGAA ACTGAAGTGC AAGAACAAAT GAATCTTCTA 
AATTCAAGAT GGGAATGCCT CAGGGTAGCT AGCATGGAAA AACAAAGCAA TTTACATAAA 
GTTCTCATGG ATCTCCAGAA TCAGCAGCTG AAAGAGTTAA ATGCCTGGTT AACAAAAACA 
GAAGAGAGAA CAAGGAAAAT GGAAAAAGAG CCCCTTGGAC CTGATCTTGA GGACCTCAAA 
CGCCAAATAC AGCAACATAA GGTGCTTCAA GAAGACCTAG AACAGGAACA AGTCAGAGTC 
AATTCTCTCA CGCACATGGT GGTGGTTGTA GATGAGTCTA GTGGTGATCA TGCAACTGCT 
GCTTTGGAAG AACAACTTAA GGTACTGGGA GATCGGTGGG CAAACATCTG TAGGTGGACT 
GAAGATCGCT GGGTTCTTTT ACAGGATGTT CTTCTAAAAT GGCAACGTTT TACTGAAGAG 
CAGTGCCTTT TTAGTACATG GCTTTCAGAT AAAGAAGATG CATTGAACAA GATTCCCACA 
AGTGGCTTTA AGGATCAAAG TGAAATGTTA TCAAGTCTTC AAAAACTGGC CGTTTTAAAA 
ACTGATCTAG AAAAGAAAAA GCAAACCATG GACAAACTCT GCTCACTCAA CCATGATCTC 
CTTTCAACAC TGAAAAATAC ATTGGTTGCC CAAAAGATGG AAGCATGGTT GGACAACTTT 
GCCCAGCGCT GGGATAATTT AGTCCAAAAA CTTGAAAAGA GTTCAACACA GATTTCACAG 
GCTGTCACCA CCACTCAGCC ATCACTAACA CAGACAACTG TAATGGAAAC GGTAACTATG 
GTGACCACGA GGGAGCAAAT CCTGGTAAAG CATGCCCAAG AGGAACTCCC TCCACCACCT 
CCACAAAAGA AGAGGCAGAT TATTGTGGAT TCTGAAATTA AGAAAAGGTT GGATGTCGAT 
ATAACTGAAC TTCACAGTTG GATTACTCGT TCAGAAGCCG TGTTACAGAG TCCTGAATTT 
GCAGTCTATC GGAAGGAAGG CAACTTCTCA GACCTTAAAG AAAAAGTCAA TGCCATAGAG 
CGAGAAAAAG CCGAGAAGTT CAGAAAACTG CAAGATGCCA GCAGATCAGC TCAGGCCCTG 
GTGGAACAGA TGGTGAATGA GGGTGTTAAT GCTGACAGTA TCAAACAAGC CTCAGAACAA 
CTGAACAGCC GGTGGATCGA GTTCTGCCAA TTGCTAAGTG AGAGACTTAA CTGGCTGGAG 
TATCAGAACA ACATCATCAC TTTCTATAAT CAGCTACAAC AATTGGAGCA GATGACAACT 
ACTGCAGAAA ACTGGTTGAA AACTCAACCC ACTACCCCAT CAGAGCCAAC AGCAGTTAAA 
AGCCAGTTAA AAAATTGTAA GGATGAAGTC AACCGGCTGT CAGCTCTTCA GCCTCAAATC 
GAGAGATTAA AAATTCAAAG CATAGCCCTG AAAGAGAAAG GACAAGGGCC AATGTTCCTG 
GATGCCGACT TTGTGGCCTT TACAAATCAT TTTAACCAAG TCTTTGCTGA TATGCAGGCC 
AGAGAGAAAG AGTTACAGAC AATTTTGGAC ACTTTACCCA CCGTGCGGTA TCAGGAGACC 
ATGAGTACCA TCCTCACGTG GATCCAACAG TCAGAAACCA AACTCTCGAT ACCTCAGGTT 
ACTGTCACTG AATACGAGAT CATGGAGCAG AGACTTGAGG AGTTACAGGC TTTACAAAGC 
TCTCTGCAAG AGCACCAAAA TGACCTAAAC TATCTCAGCA CCACTGTGAA AGAGATGTCA 
AGGAAAGCAC CCTCTCATAT AAGCCAGAGA TATCAGTCAG AATTTGAAAA CATTGAGGGT 
CGGTGGAAGA AACTCTCTGC CCAGCTGAAT GAACGTTGCC AAAAGCTAGA GGAGCAAATG 
GCCAAACTCC GAAAACTTCA GAATCACATA AAAACACTGA AGAAGTGGAT GGCTGAAGTT 
GATGTTTTCT TGAAAGATGA ATGGCCTGCC CTTGGAGATT CAGAAATTCT TAAAAAACAG 
CTGAAGCAGT GCAGGCTTTT AGTCAGTGAT ATTCAGACAA TCCAGCCCAG TCTAAACAGT 
GTCAATGAAG GTGGACAGAA AATAAAAACT GAAGCGGAGC CAGAGTTTGC TTCTAGACTG 
GAGACAGAGC TCAGAGAACT TAACACTCAA TGGGATTACA TATGTCGACA GGTCTATGCC 
AGAAAGGAGG CCTTAAAGGG AGGCTTGGAT AAAACTGTAA GCCTTCAGAA AGATTTGTCA 
GAGATGCATG AATGGATGAC ACAAGCCGAA GAAGAATACC TAGAGAGAGA TTTTGAATAT 
AAAACCCCAG ATGAATTACA GACAGCAGTT GAAGAGATGA AGAGAGCTAA AGAAGAGGCC 
CAGCAAAAAG AAGCAAAAGT GCAGCTCCTC ACTGAGTCCG TGAACAGTGT CATAGCTCAA 
GCTCCACCTG CAGCACAAGA GGCCTTAAGA AAGGAACTGG ACACTCTGAC CACCAACTAC 
CAGTGGCTCT GCACCAGGCT GAATGGAAAA TGCAAGACGT TAGAAGAGGT TTGGGCATGT 
TGGCATGAGT TATTGTCATA CTTGGAAAAG GCAAATAAGT GGCTAAATGA AGTAGAATTG 
AAACTTAAGA CTACTGAAAA TATTCCTGGT GGAGCTGAGG AAATCTCTGA GGTGCTCAGT 
TCACTTGAAA ATTTGATGCA ACATTCAGAA GATAACCCGA ATCAGATTCG CATATTGGCA 
CAGACCCTAA CAGATGGTGG AGTCATGGAT GAACTGATCA ATGAAGAACT CGAGACATTT 
AATTCTCGTT GGAGAGAACT ACATGAAGAG GCTGTGAGGA GGCAAAAGTT GCTTGAACAG 
AGTATCCAGT CTGCCCAGGA GATTGAAAAA TCCTTACAGT TAATTCAGGA ATCCCTCTCA 
TCAATTGACA AGCAGTTGGC AGCTTATATC GCTGACAAAG TGGACGCCGC TCAGATGCCT 
CAGGAAGCCC AGAAAATCCA ATCTGATTTG ACAAGTCATG AGATCAGTTT AGAAGAAATG 
AAGAAACATT ACCAAGGAAA GGAAACTGCT CAACGGGTAC TATCCCAAAT TGAAGTGGCA 
CAGAAAAAAA TGCAAGATGT TTCCATGAAA TTTCGATTGT TCCAGAAGCC AGCCAATTTT 
GAGCAGCGTC TACAGGAAAG TAAGATGATT TTAGATGAAG TGAAGATGCA CTTGCCCGCA 
CTGGAAACAA AGAGTGTTGA ACAGGAAGTA GTACAGTCAC AGTTAAATCA TTGTGTGAAC 
TTGTATAAAA GTCTGAGTGA AGTGAAGTCT GAAGTGGAAA TGGTGATTAA AACTGGACGT 
CAAATTGTAC AGAAGAAGCA GACGGAAAAC CCTAAAGAGC TTGATGAAAG AGTAACGGCT 
TTGAAATTGC ATTATAATGA GCTGGGAGCA AAGGTAACAG AAAGAAAGCA ACAGTTGGAG 
AAATGCTTGA AATTGTCCCG TAAGATGCGA AAGGAAATGA ATGCCTTGAC AGAATGGCTG 
GCAGCTACAG ATTTGGAACT GACAAAGAGA TCAGCAGTTG AAGGAATGCC TAGTAATTTG 
GATTCTGAAG TTGCCTGGGG AAAGGCTACT CAGAAAGAGA CTGAGAAACA GAAGGTTCAC 
CTAAAGAGCA TTACAGAGCT AGGAGAGGCC TTGAAAACGG TTTTGGGCAA GAAGGAGACC 
TTGGTCGAAG ATAAACTGAG TCTTTTGAAT AGTAACTGGA TAGCCGTCAC TTCTCGAGCA 
GAAGAGTGGC TAAACCTTTT GTTGGAATAC CAGAAACACA TGGAAACCTT TGACCAGAAT 
GTGGATCATA TCACAAAGTG GATCATTCAA GCTGATGCAC TTCTGGATGA ATCTGAGAAA 
AAGAAACCAC AGCAAAAAGA AGACATGCTA AAGCGCTTAA AGGCTGAACT GAATGACATA 
CGCCCAAAGG TGGACTCCAC GCGTGACCAA GCAGCAAACT TGATGGCAAA CCGCGGCGAC 
CACTGCAGGA AAGTAATAGA GCCCAAAATC TCAGAGCTCA ACCATCGATT TGCAGCCATT 
TCTCACAGAA TTAAGACTGG AAAGGCCTCC ATTCCTTTGA AGGAATTGGA GCAGTTTAAC 
TCAGATATAC AAAAATTGCT TGAACCACTG GAGGCTGAAA TTCAGCAGGG GGTGAATCTG 
AAAGAGGAAG ACTTCAATAA AGATATGAGT GAAGACAATG AGGGTACTGT AAAAGAATTG 
TTGCAAAGAG GAGACAACTT ACAACAAAGA ATCACAGATG AGAGAAAGCG AGAGGAAATA 
AAGATAAAAC AGCAGCTGTT ACAGACAAAA CATAATGCTC TCAAGGATTT GAGGTCTCAA 
AGAAGAAAAA AGGCTCTAGA AATTTCTCAC CAGTGGTATC AGTACAAGAG GCAGGCTGAT 
GATCTCCTGA AATGCTTGGA TGACATTGAA AAAAAATTAG CCAGCCTACC TGAACCCAGA 
GATGAAAGGA AAATAAAGGA AATTGATCGT GAGTTGCAGA AGAAGAAAGA GGAGCTGAAT 
GCAGTGCATA GGCAAGCTGA GGGCTTGTCT GAGGATGGGG CAGCAATGGC AGTGGAGTCA 
ACTCAGATCC AGCTCAGCAA GCGCTGGCGG GAAATTGAGA GCAAATTTGC TCAGTTTCGA 
AGACTCAACT TTGCACAAAT TCACACTGTC CATGAAGAAT CAGTGATGGT GATGACCGAA 
GACATGCCTT TGGAAATTTC TTATGTGCCT TCTACTTACC TGACTGAGAT CACTCACGTC 
TTACAAGCCC TTTCAGAAGT GGAACAACTT CTCAATGCTC CCGACCTCTG TGCTAAAGAT 
TTTCAAGATC TCTTCAAACA AGAGGAATCT CTGAAGAATA TAAAAGACAA CCTGCAACAA 
ATCTCAGGTC GGATTGATGT TATTCACAAT AAGAAGGCGG CAGCATTGCA AAGCACCACA 
CCCCCAGAAA AGGCAAGGTT ACAGGAAGCT CTCTCCAGGC TCGACTTCCA ATGGGAAAGA 
GTGAACAAAA TGTACAAGGA CCGACAAGGG CAATTTGACA GATCTGTTGA AAAATGGCGA 
CGGTTTCATT ATGATATGAA GATATTTAAT CAATGGCTAA CAGAAGCTGA ACACTTTCTC 
AAAAAGACAC AAATTCCTGA GAACTGGGAA CATGCCAAAT ATAAATGGTA TCTTAAGGAA 
CTCCAGGATG GCATCGGGCA GCGGCAAACT GTTGTCAGAG TATTGAATGC AACTGGGGAA 
GAAATAATTC AGCAGTCCTC AAAAATAGAT GCCAGTATTC TACAAGAAAA ATTGGGAAGC 
CTGAATCTGC GGTGGCAGGA GGTCTGCAAA CAGCTGGCCG AGAGAAAAAA GAGGTTAGAA 
GAACAAAAGA ATATCCTGTC AGAATTTCAA AGAGATTTGA ATGAATTTGT TTTATGGTTG 
GAAGAAGCAG GTAACATTTC TAGTATTCCT CTTGAACCTG GAAATGAGCA GCAACTAAAA 
GAAAAGCTTG AGGAAGTCAA GTTACTGGCC GAAGAGTTGC CCCTGCGTCA GGGAATTCTA 
AAACAATTAA ATGAAACAGG AGGAACAGTG CTTGTAAGTG CTCCCATAAG CCCAGAAGAG 
CAAGATAAAC TTGAAAATAA GCTCAAGCAG ACAAATCTTC AGTGGATAAA GGTTTCTAGG 
AGTTTACCTG AGAAACAGGG AGAAATTGAG GCTCACATAA AAGACCTTGG ACAGCTTGAA 
GAGCAATTAA ATCATCTGCT TCTGTGGCTG TCTCCTATTA GGAGTCAGTT GGAAATTTAC 
AACCAACCAA ATCAGACAGG ACCATTTGAC ATTAAGGAAA CTGAAGTAGC AGTTCAAGCT 
AAACAGCCGG ATGTGGAAGG GATTTTGTCT AAAGGGCAGA ATTTGTACAA GGAAAAACCA 
GCCACTGAGC CAGTGAAGAG GAAGTTAGAA GATCTGAGCT CGGAGTGGAA GGCGGTAACC 
CACTTACTTC AAGAGCTGAG GGCAAAGTGG CCTGGCCCAG TCCCTGGACT GACAGCAACT 
GGAGCACCTC CTAGTCAGAC GGTTGCTCTG GTGACACAAC CTGTGGTTGC CAAGGAAACC 
GCTGTCTCCA AACTAGAAAT GCCATCTTCC TTGCTGTTGG AGGTCCCTGC ACTGGCAGAT 
TTCAACCGGG CTTGGACAGA ACTTACCGAC TGGCTGTCTC TGCTTGATCG AGTTCTGAAA 
GCACAGAGGG TGATGGTGGG TGATCTTGAA GACATCAACG AGATGATCAT CAAGCAGAAG 
GCAACACTGC AGGACTTGGA GCAGAGGCGC CCCCAGTTGG AAGAACTCAT TACTGCTGCC 
CAGAATTTGA AAAACAAGAC CAGCAATCAA GAGGCCAGGA CCATCATTAC TGATCGAATT 
GAAAGAATTC AGAGTCAGTG GGATGAAGTA CAGGAACACC TTCAGAACCG GAGGCAGCAG 
TTAAATGAAA TGTTAAAGGA TTCAACACAA TGGCTGGAAG CTAAGGAAGA AGCTGAGCAG 
GTGGTGGGGC AGGCCAGAGC CAAGCTTGAG ACATGGAAGG AGGGTCCCTA CACAATGGAT 
GCAATCCAAA GGAAGATCAC AGAAACCAAG CAGTTGGCCA AAGACCTCCG CCAGTGGCAG 
ATAAATGTAG ATGTGGCAAA TGACTTGGCA CTGAAACTTC TCCGGGATTA TTCTGCAGAT 
GATACCCGAA AGGTACACAT GATAACAGAG AACATCAATG CCTCTTGGGC AAGCATTCAT 
AAAAGAGTGA GTGAGCGAGA GACTGCTCTG GAAGAAACTC ATAGATTACT GCAACAGTTC 
CCCCTGGACT TGGAGAAGTT TCTTGCCTGG CTTACAGAAG CTGAAACAAC TGCCAACGTC 
CTGCAGGATG CCACCCATAA GGAAAGGCTT CTAGAAGACT CCAAGGGAGT AAGAGAGCTG 
ATGAAACAAT GGCAGGACCT CCAAGGAGAA ATTGAAGCTC ACACAGATAT CTATCACAAC 
CTAGATGAAA ATGGCCAAAA AATCCTGAGA TCCCTGGAAG GTTCTGATGA CGCGGTCCTA 
TTACAAAGAC GTTTGGATAA TATGAATTTC AAGTGGAGTG AGCTGCGGAA AAAGTCTCTC 
AATATCAGGT CCCATTTGGA AGCCAGTTCT GACCAGTGGA AGCGTCTGCA CCTTTCTCTT 
CAGGAACTTC TGGTGTGGCT ACAGCTGAAA GACGATGAGT TGAGCCGTCA GGCACCTATT 
GGAGGCGATT TCCCAGCAGT TCAGAAGCAG AATGATATAC ACAGGGCCTT CAAGAGGGAA 
CTGAAAACGA AAGAACCTGT AATCATGAGT ACTCTTGAGA CTGTACGAAT ATTTCTGACA 
GAGCAGCCTT TGGAAGGACT AGAGAAACTC TACCAGGAGC CCAGAGAGCT GCCGCCTGAG 
GAGAAAGCCC AGAATGTCAC ACGACTCCTA CGAAAGCAGG CTGAGGAGGT CAACACAGAG 
TGGGAAAAAT TGAACCTACA CTCGGCTGAC TGGCAGAGAA AAATAGACGA GGCCCTCGAA 
AGACTCCAAG AACTGCAGGA GGCCACGGAC GAGCTGGACC TCAAACTGCG CCAAGCTGAG 
GTGATCAAGG GATCATGGCA GCCCGTGGGC GATCTTCTCA TTGACTCCCT CCAGGATCAC 
CTCGAGAAAG TCAAGGCACT TCGAGGAGAA ATTGCACCTC TCAAAGAGAA TGTAAGCCAC 
GTCAGTGACC TTGCTCGCCA ACTCACCACG TTGGGCATTC AGCTTTCACC ATATAATCTC 
AGCACTCTGG AAGACCTGAA CACCAGATGG AAGCTTCTGC AGGTGGCTGT TGAGGACCGC 
ATCAGGCAGC TGCATGAAGC CCACAGGGAC TTTGGCCCAG CGTCCCAGCA CTTCCTTTCC 
ACTTCTGTCC AGGGTCCCTG GGAGAGAGCC ATCTCACCAA ACAAAGTGCC CTACTATATC 
AACCACGAGA CCCAAACAAC TTGCTGGGAC CATCCTAAAA TGACAGAGCT CTACCAGTCT 
TTAGCTGACC TGAATAATGT CAGGTTCTCG GCTTATAGGA CTGCCATGAA ACTCCGAAGA 
CTGCAGAAGG CCCTTTGCTT GGATCTTTTG AGCCTGTCAG CCGCATGTGA TGCCTTGGAC 
CAGCACAACC TCAAGCAAAA CGACCAGCCC ATGGACATCC TGCAGATCAT TAACTGTTTG 
ACCACTATTT ATGACCGCCT GGAGCAAGAG CACAACAATC TGGTCAACGT CCCTCTCTGC 
GTGGATATGT GTCTCAATTG GCTGCTGAAT GTTTATGATA CGGGACGAAC AGGGAGGATC 
CGGGTCCTGT CTTTTAAAAC TGGCATCATT TCTCTGTGTA AAGCGCATTT GGAAGACAAG 
TACAGATACC TTTTCAAGCA AGTGGCCAGT TCAACAGGAT TTTGTGACCA GCGCAGACTG 
GGCCTCCTTC TACATGACTC TATCCAAATC CCAAGACAGT TGGGTGAAGT TGCATCCTTT 
GGGGGCAGTA ACATTGAGCC AAGTGTCAGG AGCTGCTTCC AATTCGCTAA TAATAAGCCT 
GAGATCGAAG CAGCTCTCTT CCTCGATTGG ATGAGACTAG AACCCCAGTC CATGGTGTGG 
CTGCCCGTCC TGCACAGGGT GGCTGCCGCG GAGACCGCCA AGCATCAGGC CAAGTGTAAC 
ATCTGCAAGG AGTGTCCAAT CATCGGCTTC AGGTACAGAA GTCTAAAGCA CTTTAATTAT 
GACATCTGCC AAAGCTGCTT TTTCTCTGGC CGCGTTGCAA AAGGCCATAA AATGCACTAT 
CCCATGGTGG AATATTGTAC TCCGACTACA TCGGGAGAAG ATGTTCGAGA CTTTGCCAAG 
GTACTAAAAA ACAAATTTCG CACCAAAAGG TATTTTGCGA AGCATCCCCG AATGGGCTAC 
CTGCCAGTGC AGACGGTCTT AGAGGGGGAC AACATGGAAA CTCCTGTTAC TCTGATCAAC 
TTCTGGCCGG TAGACTCTGC GCCTGCCTCG TCCCCTCAGC TTTCACACGA TGATACTCAT 
TCACGCATTG AACATTATGC TAGCAGGCTA GCAGAAATGG AAAACAGCAG TGGATCTTAT 
CTAAATGATA GCATCTCTCC TAATGAGAGC ATAGATGATG AACATTTGTT AATCCAGCAT 
TACTGCCAAA GTTTGAACCA GGACTCCCCC CTGAGCCAGC CTCGTAGTCC TGCCCAGATC 
TTGATTTCCT TAGAGAGTGA GGAAAGAGGG GAGCTAGAGA GAATCCTAGC AGATCTTGAG 
GAAGAAAACA GGAATCTGCA GGCTGAATAT GATCGTCTCA AGGAGCAGCA TGAACATAAA 
GGCCTGTCCC CACTGCCGTC CCCTCCTGAG ATGATGCCCA CCTCGCCACA GAGTCCCCGG 
GATGCTGAGC TCATTGCTGA GGCCAAGCTA CTGCGTCAAC ACAAAGGCCG CCTGGAAGCC 
AGGATGCAAA TCCTGGAAGA CCACAATAAA CAGCTGGAGT CCCAGTTACA CAGGCTCAGG 
CAGCTGCTGG AGCAACCCCA GGCTGAGGCC AAGGTGAATG GCACAACGGT GTCCTCTCCT 
TCTACCTCTC TTCAGAGGTC AGACAGCAGC CAGCCTATGC TGCTCCGGGT GGTTGGCAGT 
CAAACTTCAG AATCCATGGG CGAGGAAGAC CTTCTCAGTC CTCCCCAGGA CTCAAGCACA 
GGGTTAGAGG AAGTGATGGA GCAACTCAAC AACTCCTTCC CTAGTTCCAG AGGACACAAT 
GTAGGAAGCC TTTTCCACTT GGCAGATGAT TTGGGCAGAG CGATGGAGTC CTTAGTTTCA 
GTTATGACAG ATGAAGAAGG GGCAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844127.1
CDS	274..11343
Translation	MEDEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALQVLQKNNVDLVNIGSSDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVRQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYVTSLFQVLPQQVSLEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERTPSSPQPRFKSYAYTQAAYVSTSDPTRSPFPSQRLEAPEDKSFGSPLMETEVNLDSYQTALEEVLSWLLSAEDTLQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGSGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNAWLTKTEERTRKMEKEPLGPDLEDLKRQIQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDVLLKWQRFTEEQCLFSTWLSDKEDALNKIPTSGFKDQSEMLSSLQKLAVLKTDLEKKKQTMDKLCSLNHDLLSTLKNTLVAQKMEAWLDNFAQRWDNLVQKLEKSSTQISQAVTTTQPSLTQTTVMETVTMVTTREQILVKHAQEELPPPPPQKKRQIIVDSEIKKRLDVDITELHSWITRSEAVLQSPEFAVYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTPSEPTAVKSQLKNCKDEVNRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADMQAREKELQTILDTLPTVRYQETMSTILTWIQQSETKLSIPQVTVTEYEIMEQRLEELQALQSSLQEHQNDLNYLSTTVKEMSRKAPSHISQRYQSEFENIEGRWKKLSAQLNERCQKLEEQMAKLRKLQNHIKTLKKWMAEVDVFLKDEWPALGDSEILKKQLKQCRLLVSDIQTIQPSLNSVNEGGQKIKTEAEPEFASRLETELRELNTQWDYICRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVQLLTESVNSVIAQAPPAAQEALRKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLNEVELKLKTTENIPGGAEEISEVLSSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLQLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHYQGKETAQRVLSQIEVAQKKMQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDLELTKRSAVEGMPSNLDSEVAWGKATQKETEKQKVHLKSITELGEALKTVLGKKETLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMETFDQNVDHITKWIIQADALLDESEKKKPQQKEDMLKRLKAELNDIRPKVDSTRDQAANLMANRGDHCRKVIEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVHRQAEGLSEDGAAMAVESTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVMVMTEDMPLEISYVPSTYLTEITHVLQALSEVEQLLNAPDLCAKDFQDLFKQEESLKNIKDNLQQISGRIDVIHNKKAAALQSTTPPEKARLQEALSRLDFQWERVNKMYKDRQGQFDRSVEKWRRFHYDMKIFNQWLTEAEHFLKKTQIPENWEHAKYKWYLKELQDGIGQRQTVVRVLNATGEEIIQQSSKIDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDLNEFVLWLEEAGNISSIPLEPGNEQQLKEKLEEVKLLAEELPLRQGILKQLNETGGTVLVSAPISPEEQDKLENKLKQTNLQWIKVSRSLPEKQGEIEAHIKDLGQLEEQLNHLLLWLSPIRSQLEIYNQPNQTGPFDIKETEVAVQAKQPDVEGILSKGQNLYKEKPATEPVKRKLEDLSSEWKAVTHLLQELRAKWPGPVPGLTATGAPPSQTVALVTQPVVAKETAVSKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVLKAQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVVGQARAKLETWKEGPYTMDAIQRKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRVSERETALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKILRSLEGSDDAVLLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDIHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEEKAQNVTRLLRKQAEEVNTEWEKLNLHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVSHVSDLARQLTTLGIQLSPYNLSTLEDLNTRWKLLQVAVEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSSGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKEQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDSSTGLEEVMEQLNNSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_024988359.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophin (DMD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988359.1

ORF Insert Sequence:

ATGGAAGATG AAAGAGAAGA TGTTCAAAAG AAAACATTCA CAAAATGGAT AAATGCACAA 
TTTTCTAAGT TCGGGAAGCA GCACATAGAA AACCTCTTCA GTGACCTACA GGATGGGAGA 
CGCCTCCTAG ACCTTCTTGA AGGCCTGACA GGGCAAAAAC TGCCAAAAGA AAAAGGATCC 
ACAAGAGTTC ATGCCCTGAA CAATGTCAAC AAGGCACTGC AGGTCTTGCA GAAAAATAAT 
GTTGATTTAG TGAATATCGG AAGCTCTGAC ATAGTAGATG GAAATCATAA ACTGACTCTT 
GGCTTGATTT GGAATATAAT CCTCCATTGG CAGGTCAAAA ATGTAATGAA AAATATCATG 
GCTGGATTGC AACAAACCAA CAGTGAGAAG ATACTCCTGA GCTGGGTCCG ACAATCAACT 
CGTAATTATC CACAGGTTAA TGTCATCAAC TTCACCACCA GCTGGTCTGA CGGTTTGGCT 
TTGAATGCGC TCATCCACAG TCATAGGCCA GACCTGTTTG ATTGGAATAG TGTGGTTCGC 
CAGCAGTCAG CTACACAACG ACTGGAACAT GCATTCAACA TTGCCAAATA TCAATTAGGC 
ATAGAGAAAC TACTGGATCC TGAAGATGTT GCCACCACTT ATCCAGATAA GAAGTCCATC 
TTAATGTACG TCACATCTCT CTTCCAAGTT TTGCCTCAAC AAGTGAGCCT TGAAGCCATC 
CAGGAGGTGG AAATGTTGCC AAGGCCATCA AAAGTTACAA GAGAAGAACA TTTTCAACTA 
CATCATCAAA TGCACTACTC TCAACAGATC ACTGTCAGTC TAGCACAGGG CTATGAACGA 
ACTCCTTCCT CTCCTCAGCC TCGCTTCAAG AGCTATGCCT ACACGCAGGC TGCTTATGTC 
TCCACCTCTG ACCCCACACG GAGCCCATTT CCTTCACAGC GTTTGGAAGC TCCTGAAGAC 
AAGTCATTTG GCAGTCCATT GATGGAGACT GAAGTAAACC TGGACAGTTA CCAGACAGCT 
TTAGAAGAAG TACTCTCATG GCTCCTTTCA GCCGAAGACA CATTGCAAGC ACAAGGAGAG 
ATTTCTAATG ATGTAGAAGA AGTGAAAGAA CAGTTTCATA CCCACGAGGG ATACATGATG 
GATTTGACAT CCCATCAGGG ACGGGTTGGC AATGTTCTCC AATTGGGGAG TCAGCTGATT 
GGGTCAGGGA AATTATCAGA AGATGAAGAA ACTGAAGTGC AAGAACAAAT GAATCTTCTA 
AATTCAAGAT GGGAATGCCT CAGGGTAGCT AGCATGGAAA AACAAAGCAA TTTACATAAA 
GTTCTCATGG ATCTCCAGAA TCAGCAGCTG AAAGAGTTAA ATGCCTGGTT AACAAAAACA 
GAAGAGAGAA CAAGGAAAAT GGAAAAAGAG CCCCTTGGAC CTGATCTTGA GGACCTCAAA 
CGCCAAATAC AGCAACATAA GGTGCTTCAA GAAGACCTAG AACAGGAACA AGTCAGAGTC 
AATTCTCTCA CGCACATGGT GGTGGTTGTA GATGAGTCTA GTGGTGATCA TGCAACTGCT 
GCTTTGGAAG AACAACTTAA GGTACTGGGA GATCGGTGGG CAAACATCTG TAGGTGGACT 
GAAGATCGCT GGGTTCTTTT ACAGGATGTT CTTCTAAAAT GGCAACGTTT TACTGAAGAG 
CAGTGCCTTT TTAGTACATG GCTTTCAGAT AAAGAAGATG CATTGAACAA GATTCCCACA 
AGTGGCTTTA AGGATCAAAG TGAAATGTTA TCAAGTCTTC AAAAACTGGC CGTTTTAAAA 
ACTGATCTAG AAAAGAAAAA GCAAACCATG GACAAACTCT GCTCACTCAA CCATGATCTC 
CTTTCAACAC TGAAAAATAC ATTGGTTGCC CAAAAGATGG AAGCATGGTT GGACAACTTT 
GCCCAGCGCT GGGATAATTT AGTCCAAAAA CTTGAAAAGA GTTCAACACA GATTTCACAG 
GCTGTCACCA CCACTCAGCC ATCACTAACA CAGACAACTG TAATGGAAAC GGTAACTATG 
GTGACCACGA GGGAGCAAAT CCTGGTAAAG CATGCCCAAG AGGAACTCCC TCCACCACCT 
CCACAAAAGA AGAGGCAGAT TATTGTGGAT TCTGAAATTA AGAAAAGGTT GGATGTCGAT 
ATAACTGAAC TTCACAGTTG GATTACTCGT TCAGAAGCCG TGTTACAGAG TCCTGAATTT 
GCAGTCTATC GGAAGGAAGG CAACTTCTCA GACCTTAAAG AAAAAGTCAA TGCCATAGAG 
CGAGAAAAAG CCGAGAAGTT CAGAAAACTG CAAGATGCCA GCAGATCAGC TCAGGCCCTG 
GTGGAACAGA TGGTGAATGA GGGTGTTAAT GCTGACAGTA TCAAACAAGC CTCAGAACAA 
CTGAACAGCC GGTGGATCGA GTTCTGCCAA TTGCTAAGTG AGAGACTTAA CTGGCTGGAG 
TATCAGAACA ACATCATCAC TTTCTATAAT CAGCTACAAC AATTGGAGCA GATGACAACT 
ACTGCAGAAA ACTGGTTGAA AACTCAACCC ACTACCCCAT CAGAGCCAAC AGCAGTTAAA 
AGCCAGTTAA AAAATTGTAA GGATGAAGTC AACCGGCTGT CAGCTCTTCA GCCTCAAATC 
GAGAGATTAA AAATTCAAAG CATAGCCCTG AAAGAGAAAG GACAAGGGCC AATGTTCCTG 
GATGCCGACT TTGTGGCCTT TACAAATCAT TTTAACCAAG TCTTTGCTGA TATGCAGGCC 
AGAGAGAAAG AGTTACAGAC AATTTTGGAC ACTTTACCCA CCGTGCGGTA TCAGGAGACC 
ATGAGTACCA TCCTCACGTG GATCCAACAG TCAGAAACCA AACTCTCGAT ACCTCAGGTT 
ACTGTCACTG AATACGAGAT CATGGAGCAG AGACTTGAGG AGTTACAGGC TTTACAAAGC 
TCTCTGCAAG AGCACCAAAA TGACCTAAAC TATCTCAGCA CCACTGTGAA AGAGATGTCA 
AGGAAAGCAC CCTCTCATAT AAGCCAGAGA TATCAGTCAG AATTTGAAAA CATTGAGGGT 
CGGTGGAAGA AACTCTCTGC CCAGCTGAAT GAACGTTGCC AAAAGCTAGA GGAGCAAATG 
GCCAAACTCC GAAAACTTCA GAATCACATA AAAACACTGA AGAAGTGGAT GGCTGAAGTT 
GATGTTTTCT TGAAAGATGA ATGGCCTGCC CTTGGAGATT CAGAAATTCT TAAAAAACAG 
CTGAAGCAGT GCAGGCTTTT AGTCAGTGAT ATTCAGACAA TCCAGCCCAG TCTAAACAGT 
GTCAATGAAG GTGGACAGAA AATAAAAACT GAAGCGGAGC CAGAGTTTGC TTCTAGACTG 
GAGACAGAGC TCAGAGAACT TAACACTCAA TGGGATTACA TATGTCGACA GGTCTATGCC 
AGAAAGGAGG CCTTAAAGGG AGGCTTGGAT AAAACTGTAA GCCTTCAGAA AGATTTGTCA 
GAGATGCATG AATGGATGAC ACAAGCCGAA GAAGAATACC TAGAGAGAGA TTTTGAATAT 
AAAACCCCAG ATGAATTACA GACAGCAGTT GAAGAGATGA AGAGAGCTAA AGAAGAGGCC 
CAGCAAAAAG AAGCAAAAGT GCAGCTCCTC ACTGAGTCCG TGAACAGTGT CATAGCTCAA 
GCTCCACCTG CAGCACAAGA GGCCTTAAGA AAGGAACTGG ACACTCTGAC CACCAACTAC 
CAGTGGCTCT GCACCAGGCT GAATGGAAAA TGCAAGACGT TAGAAGAGGT TTGGGCATGT 
TGGCATGAGT TATTGTCATA CTTGGAAAAG GCAAATAAGT GGCTAAATGA AGTAGAATTG 
AAACTTAAGA CTACTGAAAA TATTCCTGGT GGAGCTGAGG AAATCTCTGA GGTGCTCAGT 
TCACTTGAAA ATTTGATGCA ACATTCAGAA GATAACCCGA ATCAGATTCG CATATTGGCA 
CAGACCCTAA CAGATGGTGG AGTCATGGAT GAACTGATCA ATGAAGAACT CGAGACATTT 
AATTCTCGTT GGAGAGAACT ACATGAAGAG GCTGTGAGGA GGCAAAAGTT GCTTGAACAG 
AGTATCCAGT CTGCCCAGGA GATTGAAAAA TCCTTACAGT TAATTCAGGA ATCCCTCTCA 
TCAATTGACA AGCAGTTGGC AGCTTATATC GCTGACAAAG TGGACGCCGC TCAGATGCCT 
CAGGAAGCCC AGAAAATCCA ATCTGATTTG ACAAGTCATG AGATCAGTTT AGAAGAAATG 
AAGAAACATT ACCAAGGAAA GGAAACTGCT CAACGGGTAC TATCCCAAAT TGAAGTGGCA 
CAGAAAAAAA TGCAAGATGT TTCCATGAAA TTTCGATTGT TCCAGAAGCC AGCCAATTTT 
GAGCAGCGTC TACAGGAAAG TAAGATGATT TTAGATGAAG TGAAGATGCA CTTGCCCGCA 
CTGGAAACAA AGAGTGTTGA ACAGGAAGTA GTACAGTCAC AGTTAAATCA TTGTGTGAAC 
TTGTATAAAA GTCTGAGTGA AGTGAAGTCT GAAGTGGAAA TGGTGATTAA AACTGGACGT 
CAAATTGTAC AGAAGAAGCA GACGGAAAAC CCTAAAGAGC TTGATGAAAG AGTAACGGCT 
TTGAAATTGC ATTATAATGA GCTGGGAGCA AAGGTAACAG AAAGAAAGCA ACAGTTGGAG 
AAATGCTTGA AATTGTCCCG TAAGATGCGA AAGGAAATGA ATGCCTTGAC AGAATGGCTG 
GCAGCTACAG ATTTGGAACT GACAAAGAGA TCAGCAGTTG AAGGAATGCC TAGTAATTTG 
GATTCTGAAG TTGCCTGGGG AAAGGCTACT CAGAAAGAGA CTGAGAAACA GAAGGTTCAC 
CTAAAGAGCA TTACAGAGCT AGGAGAGGCC TTGAAAACGG TTTTGGGCAA GAAGGAGACC 
TTGGTCGAAG ATAAACTGAG TCTTTTGAAT AGTAACTGGA TAGCCGTCAC TTCTCGAGCA 
GAAGAGTGGC TAAACCTTTT GTTGGAATAC CAGAAACACA TGGAAACCTT TGACCAGAAT 
GTGGATCATA TCACAAAGTG GATCATTCAA GCTGATGCAC TTCTGGATGA ATCTGAGAAA 
AAGAAACCAC AGCAAAAAGA AGACATGCTA AAGCGCTTAA AGGCTGAACT GAATGACATA 
CGCCCAAAGG TGGACTCCAC GCGTGACCAA GCAGCAAACT TGATGGCAAA CCGCGGCGAC 
CACTGCAGGA AAGTAATAGA GCCCAAAATC TCAGAGCTCA ACCATCGATT TGCAGCCATT 
TCTCACAGAA TTAAGACTGG AAAGGCCTCC ATTCCTTTGA AGGAATTGGA GCAGTTTAAC 
TCAGATATAC AAAAATTGCT TGAACCACTG GAGGCTGAAA TTCAGCAGGG GGTGAATCTG 
AAAGAGGAAG ACTTCAATAA AGATATGAGT GAAGACAATG AGGGTACTGT AAAAGAATTG 
TTGCAAAGAG GAGACAACTT ACAACAAAGA ATCACAGATG AGAGAAAGCG AGAGGAAATA 
AAGATAAAAC AGCAGCTGTT ACAGACAAAA CATAATGCTC TCAAGGATTT GAGGTCTCAA 
AGAAGAAAAA AGGCTCTAGA AATTTCTCAC CAGTGGTATC AGTACAAGAG GCAGGCTGAT 
GATCTCCTGA AATGCTTGGA TGACATTGAA AAAAAATTAG CCAGCCTACC TGAACCCAGA 
GATGAAAGGA AAATAAAGGA AATTGATCGT GAGTTGCAGA AGAAGAAAGA GGAGCTGAAT 
GCAGTGCATA GGCAAGCTGA GGGCTTGTCT GAGGATGGGG CAGCAATGGC AGTGGAGTCA 
ACTCAGATCC AGCTCAGCAA GCGCTGGCGG GAAATTGAGA GCAAATTTGC TCAGTTTCGA 
AGACTCAACT TTGCACAAAT TCACACTGTC CATGAAGAAT CAGTGATGGT GATGACCGAA 
GACATGCCTT TGGAAATTTC TTATGTGCCT TCTACTTACC TGACTGAGAT CACTCACGTC 
TTACAAGCCC TTTCAGAAGT GGAACAACTT CTCAATGCTC CCGACCTCTG TGCTAAAGAT 
TTTCAAGATC TCTTCAAACA AGAGGAATCT CTGAAGAATA TAAAAGACAA CCTGCAACAA 
ATCTCAGGTC GGATTGATGT TATTCACAAT AAGAAGGCGG CAGCATTGCA AAGCACCACA 
CCCCCAGAAA AGGCAAGGTT ACAGGAAGCT CTCTCCAGGC TCGACTTCCA ATGGGAAAGA 
GTGAACAAAA TGTACAAGGA CCGACAAGGG CAATTTGACA GATCTGTTGA AAAATGGCGA 
CGGTTTCATT ATGATATGAA GATATTTAAT CAATGGCTAA CAGAAGCTGA ACACTTTCTC 
AAAAAGACAC AAATTCCTGA GAACTGGGAA CATGCCAAAT ATAAATGGTA TCTTAAGGAA 
CTCCAGGATG GCATCGGGCA GCGGCAAACT GTTGTCAGAG TATTGAATGC AACTGGGGAA 
GAAATAATTC AGCAGTCCTC AAAAATAGAT GCCAGTATTC TACAAGAAAA ATTGGGAAGC 
CTGAATCTGC GGTGGCAGGA GGTCTGCAAA CAGCTGGCCG AGAGAAAAAA GAGGTTAGAA 
GAACAAAAGA ATATCCTGTC AGAATTTCAA AGAGATTTGA ATGAATTTGT TTTATGGTTG 
GAAGAAGCAG GTAACATTTC TAGTATTCCT CTTGAACCTG GAAATGAGCA GCAACTAAAA 
GAAAAGCTTG AGGAAGTCAA GTTACTGGCC GAAGAGTTGC CCCTGCGTCA GGGAATTCTA 
AAACAATTAA ATGAAACAGG AGGAACAGTG CTTGTAAGTG CTCCCATAAG CCCAGAAGAG 
CAAGATAAAC TTGAAAATAA GCTCAAGCAG ACAAATCTTC AGTGGATAAA GGTTTCTAGG 
AGTTTACCTG AGAAACAGGG AGAAATTGAG GCTCACATAA AAGACCTTGG ACAGCTTGAA 
GAGCAATTAA ATCATCTGCT TCTGTGGCTG TCTCCTATTA GGAGTCAGTT GGAAATTTAC 
AACCAACCAA ATCAGACAGG ACCATTTGAC ATTAAGGAAA CTGAAGTAGC AGTTCAAGCT 
AAACAGCCGG ATGTGGAAGG GATTTTGTCT AAAGGGCAGA ATTTGTACAA GGAAAAACCA 
GCCACTGAGC CAGTGAAGAG GAAGTTAGAA GATCTGAGCT CGGAGTGGAA GGCGGTAACC 
CACTTACTTC AAGAGCTGAG GGCAAAGTGG CCTGGCCCAG TCCCTGGACT GACAGCAACT 
GGAGCACCTC CTAGTCAGAC GGTTGCTCTG GTGACACAAC CTGTGGTTGC CAAGGAAACC 
GCTGTCTCCA AACTAGAAAT GCCATCTTCC TTGCTGTTGG AGGTCCCTGC ACTGGCAGAT 
TTCAACCGGG CTTGGACAGA ACTTACCGAC TGGCTGTCTC TGCTTGATCG AGTTCTGAAA 
GCACAGAGGG TGATGGTGGG TGATCTTGAA GACATCAACG AGATGATCAT CAAGCAGAAG 
GCAACACTGC AGGACTTGGA GCAGAGGCGC CCCCAGTTGG AAGAACTCAT TACTGCTGCC 
CAGAATTTGA AAAACAAGAC CAGCAATCAA GAGGCCAGGA CCATCATTAC TGATCGAATT 
GAAAGAATTC AGAGTCAGTG GGATGAAGTA CAGGAACACC TTCAGAACCG GAGGCAGCAG 
TTAAATGAAA TGTTAAAGGA TTCAACACAA TGGCTGGAAG CTAAGGAAGA AGCTGAGCAG 
GTGGTGGGGC AGGCCAGAGC CAAGCTTGAG ACATGGAAGG AGGGTCCCTA CACAATGGAT 
GCAATCCAAA GGAAGATCAC AGAAACCAAG CAGTTGGCCA AAGACCTCCG CCAGTGGCAG 
ATAAATGTAG ATGTGGCAAA TGACTTGGCA CTGAAACTTC TCCGGGATTA TTCTGCAGAT 
GATACCCGAA AGGTACACAT GATAACAGAG AACATCAATG CCTCTTGGGC AAGCATTCAT 
AAAAGAGTGA GTGAGCGAGA GACTGCTCTG GAAGAAACTC ATAGATTACT GCAACAGTTC 
CCCCTGGACT TGGAGAAGTT TCTTGCCTGG CTTACAGAAG CTGAAACAAC TGCCAACGTC 
CTGCAGGATG CCACCCATAA GGAAAGGCTT CTAGAAGACT CCAAGGGAGT AAGAGAGCTG 
ATGAAACAAT GGCAGGACCT CCAAGGAGAA ATTGAAGCTC ACACAGATAT CTATCACAAC 
CTAGATGAAA ATGGCCAAAA AATCCTGAGA TCCCTGGAAG GTTCTGATGA CGCGGTCCTA 
TTACAAAGAC GTTTGGATAA TATGAATTTC AAGTGGAGTG AGCTGCGGAA AAAGTCTCTC 
AATATCAGGT CCCATTTGGA AGCCAGTTCT GACCAGTGGA AGCGTCTGCA CCTTTCTCTT 
CAGGAACTTC TGGTGTGGCT ACAGCTGAAA GACGATGAGT TGAGCCGTCA GGCACCTATT 
GGAGGCGATT TCCCAGCAGT TCAGAAGCAG AATGATATAC ACAGGGCCTT CAAGAGGGAA 
CTGAAAACGA AAGAACCTGT AATCATGAGT ACTCTTGAGA CTGTACGAAT ATTTCTGACA 
GAGCAGCCTT TGGAAGGACT AGAGAAACTC TACCAGGAGC CCAGAGAGCT GCCGCCTGAG 
GAGAAAGCCC AGAATGTCAC ACGACTCCTA CGAAAGCAGG CTGAGGAGGT CAACACAGAG 
TGGGAAAAAT TGAACCTACA CTCGGCTGAC TGGCAGAGAA AAATAGACGA GGCCCTCGAA 
AGACTCCAAG AACTGCAGGA GGCCACGGAC GAGCTGGACC TCAAACTGCG CCAAGCTGAG 
GTGATCAAGG GATCATGGCA GCCCGTGGGC GATCTTCTCA TTGACTCCCT CCAGGATCAC 
CTCGAGAAAG TCAAGGCACT TCGAGGAGAA ATTGCACCTC TCAAAGAGAA TGTAAGCCAC 
GTCAGTGACC TTGCTCGCCA ACTCACCACG TTGGGCATTC AGCTTTCACC ATATAATCTC 
AGCACTCTGG AAGACCTGAA CACCAGATGG AAGCTTCTGC AGGTGGCTGT TGAGGACCGC 
ATCAGGCAGC TGCATGAAGC CCACAGGGAC TTTGGCCCAG CGTCCCAGCA CTTCCTTTCC 
ACTTCTGTCC AGGGTCCCTG GGAGAGAGCC ATCTCACCAA ACAAAGTGCC CTACTATATC 
AACCACGAGA CCCAAACAAC TTGCTGGGAC CATCCTAAAA TGACAGAGCT CTACCAGTCT 
TTAGCTGACC TGAATAATGT CAGGTTCTCG GCTTATAGGA CTGCCATGAA ACTCCGAAGA 
CTGCAGAAGG CCCTTTGCTT GGATCTTTTG AGCCTGTCAG CCGCATGTGA TGCCTTGGAC 
CAGCACAACC TCAAGCAAAA CGACCAGCCC ATGGACATCC TGCAGATCAT TAACTGTTTG 
ACCACTATTT ATGACCGCCT GGAGCAAGAG CACAACAATC TGGTCAACGT CCCTCTCTGC 
GTGGATATGT GTCTCAATTG GCTGCTGAAT GTTTATGATA CGGGACGAAC AGGGAGGATC 
CGGGTCCTGT CTTTTAAAAC TGGCATCATT TCTCTGTGTA AAGCGCATTT GGAAGACAAG 
TACAGATACC TTTTCAAGCA AGTGGCCAGT TCAACAGGAT TTTGTGACCA GCGCAGACTG 
GGCCTCCTTC TACATGACTC TATCCAAATC CCAAGACAGT TGGGTGAAGT TGCATCCTTT 
GGGGGCAGTA ACATTGAGCC AAGTGTCAGG AGCTGCTTCC AATTCGCTAA TAATAAGCCT 
GAGATCGAAG CAGCTCTCTT CCTCGATTGG ATGAGACTAG AACCCCAGTC CATGGTGTGG 
CTGCCCGTCC TGCACAGGGT GGCTGCCGCG GAGACCGCCA AGCATCAGGC CAAGTGTAAC 
ATCTGCAAGG AGTGTCCAAT CATCGGCTTC AGGTACAGAA GTCTAAAGCA CTTTAATTAT 
GACATCTGCC AAAGCTGCTT TTTCTCTGGC CGCGTTGCAA AAGGCCATAA AATGCACTAT 
CCCATGGTGG AATATTGTAC TCCGACTACA TCGGGAGAAG ATGTTCGAGA CTTTGCCAAG 
GTACTAAAAA ACAAATTTCG CACCAAAAGG TATTTTGCGA AGCATCCCCG AATGGGCTAC 
CTGCCAGTGC AGACGGTCTT AGAGGGGGAC AACATGGAAA CTCCTGTTAC TCTGATCAAC 
TTCTGGCCGG TAGACTCTGC GCCTGCCTCG TCCCCTCAGC TTTCACACGA TGATACTCAT 
TCACGCATTG AACATTATGC TAGCAGGCTA GCAGAAATGG AAAACAGCAG TGGATCTTAT 
CTAAATGATA GCATCTCTCC TAATGAGAGC ATAGATGATG AACATTTGTT AATCCAGCAT 
TACTGCCAAA GTTTGAACCA GGACTCCCCC CTGAGCCAGC CTCGTAGTCC TGCCCAGATC 
TTGATTTCCT TAGAGAGTGA GGAAAGAGGG GAGCTAGAGA GAATCCTAGC AGATCTTGAG 
GAAGAAAACA GGAATCTGCA GGCTGAATAT GATCGTCTCA AGGAGCAGCA TGAACATAAA 
GGCCTGTCCC CACTGCCGTC CCCTCCTGAG ATGATGCCCA CCTCGCCACA GAGTCCCCGG 
GATGCTGAGC TCATTGCTGA GGCCAAGCTA CTGCGTCAAC ACAAAGGCCG CCTGGAAGCC 
AGGATGCAAA TCCTGGAAGA CCACAATAAA CAGCTGGAGT CCCAGTTACA CAGGCTCAGG 
CAGCTGCTGG AGCAACCCCA GGCTGAGGCC AAGGTGAATG GCACAACGGT GTCCTCTCCT 
TCTACCTCTC TTCAGAGGTC AGACAGCAGC CAGCCTATGC TGCTCCGGGT GGTTGGCAGT 
CAAACTTCAG AATCCATGGG CGAGGAAGAC CTTCTCAGTC CTCCCCAGGA CTCAAGCACA 
GGGTTAGAGG AAGTGATGGA GCAACTCAAC AACTCCTTCC CTAGTTCCAG AGGACACAAT 
GTAGGAAGCC TTTTCCACTT GGCAGATGAT TTGGGCAGAG CGATGGAGTC CTTAGTTTCA 
GTTATGACAG ATGAAGAAGG GGCAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_024988360.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844128.1 Bos taurus dystrophin (DMD), transcript variant X3, mRNA.

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CloneID	OBa238539
Clone ID Related Accession (Same CDS sequence)	XM_024988360.1
Accession Version	XM_024988360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAATTTTCT AAGTTCGGGA AGCAGCACAT AGAAAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTC TTGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCAGGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAATA TCGGAAGCTC TGACATAGTA 
GATGGAAATC ATAAACTGAC TCTTGGCTTG ATTTGGAATA TAATCCTCCA TTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA GAAGATACTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT CAACTTCACC 
ACCAGCTGGT CTGACGGTTT GGCTTTGAAT GCGCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TCGCCAGCAG TCAGCTACAC AACGACTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTACTGG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TACGTCACAT CTCTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCCTTGAAGC CATCCAGGAG GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACAAGAGAAG AACATTTTCA ACTACATCAT CAAATGCACT ACTCTCAACA GATCACTGTC 
AGTCTAGCAC AGGGCTATGA ACGAACTCCT TCCTCTCCTC AGCCTCGCTT CAAGAGCTAT 
GCCTACACGC AGGCTGCTTA TGTCTCCACC TCTGACCCCA CACGGAGCCC ATTTCCTTCA 
CAGCGTTTGG AAGCTCCTGA AGACAAGTCA TTTGGCAGTC CATTGATGGA GACTGAAGTA 
AACCTGGACA GTTACCAGAC AGCTTTAGAA GAAGTACTCT CATGGCTCCT TTCAGCCGAA 
GACACATTGC AAGCACAAGG AGAGATTTCT AATGATGTAG AAGAAGTGAA AGAACAGTTT 
CATACCCACG AGGGATACAT GATGGATTTG ACATCCCATC AGGGACGGGT TGGCAATGTT 
CTCCAATTGG GGAGTCAGCT GATTGGGTCA GGGAAATTAT CAGAAGATGA AGAAACTGAA 
GTGCAAGAAC AAATGAATCT TCTAAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTC ATGGATCTCC AGAATCAGCA GCTGAAAGAG 
TTAAATGCCT GGTTAACAAA AACAGAAGAG AGAACAAGGA AAATGGAAAA AGAGCCCCTT 
GGACCTGATC TTGAGGACCT CAAACGCCAA ATACAGCAAC ATAAGGTGCT TCAAGAAGAC 
CTAGAACAGG AACAAGTCAG AGTCAATTCT CTCACGCACA TGGTGGTGGT TGTAGATGAG 
TCTAGTGGTG ATCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGG 
TGGGCAAACA TCTGTAGGTG GACTGAAGAT CGCTGGGTTC TTTTACAGGA TGTTCTTCTA 
AAATGGCAAC GTTTTACTGA AGAGCAGTGC CTTTTTAGTA CATGGCTTTC AGATAAAGAA 
GATGCATTGA ACAAGATTCC CACAAGTGGC TTTAAGGATC AAAGTGAAAT GTTATCAAGT 
CTTCAAAAAC TGGCCGTTTT AAAAACTGAT CTAGAAAAGA AAAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCATGA TCTCCTTTCA ACACTGAAAA ATACATTGGT TGCCCAAAAG 
ATGGAAGCAT GGTTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA AAAACTTGAA 
AAGAGTTCAA CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACGGTAAC TATGGTGACC ACGAGGGAGC AAATCCTGGT AAAGCATGCC 
CAAGAGGAAC TCCCTCCACC ACCTCCACAA AAGAAGAGGC AGATTATTGT GGATTCTGAA 
ATTAAGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCCGTGTTAC AGAGTCCTGA ATTTGCAGTC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGTATCAAAC AAGCCTCAGA ACAACTGAAC AGCCGGTGGA TCGAGTTCTG CCAATTGCTA 
AGTGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCA GAAAACTGGT TGAAAACTCA ACCCACTACC 
CCATCAGAGC CAACAGCAGT TAAAAGCCAG TTAAAAAATT GTAAGGATGA AGTCAACCGG 
CTGTCAGCTC TTCAGCCTCA AATCGAGAGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCC GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATATGCA GGCCAGAGAG AAAGAGTTAC AGACAATTTT GGACACTTTA 
CCCACCGTGC GGTATCAGGA GACCATGAGT ACCATCCTCA CGTGGATCCA ACAGTCAGAA 
ACCAAACTCT CGATACCTCA GGTTACTGTC ACTGAATACG AGATCATGGA GCAGAGACTT 
GAGGAGTTAC AGGCTTTACA AAGCTCTCTG CAAGAGCACC AAAATGACCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAGGAAA GCACCCTCTC ATATAAGCCA GAGATATCAG 
TCAGAATTTG AAAACATTGA GGGTCGGTGG AAGAAACTCT CTGCCCAGCT GAATGAACGT 
TGCCAAAAGC TAGAGGAGCA AATGGCCAAA CTCCGAAAAC TTCAGAATCA CATAAAAACA 
CTGAAGAAGT GGATGGCTGA AGTTGATGTT TTCTTGAAAG ATGAATGGCC TGCCCTTGGA 
GATTCAGAAA TTCTTAAAAA ACAGCTGAAG CAGTGCAGGC TTTTAGTCAG TGATATTCAG 
ACAATCCAGC CCAGTCTAAA CAGTGTCAAT GAAGGTGGAC AGAAAATAAA AACTGAAGCG 
GAGCCAGAGT TTGCTTCTAG ACTGGAGACA GAGCTCAGAG AACTTAACAC TCAATGGGAT 
TACATATGTC GACAGGTCTA TGCCAGAAAG GAGGCCTTAA AGGGAGGCTT GGATAAAACT 
GTAAGCCTTC AGAAAGATTT GTCAGAGATG CATGAATGGA TGACACAAGC CGAAGAAGAA 
TACCTAGAGA GAGATTTTGA ATATAAAACC CCAGATGAAT TACAGACAGC AGTTGAAGAG 
ATGAAGAGAG CTAAAGAAGA GGCCCAGCAA AAAGAAGCAA AAGTGCAGCT CCTCACTGAG 
TCCGTGAACA GTGTCATAGC TCAAGCTCCA CCTGCAGCAC AAGAGGCCTT AAGAAAGGAA 
CTGGACACTC TGACCACCAA CTACCAGTGG CTCTGCACCA GGCTGAATGG AAAATGCAAG 
ACGTTAGAAG AGGTTTGGGC ATGTTGGCAT GAGTTATTGT CATACTTGGA AAAGGCAAAT 
AAGTGGCTAA ATGAAGTAGA ATTGAAACTT AAGACTACTG AAAATATTCC TGGTGGAGCT 
GAGGAAATCT CTGAGGTGCT CAGTTCACTT GAAAATTTGA TGCAACATTC AGAAGATAAC 
CCGAATCAGA TTCGCATATT GGCACAGACC CTAACAGATG GTGGAGTCAT GGATGAACTG 
ATCAATGAAG AACTCGAGAC ATTTAATTCT CGTTGGAGAG AACTACATGA AGAGGCTGTG 
AGGAGGCAAA AGTTGCTTGA ACAGAGTATC CAGTCTGCCC AGGAGATTGA AAAATCCTTA 
CAGTTAATTC AGGAATCCCT CTCATCAATT GACAAGCAGT TGGCAGCTTA TATCGCTGAC 
AAAGTGGACG CCGCTCAGAT GCCTCAGGAA GCCCAGAAAA TCCAATCTGA TTTGACAAGT 
CATGAGATCA GTTTAGAAGA AATGAAGAAA CATTACCAAG GAAAGGAAAC TGCTCAACGG 
GTACTATCCC AAATTGAAGT GGCACAGAAA AAAATGCAAG ATGTTTCCAT GAAATTTCGA 
TTGTTCCAGA AGCCAGCCAA TTTTGAGCAG CGTCTACAGG AAAGTAAGAT GATTTTAGAT 
GAAGTGAAGA TGCACTTGCC CGCACTGGAA ACAAAGAGTG TTGAACAGGA AGTAGTACAG 
TCACAGTTAA ATCATTGTGT GAACTTGTAT AAAAGTCTGA GTGAAGTGAA GTCTGAAGTG 
GAAATGGTGA TTAAAACTGG ACGTCAAATT GTACAGAAGA AGCAGACGGA AAACCCTAAA 
GAGCTTGATG AAAGAGTAAC GGCTTTGAAA TTGCATTATA ATGAGCTGGG AGCAAAGGTA 
ACAGAAAGAA AGCAACAGTT GGAGAAATGC TTGAAATTGT CCCGTAAGAT GCGAAAGGAA 
ATGAATGCCT TGACAGAATG GCTGGCAGCT ACAGATTTGG AACTGACAAA GAGATCAGCA 
GTTGAAGGAA TGCCTAGTAA TTTGGATTCT GAAGTTGCCT GGGGAAAGGC TACTCAGAAA 
GAGACTGAGA AACAGAAGGT TCACCTAAAG AGCATTACAG AGCTAGGAGA GGCCTTGAAA 
ACGGTTTTGG GCAAGAAGGA GACCTTGGTC GAAGATAAAC TGAGTCTTTT GAATAGTAAC 
TGGATAGCCG TCACTTCTCG AGCAGAAGAG TGGCTAAACC TTTTGTTGGA ATACCAGAAA 
CACATGGAAA CCTTTGACCA GAATGTGGAT CATATCACAA AGTGGATCAT TCAAGCTGAT 
GCACTTCTGG ATGAATCTGA GAAAAAGAAA CCACAGCAAA AAGAAGACAT GCTAAAGCGC 
TTAAAGGCTG AACTGAATGA CATACGCCCA AAGGTGGACT CCACGCGTGA CCAAGCAGCA 
AACTTGATGG CAAACCGCGG CGACCACTGC AGGAAAGTAA TAGAGCCCAA AATCTCAGAG 
CTCAACCATC GATTTGCAGC CATTTCTCAC AGAATTAAGA CTGGAAAGGC CTCCATTCCT 
TTGAAGGAAT TGGAGCAGTT TAACTCAGAT ATACAAAAAT TGCTTGAACC ACTGGAGGCT 
GAAATTCAGC AGGGGGTGAA TCTGAAAGAG GAAGACTTCA ATAAAGATAT GAGTGAAGAC 
AATGAGGGTA CTGTAAAAGA ATTGTTGCAA AGAGGAGACA ACTTACAACA AAGAATCACA 
GATGAGAGAA AGCGAGAGGA AATAAAGATA AAACAGCAGC TGTTACAGAC AAAACATAAT 
GCTCTCAAGG ATTTGAGGTC TCAAAGAAGA AAAAAGGCTC TAGAAATTTC TCACCAGTGG 
TATCAGTACA AGAGGCAGGC TGATGATCTC CTGAAATGCT TGGATGACAT TGAAAAAAAA 
TTAGCCAGCC TACCTGAACC CAGAGATGAA AGGAAAATAA AGGAAATTGA TCGTGAGTTG 
CAGAAGAAGA AAGAGGAGCT GAATGCAGTG CATAGGCAAG CTGAGGGCTT GTCTGAGGAT 
GGGGCAGCAA TGGCAGTGGA GTCAACTCAG ATCCAGCTCA GCAAGCGCTG GCGGGAAATT 
GAGAGCAAAT TTGCTCAGTT TCGAAGACTC AACTTTGCAC AAATTCACAC TGTCCATGAA 
GAATCAGTGA TGGTGATGAC CGAAGACATG CCTTTGGAAA TTTCTTATGT GCCTTCTACT 
TACCTGACTG AGATCACTCA CGTCTTACAA GCCCTTTCAG AAGTGGAACA ACTTCTCAAT 
GCTCCCGACC TCTGTGCTAA AGATTTTCAA GATCTCTTCA AACAAGAGGA ATCTCTGAAG 
AATATAAAAG ACAACCTGCA ACAAATCTCA GGTCGGATTG ATGTTATTCA CAATAAGAAG 
GCGGCAGCAT TGCAAAGCAC CACACCCCCA GAAAAGGCAA GGTTACAGGA AGCTCTCTCC 
AGGCTCGACT TCCAATGGGA AAGAGTGAAC AAAATGTACA AGGACCGACA AGGGCAATTT 
GACAGATCTG TTGAAAAATG GCGACGGTTT CATTATGATA TGAAGATATT TAATCAATGG 
CTAACAGAAG CTGAACACTT TCTCAAAAAG ACACAAATTC CTGAGAACTG GGAACATGCC 
AAATATAAAT GGTATCTTAA GGAACTCCAG GATGGCATCG GGCAGCGGCA AACTGTTGTC 
AGAGTATTGA ATGCAACTGG GGAAGAAATA ATTCAGCAGT CCTCAAAAAT AGATGCCAGT 
ATTCTACAAG AAAAATTGGG AAGCCTGAAT CTGCGGTGGC AGGAGGTCTG CAAACAGCTG 
GCCGAGAGAA AAAAGAGGTT AGAAGAACAA AAGAATATCC TGTCAGAATT TCAAAGAGAT 
TTGAATGAAT TTGTTTTATG GTTGGAAGAA GCAGGTAACA TTTCTAGTAT TCCTCTTGAA 
CCTGGAAATG AGCAGCAACT AAAAGAAAAG CTTGAGGAAG TCAAGTTACT GGCCGAAGAG 
TTGCCCCTGC GTCAGGGAAT TCTAAAACAA TTAAATGAAA CAGGAGGAAC AGTGCTTGTA 
AGTGCTCCCA TAAGCCCAGA AGAGCAAGAT AAACTTGAAA ATAAGCTCAA GCAGACAAAT 
CTTCAGTGGA TAAAGGTTTC TAGGAGTTTA CCTGAGAAAC AGGGAGAAAT TGAGGCTCAC 
ATAAAAGACC TTGGACAGCT TGAAGAGCAA TTAAATCATC TGCTTCTGTG GCTGTCTCCT 
ATTAGGAGTC AGTTGGAAAT TTACAACCAA CCAAATCAGA CAGGACCATT TGACATTAAG 
GAAACTGAAG TAGCAGTTCA AGCTAAACAG CCGGATGTGG AAGGGATTTT GTCTAAAGGG 
CAGAATTTGT ACAAGGAAAA ACCAGCCACT GAGCCAGTGA AGAGGAAGTT AGAAGATCTG 
AGCTCGGAGT GGAAGGCGGT AACCCACTTA CTTCAAGAGC TGAGGGCAAA GTGGCCTGGC 
CCAGTCCCTG GACTGACAGC AACTGGAGCA CCTCCTAGTC AGACGGTTGC TCTGGTGACA 
CAACCTGTGG TTGCCAAGGA AACCGCTGTC TCCAAACTAG AAATGCCATC TTCCTTGCTG 
TTGGAGGTCC CTGCACTGGC AGATTTCAAC CGGGCTTGGA CAGAACTTAC CGACTGGCTG 
TCTCTGCTTG ATCGAGTTCT GAAAGCACAG AGGGTGATGG TGGGTGATCT TGAAGACATC 
AACGAGATGA TCATCAAGCA GAAGGCAACA CTGCAGGACT TGGAGCAGAG GCGCCCCCAG 
TTGGAAGAAC TCATTACTGC TGCCCAGAAT TTGAAAAACA AGACCAGCAA TCAAGAGGCC 
AGGACCATCA TTACTGATCG AATTGAAAGA ATTCAGAGTC AGTGGGATGA AGTACAGGAA 
CACCTTCAGA ACCGGAGGCA GCAGTTAAAT GAAATGTTAA AGGATTCAAC ACAATGGCTG 
GAAGCTAAGG AAGAAGCTGA GCAGGTGGTG GGGCAGGCCA GAGCCAAGCT TGAGACATGG 
AAGGAGGGTC CCTACACAAT GGATGCAATC CAAAGGAAGA TCACAGAAAC CAAGCAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTGG CAAATGACTT GGCACTGAAA 
CTTCTCCGGG ATTATTCTGC AGATGATACC CGAAAGGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT TCATAAAAGA GTGAGTGAGC GAGAGACTGC TCTGGAAGAA 
ACTCATAGAT TACTGCAACA GTTCCCCCTG GACTTGGAGA AGTTTCTTGC CTGGCTTACA 
GAAGCTGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GACTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAGG ACCTCCAAGG AGAAATTGAA 
GCTCACACAG ATATCTATCA CAACCTAGAT GAAAATGGCC AAAAAATCCT GAGATCCCTG 
GAAGGTTCTG ATGACGCGGT CCTATTACAA AGACGTTTGG ATAATATGAA TTTCAAGTGG 
AGTGAGCTGC GGAAAAAGTC TCTCAATATC AGGTCCCATT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTGT GGCTACAGCT GAAAGACGAT 
GAGTTGAGCC GTCAGGCACC TATTGGAGGC GATTTCCCAG CAGTTCAGAA GCAGAATGAT 
ATACACAGGG CCTTCAAGAG GGAACTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTGGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCGCC TGAGGAGAAA GCCCAGAATG TCACACGACT CCTACGAAAG 
CAGGCTGAGG AGGTCAACAC AGAGTGGGAA AAATTGAACC TACACTCGGC TGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAAGAACTGC AGGAGGCCAC GGACGAGCTG 
GACCTCAAAC TGCGCCAAGC TGAGGTGATC AAGGGATCAT GGCAGCCCGT GGGCGATCTT 
CTCATTGACT CCCTCCAGGA TCACCTCGAG AAAGTCAAGG CACTTCGAGG AGAAATTGCA 
CCTCTCAAAG AGAATGTAAG CCACGTCAGT GACCTTGCTC GCCAACTCAC CACGTTGGGC 
ATTCAGCTTT CACCATATAA TCTCAGCACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CTGTTGAGGA CCGCATCAGG CAGCTGCATG AAGCCCACAG GGACTTTGGC 
CCAGCGTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCT 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCGGCTTAT 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT TTTGAGCCTG 
TCAGCCGCAT GTGATGCCTT GGACCAGCAC AACCTCAAGC AAAACGACCA GCCCATGGAC 
ATCCTGCAGA TCATTAACTG TTTGACCACT ATTTATGACC GCCTGGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GATACGGGAC GAACAGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCGC ATTTGGAAGA CAAGTACAGA TACCTTTTCA AGCAAGTGGC CAGTTCAACA 
GGATTTTGTG ACCAGCGCAG ACTGGGCCTC CTTCTACATG ACTCTATCCA AATCCCAAGA 
CAGTTGGGTG AAGTTGCATC CTTTGGGGGC AGTAACATTG AGCCAAGTGT CAGGAGCTGC 
TTCCAATTCG CTAATAATAA GCCTGAGATC GAAGCAGCTC TCTTCCTCGA TTGGATGAGA 
CTAGAACCCC AGTCCATGGT GTGGCTGCCC GTCCTGCACA GGGTGGCTGC CGCGGAGACC 
GCCAAGCATC AGGCCAAGTG TAACATCTGC AAGGAGTGTC CAATCATCGG CTTCAGGTAC 
AGAAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGCT GCTTTTTCTC TGGCCGCGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATATT GTACTCCGAC TACATCGGGA 
GAAGATGTTC GAGACTTTGC CAAGGTACTA AAAAACAAAT TTCGCACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACGG TCTTAGAGGG GGACAACATG 
GAAACGCCTG CCTCGTCCCC TCAGCTTTCA CACGATGATA CTCATTCACG CATTGAACAT 
TATGCTAGCA GGCTAGCAGA AATGGAAAAC AGCAGTGGAT CTTATCTAAA TGATAGCATC 
TCTCCTAATG AGAGCATAGA TGATGAACAT TTGTTAATCC AGCATTACTG CCAAAGTTTG 
AACCAGGACT CCCCCCTGAG CCAGCCTCGT AGTCCTGCCC AGATCTTGAT TTCCTTAGAG 
AGTGAGGAAA GAGGGGAGCT AGAGAGAATC CTAGCAGATC TTGAGGAAGA AAACAGGAAT 
CTGCAGGCTG AATATGATCG TCTCAAGGAG CAGCATGAAC ATAAAGGCCT GTCCCCACTG 
CCGTCCCCTC CTGAGATGAT GCCCACCTCG CCACAGAGTC CCCGGGATGC TGAGCTCATT 
GCTGAGGCCA AGCTACTGCG TCAACACAAA GGCCGCCTGG AAGCCAGGAT GCAAATCCTG 
GAAGACCACA ATAAACAGCT GGAGTCCCAG TTACACAGGC TCAGGCAGCT GCTGGAGCAA 
CCCCAGGCTG AGGCCAAGGT GAATGGCACA ACGGTGTCCT CTCCTTCTAC CTCTCTTCAG 
AGGTCAGACA GCAGCCAGCC TATGCTGCTC CGGGTGGTTG GCAGTCAAAC TTCAGAATCC 
ATGGGCGAGG AAGACCTTCT CAGTCCTCCC CAGGACTCAA GCACAGGGTT AGAGGAAGTG 
ATGGAGCAAC TCAACAACTC CTTCCCTAGT TCCAGAGGAC ACAATGTAGG AAGCCTTTTC 
CACTTGGCAG ATGATTTGGG CAGAGCGATG GAGTCCTTAG TTTCAGTTAT GACAGATGAA 
GAAGGGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844128.1
CDS	251..11305
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALQVLQKNNVDLVNIGSSDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVRQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYVTSLFQVLPQQVSLEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERTPSSPQPRFKSYAYTQAAYVSTSDPTRSPFPSQRLEAPEDKSFGSPLMETEVNLDSYQTALEEVLSWLLSAEDTLQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGSGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNAWLTKTEERTRKMEKEPLGPDLEDLKRQIQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDVLLKWQRFTEEQCLFSTWLSDKEDALNKIPTSGFKDQSEMLSSLQKLAVLKTDLEKKKQTMDKLCSLNHDLLSTLKNTLVAQKMEAWLDNFAQRWDNLVQKLEKSSTQISQAVTTTQPSLTQTTVMETVTMVTTREQILVKHAQEELPPPPPQKKRQIIVDSEIKKRLDVDITELHSWITRSEAVLQSPEFAVYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTPSEPTAVKSQLKNCKDEVNRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADMQAREKELQTILDTLPTVRYQETMSTILTWIQQSETKLSIPQVTVTEYEIMEQRLEELQALQSSLQEHQNDLNYLSTTVKEMSRKAPSHISQRYQSEFENIEGRWKKLSAQLNERCQKLEEQMAKLRKLQNHIKTLKKWMAEVDVFLKDEWPALGDSEILKKQLKQCRLLVSDIQTIQPSLNSVNEGGQKIKTEAEPEFASRLETELRELNTQWDYICRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVQLLTESVNSVIAQAPPAAQEALRKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLNEVELKLKTTENIPGGAEEISEVLSSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLQLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHYQGKETAQRVLSQIEVAQKKMQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDLELTKRSAVEGMPSNLDSEVAWGKATQKETEKQKVHLKSITELGEALKTVLGKKETLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMETFDQNVDHITKWIIQADALLDESEKKKPQQKEDMLKRLKAELNDIRPKVDSTRDQAANLMANRGDHCRKVIEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVHRQAEGLSEDGAAMAVESTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVMVMTEDMPLEISYVPSTYLTEITHVLQALSEVEQLLNAPDLCAKDFQDLFKQEESLKNIKDNLQQISGRIDVIHNKKAAALQSTTPPEKARLQEALSRLDFQWERVNKMYKDRQGQFDRSVEKWRRFHYDMKIFNQWLTEAEHFLKKTQIPENWEHAKYKWYLKELQDGIGQRQTVVRVLNATGEEIIQQSSKIDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDLNEFVLWLEEAGNISSIPLEPGNEQQLKEKLEEVKLLAEELPLRQGILKQLNETGGTVLVSAPISPEEQDKLENKLKQTNLQWIKVSRSLPEKQGEIEAHIKDLGQLEEQLNHLLLWLSPIRSQLEIYNQPNQTGPFDIKETEVAVQAKQPDVEGILSKGQNLYKEKPATEPVKRKLEDLSSEWKAVTHLLQELRAKWPGPVPGLTATGAPPSQTVALVTQPVVAKETAVSKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVLKAQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVVGQARAKLETWKEGPYTMDAIQRKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRVSERETALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKILRSLEGSDDAVLLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDIHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEEKAQNVTRLLRKQAEEVNTEWEKLNLHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVSHVSDLARQLTTLGIQLSPYNLSTLEDLNTRWKLLQVAVEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPASSPQLSHDDTHSRIEHYASRLAEMENSSGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKEQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDSSTGLEEVMEQLNNSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_024988360.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophin (DMD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988360.1

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAATTTTCT AAGTTCGGGA AGCAGCACAT AGAAAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTC TTGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCAGGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAATA TCGGAAGCTC TGACATAGTA 
GATGGAAATC ATAAACTGAC TCTTGGCTTG ATTTGGAATA TAATCCTCCA TTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA GAAGATACTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT CAACTTCACC 
ACCAGCTGGT CTGACGGTTT GGCTTTGAAT GCGCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TCGCCAGCAG TCAGCTACAC AACGACTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTACTGG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TACGTCACAT CTCTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCCTTGAAGC CATCCAGGAG GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACAAGAGAAG AACATTTTCA ACTACATCAT CAAATGCACT ACTCTCAACA GATCACTGTC 
AGTCTAGCAC AGGGCTATGA ACGAACTCCT TCCTCTCCTC AGCCTCGCTT CAAGAGCTAT 
GCCTACACGC AGGCTGCTTA TGTCTCCACC TCTGACCCCA CACGGAGCCC ATTTCCTTCA 
CAGCGTTTGG AAGCTCCTGA AGACAAGTCA TTTGGCAGTC CATTGATGGA GACTGAAGTA 
AACCTGGACA GTTACCAGAC AGCTTTAGAA GAAGTACTCT CATGGCTCCT TTCAGCCGAA 
GACACATTGC AAGCACAAGG AGAGATTTCT AATGATGTAG AAGAAGTGAA AGAACAGTTT 
CATACCCACG AGGGATACAT GATGGATTTG ACATCCCATC AGGGACGGGT TGGCAATGTT 
CTCCAATTGG GGAGTCAGCT GATTGGGTCA GGGAAATTAT CAGAAGATGA AGAAACTGAA 
GTGCAAGAAC AAATGAATCT TCTAAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTC ATGGATCTCC AGAATCAGCA GCTGAAAGAG 
TTAAATGCCT GGTTAACAAA AACAGAAGAG AGAACAAGGA AAATGGAAAA AGAGCCCCTT 
GGACCTGATC TTGAGGACCT CAAACGCCAA ATACAGCAAC ATAAGGTGCT TCAAGAAGAC 
CTAGAACAGG AACAAGTCAG AGTCAATTCT CTCACGCACA TGGTGGTGGT TGTAGATGAG 
TCTAGTGGTG ATCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGG 
TGGGCAAACA TCTGTAGGTG GACTGAAGAT CGCTGGGTTC TTTTACAGGA TGTTCTTCTA 
AAATGGCAAC GTTTTACTGA AGAGCAGTGC CTTTTTAGTA CATGGCTTTC AGATAAAGAA 
GATGCATTGA ACAAGATTCC CACAAGTGGC TTTAAGGATC AAAGTGAAAT GTTATCAAGT 
CTTCAAAAAC TGGCCGTTTT AAAAACTGAT CTAGAAAAGA AAAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCATGA TCTCCTTTCA ACACTGAAAA ATACATTGGT TGCCCAAAAG 
ATGGAAGCAT GGTTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA AAAACTTGAA 
AAGAGTTCAA CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACGGTAAC TATGGTGACC ACGAGGGAGC AAATCCTGGT AAAGCATGCC 
CAAGAGGAAC TCCCTCCACC ACCTCCACAA AAGAAGAGGC AGATTATTGT GGATTCTGAA 
ATTAAGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCCGTGTTAC AGAGTCCTGA ATTTGCAGTC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGTATCAAAC AAGCCTCAGA ACAACTGAAC AGCCGGTGGA TCGAGTTCTG CCAATTGCTA 
AGTGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCA GAAAACTGGT TGAAAACTCA ACCCACTACC 
CCATCAGAGC CAACAGCAGT TAAAAGCCAG TTAAAAAATT GTAAGGATGA AGTCAACCGG 
CTGTCAGCTC TTCAGCCTCA AATCGAGAGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCC GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATATGCA GGCCAGAGAG AAAGAGTTAC AGACAATTTT GGACACTTTA 
CCCACCGTGC GGTATCAGGA GACCATGAGT ACCATCCTCA CGTGGATCCA ACAGTCAGAA 
ACCAAACTCT CGATACCTCA GGTTACTGTC ACTGAATACG AGATCATGGA GCAGAGACTT 
GAGGAGTTAC AGGCTTTACA AAGCTCTCTG CAAGAGCACC AAAATGACCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAGGAAA GCACCCTCTC ATATAAGCCA GAGATATCAG 
TCAGAATTTG AAAACATTGA GGGTCGGTGG AAGAAACTCT CTGCCCAGCT GAATGAACGT 
TGCCAAAAGC TAGAGGAGCA AATGGCCAAA CTCCGAAAAC TTCAGAATCA CATAAAAACA 
CTGAAGAAGT GGATGGCTGA AGTTGATGTT TTCTTGAAAG ATGAATGGCC TGCCCTTGGA 
GATTCAGAAA TTCTTAAAAA ACAGCTGAAG CAGTGCAGGC TTTTAGTCAG TGATATTCAG 
ACAATCCAGC CCAGTCTAAA CAGTGTCAAT GAAGGTGGAC AGAAAATAAA AACTGAAGCG 
GAGCCAGAGT TTGCTTCTAG ACTGGAGACA GAGCTCAGAG AACTTAACAC TCAATGGGAT 
TACATATGTC GACAGGTCTA TGCCAGAAAG GAGGCCTTAA AGGGAGGCTT GGATAAAACT 
GTAAGCCTTC AGAAAGATTT GTCAGAGATG CATGAATGGA TGACACAAGC CGAAGAAGAA 
TACCTAGAGA GAGATTTTGA ATATAAAACC CCAGATGAAT TACAGACAGC AGTTGAAGAG 
ATGAAGAGAG CTAAAGAAGA GGCCCAGCAA AAAGAAGCAA AAGTGCAGCT CCTCACTGAG 
TCCGTGAACA GTGTCATAGC TCAAGCTCCA CCTGCAGCAC AAGAGGCCTT AAGAAAGGAA 
CTGGACACTC TGACCACCAA CTACCAGTGG CTCTGCACCA GGCTGAATGG AAAATGCAAG 
ACGTTAGAAG AGGTTTGGGC ATGTTGGCAT GAGTTATTGT CATACTTGGA AAAGGCAAAT 
AAGTGGCTAA ATGAAGTAGA ATTGAAACTT AAGACTACTG AAAATATTCC TGGTGGAGCT 
GAGGAAATCT CTGAGGTGCT CAGTTCACTT GAAAATTTGA TGCAACATTC AGAAGATAAC 
CCGAATCAGA TTCGCATATT GGCACAGACC CTAACAGATG GTGGAGTCAT GGATGAACTG 
ATCAATGAAG AACTCGAGAC ATTTAATTCT CGTTGGAGAG AACTACATGA AGAGGCTGTG 
AGGAGGCAAA AGTTGCTTGA ACAGAGTATC CAGTCTGCCC AGGAGATTGA AAAATCCTTA 
CAGTTAATTC AGGAATCCCT CTCATCAATT GACAAGCAGT TGGCAGCTTA TATCGCTGAC 
AAAGTGGACG CCGCTCAGAT GCCTCAGGAA GCCCAGAAAA TCCAATCTGA TTTGACAAGT 
CATGAGATCA GTTTAGAAGA AATGAAGAAA CATTACCAAG GAAAGGAAAC TGCTCAACGG 
GTACTATCCC AAATTGAAGT GGCACAGAAA AAAATGCAAG ATGTTTCCAT GAAATTTCGA 
TTGTTCCAGA AGCCAGCCAA TTTTGAGCAG CGTCTACAGG AAAGTAAGAT GATTTTAGAT 
GAAGTGAAGA TGCACTTGCC CGCACTGGAA ACAAAGAGTG TTGAACAGGA AGTAGTACAG 
TCACAGTTAA ATCATTGTGT GAACTTGTAT AAAAGTCTGA GTGAAGTGAA GTCTGAAGTG 
GAAATGGTGA TTAAAACTGG ACGTCAAATT GTACAGAAGA AGCAGACGGA AAACCCTAAA 
GAGCTTGATG AAAGAGTAAC GGCTTTGAAA TTGCATTATA ATGAGCTGGG AGCAAAGGTA 
ACAGAAAGAA AGCAACAGTT GGAGAAATGC TTGAAATTGT CCCGTAAGAT GCGAAAGGAA 
ATGAATGCCT TGACAGAATG GCTGGCAGCT ACAGATTTGG AACTGACAAA GAGATCAGCA 
GTTGAAGGAA TGCCTAGTAA TTTGGATTCT GAAGTTGCCT GGGGAAAGGC TACTCAGAAA 
GAGACTGAGA AACAGAAGGT TCACCTAAAG AGCATTACAG AGCTAGGAGA GGCCTTGAAA 
ACGGTTTTGG GCAAGAAGGA GACCTTGGTC GAAGATAAAC TGAGTCTTTT GAATAGTAAC 
TGGATAGCCG TCACTTCTCG AGCAGAAGAG TGGCTAAACC TTTTGTTGGA ATACCAGAAA 
CACATGGAAA CCTTTGACCA GAATGTGGAT CATATCACAA AGTGGATCAT TCAAGCTGAT 
GCACTTCTGG ATGAATCTGA GAAAAAGAAA CCACAGCAAA AAGAAGACAT GCTAAAGCGC 
TTAAAGGCTG AACTGAATGA CATACGCCCA AAGGTGGACT CCACGCGTGA CCAAGCAGCA 
AACTTGATGG CAAACCGCGG CGACCACTGC AGGAAAGTAA TAGAGCCCAA AATCTCAGAG 
CTCAACCATC GATTTGCAGC CATTTCTCAC AGAATTAAGA CTGGAAAGGC CTCCATTCCT 
TTGAAGGAAT TGGAGCAGTT TAACTCAGAT ATACAAAAAT TGCTTGAACC ACTGGAGGCT 
GAAATTCAGC AGGGGGTGAA TCTGAAAGAG GAAGACTTCA ATAAAGATAT GAGTGAAGAC 
AATGAGGGTA CTGTAAAAGA ATTGTTGCAA AGAGGAGACA ACTTACAACA AAGAATCACA 
GATGAGAGAA AGCGAGAGGA AATAAAGATA AAACAGCAGC TGTTACAGAC AAAACATAAT 
GCTCTCAAGG ATTTGAGGTC TCAAAGAAGA AAAAAGGCTC TAGAAATTTC TCACCAGTGG 
TATCAGTACA AGAGGCAGGC TGATGATCTC CTGAAATGCT TGGATGACAT TGAAAAAAAA 
TTAGCCAGCC TACCTGAACC CAGAGATGAA AGGAAAATAA AGGAAATTGA TCGTGAGTTG 
CAGAAGAAGA AAGAGGAGCT GAATGCAGTG CATAGGCAAG CTGAGGGCTT GTCTGAGGAT 
GGGGCAGCAA TGGCAGTGGA GTCAACTCAG ATCCAGCTCA GCAAGCGCTG GCGGGAAATT 
GAGAGCAAAT TTGCTCAGTT TCGAAGACTC AACTTTGCAC AAATTCACAC TGTCCATGAA 
GAATCAGTGA TGGTGATGAC CGAAGACATG CCTTTGGAAA TTTCTTATGT GCCTTCTACT 
TACCTGACTG AGATCACTCA CGTCTTACAA GCCCTTTCAG AAGTGGAACA ACTTCTCAAT 
GCTCCCGACC TCTGTGCTAA AGATTTTCAA GATCTCTTCA AACAAGAGGA ATCTCTGAAG 
AATATAAAAG ACAACCTGCA ACAAATCTCA GGTCGGATTG ATGTTATTCA CAATAAGAAG 
GCGGCAGCAT TGCAAAGCAC CACACCCCCA GAAAAGGCAA GGTTACAGGA AGCTCTCTCC 
AGGCTCGACT TCCAATGGGA AAGAGTGAAC AAAATGTACA AGGACCGACA AGGGCAATTT 
GACAGATCTG TTGAAAAATG GCGACGGTTT CATTATGATA TGAAGATATT TAATCAATGG 
CTAACAGAAG CTGAACACTT TCTCAAAAAG ACACAAATTC CTGAGAACTG GGAACATGCC 
AAATATAAAT GGTATCTTAA GGAACTCCAG GATGGCATCG GGCAGCGGCA AACTGTTGTC 
AGAGTATTGA ATGCAACTGG GGAAGAAATA ATTCAGCAGT CCTCAAAAAT AGATGCCAGT 
ATTCTACAAG AAAAATTGGG AAGCCTGAAT CTGCGGTGGC AGGAGGTCTG CAAACAGCTG 
GCCGAGAGAA AAAAGAGGTT AGAAGAACAA AAGAATATCC TGTCAGAATT TCAAAGAGAT 
TTGAATGAAT TTGTTTTATG GTTGGAAGAA GCAGGTAACA TTTCTAGTAT TCCTCTTGAA 
CCTGGAAATG AGCAGCAACT AAAAGAAAAG CTTGAGGAAG TCAAGTTACT GGCCGAAGAG 
TTGCCCCTGC GTCAGGGAAT TCTAAAACAA TTAAATGAAA CAGGAGGAAC AGTGCTTGTA 
AGTGCTCCCA TAAGCCCAGA AGAGCAAGAT AAACTTGAAA ATAAGCTCAA GCAGACAAAT 
CTTCAGTGGA TAAAGGTTTC TAGGAGTTTA CCTGAGAAAC AGGGAGAAAT TGAGGCTCAC 
ATAAAAGACC TTGGACAGCT TGAAGAGCAA TTAAATCATC TGCTTCTGTG GCTGTCTCCT 
ATTAGGAGTC AGTTGGAAAT TTACAACCAA CCAAATCAGA CAGGACCATT TGACATTAAG 
GAAACTGAAG TAGCAGTTCA AGCTAAACAG CCGGATGTGG AAGGGATTTT GTCTAAAGGG 
CAGAATTTGT ACAAGGAAAA ACCAGCCACT GAGCCAGTGA AGAGGAAGTT AGAAGATCTG 
AGCTCGGAGT GGAAGGCGGT AACCCACTTA CTTCAAGAGC TGAGGGCAAA GTGGCCTGGC 
CCAGTCCCTG GACTGACAGC AACTGGAGCA CCTCCTAGTC AGACGGTTGC TCTGGTGACA 
CAACCTGTGG TTGCCAAGGA AACCGCTGTC TCCAAACTAG AAATGCCATC TTCCTTGCTG 
TTGGAGGTCC CTGCACTGGC AGATTTCAAC CGGGCTTGGA CAGAACTTAC CGACTGGCTG 
TCTCTGCTTG ATCGAGTTCT GAAAGCACAG AGGGTGATGG TGGGTGATCT TGAAGACATC 
AACGAGATGA TCATCAAGCA GAAGGCAACA CTGCAGGACT TGGAGCAGAG GCGCCCCCAG 
TTGGAAGAAC TCATTACTGC TGCCCAGAAT TTGAAAAACA AGACCAGCAA TCAAGAGGCC 
AGGACCATCA TTACTGATCG AATTGAAAGA ATTCAGAGTC AGTGGGATGA AGTACAGGAA 
CACCTTCAGA ACCGGAGGCA GCAGTTAAAT GAAATGTTAA AGGATTCAAC ACAATGGCTG 
GAAGCTAAGG AAGAAGCTGA GCAGGTGGTG GGGCAGGCCA GAGCCAAGCT TGAGACATGG 
AAGGAGGGTC CCTACACAAT GGATGCAATC CAAAGGAAGA TCACAGAAAC CAAGCAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTGG CAAATGACTT GGCACTGAAA 
CTTCTCCGGG ATTATTCTGC AGATGATACC CGAAAGGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT TCATAAAAGA GTGAGTGAGC GAGAGACTGC TCTGGAAGAA 
ACTCATAGAT TACTGCAACA GTTCCCCCTG GACTTGGAGA AGTTTCTTGC CTGGCTTACA 
GAAGCTGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GACTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAGG ACCTCCAAGG AGAAATTGAA 
GCTCACACAG ATATCTATCA CAACCTAGAT GAAAATGGCC AAAAAATCCT GAGATCCCTG 
GAAGGTTCTG ATGACGCGGT CCTATTACAA AGACGTTTGG ATAATATGAA TTTCAAGTGG 
AGTGAGCTGC GGAAAAAGTC TCTCAATATC AGGTCCCATT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTGT GGCTACAGCT GAAAGACGAT 
GAGTTGAGCC GTCAGGCACC TATTGGAGGC GATTTCCCAG CAGTTCAGAA GCAGAATGAT 
ATACACAGGG CCTTCAAGAG GGAACTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTGGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCGCC TGAGGAGAAA GCCCAGAATG TCACACGACT CCTACGAAAG 
CAGGCTGAGG AGGTCAACAC AGAGTGGGAA AAATTGAACC TACACTCGGC TGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAAGAACTGC AGGAGGCCAC GGACGAGCTG 
GACCTCAAAC TGCGCCAAGC TGAGGTGATC AAGGGATCAT GGCAGCCCGT GGGCGATCTT 
CTCATTGACT CCCTCCAGGA TCACCTCGAG AAAGTCAAGG CACTTCGAGG AGAAATTGCA 
CCTCTCAAAG AGAATGTAAG CCACGTCAGT GACCTTGCTC GCCAACTCAC CACGTTGGGC 
ATTCAGCTTT CACCATATAA TCTCAGCACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CTGTTGAGGA CCGCATCAGG CAGCTGCATG AAGCCCACAG GGACTTTGGC 
CCAGCGTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCT 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCGGCTTAT 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT TTTGAGCCTG 
TCAGCCGCAT GTGATGCCTT GGACCAGCAC AACCTCAAGC AAAACGACCA GCCCATGGAC 
ATCCTGCAGA TCATTAACTG TTTGACCACT ATTTATGACC GCCTGGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GATACGGGAC GAACAGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCGC ATTTGGAAGA CAAGTACAGA TACCTTTTCA AGCAAGTGGC CAGTTCAACA 
GGATTTTGTG ACCAGCGCAG ACTGGGCCTC CTTCTACATG ACTCTATCCA AATCCCAAGA 
CAGTTGGGTG AAGTTGCATC CTTTGGGGGC AGTAACATTG AGCCAAGTGT CAGGAGCTGC 
TTCCAATTCG CTAATAATAA GCCTGAGATC GAAGCAGCTC TCTTCCTCGA TTGGATGAGA 
CTAGAACCCC AGTCCATGGT GTGGCTGCCC GTCCTGCACA GGGTGGCTGC CGCGGAGACC 
GCCAAGCATC AGGCCAAGTG TAACATCTGC AAGGAGTGTC CAATCATCGG CTTCAGGTAC 
AGAAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGCT GCTTTTTCTC TGGCCGCGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATATT GTACTCCGAC TACATCGGGA 
GAAGATGTTC GAGACTTTGC CAAGGTACTA AAAAACAAAT TTCGCACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACGG TCTTAGAGGG GGACAACATG 
GAAACGCCTG CCTCGTCCCC TCAGCTTTCA CACGATGATA CTCATTCACG CATTGAACAT 
TATGCTAGCA GGCTAGCAGA AATGGAAAAC AGCAGTGGAT CTTATCTAAA TGATAGCATC 
TCTCCTAATG AGAGCATAGA TGATGAACAT TTGTTAATCC AGCATTACTG CCAAAGTTTG 
AACCAGGACT CCCCCCTGAG CCAGCCTCGT AGTCCTGCCC AGATCTTGAT TTCCTTAGAG 
AGTGAGGAAA GAGGGGAGCT AGAGAGAATC CTAGCAGATC TTGAGGAAGA AAACAGGAAT 
CTGCAGGCTG AATATGATCG TCTCAAGGAG CAGCATGAAC ATAAAGGCCT GTCCCCACTG 
CCGTCCCCTC CTGAGATGAT GCCCACCTCG CCACAGAGTC CCCGGGATGC TGAGCTCATT 
GCTGAGGCCA AGCTACTGCG TCAACACAAA GGCCGCCTGG AAGCCAGGAT GCAAATCCTG 
GAAGACCACA ATAAACAGCT GGAGTCCCAG TTACACAGGC TCAGGCAGCT GCTGGAGCAA 
CCCCAGGCTG AGGCCAAGGT GAATGGCACA ACGGTGTCCT CTCCTTCTAC CTCTCTTCAG 
AGGTCAGACA GCAGCCAGCC TATGCTGCTC CGGGTGGTTG GCAGTCAAAC TTCAGAATCC 
ATGGGCGAGG AAGACCTTCT CAGTCCTCCC CAGGACTCAA GCACAGGGTT AGAGGAAGTG 
ATGGAGCAAC TCAACAACTC CTTCCCTAGT TCCAGAGGAC ACAATGTAGG AAGCCTTTTC 
CACTTGGCAG ATGATTTGGG CAGAGCGATG GAGTCCTTAG TTTCAGTTAT GACAGATGAA 
GAAGGGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_024988361.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844129.1 Bos taurus dystrophin (DMD), transcript variant X4, mRNA.

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CloneID	OBa238540
Clone ID Related Accession (Same CDS sequence)	XM_024988361.1
Accession Version	XM_024988361.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAATTTTCT AAGTTCGGGA AGCAGCACAT AGAAAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTC TTGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCAGGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAATA TCGGAAGCTC TGACATAGTA 
GATGGAAATC ATAAACTGAC TCTTGGCTTG ATTTGGAATA TAATCCTCCA TTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA GAAGATACTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT CAACTTCACC 
ACCAGCTGGT CTGACGGTTT GGCTTTGAAT GCGCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TCGCCAGCAG TCAGCTACAC AACGACTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTACTGG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TACGTCACAT CTCTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCCTTGAAGC CATCCAGGAG GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACAAGAGAAG AACATTTTCA ACTACATCAT CAAATGCACT ACTCTCAACA GATCACTGTC 
AGTCTAGCAC AGGGCTATGA ACGAACTCCT TCCTCTCCTC AGCCTCGCTT CAAGAGCTAT 
GCCTACACGC AGGCTGCTTA TGTCTCCACC TCTGACCCCA CACGGAGCCC ATTTCCTTCA 
CAGCGTTTGG AAGCTCCTGA AGACAAGTCA TTTGGCAGTC CATTGATGGA GACTGAAGTA 
AACCTGGACA GTTACCAGAC AGCTTTAGAA GAAGTACTCT CATGGCTCCT TTCAGCCGAA 
GACACATTGC AAGCACAAGG AGAGATTTCT AATGATGTAG AAGAAGTGAA AGAACAGTTT 
CATACCCACG AGGGATACAT GATGGATTTG ACATCCCATC AGGGACGGGT TGGCAATGTT 
CTCCAATTGG GGAGTCAGCT GATTGGGTCA GGGAAATTAT CAGAAGATGA AGAAACTGAA 
GTGCAAGAAC AAATGAATCT TCTAAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTC ATGGATCTCC AGAATCAGCA GCTGAAAGAG 
TTAAATGCCT GGTTAACAAA AACAGAAGAG AGAACAAGGA AAATGGAAAA AGAGCCCCTT 
GGACCTGATC TTGAGGACCT CAAACGCCAA ATACAGCAAC ATAAGGTGCT TCAAGAAGAC 
CTAGAACAGG AACAAGTCAG AGTCAATTCT CTCACGCACA TGGTGGTGGT TGTAGATGAG 
TCTAGTGGTG ATCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGG 
TGGGCAAACA TCTGTAGGTG GACTGAAGAT CGCTGGGTTC TTTTACAGGA TGTTCTTCTA 
AAATGGCAAC GTTTTACTGA AGAGCAGTGC CTTTTTAGTA CATGGCTTTC AGATAAAGAA 
GATGCATTGA ACAAGATTCC CACAAGTGGC TTTAAGGATC AAAGTGAAAT GTTATCAAGT 
CTTCAAAAAC TGGCCGTTTT AAAAACTGAT CTAGAAAAGA AAAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCATGA TCTCCTTTCA ACACTGAAAA ATACATTGGT TGCCCAAAAG 
ATGGAAGCAT GGTTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA AAAACTTGAA 
AAGAGTTCAA CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACGGTAAC TATGGTGACC ACGAGGGAGC AAATCCTGGT AAAGCATGCC 
CAAGAGGAAC TCCCTCCACC ACCTCCACAA AAGAAGAGGC AGATTATTGT GGATTCTGAA 
ATTAAGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCCGTGTTAC AGAGTCCTGA ATTTGCAGTC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGTATCAAAC AAGCCTCAGA ACAACTGAAC AGCCGGTGGA TCGAGTTCTG CCAATTGCTA 
AGTGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCA GAAAACTGGT TGAAAACTCA ACCCACTACC 
CCATCAGAGC CAACAGCAGT TAAAAGCCAG TTAAAAAATT GTAAGGATGA AGTCAACCGG 
CTGTCAGCTC TTCAGCCTCA AATCGAGAGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCC GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATATGCA GGCCAGAGAG AAAGAGTTAC AGACAATTTT GGACACTTTA 
CCCACCGTGC GGTATCAGGA GACCATGAGT ACCATCCTCA CGTGGATCCA ACAGTCAGAA 
ACCAAACTCT CGATACCTCA GGTTACTGTC ACTGAATACG AGATCATGGA GCAGAGACTT 
GAGGAGTTAC AGGCTTTACA AAGCTCTCTG CAAGAGCACC AAAATGACCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAGGAAA GCACCCTCTC ATATAAGCCA GAGATATCAG 
TCAGAATTTG AAAACATTGA GGGTCGGTGG AAGAAACTCT CTGCCCAGCT GAATGAACGT 
TGCCAAAAGC TAGAGGAGCA AATGGCCAAA CTCCGAAAAC TTCAGAATCA CATAAAAACA 
CTGAAGAAGT GGATGGCTGA AGTTGATGTT TTCTTGAAAG ATGAATGGCC TGCCCTTGGA 
GATTCAGAAA TTCTTAAAAA ACAGCTGAAG CAGTGCAGGC TTTTAGTCAG TGATATTCAG 
ACAATCCAGC CCAGTCTAAA CAGTGTCAAT GAAGGTGGAC AGAAAATAAA AACTGAAGCG 
GAGCCAGAGT TTGCTTCTAG ACTGGAGACA GAGCTCAGAG AACTTAACAC TCAATGGGAT 
TACATATGTC GACAGGTCTA TGCCAGAAAG GAGGCCTTAA AGGGAGGCTT GGATAAAACT 
GTAAGCCTTC AGAAAGATTT GTCAGAGATG CATGAATGGA TGACACAAGC CGAAGAAGAA 
TACCTAGAGA GAGATTTTGA ATATAAAACC CCAGATGAAT TACAGACAGC AGTTGAAGAG 
ATGAAGAGAG CTAAAGAAGA GGCCCAGCAA AAAGAAGCAA AAGTGCAGCT CCTCACTGAG 
TCCGTGAACA GTGTCATAGC TCAAGCTCCA CCTGCAGCAC AAGAGGCCTT AAGAAAGGAA 
CTGGACACTC TGACCACCAA CTACCAGTGG CTCTGCACCA GGCTGAATGG AAAATGCAAG 
ACGTTAGAAG AGGTTTGGGC ATGTTGGCAT GAGTTATTGT CATACTTGGA AAAGGCAAAT 
AAGTGGCTAA ATGAAGTAGA ATTGAAACTT AAGACTACTG AAAATATTCC TGGTGGAGCT 
GAGGAAATCT CTGAGGTGCT CAGTTCACTT GAAAATTTGA TGCAACATTC AGAAGATAAC 
CCGAATCAGA TTCGCATATT GGCACAGACC CTAACAGATG GTGGAGTCAT GGATGAACTG 
ATCAATGAAG AACTCGAGAC ATTTAATTCT CGTTGGAGAG AACTACATGA AGAGGCTGTG 
AGGAGGCAAA AGTTGCTTGA ACAGAGTATC CAGTCTGCCC AGGAGATTGA AAAATCCTTA 
CAGTTAATTC AGGAATCCCT CTCATCAATT GACAAGCAGT TGGCAGCTTA TATCGCTGAC 
AAAGTGGACG CCGCTCAGAT GCCTCAGGAA GCCCAGAAAA TCCAATCTGA TTTGACAAGT 
CATGAGATCA GTTTAGAAGA AATGAAGAAA CATTACCAAG GAAAGGAAAC TGCTCAACGG 
GTACTATCCC AAATTGAAGT GGCACAGAAA AAAATGCAAG ATGTTTCCAT GAAATTTCGA 
TTGTTCCAGA AGCCAGCCAA TTTTGAGCAG CGTCTACAGG AAAGTAAGAT GATTTTAGAT 
GAAGTGAAGA TGCACTTGCC CGCACTGGAA ACAAAGAGTG TTGAACAGGA AGTAGTACAG 
TCACAGTTAA ATCATTGTGT GAACTTGTAT AAAAGTCTGA GTGAAGTGAA GTCTGAAGTG 
GAAATGGTGA TTAAAACTGG ACGTCAAATT GTACAGAAGA AGCAGACGGA AAACCCTAAA 
GAGCTTGATG AAAGAGTAAC GGCTTTGAAA TTGCATTATA ATGAGCTGGG AGCAAAGGTA 
ACAGAAAGAA AGCAACAGTT GGAGAAATGC TTGAAATTGT CCCGTAAGAT GCGAAAGGAA 
ATGAATGCCT TGACAGAATG GCTGGCAGCT ACAGATTTGG AACTGACAAA GAGATCAGCA 
GTTGAAGGAA TGCCTAGTAA TTTGGATTCT GAAGTTGCCT GGGGAAAGGC TACTCAGAAA 
GAGACTGAGA AACAGAAGGT TCACCTAAAG AGCATTACAG AGCTAGGAGA GGCCTTGAAA 
ACGGTTTTGG GCAAGAAGGA GACCTTGGTC GAAGATAAAC TGAGTCTTTT GAATAGTAAC 
TGGATAGCCG TCACTTCTCG AGCAGAAGAG TGGCTAAACC TTTTGTTGGA ATACCAGAAA 
CACATGGAAA CCTTTGACCA GAATGTGGAT CATATCACAA AGTGGATCAT TCAAGCTGAT 
GCACTTCTGG ATGAATCTGA GAAAAAGAAA CCACAGCAAA AAGAAGACAT GCTAAAGCGC 
TTAAAGGCTG AACTGAATGA CATACGCCCA AAGGTGGACT CCACGCGTGA CCAAGCAGCA 
AACTTGATGG CAAACCGCGG CGACCACTGC AGGAAAGTAA TAGAGCCCAA AATCTCAGAG 
CTCAACCATC GATTTGCAGC CATTTCTCAC AGAATTAAGA CTGGAAAGGC CTCCATTCCT 
TTGAAGGAAT TGGAGCAGTT TAACTCAGAT ATACAAAAAT TGCTTGAACC ACTGGAGGCT 
GAAATTCAGC AGGGGGTGAA TCTGAAAGAG GAAGACTTCA ATAAAGATAT GAGTGAAGAC 
AATGAGGGTA CTGTAAAAGA ATTGTTGCAA AGAGGAGACA ACTTACAACA AAGAATCACA 
GATGAGAGAA AGCGAGAGGA AATAAAGATA AAACAGCAGC TGTTACAGAC AAAACATAAT 
GCTCTCAAGG ATTTGAGGTC TCAAAGAAGA AAAAAGGCTC TAGAAATTTC TCACCAGTGG 
TATCAGTACA AGAGGCAGGC TGATGATCTC CTGAAATGCT TGGATGACAT TGAAAAAAAA 
TTAGCCAGCC TACCTGAACC CAGAGATGAA AGGAAAATAA AGGAAATTGA TCGTGAGTTG 
CAGAAGAAGA AAGAGGAGCT GAATGCAGTG CATAGGCAAG CTGAGGGCTT GTCTGAGGAT 
GGGGCAGCAA TGGCAGTGGA GTCAACTCAG ATCCAGCTCA GCAAGCGCTG GCGGGAAATT 
GAGAGCAAAT TTGCTCAGTT TCGAAGACTC AACTTTGCAC AAATTCACAC TGTCCATGAA 
GAATCAGTGA TGGTGATGAC CGAAGACATG CCTTTGGAAA TTTCTTATGT GCCTTCTACT 
TACCTGACTG AGATCACTCA CGTCTTACAA GCCCTTTCAG AAGTGGAACA ACTTCTCAAT 
GCTCCCGACC TCTGTGCTAA AGATTTTCAA GATCTCTTCA AACAAGAGGA ATCTCTGAAG 
AATATAAAAG ACAACCTGCA ACAAATCTCA GGTCGGATTG ATGTTATTCA CAATAAGAAG 
GCGGCAGCAT TGCAAAGCAC CACACCCCCA GAAAAGGCAA GGTTACAGGA AGCTCTCTCC 
AGGCTCGACT TCCAATGGGA AAGAGTGAAC AAAATGTACA AGGACCGACA AGGGCAATTT 
GACAGATCTG TTGAAAAATG GCGACGGTTT CATTATGATA TGAAGATATT TAATCAATGG 
CTAACAGAAG CTGAACACTT TCTCAAAAAG ACACAAATTC CTGAGAACTG GGAACATGCC 
AAATATAAAT GGTATCTTAA GGAACTCCAG GATGGCATCG GGCAGCGGCA AACTGTTGTC 
AGAGTATTGA ATGCAACTGG GGAAGAAATA ATTCAGCAGT CCTCAAAAAT AGATGCCAGT 
ATTCTACAAG AAAAATTGGG AAGCCTGAAT CTGCGGTGGC AGGAGGTCTG CAAACAGCTG 
GCCGAGAGAA AAAAGAGGTT AGAAGAACAA AAGAATATCC TGTCAGAATT TCAAAGAGAT 
TTGAATGAAT TTGTTTTATG GTTGGAAGAA GCAGGTAACA TTTCTAGTAT TCCTCTTGAA 
CCTGGAAATG AGCAGCAACT AAAAGAAAAG CTTGAGGAAG TCAAGTTACT GGCCGAAGAG 
TTGCCCCTGC GTCAGGGAAT TCTAAAACAA TTAAATGAAA CAGGAGGAAC AGTGCTTGTA 
AGTGCTCCCA TAAGCCCAGA AGAGCAAGAT AAACTTGAAA ATAAGCTCAA GCAGACAAAT 
CTTCAGTGGA TAAAGGTTTC TAGGAGTTTA CCTGAGAAAC AGGGAGAAAT TGAGGCTCAC 
ATAAAAGACC TTGGACAGCT TGAAGAGCAA TTAAATCATC TGCTTCTGTG GCTGTCTCCT 
ATTAGGAGTC AGTTGGAAAT TTACAACCAA CCAAATCAGA CAGGACCATT TGACATTAAG 
GAAACTGAAG TAGCAGTTCA AGCTAAACAG CCGGATGTGG AAGGGATTTT GTCTAAAGGG 
CAGAATTTGT ACAAGGAAAA ACCAGCCACT GAGCCAGTGA AGAGGAAGTT AGAAGATCTG 
AGCTCGGAGT GGAAGGCGGT AACCCACTTA CTTCAAGAGC TGAGGGCAAA GTGGCCTGGC 
CCAGTCCCTG GACTGACAGC AACTGGAGCA CCTCCTAGTC AGACGGTTGC TCTGGTGACA 
CAACCTGTGG TTGCCAAGGA AACCGCTGTC TCCAAACTAG AAATGCCATC TTCCTTGCTG 
TTGGAGGTCC CTGCACTGGC AGATTTCAAC CGGGCTTGGA CAGAACTTAC CGACTGGCTG 
TCTCTGCTTG ATCGAGTTCT GAAAGCACAG AGGGTGATGG TGGGTGATCT TGAAGACATC 
AACGAGATGA TCATCAAGCA GAAGGCAACA CTGCAGGACT TGGAGCAGAG GCGCCCCCAG 
TTGGAAGAAC TCATTACTGC TGCCCAGAAT TTGAAAAACA AGACCAGCAA TCAAGAGGCC 
AGGACCATCA TTACTGATCG AATTGAAAGA ATTCAGAGTC AGTGGGATGA AGTACAGGAA 
CACCTTCAGA ACCGGAGGCA GCAGTTAAAT GAAATGTTAA AGGATTCAAC ACAATGGCTG 
GAAGCTAAGG AAGAAGCTGA GCAGGTGGTG GGGCAGGCCA GAGCCAAGCT TGAGACATGG 
AAGGAGGGTC CCTACACAAT GGATGCAATC CAAAGGAAGA TCACAGAAAC CAAGCAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTGG CAAATGACTT GGCACTGAAA 
CTTCTCCGGG ATTATTCTGC AGATGATACC CGAAAGGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT TCATAAAAGA GTGAGTGAGC GAGAGACTGC TCTGGAAGAA 
ACTCATAGAT TACTGCAACA GTTCCCCCTG GACTTGGAGA AGTTTCTTGC CTGGCTTACA 
GAAGCTGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GACTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAGG ACCTCCAAGG AGAAATTGAA 
GCTCACACAG ATATCTATCA CAACCTAGAT GAAAATGGCC AAAAAATCCT GAGATCCCTG 
GAAGGTTCTG ATGACGCGGT CCTATTACAA AGACGTTTGG ATAATATGAA TTTCAAGTGG 
AGTGAGCTGC GGAAAAAGTC TCTCAATATC AGGTCCCATT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTGT GGCTACAGCT GAAAGACGAT 
GAGTTGAGCC GTCAGGCACC TATTGGAGGC GATTTCCCAG CAGTTCAGAA GCAGAATGAT 
ATACACAGGG CCTTCAAGAG GGAACTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTGGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCGCC TGAGGAGAAA GCCCAGAATG TCACACGACT CCTACGAAAG 
CAGGCTGAGG AGGTCAACAC AGAGTGGGAA AAATTGAACC TACACTCGGC TGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAAGAACTGC AGGAGGCCAC GGACGAGCTG 
GACCTCAAAC TGCGCCAAGC TGAGGTGATC AAGGGATCAT GGCAGCCCGT GGGCGATCTT 
CTCATTGACT CCCTCCAGGA TCACCTCGAG AAAGTCAAGG CACTTCGAGG AGAAATTGCA 
CCTCTCAAAG AGAATGTAAG CCACGTCAGT GACCTTGCTC GCCAACTCAC CACGTTGGGC 
ATTCAGCTTT CACCATATAA TCTCAGCACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CTGTTGAGGA CCGCATCAGG CAGCTGCATG AAGCCCACAG GGACTTTGGC 
CCAGCGTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCT 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCGGCTTAT 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT TTTGAGCCTG 
TCAGCCGCAT GTGATGCCTT GGACCAGCAC AACCTCAAGC AAAACGACCA GCCCATGGAC 
ATCCTGCAGA TCATTAACTG TTTGACCACT ATTTATGACC GCCTGGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GATACGGGAC GAACAGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCGC ATTTGGAAGA CAAGTACAGA TACCTTTTCA AGCAAGTGGC CAGTTCAACA 
GGATTTTGTG ACCAGCGCAG ACTGGGCCTC CTTCTACATG ACTCTATCCA AATCCCAAGA 
CAGTTGGGTG AAGTTGCATC CTTTGGGGGC AGTAACATTG AGCCAAGTGT CAGGAGCTGC 
TTCCAATTCG CTAATAATAA GCCTGAGATC GAAGCAGCTC TCTTCCTCGA TTGGATGAGA 
CTAGAACCCC AGTCCATGGT GTGGCTGCCC GTCCTGCACA GGGTGGCTGC CGCGGAGACC 
GCCAAGCATC AGGCCAAGTG TAACATCTGC AAGGAGTGTC CAATCATCGG CTTCAGGTAC 
AGAAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGCT GCTTTTTCTC TGGCCGCGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATATT GTACTCCGAC TACATCGGGA 
GAAGATGTTC GAGACTTTGC CAAGGTACTA AAAAACAAAT TTCGCACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACGG TCTTAGAGGG GGACAACATG 
GAAACTCCTG TTACTCTGAT CAACTTCTGG CCGGTAGACT CTGCGCCTGC CTCGTCCCCT 
CAGCTTTCAC ACGATGATAC TCATTCACGC ATTGAACATT ATGCTAGCAG GCTAGCAGAA 
ATGGAAAACA GCAGTGGATC TTATCTAAAT GATAGCATCT CTCCTAATGA GAGCATAGAT 
GATGAACATT TGTTAATCCA GCATTACTGC CAAAGTTTGA ACCAGGACTC CCCCCTGAGC 
CAGCCTCGTA GTCCTGCCCA GATCTTGATT TCCTTAGAGA GTGAGGAAAG AGGGGAGCTA 
GAGAGAATCC TAGCAGATCT TGAGGAAGAA AACAGGAATC TGCAGGCTGA ATATGATCGT 
CTCAAGGAGC AGCATGAACA TAAAGGCCTG TCCCCACTGC CGTCCCCTCC TGAGATGATG 
CCCACCTCGC CACAGAGTCC CCGGGATGCT GAGCTCATTG CTGAGGCCAA GCTACTGCGT 
CAACACAAAG GCCGCCTGGA AGCCAGGATG CAAATCCTGG AAGACCACAA TAAACAGCTG 
GAGTCCCAGT TACACAGGCT CAGGCAGCTG CTGGAGCAAC CCCAGGCTGA GGCCAAGGTG 
AATGGCACAA CGGTGTCCTC TCCTTCTACC TCTCTTCAGA GGTCAGACAG CAGCCAGCCT 
ATGCTGCTCC GGGTGGTTGG CAGTCAAACT TCAGAATCCA TGGGCGAGGA AGACCTTCTC 
AGTCCTCCCC AGGACTCAAG CACAGGGTTA GAGGAAGTGA TGGAGCAACT CAACAACTCC 
TTCCCTAGTT CCAGAGGAAG AAATACCCCT GGGAAGCCAA TGAGAGAGGA CACAATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844129.1
CDS	251..11290
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALQVLQKNNVDLVNIGSSDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVRQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYVTSLFQVLPQQVSLEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERTPSSPQPRFKSYAYTQAAYVSTSDPTRSPFPSQRLEAPEDKSFGSPLMETEVNLDSYQTALEEVLSWLLSAEDTLQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGSGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNAWLTKTEERTRKMEKEPLGPDLEDLKRQIQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDVLLKWQRFTEEQCLFSTWLSDKEDALNKIPTSGFKDQSEMLSSLQKLAVLKTDLEKKKQTMDKLCSLNHDLLSTLKNTLVAQKMEAWLDNFAQRWDNLVQKLEKSSTQISQAVTTTQPSLTQTTVMETVTMVTTREQILVKHAQEELPPPPPQKKRQIIVDSEIKKRLDVDITELHSWITRSEAVLQSPEFAVYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTPSEPTAVKSQLKNCKDEVNRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADMQAREKELQTILDTLPTVRYQETMSTILTWIQQSETKLSIPQVTVTEYEIMEQRLEELQALQSSLQEHQNDLNYLSTTVKEMSRKAPSHISQRYQSEFENIEGRWKKLSAQLNERCQKLEEQMAKLRKLQNHIKTLKKWMAEVDVFLKDEWPALGDSEILKKQLKQCRLLVSDIQTIQPSLNSVNEGGQKIKTEAEPEFASRLETELRELNTQWDYICRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVQLLTESVNSVIAQAPPAAQEALRKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLNEVELKLKTTENIPGGAEEISEVLSSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLQLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHYQGKETAQRVLSQIEVAQKKMQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDLELTKRSAVEGMPSNLDSEVAWGKATQKETEKQKVHLKSITELGEALKTVLGKKETLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMETFDQNVDHITKWIIQADALLDESEKKKPQQKEDMLKRLKAELNDIRPKVDSTRDQAANLMANRGDHCRKVIEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVHRQAEGLSEDGAAMAVESTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVMVMTEDMPLEISYVPSTYLTEITHVLQALSEVEQLLNAPDLCAKDFQDLFKQEESLKNIKDNLQQISGRIDVIHNKKAAALQSTTPPEKARLQEALSRLDFQWERVNKMYKDRQGQFDRSVEKWRRFHYDMKIFNQWLTEAEHFLKKTQIPENWEHAKYKWYLKELQDGIGQRQTVVRVLNATGEEIIQQSSKIDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDLNEFVLWLEEAGNISSIPLEPGNEQQLKEKLEEVKLLAEELPLRQGILKQLNETGGTVLVSAPISPEEQDKLENKLKQTNLQWIKVSRSLPEKQGEIEAHIKDLGQLEEQLNHLLLWLSPIRSQLEIYNQPNQTGPFDIKETEVAVQAKQPDVEGILSKGQNLYKEKPATEPVKRKLEDLSSEWKAVTHLLQELRAKWPGPVPGLTATGAPPSQTVALVTQPVVAKETAVSKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVLKAQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVVGQARAKLETWKEGPYTMDAIQRKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRVSERETALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKILRSLEGSDDAVLLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDIHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEEKAQNVTRLLRKQAEEVNTEWEKLNLHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVSHVSDLARQLTTLGIQLSPYNLSTLEDLNTRWKLLQVAVEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSSGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKEQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDSSTGLEEVMEQLNNSFPSSRGRNTPGKPMREDTM

Target ORF information:

RefSeq Version	XM_024988361.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophin (DMD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988361.1

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAATTTTCT AAGTTCGGGA AGCAGCACAT AGAAAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTC TTGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCAGGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAATA TCGGAAGCTC TGACATAGTA 
GATGGAAATC ATAAACTGAC TCTTGGCTTG ATTTGGAATA TAATCCTCCA TTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA GAAGATACTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT CAACTTCACC 
ACCAGCTGGT CTGACGGTTT GGCTTTGAAT GCGCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TCGCCAGCAG TCAGCTACAC AACGACTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTACTGG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TACGTCACAT CTCTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCCTTGAAGC CATCCAGGAG GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACAAGAGAAG AACATTTTCA ACTACATCAT CAAATGCACT ACTCTCAACA GATCACTGTC 
AGTCTAGCAC AGGGCTATGA ACGAACTCCT TCCTCTCCTC AGCCTCGCTT CAAGAGCTAT 
GCCTACACGC AGGCTGCTTA TGTCTCCACC TCTGACCCCA CACGGAGCCC ATTTCCTTCA 
CAGCGTTTGG AAGCTCCTGA AGACAAGTCA TTTGGCAGTC CATTGATGGA GACTGAAGTA 
AACCTGGACA GTTACCAGAC AGCTTTAGAA GAAGTACTCT CATGGCTCCT TTCAGCCGAA 
GACACATTGC AAGCACAAGG AGAGATTTCT AATGATGTAG AAGAAGTGAA AGAACAGTTT 
CATACCCACG AGGGATACAT GATGGATTTG ACATCCCATC AGGGACGGGT TGGCAATGTT 
CTCCAATTGG GGAGTCAGCT GATTGGGTCA GGGAAATTAT CAGAAGATGA AGAAACTGAA 
GTGCAAGAAC AAATGAATCT TCTAAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTC ATGGATCTCC AGAATCAGCA GCTGAAAGAG 
TTAAATGCCT GGTTAACAAA AACAGAAGAG AGAACAAGGA AAATGGAAAA AGAGCCCCTT 
GGACCTGATC TTGAGGACCT CAAACGCCAA ATACAGCAAC ATAAGGTGCT TCAAGAAGAC 
CTAGAACAGG AACAAGTCAG AGTCAATTCT CTCACGCACA TGGTGGTGGT TGTAGATGAG 
TCTAGTGGTG ATCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGG 
TGGGCAAACA TCTGTAGGTG GACTGAAGAT CGCTGGGTTC TTTTACAGGA TGTTCTTCTA 
AAATGGCAAC GTTTTACTGA AGAGCAGTGC CTTTTTAGTA CATGGCTTTC AGATAAAGAA 
GATGCATTGA ACAAGATTCC CACAAGTGGC TTTAAGGATC AAAGTGAAAT GTTATCAAGT 
CTTCAAAAAC TGGCCGTTTT AAAAACTGAT CTAGAAAAGA AAAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCATGA TCTCCTTTCA ACACTGAAAA ATACATTGGT TGCCCAAAAG 
ATGGAAGCAT GGTTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA AAAACTTGAA 
AAGAGTTCAA CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACGGTAAC TATGGTGACC ACGAGGGAGC AAATCCTGGT AAAGCATGCC 
CAAGAGGAAC TCCCTCCACC ACCTCCACAA AAGAAGAGGC AGATTATTGT GGATTCTGAA 
ATTAAGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCCGTGTTAC AGAGTCCTGA ATTTGCAGTC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGTATCAAAC AAGCCTCAGA ACAACTGAAC AGCCGGTGGA TCGAGTTCTG CCAATTGCTA 
AGTGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCA GAAAACTGGT TGAAAACTCA ACCCACTACC 
CCATCAGAGC CAACAGCAGT TAAAAGCCAG TTAAAAAATT GTAAGGATGA AGTCAACCGG 
CTGTCAGCTC TTCAGCCTCA AATCGAGAGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCC GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATATGCA GGCCAGAGAG AAAGAGTTAC AGACAATTTT GGACACTTTA 
CCCACCGTGC GGTATCAGGA GACCATGAGT ACCATCCTCA CGTGGATCCA ACAGTCAGAA 
ACCAAACTCT CGATACCTCA GGTTACTGTC ACTGAATACG AGATCATGGA GCAGAGACTT 
GAGGAGTTAC AGGCTTTACA AAGCTCTCTG CAAGAGCACC AAAATGACCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAGGAAA GCACCCTCTC ATATAAGCCA GAGATATCAG 
TCAGAATTTG AAAACATTGA GGGTCGGTGG AAGAAACTCT CTGCCCAGCT GAATGAACGT 
TGCCAAAAGC TAGAGGAGCA AATGGCCAAA CTCCGAAAAC TTCAGAATCA CATAAAAACA 
CTGAAGAAGT GGATGGCTGA AGTTGATGTT TTCTTGAAAG ATGAATGGCC TGCCCTTGGA 
GATTCAGAAA TTCTTAAAAA ACAGCTGAAG CAGTGCAGGC TTTTAGTCAG TGATATTCAG 
ACAATCCAGC CCAGTCTAAA CAGTGTCAAT GAAGGTGGAC AGAAAATAAA AACTGAAGCG 
GAGCCAGAGT TTGCTTCTAG ACTGGAGACA GAGCTCAGAG AACTTAACAC TCAATGGGAT 
TACATATGTC GACAGGTCTA TGCCAGAAAG GAGGCCTTAA AGGGAGGCTT GGATAAAACT 
GTAAGCCTTC AGAAAGATTT GTCAGAGATG CATGAATGGA TGACACAAGC CGAAGAAGAA 
TACCTAGAGA GAGATTTTGA ATATAAAACC CCAGATGAAT TACAGACAGC AGTTGAAGAG 
ATGAAGAGAG CTAAAGAAGA GGCCCAGCAA AAAGAAGCAA AAGTGCAGCT CCTCACTGAG 
TCCGTGAACA GTGTCATAGC TCAAGCTCCA CCTGCAGCAC AAGAGGCCTT AAGAAAGGAA 
CTGGACACTC TGACCACCAA CTACCAGTGG CTCTGCACCA GGCTGAATGG AAAATGCAAG 
ACGTTAGAAG AGGTTTGGGC ATGTTGGCAT GAGTTATTGT CATACTTGGA AAAGGCAAAT 
AAGTGGCTAA ATGAAGTAGA ATTGAAACTT AAGACTACTG AAAATATTCC TGGTGGAGCT 
GAGGAAATCT CTGAGGTGCT CAGTTCACTT GAAAATTTGA TGCAACATTC AGAAGATAAC 
CCGAATCAGA TTCGCATATT GGCACAGACC CTAACAGATG GTGGAGTCAT GGATGAACTG 
ATCAATGAAG AACTCGAGAC ATTTAATTCT CGTTGGAGAG AACTACATGA AGAGGCTGTG 
AGGAGGCAAA AGTTGCTTGA ACAGAGTATC CAGTCTGCCC AGGAGATTGA AAAATCCTTA 
CAGTTAATTC AGGAATCCCT CTCATCAATT GACAAGCAGT TGGCAGCTTA TATCGCTGAC 
AAAGTGGACG CCGCTCAGAT GCCTCAGGAA GCCCAGAAAA TCCAATCTGA TTTGACAAGT 
CATGAGATCA GTTTAGAAGA AATGAAGAAA CATTACCAAG GAAAGGAAAC TGCTCAACGG 
GTACTATCCC AAATTGAAGT GGCACAGAAA AAAATGCAAG ATGTTTCCAT GAAATTTCGA 
TTGTTCCAGA AGCCAGCCAA TTTTGAGCAG CGTCTACAGG AAAGTAAGAT GATTTTAGAT 
GAAGTGAAGA TGCACTTGCC CGCACTGGAA ACAAAGAGTG TTGAACAGGA AGTAGTACAG 
TCACAGTTAA ATCATTGTGT GAACTTGTAT AAAAGTCTGA GTGAAGTGAA GTCTGAAGTG 
GAAATGGTGA TTAAAACTGG ACGTCAAATT GTACAGAAGA AGCAGACGGA AAACCCTAAA 
GAGCTTGATG AAAGAGTAAC GGCTTTGAAA TTGCATTATA ATGAGCTGGG AGCAAAGGTA 
ACAGAAAGAA AGCAACAGTT GGAGAAATGC TTGAAATTGT CCCGTAAGAT GCGAAAGGAA 
ATGAATGCCT TGACAGAATG GCTGGCAGCT ACAGATTTGG AACTGACAAA GAGATCAGCA 
GTTGAAGGAA TGCCTAGTAA TTTGGATTCT GAAGTTGCCT GGGGAAAGGC TACTCAGAAA 
GAGACTGAGA AACAGAAGGT TCACCTAAAG AGCATTACAG AGCTAGGAGA GGCCTTGAAA 
ACGGTTTTGG GCAAGAAGGA GACCTTGGTC GAAGATAAAC TGAGTCTTTT GAATAGTAAC 
TGGATAGCCG TCACTTCTCG AGCAGAAGAG TGGCTAAACC TTTTGTTGGA ATACCAGAAA 
CACATGGAAA CCTTTGACCA GAATGTGGAT CATATCACAA AGTGGATCAT TCAAGCTGAT 
GCACTTCTGG ATGAATCTGA GAAAAAGAAA CCACAGCAAA AAGAAGACAT GCTAAAGCGC 
TTAAAGGCTG AACTGAATGA CATACGCCCA AAGGTGGACT CCACGCGTGA CCAAGCAGCA 
AACTTGATGG CAAACCGCGG CGACCACTGC AGGAAAGTAA TAGAGCCCAA AATCTCAGAG 
CTCAACCATC GATTTGCAGC CATTTCTCAC AGAATTAAGA CTGGAAAGGC CTCCATTCCT 
TTGAAGGAAT TGGAGCAGTT TAACTCAGAT ATACAAAAAT TGCTTGAACC ACTGGAGGCT 
GAAATTCAGC AGGGGGTGAA TCTGAAAGAG GAAGACTTCA ATAAAGATAT GAGTGAAGAC 
AATGAGGGTA CTGTAAAAGA ATTGTTGCAA AGAGGAGACA ACTTACAACA AAGAATCACA 
GATGAGAGAA AGCGAGAGGA AATAAAGATA AAACAGCAGC TGTTACAGAC AAAACATAAT 
GCTCTCAAGG ATTTGAGGTC TCAAAGAAGA AAAAAGGCTC TAGAAATTTC TCACCAGTGG 
TATCAGTACA AGAGGCAGGC TGATGATCTC CTGAAATGCT TGGATGACAT TGAAAAAAAA 
TTAGCCAGCC TACCTGAACC CAGAGATGAA AGGAAAATAA AGGAAATTGA TCGTGAGTTG 
CAGAAGAAGA AAGAGGAGCT GAATGCAGTG CATAGGCAAG CTGAGGGCTT GTCTGAGGAT 
GGGGCAGCAA TGGCAGTGGA GTCAACTCAG ATCCAGCTCA GCAAGCGCTG GCGGGAAATT 
GAGAGCAAAT TTGCTCAGTT TCGAAGACTC AACTTTGCAC AAATTCACAC TGTCCATGAA 
GAATCAGTGA TGGTGATGAC CGAAGACATG CCTTTGGAAA TTTCTTATGT GCCTTCTACT 
TACCTGACTG AGATCACTCA CGTCTTACAA GCCCTTTCAG AAGTGGAACA ACTTCTCAAT 
GCTCCCGACC TCTGTGCTAA AGATTTTCAA GATCTCTTCA AACAAGAGGA ATCTCTGAAG 
AATATAAAAG ACAACCTGCA ACAAATCTCA GGTCGGATTG ATGTTATTCA CAATAAGAAG 
GCGGCAGCAT TGCAAAGCAC CACACCCCCA GAAAAGGCAA GGTTACAGGA AGCTCTCTCC 
AGGCTCGACT TCCAATGGGA AAGAGTGAAC AAAATGTACA AGGACCGACA AGGGCAATTT 
GACAGATCTG TTGAAAAATG GCGACGGTTT CATTATGATA TGAAGATATT TAATCAATGG 
CTAACAGAAG CTGAACACTT TCTCAAAAAG ACACAAATTC CTGAGAACTG GGAACATGCC 
AAATATAAAT GGTATCTTAA GGAACTCCAG GATGGCATCG GGCAGCGGCA AACTGTTGTC 
AGAGTATTGA ATGCAACTGG GGAAGAAATA ATTCAGCAGT CCTCAAAAAT AGATGCCAGT 
ATTCTACAAG AAAAATTGGG AAGCCTGAAT CTGCGGTGGC AGGAGGTCTG CAAACAGCTG 
GCCGAGAGAA AAAAGAGGTT AGAAGAACAA AAGAATATCC TGTCAGAATT TCAAAGAGAT 
TTGAATGAAT TTGTTTTATG GTTGGAAGAA GCAGGTAACA TTTCTAGTAT TCCTCTTGAA 
CCTGGAAATG AGCAGCAACT AAAAGAAAAG CTTGAGGAAG TCAAGTTACT GGCCGAAGAG 
TTGCCCCTGC GTCAGGGAAT TCTAAAACAA TTAAATGAAA CAGGAGGAAC AGTGCTTGTA 
AGTGCTCCCA TAAGCCCAGA AGAGCAAGAT AAACTTGAAA ATAAGCTCAA GCAGACAAAT 
CTTCAGTGGA TAAAGGTTTC TAGGAGTTTA CCTGAGAAAC AGGGAGAAAT TGAGGCTCAC 
ATAAAAGACC TTGGACAGCT TGAAGAGCAA TTAAATCATC TGCTTCTGTG GCTGTCTCCT 
ATTAGGAGTC AGTTGGAAAT TTACAACCAA CCAAATCAGA CAGGACCATT TGACATTAAG 
GAAACTGAAG TAGCAGTTCA AGCTAAACAG CCGGATGTGG AAGGGATTTT GTCTAAAGGG 
CAGAATTTGT ACAAGGAAAA ACCAGCCACT GAGCCAGTGA AGAGGAAGTT AGAAGATCTG 
AGCTCGGAGT GGAAGGCGGT AACCCACTTA CTTCAAGAGC TGAGGGCAAA GTGGCCTGGC 
CCAGTCCCTG GACTGACAGC AACTGGAGCA CCTCCTAGTC AGACGGTTGC TCTGGTGACA 
CAACCTGTGG TTGCCAAGGA AACCGCTGTC TCCAAACTAG AAATGCCATC TTCCTTGCTG 
TTGGAGGTCC CTGCACTGGC AGATTTCAAC CGGGCTTGGA CAGAACTTAC CGACTGGCTG 
TCTCTGCTTG ATCGAGTTCT GAAAGCACAG AGGGTGATGG TGGGTGATCT TGAAGACATC 
AACGAGATGA TCATCAAGCA GAAGGCAACA CTGCAGGACT TGGAGCAGAG GCGCCCCCAG 
TTGGAAGAAC TCATTACTGC TGCCCAGAAT TTGAAAAACA AGACCAGCAA TCAAGAGGCC 
AGGACCATCA TTACTGATCG AATTGAAAGA ATTCAGAGTC AGTGGGATGA AGTACAGGAA 
CACCTTCAGA ACCGGAGGCA GCAGTTAAAT GAAATGTTAA AGGATTCAAC ACAATGGCTG 
GAAGCTAAGG AAGAAGCTGA GCAGGTGGTG GGGCAGGCCA GAGCCAAGCT TGAGACATGG 
AAGGAGGGTC CCTACACAAT GGATGCAATC CAAAGGAAGA TCACAGAAAC CAAGCAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTGG CAAATGACTT GGCACTGAAA 
CTTCTCCGGG ATTATTCTGC AGATGATACC CGAAAGGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT TCATAAAAGA GTGAGTGAGC GAGAGACTGC TCTGGAAGAA 
ACTCATAGAT TACTGCAACA GTTCCCCCTG GACTTGGAGA AGTTTCTTGC CTGGCTTACA 
GAAGCTGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GACTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAGG ACCTCCAAGG AGAAATTGAA 
GCTCACACAG ATATCTATCA CAACCTAGAT GAAAATGGCC AAAAAATCCT GAGATCCCTG 
GAAGGTTCTG ATGACGCGGT CCTATTACAA AGACGTTTGG ATAATATGAA TTTCAAGTGG 
AGTGAGCTGC GGAAAAAGTC TCTCAATATC AGGTCCCATT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTGT GGCTACAGCT GAAAGACGAT 
GAGTTGAGCC GTCAGGCACC TATTGGAGGC GATTTCCCAG CAGTTCAGAA GCAGAATGAT 
ATACACAGGG CCTTCAAGAG GGAACTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTGGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCGCC TGAGGAGAAA GCCCAGAATG TCACACGACT CCTACGAAAG 
CAGGCTGAGG AGGTCAACAC AGAGTGGGAA AAATTGAACC TACACTCGGC TGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAAGAACTGC AGGAGGCCAC GGACGAGCTG 
GACCTCAAAC TGCGCCAAGC TGAGGTGATC AAGGGATCAT GGCAGCCCGT GGGCGATCTT 
CTCATTGACT CCCTCCAGGA TCACCTCGAG AAAGTCAAGG CACTTCGAGG AGAAATTGCA 
CCTCTCAAAG AGAATGTAAG CCACGTCAGT GACCTTGCTC GCCAACTCAC CACGTTGGGC 
ATTCAGCTTT CACCATATAA TCTCAGCACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CTGTTGAGGA CCGCATCAGG CAGCTGCATG AAGCCCACAG GGACTTTGGC 
CCAGCGTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCT 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCGGCTTAT 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT TTTGAGCCTG 
TCAGCCGCAT GTGATGCCTT GGACCAGCAC AACCTCAAGC AAAACGACCA GCCCATGGAC 
ATCCTGCAGA TCATTAACTG TTTGACCACT ATTTATGACC GCCTGGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GATACGGGAC GAACAGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCGC ATTTGGAAGA CAAGTACAGA TACCTTTTCA AGCAAGTGGC CAGTTCAACA 
GGATTTTGTG ACCAGCGCAG ACTGGGCCTC CTTCTACATG ACTCTATCCA AATCCCAAGA 
CAGTTGGGTG AAGTTGCATC CTTTGGGGGC AGTAACATTG AGCCAAGTGT CAGGAGCTGC 
TTCCAATTCG CTAATAATAA GCCTGAGATC GAAGCAGCTC TCTTCCTCGA TTGGATGAGA 
CTAGAACCCC AGTCCATGGT GTGGCTGCCC GTCCTGCACA GGGTGGCTGC CGCGGAGACC 
GCCAAGCATC AGGCCAAGTG TAACATCTGC AAGGAGTGTC CAATCATCGG CTTCAGGTAC 
AGAAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGCT GCTTTTTCTC TGGCCGCGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATATT GTACTCCGAC TACATCGGGA 
GAAGATGTTC GAGACTTTGC CAAGGTACTA AAAAACAAAT TTCGCACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACGG TCTTAGAGGG GGACAACATG 
GAAACTCCTG TTACTCTGAT CAACTTCTGG CCGGTAGACT CTGCGCCTGC CTCGTCCCCT 
CAGCTTTCAC ACGATGATAC TCATTCACGC ATTGAACATT ATGCTAGCAG GCTAGCAGAA 
ATGGAAAACA GCAGTGGATC TTATCTAAAT GATAGCATCT CTCCTAATGA GAGCATAGAT 
GATGAACATT TGTTAATCCA GCATTACTGC CAAAGTTTGA ACCAGGACTC CCCCCTGAGC 
CAGCCTCGTA GTCCTGCCCA GATCTTGATT TCCTTAGAGA GTGAGGAAAG AGGGGAGCTA 
GAGAGAATCC TAGCAGATCT TGAGGAAGAA AACAGGAATC TGCAGGCTGA ATATGATCGT 
CTCAAGGAGC AGCATGAACA TAAAGGCCTG TCCCCACTGC CGTCCCCTCC TGAGATGATG 
CCCACCTCGC CACAGAGTCC CCGGGATGCT GAGCTCATTG CTGAGGCCAA GCTACTGCGT 
CAACACAAAG GCCGCCTGGA AGCCAGGATG CAAATCCTGG AAGACCACAA TAAACAGCTG 
GAGTCCCAGT TACACAGGCT CAGGCAGCTG CTGGAGCAAC CCCAGGCTGA GGCCAAGGTG 
AATGGCACAA CGGTGTCCTC TCCTTCTACC TCTCTTCAGA GGTCAGACAG CAGCCAGCCT 
ATGCTGCTCC GGGTGGTTGG CAGTCAAACT TCAGAATCCA TGGGCGAGGA AGACCTTCTC 
AGTCCTCCCC AGGACTCAAG CACAGGGTTA GAGGAAGTGA TGGAGCAACT CAACAACTCC 
TTCCCTAGTT CCAGAGGAAG AAATACCCCT GGGAAGCCAA TGAGAGAGGA CACAATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_024988362.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844130.1 Bos taurus dystrophin (DMD), transcript variant X5, mRNA.

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CloneID	OBa238541
Clone ID Related Accession (Same CDS sequence)	XM_024988362.1
Accession Version	XM_024988362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAATTTTCT AAGTTCGGGA AGCAGCACAT AGAAAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTC TTGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCAGGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAATA TCGGAAGCTC TGACATAGTA 
GATGGAAATC ATAAACTGAC TCTTGGCTTG ATTTGGAATA TAATCCTCCA TTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA GAAGATACTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT CAACTTCACC 
ACCAGCTGGT CTGACGGTTT GGCTTTGAAT GCGCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TCGCCAGCAG TCAGCTACAC AACGACTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTACTGG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TACGTCACAT CTCTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCCTTGAAGC CATCCAGGAG GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACAAGAGAAG AACATTTTCA ACTACATCAT CAAATGCACT ACTCTCAACA GATCACTGTC 
AGTCTAGCAC AGGGCTATGA ACGAACTCCT TCCTCTCCTC AGCCTCGCTT CAAGAGCTAT 
GCCTACACGC AGGCTGCTTA TGTCTCCACC TCTGACCCCA CACGGAGCCC ATTTCCTTCA 
CAGCGTTTGG AAGCTCCTGA AGACAAGTCA TTTGGCAGTC CATTGATGGA GACTGAAGTA 
AACCTGGACA GTTACCAGAC AGCTTTAGAA GAAGTACTCT CATGGCTCCT TTCAGCCGAA 
GACACATTGC AAGCACAAGG AGAGATTTCT AATGATGTAG AAGAAGTGAA AGAACAGTTT 
CATACCCACG AGGGATACAT GATGGATTTG ACATCCCATC AGGGACGGGT TGGCAATGTT 
CTCCAATTGG GGAGTCAGCT GATTGGGTCA GGGAAATTAT CAGAAGATGA AGAAACTGAA 
GTGCAAGAAC AAATGAATCT TCTAAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTC ATGGATCTCC AGAATCAGCA GCTGAAAGAG 
TTAAATGCCT GGTTAACAAA AACAGAAGAG AGAACAAGGA AAATGGAAAA AGAGCCCCTT 
GGACCTGATC TTGAGGACCT CAAACGCCAA ATACAGCAAC ATAAGGTGCT TCAAGAAGAC 
CTAGAACAGG AACAAGTCAG AGTCAATTCT CTCACGCACA TGGTGGTGGT TGTAGATGAG 
TCTAGTGGTG ATCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGG 
TGGGCAAACA TCTGTAGGTG GACTGAAGAT CGCTGGGTTC TTTTACAGGA TGTTCTTCTA 
AAATGGCAAC GTTTTACTGA AGAGCAGTGC CTTTTTAGTA CATGGCTTTC AGATAAAGAA 
GATGCATTGA ACAAGATTCC CACAAGTGGC TTTAAGGATC AAAGTGAAAT GTTATCAAGT 
CTTCAAAAAC TGGCCGTTTT AAAAACTGAT CTAGAAAAGA AAAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCATGA TCTCCTTTCA ACACTGAAAA ATACATTGGT TGCCCAAAAG 
ATGGAAGCAT GGTTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA AAAACTTGAA 
AAGAGTTCAA CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACGGTAAC TATGGTGACC ACGAGGGAGC AAATCCTGGT AAAGCATGCC 
CAAGAGGAAC TCCCTCCACC ACCTCCACAA AAGAAGAGGC AGATTATTGT GGATTCTGAA 
ATTAAGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCCGTGTTAC AGAGTCCTGA ATTTGCAGTC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGTATCAAAC AAGCCTCAGA ACAACTGAAC AGCCGGTGGA TCGAGTTCTG CCAATTGCTA 
AGTGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCA GAAAACTGGT TGAAAACTCA ACCCACTACC 
CCATCAGAGC CAACAGCAGT TAAAAGCCAG TTAAAAAATT GTAAGGATGA AGTCAACCGG 
CTGTCAGCTC TTCAGCCTCA AATCGAGAGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCC GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATATGCA GGCCAGAGAG AAAGAGTTAC AGACAATTTT GGACACTTTA 
CCCACCGTGC GGTATCAGGA GACCATGAGT ACCATCCTCA CGTGGATCCA ACAGTCAGAA 
ACCAAACTCT CGATACCTCA GGTTACTGTC ACTGAATACG AGATCATGGA GCAGAGACTT 
GAGGAGTTAC AGGCTTTACA AAGCTCTCTG CAAGAGCACC AAAATGACCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAGGAAA GCACCCTCTC ATATAAGCCA GAGATATCAG 
TCAGAATTTG AAAACATTGA GGGTCGGTGG AAGAAACTCT CTGCCCAGCT GAATGAACGT 
TGCCAAAAGC TAGAGGAGCA AATGGCCAAA CTCCGAAAAC TTCAGAATCA CATAAAAACA 
CTGAAGAAGT GGATGGCTGA AGTTGATGTT TTCTTGAAAG ATGAATGGCC TGCCCTTGGA 
GATTCAGAAA TTCTTAAAAA ACAGCTGAAG CAGTGCAGGC TTTTAGTCAG TGATATTCAG 
ACAATCCAGC CCAGTCTAAA CAGTGTCAAT GAAGGTGGAC AGAAAATAAA AACTGAAGCG 
GAGCCAGAGT TTGCTTCTAG ACTGGAGACA GAGCTCAGAG AACTTAACAC TCAATGGGAT 
TACATATGTC GACAGGTCTA TGCCAGAAAG GAGGCCTTAA AGGGAGGCTT GGATAAAACT 
GTAAGCCTTC AGAAAGATTT GTCAGAGATG CATGAATGGA TGACACAAGC CGAAGAAGAA 
TACCTAGAGA GAGATTTTGA ATATAAAACC CCAGATGAAT TACAGACAGC AGTTGAAGAG 
ATGAAGAGAG CTAAAGAAGA GGCCCAGCAA AAAGAAGCAA AAGTGCAGCT CCTCACTGAG 
TCCGTGAACA GTGTCATAGC TCAAGCTCCA CCTGCAGCAC AAGAGGCCTT AAGAAAGGAA 
CTGGACACTC TGACCACCAA CTACCAGTGG CTCTGCACCA GGCTGAATGG AAAATGCAAG 
ACGTTAGAAG AGGTTTGGGC ATGTTGGCAT GAGTTATTGT CATACTTGGA AAAGGCAAAT 
AAGTGGCTAA ATGAAGTAGA ATTGAAACTT AAGACTACTG AAAATATTCC TGGTGGAGCT 
GAGGAAATCT CTGAGGTGCT CAGTTCACTT GAAAATTTGA TGCAACATTC AGAAGATAAC 
CCGAATCAGA TTCGCATATT GGCACAGACC CTAACAGATG GTGGAGTCAT GGATGAACTG 
ATCAATGAAG AACTCGAGAC ATTTAATTCT CGTTGGAGAG AACTACATGA AGAGGCTGTG 
AGGAGGCAAA AGTTGCTTGA ACAGAGTATC CAGTCTGCCC AGGAGATTGA AAAATCCTTA 
CAGTTAATTC AGGAATCCCT CTCATCAATT GACAAGCAGT TGGCAGCTTA TATCGCTGAC 
AAAGTGGACG CCGCTCAGAT GCCTCAGGAA GCCCAGAAAA TCCAATCTGA TTTGACAAGT 
CATGAGATCA GTTTAGAAGA AATGAAGAAA CATTACCAAG GAAAGGAAAC TGCTCAACGG 
GTACTATCCC AAATTGAAGT GGCACAGAAA AAAATGCAAG ATGTTTCCAT GAAATTTCGA 
TTGTTCCAGA AGCCAGCCAA TTTTGAGCAG CGTCTACAGG AAAGTAAGAT GATTTTAGAT 
GAAGTGAAGA TGCACTTGCC CGCACTGGAA ACAAAGAGTG TTGAACAGGA AGTAGTACAG 
TCACAGTTAA ATCATTGTGT GAACTTGTAT AAAAGTCTGA GTGAAGTGAA GTCTGAAGTG 
GAAATGGTGA TTAAAACTGG ACGTCAAATT GTACAGAAGA AGCAGACGGA AAACCCTAAA 
GAGCTTGATG AAAGAGTAAC GGCTTTGAAA TTGCATTATA ATGAGCTGGG AGCAAAGGTA 
ACAGAAAGAA AGCAACAGTT GGAGAAATGC TTGAAATTGT CCCGTAAGAT GCGAAAGGAA 
ATGAATGCCT TGACAGAATG GCTGGCAGCT ACAGATTTGG AACTGACAAA GAGATCAGCA 
GTTGAAGGAA TGCCTAGTAA TTTGGATTCT GAAGTTGCCT GGGGAAAGGC TACTCAGAAA 
GAGACTGAGA AACAGAAGGT TCACCTAAAG AGCATTACAG AGCTAGGAGA GGCCTTGAAA 
ACGGTTTTGG GCAAGAAGGA GACCTTGGTC GAAGATAAAC TGAGTCTTTT GAATAGTAAC 
TGGATAGCCG TCACTTCTCG AGCAGAAGAG TGGCTAAACC TTTTGTTGGA ATACCAGAAA 
CACATGGAAA CCTTTGACCA GAATGTGGAT CATATCACAA AGTGGATCAT TCAAGCTGAT 
GCACTTCTGG ATGAATCTGA GAAAAAGAAA CCACAGCAAA AAGAAGACAT GCTAAAGCGC 
TTAAAGGCTG AACTGAATGA CATACGCCCA AAGGTGGACT CCACGCGTGA CCAAGCAGCA 
AACTTGATGG CAAACCGCGG CGACCACTGC AGGAAAGTAA TAGAGCCCAA AATCTCAGAG 
CTCAACCATC GATTTGCAGC CATTTCTCAC AGAATTAAGA CTGGAAAGGC CTCCATTCCT 
TTGAAGGAAT TGGAGCAGTT TAACTCAGAT ATACAAAAAT TGCTTGAACC ACTGGAGGCT 
GAAATTCAGC AGGGGGTGAA TCTGAAAGAG GAAGACTTCA ATAAAGATAT GAGTGAAGAC 
AATGAGGGTA CTGTAAAAGA ATTGTTGCAA AGAGGAGACA ACTTACAACA AAGAATCACA 
GATGAGAGAA AGCGAGAGGA AATAAAGATA AAACAGCAGC TGTTACAGAC AAAACATAAT 
GCTCTCAAGG ATTTGAGGTC TCAAAGAAGA AAAAAGGCTC TAGAAATTTC TCACCAGTGG 
TATCAGTACA AGAGGCAGGC TGATGATCTC CTGAAATGCT TGGATGACAT TGAAAAAAAA 
TTAGCCAGCC TACCTGAACC CAGAGATGAA AGGAAAATAA AGGAAATTGA TCGTGAGTTG 
CAGAAGAAGA AAGAGGAGCT GAATGCAGTG CATAGGCAAG CTGAGGGCTT GTCTGAGGAT 
GGGGCAGCAA TGGCAGTGGA GTCAACTCAG ATCCAGCTCA GCAAGCGCTG GCGGGAAATT 
GAGAGCAAAT TTGCTCAGTT TCGAAGACTC AACTTTGCAC AAATTCACAC TGTCCATGAA 
GAATCAGTGA TGGTGATGAC CGAAGACATG CCTTTGGAAA TTTCTTATGT GCCTTCTACT 
TACCTGACTG AGATCACTCA CGTCTTACAA GCCCTTTCAG AAGTGGAACA ACTTCTCAAT 
GCTCCCGACC TCTGTGCTAA AGATTTTCAA GATCTCTTCA AACAAGAGGA ATCTCTGAAG 
AATATAAAAG ACAACCTGCA ACAAATCTCA GGTCGGATTG ATGTTATTCA CAATAAGAAG 
GCGGCAGCAT TGCAAAGCAC CACACCCCCA GAAAAGGCAA GGTTACAGGA AGCTCTCTCC 
AGGCTCGACT TCCAATGGGA AAGAGTGAAC AAAATGTACA AGGACCGACA AGGGCAATTT 
GACAGATCTG TTGAAAAATG GCGACGGTTT CATTATGATA TGAAGATATT TAATCAATGG 
CTAACAGAAG CTGAACACTT TCTCAAAAAG ACACAAATTC CTGAGAACTG GGAACATGCC 
AAATATAAAT GGTATCTTAA GGAACTCCAG GATGGCATCG GGCAGCGGCA AACTGTTGTC 
AGAGTATTGA ATGCAACTGG GGAAGAAATA ATTCAGCAGT CCTCAAAAAT AGATGCCAGT 
ATTCTACAAG AAAAATTGGG AAGCCTGAAT CTGCGGTGGC AGGAGGTCTG CAAACAGCTG 
GCCGAGAGAA AAAAGAGGTT AGAAGAACAA AAGAATATCC TGTCAGAATT TCAAAGAGAT 
TTGAATGAAT TTGTTTTATG GTTGGAAGAA GCAGGTAACA TTTCTAGTAT TCCTCTTGAA 
CCTGGAAATG AGCAGCAACT AAAAGAAAAG CTTGAGGAAG TCAAGTTACT GGCCGAAGAG 
TTGCCCCTGC GTCAGGGAAT TCTAAAACAA TTAAATGAAA CAGGAGGAAC AGTGCTTGTA 
AGTGCTCCCA TAAGCCCAGA AGAGCAAGAT AAACTTGAAA ATAAGCTCAA GCAGACAAAT 
CTTCAGTGGA TAAAGGTTTC TAGGAGTTTA CCTGAGAAAC AGGGAGAAAT TGAGGCTCAC 
ATAAAAGACC TTGGACAGCT TGAAGAGCAA TTAAATCATC TGCTTCTGTG GCTGTCTCCT 
ATTAGGAGTC AGTTGGAAAT TTACAACCAA CCAAATCAGA CAGGACCATT TGACATTAAG 
GAAACTGAAG TAGCAGTTCA AGCTAAACAG CCGGATGTGG AAGGGATTTT GTCTAAAGGG 
CAGAATTTGT ACAAGGAAAA ACCAGCCACT GAGCCAGTGA AGAGGAAGTT AGAAGATCTG 
AGCTCGGAGT GGAAGGCGGT AACCCACTTA CTTCAAGAGC TGAGGGCAAA GTGGCCTGGC 
CCAGTCCCTG GACTGACAGC AACTGGAGCA CCTCCTAGTC AGACGGTTGC TCTGGTGACA 
CAACCTGTGG TTGCCAAGGA AACCGCTGTC TCCAAACTAG AAATGCCATC TTCCTTGCTG 
TTGGAGGTCC CTGCACTGGC AGATTTCAAC CGGGCTTGGA CAGAACTTAC CGACTGGCTG 
TCTCTGCTTG ATCGAGTTCT GAAAGCACAG AGGGTGATGG TGGGTGATCT TGAAGACATC 
AACGAGATGA TCATCAAGCA GAAGGCAACA CTGCAGGACT TGGAGCAGAG GCGCCCCCAG 
TTGGAAGAAC TCATTACTGC TGCCCAGAAT TTGAAAAACA AGACCAGCAA TCAAGAGGCC 
AGGACCATCA TTACTGATCG AATTGAAAGA ATTCAGAGTC AGTGGGATGA AGTACAGGAA 
CACCTTCAGA ACCGGAGGCA GCAGTTAAAT GAAATGTTAA AGGATTCAAC ACAATGGCTG 
GAAGCTAAGG AAGAAGCTGA GCAGGTGGTG GGGCAGGCCA GAGCCAAGCT TGAGACATGG 
AAGGAGGGTC CCTACACAAT GGATGCAATC CAAAGGAAGA TCACAGAAAC CAAGCAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTGG CAAATGACTT GGCACTGAAA 
CTTCTCCGGG ATTATTCTGC AGATGATACC CGAAAGGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT TCATAAAAGA GTGAGTGAGC GAGAGACTGC TCTGGAAGAA 
ACTCATAGAT TACTGCAACA GTTCCCCCTG GACTTGGAGA AGTTTCTTGC CTGGCTTACA 
GAAGCTGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GACTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAGG ACCTCCAAGG AGAAATTGAA 
GCTCACACAG ATATCTATCA CAACCTAGAT GAAAATGGCC AAAAAATCCT GAGATCCCTG 
GAAGGTTCTG ATGACGCGGT CCTATTACAA AGACGTTTGG ATAATATGAA TTTCAAGTGG 
AGTGAGCTGC GGAAAAAGTC TCTCAATATC AGGTCCCATT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTGT GGCTACAGCT GAAAGACGAT 
GAGTTGAGCC GTCAGGCACC TATTGGAGGC GATTTCCCAG CAGTTCAGAA GCAGAATGAT 
ATACACAGGG CCTTCAAGAG GGAACTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTGGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCGCC TGAGGAGAAA GCCCAGAATG TCACACGACT CCTACGAAAG 
CAGGCTGAGG AGGTCAACAC AGAGTGGGAA AAATTGAACC TACACTCGGC TGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAAGAACTGC AGGAGGCCAC GGACGAGCTG 
GACCTCAAAC TGCGCCAAGC TGAGGTGATC AAGGGATCAT GGCAGCCCGT GGGCGATCTT 
CTCATTGACT CCCTCCAGGA TCACCTCGAG AAAGTCAAGG CACTTCGAGG AGAAATTGCA 
CCTCTCAAAG AGAATGTAAG CCACGTCAGT GACCTTGCTC GCCAACTCAC CACGTTGGGC 
ATTCAGCTTT CACCATATAA TCTCAGCACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CTGTTGAGGA CCGCATCAGG CAGCTGCATG AAGCCCACAG GGACTTTGGC 
CCAGCGTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCT 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCGGCTTAT 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT TTTGAGCCTG 
TCAGCCGCAT GTGATGCCTT GGACCAGCAC AACCTCAAGC AAAACGACCA GCCCATGGAC 
ATCCTGCAGA TCATTAACTG TTTGACCACT ATTTATGACC GCCTGGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GATACGGGAC GAACAGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCGC ATTTGGAAGA CAAGTACAGA TACCTTTTCA AGCAAGTGGC CAGTTCAACA 
GGATTTTGTG ACCAGCGCAG ACTGGGCCTC CTTCTACATG ACTCTATCCA AATCCCAAGA 
CAGTTGGGTG AAGTTGCATC CTTTGGGGGC AGTAACATTG AGCCAAGTGT CAGGAGCTGC 
TTCCAATTCG CTAATAATAA GCCTGAGATC GAAGCAGCTC TCTTCCTCGA TTGGATGAGA 
CTAGAACCCC AGTCCATGGT GTGGCTGCCC GTCCTGCACA GGGTGGCTGC CGCGGAGACC 
GCCAAGCATC AGGCCAAGTG TAACATCTGC AAGGAGTGTC CAATCATCGG CTTCAGGTAC 
AGAAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGCT GCTTTTTCTC TGGCCGCGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATATT GTACTCCGAC TACATCGGGA 
GAAGATGTTC GAGACTTTGC CAAGGTACTA AAAAACAAAT TTCGCACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACGG TCTTAGAGGG GGACAACATG 
GAAACGCTAG CAGAAATGGA AAACAGCAGT GGATCTTATC TAAATGATAG CATCTCTCCT 
AATGAGAGCA TAGATGATGA ACATTTGTTA ATCCAGCATT ACTGCCAAAG TTTGAACCAG 
GACTCCCCCC TGAGCCAGCC TCGTAGTCCT GCCCAGATCT TGATTTCCTT AGAGAGTGAG 
GAAAGAGGGG AGCTAGAGAG AATCCTAGCA GATCTTGAGG AAGAAAACAG GAATCTGCAG 
GCTGAATATG ATCGTCTCAA GGAGCAGCAT GAACATAAAG GCCTGTCCCC ACTGCCGTCC 
CCTCCTGAGA TGATGCCCAC CTCGCCACAG AGTCCCCGGG ATGCTGAGCT CATTGCTGAG 
GCCAAGCTAC TGCGTCAACA CAAAGGCCGC CTGGAAGCCA GGATGCAAAT CCTGGAAGAC 
CACAATAAAC AGCTGGAGTC CCAGTTACAC AGGCTCAGGC AGCTGCTGGA GCAACCCCAG 
GCTGAGGCCA AGGTGAATGG CACAACGGTG TCCTCTCCTT CTACCTCTCT TCAGAGGTCA 
GACAGCAGCC AGCCTATGCT GCTCCGGGTG GTTGGCAGTC AAACTTCAGA ATCCATGGGC 
GAGGAAGACC TTCTCAGTCC TCCCCAGGAC TCAAGCACAG GGTTAGAGGA AGTGATGGAG 
CAACTCAACA ACTCCTTCCC TAGTTCCAGA GGACACAATG TAGGAAGCCT TTTCCACTTG 
GCAGATGATT TGGGCAGAGC GATGGAGTCC TTAGTTTCAG TTATGACAGA TGAAGAAGGG 
GCAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844130.1
CDS	252..11240
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALQVLQKNNVDLVNIGSSDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVRQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYVTSLFQVLPQQVSLEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERTPSSPQPRFKSYAYTQAAYVSTSDPTRSPFPSQRLEAPEDKSFGSPLMETEVNLDSYQTALEEVLSWLLSAEDTLQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGSGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNAWLTKTEERTRKMEKEPLGPDLEDLKRQIQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDVLLKWQRFTEEQCLFSTWLSDKEDALNKIPTSGFKDQSEMLSSLQKLAVLKTDLEKKKQTMDKLCSLNHDLLSTLKNTLVAQKMEAWLDNFAQRWDNLVQKLEKSSTQISQAVTTTQPSLTQTTVMETVTMVTTREQILVKHAQEELPPPPPQKKRQIIVDSEIKKRLDVDITELHSWITRSEAVLQSPEFAVYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTPSEPTAVKSQLKNCKDEVNRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADMQAREKELQTILDTLPTVRYQETMSTILTWIQQSETKLSIPQVTVTEYEIMEQRLEELQALQSSLQEHQNDLNYLSTTVKEMSRKAPSHISQRYQSEFENIEGRWKKLSAQLNERCQKLEEQMAKLRKLQNHIKTLKKWMAEVDVFLKDEWPALGDSEILKKQLKQCRLLVSDIQTIQPSLNSVNEGGQKIKTEAEPEFASRLETELRELNTQWDYICRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVQLLTESVNSVIAQAPPAAQEALRKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLNEVELKLKTTENIPGGAEEISEVLSSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLQLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHYQGKETAQRVLSQIEVAQKKMQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDLELTKRSAVEGMPSNLDSEVAWGKATQKETEKQKVHLKSITELGEALKTVLGKKETLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMETFDQNVDHITKWIIQADALLDESEKKKPQQKEDMLKRLKAELNDIRPKVDSTRDQAANLMANRGDHCRKVIEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVHRQAEGLSEDGAAMAVESTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVMVMTEDMPLEISYVPSTYLTEITHVLQALSEVEQLLNAPDLCAKDFQDLFKQEESLKNIKDNLQQISGRIDVIHNKKAAALQSTTPPEKARLQEALSRLDFQWERVNKMYKDRQGQFDRSVEKWRRFHYDMKIFNQWLTEAEHFLKKTQIPENWEHAKYKWYLKELQDGIGQRQTVVRVLNATGEEIIQQSSKIDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDLNEFVLWLEEAGNISSIPLEPGNEQQLKEKLEEVKLLAEELPLRQGILKQLNETGGTVLVSAPISPEEQDKLENKLKQTNLQWIKVSRSLPEKQGEIEAHIKDLGQLEEQLNHLLLWLSPIRSQLEIYNQPNQTGPFDIKETEVAVQAKQPDVEGILSKGQNLYKEKPATEPVKRKLEDLSSEWKAVTHLLQELRAKWPGPVPGLTATGAPPSQTVALVTQPVVAKETAVSKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVLKAQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVVGQARAKLETWKEGPYTMDAIQRKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRVSERETALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKILRSLEGSDDAVLLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDIHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEEKAQNVTRLLRKQAEEVNTEWEKLNLHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVSHVSDLARQLTTLGIQLSPYNLSTLEDLNTRWKLLQVAVEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETLAEMENSSGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKEQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDSSTGLEEVMEQLNNSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_024988362.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophin (DMD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988362.1

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAATTTTCT AAGTTCGGGA AGCAGCACAT AGAAAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTC TTGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCAGGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAATA TCGGAAGCTC TGACATAGTA 
GATGGAAATC ATAAACTGAC TCTTGGCTTG ATTTGGAATA TAATCCTCCA TTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA GAAGATACTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT CAACTTCACC 
ACCAGCTGGT CTGACGGTTT GGCTTTGAAT GCGCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TCGCCAGCAG TCAGCTACAC AACGACTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTACTGG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TACGTCACAT CTCTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCCTTGAAGC CATCCAGGAG GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACAAGAGAAG AACATTTTCA ACTACATCAT CAAATGCACT ACTCTCAACA GATCACTGTC 
AGTCTAGCAC AGGGCTATGA ACGAACTCCT TCCTCTCCTC AGCCTCGCTT CAAGAGCTAT 
GCCTACACGC AGGCTGCTTA TGTCTCCACC TCTGACCCCA CACGGAGCCC ATTTCCTTCA 
CAGCGTTTGG AAGCTCCTGA AGACAAGTCA TTTGGCAGTC CATTGATGGA GACTGAAGTA 
AACCTGGACA GTTACCAGAC AGCTTTAGAA GAAGTACTCT CATGGCTCCT TTCAGCCGAA 
GACACATTGC AAGCACAAGG AGAGATTTCT AATGATGTAG AAGAAGTGAA AGAACAGTTT 
CATACCCACG AGGGATACAT GATGGATTTG ACATCCCATC AGGGACGGGT TGGCAATGTT 
CTCCAATTGG GGAGTCAGCT GATTGGGTCA GGGAAATTAT CAGAAGATGA AGAAACTGAA 
GTGCAAGAAC AAATGAATCT TCTAAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTC ATGGATCTCC AGAATCAGCA GCTGAAAGAG 
TTAAATGCCT GGTTAACAAA AACAGAAGAG AGAACAAGGA AAATGGAAAA AGAGCCCCTT 
GGACCTGATC TTGAGGACCT CAAACGCCAA ATACAGCAAC ATAAGGTGCT TCAAGAAGAC 
CTAGAACAGG AACAAGTCAG AGTCAATTCT CTCACGCACA TGGTGGTGGT TGTAGATGAG 
TCTAGTGGTG ATCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGG 
TGGGCAAACA TCTGTAGGTG GACTGAAGAT CGCTGGGTTC TTTTACAGGA TGTTCTTCTA 
AAATGGCAAC GTTTTACTGA AGAGCAGTGC CTTTTTAGTA CATGGCTTTC AGATAAAGAA 
GATGCATTGA ACAAGATTCC CACAAGTGGC TTTAAGGATC AAAGTGAAAT GTTATCAAGT 
CTTCAAAAAC TGGCCGTTTT AAAAACTGAT CTAGAAAAGA AAAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCATGA TCTCCTTTCA ACACTGAAAA ATACATTGGT TGCCCAAAAG 
ATGGAAGCAT GGTTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA AAAACTTGAA 
AAGAGTTCAA CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACGGTAAC TATGGTGACC ACGAGGGAGC AAATCCTGGT AAAGCATGCC 
CAAGAGGAAC TCCCTCCACC ACCTCCACAA AAGAAGAGGC AGATTATTGT GGATTCTGAA 
ATTAAGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCCGTGTTAC AGAGTCCTGA ATTTGCAGTC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGTATCAAAC AAGCCTCAGA ACAACTGAAC AGCCGGTGGA TCGAGTTCTG CCAATTGCTA 
AGTGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCA GAAAACTGGT TGAAAACTCA ACCCACTACC 
CCATCAGAGC CAACAGCAGT TAAAAGCCAG TTAAAAAATT GTAAGGATGA AGTCAACCGG 
CTGTCAGCTC TTCAGCCTCA AATCGAGAGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCC GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATATGCA GGCCAGAGAG AAAGAGTTAC AGACAATTTT GGACACTTTA 
CCCACCGTGC GGTATCAGGA GACCATGAGT ACCATCCTCA CGTGGATCCA ACAGTCAGAA 
ACCAAACTCT CGATACCTCA GGTTACTGTC ACTGAATACG AGATCATGGA GCAGAGACTT 
GAGGAGTTAC AGGCTTTACA AAGCTCTCTG CAAGAGCACC AAAATGACCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAGGAAA GCACCCTCTC ATATAAGCCA GAGATATCAG 
TCAGAATTTG AAAACATTGA GGGTCGGTGG AAGAAACTCT CTGCCCAGCT GAATGAACGT 
TGCCAAAAGC TAGAGGAGCA AATGGCCAAA CTCCGAAAAC TTCAGAATCA CATAAAAACA 
CTGAAGAAGT GGATGGCTGA AGTTGATGTT TTCTTGAAAG ATGAATGGCC TGCCCTTGGA 
GATTCAGAAA TTCTTAAAAA ACAGCTGAAG CAGTGCAGGC TTTTAGTCAG TGATATTCAG 
ACAATCCAGC CCAGTCTAAA CAGTGTCAAT GAAGGTGGAC AGAAAATAAA AACTGAAGCG 
GAGCCAGAGT TTGCTTCTAG ACTGGAGACA GAGCTCAGAG AACTTAACAC TCAATGGGAT 
TACATATGTC GACAGGTCTA TGCCAGAAAG GAGGCCTTAA AGGGAGGCTT GGATAAAACT 
GTAAGCCTTC AGAAAGATTT GTCAGAGATG CATGAATGGA TGACACAAGC CGAAGAAGAA 
TACCTAGAGA GAGATTTTGA ATATAAAACC CCAGATGAAT TACAGACAGC AGTTGAAGAG 
ATGAAGAGAG CTAAAGAAGA GGCCCAGCAA AAAGAAGCAA AAGTGCAGCT CCTCACTGAG 
TCCGTGAACA GTGTCATAGC TCAAGCTCCA CCTGCAGCAC AAGAGGCCTT AAGAAAGGAA 
CTGGACACTC TGACCACCAA CTACCAGTGG CTCTGCACCA GGCTGAATGG AAAATGCAAG 
ACGTTAGAAG AGGTTTGGGC ATGTTGGCAT GAGTTATTGT CATACTTGGA AAAGGCAAAT 
AAGTGGCTAA ATGAAGTAGA ATTGAAACTT AAGACTACTG AAAATATTCC TGGTGGAGCT 
GAGGAAATCT CTGAGGTGCT CAGTTCACTT GAAAATTTGA TGCAACATTC AGAAGATAAC 
CCGAATCAGA TTCGCATATT GGCACAGACC CTAACAGATG GTGGAGTCAT GGATGAACTG 
ATCAATGAAG AACTCGAGAC ATTTAATTCT CGTTGGAGAG AACTACATGA AGAGGCTGTG 
AGGAGGCAAA AGTTGCTTGA ACAGAGTATC CAGTCTGCCC AGGAGATTGA AAAATCCTTA 
CAGTTAATTC AGGAATCCCT CTCATCAATT GACAAGCAGT TGGCAGCTTA TATCGCTGAC 
AAAGTGGACG CCGCTCAGAT GCCTCAGGAA GCCCAGAAAA TCCAATCTGA TTTGACAAGT 
CATGAGATCA GTTTAGAAGA AATGAAGAAA CATTACCAAG GAAAGGAAAC TGCTCAACGG 
GTACTATCCC AAATTGAAGT GGCACAGAAA AAAATGCAAG ATGTTTCCAT GAAATTTCGA 
TTGTTCCAGA AGCCAGCCAA TTTTGAGCAG CGTCTACAGG AAAGTAAGAT GATTTTAGAT 
GAAGTGAAGA TGCACTTGCC CGCACTGGAA ACAAAGAGTG TTGAACAGGA AGTAGTACAG 
TCACAGTTAA ATCATTGTGT GAACTTGTAT AAAAGTCTGA GTGAAGTGAA GTCTGAAGTG 
GAAATGGTGA TTAAAACTGG ACGTCAAATT GTACAGAAGA AGCAGACGGA AAACCCTAAA 
GAGCTTGATG AAAGAGTAAC GGCTTTGAAA TTGCATTATA ATGAGCTGGG AGCAAAGGTA 
ACAGAAAGAA AGCAACAGTT GGAGAAATGC TTGAAATTGT CCCGTAAGAT GCGAAAGGAA 
ATGAATGCCT TGACAGAATG GCTGGCAGCT ACAGATTTGG AACTGACAAA GAGATCAGCA 
GTTGAAGGAA TGCCTAGTAA TTTGGATTCT GAAGTTGCCT GGGGAAAGGC TACTCAGAAA 
GAGACTGAGA AACAGAAGGT TCACCTAAAG AGCATTACAG AGCTAGGAGA GGCCTTGAAA 
ACGGTTTTGG GCAAGAAGGA GACCTTGGTC GAAGATAAAC TGAGTCTTTT GAATAGTAAC 
TGGATAGCCG TCACTTCTCG AGCAGAAGAG TGGCTAAACC TTTTGTTGGA ATACCAGAAA 
CACATGGAAA CCTTTGACCA GAATGTGGAT CATATCACAA AGTGGATCAT TCAAGCTGAT 
GCACTTCTGG ATGAATCTGA GAAAAAGAAA CCACAGCAAA AAGAAGACAT GCTAAAGCGC 
TTAAAGGCTG AACTGAATGA CATACGCCCA AAGGTGGACT CCACGCGTGA CCAAGCAGCA 
AACTTGATGG CAAACCGCGG CGACCACTGC AGGAAAGTAA TAGAGCCCAA AATCTCAGAG 
CTCAACCATC GATTTGCAGC CATTTCTCAC AGAATTAAGA CTGGAAAGGC CTCCATTCCT 
TTGAAGGAAT TGGAGCAGTT TAACTCAGAT ATACAAAAAT TGCTTGAACC ACTGGAGGCT 
GAAATTCAGC AGGGGGTGAA TCTGAAAGAG GAAGACTTCA ATAAAGATAT GAGTGAAGAC 
AATGAGGGTA CTGTAAAAGA ATTGTTGCAA AGAGGAGACA ACTTACAACA AAGAATCACA 
GATGAGAGAA AGCGAGAGGA AATAAAGATA AAACAGCAGC TGTTACAGAC AAAACATAAT 
GCTCTCAAGG ATTTGAGGTC TCAAAGAAGA AAAAAGGCTC TAGAAATTTC TCACCAGTGG 
TATCAGTACA AGAGGCAGGC TGATGATCTC CTGAAATGCT TGGATGACAT TGAAAAAAAA 
TTAGCCAGCC TACCTGAACC CAGAGATGAA AGGAAAATAA AGGAAATTGA TCGTGAGTTG 
CAGAAGAAGA AAGAGGAGCT GAATGCAGTG CATAGGCAAG CTGAGGGCTT GTCTGAGGAT 
GGGGCAGCAA TGGCAGTGGA GTCAACTCAG ATCCAGCTCA GCAAGCGCTG GCGGGAAATT 
GAGAGCAAAT TTGCTCAGTT TCGAAGACTC AACTTTGCAC AAATTCACAC TGTCCATGAA 
GAATCAGTGA TGGTGATGAC CGAAGACATG CCTTTGGAAA TTTCTTATGT GCCTTCTACT 
TACCTGACTG AGATCACTCA CGTCTTACAA GCCCTTTCAG AAGTGGAACA ACTTCTCAAT 
GCTCCCGACC TCTGTGCTAA AGATTTTCAA GATCTCTTCA AACAAGAGGA ATCTCTGAAG 
AATATAAAAG ACAACCTGCA ACAAATCTCA GGTCGGATTG ATGTTATTCA CAATAAGAAG 
GCGGCAGCAT TGCAAAGCAC CACACCCCCA GAAAAGGCAA GGTTACAGGA AGCTCTCTCC 
AGGCTCGACT TCCAATGGGA AAGAGTGAAC AAAATGTACA AGGACCGACA AGGGCAATTT 
GACAGATCTG TTGAAAAATG GCGACGGTTT CATTATGATA TGAAGATATT TAATCAATGG 
CTAACAGAAG CTGAACACTT TCTCAAAAAG ACACAAATTC CTGAGAACTG GGAACATGCC 
AAATATAAAT GGTATCTTAA GGAACTCCAG GATGGCATCG GGCAGCGGCA AACTGTTGTC 
AGAGTATTGA ATGCAACTGG GGAAGAAATA ATTCAGCAGT CCTCAAAAAT AGATGCCAGT 
ATTCTACAAG AAAAATTGGG AAGCCTGAAT CTGCGGTGGC AGGAGGTCTG CAAACAGCTG 
GCCGAGAGAA AAAAGAGGTT AGAAGAACAA AAGAATATCC TGTCAGAATT TCAAAGAGAT 
TTGAATGAAT TTGTTTTATG GTTGGAAGAA GCAGGTAACA TTTCTAGTAT TCCTCTTGAA 
CCTGGAAATG AGCAGCAACT AAAAGAAAAG CTTGAGGAAG TCAAGTTACT GGCCGAAGAG 
TTGCCCCTGC GTCAGGGAAT TCTAAAACAA TTAAATGAAA CAGGAGGAAC AGTGCTTGTA 
AGTGCTCCCA TAAGCCCAGA AGAGCAAGAT AAACTTGAAA ATAAGCTCAA GCAGACAAAT 
CTTCAGTGGA TAAAGGTTTC TAGGAGTTTA CCTGAGAAAC AGGGAGAAAT TGAGGCTCAC 
ATAAAAGACC TTGGACAGCT TGAAGAGCAA TTAAATCATC TGCTTCTGTG GCTGTCTCCT 
ATTAGGAGTC AGTTGGAAAT TTACAACCAA CCAAATCAGA CAGGACCATT TGACATTAAG 
GAAACTGAAG TAGCAGTTCA AGCTAAACAG CCGGATGTGG AAGGGATTTT GTCTAAAGGG 
CAGAATTTGT ACAAGGAAAA ACCAGCCACT GAGCCAGTGA AGAGGAAGTT AGAAGATCTG 
AGCTCGGAGT GGAAGGCGGT AACCCACTTA CTTCAAGAGC TGAGGGCAAA GTGGCCTGGC 
CCAGTCCCTG GACTGACAGC AACTGGAGCA CCTCCTAGTC AGACGGTTGC TCTGGTGACA 
CAACCTGTGG TTGCCAAGGA AACCGCTGTC TCCAAACTAG AAATGCCATC TTCCTTGCTG 
TTGGAGGTCC CTGCACTGGC AGATTTCAAC CGGGCTTGGA CAGAACTTAC CGACTGGCTG 
TCTCTGCTTG ATCGAGTTCT GAAAGCACAG AGGGTGATGG TGGGTGATCT TGAAGACATC 
AACGAGATGA TCATCAAGCA GAAGGCAACA CTGCAGGACT TGGAGCAGAG GCGCCCCCAG 
TTGGAAGAAC TCATTACTGC TGCCCAGAAT TTGAAAAACA AGACCAGCAA TCAAGAGGCC 
AGGACCATCA TTACTGATCG AATTGAAAGA ATTCAGAGTC AGTGGGATGA AGTACAGGAA 
CACCTTCAGA ACCGGAGGCA GCAGTTAAAT GAAATGTTAA AGGATTCAAC ACAATGGCTG 
GAAGCTAAGG AAGAAGCTGA GCAGGTGGTG GGGCAGGCCA GAGCCAAGCT TGAGACATGG 
AAGGAGGGTC CCTACACAAT GGATGCAATC CAAAGGAAGA TCACAGAAAC CAAGCAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTGG CAAATGACTT GGCACTGAAA 
CTTCTCCGGG ATTATTCTGC AGATGATACC CGAAAGGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT TCATAAAAGA GTGAGTGAGC GAGAGACTGC TCTGGAAGAA 
ACTCATAGAT TACTGCAACA GTTCCCCCTG GACTTGGAGA AGTTTCTTGC CTGGCTTACA 
GAAGCTGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GACTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAGG ACCTCCAAGG AGAAATTGAA 
GCTCACACAG ATATCTATCA CAACCTAGAT GAAAATGGCC AAAAAATCCT GAGATCCCTG 
GAAGGTTCTG ATGACGCGGT CCTATTACAA AGACGTTTGG ATAATATGAA TTTCAAGTGG 
AGTGAGCTGC GGAAAAAGTC TCTCAATATC AGGTCCCATT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTGT GGCTACAGCT GAAAGACGAT 
GAGTTGAGCC GTCAGGCACC TATTGGAGGC GATTTCCCAG CAGTTCAGAA GCAGAATGAT 
ATACACAGGG CCTTCAAGAG GGAACTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTGGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCGCC TGAGGAGAAA GCCCAGAATG TCACACGACT CCTACGAAAG 
CAGGCTGAGG AGGTCAACAC AGAGTGGGAA AAATTGAACC TACACTCGGC TGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAAGAACTGC AGGAGGCCAC GGACGAGCTG 
GACCTCAAAC TGCGCCAAGC TGAGGTGATC AAGGGATCAT GGCAGCCCGT GGGCGATCTT 
CTCATTGACT CCCTCCAGGA TCACCTCGAG AAAGTCAAGG CACTTCGAGG AGAAATTGCA 
CCTCTCAAAG AGAATGTAAG CCACGTCAGT GACCTTGCTC GCCAACTCAC CACGTTGGGC 
ATTCAGCTTT CACCATATAA TCTCAGCACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CTGTTGAGGA CCGCATCAGG CAGCTGCATG AAGCCCACAG GGACTTTGGC 
CCAGCGTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCT 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCGGCTTAT 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT TTTGAGCCTG 
TCAGCCGCAT GTGATGCCTT GGACCAGCAC AACCTCAAGC AAAACGACCA GCCCATGGAC 
ATCCTGCAGA TCATTAACTG TTTGACCACT ATTTATGACC GCCTGGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GATACGGGAC GAACAGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCGC ATTTGGAAGA CAAGTACAGA TACCTTTTCA AGCAAGTGGC CAGTTCAACA 
GGATTTTGTG ACCAGCGCAG ACTGGGCCTC CTTCTACATG ACTCTATCCA AATCCCAAGA 
CAGTTGGGTG AAGTTGCATC CTTTGGGGGC AGTAACATTG AGCCAAGTGT CAGGAGCTGC 
TTCCAATTCG CTAATAATAA GCCTGAGATC GAAGCAGCTC TCTTCCTCGA TTGGATGAGA 
CTAGAACCCC AGTCCATGGT GTGGCTGCCC GTCCTGCACA GGGTGGCTGC CGCGGAGACC 
GCCAAGCATC AGGCCAAGTG TAACATCTGC AAGGAGTGTC CAATCATCGG CTTCAGGTAC 
AGAAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGCT GCTTTTTCTC TGGCCGCGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATATT GTACTCCGAC TACATCGGGA 
GAAGATGTTC GAGACTTTGC CAAGGTACTA AAAAACAAAT TTCGCACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACGG TCTTAGAGGG GGACAACATG 
GAAACGCTAG CAGAAATGGA AAACAGCAGT GGATCTTATC TAAATGATAG CATCTCTCCT 
AATGAGAGCA TAGATGATGA ACATTTGTTA ATCCAGCATT ACTGCCAAAG TTTGAACCAG 
GACTCCCCCC TGAGCCAGCC TCGTAGTCCT GCCCAGATCT TGATTTCCTT AGAGAGTGAG 
GAAAGAGGGG AGCTAGAGAG AATCCTAGCA GATCTTGAGG AAGAAAACAG GAATCTGCAG 
GCTGAATATG ATCGTCTCAA GGAGCAGCAT GAACATAAAG GCCTGTCCCC ACTGCCGTCC 
CCTCCTGAGA TGATGCCCAC CTCGCCACAG AGTCCCCGGG ATGCTGAGCT CATTGCTGAG 
GCCAAGCTAC TGCGTCAACA CAAAGGCCGC CTGGAAGCCA GGATGCAAAT CCTGGAAGAC 
CACAATAAAC AGCTGGAGTC CCAGTTACAC AGGCTCAGGC AGCTGCTGGA GCAACCCCAG 
GCTGAGGCCA AGGTGAATGG CACAACGGTG TCCTCTCCTT CTACCTCTCT TCAGAGGTCA 
GACAGCAGCC AGCCTATGCT GCTCCGGGTG GTTGGCAGTC AAACTTCAGA ATCCATGGGC 
GAGGAAGACC TTCTCAGTCC TCCCCAGGAC TCAAGCACAG GGTTAGAGGA AGTGATGGAG 
CAACTCAACA ACTCCTTCCC TAGTTCCAGA GGACACAATG TAGGAAGCCT TTTCCACTTG 
GCAGATGATT TGGGCAGAGC GATGGAGTCC TTAGTTTCAG TTATGACAGA TGAAGAAGGG 
GCAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_024988363.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844131.1 Bos taurus dystrophin (DMD), transcript variant X6, mRNA.

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CloneID	OBa238542
Clone ID Related Accession (Same CDS sequence)	XM_024988363.1
Accession Version	XM_024988363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAATTTTCT AAGTTCGGGA AGCAGCACAT AGAAAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTC TTGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCAGGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAATA TCGGAAGCTC TGACATAGTA 
GATGGAAATC ATAAACTGAC TCTTGGCTTG ATTTGGAATA TAATCCTCCA TTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA GAAGATACTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT CAACTTCACC 
ACCAGCTGGT CTGACGGTTT GGCTTTGAAT GCGCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TCGCCAGCAG TCAGCTACAC AACGACTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTACTGG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TACGTCACAT CTCTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCCTTGAAGC CATCCAGGAG GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACAAGAGAAG AACATTTTCA ACTACATCAT CAAATGCACT ACTCTCAACA GCGTTTGGAA 
GCTCCTGAAG ACAAGTCATT TGGCAGTCCA TTGATGGAGA CTGAAGTAAA CCTGGACAGT 
TACCAGACAG CTTTAGAAGA AGTACTCTCA TGGCTCCTTT CAGCCGAAGA CACATTGCAA 
GCACAAGGAG AGATTTCTAA TGATGTAGAA GAAGTGAAAG AACAGTTTCA TACCCACGAG 
GGATACATGA TGGATTTGAC ATCCCATCAG GGACGGGTTG GCAATGTTCT CCAATTGGGG 
AGTCAGCTGA TTGGGTCAGG GAAATTATCA GAAGATGAAG AAACTGAAGT GCAAGAACAA 
ATGAATCTTC TAAATTCAAG ATGGGAATGC CTCAGGGTAG CTAGCATGGA AAAACAAAGC 
AATTTACATA AAGTTCTCAT GGATCTCCAG AATCAGCAGC TGAAAGAGTT AAATGCCTGG 
TTAACAAAAA CAGAAGAGAG AACAAGGAAA ATGGAAAAAG AGCCCCTTGG ACCTGATCTT 
GAGGACCTCA AACGCCAAAT ACAGCAACAT AAGGTGCTTC AAGAAGACCT AGAACAGGAA 
CAAGTCAGAG TCAATTCTCT CACGCACATG GTGGTGGTTG TAGATGAGTC TAGTGGTGAT 
CATGCAACTG CTGCTTTGGA AGAACAACTT AAGGTACTGG GAGATCGGTG GGCAAACATC 
TGTAGGTGGA CTGAAGATCG CTGGGTTCTT TTACAGGATG TTCTTCTAAA ATGGCAACGT 
TTTACTGAAG AGCAGTGCCT TTTTAGTACA TGGCTTTCAG ATAAAGAAGA TGCATTGAAC 
AAGATTCCCA CAAGTGGCTT TAAGGATCAA AGTGAAATGT TATCAAGTCT TCAAAAACTG 
GCCGTTTTAA AAACTGATCT AGAAAAGAAA AAGCAAACCA TGGACAAACT CTGCTCACTC 
AACCATGATC TCCTTTCAAC ACTGAAAAAT ACATTGGTTG CCCAAAAGAT GGAAGCATGG 
TTGGACAACT TTGCCCAGCG CTGGGATAAT TTAGTCCAAA AACTTGAAAA GAGTTCAACA 
CAGATTTCAC AGGCTGTCAC CACCACTCAG CCATCACTAA CACAGACAAC TGTAATGGAA 
ACGGTAACTA TGGTGACCAC GAGGGAGCAA ATCCTGGTAA AGCATGCCCA AGAGGAACTC 
CCTCCACCAC CTCCACAAAA GAAGAGGCAG ATTATTGTGG ATTCTGAAAT TAAGAAAAGG 
TTGGATGTCG ATATAACTGA ACTTCACAGT TGGATTACTC GTTCAGAAGC CGTGTTACAG 
AGTCCTGAAT TTGCAGTCTA TCGGAAGGAA GGCAACTTCT CAGACCTTAA AGAAAAAGTC 
AATGCCATAG AGCGAGAAAA AGCCGAGAAG TTCAGAAAAC TGCAAGATGC CAGCAGATCA 
GCTCAGGCCC TGGTGGAACA GATGGTGAAT GAGGGTGTTA ATGCTGACAG TATCAAACAA 
GCCTCAGAAC AACTGAACAG CCGGTGGATC GAGTTCTGCC AATTGCTAAG TGAGAGACTT 
AACTGGCTGG AGTATCAGAA CAACATCATC ACTTTCTATA ATCAGCTACA ACAATTGGAG 
CAGATGACAA CTACTGCAGA AAACTGGTTG AAAACTCAAC CCACTACCCC ATCAGAGCCA 
ACAGCAGTTA AAAGCCAGTT AAAAAATTGT AAGGATGAAG TCAACCGGCT GTCAGCTCTT 
CAGCCTCAAA TCGAGAGATT AAAAATTCAA AGCATAGCCC TGAAAGAGAA AGGACAAGGG 
CCAATGTTCC TGGATGCCGA CTTTGTGGCC TTTACAAATC ATTTTAACCA AGTCTTTGCT 
GATATGCAGG CCAGAGAGAA AGAGTTACAG ACAATTTTGG ACACTTTACC CACCGTGCGG 
TATCAGGAGA CCATGAGTAC CATCCTCACG TGGATCCAAC AGTCAGAAAC CAAACTCTCG 
ATACCTCAGG TTACTGTCAC TGAATACGAG ATCATGGAGC AGAGACTTGA GGAGTTACAG 
GCTTTACAAA GCTCTCTGCA AGAGCACCAA AATGACCTAA ACTATCTCAG CACCACTGTG 
AAAGAGATGT CAAGGAAAGC ACCCTCTCAT ATAAGCCAGA GATATCAGTC AGAATTTGAA 
AACATTGAGG GTCGGTGGAA GAAACTCTCT GCCCAGCTGA ATGAACGTTG CCAAAAGCTA 
GAGGAGCAAA TGGCCAAACT CCGAAAACTT CAGAATCACA TAAAAACACT GAAGAAGTGG 
ATGGCTGAAG TTGATGTTTT CTTGAAAGAT GAATGGCCTG CCCTTGGAGA TTCAGAAATT 
CTTAAAAAAC AGCTGAAGCA GTGCAGGCTT TTAGTCAGTG ATATTCAGAC AATCCAGCCC 
AGTCTAAACA GTGTCAATGA AGGTGGACAG AAAATAAAAA CTGAAGCGGA GCCAGAGTTT 
GCTTCTAGAC TGGAGACAGA GCTCAGAGAA CTTAACACTC AATGGGATTA CATATGTCGA 
CAGGTCTATG CCAGAAAGGA GGCCTTAAAG GGAGGCTTGG ATAAAACTGT AAGCCTTCAG 
AAAGATTTGT CAGAGATGCA TGAATGGATG ACACAAGCCG AAGAAGAATA CCTAGAGAGA 
GATTTTGAAT ATAAAACCCC AGATGAATTA CAGACAGCAG TTGAAGAGAT GAAGAGAGCT 
AAAGAAGAGG CCCAGCAAAA AGAAGCAAAA GTGCAGCTCC TCACTGAGTC CGTGAACAGT 
GTCATAGCTC AAGCTCCACC TGCAGCACAA GAGGCCTTAA GAAAGGAACT GGACACTCTG 
ACCACCAACT ACCAGTGGCT CTGCACCAGG CTGAATGGAA AATGCAAGAC GTTAGAAGAG 
GTTTGGGCAT GTTGGCATGA GTTATTGTCA TACTTGGAAA AGGCAAATAA GTGGCTAAAT 
GAAGTAGAAT TGAAACTTAA GACTACTGAA AATATTCCTG GTGGAGCTGA GGAAATCTCT 
GAGGTGCTCA GTTCACTTGA AAATTTGATG CAACATTCAG AAGATAACCC GAATCAGATT 
CGCATATTGG CACAGACCCT AACAGATGGT GGAGTCATGG ATGAACTGAT CAATGAAGAA 
CTCGAGACAT TTAATTCTCG TTGGAGAGAA CTACATGAAG AGGCTGTGAG GAGGCAAAAG 
TTGCTTGAAC AGAGTATCCA GTCTGCCCAG GAGATTGAAA AATCCTTACA GTTAATTCAG 
GAATCCCTCT CATCAATTGA CAAGCAGTTG GCAGCTTATA TCGCTGACAA AGTGGACGCC 
GCTCAGATGC CTCAGGAAGC CCAGAAAATC CAATCTGATT TGACAAGTCA TGAGATCAGT 
TTAGAAGAAA TGAAGAAACA TTACCAAGGA AAGGAAACTG CTCAACGGGT ACTATCCCAA 
ATTGAAGTGG CACAGAAAAA AATGCAAGAT GTTTCCATGA AATTTCGATT GTTCCAGAAG 
CCAGCCAATT TTGAGCAGCG TCTACAGGAA AGTAAGATGA TTTTAGATGA AGTGAAGATG 
CACTTGCCCG CACTGGAAAC AAAGAGTGTT GAACAGGAAG TAGTACAGTC ACAGTTAAAT 
CATTGTGTGA ACTTGTATAA AAGTCTGAGT GAAGTGAAGT CTGAAGTGGA AATGGTGATT 
AAAACTGGAC GTCAAATTGT ACAGAAGAAG CAGACGGAAA ACCCTAAAGA GCTTGATGAA 
AGAGTAACGG CTTTGAAATT GCATTATAAT GAGCTGGGAG CAAAGGTAAC AGAAAGAAAG 
CAACAGTTGG AGAAATGCTT GAAATTGTCC CGTAAGATGC GAAAGGAAAT GAATGCCTTG 
ACAGAATGGC TGGCAGCTAC AGATTTGGAA CTGACAAAGA GATCAGCAGT TGAAGGAATG 
CCTAGTAATT TGGATTCTGA AGTTGCCTGG GGAAAGGCTA CTCAGAAAGA GACTGAGAAA 
CAGAAGGTTC ACCTAAAGAG CATTACAGAG CTAGGAGAGG CCTTGAAAAC GGTTTTGGGC 
AAGAAGGAGA CCTTGGTCGA AGATAAACTG AGTCTTTTGA ATAGTAACTG GATAGCCGTC 
ACTTCTCGAG CAGAAGAGTG GCTAAACCTT TTGTTGGAAT ACCAGAAACA CATGGAAACC 
TTTGACCAGA ATGTGGATCA TATCACAAAG TGGATCATTC AAGCTGATGC ACTTCTGGAT 
GAATCTGAGA AAAAGAAACC ACAGCAAAAA GAAGACATGC TAAAGCGCTT AAAGGCTGAA 
CTGAATGACA TACGCCCAAA GGTGGACTCC ACGCGTGACC AAGCAGCAAA CTTGATGGCA 
AACCGCGGCG ACCACTGCAG GAAAGTAATA GAGCCCAAAA TCTCAGAGCT CAACCATCGA 
TTTGCAGCCA TTTCTCACAG AATTAAGACT GGAAAGGCCT CCATTCCTTT GAAGGAATTG 
GAGCAGTTTA ACTCAGATAT ACAAAAATTG CTTGAACCAC TGGAGGCTGA AATTCAGCAG 
GGGGTGAATC TGAAAGAGGA AGACTTCAAT AAAGATATGA GTGAAGACAA TGAGGGTACT 
GTAAAAGAAT TGTTGCAAAG AGGAGACAAC TTACAACAAA GAATCACAGA TGAGAGAAAG 
CGAGAGGAAA TAAAGATAAA ACAGCAGCTG TTACAGACAA AACATAATGC TCTCAAGGAT 
TTGAGGTCTC AAAGAAGAAA AAAGGCTCTA GAAATTTCTC ACCAGTGGTA TCAGTACAAG 
AGGCAGGCTG ATGATCTCCT GAAATGCTTG GATGACATTG AAAAAAAATT AGCCAGCCTA 
CCTGAACCCA GAGATGAAAG GAAAATAAAG GAAATTGATC GTGAGTTGCA GAAGAAGAAA 
GAGGAGCTGA ATGCAGTGCA TAGGCAAGCT GAGGGCTTGT CTGAGGATGG GGCAGCAATG 
GCAGTGGAGT CAACTCAGAT CCAGCTCAGC AAGCGCTGGC GGGAAATTGA GAGCAAATTT 
GCTCAGTTTC GAAGACTCAA CTTTGCACAA ATTCACACTG TCCATGAAGA ATCAGTGATG 
GTGATGACCG AAGACATGCC TTTGGAAATT TCTTATGTGC CTTCTACTTA CCTGACTGAG 
ATCACTCACG TCTTACAAGC CCTTTCAGAA GTGGAACAAC TTCTCAATGC TCCCGACCTC 
TGTGCTAAAG ATTTTCAAGA TCTCTTCAAA CAAGAGGAAT CTCTGAAGAA TATAAAAGAC 
AACCTGCAAC AAATCTCAGG TCGGATTGAT GTTATTCACA ATAAGAAGGC GGCAGCATTG 
CAAAGCACCA CACCCCCAGA AAAGGCAAGG TTACAGGAAG CTCTCTCCAG GCTCGACTTC 
CAATGGGAAA GAGTGAACAA AATGTACAAG GACCGACAAG GGCAATTTGA CAGATCTGTT 
GAAAAATGGC GACGGTTTCA TTATGATATG AAGATATTTA ATCAATGGCT AACAGAAGCT 
GAACACTTTC TCAAAAAGAC ACAAATTCCT GAGAACTGGG AACATGCCAA ATATAAATGG 
TATCTTAAGG AACTCCAGGA TGGCATCGGG CAGCGGCAAA CTGTTGTCAG AGTATTGAAT 
GCAACTGGGG AAGAAATAAT TCAGCAGTCC TCAAAAATAG ATGCCAGTAT TCTACAAGAA 
AAATTGGGAA GCCTGAATCT GCGGTGGCAG GAGGTCTGCA AACAGCTGGC CGAGAGAAAA 
AAGAGGTTAG AAGAACAAAA GAATATCCTG TCAGAATTTC AAAGAGATTT GAATGAATTT 
GTTTTATGGT TGGAAGAAGC AGGTAACATT TCTAGTATTC CTCTTGAACC TGGAAATGAG 
CAGCAACTAA AAGAAAAGCT TGAGGAAGTC AAGTTACTGG CCGAAGAGTT GCCCCTGCGT 
CAGGGAATTC TAAAACAATT AAATGAAACA GGAGGAACAG TGCTTGTAAG TGCTCCCATA 
AGCCCAGAAG AGCAAGATAA ACTTGAAAAT AAGCTCAAGC AGACAAATCT TCAGTGGATA 
AAGGTTTCTA GGAGTTTACC TGAGAAACAG GGAGAAATTG AGGCTCACAT AAAAGACCTT 
GGACAGCTTG AAGAGCAATT AAATCATCTG CTTCTGTGGC TGTCTCCTAT TAGGAGTCAG 
TTGGAAATTT ACAACCAACC AAATCAGACA GGACCATTTG ACATTAAGGA AACTGAAGTA 
GCAGTTCAAG CTAAACAGCC GGATGTGGAA GGGATTTTGT CTAAAGGGCA GAATTTGTAC 
AAGGAAAAAC CAGCCACTGA GCCAGTGAAG AGGAAGTTAG AAGATCTGAG CTCGGAGTGG 
AAGGCGGTAA CCCACTTACT TCAAGAGCTG AGGGCAAAGT GGCCTGGCCC AGTCCCTGGA 
CTGACAGCAA CTGGAGCACC TCCTAGTCAG ACGGTTGCTC TGGTGACACA ACCTGTGGTT 
GCCAAGGAAA CCGCTGTCTC CAAACTAGAA ATGCCATCTT CCTTGCTGTT GGAGGTCCCT 
GCACTGGCAG ATTTCAACCG GGCTTGGACA GAACTTACCG ACTGGCTGTC TCTGCTTGAT 
CGAGTTCTGA AAGCACAGAG GGTGATGGTG GGTGATCTTG AAGACATCAA CGAGATGATC 
ATCAAGCAGA AGGCAACACT GCAGGACTTG GAGCAGAGGC GCCCCCAGTT GGAAGAACTC 
ATTACTGCTG CCCAGAATTT GAAAAACAAG ACCAGCAATC AAGAGGCCAG GACCATCATT 
ACTGATCGAA TTGAAAGAAT TCAGAGTCAG TGGGATGAAG TACAGGAACA CCTTCAGAAC 
CGGAGGCAGC AGTTAAATGA AATGTTAAAG GATTCAACAC AATGGCTGGA AGCTAAGGAA 
GAAGCTGAGC AGGTGGTGGG GCAGGCCAGA GCCAAGCTTG AGACATGGAA GGAGGGTCCC 
TACACAATGG ATGCAATCCA AAGGAAGATC ACAGAAACCA AGCAGTTGGC CAAAGACCTC 
CGCCAGTGGC AGATAAATGT AGATGTGGCA AATGACTTGG CACTGAAACT TCTCCGGGAT 
TATTCTGCAG ATGATACCCG AAAGGTACAC ATGATAACAG AGAACATCAA TGCCTCTTGG 
GCAAGCATTC ATAAAAGAGT GAGTGAGCGA GAGACTGCTC TGGAAGAAAC TCATAGATTA 
CTGCAACAGT TCCCCCTGGA CTTGGAGAAG TTTCTTGCCT GGCTTACAGA AGCTGAAACA 
ACTGCCAACG TCCTGCAGGA TGCCACCCAT AAGGAAAGGC TTCTAGAAGA CTCCAAGGGA 
GTAAGAGAGC TGATGAAACA ATGGCAGGAC CTCCAAGGAG AAATTGAAGC TCACACAGAT 
ATCTATCACA ACCTAGATGA AAATGGCCAA AAAATCCTGA GATCCCTGGA AGGTTCTGAT 
GACGCGGTCC TATTACAAAG ACGTTTGGAT AATATGAATT TCAAGTGGAG TGAGCTGCGG 
AAAAAGTCTC TCAATATCAG GTCCCATTTG GAAGCCAGTT CTGACCAGTG GAAGCGTCTG 
CACCTTTCTC TTCAGGAACT TCTGGTGTGG CTACAGCTGA AAGACGATGA GTTGAGCCGT 
CAGGCACCTA TTGGAGGCGA TTTCCCAGCA GTTCAGAAGC AGAATGATAT ACACAGGGCC 
TTCAAGAGGG AACTGAAAAC GAAAGAACCT GTAATCATGA GTACTCTTGA GACTGTACGA 
ATATTTCTGA CAGAGCAGCC TTTGGAAGGA CTAGAGAAAC TCTACCAGGA GCCCAGAGAG 
CTGCCGCCTG AGGAGAAAGC CCAGAATGTC ACACGACTCC TACGAAAGCA GGCTGAGGAG 
GTCAACACAG AGTGGGAAAA ATTGAACCTA CACTCGGCTG ACTGGCAGAG AAAAATAGAC 
GAGGCCCTCG AAAGACTCCA AGAACTGCAG GAGGCCACGG ACGAGCTGGA CCTCAAACTG 
CGCCAAGCTG AGGTGATCAA GGGATCATGG CAGCCCGTGG GCGATCTTCT CATTGACTCC 
CTCCAGGATC ACCTCGAGAA AGTCAAGGCA CTTCGAGGAG AAATTGCACC TCTCAAAGAG 
AATGTAAGCC ACGTCAGTGA CCTTGCTCGC CAACTCACCA CGTTGGGCAT TCAGCTTTCA 
CCATATAATC TCAGCACTCT GGAAGACCTG AACACCAGAT GGAAGCTTCT GCAGGTGGCT 
GTTGAGGACC GCATCAGGCA GCTGCATGAA GCCCACAGGG ACTTTGGCCC AGCGTCCCAG 
CACTTCCTTT CCACTTCTGT CCAGGGTCCC TGGGAGAGAG CCATCTCACC AAACAAAGTG 
CCCTACTATA TCAACCACGA GACCCAAACA ACTTGCTGGG ACCATCCTAA AATGACAGAG 
CTCTACCAGT CTTTAGCTGA CCTGAATAAT GTCAGGTTCT CGGCTTATAG GACTGCCATG 
AAACTCCGAA GACTGCAGAA GGCCCTTTGC TTGGATCTTT TGAGCCTGTC AGCCGCATGT 
GATGCCTTGG ACCAGCACAA CCTCAAGCAA AACGACCAGC CCATGGACAT CCTGCAGATC 
ATTAACTGTT TGACCACTAT TTATGACCGC CTGGAGCAAG AGCACAACAA TCTGGTCAAC 
GTCCCTCTCT GCGTGGATAT GTGTCTCAAT TGGCTGCTGA ATGTTTATGA TACGGGACGA 
ACAGGGAGGA TCCGGGTCCT GTCTTTTAAA ACTGGCATCA TTTCTCTGTG TAAAGCGCAT 
TTGGAAGACA AGTACAGATA CCTTTTCAAG CAAGTGGCCA GTTCAACAGG ATTTTGTGAC 
CAGCGCAGAC TGGGCCTCCT TCTACATGAC TCTATCCAAA TCCCAAGACA GTTGGGTGAA 
GTTGCATCCT TTGGGGGCAG TAACATTGAG CCAAGTGTCA GGAGCTGCTT CCAATTCGCT 
AATAATAAGC CTGAGATCGA AGCAGCTCTC TTCCTCGATT GGATGAGACT AGAACCCCAG 
TCCATGGTGT GGCTGCCCGT CCTGCACAGG GTGGCTGCCG CGGAGACCGC CAAGCATCAG 
GCCAAGTGTA ACATCTGCAA GGAGTGTCCA ATCATCGGCT TCAGGTACAG AAGTCTAAAG 
CACTTTAATT ATGACATCTG CCAAAGCTGC TTTTTCTCTG GCCGCGTTGC AAAAGGCCAT 
AAAATGCACT ATCCCATGGT GGAATATTGT ACTCCGACTA CATCGGGAGA AGATGTTCGA 
GACTTTGCCA AGGTACTAAA AAACAAATTT CGCACCAAAA GGTATTTTGC GAAGCATCCC 
CGAATGGGCT ACCTGCCAGT GCAGACGGTC TTAGAGGGGG ACAACATGGA AACTCCTGTT 
ACTCTGATCA ACTTCTGGCC GGTAGACTCT GCGCCTGCCT CGTCCCCTCA GCTTTCACAC 
GATGATACTC ATTCACGCAT TGAACATTAT GCTAGCAGGC TAGCAGAAAT GGAAAACAGC 
AGTGGATCTT ATCTAAATGA TAGCATCTCT CCTAATGAGA GCATAGATGA TGAACATTTG 
TTAATCCAGC ATTACTGCCA AAGTTTGAAC CAGGACTCCC CCCTGAGCCA GCCTCGTAGT 
CCTGCCCAGA TCTTGATTTC CTTAGAGAGT GAGGAAAGAG GGGAGCTAGA GAGAATCCTA 
GCAGATCTTG AGGAAGAAAA CAGGAATCTG CAGGCTGAAT ATGATCGTCT CAAGGAGCAG 
CATGAACATA AAGGCCTGTC CCCACTGCCG TCCCCTCCTG AGATGATGCC CACCTCGCCA 
CAGAGTCCCC GGGATGCTGA GCTCATTGCT GAGGCCAAGC TACTGCGTCA ACACAAAGGC 
CGCCTGGAAG CCAGGATGCA AATCCTGGAA GACCACAATA AACAGCTGGA GTCCCAGTTA 
CACAGGCTCA GGCAGCTGCT GGAGCAACCC CAGGCTGAGG CCAAGGTGAA TGGCACAACG 
GTGTCCTCTC CTTCTACCTC TCTTCAGAGG TCAGACAGCA GCCAGCCTAT GCTGCTCCGG 
GTGGTTGGCA GTCAAACTTC AGAATCCATG GGCGAGGAAG ACCTTCTCAG TCCTCCCCAG 
GACTCAAGCA CAGGGTTAGA GGAAGTGATG GAGCAACTCA ACAACTCCTT CCCTAGTTCC 
AGAGGACACA ATGTAGGAAG CCTTTTCCAC TTGGCAGATG ATTTGGGCAG AGCGATGGAG 
TCCTTAGTTT CAGTTATGAC AGATGAAGAA GGGGCAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844131.1
CDS	251..11212
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALQVLQKNNVDLVNIGSSDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVRQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYVTSLFQVLPQQVSLEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQRLEAPEDKSFGSPLMETEVNLDSYQTALEEVLSWLLSAEDTLQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGSGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNAWLTKTEERTRKMEKEPLGPDLEDLKRQIQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDVLLKWQRFTEEQCLFSTWLSDKEDALNKIPTSGFKDQSEMLSSLQKLAVLKTDLEKKKQTMDKLCSLNHDLLSTLKNTLVAQKMEAWLDNFAQRWDNLVQKLEKSSTQISQAVTTTQPSLTQTTVMETVTMVTTREQILVKHAQEELPPPPPQKKRQIIVDSEIKKRLDVDITELHSWITRSEAVLQSPEFAVYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTPSEPTAVKSQLKNCKDEVNRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADMQAREKELQTILDTLPTVRYQETMSTILTWIQQSETKLSIPQVTVTEYEIMEQRLEELQALQSSLQEHQNDLNYLSTTVKEMSRKAPSHISQRYQSEFENIEGRWKKLSAQLNERCQKLEEQMAKLRKLQNHIKTLKKWMAEVDVFLKDEWPALGDSEILKKQLKQCRLLVSDIQTIQPSLNSVNEGGQKIKTEAEPEFASRLETELRELNTQWDYICRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVQLLTESVNSVIAQAPPAAQEALRKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLNEVELKLKTTENIPGGAEEISEVLSSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLQLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHYQGKETAQRVLSQIEVAQKKMQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDLELTKRSAVEGMPSNLDSEVAWGKATQKETEKQKVHLKSITELGEALKTVLGKKETLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMETFDQNVDHITKWIIQADALLDESEKKKPQQKEDMLKRLKAELNDIRPKVDSTRDQAANLMANRGDHCRKVIEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVHRQAEGLSEDGAAMAVESTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVMVMTEDMPLEISYVPSTYLTEITHVLQALSEVEQLLNAPDLCAKDFQDLFKQEESLKNIKDNLQQISGRIDVIHNKKAAALQSTTPPEKARLQEALSRLDFQWERVNKMYKDRQGQFDRSVEKWRRFHYDMKIFNQWLTEAEHFLKKTQIPENWEHAKYKWYLKELQDGIGQRQTVVRVLNATGEEIIQQSSKIDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDLNEFVLWLEEAGNISSIPLEPGNEQQLKEKLEEVKLLAEELPLRQGILKQLNETGGTVLVSAPISPEEQDKLENKLKQTNLQWIKVSRSLPEKQGEIEAHIKDLGQLEEQLNHLLLWLSPIRSQLEIYNQPNQTGPFDIKETEVAVQAKQPDVEGILSKGQNLYKEKPATEPVKRKLEDLSSEWKAVTHLLQELRAKWPGPVPGLTATGAPPSQTVALVTQPVVAKETAVSKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVLKAQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVVGQARAKLETWKEGPYTMDAIQRKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRVSERETALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKILRSLEGSDDAVLLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDIHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEEKAQNVTRLLRKQAEEVNTEWEKLNLHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVSHVSDLARQLTTLGIQLSPYNLSTLEDLNTRWKLLQVAVEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSSGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKEQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDSSTGLEEVMEQLNNSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_024988363.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophin (DMD), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988363.1

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAATTTTCT AAGTTCGGGA AGCAGCACAT AGAAAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTC TTGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCAGGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAATA TCGGAAGCTC TGACATAGTA 
GATGGAAATC ATAAACTGAC TCTTGGCTTG ATTTGGAATA TAATCCTCCA TTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA GAAGATACTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT CAACTTCACC 
ACCAGCTGGT CTGACGGTTT GGCTTTGAAT GCGCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TCGCCAGCAG TCAGCTACAC AACGACTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTACTGG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TACGTCACAT CTCTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCCTTGAAGC CATCCAGGAG GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACAAGAGAAG AACATTTTCA ACTACATCAT CAAATGCACT ACTCTCAACA GCGTTTGGAA 
GCTCCTGAAG ACAAGTCATT TGGCAGTCCA TTGATGGAGA CTGAAGTAAA CCTGGACAGT 
TACCAGACAG CTTTAGAAGA AGTACTCTCA TGGCTCCTTT CAGCCGAAGA CACATTGCAA 
GCACAAGGAG AGATTTCTAA TGATGTAGAA GAAGTGAAAG AACAGTTTCA TACCCACGAG 
GGATACATGA TGGATTTGAC ATCCCATCAG GGACGGGTTG GCAATGTTCT CCAATTGGGG 
AGTCAGCTGA TTGGGTCAGG GAAATTATCA GAAGATGAAG AAACTGAAGT GCAAGAACAA 
ATGAATCTTC TAAATTCAAG ATGGGAATGC CTCAGGGTAG CTAGCATGGA AAAACAAAGC 
AATTTACATA AAGTTCTCAT GGATCTCCAG AATCAGCAGC TGAAAGAGTT AAATGCCTGG 
TTAACAAAAA CAGAAGAGAG AACAAGGAAA ATGGAAAAAG AGCCCCTTGG ACCTGATCTT 
GAGGACCTCA AACGCCAAAT ACAGCAACAT AAGGTGCTTC AAGAAGACCT AGAACAGGAA 
CAAGTCAGAG TCAATTCTCT CACGCACATG GTGGTGGTTG TAGATGAGTC TAGTGGTGAT 
CATGCAACTG CTGCTTTGGA AGAACAACTT AAGGTACTGG GAGATCGGTG GGCAAACATC 
TGTAGGTGGA CTGAAGATCG CTGGGTTCTT TTACAGGATG TTCTTCTAAA ATGGCAACGT 
TTTACTGAAG AGCAGTGCCT TTTTAGTACA TGGCTTTCAG ATAAAGAAGA TGCATTGAAC 
AAGATTCCCA CAAGTGGCTT TAAGGATCAA AGTGAAATGT TATCAAGTCT TCAAAAACTG 
GCCGTTTTAA AAACTGATCT AGAAAAGAAA AAGCAAACCA TGGACAAACT CTGCTCACTC 
AACCATGATC TCCTTTCAAC ACTGAAAAAT ACATTGGTTG CCCAAAAGAT GGAAGCATGG 
TTGGACAACT TTGCCCAGCG CTGGGATAAT TTAGTCCAAA AACTTGAAAA GAGTTCAACA 
CAGATTTCAC AGGCTGTCAC CACCACTCAG CCATCACTAA CACAGACAAC TGTAATGGAA 
ACGGTAACTA TGGTGACCAC GAGGGAGCAA ATCCTGGTAA AGCATGCCCA AGAGGAACTC 
CCTCCACCAC CTCCACAAAA GAAGAGGCAG ATTATTGTGG ATTCTGAAAT TAAGAAAAGG 
TTGGATGTCG ATATAACTGA ACTTCACAGT TGGATTACTC GTTCAGAAGC CGTGTTACAG 
AGTCCTGAAT TTGCAGTCTA TCGGAAGGAA GGCAACTTCT CAGACCTTAA AGAAAAAGTC 
AATGCCATAG AGCGAGAAAA AGCCGAGAAG TTCAGAAAAC TGCAAGATGC CAGCAGATCA 
GCTCAGGCCC TGGTGGAACA GATGGTGAAT GAGGGTGTTA ATGCTGACAG TATCAAACAA 
GCCTCAGAAC AACTGAACAG CCGGTGGATC GAGTTCTGCC AATTGCTAAG TGAGAGACTT 
AACTGGCTGG AGTATCAGAA CAACATCATC ACTTTCTATA ATCAGCTACA ACAATTGGAG 
CAGATGACAA CTACTGCAGA AAACTGGTTG AAAACTCAAC CCACTACCCC ATCAGAGCCA 
ACAGCAGTTA AAAGCCAGTT AAAAAATTGT AAGGATGAAG TCAACCGGCT GTCAGCTCTT 
CAGCCTCAAA TCGAGAGATT AAAAATTCAA AGCATAGCCC TGAAAGAGAA AGGACAAGGG 
CCAATGTTCC TGGATGCCGA CTTTGTGGCC TTTACAAATC ATTTTAACCA AGTCTTTGCT 
GATATGCAGG CCAGAGAGAA AGAGTTACAG ACAATTTTGG ACACTTTACC CACCGTGCGG 
TATCAGGAGA CCATGAGTAC CATCCTCACG TGGATCCAAC AGTCAGAAAC CAAACTCTCG 
ATACCTCAGG TTACTGTCAC TGAATACGAG ATCATGGAGC AGAGACTTGA GGAGTTACAG 
GCTTTACAAA GCTCTCTGCA AGAGCACCAA AATGACCTAA ACTATCTCAG CACCACTGTG 
AAAGAGATGT CAAGGAAAGC ACCCTCTCAT ATAAGCCAGA GATATCAGTC AGAATTTGAA 
AACATTGAGG GTCGGTGGAA GAAACTCTCT GCCCAGCTGA ATGAACGTTG CCAAAAGCTA 
GAGGAGCAAA TGGCCAAACT CCGAAAACTT CAGAATCACA TAAAAACACT GAAGAAGTGG 
ATGGCTGAAG TTGATGTTTT CTTGAAAGAT GAATGGCCTG CCCTTGGAGA TTCAGAAATT 
CTTAAAAAAC AGCTGAAGCA GTGCAGGCTT TTAGTCAGTG ATATTCAGAC AATCCAGCCC 
AGTCTAAACA GTGTCAATGA AGGTGGACAG AAAATAAAAA CTGAAGCGGA GCCAGAGTTT 
GCTTCTAGAC TGGAGACAGA GCTCAGAGAA CTTAACACTC AATGGGATTA CATATGTCGA 
CAGGTCTATG CCAGAAAGGA GGCCTTAAAG GGAGGCTTGG ATAAAACTGT AAGCCTTCAG 
AAAGATTTGT CAGAGATGCA TGAATGGATG ACACAAGCCG AAGAAGAATA CCTAGAGAGA 
GATTTTGAAT ATAAAACCCC AGATGAATTA CAGACAGCAG TTGAAGAGAT GAAGAGAGCT 
AAAGAAGAGG CCCAGCAAAA AGAAGCAAAA GTGCAGCTCC TCACTGAGTC CGTGAACAGT 
GTCATAGCTC AAGCTCCACC TGCAGCACAA GAGGCCTTAA GAAAGGAACT GGACACTCTG 
ACCACCAACT ACCAGTGGCT CTGCACCAGG CTGAATGGAA AATGCAAGAC GTTAGAAGAG 
GTTTGGGCAT GTTGGCATGA GTTATTGTCA TACTTGGAAA AGGCAAATAA GTGGCTAAAT 
GAAGTAGAAT TGAAACTTAA GACTACTGAA AATATTCCTG GTGGAGCTGA GGAAATCTCT 
GAGGTGCTCA GTTCACTTGA AAATTTGATG CAACATTCAG AAGATAACCC GAATCAGATT 
CGCATATTGG CACAGACCCT AACAGATGGT GGAGTCATGG ATGAACTGAT CAATGAAGAA 
CTCGAGACAT TTAATTCTCG TTGGAGAGAA CTACATGAAG AGGCTGTGAG GAGGCAAAAG 
TTGCTTGAAC AGAGTATCCA GTCTGCCCAG GAGATTGAAA AATCCTTACA GTTAATTCAG 
GAATCCCTCT CATCAATTGA CAAGCAGTTG GCAGCTTATA TCGCTGACAA AGTGGACGCC 
GCTCAGATGC CTCAGGAAGC CCAGAAAATC CAATCTGATT TGACAAGTCA TGAGATCAGT 
TTAGAAGAAA TGAAGAAACA TTACCAAGGA AAGGAAACTG CTCAACGGGT ACTATCCCAA 
ATTGAAGTGG CACAGAAAAA AATGCAAGAT GTTTCCATGA AATTTCGATT GTTCCAGAAG 
CCAGCCAATT TTGAGCAGCG TCTACAGGAA AGTAAGATGA TTTTAGATGA AGTGAAGATG 
CACTTGCCCG CACTGGAAAC AAAGAGTGTT GAACAGGAAG TAGTACAGTC ACAGTTAAAT 
CATTGTGTGA ACTTGTATAA AAGTCTGAGT GAAGTGAAGT CTGAAGTGGA AATGGTGATT 
AAAACTGGAC GTCAAATTGT ACAGAAGAAG CAGACGGAAA ACCCTAAAGA GCTTGATGAA 
AGAGTAACGG CTTTGAAATT GCATTATAAT GAGCTGGGAG CAAAGGTAAC AGAAAGAAAG 
CAACAGTTGG AGAAATGCTT GAAATTGTCC CGTAAGATGC GAAAGGAAAT GAATGCCTTG 
ACAGAATGGC TGGCAGCTAC AGATTTGGAA CTGACAAAGA GATCAGCAGT TGAAGGAATG 
CCTAGTAATT TGGATTCTGA AGTTGCCTGG GGAAAGGCTA CTCAGAAAGA GACTGAGAAA 
CAGAAGGTTC ACCTAAAGAG CATTACAGAG CTAGGAGAGG CCTTGAAAAC GGTTTTGGGC 
AAGAAGGAGA CCTTGGTCGA AGATAAACTG AGTCTTTTGA ATAGTAACTG GATAGCCGTC 
ACTTCTCGAG CAGAAGAGTG GCTAAACCTT TTGTTGGAAT ACCAGAAACA CATGGAAACC 
TTTGACCAGA ATGTGGATCA TATCACAAAG TGGATCATTC AAGCTGATGC ACTTCTGGAT 
GAATCTGAGA AAAAGAAACC ACAGCAAAAA GAAGACATGC TAAAGCGCTT AAAGGCTGAA 
CTGAATGACA TACGCCCAAA GGTGGACTCC ACGCGTGACC AAGCAGCAAA CTTGATGGCA 
AACCGCGGCG ACCACTGCAG GAAAGTAATA GAGCCCAAAA TCTCAGAGCT CAACCATCGA 
TTTGCAGCCA TTTCTCACAG AATTAAGACT GGAAAGGCCT CCATTCCTTT GAAGGAATTG 
GAGCAGTTTA ACTCAGATAT ACAAAAATTG CTTGAACCAC TGGAGGCTGA AATTCAGCAG 
GGGGTGAATC TGAAAGAGGA AGACTTCAAT AAAGATATGA GTGAAGACAA TGAGGGTACT 
GTAAAAGAAT TGTTGCAAAG AGGAGACAAC TTACAACAAA GAATCACAGA TGAGAGAAAG 
CGAGAGGAAA TAAAGATAAA ACAGCAGCTG TTACAGACAA AACATAATGC TCTCAAGGAT 
TTGAGGTCTC AAAGAAGAAA AAAGGCTCTA GAAATTTCTC ACCAGTGGTA TCAGTACAAG 
AGGCAGGCTG ATGATCTCCT GAAATGCTTG GATGACATTG AAAAAAAATT AGCCAGCCTA 
CCTGAACCCA GAGATGAAAG GAAAATAAAG GAAATTGATC GTGAGTTGCA GAAGAAGAAA 
GAGGAGCTGA ATGCAGTGCA TAGGCAAGCT GAGGGCTTGT CTGAGGATGG GGCAGCAATG 
GCAGTGGAGT CAACTCAGAT CCAGCTCAGC AAGCGCTGGC GGGAAATTGA GAGCAAATTT 
GCTCAGTTTC GAAGACTCAA CTTTGCACAA ATTCACACTG TCCATGAAGA ATCAGTGATG 
GTGATGACCG AAGACATGCC TTTGGAAATT TCTTATGTGC CTTCTACTTA CCTGACTGAG 
ATCACTCACG TCTTACAAGC CCTTTCAGAA GTGGAACAAC TTCTCAATGC TCCCGACCTC 
TGTGCTAAAG ATTTTCAAGA TCTCTTCAAA CAAGAGGAAT CTCTGAAGAA TATAAAAGAC 
AACCTGCAAC AAATCTCAGG TCGGATTGAT GTTATTCACA ATAAGAAGGC GGCAGCATTG 
CAAAGCACCA CACCCCCAGA AAAGGCAAGG TTACAGGAAG CTCTCTCCAG GCTCGACTTC 
CAATGGGAAA GAGTGAACAA AATGTACAAG GACCGACAAG GGCAATTTGA CAGATCTGTT 
GAAAAATGGC GACGGTTTCA TTATGATATG AAGATATTTA ATCAATGGCT AACAGAAGCT 
GAACACTTTC TCAAAAAGAC ACAAATTCCT GAGAACTGGG AACATGCCAA ATATAAATGG 
TATCTTAAGG AACTCCAGGA TGGCATCGGG CAGCGGCAAA CTGTTGTCAG AGTATTGAAT 
GCAACTGGGG AAGAAATAAT TCAGCAGTCC TCAAAAATAG ATGCCAGTAT TCTACAAGAA 
AAATTGGGAA GCCTGAATCT GCGGTGGCAG GAGGTCTGCA AACAGCTGGC CGAGAGAAAA 
AAGAGGTTAG AAGAACAAAA GAATATCCTG TCAGAATTTC AAAGAGATTT GAATGAATTT 
GTTTTATGGT TGGAAGAAGC AGGTAACATT TCTAGTATTC CTCTTGAACC TGGAAATGAG 
CAGCAACTAA AAGAAAAGCT TGAGGAAGTC AAGTTACTGG CCGAAGAGTT GCCCCTGCGT 
CAGGGAATTC TAAAACAATT AAATGAAACA GGAGGAACAG TGCTTGTAAG TGCTCCCATA 
AGCCCAGAAG AGCAAGATAA ACTTGAAAAT AAGCTCAAGC AGACAAATCT TCAGTGGATA 
AAGGTTTCTA GGAGTTTACC TGAGAAACAG GGAGAAATTG AGGCTCACAT AAAAGACCTT 
GGACAGCTTG AAGAGCAATT AAATCATCTG CTTCTGTGGC TGTCTCCTAT TAGGAGTCAG 
TTGGAAATTT ACAACCAACC AAATCAGACA GGACCATTTG ACATTAAGGA AACTGAAGTA 
GCAGTTCAAG CTAAACAGCC GGATGTGGAA GGGATTTTGT CTAAAGGGCA GAATTTGTAC 
AAGGAAAAAC CAGCCACTGA GCCAGTGAAG AGGAAGTTAG AAGATCTGAG CTCGGAGTGG 
AAGGCGGTAA CCCACTTACT TCAAGAGCTG AGGGCAAAGT GGCCTGGCCC AGTCCCTGGA 
CTGACAGCAA CTGGAGCACC TCCTAGTCAG ACGGTTGCTC TGGTGACACA ACCTGTGGTT 
GCCAAGGAAA CCGCTGTCTC CAAACTAGAA ATGCCATCTT CCTTGCTGTT GGAGGTCCCT 
GCACTGGCAG ATTTCAACCG GGCTTGGACA GAACTTACCG ACTGGCTGTC TCTGCTTGAT 
CGAGTTCTGA AAGCACAGAG GGTGATGGTG GGTGATCTTG AAGACATCAA CGAGATGATC 
ATCAAGCAGA AGGCAACACT GCAGGACTTG GAGCAGAGGC GCCCCCAGTT GGAAGAACTC 
ATTACTGCTG CCCAGAATTT GAAAAACAAG ACCAGCAATC AAGAGGCCAG GACCATCATT 
ACTGATCGAA TTGAAAGAAT TCAGAGTCAG TGGGATGAAG TACAGGAACA CCTTCAGAAC 
CGGAGGCAGC AGTTAAATGA AATGTTAAAG GATTCAACAC AATGGCTGGA AGCTAAGGAA 
GAAGCTGAGC AGGTGGTGGG GCAGGCCAGA GCCAAGCTTG AGACATGGAA GGAGGGTCCC 
TACACAATGG ATGCAATCCA AAGGAAGATC ACAGAAACCA AGCAGTTGGC CAAAGACCTC 
CGCCAGTGGC AGATAAATGT AGATGTGGCA AATGACTTGG CACTGAAACT TCTCCGGGAT 
TATTCTGCAG ATGATACCCG AAAGGTACAC ATGATAACAG AGAACATCAA TGCCTCTTGG 
GCAAGCATTC ATAAAAGAGT GAGTGAGCGA GAGACTGCTC TGGAAGAAAC TCATAGATTA 
CTGCAACAGT TCCCCCTGGA CTTGGAGAAG TTTCTTGCCT GGCTTACAGA AGCTGAAACA 
ACTGCCAACG TCCTGCAGGA TGCCACCCAT AAGGAAAGGC TTCTAGAAGA CTCCAAGGGA 
GTAAGAGAGC TGATGAAACA ATGGCAGGAC CTCCAAGGAG AAATTGAAGC TCACACAGAT 
ATCTATCACA ACCTAGATGA AAATGGCCAA AAAATCCTGA GATCCCTGGA AGGTTCTGAT 
GACGCGGTCC TATTACAAAG ACGTTTGGAT AATATGAATT TCAAGTGGAG TGAGCTGCGG 
AAAAAGTCTC TCAATATCAG GTCCCATTTG GAAGCCAGTT CTGACCAGTG GAAGCGTCTG 
CACCTTTCTC TTCAGGAACT TCTGGTGTGG CTACAGCTGA AAGACGATGA GTTGAGCCGT 
CAGGCACCTA TTGGAGGCGA TTTCCCAGCA GTTCAGAAGC AGAATGATAT ACACAGGGCC 
TTCAAGAGGG AACTGAAAAC GAAAGAACCT GTAATCATGA GTACTCTTGA GACTGTACGA 
ATATTTCTGA CAGAGCAGCC TTTGGAAGGA CTAGAGAAAC TCTACCAGGA GCCCAGAGAG 
CTGCCGCCTG AGGAGAAAGC CCAGAATGTC ACACGACTCC TACGAAAGCA GGCTGAGGAG 
GTCAACACAG AGTGGGAAAA ATTGAACCTA CACTCGGCTG ACTGGCAGAG AAAAATAGAC 
GAGGCCCTCG AAAGACTCCA AGAACTGCAG GAGGCCACGG ACGAGCTGGA CCTCAAACTG 
CGCCAAGCTG AGGTGATCAA GGGATCATGG CAGCCCGTGG GCGATCTTCT CATTGACTCC 
CTCCAGGATC ACCTCGAGAA AGTCAAGGCA CTTCGAGGAG AAATTGCACC TCTCAAAGAG 
AATGTAAGCC ACGTCAGTGA CCTTGCTCGC CAACTCACCA CGTTGGGCAT TCAGCTTTCA 
CCATATAATC TCAGCACTCT GGAAGACCTG AACACCAGAT GGAAGCTTCT GCAGGTGGCT 
GTTGAGGACC GCATCAGGCA GCTGCATGAA GCCCACAGGG ACTTTGGCCC AGCGTCCCAG 
CACTTCCTTT CCACTTCTGT CCAGGGTCCC TGGGAGAGAG CCATCTCACC AAACAAAGTG 
CCCTACTATA TCAACCACGA GACCCAAACA ACTTGCTGGG ACCATCCTAA AATGACAGAG 
CTCTACCAGT CTTTAGCTGA CCTGAATAAT GTCAGGTTCT CGGCTTATAG GACTGCCATG 
AAACTCCGAA GACTGCAGAA GGCCCTTTGC TTGGATCTTT TGAGCCTGTC AGCCGCATGT 
GATGCCTTGG ACCAGCACAA CCTCAAGCAA AACGACCAGC CCATGGACAT CCTGCAGATC 
ATTAACTGTT TGACCACTAT TTATGACCGC CTGGAGCAAG AGCACAACAA TCTGGTCAAC 
GTCCCTCTCT GCGTGGATAT GTGTCTCAAT TGGCTGCTGA ATGTTTATGA TACGGGACGA 
ACAGGGAGGA TCCGGGTCCT GTCTTTTAAA ACTGGCATCA TTTCTCTGTG TAAAGCGCAT 
TTGGAAGACA AGTACAGATA CCTTTTCAAG CAAGTGGCCA GTTCAACAGG ATTTTGTGAC 
CAGCGCAGAC TGGGCCTCCT TCTACATGAC TCTATCCAAA TCCCAAGACA GTTGGGTGAA 
GTTGCATCCT TTGGGGGCAG TAACATTGAG CCAAGTGTCA GGAGCTGCTT CCAATTCGCT 
AATAATAAGC CTGAGATCGA AGCAGCTCTC TTCCTCGATT GGATGAGACT AGAACCCCAG 
TCCATGGTGT GGCTGCCCGT CCTGCACAGG GTGGCTGCCG CGGAGACCGC CAAGCATCAG 
GCCAAGTGTA ACATCTGCAA GGAGTGTCCA ATCATCGGCT TCAGGTACAG AAGTCTAAAG 
CACTTTAATT ATGACATCTG CCAAAGCTGC TTTTTCTCTG GCCGCGTTGC AAAAGGCCAT 
AAAATGCACT ATCCCATGGT GGAATATTGT ACTCCGACTA CATCGGGAGA AGATGTTCGA 
GACTTTGCCA AGGTACTAAA AAACAAATTT CGCACCAAAA GGTATTTTGC GAAGCATCCC 
CGAATGGGCT ACCTGCCAGT GCAGACGGTC TTAGAGGGGG ACAACATGGA AACTCCTGTT 
ACTCTGATCA ACTTCTGGCC GGTAGACTCT GCGCCTGCCT CGTCCCCTCA GCTTTCACAC 
GATGATACTC ATTCACGCAT TGAACATTAT GCTAGCAGGC TAGCAGAAAT GGAAAACAGC 
AGTGGATCTT ATCTAAATGA TAGCATCTCT CCTAATGAGA GCATAGATGA TGAACATTTG 
TTAATCCAGC ATTACTGCCA AAGTTTGAAC CAGGACTCCC CCCTGAGCCA GCCTCGTAGT 
CCTGCCCAGA TCTTGATTTC CTTAGAGAGT GAGGAAAGAG GGGAGCTAGA GAGAATCCTA 
GCAGATCTTG AGGAAGAAAA CAGGAATCTG CAGGCTGAAT ATGATCGTCT CAAGGAGCAG 
CATGAACATA AAGGCCTGTC CCCACTGCCG TCCCCTCCTG AGATGATGCC CACCTCGCCA 
CAGAGTCCCC GGGATGCTGA GCTCATTGCT GAGGCCAAGC TACTGCGTCA ACACAAAGGC 
CGCCTGGAAG CCAGGATGCA AATCCTGGAA GACCACAATA AACAGCTGGA GTCCCAGTTA 
CACAGGCTCA GGCAGCTGCT GGAGCAACCC CAGGCTGAGG CCAAGGTGAA TGGCACAACG 
GTGTCCTCTC CTTCTACCTC TCTTCAGAGG TCAGACAGCA GCCAGCCTAT GCTGCTCCGG 
GTGGTTGGCA GTCAAACTTC AGAATCCATG GGCGAGGAAG ACCTTCTCAG TCCTCCCCAG 
GACTCAAGCA CAGGGTTAGA GGAAGTGATG GAGCAACTCA ACAACTCCTT CCCTAGTTCC 
AGAGGACACA ATGTAGGAAG CCTTTTCCAC TTGGCAGATG ATTTGGGCAG AGCGATGGAG 
TCCTTAGTTT CAGTTATGAC AGATGAAGAA GGGGCAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_024988364.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844132.1 Bos taurus dystrophin (DMD), transcript variant X7, mRNA.

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CloneID	OBa238543
Clone ID Related Accession (Same CDS sequence)	XM_024988364.1
Accession Version	XM_024988364.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAATTTTCT AAGTTCGGGA AGCAGCACAT AGAAAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTC TTGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCAGGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAATA TCGGAAGCTC TGACATAGTA 
GATGGAAATC ATAAACTGAC TCTTGGCTTG ATTTGGAATA TAATCCTCCA TTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA GAAGATACTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT CAACTTCACC 
ACCAGCTGGT CTGACGGTTT GGCTTTGAAT GCGCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TCGCCAGCAG TCAGCTACAC AACGACTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTACTGG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TACGTCACAT CTCTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCCTTGAAGC CATCCAGGAG GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACAAGAGAAG AACATTTTCA ACTACATCAT CAAATGCACT ACTCTCAACA GATCACTGTC 
AGTCTAGCAC AGGGCTATGA ACGAACTCCT TCCTCTCCTC AGCCTCGCTT CAAGAGCTAT 
GCCTACACGC AGGCTGCTTA TGTCTCCACC TCTGACCCCA CACGGAGCCC ATTTCCTTCA 
CAGCGTTTGG AAGCTCCTGA AGACAAGTCA TTTGGCAGTC CATTGATGGA GACTGAAGTA 
AACCTGGACA GTTACCAGAC AGCTTTAGAA GAAGTACTCT CATGGCTCCT TTCAGCCGAA 
GACACATTGC AAGCACAAGG AGAGATTTCT AATGATGTAG AAGAAGTGAA AGAACAGTTT 
CATACCCACG AGGGATACAT GATGGATTTG ACATCCCATC AGGGACGGGT TGGCAATGTT 
CTCCAATTGG GGAGTCAGCT GATTGGGTCA GGGAAATTAT CAGAAGATGA AGAAACTGAA 
GTGCAAGAAC AAATGAATCT TCTAAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTC ATGGATCTCC AGAATCAGCA GCTGAAAGAG 
TTAAATGCCT GGTTAACAAA AACAGAAGAG AGAACAAGGA AAATGGAAAA AGAGCCCCTT 
GGACCTGATC TTGAGGACCT CAAACGCCAA ATACAGCAAC ATAAGGTGCT TCAAGAAGAC 
CTAGAACAGG AACAAGTCAG AGTCAATTCT CTCACGCACA TGGTGGTGGT TGTAGATGAG 
TCTAGTGGTG ATCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGG 
TGGGCAAACA TCTGTAGGTG GACTGAAGAT CGCTGGGTTC TTTTACAGGA TGTTCTTCTA 
AAATGGCAAC GTTTTACTGA AGAGCAGTGC CTTTTTAGTA CATGGCTTTC AGATAAAGAA 
GATGCATTGA ACAAGATTCC CACAAGTGGC TTTAAGGATC AAAGTGAAAT GTTATCAAGT 
CTTCAAAAAC TGGCCGTTTT AAAAACTGAT CTAGAAAAGA AAAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCATGA TCTCCTTTCA ACACTGAAAA ATACATTGGT TGCCCAAAAG 
ATGGAAGCAT GGTTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA AAAACTTGAA 
AAGAGTTCAA CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACGGTAAC TATGGTGACC ACGAGGGAGC AAATCCTGGT AAAGCATGCC 
CAAGAGGAAC TCCCTCCACC ACCTCCACAA AAGAAGAGGC AGATTATTGT GGATTCTGAA 
ATTAAGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCCGTGTTAC AGAGTCCTGA ATTTGCAGTC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGTATCAAAC AAGCCTCAGA ACAACTGAAC AGCCGGTGGA TCGAGTTCTG CCAATTGCTA 
AGTGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCA GAAAACTGGT TGAAAACTCA ACCCACTACC 
CCATCAGAGC CAACAGCAGT TAAAAGCCAG TTAAAAAATT GTAAGGATGA AGTCAACCGG 
CTGTCAGCTC TTCAGCCTCA AATCGAGAGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCC GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATATGCA GGCCAGAGAG AAAGAGTTAC AGACAATTTT GGACACTTTA 
CCCACCGTGC GGTATCAGGA GACCATGAGT ACCATCCTCA CGTGGATCCA ACAGTCAGAA 
ACCAAACTCT CGATACCTCA GGTTACTGTC ACTGAATACG AGATCATGGA GCAGAGACTT 
GAGGAGTTAC AGGCTTTACA AAGCTCTCTG CAAGAGCACC AAAATGACCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAGGAAA GCACCCTCTC ATATAAGCCA GAGATATCAG 
TCAGAATTTG AAAACATTGA GGGTCGGTGG AAGAAACTCT CTGCCCAGCT GAATGAACGT 
TGCCAAAAGC TAGAGGAGCA AATGGCCAAA CTCCGAAAAC TTCAGAATCA CATAAAAACA 
CTGAAGAAGT GGATGGCTGA AGTTGATGTT TTCTTGAAAG ATGAATGGCC TGCCCTTGGA 
GATTCAGAAA TTCTTAAAAA ACAGCTGAAG CAGTGCAGGC TTTTAGTCAG TGATATTCAG 
ACAATCCAGC CCAGTCTAAA CAGTGTCAAT GAAGGTGGAC AGAAAATAAA AACTGAAGCG 
GAGCCAGAGT TTGCTTCTAG ACTGGAGACA GAGCTCAGAG AACTTAACAC TCAATGGGAT 
TACATATGTC GACAGGTCTA TGCCAGAAAG GAGGCCTTAA AGGGAGGCTT GGATAAAACT 
GTAAGCCTTC AGAAAGATTT GTCAGAGATG CATGAATGGA TGACACAAGC CGAAGAAGAA 
TACCTAGAGA GAGATTTTGA ATATAAAACC CCAGATGAAT TACAGACAGC AGTTGAAGAG 
ATGAAGAGAG CTAAAGAAGA GGCCCAGCAA AAAGAAGCAA AAGTGCAGCT CCTCACTGAG 
TCCGTGAACA GTGTCATAGC TCAAGCTCCA CCTGCAGCAC AAGAGGCCTT AAGAAAGGAA 
CTGGACACTC TGACCACCAA CTACCAGTGG CTCTGCACCA GGCTGAATGG AAAATGCAAG 
ACGTTAGAAG AGGTTTGGGC ATGTTGGCAT GAGTTATTGT CATACTTGGA AAAGGCAAAT 
AAGTGGCTAA ATGAAGTAGA ATTGAAACTT AAGACTACTG AAAATATTCC TGGTGGAGCT 
GAGGAAATCT CTGAGGTGCT CAGTTCACTT GAAAATTTGA TGCAACATTC AGAAGATAAC 
CCGAATCAGA TTCGCATATT GGCACAGACC CTAACAGATG GTGGAGTCAT GGATGAACTG 
ATCAATGAAG AACTCGAGAC ATTTAATTCT CGTTGGAGAG AACTACATGA AGAGGCTGTG 
AGGAGGCAAA AGTTGCTTGA ACAGAGTATC CAGTCTGCCC AGGAGATTGA AAAATCCTTA 
CAGTTAATTC AGGAATCCCT CTCATCAATT GACAAGCAGT TGGCAGCTTA TATCGCTGAC 
AAAGTGGACG CCGCTCAGAT GCCTCAGGAA GCCCAGAAAA TCCAATCTGA TTTGACAAGT 
CATGAGATCA GTTTAGAAGA AATGAAGAAA CATTACCAAG GAAAGGAAAC TGCTCAACGG 
GTACTATCCC AAATTGAAGT GGCACAGAAA AAAATGCAAG ATGTTTCCAT GAAATTTCGA 
TTGTTCCAGA AGCCAGCCAA TTTTGAGCAG CGTCTACAGG AAAGTAAGAT GATTTTAGAT 
GAAGTGAAGA TGCACTTGCC CGCACTGGAA ACAAAGAGTG TTGAACAGGA AGTAGTACAG 
TCACAGTTAA ATCATTGTGT GAACTTGTAT AAAAGTCTGA GTGAAGTGAA GTCTGAAGTG 
GAAATGGTGA TTAAAACTGG ACGTCAAATT GTACAGAAGA AGCAGACGGA AAACCCTAAA 
GAGCTTGATG AAAGAGTAAC GGCTTTGAAA TTGCATTATA ATGAGCTGGG AGCAAAGGTA 
ACAGAAAGAA AGCAACAGTT GGAGAAATGC TTGAAATTGT CCCGTAAGAT GCGAAAGGAA 
ATGAATGCCT TGACAGAATG GCTGGCAGCT ACAGATTTGG AACTGACAAA GAGATCAGCA 
GTTGAAGGAA TGCCTAGTAA TTTGGATTCT GAAGTTGCCT GGGGAAAGGC TACTCAGAAA 
GAGACTGAGA AACAGAAGGT TCACCTAAAG AGCATTACAG AGCTAGGAGA GGCCTTGAAA 
ACGGTTTTGG GCAAGAAGGA GACCTTGGTC GAAGATAAAC TGAGTCTTTT GAATAGTAAC 
TGGATAGCCG TCACTTCTCG AGCAGAAGAG TGGCTAAACC TTTTGTTGGA ATACCAGAAA 
CACATGGAAA CCTTTGACCA GAATGTGGAT CATATCACAA AGTGGATCAT TCAAGCTGAT 
GCACTTCTGG ATGAATCTGA GAAAAAGAAA CCACAGCAAA AAGAAGACAT GCTAAAGCGC 
TTAAAGGCTG AACTGAATGA CATACGCCCA AAGGTGGACT CCACGCGTGA CCAAGCAGCA 
AACTTGATGG CAAACCGCGG CGACCACTGC AGGAAAGTAA TAGAGCCCAA AATCTCAGAG 
CTCAACCATC GATTTGCAGC CATTTCTCAC AGAATTAAGA CTGGAAAGGC CTCCATTCCT 
TTGAAGGAAT TGGAGCAGTT TAACTCAGAT ATACAAAAAT TGCTTGAACC ACTGGAGGCT 
GAAATTCAGC AGGGGGTGAA TCTGAAAGAG GAAGACTTCA ATAAAGATAT GGATTTGAGG 
TCTCAAAGAA GAAAAAAGGC TCTAGAAATT TCTCACCAGT GGTATCAGTA CAAGAGGCAG 
GCTGATGATC TCCTGAAATG CTTGGATGAC ATTGAAAAAA AATTAGCCAG CCTACCTGAA 
CCCAGAGATG AAAGGAAAAT AAAGGAAATT GATCGTGAGT TGCAGAAGAA GAAAGAGGAG 
CTGAATGCAG TGCATAGGCA AGCTGAGGGC TTGTCTGAGG ATGGGGCAGC AATGGCAGTG 
GAGTCAACTC AGATCCAGCT CAGCAAGCGC TGGCGGGAAA TTGAGAGCAA ATTTGCTCAG 
TTTCGAAGAC TCAACTTTGC ACAAATTCAC ACTGTCCATG AAGAATCAGT GATGGTGATG 
ACCGAAGACA TGCCTTTGGA AATTTCTTAT GTGCCTTCTA CTTACCTGAC TGAGATCACT 
CACGTCTTAC AAGCCCTTTC AGAAGTGGAA CAACTTCTCA ATGCTCCCGA CCTCTGTGCT 
AAAGATTTTC AAGATCTCTT CAAACAAGAG GAATCTCTGA AGAATATAAA AGACAACCTG 
CAACAAATCT CAGGTCGGAT TGATGTTATT CACAATAAGA AGGCGGCAGC ATTGCAAAGC 
ACCACACCCC CAGAAAAGGC AAGGTTACAG GAAGCTCTCT CCAGGCTCGA CTTCCAATGG 
GAAAGAGTGA ACAAAATGTA CAAGGACCGA CAAGGGCAAT TTGACAGATC TGTTGAAAAA 
TGGCGACGGT TTCATTATGA TATGAAGATA TTTAATCAAT GGCTAACAGA AGCTGAACAC 
TTTCTCAAAA AGACACAAAT TCCTGAGAAC TGGGAACATG CCAAATATAA ATGGTATCTT 
AAGGAACTCC AGGATGGCAT CGGGCAGCGG CAAACTGTTG TCAGAGTATT GAATGCAACT 
GGGGAAGAAA TAATTCAGCA GTCCTCAAAA ATAGATGCCA GTATTCTACA AGAAAAATTG 
GGAAGCCTGA ATCTGCGGTG GCAGGAGGTC TGCAAACAGC TGGCCGAGAG AAAAAAGAGG 
TTAGAAGAAC AAAAGAATAT CCTGTCAGAA TTTCAAAGAG ATTTGAATGA ATTTGTTTTA 
TGGTTGGAAG AAGCAGGTAA CATTTCTAGT ATTCCTCTTG AACCTGGAAA TGAGCAGCAA 
CTAAAAGAAA AGCTTGAGGA AGTCAAGTTA CTGGCCGAAG AGTTGCCCCT GCGTCAGGGA 
ATTCTAAAAC AATTAAATGA AACAGGAGGA ACAGTGCTTG TAAGTGCTCC CATAAGCCCA 
GAAGAGCAAG ATAAACTTGA AAATAAGCTC AAGCAGACAA ATCTTCAGTG GATAAAGGTT 
TCTAGGAGTT TACCTGAGAA ACAGGGAGAA ATTGAGGCTC ACATAAAAGA CCTTGGACAG 
CTTGAAGAGC AATTAAATCA TCTGCTTCTG TGGCTGTCTC CTATTAGGAG TCAGTTGGAA 
ATTTACAACC AACCAAATCA GACAGGACCA TTTGACATTA AGGAAACTGA AGTAGCAGTT 
CAAGCTAAAC AGCCGGATGT GGAAGGGATT TTGTCTAAAG GGCAGAATTT GTACAAGGAA 
AAACCAGCCA CTGAGCCAGT GAAGAGGAAG TTAGAAGATC TGAGCTCGGA GTGGAAGGCG 
GTAACCCACT TACTTCAAGA GCTGAGGGCA AAGTGGCCTG GCCCAGTCCC TGGACTGACA 
GCAACTGGAG CACCTCCTAG TCAGACGGTT GCTCTGGTGA CACAACCTGT GGTTGCCAAG 
GAAACCGCTG TCTCCAAACT AGAAATGCCA TCTTCCTTGC TGTTGGAGGT CCCTGCACTG 
GCAGATTTCA ACCGGGCTTG GACAGAACTT ACCGACTGGC TGTCTCTGCT TGATCGAGTT 
CTGAAAGCAC AGAGGGTGAT GGTGGGTGAT CTTGAAGACA TCAACGAGAT GATCATCAAG 
CAGAAGGCAA CACTGCAGGA CTTGGAGCAG AGGCGCCCCC AGTTGGAAGA ACTCATTACT 
GCTGCCCAGA ATTTGAAAAA CAAGACCAGC AATCAAGAGG CCAGGACCAT CATTACTGAT 
CGAATTGAAA GAATTCAGAG TCAGTGGGAT GAAGTACAGG AACACCTTCA GAACCGGAGG 
CAGCAGTTAA ATGAAATGTT AAAGGATTCA ACACAATGGC TGGAAGCTAA GGAAGAAGCT 
GAGCAGGTGG TGGGGCAGGC CAGAGCCAAG CTTGAGACAT GGAAGGAGGG TCCCTACACA 
ATGGATGCAA TCCAAAGGAA GATCACAGAA ACCAAGCAGT TGGCCAAAGA CCTCCGCCAG 
TGGCAGATAA ATGTAGATGT GGCAAATGAC TTGGCACTGA AACTTCTCCG GGATTATTCT 
GCAGATGATA CCCGAAAGGT ACACATGATA ACAGAGAACA TCAATGCCTC TTGGGCAAGC 
ATTCATAAAA GAGTGAGTGA GCGAGAGACT GCTCTGGAAG AAACTCATAG ATTACTGCAA 
CAGTTCCCCC TGGACTTGGA GAAGTTTCTT GCCTGGCTTA CAGAAGCTGA AACAACTGCC 
AACGTCCTGC AGGATGCCAC CCATAAGGAA AGGCTTCTAG AAGACTCCAA GGGAGTAAGA 
GAGCTGATGA AACAATGGCA GGACCTCCAA GGAGAAATTG AAGCTCACAC AGATATCTAT 
CACAACCTAG ATGAAAATGG CCAAAAAATC CTGAGATCCC TGGAAGGTTC TGATGACGCG 
GTCCTATTAC AAAGACGTTT GGATAATATG AATTTCAAGT GGAGTGAGCT GCGGAAAAAG 
TCTCTCAATA TCAGGTCCCA TTTGGAAGCC AGTTCTGACC AGTGGAAGCG TCTGCACCTT 
TCTCTTCAGG AACTTCTGGT GTGGCTACAG CTGAAAGACG ATGAGTTGAG CCGTCAGGCA 
CCTATTGGAG GCGATTTCCC AGCAGTTCAG AAGCAGAATG ATATACACAG GGCCTTCAAG 
AGGGAACTGA AAACGAAAGA ACCTGTAATC ATGAGTACTC TTGAGACTGT ACGAATATTT 
CTGACAGAGC AGCCTTTGGA AGGACTAGAG AAACTCTACC AGGAGCCCAG AGAGCTGCCG 
CCTGAGGAGA AAGCCCAGAA TGTCACACGA CTCCTACGAA AGCAGGCTGA GGAGGTCAAC 
ACAGAGTGGG AAAAATTGAA CCTACACTCG GCTGACTGGC AGAGAAAAAT AGACGAGGCC 
CTCGAAAGAC TCCAAGAACT GCAGGAGGCC ACGGACGAGC TGGACCTCAA ACTGCGCCAA 
GCTGAGGTGA TCAAGGGATC ATGGCAGCCC GTGGGCGATC TTCTCATTGA CTCCCTCCAG 
GATCACCTCG AGAAAGTCAA GGCACTTCGA GGAGAAATTG CACCTCTCAA AGAGAATGTA 
AGCCACGTCA GTGACCTTGC TCGCCAACTC ACCACGTTGG GCATTCAGCT TTCACCATAT 
AATCTCAGCA CTCTGGAAGA CCTGAACACC AGATGGAAGC TTCTGCAGGT GGCTGTTGAG 
GACCGCATCA GGCAGCTGCA TGAAGCCCAC AGGGACTTTG GCCCAGCGTC CCAGCACTTC 
CTTTCCACTT CTGTCCAGGG TCCCTGGGAG AGAGCCATCT CACCAAACAA AGTGCCCTAC 
TATATCAACC ACGAGACCCA AACAACTTGC TGGGACCATC CTAAAATGAC AGAGCTCTAC 
CAGTCTTTAG CTGACCTGAA TAATGTCAGG TTCTCGGCTT ATAGGACTGC CATGAAACTC 
CGAAGACTGC AGAAGGCCCT TTGCTTGGAT CTTTTGAGCC TGTCAGCCGC ATGTGATGCC 
TTGGACCAGC ACAACCTCAA GCAAAACGAC CAGCCCATGG ACATCCTGCA GATCATTAAC 
TGTTTGACCA CTATTTATGA CCGCCTGGAG CAAGAGCACA ACAATCTGGT CAACGTCCCT 
CTCTGCGTGG ATATGTGTCT CAATTGGCTG CTGAATGTTT ATGATACGGG ACGAACAGGG 
AGGATCCGGG TCCTGTCTTT TAAAACTGGC ATCATTTCTC TGTGTAAAGC GCATTTGGAA 
GACAAGTACA GATACCTTTT CAAGCAAGTG GCCAGTTCAA CAGGATTTTG TGACCAGCGC 
AGACTGGGCC TCCTTCTACA TGACTCTATC CAAATCCCAA GACAGTTGGG TGAAGTTGCA 
TCCTTTGGGG GCAGTAACAT TGAGCCAAGT GTCAGGAGCT GCTTCCAATT CGCTAATAAT 
AAGCCTGAGA TCGAAGCAGC TCTCTTCCTC GATTGGATGA GACTAGAACC CCAGTCCATG 
GTGTGGCTGC CCGTCCTGCA CAGGGTGGCT GCCGCGGAGA CCGCCAAGCA TCAGGCCAAG 
TGTAACATCT GCAAGGAGTG TCCAATCATC GGCTTCAGGT ACAGAAGTCT AAAGCACTTT 
AATTATGACA TCTGCCAAAG CTGCTTTTTC TCTGGCCGCG TTGCAAAAGG CCATAAAATG 
CACTATCCCA TGGTGGAATA TTGTACTCCG ACTACATCGG GAGAAGATGT TCGAGACTTT 
GCCAAGGTAC TAAAAAACAA ATTTCGCACC AAAAGGTATT TTGCGAAGCA TCCCCGAATG 
GGCTACCTGC CAGTGCAGAC GGTCTTAGAG GGGGACAACA TGGAAACTCC TGTTACTCTG 
ATCAACTTCT GGCCGGTAGA CTCTGCGCCT GCCTCGTCCC CTCAGCTTTC ACACGATGAT 
ACTCATTCAC GCATTGAACA TTATGCTAGC AGGCTAGCAG AAATGGAAAA CAGCAGTGGA 
TCTTATCTAA ATGATAGCAT CTCTCCTAAT GAGAGCATAG ATGATGAACA TTTGTTAATC 
CAGCATTACT GCCAAAGTTT GAACCAGGAC TCCCCCCTGA GCCAGCCTCG TAGTCCTGCC 
CAGATCTTGA TTTCCTTAGA GAGTGAGGAA AGAGGGGAGC TAGAGAGAAT CCTAGCAGAT 
CTTGAGGAAG AAAACAGGAA TCTGCAGGCT GAATATGATC GTCTCAAGGA GCAGCATGAA 
CATAAAGGCC TGTCCCCACT GCCGTCCCCT CCTGAGATGA TGCCCACCTC GCCACAGAGT 
CCCCGGGATG CTGAGCTCAT TGCTGAGGCC AAGCTACTGC GTCAACACAA AGGCCGCCTG 
GAAGCCAGGA TGCAAATCCT GGAAGACCAC AATAAACAGC TGGAGTCCCA GTTACACAGG 
CTCAGGCAGC TGCTGGAGCA ACCCCAGGCT GAGGCCAAGG TGAATGGCAC AACGGTGTCC 
TCTCCTTCTA CCTCTCTTCA GAGGTCAGAC AGCAGCCAGC CTATGCTGCT CCGGGTGGTT 
GGCAGTCAAA CTTCAGAATC CATGGGCGAG GAAGACCTTC TCAGTCCTCC CCAGGACTCA 
AGCACAGGGT TAGAGGAAGT GATGGAGCAA CTCAACAACT CCTTCCCTAG TTCCAGAGGA 
CACAATGTAG GAAGCCTTTT CCACTTGGCA GATGATTTGG GCAGAGCGAT GGAGTCCTTA 
GTTTCAGTTA TGACAGATGA AGAAGGGGCA GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844132.1
CDS	251..11206
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALQVLQKNNVDLVNIGSSDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVRQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYVTSLFQVLPQQVSLEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERTPSSPQPRFKSYAYTQAAYVSTSDPTRSPFPSQRLEAPEDKSFGSPLMETEVNLDSYQTALEEVLSWLLSAEDTLQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGSGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNAWLTKTEERTRKMEKEPLGPDLEDLKRQIQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDVLLKWQRFTEEQCLFSTWLSDKEDALNKIPTSGFKDQSEMLSSLQKLAVLKTDLEKKKQTMDKLCSLNHDLLSTLKNTLVAQKMEAWLDNFAQRWDNLVQKLEKSSTQISQAVTTTQPSLTQTTVMETVTMVTTREQILVKHAQEELPPPPPQKKRQIIVDSEIKKRLDVDITELHSWITRSEAVLQSPEFAVYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTPSEPTAVKSQLKNCKDEVNRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADMQAREKELQTILDTLPTVRYQETMSTILTWIQQSETKLSIPQVTVTEYEIMEQRLEELQALQSSLQEHQNDLNYLSTTVKEMSRKAPSHISQRYQSEFENIEGRWKKLSAQLNERCQKLEEQMAKLRKLQNHIKTLKKWMAEVDVFLKDEWPALGDSEILKKQLKQCRLLVSDIQTIQPSLNSVNEGGQKIKTEAEPEFASRLETELRELNTQWDYICRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVQLLTESVNSVIAQAPPAAQEALRKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLNEVELKLKTTENIPGGAEEISEVLSSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLQLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHYQGKETAQRVLSQIEVAQKKMQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDLELTKRSAVEGMPSNLDSEVAWGKATQKETEKQKVHLKSITELGEALKTVLGKKETLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMETFDQNVDHITKWIIQADALLDESEKKKPQQKEDMLKRLKAELNDIRPKVDSTRDQAANLMANRGDHCRKVIEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVHRQAEGLSEDGAAMAVESTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVMVMTEDMPLEISYVPSTYLTEITHVLQALSEVEQLLNAPDLCAKDFQDLFKQEESLKNIKDNLQQISGRIDVIHNKKAAALQSTTPPEKARLQEALSRLDFQWERVNKMYKDRQGQFDRSVEKWRRFHYDMKIFNQWLTEAEHFLKKTQIPENWEHAKYKWYLKELQDGIGQRQTVVRVLNATGEEIIQQSSKIDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDLNEFVLWLEEAGNISSIPLEPGNEQQLKEKLEEVKLLAEELPLRQGILKQLNETGGTVLVSAPISPEEQDKLENKLKQTNLQWIKVSRSLPEKQGEIEAHIKDLGQLEEQLNHLLLWLSPIRSQLEIYNQPNQTGPFDIKETEVAVQAKQPDVEGILSKGQNLYKEKPATEPVKRKLEDLSSEWKAVTHLLQELRAKWPGPVPGLTATGAPPSQTVALVTQPVVAKETAVSKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVLKAQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVVGQARAKLETWKEGPYTMDAIQRKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRVSERETALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKILRSLEGSDDAVLLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDIHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEEKAQNVTRLLRKQAEEVNTEWEKLNLHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVSHVSDLARQLTTLGIQLSPYNLSTLEDLNTRWKLLQVAVEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSSGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKEQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDSSTGLEEVMEQLNNSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_024988364.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophin (DMD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988364.1

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAATTTTCT AAGTTCGGGA AGCAGCACAT AGAAAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTC TTGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCAGGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAATA TCGGAAGCTC TGACATAGTA 
GATGGAAATC ATAAACTGAC TCTTGGCTTG ATTTGGAATA TAATCCTCCA TTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA GAAGATACTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT CAACTTCACC 
ACCAGCTGGT CTGACGGTTT GGCTTTGAAT GCGCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TCGCCAGCAG TCAGCTACAC AACGACTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTACTGG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TACGTCACAT CTCTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCCTTGAAGC CATCCAGGAG GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACAAGAGAAG AACATTTTCA ACTACATCAT CAAATGCACT ACTCTCAACA GATCACTGTC 
AGTCTAGCAC AGGGCTATGA ACGAACTCCT TCCTCTCCTC AGCCTCGCTT CAAGAGCTAT 
GCCTACACGC AGGCTGCTTA TGTCTCCACC TCTGACCCCA CACGGAGCCC ATTTCCTTCA 
CAGCGTTTGG AAGCTCCTGA AGACAAGTCA TTTGGCAGTC CATTGATGGA GACTGAAGTA 
AACCTGGACA GTTACCAGAC AGCTTTAGAA GAAGTACTCT CATGGCTCCT TTCAGCCGAA 
GACACATTGC AAGCACAAGG AGAGATTTCT AATGATGTAG AAGAAGTGAA AGAACAGTTT 
CATACCCACG AGGGATACAT GATGGATTTG ACATCCCATC AGGGACGGGT TGGCAATGTT 
CTCCAATTGG GGAGTCAGCT GATTGGGTCA GGGAAATTAT CAGAAGATGA AGAAACTGAA 
GTGCAAGAAC AAATGAATCT TCTAAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTC ATGGATCTCC AGAATCAGCA GCTGAAAGAG 
TTAAATGCCT GGTTAACAAA AACAGAAGAG AGAACAAGGA AAATGGAAAA AGAGCCCCTT 
GGACCTGATC TTGAGGACCT CAAACGCCAA ATACAGCAAC ATAAGGTGCT TCAAGAAGAC 
CTAGAACAGG AACAAGTCAG AGTCAATTCT CTCACGCACA TGGTGGTGGT TGTAGATGAG 
TCTAGTGGTG ATCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGG 
TGGGCAAACA TCTGTAGGTG GACTGAAGAT CGCTGGGTTC TTTTACAGGA TGTTCTTCTA 
AAATGGCAAC GTTTTACTGA AGAGCAGTGC CTTTTTAGTA CATGGCTTTC AGATAAAGAA 
GATGCATTGA ACAAGATTCC CACAAGTGGC TTTAAGGATC AAAGTGAAAT GTTATCAAGT 
CTTCAAAAAC TGGCCGTTTT AAAAACTGAT CTAGAAAAGA AAAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCATGA TCTCCTTTCA ACACTGAAAA ATACATTGGT TGCCCAAAAG 
ATGGAAGCAT GGTTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA AAAACTTGAA 
AAGAGTTCAA CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACGGTAAC TATGGTGACC ACGAGGGAGC AAATCCTGGT AAAGCATGCC 
CAAGAGGAAC TCCCTCCACC ACCTCCACAA AAGAAGAGGC AGATTATTGT GGATTCTGAA 
ATTAAGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCCGTGTTAC AGAGTCCTGA ATTTGCAGTC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGTATCAAAC AAGCCTCAGA ACAACTGAAC AGCCGGTGGA TCGAGTTCTG CCAATTGCTA 
AGTGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCA GAAAACTGGT TGAAAACTCA ACCCACTACC 
CCATCAGAGC CAACAGCAGT TAAAAGCCAG TTAAAAAATT GTAAGGATGA AGTCAACCGG 
CTGTCAGCTC TTCAGCCTCA AATCGAGAGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCC GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATATGCA GGCCAGAGAG AAAGAGTTAC AGACAATTTT GGACACTTTA 
CCCACCGTGC GGTATCAGGA GACCATGAGT ACCATCCTCA CGTGGATCCA ACAGTCAGAA 
ACCAAACTCT CGATACCTCA GGTTACTGTC ACTGAATACG AGATCATGGA GCAGAGACTT 
GAGGAGTTAC AGGCTTTACA AAGCTCTCTG CAAGAGCACC AAAATGACCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAGGAAA GCACCCTCTC ATATAAGCCA GAGATATCAG 
TCAGAATTTG AAAACATTGA GGGTCGGTGG AAGAAACTCT CTGCCCAGCT GAATGAACGT 
TGCCAAAAGC TAGAGGAGCA AATGGCCAAA CTCCGAAAAC TTCAGAATCA CATAAAAACA 
CTGAAGAAGT GGATGGCTGA AGTTGATGTT TTCTTGAAAG ATGAATGGCC TGCCCTTGGA 
GATTCAGAAA TTCTTAAAAA ACAGCTGAAG CAGTGCAGGC TTTTAGTCAG TGATATTCAG 
ACAATCCAGC CCAGTCTAAA CAGTGTCAAT GAAGGTGGAC AGAAAATAAA AACTGAAGCG 
GAGCCAGAGT TTGCTTCTAG ACTGGAGACA GAGCTCAGAG AACTTAACAC TCAATGGGAT 
TACATATGTC GACAGGTCTA TGCCAGAAAG GAGGCCTTAA AGGGAGGCTT GGATAAAACT 
GTAAGCCTTC AGAAAGATTT GTCAGAGATG CATGAATGGA TGACACAAGC CGAAGAAGAA 
TACCTAGAGA GAGATTTTGA ATATAAAACC CCAGATGAAT TACAGACAGC AGTTGAAGAG 
ATGAAGAGAG CTAAAGAAGA GGCCCAGCAA AAAGAAGCAA AAGTGCAGCT CCTCACTGAG 
TCCGTGAACA GTGTCATAGC TCAAGCTCCA CCTGCAGCAC AAGAGGCCTT AAGAAAGGAA 
CTGGACACTC TGACCACCAA CTACCAGTGG CTCTGCACCA GGCTGAATGG AAAATGCAAG 
ACGTTAGAAG AGGTTTGGGC ATGTTGGCAT GAGTTATTGT CATACTTGGA AAAGGCAAAT 
AAGTGGCTAA ATGAAGTAGA ATTGAAACTT AAGACTACTG AAAATATTCC TGGTGGAGCT 
GAGGAAATCT CTGAGGTGCT CAGTTCACTT GAAAATTTGA TGCAACATTC AGAAGATAAC 
CCGAATCAGA TTCGCATATT GGCACAGACC CTAACAGATG GTGGAGTCAT GGATGAACTG 
ATCAATGAAG AACTCGAGAC ATTTAATTCT CGTTGGAGAG AACTACATGA AGAGGCTGTG 
AGGAGGCAAA AGTTGCTTGA ACAGAGTATC CAGTCTGCCC AGGAGATTGA AAAATCCTTA 
CAGTTAATTC AGGAATCCCT CTCATCAATT GACAAGCAGT TGGCAGCTTA TATCGCTGAC 
AAAGTGGACG CCGCTCAGAT GCCTCAGGAA GCCCAGAAAA TCCAATCTGA TTTGACAAGT 
CATGAGATCA GTTTAGAAGA AATGAAGAAA CATTACCAAG GAAAGGAAAC TGCTCAACGG 
GTACTATCCC AAATTGAAGT GGCACAGAAA AAAATGCAAG ATGTTTCCAT GAAATTTCGA 
TTGTTCCAGA AGCCAGCCAA TTTTGAGCAG CGTCTACAGG AAAGTAAGAT GATTTTAGAT 
GAAGTGAAGA TGCACTTGCC CGCACTGGAA ACAAAGAGTG TTGAACAGGA AGTAGTACAG 
TCACAGTTAA ATCATTGTGT GAACTTGTAT AAAAGTCTGA GTGAAGTGAA GTCTGAAGTG 
GAAATGGTGA TTAAAACTGG ACGTCAAATT GTACAGAAGA AGCAGACGGA AAACCCTAAA 
GAGCTTGATG AAAGAGTAAC GGCTTTGAAA TTGCATTATA ATGAGCTGGG AGCAAAGGTA 
ACAGAAAGAA AGCAACAGTT GGAGAAATGC TTGAAATTGT CCCGTAAGAT GCGAAAGGAA 
ATGAATGCCT TGACAGAATG GCTGGCAGCT ACAGATTTGG AACTGACAAA GAGATCAGCA 
GTTGAAGGAA TGCCTAGTAA TTTGGATTCT GAAGTTGCCT GGGGAAAGGC TACTCAGAAA 
GAGACTGAGA AACAGAAGGT TCACCTAAAG AGCATTACAG AGCTAGGAGA GGCCTTGAAA 
ACGGTTTTGG GCAAGAAGGA GACCTTGGTC GAAGATAAAC TGAGTCTTTT GAATAGTAAC 
TGGATAGCCG TCACTTCTCG AGCAGAAGAG TGGCTAAACC TTTTGTTGGA ATACCAGAAA 
CACATGGAAA CCTTTGACCA GAATGTGGAT CATATCACAA AGTGGATCAT TCAAGCTGAT 
GCACTTCTGG ATGAATCTGA GAAAAAGAAA CCACAGCAAA AAGAAGACAT GCTAAAGCGC 
TTAAAGGCTG AACTGAATGA CATACGCCCA AAGGTGGACT CCACGCGTGA CCAAGCAGCA 
AACTTGATGG CAAACCGCGG CGACCACTGC AGGAAAGTAA TAGAGCCCAA AATCTCAGAG 
CTCAACCATC GATTTGCAGC CATTTCTCAC AGAATTAAGA CTGGAAAGGC CTCCATTCCT 
TTGAAGGAAT TGGAGCAGTT TAACTCAGAT ATACAAAAAT TGCTTGAACC ACTGGAGGCT 
GAAATTCAGC AGGGGGTGAA TCTGAAAGAG GAAGACTTCA ATAAAGATAT GGATTTGAGG 
TCTCAAAGAA GAAAAAAGGC TCTAGAAATT TCTCACCAGT GGTATCAGTA CAAGAGGCAG 
GCTGATGATC TCCTGAAATG CTTGGATGAC ATTGAAAAAA AATTAGCCAG CCTACCTGAA 
CCCAGAGATG AAAGGAAAAT AAAGGAAATT GATCGTGAGT TGCAGAAGAA GAAAGAGGAG 
CTGAATGCAG TGCATAGGCA AGCTGAGGGC TTGTCTGAGG ATGGGGCAGC AATGGCAGTG 
GAGTCAACTC AGATCCAGCT CAGCAAGCGC TGGCGGGAAA TTGAGAGCAA ATTTGCTCAG 
TTTCGAAGAC TCAACTTTGC ACAAATTCAC ACTGTCCATG AAGAATCAGT GATGGTGATG 
ACCGAAGACA TGCCTTTGGA AATTTCTTAT GTGCCTTCTA CTTACCTGAC TGAGATCACT 
CACGTCTTAC AAGCCCTTTC AGAAGTGGAA CAACTTCTCA ATGCTCCCGA CCTCTGTGCT 
AAAGATTTTC AAGATCTCTT CAAACAAGAG GAATCTCTGA AGAATATAAA AGACAACCTG 
CAACAAATCT CAGGTCGGAT TGATGTTATT CACAATAAGA AGGCGGCAGC ATTGCAAAGC 
ACCACACCCC CAGAAAAGGC AAGGTTACAG GAAGCTCTCT CCAGGCTCGA CTTCCAATGG 
GAAAGAGTGA ACAAAATGTA CAAGGACCGA CAAGGGCAAT TTGACAGATC TGTTGAAAAA 
TGGCGACGGT TTCATTATGA TATGAAGATA TTTAATCAAT GGCTAACAGA AGCTGAACAC 
TTTCTCAAAA AGACACAAAT TCCTGAGAAC TGGGAACATG CCAAATATAA ATGGTATCTT 
AAGGAACTCC AGGATGGCAT CGGGCAGCGG CAAACTGTTG TCAGAGTATT GAATGCAACT 
GGGGAAGAAA TAATTCAGCA GTCCTCAAAA ATAGATGCCA GTATTCTACA AGAAAAATTG 
GGAAGCCTGA ATCTGCGGTG GCAGGAGGTC TGCAAACAGC TGGCCGAGAG AAAAAAGAGG 
TTAGAAGAAC AAAAGAATAT CCTGTCAGAA TTTCAAAGAG ATTTGAATGA ATTTGTTTTA 
TGGTTGGAAG AAGCAGGTAA CATTTCTAGT ATTCCTCTTG AACCTGGAAA TGAGCAGCAA 
CTAAAAGAAA AGCTTGAGGA AGTCAAGTTA CTGGCCGAAG AGTTGCCCCT GCGTCAGGGA 
ATTCTAAAAC AATTAAATGA AACAGGAGGA ACAGTGCTTG TAAGTGCTCC CATAAGCCCA 
GAAGAGCAAG ATAAACTTGA AAATAAGCTC AAGCAGACAA ATCTTCAGTG GATAAAGGTT 
TCTAGGAGTT TACCTGAGAA ACAGGGAGAA ATTGAGGCTC ACATAAAAGA CCTTGGACAG 
CTTGAAGAGC AATTAAATCA TCTGCTTCTG TGGCTGTCTC CTATTAGGAG TCAGTTGGAA 
ATTTACAACC AACCAAATCA GACAGGACCA TTTGACATTA AGGAAACTGA AGTAGCAGTT 
CAAGCTAAAC AGCCGGATGT GGAAGGGATT TTGTCTAAAG GGCAGAATTT GTACAAGGAA 
AAACCAGCCA CTGAGCCAGT GAAGAGGAAG TTAGAAGATC TGAGCTCGGA GTGGAAGGCG 
GTAACCCACT TACTTCAAGA GCTGAGGGCA AAGTGGCCTG GCCCAGTCCC TGGACTGACA 
GCAACTGGAG CACCTCCTAG TCAGACGGTT GCTCTGGTGA CACAACCTGT GGTTGCCAAG 
GAAACCGCTG TCTCCAAACT AGAAATGCCA TCTTCCTTGC TGTTGGAGGT CCCTGCACTG 
GCAGATTTCA ACCGGGCTTG GACAGAACTT ACCGACTGGC TGTCTCTGCT TGATCGAGTT 
CTGAAAGCAC AGAGGGTGAT GGTGGGTGAT CTTGAAGACA TCAACGAGAT GATCATCAAG 
CAGAAGGCAA CACTGCAGGA CTTGGAGCAG AGGCGCCCCC AGTTGGAAGA ACTCATTACT 
GCTGCCCAGA ATTTGAAAAA CAAGACCAGC AATCAAGAGG CCAGGACCAT CATTACTGAT 
CGAATTGAAA GAATTCAGAG TCAGTGGGAT GAAGTACAGG AACACCTTCA GAACCGGAGG 
CAGCAGTTAA ATGAAATGTT AAAGGATTCA ACACAATGGC TGGAAGCTAA GGAAGAAGCT 
GAGCAGGTGG TGGGGCAGGC CAGAGCCAAG CTTGAGACAT GGAAGGAGGG TCCCTACACA 
ATGGATGCAA TCCAAAGGAA GATCACAGAA ACCAAGCAGT TGGCCAAAGA CCTCCGCCAG 
TGGCAGATAA ATGTAGATGT GGCAAATGAC TTGGCACTGA AACTTCTCCG GGATTATTCT 
GCAGATGATA CCCGAAAGGT ACACATGATA ACAGAGAACA TCAATGCCTC TTGGGCAAGC 
ATTCATAAAA GAGTGAGTGA GCGAGAGACT GCTCTGGAAG AAACTCATAG ATTACTGCAA 
CAGTTCCCCC TGGACTTGGA GAAGTTTCTT GCCTGGCTTA CAGAAGCTGA AACAACTGCC 
AACGTCCTGC AGGATGCCAC CCATAAGGAA AGGCTTCTAG AAGACTCCAA GGGAGTAAGA 
GAGCTGATGA AACAATGGCA GGACCTCCAA GGAGAAATTG AAGCTCACAC AGATATCTAT 
CACAACCTAG ATGAAAATGG CCAAAAAATC CTGAGATCCC TGGAAGGTTC TGATGACGCG 
GTCCTATTAC AAAGACGTTT GGATAATATG AATTTCAAGT GGAGTGAGCT GCGGAAAAAG 
TCTCTCAATA TCAGGTCCCA TTTGGAAGCC AGTTCTGACC AGTGGAAGCG TCTGCACCTT 
TCTCTTCAGG AACTTCTGGT GTGGCTACAG CTGAAAGACG ATGAGTTGAG CCGTCAGGCA 
CCTATTGGAG GCGATTTCCC AGCAGTTCAG AAGCAGAATG ATATACACAG GGCCTTCAAG 
AGGGAACTGA AAACGAAAGA ACCTGTAATC ATGAGTACTC TTGAGACTGT ACGAATATTT 
CTGACAGAGC AGCCTTTGGA AGGACTAGAG AAACTCTACC AGGAGCCCAG AGAGCTGCCG 
CCTGAGGAGA AAGCCCAGAA TGTCACACGA CTCCTACGAA AGCAGGCTGA GGAGGTCAAC 
ACAGAGTGGG AAAAATTGAA CCTACACTCG GCTGACTGGC AGAGAAAAAT AGACGAGGCC 
CTCGAAAGAC TCCAAGAACT GCAGGAGGCC ACGGACGAGC TGGACCTCAA ACTGCGCCAA 
GCTGAGGTGA TCAAGGGATC ATGGCAGCCC GTGGGCGATC TTCTCATTGA CTCCCTCCAG 
GATCACCTCG AGAAAGTCAA GGCACTTCGA GGAGAAATTG CACCTCTCAA AGAGAATGTA 
AGCCACGTCA GTGACCTTGC TCGCCAACTC ACCACGTTGG GCATTCAGCT TTCACCATAT 
AATCTCAGCA CTCTGGAAGA CCTGAACACC AGATGGAAGC TTCTGCAGGT GGCTGTTGAG 
GACCGCATCA GGCAGCTGCA TGAAGCCCAC AGGGACTTTG GCCCAGCGTC CCAGCACTTC 
CTTTCCACTT CTGTCCAGGG TCCCTGGGAG AGAGCCATCT CACCAAACAA AGTGCCCTAC 
TATATCAACC ACGAGACCCA AACAACTTGC TGGGACCATC CTAAAATGAC AGAGCTCTAC 
CAGTCTTTAG CTGACCTGAA TAATGTCAGG TTCTCGGCTT ATAGGACTGC CATGAAACTC 
CGAAGACTGC AGAAGGCCCT TTGCTTGGAT CTTTTGAGCC TGTCAGCCGC ATGTGATGCC 
TTGGACCAGC ACAACCTCAA GCAAAACGAC CAGCCCATGG ACATCCTGCA GATCATTAAC 
TGTTTGACCA CTATTTATGA CCGCCTGGAG CAAGAGCACA ACAATCTGGT CAACGTCCCT 
CTCTGCGTGG ATATGTGTCT CAATTGGCTG CTGAATGTTT ATGATACGGG ACGAACAGGG 
AGGATCCGGG TCCTGTCTTT TAAAACTGGC ATCATTTCTC TGTGTAAAGC GCATTTGGAA 
GACAAGTACA GATACCTTTT CAAGCAAGTG GCCAGTTCAA CAGGATTTTG TGACCAGCGC 
AGACTGGGCC TCCTTCTACA TGACTCTATC CAAATCCCAA GACAGTTGGG TGAAGTTGCA 
TCCTTTGGGG GCAGTAACAT TGAGCCAAGT GTCAGGAGCT GCTTCCAATT CGCTAATAAT 
AAGCCTGAGA TCGAAGCAGC TCTCTTCCTC GATTGGATGA GACTAGAACC CCAGTCCATG 
GTGTGGCTGC CCGTCCTGCA CAGGGTGGCT GCCGCGGAGA CCGCCAAGCA TCAGGCCAAG 
TGTAACATCT GCAAGGAGTG TCCAATCATC GGCTTCAGGT ACAGAAGTCT AAAGCACTTT 
AATTATGACA TCTGCCAAAG CTGCTTTTTC TCTGGCCGCG TTGCAAAAGG CCATAAAATG 
CACTATCCCA TGGTGGAATA TTGTACTCCG ACTACATCGG GAGAAGATGT TCGAGACTTT 
GCCAAGGTAC TAAAAAACAA ATTTCGCACC AAAAGGTATT TTGCGAAGCA TCCCCGAATG 
GGCTACCTGC CAGTGCAGAC GGTCTTAGAG GGGGACAACA TGGAAACTCC TGTTACTCTG 
ATCAACTTCT GGCCGGTAGA CTCTGCGCCT GCCTCGTCCC CTCAGCTTTC ACACGATGAT 
ACTCATTCAC GCATTGAACA TTATGCTAGC AGGCTAGCAG AAATGGAAAA CAGCAGTGGA 
TCTTATCTAA ATGATAGCAT CTCTCCTAAT GAGAGCATAG ATGATGAACA TTTGTTAATC 
CAGCATTACT GCCAAAGTTT GAACCAGGAC TCCCCCCTGA GCCAGCCTCG TAGTCCTGCC 
CAGATCTTGA TTTCCTTAGA GAGTGAGGAA AGAGGGGAGC TAGAGAGAAT CCTAGCAGAT 
CTTGAGGAAG AAAACAGGAA TCTGCAGGCT GAATATGATC GTCTCAAGGA GCAGCATGAA 
CATAAAGGCC TGTCCCCACT GCCGTCCCCT CCTGAGATGA TGCCCACCTC GCCACAGAGT 
CCCCGGGATG CTGAGCTCAT TGCTGAGGCC AAGCTACTGC GTCAACACAA AGGCCGCCTG 
GAAGCCAGGA TGCAAATCCT GGAAGACCAC AATAAACAGC TGGAGTCCCA GTTACACAGG 
CTCAGGCAGC TGCTGGAGCA ACCCCAGGCT GAGGCCAAGG TGAATGGCAC AACGGTGTCC 
TCTCCTTCTA CCTCTCTTCA GAGGTCAGAC AGCAGCCAGC CTATGCTGCT CCGGGTGGTT 
GGCAGTCAAA CTTCAGAATC CATGGGCGAG GAAGACCTTC TCAGTCCTCC CCAGGACTCA 
AGCACAGGGT TAGAGGAAGT GATGGAGCAA CTCAACAACT CCTTCCCTAG TTCCAGAGGA 
CACAATGTAG GAAGCCTTTT CCACTTGGCA GATGATTTGG GCAGAGCGAT GGAGTCCTTA 
GTTTCAGTTA TGACAGATGA AGAAGGGGCA GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_024988366.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844134.1 Bos taurus dystrophin (DMD), transcript variant X8, mRNA.

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CloneID	OBa238544
Clone ID Related Accession (Same CDS sequence)	XM_024988366.1
Accession Version	XM_024988366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAATTTTCT AAGTTCGGGA AGCAGCACAT AGAAAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTC TTGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCAGGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAATA TCGGAAGCTC TGACATAGTA 
GATGGAAATC ATAAACTGAC TCTTGGCTTG ATTTGGAATA TAATCCTCCA TTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA GAAGATACTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT CAACTTCACC 
ACCAGCTGGT CTGACGGTTT GGCTTTGAAT GCGCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TCGCCAGCAG TCAGCTACAC AACGACTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTACTGG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TACGTCACAT CTCTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCCTTGAAGC CATCCAGGAG GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACAAGAGAAG AACATTTTCA ACTACATCAT CAAATGCACT ACTCTCAACA GATCACTGTC 
AGTCTAGCAC AGGGCTATGA ACGAACTCCT TCCTCTCCTC AGCCTCGCTT CAAGAGCTAT 
GCCTACACGC AGGCTGCTTA TGTCTCCACC TCTGACCCCA CACGGAGCCC ATTTCCTTCA 
CAGCGTTTGG AAGCTCCTGA AGACAAGTCA TTTGGCAGTC CATTGATGGA GACTGAAGTA 
AACCTGGACA GTTACCAGAC AGCTTTAGAA GAAGTACTCT CATGGCTCCT TTCAGCCGAA 
GACACATTGC AAGCACAAGG AGAGATTTCT AATGATGTAG AAGAAGTGAA AGAACAGTTT 
CATACCCACG AGGGATACAT GATGGATTTG ACATCCCATC AGGGACGGGT TGGCAATGTT 
CTCCAATTGG GGAGTCAGCT GATTGGGTCA GGGAAATTAT CAGAAGATGA AGAAACTGAA 
GTGCAAGAAC AAATGAATCT TCTAAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTC ATGGATCTCC AGAATCAGCA GCTGAAAGAG 
TTAAATGCCT GGTTAACAAA AACAGAAGAG AGAACAAGGA AAATGGAAAA AGAGCCCCTT 
GGACCTGATC TTGAGGACCT CAAACGCCAA ATACAGCAAC ATAAGGTGCT TCAAGAAGAC 
CTAGAACAGG AACAAGTCAG AGTCAATTCT CTCACGCACA TGGTGGTGGT TGTAGATGAG 
TCTAGTGGTG ATCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGG 
TGGGCAAACA TCTGTAGGTG GACTGAAGAT CGCTGGGTTC TTTTACAGGA TGTTCTTCTA 
AAATGGCAAC GTTTTACTGA AGAGCAGTGC CTTTTTAGTA CATGGCTTTC AGATAAAGAA 
GATGCATTGA ACAAGATTCC CACAAGTGGC TTTAAGGATC AAAGTGAAAT GTTATCAAGT 
CTTCAAAAAC TGGCCGTTTT AAAAACTGAT CTAGAAAAGA AAAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCATGA TCTCCTTTCA ACACTGAAAA ATACATTGGT TGCCCAAAAG 
ATGGAAGCAT GGTTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA AAAACTTGAA 
AAGAGTTCAA CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACGGTAAC TATGGTGACC ACGAGGGAGC AAATCCTGGT AAAGCATGCC 
CAAGAGGAAC TCCCTCCACC ACCTCCACAA AAGAAGAGGC AGATTATTGT GGATTCTGAA 
ATTAAGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCCGTGTTAC AGAGTCCTGA ATTTGCAGTC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGTATCAAAC AAGCCTCAGA ACAACTGAAC AGCCGGTGGA TCGAGTTCTG CCAATTGCTA 
AGTGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCA GAAAACTGGT TGAAAACTCA ACCCACTACC 
CCATCAGAGC CAACAGCAGT TAAAAGCCAG TTAAAAAATT GTAAGGATGA AGTCAACCGG 
CTGTCAGCTC TTCAGCCTCA AATCGAGAGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCC GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATATGCA GGCCAGAGAG AAAGAGTTAC AGACAATTTT GGACACTTTA 
CCCACCGTGC GGTATCAGGA GACCATGAGT ACCATCCTCA CGTGGATCCA ACAGTCAGAA 
ACCAAACTCT CGATACCTCA GGTTACTGTC ACTGAATACG AGATCATGGA GCAGAGACTT 
GAGGAGTTAC AGGCTTTACA AAGCTCTCTG CAAGAGCACC AAAATGACCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAGGAAA GCACCCTCTC ATATAAGCCA GAGATATCAG 
TCAGAATTTG AAAACATTGA GGGTCGGTGG AAGAAACTCT CTGCCCAGCT GAATGAACGT 
TGCCAAAAGC TAGAGGAGCA AATGGCCAAA CTCCGAAAAC TTCAGAATCA CATAAAAACA 
CTGAAGAAGT GGATGGCTGA AGTTGATGTT TTCTTGAAAG ATGAATGGCC TGCCCTTGGA 
GATTCAGAAA TTCTTAAAAA ACAGCTGAAG CAGTGCAGGC TTTTAGTCAG TGATATTCAG 
ACAATCCAGC CCAGTCTAAA CAGTGTCAAT GAAGGTGGAC AGAAAATAAA AACTGAAGCG 
GAGCCAGAGT TTGCTTCTAG ACTGGAGACA GAGCTCAGAG AACTTAACAC TCAATGGGAT 
TACATATGTC GACAGGTCTA TGCCAGAAAG GAGGCCTTAA AGGGAGGCTT GGATAAAACT 
GTAAGCCTTC AGAAAGATTT GTCAGAGATG CATGAATGGA TGACACAAGC CGAAGAAGAA 
TACCTAGAGA GAGATTTTGA ATATAAAACC CCAGATGAAT TACAGACAGC AGTTGAAGAG 
ATGAAGAGAG CTAAAGAAGA GGCCCAGCAA AAAGAAGCAA AAGTGCAGCT CCTCACTGAG 
TCCGTGAACA GTGTCATAGC TCAAGCTCCA CCTGCAGCAC AAGAGGCCTT AAGAAAGGAA 
CTGGACACTC TGACCACCAA CTACCAGTGG CTCTGCACCA GGCTGAATGG AAAATGCAAG 
ACGTTAGAAG AGGTTTGGGC ATGTTGGCAT GAGTTATTGT CATACTTGGA AAAGGCAAAT 
AAGTGGCTAA ATGAAGTAGA ATTGAAACTT AAGACTACTG AAAATATTCC TGGTGGAGCT 
GAGGAAATCT CTGAGGTGCT CAGTTCACTT GAAAATTTGA TGCAACATTC AGAAGATAAC 
CCGAATCAGA TTCGCATATT GGCACAGACC CTAACAGATG GTGGAGTCAT GGATGAACTG 
ATCAATGAAG AACTCGAGAC ATTTAATTCT CGTTGGAGAG AACTACATGA AGAGGCTGTG 
AGGAGGCAAA AGTTGCTTGA ACAGAGTATC CAGTCTGCCC AGGAGATTGA AAAATCCTTA 
CAGTTAATTC AGGAATCCCT CTCATCAATT GACAAGCAGT TGGCAGCTTA TATCGCTGAC 
AAAGTGGACG CCGCTCAGAT GCCTCAGGAA GCCCAGAAAA TCCAATCTGA TTTGACAAGT 
CATGAGATCA GTTTAGAAGA AATGAAGAAA CATTACCAAG GAAAGGAAAC TGCTCAACGG 
GTACTATCCC AAATTGAAGT GGCACAGAAA AAAATGCAAG ATGTTTCCAT GAAATTTCGA 
TTGTTCCAGA AGCCAGCCAA TTTTGAGCAG CGTCTACAGG AAAGTAAGAT GATTTTAGAT 
GAAGTGAAGA TGCACTTGCC CGCACTGGAA ACAAAGAGTG TTGAACAGGA AGTAGTACAG 
TCACAGTTAA ATCATTGTGT GAACTTGTAT AAAAGTCTGA GTGAAGTGAA GTCTGAAGTG 
GAAATGGTGA TTAAAACTGG ACGTCAAATT GTACAGAAGA AGCAGACGGA AAACCCTAAA 
GAGCTTGATG AAAGAGTAAC GGCTTTGAAA TTGCATTATA ATGAGCTGGG AGCAAAGGTA 
ACAGAAAGAA AGCAACAGTT GGAGAAATGC TTGAAATTGT CCCGTAAGAT GCGAAAGGAA 
ATGAATGCCT TGACAGAATG GCTGGCAGCT ACAGATTTGG AACTGACAAA GAGATCAGCA 
GTTGAAGGAA TGCCTAGTAA TTTGGATTCT GAAGTTGCCT GGGGAAAGGC TACTCAGAAA 
GAGACTGAGA AACAGAAGGT TCACCTAAAG AGCATTACAG AGCTAGGAGA GGCCTTGAAA 
ACGGTTTTGG GCAAGAAGGA GACCTTGGTC GAAGATAAAC TGAGTCTTTT GAATAGTAAC 
TGGATAGCCG TCACTTCTCG AGCAGAAGAG TGGCTAAACC TTTTGTTGGA ATACCAGAAA 
CACATGGAAA CCTTTGACCA GAATGTGGAT CATATCACAA AGTGGATCAT TCAAGCTGAT 
GCACTTCTGG ATGAATCTGA GAAAAAGAAA CCACAGCAAA AAGAAGACAT GCTAAAGCGC 
TTAAAGGCTG AACTGAATGA CATACGCCCA AAGGTGGACT CCACGCGTGA CCAAGCAGCA 
AACTTGATGG CAAACCGCGG CGACCACTGC AGGAAAGTAA TAGAGCCCAA AATCTCAGAG 
CTCAACCATC GATTTGCAGC CATTTCTCAC AGAATTAAGA CTGGAAAGGC CTCCATTCCT 
TTGAAGGAAT TGGAGCAGTT TAACTCAGAT ATACAAAAAT TGCTTGAACC ACTGGAGGCT 
GAAATTCAGC AGGGGGTGAA TCTGAAAGAG GAAGACTTCA ATAAAGATAT GAGTGAAGAC 
AATGAGGGTA CTGTAAAAGA ATTGTTGCAA AGAGGAGACA ACTTACAACA AAGAATCACA 
GATGAGAGAA AGCGAGAGGA AATAAAGATA AAACAGCAGC TGTTACAGAC AAAACATAAT 
GCTCTCAAGG ATTTGAGGTC TCAAAGAAGA AAAAAGGCTC TAGAAATTTC TCACCAGTGG 
TATCAGTACA AGAGGCAGGC TGATGATCTC CTGAAATGCT TGGATGACAT TGAAAAAAAA 
TTAGCCAGCC TACCTGAACC CAGAGATGAA AGGAAAATAA AGGAAATTGA TCGTGAGTTG 
CAGAAGAAGA AAGAGGAGCT GAATGCAGTG CATAGGCAAG CTGAGGGCTT GTCTGAGGAT 
GGGGCAGCAA TGGCAGTGGA GTCAACTCAG ATCCAGCTCA GCAAGCGCTG GCGGGAAATT 
GAGAGCAAAT TTGCTCAGTT TCGAAGACTC AACTTTGCAC AAATTCACAC TGTCCATGAA 
GAATCAGTGA TGGTGATGAC CGAAGACATG CCTTTGGAAA TTTCTTATGT GCCTTCTACT 
TACCTGACTG AGATCACTCA CGTCTTACAA GCCCTTTCAG AAGTGGAACA ACTTCTCAAT 
GCTCCCGACC TCTGTGCTAA AGATTTTCAA GATCTCTTCA AACAAGAGGA ATCTCTGAAG 
AATATAAAAG ACAACCTGCA ACAAATCTCA GGTCGGATTG ATGTTATTCA CAATAAGAAG 
GCGGCAGCAT TGCAAAGCAC CACACCCCCA GAAAAGGCAA GGTTACAGGA AGCTCTCTCC 
AGGCTCGACT TCCAATGGGA AAGAGTGAAC AAAATGTACA AGGACCGACA AGGGCAATTT 
GACAGATCTG TTGAAAAATG GCGACGGTTT CATTATGATA TGAAGATATT TAATCAATGG 
CTAACAGAAG CTGAACACTT TCTCAAAAAG ACACAAATTC CTGAGAACTG GGAACATGCC 
AAATATAAAT GGTATCTTAA GGAACTCCAG GATGGCATCG GGCAGCGGCA AACTGTTGTC 
AGAGTATTGA ATGCAACTGG GGAAGAAATA ATTCAGCAGT CCTCAAAAAT AGATGCCAGT 
ATTCTACAAG AAAAATTGGG AAGCCTGAAT CTGCGGTGGC AGGAGGTCTG CAAACAGCTG 
GCCGAGAGAA AAAAGAGGTT AGAAGAACAA AAGAATATCC TGTCAGAATT TCAAAGAGAT 
TTGAATGAAT TTGTTTTATG GTTGGAAGAA GCAGGTAACA TTTCTAGTAT TCCTCTTGAA 
CCTGGAAATG AGCAGCAACT AAAAGAAAAG CTTGAGGAAG TCAAGTTACT GGCCGAAGAG 
TTGCCCCTGC GTCAGGGAAT TCTAAAACAA TTAAATGAAA CAGGAGGAAC AGTGCTTGTA 
AGTGCTCCCA TAAGCCCAGA AGAGCAAGAT AAACTTGAAA ATAAGCTCAA GCAGACAAAT 
CTTCAGTGGA TAAAGGTTTC TAGGAGTTTA CCTGAGAAAC AGGGAGAAAT TGAGGCTCAC 
ATAAAAGACC TTGGACAGCT TGAAGAGCAA TTAAATCATC TGCTTCTGTG GCTGTCTCCT 
ATTAGGAGTC AGTTGGAAAT TTACAACCAA CCAAATCAGA CAGGACCATT TGACATTAAG 
GAAACTGAAG TAGCAGTTCA AGCTAAACAG CCGGATGTGG AAGGGATTTT GTCTAAAGGG 
CAGAATTTGT ACAAGGAAAA ACCAGCCACT GAGCCAGTGA AGAGGAAGTT AGAAGATCTG 
AGCTCGGAGT GGAAGGCGGT AACCCACTTA CTTCAAGAGC TGAGGGCAAA GTGGCCTGGC 
CCAGTCCCTG GACTGACAGC AACTGGAGCA CCTCCTAGTC AGACGGTTGC TCTGGTGACA 
CAACCTGTGG TTGCCAAGGA AACCGCTGTC TCCAAACTAG AAATGCCATC TTCCTTGCTG 
TTGGAGGTCC CTGCACTGGC AGATTTCAAC CGGGCTTGGA CAGAACTTAC CGACTGGCTG 
TCTCTGCTTG ATCGAGTTCT GAAAGCACAG AGGGTGATGG TGGGTGATCT TGAAGACATC 
AACGAGATGA TCATCAAGCA GAAGGCAACA CTGCAGGACT TGGAGCAGAG GCGCCCCCAG 
TTGGAAGAAC TCATTACTGC TGCCCAGAAT TTGAAAAACA AGACCAGCAA TCAAGAGGCC 
AGGACCATCA TTACTGATCG AATTGAAAGA ATTCAGAGTC AGTGGGATGA AGTACAGGAA 
CACCTTCAGA ACCGGAGGCA GCAGTTAAAT GAAATGTTAA AGGATTCAAC ACAATGGCTG 
GAAGCTAAGG AAGAAGCTGA GCAGGTGGTG GGGCAGGCCA GAGCCAAGCT TGAGACATGG 
AAGGAGGGTC CCTACACAAT GGATGCAATC CAAAGGAAGA TCACAGAAAC CAAGCAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTGG CAAATGACTT GGCACTGAAA 
CTTCTCCGGG ATTATTCTGC AGATGATACC CGAAAGGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT TCATAAAAGA GTGAGTGAGC GAGAGACTGC TCTGGAAGAA 
ACTCATAGAT TACTGCAACA GTTCCCCCTG GACTTGGAGA AGTTTCTTGC CTGGCTTACA 
GAAGCTGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GACTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAGG ACCTCCAAGG AGAAATTGAA 
GCTCACACAG ATATCTATCA CAACCTAGAT GAAAATGGCC AAAAAATCCT GAGATCCCTG 
GAAGGTTCTG ATGACGCGGT CCTATTACAA AGACGTTTGG ATAATATGAA TTTCAAGTGG 
AGTGAGCTGC GGAAAAAGTC TCTCAATATC AGGTCCCATT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTGT GGCTACAGCT GAAAGACGAT 
GAGTTGAGCC GTCAGGCACC TATTGGAGGC GATTTCCCAG CAGTTCAGAA GCAGAATGAT 
ATACACAGGG CCTTCAAGAG GGAACTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTGGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCGCC TGAGGAGAAA GCCCAGAATG TCACACGACT CCTACGAAAG 
CAGGCTGAGG AGGTCAACAC AGAGTGGGAA AAATTGAACC TACACTCGGC TGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAAGAACTGC AGGAGGCCAC GGACGAGCTG 
GACCTCAAAC TGCGCCAAGC TGAGGTGATC AAGGGATCAT GGCAGCCCGT GGGCGATCTT 
CTCATTGACT CCCTCCAGGA TCACCTCGAG AAAGTCAAGG CACTTCGAGG AGAAATTGCA 
CCTCTCAAAG AGAATGTAAG CCACGTCAGT GACCTTGCTC GCCAACTCAC CACGTTGGGC 
ATTCAGCTTT CACCATATAA TCTCAGCACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CTGTTGAGGA CCGCATCAGG CAGCTGCATG AAGCCCACAG GGACTTTGGC 
CCAGCGTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCT 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCGGCTTAT 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT TTTGAGCCTG 
TCAGCCGCAT GTGATGCCTT GGACCAGCAC AACCTCAAGC AAAACGACCA GCCCATGGAC 
ATCCTGCAGA TCATTAACTG TTTGACCACT ATTTATGACC GCCTGGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GATACGGGAC GAACAGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCGC ATTTGGAAGA CAAGTACAGA TACCTTTTCA AGCAAGTGGC CAGTTCAACA 
GGATTTTGTG ACCAGCGCAG ACTGGGCCTC CTTCTACATG ACTCTATCCA AATCCCAAGA 
CAGTTGGGTG AAGTTGCATC CTTTGGGGGC AGTAACATTG AGCCAAGTGT CAGGAGCTGC 
TTCCAATTCG CTAATAATAA GCCTGAGATC GAAGCAGCTC TCTTCCTCGA TTGGATGAGA 
CTAGAACCCC AGTCCATGGT GTGGCTGCCC GTCCTGCACA GGGTGGCTGC CGCGGAGACC 
GCCAAGCATC AGGCCAAGTG TAACATCTGC AAGGAGTGTC CAATCATCGG CTTCAGGTAC 
AGAAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGCT GCTTTTTCTC TGGCCGCGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATATT GTACTCCGAC TACATCGGGA 
GAAGATGTTC GAGACTTTGC CAAGGTACTA AAAAACAAAT TTCGCACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACGG TCTTAGAGGG GGACAACATG 
GAAACGCTAG CAGAAATGGA AAACAGCAGT GGATCTTATC TAAATGATAG CATCTCTCCT 
AATGAGAGCA TAGATGATGA ACATTTGTTA ATCCAGCATT ACTGCCAAAG TTTGAACCAG 
GACTCCCCCC TGAGCCAGCC TCGTAGTCCT GCCCAGATCT TGATTTCCTT AGAGAGTGAG 
GAAAGAGGGG AGCTAGAGAG AATCCTAGCA GATCTTGAGG AAGAAAACAG GAATCTGCAG 
GCTGAATATG ATCGTCTCAA GGAGCAGCAT GAACATAAAG GCCTGTCCCC ACTGCCGTCC 
CCTCCTGAGA TGATGCCCAC CTCGCCACAG AGTCCCCGGG ATGCTGAGCT CATTGCTGAG 
GCCAAGCTAC TGCGTCAACA CAAAGGCCGC CTGGAAGCCA GGATGCAAAT CCTGGAAGAC 
CACAATAAAC AGCTGGAGTC CCAGTTACAC AGGCTCAGGC AGCTGCTGGA GCAACCCCAG 
GCTGAGGCCA AGGTGAATGG CACAACGGTG TCCTCTCCTT CTACCTCTCT TCAGAGGTCA 
GACAGCAGCC AGCCTATGCT GCTCCGGGTG GTTGGCAGTC AAACTTCAGA ATCCATGGGC 
GAGGAAGACC TTCTCAGTCC TCCCCAGGAC TCAAGCACAG GGTTAGAGGA AGTGATGGAG 
CAACTCAACA ACTCCTTCCC TAGTTCCAGA GGAAGAAATA CCCCTGGGAA GCCAATGAGA 
GAGGACACAA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844134.1
CDS	252..11186
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALQVLQKNNVDLVNIGSSDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVRQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYVTSLFQVLPQQVSLEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERTPSSPQPRFKSYAYTQAAYVSTSDPTRSPFPSQRLEAPEDKSFGSPLMETEVNLDSYQTALEEVLSWLLSAEDTLQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGSGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNAWLTKTEERTRKMEKEPLGPDLEDLKRQIQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDVLLKWQRFTEEQCLFSTWLSDKEDALNKIPTSGFKDQSEMLSSLQKLAVLKTDLEKKKQTMDKLCSLNHDLLSTLKNTLVAQKMEAWLDNFAQRWDNLVQKLEKSSTQISQAVTTTQPSLTQTTVMETVTMVTTREQILVKHAQEELPPPPPQKKRQIIVDSEIKKRLDVDITELHSWITRSEAVLQSPEFAVYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTPSEPTAVKSQLKNCKDEVNRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADMQAREKELQTILDTLPTVRYQETMSTILTWIQQSETKLSIPQVTVTEYEIMEQRLEELQALQSSLQEHQNDLNYLSTTVKEMSRKAPSHISQRYQSEFENIEGRWKKLSAQLNERCQKLEEQMAKLRKLQNHIKTLKKWMAEVDVFLKDEWPALGDSEILKKQLKQCRLLVSDIQTIQPSLNSVNEGGQKIKTEAEPEFASRLETELRELNTQWDYICRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVQLLTESVNSVIAQAPPAAQEALRKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLNEVELKLKTTENIPGGAEEISEVLSSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLQLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHYQGKETAQRVLSQIEVAQKKMQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDLELTKRSAVEGMPSNLDSEVAWGKATQKETEKQKVHLKSITELGEALKTVLGKKETLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMETFDQNVDHITKWIIQADALLDESEKKKPQQKEDMLKRLKAELNDIRPKVDSTRDQAANLMANRGDHCRKVIEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVHRQAEGLSEDGAAMAVESTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVMVMTEDMPLEISYVPSTYLTEITHVLQALSEVEQLLNAPDLCAKDFQDLFKQEESLKNIKDNLQQISGRIDVIHNKKAAALQSTTPPEKARLQEALSRLDFQWERVNKMYKDRQGQFDRSVEKWRRFHYDMKIFNQWLTEAEHFLKKTQIPENWEHAKYKWYLKELQDGIGQRQTVVRVLNATGEEIIQQSSKIDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDLNEFVLWLEEAGNISSIPLEPGNEQQLKEKLEEVKLLAEELPLRQGILKQLNETGGTVLVSAPISPEEQDKLENKLKQTNLQWIKVSRSLPEKQGEIEAHIKDLGQLEEQLNHLLLWLSPIRSQLEIYNQPNQTGPFDIKETEVAVQAKQPDVEGILSKGQNLYKEKPATEPVKRKLEDLSSEWKAVTHLLQELRAKWPGPVPGLTATGAPPSQTVALVTQPVVAKETAVSKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVLKAQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVVGQARAKLETWKEGPYTMDAIQRKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRVSERETALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKILRSLEGSDDAVLLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDIHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEEKAQNVTRLLRKQAEEVNTEWEKLNLHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVSHVSDLARQLTTLGIQLSPYNLSTLEDLNTRWKLLQVAVEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETLAEMENSSGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKEQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDSSTGLEEVMEQLNNSFPSSRGRNTPGKPMREDTM

Target ORF information:

RefSeq Version	XM_024988366.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophin (DMD), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988366.1

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAATTTTCT AAGTTCGGGA AGCAGCACAT AGAAAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTC TTGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCAGGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAATA TCGGAAGCTC TGACATAGTA 
GATGGAAATC ATAAACTGAC TCTTGGCTTG ATTTGGAATA TAATCCTCCA TTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA GAAGATACTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT CAACTTCACC 
ACCAGCTGGT CTGACGGTTT GGCTTTGAAT GCGCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TCGCCAGCAG TCAGCTACAC AACGACTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTACTGG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TACGTCACAT CTCTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCCTTGAAGC CATCCAGGAG GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACAAGAGAAG AACATTTTCA ACTACATCAT CAAATGCACT ACTCTCAACA GATCACTGTC 
AGTCTAGCAC AGGGCTATGA ACGAACTCCT TCCTCTCCTC AGCCTCGCTT CAAGAGCTAT 
GCCTACACGC AGGCTGCTTA TGTCTCCACC TCTGACCCCA CACGGAGCCC ATTTCCTTCA 
CAGCGTTTGG AAGCTCCTGA AGACAAGTCA TTTGGCAGTC CATTGATGGA GACTGAAGTA 
AACCTGGACA GTTACCAGAC AGCTTTAGAA GAAGTACTCT CATGGCTCCT TTCAGCCGAA 
GACACATTGC AAGCACAAGG AGAGATTTCT AATGATGTAG AAGAAGTGAA AGAACAGTTT 
CATACCCACG AGGGATACAT GATGGATTTG ACATCCCATC AGGGACGGGT TGGCAATGTT 
CTCCAATTGG GGAGTCAGCT GATTGGGTCA GGGAAATTAT CAGAAGATGA AGAAACTGAA 
GTGCAAGAAC AAATGAATCT TCTAAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTC ATGGATCTCC AGAATCAGCA GCTGAAAGAG 
TTAAATGCCT GGTTAACAAA AACAGAAGAG AGAACAAGGA AAATGGAAAA AGAGCCCCTT 
GGACCTGATC TTGAGGACCT CAAACGCCAA ATACAGCAAC ATAAGGTGCT TCAAGAAGAC 
CTAGAACAGG AACAAGTCAG AGTCAATTCT CTCACGCACA TGGTGGTGGT TGTAGATGAG 
TCTAGTGGTG ATCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGG 
TGGGCAAACA TCTGTAGGTG GACTGAAGAT CGCTGGGTTC TTTTACAGGA TGTTCTTCTA 
AAATGGCAAC GTTTTACTGA AGAGCAGTGC CTTTTTAGTA CATGGCTTTC AGATAAAGAA 
GATGCATTGA ACAAGATTCC CACAAGTGGC TTTAAGGATC AAAGTGAAAT GTTATCAAGT 
CTTCAAAAAC TGGCCGTTTT AAAAACTGAT CTAGAAAAGA AAAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCATGA TCTCCTTTCA ACACTGAAAA ATACATTGGT TGCCCAAAAG 
ATGGAAGCAT GGTTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA AAAACTTGAA 
AAGAGTTCAA CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACGGTAAC TATGGTGACC ACGAGGGAGC AAATCCTGGT AAAGCATGCC 
CAAGAGGAAC TCCCTCCACC ACCTCCACAA AAGAAGAGGC AGATTATTGT GGATTCTGAA 
ATTAAGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCCGTGTTAC AGAGTCCTGA ATTTGCAGTC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGTATCAAAC AAGCCTCAGA ACAACTGAAC AGCCGGTGGA TCGAGTTCTG CCAATTGCTA 
AGTGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCA GAAAACTGGT TGAAAACTCA ACCCACTACC 
CCATCAGAGC CAACAGCAGT TAAAAGCCAG TTAAAAAATT GTAAGGATGA AGTCAACCGG 
CTGTCAGCTC TTCAGCCTCA AATCGAGAGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCC GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATATGCA GGCCAGAGAG AAAGAGTTAC AGACAATTTT GGACACTTTA 
CCCACCGTGC GGTATCAGGA GACCATGAGT ACCATCCTCA CGTGGATCCA ACAGTCAGAA 
ACCAAACTCT CGATACCTCA GGTTACTGTC ACTGAATACG AGATCATGGA GCAGAGACTT 
GAGGAGTTAC AGGCTTTACA AAGCTCTCTG CAAGAGCACC AAAATGACCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAGGAAA GCACCCTCTC ATATAAGCCA GAGATATCAG 
TCAGAATTTG AAAACATTGA GGGTCGGTGG AAGAAACTCT CTGCCCAGCT GAATGAACGT 
TGCCAAAAGC TAGAGGAGCA AATGGCCAAA CTCCGAAAAC TTCAGAATCA CATAAAAACA 
CTGAAGAAGT GGATGGCTGA AGTTGATGTT TTCTTGAAAG ATGAATGGCC TGCCCTTGGA 
GATTCAGAAA TTCTTAAAAA ACAGCTGAAG CAGTGCAGGC TTTTAGTCAG TGATATTCAG 
ACAATCCAGC CCAGTCTAAA CAGTGTCAAT GAAGGTGGAC AGAAAATAAA AACTGAAGCG 
GAGCCAGAGT TTGCTTCTAG ACTGGAGACA GAGCTCAGAG AACTTAACAC TCAATGGGAT 
TACATATGTC GACAGGTCTA TGCCAGAAAG GAGGCCTTAA AGGGAGGCTT GGATAAAACT 
GTAAGCCTTC AGAAAGATTT GTCAGAGATG CATGAATGGA TGACACAAGC CGAAGAAGAA 
TACCTAGAGA GAGATTTTGA ATATAAAACC CCAGATGAAT TACAGACAGC AGTTGAAGAG 
ATGAAGAGAG CTAAAGAAGA GGCCCAGCAA AAAGAAGCAA AAGTGCAGCT CCTCACTGAG 
TCCGTGAACA GTGTCATAGC TCAAGCTCCA CCTGCAGCAC AAGAGGCCTT AAGAAAGGAA 
CTGGACACTC TGACCACCAA CTACCAGTGG CTCTGCACCA GGCTGAATGG AAAATGCAAG 
ACGTTAGAAG AGGTTTGGGC ATGTTGGCAT GAGTTATTGT CATACTTGGA AAAGGCAAAT 
AAGTGGCTAA ATGAAGTAGA ATTGAAACTT AAGACTACTG AAAATATTCC TGGTGGAGCT 
GAGGAAATCT CTGAGGTGCT CAGTTCACTT GAAAATTTGA TGCAACATTC AGAAGATAAC 
CCGAATCAGA TTCGCATATT GGCACAGACC CTAACAGATG GTGGAGTCAT GGATGAACTG 
ATCAATGAAG AACTCGAGAC ATTTAATTCT CGTTGGAGAG AACTACATGA AGAGGCTGTG 
AGGAGGCAAA AGTTGCTTGA ACAGAGTATC CAGTCTGCCC AGGAGATTGA AAAATCCTTA 
CAGTTAATTC AGGAATCCCT CTCATCAATT GACAAGCAGT TGGCAGCTTA TATCGCTGAC 
AAAGTGGACG CCGCTCAGAT GCCTCAGGAA GCCCAGAAAA TCCAATCTGA TTTGACAAGT 
CATGAGATCA GTTTAGAAGA AATGAAGAAA CATTACCAAG GAAAGGAAAC TGCTCAACGG 
GTACTATCCC AAATTGAAGT GGCACAGAAA AAAATGCAAG ATGTTTCCAT GAAATTTCGA 
TTGTTCCAGA AGCCAGCCAA TTTTGAGCAG CGTCTACAGG AAAGTAAGAT GATTTTAGAT 
GAAGTGAAGA TGCACTTGCC CGCACTGGAA ACAAAGAGTG TTGAACAGGA AGTAGTACAG 
TCACAGTTAA ATCATTGTGT GAACTTGTAT AAAAGTCTGA GTGAAGTGAA GTCTGAAGTG 
GAAATGGTGA TTAAAACTGG ACGTCAAATT GTACAGAAGA AGCAGACGGA AAACCCTAAA 
GAGCTTGATG AAAGAGTAAC GGCTTTGAAA TTGCATTATA ATGAGCTGGG AGCAAAGGTA 
ACAGAAAGAA AGCAACAGTT GGAGAAATGC TTGAAATTGT CCCGTAAGAT GCGAAAGGAA 
ATGAATGCCT TGACAGAATG GCTGGCAGCT ACAGATTTGG AACTGACAAA GAGATCAGCA 
GTTGAAGGAA TGCCTAGTAA TTTGGATTCT GAAGTTGCCT GGGGAAAGGC TACTCAGAAA 
GAGACTGAGA AACAGAAGGT TCACCTAAAG AGCATTACAG AGCTAGGAGA GGCCTTGAAA 
ACGGTTTTGG GCAAGAAGGA GACCTTGGTC GAAGATAAAC TGAGTCTTTT GAATAGTAAC 
TGGATAGCCG TCACTTCTCG AGCAGAAGAG TGGCTAAACC TTTTGTTGGA ATACCAGAAA 
CACATGGAAA CCTTTGACCA GAATGTGGAT CATATCACAA AGTGGATCAT TCAAGCTGAT 
GCACTTCTGG ATGAATCTGA GAAAAAGAAA CCACAGCAAA AAGAAGACAT GCTAAAGCGC 
TTAAAGGCTG AACTGAATGA CATACGCCCA AAGGTGGACT CCACGCGTGA CCAAGCAGCA 
AACTTGATGG CAAACCGCGG CGACCACTGC AGGAAAGTAA TAGAGCCCAA AATCTCAGAG 
CTCAACCATC GATTTGCAGC CATTTCTCAC AGAATTAAGA CTGGAAAGGC CTCCATTCCT 
TTGAAGGAAT TGGAGCAGTT TAACTCAGAT ATACAAAAAT TGCTTGAACC ACTGGAGGCT 
GAAATTCAGC AGGGGGTGAA TCTGAAAGAG GAAGACTTCA ATAAAGATAT GAGTGAAGAC 
AATGAGGGTA CTGTAAAAGA ATTGTTGCAA AGAGGAGACA ACTTACAACA AAGAATCACA 
GATGAGAGAA AGCGAGAGGA AATAAAGATA AAACAGCAGC TGTTACAGAC AAAACATAAT 
GCTCTCAAGG ATTTGAGGTC TCAAAGAAGA AAAAAGGCTC TAGAAATTTC TCACCAGTGG 
TATCAGTACA AGAGGCAGGC TGATGATCTC CTGAAATGCT TGGATGACAT TGAAAAAAAA 
TTAGCCAGCC TACCTGAACC CAGAGATGAA AGGAAAATAA AGGAAATTGA TCGTGAGTTG 
CAGAAGAAGA AAGAGGAGCT GAATGCAGTG CATAGGCAAG CTGAGGGCTT GTCTGAGGAT 
GGGGCAGCAA TGGCAGTGGA GTCAACTCAG ATCCAGCTCA GCAAGCGCTG GCGGGAAATT 
GAGAGCAAAT TTGCTCAGTT TCGAAGACTC AACTTTGCAC AAATTCACAC TGTCCATGAA 
GAATCAGTGA TGGTGATGAC CGAAGACATG CCTTTGGAAA TTTCTTATGT GCCTTCTACT 
TACCTGACTG AGATCACTCA CGTCTTACAA GCCCTTTCAG AAGTGGAACA ACTTCTCAAT 
GCTCCCGACC TCTGTGCTAA AGATTTTCAA GATCTCTTCA AACAAGAGGA ATCTCTGAAG 
AATATAAAAG ACAACCTGCA ACAAATCTCA GGTCGGATTG ATGTTATTCA CAATAAGAAG 
GCGGCAGCAT TGCAAAGCAC CACACCCCCA GAAAAGGCAA GGTTACAGGA AGCTCTCTCC 
AGGCTCGACT TCCAATGGGA AAGAGTGAAC AAAATGTACA AGGACCGACA AGGGCAATTT 
GACAGATCTG TTGAAAAATG GCGACGGTTT CATTATGATA TGAAGATATT TAATCAATGG 
CTAACAGAAG CTGAACACTT TCTCAAAAAG ACACAAATTC CTGAGAACTG GGAACATGCC 
AAATATAAAT GGTATCTTAA GGAACTCCAG GATGGCATCG GGCAGCGGCA AACTGTTGTC 
AGAGTATTGA ATGCAACTGG GGAAGAAATA ATTCAGCAGT CCTCAAAAAT AGATGCCAGT 
ATTCTACAAG AAAAATTGGG AAGCCTGAAT CTGCGGTGGC AGGAGGTCTG CAAACAGCTG 
GCCGAGAGAA AAAAGAGGTT AGAAGAACAA AAGAATATCC TGTCAGAATT TCAAAGAGAT 
TTGAATGAAT TTGTTTTATG GTTGGAAGAA GCAGGTAACA TTTCTAGTAT TCCTCTTGAA 
CCTGGAAATG AGCAGCAACT AAAAGAAAAG CTTGAGGAAG TCAAGTTACT GGCCGAAGAG 
TTGCCCCTGC GTCAGGGAAT TCTAAAACAA TTAAATGAAA CAGGAGGAAC AGTGCTTGTA 
AGTGCTCCCA TAAGCCCAGA AGAGCAAGAT AAACTTGAAA ATAAGCTCAA GCAGACAAAT 
CTTCAGTGGA TAAAGGTTTC TAGGAGTTTA CCTGAGAAAC AGGGAGAAAT TGAGGCTCAC 
ATAAAAGACC TTGGACAGCT TGAAGAGCAA TTAAATCATC TGCTTCTGTG GCTGTCTCCT 
ATTAGGAGTC AGTTGGAAAT TTACAACCAA CCAAATCAGA CAGGACCATT TGACATTAAG 
GAAACTGAAG TAGCAGTTCA AGCTAAACAG CCGGATGTGG AAGGGATTTT GTCTAAAGGG 
CAGAATTTGT ACAAGGAAAA ACCAGCCACT GAGCCAGTGA AGAGGAAGTT AGAAGATCTG 
AGCTCGGAGT GGAAGGCGGT AACCCACTTA CTTCAAGAGC TGAGGGCAAA GTGGCCTGGC 
CCAGTCCCTG GACTGACAGC AACTGGAGCA CCTCCTAGTC AGACGGTTGC TCTGGTGACA 
CAACCTGTGG TTGCCAAGGA AACCGCTGTC TCCAAACTAG AAATGCCATC TTCCTTGCTG 
TTGGAGGTCC CTGCACTGGC AGATTTCAAC CGGGCTTGGA CAGAACTTAC CGACTGGCTG 
TCTCTGCTTG ATCGAGTTCT GAAAGCACAG AGGGTGATGG TGGGTGATCT TGAAGACATC 
AACGAGATGA TCATCAAGCA GAAGGCAACA CTGCAGGACT TGGAGCAGAG GCGCCCCCAG 
TTGGAAGAAC TCATTACTGC TGCCCAGAAT TTGAAAAACA AGACCAGCAA TCAAGAGGCC 
AGGACCATCA TTACTGATCG AATTGAAAGA ATTCAGAGTC AGTGGGATGA AGTACAGGAA 
CACCTTCAGA ACCGGAGGCA GCAGTTAAAT GAAATGTTAA AGGATTCAAC ACAATGGCTG 
GAAGCTAAGG AAGAAGCTGA GCAGGTGGTG GGGCAGGCCA GAGCCAAGCT TGAGACATGG 
AAGGAGGGTC CCTACACAAT GGATGCAATC CAAAGGAAGA TCACAGAAAC CAAGCAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTGG CAAATGACTT GGCACTGAAA 
CTTCTCCGGG ATTATTCTGC AGATGATACC CGAAAGGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT TCATAAAAGA GTGAGTGAGC GAGAGACTGC TCTGGAAGAA 
ACTCATAGAT TACTGCAACA GTTCCCCCTG GACTTGGAGA AGTTTCTTGC CTGGCTTACA 
GAAGCTGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GACTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAGG ACCTCCAAGG AGAAATTGAA 
GCTCACACAG ATATCTATCA CAACCTAGAT GAAAATGGCC AAAAAATCCT GAGATCCCTG 
GAAGGTTCTG ATGACGCGGT CCTATTACAA AGACGTTTGG ATAATATGAA TTTCAAGTGG 
AGTGAGCTGC GGAAAAAGTC TCTCAATATC AGGTCCCATT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTGT GGCTACAGCT GAAAGACGAT 
GAGTTGAGCC GTCAGGCACC TATTGGAGGC GATTTCCCAG CAGTTCAGAA GCAGAATGAT 
ATACACAGGG CCTTCAAGAG GGAACTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTGGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCGCC TGAGGAGAAA GCCCAGAATG TCACACGACT CCTACGAAAG 
CAGGCTGAGG AGGTCAACAC AGAGTGGGAA AAATTGAACC TACACTCGGC TGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAAGAACTGC AGGAGGCCAC GGACGAGCTG 
GACCTCAAAC TGCGCCAAGC TGAGGTGATC AAGGGATCAT GGCAGCCCGT GGGCGATCTT 
CTCATTGACT CCCTCCAGGA TCACCTCGAG AAAGTCAAGG CACTTCGAGG AGAAATTGCA 
CCTCTCAAAG AGAATGTAAG CCACGTCAGT GACCTTGCTC GCCAACTCAC CACGTTGGGC 
ATTCAGCTTT CACCATATAA TCTCAGCACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CTGTTGAGGA CCGCATCAGG CAGCTGCATG AAGCCCACAG GGACTTTGGC 
CCAGCGTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCT 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCGGCTTAT 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT TTTGAGCCTG 
TCAGCCGCAT GTGATGCCTT GGACCAGCAC AACCTCAAGC AAAACGACCA GCCCATGGAC 
ATCCTGCAGA TCATTAACTG TTTGACCACT ATTTATGACC GCCTGGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GATACGGGAC GAACAGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCGC ATTTGGAAGA CAAGTACAGA TACCTTTTCA AGCAAGTGGC CAGTTCAACA 
GGATTTTGTG ACCAGCGCAG ACTGGGCCTC CTTCTACATG ACTCTATCCA AATCCCAAGA 
CAGTTGGGTG AAGTTGCATC CTTTGGGGGC AGTAACATTG AGCCAAGTGT CAGGAGCTGC 
TTCCAATTCG CTAATAATAA GCCTGAGATC GAAGCAGCTC TCTTCCTCGA TTGGATGAGA 
CTAGAACCCC AGTCCATGGT GTGGCTGCCC GTCCTGCACA GGGTGGCTGC CGCGGAGACC 
GCCAAGCATC AGGCCAAGTG TAACATCTGC AAGGAGTGTC CAATCATCGG CTTCAGGTAC 
AGAAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGCT GCTTTTTCTC TGGCCGCGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATATT GTACTCCGAC TACATCGGGA 
GAAGATGTTC GAGACTTTGC CAAGGTACTA AAAAACAAAT TTCGCACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACGG TCTTAGAGGG GGACAACATG 
GAAACGCTAG CAGAAATGGA AAACAGCAGT GGATCTTATC TAAATGATAG CATCTCTCCT 
AATGAGAGCA TAGATGATGA ACATTTGTTA ATCCAGCATT ACTGCCAAAG TTTGAACCAG 
GACTCCCCCC TGAGCCAGCC TCGTAGTCCT GCCCAGATCT TGATTTCCTT AGAGAGTGAG 
GAAAGAGGGG AGCTAGAGAG AATCCTAGCA GATCTTGAGG AAGAAAACAG GAATCTGCAG 
GCTGAATATG ATCGTCTCAA GGAGCAGCAT GAACATAAAG GCCTGTCCCC ACTGCCGTCC 
CCTCCTGAGA TGATGCCCAC CTCGCCACAG AGTCCCCGGG ATGCTGAGCT CATTGCTGAG 
GCCAAGCTAC TGCGTCAACA CAAAGGCCGC CTGGAAGCCA GGATGCAAAT CCTGGAAGAC 
CACAATAAAC AGCTGGAGTC CCAGTTACAC AGGCTCAGGC AGCTGCTGGA GCAACCCCAG 
GCTGAGGCCA AGGTGAATGG CACAACGGTG TCCTCTCCTT CTACCTCTCT TCAGAGGTCA 
GACAGCAGCC AGCCTATGCT GCTCCGGGTG GTTGGCAGTC AAACTTCAGA ATCCATGGGC 
GAGGAAGACC TTCTCAGTCC TCCCCAGGAC TCAAGCACAG GGTTAGAGGA AGTGATGGAG 
CAACTCAACA ACTCCTTCCC TAGTTCCAGA GGAAGAAATA CCCCTGGGAA GCCAATGAGA 
GAGGACACAA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_024988367.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844135.1 Bos taurus dystrophin (DMD), transcript variant X9, mRNA.

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CloneID	OBa238545
Clone ID Related Accession (Same CDS sequence)	XM_024988367.1
Accession Version	XM_024988367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATCT GGAGAGAGGA AGTAAAAGTG CTTCAAGAAG ACCTAGAACA GGAACAAGTC 
AGAGTCAATT CTCTCACGCA CATGGTGGTG GTTGTAGATG AGTCTAGTGG TGATCATGCA 
ACTGCTGCTT TGGAAGAACA ACTTAAGGTA CTGGGAGATC GGTGGGCAAA CATCTGTAGG 
TGGACTGAAG ATCGCTGGGT TCTTTTACAG GATGTTCTTC TAAAATGGCA ACGTTTTACT 
GAAGAGCAGT GCCTTTTTAG TACATGGCTT TCAGATAAAG AAGATGCATT GAACAAGATT 
CCCACAAGTG GCTTTAAGGA TCAAAGTGAA ATGTTATCAA GTCTTCAAAA ACTGGCCGTT 
TTAAAAACTG ATCTAGAAAA GAAAAAGCAA ACCATGGACA AACTCTGCTC ACTCAACCAT 
GATCTCCTTT CAACACTGAA AAATACATTG GTTGCCCAAA AGATGGAAGC ATGGTTGGAC 
AACTTTGCCC AGCGCTGGGA TAATTTAGTC CAAAAACTTG AAAAGAGTTC AACACAGATT 
TCACAGGCTG TCACCACCAC TCAGCCATCA CTAACACAGA CAACTGTAAT GGAAACGGTA 
ACTATGGTGA CCACGAGGGA GCAAATCCTG GTAAAGCATG CCCAAGAGGA ACTCCCTCCA 
CCACCTCCAC AAAAGAAGAG GCAGATTATT GTGGATTCTG AAATTAAGAA AAGGTTGGAT 
GTCGATATAA CTGAACTTCA CAGTTGGATT ACTCGTTCAG AAGCCGTGTT ACAGAGTCCT 
GAATTTGCAG TCTATCGGAA GGAAGGCAAC TTCTCAGACC TTAAAGAAAA AGTCAATGCC 
ATAGAGCGAG AAAAAGCCGA GAAGTTCAGA AAACTGCAAG ATGCCAGCAG ATCAGCTCAG 
GCCCTGGTGG AACAGATGGT GAATGAGGGT GTTAATGCTG ACAGTATCAA ACAAGCCTCA 
GAACAACTGA ACAGCCGGTG GATCGAGTTC TGCCAATTGC TAAGTGAGAG ACTTAACTGG 
CTGGAGTATC AGAACAACAT CATCACTTTC TATAATCAGC TACAACAATT GGAGCAGATG 
ACAACTACTG CAGAAAACTG GTTGAAAACT CAACCCACTA CCCCATCAGA GCCAACAGCA 
GTTAAAAGCC AGTTAAAAAA TTGTAAGGAT GAAGTCAACC GGCTGTCAGC TCTTCAGCCT 
CAAATCGAGA GATTAAAAAT TCAAAGCATA GCCCTGAAAG AGAAAGGACA AGGGCCAATG 
TTCCTGGATG CCGACTTTGT GGCCTTTACA AATCATTTTA ACCAAGTCTT TGCTGATATG 
CAGGCCAGAG AGAAAGAGTT ACAGACAATT TTGGACACTT TACCCACCGT GCGGTATCAG 
GAGACCATGA GTACCATCCT CACGTGGATC CAACAGTCAG AAACCAAACT CTCGATACCT 
CAGGTTACTG TCACTGAATA CGAGATCATG GAGCAGAGAC TTGAGGAGTT ACAGGCTTTA 
CAAAGCTCTC TGCAAGAGCA CCAAAATGAC CTAAACTATC TCAGCACCAC TGTGAAAGAG 
ATGTCAAGGA AAGCACCCTC TCATATAAGC CAGAGATATC AGTCAGAATT TGAAAACATT 
GAGGGTCGGT GGAAGAAACT CTCTGCCCAG CTGAATGAAC GTTGCCAAAA GCTAGAGGAG 
CAAATGGCCA AACTCCGAAA ACTTCAGAAT CACATAAAAA CACTGAAGAA GTGGATGGCT 
GAAGTTGATG TTTTCTTGAA AGATGAATGG CCTGCCCTTG GAGATTCAGA AATTCTTAAA 
AAACAGCTGA AGCAGTGCAG GCTTTTAGTC AGTGATATTC AGACAATCCA GCCCAGTCTA 
AACAGTGTCA ATGAAGGTGG ACAGAAAATA AAAACTGAAG CGGAGCCAGA GTTTGCTTCT 
AGACTGGAGA CAGAGCTCAG AGAACTTAAC ACTCAATGGG ATTACATATG TCGACAGGTC 
TATGCCAGAA AGGAGGCCTT AAAGGGAGGC TTGGATAAAA CTGTAAGCCT TCAGAAAGAT 
TTGTCAGAGA TGCATGAATG GATGACACAA GCCGAAGAAG AATACCTAGA GAGAGATTTT 
GAATATAAAA CCCCAGATGA ATTACAGACA GCAGTTGAAG AGATGAAGAG AGCTAAAGAA 
GAGGCCCAGC AAAAAGAAGC AAAAGTGCAG CTCCTCACTG AGTCCGTGAA CAGTGTCATA 
GCTCAAGCTC CACCTGCAGC ACAAGAGGCC TTAAGAAAGG AACTGGACAC TCTGACCACC 
AACTACCAGT GGCTCTGCAC CAGGCTGAAT GGAAAATGCA AGACGTTAGA AGAGGTTTGG 
GCATGTTGGC ATGAGTTATT GTCATACTTG GAAAAGGCAA ATAAGTGGCT AAATGAAGTA 
GAATTGAAAC TTAAGACTAC TGAAAATATT CCTGGTGGAG CTGAGGAAAT CTCTGAGGTG 
CTCAGTTCAC TTGAAAATTT GATGCAACAT TCAGAAGATA ACCCGAATCA GATTCGCATA 
TTGGCACAGA CCCTAACAGA TGGTGGAGTC ATGGATGAAC TGATCAATGA AGAACTCGAG 
ACATTTAATT CTCGTTGGAG AGAACTACAT GAAGAGGCTG TGAGGAGGCA AAAGTTGCTT 
GAACAGAGTA TCCAGTCTGC CCAGGAGATT GAAAAATCCT TACAGTTAAT TCAGGAATCC 
CTCTCATCAA TTGACAAGCA GTTGGCAGCT TATATCGCTG ACAAAGTGGA CGCCGCTCAG 
ATGCCTCAGG AAGCCCAGAA AATCCAATCT GATTTGACAA GTCATGAGAT CAGTTTAGAA 
GAAATGAAGA AACATTACCA AGGAAAGGAA ACTGCTCAAC GGGTACTATC CCAAATTGAA 
GTGGCACAGA AAAAAATGCA AGATGTTTCC ATGAAATTTC GATTGTTCCA GAAGCCAGCC 
AATTTTGAGC AGCGTCTACA GGAAAGTAAG ATGATTTTAG ATGAAGTGAA GATGCACTTG 
CCCGCACTGG AAACAAAGAG TGTTGAACAG GAAGTAGTAC AGTCACAGTT AAATCATTGT 
GTGAACTTGT ATAAAAGTCT GAGTGAAGTG AAGTCTGAAG TGGAAATGGT GATTAAAACT 
GGACGTCAAA TTGTACAGAA GAAGCAGACG GAAAACCCTA AAGAGCTTGA TGAAAGAGTA 
ACGGCTTTGA AATTGCATTA TAATGAGCTG GGAGCAAAGG TAACAGAAAG AAAGCAACAG 
TTGGAGAAAT GCTTGAAATT GTCCCGTAAG ATGCGAAAGG AAATGAATGC CTTGACAGAA 
TGGCTGGCAG CTACAGATTT GGAACTGACA AAGAGATCAG CAGTTGAAGG AATGCCTAGT 
AATTTGGATT CTGAAGTTGC CTGGGGAAAG GCTACTCAGA AAGAGACTGA GAAACAGAAG 
GTTCACCTAA AGAGCATTAC AGAGCTAGGA GAGGCCTTGA AAACGGTTTT GGGCAAGAAG 
GAGACCTTGG TCGAAGATAA ACTGAGTCTT TTGAATAGTA ACTGGATAGC CGTCACTTCT 
CGAGCAGAAG AGTGGCTAAA CCTTTTGTTG GAATACCAGA AACACATGGA AACCTTTGAC 
CAGAATGTGG ATCATATCAC AAAGTGGATC ATTCAAGCTG ATGCACTTCT GGATGAATCT 
GAGAAAAAGA AACCACAGCA AAAAGAAGAC ATGCTAAAGC GCTTAAAGGC TGAACTGAAT 
GACATACGCC CAAAGGTGGA CTCCACGCGT GACCAAGCAG CAAACTTGAT GGCAAACCGC 
GGCGACCACT GCAGGAAAGT AATAGAGCCC AAAATCTCAG AGCTCAACCA TCGATTTGCA 
GCCATTTCTC ACAGAATTAA GACTGGAAAG GCCTCCATTC CTTTGAAGGA ATTGGAGCAG 
TTTAACTCAG ATATACAAAA ATTGCTTGAA CCACTGGAGG CTGAAATTCA GCAGGGGGTG 
AATCTGAAAG AGGAAGACTT CAATAAAGAT ATGAGTGAAG ACAATGAGGG TACTGTAAAA 
GAATTGTTGC AAAGAGGAGA CAACTTACAA CAAAGAATCA CAGATGAGAG AAAGCGAGAG 
GAAATAAAGA TAAAACAGCA GCTGTTACAG ACAAAACATA ATGCTCTCAA GGATTTGAGG 
TCTCAAAGAA GAAAAAAGGC TCTAGAAATT TCTCACCAGT GGTATCAGTA CAAGAGGCAG 
GCTGATGATC TCCTGAAATG CTTGGATGAC ATTGAAAAAA AATTAGCCAG CCTACCTGAA 
CCCAGAGATG AAAGGAAAAT AAAGGAAATT GATCGTGAGT TGCAGAAGAA GAAAGAGGAG 
CTGAATGCAG TGCATAGGCA AGCTGAGGGC TTGTCTGAGG ATGGGGCAGC AATGGCAGTG 
GAGTCAACTC AGATCCAGCT CAGCAAGCGC TGGCGGGAAA TTGAGAGCAA ATTTGCTCAG 
TTTCGAAGAC TCAACTTTGC ACAAATTCAC ACTGTCCATG AAGAATCAGT GATGGTGATG 
ACCGAAGACA TGCCTTTGGA AATTTCTTAT GTGCCTTCTA CTTACCTGAC TGAGATCACT 
CACGTCTTAC AAGCCCTTTC AGAAGTGGAA CAACTTCTCA ATGCTCCCGA CCTCTGTGCT 
AAAGATTTTC AAGATCTCTT CAAACAAGAG GAATCTCTGA AGAATATAAA AGACAACCTG 
CAACAAATCT CAGGTCGGAT TGATGTTATT CACAATAAGA AGGCGGCAGC ATTGCAAAGC 
ACCACACCCC CAGAAAAGGC AAGGTTACAG GAAGCTCTCT CCAGGCTCGA CTTCCAATGG 
GAAAGAGTGA ACAAAATGTA CAAGGACCGA CAAGGGCAAT TTGACAGATC TGTTGAAAAA 
TGGCGACGGT TTCATTATGA TATGAAGATA TTTAATCAAT GGCTAACAGA AGCTGAACAC 
TTTCTCAAAA AGACACAAAT TCCTGAGAAC TGGGAACATG CCAAATATAA ATGGTATCTT 
AAGGAACTCC AGGATGGCAT CGGGCAGCGG CAAACTGTTG TCAGAGTATT GAATGCAACT 
GGGGAAGAAA TAATTCAGCA GTCCTCAAAA ATAGATGCCA GTATTCTACA AGAAAAATTG 
GGAAGCCTGA ATCTGCGGTG GCAGGAGGTC TGCAAACAGC TGGCCGAGAG AAAAAAGAGG 
TTAGAAGAAC AAAAGAATAT CCTGTCAGAA TTTCAAAGAG ATTTGAATGA ATTTGTTTTA 
TGGTTGGAAG AAGCAGGTAA CATTTCTAGT ATTCCTCTTG AACCTGGAAA TGAGCAGCAA 
CTAAAAGAAA AGCTTGAGGA AGTCAAGTTA CTGGCCGAAG AGTTGCCCCT GCGTCAGGGA 
ATTCTAAAAC AATTAAATGA AACAGGAGGA ACAGTGCTTG TAAGTGCTCC CATAAGCCCA 
GAAGAGCAAG ATAAACTTGA AAATAAGCTC AAGCAGACAA ATCTTCAGTG GATAAAGGTT 
TCTAGGAGTT TACCTGAGAA ACAGGGAGAA ATTGAGGCTC ACATAAAAGA CCTTGGACAG 
CTTGAAGAGC AATTAAATCA TCTGCTTCTG TGGCTGTCTC CTATTAGGAG TCAGTTGGAA 
ATTTACAACC AACCAAATCA GACAGGACCA TTTGACATTA AGGAAACTGA AGTAGCAGTT 
CAAGCTAAAC AGCCGGATGT GGAAGGGATT TTGTCTAAAG GGCAGAATTT GTACAAGGAA 
AAACCAGCCA CTGAGCCAGT GAAGAGGAAG TTAGAAGATC TGAGCTCGGA GTGGAAGGCG 
GTAACCCACT TACTTCAAGA GCTGAGGGCA AAGTGGCCTG GCCCAGTCCC TGGACTGACA 
GCAACTGGAG CACCTCCTAG TCAGACGGTT GCTCTGGTGA CACAACCTGT GGTTGCCAAG 
GAAACCGCTG TCTCCAAACT AGAAATGCCA TCTTCCTTGC TGTTGGAGGT CCCTGCACTG 
GCAGATTTCA ACCGGGCTTG GACAGAACTT ACCGACTGGC TGTCTCTGCT TGATCGAGTT 
CTGAAAGCAC AGAGGGTGAT GGTGGGTGAT CTTGAAGACA TCAACGAGAT GATCATCAAG 
CAGAAGGCAA CACTGCAGGA CTTGGAGCAG AGGCGCCCCC AGTTGGAAGA ACTCATTACT 
GCTGCCCAGA ATTTGAAAAA CAAGACCAGC AATCAAGAGG CCAGGACCAT CATTACTGAT 
CGAATTGAAA GAATTCAGAG TCAGTGGGAT GAAGTACAGG AACACCTTCA GAACCGGAGG 
CAGCAGTTAA ATGAAATGTT AAAGGATTCA ACACAATGGC TGGAAGCTAA GGAAGAAGCT 
GAGCAGGTGG TGGGGCAGGC CAGAGCCAAG CTTGAGACAT GGAAGGAGGG TCCCTACACA 
ATGGATGCAA TCCAAAGGAA GATCACAGAA ACCAAGCAGT TGGCCAAAGA CCTCCGCCAG 
TGGCAGATAA ATGTAGATGT GGCAAATGAC TTGGCACTGA AACTTCTCCG GGATTATTCT 
GCAGATGATA CCCGAAAGGT ACACATGATA ACAGAGAACA TCAATGCCTC TTGGGCAAGC 
ATTCATAAAA GAGTGAGTGA GCGAGAGACT GCTCTGGAAG AAACTCATAG ATTACTGCAA 
CAGTTCCCCC TGGACTTGGA GAAGTTTCTT GCCTGGCTTA CAGAAGCTGA AACAACTGCC 
AACGTCCTGC AGGATGCCAC CCATAAGGAA AGGCTTCTAG AAGACTCCAA GGGAGTAAGA 
GAGCTGATGA AACAATGGCA GGACCTCCAA GGAGAAATTG AAGCTCACAC AGATATCTAT 
CACAACCTAG ATGAAAATGG CCAAAAAATC CTGAGATCCC TGGAAGGTTC TGATGACGCG 
GTCCTATTAC AAAGACGTTT GGATAATATG AATTTCAAGT GGAGTGAGCT GCGGAAAAAG 
TCTCTCAATA TCAGGTCCCA TTTGGAAGCC AGTTCTGACC AGTGGAAGCG TCTGCACCTT 
TCTCTTCAGG AACTTCTGGT GTGGCTACAG CTGAAAGACG ATGAGTTGAG CCGTCAGGCA 
CCTATTGGAG GCGATTTCCC AGCAGTTCAG AAGCAGAATG ATATACACAG GGCCTTCAAG 
AGGGAACTGA AAACGAAAGA ACCTGTAATC ATGAGTACTC TTGAGACTGT ACGAATATTT 
CTGACAGAGC AGCCTTTGGA AGGACTAGAG AAACTCTACC AGGAGCCCAG AGAGCTGCCG 
CCTGAGGAGA AAGCCCAGAA TGTCACACGA CTCCTACGAA AGCAGGCTGA GGAGGTCAAC 
ACAGAGTGGG AAAAATTGAA CCTACACTCG GCTGACTGGC AGAGAAAAAT AGACGAGGCC 
CTCGAAAGAC TCCAAGAACT GCAGGAGGCC ACGGACGAGC TGGACCTCAA ACTGCGCCAA 
GCTGAGGTGA TCAAGGGATC ATGGCAGCCC GTGGGCGATC TTCTCATTGA CTCCCTCCAG 
GATCACCTCG AGAAAGTCAA GGCACTTCGA GGAGAAATTG CACCTCTCAA AGAGAATGTA 
AGCCACGTCA GTGACCTTGC TCGCCAACTC ACCACGTTGG GCATTCAGCT TTCACCATAT 
AATCTCAGCA CTCTGGAAGA CCTGAACACC AGATGGAAGC TTCTGCAGGT GGCTGTTGAG 
GACCGCATCA GGCAGCTGCA TGAAGCCCAC AGGGACTTTG GCCCAGCGTC CCAGCACTTC 
CTTTCCACTT CTGTCCAGGG TCCCTGGGAG AGAGCCATCT CACCAAACAA AGTGCCCTAC 
TATATCAACC ACGAGACCCA AACAACTTGC TGGGACCATC CTAAAATGAC AGAGCTCTAC 
CAGTCTTTAG CTGACCTGAA TAATGTCAGG TTCTCGGCTT ATAGGACTGC CATGAAACTC 
CGAAGACTGC AGAAGGCCCT TTGCTTGGAT CTTTTGAGCC TGTCAGCCGC ATGTGATGCC 
TTGGACCAGC ACAACCTCAA GCAAAACGAC CAGCCCATGG ACATCCTGCA GATCATTAAC 
TGTTTGACCA CTATTTATGA CCGCCTGGAG CAAGAGCACA ACAATCTGGT CAACGTCCCT 
CTCTGCGTGG ATATGTGTCT CAATTGGCTG CTGAATGTTT ATGATACGGG ACGAACAGGG 
AGGATCCGGG TCCTGTCTTT TAAAACTGGC ATCATTTCTC TGTGTAAAGC GCATTTGGAA 
GACAAGTACA GATACCTTTT CAAGCAAGTG GCCAGTTCAA CAGGATTTTG TGACCAGCGC 
AGACTGGGCC TCCTTCTACA TGACTCTATC CAAATCCCAA GACAGTTGGG TGAAGTTGCA 
TCCTTTGGGG GCAGTAACAT TGAGCCAAGT GTCAGGAGCT GCTTCCAATT CGCTAATAAT 
AAGCCTGAGA TCGAAGCAGC TCTCTTCCTC GATTGGATGA GACTAGAACC CCAGTCCATG 
GTGTGGCTGC CCGTCCTGCA CAGGGTGGCT GCCGCGGAGA CCGCCAAGCA TCAGGCCAAG 
TGTAACATCT GCAAGGAGTG TCCAATCATC GGCTTCAGGT ACAGAAGTCT AAAGCACTTT 
AATTATGACA TCTGCCAAAG CTGCTTTTTC TCTGGCCGCG TTGCAAAAGG CCATAAAATG 
CACTATCCCA TGGTGGAATA TTGTACTCCG ACTACATCGG GAGAAGATGT TCGAGACTTT 
GCCAAGGTAC TAAAAAACAA ATTTCGCACC AAAAGGTATT TTGCGAAGCA TCCCCGAATG 
GGCTACCTGC CAGTGCAGAC GGTCTTAGAG GGGGACAACA TGGAAACTCC TGTTACTCTG 
ATCAACTTCT GGCCGGTAGA CTCTGCGCCT GCCTCGTCCC CTCAGCTTTC ACACGATGAT 
ACTCATTCAC GCATTGAACA TTATGCTAGC AGGCTAGCAG AAATGGAAAA CAGCAGTGGA 
TCTTATCTAA ATGATAGCAT CTCTCCTAAT GAGAGCATAG ATGATGAACA TTTGTTAATC 
CAGCATTACT GCCAAAGTTT GAACCAGGAC TCCCCCCTGA GCCAGCCTCG TAGTCCTGCC 
CAGATCTTGA TTTCCTTAGA GAGTGAGGAA AGAGGGGAGC TAGAGAGAAT CCTAGCAGAT 
CTTGAGGAAG AAAACAGGAA TCTGCAGGCT GAATATGATC GTCTCAAGGA GCAGCATGAA 
CATAAAGGCC TGTCCCCACT GCCGTCCCCT CCTGAGATGA TGCCCACCTC GCCACAGAGT 
CCCCGGGATG CTGAGCTCAT TGCTGAGGCC AAGCTACTGC GTCAACACAA AGGCCGCCTG 
GAAGCCAGGA TGCAAATCCT GGAAGACCAC AATAAACAGC TGGAGTCCCA GTTACACAGG 
CTCAGGCAGC TGCTGGAGCA ACCCCAGGCT GAGGCCAAGG TGAATGGCAC AACGGTGTCC 
TCTCCTTCTA CCTCTCTTCA GAGGTCAGAC AGCAGCCAGC CTATGCTGCT CCGGGTGGTT 
GGCAGTCAAA CTTCAGAATC CATGGGCGAG GAAGACCTTC TCAGTCCTCC CCAGGACTCA 
AGCACAGGGT TAGAGGAAGT GATGGAGCAA CTCAACAACT CCTTCCCTAG TTCCAGAGGA 
CACAATGTAG GAAGCCTTTT CCACTTGGCA GATGATTTGG GCAGAGCGAT GGAGTCCTTA 
GTTTCAGTTA TGACAGATGA AGAAGGGGCA GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844135.1
CDS	16..9651
Translation	MKIWREEVKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDVLLKWQRFTEEQCLFSTWLSDKEDALNKIPTSGFKDQSEMLSSLQKLAVLKTDLEKKKQTMDKLCSLNHDLLSTLKNTLVAQKMEAWLDNFAQRWDNLVQKLEKSSTQISQAVTTTQPSLTQTTVMETVTMVTTREQILVKHAQEELPPPPPQKKRQIIVDSEIKKRLDVDITELHSWITRSEAVLQSPEFAVYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTPSEPTAVKSQLKNCKDEVNRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADMQAREKELQTILDTLPTVRYQETMSTILTWIQQSETKLSIPQVTVTEYEIMEQRLEELQALQSSLQEHQNDLNYLSTTVKEMSRKAPSHISQRYQSEFENIEGRWKKLSAQLNERCQKLEEQMAKLRKLQNHIKTLKKWMAEVDVFLKDEWPALGDSEILKKQLKQCRLLVSDIQTIQPSLNSVNEGGQKIKTEAEPEFASRLETELRELNTQWDYICRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVQLLTESVNSVIAQAPPAAQEALRKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLNEVELKLKTTENIPGGAEEISEVLSSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLQLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHYQGKETAQRVLSQIEVAQKKMQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDLELTKRSAVEGMPSNLDSEVAWGKATQKETEKQKVHLKSITELGEALKTVLGKKETLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMETFDQNVDHITKWIIQADALLDESEKKKPQQKEDMLKRLKAELNDIRPKVDSTRDQAANLMANRGDHCRKVIEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVHRQAEGLSEDGAAMAVESTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVMVMTEDMPLEISYVPSTYLTEITHVLQALSEVEQLLNAPDLCAKDFQDLFKQEESLKNIKDNLQQISGRIDVIHNKKAAALQSTTPPEKARLQEALSRLDFQWERVNKMYKDRQGQFDRSVEKWRRFHYDMKIFNQWLTEAEHFLKKTQIPENWEHAKYKWYLKELQDGIGQRQTVVRVLNATGEEIIQQSSKIDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDLNEFVLWLEEAGNISSIPLEPGNEQQLKEKLEEVKLLAEELPLRQGILKQLNETGGTVLVSAPISPEEQDKLENKLKQTNLQWIKVSRSLPEKQGEIEAHIKDLGQLEEQLNHLLLWLSPIRSQLEIYNQPNQTGPFDIKETEVAVQAKQPDVEGILSKGQNLYKEKPATEPVKRKLEDLSSEWKAVTHLLQELRAKWPGPVPGLTATGAPPSQTVALVTQPVVAKETAVSKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVLKAQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVVGQARAKLETWKEGPYTMDAIQRKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRVSERETALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKILRSLEGSDDAVLLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDIHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEEKAQNVTRLLRKQAEEVNTEWEKLNLHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVSHVSDLARQLTTLGIQLSPYNLSTLEDLNTRWKLLQVAVEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSSGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKEQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDSSTGLEEVMEQLNNSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_024988367.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophin (DMD), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988367.1

ORF Insert Sequence:

ATGAAAATCT GGAGAGAGGA AGTAAAAGTG CTTCAAGAAG ACCTAGAACA GGAACAAGTC 
AGAGTCAATT CTCTCACGCA CATGGTGGTG GTTGTAGATG AGTCTAGTGG TGATCATGCA 
ACTGCTGCTT TGGAAGAACA ACTTAAGGTA CTGGGAGATC GGTGGGCAAA CATCTGTAGG 
TGGACTGAAG ATCGCTGGGT TCTTTTACAG GATGTTCTTC TAAAATGGCA ACGTTTTACT 
GAAGAGCAGT GCCTTTTTAG TACATGGCTT TCAGATAAAG AAGATGCATT GAACAAGATT 
CCCACAAGTG GCTTTAAGGA TCAAAGTGAA ATGTTATCAA GTCTTCAAAA ACTGGCCGTT 
TTAAAAACTG ATCTAGAAAA GAAAAAGCAA ACCATGGACA AACTCTGCTC ACTCAACCAT 
GATCTCCTTT CAACACTGAA AAATACATTG GTTGCCCAAA AGATGGAAGC ATGGTTGGAC 
AACTTTGCCC AGCGCTGGGA TAATTTAGTC CAAAAACTTG AAAAGAGTTC AACACAGATT 
TCACAGGCTG TCACCACCAC TCAGCCATCA CTAACACAGA CAACTGTAAT GGAAACGGTA 
ACTATGGTGA CCACGAGGGA GCAAATCCTG GTAAAGCATG CCCAAGAGGA ACTCCCTCCA 
CCACCTCCAC AAAAGAAGAG GCAGATTATT GTGGATTCTG AAATTAAGAA AAGGTTGGAT 
GTCGATATAA CTGAACTTCA CAGTTGGATT ACTCGTTCAG AAGCCGTGTT ACAGAGTCCT 
GAATTTGCAG TCTATCGGAA GGAAGGCAAC TTCTCAGACC TTAAAGAAAA AGTCAATGCC 
ATAGAGCGAG AAAAAGCCGA GAAGTTCAGA AAACTGCAAG ATGCCAGCAG ATCAGCTCAG 
GCCCTGGTGG AACAGATGGT GAATGAGGGT GTTAATGCTG ACAGTATCAA ACAAGCCTCA 
GAACAACTGA ACAGCCGGTG GATCGAGTTC TGCCAATTGC TAAGTGAGAG ACTTAACTGG 
CTGGAGTATC AGAACAACAT CATCACTTTC TATAATCAGC TACAACAATT GGAGCAGATG 
ACAACTACTG CAGAAAACTG GTTGAAAACT CAACCCACTA CCCCATCAGA GCCAACAGCA 
GTTAAAAGCC AGTTAAAAAA TTGTAAGGAT GAAGTCAACC GGCTGTCAGC TCTTCAGCCT 
CAAATCGAGA GATTAAAAAT TCAAAGCATA GCCCTGAAAG AGAAAGGACA AGGGCCAATG 
TTCCTGGATG CCGACTTTGT GGCCTTTACA AATCATTTTA ACCAAGTCTT TGCTGATATG 
CAGGCCAGAG AGAAAGAGTT ACAGACAATT TTGGACACTT TACCCACCGT GCGGTATCAG 
GAGACCATGA GTACCATCCT CACGTGGATC CAACAGTCAG AAACCAAACT CTCGATACCT 
CAGGTTACTG TCACTGAATA CGAGATCATG GAGCAGAGAC TTGAGGAGTT ACAGGCTTTA 
CAAAGCTCTC TGCAAGAGCA CCAAAATGAC CTAAACTATC TCAGCACCAC TGTGAAAGAG 
ATGTCAAGGA AAGCACCCTC TCATATAAGC CAGAGATATC AGTCAGAATT TGAAAACATT 
GAGGGTCGGT GGAAGAAACT CTCTGCCCAG CTGAATGAAC GTTGCCAAAA GCTAGAGGAG 
CAAATGGCCA AACTCCGAAA ACTTCAGAAT CACATAAAAA CACTGAAGAA GTGGATGGCT 
GAAGTTGATG TTTTCTTGAA AGATGAATGG CCTGCCCTTG GAGATTCAGA AATTCTTAAA 
AAACAGCTGA AGCAGTGCAG GCTTTTAGTC AGTGATATTC AGACAATCCA GCCCAGTCTA 
AACAGTGTCA ATGAAGGTGG ACAGAAAATA AAAACTGAAG CGGAGCCAGA GTTTGCTTCT 
AGACTGGAGA CAGAGCTCAG AGAACTTAAC ACTCAATGGG ATTACATATG TCGACAGGTC 
TATGCCAGAA AGGAGGCCTT AAAGGGAGGC TTGGATAAAA CTGTAAGCCT TCAGAAAGAT 
TTGTCAGAGA TGCATGAATG GATGACACAA GCCGAAGAAG AATACCTAGA GAGAGATTTT 
GAATATAAAA CCCCAGATGA ATTACAGACA GCAGTTGAAG AGATGAAGAG AGCTAAAGAA 
GAGGCCCAGC AAAAAGAAGC AAAAGTGCAG CTCCTCACTG AGTCCGTGAA CAGTGTCATA 
GCTCAAGCTC CACCTGCAGC ACAAGAGGCC TTAAGAAAGG AACTGGACAC TCTGACCACC 
AACTACCAGT GGCTCTGCAC CAGGCTGAAT GGAAAATGCA AGACGTTAGA AGAGGTTTGG 
GCATGTTGGC ATGAGTTATT GTCATACTTG GAAAAGGCAA ATAAGTGGCT AAATGAAGTA 
GAATTGAAAC TTAAGACTAC TGAAAATATT CCTGGTGGAG CTGAGGAAAT CTCTGAGGTG 
CTCAGTTCAC TTGAAAATTT GATGCAACAT TCAGAAGATA ACCCGAATCA GATTCGCATA 
TTGGCACAGA CCCTAACAGA TGGTGGAGTC ATGGATGAAC TGATCAATGA AGAACTCGAG 
ACATTTAATT CTCGTTGGAG AGAACTACAT GAAGAGGCTG TGAGGAGGCA AAAGTTGCTT 
GAACAGAGTA TCCAGTCTGC CCAGGAGATT GAAAAATCCT TACAGTTAAT TCAGGAATCC 
CTCTCATCAA TTGACAAGCA GTTGGCAGCT TATATCGCTG ACAAAGTGGA CGCCGCTCAG 
ATGCCTCAGG AAGCCCAGAA AATCCAATCT GATTTGACAA GTCATGAGAT CAGTTTAGAA 
GAAATGAAGA AACATTACCA AGGAAAGGAA ACTGCTCAAC GGGTACTATC CCAAATTGAA 
GTGGCACAGA AAAAAATGCA AGATGTTTCC ATGAAATTTC GATTGTTCCA GAAGCCAGCC 
AATTTTGAGC AGCGTCTACA GGAAAGTAAG ATGATTTTAG ATGAAGTGAA GATGCACTTG 
CCCGCACTGG AAACAAAGAG TGTTGAACAG GAAGTAGTAC AGTCACAGTT AAATCATTGT 
GTGAACTTGT ATAAAAGTCT GAGTGAAGTG AAGTCTGAAG TGGAAATGGT GATTAAAACT 
GGACGTCAAA TTGTACAGAA GAAGCAGACG GAAAACCCTA AAGAGCTTGA TGAAAGAGTA 
ACGGCTTTGA AATTGCATTA TAATGAGCTG GGAGCAAAGG TAACAGAAAG AAAGCAACAG 
TTGGAGAAAT GCTTGAAATT GTCCCGTAAG ATGCGAAAGG AAATGAATGC CTTGACAGAA 
TGGCTGGCAG CTACAGATTT GGAACTGACA AAGAGATCAG CAGTTGAAGG AATGCCTAGT 
AATTTGGATT CTGAAGTTGC CTGGGGAAAG GCTACTCAGA AAGAGACTGA GAAACAGAAG 
GTTCACCTAA AGAGCATTAC AGAGCTAGGA GAGGCCTTGA AAACGGTTTT GGGCAAGAAG 
GAGACCTTGG TCGAAGATAA ACTGAGTCTT TTGAATAGTA ACTGGATAGC CGTCACTTCT 
CGAGCAGAAG AGTGGCTAAA CCTTTTGTTG GAATACCAGA AACACATGGA AACCTTTGAC 
CAGAATGTGG ATCATATCAC AAAGTGGATC ATTCAAGCTG ATGCACTTCT GGATGAATCT 
GAGAAAAAGA AACCACAGCA AAAAGAAGAC ATGCTAAAGC GCTTAAAGGC TGAACTGAAT 
GACATACGCC CAAAGGTGGA CTCCACGCGT GACCAAGCAG CAAACTTGAT GGCAAACCGC 
GGCGACCACT GCAGGAAAGT AATAGAGCCC AAAATCTCAG AGCTCAACCA TCGATTTGCA 
GCCATTTCTC ACAGAATTAA GACTGGAAAG GCCTCCATTC CTTTGAAGGA ATTGGAGCAG 
TTTAACTCAG ATATACAAAA ATTGCTTGAA CCACTGGAGG CTGAAATTCA GCAGGGGGTG 
AATCTGAAAG AGGAAGACTT CAATAAAGAT ATGAGTGAAG ACAATGAGGG TACTGTAAAA 
GAATTGTTGC AAAGAGGAGA CAACTTACAA CAAAGAATCA CAGATGAGAG AAAGCGAGAG 
GAAATAAAGA TAAAACAGCA GCTGTTACAG ACAAAACATA ATGCTCTCAA GGATTTGAGG 
TCTCAAAGAA GAAAAAAGGC TCTAGAAATT TCTCACCAGT GGTATCAGTA CAAGAGGCAG 
GCTGATGATC TCCTGAAATG CTTGGATGAC ATTGAAAAAA AATTAGCCAG CCTACCTGAA 
CCCAGAGATG AAAGGAAAAT AAAGGAAATT GATCGTGAGT TGCAGAAGAA GAAAGAGGAG 
CTGAATGCAG TGCATAGGCA AGCTGAGGGC TTGTCTGAGG ATGGGGCAGC AATGGCAGTG 
GAGTCAACTC AGATCCAGCT CAGCAAGCGC TGGCGGGAAA TTGAGAGCAA ATTTGCTCAG 
TTTCGAAGAC TCAACTTTGC ACAAATTCAC ACTGTCCATG AAGAATCAGT GATGGTGATG 
ACCGAAGACA TGCCTTTGGA AATTTCTTAT GTGCCTTCTA CTTACCTGAC TGAGATCACT 
CACGTCTTAC AAGCCCTTTC AGAAGTGGAA CAACTTCTCA ATGCTCCCGA CCTCTGTGCT 
AAAGATTTTC AAGATCTCTT CAAACAAGAG GAATCTCTGA AGAATATAAA AGACAACCTG 
CAACAAATCT CAGGTCGGAT TGATGTTATT CACAATAAGA AGGCGGCAGC ATTGCAAAGC 
ACCACACCCC CAGAAAAGGC AAGGTTACAG GAAGCTCTCT CCAGGCTCGA CTTCCAATGG 
GAAAGAGTGA ACAAAATGTA CAAGGACCGA CAAGGGCAAT TTGACAGATC TGTTGAAAAA 
TGGCGACGGT TTCATTATGA TATGAAGATA TTTAATCAAT GGCTAACAGA AGCTGAACAC 
TTTCTCAAAA AGACACAAAT TCCTGAGAAC TGGGAACATG CCAAATATAA ATGGTATCTT 
AAGGAACTCC AGGATGGCAT CGGGCAGCGG CAAACTGTTG TCAGAGTATT GAATGCAACT 
GGGGAAGAAA TAATTCAGCA GTCCTCAAAA ATAGATGCCA GTATTCTACA AGAAAAATTG 
GGAAGCCTGA ATCTGCGGTG GCAGGAGGTC TGCAAACAGC TGGCCGAGAG AAAAAAGAGG 
TTAGAAGAAC AAAAGAATAT CCTGTCAGAA TTTCAAAGAG ATTTGAATGA ATTTGTTTTA 
TGGTTGGAAG AAGCAGGTAA CATTTCTAGT ATTCCTCTTG AACCTGGAAA TGAGCAGCAA 
CTAAAAGAAA AGCTTGAGGA AGTCAAGTTA CTGGCCGAAG AGTTGCCCCT GCGTCAGGGA 
ATTCTAAAAC AATTAAATGA AACAGGAGGA ACAGTGCTTG TAAGTGCTCC CATAAGCCCA 
GAAGAGCAAG ATAAACTTGA AAATAAGCTC AAGCAGACAA ATCTTCAGTG GATAAAGGTT 
TCTAGGAGTT TACCTGAGAA ACAGGGAGAA ATTGAGGCTC ACATAAAAGA CCTTGGACAG 
CTTGAAGAGC AATTAAATCA TCTGCTTCTG TGGCTGTCTC CTATTAGGAG TCAGTTGGAA 
ATTTACAACC AACCAAATCA GACAGGACCA TTTGACATTA AGGAAACTGA AGTAGCAGTT 
CAAGCTAAAC AGCCGGATGT GGAAGGGATT TTGTCTAAAG GGCAGAATTT GTACAAGGAA 
AAACCAGCCA CTGAGCCAGT GAAGAGGAAG TTAGAAGATC TGAGCTCGGA GTGGAAGGCG 
GTAACCCACT TACTTCAAGA GCTGAGGGCA AAGTGGCCTG GCCCAGTCCC TGGACTGACA 
GCAACTGGAG CACCTCCTAG TCAGACGGTT GCTCTGGTGA CACAACCTGT GGTTGCCAAG 
GAAACCGCTG TCTCCAAACT AGAAATGCCA TCTTCCTTGC TGTTGGAGGT CCCTGCACTG 
GCAGATTTCA ACCGGGCTTG GACAGAACTT ACCGACTGGC TGTCTCTGCT TGATCGAGTT 
CTGAAAGCAC AGAGGGTGAT GGTGGGTGAT CTTGAAGACA TCAACGAGAT GATCATCAAG 
CAGAAGGCAA CACTGCAGGA CTTGGAGCAG AGGCGCCCCC AGTTGGAAGA ACTCATTACT 
GCTGCCCAGA ATTTGAAAAA CAAGACCAGC AATCAAGAGG CCAGGACCAT CATTACTGAT 
CGAATTGAAA GAATTCAGAG TCAGTGGGAT GAAGTACAGG AACACCTTCA GAACCGGAGG 
CAGCAGTTAA ATGAAATGTT AAAGGATTCA ACACAATGGC TGGAAGCTAA GGAAGAAGCT 
GAGCAGGTGG TGGGGCAGGC CAGAGCCAAG CTTGAGACAT GGAAGGAGGG TCCCTACACA 
ATGGATGCAA TCCAAAGGAA GATCACAGAA ACCAAGCAGT TGGCCAAAGA CCTCCGCCAG 
TGGCAGATAA ATGTAGATGT GGCAAATGAC TTGGCACTGA AACTTCTCCG GGATTATTCT 
GCAGATGATA CCCGAAAGGT ACACATGATA ACAGAGAACA TCAATGCCTC TTGGGCAAGC 
ATTCATAAAA GAGTGAGTGA GCGAGAGACT GCTCTGGAAG AAACTCATAG ATTACTGCAA 
CAGTTCCCCC TGGACTTGGA GAAGTTTCTT GCCTGGCTTA CAGAAGCTGA AACAACTGCC 
AACGTCCTGC AGGATGCCAC CCATAAGGAA AGGCTTCTAG AAGACTCCAA GGGAGTAAGA 
GAGCTGATGA AACAATGGCA GGACCTCCAA GGAGAAATTG AAGCTCACAC AGATATCTAT 
CACAACCTAG ATGAAAATGG CCAAAAAATC CTGAGATCCC TGGAAGGTTC TGATGACGCG 
GTCCTATTAC AAAGACGTTT GGATAATATG AATTTCAAGT GGAGTGAGCT GCGGAAAAAG 
TCTCTCAATA TCAGGTCCCA TTTGGAAGCC AGTTCTGACC AGTGGAAGCG TCTGCACCTT 
TCTCTTCAGG AACTTCTGGT GTGGCTACAG CTGAAAGACG ATGAGTTGAG CCGTCAGGCA 
CCTATTGGAG GCGATTTCCC AGCAGTTCAG AAGCAGAATG ATATACACAG GGCCTTCAAG 
AGGGAACTGA AAACGAAAGA ACCTGTAATC ATGAGTACTC TTGAGACTGT ACGAATATTT 
CTGACAGAGC AGCCTTTGGA AGGACTAGAG AAACTCTACC AGGAGCCCAG AGAGCTGCCG 
CCTGAGGAGA AAGCCCAGAA TGTCACACGA CTCCTACGAA AGCAGGCTGA GGAGGTCAAC 
ACAGAGTGGG AAAAATTGAA CCTACACTCG GCTGACTGGC AGAGAAAAAT AGACGAGGCC 
CTCGAAAGAC TCCAAGAACT GCAGGAGGCC ACGGACGAGC TGGACCTCAA ACTGCGCCAA 
GCTGAGGTGA TCAAGGGATC ATGGCAGCCC GTGGGCGATC TTCTCATTGA CTCCCTCCAG 
GATCACCTCG AGAAAGTCAA GGCACTTCGA GGAGAAATTG CACCTCTCAA AGAGAATGTA 
AGCCACGTCA GTGACCTTGC TCGCCAACTC ACCACGTTGG GCATTCAGCT TTCACCATAT 
AATCTCAGCA CTCTGGAAGA CCTGAACACC AGATGGAAGC TTCTGCAGGT GGCTGTTGAG 
GACCGCATCA GGCAGCTGCA TGAAGCCCAC AGGGACTTTG GCCCAGCGTC CCAGCACTTC 
CTTTCCACTT CTGTCCAGGG TCCCTGGGAG AGAGCCATCT CACCAAACAA AGTGCCCTAC 
TATATCAACC ACGAGACCCA AACAACTTGC TGGGACCATC CTAAAATGAC AGAGCTCTAC 
CAGTCTTTAG CTGACCTGAA TAATGTCAGG TTCTCGGCTT ATAGGACTGC CATGAAACTC 
CGAAGACTGC AGAAGGCCCT TTGCTTGGAT CTTTTGAGCC TGTCAGCCGC ATGTGATGCC 
TTGGACCAGC ACAACCTCAA GCAAAACGAC CAGCCCATGG ACATCCTGCA GATCATTAAC 
TGTTTGACCA CTATTTATGA CCGCCTGGAG CAAGAGCACA ACAATCTGGT CAACGTCCCT 
CTCTGCGTGG ATATGTGTCT CAATTGGCTG CTGAATGTTT ATGATACGGG ACGAACAGGG 
AGGATCCGGG TCCTGTCTTT TAAAACTGGC ATCATTTCTC TGTGTAAAGC GCATTTGGAA 
GACAAGTACA GATACCTTTT CAAGCAAGTG GCCAGTTCAA CAGGATTTTG TGACCAGCGC 
AGACTGGGCC TCCTTCTACA TGACTCTATC CAAATCCCAA GACAGTTGGG TGAAGTTGCA 
TCCTTTGGGG GCAGTAACAT TGAGCCAAGT GTCAGGAGCT GCTTCCAATT CGCTAATAAT 
AAGCCTGAGA TCGAAGCAGC TCTCTTCCTC GATTGGATGA GACTAGAACC CCAGTCCATG 
GTGTGGCTGC CCGTCCTGCA CAGGGTGGCT GCCGCGGAGA CCGCCAAGCA TCAGGCCAAG 
TGTAACATCT GCAAGGAGTG TCCAATCATC GGCTTCAGGT ACAGAAGTCT AAAGCACTTT 
AATTATGACA TCTGCCAAAG CTGCTTTTTC TCTGGCCGCG TTGCAAAAGG CCATAAAATG 
CACTATCCCA TGGTGGAATA TTGTACTCCG ACTACATCGG GAGAAGATGT TCGAGACTTT 
GCCAAGGTAC TAAAAAACAA ATTTCGCACC AAAAGGTATT TTGCGAAGCA TCCCCGAATG 
GGCTACCTGC CAGTGCAGAC GGTCTTAGAG GGGGACAACA TGGAAACTCC TGTTACTCTG 
ATCAACTTCT GGCCGGTAGA CTCTGCGCCT GCCTCGTCCC CTCAGCTTTC ACACGATGAT 
ACTCATTCAC GCATTGAACA TTATGCTAGC AGGCTAGCAG AAATGGAAAA CAGCAGTGGA 
TCTTATCTAA ATGATAGCAT CTCTCCTAAT GAGAGCATAG ATGATGAACA TTTGTTAATC 
CAGCATTACT GCCAAAGTTT GAACCAGGAC TCCCCCCTGA GCCAGCCTCG TAGTCCTGCC 
CAGATCTTGA TTTCCTTAGA GAGTGAGGAA AGAGGGGAGC TAGAGAGAAT CCTAGCAGAT 
CTTGAGGAAG AAAACAGGAA TCTGCAGGCT GAATATGATC GTCTCAAGGA GCAGCATGAA 
CATAAAGGCC TGTCCCCACT GCCGTCCCCT CCTGAGATGA TGCCCACCTC GCCACAGAGT 
CCCCGGGATG CTGAGCTCAT TGCTGAGGCC AAGCTACTGC GTCAACACAA AGGCCGCCTG 
GAAGCCAGGA TGCAAATCCT GGAAGACCAC AATAAACAGC TGGAGTCCCA GTTACACAGG 
CTCAGGCAGC TGCTGGAGCA ACCCCAGGCT GAGGCCAAGG TGAATGGCAC AACGGTGTCC 
TCTCCTTCTA CCTCTCTTCA GAGGTCAGAC AGCAGCCAGC CTATGCTGCT CCGGGTGGTT 
GGCAGTCAAA CTTCAGAATC CATGGGCGAG GAAGACCTTC TCAGTCCTCC CCAGGACTCA 
AGCACAGGGT TAGAGGAAGT GATGGAGCAA CTCAACAACT CCTTCCCTAG TTCCAGAGGA 
CACAATGTAG GAAGCCTTTT CCACTTGGCA GATGATTTGG GCAGAGCGAT GGAGTCCTTA 
GTTTCAGTTA TGACAGATGA AGAAGGGGCA GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DMD
Entrez Gene ID	537655
Full Name	dystrophin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Bos taurus (cattle)	LOC537655	XP_002700291.2
	DMD	XP_001096514.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DMD cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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