ADGRF5 cDNA ORF clone, Bos taurus(cattle)

The following ADGRF5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADGRF5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa229509 XM_024983638.1
Latest version!
Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa37037 NM_001193243.1
Latest version!
Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa32021 XM_005223454.4
Latest version!
Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa32021 XM_005223453.3
Latest version!
Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa32022 XM_024983637.1
Latest version!
Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa32022 XM_005223455.3
Latest version!
Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa32023 XM_010818206.2
Latest version!
Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa32024 XM_005223457.3
Latest version!
Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa32021 XM_005223453.2 Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa32023 XM_010818206.1 Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa32024 XM_005223457.2 Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa32022 XM_005223455.2 Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa32021 XM_005223454.3 Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa229509
    Clone ID Related Accession (Same CDS sequence) XM_024983638.1
    Accession Version XM_024983638.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4020bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor F5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGCAGCC
    CCAAGGGCTC CCCCAGGCAA TGATGGAAAC CCTGTTAGCC GTGGCAGTCC GCCCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839406.1
    CDS439..4458
    Translation

    Target ORF information:

    RefSeq Version XM_024983638.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983638.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGCAGCC
    CCAAGGGCTC CCCCAGGCAA TGATGGAAAC CCTGTTAGCC GTGGCAGTCC GCCCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37037
    Clone ID Related Accession (Same CDS sequence) NM_001193243.1
    Accession Version NM_001193243.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor F5
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000023.1. On Jul 17, 2010 this sequence version replaced XM_878913.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.26817.1, SRR5571258.1439.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGATATT ATCCTGAGAA TGTCGGTCAG ACTCAATGTG
    GGCTTTCAAG AAGACCTCAA GAACTCTTCC TCTGCCCTCT ATAAGTCCTA CAAGACTGAC
    CTGGAAACAG CGTTCTGGAA GGGTTACAGT ATTTTACCAG GCTTCAAATT GGTGACGGTG
    ACAGGGTTCA GGCCTGGAAG TGTGGTTGTG ACGTACGAAA TCACAACAAC AACAACAGCA
    TCACCTGCGT TAATGCGTAA AGCGAACGAG AAAGTCACAC AGAACCTCAA TCAGACCTAC
    AAGATGGAGA GCAGTTCCTA TCAGGTTACT TACAGCAATG AAACGAAATT CTCCATCACA
    CCAGAAATCA TCTTTGAAGG AGATACAGTA ACCCTGATGT GTGAGAATGA AGTTTTTTCC
    ACCAATGTGT CCTGGACGCA GGGGGAAACG GGCCTCGACA TCCAGAACAG CACTGAAGTC
    TCCATCTACA CCTCTGTGCT CAACAACATG ACCTCCGTGT CGCGCCTTAT TATCCACAAC
    ATCACTCGGG GTTTCATGGG TGAATACATC TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GCGTCCAAAA GAAAAGTGGA AGTTATACCC ATCCAGATAC TGGCAAGCGA AGCCACGGAG
    GTGATGTGTG ACAACAATCC TGTGTCTTTG CACTGCTGCA GTGAGGTGAA CGTGAATTGG
    AGTCAAATTG AATGGAAGCA GGAAGGGAGT ATAAGCGTTC CAGGAAACGC TGAAATAGAC
    ACTGACAGTG GCTGCAGCAG ATACACCCTC GTGGCCAATG GGACTCAGTG TCCGAGCGGG
    TCGTCTGGAA CGACAGTGGT CTACACGTGT GAGTTCATCA GTGCCCACGG AGCCAGAGGC
    AGAAAGGACA TAGAAGTGAC ATTCATGTCT GTGGCCAAGC TAAACATAAC CCCGGACCTA
    ATTTCTGTTT CTGAGGGACA AAGCTTTTTT ATAAAGTGCA TCAGCGATAT GAGTAACTAT
    GATGAGATGT ACTGGAACAC TTCTGCTGGA ACTAAAATAT ACAAAAGGTT TTATACCACG
    AGGAGGTATC CTGACAGAGC AGAGTCGGTG CTGACAGTCA AGACCTCAAC CAGGGAGTGG
    AATGGAACCT ATCACTGTGT ATTTCGATAC AAGAATTTGT ACAGGGTCAT GGCCAAAGAT
    GTCATCGTGT ACCCGCTGCC TCTAGAGTCC AACATCATGG TTGATCCTGT GGAAGCTGCA
    ATTCCATGCC ATAGCTCCCA TCACATCAGA TGCTGCATTG AGGAAGATTC AGACTACAGG
    GTTATTTTTC AAGTGGGTTC CTCGTCCTTT CCTGCTGTAA AAGAAGTTAA TGAAAAGCAA
    GTGTGCTACA AACACCATTT CGTGGCAGAC TCGGTTTCCT GTCCAGAAAA TGTGGATGTG
    TTCTGTCACT TTGCCAATGC CGCTAATAAT TCAGTCCAGA GCCCGTCTAT GAAGCTTAAC
    CTGGTTCCTG GGGAGAGCAT CACTTGCCAG GATCCTATCC TAGGTGTTGG GGAGCCTGGG
    AAAGTCATCC AGAAACTTTG CCGGTTCTCG AACATTACCA GAAGCCGTGA GAGTCCTATT
    GGAGGGATCA TAACTTATAG ATGCGTGGGC TCCCAGTGGC AGATTGAGAG AAATGACTGC
    ATCTCTGCTC CAATAAATGC TCTGCTCCAG CTGGCCAAGG CTTTGGTTAA GAGCCCCACC
    CAGGACGAGA AGCTCCCGTC CTACCTAGGG AATCTTTCCA TTAGCACAAA GCAAGTGAAA
    CTTGAAGTCG ACTCATTTTC TGGGACCCTG GGAGCTATTA TTAACATCCT GGACCTGTTC
    TCAACAGTTC CAACCCAAGT GAATTCAGAA ATGATGACGC ACGTCCTCTC CACAGTCAAT
    ATCATCCTTG GCAAGTCTGC CTTGAATACC TGGAAGATGT CACAGCAGCA AAAAACCAAT
    TATAGCTCAC AGCTATTGGA TTCAGTGGAA AGATTTTCCC GAGTTTTACG AGCAGAAGAC
    AGCACCATCT CCCAACCTAA CGTGCAGATG AAGAGCATGG TCATAAAACC TGGCCACCCC
    CAGTCGTACA GGCAGAGTTT TATTTTCTTG GACTCTGACC TCTGGGGCAA CGTGACCATC
    AATGGATGCC AGCTGGAACA CCTGCAGCCA GATTCATTTG TTGTCACTGT GGCTTTCCCA
    ACTCTCAAAA CCATCCTCGA CAGGGATGTT CCGGGACAGA ACTTTGCCAA CAGCTTGGTG
    ATGACCACCA CCGTCAGCCG CAGCATAACC ACGCCATTCA GGATTCTGAT GACTTTCAAA
    AACAACCACC CTTTGGGCGG GATACCGCAG TGTGTCTTCT GGAACTTCGA CCTCGCCAAC
    CACACCGGGG GGTGGGACAG CAGTGGGTGC TATGTGAAAG AGGTCACTGA GGACAGCGTG
    TCCTGCAGCT GTGAACACCT CACCTCCTTC TCCATCCTCA TGTCCCCCGA CTCCCCAGAC
    CCTGATTCTC TCCTGAAAAT CCTGCTGGAT ATCATTTCCT ACATCGGGCT GTGCTTTTCC
    ATCTTGAGCT TAGCAGCCTG CCTCGTCGTG GAGGCCGTGG TATGGAAATC GGTGACCAAG
    AACCGGACCT CCTCCATGCG CCACATCTGC ATCGTCAACA TCACTGCTTC CCTGCTGGTG
    GCCGACGTCT GGTTCATCGT GGCCGCCGCC ATCCACGACC ATCGTTTCCC GCTCAACGAG
    ACGGCCTGTG TGGCCGCCAC CTTCTTCGTC CACTTCTTCT ACCTCAGTGT CTTCTTCTGG
    ATGCTGACCC TGGGGCTCAT GCTCTTCTAC CGCCTGGTTT TCATTCTACA CGACACGAGC
    AAGTCCATTC AGAAGACGAT CGCCTTTTCT CTGGGGTACG GCTGCCCTCT GGTCATCTCC
    ATCATCACAG TGGGGGCCAC CCAGCCCCGG GAGGTCTACA TGAGGAAGAA CGCCTGCTGG
    CTCAACTGGG AGGACACCAA AGCCCTGCTG GCCTTCGTCA TCCCGGCCCT GATCATCGTG
    GTGGTAAACA TGACCATCAC GGTCGTGGTC ATCACCAAGA TCCTGAGGCC TTCCGTCGGG
    GACAAGCCGA GCAAGCAGGA GAAGAGCAGC CTGTTCCAGG TCGCCAAGAG CATTGGGGTC
    CTCACGCCGC TCTTGGGGCT CACCTGGGGT TTCGGGCTGG CCACCGTGTT CCAGGGGAGC
    AACGCTGTGT TCCACATTGT GTTCACCCTC CTCAATGCCT TCCAGGGATT ATTCATTTTA
    CTCTTTGGAT GCCTCTGGGA TCAGAAGGTG CAGGAAGCCT TGCTGAACAA GTATTCACGG
    TCAAAATGGT CTTCACAGCA CTCAAAGTCA ACGTCCATAG GTTCATCTAC ACCTGTATTT
    TCAATGAGTT CTCCAATATC AAGAAGATTT AACAATTTGT TTGGTAAAAC AGGAACATAC
    AATGTTTCCA CCCCGGAAAC AACCAGTTCA TCCCTGGAAA ACACATCCAG TGCTTATTCA
    TTGCTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180172.1
    CDS251..4282
    Translation

    Target ORF information:

    RefSeq Version NM_001193243.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193243.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGATATT ATCCTGAGAA TGTCGGTCAG ACTCAATGTG
    GGCTTTCAAG AAGACCTCAA GAACTCTTCC TCTGCCCTCT ATAAGTCCTA CAAGACTGAC
    CTGGAAACAG CGTTCTGGAA GGGTTACAGT ATTTTACCAG GCTTCAAATT GGTGACGGTG
    ACAGGGTTCA GGCCTGGAAG TGTGGTTGTG ACGTACGAAA TCACAACAAC AACAACAGCA
    TCACCTGCGT TAATGCGTAA AGCGAACGAG AAAGTCACAC AGAACCTCAA TCAGACCTAC
    AAGATGGAGA GCAGTTCCTA TCAGGTTACT TACAGCAATG AAACGAAATT CTCCATCACA
    CCAGAAATCA TCTTTGAAGG AGATACAGTA ACCCTGATGT GTGAGAATGA AGTTTTTTCC
    ACCAATGTGT CCTGGACGCA GGGGGAAACG GGCCTCGACA TCCAGAACAG CACTGAAGTC
    TCCATCTACA CCTCTGTGCT CAACAACATG ACCTCCGTGT CGCGCCTTAT TATCCACAAC
    ATCACTCGGG GTTTCATGGG TGAATACATC TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GCGTCCAAAA GAAAAGTGGA AGTTATACCC ATCCAGATAC TGGCAAGCGA AGCCACGGAG
    GTGATGTGTG ACAACAATCC TGTGTCTTTG CACTGCTGCA GTGAGGTGAA CGTGAATTGG
    AGTCAAATTG AATGGAAGCA GGAAGGGAGT ATAAGCGTTC CAGGAAACGC TGAAATAGAC
    ACTGACAGTG GCTGCAGCAG ATACACCCTC GTGGCCAATG GGACTCAGTG TCCGAGCGGG
    TCGTCTGGAA CGACAGTGGT CTACACGTGT GAGTTCATCA GTGCCCACGG AGCCAGAGGC
    AGAAAGGACA TAGAAGTGAC ATTCATGTCT GTGGCCAAGC TAAACATAAC CCCGGACCTA
    ATTTCTGTTT CTGAGGGACA AAGCTTTTTT ATAAAGTGCA TCAGCGATAT GAGTAACTAT
    GATGAGATGT ACTGGAACAC TTCTGCTGGA ACTAAAATAT ACAAAAGGTT TTATACCACG
    AGGAGGTATC CTGACAGAGC AGAGTCGGTG CTGACAGTCA AGACCTCAAC CAGGGAGTGG
    AATGGAACCT ATCACTGTGT ATTTCGATAC AAGAATTTGT ACAGGGTCAT GGCCAAAGAT
    GTCATCGTGT ACCCGCTGCC TCTAGAGTCC AACATCATGG TTGATCCTGT GGAAGCTGCA
    ATTCCATGCC ATAGCTCCCA TCACATCAGA TGCTGCATTG AGGAAGATTC AGACTACAGG
    GTTATTTTTC AAGTGGGTTC CTCGTCCTTT CCTGCTGTAA AAGAAGTTAA TGAAAAGCAA
    GTGTGCTACA AACACCATTT CGTGGCAGAC TCGGTTTCCT GTCCAGAAAA TGTGGATGTG
    TTCTGTCACT TTGCCAATGC CGCTAATAAT TCAGTCCAGA GCCCGTCTAT GAAGCTTAAC
    CTGGTTCCTG GGGAGAGCAT CACTTGCCAG GATCCTATCC TAGGTGTTGG GGAGCCTGGG
    AAAGTCATCC AGAAACTTTG CCGGTTCTCG AACATTACCA GAAGCCGTGA GAGTCCTATT
    GGAGGGATCA TAACTTATAG ATGCGTGGGC TCCCAGTGGC AGATTGAGAG AAATGACTGC
    ATCTCTGCTC CAATAAATGC TCTGCTCCAG CTGGCCAAGG CTTTGGTTAA GAGCCCCACC
    CAGGACGAGA AGCTCCCGTC CTACCTAGGG AATCTTTCCA TTAGCACAAA GCAAGTGAAA
    CTTGAAGTCG ACTCATTTTC TGGGACCCTG GGAGCTATTA TTAACATCCT GGACCTGTTC
    TCAACAGTTC CAACCCAAGT GAATTCAGAA ATGATGACGC ACGTCCTCTC CACAGTCAAT
    ATCATCCTTG GCAAGTCTGC CTTGAATACC TGGAAGATGT CACAGCAGCA AAAAACCAAT
    TATAGCTCAC AGCTATTGGA TTCAGTGGAA AGATTTTCCC GAGTTTTACG AGCAGAAGAC
    AGCACCATCT CCCAACCTAA CGTGCAGATG AAGAGCATGG TCATAAAACC TGGCCACCCC
    CAGTCGTACA GGCAGAGTTT TATTTTCTTG GACTCTGACC TCTGGGGCAA CGTGACCATC
    AATGGATGCC AGCTGGAACA CCTGCAGCCA GATTCATTTG TTGTCACTGT GGCTTTCCCA
    ACTCTCAAAA CCATCCTCGA CAGGGATGTT CCGGGACAGA ACTTTGCCAA CAGCTTGGTG
    ATGACCACCA CCGTCAGCCG CAGCATAACC ACGCCATTCA GGATTCTGAT GACTTTCAAA
    AACAACCACC CTTTGGGCGG GATACCGCAG TGTGTCTTCT GGAACTTCGA CCTCGCCAAC
    CACACCGGGG GGTGGGACAG CAGTGGGTGC TATGTGAAAG AGGTCACTGA GGACAGCGTG
    TCCTGCAGCT GTGAACACCT CACCTCCTTC TCCATCCTCA TGTCCCCCGA CTCCCCAGAC
    CCTGATTCTC TCCTGAAAAT CCTGCTGGAT ATCATTTCCT ACATCGGGCT GTGCTTTTCC
    ATCTTGAGCT TAGCAGCCTG CCTCGTCGTG GAGGCCGTGG TATGGAAATC GGTGACCAAG
    AACCGGACCT CCTCCATGCG CCACATCTGC ATCGTCAACA TCACTGCTTC CCTGCTGGTG
    GCCGACGTCT GGTTCATCGT GGCCGCCGCC ATCCACGACC ATCGTTTCCC GCTCAACGAG
    ACGGCCTGTG TGGCCGCCAC CTTCTTCGTC CACTTCTTCT ACCTCAGTGT CTTCTTCTGG
    ATGCTGACCC TGGGGCTCAT GCTCTTCTAC CGCCTGGTTT TCATTCTACA CGACACGAGC
    AAGTCCATTC AGAAGACGAT CGCCTTTTCT CTGGGGTACG GCTGCCCTCT GGTCATCTCC
    ATCATCACAG TGGGGGCCAC CCAGCCCCGG GAGGTCTACA TGAGGAAGAA CGCCTGCTGG
    CTCAACTGGG AGGACACCAA AGCCCTGCTG GCCTTCGTCA TCCCGGCCCT GATCATCGTG
    GTGGTAAACA TGACCATCAC GGTCGTGGTC ATCACCAAGA TCCTGAGGCC TTCCGTCGGG
    GACAAGCCGA GCAAGCAGGA GAAGAGCAGC CTGTTCCAGG TCGCCAAGAG CATTGGGGTC
    CTCACGCCGC TCTTGGGGCT CACCTGGGGT TTCGGGCTGG CCACCGTGTT CCAGGGGAGC
    AACGCTGTGT TCCACATTGT GTTCACCCTC CTCAATGCCT TCCAGGGATT ATTCATTTTA
    CTCTTTGGAT GCCTCTGGGA TCAGAAGGTG CAGGAAGCCT TGCTGAACAA GTATTCACGG
    TCAAAATGGT CTTCACAGCA CTCAAAGTCA ACGTCCATAG GTTCATCTAC ACCTGTATTT
    TCAATGAGTT CTCCAATATC AAGAAGATTT AACAATTTGT TTGGTAAAAC AGGAACATAC
    AATGTTTCCA CCCCGGAAAC AACCAGTTCA TCCCTGGAAA ACACATCCAG TGCTTATTCA
    TTGCTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32021
    Clone ID Related Accession (Same CDS sequence) XM_005223453.2 , XM_005223454.3 , XM_005223453.3 , XM_005223454.4
    Accession Version XM_005223453.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005223453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGGAG ATAATTCCAG CCACAAAGGG
    GAAGTGCGCG AATCCCAAAG GCTTGTGATG GAAATGAGTA CAGTTCAGAG CACCACCAAG
    AACAGCCTAG AGACAAGTAA AGCCCGGGAG CAGCAAGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGGAACA
    TACAATGTTT CCACCCCGGA AACAACCAGT TCATCCCTGG AAAACACATC CAGTGCTTAT
    TCATTGCTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223510.1
    CDS440..4594
    Translation

    Target ORF information:

    RefSeq Version XM_005223453.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223453.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGGAG ATAATTCCAG CCACAAAGGG
    GAAGTGCGCG AATCCCAAAG GCTTGTGATG GAAATGAGTA CAGTTCAGAG CACCACCAAG
    AACAGCCTAG AGACAAGTAA AGCCCGGGAG CAGCAAGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGGAACA
    TACAATGTTT CCACCCCGGA AACAACCAGT TCATCCCTGG AAAACACATC CAGTGCTTAT
    TCATTGCTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32021
    Clone ID Related Accession (Same CDS sequence) XM_005223453.2 , XM_005223454.3 , XM_005223453.3 , XM_005223454.4
    Accession Version XM_005223454.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223454.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGGAG ATAATTCCAG CCACAAAGGG
    GAAGTGCGCG AATCCCAAAG GCTTGTGATG GAAATGAGTA CAGTTCAGAG CACCACCAAG
    AACAGCCTAG AGACAAGTAA AGCCCGGGAG CAGCAAGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGGAACA
    TACAATGTTT CCACCCCGGA AACAACCAGT TCATCCCTGG AAAACACATC CAGTGCTTAT
    TCATTGCTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223511.1
    CDS508..4662
    Translation

    Target ORF information:

    RefSeq Version XM_005223454.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223454.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGGAG ATAATTCCAG CCACAAAGGG
    GAAGTGCGCG AATCCCAAAG GCTTGTGATG GAAATGAGTA CAGTTCAGAG CACCACCAAG
    AACAGCCTAG AGACAAGTAA AGCCCGGGAG CAGCAAGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGGAACA
    TACAATGTTT CCACCCCGGA AACAACCAGT TCATCCCTGG AAAACACATC CAGTGCTTAT
    TCATTGCTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32021
    Clone ID Related Accession (Same CDS sequence) XM_005223453.2 , XM_005223454.3 , XM_005223453.3 , XM_005223454.4
    Accession Version XM_005223453.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223453.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGGAG ATAATTCCAG CCACAAAGGG
    GAAGTGCGCG AATCCCAAAG GCTTGTGATG GAAATGAGTA CAGTTCAGAG CACCACCAAG
    AACAGCCTAG AGACAAGTAA AGCCCGGGAG CAGCAAGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGGAACA
    TACAATGTTT CCACCCCGGA AACAACCAGT TCATCCCTGG AAAACACATC CAGTGCTTAT
    TCATTGCTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223510.1
    CDS439..4593
    Translation

    Target ORF information:

    RefSeq Version XM_005223453.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223453.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGGAG ATAATTCCAG CCACAAAGGG
    GAAGTGCGCG AATCCCAAAG GCTTGTGATG GAAATGAGTA CAGTTCAGAG CACCACCAAG
    AACAGCCTAG AGACAAGTAA AGCCCGGGAG CAGCAAGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGGAACA
    TACAATGTTT CCACCCCGGA AACAACCAGT TCATCCCTGG AAAACACATC CAGTGCTTAT
    TCATTGCTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32021
    Clone ID Related Accession (Same CDS sequence) XM_005223453.2 , XM_005223454.3 , XM_005223453.3 , XM_005223454.4
    Accession Version XM_005223454.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223454.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGGAG ATAATTCCAG CCACAAAGGG
    GAAGTGCGCG AATCCCAAAG GCTTGTGATG GAAATGAGTA CAGTTCAGAG CACCACCAAG
    AACAGCCTAG AGACAAGTAA AGCCCGGGAG CAGCAAGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGGAACA
    TACAATGTTT CCACCCCGGA AACAACCAGT TCATCCCTGG AAAACACATC CAGTGCTTAT
    TCATTGCTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223511.1
    CDS527..4681
    Translation

    Target ORF information:

    RefSeq Version XM_005223454.4
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223454.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGGAG ATAATTCCAG CCACAAAGGG
    GAAGTGCGCG AATCCCAAAG GCTTGTGATG GAAATGAGTA CAGTTCAGAG CACCACCAAG
    AACAGCCTAG AGACAAGTAA AGCCCGGGAG CAGCAAGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGGAACA
    TACAATGTTT CCACCCCGGA AACAACCAGT TCATCCCTGG AAAACACATC CAGTGCTTAT
    TCATTGCTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32022
    Clone ID Related Accession (Same CDS sequence) XM_005223455.3 , XM_005223455.2 , XM_024983637.1
    Accession Version XM_005223455.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor F5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223455.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGATATT ATCCTGAGAA TGTCGGTCAG ACTCAATGTG
    GGCTTTCAAG AAGACCTCAA GAACTCTTCC TCTGCCCTCT ATAAGTCCTA CAAGACTGAC
    CTGGAAACAG CGTTCTGGAA GGGTTACAGT ATTTTACCAG GCTTCAAATT GGTGACGGTG
    ACAGGGTTCA GGCCTGGAAG TGTGGTTGTG ACGTACGAAA TCACAACAAC AACAACAGCA
    TCACCTGCGT TAATGCGTAA AGCGAACGAG AAAGTCACAC AGAACCTCAA TCAGACCTAC
    AAGATGGAGA GCAGTTCCTA TCAGGTTACT TACAGCAATG AAACGAAATT CTCCATCACA
    CCAGAAATCA TCTTTGAAGG AGATACAGTA ACCCTGATGT GTGAGAATGA AGTTTTTTCC
    ACCAATGTGT CCTGGACGCA GGGGGAAACG GGCCTCGACA TCCAGAACAG CACTGAAGTC
    TCCATCTACA CCTCTGTGCT CAACAACATG ACCTCCGTGT CGCGCCTTAT TATCCACAAC
    ATCACTCGGG GTTTCATGGG TGAATACATC TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GCGTCCAAAA GAAAAGTGGA AGTTATACCC ATCCAGATAC TGGCAAGCGA AGCCACGGAG
    GTGATGTGTG ACAACAATCC TGTGTCTTTG CACTGCTGCA GTGAGGTGAA CGTGAATTGG
    AGTCAAATTG AATGGAAGCA GGAAGGGAGT ATAAGCGTTC CAGGAAACGC TGAAATAGAC
    ACTGACAGTG GCTGCAGCAG ATACACCCTC GTGGCCAATG GGACTCAGTG TCCGAGCGGG
    TCGTCTGGAA CGACAGTGGT CTACACGTGT GAGTTCATCA GTGCCCACGG AGCCAGAGGC
    AGAAAGGACA TAGAAGTGAC ATTCATGTCT GTGGGAGATA ATTCCAGCCA CAAAGGGGAA
    GTGCGCGAAT CCCAAAGGCT TGTGATGGAA ATGAGTACAG TTCAGAGCAC CACCAAGAAC
    AGCCTAGAGA CAAGTAAAGC CCGGGAGCAG CAAGCCAAGC TAAACATAAC CCCGGACCTA
    ATTTCTGTTT CTGAGGGACA AAGCTTTTTT ATAAAGTGCA TCAGCGATAT GAGTAACTAT
    GATGAGATGT ACTGGAACAC TTCTGCTGGA ACTAAAATAT ACAAAAGGTT TTATACCACG
    AGGAGGTATC CTGACAGAGC AGAGTCGGTG CTGACAGTCA AGACCTCAAC CAGGGAGTGG
    AATGGAACCT ATCACTGTGT ATTTCGATAC AAGAATTTGT ACAGGGTCAT GGCCAAAGAT
    GTCATCGTGT ACCCGCTGCC TCTAGAGTCC AACATCATGG TTGATCCTGT GGAAGCTGCA
    ATTCCATGCC ATAGCTCCCA TCACATCAGA TGCTGCATTG AGGAAGATTC AGACTACAGG
    GTTATTTTTC AAGTGGGTTC CTCGTCCTTT CCTGCTGTAA AAGAAGTTAA TGAAAAGCAA
    GTGTGCTACA AACACCATTT CGTGGCAGAC TCGGTTTCCT GTCCAGAAAA TGTGGATGTG
    TTCTGTCACT TTGCCAATGC CGCTAATAAT TCAGTCCAGA GCCCGTCTAT GAAGCTTAAC
    CTGGTTCCTG GGGAGAGCAT CACTTGCCAG GATCCTATCC TAGGTGTTGG GGAGCCTGGG
    AAAGTCATCC AGAAACTTTG CCGGTTCTCG AACATTACCA GAAGCCGTGA GAGTCCTATT
    GGAGGGATCA TAACTTATAG ATGCGTGGGC TCCCAGTGGC AGATTGAGAG AAATGACTGC
    ATCTCTGCTC CAATAAATGC TCTGCTCCAG CTGGCCAAGG CTTTGGTTAA GAGCCCCACC
    CAGGACGAGA AGCTCCCGTC CTACCTAGGG AATCTTTCCA TTAGCACAAA GCAAGTGAAA
    CTTGAAGTCG ACTCATTTTC TGGGACCCTG GGAGCTATTA TTAACATCCT GGACCTGTTC
    TCAACAGTTC CAACCCAAGT GAATTCAGAA ATGATGACGC ACGTCCTCTC CACAGTCAAT
    ATCATCCTTG GCAAGTCTGC CTTGAATACC TGGAAGATGT CACAGCAGCA AAAAACCAAT
    TATAGCTCAC AGCTATTGGA TTCAGTGGAA AGATTTTCCC GAGTTTTACG AGCAGAAGAC
    AGCACCATCT CCCAACCTAA CGTGCAGATG AAGAGCATGG TCATAAAACC TGGCCACCCC
    CAGTCGTACA GGCAGAGTTT TATTTTCTTG GACTCTGACC TCTGGGGCAA CGTGACCATC
    AATGGATGCC AGCTGGAACA CCTGCAGCCA GATTCATTTG TTGTCACTGT GGCTTTCCCA
    ACTCTCAAAA CCATCCTCGA CAGGGATGTT CCGGGACAGA ACTTTGCCAA CAGCTTGGTG
    ATGACCACCA CCGTCAGCCG CAGCATAACC ACGCCATTCA GGATTCTGAT GACTTTCAAA
    AACAACCACC CTTTGGGCGG GATACCGCAG TGTGTCTTCT GGAACTTCGA CCTCGCCAAC
    CACACCGGGG GGTGGGACAG CAGTGGGTGC TATGTGAAAG AGGTCACTGA GGACAGCGTG
    TCCTGCAGCT GTGAACACCT CACCTCCTTC TCCATCCTCA TGTCCCCCGA CTCCCCAGAC
    CCTGATTCTC TCCTGAAAAT CCTGCTGGAT ATCATTTCCT ACATCGGGCT GTGCTTTTCC
    ATCTTGAGCT TAGCAGCCTG CCTCGTCGTG GAGGCCGTGG TATGGAAATC GGTGACCAAG
    AACCGGACCT CCTCCATGCG CCACATCTGC ATCGTCAACA TCACTGCTTC CCTGCTGGTG
    GCCGACGTCT GGTTCATCGT GGCCGCCGCC ATCCACGACC ATCGTTTCCC GCTCAACGAG
    ACGGCCTGTG TGGCCGCCAC CTTCTTCGTC CACTTCTTCT ACCTCAGTGT CTTCTTCTGG
    ATGCTGACCC TGGGGCTCAT GCTCTTCTAC CGCCTGGTTT TCATTCTACA CGACACGAGC
    AAGTCCATTC AGAAGACGAT CGCCTTTTCT CTGGGGTACG GCTGCCCTCT GGTCATCTCC
    ATCATCACAG TGGGGGCCAC CCAGCCCCGG GAGGTCTACA TGAGGAAGAA CGCCTGCTGG
    CTCAACTGGG AGGACACCAA AGCCCTGCTG GCCTTCGTCA TCCCGGCCCT GATCATCGTG
    GTGGTAAACA TGACCATCAC GGTCGTGGTC ATCACCAAGA TCCTGAGGCC TTCCGTCGGG
    GACAAGCCGA GCAAGCAGGA GAAGAGCAGC CTGTTCCAGG TCGCCAAGAG CATTGGGGTC
    CTCACGCCGC TCTTGGGGCT CACCTGGGGT TTCGGGCTGG CCACCGTGTT CCAGGGGAGC
    AACGCTGTGT TCCACATTGT GTTCACCCTC CTCAATGCCT TCCAGGGATT ATTCATTTTA
    CTCTTTGGAT GCCTCTGGGA TCAGAAGGTG CAGGAAGCCT TGCTGAACAA GTATTCACGG
    TCAAAATGGT CTTCACAGCA CTCAAAGTCA ACGTCCATAG GTTCATCTAC ACCTGTATTT
    TCAATGAGTT CTCCAATATC AAGAAGATTT AACAATTTGT TTGGTAAAAC AGGAACATAC
    AATGTTTCCA CCCCGGAAAC AACCAGTTCA TCCCTGGAAA ACACATCCAG TGCTTATTCA
    TTGCTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223512.1
    CDS309..4460
    Translation

    Target ORF information:

    RefSeq Version XM_005223455.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223455.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGATATT ATCCTGAGAA TGTCGGTCAG ACTCAATGTG
    GGCTTTCAAG AAGACCTCAA GAACTCTTCC TCTGCCCTCT ATAAGTCCTA CAAGACTGAC
    CTGGAAACAG CGTTCTGGAA GGGTTACAGT ATTTTACCAG GCTTCAAATT GGTGACGGTG
    ACAGGGTTCA GGCCTGGAAG TGTGGTTGTG ACGTACGAAA TCACAACAAC AACAACAGCA
    TCACCTGCGT TAATGCGTAA AGCGAACGAG AAAGTCACAC AGAACCTCAA TCAGACCTAC
    AAGATGGAGA GCAGTTCCTA TCAGGTTACT TACAGCAATG AAACGAAATT CTCCATCACA
    CCAGAAATCA TCTTTGAAGG AGATACAGTA ACCCTGATGT GTGAGAATGA AGTTTTTTCC
    ACCAATGTGT CCTGGACGCA GGGGGAAACG GGCCTCGACA TCCAGAACAG CACTGAAGTC
    TCCATCTACA CCTCTGTGCT CAACAACATG ACCTCCGTGT CGCGCCTTAT TATCCACAAC
    ATCACTCGGG GTTTCATGGG TGAATACATC TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GCGTCCAAAA GAAAAGTGGA AGTTATACCC ATCCAGATAC TGGCAAGCGA AGCCACGGAG
    GTGATGTGTG ACAACAATCC TGTGTCTTTG CACTGCTGCA GTGAGGTGAA CGTGAATTGG
    AGTCAAATTG AATGGAAGCA GGAAGGGAGT ATAAGCGTTC CAGGAAACGC TGAAATAGAC
    ACTGACAGTG GCTGCAGCAG ATACACCCTC GTGGCCAATG GGACTCAGTG TCCGAGCGGG
    TCGTCTGGAA CGACAGTGGT CTACACGTGT GAGTTCATCA GTGCCCACGG AGCCAGAGGC
    AGAAAGGACA TAGAAGTGAC ATTCATGTCT GTGGGAGATA ATTCCAGCCA CAAAGGGGAA
    GTGCGCGAAT CCCAAAGGCT TGTGATGGAA ATGAGTACAG TTCAGAGCAC CACCAAGAAC
    AGCCTAGAGA CAAGTAAAGC CCGGGAGCAG CAAGCCAAGC TAAACATAAC CCCGGACCTA
    ATTTCTGTTT CTGAGGGACA AAGCTTTTTT ATAAAGTGCA TCAGCGATAT GAGTAACTAT
    GATGAGATGT ACTGGAACAC TTCTGCTGGA ACTAAAATAT ACAAAAGGTT TTATACCACG
    AGGAGGTATC CTGACAGAGC AGAGTCGGTG CTGACAGTCA AGACCTCAAC CAGGGAGTGG
    AATGGAACCT ATCACTGTGT ATTTCGATAC AAGAATTTGT ACAGGGTCAT GGCCAAAGAT
    GTCATCGTGT ACCCGCTGCC TCTAGAGTCC AACATCATGG TTGATCCTGT GGAAGCTGCA
    ATTCCATGCC ATAGCTCCCA TCACATCAGA TGCTGCATTG AGGAAGATTC AGACTACAGG
    GTTATTTTTC AAGTGGGTTC CTCGTCCTTT CCTGCTGTAA AAGAAGTTAA TGAAAAGCAA
    GTGTGCTACA AACACCATTT CGTGGCAGAC TCGGTTTCCT GTCCAGAAAA TGTGGATGTG
    TTCTGTCACT TTGCCAATGC CGCTAATAAT TCAGTCCAGA GCCCGTCTAT GAAGCTTAAC
    CTGGTTCCTG GGGAGAGCAT CACTTGCCAG GATCCTATCC TAGGTGTTGG GGAGCCTGGG
    AAAGTCATCC AGAAACTTTG CCGGTTCTCG AACATTACCA GAAGCCGTGA GAGTCCTATT
    GGAGGGATCA TAACTTATAG ATGCGTGGGC TCCCAGTGGC AGATTGAGAG AAATGACTGC
    ATCTCTGCTC CAATAAATGC TCTGCTCCAG CTGGCCAAGG CTTTGGTTAA GAGCCCCACC
    CAGGACGAGA AGCTCCCGTC CTACCTAGGG AATCTTTCCA TTAGCACAAA GCAAGTGAAA
    CTTGAAGTCG ACTCATTTTC TGGGACCCTG GGAGCTATTA TTAACATCCT GGACCTGTTC
    TCAACAGTTC CAACCCAAGT GAATTCAGAA ATGATGACGC ACGTCCTCTC CACAGTCAAT
    ATCATCCTTG GCAAGTCTGC CTTGAATACC TGGAAGATGT CACAGCAGCA AAAAACCAAT
    TATAGCTCAC AGCTATTGGA TTCAGTGGAA AGATTTTCCC GAGTTTTACG AGCAGAAGAC
    AGCACCATCT CCCAACCTAA CGTGCAGATG AAGAGCATGG TCATAAAACC TGGCCACCCC
    CAGTCGTACA GGCAGAGTTT TATTTTCTTG GACTCTGACC TCTGGGGCAA CGTGACCATC
    AATGGATGCC AGCTGGAACA CCTGCAGCCA GATTCATTTG TTGTCACTGT GGCTTTCCCA
    ACTCTCAAAA CCATCCTCGA CAGGGATGTT CCGGGACAGA ACTTTGCCAA CAGCTTGGTG
    ATGACCACCA CCGTCAGCCG CAGCATAACC ACGCCATTCA GGATTCTGAT GACTTTCAAA
    AACAACCACC CTTTGGGCGG GATACCGCAG TGTGTCTTCT GGAACTTCGA CCTCGCCAAC
    CACACCGGGG GGTGGGACAG CAGTGGGTGC TATGTGAAAG AGGTCACTGA GGACAGCGTG
    TCCTGCAGCT GTGAACACCT CACCTCCTTC TCCATCCTCA TGTCCCCCGA CTCCCCAGAC
    CCTGATTCTC TCCTGAAAAT CCTGCTGGAT ATCATTTCCT ACATCGGGCT GTGCTTTTCC
    ATCTTGAGCT TAGCAGCCTG CCTCGTCGTG GAGGCCGTGG TATGGAAATC GGTGACCAAG
    AACCGGACCT CCTCCATGCG CCACATCTGC ATCGTCAACA TCACTGCTTC CCTGCTGGTG
    GCCGACGTCT GGTTCATCGT GGCCGCCGCC ATCCACGACC ATCGTTTCCC GCTCAACGAG
    ACGGCCTGTG TGGCCGCCAC CTTCTTCGTC CACTTCTTCT ACCTCAGTGT CTTCTTCTGG
    ATGCTGACCC TGGGGCTCAT GCTCTTCTAC CGCCTGGTTT TCATTCTACA CGACACGAGC
    AAGTCCATTC AGAAGACGAT CGCCTTTTCT CTGGGGTACG GCTGCCCTCT GGTCATCTCC
    ATCATCACAG TGGGGGCCAC CCAGCCCCGG GAGGTCTACA TGAGGAAGAA CGCCTGCTGG
    CTCAACTGGG AGGACACCAA AGCCCTGCTG GCCTTCGTCA TCCCGGCCCT GATCATCGTG
    GTGGTAAACA TGACCATCAC GGTCGTGGTC ATCACCAAGA TCCTGAGGCC TTCCGTCGGG
    GACAAGCCGA GCAAGCAGGA GAAGAGCAGC CTGTTCCAGG TCGCCAAGAG CATTGGGGTC
    CTCACGCCGC TCTTGGGGCT CACCTGGGGT TTCGGGCTGG CCACCGTGTT CCAGGGGAGC
    AACGCTGTGT TCCACATTGT GTTCACCCTC CTCAATGCCT TCCAGGGATT ATTCATTTTA
    CTCTTTGGAT GCCTCTGGGA TCAGAAGGTG CAGGAAGCCT TGCTGAACAA GTATTCACGG
    TCAAAATGGT CTTCACAGCA CTCAAAGTCA ACGTCCATAG GTTCATCTAC ACCTGTATTT
    TCAATGAGTT CTCCAATATC AAGAAGATTT AACAATTTGT TTGGTAAAAC AGGAACATAC
    AATGTTTCCA CCCCGGAAAC AACCAGTTCA TCCCTGGAAA ACACATCCAG TGCTTATTCA
    TTGCTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32022
    Clone ID Related Accession (Same CDS sequence) XM_005223455.3 , XM_005223455.2 , XM_024983637.1
    Accession Version XM_005223455.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor F5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005223455.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGATATT ATCCTGAGAA TGTCGGTCAG ACTCAATGTG
    GGCTTTCAAG AAGACCTCAA GAACTCTTCC TCTGCCCTCT ATAAGTCCTA CAAGACTGAC
    CTGGAAACAG CGTTCTGGAA GGGTTACAGT ATTTTACCAG GCTTCAAATT GGTGACGGTG
    ACAGGGTTCA GGCCTGGAAG TGTGGTTGTG ACGTACGAAA TCACAACAAC AACAACAGCA
    TCACCTGCGT TAATGCGTAA AGCGAACGAG AAAGTCACAC AGAACCTCAA TCAGACCTAC
    AAGATGGAGA GCAGTTCCTA TCAGGTTACT TACAGCAATG AAACGAAATT CTCCATCACA
    CCAGAAATCA TCTTTGAAGG AGATACAGTA ACCCTGATGT GTGAGAATGA AGTTTTTTCC
    ACCAATGTGT CCTGGACGCA GGGGGAAACG GGCCTCGACA TCCAGAACAG CACTGAAGTC
    TCCATCTACA CCTCTGTGCT CAACAACATG ACCTCCGTGT CGCGCCTTAT TATCCACAAC
    ATCACTCGGG GTTTCATGGG TGAATACATC TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GCGTCCAAAA GAAAAGTGGA AGTTATACCC ATCCAGATAC TGGCAAGCGA AGCCACGGAG
    GTGATGTGTG ACAACAATCC TGTGTCTTTG CACTGCTGCA GTGAGGTGAA CGTGAATTGG
    AGTCAAATTG AATGGAAGCA GGAAGGGAGT ATAAGCGTTC CAGGAAACGC TGAAATAGAC
    ACTGACAGTG GCTGCAGCAG ATACACCCTC GTGGCCAATG GGACTCAGTG TCCGAGCGGG
    TCGTCTGGAA CGACAGTGGT CTACACGTGT GAGTTCATCA GTGCCCACGG AGCCAGAGGC
    AGAAAGGACA TAGAAGTGAC ATTCATGTCT GTGGGAGATA ATTCCAGCCA CAAAGGGGAA
    GTGCGCGAAT CCCAAAGGCT TGTGATGGAA ATGAGTACAG TTCAGAGCAC CACCAAGAAC
    AGCCTAGAGA CAAGTAAAGC CCGGGAGCAG CAAGCCAAGC TAAACATAAC CCCGGACCTA
    ATTTCTGTTT CTGAGGGACA AAGCTTTTTT ATAAAGTGCA TCAGCGATAT GAGTAACTAT
    GATGAGATGT ACTGGAACAC TTCTGCTGGA ACTAAAATAT ACAAAAGGTT TTATACCACG
    AGGAGGTATC CTGACAGAGC AGAGTCGGTG CTGACAGTCA AGACCTCAAC CAGGGAGTGG
    AATGGAACCT ATCACTGTGT ATTTCGATAC AAGAATTTGT ACAGGGTCAT GGCCAAAGAT
    GTCATCGTGT ACCCGCTGCC TCTAGAGTCC AACATCATGG TTGATCCTGT GGAAGCTGCA
    ATTCCATGCC ATAGCTCCCA TCACATCAGA TGCTGCATTG AGGAAGATTC AGACTACAGG
    GTTATTTTTC AAGTGGGTTC CTCGTCCTTT CCTGCTGTAA AAGAAGTTAA TGAAAAGCAA
    GTGTGCTACA AACACCATTT CGTGGCAGAC TCGGTTTCCT GTCCAGAAAA TGTGGATGTG
    TTCTGTCACT TTGCCAATGC CGCTAATAAT TCAGTCCAGA GCCCGTCTAT GAAGCTTAAC
    CTGGTTCCTG GGGAGAGCAT CACTTGCCAG GATCCTATCC TAGGTGTTGG GGAGCCTGGG
    AAAGTCATCC AGAAACTTTG CCGGTTCTCG AACATTACCA GAAGCCGTGA GAGTCCTATT
    GGAGGGATCA TAACTTATAG ATGCGTGGGC TCCCAGTGGC AGATTGAGAG AAATGACTGC
    ATCTCTGCTC CAATAAATGC TCTGCTCCAG CTGGCCAAGG CTTTGGTTAA GAGCCCCACC
    CAGGACGAGA AGCTCCCGTC CTACCTAGGG AATCTTTCCA TTAGCACAAA GCAAGTGAAA
    CTTGAAGTCG ACTCATTTTC TGGGACCCTG GGAGCTATTA TTAACATCCT GGACCTGTTC
    TCAACAGTTC CAACCCAAGT GAATTCAGAA ATGATGACGC ACGTCCTCTC CACAGTCAAT
    ATCATCCTTG GCAAGTCTGC CTTGAATACC TGGAAGATGT CACAGCAGCA AAAAACCAAT
    TATAGCTCAC AGCTATTGGA TTCAGTGGAA AGATTTTCCC GAGTTTTACG AGCAGAAGAC
    AGCACCATCT CCCAACCTAA CGTGCAGATG AAGAGCATGG TCATAAAACC TGGCCACCCC
    CAGTCGTACA GGCAGAGTTT TATTTTCTTG GACTCTGACC TCTGGGGCAA CGTGACCATC
    AATGGATGCC AGCTGGAACA CCTGCAGCCA GATTCATTTG TTGTCACTGT GGCTTTCCCA
    ACTCTCAAAA CCATCCTCGA CAGGGATGTT CCGGGACAGA ACTTTGCCAA CAGCTTGGTG
    ATGACCACCA CCGTCAGCCG CAGCATAACC ACGCCATTCA GGATTCTGAT GACTTTCAAA
    AACAACCACC CTTTGGGCGG GATACCGCAG TGTGTCTTCT GGAACTTCGA CCTCGCCAAC
    CACACCGGGG GGTGGGACAG CAGTGGGTGC TATGTGAAAG AGGTCACTGA GGACAGCGTG
    TCCTGCAGCT GTGAACACCT CACCTCCTTC TCCATCCTCA TGTCCCCCGA CTCCCCAGAC
    CCTGATTCTC TCCTGAAAAT CCTGCTGGAT ATCATTTCCT ACATCGGGCT GTGCTTTTCC
    ATCTTGAGCT TAGCAGCCTG CCTCGTCGTG GAGGCCGTGG TATGGAAATC GGTGACCAAG
    AACCGGACCT CCTCCATGCG CCACATCTGC ATCGTCAACA TCACTGCTTC CCTGCTGGTG
    GCCGACGTCT GGTTCATCGT GGCCGCCGCC ATCCACGACC ATCGTTTCCC GCTCAACGAG
    ACGGCCTGTG TGGCCGCCAC CTTCTTCGTC CACTTCTTCT ACCTCAGTGT CTTCTTCTGG
    ATGCTGACCC TGGGGCTCAT GCTCTTCTAC CGCCTGGTTT TCATTCTACA CGACACGAGC
    AAGTCCATTC AGAAGACGAT CGCCTTTTCT CTGGGGTACG GCTGCCCTCT GGTCATCTCC
    ATCATCACAG TGGGGGCCAC CCAGCCCCGG GAGGTCTACA TGAGGAAGAA CGCCTGCTGG
    CTCAACTGGG AGGACACCAA AGCCCTGCTG GCCTTCGTCA TCCCGGCCCT GATCATCGTG
    GTGGTAAACA TGACCATCAC GGTCGTGGTC ATCACCAAGA TCCTGAGGCC TTCCGTCGGG
    GACAAGCCGA GCAAGCAGGA GAAGAGCAGC CTGTTCCAGG TCGCCAAGAG CATTGGGGTC
    CTCACGCCGC TCTTGGGGCT CACCTGGGGT TTCGGGCTGG CCACCGTGTT CCAGGGGAGC
    AACGCTGTGT TCCACATTGT GTTCACCCTC CTCAATGCCT TCCAGGGATT ATTCATTTTA
    CTCTTTGGAT GCCTCTGGGA TCAGAAGGTG CAGGAAGCCT TGCTGAACAA GTATTCACGG
    TCAAAATGGT CTTCACAGCA CTCAAAGTCA ACGTCCATAG GTTCATCTAC ACCTGTATTT
    TCAATGAGTT CTCCAATATC AAGAAGATTT AACAATTTGT TTGGTAAAAC AGGAACATAC
    AATGTTTCCA CCCCGGAAAC AACCAGTTCA TCCCTGGAAA ACACATCCAG TGCTTATTCA
    TTGCTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223512.1
    CDS440..4591
    Translation

    Target ORF information:

    RefSeq Version XM_005223455.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223455.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGATATT ATCCTGAGAA TGTCGGTCAG ACTCAATGTG
    GGCTTTCAAG AAGACCTCAA GAACTCTTCC TCTGCCCTCT ATAAGTCCTA CAAGACTGAC
    CTGGAAACAG CGTTCTGGAA GGGTTACAGT ATTTTACCAG GCTTCAAATT GGTGACGGTG
    ACAGGGTTCA GGCCTGGAAG TGTGGTTGTG ACGTACGAAA TCACAACAAC AACAACAGCA
    TCACCTGCGT TAATGCGTAA AGCGAACGAG AAAGTCACAC AGAACCTCAA TCAGACCTAC
    AAGATGGAGA GCAGTTCCTA TCAGGTTACT TACAGCAATG AAACGAAATT CTCCATCACA
    CCAGAAATCA TCTTTGAAGG AGATACAGTA ACCCTGATGT GTGAGAATGA AGTTTTTTCC
    ACCAATGTGT CCTGGACGCA GGGGGAAACG GGCCTCGACA TCCAGAACAG CACTGAAGTC
    TCCATCTACA CCTCTGTGCT CAACAACATG ACCTCCGTGT CGCGCCTTAT TATCCACAAC
    ATCACTCGGG GTTTCATGGG TGAATACATC TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GCGTCCAAAA GAAAAGTGGA AGTTATACCC ATCCAGATAC TGGCAAGCGA AGCCACGGAG
    GTGATGTGTG ACAACAATCC TGTGTCTTTG CACTGCTGCA GTGAGGTGAA CGTGAATTGG
    AGTCAAATTG AATGGAAGCA GGAAGGGAGT ATAAGCGTTC CAGGAAACGC TGAAATAGAC
    ACTGACAGTG GCTGCAGCAG ATACACCCTC GTGGCCAATG GGACTCAGTG TCCGAGCGGG
    TCGTCTGGAA CGACAGTGGT CTACACGTGT GAGTTCATCA GTGCCCACGG AGCCAGAGGC
    AGAAAGGACA TAGAAGTGAC ATTCATGTCT GTGGGAGATA ATTCCAGCCA CAAAGGGGAA
    GTGCGCGAAT CCCAAAGGCT TGTGATGGAA ATGAGTACAG TTCAGAGCAC CACCAAGAAC
    AGCCTAGAGA CAAGTAAAGC CCGGGAGCAG CAAGCCAAGC TAAACATAAC CCCGGACCTA
    ATTTCTGTTT CTGAGGGACA AAGCTTTTTT ATAAAGTGCA TCAGCGATAT GAGTAACTAT
    GATGAGATGT ACTGGAACAC TTCTGCTGGA ACTAAAATAT ACAAAAGGTT TTATACCACG
    AGGAGGTATC CTGACAGAGC AGAGTCGGTG CTGACAGTCA AGACCTCAAC CAGGGAGTGG
    AATGGAACCT ATCACTGTGT ATTTCGATAC AAGAATTTGT ACAGGGTCAT GGCCAAAGAT
    GTCATCGTGT ACCCGCTGCC TCTAGAGTCC AACATCATGG TTGATCCTGT GGAAGCTGCA
    ATTCCATGCC ATAGCTCCCA TCACATCAGA TGCTGCATTG AGGAAGATTC AGACTACAGG
    GTTATTTTTC AAGTGGGTTC CTCGTCCTTT CCTGCTGTAA AAGAAGTTAA TGAAAAGCAA
    GTGTGCTACA AACACCATTT CGTGGCAGAC TCGGTTTCCT GTCCAGAAAA TGTGGATGTG
    TTCTGTCACT TTGCCAATGC CGCTAATAAT TCAGTCCAGA GCCCGTCTAT GAAGCTTAAC
    CTGGTTCCTG GGGAGAGCAT CACTTGCCAG GATCCTATCC TAGGTGTTGG GGAGCCTGGG
    AAAGTCATCC AGAAACTTTG CCGGTTCTCG AACATTACCA GAAGCCGTGA GAGTCCTATT
    GGAGGGATCA TAACTTATAG ATGCGTGGGC TCCCAGTGGC AGATTGAGAG AAATGACTGC
    ATCTCTGCTC CAATAAATGC TCTGCTCCAG CTGGCCAAGG CTTTGGTTAA GAGCCCCACC
    CAGGACGAGA AGCTCCCGTC CTACCTAGGG AATCTTTCCA TTAGCACAAA GCAAGTGAAA
    CTTGAAGTCG ACTCATTTTC TGGGACCCTG GGAGCTATTA TTAACATCCT GGACCTGTTC
    TCAACAGTTC CAACCCAAGT GAATTCAGAA ATGATGACGC ACGTCCTCTC CACAGTCAAT
    ATCATCCTTG GCAAGTCTGC CTTGAATACC TGGAAGATGT CACAGCAGCA AAAAACCAAT
    TATAGCTCAC AGCTATTGGA TTCAGTGGAA AGATTTTCCC GAGTTTTACG AGCAGAAGAC
    AGCACCATCT CCCAACCTAA CGTGCAGATG AAGAGCATGG TCATAAAACC TGGCCACCCC
    CAGTCGTACA GGCAGAGTTT TATTTTCTTG GACTCTGACC TCTGGGGCAA CGTGACCATC
    AATGGATGCC AGCTGGAACA CCTGCAGCCA GATTCATTTG TTGTCACTGT GGCTTTCCCA
    ACTCTCAAAA CCATCCTCGA CAGGGATGTT CCGGGACAGA ACTTTGCCAA CAGCTTGGTG
    ATGACCACCA CCGTCAGCCG CAGCATAACC ACGCCATTCA GGATTCTGAT GACTTTCAAA
    AACAACCACC CTTTGGGCGG GATACCGCAG TGTGTCTTCT GGAACTTCGA CCTCGCCAAC
    CACACCGGGG GGTGGGACAG CAGTGGGTGC TATGTGAAAG AGGTCACTGA GGACAGCGTG
    TCCTGCAGCT GTGAACACCT CACCTCCTTC TCCATCCTCA TGTCCCCCGA CTCCCCAGAC
    CCTGATTCTC TCCTGAAAAT CCTGCTGGAT ATCATTTCCT ACATCGGGCT GTGCTTTTCC
    ATCTTGAGCT TAGCAGCCTG CCTCGTCGTG GAGGCCGTGG TATGGAAATC GGTGACCAAG
    AACCGGACCT CCTCCATGCG CCACATCTGC ATCGTCAACA TCACTGCTTC CCTGCTGGTG
    GCCGACGTCT GGTTCATCGT GGCCGCCGCC ATCCACGACC ATCGTTTCCC GCTCAACGAG
    ACGGCCTGTG TGGCCGCCAC CTTCTTCGTC CACTTCTTCT ACCTCAGTGT CTTCTTCTGG
    ATGCTGACCC TGGGGCTCAT GCTCTTCTAC CGCCTGGTTT TCATTCTACA CGACACGAGC
    AAGTCCATTC AGAAGACGAT CGCCTTTTCT CTGGGGTACG GCTGCCCTCT GGTCATCTCC
    ATCATCACAG TGGGGGCCAC CCAGCCCCGG GAGGTCTACA TGAGGAAGAA CGCCTGCTGG
    CTCAACTGGG AGGACACCAA AGCCCTGCTG GCCTTCGTCA TCCCGGCCCT GATCATCGTG
    GTGGTAAACA TGACCATCAC GGTCGTGGTC ATCACCAAGA TCCTGAGGCC TTCCGTCGGG
    GACAAGCCGA GCAAGCAGGA GAAGAGCAGC CTGTTCCAGG TCGCCAAGAG CATTGGGGTC
    CTCACGCCGC TCTTGGGGCT CACCTGGGGT TTCGGGCTGG CCACCGTGTT CCAGGGGAGC
    AACGCTGTGT TCCACATTGT GTTCACCCTC CTCAATGCCT TCCAGGGATT ATTCATTTTA
    CTCTTTGGAT GCCTCTGGGA TCAGAAGGTG CAGGAAGCCT TGCTGAACAA GTATTCACGG
    TCAAAATGGT CTTCACAGCA CTCAAAGTCA ACGTCCATAG GTTCATCTAC ACCTGTATTT
    TCAATGAGTT CTCCAATATC AAGAAGATTT AACAATTTGT TTGGTAAAAC AGGAACATAC
    AATGTTTCCA CCCCGGAAAC AACCAGTTCA TCCCTGGAAA ACACATCCAG TGCTTATTCA
    TTGCTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32022
    Clone ID Related Accession (Same CDS sequence) XM_005223455.3 , XM_005223455.2 , XM_024983637.1
    Accession Version XM_024983637.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor F5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGATATT ATCCTGAGAA TGTCGGTCAG ACTCAATGTG
    GGCTTTCAAG AAGACCTCAA GAACTCTTCC TCTGCCCTCT ATAAGTCCTA CAAGACTGAC
    CTGGAAACAG CGTTCTGGAA GGGTTACAGT ATTTTACCAG GCTTCAAATT GGTGACGGTG
    ACAGGGTTCA GGCCTGGAAG TGTGGTTGTG ACGTACGAAA TCACAACAAC AACAACAGCA
    TCACCTGCGT TAATGCGTAA AGCGAACGAG AAAGTCACAC AGAACCTCAA TCAGACCTAC
    AAGATGGAGA GCAGTTCCTA TCAGGTTACT TACAGCAATG AAACGAAATT CTCCATCACA
    CCAGAAATCA TCTTTGAAGG AGATACAGTA ACCCTGATGT GTGAGAATGA AGTTTTTTCC
    ACCAATGTGT CCTGGACGCA GGGGGAAACG GGCCTCGACA TCCAGAACAG CACTGAAGTC
    TCCATCTACA CCTCTGTGCT CAACAACATG ACCTCCGTGT CGCGCCTTAT TATCCACAAC
    ATCACTCGGG GTTTCATGGG TGAATACATC TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GCGTCCAAAA GAAAAGTGGA AGTTATACCC ATCCAGATAC TGGCAAGCGA AGCCACGGAG
    GTGATGTGTG ACAACAATCC TGTGTCTTTG CACTGCTGCA GTGAGGTGAA CGTGAATTGG
    AGTCAAATTG AATGGAAGCA GGAAGGGAGT ATAAGCGTTC CAGGAAACGC TGAAATAGAC
    ACTGACAGTG GCTGCAGCAG ATACACCCTC GTGGCCAATG GGACTCAGTG TCCGAGCGGG
    TCGTCTGGAA CGACAGTGGT CTACACGTGT GAGTTCATCA GTGCCCACGG AGCCAGAGGC
    AGAAAGGACA TAGAAGTGAC ATTCATGTCT GTGGGAGATA ATTCCAGCCA CAAAGGGGAA
    GTGCGCGAAT CCCAAAGGCT TGTGATGGAA ATGAGTACAG TTCAGAGCAC CACCAAGAAC
    AGCCTAGAGA CAAGTAAAGC CCGGGAGCAG CAAGCCAAGC TAAACATAAC CCCGGACCTA
    ATTTCTGTTT CTGAGGGACA AAGCTTTTTT ATAAAGTGCA TCAGCGATAT GAGTAACTAT
    GATGAGATGT ACTGGAACAC TTCTGCTGGA ACTAAAATAT ACAAAAGGTT TTATACCACG
    AGGAGGTATC CTGACAGAGC AGAGTCGGTG CTGACAGTCA AGACCTCAAC CAGGGAGTGG
    AATGGAACCT ATCACTGTGT ATTTCGATAC AAGAATTTGT ACAGGGTCAT GGCCAAAGAT
    GTCATCGTGT ACCCGCTGCC TCTAGAGTCC AACATCATGG TTGATCCTGT GGAAGCTGCA
    ATTCCATGCC ATAGCTCCCA TCACATCAGA TGCTGCATTG AGGAAGATTC AGACTACAGG
    GTTATTTTTC AAGTGGGTTC CTCGTCCTTT CCTGCTGTAA AAGAAGTTAA TGAAAAGCAA
    GTGTGCTACA AACACCATTT CGTGGCAGAC TCGGTTTCCT GTCCAGAAAA TGTGGATGTG
    TTCTGTCACT TTGCCAATGC CGCTAATAAT TCAGTCCAGA GCCCGTCTAT GAAGCTTAAC
    CTGGTTCCTG GGGAGAGCAT CACTTGCCAG GATCCTATCC TAGGTGTTGG GGAGCCTGGG
    AAAGTCATCC AGAAACTTTG CCGGTTCTCG AACATTACCA GAAGCCGTGA GAGTCCTATT
    GGAGGGATCA TAACTTATAG ATGCGTGGGC TCCCAGTGGC AGATTGAGAG AAATGACTGC
    ATCTCTGCTC CAATAAATGC TCTGCTCCAG CTGGCCAAGG CTTTGGTTAA GAGCCCCACC
    CAGGACGAGA AGCTCCCGTC CTACCTAGGG AATCTTTCCA TTAGCACAAA GCAAGTGAAA
    CTTGAAGTCG ACTCATTTTC TGGGACCCTG GGAGCTATTA TTAACATCCT GGACCTGTTC
    TCAACAGTTC CAACCCAAGT GAATTCAGAA ATGATGACGC ACGTCCTCTC CACAGTCAAT
    ATCATCCTTG GCAAGTCTGC CTTGAATACC TGGAAGATGT CACAGCAGCA AAAAACCAAT
    TATAGCTCAC AGCTATTGGA TTCAGTGGAA AGATTTTCCC GAGTTTTACG AGCAGAAGAC
    AGCACCATCT CCCAACCTAA CGTGCAGATG AAGAGCATGG TCATAAAACC TGGCCACCCC
    CAGTCGTACA GGCAGAGTTT TATTTTCTTG GACTCTGACC TCTGGGGCAA CGTGACCATC
    AATGGATGCC AGCTGGAACA CCTGCAGCCA GATTCATTTG TTGTCACTGT GGCTTTCCCA
    ACTCTCAAAA CCATCCTCGA CAGGGATGTT CCGGGACAGA ACTTTGCCAA CAGCTTGGTG
    ATGACCACCA CCGTCAGCCG CAGCATAACC ACGCCATTCA GGATTCTGAT GACTTTCAAA
    AACAACCACC CTTTGGGCGG GATACCGCAG TGTGTCTTCT GGAACTTCGA CCTCGCCAAC
    CACACCGGGG GGTGGGACAG CAGTGGGTGC TATGTGAAAG AGGTCACTGA GGACAGCGTG
    TCCTGCAGCT GTGAACACCT CACCTCCTTC TCCATCCTCA TGTCCCCCGA CTCCCCAGAC
    CCTGATTCTC TCCTGAAAAT CCTGCTGGAT ATCATTTCCT ACATCGGGCT GTGCTTTTCC
    ATCTTGAGCT TAGCAGCCTG CCTCGTCGTG GAGGCCGTGG TATGGAAATC GGTGACCAAG
    AACCGGACCT CCTCCATGCG CCACATCTGC ATCGTCAACA TCACTGCTTC CCTGCTGGTG
    GCCGACGTCT GGTTCATCGT GGCCGCCGCC ATCCACGACC ATCGTTTCCC GCTCAACGAG
    ACGGCCTGTG TGGCCGCCAC CTTCTTCGTC CACTTCTTCT ACCTCAGTGT CTTCTTCTGG
    ATGCTGACCC TGGGGCTCAT GCTCTTCTAC CGCCTGGTTT TCATTCTACA CGACACGAGC
    AAGTCCATTC AGAAGACGAT CGCCTTTTCT CTGGGGTACG GCTGCCCTCT GGTCATCTCC
    ATCATCACAG TGGGGGCCAC CCAGCCCCGG GAGGTCTACA TGAGGAAGAA CGCCTGCTGG
    CTCAACTGGG AGGACACCAA AGCCCTGCTG GCCTTCGTCA TCCCGGCCCT GATCATCGTG
    GTGGTAAACA TGACCATCAC GGTCGTGGTC ATCACCAAGA TCCTGAGGCC TTCCGTCGGG
    GACAAGCCGA GCAAGCAGGA GAAGAGCAGC CTGTTCCAGG TCGCCAAGAG CATTGGGGTC
    CTCACGCCGC TCTTGGGGCT CACCTGGGGT TTCGGGCTGG CCACCGTGTT CCAGGGGAGC
    AACGCTGTGT TCCACATTGT GTTCACCCTC CTCAATGCCT TCCAGGGATT ATTCATTTTA
    CTCTTTGGAT GCCTCTGGGA TCAGAAGGTG CAGGAAGCCT TGCTGAACAA GTATTCACGG
    TCAAAATGGT CTTCACAGCA CTCAAAGTCA ACGTCCATAG GTTCATCTAC ACCTGTATTT
    TCAATGAGTT CTCCAATATC AAGAAGATTT AACAATTTGT TTGGTAAAAC AGGAACATAC
    AATGTTTCCA CCCCGGAAAC AACCAGTTCA TCCCTGGAAA ACACATCCAG TGCTTATTCA
    TTGCTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839405.1
    CDS236..4387
    Translation

    Target ORF information:

    RefSeq Version XM_024983637.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983637.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGATATT ATCCTGAGAA TGTCGGTCAG ACTCAATGTG
    GGCTTTCAAG AAGACCTCAA GAACTCTTCC TCTGCCCTCT ATAAGTCCTA CAAGACTGAC
    CTGGAAACAG CGTTCTGGAA GGGTTACAGT ATTTTACCAG GCTTCAAATT GGTGACGGTG
    ACAGGGTTCA GGCCTGGAAG TGTGGTTGTG ACGTACGAAA TCACAACAAC AACAACAGCA
    TCACCTGCGT TAATGCGTAA AGCGAACGAG AAAGTCACAC AGAACCTCAA TCAGACCTAC
    AAGATGGAGA GCAGTTCCTA TCAGGTTACT TACAGCAATG AAACGAAATT CTCCATCACA
    CCAGAAATCA TCTTTGAAGG AGATACAGTA ACCCTGATGT GTGAGAATGA AGTTTTTTCC
    ACCAATGTGT CCTGGACGCA GGGGGAAACG GGCCTCGACA TCCAGAACAG CACTGAAGTC
    TCCATCTACA CCTCTGTGCT CAACAACATG ACCTCCGTGT CGCGCCTTAT TATCCACAAC
    ATCACTCGGG GTTTCATGGG TGAATACATC TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GCGTCCAAAA GAAAAGTGGA AGTTATACCC ATCCAGATAC TGGCAAGCGA AGCCACGGAG
    GTGATGTGTG ACAACAATCC TGTGTCTTTG CACTGCTGCA GTGAGGTGAA CGTGAATTGG
    AGTCAAATTG AATGGAAGCA GGAAGGGAGT ATAAGCGTTC CAGGAAACGC TGAAATAGAC
    ACTGACAGTG GCTGCAGCAG ATACACCCTC GTGGCCAATG GGACTCAGTG TCCGAGCGGG
    TCGTCTGGAA CGACAGTGGT CTACACGTGT GAGTTCATCA GTGCCCACGG AGCCAGAGGC
    AGAAAGGACA TAGAAGTGAC ATTCATGTCT GTGGGAGATA ATTCCAGCCA CAAAGGGGAA
    GTGCGCGAAT CCCAAAGGCT TGTGATGGAA ATGAGTACAG TTCAGAGCAC CACCAAGAAC
    AGCCTAGAGA CAAGTAAAGC CCGGGAGCAG CAAGCCAAGC TAAACATAAC CCCGGACCTA
    ATTTCTGTTT CTGAGGGACA AAGCTTTTTT ATAAAGTGCA TCAGCGATAT GAGTAACTAT
    GATGAGATGT ACTGGAACAC TTCTGCTGGA ACTAAAATAT ACAAAAGGTT TTATACCACG
    AGGAGGTATC CTGACAGAGC AGAGTCGGTG CTGACAGTCA AGACCTCAAC CAGGGAGTGG
    AATGGAACCT ATCACTGTGT ATTTCGATAC AAGAATTTGT ACAGGGTCAT GGCCAAAGAT
    GTCATCGTGT ACCCGCTGCC TCTAGAGTCC AACATCATGG TTGATCCTGT GGAAGCTGCA
    ATTCCATGCC ATAGCTCCCA TCACATCAGA TGCTGCATTG AGGAAGATTC AGACTACAGG
    GTTATTTTTC AAGTGGGTTC CTCGTCCTTT CCTGCTGTAA AAGAAGTTAA TGAAAAGCAA
    GTGTGCTACA AACACCATTT CGTGGCAGAC TCGGTTTCCT GTCCAGAAAA TGTGGATGTG
    TTCTGTCACT TTGCCAATGC CGCTAATAAT TCAGTCCAGA GCCCGTCTAT GAAGCTTAAC
    CTGGTTCCTG GGGAGAGCAT CACTTGCCAG GATCCTATCC TAGGTGTTGG GGAGCCTGGG
    AAAGTCATCC AGAAACTTTG CCGGTTCTCG AACATTACCA GAAGCCGTGA GAGTCCTATT
    GGAGGGATCA TAACTTATAG ATGCGTGGGC TCCCAGTGGC AGATTGAGAG AAATGACTGC
    ATCTCTGCTC CAATAAATGC TCTGCTCCAG CTGGCCAAGG CTTTGGTTAA GAGCCCCACC
    CAGGACGAGA AGCTCCCGTC CTACCTAGGG AATCTTTCCA TTAGCACAAA GCAAGTGAAA
    CTTGAAGTCG ACTCATTTTC TGGGACCCTG GGAGCTATTA TTAACATCCT GGACCTGTTC
    TCAACAGTTC CAACCCAAGT GAATTCAGAA ATGATGACGC ACGTCCTCTC CACAGTCAAT
    ATCATCCTTG GCAAGTCTGC CTTGAATACC TGGAAGATGT CACAGCAGCA AAAAACCAAT
    TATAGCTCAC AGCTATTGGA TTCAGTGGAA AGATTTTCCC GAGTTTTACG AGCAGAAGAC
    AGCACCATCT CCCAACCTAA CGTGCAGATG AAGAGCATGG TCATAAAACC TGGCCACCCC
    CAGTCGTACA GGCAGAGTTT TATTTTCTTG GACTCTGACC TCTGGGGCAA CGTGACCATC
    AATGGATGCC AGCTGGAACA CCTGCAGCCA GATTCATTTG TTGTCACTGT GGCTTTCCCA
    ACTCTCAAAA CCATCCTCGA CAGGGATGTT CCGGGACAGA ACTTTGCCAA CAGCTTGGTG
    ATGACCACCA CCGTCAGCCG CAGCATAACC ACGCCATTCA GGATTCTGAT GACTTTCAAA
    AACAACCACC CTTTGGGCGG GATACCGCAG TGTGTCTTCT GGAACTTCGA CCTCGCCAAC
    CACACCGGGG GGTGGGACAG CAGTGGGTGC TATGTGAAAG AGGTCACTGA GGACAGCGTG
    TCCTGCAGCT GTGAACACCT CACCTCCTTC TCCATCCTCA TGTCCCCCGA CTCCCCAGAC
    CCTGATTCTC TCCTGAAAAT CCTGCTGGAT ATCATTTCCT ACATCGGGCT GTGCTTTTCC
    ATCTTGAGCT TAGCAGCCTG CCTCGTCGTG GAGGCCGTGG TATGGAAATC GGTGACCAAG
    AACCGGACCT CCTCCATGCG CCACATCTGC ATCGTCAACA TCACTGCTTC CCTGCTGGTG
    GCCGACGTCT GGTTCATCGT GGCCGCCGCC ATCCACGACC ATCGTTTCCC GCTCAACGAG
    ACGGCCTGTG TGGCCGCCAC CTTCTTCGTC CACTTCTTCT ACCTCAGTGT CTTCTTCTGG
    ATGCTGACCC TGGGGCTCAT GCTCTTCTAC CGCCTGGTTT TCATTCTACA CGACACGAGC
    AAGTCCATTC AGAAGACGAT CGCCTTTTCT CTGGGGTACG GCTGCCCTCT GGTCATCTCC
    ATCATCACAG TGGGGGCCAC CCAGCCCCGG GAGGTCTACA TGAGGAAGAA CGCCTGCTGG
    CTCAACTGGG AGGACACCAA AGCCCTGCTG GCCTTCGTCA TCCCGGCCCT GATCATCGTG
    GTGGTAAACA TGACCATCAC GGTCGTGGTC ATCACCAAGA TCCTGAGGCC TTCCGTCGGG
    GACAAGCCGA GCAAGCAGGA GAAGAGCAGC CTGTTCCAGG TCGCCAAGAG CATTGGGGTC
    CTCACGCCGC TCTTGGGGCT CACCTGGGGT TTCGGGCTGG CCACCGTGTT CCAGGGGAGC
    AACGCTGTGT TCCACATTGT GTTCACCCTC CTCAATGCCT TCCAGGGATT ATTCATTTTA
    CTCTTTGGAT GCCTCTGGGA TCAGAAGGTG CAGGAAGCCT TGCTGAACAA GTATTCACGG
    TCAAAATGGT CTTCACAGCA CTCAAAGTCA ACGTCCATAG GTTCATCTAC ACCTGTATTT
    TCAATGAGTT CTCCAATATC AAGAAGATTT AACAATTTGT TTGGTAAAAC AGGAACATAC
    AATGTTTCCA CCCCGGAAAC AACCAGTTCA TCCCTGGAAA ACACATCCAG TGCTTATTCA
    TTGCTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32023
    Clone ID Related Accession (Same CDS sequence) XM_010818206.1 , XM_010818206.2
    Accession Version XM_010818206.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4140bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor F5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGGAG ATAATTCCAG CCACAAAGGG
    GAAGTGCGCG AATCCCAAAG GCTTGTGATG GAAATGAGTA CAGTTCAGAG CACCACCAAG
    AACAGCCTAG AGACAAGTAA AGCCCGGGAG CAGCAAGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGCAGCC
    CCAAGGGCTC CCCCAGGCAA TGATGGAAAC CCTGTTAGCC GTGGCAGTCC GCCCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816508.1
    CDS440..4579
    Translation

    Target ORF information:

    RefSeq Version XM_010818206.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818206.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGGAG ATAATTCCAG CCACAAAGGG
    GAAGTGCGCG AATCCCAAAG GCTTGTGATG GAAATGAGTA CAGTTCAGAG CACCACCAAG
    AACAGCCTAG AGACAAGTAA AGCCCGGGAG CAGCAAGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGCAGCC
    CCAAGGGCTC CCCCAGGCAA TGATGGAAAC CCTGTTAGCC GTGGCAGTCC GCCCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32023
    Clone ID Related Accession (Same CDS sequence) XM_010818206.1 , XM_010818206.2
    Accession Version XM_010818206.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4140bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor F5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGGAG ATAATTCCAG CCACAAAGGG
    GAAGTGCGCG AATCCCAAAG GCTTGTGATG GAAATGAGTA CAGTTCAGAG CACCACCAAG
    AACAGCCTAG AGACAAGTAA AGCCCGGGAG CAGCAAGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGCAGCC
    CCAAGGGCTC CCCCAGGCAA TGATGGAAAC CCTGTTAGCC GTGGCAGTCC GCCCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816508.1
    CDS439..4578
    Translation

    Target ORF information:

    RefSeq Version XM_010818206.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818206.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGGAG ATAATTCCAG CCACAAAGGG
    GAAGTGCGCG AATCCCAAAG GCTTGTGATG GAAATGAGTA CAGTTCAGAG CACCACCAAG
    AACAGCCTAG AGACAAGTAA AGCCCGGGAG CAGCAAGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGCAGCC
    CCAAGGGCTC CCCCAGGCAA TGATGGAAAC CCTGTTAGCC GTGGCAGTCC GCCCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32024
    Clone ID Related Accession (Same CDS sequence) XM_005223457.3 , XM_005223457.2
    Accession Version XM_005223457.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor F5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223457.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGGAACA
    TACAATGTTT CCACCCCGGA AACAACCAGT TCATCCCTGG AAAACACATC CAGTGCTTAT
    TCATTGCTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223514.1
    CDS266..4300
    Translation

    Target ORF information:

    RefSeq Version XM_005223457.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223457.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGGAACA
    TACAATGTTT CCACCCCGGA AACAACCAGT TCATCCCTGG AAAACACATC CAGTGCTTAT
    TCATTGCTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32024
    Clone ID Related Accession (Same CDS sequence) XM_005223457.3 , XM_005223457.2
    Accession Version XM_005223457.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor F5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005223457.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGGAACA
    TACAATGTTT CCACCCCGGA AACAACCAGT TCATCCCTGG AAAACACATC CAGTGCTTAT
    TCATTGCTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223514.1
    CDS440..4474
    Translation

    Target ORF information:

    RefSeq Version XM_005223457.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223457.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAGTCCC CAAAGAGAAC CACTTTGTGC TTTGTGATTA TTGCCATTTA TTCTTCCCAA 
    GCTACACTGA GTTTGAATTT AGAGTCTATT GTTTATCATT CGCTTCTCCA TGAACATGAA
    CTAATGGAGG AGGATCCAGT AAGACAAAAA CGAGCTGTTG CCATAAGCAG CCCTGCAGCT
    CAAGAGTTCA CCGTCGATGT TGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAACCTATT
    AAAGCTTATT TGAACAACCT CAGTTTTCCA ATTCAAGGGA ATGACACTGA CCTAGCTACT
    GACATTTTGA GCATCGAGGT GTCAACAGTC TGCAGGCCTA CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CAGGCTATGA GTGGCCTCAG GAAAGTTGCC TCCACAATCT CACTTGTCAG
    GGACATGATG GTTCCTCTGC AGGGCATCAC TGCAGTTGCC TTAAGGGACT GCCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT ATTATCCTGA GAATGTCGGT CAGACTCAAT
    GTGGGCTTTC AAGAAGACCT CAAGAACTCT TCCTCTGCCC TCTATAAGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAGGGTTAC AGTATTTTAC CAGGCTTCAA ATTGGTGACG
    GTGACAGGGT TCAGGCCTGG AAGTGTGGTT GTGACGTACG AAATCACAAC AACAACAACA
    GCATCACCTG CGTTAATGCG TAAAGCGAAC GAGAAAGTCA CACAGAACCT CAATCAGACC
    TACAAGATGG AGAGCAGTTC CTATCAGGTT ACTTACAGCA ATGAAACGAA ATTCTCCATC
    ACACCAGAAA TCATCTTTGA AGGAGATACA GTAACCCTGA TGTGTGAGAA TGAAGTTTTT
    TCCACCAATG TGTCCTGGAC GCAGGGGGAA ACGGGCCTCG ACATCCAGAA CAGCACTGAA
    GTCTCCATCT ACACCTCTGT GCTCAACAAC ATGACCTCCG TGTCGCGCCT TATTATCCAC
    AACATCACTC GGGGTTTCAT GGGTGAATAC ATCTGCAAGC TGACATTAGA TATTTTTGAA
    TATGCGTCCA AAAGAAAAGT GGAAGTTATA CCCATCCAGA TACTGGCAAG CGAAGCCACG
    GAGGTGATGT GTGACAACAA TCCTGTGTCT TTGCACTGCT GCAGTGAGGT GAACGTGAAT
    TGGAGTCAAA TTGAATGGAA GCAGGAAGGG AGTATAAGCG TTCCAGGAAA CGCTGAAATA
    GACACTGACA GTGGCTGCAG CAGATACACC CTCGTGGCCA ATGGGACTCA GTGTCCGAGC
    GGGTCGTCTG GAACGACAGT GGTCTACACG TGTGAGTTCA TCAGTGCCCA CGGAGCCAGA
    GGCAGAAAGG ACATAGAAGT GACATTCATG TCTGTGGCCA AGCTAAACAT AACCCCGGAC
    CTAATTTCTG TTTCTGAGGG ACAAAGCTTT TTTATAAAGT GCATCAGCGA TATGAGTAAC
    TATGATGAGA TGTACTGGAA CACTTCTGCT GGAACTAAAA TATACAAAAG GTTTTATACC
    ACGAGGAGGT ATCCTGACAG AGCAGAGTCG GTGCTGACAG TCAAGACCTC AACCAGGGAG
    TGGAATGGAA CCTATCACTG TGTATTTCGA TACAAGAATT TGTACAGGGT CATGGCCAAA
    GATGTCATCG TGTACCCGCT GCCTCTAGAG TCCAACATCA TGGTTGATCC TGTGGAAGCT
    GCAATTCCAT GCCATAGCTC CCATCACATC AGATGCTGCA TTGAGGAAGA TTCAGACTAC
    AGGGTTATTT TTCAAGTGGG TTCCTCGTCC TTTCCTGCTG TAAAAGAAGT TAATGAAAAG
    CAAGTGTGCT ACAAACACCA TTTCGTGGCA GACTCGGTTT CCTGTCCAGA AAATGTGGAT
    GTGTTCTGTC ACTTTGCCAA TGCCGCTAAT AATTCAGTCC AGAGCCCGTC TATGAAGCTT
    AACCTGGTTC CTGGGGAGAG CATCACTTGC CAGGATCCTA TCCTAGGTGT TGGGGAGCCT
    GGGAAAGTCA TCCAGAAACT TTGCCGGTTC TCGAACATTA CCAGAAGCCG TGAGAGTCCT
    ATTGGAGGGA TCATAACTTA TAGATGCGTG GGCTCCCAGT GGCAGATTGA GAGAAATGAC
    TGCATCTCTG CTCCAATAAA TGCTCTGCTC CAGCTGGCCA AGGCTTTGGT TAAGAGCCCC
    ACCCAGGACG AGAAGCTCCC GTCCTACCTA GGGAATCTTT CCATTAGCAC AAAGCAAGTG
    AAACTTGAAG TCGACTCATT TTCTGGGACC CTGGGAGCTA TTATTAACAT CCTGGACCTG
    TTCTCAACAG TTCCAACCCA AGTGAATTCA GAAATGATGA CGCACGTCCT CTCCACAGTC
    AATATCATCC TTGGCAAGTC TGCCTTGAAT ACCTGGAAGA TGTCACAGCA GCAAAAAACC
    AATTATAGCT CACAGCTATT GGATTCAGTG GAAAGATTTT CCCGAGTTTT ACGAGCAGAA
    GACAGCACCA TCTCCCAACC TAACGTGCAG ATGAAGAGCA TGGTCATAAA ACCTGGCCAC
    CCCCAGTCGT ACAGGCAGAG TTTTATTTTC TTGGACTCTG ACCTCTGGGG CAACGTGACC
    ATCAATGGAT GCCAGCTGGA ACACCTGCAG CCAGATTCAT TTGTTGTCAC TGTGGCTTTC
    CCAACTCTCA AAACCATCCT CGACAGGGAT GTTCCGGGAC AGAACTTTGC CAACAGCTTG
    GTGATGACCA CCACCGTCAG CCGCAGCATA ACCACGCCAT TCAGGATTCT GATGACTTTC
    AAAAACAACC ACCCTTTGGG CGGGATACCG CAGTGTGTCT TCTGGAACTT CGACCTCGCC
    AACCACACCG GGGGGTGGGA CAGCAGTGGG TGCTATGTGA AAGAGGTCAC TGAGGACAGC
    GTGTCCTGCA GCTGTGAACA CCTCACCTCC TTCTCCATCC TCATGTCCCC CGACTCCCCA
    GACCCTGATT CTCTCCTGAA AATCCTGCTG GATATCATTT CCTACATCGG GCTGTGCTTT
    TCCATCTTGA GCTTAGCAGC CTGCCTCGTC GTGGAGGCCG TGGTATGGAA ATCGGTGACC
    AAGAACCGGA CCTCCTCCAT GCGCCACATC TGCATCGTCA ACATCACTGC TTCCCTGCTG
    GTGGCCGACG TCTGGTTCAT CGTGGCCGCC GCCATCCACG ACCATCGTTT CCCGCTCAAC
    GAGACGGCCT GTGTGGCCGC CACCTTCTTC GTCCACTTCT TCTACCTCAG TGTCTTCTTC
    TGGATGCTGA CCCTGGGGCT CATGCTCTTC TACCGCCTGG TTTTCATTCT ACACGACACG
    AGCAAGTCCA TTCAGAAGAC GATCGCCTTT TCTCTGGGGT ACGGCTGCCC TCTGGTCATC
    TCCATCATCA CAGTGGGGGC CACCCAGCCC CGGGAGGTCT ACATGAGGAA GAACGCCTGC
    TGGCTCAACT GGGAGGACAC CAAAGCCCTG CTGGCCTTCG TCATCCCGGC CCTGATCATC
    GTGGTGGTAA ACATGACCAT CACGGTCGTG GTCATCACCA AGATCCTGAG GCCTTCCGTC
    GGGGACAAGC CGAGCAAGCA GGAGAAGAGC AGCCTGTTCC AGGTCGCCAA GAGCATTGGG
    GTCCTCACGC CGCTCTTGGG GCTCACCTGG GGTTTCGGGC TGGCCACCGT GTTCCAGGGG
    AGCAACGCTG TGTTCCACAT TGTGTTCACC CTCCTCAATG CCTTCCAGGG ATTATTCATT
    TTACTCTTTG GATGCCTCTG GGATCAGAAG GTGCAGGAAG CCTTGCTGAA CAAGTATTCA
    CGGTCAAAAT GGTCTTCACA GCACTCAAAG TCAACGTCCA TAGGTTCATC TACACCTGTA
    TTTTCAATGA GTTCTCCAAT ATCAAGAAGA TTTAACAATT TGTTTGGTAA AACAGGAACA
    TACAATGTTT CCACCCCGGA AACAACCAGT TCATCCCTGG AAAACACATC CAGTGCTTAT
    TCATTGCTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.