GRK6 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GRK6
Entrez Gene ID	521413
Full Name	G protein-coupled receptor kinase 6
General protein information	Preferred Names G protein-coupled receptor kinase 6 Names G protein-coupled receptor kinase 6
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024994253.1	XP_024850021.1	G protein-coupled receptor kinase 6 isoform X2
XM_024994254.1	XP_024850022.1	G protein-coupled receptor kinase 6 isoform X2
NM_001205390.1	NP_001192319.1	G protein-coupled receptor kinase 6 isoform 2
XM_024994251.1	XP_024850019.1	G protein-coupled receptor kinase 6 isoform X1
XM_015472157.1	XP_015327643.1	G protein-coupled receptor kinase 6 isoform X1
XM_010807152.2	XP_010805454.1	G protein-coupled receptor kinase 6 isoform X2
NM_001191530.1	NP_001178459.1	G protein-coupled receptor kinase 6 isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GRK6	XP_003434543.2
Mus musculus (house mouse)	Grk6	NP_001033107.1
Rattus norvegicus (Norway rat)	Grk6	NP_113845.2
Gallus gallus (chicken)	GRK6	XP_414676.3
Danio rerio (zebrafish)	grk6	XP_689783.2
Xenopus tropicalis (tropical clawed frog)	grk6	XP_002936988.1
Pan troglodytes (chimpanzee)	GRK6	XP_003311032.1
	GRK6	XP_001090003.2
Bos taurus (cattle)	GRK6	NP_001178459.1
Homo sapiens (human)	GRK6	NP_002073.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
Jann OC, Aerts J, Jones M, Hastings N, Law A, McKay S, Marques E, Prasad A, Yu J, Moore SS, Floriot S, Mah? MF, Eggen A, Silveri L, Negrini R, Milanesi E, Ajmone-Marsan P, Valentini A, Marchitelli C, Savarese MC, Janitz M, Herwig R, Hennig S, Gorni C, Connor EE, Sonstegard TS, Smith T, Dr?gem?ller C, Williams JL
BMC genomics7283(2006 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GRK6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRK6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa37212	NM_001205390.1 Latest version!	Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa50397	XM_010807152.2 Latest version!	Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa236045	XM_024994254.1 Latest version!	Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa236045	XM_024994253.1 Latest version!	Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa236044	XM_024994251.1 Latest version!	Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa37060	NM_001191530.1 Latest version!	Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OBa91111	XM_015472157.1 Latest version!	Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GRK6 ( NM_001205390.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192319.1 Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OBa37212
Clone ID Related Accession (Same CDS sequence)	NM_001205390.1
Accession Version	NM_001205390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Bos taurus(cattle)
Product	G protein-coupled receptor kinase 6 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000007.1, DY071905.1, BF605412.1, CO873051.1, BE664831.1, EV633678.1, DY158204.1 and CO876214.1. On or before Apr 21, 2011 this sequence version replaced XM_002689020.1, XM_002689021.1. Transcript Variant: This variant (2) lacks an alternate short exon in the 3' coding region resulting in a frameshift, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571254.12594.1, SRR5571261.7286.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCTCG AGAACATCGT AGCGAACACG GTGCTACTCA AGGCCCGGGA AGGTGGTGGC 
GGGAATCGTA AAGGCAAAAG CAAGAAATGG CGGCAGATGC TACAGTTCCC CCATATCAGC 
CAGTGTGAAG AGCTACGGCT CAGCCTCGAG CGTGACTACC ACAGCCTGTG TGAGCGGCAG 
CCCATTGGGC GCCTGCTGTT CCGAGAGTTC TGCGCCACGA GGCCTGAGCT GACTCGCTGC 
ATTGCCTTCC TGGACGGGGT GGCCGAGTAT GAAGTGACCC CTGATGAGAA GCGGAAGGCA 
TGTGGGCTGC GGCTCATGCA GAATTTTCTG AGCCACACGG GTCCTGACCT CATCCCCGAG 
GTCCCCCGGC AACTGGTGAC GAACTGTGCC CAGCGGCTGG AGGAAGGACC CTGCAAAGAC 
CTCTTCCAGG AGCTTACTCG GTTGACCCAT GAGTACCTGA GCATGGCCCC TTTTGCCGAC 
TACCTCGACA GCATCTACTT CAACCGTTTT CTGCAGTGGA AGTGGCTGGA AAGGCAGCCA 
GTGACCAAAA ACACCTTCAG GCAGTACCGA GTCCTGGGCA AAGGCGGCTT TGGGGAGGTA 
TGCGCCTGCC AGGTGCGGGC CACAGGCAAG ATGTACGCCT GCAAGAAGCT GGAGAAGAAG 
CGGATCAAGA AGCGGAAAGG GGAAGCCATG GCACTCAACG AGAAGCAGAT TCTGGAGAAA 
GTGAACAGTA GGTTTGTAGT GAGCTTGGCC TACGCCTATG AGACCAAGGA TGCACTGTGC 
CTGGTGCTGA CGCTGATGAA CGGAGGCGAC CTCAAGTTCC ACATCTACCA CATGGGCCAG 
GCTGGCTTCC CTGAGGCTCG GGCTGTCTTC TACGCAGCAG AGATCTGCTG TGGCCTGGAG 
GACCTGCACC GGGAGCGTAT CGTGTACAGG GACCTAAAGC CAGAGAACAT CCTACTAGAT 
GACCATGGTC ACATCCGCAT CTCTGACCTG GGGCTGGCTG TGCACGTACC CGAAGGCCAG 
ACGATCAAAG GCCGTGTGGG CACCGTGGGC TACATGGCCC CAGAGGTGGT AAAAAACGAA 
CGGTACACCT TTAGCCCGGA CTGGTGGGCA CTCGGCTGCC TCCTGTATGA GATGATCGCA 
GGCCAGTCAC CCTTCCAGCA GAGGAAAAAG AAGATCAAGC GGGAGGAAGT GGAGCGGCTG 
GTGAAGGAGG TGCCTGAGGA GTACTCCGAG CACTTCTCCC CACAGGCCCG CTCACTCTGC 
ACCCAGCTCC TCTGCAAGGA CCCCTCCGAA CGCCTGGGGT GTGGTGGGGG CGGCGCGCAA 
GAAGTGAAGG AGCACCCCCT CTTCAAGAAG CTGAACTTCA AGCGGCTGGG AGCTGGCATG 
CTGGAACCAC CCTTCAAGCC TGATCCCCAG GCCATTTACT GCAAAGATGT TCTGGACATT 
GAACAGTTCT CAACAGTTAA GGGTGTGGAG CTAGAGCCCA CTGACCAGGA CTTCTACCAG 
AAATTTGCCA CAGGCAGTGT GCCCATCCCT TGGCAGAACG AGATGGTGGA GACCGAGTGC 
TTCCAGGAGC TGAACGTCTT CGGGCTGGAT GGCTCAGTTC CCCCAGACCT AGACTGGAAG 
GGCCAGCCAC CAGCACCCCC CAAAAAGGGA CTGCTGCAGA GACTCTTCAG TCGCCAGGAC 
TGCTGTGGAA ACTGCAGCGA CAGTGAGGAA GAGCTTCCCG CCCGCCTCGA GCTCCCCACC 
CGCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192319.1
CDS	101..1849
Translation	MELENIVANTVLLKAREGGGGNRKGKSKKWRQMLQFPHISQCEELRLSLERDYHSLCERQPIGRLLFREFCATRPELTRCIAFLDGVAEYEVTPDEKRKACGLRLMQNFLSHTGPDLIPEVPRQLVTNCAQRLEEGPCKDLFQELTRLTHEYLSMAPFADYLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYHMGQAGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVGYMAPEVVKNERYTFSPDWWALGCLLYEMIAGQSPFQQRKKKIKREEVERLVKEVPEEYSEHFSPQARSLCTQLLCKDPSERLGCGGGGAQEVKEHPLFKKLNFKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVELEPTDQDFYQKFATGSVPIPWQNEMVETECFQELNVFGLDGSVPPDLDWKGQPPAPPKKGLLQRLFSRQDCCGNCSDSEEELPARLELPTRL

Target ORF information:

RefSeq Version	NM_001205390.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205390.1

ORF Insert Sequence:

ATGGAGCTCG AGAACATCGT AGCGAACACG GTGCTACTCA AGGCCCGGGA AGGTGGTGGC 
GGGAATCGTA AAGGCAAAAG CAAGAAATGG CGGCAGATGC TACAGTTCCC CCATATCAGC 
CAGTGTGAAG AGCTACGGCT CAGCCTCGAG CGTGACTACC ACAGCCTGTG TGAGCGGCAG 
CCCATTGGGC GCCTGCTGTT CCGAGAGTTC TGCGCCACGA GGCCTGAGCT GACTCGCTGC 
ATTGCCTTCC TGGACGGGGT GGCCGAGTAT GAAGTGACCC CTGATGAGAA GCGGAAGGCA 
TGTGGGCTGC GGCTCATGCA GAATTTTCTG AGCCACACGG GTCCTGACCT CATCCCCGAG 
GTCCCCCGGC AACTGGTGAC GAACTGTGCC CAGCGGCTGG AGGAAGGACC CTGCAAAGAC 
CTCTTCCAGG AGCTTACTCG GTTGACCCAT GAGTACCTGA GCATGGCCCC TTTTGCCGAC 
TACCTCGACA GCATCTACTT CAACCGTTTT CTGCAGTGGA AGTGGCTGGA AAGGCAGCCA 
GTGACCAAAA ACACCTTCAG GCAGTACCGA GTCCTGGGCA AAGGCGGCTT TGGGGAGGTA 
TGCGCCTGCC AGGTGCGGGC CACAGGCAAG ATGTACGCCT GCAAGAAGCT GGAGAAGAAG 
CGGATCAAGA AGCGGAAAGG GGAAGCCATG GCACTCAACG AGAAGCAGAT TCTGGAGAAA 
GTGAACAGTA GGTTTGTAGT GAGCTTGGCC TACGCCTATG AGACCAAGGA TGCACTGTGC 
CTGGTGCTGA CGCTGATGAA CGGAGGCGAC CTCAAGTTCC ACATCTACCA CATGGGCCAG 
GCTGGCTTCC CTGAGGCTCG GGCTGTCTTC TACGCAGCAG AGATCTGCTG TGGCCTGGAG 
GACCTGCACC GGGAGCGTAT CGTGTACAGG GACCTAAAGC CAGAGAACAT CCTACTAGAT 
GACCATGGTC ACATCCGCAT CTCTGACCTG GGGCTGGCTG TGCACGTACC CGAAGGCCAG 
ACGATCAAAG GCCGTGTGGG CACCGTGGGC TACATGGCCC CAGAGGTGGT AAAAAACGAA 
CGGTACACCT TTAGCCCGGA CTGGTGGGCA CTCGGCTGCC TCCTGTATGA GATGATCGCA 
GGCCAGTCAC CCTTCCAGCA GAGGAAAAAG AAGATCAAGC GGGAGGAAGT GGAGCGGCTG 
GTGAAGGAGG TGCCTGAGGA GTACTCCGAG CACTTCTCCC CACAGGCCCG CTCACTCTGC 
ACCCAGCTCC TCTGCAAGGA CCCCTCCGAA CGCCTGGGGT GTGGTGGGGG CGGCGCGCAA 
GAAGTGAAGG AGCACCCCCT CTTCAAGAAG CTGAACTTCA AGCGGCTGGG AGCTGGCATG 
CTGGAACCAC CCTTCAAGCC TGATCCCCAG GCCATTTACT GCAAAGATGT TCTGGACATT 
GAACAGTTCT CAACAGTTAA GGGTGTGGAG CTAGAGCCCA CTGACCAGGA CTTCTACCAG 
AAATTTGCCA CAGGCAGTGT GCCCATCCCT TGGCAGAACG AGATGGTGGA GACCGAGTGC 
TTCCAGGAGC TGAACGTCTT CGGGCTGGAT GGCTCAGTTC CCCCAGACCT AGACTGGAAG 
GGCCAGCCAC CAGCACCCCC CAAAAAGGGA CTGCTGCAGA GACTCTTCAG TCGCCAGGAC 
TGCTGTGGAA ACTGCAGCGA CAGTGAGGAA GAGCTTCCCG CCCGCCTCGA GCTCCCCACC 
CGCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK6 ( XM_010807152.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805454.1 Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa50397
Clone ID Related Accession (Same CDS sequence)	XM_010807152.2
Accession Version	XM_010807152.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G protein-coupled receptor kinase 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807152.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCG AGAACATCGT AGCGAACACG GTGCTACTCA AGGCCCGGGA AGGTGGTGGC 
GGGAATCGTA AAGGCAAAAG CAAGAAATGG CGGCAGATGC TACAGTTCCC CCATATCAGC 
CAGTGTGAAG AGCTACGGCT CAGCCTCGAG CGTGACTACC ACAGCCTGTG TGAGCGGCAG 
CCCATTGGGC GCCTGCTGTT CCGAGAGTTC TGCGCCACGA GGCCTGAGCT GACTCGCTGC 
ATTGCCTTCC TGGACGGGGT GGCCGAGTAT GAAGTGACCC CTGATGAGAA GCGGAAGGCA 
TGTGGGCTGC GGCTCATGCA GAATTTTCTG AGCCACACGG GTCCTGACCT CATCCCCGAG 
GTCCCCCGGC AACTGGTGAC GAACTGTGCC CAGCGGCTGG AGGAAGGACC CTGCAAAGAC 
CTCTTCCAGG AGCTTACTCG GTTGACCCAT GAGTACCTGA GCATGGCCCC TTTTGCCGAC 
TACCTCGACA GCATCTACTT CAACCGTTTT CTGCAGTGGA AGTGGCTGGA AAGGCAGCCA 
GTGACCAAAA ACACCTTCAG GCAGTACCGA GTCCTGGGCA AAGGCGGCTT TGGGGAGGTG 
AGTATGCGCT GCCAGGTGCG GGCCACAGGC AAGATGTACG CCTGCAAGAA GCTGGAGAAG 
AAGCGGATCA AGAAGCGGAA AGGGGAAGCC ATGGCACTCA ACGAGAAGCA GATTCTGGAG 
AAAGTGAACA GTAGGTTTGT AGTGAGCTTG GCCTACGCCT ATGAGACCAA GGATGCACTG 
TGCCTGGTGC TGACGCTGAT GAACGGAGGC GACCTCAAGT TCCACATCTA CCACATGGGC 
CAGGCTGGCT TCCCTGAGGC TCGGGCTGTC TTCTACGCAG CAGAGATCTG CTGTGGCCTG 
GAGGACCTGC ACCGGGAGCG TATCGTGTAC AGGGACCTAA AGCCAGAGAA CATCCTACTA 
GATGACCATG GTCACATCCG CATCTCTGAC CTGGGGCTGG CTGTGCACGT ACCCGAAGGC 
CAGACGATCA AAGGCCGTGT GGGCACCGTG GGCTACATGG CCCCAGAGGT GGTAAAAAAC 
GAACGGTACA CCTTTAGCCC GGACTGGTGG GCACTCGGCT GCCTCCTGTA TGAGATGATC 
GCAGGCCAGT CACCCTTCCA GCAGAGGAAA AAGAAGATCA AGCGGGAGGA AGTGGAGCGG 
CTGGTGAAGG AGGTGCCTGA GGAGTACTCC GAGCACTTCT CCCCACAGGC CCGCTCACTC 
TGCACCCAGC TCCTCTGCAA GGACCCCTCC GAACGCCTGG GGTGTGGTGG GGGCGGCGCG 
CAAGAAGTGA AGGAGCACCC CCTCTTCAAG AAGCTGAACT TCAAGCGGCT GGGAGCTGGC 
ATGCTGGAAC CACCCTTCAA GCCTGATCCC CAGGCCATTT ACTGCAAAGA TGTTCTGGAC 
ATTGAACAGT TCTCAACAGT TAAGGGTGTG GAGCTAGAGC CCACTGACCA GGACTTCTAC 
CAGAAATTTG CCACAGGCAG TGTGCCCATC CCTTGGCAGA ACGAGATGGT GGAGACCGAG 
TGCTTCCAGG AGCTGAACGT CTTCGGGCTG GATGGCTCAG TTCCCCCAGA CCTAGACTGG 
AAGGGCCAGC CACCAGCACC CCCCAAAAAG GGACTGCTGC AGAGACTCTT CAGTCGCCAG 
GACTGCTGTG GAAACTGCAG CGACAGTGAG GAAGAGCTTC CCGCCCGCCT CGAGCTCCCC 
ACCCGCCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805454.1
CDS	143..1894
Translation	MELENIVANTVLLKAREGGGGNRKGKSKKWRQMLQFPHISQCEELRLSLERDYHSLCERQPIGRLLFREFCATRPELTRCIAFLDGVAEYEVTPDEKRKACGLRLMQNFLSHTGPDLIPEVPRQLVTNCAQRLEEGPCKDLFQELTRLTHEYLSMAPFADYLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVSMRCQVRATGKMYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYHMGQAGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVGYMAPEVVKNERYTFSPDWWALGCLLYEMIAGQSPFQQRKKKIKREEVERLVKEVPEEYSEHFSPQARSLCTQLLCKDPSERLGCGGGGAQEVKEHPLFKKLNFKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVELEPTDQDFYQKFATGSVPIPWQNEMVETECFQELNVFGLDGSVPPDLDWKGQPPAPPKKGLLQRLFSRQDCCGNCSDSEEELPARLELPTRL

Target ORF information:

RefSeq Version	XM_010807152.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807152.2

ORF Insert Sequence:

ATGGAGCTCG AGAACATCGT AGCGAACACG GTGCTACTCA AGGCCCGGGA AGGTGGTGGC 
GGGAATCGTA AAGGCAAAAG CAAGAAATGG CGGCAGATGC TACAGTTCCC CCATATCAGC 
CAGTGTGAAG AGCTACGGCT CAGCCTCGAG CGTGACTACC ACAGCCTGTG TGAGCGGCAG 
CCCATTGGGC GCCTGCTGTT CCGAGAGTTC TGCGCCACGA GGCCTGAGCT GACTCGCTGC 
ATTGCCTTCC TGGACGGGGT GGCCGAGTAT GAAGTGACCC CTGATGAGAA GCGGAAGGCA 
TGTGGGCTGC GGCTCATGCA GAATTTTCTG AGCCACACGG GTCCTGACCT CATCCCCGAG 
GTCCCCCGGC AACTGGTGAC GAACTGTGCC CAGCGGCTGG AGGAAGGACC CTGCAAAGAC 
CTCTTCCAGG AGCTTACTCG GTTGACCCAT GAGTACCTGA GCATGGCCCC TTTTGCCGAC 
TACCTCGACA GCATCTACTT CAACCGTTTT CTGCAGTGGA AGTGGCTGGA AAGGCAGCCA 
GTGACCAAAA ACACCTTCAG GCAGTACCGA GTCCTGGGCA AAGGCGGCTT TGGGGAGGTG 
AGTATGCGCT GCCAGGTGCG GGCCACAGGC AAGATGTACG CCTGCAAGAA GCTGGAGAAG 
AAGCGGATCA AGAAGCGGAA AGGGGAAGCC ATGGCACTCA ACGAGAAGCA GATTCTGGAG 
AAAGTGAACA GTAGGTTTGT AGTGAGCTTG GCCTACGCCT ATGAGACCAA GGATGCACTG 
TGCCTGGTGC TGACGCTGAT GAACGGAGGC GACCTCAAGT TCCACATCTA CCACATGGGC 
CAGGCTGGCT TCCCTGAGGC TCGGGCTGTC TTCTACGCAG CAGAGATCTG CTGTGGCCTG 
GAGGACCTGC ACCGGGAGCG TATCGTGTAC AGGGACCTAA AGCCAGAGAA CATCCTACTA 
GATGACCATG GTCACATCCG CATCTCTGAC CTGGGGCTGG CTGTGCACGT ACCCGAAGGC 
CAGACGATCA AAGGCCGTGT GGGCACCGTG GGCTACATGG CCCCAGAGGT GGTAAAAAAC 
GAACGGTACA CCTTTAGCCC GGACTGGTGG GCACTCGGCT GCCTCCTGTA TGAGATGATC 
GCAGGCCAGT CACCCTTCCA GCAGAGGAAA AAGAAGATCA AGCGGGAGGA AGTGGAGCGG 
CTGGTGAAGG AGGTGCCTGA GGAGTACTCC GAGCACTTCT CCCCACAGGC CCGCTCACTC 
TGCACCCAGC TCCTCTGCAA GGACCCCTCC GAACGCCTGG GGTGTGGTGG GGGCGGCGCG 
CAAGAAGTGA AGGAGCACCC CCTCTTCAAG AAGCTGAACT TCAAGCGGCT GGGAGCTGGC 
ATGCTGGAAC CACCCTTCAA GCCTGATCCC CAGGCCATTT ACTGCAAAGA TGTTCTGGAC 
ATTGAACAGT TCTCAACAGT TAAGGGTGTG GAGCTAGAGC CCACTGACCA GGACTTCTAC 
CAGAAATTTG CCACAGGCAG TGTGCCCATC CCTTGGCAGA ACGAGATGGT GGAGACCGAG 
TGCTTCCAGG AGCTGAACGT CTTCGGGCTG GATGGCTCAG TTCCCCCAGA CCTAGACTGG 
AAGGGCCAGC CACCAGCACC CCCCAAAAAG GGACTGCTGC AGAGACTCTT CAGTCGCCAG 
GACTGCTGTG GAAACTGCAG CGACAGTGAG GAAGAGCTTC CCGCCCGCCT CGAGCTCCCC 
ACCCGCCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK6 ( XM_024994253.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850021.1 Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa236045
Clone ID Related Accession (Same CDS sequence)	XM_024994253.1 , XM_024994254.1
Accession Version	XM_024994253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	G protein-coupled receptor kinase 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATT TTCTGAGCCA CACGGGTCCT GACCTCATCC CCGAGGTCCC CCGGCAACTG 
GTGACGAACT GTGCCCAGCG GCTGGAGGAA GGACCCTGCA AAGACCTCTT CCAGGAGCTT 
ACTCGGTTGA CCCATGAGTA CCTGAGCATG GCCCCTTTTG CCGACTACCT CGACAGCATC 
TACTTCAACC GTTTTCTGCA GTGGAAGTGG CTGGAAAGGC AGCCAGTGAC CAAAAACACC 
TTCAGGCAGT ACCGAGTCCT GGGCAAAGGC GGCTTTGGGG AGGTATGCGC CTGCCAGGTG 
CGGGCCACAG GCAAGATGTA CGCCTGCAAG AAGCTGGAGA AGAAGCGGAT CAAGAAGCGG 
AAAGGGGAAG CCATGGCACT CAACGAGAAG CAGATTCTGG AGAAAGTGAA CAGTAGGTTT 
GTAGTGAGCT TGGCCTACGC CTATGAGACC AAGGATGCAC TGTGCCTGGT GCTGACGCTG 
ATGAACGGAG GCGACCTCAA GTTCCACATC TACCACATGG GCCAGGCTGG CTTCCCTGAG 
GCTCGGGCTG TCTTCTACGC AGCAGAGATC TGCTGTGGCC TGGAGGACCT GCACCGGGAG 
CGTATCGTGT ACAGGGACCT AAAGCCAGAG AACATCCTAC TAGATGACCA TGGTCACATC 
CGCATCTCTG ACCTGGGGCT GGCTGTGCAC GTACCCGAAG GCCAGACGAT CAAAGGCCGT 
GTGGGCACCG TGGGCTACAT GGCCCCAGAG GTGGTAAAAA ACGAACGGTA CACCTTTAGC 
CCGGACTGGT GGGCACTCGG CTGCCTCCTG TATGAGATGA TCGCAGGCCA GTCACCCTTC 
CAGCAGAGGA AAAAGAAGAT CAAGCGGGAG GAAGTGGAGC GGCTGGTGAA GGAGGTGCCT 
GAGGAGTACT CCGAGCACTT CTCCCCACAG GCCCGCTCAC TCTGCACCCA GCTCCTCTGC 
AAGGACCCCT CCGAACGCCT GGGGTGTGGT GGGGGCGGCG CGCAAGAAGT GAAGGAGCAC 
CCCCTCTTCA AGAAGCTGAA CTTCAAGCGG CTGGGAGCTG GCATGCTGGA ACCACCCTTC 
AAGCCTGATC CCCAGGCCAT TTACTGCAAA GATGTTCTGG ACATTGAACA GTTCTCAACA 
GTTAAGGGTG TGGAGCTAGA GCCCACTGAC CAGGACTTCT ACCAGAAATT TGCCACAGGC 
AGTGTGCCCA TCCCTTGGCA GAACGAGATG GTGGAGACCG AGTGCTTCCA GGAGCTGAAC 
GTCTTCGGGC TGGATGGCTC AGTTCCCCCA GACCTAGACT GGAAGGGCCA GCCACCAGCA 
CCCCCCAAAA AGGGACTGCT GCAGAGACTC TTCAGTCGCC AGAGGACTGC TGTGGAAACT 
GCAGCGACAG TGAGGAAGAG CTTCCCGCCC GCCTCGAGCT CCCCACCCGC CTCTAGCCCC 
CAGCCTGGGG CCCCCCCGGG CGGTTGGCGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850021.1
CDS	370..1842
Translation	MQNFLSHTGPDLIPEVPRQLVTNCAQRLEEGPCKDLFQELTRLTHEYLSMAPFADYLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYHMGQAGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVGYMAPEVVKNERYTFSPDWWALGCLLYEMIAGQSPFQQRKKKIKREEVERLVKEVPEEYSEHFSPQARSLCTQLLCKDPSERLGCGGGGAQEVKEHPLFKKLNFKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVELEPTDQDFYQKFATGSVPIPWQNEMVETECFQELNVFGLDGSVPPDLDWKGQPPAPPKKGLLQRLFSRQRTAVETAATVRKSFPPASSSPPASSPQPGAPPGGWR

Target ORF information:

RefSeq Version	XM_024994253.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994253.1

ORF Insert Sequence:

ATGCAGAATT TTCTGAGCCA CACGGGTCCT GACCTCATCC CCGAGGTCCC CCGGCAACTG 
GTGACGAACT GTGCCCAGCG GCTGGAGGAA GGACCCTGCA AAGACCTCTT CCAGGAGCTT 
ACTCGGTTGA CCCATGAGTA CCTGAGCATG GCCCCTTTTG CCGACTACCT CGACAGCATC 
TACTTCAACC GTTTTCTGCA GTGGAAGTGG CTGGAAAGGC AGCCAGTGAC CAAAAACACC 
TTCAGGCAGT ACCGAGTCCT GGGCAAAGGC GGCTTTGGGG AGGTATGCGC CTGCCAGGTG 
CGGGCCACAG GCAAGATGTA CGCCTGCAAG AAGCTGGAGA AGAAGCGGAT CAAGAAGCGG 
AAAGGGGAAG CCATGGCACT CAACGAGAAG CAGATTCTGG AGAAAGTGAA CAGTAGGTTT 
GTAGTGAGCT TGGCCTACGC CTATGAGACC AAGGATGCAC TGTGCCTGGT GCTGACGCTG 
ATGAACGGAG GCGACCTCAA GTTCCACATC TACCACATGG GCCAGGCTGG CTTCCCTGAG 
GCTCGGGCTG TCTTCTACGC AGCAGAGATC TGCTGTGGCC TGGAGGACCT GCACCGGGAG 
CGTATCGTGT ACAGGGACCT AAAGCCAGAG AACATCCTAC TAGATGACCA TGGTCACATC 
CGCATCTCTG ACCTGGGGCT GGCTGTGCAC GTACCCGAAG GCCAGACGAT CAAAGGCCGT 
GTGGGCACCG TGGGCTACAT GGCCCCAGAG GTGGTAAAAA ACGAACGGTA CACCTTTAGC 
CCGGACTGGT GGGCACTCGG CTGCCTCCTG TATGAGATGA TCGCAGGCCA GTCACCCTTC 
CAGCAGAGGA AAAAGAAGAT CAAGCGGGAG GAAGTGGAGC GGCTGGTGAA GGAGGTGCCT 
GAGGAGTACT CCGAGCACTT CTCCCCACAG GCCCGCTCAC TCTGCACCCA GCTCCTCTGC 
AAGGACCCCT CCGAACGCCT GGGGTGTGGT GGGGGCGGCG CGCAAGAAGT GAAGGAGCAC 
CCCCTCTTCA AGAAGCTGAA CTTCAAGCGG CTGGGAGCTG GCATGCTGGA ACCACCCTTC 
AAGCCTGATC CCCAGGCCAT TTACTGCAAA GATGTTCTGG ACATTGAACA GTTCTCAACA 
GTTAAGGGTG TGGAGCTAGA GCCCACTGAC CAGGACTTCT ACCAGAAATT TGCCACAGGC 
AGTGTGCCCA TCCCTTGGCA GAACGAGATG GTGGAGACCG AGTGCTTCCA GGAGCTGAAC 
GTCTTCGGGC TGGATGGCTC AGTTCCCCCA GACCTAGACT GGAAGGGCCA GCCACCAGCA 
CCCCCCAAAA AGGGACTGCT GCAGAGACTC TTCAGTCGCC AGAGGACTGC TGTGGAAACT 
GCAGCGACAG TGAGGAAGAG CTTCCCGCCC GCCTCGAGCT CCCCACCCGC CTCTAGCCCC 
CAGCCTGGGG CCCCCCCGGG CGGTTGGCGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK6 ( XM_024994254.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850022.1 Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant X3, mRNA.

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CloneID	OBa236045
Clone ID Related Accession (Same CDS sequence)	XM_024994253.1 , XM_024994254.1
Accession Version	XM_024994254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	G protein-coupled receptor kinase 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATT TTCTGAGCCA CACGGGTCCT GACCTCATCC CCGAGGTCCC CCGGCAACTG 
GTGACGAACT GTGCCCAGCG GCTGGAGGAA GGACCCTGCA AAGACCTCTT CCAGGAGCTT 
ACTCGGTTGA CCCATGAGTA CCTGAGCATG GCCCCTTTTG CCGACTACCT CGACAGCATC 
TACTTCAACC GTTTTCTGCA GTGGAAGTGG CTGGAAAGGC AGCCAGTGAC CAAAAACACC 
TTCAGGCAGT ACCGAGTCCT GGGCAAAGGC GGCTTTGGGG AGGTATGCGC CTGCCAGGTG 
CGGGCCACAG GCAAGATGTA CGCCTGCAAG AAGCTGGAGA AGAAGCGGAT CAAGAAGCGG 
AAAGGGGAAG CCATGGCACT CAACGAGAAG CAGATTCTGG AGAAAGTGAA CAGTAGGTTT 
GTAGTGAGCT TGGCCTACGC CTATGAGACC AAGGATGCAC TGTGCCTGGT GCTGACGCTG 
ATGAACGGAG GCGACCTCAA GTTCCACATC TACCACATGG GCCAGGCTGG CTTCCCTGAG 
GCTCGGGCTG TCTTCTACGC AGCAGAGATC TGCTGTGGCC TGGAGGACCT GCACCGGGAG 
CGTATCGTGT ACAGGGACCT AAAGCCAGAG AACATCCTAC TAGATGACCA TGGTCACATC 
CGCATCTCTG ACCTGGGGCT GGCTGTGCAC GTACCCGAAG GCCAGACGAT CAAAGGCCGT 
GTGGGCACCG TGGGCTACAT GGCCCCAGAG GTGGTAAAAA ACGAACGGTA CACCTTTAGC 
CCGGACTGGT GGGCACTCGG CTGCCTCCTG TATGAGATGA TCGCAGGCCA GTCACCCTTC 
CAGCAGAGGA AAAAGAAGAT CAAGCGGGAG GAAGTGGAGC GGCTGGTGAA GGAGGTGCCT 
GAGGAGTACT CCGAGCACTT CTCCCCACAG GCCCGCTCAC TCTGCACCCA GCTCCTCTGC 
AAGGACCCCT CCGAACGCCT GGGGTGTGGT GGGGGCGGCG CGCAAGAAGT GAAGGAGCAC 
CCCCTCTTCA AGAAGCTGAA CTTCAAGCGG CTGGGAGCTG GCATGCTGGA ACCACCCTTC 
AAGCCTGATC CCCAGGCCAT TTACTGCAAA GATGTTCTGG ACATTGAACA GTTCTCAACA 
GTTAAGGGTG TGGAGCTAGA GCCCACTGAC CAGGACTTCT ACCAGAAATT TGCCACAGGC 
AGTGTGCCCA TCCCTTGGCA GAACGAGATG GTGGAGACCG AGTGCTTCCA GGAGCTGAAC 
GTCTTCGGGC TGGATGGCTC AGTTCCCCCA GACCTAGACT GGAAGGGCCA GCCACCAGCA 
CCCCCCAAAA AGGGACTGCT GCAGAGACTC TTCAGTCGCC AGAGGACTGC TGTGGAAACT 
GCAGCGACAG TGAGGAAGAG CTTCCCGCCC GCCTCGAGCT CCCCACCCGC CTCTAGCCCC 
CAGCCTGGGG CCCCCCCGGG CGGTTGGCGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850022.1
CDS	323..1795
Translation	MQNFLSHTGPDLIPEVPRQLVTNCAQRLEEGPCKDLFQELTRLTHEYLSMAPFADYLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYHMGQAGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVGYMAPEVVKNERYTFSPDWWALGCLLYEMIAGQSPFQQRKKKIKREEVERLVKEVPEEYSEHFSPQARSLCTQLLCKDPSERLGCGGGGAQEVKEHPLFKKLNFKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVELEPTDQDFYQKFATGSVPIPWQNEMVETECFQELNVFGLDGSVPPDLDWKGQPPAPPKKGLLQRLFSRQRTAVETAATVRKSFPPASSSPPASSPQPGAPPGGWR

Target ORF information:

RefSeq Version	XM_024994254.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994254.1

ORF Insert Sequence:

ATGCAGAATT TTCTGAGCCA CACGGGTCCT GACCTCATCC CCGAGGTCCC CCGGCAACTG 
GTGACGAACT GTGCCCAGCG GCTGGAGGAA GGACCCTGCA AAGACCTCTT CCAGGAGCTT 
ACTCGGTTGA CCCATGAGTA CCTGAGCATG GCCCCTTTTG CCGACTACCT CGACAGCATC 
TACTTCAACC GTTTTCTGCA GTGGAAGTGG CTGGAAAGGC AGCCAGTGAC CAAAAACACC 
TTCAGGCAGT ACCGAGTCCT GGGCAAAGGC GGCTTTGGGG AGGTATGCGC CTGCCAGGTG 
CGGGCCACAG GCAAGATGTA CGCCTGCAAG AAGCTGGAGA AGAAGCGGAT CAAGAAGCGG 
AAAGGGGAAG CCATGGCACT CAACGAGAAG CAGATTCTGG AGAAAGTGAA CAGTAGGTTT 
GTAGTGAGCT TGGCCTACGC CTATGAGACC AAGGATGCAC TGTGCCTGGT GCTGACGCTG 
ATGAACGGAG GCGACCTCAA GTTCCACATC TACCACATGG GCCAGGCTGG CTTCCCTGAG 
GCTCGGGCTG TCTTCTACGC AGCAGAGATC TGCTGTGGCC TGGAGGACCT GCACCGGGAG 
CGTATCGTGT ACAGGGACCT AAAGCCAGAG AACATCCTAC TAGATGACCA TGGTCACATC 
CGCATCTCTG ACCTGGGGCT GGCTGTGCAC GTACCCGAAG GCCAGACGAT CAAAGGCCGT 
GTGGGCACCG TGGGCTACAT GGCCCCAGAG GTGGTAAAAA ACGAACGGTA CACCTTTAGC 
CCGGACTGGT GGGCACTCGG CTGCCTCCTG TATGAGATGA TCGCAGGCCA GTCACCCTTC 
CAGCAGAGGA AAAAGAAGAT CAAGCGGGAG GAAGTGGAGC GGCTGGTGAA GGAGGTGCCT 
GAGGAGTACT CCGAGCACTT CTCCCCACAG GCCCGCTCAC TCTGCACCCA GCTCCTCTGC 
AAGGACCCCT CCGAACGCCT GGGGTGTGGT GGGGGCGGCG CGCAAGAAGT GAAGGAGCAC 
CCCCTCTTCA AGAAGCTGAA CTTCAAGCGG CTGGGAGCTG GCATGCTGGA ACCACCCTTC 
AAGCCTGATC CCCAGGCCAT TTACTGCAAA GATGTTCTGG ACATTGAACA GTTCTCAACA 
GTTAAGGGTG TGGAGCTAGA GCCCACTGAC CAGGACTTCT ACCAGAAATT TGCCACAGGC 
AGTGTGCCCA TCCCTTGGCA GAACGAGATG GTGGAGACCG AGTGCTTCCA GGAGCTGAAC 
GTCTTCGGGC TGGATGGCTC AGTTCCCCCA GACCTAGACT GGAAGGGCCA GCCACCAGCA 
CCCCCCAAAA AGGGACTGCT GCAGAGACTC TTCAGTCGCC AGAGGACTGC TGTGGAAACT 
GCAGCGACAG TGAGGAAGAG CTTCCCGCCC GCCTCGAGCT CCCCACCCGC CTCTAGCCCC 
CAGCCTGGGG CCCCCCCGGG CGGTTGGCGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK6 ( XM_024994251.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850019.1 Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant X1, mRNA.

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CloneID	OBa236044
Clone ID Related Accession (Same CDS sequence)	XM_024994251.1
Accession Version	XM_024994251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	G protein-coupled receptor kinase 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCG AGAACATCGT AGCGAACACG GTGCTACTCA AGGCCCGGGA AGGTGGTGGC 
GGGAATCGTA AAGGCAAAAG CAAGAAATGG CGGCAGATGC TACAGTTCCC CCATATCAGC 
CAGTGTGAAG AGCTACGGCT CAGCCTCGAG CGTGACTACC ACAGCCTGTG TGAGCGGCAG 
CCCATTGGGC GCCTGCTGTT CCGAGAGTTC TGCGCCACGA GGCCTGAGCT GACTCGCTGC 
ATTGCCTTCC TGGACGGGGT GGCCGAGTAT GAAGTGACCC CTGATGAGAA GCGGAAGGCA 
TGTGGGCTGC GGCTCATGCA GAATTTTCTG AGCCACACGG GTCCTGACCT CATCCCCGAG 
GTCCCCCGGC AACTGGTGAC GAACTGTGCC CAGCGGCTGG AGGAAGGACC CTGCAAAGAC 
CTCTTCCAGG AGCTTACTCG GTTGACCCAT GAGTACCTGA GCATGGCCCC TTTTGCCGAC 
TACCTCGACA GCATCTACTT CAACCGTTTT CTGCAGTGGA AGTGGCTGGA AAGGCAGCCA 
GTGACCAAAA ACACCTTCAG GCAGTACCGA GTCCTGGGCA AAGGCGGCTT TGGGGAGGTA 
TGCGCCTGCC AGGTGCGGGC CACAGGCAAG ATGTACGCCT GCAAGAAGCT GGAGAAGAAG 
CGGATCAAGA AGCGGAAAGG GGAAGCCATG GCACTCAACG AGAAGCAGAT TCTGGAGAAA 
GTGAACAGTA GGTTTGTAGT GAGCTTGGCC TACGCCTATG AGACCAAGGA TGCACTGTGC 
CTGGTGCTGA CGCTGATGAA CGGAGGCGAC CTCAAGTTCC ACATCTACCA CATGGGCCAG 
GCTGGCTTCC CTGAGGCTCG GGCTGTCTTC TACGCAGCAG AGATCTGCTG TGGCCTGGAG 
GACCTGCACC GGGAGCGTAT CGTGTACAGG GACCTAAAGC CAGAGAACAT CCTACTAGAT 
GACCATGGTC ACATCCGCAT CTCTGACCTG GGGCTGGCTG TGCACGTACC CGAAGGCCAG 
ACGATCAAAG GCCGTGTGGG CACCGTGGGC TACATGGCCC CAGAGGTGGT AAAAAACGAA 
CGGTACACCT TTAGCCCGGA CTGGTGGGCA CTCGGCTGCC TCCTGTATGA GATGATCGCA 
GGCCAGTCAC CCTTCCAGCA GAGGAAAAAG AAGATCAAGC GGGAGGAAGT GGAGCGGCTG 
GTGAAGGAGG TGCCTGAGGA GTACTCCGAG CACTTCTCCC CACAGGCCCG CTCACTCTGC 
ACCCAGCTCC TCTGCAAGGA CCCCTCCGAA CGCCTGGGGT GTGGTGGGGG CGGCGCGCAA 
GAAGTGAAGG AGCACCCCCT CTTCAAGAAG CTGAACTTCA AGCGGCTGGG AGCTGGCATG 
CTGGAACCAC CCTTCAAGCC TGATCCCCAG GCCATTTACT GCAAAGATGT TCTGGACATT 
GAACAGTTCT CAACAGTTAA GGGTGTGGAG CTAGAGCCCA CTGACCAGGA CTTCTACCAG 
AAATTTGCCA CAGGCAGTGT GCCCATCCCT TGGCAGAACG AGATGGTGGA GACCGAGTGC 
TTCCAGGAGC TGAACGTCTT CGGGCTGGAT GGCTCAGTTC CCCCAGACCT AGACTGGAAG 
GGCCAGCCAC CAGCACCCCC CAAAAAGGGA CTGCTGCAGA GACTCTTCAG TCGCCAGAGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850019.1
CDS	159..1841
Translation	MELENIVANTVLLKAREGGGGNRKGKSKKWRQMLQFPHISQCEELRLSLERDYHSLCERQPIGRLLFREFCATRPELTRCIAFLDGVAEYEVTPDEKRKACGLRLMQNFLSHTGPDLIPEVPRQLVTNCAQRLEEGPCKDLFQELTRLTHEYLSMAPFADYLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYHMGQAGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVGYMAPEVVKNERYTFSPDWWALGCLLYEMIAGQSPFQQRKKKIKREEVERLVKEVPEEYSEHFSPQARSLCTQLLCKDPSERLGCGGGGAQEVKEHPLFKKLNFKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVELEPTDQDFYQKFATGSVPIPWQNEMVETECFQELNVFGLDGSVPPDLDWKGQPPAPPKKGLLQRLFSRQR

Target ORF information:

RefSeq Version	XM_024994251.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024994251.1

ORF Insert Sequence:

ATGGAGCTCG AGAACATCGT AGCGAACACG GTGCTACTCA AGGCCCGGGA AGGTGGTGGC 
GGGAATCGTA AAGGCAAAAG CAAGAAATGG CGGCAGATGC TACAGTTCCC CCATATCAGC 
CAGTGTGAAG AGCTACGGCT CAGCCTCGAG CGTGACTACC ACAGCCTGTG TGAGCGGCAG 
CCCATTGGGC GCCTGCTGTT CCGAGAGTTC TGCGCCACGA GGCCTGAGCT GACTCGCTGC 
ATTGCCTTCC TGGACGGGGT GGCCGAGTAT GAAGTGACCC CTGATGAGAA GCGGAAGGCA 
TGTGGGCTGC GGCTCATGCA GAATTTTCTG AGCCACACGG GTCCTGACCT CATCCCCGAG 
GTCCCCCGGC AACTGGTGAC GAACTGTGCC CAGCGGCTGG AGGAAGGACC CTGCAAAGAC 
CTCTTCCAGG AGCTTACTCG GTTGACCCAT GAGTACCTGA GCATGGCCCC TTTTGCCGAC 
TACCTCGACA GCATCTACTT CAACCGTTTT CTGCAGTGGA AGTGGCTGGA AAGGCAGCCA 
GTGACCAAAA ACACCTTCAG GCAGTACCGA GTCCTGGGCA AAGGCGGCTT TGGGGAGGTA 
TGCGCCTGCC AGGTGCGGGC CACAGGCAAG ATGTACGCCT GCAAGAAGCT GGAGAAGAAG 
CGGATCAAGA AGCGGAAAGG GGAAGCCATG GCACTCAACG AGAAGCAGAT TCTGGAGAAA 
GTGAACAGTA GGTTTGTAGT GAGCTTGGCC TACGCCTATG AGACCAAGGA TGCACTGTGC 
CTGGTGCTGA CGCTGATGAA CGGAGGCGAC CTCAAGTTCC ACATCTACCA CATGGGCCAG 
GCTGGCTTCC CTGAGGCTCG GGCTGTCTTC TACGCAGCAG AGATCTGCTG TGGCCTGGAG 
GACCTGCACC GGGAGCGTAT CGTGTACAGG GACCTAAAGC CAGAGAACAT CCTACTAGAT 
GACCATGGTC ACATCCGCAT CTCTGACCTG GGGCTGGCTG TGCACGTACC CGAAGGCCAG 
ACGATCAAAG GCCGTGTGGG CACCGTGGGC TACATGGCCC CAGAGGTGGT AAAAAACGAA 
CGGTACACCT TTAGCCCGGA CTGGTGGGCA CTCGGCTGCC TCCTGTATGA GATGATCGCA 
GGCCAGTCAC CCTTCCAGCA GAGGAAAAAG AAGATCAAGC GGGAGGAAGT GGAGCGGCTG 
GTGAAGGAGG TGCCTGAGGA GTACTCCGAG CACTTCTCCC CACAGGCCCG CTCACTCTGC 
ACCCAGCTCC TCTGCAAGGA CCCCTCCGAA CGCCTGGGGT GTGGTGGGGG CGGCGCGCAA 
GAAGTGAAGG AGCACCCCCT CTTCAAGAAG CTGAACTTCA AGCGGCTGGG AGCTGGCATG 
CTGGAACCAC CCTTCAAGCC TGATCCCCAG GCCATTTACT GCAAAGATGT TCTGGACATT 
GAACAGTTCT CAACAGTTAA GGGTGTGGAG CTAGAGCCCA CTGACCAGGA CTTCTACCAG 
AAATTTGCCA CAGGCAGTGT GCCCATCCCT TGGCAGAACG AGATGGTGGA GACCGAGTGC 
TTCCAGGAGC TGAACGTCTT CGGGCTGGAT GGCTCAGTTC CCCCAGACCT AGACTGGAAG 
GGCCAGCCAC CAGCACCCCC CAAAAAGGGA CTGCTGCAGA GACTCTTCAG TCGCCAGAGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK6 ( NM_001191530.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178459.1 Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant 1, mRNA.

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CloneID	OBa37060
Clone ID Related Accession (Same CDS sequence)	NM_001191530.1
Accession Version	NM_001191530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Bos taurus(cattle)
Product	G protein-coupled receptor kinase 6 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000007.1, DY071905.1, BF605412.1, CO873051.1, DY121035.1, DY094222.1, EV633678.1, DY158204.1 and CO876214.1. On Apr 20, 2011 this sequence version replaced XM_002704831.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCTCG AGAACATCGT AGCGAACACG GTGCTACTCA AGGCCCGGGA AGGTGGTGGC 
GGGAATCGTA AAGGCAAAAG CAAGAAATGG CGGCAGATGC TACAGTTCCC CCATATCAGC 
CAGTGTGAAG AGCTACGGCT CAGCCTCGAG CGTGACTACC ACAGCCTGTG TGAGCGGCAG 
CCCATTGGGC GCCTGCTGTT CCGAGAGTTC TGCGCCACGA GGCCTGAGCT GACTCGCTGC 
ATTGCCTTCC TGGACGGGGT GGCCGAGTAT GAAGTGACCC CTGATGAGAA GCGGAAGGCA 
TGTGGGCTGC GGCTCATGCA GAATTTTCTG AGCCACACGG GTCCTGACCT CATCCCCGAG 
GTCCCCCGGC AACTGGTGAC GAACTGTGCC CAGCGGCTGG AGGAAGGACC CTGCAAAGAC 
CTCTTCCAGG AGCTTACTCG GTTGACCCAT GAGTACCTGA GCATGGCCCC TTTTGCCGAC 
TACCTCGACA GCATCTACTT CAACCGTTTT CTGCAGTGGA AGTGGCTGGA AAGGCAGCCA 
GTGACCAAAA ACACCTTCAG GCAGTACCGA GTCCTGGGCA AAGGCGGCTT TGGGGAGGTA 
TGCGCCTGCC AGGTGCGGGC CACAGGCAAG ATGTACGCCT GCAAGAAGCT GGAGAAGAAG 
CGGATCAAGA AGCGGAAAGG GGAAGCCATG GCACTCAACG AGAAGCAGAT TCTGGAGAAA 
GTGAACAGTA GGTTTGTAGT GAGCTTGGCC TACGCCTATG AGACCAAGGA TGCACTGTGC 
CTGGTGCTGA CGCTGATGAA CGGAGGCGAC CTCAAGTTCC ACATCTACCA CATGGGCCAG 
GCTGGCTTCC CTGAGGCTCG GGCTGTCTTC TACGCAGCAG AGATCTGCTG TGGCCTGGAG 
GACCTGCACC GGGAGCGTAT CGTGTACAGG GACCTAAAGC CAGAGAACAT CCTACTAGAT 
GACCATGGTC ACATCCGCAT CTCTGACCTG GGGCTGGCTG TGCACGTACC CGAAGGCCAG 
ACGATCAAAG GCCGTGTGGG CACCGTGGGC TACATGGCCC CAGAGGTGGT AAAAAACGAA 
CGGTACACCT TTAGCCCGGA CTGGTGGGCA CTCGGCTGCC TCCTGTATGA GATGATCGCA 
GGCCAGTCAC CCTTCCAGCA GAGGAAAAAG AAGATCAAGC GGGAGGAAGT GGAGCGGCTG 
GTGAAGGAGG TGCCTGAGGA GTACTCCGAG CACTTCTCCC CACAGGCCCG CTCACTCTGC 
ACCCAGCTCC TCTGCAAGGA CCCCTCCGAA CGCCTGGGGT GTGGTGGGGG CGGCGCGCAA 
GAAGTGAAGG AGCACCCCCT CTTCAAGAAG CTGAACTTCA AGCGGCTGGG AGCTGGCATG 
CTGGAACCAC CCTTCAAGCC TGATCCCCAG GCCATTTACT GCAAAGATGT TCTGGACATT 
GAACAGTTCT CAACAGTTAA GGGTGTGGAG CTAGAGCCCA CTGACCAGGA CTTCTACCAG 
AAATTTGCCA CAGGCAGTGT GCCCATCCCT TGGCAGAACG AGATGGTGGA GACCGAGTGC 
TTCCAGGAGC TGAACGTCTT CGGGCTGGAT GGCTCAGTTC CCCCAGACCT AGACTGGAAG 
GGCCAGCCAC CAGCACCCCC CAAAAAGGGA CTGCTGCAGA GACTCTTCAG TCGCCAGAGG 
ACTGCTGTGG AAACTGCAGC GACAGTGAGG AAGAGCTTCC CGCCCGCCTC GAGCTCCCCA 
CCCGCCTCTA GCCCCCAGCC TGGGGCCCCC CCGGGCGGTT GGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178459.1
CDS	101..1888
Translation	MELENIVANTVLLKAREGGGGNRKGKSKKWRQMLQFPHISQCEELRLSLERDYHSLCERQPIGRLLFREFCATRPELTRCIAFLDGVAEYEVTPDEKRKACGLRLMQNFLSHTGPDLIPEVPRQLVTNCAQRLEEGPCKDLFQELTRLTHEYLSMAPFADYLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYHMGQAGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVGYMAPEVVKNERYTFSPDWWALGCLLYEMIAGQSPFQQRKKKIKREEVERLVKEVPEEYSEHFSPQARSLCTQLLCKDPSERLGCGGGGAQEVKEHPLFKKLNFKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVELEPTDQDFYQKFATGSVPIPWQNEMVETECFQELNVFGLDGSVPPDLDWKGQPPAPPKKGLLQRLFSRQRTAVETAATVRKSFPPASSSPPASSPQPGAPPGGWR

Target ORF information:

RefSeq Version	NM_001191530.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191530.1

ORF Insert Sequence:

ATGGAGCTCG AGAACATCGT AGCGAACACG GTGCTACTCA AGGCCCGGGA AGGTGGTGGC 
GGGAATCGTA AAGGCAAAAG CAAGAAATGG CGGCAGATGC TACAGTTCCC CCATATCAGC 
CAGTGTGAAG AGCTACGGCT CAGCCTCGAG CGTGACTACC ACAGCCTGTG TGAGCGGCAG 
CCCATTGGGC GCCTGCTGTT CCGAGAGTTC TGCGCCACGA GGCCTGAGCT GACTCGCTGC 
ATTGCCTTCC TGGACGGGGT GGCCGAGTAT GAAGTGACCC CTGATGAGAA GCGGAAGGCA 
TGTGGGCTGC GGCTCATGCA GAATTTTCTG AGCCACACGG GTCCTGACCT CATCCCCGAG 
GTCCCCCGGC AACTGGTGAC GAACTGTGCC CAGCGGCTGG AGGAAGGACC CTGCAAAGAC 
CTCTTCCAGG AGCTTACTCG GTTGACCCAT GAGTACCTGA GCATGGCCCC TTTTGCCGAC 
TACCTCGACA GCATCTACTT CAACCGTTTT CTGCAGTGGA AGTGGCTGGA AAGGCAGCCA 
GTGACCAAAA ACACCTTCAG GCAGTACCGA GTCCTGGGCA AAGGCGGCTT TGGGGAGGTA 
TGCGCCTGCC AGGTGCGGGC CACAGGCAAG ATGTACGCCT GCAAGAAGCT GGAGAAGAAG 
CGGATCAAGA AGCGGAAAGG GGAAGCCATG GCACTCAACG AGAAGCAGAT TCTGGAGAAA 
GTGAACAGTA GGTTTGTAGT GAGCTTGGCC TACGCCTATG AGACCAAGGA TGCACTGTGC 
CTGGTGCTGA CGCTGATGAA CGGAGGCGAC CTCAAGTTCC ACATCTACCA CATGGGCCAG 
GCTGGCTTCC CTGAGGCTCG GGCTGTCTTC TACGCAGCAG AGATCTGCTG TGGCCTGGAG 
GACCTGCACC GGGAGCGTAT CGTGTACAGG GACCTAAAGC CAGAGAACAT CCTACTAGAT 
GACCATGGTC ACATCCGCAT CTCTGACCTG GGGCTGGCTG TGCACGTACC CGAAGGCCAG 
ACGATCAAAG GCCGTGTGGG CACCGTGGGC TACATGGCCC CAGAGGTGGT AAAAAACGAA 
CGGTACACCT TTAGCCCGGA CTGGTGGGCA CTCGGCTGCC TCCTGTATGA GATGATCGCA 
GGCCAGTCAC CCTTCCAGCA GAGGAAAAAG AAGATCAAGC GGGAGGAAGT GGAGCGGCTG 
GTGAAGGAGG TGCCTGAGGA GTACTCCGAG CACTTCTCCC CACAGGCCCG CTCACTCTGC 
ACCCAGCTCC TCTGCAAGGA CCCCTCCGAA CGCCTGGGGT GTGGTGGGGG CGGCGCGCAA 
GAAGTGAAGG AGCACCCCCT CTTCAAGAAG CTGAACTTCA AGCGGCTGGG AGCTGGCATG 
CTGGAACCAC CCTTCAAGCC TGATCCCCAG GCCATTTACT GCAAAGATGT TCTGGACATT 
GAACAGTTCT CAACAGTTAA GGGTGTGGAG CTAGAGCCCA CTGACCAGGA CTTCTACCAG 
AAATTTGCCA CAGGCAGTGT GCCCATCCCT TGGCAGAACG AGATGGTGGA GACCGAGTGC 
TTCCAGGAGC TGAACGTCTT CGGGCTGGAT GGCTCAGTTC CCCCAGACCT AGACTGGAAG 
GGCCAGCCAC CAGCACCCCC CAAAAAGGGA CTGCTGCAGA GACTCTTCAG TCGCCAGAGG 
ACTGCTGTGG AAACTGCAGC GACAGTGAGG AAGAGCTTCC CGCCCGCCTC GAGCTCCCCA 
CCCGCCTCTA GCCCCCAGCC TGGGGCCCCC CCGGGCGGTT GGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK6 ( XM_015472157.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327643.1 Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant X1, mRNA.

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CloneID	OBa91111
Clone ID Related Accession (Same CDS sequence)	XM_015472157.1
Accession Version	XM_015472157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G protein-coupled receptor kinase 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104024.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCG AGAACATCGT AGCGAACACG GTGCTACTCA AGGCCCGGGA AGGTGGTGGC 
GGGAATCGTA AAGGCAAAAG CAAGAAATGG CGGCAGATGC TACAGTTCCC CCATATCAGC 
CAGTGTGAAG AGCTACGGCT CAGCCTCGAG CGTGACTACC ACAGCCTGTG TGAGCGGCAG 
CCCATTGGGC GCCTGCTGTT CCGAGAGTTC TGCGCCACGA GGCCTGAGCT GACTCGCTGC 
ATTGCCTTCC TGGACGGGGT GGCCGAGTAT GAAGTGACCC CTGATGAGAA GCGGAAGGCA 
TGTGGGCTGC GGCTCATGCA GAATTTTCTG AGCCACACGG GTCCTGACCT CATCCCCGAG 
GTCCCCCGGC AACTGGTGAC GAACTGTGCC CAGCGGCTGG AGGAAGGACC CTGCAAAGAC 
CTCTTCCAGG AGCTTACTCG GTTGACCCAT GAGTACCTGA GCATGGCCCC TTTTGCCGAC 
TACCTCGACA GCATCTACTT CAACCGTTTT CTGCAGTGGA AGTGGCTGGA AAGGCAGCCA 
GTGACCAAAA ACACCTTCAG GCAGTACCGA GTCCTGGGCA AAGGCGGCTT TGGGGAGGTG 
AGTATGCGCT GCCAGGTGCG GGCCACAGGC AAGATGTACG CCTGCAAGAA GCTGGAGAAG 
AAGCGGATCA AGAAGCGGAA AGGGGAAGCC ATGGCACTCA ACGAGAAGCA GATTCTGGAG 
AAAGTGAACA GTAGGTTTGT AGTGAGCTTG GCCTACGCCT ATGAGACCAA GGATGCACTG 
TGCCTGGTGC TGACGCTGAT GAACGGAGGC GACCTCAAGT TCCACATCTA CCACATGGGC 
CAGGCTGGCT TCCCTGAGGC TCGGGCTGTC TTCTACGCAG CAGAGATCTG CTGTGGCCTG 
GAGGACCTGC ACCGGGAGCG TATCGTGTAC AGGGACCTAA AGCCAGAGAA CATCCTACTA 
GATGACCATG GTCACATCCG CATCTCTGAC CTGGGGCTGG CTGTGCACGT ACCCGAAGGC 
CAGACGATCA AAGGCCGTGT GGGCACCGTG GGCTACATGG CCCCAGAGGT GGTAAAAAAC 
GAACGGTACA CCTTTAGCCC GGACTGGTGG GCACTCGGCT GCCTCCTGTA TGAGATGATC 
GCAGGCCAGT CACCCTTCCA GCAGAGGAAA AAGAAGATCA AGCGGGAGGA AGTGGAGCGG 
CTGGTGAAGG AGGTGCCTGA GGAGTACTCC GAGCACTTCT CCCCACAGGC CCGCTCACTC 
TGCACCCAGC TCCTCTGCAA GGACCCCTCC GAACGCCTGG GGTGTGGTGG GGGCGGCGCG 
CAAGAAGTGA AGGAGCACCC CCTCTTCAAG AAGCTGAACT TCAAGCGGCT GGGAGCTGGC 
ATGCTGGAAC CACCCTTCAA GCCTGATCCC CAGGCCATTT ACTGCAAAGA TGTTCTGGAC 
ATTGAACAGT TCTCAACAGT TAAGGGTGTG GAGCTAGAGC CCACTGACCA GGACTTCTAC 
CAGAAATTTG CCACAGGCAG TGTGCCCATC CCTTGGCAGA ACGAGATGGT GGAGACCGAG 
TGCTTCCAGG AGCTGAACGT CTTCGGGCTG GATGGCTCAG TTCCCCCAGA CCTAGACTGG 
AAGGGCCAGC CACCAGCACC CCCCAAAAAG GGACTGCTGC AGAGACTCTT CAGTCGCCAG 
CGGACTGCTG TGGAAACTGC AGCGACAGTG AGGAAGAGCT TCCCGCCCGC CTCGAGCTCC 
CCACCCGCCT CTAGCCCCCA GCCTGGGGCC CCCCCGGGCG GTTGGCGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327643.1
CDS	143..1933
Translation	MELENIVANTVLLKAREGGGGNRKGKSKKWRQMLQFPHISQCEELRLSLERDYHSLCERQPIGRLLFREFCATRPELTRCIAFLDGVAEYEVTPDEKRKACGLRLMQNFLSHTGPDLIPEVPRQLVTNCAQRLEEGPCKDLFQELTRLTHEYLSMAPFADYLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVSMRCQVRATGKMYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYHMGQAGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVGYMAPEVVKNERYTFSPDWWALGCLLYEMIAGQSPFQQRKKKIKREEVERLVKEVPEEYSEHFSPQARSLCTQLLCKDPSERLGCGGGGAQEVKEHPLFKKLNFKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVELEPTDQDFYQKFATGSVPIPWQNEMVETECFQELNVFGLDGSVPPDLDWKGQPPAPPKKGLLQRLFSRQRTAVETAATVRKSFPPASSSPPASSPQPGAPPGGWR

Target ORF information:

RefSeq Version	XM_015472157.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 6 (GRK6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472157.1

ORF Insert Sequence:

ATGGAGCTCG AGAACATCGT AGCGAACACG GTGCTACTCA AGGCCCGGGA AGGTGGTGGC 
GGGAATCGTA AAGGCAAAAG CAAGAAATGG CGGCAGATGC TACAGTTCCC CCATATCAGC 
CAGTGTGAAG AGCTACGGCT CAGCCTCGAG CGTGACTACC ACAGCCTGTG TGAGCGGCAG 
CCCATTGGGC GCCTGCTGTT CCGAGAGTTC TGCGCCACGA GGCCTGAGCT GACTCGCTGC 
ATTGCCTTCC TGGACGGGGT GGCCGAGTAT GAAGTGACCC CTGATGAGAA GCGGAAGGCA 
TGTGGGCTGC GGCTCATGCA GAATTTTCTG AGCCACACGG GTCCTGACCT CATCCCCGAG 
GTCCCCCGGC AACTGGTGAC GAACTGTGCC CAGCGGCTGG AGGAAGGACC CTGCAAAGAC 
CTCTTCCAGG AGCTTACTCG GTTGACCCAT GAGTACCTGA GCATGGCCCC TTTTGCCGAC 
TACCTCGACA GCATCTACTT CAACCGTTTT CTGCAGTGGA AGTGGCTGGA AAGGCAGCCA 
GTGACCAAAA ACACCTTCAG GCAGTACCGA GTCCTGGGCA AAGGCGGCTT TGGGGAGGTG 
AGTATGCGCT GCCAGGTGCG GGCCACAGGC AAGATGTACG CCTGCAAGAA GCTGGAGAAG 
AAGCGGATCA AGAAGCGGAA AGGGGAAGCC ATGGCACTCA ACGAGAAGCA GATTCTGGAG 
AAAGTGAACA GTAGGTTTGT AGTGAGCTTG GCCTACGCCT ATGAGACCAA GGATGCACTG 
TGCCTGGTGC TGACGCTGAT GAACGGAGGC GACCTCAAGT TCCACATCTA CCACATGGGC 
CAGGCTGGCT TCCCTGAGGC TCGGGCTGTC TTCTACGCAG CAGAGATCTG CTGTGGCCTG 
GAGGACCTGC ACCGGGAGCG TATCGTGTAC AGGGACCTAA AGCCAGAGAA CATCCTACTA 
GATGACCATG GTCACATCCG CATCTCTGAC CTGGGGCTGG CTGTGCACGT ACCCGAAGGC 
CAGACGATCA AAGGCCGTGT GGGCACCGTG GGCTACATGG CCCCAGAGGT GGTAAAAAAC 
GAACGGTACA CCTTTAGCCC GGACTGGTGG GCACTCGGCT GCCTCCTGTA TGAGATGATC 
GCAGGCCAGT CACCCTTCCA GCAGAGGAAA AAGAAGATCA AGCGGGAGGA AGTGGAGCGG 
CTGGTGAAGG AGGTGCCTGA GGAGTACTCC GAGCACTTCT CCCCACAGGC CCGCTCACTC 
TGCACCCAGC TCCTCTGCAA GGACCCCTCC GAACGCCTGG GGTGTGGTGG GGGCGGCGCG 
CAAGAAGTGA AGGAGCACCC CCTCTTCAAG AAGCTGAACT TCAAGCGGCT GGGAGCTGGC 
ATGCTGGAAC CACCCTTCAA GCCTGATCCC CAGGCCATTT ACTGCAAAGA TGTTCTGGAC 
ATTGAACAGT TCTCAACAGT TAAGGGTGTG GAGCTAGAGC CCACTGACCA GGACTTCTAC 
CAGAAATTTG CCACAGGCAG TGTGCCCATC CCTTGGCAGA ACGAGATGGT GGAGACCGAG 
TGCTTCCAGG AGCTGAACGT CTTCGGGCTG GATGGCTCAG TTCCCCCAGA CCTAGACTGG 
AAGGGCCAGC CACCAGCACC CCCCAAAAAG GGACTGCTGC AGAGACTCTT CAGTCGCCAG 
CGGACTGCTG TGGAAACTGC AGCGACAGTG AGGAAGAGCT TCCCGCCCGC CTCGAGCTCC 
CCACCCGCCT CTAGCCCCCA GCCTGGGGCC CCCCCGGGCG GTTGGCGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
BMC genomics7283(2006 Nov)
Jann OC,Aerts J,Jones M,Hastings N,Law A,McKay S,Marques E,Prasad A,Yu J,Moore SS,Floriot S,Mah? MF,Eggen A,Silveri L,Negrini R,Milanesi E,Ajmone-Marsan P,Valentini A,Marchitelli C,Savarese MC,Janitz M,Herwig R,Hennig S,Gorni C,Connor EE,Sonstegard TS,Smith T,Dr?gem?ller C,Williams JL

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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GRK6 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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