LOC530599 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC530599
Entrez Gene ID	530599
Full Name	laminin subunit beta-2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024983358.1	XP_024839126.1	laminin subunit beta-2 isoform X3
XM_015459648.1	XP_015315134.1	laminin subunit beta-2 isoform X2
XM_015468730.1	XP_015324216.1	laminin subunit beta-2 isoform X1
XM_015459648.2	XP_015315134.2	laminin subunit beta-2 isoform X2
XM_015459647.1	XP_015315133.1	laminin subunit beta-2 isoform X1
XM_015459647.2	XP_015315133.2	laminin subunit beta-2 isoform X1
XM_015468731.1	XP_015324217.1	laminin subunit beta-2 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC530599	XP_002697124.3
Danio rerio (zebrafish)	lamb2l	XP_005162178.1
Xenopus tropicalis (tropical clawed frog)	LOC100170549	NP_001123798.1

Gene Ontology

The following LOC530599 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC530599 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa229390	XM_015459647.2 Latest version!	Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa229391	XM_015459648.2 Latest version!	Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa229392	XM_024983358.1 Latest version!	Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa94461	XM_015459647.1 Latest version!	Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa94461	XM_015468730.1 Latest version!	Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa94462	XM_015468731.1 Latest version!	Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa94462	XM_015459648.1 Latest version!	Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC530599 ( XM_015459647.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315133.2 Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa229390
Clone ID Related Accession (Same CDS sequence)	XM_015459647.2
Accession Version	XM_015459647.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	laminin subunit beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459647.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATG CTGGCTCCCA CTGCCCAGGA GCCCCAGCCG GGCTAGGCCC CCTTCTCTTA 
GCCCTGCTGC TGCTGCTGCC ACCCATGGTC CCGGCTCCCA GAACAAGTGC CCAGGAGGTA 
CCAGACATGA TGCCTCCTCC CGGCTGCTCA GGTGGCAACT GCCACCCACC TACTGGCAAC 
CTGGTTATTG GCCGTGGCCA GAGCCTTCGA ACCTCATCTA CCTGTGGCCT CCATGGCCCT 
GAACTCTACT GTGTTGTCAG TAAACTACAG GCGCTTCTCC AGGACTCTCA GAATTGCTGC 
TTTTGTGATT CTCGGGATGG GAAAAGCCAT GGCATTGAGA ATGTGGTCTC CCGGAGTGAC 
CCTGATGGCA GAAAGACCTG GTGGCAAGCA GAGAGTGGTG TTGAGAATGT CACCATCCAG 
TTGGACCTAG AAGGTGCCTT TTACTTTACC CACCTCATCA TGACTTTTAA GACATTCCGC 
CCAGCAGCTC TGCTCCTTGA GCGCTCAGTG GACCATGGCC ACTCCTGGCA TGTGTTCCGC 
TACTTTGCCC ACAACTGCTC AGGCCTCTTT CCTGGGATCC CTCCTGCCCC TGGCCGCAGA 
GTCAGTGACC TCGTCTGTGA CCAGCGTTAC TCAGACATCG AGCCTGCCAC TGAGGGAGAG 
GTAATTTTCC AAGTTCTGGA CCCATCCTTT CTGGCGGAGA ACACAAACAA CGCGGAGATT 
CAGGAACTCC TGCGTGTGAC TAACCTCCGT GTGAACTTCT CCAAGCTGCA CACACTGGGT 
GACAGGCCCC TGGGGGGCCT CAGGGGCCAC CCCTTCTACT ACTATGCTCT CTATGAGCTA 
GTGATTGCAG GCAGCTGCCT GTGCCACGGC CATGCTTCCG AGTGCAGGCC CGCACCCGGG 
TCCCCACCCA GCGTGGAAGG CATGGTGCAT GGCCATTGTG TCTGCCGCCA CCACACCACT 
GGTACCCACT GTGAACGTTG CCAGGGCCTG TACCAGGACC ACCCGTGGCA GGCAGCAGAG 
CCTGGGTACC CCCACACCTG CCAGGAATGC AAGTGCCATG GGCACGCCCG CAGTTGTCAC 
TTTGACATGG CCCTGTATCT CGCATCTGGC AACGTGAGTG GAGGTGTGTG CGATGCATGT 
CAACACAACA CGGCCGGGCG CCACTGTGAG CTCTGCCAGC CCTTCTTCCA CCGGGACCCC 
CTGGAGGACC CCCGCTCCCT TCACTCCTGC AAACCCTGTG ACTGCAACCC TATGGGTGCC 
CTGGAAGGGG GTTTGTGTGA TGCCTACACA GACACCACCC GGGGTCTCCT CTCTGGGCAG 
TGTCGCTGCA AAGTTCACGT CTGGGGCCAG CGCTGTGACT CCTGCCGGCC AGGTCACTAT 
GGCCTGAGCC TCACCCAGTC AGAAGGCTGC CAGCCCTGCA GGTGCAACGT CCGGGGCCGG 
GTCCCCAGCA CTCAGGCCTG TGATCCATCC AGCGGTGCCT GTCACTGCAA ACGCTTTGTC 
TCTGGACGGG ACTGCAGCCG CTGTCTGCCT GAGTTCTGGG GTCTGAGCAG TGACTCTCTA 
GGCTGTCGGC CCTGTGACTG TGACTTTGGG GGTGCCTACA GCAACAGGTG TTCTGCAGGG 
CAGGGCCTCT GCCTCTGCCG CCCCCACCTG CATGGCCGCC ACTGCCAGGA ACTCCAGTCT 
GGGTACTTCT GCGCCACCCT CGACCAGGCC ACTGCCGAAG CAGAGCATGG CCGCAGCCTC 
CAGCCTGCTG ACCCCCAGCT GCCTGGAGCC CCCTGGCCTG CTCCCCGCTA CTGTACACCA 
GCCCCAGGGA CCTCCTCTGG TTGGCTCGGG CCCCGACTTT GGAGACAGCG TGACCCTGAC 
TGTGTCCTCC AACGTGCCAG GCAAGCCCGG TACAGCCACC AGCTGCCCAG GACCCCAGGG 
AAGCCCCCGC CCCTGCATCA GGGATCTGGC TTTGCACTGG GGAGGGGCTC AGACTTCGCA 
CGTGTGGTGG ACGGGGCTGG CCTCTCTCTG CTGGCACCCA CAGTGCCTCG CGCCCTGGAC 
TATGACATCG TCCTACGCTA TGAGACCCAG GCACCTGAAG TGTGGCAGGC ATTAGTCCGG 
GTCCGTGCCC AGTCACAGCC CCGCAGTACC CGCTGTGCCC GTCCGTTGCC CTCAGAGCAG 
CTGTTCCAGG CGACCTTGAC CCATGCGCGC AGAGCCGTGG TTCTTTCCAG ACCGTTCTGC 
TTTGAACCCG GAACCCGCTA CTCCGTGACC CTGCGGCTGT GGCGGACCAA GGAGGGACGG 
AGACCCGAAG GGGGCACGAT CCTCCTGGAT TCAGTAGTGC TCCTGCCGCG GGTTGGAGAG 
TTGCCTGGAC TGAGGTCCGT GGACCCGGGG GCCCTGAGGC GCTTGCAGGA GCTCCACGAG 
GCGGGCTGCC TGGAGGCAGC GAGGGTGAGC CCACCCCAAA GCATGCCTGA GGCCTGTGCC 
CGCCTCGCCT GCAGCATCTC AGCCCTGCTC CATGGAGGTG GCCTCGCATG TGAGTGCCAC 
CTGCAGGGTT CACTGAGCAC CGAGTGTGCC CCACTGGGGG GGCAATGCCC CTGCCGCCCT 
AATATCACAG GTCGTACCTG TGACCGCTGC TTGCCTGGGA CATTTGGCCT CGGGCCCACC 
GGGTGCCATG AGTGCCGCTG CCACCCCGAG GGGGCTGCCA GTGCCGTCTG TAACTCTACG 
AGTGGGCAGT GCACGTGTTG GGCAGGCTGT GCTGGTCGCC GCTGTGACCG TTGCCTCTCT 
GGCCGGTGGG GCTTCCCACG CTGCCAGCCC TGTGCCTGCA ATGGACACGC CGAGTTGTGC 
CACCCACTCA CAGGCGTCTG CCAGGACTGC CGCGGAGCCA CCACAGGCCG GCATTGTGAG 
AGGTGCTTGG ATGGCTACTA TGGAGACCCC ACCCTGGGCT CAGGCCAGCG GTGCCAGCCC 
TGCCCCTGTC CTGGGCACCC TGGCTCAGGC CTCTATCATG GGACCTCCTG CCATGTGGAC 
AGCACCAGTG GACGTGTCCT GTGCCTCTGT GCCCCCGGCT ATGCAGGACC CCGCTGTGAC 
CGCTGCTCCC CCGGCTACTT TGGGCGCCCC TGGCCTGGAG ATGACCCCAG AAGGAGTCCC 
TGCCGGCCCT GCCAGTGCAA CAACAATATT GATCCCCGTG ACCCAGCCGC CTGTGACCCC 
CACAGCGGAC ATTGCCAGCG CTGTCTGCAC CACAGCCACG GCGCTGGCTG TGCCCACTGC 
CGGCCTGGCT TCCACGGCAG TGCCCTGCGC CCAGGGGGCT GCCGGCGCTG CAGCTGTGAC 
CCCCGGGGCA CCAGCCCTGT AAGGTGCCCA CCTGAGGCTG AAGCCTGCTT CTGTGACCCG 
GTCAGCGGGC AGTGCCCCTG CCGCCCCCAC ACACTGGGGC GGGACTGTAG CCGCTGTGCC 
CCCCTCTTCT GGAACCTCGG GGGGCCCCGG GGCTGTGAGC CCTGCAGCTG CCACGCCCGA 
CACACTTTGC AGCCGGGGTG TCACCCGGTC ACGGGTCAGT GCCCCTGCCG GGCAGGATTT 
GGGGGCCGCA CGTGCTCCCG GTGTCAGGAT GGGTACTGGG GTGACCCGGA GCAGGAGTGC 
AGAGCCTGTG CCTGTGACCC CCAGGGCTCC ATCTCGCCCA GCTGTGACCC GCACACAGGC 
ACCTGCCGCT GCCGAGAGGG TATCTCAGGG CCGAGGTGCC AGGCGTGTGC CCGTGGCTCC 
ACAGGAGGCT TCCCACACTG CACGTCCTGC CCTCCCTGCT TCGCCTCCTG GGACCAACGC 
CTGGCTCCCC TCCAGCTGCA TCTGGACACC ATGGTCCATG AGGTGGCTGC CCTGCGCCAG 
GGCATGCCTG GCTGGGGTGC TGGGCTCCGG GGAGATCAGC TGCAGGCCCT GGAGGGTAAA 
CTCCAGCAGG CTCAGACACT CCTGGAATCA CCTTCCCCCA CCAGAGGTCC CCTGCAACAA 
TTTACAGAGT GGATCACCGG ACTCAGGCAA GGGGTCTGGG GTCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315133.2
CDS	134..4141
Translation	MPDAGSHCPGAPAGLGPLLLALLLLLPPMVPAPRTSAQEVPDMMPPPGCSGGNCHPPTGNLVIGRGQSLRTSSTCGLHGPELYCVVSKLQALLQDSQNCCFCDSRDGKSHGIENVVSRSDPDGRKTWWQAESGVENVTIQLDLEGAFYFTHLIMTFKTFRPAALLLERSVDHGHSWHVFRYFAHNCSGLFPGIPPAPGRRVSDLVCDQRYSDIEPATEGEVIFQVLDPSFLAENTNNAEIQELLRVTNLRVNFSKLHTLGDRPLGGLRGHPFYYYALYELVIAGSCLCHGHASECRPAPGSPPSVEGMVHGHCVCRHHTTGTHCERCQGLYQDHPWQAAEPGYPHTCQECKCHGHARSCHFDMALYLASGNVSGGVCDACQHNTAGRHCELCQPFFHRDPLEDPRSLHSCKPCDCNPMGALEGGLCDAYTDTTRGLLSGQCRCKVHVWGQRCDSCRPGHYGLSLTQSEGCQPCRCNVRGRVPSTQACDPSSGACHCKRFVSGRDCSRCLPEFWGLSSDSLGCRPCDCDFGGAYSNRCSAGQGLCLCRPHLHGRHCQELQSGYFCATLDQATAEAEHGRSLQPADPQLPGAPWPAPRYCTPAPGTSSGWLGPRLWRQRDPDCVLQRARQARYSHQLPRTPGKPPPLHQGSGFALGRGSDFARVVDGAGLSLLAPTVPRALDYDIVLRYETQAPEVWQALVRVRAQSQPRSTRCARPLPSEQLFQATLTHARRAVVLSRPFCFEPGTRYSVTLRLWRTKEGRRPEGGTILLDSVVLLPRVGELPGLRSVDPGALRRLQELHEAGCLEAARVSPPQSMPEACARLACSISALLHGGGLACECHLQGSLSTECAPLGGQCPCRPNITGRTCDRCLPGTFGLGPTGCHECRCHPEGAASAVCNSTSGQCTCWAGCAGRRCDRCLSGRWGFPRCQPCACNGHAELCHPLTGVCQDCRGATTGRHCERCLDGYYGDPTLGSGQRCQPCPCPGHPGSGLYHGTSCHVDSTSGRVLCLCAPGYAGPRCDRCSPGYFGRPWPGDDPRRSPCRPCQCNNNIDPRDPAACDPHSGHCQRCLHHSHGAGCAHCRPGFHGSALRPGGCRRCSCDPRGTSPVRCPPEAEACFCDPVSGQCPCRPHTLGRDCSRCAPLFWNLGGPRGCEPCSCHARHTLQPGCHPVTGQCPCRAGFGGRTCSRCQDGYWGDPEQECRACACDPQGSISPSCDPHTGTCRCREGISGPRCQACARGSTGGFPHCTSCPPCFASWDQRLAPLQLHLDTMVHEVAALRQGMPGWGAGLRGDQLQALEGKLQQAQTLLESPSPTRGPLQQFTEWITGLRQGVWGH

Target ORF information:

RefSeq Version	XM_015459647.2
Organism	Bos taurus(cattle)
Definition	Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459647.2

ORF Insert Sequence:

ATGCCTGATG CTGGCTCCCA CTGCCCAGGA GCCCCAGCCG GGCTAGGCCC CCTTCTCTTA 
GCCCTGCTGC TGCTGCTGCC ACCCATGGTC CCGGCTCCCA GAACAAGTGC CCAGGAGGTA 
CCAGACATGA TGCCTCCTCC CGGCTGCTCA GGTGGCAACT GCCACCCACC TACTGGCAAC 
CTGGTTATTG GCCGTGGCCA GAGCCTTCGA ACCTCATCTA CCTGTGGCCT CCATGGCCCT 
GAACTCTACT GTGTTGTCAG TAAACTACAG GCGCTTCTCC AGGACTCTCA GAATTGCTGC 
TTTTGTGATT CTCGGGATGG GAAAAGCCAT GGCATTGAGA ATGTGGTCTC CCGGAGTGAC 
CCTGATGGCA GAAAGACCTG GTGGCAAGCA GAGAGTGGTG TTGAGAATGT CACCATCCAG 
TTGGACCTAG AAGGTGCCTT TTACTTTACC CACCTCATCA TGACTTTTAA GACATTCCGC 
CCAGCAGCTC TGCTCCTTGA GCGCTCAGTG GACCATGGCC ACTCCTGGCA TGTGTTCCGC 
TACTTTGCCC ACAACTGCTC AGGCCTCTTT CCTGGGATCC CTCCTGCCCC TGGCCGCAGA 
GTCAGTGACC TCGTCTGTGA CCAGCGTTAC TCAGACATCG AGCCTGCCAC TGAGGGAGAG 
GTAATTTTCC AAGTTCTGGA CCCATCCTTT CTGGCGGAGA ACACAAACAA CGCGGAGATT 
CAGGAACTCC TGCGTGTGAC TAACCTCCGT GTGAACTTCT CCAAGCTGCA CACACTGGGT 
GACAGGCCCC TGGGGGGCCT CAGGGGCCAC CCCTTCTACT ACTATGCTCT CTATGAGCTA 
GTGATTGCAG GCAGCTGCCT GTGCCACGGC CATGCTTCCG AGTGCAGGCC CGCACCCGGG 
TCCCCACCCA GCGTGGAAGG CATGGTGCAT GGCCATTGTG TCTGCCGCCA CCACACCACT 
GGTACCCACT GTGAACGTTG CCAGGGCCTG TACCAGGACC ACCCGTGGCA GGCAGCAGAG 
CCTGGGTACC CCCACACCTG CCAGGAATGC AAGTGCCATG GGCACGCCCG CAGTTGTCAC 
TTTGACATGG CCCTGTATCT CGCATCTGGC AACGTGAGTG GAGGTGTGTG CGATGCATGT 
CAACACAACA CGGCCGGGCG CCACTGTGAG CTCTGCCAGC CCTTCTTCCA CCGGGACCCC 
CTGGAGGACC CCCGCTCCCT TCACTCCTGC AAACCCTGTG ACTGCAACCC TATGGGTGCC 
CTGGAAGGGG GTTTGTGTGA TGCCTACACA GACACCACCC GGGGTCTCCT CTCTGGGCAG 
TGTCGCTGCA AAGTTCACGT CTGGGGCCAG CGCTGTGACT CCTGCCGGCC AGGTCACTAT 
GGCCTGAGCC TCACCCAGTC AGAAGGCTGC CAGCCCTGCA GGTGCAACGT CCGGGGCCGG 
GTCCCCAGCA CTCAGGCCTG TGATCCATCC AGCGGTGCCT GTCACTGCAA ACGCTTTGTC 
TCTGGACGGG ACTGCAGCCG CTGTCTGCCT GAGTTCTGGG GTCTGAGCAG TGACTCTCTA 
GGCTGTCGGC CCTGTGACTG TGACTTTGGG GGTGCCTACA GCAACAGGTG TTCTGCAGGG 
CAGGGCCTCT GCCTCTGCCG CCCCCACCTG CATGGCCGCC ACTGCCAGGA ACTCCAGTCT 
GGGTACTTCT GCGCCACCCT CGACCAGGCC ACTGCCGAAG CAGAGCATGG CCGCAGCCTC 
CAGCCTGCTG ACCCCCAGCT GCCTGGAGCC CCCTGGCCTG CTCCCCGCTA CTGTACACCA 
GCCCCAGGGA CCTCCTCTGG TTGGCTCGGG CCCCGACTTT GGAGACAGCG TGACCCTGAC 
TGTGTCCTCC AACGTGCCAG GCAAGCCCGG TACAGCCACC AGCTGCCCAG GACCCCAGGG 
AAGCCCCCGC CCCTGCATCA GGGATCTGGC TTTGCACTGG GGAGGGGCTC AGACTTCGCA 
CGTGTGGTGG ACGGGGCTGG CCTCTCTCTG CTGGCACCCA CAGTGCCTCG CGCCCTGGAC 
TATGACATCG TCCTACGCTA TGAGACCCAG GCACCTGAAG TGTGGCAGGC ATTAGTCCGG 
GTCCGTGCCC AGTCACAGCC CCGCAGTACC CGCTGTGCCC GTCCGTTGCC CTCAGAGCAG 
CTGTTCCAGG CGACCTTGAC CCATGCGCGC AGAGCCGTGG TTCTTTCCAG ACCGTTCTGC 
TTTGAACCCG GAACCCGCTA CTCCGTGACC CTGCGGCTGT GGCGGACCAA GGAGGGACGG 
AGACCCGAAG GGGGCACGAT CCTCCTGGAT TCAGTAGTGC TCCTGCCGCG GGTTGGAGAG 
TTGCCTGGAC TGAGGTCCGT GGACCCGGGG GCCCTGAGGC GCTTGCAGGA GCTCCACGAG 
GCGGGCTGCC TGGAGGCAGC GAGGGTGAGC CCACCCCAAA GCATGCCTGA GGCCTGTGCC 
CGCCTCGCCT GCAGCATCTC AGCCCTGCTC CATGGAGGTG GCCTCGCATG TGAGTGCCAC 
CTGCAGGGTT CACTGAGCAC CGAGTGTGCC CCACTGGGGG GGCAATGCCC CTGCCGCCCT 
AATATCACAG GTCGTACCTG TGACCGCTGC TTGCCTGGGA CATTTGGCCT CGGGCCCACC 
GGGTGCCATG AGTGCCGCTG CCACCCCGAG GGGGCTGCCA GTGCCGTCTG TAACTCTACG 
AGTGGGCAGT GCACGTGTTG GGCAGGCTGT GCTGGTCGCC GCTGTGACCG TTGCCTCTCT 
GGCCGGTGGG GCTTCCCACG CTGCCAGCCC TGTGCCTGCA ATGGACACGC CGAGTTGTGC 
CACCCACTCA CAGGCGTCTG CCAGGACTGC CGCGGAGCCA CCACAGGCCG GCATTGTGAG 
AGGTGCTTGG ATGGCTACTA TGGAGACCCC ACCCTGGGCT CAGGCCAGCG GTGCCAGCCC 
TGCCCCTGTC CTGGGCACCC TGGCTCAGGC CTCTATCATG GGACCTCCTG CCATGTGGAC 
AGCACCAGTG GACGTGTCCT GTGCCTCTGT GCCCCCGGCT ATGCAGGACC CCGCTGTGAC 
CGCTGCTCCC CCGGCTACTT TGGGCGCCCC TGGCCTGGAG ATGACCCCAG AAGGAGTCCC 
TGCCGGCCCT GCCAGTGCAA CAACAATATT GATCCCCGTG ACCCAGCCGC CTGTGACCCC 
CACAGCGGAC ATTGCCAGCG CTGTCTGCAC CACAGCCACG GCGCTGGCTG TGCCCACTGC 
CGGCCTGGCT TCCACGGCAG TGCCCTGCGC CCAGGGGGCT GCCGGCGCTG CAGCTGTGAC 
CCCCGGGGCA CCAGCCCTGT AAGGTGCCCA CCTGAGGCTG AAGCCTGCTT CTGTGACCCG 
GTCAGCGGGC AGTGCCCCTG CCGCCCCCAC ACACTGGGGC GGGACTGTAG CCGCTGTGCC 
CCCCTCTTCT GGAACCTCGG GGGGCCCCGG GGCTGTGAGC CCTGCAGCTG CCACGCCCGA 
CACACTTTGC AGCCGGGGTG TCACCCGGTC ACGGGTCAGT GCCCCTGCCG GGCAGGATTT 
GGGGGCCGCA CGTGCTCCCG GTGTCAGGAT GGGTACTGGG GTGACCCGGA GCAGGAGTGC 
AGAGCCTGTG CCTGTGACCC CCAGGGCTCC ATCTCGCCCA GCTGTGACCC GCACACAGGC 
ACCTGCCGCT GCCGAGAGGG TATCTCAGGG CCGAGGTGCC AGGCGTGTGC CCGTGGCTCC 
ACAGGAGGCT TCCCACACTG CACGTCCTGC CCTCCCTGCT TCGCCTCCTG GGACCAACGC 
CTGGCTCCCC TCCAGCTGCA TCTGGACACC ATGGTCCATG AGGTGGCTGC CCTGCGCCAG 
GGCATGCCTG GCTGGGGTGC TGGGCTCCGG GGAGATCAGC TGCAGGCCCT GGAGGGTAAA 
CTCCAGCAGG CTCAGACACT CCTGGAATCA CCTTCCCCCA CCAGAGGTCC CCTGCAACAA 
TTTACAGAGT GGATCACCGG ACTCAGGCAA GGGGTCTGGG GTCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC530599 ( XM_015459648.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315134.2 Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa229391
Clone ID Related Accession (Same CDS sequence)	XM_015459648.2
Accession Version	XM_015459648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	laminin subunit beta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459648.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATG CTGGCTCCCA CTGCCCAGGA GCCCCAGCCG GGCTAGGCCC CCTTCTCTTA 
GCCCTGCTGC TGCTGCTGCC ACCCATGGTC CCGGCTCCCA GAACAAGTGC CCAGGAGGTA 
CCAGACATGA TGCCTCCTCC CGGCTGCTCA GGTGGCAACT GCCACCCACC TACTGGCAAC 
CTGGTTATTG GCCGTGGCCA GAGCCTTCGA ACCTCATCTA CCTGTGGCCT CCATGGCCCT 
GAACTCTACT GTGTTGTCAG TAAACTACAG GACTCTCAGA ATTGCTGCTT TTGTGATTCT 
CGGGATGGGA AAAGCCATGG CATTGAGAAT GTGGTCTCCC GGAGTGACCC TGATGGCAGA 
AAGACCTGGT GGCAAGCAGA GAGTGGTGTT GAGAATGTCA CCATCCAGTT GGACCTAGAA 
GGTGCCTTTT ACTTTACCCA CCTCATCATG ACTTTTAAGA CATTCCGCCC AGCAGCTCTG 
CTCCTTGAGC GCTCAGTGGA CCATGGCCAC TCCTGGCATG TGTTCCGCTA CTTTGCCCAC 
AACTGCTCAG GCCTCTTTCC TGGGATCCCT CCTGCCCCTG GCCGCAGAGT CAGTGACCTC 
GTCTGTGACC AGCGTTACTC AGACATCGAG CCTGCCACTG AGGGAGAGGT AATTTTCCAA 
GTTCTGGACC CATCCTTTCT GGCGGAGAAC ACAAACAACG CGGAGATTCA GGAACTCCTG 
CGTGTGACTA ACCTCCGTGT GAACTTCTCC AAGCTGCACA CACTGGGTGA CAGGCCCCTG 
GGGGGCCTCA GGGGCCACCC CTTCTACTAC TATGCTCTCT ATGAGCTAGT GATTGCAGGC 
AGCTGCCTGT GCCACGGCCA TGCTTCCGAG TGCAGGCCCG CACCCGGGTC CCCACCCAGC 
GTGGAAGGCA TGGTGCATGG CCATTGTGTC TGCCGCCACC ACACCACTGG TACCCACTGT 
GAACGTTGCC AGGGCCTGTA CCAGGACCAC CCGTGGCAGG CAGCAGAGCC TGGGTACCCC 
CACACCTGCC AGGAATGCAA GTGCCATGGG CACGCCCGCA GTTGTCACTT TGACATGGCC 
CTGTATCTCG CATCTGGCAA CGTGAGTGGA GGTGTGTGCG ATGCATGTCA ACACAACACG 
GCCGGGCGCC ACTGTGAGCT CTGCCAGCCC TTCTTCCACC GGGACCCCCT GGAGGACCCC 
CGCTCCCTTC ACTCCTGCAA ACCCTGTGAC TGCAACCCTA TGGGTGCCCT GGAAGGGGGT 
TTGTGTGATG CCTACACAGA CACCACCCGG GGTCTCCTCT CTGGGCAGTG TCGCTGCAAA 
GTTCACGTCT GGGGCCAGCG CTGTGACTCC TGCCGGCCAG GTCACTATGG CCTGAGCCTC 
ACCCAGTCAG AAGGCTGCCA GCCCTGCAGG TGCAACGTCC GGGGCCGGGT CCCCAGCACT 
CAGGCCTGTG ATCCATCCAG CGGTGCCTGT CACTGCAAAC GCTTTGTCTC TGGACGGGAC 
TGCAGCCGCT GTCTGCCTGA GTTCTGGGGT CTGAGCAGTG ACTCTCTAGG CTGTCGGCCC 
TGTGACTGTG ACTTTGGGGG TGCCTACAGC AACAGGTGTT CTGCAGGGCA GGGCCTCTGC 
CTCTGCCGCC CCCACCTGCA TGGCCGCCAC TGCCAGGAAC TCCAGTCTGG GTACTTCTGC 
GCCACCCTCG ACCAGGCCAC TGCCGAAGCA GAGCATGGCC GCAGCCTCCA GCCTGCTGAC 
CCCCAGCTGC CTGGAGCCCC CTGGCCTGCT CCCCGCTACT GTACACCAGC CCCAGGGACC 
TCCTCTGGTT GGCTCGGGCC CCGACTTTGG AGACAGCGTG ACCCTGACTG TGTCCTCCAA 
CGTGCCAGGC AAGCCCGGTA CAGCCACCAG CTGCCCAGGA CCCCAGGGAA GCCCCCGCCC 
CTGCATCAGG GATCTGGCTT TGCACTGGGG AGGGGCTCAG ACTTCGCACG TGTGGTGGAC 
GGGGCTGGCC TCTCTCTGCT GGCACCCACA GTGCCTCGCG CCCTGGACTA TGACATCGTC 
CTACGCTATG AGACCCAGGC ACCTGAAGTG TGGCAGGCAT TAGTCCGGGT CCGTGCCCAG 
TCACAGCCCC GCAGTACCCG CTGTGCCCGT CCGTTGCCCT CAGAGCAGCT GTTCCAGGCG 
ACCTTGACCC ATGCGCGCAG AGCCGTGGTT CTTTCCAGAC CGTTCTGCTT TGAACCCGGA 
ACCCGCTACT CCGTGACCCT GCGGCTGTGG CGGACCAAGG AGGGACGGAG ACCCGAAGGG 
GGCACGATCC TCCTGGATTC AGTAGTGCTC CTGCCGCGGG TTGGAGAGTT GCCTGGACTG 
AGGTCCGTGG ACCCGGGGGC CCTGAGGCGC TTGCAGGAGC TCCACGAGGC GGGCTGCCTG 
GAGGCAGCGA GGGTGAGCCC ACCCCAAAGC ATGCCTGAGG CCTGTGCCCG CCTCGCCTGC 
AGCATCTCAG CCCTGCTCCA TGGAGGTGGC CTCGCATGTG AGTGCCACCT GCAGGGTTCA 
CTGAGCACCG AGTGTGCCCC ACTGGGGGGG CAATGCCCCT GCCGCCCTAA TATCACAGGT 
CGTACCTGTG ACCGCTGCTT GCCTGGGACA TTTGGCCTCG GGCCCACCGG GTGCCATGAG 
TGCCGCTGCC ACCCCGAGGG GGCTGCCAGT GCCGTCTGTA ACTCTACGAG TGGGCAGTGC 
ACGTGTTGGG CAGGCTGTGC TGGTCGCCGC TGTGACCGTT GCCTCTCTGG CCGGTGGGGC 
TTCCCACGCT GCCAGCCCTG TGCCTGCAAT GGACACGCCG AGTTGTGCCA CCCACTCACA 
GGCGTCTGCC AGGACTGCCG CGGAGCCACC ACAGGCCGGC ATTGTGAGAG GTGCTTGGAT 
GGCTACTATG GAGACCCCAC CCTGGGCTCA GGCCAGCGGT GCCAGCCCTG CCCCTGTCCT 
GGGCACCCTG GCTCAGGCCT CTATCATGGG ACCTCCTGCC ATGTGGACAG CACCAGTGGA 
CGTGTCCTGT GCCTCTGTGC CCCCGGCTAT GCAGGACCCC GCTGTGACCG CTGCTCCCCC 
GGCTACTTTG GGCGCCCCTG GCCTGGAGAT GACCCCAGAA GGAGTCCCTG CCGGCCCTGC 
CAGTGCAACA ACAATATTGA TCCCCGTGAC CCAGCCGCCT GTGACCCCCA CAGCGGACAT 
TGCCAGCGCT GTCTGCACCA CAGCCACGGC GCTGGCTGTG CCCACTGCCG GCCTGGCTTC 
CACGGCAGTG CCCTGCGCCC AGGGGGCTGC CGGCGCTGCA GCTGTGACCC CCGGGGCACC 
AGCCCTGTAA GGTGCCCACC TGAGGCTGAA GCCTGCTTCT GTGACCCGGT CAGCGGGCAG 
TGCCCCTGCC GCCCCCACAC ACTGGGGCGG GACTGTAGCC GCTGTGCCCC CCTCTTCTGG 
AACCTCGGGG GGCCCCGGGG CTGTGAGCCC TGCAGCTGCC ACGCCCGACA CACTTTGCAG 
CCGGGGTGTC ACCCGGTCAC GGGTCAGTGC CCCTGCCGGG CAGGATTTGG GGGCCGCACG 
TGCTCCCGGT GTCAGGATGG GTACTGGGGT GACCCGGAGC AGGAGTGCAG AGCCTGTGCC 
TGTGACCCCC AGGGCTCCAT CTCGCCCAGC TGTGACCCGC ACACAGGCAC CTGCCGCTGC 
CGAGAGGGTA TCTCAGGGCC GAGGTGCCAG GCGTGTGCCC GTGGCTCCAC AGGAGGCTTC 
CCACACTGCA CGTCCTGCCC TCCCTGCTTC GCCTCCTGGG ACCAACGCCT GGCTCCCCTC 
CAGCTGCATC TGGACACCAT GGTCCATGAG GTGGCTGCCC TGCGCCAGGG CATGCCTGGC 
TGGGGTGCTG GGCTCCGGGG AGATCAGCTG CAGGCCCTGG AGGGTAAACT CCAGCAGGCT 
CAGACACTCC TGGAATCACC TTCCCCCACC AGAGGTCCCC TGCAACAATT TACAGAGTGG 
ATCACCGGAC TCAGGCAAGG GGTCTGGGGT CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315134.2
CDS	134..4129
Translation	MPDAGSHCPGAPAGLGPLLLALLLLLPPMVPAPRTSAQEVPDMMPPPGCSGGNCHPPTGNLVIGRGQSLRTSSTCGLHGPELYCVVSKLQDSQNCCFCDSRDGKSHGIENVVSRSDPDGRKTWWQAESGVENVTIQLDLEGAFYFTHLIMTFKTFRPAALLLERSVDHGHSWHVFRYFAHNCSGLFPGIPPAPGRRVSDLVCDQRYSDIEPATEGEVIFQVLDPSFLAENTNNAEIQELLRVTNLRVNFSKLHTLGDRPLGGLRGHPFYYYALYELVIAGSCLCHGHASECRPAPGSPPSVEGMVHGHCVCRHHTTGTHCERCQGLYQDHPWQAAEPGYPHTCQECKCHGHARSCHFDMALYLASGNVSGGVCDACQHNTAGRHCELCQPFFHRDPLEDPRSLHSCKPCDCNPMGALEGGLCDAYTDTTRGLLSGQCRCKVHVWGQRCDSCRPGHYGLSLTQSEGCQPCRCNVRGRVPSTQACDPSSGACHCKRFVSGRDCSRCLPEFWGLSSDSLGCRPCDCDFGGAYSNRCSAGQGLCLCRPHLHGRHCQELQSGYFCATLDQATAEAEHGRSLQPADPQLPGAPWPAPRYCTPAPGTSSGWLGPRLWRQRDPDCVLQRARQARYSHQLPRTPGKPPPLHQGSGFALGRGSDFARVVDGAGLSLLAPTVPRALDYDIVLRYETQAPEVWQALVRVRAQSQPRSTRCARPLPSEQLFQATLTHARRAVVLSRPFCFEPGTRYSVTLRLWRTKEGRRPEGGTILLDSVVLLPRVGELPGLRSVDPGALRRLQELHEAGCLEAARVSPPQSMPEACARLACSISALLHGGGLACECHLQGSLSTECAPLGGQCPCRPNITGRTCDRCLPGTFGLGPTGCHECRCHPEGAASAVCNSTSGQCTCWAGCAGRRCDRCLSGRWGFPRCQPCACNGHAELCHPLTGVCQDCRGATTGRHCERCLDGYYGDPTLGSGQRCQPCPCPGHPGSGLYHGTSCHVDSTSGRVLCLCAPGYAGPRCDRCSPGYFGRPWPGDDPRRSPCRPCQCNNNIDPRDPAACDPHSGHCQRCLHHSHGAGCAHCRPGFHGSALRPGGCRRCSCDPRGTSPVRCPPEAEACFCDPVSGQCPCRPHTLGRDCSRCAPLFWNLGGPRGCEPCSCHARHTLQPGCHPVTGQCPCRAGFGGRTCSRCQDGYWGDPEQECRACACDPQGSISPSCDPHTGTCRCREGISGPRCQACARGSTGGFPHCTSCPPCFASWDQRLAPLQLHLDTMVHEVAALRQGMPGWGAGLRGDQLQALEGKLQQAQTLLESPSPTRGPLQQFTEWITGLRQGVWGH

Target ORF information:

RefSeq Version	XM_015459648.2
Organism	Bos taurus(cattle)
Definition	Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459648.2

ORF Insert Sequence:

ATGCCTGATG CTGGCTCCCA CTGCCCAGGA GCCCCAGCCG GGCTAGGCCC CCTTCTCTTA 
GCCCTGCTGC TGCTGCTGCC ACCCATGGTC CCGGCTCCCA GAACAAGTGC CCAGGAGGTA 
CCAGACATGA TGCCTCCTCC CGGCTGCTCA GGTGGCAACT GCCACCCACC TACTGGCAAC 
CTGGTTATTG GCCGTGGCCA GAGCCTTCGA ACCTCATCTA CCTGTGGCCT CCATGGCCCT 
GAACTCTACT GTGTTGTCAG TAAACTACAG GACTCTCAGA ATTGCTGCTT TTGTGATTCT 
CGGGATGGGA AAAGCCATGG CATTGAGAAT GTGGTCTCCC GGAGTGACCC TGATGGCAGA 
AAGACCTGGT GGCAAGCAGA GAGTGGTGTT GAGAATGTCA CCATCCAGTT GGACCTAGAA 
GGTGCCTTTT ACTTTACCCA CCTCATCATG ACTTTTAAGA CATTCCGCCC AGCAGCTCTG 
CTCCTTGAGC GCTCAGTGGA CCATGGCCAC TCCTGGCATG TGTTCCGCTA CTTTGCCCAC 
AACTGCTCAG GCCTCTTTCC TGGGATCCCT CCTGCCCCTG GCCGCAGAGT CAGTGACCTC 
GTCTGTGACC AGCGTTACTC AGACATCGAG CCTGCCACTG AGGGAGAGGT AATTTTCCAA 
GTTCTGGACC CATCCTTTCT GGCGGAGAAC ACAAACAACG CGGAGATTCA GGAACTCCTG 
CGTGTGACTA ACCTCCGTGT GAACTTCTCC AAGCTGCACA CACTGGGTGA CAGGCCCCTG 
GGGGGCCTCA GGGGCCACCC CTTCTACTAC TATGCTCTCT ATGAGCTAGT GATTGCAGGC 
AGCTGCCTGT GCCACGGCCA TGCTTCCGAG TGCAGGCCCG CACCCGGGTC CCCACCCAGC 
GTGGAAGGCA TGGTGCATGG CCATTGTGTC TGCCGCCACC ACACCACTGG TACCCACTGT 
GAACGTTGCC AGGGCCTGTA CCAGGACCAC CCGTGGCAGG CAGCAGAGCC TGGGTACCCC 
CACACCTGCC AGGAATGCAA GTGCCATGGG CACGCCCGCA GTTGTCACTT TGACATGGCC 
CTGTATCTCG CATCTGGCAA CGTGAGTGGA GGTGTGTGCG ATGCATGTCA ACACAACACG 
GCCGGGCGCC ACTGTGAGCT CTGCCAGCCC TTCTTCCACC GGGACCCCCT GGAGGACCCC 
CGCTCCCTTC ACTCCTGCAA ACCCTGTGAC TGCAACCCTA TGGGTGCCCT GGAAGGGGGT 
TTGTGTGATG CCTACACAGA CACCACCCGG GGTCTCCTCT CTGGGCAGTG TCGCTGCAAA 
GTTCACGTCT GGGGCCAGCG CTGTGACTCC TGCCGGCCAG GTCACTATGG CCTGAGCCTC 
ACCCAGTCAG AAGGCTGCCA GCCCTGCAGG TGCAACGTCC GGGGCCGGGT CCCCAGCACT 
CAGGCCTGTG ATCCATCCAG CGGTGCCTGT CACTGCAAAC GCTTTGTCTC TGGACGGGAC 
TGCAGCCGCT GTCTGCCTGA GTTCTGGGGT CTGAGCAGTG ACTCTCTAGG CTGTCGGCCC 
TGTGACTGTG ACTTTGGGGG TGCCTACAGC AACAGGTGTT CTGCAGGGCA GGGCCTCTGC 
CTCTGCCGCC CCCACCTGCA TGGCCGCCAC TGCCAGGAAC TCCAGTCTGG GTACTTCTGC 
GCCACCCTCG ACCAGGCCAC TGCCGAAGCA GAGCATGGCC GCAGCCTCCA GCCTGCTGAC 
CCCCAGCTGC CTGGAGCCCC CTGGCCTGCT CCCCGCTACT GTACACCAGC CCCAGGGACC 
TCCTCTGGTT GGCTCGGGCC CCGACTTTGG AGACAGCGTG ACCCTGACTG TGTCCTCCAA 
CGTGCCAGGC AAGCCCGGTA CAGCCACCAG CTGCCCAGGA CCCCAGGGAA GCCCCCGCCC 
CTGCATCAGG GATCTGGCTT TGCACTGGGG AGGGGCTCAG ACTTCGCACG TGTGGTGGAC 
GGGGCTGGCC TCTCTCTGCT GGCACCCACA GTGCCTCGCG CCCTGGACTA TGACATCGTC 
CTACGCTATG AGACCCAGGC ACCTGAAGTG TGGCAGGCAT TAGTCCGGGT CCGTGCCCAG 
TCACAGCCCC GCAGTACCCG CTGTGCCCGT CCGTTGCCCT CAGAGCAGCT GTTCCAGGCG 
ACCTTGACCC ATGCGCGCAG AGCCGTGGTT CTTTCCAGAC CGTTCTGCTT TGAACCCGGA 
ACCCGCTACT CCGTGACCCT GCGGCTGTGG CGGACCAAGG AGGGACGGAG ACCCGAAGGG 
GGCACGATCC TCCTGGATTC AGTAGTGCTC CTGCCGCGGG TTGGAGAGTT GCCTGGACTG 
AGGTCCGTGG ACCCGGGGGC CCTGAGGCGC TTGCAGGAGC TCCACGAGGC GGGCTGCCTG 
GAGGCAGCGA GGGTGAGCCC ACCCCAAAGC ATGCCTGAGG CCTGTGCCCG CCTCGCCTGC 
AGCATCTCAG CCCTGCTCCA TGGAGGTGGC CTCGCATGTG AGTGCCACCT GCAGGGTTCA 
CTGAGCACCG AGTGTGCCCC ACTGGGGGGG CAATGCCCCT GCCGCCCTAA TATCACAGGT 
CGTACCTGTG ACCGCTGCTT GCCTGGGACA TTTGGCCTCG GGCCCACCGG GTGCCATGAG 
TGCCGCTGCC ACCCCGAGGG GGCTGCCAGT GCCGTCTGTA ACTCTACGAG TGGGCAGTGC 
ACGTGTTGGG CAGGCTGTGC TGGTCGCCGC TGTGACCGTT GCCTCTCTGG CCGGTGGGGC 
TTCCCACGCT GCCAGCCCTG TGCCTGCAAT GGACACGCCG AGTTGTGCCA CCCACTCACA 
GGCGTCTGCC AGGACTGCCG CGGAGCCACC ACAGGCCGGC ATTGTGAGAG GTGCTTGGAT 
GGCTACTATG GAGACCCCAC CCTGGGCTCA GGCCAGCGGT GCCAGCCCTG CCCCTGTCCT 
GGGCACCCTG GCTCAGGCCT CTATCATGGG ACCTCCTGCC ATGTGGACAG CACCAGTGGA 
CGTGTCCTGT GCCTCTGTGC CCCCGGCTAT GCAGGACCCC GCTGTGACCG CTGCTCCCCC 
GGCTACTTTG GGCGCCCCTG GCCTGGAGAT GACCCCAGAA GGAGTCCCTG CCGGCCCTGC 
CAGTGCAACA ACAATATTGA TCCCCGTGAC CCAGCCGCCT GTGACCCCCA CAGCGGACAT 
TGCCAGCGCT GTCTGCACCA CAGCCACGGC GCTGGCTGTG CCCACTGCCG GCCTGGCTTC 
CACGGCAGTG CCCTGCGCCC AGGGGGCTGC CGGCGCTGCA GCTGTGACCC CCGGGGCACC 
AGCCCTGTAA GGTGCCCACC TGAGGCTGAA GCCTGCTTCT GTGACCCGGT CAGCGGGCAG 
TGCCCCTGCC GCCCCCACAC ACTGGGGCGG GACTGTAGCC GCTGTGCCCC CCTCTTCTGG 
AACCTCGGGG GGCCCCGGGG CTGTGAGCCC TGCAGCTGCC ACGCCCGACA CACTTTGCAG 
CCGGGGTGTC ACCCGGTCAC GGGTCAGTGC CCCTGCCGGG CAGGATTTGG GGGCCGCACG 
TGCTCCCGGT GTCAGGATGG GTACTGGGGT GACCCGGAGC AGGAGTGCAG AGCCTGTGCC 
TGTGACCCCC AGGGCTCCAT CTCGCCCAGC TGTGACCCGC ACACAGGCAC CTGCCGCTGC 
CGAGAGGGTA TCTCAGGGCC GAGGTGCCAG GCGTGTGCCC GTGGCTCCAC AGGAGGCTTC 
CCACACTGCA CGTCCTGCCC TCCCTGCTTC GCCTCCTGGG ACCAACGCCT GGCTCCCCTC 
CAGCTGCATC TGGACACCAT GGTCCATGAG GTGGCTGCCC TGCGCCAGGG CATGCCTGGC 
TGGGGTGCTG GGCTCCGGGG AGATCAGCTG CAGGCCCTGG AGGGTAAACT CCAGCAGGCT 
CAGACACTCC TGGAATCACC TTCCCCCACC AGAGGTCCCC TGCAACAATT TACAGAGTGG 
ATCACCGGAC TCAGGCAAGG GGTCTGGGGT CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC530599 ( XM_024983358.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839126.1 Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X3, mRNA.

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CloneID	OBa229392
Clone ID Related Accession (Same CDS sequence)	XM_024983358.1
Accession Version	XM_024983358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	laminin subunit beta-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATG CTGGCTCCCA CTGCCCAGGA GCCCCAGCCG GGCTAGGCCC CCTTCTCTTA 
GCCCTGCTGC TGCTGCTGCC ACCCATGGTC CCGGCTCCCA GAACAAGTGC CCAGGAGGTA 
CCAGACATGA TGCCTCCTCC CGGCTGCTCA GGTGGCAACT GCCACCCACC TACTGGCAAC 
CTGGTTATTG GCCGTGGCCA GAGCCTTCGA ACCTCATCTA CCTGTGGCCT CCATGGCCCT 
GAACTCTACT GTGTTGTCAG TAAACTACAG GCGCTTCTCC AGGACTCTCA GAATTGCTGC 
TTTTGTGATT CTCGGGATGG GAAAAGCCAT GGCATTGAGA ATGTGGTCTC CCGGAGTGAC 
CCTGATGGCA GAAAGACCTG GTGGCAAGCA GAGAGTGGTG TTGAGAATGT CACCATCCAG 
TTGGACCTAG AAGGTGCCTT TTACTTTACC CACCTCATCA TGACTTTTAA GACATTCCGC 
CCAGCAGCTC TGCTCCTTGA GCGCTCAGTG GACCATGGCC ACTCCTGGCA TGTGTTCCGC 
TACTTTGCCC ACAACTGCTC AGGCCTCTTT CCTGGGATCC CTCCTGCCCC TGGCCGCAGA 
GTCAGTGACC TCGTCTGTGA CCAGCGTTAC TCAGACATCG AGCCTGCCAC TGAGGGAGAG 
GTAATTTTCC AAGTTCTGGA CCCATCCTTT CTGGCGGAGA ACACAAACAA CGCGGAGATT 
CAGGAACTCC TGCGTGTGAC TAACCTCCGT GTGAACTTCT CCAAGCTGCA CACACTGGGT 
GACAGGCCCC TGGGGGGCCT CAGGGGCCAC CCCTTCTACT ACTATGCTCT CTATGAGCTA 
GTGATTGCAG GCAGCTGCCT GTGCCACGGC CATGCTTCCG AGTGCAGGCC CGCACCCGGG 
TCCCCACCCA GCGTGGAAGG CATGGTGCAT GGCCATTGTG TCTGCCGCCA CCACACCACT 
GGTACCCACT GTGAACGTTG CCAGGGCCTG TACCAGGACC ACCCGTGGCA GGCAGCAGAG 
CCTGGGTACC CCCACACCTG CCAGGAATGC AAGTGCCATG GGCACGCCCG CAGTTGTCAC 
TTTGACATGG CCCTGTATCT CGCATCTGGC AACGTGAGTG GAGGTGTGTG CGATGCATGT 
CAACACAACA CGGCCGGGCG CCACTGTGAG CTCTGCCAGC CCTTCTTCCA CCGGGACCCC 
CTGGAGGACC CCCGCTCCCT TCACTCCTGC AAACCCTGTG ACTGCAACCC TATGGGTGCC 
CTGGAAGGGG GTTTGTGTGA TGCCTACACA GACACCACCC GGGGTCTCCT CTCTGGGCAG 
TGTCGCTGCA AAGTTCACGT CTGGGGCCAG CGCTGTGACT CCTGCCGGCC AGGTCACTAT 
GGCCTGAGCC TCACCCAGTC AGAAGGCTGC CAGCCCTGCA GGTGCAACGT CCGGGGCCGG 
GTCCCCAGCA CTCAGGCCTG TGATCCATCC AGCGGTGCCT GTCACTGCAA ACGCTTTGTC 
TCTGGACGGG ACTGCAGCCG CTGTCTGCCT GAGTTCTGGG GTCTGAGCAG TGACTCTCTA 
GGCTGTCGGC CCTGTGACTG TGACTTTGGG GGTGCCTACA GCAACAGGTG TTCTGCAGGG 
CAGGGCCTCT GCCTCTGCCG CCCCCACCTG CATGGCCGCC ACTGCCAGGA ACTCCAGTCT 
GGGTACTTCT GCGCCACCCT CGACCAGGCC ACTGCCGAAG CAGAGCATGG CCGCAGCCTC 
CAGCCTGCTG ACCCCCAGCT GCCTGGAGCC CCCTGGCCTG CTCCCCGCTA CTGTACACCA 
GCCCCAGGGA CCTCCTCTGG TTGGCTCGGG CCCCGACTTT GGAGACAGCG TGACCCTGAC 
TGTGTCCTCC AACGTGCCAG GCAAGCCCGG TACAGCCACC AGCTGCCCAG GACCCCAGGG 
AAGCCCCCGC CCCTGCATCA GGGATCTGGC TTTGCACTGG GGAGGGGCTC AGACTTCGCA 
CGTGTGGTGG ACGGGGCTGG CCTCTCTCTG CTGGCACCCA CAGTGCCTCG CGCCCTGGAC 
TATGACATCG TCCTACGCTA TGAGACCCAG GCACCTGAAG TGTGGCAGGC ATTAGTCCGG 
GTCCGTGCCC AGTCACAGCC CCGCAGTACC CGCTGTGCCC GTCCGTTGCC CTCAGAGCAG 
CTGTTCCAGG CGACCTTGAC CCATGCGCGC AGAGCCGTGG TTCTTTCCAG ACCGTTCTGC 
TTTGAACCCG GAACCCGCTA CTCCGTGACC CTGCGGCTGT GGCGGACCAA GGAGGGACGG 
AGACCCGAAG GGGGCACGAT CCTCCTGGAT TCAGTAGTGC TCCTGCCGCG GGTTGGAGAG 
TTGCCTGGAC TGAGGTCCGT GGACCCGGGG GCCCTGAGGC GCTTGCAGGA GCTCCACGAG 
GCGGGCTGCC TGGAGGCAGC GAGGGTGAGC CCACCCCAAA GCATGCCTGA GGCCTGTGCC 
CGCCTCGCCT GCAGCATCTC AGCCCTGCTC CATGGAGGTG GCCTCGCATG TGAGTGCCAC 
CTGCAGGGTT CACTGAGCAC CGAGTGTGCC CCACTGGGGG GGCAATGCCC CTGCCGCCCT 
AATATCACAG GTCGTACCTG TGACCGCTGC TTGCCTGGGA CATTTGGCCT CGGGCCCACC 
GGGTGCCATG AGTGCCGCTG CCACCCCGAG GGGGCTGCCA GTGCCGTCTG TAACTCTACG 
AGTGGGCAGT GCACGTGTTG GGCAGGCTGT GCTGGTCGCC GCTGTGACCG TTGCCTCTCT 
GGCCGGTGGG GCTTCCCACG CTGCCAGCCC TGTGCCTGCA ATGGACACGC CGAGTTGTGC 
CACCCACTCA CAGGCGTCTG CCAGGACTGC CGCGGAGCCA CCACAGGCCG GCATTGTGAG 
AGGTGCTTGG ATGGCTACTA TGGAGACCCC ACCCTGGGCT CAGGCCAGCG GTGCCAGCCC 
TGCCCCTGTC CTGGGCACCC TGGCTCAGGC CTCTATCATG GGACCTCCTG CCATGTGGAC 
AGCACCAGTG GACGTGTCCT GTGCCTCTGT GCCCCCGGCT ATGCAGGACC CCGCTGTGAC 
CGCTGCTCCC CCGGCTACTT TGGGCGCCCC TGGCCTGGAG ATGACCCCAG AAGGAGTCCC 
TGCCGGCCCT GCCAGTGCAA CAACAATATT GATCCCCGTG ACCCAGCCGC CTGTGACCCC 
CACAGCGGAC ATTGCCAGCG CTGTCTGCAC CACAGCCACG GCGCTGGCTG TGCCCACTGC 
CGGCCTGGCT TCCACGGCAG TGCCCTGCGC CCAGGGGGCT GCCGGCGCTG CAGCTGTGAC 
CCCCGGGGCA CCAGCCCTGT AAGGTGCCCA CCTGAGGCTG AAGCCTGCTT CTGTGACCCG 
GTCAGCGGGC AGTGCCCCTG CCGCCCCCAC ACACTGGGGC GGGACTGTAG CCGCTGTGCC 
CCCCTCTTCT GGAACCTCGG GGGGCCCCGG GGCTGTGAGC CCTGCAGCTG CCACGCCCGA 
CACACTTTGC AGCCGGGGTG TCACCCGGTC ACGGGTCAGT GCCCCTGCCG GGCAGGATTT 
GGGGGCCGCA CGTGCTCCCG GTGTCAGGAT GGGTACTGGG GTGACCCGGA GCAGGAGTGC 
AGAGCCTGTG CCTGTGACCC CCAGGGCTCC ATCTCGCCCA GCTGTGACCC GCACACAGGC 
ACCTGCCGCT GCCGAGAGGG TATCTCAGGG CCGAGGTGCC AGGCGTGTGC CCGTGGCTCC 
ACAGGAGGCT TCCCACACTG CACGTCCTGC CCTCCCTGCT TCGCCTCCTG GGACCAACGC 
CTGGCTCCCC TCCAGCTGCA TCTGGACACC ATGGTCCATG AGGACAGAAA GAACCACATT 
GGGCCAGACA CTATGGGTCA CAGCACAGGT TGTGGCAGAG AGAGACTCAT GGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839126.1
CDS	134..4030
Translation	MPDAGSHCPGAPAGLGPLLLALLLLLPPMVPAPRTSAQEVPDMMPPPGCSGGNCHPPTGNLVIGRGQSLRTSSTCGLHGPELYCVVSKLQALLQDSQNCCFCDSRDGKSHGIENVVSRSDPDGRKTWWQAESGVENVTIQLDLEGAFYFTHLIMTFKTFRPAALLLERSVDHGHSWHVFRYFAHNCSGLFPGIPPAPGRRVSDLVCDQRYSDIEPATEGEVIFQVLDPSFLAENTNNAEIQELLRVTNLRVNFSKLHTLGDRPLGGLRGHPFYYYALYELVIAGSCLCHGHASECRPAPGSPPSVEGMVHGHCVCRHHTTGTHCERCQGLYQDHPWQAAEPGYPHTCQECKCHGHARSCHFDMALYLASGNVSGGVCDACQHNTAGRHCELCQPFFHRDPLEDPRSLHSCKPCDCNPMGALEGGLCDAYTDTTRGLLSGQCRCKVHVWGQRCDSCRPGHYGLSLTQSEGCQPCRCNVRGRVPSTQACDPSSGACHCKRFVSGRDCSRCLPEFWGLSSDSLGCRPCDCDFGGAYSNRCSAGQGLCLCRPHLHGRHCQELQSGYFCATLDQATAEAEHGRSLQPADPQLPGAPWPAPRYCTPAPGTSSGWLGPRLWRQRDPDCVLQRARQARYSHQLPRTPGKPPPLHQGSGFALGRGSDFARVVDGAGLSLLAPTVPRALDYDIVLRYETQAPEVWQALVRVRAQSQPRSTRCARPLPSEQLFQATLTHARRAVVLSRPFCFEPGTRYSVTLRLWRTKEGRRPEGGTILLDSVVLLPRVGELPGLRSVDPGALRRLQELHEAGCLEAARVSPPQSMPEACARLACSISALLHGGGLACECHLQGSLSTECAPLGGQCPCRPNITGRTCDRCLPGTFGLGPTGCHECRCHPEGAASAVCNSTSGQCTCWAGCAGRRCDRCLSGRWGFPRCQPCACNGHAELCHPLTGVCQDCRGATTGRHCERCLDGYYGDPTLGSGQRCQPCPCPGHPGSGLYHGTSCHVDSTSGRVLCLCAPGYAGPRCDRCSPGYFGRPWPGDDPRRSPCRPCQCNNNIDPRDPAACDPHSGHCQRCLHHSHGAGCAHCRPGFHGSALRPGGCRRCSCDPRGTSPVRCPPEAEACFCDPVSGQCPCRPHTLGRDCSRCAPLFWNLGGPRGCEPCSCHARHTLQPGCHPVTGQCPCRAGFGGRTCSRCQDGYWGDPEQECRACACDPQGSISPSCDPHTGTCRCREGISGPRCQACARGSTGGFPHCTSCPPCFASWDQRLAPLQLHLDTMVHEDRKNHIGPDTMGHSTGCGRERLMG

Target ORF information:

RefSeq Version	XM_024983358.1
Organism	Bos taurus(cattle)
Definition	Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983358.1

ORF Insert Sequence:

ATGCCTGATG CTGGCTCCCA CTGCCCAGGA GCCCCAGCCG GGCTAGGCCC CCTTCTCTTA 
GCCCTGCTGC TGCTGCTGCC ACCCATGGTC CCGGCTCCCA GAACAAGTGC CCAGGAGGTA 
CCAGACATGA TGCCTCCTCC CGGCTGCTCA GGTGGCAACT GCCACCCACC TACTGGCAAC 
CTGGTTATTG GCCGTGGCCA GAGCCTTCGA ACCTCATCTA CCTGTGGCCT CCATGGCCCT 
GAACTCTACT GTGTTGTCAG TAAACTACAG GCGCTTCTCC AGGACTCTCA GAATTGCTGC 
TTTTGTGATT CTCGGGATGG GAAAAGCCAT GGCATTGAGA ATGTGGTCTC CCGGAGTGAC 
CCTGATGGCA GAAAGACCTG GTGGCAAGCA GAGAGTGGTG TTGAGAATGT CACCATCCAG 
TTGGACCTAG AAGGTGCCTT TTACTTTACC CACCTCATCA TGACTTTTAA GACATTCCGC 
CCAGCAGCTC TGCTCCTTGA GCGCTCAGTG GACCATGGCC ACTCCTGGCA TGTGTTCCGC 
TACTTTGCCC ACAACTGCTC AGGCCTCTTT CCTGGGATCC CTCCTGCCCC TGGCCGCAGA 
GTCAGTGACC TCGTCTGTGA CCAGCGTTAC TCAGACATCG AGCCTGCCAC TGAGGGAGAG 
GTAATTTTCC AAGTTCTGGA CCCATCCTTT CTGGCGGAGA ACACAAACAA CGCGGAGATT 
CAGGAACTCC TGCGTGTGAC TAACCTCCGT GTGAACTTCT CCAAGCTGCA CACACTGGGT 
GACAGGCCCC TGGGGGGCCT CAGGGGCCAC CCCTTCTACT ACTATGCTCT CTATGAGCTA 
GTGATTGCAG GCAGCTGCCT GTGCCACGGC CATGCTTCCG AGTGCAGGCC CGCACCCGGG 
TCCCCACCCA GCGTGGAAGG CATGGTGCAT GGCCATTGTG TCTGCCGCCA CCACACCACT 
GGTACCCACT GTGAACGTTG CCAGGGCCTG TACCAGGACC ACCCGTGGCA GGCAGCAGAG 
CCTGGGTACC CCCACACCTG CCAGGAATGC AAGTGCCATG GGCACGCCCG CAGTTGTCAC 
TTTGACATGG CCCTGTATCT CGCATCTGGC AACGTGAGTG GAGGTGTGTG CGATGCATGT 
CAACACAACA CGGCCGGGCG CCACTGTGAG CTCTGCCAGC CCTTCTTCCA CCGGGACCCC 
CTGGAGGACC CCCGCTCCCT TCACTCCTGC AAACCCTGTG ACTGCAACCC TATGGGTGCC 
CTGGAAGGGG GTTTGTGTGA TGCCTACACA GACACCACCC GGGGTCTCCT CTCTGGGCAG 
TGTCGCTGCA AAGTTCACGT CTGGGGCCAG CGCTGTGACT CCTGCCGGCC AGGTCACTAT 
GGCCTGAGCC TCACCCAGTC AGAAGGCTGC CAGCCCTGCA GGTGCAACGT CCGGGGCCGG 
GTCCCCAGCA CTCAGGCCTG TGATCCATCC AGCGGTGCCT GTCACTGCAA ACGCTTTGTC 
TCTGGACGGG ACTGCAGCCG CTGTCTGCCT GAGTTCTGGG GTCTGAGCAG TGACTCTCTA 
GGCTGTCGGC CCTGTGACTG TGACTTTGGG GGTGCCTACA GCAACAGGTG TTCTGCAGGG 
CAGGGCCTCT GCCTCTGCCG CCCCCACCTG CATGGCCGCC ACTGCCAGGA ACTCCAGTCT 
GGGTACTTCT GCGCCACCCT CGACCAGGCC ACTGCCGAAG CAGAGCATGG CCGCAGCCTC 
CAGCCTGCTG ACCCCCAGCT GCCTGGAGCC CCCTGGCCTG CTCCCCGCTA CTGTACACCA 
GCCCCAGGGA CCTCCTCTGG TTGGCTCGGG CCCCGACTTT GGAGACAGCG TGACCCTGAC 
TGTGTCCTCC AACGTGCCAG GCAAGCCCGG TACAGCCACC AGCTGCCCAG GACCCCAGGG 
AAGCCCCCGC CCCTGCATCA GGGATCTGGC TTTGCACTGG GGAGGGGCTC AGACTTCGCA 
CGTGTGGTGG ACGGGGCTGG CCTCTCTCTG CTGGCACCCA CAGTGCCTCG CGCCCTGGAC 
TATGACATCG TCCTACGCTA TGAGACCCAG GCACCTGAAG TGTGGCAGGC ATTAGTCCGG 
GTCCGTGCCC AGTCACAGCC CCGCAGTACC CGCTGTGCCC GTCCGTTGCC CTCAGAGCAG 
CTGTTCCAGG CGACCTTGAC CCATGCGCGC AGAGCCGTGG TTCTTTCCAG ACCGTTCTGC 
TTTGAACCCG GAACCCGCTA CTCCGTGACC CTGCGGCTGT GGCGGACCAA GGAGGGACGG 
AGACCCGAAG GGGGCACGAT CCTCCTGGAT TCAGTAGTGC TCCTGCCGCG GGTTGGAGAG 
TTGCCTGGAC TGAGGTCCGT GGACCCGGGG GCCCTGAGGC GCTTGCAGGA GCTCCACGAG 
GCGGGCTGCC TGGAGGCAGC GAGGGTGAGC CCACCCCAAA GCATGCCTGA GGCCTGTGCC 
CGCCTCGCCT GCAGCATCTC AGCCCTGCTC CATGGAGGTG GCCTCGCATG TGAGTGCCAC 
CTGCAGGGTT CACTGAGCAC CGAGTGTGCC CCACTGGGGG GGCAATGCCC CTGCCGCCCT 
AATATCACAG GTCGTACCTG TGACCGCTGC TTGCCTGGGA CATTTGGCCT CGGGCCCACC 
GGGTGCCATG AGTGCCGCTG CCACCCCGAG GGGGCTGCCA GTGCCGTCTG TAACTCTACG 
AGTGGGCAGT GCACGTGTTG GGCAGGCTGT GCTGGTCGCC GCTGTGACCG TTGCCTCTCT 
GGCCGGTGGG GCTTCCCACG CTGCCAGCCC TGTGCCTGCA ATGGACACGC CGAGTTGTGC 
CACCCACTCA CAGGCGTCTG CCAGGACTGC CGCGGAGCCA CCACAGGCCG GCATTGTGAG 
AGGTGCTTGG ATGGCTACTA TGGAGACCCC ACCCTGGGCT CAGGCCAGCG GTGCCAGCCC 
TGCCCCTGTC CTGGGCACCC TGGCTCAGGC CTCTATCATG GGACCTCCTG CCATGTGGAC 
AGCACCAGTG GACGTGTCCT GTGCCTCTGT GCCCCCGGCT ATGCAGGACC CCGCTGTGAC 
CGCTGCTCCC CCGGCTACTT TGGGCGCCCC TGGCCTGGAG ATGACCCCAG AAGGAGTCCC 
TGCCGGCCCT GCCAGTGCAA CAACAATATT GATCCCCGTG ACCCAGCCGC CTGTGACCCC 
CACAGCGGAC ATTGCCAGCG CTGTCTGCAC CACAGCCACG GCGCTGGCTG TGCCCACTGC 
CGGCCTGGCT TCCACGGCAG TGCCCTGCGC CCAGGGGGCT GCCGGCGCTG CAGCTGTGAC 
CCCCGGGGCA CCAGCCCTGT AAGGTGCCCA CCTGAGGCTG AAGCCTGCTT CTGTGACCCG 
GTCAGCGGGC AGTGCCCCTG CCGCCCCCAC ACACTGGGGC GGGACTGTAG CCGCTGTGCC 
CCCCTCTTCT GGAACCTCGG GGGGCCCCGG GGCTGTGAGC CCTGCAGCTG CCACGCCCGA 
CACACTTTGC AGCCGGGGTG TCACCCGGTC ACGGGTCAGT GCCCCTGCCG GGCAGGATTT 
GGGGGCCGCA CGTGCTCCCG GTGTCAGGAT GGGTACTGGG GTGACCCGGA GCAGGAGTGC 
AGAGCCTGTG CCTGTGACCC CCAGGGCTCC ATCTCGCCCA GCTGTGACCC GCACACAGGC 
ACCTGCCGCT GCCGAGAGGG TATCTCAGGG CCGAGGTGCC AGGCGTGTGC CCGTGGCTCC 
ACAGGAGGCT TCCCACACTG CACGTCCTGC CCTCCCTGCT TCGCCTCCTG GGACCAACGC 
CTGGCTCCCC TCCAGCTGCA TCTGGACACC ATGGTCCATG AGGACAGAAA GAACCACATT 
GGGCCAGACA CTATGGGTCA CAGCACAGGT TGTGGCAGAG AGAGACTCAT GGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC530599 ( XM_015468730.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324216.1 Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa94461
Clone ID Related Accession (Same CDS sequence)	XM_015468730.1 , XM_015459647.1
Accession Version	XM_015468730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	laminin subunit beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATG CTGGCTCCCA CTGCCCAGGA GCCCCAGCCG GGCTAGGCCC CCTTCTCTTA 
GCCCTGCTGC TGCTGCTGCC ACCCATGGTC CCGGCTCCCA GAACAAGTGC CCAGGAGGTA 
CCAGACATGA TGCCTCCTCC CGGCTGCTCA GGTGGCAACT GCCACCCACC TACTGGCAAC 
CTGGTTATTG GCCGTGGCCA GAGCCTTCGA ACCTCATCTA CCTGTGGCCT CCATGGCCCT 
GAACTCTACT GTGTTGTCAG TAAACTACAG GCGCTTCTCC AGGACTCTCA GAATTGCTGC 
TTTTGTGATT CTCGGGATGG GAAAAGCCAT GGCATTGAGA ATGTGGTCTC CCGGAGTGAC 
CCTGATGGCA GAAAGACCTG GTGGCAAGCA GAGAGTGGTG TTGAGAATGT CACCATCCAG 
TTGGACCTAG AAGGTGCCTT TTACTTTACC CACCTCATCA TGACTTTTAA GACATTCCGC 
CCAGCAGCTC TGCTCCTTGA GCGCTCAGTG GACCATGGCC ACTCCTGGCA TGTGTTCCGC 
TACTTTGCCC ACAACTGCTC AGGCCTCTTT CCTGGGATCC CTCCTGCCCC TGGCCGCAGA 
GTCAGTGACC TCGTCTGTGA CCAGCGTTAC TCAGACATCG AGCCTGCCAC TGAGGGAGAG 
GTAATTTTCC AAGTTCTGGA CCCATCCTTT CTGGTGGAGA ACACAAACAA CGCGGAGATT 
CAGGAACTCC TGCGTGTGAC TAACCTCCGT GTGAACTTCT CCAAGCTGCA CACACTGGGT 
GACAGGCCCC TGGGGGGCCT CAGGGGCCAC CCCTTCTACT ACTATGCTCT CTATGAGCTA 
GTGATTGCAG GCAGCTGCCT GTGCCACGGC CATGCTTCCG AGTGCAGGCC CGCACCCGGG 
TCCCCACCCA GCGTGGAAGG CATGGTGCAT GGCCATTGTG TCTGCCGCCA CCACACCACT 
GGTACCCACT GTGAACGTTG CCAGGGCCTG TACCAGGACC ACCCGTGGCA GGCAGCAGAG 
CCTGGGTACC CCCACACCTG CCAGGAATGC AAGTGCCATG GGCACGCCCG CAGTTGTCAC 
TTTGACATGG CCCTGTATCT CGCATCTGGC AACGTGAGTG GAGGTGTGTG CGATGCATGT 
CAACACAACA CGGCCGGGCG CCACTGTGAG CTCTGCCAGC CCTTCTTCCA CCGGGACCCC 
CTGGAGGACC CCCGCTCCCT TCACTCCTGC AAACCCTGTG ACTGCAACCC TATGGGTGCC 
CTGGAAGGGG GTTTGTGTGA TGCCTACACA GACACCACCC GGGGTCTCCT CTCTGGGCAG 
TGTCGCTGCA AAGTTCACGT CTGGGGCCAG CGCTGTGACT CCTGCCGGCC AGGTCACTAT 
GGCCTGAGCC TCACCCAGTC AGAAGGCTGC CAGCCCTGCA GGTGCAACGC CCGGGGCCGG 
GTCCCCAGCA CTCAGGCCTG TGATCCATCC AGCGGTGCCT GTCACTGCAA ACGCTTTGTC 
TCTGGACGGG ACTGCAGCCG CTGTCTGCCT GAGTTCTGGG GTCTGAGCAG TGACTCTCTA 
GGCTGTCGGC CCTGTGACTG TGACTTTGGG GGTGCCTACA GCAACAGGTG TTCTGCAGGG 
CAGGGCCTCT GCCTCTGCCG CCCCCACCTG CATGGCCGCC ACTGCCAGGA ACTCCAGTCT 
GGGTACTTCT GCGCCACCCT CGACCAGGCC ACTGCCGAAG CAGAGCATGG CCGCAGCCTC 
CAGCCTGCTG ACCCCCAGCT GCCTGGAGCC CCCTGGCCTG CTCCCCGCTA CTGTACACCA 
GCCCCAGGGA CCTCCTCTGG TTGGCTCGGG CCCCGACTTT GGAGACAGCG TGACCCTGAC 
TGTGTCCTCC AACGTGCCAG GCAAGCCCGG TACAGCCACC AGCTGCCCAG GACCCCAGGG 
AAGCCCCCGC CCCTGCATCA GGGATCTGGC TTTGCACTGG GGAGGGGCTC AGACTTCGCA 
CGTGTGGTGG ACGGGGCTGG CCTCTCTCTG CTGGCACCCA CAGTGCCTCG CGCCCTGGAC 
TATGACATCG TCCTACGCTA TGAGACCCAG GCACCTGAAG TGTGGCAGGC ATTAGTCCGG 
GTCCGTGCCC AGTCACAGCC CCGCAGTACC CGCTGTGCCC GTCCGTTGCC CTCAGAGCAG 
CTGTTCCAGG CGACCTTGAC CCATGCGCGC AGAGCCGTGG TTCTTTCCAG ACCGTTCTGC 
TTTGAACCCG GAACCCGCTA CTCCGTGACC CTGCGGCTGT GGCGGACCAA GGAGGGACGG 
AGACCCGAAG GGGGCACGAT CCTCCTGGAT TCAGTAGTGC TCCTGCCGCG GGTTGGAGAG 
TTGCCTGGAC TGAGGTCCGT GGACCCGGGG GCCCTGAGGC GCTTGCAGGA GCTCCACGAG 
GCGGGCTGCC TGGAGGCAGC GAGGGTGAGC CCACCCCAAA GCATGCCTGA GGCCTGTGCC 
CGCCTCGCCT GCAGCATCTC AGCCCTGCTC CATGGAGGTG GCCTCGCATG TGAGTGCCAC 
CTGCAGGGTT CACTGAGCAC CGAGTGTGCC CCACTGGGGG GGCAATGCCC CTGCCGCCCT 
AATATCACAG GTCGTACCTG TGACCGCTGC TTGCCTGGGA CATTTGGCCT CGGGCCCACC 
GGGTGCCATG AGTGCCGCTG CCACCCCGAG GGGGCTGCCA GTGCCGTCTG TAACTCTACG 
AGTGGGCAGT GCACGTGTTG GGCAGGCTGT GCTGGTCGCC GCTGTGACCG TTGCCTCTCT 
GGCCGGTGGG GCTTCCCACG CTGCCAGCCC TGTGCCTGCA ATGGACACGC CGAGTTGTGC 
CACCCACTCA CAGGCGTCTG CCAGGACTGC CGCGGAGCCA CCACAGGCCG GCATTGTGAG 
AGGTGCTTGG ATGGCTACTA TGGAGACCCC ACCCTGGGCT CAGGCCAGCG GTGCCAGCCC 
TGCCCCTGTC CTGGGCACCC TGGCTCAGGC CTCTATCATG GGACCTCCTG CCATGTGGAC 
AGCACCAGTG GACGTGTCCT GTGCCTCTGT GCCCCCGGCT ATGCAGGACC CCGCTGTGAC 
CGCTGCTCCC CCGGCTACTT TGGGCGCCCC TGGCCTGGAG ATGACCCCAG AAGGAGTCCC 
TGCCGGCCCT GCCAGTGCAA CAACAATATT GATCCCCGTG ACCCAGCCGC CTGTGACCCC 
CACAGCGGAC ATTGCCAGCG CTGTCTGCAC CACAGCCACG GCGCTGGCTG TGCCCACTGC 
CGGCCTGGCT TCCACGGCAG TGCCCTGCGC CCAGGGGGCT GCCGGCGCTG CAGCTGTGAC 
CCCCGGGGCA CCAGCCCTGT AAGGTGCCCA CCTGAGGCTG AAGCCTGCTT CTGTGACCCG 
GTCAGCGGGC AGTGCCCCTG CCGCCCCCAC ACACTGGGGC GGGACTGTAG CCGCTGTGCC 
CCCCTCTTCT GGAACCTCGG GGGGCCCCGG GGCTGTGAGC CCTGCAGCTG CCACGCCCGA 
CACACTTTGC AGCCGGGGTG TCACCCGGTC ACGGGTCAGT GCCCCTGCCG GGCAGGATTT 
GGGGGCCGCA CGTGCTCCCG GTGTCAGGAT GGGTACTGGG GTGACCCGGA GCAGGAGTGC 
AGAGCCTGTG CCTGTGACCC CCAGGGCTCC ATCTCGCCCA GCTGTGACCC GCACACAGGC 
ACCTGCCGCT GCCGAGAGGG TATCTCAGGG CCGAGGTGCC AGGCGTGTGC CCGTGGCTCC 
ACAGGAGGCT TCCCACACTG CACGTCCTGC CCTCCCTGCT TCGCCTCCTG GGACCAACGC 
CTGGCTCCCC TCCAGCTGCA TCTGGACACC ATGGTCCATG AGGTGGCTGC CCTGCGCCAG 
GGCATGCCTG GCTGGGGTGC TGGGCTCCGG GGAGATCAGC TGCAGGCCCT GGAGGGTAAA 
CTCCAGCAGG CTCAGACACT CCTGGAATCA CCTTCCCCCA CCAGAGGTCC CCTGCAACAA 
TTTACAGAGT GGATCACCGG ACTCAGGCAA GGGGTCTGGG GTCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324216.1
CDS	1033..5040
Translation	MPDAGSHCPGAPAGLGPLLLALLLLLPPMVPAPRTSAQEVPDMMPPPGCSGGNCHPPTGNLVIGRGQSLRTSSTCGLHGPELYCVVSKLQALLQDSQNCCFCDSRDGKSHGIENVVSRSDPDGRKTWWQAESGVENVTIQLDLEGAFYFTHLIMTFKTFRPAALLLERSVDHGHSWHVFRYFAHNCSGLFPGIPPAPGRRVSDLVCDQRYSDIEPATEGEVIFQVLDPSFLVENTNNAEIQELLRVTNLRVNFSKLHTLGDRPLGGLRGHPFYYYALYELVIAGSCLCHGHASECRPAPGSPPSVEGMVHGHCVCRHHTTGTHCERCQGLYQDHPWQAAEPGYPHTCQECKCHGHARSCHFDMALYLASGNVSGGVCDACQHNTAGRHCELCQPFFHRDPLEDPRSLHSCKPCDCNPMGALEGGLCDAYTDTTRGLLSGQCRCKVHVWGQRCDSCRPGHYGLSLTQSEGCQPCRCNARGRVPSTQACDPSSGACHCKRFVSGRDCSRCLPEFWGLSSDSLGCRPCDCDFGGAYSNRCSAGQGLCLCRPHLHGRHCQELQSGYFCATLDQATAEAEHGRSLQPADPQLPGAPWPAPRYCTPAPGTSSGWLGPRLWRQRDPDCVLQRARQARYSHQLPRTPGKPPPLHQGSGFALGRGSDFARVVDGAGLSLLAPTVPRALDYDIVLRYETQAPEVWQALVRVRAQSQPRSTRCARPLPSEQLFQATLTHARRAVVLSRPFCFEPGTRYSVTLRLWRTKEGRRPEGGTILLDSVVLLPRVGELPGLRSVDPGALRRLQELHEAGCLEAARVSPPQSMPEACARLACSISALLHGGGLACECHLQGSLSTECAPLGGQCPCRPNITGRTCDRCLPGTFGLGPTGCHECRCHPEGAASAVCNSTSGQCTCWAGCAGRRCDRCLSGRWGFPRCQPCACNGHAELCHPLTGVCQDCRGATTGRHCERCLDGYYGDPTLGSGQRCQPCPCPGHPGSGLYHGTSCHVDSTSGRVLCLCAPGYAGPRCDRCSPGYFGRPWPGDDPRRSPCRPCQCNNNIDPRDPAACDPHSGHCQRCLHHSHGAGCAHCRPGFHGSALRPGGCRRCSCDPRGTSPVRCPPEAEACFCDPVSGQCPCRPHTLGRDCSRCAPLFWNLGGPRGCEPCSCHARHTLQPGCHPVTGQCPCRAGFGGRTCSRCQDGYWGDPEQECRACACDPQGSISPSCDPHTGTCRCREGISGPRCQACARGSTGGFPHCTSCPPCFASWDQRLAPLQLHLDTMVHEVAALRQGMPGWGAGLRGDQLQALEGKLQQAQTLLESPSPTRGPLQQFTEWITGLRQGVWGH

Target ORF information:

RefSeq Version	XM_015468730.1
Organism	Bos taurus(cattle)
Definition	Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468730.1

ORF Insert Sequence:

ATGCCTGATG CTGGCTCCCA CTGCCCAGGA GCCCCAGCCG GGCTAGGCCC CCTTCTCTTA 
GCCCTGCTGC TGCTGCTGCC ACCCATGGTC CCGGCTCCCA GAACAAGTGC CCAGGAGGTA 
CCAGACATGA TGCCTCCTCC CGGCTGCTCA GGTGGCAACT GCCACCCACC TACTGGCAAC 
CTGGTTATTG GCCGTGGCCA GAGCCTTCGA ACCTCATCTA CCTGTGGCCT CCATGGCCCT 
GAACTCTACT GTGTTGTCAG TAAACTACAG GCGCTTCTCC AGGACTCTCA GAATTGCTGC 
TTTTGTGATT CTCGGGATGG GAAAAGCCAT GGCATTGAGA ATGTGGTCTC CCGGAGTGAC 
CCTGATGGCA GAAAGACCTG GTGGCAAGCA GAGAGTGGTG TTGAGAATGT CACCATCCAG 
TTGGACCTAG AAGGTGCCTT TTACTTTACC CACCTCATCA TGACTTTTAA GACATTCCGC 
CCAGCAGCTC TGCTCCTTGA GCGCTCAGTG GACCATGGCC ACTCCTGGCA TGTGTTCCGC 
TACTTTGCCC ACAACTGCTC AGGCCTCTTT CCTGGGATCC CTCCTGCCCC TGGCCGCAGA 
GTCAGTGACC TCGTCTGTGA CCAGCGTTAC TCAGACATCG AGCCTGCCAC TGAGGGAGAG 
GTAATTTTCC AAGTTCTGGA CCCATCCTTT CTGGTGGAGA ACACAAACAA CGCGGAGATT 
CAGGAACTCC TGCGTGTGAC TAACCTCCGT GTGAACTTCT CCAAGCTGCA CACACTGGGT 
GACAGGCCCC TGGGGGGCCT CAGGGGCCAC CCCTTCTACT ACTATGCTCT CTATGAGCTA 
GTGATTGCAG GCAGCTGCCT GTGCCACGGC CATGCTTCCG AGTGCAGGCC CGCACCCGGG 
TCCCCACCCA GCGTGGAAGG CATGGTGCAT GGCCATTGTG TCTGCCGCCA CCACACCACT 
GGTACCCACT GTGAACGTTG CCAGGGCCTG TACCAGGACC ACCCGTGGCA GGCAGCAGAG 
CCTGGGTACC CCCACACCTG CCAGGAATGC AAGTGCCATG GGCACGCCCG CAGTTGTCAC 
TTTGACATGG CCCTGTATCT CGCATCTGGC AACGTGAGTG GAGGTGTGTG CGATGCATGT 
CAACACAACA CGGCCGGGCG CCACTGTGAG CTCTGCCAGC CCTTCTTCCA CCGGGACCCC 
CTGGAGGACC CCCGCTCCCT TCACTCCTGC AAACCCTGTG ACTGCAACCC TATGGGTGCC 
CTGGAAGGGG GTTTGTGTGA TGCCTACACA GACACCACCC GGGGTCTCCT CTCTGGGCAG 
TGTCGCTGCA AAGTTCACGT CTGGGGCCAG CGCTGTGACT CCTGCCGGCC AGGTCACTAT 
GGCCTGAGCC TCACCCAGTC AGAAGGCTGC CAGCCCTGCA GGTGCAACGC CCGGGGCCGG 
GTCCCCAGCA CTCAGGCCTG TGATCCATCC AGCGGTGCCT GTCACTGCAA ACGCTTTGTC 
TCTGGACGGG ACTGCAGCCG CTGTCTGCCT GAGTTCTGGG GTCTGAGCAG TGACTCTCTA 
GGCTGTCGGC CCTGTGACTG TGACTTTGGG GGTGCCTACA GCAACAGGTG TTCTGCAGGG 
CAGGGCCTCT GCCTCTGCCG CCCCCACCTG CATGGCCGCC ACTGCCAGGA ACTCCAGTCT 
GGGTACTTCT GCGCCACCCT CGACCAGGCC ACTGCCGAAG CAGAGCATGG CCGCAGCCTC 
CAGCCTGCTG ACCCCCAGCT GCCTGGAGCC CCCTGGCCTG CTCCCCGCTA CTGTACACCA 
GCCCCAGGGA CCTCCTCTGG TTGGCTCGGG CCCCGACTTT GGAGACAGCG TGACCCTGAC 
TGTGTCCTCC AACGTGCCAG GCAAGCCCGG TACAGCCACC AGCTGCCCAG GACCCCAGGG 
AAGCCCCCGC CCCTGCATCA GGGATCTGGC TTTGCACTGG GGAGGGGCTC AGACTTCGCA 
CGTGTGGTGG ACGGGGCTGG CCTCTCTCTG CTGGCACCCA CAGTGCCTCG CGCCCTGGAC 
TATGACATCG TCCTACGCTA TGAGACCCAG GCACCTGAAG TGTGGCAGGC ATTAGTCCGG 
GTCCGTGCCC AGTCACAGCC CCGCAGTACC CGCTGTGCCC GTCCGTTGCC CTCAGAGCAG 
CTGTTCCAGG CGACCTTGAC CCATGCGCGC AGAGCCGTGG TTCTTTCCAG ACCGTTCTGC 
TTTGAACCCG GAACCCGCTA CTCCGTGACC CTGCGGCTGT GGCGGACCAA GGAGGGACGG 
AGACCCGAAG GGGGCACGAT CCTCCTGGAT TCAGTAGTGC TCCTGCCGCG GGTTGGAGAG 
TTGCCTGGAC TGAGGTCCGT GGACCCGGGG GCCCTGAGGC GCTTGCAGGA GCTCCACGAG 
GCGGGCTGCC TGGAGGCAGC GAGGGTGAGC CCACCCCAAA GCATGCCTGA GGCCTGTGCC 
CGCCTCGCCT GCAGCATCTC AGCCCTGCTC CATGGAGGTG GCCTCGCATG TGAGTGCCAC 
CTGCAGGGTT CACTGAGCAC CGAGTGTGCC CCACTGGGGG GGCAATGCCC CTGCCGCCCT 
AATATCACAG GTCGTACCTG TGACCGCTGC TTGCCTGGGA CATTTGGCCT CGGGCCCACC 
GGGTGCCATG AGTGCCGCTG CCACCCCGAG GGGGCTGCCA GTGCCGTCTG TAACTCTACG 
AGTGGGCAGT GCACGTGTTG GGCAGGCTGT GCTGGTCGCC GCTGTGACCG TTGCCTCTCT 
GGCCGGTGGG GCTTCCCACG CTGCCAGCCC TGTGCCTGCA ATGGACACGC CGAGTTGTGC 
CACCCACTCA CAGGCGTCTG CCAGGACTGC CGCGGAGCCA CCACAGGCCG GCATTGTGAG 
AGGTGCTTGG ATGGCTACTA TGGAGACCCC ACCCTGGGCT CAGGCCAGCG GTGCCAGCCC 
TGCCCCTGTC CTGGGCACCC TGGCTCAGGC CTCTATCATG GGACCTCCTG CCATGTGGAC 
AGCACCAGTG GACGTGTCCT GTGCCTCTGT GCCCCCGGCT ATGCAGGACC CCGCTGTGAC 
CGCTGCTCCC CCGGCTACTT TGGGCGCCCC TGGCCTGGAG ATGACCCCAG AAGGAGTCCC 
TGCCGGCCCT GCCAGTGCAA CAACAATATT GATCCCCGTG ACCCAGCCGC CTGTGACCCC 
CACAGCGGAC ATTGCCAGCG CTGTCTGCAC CACAGCCACG GCGCTGGCTG TGCCCACTGC 
CGGCCTGGCT TCCACGGCAG TGCCCTGCGC CCAGGGGGCT GCCGGCGCTG CAGCTGTGAC 
CCCCGGGGCA CCAGCCCTGT AAGGTGCCCA CCTGAGGCTG AAGCCTGCTT CTGTGACCCG 
GTCAGCGGGC AGTGCCCCTG CCGCCCCCAC ACACTGGGGC GGGACTGTAG CCGCTGTGCC 
CCCCTCTTCT GGAACCTCGG GGGGCCCCGG GGCTGTGAGC CCTGCAGCTG CCACGCCCGA 
CACACTTTGC AGCCGGGGTG TCACCCGGTC ACGGGTCAGT GCCCCTGCCG GGCAGGATTT 
GGGGGCCGCA CGTGCTCCCG GTGTCAGGAT GGGTACTGGG GTGACCCGGA GCAGGAGTGC 
AGAGCCTGTG CCTGTGACCC CCAGGGCTCC ATCTCGCCCA GCTGTGACCC GCACACAGGC 
ACCTGCCGCT GCCGAGAGGG TATCTCAGGG CCGAGGTGCC AGGCGTGTGC CCGTGGCTCC 
ACAGGAGGCT TCCCACACTG CACGTCCTGC CCTCCCTGCT TCGCCTCCTG GGACCAACGC 
CTGGCTCCCC TCCAGCTGCA TCTGGACACC ATGGTCCATG AGGTGGCTGC CCTGCGCCAG 
GGCATGCCTG GCTGGGGTGC TGGGCTCCGG GGAGATCAGC TGCAGGCCCT GGAGGGTAAA 
CTCCAGCAGG CTCAGACACT CCTGGAATCA CCTTCCCCCA CCAGAGGTCC CCTGCAACAA 
TTTACAGAGT GGATCACCGG ACTCAGGCAA GGGGTCTGGG GTCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC530599 ( XM_015459647.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315133.1 Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa94461
Clone ID Related Accession (Same CDS sequence)	XM_015468730.1 , XM_015459647.1
Accession Version	XM_015459647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	laminin subunit beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATG CTGGCTCCCA CTGCCCAGGA GCCCCAGCCG GGCTAGGCCC CCTTCTCTTA 
GCCCTGCTGC TGCTGCTGCC ACCCATGGTC CCGGCTCCCA GAACAAGTGC CCAGGAGGTA 
CCAGACATGA TGCCTCCTCC CGGCTGCTCA GGTGGCAACT GCCACCCACC TACTGGCAAC 
CTGGTTATTG GCCGTGGCCA GAGCCTTCGA ACCTCATCTA CCTGTGGCCT CCATGGCCCT 
GAACTCTACT GTGTTGTCAG TAAACTACAG GCGCTTCTCC AGGACTCTCA GAATTGCTGC 
TTTTGTGATT CTCGGGATGG GAAAAGCCAT GGCATTGAGA ATGTGGTCTC CCGGAGTGAC 
CCTGATGGCA GAAAGACCTG GTGGCAAGCA GAGAGTGGTG TTGAGAATGT CACCATCCAG 
TTGGACCTAG AAGGTGCCTT TTACTTTACC CACCTCATCA TGACTTTTAA GACATTCCGC 
CCAGCAGCTC TGCTCCTTGA GCGCTCAGTG GACCATGGCC ACTCCTGGCA TGTGTTCCGC 
TACTTTGCCC ACAACTGCTC AGGCCTCTTT CCTGGGATCC CTCCTGCCCC TGGCCGCAGA 
GTCAGTGACC TCGTCTGTGA CCAGCGTTAC TCAGACATCG AGCCTGCCAC TGAGGGAGAG 
GTAATTTTCC AAGTTCTGGA CCCATCCTTT CTGGTGGAGA ACACAAACAA CGCGGAGATT 
CAGGAACTCC TGCGTGTGAC TAACCTCCGT GTGAACTTCT CCAAGCTGCA CACACTGGGT 
GACAGGCCCC TGGGGGGCCT CAGGGGCCAC CCCTTCTACT ACTATGCTCT CTATGAGCTA 
GTGATTGCAG GCAGCTGCCT GTGCCACGGC CATGCTTCCG AGTGCAGGCC CGCACCCGGG 
TCCCCACCCA GCGTGGAAGG CATGGTGCAT GGCCATTGTG TCTGCCGCCA CCACACCACT 
GGTACCCACT GTGAACGTTG CCAGGGCCTG TACCAGGACC ACCCGTGGCA GGCAGCAGAG 
CCTGGGTACC CCCACACCTG CCAGGAATGC AAGTGCCATG GGCACGCCCG CAGTTGTCAC 
TTTGACATGG CCCTGTATCT CGCATCTGGC AACGTGAGTG GAGGTGTGTG CGATGCATGT 
CAACACAACA CGGCCGGGCG CCACTGTGAG CTCTGCCAGC CCTTCTTCCA CCGGGACCCC 
CTGGAGGACC CCCGCTCCCT TCACTCCTGC AAACCCTGTG ACTGCAACCC TATGGGTGCC 
CTGGAAGGGG GTTTGTGTGA TGCCTACACA GACACCACCC GGGGTCTCCT CTCTGGGCAG 
TGTCGCTGCA AAGTTCACGT CTGGGGCCAG CGCTGTGACT CCTGCCGGCC AGGTCACTAT 
GGCCTGAGCC TCACCCAGTC AGAAGGCTGC CAGCCCTGCA GGTGCAACGC CCGGGGCCGG 
GTCCCCAGCA CTCAGGCCTG TGATCCATCC AGCGGTGCCT GTCACTGCAA ACGCTTTGTC 
TCTGGACGGG ACTGCAGCCG CTGTCTGCCT GAGTTCTGGG GTCTGAGCAG TGACTCTCTA 
GGCTGTCGGC CCTGTGACTG TGACTTTGGG GGTGCCTACA GCAACAGGTG TTCTGCAGGG 
CAGGGCCTCT GCCTCTGCCG CCCCCACCTG CATGGCCGCC ACTGCCAGGA ACTCCAGTCT 
GGGTACTTCT GCGCCACCCT CGACCAGGCC ACTGCCGAAG CAGAGCATGG CCGCAGCCTC 
CAGCCTGCTG ACCCCCAGCT GCCTGGAGCC CCCTGGCCTG CTCCCCGCTA CTGTACACCA 
GCCCCAGGGA CCTCCTCTGG TTGGCTCGGG CCCCGACTTT GGAGACAGCG TGACCCTGAC 
TGTGTCCTCC AACGTGCCAG GCAAGCCCGG TACAGCCACC AGCTGCCCAG GACCCCAGGG 
AAGCCCCCGC CCCTGCATCA GGGATCTGGC TTTGCACTGG GGAGGGGCTC AGACTTCGCA 
CGTGTGGTGG ACGGGGCTGG CCTCTCTCTG CTGGCACCCA CAGTGCCTCG CGCCCTGGAC 
TATGACATCG TCCTACGCTA TGAGACCCAG GCACCTGAAG TGTGGCAGGC ATTAGTCCGG 
GTCCGTGCCC AGTCACAGCC CCGCAGTACC CGCTGTGCCC GTCCGTTGCC CTCAGAGCAG 
CTGTTCCAGG CGACCTTGAC CCATGCGCGC AGAGCCGTGG TTCTTTCCAG ACCGTTCTGC 
TTTGAACCCG GAACCCGCTA CTCCGTGACC CTGCGGCTGT GGCGGACCAA GGAGGGACGG 
AGACCCGAAG GGGGCACGAT CCTCCTGGAT TCAGTAGTGC TCCTGCCGCG GGTTGGAGAG 
TTGCCTGGAC TGAGGTCCGT GGACCCGGGG GCCCTGAGGC GCTTGCAGGA GCTCCACGAG 
GCGGGCTGCC TGGAGGCAGC GAGGGTGAGC CCACCCCAAA GCATGCCTGA GGCCTGTGCC 
CGCCTCGCCT GCAGCATCTC AGCCCTGCTC CATGGAGGTG GCCTCGCATG TGAGTGCCAC 
CTGCAGGGTT CACTGAGCAC CGAGTGTGCC CCACTGGGGG GGCAATGCCC CTGCCGCCCT 
AATATCACAG GTCGTACCTG TGACCGCTGC TTGCCTGGGA CATTTGGCCT CGGGCCCACC 
GGGTGCCATG AGTGCCGCTG CCACCCCGAG GGGGCTGCCA GTGCCGTCTG TAACTCTACG 
AGTGGGCAGT GCACGTGTTG GGCAGGCTGT GCTGGTCGCC GCTGTGACCG TTGCCTCTCT 
GGCCGGTGGG GCTTCCCACG CTGCCAGCCC TGTGCCTGCA ATGGACACGC CGAGTTGTGC 
CACCCACTCA CAGGCGTCTG CCAGGACTGC CGCGGAGCCA CCACAGGCCG GCATTGTGAG 
AGGTGCTTGG ATGGCTACTA TGGAGACCCC ACCCTGGGCT CAGGCCAGCG GTGCCAGCCC 
TGCCCCTGTC CTGGGCACCC TGGCTCAGGC CTCTATCATG GGACCTCCTG CCATGTGGAC 
AGCACCAGTG GACGTGTCCT GTGCCTCTGT GCCCCCGGCT ATGCAGGACC CCGCTGTGAC 
CGCTGCTCCC CCGGCTACTT TGGGCGCCCC TGGCCTGGAG ATGACCCCAG AAGGAGTCCC 
TGCCGGCCCT GCCAGTGCAA CAACAATATT GATCCCCGTG ACCCAGCCGC CTGTGACCCC 
CACAGCGGAC ATTGCCAGCG CTGTCTGCAC CACAGCCACG GCGCTGGCTG TGCCCACTGC 
CGGCCTGGCT TCCACGGCAG TGCCCTGCGC CCAGGGGGCT GCCGGCGCTG CAGCTGTGAC 
CCCCGGGGCA CCAGCCCTGT AAGGTGCCCA CCTGAGGCTG AAGCCTGCTT CTGTGACCCG 
GTCAGCGGGC AGTGCCCCTG CCGCCCCCAC ACACTGGGGC GGGACTGTAG CCGCTGTGCC 
CCCCTCTTCT GGAACCTCGG GGGGCCCCGG GGCTGTGAGC CCTGCAGCTG CCACGCCCGA 
CACACTTTGC AGCCGGGGTG TCACCCGGTC ACGGGTCAGT GCCCCTGCCG GGCAGGATTT 
GGGGGCCGCA CGTGCTCCCG GTGTCAGGAT GGGTACTGGG GTGACCCGGA GCAGGAGTGC 
AGAGCCTGTG CCTGTGACCC CCAGGGCTCC ATCTCGCCCA GCTGTGACCC GCACACAGGC 
ACCTGCCGCT GCCGAGAGGG TATCTCAGGG CCGAGGTGCC AGGCGTGTGC CCGTGGCTCC 
ACAGGAGGCT TCCCACACTG CACGTCCTGC CCTCCCTGCT TCGCCTCCTG GGACCAACGC 
CTGGCTCCCC TCCAGCTGCA TCTGGACACC ATGGTCCATG AGGTGGCTGC CCTGCGCCAG 
GGCATGCCTG GCTGGGGTGC TGGGCTCCGG GGAGATCAGC TGCAGGCCCT GGAGGGTAAA 
CTCCAGCAGG CTCAGACACT CCTGGAATCA CCTTCCCCCA CCAGAGGTCC CCTGCAACAA 
TTTACAGAGT GGATCACCGG ACTCAGGCAA GGGGTCTGGG GTCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315133.1
CDS	1033..5040
Translation	MPDAGSHCPGAPAGLGPLLLALLLLLPPMVPAPRTSAQEVPDMMPPPGCSGGNCHPPTGNLVIGRGQSLRTSSTCGLHGPELYCVVSKLQALLQDSQNCCFCDSRDGKSHGIENVVSRSDPDGRKTWWQAESGVENVTIQLDLEGAFYFTHLIMTFKTFRPAALLLERSVDHGHSWHVFRYFAHNCSGLFPGIPPAPGRRVSDLVCDQRYSDIEPATEGEVIFQVLDPSFLVENTNNAEIQELLRVTNLRVNFSKLHTLGDRPLGGLRGHPFYYYALYELVIAGSCLCHGHASECRPAPGSPPSVEGMVHGHCVCRHHTTGTHCERCQGLYQDHPWQAAEPGYPHTCQECKCHGHARSCHFDMALYLASGNVSGGVCDACQHNTAGRHCELCQPFFHRDPLEDPRSLHSCKPCDCNPMGALEGGLCDAYTDTTRGLLSGQCRCKVHVWGQRCDSCRPGHYGLSLTQSEGCQPCRCNARGRVPSTQACDPSSGACHCKRFVSGRDCSRCLPEFWGLSSDSLGCRPCDCDFGGAYSNRCSAGQGLCLCRPHLHGRHCQELQSGYFCATLDQATAEAEHGRSLQPADPQLPGAPWPAPRYCTPAPGTSSGWLGPRLWRQRDPDCVLQRARQARYSHQLPRTPGKPPPLHQGSGFALGRGSDFARVVDGAGLSLLAPTVPRALDYDIVLRYETQAPEVWQALVRVRAQSQPRSTRCARPLPSEQLFQATLTHARRAVVLSRPFCFEPGTRYSVTLRLWRTKEGRRPEGGTILLDSVVLLPRVGELPGLRSVDPGALRRLQELHEAGCLEAARVSPPQSMPEACARLACSISALLHGGGLACECHLQGSLSTECAPLGGQCPCRPNITGRTCDRCLPGTFGLGPTGCHECRCHPEGAASAVCNSTSGQCTCWAGCAGRRCDRCLSGRWGFPRCQPCACNGHAELCHPLTGVCQDCRGATTGRHCERCLDGYYGDPTLGSGQRCQPCPCPGHPGSGLYHGTSCHVDSTSGRVLCLCAPGYAGPRCDRCSPGYFGRPWPGDDPRRSPCRPCQCNNNIDPRDPAACDPHSGHCQRCLHHSHGAGCAHCRPGFHGSALRPGGCRRCSCDPRGTSPVRCPPEAEACFCDPVSGQCPCRPHTLGRDCSRCAPLFWNLGGPRGCEPCSCHARHTLQPGCHPVTGQCPCRAGFGGRTCSRCQDGYWGDPEQECRACACDPQGSISPSCDPHTGTCRCREGISGPRCQACARGSTGGFPHCTSCPPCFASWDQRLAPLQLHLDTMVHEVAALRQGMPGWGAGLRGDQLQALEGKLQQAQTLLESPSPTRGPLQQFTEWITGLRQGVWGH

Target ORF information:

RefSeq Version	XM_015459647.1
Organism	Bos taurus(cattle)
Definition	Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459647.1

ORF Insert Sequence:

ATGCCTGATG CTGGCTCCCA CTGCCCAGGA GCCCCAGCCG GGCTAGGCCC CCTTCTCTTA 
GCCCTGCTGC TGCTGCTGCC ACCCATGGTC CCGGCTCCCA GAACAAGTGC CCAGGAGGTA 
CCAGACATGA TGCCTCCTCC CGGCTGCTCA GGTGGCAACT GCCACCCACC TACTGGCAAC 
CTGGTTATTG GCCGTGGCCA GAGCCTTCGA ACCTCATCTA CCTGTGGCCT CCATGGCCCT 
GAACTCTACT GTGTTGTCAG TAAACTACAG GCGCTTCTCC AGGACTCTCA GAATTGCTGC 
TTTTGTGATT CTCGGGATGG GAAAAGCCAT GGCATTGAGA ATGTGGTCTC CCGGAGTGAC 
CCTGATGGCA GAAAGACCTG GTGGCAAGCA GAGAGTGGTG TTGAGAATGT CACCATCCAG 
TTGGACCTAG AAGGTGCCTT TTACTTTACC CACCTCATCA TGACTTTTAA GACATTCCGC 
CCAGCAGCTC TGCTCCTTGA GCGCTCAGTG GACCATGGCC ACTCCTGGCA TGTGTTCCGC 
TACTTTGCCC ACAACTGCTC AGGCCTCTTT CCTGGGATCC CTCCTGCCCC TGGCCGCAGA 
GTCAGTGACC TCGTCTGTGA CCAGCGTTAC TCAGACATCG AGCCTGCCAC TGAGGGAGAG 
GTAATTTTCC AAGTTCTGGA CCCATCCTTT CTGGTGGAGA ACACAAACAA CGCGGAGATT 
CAGGAACTCC TGCGTGTGAC TAACCTCCGT GTGAACTTCT CCAAGCTGCA CACACTGGGT 
GACAGGCCCC TGGGGGGCCT CAGGGGCCAC CCCTTCTACT ACTATGCTCT CTATGAGCTA 
GTGATTGCAG GCAGCTGCCT GTGCCACGGC CATGCTTCCG AGTGCAGGCC CGCACCCGGG 
TCCCCACCCA GCGTGGAAGG CATGGTGCAT GGCCATTGTG TCTGCCGCCA CCACACCACT 
GGTACCCACT GTGAACGTTG CCAGGGCCTG TACCAGGACC ACCCGTGGCA GGCAGCAGAG 
CCTGGGTACC CCCACACCTG CCAGGAATGC AAGTGCCATG GGCACGCCCG CAGTTGTCAC 
TTTGACATGG CCCTGTATCT CGCATCTGGC AACGTGAGTG GAGGTGTGTG CGATGCATGT 
CAACACAACA CGGCCGGGCG CCACTGTGAG CTCTGCCAGC CCTTCTTCCA CCGGGACCCC 
CTGGAGGACC CCCGCTCCCT TCACTCCTGC AAACCCTGTG ACTGCAACCC TATGGGTGCC 
CTGGAAGGGG GTTTGTGTGA TGCCTACACA GACACCACCC GGGGTCTCCT CTCTGGGCAG 
TGTCGCTGCA AAGTTCACGT CTGGGGCCAG CGCTGTGACT CCTGCCGGCC AGGTCACTAT 
GGCCTGAGCC TCACCCAGTC AGAAGGCTGC CAGCCCTGCA GGTGCAACGC CCGGGGCCGG 
GTCCCCAGCA CTCAGGCCTG TGATCCATCC AGCGGTGCCT GTCACTGCAA ACGCTTTGTC 
TCTGGACGGG ACTGCAGCCG CTGTCTGCCT GAGTTCTGGG GTCTGAGCAG TGACTCTCTA 
GGCTGTCGGC CCTGTGACTG TGACTTTGGG GGTGCCTACA GCAACAGGTG TTCTGCAGGG 
CAGGGCCTCT GCCTCTGCCG CCCCCACCTG CATGGCCGCC ACTGCCAGGA ACTCCAGTCT 
GGGTACTTCT GCGCCACCCT CGACCAGGCC ACTGCCGAAG CAGAGCATGG CCGCAGCCTC 
CAGCCTGCTG ACCCCCAGCT GCCTGGAGCC CCCTGGCCTG CTCCCCGCTA CTGTACACCA 
GCCCCAGGGA CCTCCTCTGG TTGGCTCGGG CCCCGACTTT GGAGACAGCG TGACCCTGAC 
TGTGTCCTCC AACGTGCCAG GCAAGCCCGG TACAGCCACC AGCTGCCCAG GACCCCAGGG 
AAGCCCCCGC CCCTGCATCA GGGATCTGGC TTTGCACTGG GGAGGGGCTC AGACTTCGCA 
CGTGTGGTGG ACGGGGCTGG CCTCTCTCTG CTGGCACCCA CAGTGCCTCG CGCCCTGGAC 
TATGACATCG TCCTACGCTA TGAGACCCAG GCACCTGAAG TGTGGCAGGC ATTAGTCCGG 
GTCCGTGCCC AGTCACAGCC CCGCAGTACC CGCTGTGCCC GTCCGTTGCC CTCAGAGCAG 
CTGTTCCAGG CGACCTTGAC CCATGCGCGC AGAGCCGTGG TTCTTTCCAG ACCGTTCTGC 
TTTGAACCCG GAACCCGCTA CTCCGTGACC CTGCGGCTGT GGCGGACCAA GGAGGGACGG 
AGACCCGAAG GGGGCACGAT CCTCCTGGAT TCAGTAGTGC TCCTGCCGCG GGTTGGAGAG 
TTGCCTGGAC TGAGGTCCGT GGACCCGGGG GCCCTGAGGC GCTTGCAGGA GCTCCACGAG 
GCGGGCTGCC TGGAGGCAGC GAGGGTGAGC CCACCCCAAA GCATGCCTGA GGCCTGTGCC 
CGCCTCGCCT GCAGCATCTC AGCCCTGCTC CATGGAGGTG GCCTCGCATG TGAGTGCCAC 
CTGCAGGGTT CACTGAGCAC CGAGTGTGCC CCACTGGGGG GGCAATGCCC CTGCCGCCCT 
AATATCACAG GTCGTACCTG TGACCGCTGC TTGCCTGGGA CATTTGGCCT CGGGCCCACC 
GGGTGCCATG AGTGCCGCTG CCACCCCGAG GGGGCTGCCA GTGCCGTCTG TAACTCTACG 
AGTGGGCAGT GCACGTGTTG GGCAGGCTGT GCTGGTCGCC GCTGTGACCG TTGCCTCTCT 
GGCCGGTGGG GCTTCCCACG CTGCCAGCCC TGTGCCTGCA ATGGACACGC CGAGTTGTGC 
CACCCACTCA CAGGCGTCTG CCAGGACTGC CGCGGAGCCA CCACAGGCCG GCATTGTGAG 
AGGTGCTTGG ATGGCTACTA TGGAGACCCC ACCCTGGGCT CAGGCCAGCG GTGCCAGCCC 
TGCCCCTGTC CTGGGCACCC TGGCTCAGGC CTCTATCATG GGACCTCCTG CCATGTGGAC 
AGCACCAGTG GACGTGTCCT GTGCCTCTGT GCCCCCGGCT ATGCAGGACC CCGCTGTGAC 
CGCTGCTCCC CCGGCTACTT TGGGCGCCCC TGGCCTGGAG ATGACCCCAG AAGGAGTCCC 
TGCCGGCCCT GCCAGTGCAA CAACAATATT GATCCCCGTG ACCCAGCCGC CTGTGACCCC 
CACAGCGGAC ATTGCCAGCG CTGTCTGCAC CACAGCCACG GCGCTGGCTG TGCCCACTGC 
CGGCCTGGCT TCCACGGCAG TGCCCTGCGC CCAGGGGGCT GCCGGCGCTG CAGCTGTGAC 
CCCCGGGGCA CCAGCCCTGT AAGGTGCCCA CCTGAGGCTG AAGCCTGCTT CTGTGACCCG 
GTCAGCGGGC AGTGCCCCTG CCGCCCCCAC ACACTGGGGC GGGACTGTAG CCGCTGTGCC 
CCCCTCTTCT GGAACCTCGG GGGGCCCCGG GGCTGTGAGC CCTGCAGCTG CCACGCCCGA 
CACACTTTGC AGCCGGGGTG TCACCCGGTC ACGGGTCAGT GCCCCTGCCG GGCAGGATTT 
GGGGGCCGCA CGTGCTCCCG GTGTCAGGAT GGGTACTGGG GTGACCCGGA GCAGGAGTGC 
AGAGCCTGTG CCTGTGACCC CCAGGGCTCC ATCTCGCCCA GCTGTGACCC GCACACAGGC 
ACCTGCCGCT GCCGAGAGGG TATCTCAGGG CCGAGGTGCC AGGCGTGTGC CCGTGGCTCC 
ACAGGAGGCT TCCCACACTG CACGTCCTGC CCTCCCTGCT TCGCCTCCTG GGACCAACGC 
CTGGCTCCCC TCCAGCTGCA TCTGGACACC ATGGTCCATG AGGTGGCTGC CCTGCGCCAG 
GGCATGCCTG GCTGGGGTGC TGGGCTCCGG GGAGATCAGC TGCAGGCCCT GGAGGGTAAA 
CTCCAGCAGG CTCAGACACT CCTGGAATCA CCTTCCCCCA CCAGAGGTCC CCTGCAACAA 
TTTACAGAGT GGATCACCGG ACTCAGGCAA GGGGTCTGGG GTCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC530599 ( XM_015459648.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315134.1 Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X2, mRNA.

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CloneID	OBa94462
Clone ID Related Accession (Same CDS sequence)	XM_015459648.1 , XM_015468731.1
Accession Version	XM_015459648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	laminin subunit beta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATG CTGGCTCCCA CTGCCCAGGA GCCCCAGCCG GGCTAGGCCC CCTTCTCTTA 
GCCCTGCTGC TGCTGCTGCC ACCCATGGTC CCGGCTCCCA GAACAAGTGC CCAGGAGGTA 
CCAGACATGA TGCCTCCTCC CGGCTGCTCA GGTGGCAACT GCCACCCACC TACTGGCAAC 
CTGGTTATTG GCCGTGGCCA GAGCCTTCGA ACCTCATCTA CCTGTGGCCT CCATGGCCCT 
GAACTCTACT GTGTTGTCAG TAAACTACAG GACTCTCAGA ATTGCTGCTT TTGTGATTCT 
CGGGATGGGA AAAGCCATGG CATTGAGAAT GTGGTCTCCC GGAGTGACCC TGATGGCAGA 
AAGACCTGGT GGCAAGCAGA GAGTGGTGTT GAGAATGTCA CCATCCAGTT GGACCTAGAA 
GGTGCCTTTT ACTTTACCCA CCTCATCATG ACTTTTAAGA CATTCCGCCC AGCAGCTCTG 
CTCCTTGAGC GCTCAGTGGA CCATGGCCAC TCCTGGCATG TGTTCCGCTA CTTTGCCCAC 
AACTGCTCAG GCCTCTTTCC TGGGATCCCT CCTGCCCCTG GCCGCAGAGT CAGTGACCTC 
GTCTGTGACC AGCGTTACTC AGACATCGAG CCTGCCACTG AGGGAGAGGT AATTTTCCAA 
GTTCTGGACC CATCCTTTCT GGTGGAGAAC ACAAACAACG CGGAGATTCA GGAACTCCTG 
CGTGTGACTA ACCTCCGTGT GAACTTCTCC AAGCTGCACA CACTGGGTGA CAGGCCCCTG 
GGGGGCCTCA GGGGCCACCC CTTCTACTAC TATGCTCTCT ATGAGCTAGT GATTGCAGGC 
AGCTGCCTGT GCCACGGCCA TGCTTCCGAG TGCAGGCCCG CACCCGGGTC CCCACCCAGC 
GTGGAAGGCA TGGTGCATGG CCATTGTGTC TGCCGCCACC ACACCACTGG TACCCACTGT 
GAACGTTGCC AGGGCCTGTA CCAGGACCAC CCGTGGCAGG CAGCAGAGCC TGGGTACCCC 
CACACCTGCC AGGAATGCAA GTGCCATGGG CACGCCCGCA GTTGTCACTT TGACATGGCC 
CTGTATCTCG CATCTGGCAA CGTGAGTGGA GGTGTGTGCG ATGCATGTCA ACACAACACG 
GCCGGGCGCC ACTGTGAGCT CTGCCAGCCC TTCTTCCACC GGGACCCCCT GGAGGACCCC 
CGCTCCCTTC ACTCCTGCAA ACCCTGTGAC TGCAACCCTA TGGGTGCCCT GGAAGGGGGT 
TTGTGTGATG CCTACACAGA CACCACCCGG GGTCTCCTCT CTGGGCAGTG TCGCTGCAAA 
GTTCACGTCT GGGGCCAGCG CTGTGACTCC TGCCGGCCAG GTCACTATGG CCTGAGCCTC 
ACCCAGTCAG AAGGCTGCCA GCCCTGCAGG TGCAACGCCC GGGGCCGGGT CCCCAGCACT 
CAGGCCTGTG ATCCATCCAG CGGTGCCTGT CACTGCAAAC GCTTTGTCTC TGGACGGGAC 
TGCAGCCGCT GTCTGCCTGA GTTCTGGGGT CTGAGCAGTG ACTCTCTAGG CTGTCGGCCC 
TGTGACTGTG ACTTTGGGGG TGCCTACAGC AACAGGTGTT CTGCAGGGCA GGGCCTCTGC 
CTCTGCCGCC CCCACCTGCA TGGCCGCCAC TGCCAGGAAC TCCAGTCTGG GTACTTCTGC 
GCCACCCTCG ACCAGGCCAC TGCCGAAGCA GAGCATGGCC GCAGCCTCCA GCCTGCTGAC 
CCCCAGCTGC CTGGAGCCCC CTGGCCTGCT CCCCGCTACT GTACACCAGC CCCAGGGACC 
TCCTCTGGTT GGCTCGGGCC CCGACTTTGG AGACAGCGTG ACCCTGACTG TGTCCTCCAA 
CGTGCCAGGC AAGCCCGGTA CAGCCACCAG CTGCCCAGGA CCCCAGGGAA GCCCCCGCCC 
CTGCATCAGG GATCTGGCTT TGCACTGGGG AGGGGCTCAG ACTTCGCACG TGTGGTGGAC 
GGGGCTGGCC TCTCTCTGCT GGCACCCACA GTGCCTCGCG CCCTGGACTA TGACATCGTC 
CTACGCTATG AGACCCAGGC ACCTGAAGTG TGGCAGGCAT TAGTCCGGGT CCGTGCCCAG 
TCACAGCCCC GCAGTACCCG CTGTGCCCGT CCGTTGCCCT CAGAGCAGCT GTTCCAGGCG 
ACCTTGACCC ATGCGCGCAG AGCCGTGGTT CTTTCCAGAC CGTTCTGCTT TGAACCCGGA 
ACCCGCTACT CCGTGACCCT GCGGCTGTGG CGGACCAAGG AGGGACGGAG ACCCGAAGGG 
GGCACGATCC TCCTGGATTC AGTAGTGCTC CTGCCGCGGG TTGGAGAGTT GCCTGGACTG 
AGGTCCGTGG ACCCGGGGGC CCTGAGGCGC TTGCAGGAGC TCCACGAGGC GGGCTGCCTG 
GAGGCAGCGA GGGTGAGCCC ACCCCAAAGC ATGCCTGAGG CCTGTGCCCG CCTCGCCTGC 
AGCATCTCAG CCCTGCTCCA TGGAGGTGGC CTCGCATGTG AGTGCCACCT GCAGGGTTCA 
CTGAGCACCG AGTGTGCCCC ACTGGGGGGG CAATGCCCCT GCCGCCCTAA TATCACAGGT 
CGTACCTGTG ACCGCTGCTT GCCTGGGACA TTTGGCCTCG GGCCCACCGG GTGCCATGAG 
TGCCGCTGCC ACCCCGAGGG GGCTGCCAGT GCCGTCTGTA ACTCTACGAG TGGGCAGTGC 
ACGTGTTGGG CAGGCTGTGC TGGTCGCCGC TGTGACCGTT GCCTCTCTGG CCGGTGGGGC 
TTCCCACGCT GCCAGCCCTG TGCCTGCAAT GGACACGCCG AGTTGTGCCA CCCACTCACA 
GGCGTCTGCC AGGACTGCCG CGGAGCCACC ACAGGCCGGC ATTGTGAGAG GTGCTTGGAT 
GGCTACTATG GAGACCCCAC CCTGGGCTCA GGCCAGCGGT GCCAGCCCTG CCCCTGTCCT 
GGGCACCCTG GCTCAGGCCT CTATCATGGG ACCTCCTGCC ATGTGGACAG CACCAGTGGA 
CGTGTCCTGT GCCTCTGTGC CCCCGGCTAT GCAGGACCCC GCTGTGACCG CTGCTCCCCC 
GGCTACTTTG GGCGCCCCTG GCCTGGAGAT GACCCCAGAA GGAGTCCCTG CCGGCCCTGC 
CAGTGCAACA ACAATATTGA TCCCCGTGAC CCAGCCGCCT GTGACCCCCA CAGCGGACAT 
TGCCAGCGCT GTCTGCACCA CAGCCACGGC GCTGGCTGTG CCCACTGCCG GCCTGGCTTC 
CACGGCAGTG CCCTGCGCCC AGGGGGCTGC CGGCGCTGCA GCTGTGACCC CCGGGGCACC 
AGCCCTGTAA GGTGCCCACC TGAGGCTGAA GCCTGCTTCT GTGACCCGGT CAGCGGGCAG 
TGCCCCTGCC GCCCCCACAC ACTGGGGCGG GACTGTAGCC GCTGTGCCCC CCTCTTCTGG 
AACCTCGGGG GGCCCCGGGG CTGTGAGCCC TGCAGCTGCC ACGCCCGACA CACTTTGCAG 
CCGGGGTGTC ACCCGGTCAC GGGTCAGTGC CCCTGCCGGG CAGGATTTGG GGGCCGCACG 
TGCTCCCGGT GTCAGGATGG GTACTGGGGT GACCCGGAGC AGGAGTGCAG AGCCTGTGCC 
TGTGACCCCC AGGGCTCCAT CTCGCCCAGC TGTGACCCGC ACACAGGCAC CTGCCGCTGC 
CGAGAGGGTA TCTCAGGGCC GAGGTGCCAG GCGTGTGCCC GTGGCTCCAC AGGAGGCTTC 
CCACACTGCA CGTCCTGCCC TCCCTGCTTC GCCTCCTGGG ACCAACGCCT GGCTCCCCTC 
CAGCTGCATC TGGACACCAT GGTCCATGAG GTGGCTGCCC TGCGCCAGGG CATGCCTGGC 
TGGGGTGCTG GGCTCCGGGG AGATCAGCTG CAGGCCCTGG AGGGTAAACT CCAGCAGGCT 
CAGACACTCC TGGAATCACC TTCCCCCACC AGAGGTCCCC TGCAACAATT TACAGAGTGG 
ATCACCGGAC TCAGGCAAGG GGTCTGGGGT CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315134.1
CDS	1033..5028
Translation	MPDAGSHCPGAPAGLGPLLLALLLLLPPMVPAPRTSAQEVPDMMPPPGCSGGNCHPPTGNLVIGRGQSLRTSSTCGLHGPELYCVVSKLQDSQNCCFCDSRDGKSHGIENVVSRSDPDGRKTWWQAESGVENVTIQLDLEGAFYFTHLIMTFKTFRPAALLLERSVDHGHSWHVFRYFAHNCSGLFPGIPPAPGRRVSDLVCDQRYSDIEPATEGEVIFQVLDPSFLVENTNNAEIQELLRVTNLRVNFSKLHTLGDRPLGGLRGHPFYYYALYELVIAGSCLCHGHASECRPAPGSPPSVEGMVHGHCVCRHHTTGTHCERCQGLYQDHPWQAAEPGYPHTCQECKCHGHARSCHFDMALYLASGNVSGGVCDACQHNTAGRHCELCQPFFHRDPLEDPRSLHSCKPCDCNPMGALEGGLCDAYTDTTRGLLSGQCRCKVHVWGQRCDSCRPGHYGLSLTQSEGCQPCRCNARGRVPSTQACDPSSGACHCKRFVSGRDCSRCLPEFWGLSSDSLGCRPCDCDFGGAYSNRCSAGQGLCLCRPHLHGRHCQELQSGYFCATLDQATAEAEHGRSLQPADPQLPGAPWPAPRYCTPAPGTSSGWLGPRLWRQRDPDCVLQRARQARYSHQLPRTPGKPPPLHQGSGFALGRGSDFARVVDGAGLSLLAPTVPRALDYDIVLRYETQAPEVWQALVRVRAQSQPRSTRCARPLPSEQLFQATLTHARRAVVLSRPFCFEPGTRYSVTLRLWRTKEGRRPEGGTILLDSVVLLPRVGELPGLRSVDPGALRRLQELHEAGCLEAARVSPPQSMPEACARLACSISALLHGGGLACECHLQGSLSTECAPLGGQCPCRPNITGRTCDRCLPGTFGLGPTGCHECRCHPEGAASAVCNSTSGQCTCWAGCAGRRCDRCLSGRWGFPRCQPCACNGHAELCHPLTGVCQDCRGATTGRHCERCLDGYYGDPTLGSGQRCQPCPCPGHPGSGLYHGTSCHVDSTSGRVLCLCAPGYAGPRCDRCSPGYFGRPWPGDDPRRSPCRPCQCNNNIDPRDPAACDPHSGHCQRCLHHSHGAGCAHCRPGFHGSALRPGGCRRCSCDPRGTSPVRCPPEAEACFCDPVSGQCPCRPHTLGRDCSRCAPLFWNLGGPRGCEPCSCHARHTLQPGCHPVTGQCPCRAGFGGRTCSRCQDGYWGDPEQECRACACDPQGSISPSCDPHTGTCRCREGISGPRCQACARGSTGGFPHCTSCPPCFASWDQRLAPLQLHLDTMVHEVAALRQGMPGWGAGLRGDQLQALEGKLQQAQTLLESPSPTRGPLQQFTEWITGLRQGVWGH

Target ORF information:

RefSeq Version	XM_015459648.1
Organism	Bos taurus(cattle)
Definition	Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459648.1

ORF Insert Sequence:

ATGCCTGATG CTGGCTCCCA CTGCCCAGGA GCCCCAGCCG GGCTAGGCCC CCTTCTCTTA 
GCCCTGCTGC TGCTGCTGCC ACCCATGGTC CCGGCTCCCA GAACAAGTGC CCAGGAGGTA 
CCAGACATGA TGCCTCCTCC CGGCTGCTCA GGTGGCAACT GCCACCCACC TACTGGCAAC 
CTGGTTATTG GCCGTGGCCA GAGCCTTCGA ACCTCATCTA CCTGTGGCCT CCATGGCCCT 
GAACTCTACT GTGTTGTCAG TAAACTACAG GACTCTCAGA ATTGCTGCTT TTGTGATTCT 
CGGGATGGGA AAAGCCATGG CATTGAGAAT GTGGTCTCCC GGAGTGACCC TGATGGCAGA 
AAGACCTGGT GGCAAGCAGA GAGTGGTGTT GAGAATGTCA CCATCCAGTT GGACCTAGAA 
GGTGCCTTTT ACTTTACCCA CCTCATCATG ACTTTTAAGA CATTCCGCCC AGCAGCTCTG 
CTCCTTGAGC GCTCAGTGGA CCATGGCCAC TCCTGGCATG TGTTCCGCTA CTTTGCCCAC 
AACTGCTCAG GCCTCTTTCC TGGGATCCCT CCTGCCCCTG GCCGCAGAGT CAGTGACCTC 
GTCTGTGACC AGCGTTACTC AGACATCGAG CCTGCCACTG AGGGAGAGGT AATTTTCCAA 
GTTCTGGACC CATCCTTTCT GGTGGAGAAC ACAAACAACG CGGAGATTCA GGAACTCCTG 
CGTGTGACTA ACCTCCGTGT GAACTTCTCC AAGCTGCACA CACTGGGTGA CAGGCCCCTG 
GGGGGCCTCA GGGGCCACCC CTTCTACTAC TATGCTCTCT ATGAGCTAGT GATTGCAGGC 
AGCTGCCTGT GCCACGGCCA TGCTTCCGAG TGCAGGCCCG CACCCGGGTC CCCACCCAGC 
GTGGAAGGCA TGGTGCATGG CCATTGTGTC TGCCGCCACC ACACCACTGG TACCCACTGT 
GAACGTTGCC AGGGCCTGTA CCAGGACCAC CCGTGGCAGG CAGCAGAGCC TGGGTACCCC 
CACACCTGCC AGGAATGCAA GTGCCATGGG CACGCCCGCA GTTGTCACTT TGACATGGCC 
CTGTATCTCG CATCTGGCAA CGTGAGTGGA GGTGTGTGCG ATGCATGTCA ACACAACACG 
GCCGGGCGCC ACTGTGAGCT CTGCCAGCCC TTCTTCCACC GGGACCCCCT GGAGGACCCC 
CGCTCCCTTC ACTCCTGCAA ACCCTGTGAC TGCAACCCTA TGGGTGCCCT GGAAGGGGGT 
TTGTGTGATG CCTACACAGA CACCACCCGG GGTCTCCTCT CTGGGCAGTG TCGCTGCAAA 
GTTCACGTCT GGGGCCAGCG CTGTGACTCC TGCCGGCCAG GTCACTATGG CCTGAGCCTC 
ACCCAGTCAG AAGGCTGCCA GCCCTGCAGG TGCAACGCCC GGGGCCGGGT CCCCAGCACT 
CAGGCCTGTG ATCCATCCAG CGGTGCCTGT CACTGCAAAC GCTTTGTCTC TGGACGGGAC 
TGCAGCCGCT GTCTGCCTGA GTTCTGGGGT CTGAGCAGTG ACTCTCTAGG CTGTCGGCCC 
TGTGACTGTG ACTTTGGGGG TGCCTACAGC AACAGGTGTT CTGCAGGGCA GGGCCTCTGC 
CTCTGCCGCC CCCACCTGCA TGGCCGCCAC TGCCAGGAAC TCCAGTCTGG GTACTTCTGC 
GCCACCCTCG ACCAGGCCAC TGCCGAAGCA GAGCATGGCC GCAGCCTCCA GCCTGCTGAC 
CCCCAGCTGC CTGGAGCCCC CTGGCCTGCT CCCCGCTACT GTACACCAGC CCCAGGGACC 
TCCTCTGGTT GGCTCGGGCC CCGACTTTGG AGACAGCGTG ACCCTGACTG TGTCCTCCAA 
CGTGCCAGGC AAGCCCGGTA CAGCCACCAG CTGCCCAGGA CCCCAGGGAA GCCCCCGCCC 
CTGCATCAGG GATCTGGCTT TGCACTGGGG AGGGGCTCAG ACTTCGCACG TGTGGTGGAC 
GGGGCTGGCC TCTCTCTGCT GGCACCCACA GTGCCTCGCG CCCTGGACTA TGACATCGTC 
CTACGCTATG AGACCCAGGC ACCTGAAGTG TGGCAGGCAT TAGTCCGGGT CCGTGCCCAG 
TCACAGCCCC GCAGTACCCG CTGTGCCCGT CCGTTGCCCT CAGAGCAGCT GTTCCAGGCG 
ACCTTGACCC ATGCGCGCAG AGCCGTGGTT CTTTCCAGAC CGTTCTGCTT TGAACCCGGA 
ACCCGCTACT CCGTGACCCT GCGGCTGTGG CGGACCAAGG AGGGACGGAG ACCCGAAGGG 
GGCACGATCC TCCTGGATTC AGTAGTGCTC CTGCCGCGGG TTGGAGAGTT GCCTGGACTG 
AGGTCCGTGG ACCCGGGGGC CCTGAGGCGC TTGCAGGAGC TCCACGAGGC GGGCTGCCTG 
GAGGCAGCGA GGGTGAGCCC ACCCCAAAGC ATGCCTGAGG CCTGTGCCCG CCTCGCCTGC 
AGCATCTCAG CCCTGCTCCA TGGAGGTGGC CTCGCATGTG AGTGCCACCT GCAGGGTTCA 
CTGAGCACCG AGTGTGCCCC ACTGGGGGGG CAATGCCCCT GCCGCCCTAA TATCACAGGT 
CGTACCTGTG ACCGCTGCTT GCCTGGGACA TTTGGCCTCG GGCCCACCGG GTGCCATGAG 
TGCCGCTGCC ACCCCGAGGG GGCTGCCAGT GCCGTCTGTA ACTCTACGAG TGGGCAGTGC 
ACGTGTTGGG CAGGCTGTGC TGGTCGCCGC TGTGACCGTT GCCTCTCTGG CCGGTGGGGC 
TTCCCACGCT GCCAGCCCTG TGCCTGCAAT GGACACGCCG AGTTGTGCCA CCCACTCACA 
GGCGTCTGCC AGGACTGCCG CGGAGCCACC ACAGGCCGGC ATTGTGAGAG GTGCTTGGAT 
GGCTACTATG GAGACCCCAC CCTGGGCTCA GGCCAGCGGT GCCAGCCCTG CCCCTGTCCT 
GGGCACCCTG GCTCAGGCCT CTATCATGGG ACCTCCTGCC ATGTGGACAG CACCAGTGGA 
CGTGTCCTGT GCCTCTGTGC CCCCGGCTAT GCAGGACCCC GCTGTGACCG CTGCTCCCCC 
GGCTACTTTG GGCGCCCCTG GCCTGGAGAT GACCCCAGAA GGAGTCCCTG CCGGCCCTGC 
CAGTGCAACA ACAATATTGA TCCCCGTGAC CCAGCCGCCT GTGACCCCCA CAGCGGACAT 
TGCCAGCGCT GTCTGCACCA CAGCCACGGC GCTGGCTGTG CCCACTGCCG GCCTGGCTTC 
CACGGCAGTG CCCTGCGCCC AGGGGGCTGC CGGCGCTGCA GCTGTGACCC CCGGGGCACC 
AGCCCTGTAA GGTGCCCACC TGAGGCTGAA GCCTGCTTCT GTGACCCGGT CAGCGGGCAG 
TGCCCCTGCC GCCCCCACAC ACTGGGGCGG GACTGTAGCC GCTGTGCCCC CCTCTTCTGG 
AACCTCGGGG GGCCCCGGGG CTGTGAGCCC TGCAGCTGCC ACGCCCGACA CACTTTGCAG 
CCGGGGTGTC ACCCGGTCAC GGGTCAGTGC CCCTGCCGGG CAGGATTTGG GGGCCGCACG 
TGCTCCCGGT GTCAGGATGG GTACTGGGGT GACCCGGAGC AGGAGTGCAG AGCCTGTGCC 
TGTGACCCCC AGGGCTCCAT CTCGCCCAGC TGTGACCCGC ACACAGGCAC CTGCCGCTGC 
CGAGAGGGTA TCTCAGGGCC GAGGTGCCAG GCGTGTGCCC GTGGCTCCAC AGGAGGCTTC 
CCACACTGCA CGTCCTGCCC TCCCTGCTTC GCCTCCTGGG ACCAACGCCT GGCTCCCCTC 
CAGCTGCATC TGGACACCAT GGTCCATGAG GTGGCTGCCC TGCGCCAGGG CATGCCTGGC 
TGGGGTGCTG GGCTCCGGGG AGATCAGCTG CAGGCCCTGG AGGGTAAACT CCAGCAGGCT 
CAGACACTCC TGGAATCACC TTCCCCCACC AGAGGTCCCC TGCAACAATT TACAGAGTGG 
ATCACCGGAC TCAGGCAAGG GGTCTGGGGT CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC530599 ( XM_015468731.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324217.1 Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X2, mRNA.

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CloneID	OBa94462
Clone ID Related Accession (Same CDS sequence)	XM_015459648.1 , XM_015468731.1
Accession Version	XM_015468731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	laminin subunit beta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATG CTGGCTCCCA CTGCCCAGGA GCCCCAGCCG GGCTAGGCCC CCTTCTCTTA 
GCCCTGCTGC TGCTGCTGCC ACCCATGGTC CCGGCTCCCA GAACAAGTGC CCAGGAGGTA 
CCAGACATGA TGCCTCCTCC CGGCTGCTCA GGTGGCAACT GCCACCCACC TACTGGCAAC 
CTGGTTATTG GCCGTGGCCA GAGCCTTCGA ACCTCATCTA CCTGTGGCCT CCATGGCCCT 
GAACTCTACT GTGTTGTCAG TAAACTACAG GACTCTCAGA ATTGCTGCTT TTGTGATTCT 
CGGGATGGGA AAAGCCATGG CATTGAGAAT GTGGTCTCCC GGAGTGACCC TGATGGCAGA 
AAGACCTGGT GGCAAGCAGA GAGTGGTGTT GAGAATGTCA CCATCCAGTT GGACCTAGAA 
GGTGCCTTTT ACTTTACCCA CCTCATCATG ACTTTTAAGA CATTCCGCCC AGCAGCTCTG 
CTCCTTGAGC GCTCAGTGGA CCATGGCCAC TCCTGGCATG TGTTCCGCTA CTTTGCCCAC 
AACTGCTCAG GCCTCTTTCC TGGGATCCCT CCTGCCCCTG GCCGCAGAGT CAGTGACCTC 
GTCTGTGACC AGCGTTACTC AGACATCGAG CCTGCCACTG AGGGAGAGGT AATTTTCCAA 
GTTCTGGACC CATCCTTTCT GGTGGAGAAC ACAAACAACG CGGAGATTCA GGAACTCCTG 
CGTGTGACTA ACCTCCGTGT GAACTTCTCC AAGCTGCACA CACTGGGTGA CAGGCCCCTG 
GGGGGCCTCA GGGGCCACCC CTTCTACTAC TATGCTCTCT ATGAGCTAGT GATTGCAGGC 
AGCTGCCTGT GCCACGGCCA TGCTTCCGAG TGCAGGCCCG CACCCGGGTC CCCACCCAGC 
GTGGAAGGCA TGGTGCATGG CCATTGTGTC TGCCGCCACC ACACCACTGG TACCCACTGT 
GAACGTTGCC AGGGCCTGTA CCAGGACCAC CCGTGGCAGG CAGCAGAGCC TGGGTACCCC 
CACACCTGCC AGGAATGCAA GTGCCATGGG CACGCCCGCA GTTGTCACTT TGACATGGCC 
CTGTATCTCG CATCTGGCAA CGTGAGTGGA GGTGTGTGCG ATGCATGTCA ACACAACACG 
GCCGGGCGCC ACTGTGAGCT CTGCCAGCCC TTCTTCCACC GGGACCCCCT GGAGGACCCC 
CGCTCCCTTC ACTCCTGCAA ACCCTGTGAC TGCAACCCTA TGGGTGCCCT GGAAGGGGGT 
TTGTGTGATG CCTACACAGA CACCACCCGG GGTCTCCTCT CTGGGCAGTG TCGCTGCAAA 
GTTCACGTCT GGGGCCAGCG CTGTGACTCC TGCCGGCCAG GTCACTATGG CCTGAGCCTC 
ACCCAGTCAG AAGGCTGCCA GCCCTGCAGG TGCAACGCCC GGGGCCGGGT CCCCAGCACT 
CAGGCCTGTG ATCCATCCAG CGGTGCCTGT CACTGCAAAC GCTTTGTCTC TGGACGGGAC 
TGCAGCCGCT GTCTGCCTGA GTTCTGGGGT CTGAGCAGTG ACTCTCTAGG CTGTCGGCCC 
TGTGACTGTG ACTTTGGGGG TGCCTACAGC AACAGGTGTT CTGCAGGGCA GGGCCTCTGC 
CTCTGCCGCC CCCACCTGCA TGGCCGCCAC TGCCAGGAAC TCCAGTCTGG GTACTTCTGC 
GCCACCCTCG ACCAGGCCAC TGCCGAAGCA GAGCATGGCC GCAGCCTCCA GCCTGCTGAC 
CCCCAGCTGC CTGGAGCCCC CTGGCCTGCT CCCCGCTACT GTACACCAGC CCCAGGGACC 
TCCTCTGGTT GGCTCGGGCC CCGACTTTGG AGACAGCGTG ACCCTGACTG TGTCCTCCAA 
CGTGCCAGGC AAGCCCGGTA CAGCCACCAG CTGCCCAGGA CCCCAGGGAA GCCCCCGCCC 
CTGCATCAGG GATCTGGCTT TGCACTGGGG AGGGGCTCAG ACTTCGCACG TGTGGTGGAC 
GGGGCTGGCC TCTCTCTGCT GGCACCCACA GTGCCTCGCG CCCTGGACTA TGACATCGTC 
CTACGCTATG AGACCCAGGC ACCTGAAGTG TGGCAGGCAT TAGTCCGGGT CCGTGCCCAG 
TCACAGCCCC GCAGTACCCG CTGTGCCCGT CCGTTGCCCT CAGAGCAGCT GTTCCAGGCG 
ACCTTGACCC ATGCGCGCAG AGCCGTGGTT CTTTCCAGAC CGTTCTGCTT TGAACCCGGA 
ACCCGCTACT CCGTGACCCT GCGGCTGTGG CGGACCAAGG AGGGACGGAG ACCCGAAGGG 
GGCACGATCC TCCTGGATTC AGTAGTGCTC CTGCCGCGGG TTGGAGAGTT GCCTGGACTG 
AGGTCCGTGG ACCCGGGGGC CCTGAGGCGC TTGCAGGAGC TCCACGAGGC GGGCTGCCTG 
GAGGCAGCGA GGGTGAGCCC ACCCCAAAGC ATGCCTGAGG CCTGTGCCCG CCTCGCCTGC 
AGCATCTCAG CCCTGCTCCA TGGAGGTGGC CTCGCATGTG AGTGCCACCT GCAGGGTTCA 
CTGAGCACCG AGTGTGCCCC ACTGGGGGGG CAATGCCCCT GCCGCCCTAA TATCACAGGT 
CGTACCTGTG ACCGCTGCTT GCCTGGGACA TTTGGCCTCG GGCCCACCGG GTGCCATGAG 
TGCCGCTGCC ACCCCGAGGG GGCTGCCAGT GCCGTCTGTA ACTCTACGAG TGGGCAGTGC 
ACGTGTTGGG CAGGCTGTGC TGGTCGCCGC TGTGACCGTT GCCTCTCTGG CCGGTGGGGC 
TTCCCACGCT GCCAGCCCTG TGCCTGCAAT GGACACGCCG AGTTGTGCCA CCCACTCACA 
GGCGTCTGCC AGGACTGCCG CGGAGCCACC ACAGGCCGGC ATTGTGAGAG GTGCTTGGAT 
GGCTACTATG GAGACCCCAC CCTGGGCTCA GGCCAGCGGT GCCAGCCCTG CCCCTGTCCT 
GGGCACCCTG GCTCAGGCCT CTATCATGGG ACCTCCTGCC ATGTGGACAG CACCAGTGGA 
CGTGTCCTGT GCCTCTGTGC CCCCGGCTAT GCAGGACCCC GCTGTGACCG CTGCTCCCCC 
GGCTACTTTG GGCGCCCCTG GCCTGGAGAT GACCCCAGAA GGAGTCCCTG CCGGCCCTGC 
CAGTGCAACA ACAATATTGA TCCCCGTGAC CCAGCCGCCT GTGACCCCCA CAGCGGACAT 
TGCCAGCGCT GTCTGCACCA CAGCCACGGC GCTGGCTGTG CCCACTGCCG GCCTGGCTTC 
CACGGCAGTG CCCTGCGCCC AGGGGGCTGC CGGCGCTGCA GCTGTGACCC CCGGGGCACC 
AGCCCTGTAA GGTGCCCACC TGAGGCTGAA GCCTGCTTCT GTGACCCGGT CAGCGGGCAG 
TGCCCCTGCC GCCCCCACAC ACTGGGGCGG GACTGTAGCC GCTGTGCCCC CCTCTTCTGG 
AACCTCGGGG GGCCCCGGGG CTGTGAGCCC TGCAGCTGCC ACGCCCGACA CACTTTGCAG 
CCGGGGTGTC ACCCGGTCAC GGGTCAGTGC CCCTGCCGGG CAGGATTTGG GGGCCGCACG 
TGCTCCCGGT GTCAGGATGG GTACTGGGGT GACCCGGAGC AGGAGTGCAG AGCCTGTGCC 
TGTGACCCCC AGGGCTCCAT CTCGCCCAGC TGTGACCCGC ACACAGGCAC CTGCCGCTGC 
CGAGAGGGTA TCTCAGGGCC GAGGTGCCAG GCGTGTGCCC GTGGCTCCAC AGGAGGCTTC 
CCACACTGCA CGTCCTGCCC TCCCTGCTTC GCCTCCTGGG ACCAACGCCT GGCTCCCCTC 
CAGCTGCATC TGGACACCAT GGTCCATGAG GTGGCTGCCC TGCGCCAGGG CATGCCTGGC 
TGGGGTGCTG GGCTCCGGGG AGATCAGCTG CAGGCCCTGG AGGGTAAACT CCAGCAGGCT 
CAGACACTCC TGGAATCACC TTCCCCCACC AGAGGTCCCC TGCAACAATT TACAGAGTGG 
ATCACCGGAC TCAGGCAAGG GGTCTGGGGT CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324217.1
CDS	1033..5028
Translation	MPDAGSHCPGAPAGLGPLLLALLLLLPPMVPAPRTSAQEVPDMMPPPGCSGGNCHPPTGNLVIGRGQSLRTSSTCGLHGPELYCVVSKLQDSQNCCFCDSRDGKSHGIENVVSRSDPDGRKTWWQAESGVENVTIQLDLEGAFYFTHLIMTFKTFRPAALLLERSVDHGHSWHVFRYFAHNCSGLFPGIPPAPGRRVSDLVCDQRYSDIEPATEGEVIFQVLDPSFLVENTNNAEIQELLRVTNLRVNFSKLHTLGDRPLGGLRGHPFYYYALYELVIAGSCLCHGHASECRPAPGSPPSVEGMVHGHCVCRHHTTGTHCERCQGLYQDHPWQAAEPGYPHTCQECKCHGHARSCHFDMALYLASGNVSGGVCDACQHNTAGRHCELCQPFFHRDPLEDPRSLHSCKPCDCNPMGALEGGLCDAYTDTTRGLLSGQCRCKVHVWGQRCDSCRPGHYGLSLTQSEGCQPCRCNARGRVPSTQACDPSSGACHCKRFVSGRDCSRCLPEFWGLSSDSLGCRPCDCDFGGAYSNRCSAGQGLCLCRPHLHGRHCQELQSGYFCATLDQATAEAEHGRSLQPADPQLPGAPWPAPRYCTPAPGTSSGWLGPRLWRQRDPDCVLQRARQARYSHQLPRTPGKPPPLHQGSGFALGRGSDFARVVDGAGLSLLAPTVPRALDYDIVLRYETQAPEVWQALVRVRAQSQPRSTRCARPLPSEQLFQATLTHARRAVVLSRPFCFEPGTRYSVTLRLWRTKEGRRPEGGTILLDSVVLLPRVGELPGLRSVDPGALRRLQELHEAGCLEAARVSPPQSMPEACARLACSISALLHGGGLACECHLQGSLSTECAPLGGQCPCRPNITGRTCDRCLPGTFGLGPTGCHECRCHPEGAASAVCNSTSGQCTCWAGCAGRRCDRCLSGRWGFPRCQPCACNGHAELCHPLTGVCQDCRGATTGRHCERCLDGYYGDPTLGSGQRCQPCPCPGHPGSGLYHGTSCHVDSTSGRVLCLCAPGYAGPRCDRCSPGYFGRPWPGDDPRRSPCRPCQCNNNIDPRDPAACDPHSGHCQRCLHHSHGAGCAHCRPGFHGSALRPGGCRRCSCDPRGTSPVRCPPEAEACFCDPVSGQCPCRPHTLGRDCSRCAPLFWNLGGPRGCEPCSCHARHTLQPGCHPVTGQCPCRAGFGGRTCSRCQDGYWGDPEQECRACACDPQGSISPSCDPHTGTCRCREGISGPRCQACARGSTGGFPHCTSCPPCFASWDQRLAPLQLHLDTMVHEVAALRQGMPGWGAGLRGDQLQALEGKLQQAQTLLESPSPTRGPLQQFTEWITGLRQGVWGH

Target ORF information:

RefSeq Version	XM_015468731.1
Organism	Bos taurus(cattle)
Definition	Bos taurus laminin subunit beta-2 (LOC530599), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468731.1

ORF Insert Sequence:

ATGCCTGATG CTGGCTCCCA CTGCCCAGGA GCCCCAGCCG GGCTAGGCCC CCTTCTCTTA 
GCCCTGCTGC TGCTGCTGCC ACCCATGGTC CCGGCTCCCA GAACAAGTGC CCAGGAGGTA 
CCAGACATGA TGCCTCCTCC CGGCTGCTCA GGTGGCAACT GCCACCCACC TACTGGCAAC 
CTGGTTATTG GCCGTGGCCA GAGCCTTCGA ACCTCATCTA CCTGTGGCCT CCATGGCCCT 
GAACTCTACT GTGTTGTCAG TAAACTACAG GACTCTCAGA ATTGCTGCTT TTGTGATTCT 
CGGGATGGGA AAAGCCATGG CATTGAGAAT GTGGTCTCCC GGAGTGACCC TGATGGCAGA 
AAGACCTGGT GGCAAGCAGA GAGTGGTGTT GAGAATGTCA CCATCCAGTT GGACCTAGAA 
GGTGCCTTTT ACTTTACCCA CCTCATCATG ACTTTTAAGA CATTCCGCCC AGCAGCTCTG 
CTCCTTGAGC GCTCAGTGGA CCATGGCCAC TCCTGGCATG TGTTCCGCTA CTTTGCCCAC 
AACTGCTCAG GCCTCTTTCC TGGGATCCCT CCTGCCCCTG GCCGCAGAGT CAGTGACCTC 
GTCTGTGACC AGCGTTACTC AGACATCGAG CCTGCCACTG AGGGAGAGGT AATTTTCCAA 
GTTCTGGACC CATCCTTTCT GGTGGAGAAC ACAAACAACG CGGAGATTCA GGAACTCCTG 
CGTGTGACTA ACCTCCGTGT GAACTTCTCC AAGCTGCACA CACTGGGTGA CAGGCCCCTG 
GGGGGCCTCA GGGGCCACCC CTTCTACTAC TATGCTCTCT ATGAGCTAGT GATTGCAGGC 
AGCTGCCTGT GCCACGGCCA TGCTTCCGAG TGCAGGCCCG CACCCGGGTC CCCACCCAGC 
GTGGAAGGCA TGGTGCATGG CCATTGTGTC TGCCGCCACC ACACCACTGG TACCCACTGT 
GAACGTTGCC AGGGCCTGTA CCAGGACCAC CCGTGGCAGG CAGCAGAGCC TGGGTACCCC 
CACACCTGCC AGGAATGCAA GTGCCATGGG CACGCCCGCA GTTGTCACTT TGACATGGCC 
CTGTATCTCG CATCTGGCAA CGTGAGTGGA GGTGTGTGCG ATGCATGTCA ACACAACACG 
GCCGGGCGCC ACTGTGAGCT CTGCCAGCCC TTCTTCCACC GGGACCCCCT GGAGGACCCC 
CGCTCCCTTC ACTCCTGCAA ACCCTGTGAC TGCAACCCTA TGGGTGCCCT GGAAGGGGGT 
TTGTGTGATG CCTACACAGA CACCACCCGG GGTCTCCTCT CTGGGCAGTG TCGCTGCAAA 
GTTCACGTCT GGGGCCAGCG CTGTGACTCC TGCCGGCCAG GTCACTATGG CCTGAGCCTC 
ACCCAGTCAG AAGGCTGCCA GCCCTGCAGG TGCAACGCCC GGGGCCGGGT CCCCAGCACT 
CAGGCCTGTG ATCCATCCAG CGGTGCCTGT CACTGCAAAC GCTTTGTCTC TGGACGGGAC 
TGCAGCCGCT GTCTGCCTGA GTTCTGGGGT CTGAGCAGTG ACTCTCTAGG CTGTCGGCCC 
TGTGACTGTG ACTTTGGGGG TGCCTACAGC AACAGGTGTT CTGCAGGGCA GGGCCTCTGC 
CTCTGCCGCC CCCACCTGCA TGGCCGCCAC TGCCAGGAAC TCCAGTCTGG GTACTTCTGC 
GCCACCCTCG ACCAGGCCAC TGCCGAAGCA GAGCATGGCC GCAGCCTCCA GCCTGCTGAC 
CCCCAGCTGC CTGGAGCCCC CTGGCCTGCT CCCCGCTACT GTACACCAGC CCCAGGGACC 
TCCTCTGGTT GGCTCGGGCC CCGACTTTGG AGACAGCGTG ACCCTGACTG TGTCCTCCAA 
CGTGCCAGGC AAGCCCGGTA CAGCCACCAG CTGCCCAGGA CCCCAGGGAA GCCCCCGCCC 
CTGCATCAGG GATCTGGCTT TGCACTGGGG AGGGGCTCAG ACTTCGCACG TGTGGTGGAC 
GGGGCTGGCC TCTCTCTGCT GGCACCCACA GTGCCTCGCG CCCTGGACTA TGACATCGTC 
CTACGCTATG AGACCCAGGC ACCTGAAGTG TGGCAGGCAT TAGTCCGGGT CCGTGCCCAG 
TCACAGCCCC GCAGTACCCG CTGTGCCCGT CCGTTGCCCT CAGAGCAGCT GTTCCAGGCG 
ACCTTGACCC ATGCGCGCAG AGCCGTGGTT CTTTCCAGAC CGTTCTGCTT TGAACCCGGA 
ACCCGCTACT CCGTGACCCT GCGGCTGTGG CGGACCAAGG AGGGACGGAG ACCCGAAGGG 
GGCACGATCC TCCTGGATTC AGTAGTGCTC CTGCCGCGGG TTGGAGAGTT GCCTGGACTG 
AGGTCCGTGG ACCCGGGGGC CCTGAGGCGC TTGCAGGAGC TCCACGAGGC GGGCTGCCTG 
GAGGCAGCGA GGGTGAGCCC ACCCCAAAGC ATGCCTGAGG CCTGTGCCCG CCTCGCCTGC 
AGCATCTCAG CCCTGCTCCA TGGAGGTGGC CTCGCATGTG AGTGCCACCT GCAGGGTTCA 
CTGAGCACCG AGTGTGCCCC ACTGGGGGGG CAATGCCCCT GCCGCCCTAA TATCACAGGT 
CGTACCTGTG ACCGCTGCTT GCCTGGGACA TTTGGCCTCG GGCCCACCGG GTGCCATGAG 
TGCCGCTGCC ACCCCGAGGG GGCTGCCAGT GCCGTCTGTA ACTCTACGAG TGGGCAGTGC 
ACGTGTTGGG CAGGCTGTGC TGGTCGCCGC TGTGACCGTT GCCTCTCTGG CCGGTGGGGC 
TTCCCACGCT GCCAGCCCTG TGCCTGCAAT GGACACGCCG AGTTGTGCCA CCCACTCACA 
GGCGTCTGCC AGGACTGCCG CGGAGCCACC ACAGGCCGGC ATTGTGAGAG GTGCTTGGAT 
GGCTACTATG GAGACCCCAC CCTGGGCTCA GGCCAGCGGT GCCAGCCCTG CCCCTGTCCT 
GGGCACCCTG GCTCAGGCCT CTATCATGGG ACCTCCTGCC ATGTGGACAG CACCAGTGGA 
CGTGTCCTGT GCCTCTGTGC CCCCGGCTAT GCAGGACCCC GCTGTGACCG CTGCTCCCCC 
GGCTACTTTG GGCGCCCCTG GCCTGGAGAT GACCCCAGAA GGAGTCCCTG CCGGCCCTGC 
CAGTGCAACA ACAATATTGA TCCCCGTGAC CCAGCCGCCT GTGACCCCCA CAGCGGACAT 
TGCCAGCGCT GTCTGCACCA CAGCCACGGC GCTGGCTGTG CCCACTGCCG GCCTGGCTTC 
CACGGCAGTG CCCTGCGCCC AGGGGGCTGC CGGCGCTGCA GCTGTGACCC CCGGGGCACC 
AGCCCTGTAA GGTGCCCACC TGAGGCTGAA GCCTGCTTCT GTGACCCGGT CAGCGGGCAG 
TGCCCCTGCC GCCCCCACAC ACTGGGGCGG GACTGTAGCC GCTGTGCCCC CCTCTTCTGG 
AACCTCGGGG GGCCCCGGGG CTGTGAGCCC TGCAGCTGCC ACGCCCGACA CACTTTGCAG 
CCGGGGTGTC ACCCGGTCAC GGGTCAGTGC CCCTGCCGGG CAGGATTTGG GGGCCGCACG 
TGCTCCCGGT GTCAGGATGG GTACTGGGGT GACCCGGAGC AGGAGTGCAG AGCCTGTGCC 
TGTGACCCCC AGGGCTCCAT CTCGCCCAGC TGTGACCCGC ACACAGGCAC CTGCCGCTGC 
CGAGAGGGTA TCTCAGGGCC GAGGTGCCAG GCGTGTGCCC GTGGCTCCAC AGGAGGCTTC 
CCACACTGCA CGTCCTGCCC TCCCTGCTTC GCCTCCTGGG ACCAACGCCT GGCTCCCCTC 
CAGCTGCATC TGGACACCAT GGTCCATGAG GTGGCTGCCC TGCGCCAGGG CATGCCTGGC 
TGGGGTGCTG GGCTCCGGGG AGATCAGCTG CAGGCCCTGG AGGGTAAACT CCAGCAGGCT 
CAGACACTCC TGGAATCACC TTCCCCCACC AGAGGTCCCC TGCAACAATT TACAGAGTGG 
ATCACCGGAC TCAGGCAAGG GGTCTGGGGT CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC530599 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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