VPS13A cDNA ORF clone, Bos taurus(cattle)

The following VPS13A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS13A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa50054 XM_010807909.2
Latest version!
Bos taurus vacuolar protein sorting 13 homolog A (S. cerevisiae) (VPS13A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50054 XM_015464540.1
Latest version!
Bos taurus vacuolar protein sorting 13 homolog A (S. cerevisiae) (VPS13A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50055 XM_015464541.1
Latest version!
Bos taurus vacuolar protein sorting 13 homolog A (S. cerevisiae) (VPS13A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50055 XM_010807910.2
Latest version!
Bos taurus vacuolar protein sorting 13 homolog A (S. cerevisiae) (VPS13A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa237445 XM_024996188.1
Latest version!
Bos taurus vacuolar protein sorting 13 homolog A (VPS13A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa237445 XM_024996187.1
Latest version!
Bos taurus vacuolar protein sorting 13 homolog A (VPS13A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa50054
    Clone ID Related Accession (Same CDS sequence) XM_015464540.1 , XM_010807909.2
    Accession Version XM_015464540.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product vacuolar protein sorting-associated protein 13A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAAAAGA AAGCCAAAAA GGCCATTGTT GAGTCAGGTA CTGAAGAAGA AAATTACAGA 
    GTACCAGAAT ACAAAACTGT CATCACTTTC TATTCGAAAG ACCAGCTAAA CATTACATTA
    TCTAAATGTG GCCTTATAAT GTTAAATAAT TTAGCTGAGG CATTTACAGA AGCTGCCACT
    GGATCTTCAG CTACCTTCAT AAGGGATCTG GCACCTTTTA TGATTTTAAA TTCTCTTGGA
    CTTAGTATTT CTGTTTCACC AAGTGAATCT TTTAGTGTAC TCAACGTACC TATGGCAAAA
    TCATATGTAT TGAAAAATGA GGAGAGTTTA AGTATGGATT ATATGCGAAC TAAAGACAAT
    GATCATTTCA ATGCAATGAC CAGCCTAAGC AGCAAACACT TCTTCATTCT TCTTACCCCT
    GCTAACCACT CTACTGCTGA TAAAATCCCT TTAACAAAAG TGGGACGACG ACTGTACACT
    GTAAGACACA GAGAGTCTGG AGTTGAAAGA TCAATTGTTT GTCAGATTGA TGCAGTGGAA
    GGAAGTAAGA AGGTAACAGT TCGCTCTCCA GTGCAGATTA GAAATCATTT TTCAATACCA
    CTTTCTGTTT ATGGAGGAGA CACGTTATTG GGAACGGCCT CACCTGGAAG TGAATTTAAC
    ATACCATTAG CATCTTACCG ATCACTACTT TCTCTGAAGC CAGAAGATGC AGACTATCAG
    ATGTGTGAAG GAATTGACTT TGAAGAAATT ATAAAACAAG ATGGTACTTC TTTCAAGAAA
    AAATGTAGAC CTATAAACCC TTCCAGGAAG CCATTTATCA TTAATATAGT CCCAGAAAAA
    GATAATTTGA CGTATATGTC AGTGTATTCA GAAGACCGTT GGGACTTACC ATATATAATA
    CATTTGTGGC CACCTATTCT ACTCCGAAAT CTTCTTCCTT ACAAAATTGC TTATTACATA
    GAGGGGATTG AACATAGAGT TTTTACTCTC CATGAAGGCC GTTCAGCCCA GCTTTGTACT
    GTACAGTTGG ATAAAGCCAG GCTACATTTA AAATTACTTG ACTATCTTAA TCATGATTGG
    AAAAGTGAAT ATCATATAAA ACCTAATCAA CAAGATATTA GTTTCATCAG TTTTACTTGT
    GTTACAGAAA TAGAAAAGAC TGATTTAGAT ATTGCTGTCC ATGTGACTTA TAATACTGGC
    CAGACTATTA TGGCATTTCA TAGTCCTTAT TGGATGGTTA ATAAAACTGG TCGAATGTTG
    CAGTATAAAG CAGATGGAAT TCATCGAAAG CATCCACCTA ATTATAAAAA GCCAGTTCTC
    TTTTCATTTC AGCCAAAGCA CTTTTTCAAT AACAATAAGG TTCAGCTTAT GGTAACTGAT
    AGTGAATTGT CAGATCAATT TTCAATTGAT ACAGTTGGTA GTCATGGAGC GGTTAAATGT
    AAAGGCCCAA AAATGGAATA TCAAGTTGGT GTCACTATAG ACCTTAGCAG TTTTAACATT
    ACCAGAATTG TCACATTTAC CCCCTTTTTT TTGATTAAAA ACAAAAGCAA ATATCATATA
    TCAGTGGCTG AAGAGGGAAA TGATAAATGG CTCGCTCTTG ATTTGGAGCA GTGTGTCCCC
    TTTTGGCCTG AGGATGCTTC TAATAAACTT CTTATTCGAG TTGAAAGAAG TGAAAGCCCT
    CCCCATAAGA TACATTTTAA TAAGGAAGAA AGTTGTATTT TACTGCGTCT AGACAATGAG
    CTTGGAGGTA TTATAGCAGA AGTTAATTTG GCTGAGCATT CTACAGTTAT TACATTTTCA
    GATTATCGTG ATGGAGCCGC TACATTTCTG TTAATAAATC ACACCAAGAA TGACATTGTT
    CAGTATAAGC AAAGTTCCCT CAGTGAAATA GAAGATTCCC TCCCTCCTGG AAAAGCAGTA
    TTTTATACAT GGGCTGATCC AGTGGGCTCT AGAAAGCTGC AGTGGAAATG TGGAAAAAGC
    CACGGTGAAG TAACACAAAA GGATGATATG ATGACACCTA TAAATTTGGG GAAAACGACC
    ATTTATTTAG TTTCATTTTT TGAAGGCTTA CAACGCATAA TTTTATTCAC TGAAGATCCA
    AGAGTATTTA AAGTGACATA TGAAAGTGAG AAAGCAGAGT TAGCAGAGCA AGAAATTGTG
    TTGGCGTTAC AAGATGTTGG GATTTCTCTT GTCAACAATT ATACAAAGCA AGAAGTAGCC
    TATATTGGCA TTACAAGTTC TGATGTGGTT TGGGAGACAA AGCCCAGGAA GAAGGCAAGA
    TGGAAGCCAA TGAGTGTAAA GCACACTGAG AAGTTAGAGA AGGAATTTAA GGAATATGTT
    GAATCTTCAC CGTCAGAAGA TAAGGTTATT GAGTTGGACA CCAACATTCT GGTTCGCTTC
    ACACCTGGTG CTTCTAACAT GAAAATTCTG CAGCCACATG TAATAGCTAT AAGAAGAAAT
    TATCTTCCAG CATTAAAAGT AGAATATAGC ACATCTGCAC ATCAGTCATC GTTTAGAATT
    CAAATTTATA GAATACAGAT CCAAAACCAA ATACATGGTG CTATATTTCC ATTCGTGTTT
    TATCCCATTA AACCGCCCAA GTCCGTCACC TTGGATTCAG CACCGAAACC CTTTACTGAT
    GTCAGTATTG TCATGAGATC TGCAGGACAT TCGCAGATAT CACGTATTAA GTATTTCAAA
    GTGTTGATCC AAGAAATGGA TCTCAGGTTA GATCTTGGAT TTGTCTATGC TGTATCAGAA
    CTTATGACAC CAGCTGAAGT CACTGAAAAA ACAGAGGTTG AGCTTTTTCA TAAAGACATA
    GAAGCTTTCC AAGAAGAATA TAAAATAGTC TCATTAGTAG ATTCATCACA AGTCAGTCTC
    TATGAATACT TTCATATATC TCCTATCAAG TTGCATTTAA GTGTTTCACT GAGTTCAGGT
    GGTGAAGAAC CGAAAGATTC AGAACAGCAT GAAGGACTGA TGGCAATTCG TTCTTTACAT
    CTTTTGCTGA AGAGTATTGG TGCCACTCTC ACAGATGTAC AAGATGTAGT TTTTAAGCTA
    GCATTTTTTG AACTCAATTA TCAGTTCCAT ACAACAGCTG AACTACAGTC TGAAGTAATA
    AGACACTATT CAAAGCAGGC CATTAAACAG ATGTACGTAC TCATTCTTGG ACTTGATGTT
    TTGGGAAATC CCTTTGGCTT GATAAGAGAA TTTTCTGAAG GTGTAGAAGC ATTTTTTTAT
    GAACCTTACC AGGGAGCCAT CCAGGGTCCT GAAGAGTTTG TGGAAGGAAT GGCACTAGGA
    CTGAAAGCAC TAGTTGGTGG AGCTGTTGGT GGACTAGCTG GTGCTGCCTC CAAAATCACC
    AGTGCTATGG CTAAAGGGGT GGCAGCTATG ACTATGGATG AAGACTACCA ACAGAAGAGA
    AGAGAAGCCA TGAATAAGCA ACCAGCTGGT CTTAGGGAAG GCATCACTCG TGGAGGAAAA
    GGCCTAGTTT CTGGTTTTGT AAGTGGCATA ACAGGAATTG TTACAAAACC AATCAAAGGA
    GCTCAGAAAG AAGGAGCAAC TGGTTTCTTT AAAGGTGTTG GGAAAGGTTT AGTTGGAGCA
    GTGACAAGGC CAACTGGAGG CATCATAGAC ATGGCTAGCA GTACATTTCA GGGAATAAAA
    AGAGCTACAG AGACCTCTGA TGAAGTGGAG AGTCTGCGAC CCCCTAGATT CTTTAATGAA
    GATGGAGTTA TCAGACCTTA CAGATTGAGG GATGGTACTG GAAATCAAAT GTTACAGAAA
    ATTCAGGTCT ACAGGGAGTG GATTGTGACT CACAGTAGAA GTAGTGATGA TAATGATGAC
    GATGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320026.1
    CDS21..3869
    Translation

    Target ORF information:

    RefSeq Version XM_015464540.1
    Organism Bos taurus(cattle)
    Definition Bos taurus vacuolar protein sorting 13 homolog A (S. cerevisiae) (VPS13A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAAAAGA AAGCCAAAAA GGCCATTGTT GAGTCAGGTA CTGAAGAAGA AAATTACAGA 
    GTACCAGAAT ACAAAACTGT CATCACTTTC TATTCGAAAG ACCAGCTAAA CATTACATTA
    TCTAAATGTG GCCTTATAAT GTTAAATAAT TTAGCTGAGG CATTTACAGA AGCTGCCACT
    GGATCTTCAG CTACCTTCAT AAGGGATCTG GCACCTTTTA TGATTTTAAA TTCTCTTGGA
    CTTAGTATTT CTGTTTCACC AAGTGAATCT TTTAGTGTAC TCAACGTACC TATGGCAAAA
    TCATATGTAT TGAAAAATGA GGAGAGTTTA AGTATGGATT ATATGCGAAC TAAAGACAAT
    GATCATTTCA ATGCAATGAC CAGCCTAAGC AGCAAACACT TCTTCATTCT TCTTACCCCT
    GCTAACCACT CTACTGCTGA TAAAATCCCT TTAACAAAAG TGGGACGACG ACTGTACACT
    GTAAGACACA GAGAGTCTGG AGTTGAAAGA TCAATTGTTT GTCAGATTGA TGCAGTGGAA
    GGAAGTAAGA AGGTAACAGT TCGCTCTCCA GTGCAGATTA GAAATCATTT TTCAATACCA
    CTTTCTGTTT ATGGAGGAGA CACGTTATTG GGAACGGCCT CACCTGGAAG TGAATTTAAC
    ATACCATTAG CATCTTACCG ATCACTACTT TCTCTGAAGC CAGAAGATGC AGACTATCAG
    ATGTGTGAAG GAATTGACTT TGAAGAAATT ATAAAACAAG ATGGTACTTC TTTCAAGAAA
    AAATGTAGAC CTATAAACCC TTCCAGGAAG CCATTTATCA TTAATATAGT CCCAGAAAAA
    GATAATTTGA CGTATATGTC AGTGTATTCA GAAGACCGTT GGGACTTACC ATATATAATA
    CATTTGTGGC CACCTATTCT ACTCCGAAAT CTTCTTCCTT ACAAAATTGC TTATTACATA
    GAGGGGATTG AACATAGAGT TTTTACTCTC CATGAAGGCC GTTCAGCCCA GCTTTGTACT
    GTACAGTTGG ATAAAGCCAG GCTACATTTA AAATTACTTG ACTATCTTAA TCATGATTGG
    AAAAGTGAAT ATCATATAAA ACCTAATCAA CAAGATATTA GTTTCATCAG TTTTACTTGT
    GTTACAGAAA TAGAAAAGAC TGATTTAGAT ATTGCTGTCC ATGTGACTTA TAATACTGGC
    CAGACTATTA TGGCATTTCA TAGTCCTTAT TGGATGGTTA ATAAAACTGG TCGAATGTTG
    CAGTATAAAG CAGATGGAAT TCATCGAAAG CATCCACCTA ATTATAAAAA GCCAGTTCTC
    TTTTCATTTC AGCCAAAGCA CTTTTTCAAT AACAATAAGG TTCAGCTTAT GGTAACTGAT
    AGTGAATTGT CAGATCAATT TTCAATTGAT ACAGTTGGTA GTCATGGAGC GGTTAAATGT
    AAAGGCCCAA AAATGGAATA TCAAGTTGGT GTCACTATAG ACCTTAGCAG TTTTAACATT
    ACCAGAATTG TCACATTTAC CCCCTTTTTT TTGATTAAAA ACAAAAGCAA ATATCATATA
    TCAGTGGCTG AAGAGGGAAA TGATAAATGG CTCGCTCTTG ATTTGGAGCA GTGTGTCCCC
    TTTTGGCCTG AGGATGCTTC TAATAAACTT CTTATTCGAG TTGAAAGAAG TGAAAGCCCT
    CCCCATAAGA TACATTTTAA TAAGGAAGAA AGTTGTATTT TACTGCGTCT AGACAATGAG
    CTTGGAGGTA TTATAGCAGA AGTTAATTTG GCTGAGCATT CTACAGTTAT TACATTTTCA
    GATTATCGTG ATGGAGCCGC TACATTTCTG TTAATAAATC ACACCAAGAA TGACATTGTT
    CAGTATAAGC AAAGTTCCCT CAGTGAAATA GAAGATTCCC TCCCTCCTGG AAAAGCAGTA
    TTTTATACAT GGGCTGATCC AGTGGGCTCT AGAAAGCTGC AGTGGAAATG TGGAAAAAGC
    CACGGTGAAG TAACACAAAA GGATGATATG ATGACACCTA TAAATTTGGG GAAAACGACC
    ATTTATTTAG TTTCATTTTT TGAAGGCTTA CAACGCATAA TTTTATTCAC TGAAGATCCA
    AGAGTATTTA AAGTGACATA TGAAAGTGAG AAAGCAGAGT TAGCAGAGCA AGAAATTGTG
    TTGGCGTTAC AAGATGTTGG GATTTCTCTT GTCAACAATT ATACAAAGCA AGAAGTAGCC
    TATATTGGCA TTACAAGTTC TGATGTGGTT TGGGAGACAA AGCCCAGGAA GAAGGCAAGA
    TGGAAGCCAA TGAGTGTAAA GCACACTGAG AAGTTAGAGA AGGAATTTAA GGAATATGTT
    GAATCTTCAC CGTCAGAAGA TAAGGTTATT GAGTTGGACA CCAACATTCT GGTTCGCTTC
    ACACCTGGTG CTTCTAACAT GAAAATTCTG CAGCCACATG TAATAGCTAT AAGAAGAAAT
    TATCTTCCAG CATTAAAAGT AGAATATAGC ACATCTGCAC ATCAGTCATC GTTTAGAATT
    CAAATTTATA GAATACAGAT CCAAAACCAA ATACATGGTG CTATATTTCC ATTCGTGTTT
    TATCCCATTA AACCGCCCAA GTCCGTCACC TTGGATTCAG CACCGAAACC CTTTACTGAT
    GTCAGTATTG TCATGAGATC TGCAGGACAT TCGCAGATAT CACGTATTAA GTATTTCAAA
    GTGTTGATCC AAGAAATGGA TCTCAGGTTA GATCTTGGAT TTGTCTATGC TGTATCAGAA
    CTTATGACAC CAGCTGAAGT CACTGAAAAA ACAGAGGTTG AGCTTTTTCA TAAAGACATA
    GAAGCTTTCC AAGAAGAATA TAAAATAGTC TCATTAGTAG ATTCATCACA AGTCAGTCTC
    TATGAATACT TTCATATATC TCCTATCAAG TTGCATTTAA GTGTTTCACT GAGTTCAGGT
    GGTGAAGAAC CGAAAGATTC AGAACAGCAT GAAGGACTGA TGGCAATTCG TTCTTTACAT
    CTTTTGCTGA AGAGTATTGG TGCCACTCTC ACAGATGTAC AAGATGTAGT TTTTAAGCTA
    GCATTTTTTG AACTCAATTA TCAGTTCCAT ACAACAGCTG AACTACAGTC TGAAGTAATA
    AGACACTATT CAAAGCAGGC CATTAAACAG ATGTACGTAC TCATTCTTGG ACTTGATGTT
    TTGGGAAATC CCTTTGGCTT GATAAGAGAA TTTTCTGAAG GTGTAGAAGC ATTTTTTTAT
    GAACCTTACC AGGGAGCCAT CCAGGGTCCT GAAGAGTTTG TGGAAGGAAT GGCACTAGGA
    CTGAAAGCAC TAGTTGGTGG AGCTGTTGGT GGACTAGCTG GTGCTGCCTC CAAAATCACC
    AGTGCTATGG CTAAAGGGGT GGCAGCTATG ACTATGGATG AAGACTACCA ACAGAAGAGA
    AGAGAAGCCA TGAATAAGCA ACCAGCTGGT CTTAGGGAAG GCATCACTCG TGGAGGAAAA
    GGCCTAGTTT CTGGTTTTGT AAGTGGCATA ACAGGAATTG TTACAAAACC AATCAAAGGA
    GCTCAGAAAG AAGGAGCAAC TGGTTTCTTT AAAGGTGTTG GGAAAGGTTT AGTTGGAGCA
    GTGACAAGGC CAACTGGAGG CATCATAGAC ATGGCTAGCA GTACATTTCA GGGAATAAAA
    AGAGCTACAG AGACCTCTGA TGAAGTGGAG AGTCTGCGAC CCCCTAGATT CTTTAATGAA
    GATGGAGTTA TCAGACCTTA CAGATTGAGG GATGGTACTG GAAATCAAAT GTTACAGAAA
    ATTCAGGTCT ACAGGGAGTG GATTGTGACT CACAGTAGAA GTAGTGATGA TAATGATGAC
    GATGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50054
    Clone ID Related Accession (Same CDS sequence) XM_015464540.1 , XM_010807909.2
    Accession Version XM_010807909.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product vacuolar protein sorting-associated protein 13A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807909.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAAAAGA AAGCCAAAAA GGCCATTGTT GAGTCAGGTA CTGAAGAAGA AAATTACAGA 
    GTACCAGAAT ACAAAACTGT CATCACTTTC TATTCGAAAG ACCAGCTAAA CATTACATTA
    TCTAAATGTG GCCTTATAAT GTTAAATAAT TTAGCTGAGG CATTTACAGA AGCTGCCACT
    GGATCTTCAG CTACCTTCAT AAGGGATCTG GCACCTTTTA TGATTTTAAA TTCTCTTGGA
    CTTAGTATTT CTGTTTCACC AAGTGAATCT TTTAGTGTAC TCAACGTACC TATGGCAAAA
    TCATATGTAT TGAAAAATGA GGAGAGTTTA AGTATGGATT ATATGCGAAC TAAAGACAAT
    GATCATTTCA ATGCAATGAC CAGCCTAAGC AGCAAACACT TCTTCATTCT TCTTACCCCT
    GCTAACCACT CTACTGCTGA TAAAATCCCT TTAACAAAAG TGGGACGACG ACTGTACACT
    GTAAGACACA GAGAGTCTGG AGTTGAAAGA TCAATTGTTT GTCAGATTGA TGCAGTGGAA
    GGAAGTAAGA AGGTAACAGT TCGCTCTCCA GTGCAGATTA GAAATCATTT TTCAATACCA
    CTTTCTGTTT ATGGAGGAGA CACGTTATTG GGAACGGCCT CACCTGGAAG TGAATTTAAC
    ATACCATTAG CATCTTACCG ATCACTACTT TCTCTGAAGC CAGAAGATGC AGACTATCAG
    ATGTGTGAAG GAATTGACTT TGAAGAAATT ATAAAACAAG ATGGTACTTC TTTCAAGAAA
    AAATGTAGAC CTATAAACCC TTCCAGGAAG CCATTTATCA TTAATATAGT CCCAGAAAAA
    GATAATTTGA CGTATATGTC AGTGTATTCA GAAGACCGTT GGGACTTACC ATATATAATA
    CATTTGTGGC CACCTATTCT ACTCCGAAAT CTTCTTCCTT ACAAAATTGC TTATTACATA
    GAGGGGATTG AACATAGAGT TTTTACTCTC CATGAAGGCC GTTCAGCCCA GCTTTGTACT
    GTACAGTTGG ATAAAGCCAG GCTACATTTA AAATTACTTG ACTATCTTAA TCATGATTGG
    AAAAGTGAAT ATCATATAAA ACCTAATCAA CAAGATATTA GTTTCATCAG TTTTACTTGT
    GTTACAGAAA TAGAAAAGAC TGATTTAGAT ATTGCTGTCC ATGTGACTTA TAATACTGGC
    CAGACTATTA TGGCATTTCA TAGTCCTTAT TGGATGGTTA ATAAAACTGG TCGAATGTTG
    CAGTATAAAG CAGATGGAAT TCATCGAAAG CATCCACCTA ATTATAAAAA GCCAGTTCTC
    TTTTCATTTC AGCCAAAGCA CTTTTTCAAT AACAATAAGG TTCAGCTTAT GGTAACTGAT
    AGTGAATTGT CAGATCAATT TTCAATTGAT ACAGTTGGTA GTCATGGAGC GGTTAAATGT
    AAAGGCCCAA AAATGGAATA TCAAGTTGGT GTCACTATAG ACCTTAGCAG TTTTAACATT
    ACCAGAATTG TCACATTTAC CCCCTTTTTT TTGATTAAAA ACAAAAGCAA ATATCATATA
    TCAGTGGCTG AAGAGGGAAA TGATAAATGG CTCGCTCTTG ATTTGGAGCA GTGTGTCCCC
    TTTTGGCCTG AGGATGCTTC TAATAAACTT CTTATTCGAG TTGAAAGAAG TGAAAGCCCT
    CCCCATAAGA TACATTTTAA TAAGGAAGAA AGTTGTATTT TACTGCGTCT AGACAATGAG
    CTTGGAGGTA TTATAGCAGA AGTTAATTTG GCTGAGCATT CTACAGTTAT TACATTTTCA
    GATTATCGTG ATGGAGCCGC TACATTTCTG TTAATAAATC ACACCAAGAA TGACATTGTT
    CAGTATAAGC AAAGTTCCCT CAGTGAAATA GAAGATTCCC TCCCTCCTGG AAAAGCAGTA
    TTTTATACAT GGGCTGATCC AGTGGGCTCT AGAAAGCTGC AGTGGAAATG TGGAAAAAGC
    CACGGTGAAG TAACACAAAA GGATGATATG ATGACACCTA TAAATTTGGG GAAAACGACC
    ATTTATTTAG TTTCATTTTT TGAAGGCTTA CAACGCATAA TTTTATTCAC TGAAGATCCA
    AGAGTATTTA AAGTGACATA TGAAAGTGAG AAAGCAGAGT TAGCAGAGCA AGAAATTGTG
    TTGGCGTTAC AAGATGTTGG GATTTCTCTT GTCAACAATT ATACAAAGCA AGAAGTAGCC
    TATATTGGCA TTACAAGTTC TGATGTGGTT TGGGAGACAA AGCCCAGGAA GAAGGCAAGA
    TGGAAGCCAA TGAGTGTAAA GCACACTGAG AAGTTAGAGA AGGAATTTAA GGAATATGTT
    GAATCTTCAC CGTCAGAAGA TAAGGTTATT GAGTTGGACA CCAACATTCT GGTTCGCTTC
    ACACCTGGTG CTTCTAACAT GAAAATTCTG CAGCCACATG TAATAGCTAT AAGAAGAAAT
    TATCTTCCAG CATTAAAAGT AGAATATAGC ACATCTGCAC ATCAGTCATC GTTTAGAATT
    CAAATTTATA GAATACAGAT CCAAAACCAA ATACATGGTG CTATATTTCC ATTCGTGTTT
    TATCCCATTA AACCGCCCAA GTCCGTCACC TTGGATTCAG CACCGAAACC CTTTACTGAT
    GTCAGTATTG TCATGAGATC TGCAGGACAT TCGCAGATAT CACGTATTAA GTATTTCAAA
    GTGTTGATCC AAGAAATGGA TCTCAGGTTA GATCTTGGAT TTGTCTATGC TGTATCAGAA
    CTTATGACAC CAGCTGAAGT CACTGAAAAA ACAGAGGTTG AGCTTTTTCA TAAAGACATA
    GAAGCTTTCC AAGAAGAATA TAAAATAGTC TCATTAGTAG ATTCATCACA AGTCAGTCTC
    TATGAATACT TTCATATATC TCCTATCAAG TTGCATTTAA GTGTTTCACT GAGTTCAGGT
    GGTGAAGAAC CGAAAGATTC AGAACAGCAT GAAGGACTGA TGGCAATTCG TTCTTTACAT
    CTTTTGCTGA AGAGTATTGG TGCCACTCTC ACAGATGTAC AAGATGTAGT TTTTAAGCTA
    GCATTTTTTG AACTCAATTA TCAGTTCCAT ACAACAGCTG AACTACAGTC TGAAGTAATA
    AGACACTATT CAAAGCAGGC CATTAAACAG ATGTACGTAC TCATTCTTGG ACTTGATGTT
    TTGGGAAATC CCTTTGGCTT GATAAGAGAA TTTTCTGAAG GTGTAGAAGC ATTTTTTTAT
    GAACCTTACC AGGGAGCCAT CCAGGGTCCT GAAGAGTTTG TGGAAGGAAT GGCACTAGGA
    CTGAAAGCAC TAGTTGGTGG AGCTGTTGGT GGACTAGCTG GTGCTGCCTC CAAAATCACC
    AGTGCTATGG CTAAAGGGGT GGCAGCTATG ACTATGGATG AAGACTACCA ACAGAAGAGA
    AGAGAAGCCA TGAATAAGCA ACCAGCTGGT CTTAGGGAAG GCATCACTCG TGGAGGAAAA
    GGCCTAGTTT CTGGTTTTGT AAGTGGCATA ACAGGAATTG TTACAAAACC AATCAAAGGA
    GCTCAGAAAG AAGGAGCAAC TGGTTTCTTT AAAGGTGTTG GGAAAGGTTT AGTTGGAGCA
    GTGACAAGGC CAACTGGAGG CATCATAGAC ATGGCTAGCA GTACATTTCA GGGAATAAAA
    AGAGCTACAG AGACCTCTGA TGAAGTGGAG AGTCTGCGAC CCCCTAGATT CTTTAATGAA
    GATGGAGTTA TCAGACCTTA CAGATTGAGG GATGGTACTG GAAATCAAAT GTTACAGAAA
    ATTCAGGTCT ACAGGGAGTG GATTGTGACT CACAGTAGAA GTAGTGATGA TAATGATGAC
    GATGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806211.1
    CDS21..3869
    Translation

    Target ORF information:

    RefSeq Version XM_010807909.2
    Organism Bos taurus(cattle)
    Definition Bos taurus vacuolar protein sorting 13 homolog A (S. cerevisiae) (VPS13A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807909.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAAAAGA AAGCCAAAAA GGCCATTGTT GAGTCAGGTA CTGAAGAAGA AAATTACAGA 
    GTACCAGAAT ACAAAACTGT CATCACTTTC TATTCGAAAG ACCAGCTAAA CATTACATTA
    TCTAAATGTG GCCTTATAAT GTTAAATAAT TTAGCTGAGG CATTTACAGA AGCTGCCACT
    GGATCTTCAG CTACCTTCAT AAGGGATCTG GCACCTTTTA TGATTTTAAA TTCTCTTGGA
    CTTAGTATTT CTGTTTCACC AAGTGAATCT TTTAGTGTAC TCAACGTACC TATGGCAAAA
    TCATATGTAT TGAAAAATGA GGAGAGTTTA AGTATGGATT ATATGCGAAC TAAAGACAAT
    GATCATTTCA ATGCAATGAC CAGCCTAAGC AGCAAACACT TCTTCATTCT TCTTACCCCT
    GCTAACCACT CTACTGCTGA TAAAATCCCT TTAACAAAAG TGGGACGACG ACTGTACACT
    GTAAGACACA GAGAGTCTGG AGTTGAAAGA TCAATTGTTT GTCAGATTGA TGCAGTGGAA
    GGAAGTAAGA AGGTAACAGT TCGCTCTCCA GTGCAGATTA GAAATCATTT TTCAATACCA
    CTTTCTGTTT ATGGAGGAGA CACGTTATTG GGAACGGCCT CACCTGGAAG TGAATTTAAC
    ATACCATTAG CATCTTACCG ATCACTACTT TCTCTGAAGC CAGAAGATGC AGACTATCAG
    ATGTGTGAAG GAATTGACTT TGAAGAAATT ATAAAACAAG ATGGTACTTC TTTCAAGAAA
    AAATGTAGAC CTATAAACCC TTCCAGGAAG CCATTTATCA TTAATATAGT CCCAGAAAAA
    GATAATTTGA CGTATATGTC AGTGTATTCA GAAGACCGTT GGGACTTACC ATATATAATA
    CATTTGTGGC CACCTATTCT ACTCCGAAAT CTTCTTCCTT ACAAAATTGC TTATTACATA
    GAGGGGATTG AACATAGAGT TTTTACTCTC CATGAAGGCC GTTCAGCCCA GCTTTGTACT
    GTACAGTTGG ATAAAGCCAG GCTACATTTA AAATTACTTG ACTATCTTAA TCATGATTGG
    AAAAGTGAAT ATCATATAAA ACCTAATCAA CAAGATATTA GTTTCATCAG TTTTACTTGT
    GTTACAGAAA TAGAAAAGAC TGATTTAGAT ATTGCTGTCC ATGTGACTTA TAATACTGGC
    CAGACTATTA TGGCATTTCA TAGTCCTTAT TGGATGGTTA ATAAAACTGG TCGAATGTTG
    CAGTATAAAG CAGATGGAAT TCATCGAAAG CATCCACCTA ATTATAAAAA GCCAGTTCTC
    TTTTCATTTC AGCCAAAGCA CTTTTTCAAT AACAATAAGG TTCAGCTTAT GGTAACTGAT
    AGTGAATTGT CAGATCAATT TTCAATTGAT ACAGTTGGTA GTCATGGAGC GGTTAAATGT
    AAAGGCCCAA AAATGGAATA TCAAGTTGGT GTCACTATAG ACCTTAGCAG TTTTAACATT
    ACCAGAATTG TCACATTTAC CCCCTTTTTT TTGATTAAAA ACAAAAGCAA ATATCATATA
    TCAGTGGCTG AAGAGGGAAA TGATAAATGG CTCGCTCTTG ATTTGGAGCA GTGTGTCCCC
    TTTTGGCCTG AGGATGCTTC TAATAAACTT CTTATTCGAG TTGAAAGAAG TGAAAGCCCT
    CCCCATAAGA TACATTTTAA TAAGGAAGAA AGTTGTATTT TACTGCGTCT AGACAATGAG
    CTTGGAGGTA TTATAGCAGA AGTTAATTTG GCTGAGCATT CTACAGTTAT TACATTTTCA
    GATTATCGTG ATGGAGCCGC TACATTTCTG TTAATAAATC ACACCAAGAA TGACATTGTT
    CAGTATAAGC AAAGTTCCCT CAGTGAAATA GAAGATTCCC TCCCTCCTGG AAAAGCAGTA
    TTTTATACAT GGGCTGATCC AGTGGGCTCT AGAAAGCTGC AGTGGAAATG TGGAAAAAGC
    CACGGTGAAG TAACACAAAA GGATGATATG ATGACACCTA TAAATTTGGG GAAAACGACC
    ATTTATTTAG TTTCATTTTT TGAAGGCTTA CAACGCATAA TTTTATTCAC TGAAGATCCA
    AGAGTATTTA AAGTGACATA TGAAAGTGAG AAAGCAGAGT TAGCAGAGCA AGAAATTGTG
    TTGGCGTTAC AAGATGTTGG GATTTCTCTT GTCAACAATT ATACAAAGCA AGAAGTAGCC
    TATATTGGCA TTACAAGTTC TGATGTGGTT TGGGAGACAA AGCCCAGGAA GAAGGCAAGA
    TGGAAGCCAA TGAGTGTAAA GCACACTGAG AAGTTAGAGA AGGAATTTAA GGAATATGTT
    GAATCTTCAC CGTCAGAAGA TAAGGTTATT GAGTTGGACA CCAACATTCT GGTTCGCTTC
    ACACCTGGTG CTTCTAACAT GAAAATTCTG CAGCCACATG TAATAGCTAT AAGAAGAAAT
    TATCTTCCAG CATTAAAAGT AGAATATAGC ACATCTGCAC ATCAGTCATC GTTTAGAATT
    CAAATTTATA GAATACAGAT CCAAAACCAA ATACATGGTG CTATATTTCC ATTCGTGTTT
    TATCCCATTA AACCGCCCAA GTCCGTCACC TTGGATTCAG CACCGAAACC CTTTACTGAT
    GTCAGTATTG TCATGAGATC TGCAGGACAT TCGCAGATAT CACGTATTAA GTATTTCAAA
    GTGTTGATCC AAGAAATGGA TCTCAGGTTA GATCTTGGAT TTGTCTATGC TGTATCAGAA
    CTTATGACAC CAGCTGAAGT CACTGAAAAA ACAGAGGTTG AGCTTTTTCA TAAAGACATA
    GAAGCTTTCC AAGAAGAATA TAAAATAGTC TCATTAGTAG ATTCATCACA AGTCAGTCTC
    TATGAATACT TTCATATATC TCCTATCAAG TTGCATTTAA GTGTTTCACT GAGTTCAGGT
    GGTGAAGAAC CGAAAGATTC AGAACAGCAT GAAGGACTGA TGGCAATTCG TTCTTTACAT
    CTTTTGCTGA AGAGTATTGG TGCCACTCTC ACAGATGTAC AAGATGTAGT TTTTAAGCTA
    GCATTTTTTG AACTCAATTA TCAGTTCCAT ACAACAGCTG AACTACAGTC TGAAGTAATA
    AGACACTATT CAAAGCAGGC CATTAAACAG ATGTACGTAC TCATTCTTGG ACTTGATGTT
    TTGGGAAATC CCTTTGGCTT GATAAGAGAA TTTTCTGAAG GTGTAGAAGC ATTTTTTTAT
    GAACCTTACC AGGGAGCCAT CCAGGGTCCT GAAGAGTTTG TGGAAGGAAT GGCACTAGGA
    CTGAAAGCAC TAGTTGGTGG AGCTGTTGGT GGACTAGCTG GTGCTGCCTC CAAAATCACC
    AGTGCTATGG CTAAAGGGGT GGCAGCTATG ACTATGGATG AAGACTACCA ACAGAAGAGA
    AGAGAAGCCA TGAATAAGCA ACCAGCTGGT CTTAGGGAAG GCATCACTCG TGGAGGAAAA
    GGCCTAGTTT CTGGTTTTGT AAGTGGCATA ACAGGAATTG TTACAAAACC AATCAAAGGA
    GCTCAGAAAG AAGGAGCAAC TGGTTTCTTT AAAGGTGTTG GGAAAGGTTT AGTTGGAGCA
    GTGACAAGGC CAACTGGAGG CATCATAGAC ATGGCTAGCA GTACATTTCA GGGAATAAAA
    AGAGCTACAG AGACCTCTGA TGAAGTGGAG AGTCTGCGAC CCCCTAGATT CTTTAATGAA
    GATGGAGTTA TCAGACCTTA CAGATTGAGG GATGGTACTG GAAATCAAAT GTTACAGAAA
    ATTCAGGTCT ACAGGGAGTG GATTGTGACT CACAGTAGAA GTAGTGATGA TAATGATGAC
    GATGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50055
    Clone ID Related Accession (Same CDS sequence) XM_010807910.2 , XM_015464541.1
    Accession Version XM_010807910.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product vacuolar protein sorting-associated protein 13A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807910.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAAAAGA AAGCCAAAAA GGCCATTGTT GAGTCAGGTA CTGAAGAAGA AAATTACAGA 
    GTACCAGAAT ACAAAACTGT CATCACTTTC TATTCGAAAG ACCAGCTAAA CATTACATTA
    TCTAAATGTG GCCTTATAAT GTTAAATAAT TTAGCTGAGG CATTTACAGA AGCTGCCACT
    GGATCTTCAG CTACCTTCAT AAGGGATCTG GCACCTTTTA TGATTTTAAA TTCTCTTGGA
    CTTAGTATTT CTGTTTCACC AAGTGAATCT TTTAGTGTAC TCAACGTACC TATGGCAAAA
    TCATATGTAT TGAAAAATGA GGAGAGTTTA AGTATGGATT ATATGCGAAC TAAAGACAAT
    GATCATTTCA ATGCAATGAC CAGCCTAAGC AGCAAACACT TCTTCATTCT TCTTACCCCT
    GCTAACCACT CTACTGCTGA TAAAATCCCT TTAACAAAAG TGGGACGACG ACTGTACACT
    GTAAGACACA GAGAGTCTGG AGTTGAAAGA TCAATTGTTT GTCAGATTGA TGCAGTGGAA
    GGAAGTAAGA AGGTAACAGT TCGCTCTCCA GTGCAGATTA GAAATCATTT TTCAATACCA
    CTTTCTGTTT ATGGAGGAGA CACGTTATTG GGAACGGCCT CACCTGGAAG TGAATTTAAC
    ATACCATTAG CATCTTACCG ATCACTACTT TCTCTGAAGC CAGAAGATGC AGACTATCAG
    ATGTGTGAAG GAATTGACTT TGAAGAAATT ATAAAACAAG ATGGTACTTC TTTCAAGAAA
    AAATGTAGAC CTATAAACCC TTCCAGGAAG CCATTTATCA TTAATATAGT CCCAGAAAAA
    GATAATTTGA CGTATATGTC AGTGTATTCA GAAGACCGTT GGGACTTACC ATATATAATA
    CATTTGTGGC CACCTATTCT ACTCCGAAAT CTTCTTCCTT ACAAAATTGC TTATTACATA
    GAGGGGATTG AACATAGAGT TTTTACTCTC CATGAAGGCC GTTCAGCCCA GCTTTGTACT
    GTACAGTTGG ATAAAGCCAG GCTACATTTA AAATTACTTG ACTATCTTAA TCATGATTGG
    AAAAGTGAAT ATCATATAAA ACCTAATCAA CAAGATATTA GTTTCATCAG TTTTACTTGT
    GTTACAGAAA TAGAAAAGAC TGATTTAGAT ATTGCTGTCC ATGTGACTTA TAATACTGGC
    CAGACTATTA TGGCATTTCA TAGTCCTTAT TGGATGGTTA ATAAAACTGG TCGAATGTTG
    CAGTATAAAG CAGATGGAAT TCATCGAAAG CATCCACCTA ATTATAAAAA GCCAGTTCTC
    TTTTCATTTC AGCCAAAGCA CTTTTTCAAT AACAATAAGG TTCAGCTTAT GGTAACTGAT
    AGTGAATTGT CAGATCAATT TTCAATTGAT ACAGTTGGTA GTCATGGAGC GGTTAAATGT
    AAAGGCCCAA AAATGGAATA TCAAGTTGGT GTCACTATAG ACCTTAGCAG TTTTAACATT
    ACCAGAATTG TCACATTTAC CCCCTTTTTT TTGATTAAAA ACAAAAGCAA ATATCATATA
    TCAGTGGCTG AAGAGGGAAA TGATAAATGG CTCGCTCTTG ATTTGGAGCA GTGTGTCCCC
    TTTTGGCCTG AGGATGCTTC TAATAAACTT CTTATTCGAG TTGAAAGAAG TGAAAGCCCT
    CCCCATAAGA TACATTTTAA TAAGGAAGAA AGTTGTATTT TACTGCGTCT AGACAATGAG
    CTTGGAGGTA TTATAGCAGA AGTTAATTTG GCTGAGCATT CTACAGTTAT TACATTTTCA
    GATTATCGTG ATGGAGCCGC TACATTTCTG TTAATAAATC ACACCAAGAA TGACATTGTT
    CAGTATAAGC AAAGTTCCCT CAGTGAAATA GAAGATTCCC TCCCTCCTGG AAAAGCAGTA
    TTTTATACAT GGGCTGATCC AGTGGGCTCT AGAAAGCTGC AGTGGAAATG TGGAAAAAGC
    CACGGTGAAG TAACACAAAA GGATGATATG ATGACACCTA TAAATTTGGG GAAAACGACC
    ATTTATTTAG TTTCATTTTT TGAAGGCTTA CAACGCATAA TTTTATTCAC TGAAGATCCA
    AGAGTATTTA AAGTGACATA TGAAAGTGAG AAAGCAGAGT TAGCAGAGCA AGAAATTGTG
    TTGGCGTTAC AAGATGTTGG GATTTCTCTT GTCAACAATT ATACAAAGCA AGAAGTAGCC
    TATATTGGCA TTACAAGTTC TGATGTGGTT TGGGAGACAA AGCCCAGGAA GAAGGCAAGA
    TGGAAGCCAA TGAGTGTAAA GCACACTGAG AAGTTAGAGA AGGAATTTAA GGAATATGTT
    GAATCTTCAC CGTCAGAAGA TAAGGTTATT GAGTTGGACA CCAACATTCT GGTTCGCTTC
    ACACCTGGTG CTTCTAACAT GAAAATTCTG CAGCCACATG TAATAGCTAT AAGAAGAAAT
    TATCTTCCAG CATTAAAAGT AGAATATAGC ACATCTGCAC ATCAGTCATC GTTTAGAATT
    CAAATTTATA GAATACAGAT CCAAAACCAA ATACATGGTG CTATATTTCC ATTCGTGTTT
    TATCCCATTA AACCGCCCAA GTCCGTCACC TTGGATTCAG CACCGAAACC CTTTACTGAT
    GTCAGTATTG TCATGAGATC TGCAGGACAT TCGCAGATAT CACGTATTAA GTATTTCAAA
    GTGTTGATCC AAGAAATGGA TCTCAGGTTA GATCTTGGAT TTGTCTATGC TGTATCAGAA
    CTTATGACAC CAGCTGAAGT CACTGAAAAA ACAGAGGTTG AGCTTTTTCA TAAAGACATA
    GAAGCTTTCC AAGAAGAATA TAAAATAGTC TCATTAGTAG ATTCATCACA AGTCAGTCTC
    TATGAATACT TTCATATATC TCCTATCAAG TTGCATTTAA GTGTTTCACT GAGTTCAGGT
    GGTGAAGAAC CGAAAGATTC AGAACAGCAT GAAGGACTGA TGGCAATTCG TTCTTTACAT
    CTTTTGCTGA AGAGTATTGG TGCCACTCTC ACAGATGTAC AAGATGTAGT TTTTAAGCTA
    GCATTTTTTG AACTCAATTA TCAGTTCCAT ACAACAGCTG AACTACAGTC TGAAGTAATA
    AGACACTATT CAAAGCAGGC CATTAAACAG ATGTACGTAC TCATTCTTGG ACTTGATGTT
    TTGGGAAATC CCTTTGGCTT GATAAGAGAA TTTTCTGAAG GTGTAGAAGC ATTTTTTTAT
    GAACCTTACC AGGGAGCCAT CCAGGGTCCT GAAGAGTTTG TGGAAGGAAT GGCACTAGGA
    CTGAAAGCAC TAGTTGGTGG AGCTGTTGGT GGACTAGCTG GTGCTGCCTC CAAAATCACC
    AGTGCTATGG CTAAAGGGGT GGCAGCTATG ACTATGGATG AAGACTACCA ACAGAAGAGA
    AGAGAAGCCA TGAATAAGCA ACCAGCTGGT CTTAGGGAAG GCATCACTCG TGGAGGAAAA
    GGCCTAGTTT CTGGTTTTGT AAGTGGCATA ACAGGAATTG TTACAAAACC AATCAAAGGA
    GCTCAGAAAG AAGGAGCAAC TGGTTTCTTT AAAGGTGTTG GGAAAGGTTT AGTTGGAGCA
    GTGACAAGGC CAACTGGAGG CATCATAGAC ATGGCTAGCA GTACATTTCA GGGAATAAAA
    AGAGCTACAG AGACCTCTGA TGAAGTGGAG AGTCTGCGAC CCCCTAGATT CTTTAATGAA
    GATGGAGTTA TCAGACCTTA CAGATTGAGG GATGGTACTG GAAATCAAAT GTTACAGGCA
    TCAAAAAGTT TGATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806212.1
    CDS21..3818
    Translation

    Target ORF information:

    RefSeq Version XM_010807910.2
    Organism Bos taurus(cattle)
    Definition Bos taurus vacuolar protein sorting 13 homolog A (S. cerevisiae) (VPS13A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807910.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAAAAGA AAGCCAAAAA GGCCATTGTT GAGTCAGGTA CTGAAGAAGA AAATTACAGA 
    GTACCAGAAT ACAAAACTGT CATCACTTTC TATTCGAAAG ACCAGCTAAA CATTACATTA
    TCTAAATGTG GCCTTATAAT GTTAAATAAT TTAGCTGAGG CATTTACAGA AGCTGCCACT
    GGATCTTCAG CTACCTTCAT AAGGGATCTG GCACCTTTTA TGATTTTAAA TTCTCTTGGA
    CTTAGTATTT CTGTTTCACC AAGTGAATCT TTTAGTGTAC TCAACGTACC TATGGCAAAA
    TCATATGTAT TGAAAAATGA GGAGAGTTTA AGTATGGATT ATATGCGAAC TAAAGACAAT
    GATCATTTCA ATGCAATGAC CAGCCTAAGC AGCAAACACT TCTTCATTCT TCTTACCCCT
    GCTAACCACT CTACTGCTGA TAAAATCCCT TTAACAAAAG TGGGACGACG ACTGTACACT
    GTAAGACACA GAGAGTCTGG AGTTGAAAGA TCAATTGTTT GTCAGATTGA TGCAGTGGAA
    GGAAGTAAGA AGGTAACAGT TCGCTCTCCA GTGCAGATTA GAAATCATTT TTCAATACCA
    CTTTCTGTTT ATGGAGGAGA CACGTTATTG GGAACGGCCT CACCTGGAAG TGAATTTAAC
    ATACCATTAG CATCTTACCG ATCACTACTT TCTCTGAAGC CAGAAGATGC AGACTATCAG
    ATGTGTGAAG GAATTGACTT TGAAGAAATT ATAAAACAAG ATGGTACTTC TTTCAAGAAA
    AAATGTAGAC CTATAAACCC TTCCAGGAAG CCATTTATCA TTAATATAGT CCCAGAAAAA
    GATAATTTGA CGTATATGTC AGTGTATTCA GAAGACCGTT GGGACTTACC ATATATAATA
    CATTTGTGGC CACCTATTCT ACTCCGAAAT CTTCTTCCTT ACAAAATTGC TTATTACATA
    GAGGGGATTG AACATAGAGT TTTTACTCTC CATGAAGGCC GTTCAGCCCA GCTTTGTACT
    GTACAGTTGG ATAAAGCCAG GCTACATTTA AAATTACTTG ACTATCTTAA TCATGATTGG
    AAAAGTGAAT ATCATATAAA ACCTAATCAA CAAGATATTA GTTTCATCAG TTTTACTTGT
    GTTACAGAAA TAGAAAAGAC TGATTTAGAT ATTGCTGTCC ATGTGACTTA TAATACTGGC
    CAGACTATTA TGGCATTTCA TAGTCCTTAT TGGATGGTTA ATAAAACTGG TCGAATGTTG
    CAGTATAAAG CAGATGGAAT TCATCGAAAG CATCCACCTA ATTATAAAAA GCCAGTTCTC
    TTTTCATTTC AGCCAAAGCA CTTTTTCAAT AACAATAAGG TTCAGCTTAT GGTAACTGAT
    AGTGAATTGT CAGATCAATT TTCAATTGAT ACAGTTGGTA GTCATGGAGC GGTTAAATGT
    AAAGGCCCAA AAATGGAATA TCAAGTTGGT GTCACTATAG ACCTTAGCAG TTTTAACATT
    ACCAGAATTG TCACATTTAC CCCCTTTTTT TTGATTAAAA ACAAAAGCAA ATATCATATA
    TCAGTGGCTG AAGAGGGAAA TGATAAATGG CTCGCTCTTG ATTTGGAGCA GTGTGTCCCC
    TTTTGGCCTG AGGATGCTTC TAATAAACTT CTTATTCGAG TTGAAAGAAG TGAAAGCCCT
    CCCCATAAGA TACATTTTAA TAAGGAAGAA AGTTGTATTT TACTGCGTCT AGACAATGAG
    CTTGGAGGTA TTATAGCAGA AGTTAATTTG GCTGAGCATT CTACAGTTAT TACATTTTCA
    GATTATCGTG ATGGAGCCGC TACATTTCTG TTAATAAATC ACACCAAGAA TGACATTGTT
    CAGTATAAGC AAAGTTCCCT CAGTGAAATA GAAGATTCCC TCCCTCCTGG AAAAGCAGTA
    TTTTATACAT GGGCTGATCC AGTGGGCTCT AGAAAGCTGC AGTGGAAATG TGGAAAAAGC
    CACGGTGAAG TAACACAAAA GGATGATATG ATGACACCTA TAAATTTGGG GAAAACGACC
    ATTTATTTAG TTTCATTTTT TGAAGGCTTA CAACGCATAA TTTTATTCAC TGAAGATCCA
    AGAGTATTTA AAGTGACATA TGAAAGTGAG AAAGCAGAGT TAGCAGAGCA AGAAATTGTG
    TTGGCGTTAC AAGATGTTGG GATTTCTCTT GTCAACAATT ATACAAAGCA AGAAGTAGCC
    TATATTGGCA TTACAAGTTC TGATGTGGTT TGGGAGACAA AGCCCAGGAA GAAGGCAAGA
    TGGAAGCCAA TGAGTGTAAA GCACACTGAG AAGTTAGAGA AGGAATTTAA GGAATATGTT
    GAATCTTCAC CGTCAGAAGA TAAGGTTATT GAGTTGGACA CCAACATTCT GGTTCGCTTC
    ACACCTGGTG CTTCTAACAT GAAAATTCTG CAGCCACATG TAATAGCTAT AAGAAGAAAT
    TATCTTCCAG CATTAAAAGT AGAATATAGC ACATCTGCAC ATCAGTCATC GTTTAGAATT
    CAAATTTATA GAATACAGAT CCAAAACCAA ATACATGGTG CTATATTTCC ATTCGTGTTT
    TATCCCATTA AACCGCCCAA GTCCGTCACC TTGGATTCAG CACCGAAACC CTTTACTGAT
    GTCAGTATTG TCATGAGATC TGCAGGACAT TCGCAGATAT CACGTATTAA GTATTTCAAA
    GTGTTGATCC AAGAAATGGA TCTCAGGTTA GATCTTGGAT TTGTCTATGC TGTATCAGAA
    CTTATGACAC CAGCTGAAGT CACTGAAAAA ACAGAGGTTG AGCTTTTTCA TAAAGACATA
    GAAGCTTTCC AAGAAGAATA TAAAATAGTC TCATTAGTAG ATTCATCACA AGTCAGTCTC
    TATGAATACT TTCATATATC TCCTATCAAG TTGCATTTAA GTGTTTCACT GAGTTCAGGT
    GGTGAAGAAC CGAAAGATTC AGAACAGCAT GAAGGACTGA TGGCAATTCG TTCTTTACAT
    CTTTTGCTGA AGAGTATTGG TGCCACTCTC ACAGATGTAC AAGATGTAGT TTTTAAGCTA
    GCATTTTTTG AACTCAATTA TCAGTTCCAT ACAACAGCTG AACTACAGTC TGAAGTAATA
    AGACACTATT CAAAGCAGGC CATTAAACAG ATGTACGTAC TCATTCTTGG ACTTGATGTT
    TTGGGAAATC CCTTTGGCTT GATAAGAGAA TTTTCTGAAG GTGTAGAAGC ATTTTTTTAT
    GAACCTTACC AGGGAGCCAT CCAGGGTCCT GAAGAGTTTG TGGAAGGAAT GGCACTAGGA
    CTGAAAGCAC TAGTTGGTGG AGCTGTTGGT GGACTAGCTG GTGCTGCCTC CAAAATCACC
    AGTGCTATGG CTAAAGGGGT GGCAGCTATG ACTATGGATG AAGACTACCA ACAGAAGAGA
    AGAGAAGCCA TGAATAAGCA ACCAGCTGGT CTTAGGGAAG GCATCACTCG TGGAGGAAAA
    GGCCTAGTTT CTGGTTTTGT AAGTGGCATA ACAGGAATTG TTACAAAACC AATCAAAGGA
    GCTCAGAAAG AAGGAGCAAC TGGTTTCTTT AAAGGTGTTG GGAAAGGTTT AGTTGGAGCA
    GTGACAAGGC CAACTGGAGG CATCATAGAC ATGGCTAGCA GTACATTTCA GGGAATAAAA
    AGAGCTACAG AGACCTCTGA TGAAGTGGAG AGTCTGCGAC CCCCTAGATT CTTTAATGAA
    GATGGAGTTA TCAGACCTTA CAGATTGAGG GATGGTACTG GAAATCAAAT GTTACAGGCA
    TCAAAAAGTT TGATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50055
    Clone ID Related Accession (Same CDS sequence) XM_010807910.2 , XM_015464541.1
    Accession Version XM_015464541.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product vacuolar protein sorting-associated protein 13A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAAAAGA AAGCCAAAAA GGCCATTGTT GAGTCAGGTA CTGAAGAAGA AAATTACAGA 
    GTACCAGAAT ACAAAACTGT CATCACTTTC TATTCGAAAG ACCAGCTAAA CATTACATTA
    TCTAAATGTG GCCTTATAAT GTTAAATAAT TTAGCTGAGG CATTTACAGA AGCTGCCACT
    GGATCTTCAG CTACCTTCAT AAGGGATCTG GCACCTTTTA TGATTTTAAA TTCTCTTGGA
    CTTAGTATTT CTGTTTCACC AAGTGAATCT TTTAGTGTAC TCAACGTACC TATGGCAAAA
    TCATATGTAT TGAAAAATGA GGAGAGTTTA AGTATGGATT ATATGCGAAC TAAAGACAAT
    GATCATTTCA ATGCAATGAC CAGCCTAAGC AGCAAACACT TCTTCATTCT TCTTACCCCT
    GCTAACCACT CTACTGCTGA TAAAATCCCT TTAACAAAAG TGGGACGACG ACTGTACACT
    GTAAGACACA GAGAGTCTGG AGTTGAAAGA TCAATTGTTT GTCAGATTGA TGCAGTGGAA
    GGAAGTAAGA AGGTAACAGT TCGCTCTCCA GTGCAGATTA GAAATCATTT TTCAATACCA
    CTTTCTGTTT ATGGAGGAGA CACGTTATTG GGAACGGCCT CACCTGGAAG TGAATTTAAC
    ATACCATTAG CATCTTACCG ATCACTACTT TCTCTGAAGC CAGAAGATGC AGACTATCAG
    ATGTGTGAAG GAATTGACTT TGAAGAAATT ATAAAACAAG ATGGTACTTC TTTCAAGAAA
    AAATGTAGAC CTATAAACCC TTCCAGGAAG CCATTTATCA TTAATATAGT CCCAGAAAAA
    GATAATTTGA CGTATATGTC AGTGTATTCA GAAGACCGTT GGGACTTACC ATATATAATA
    CATTTGTGGC CACCTATTCT ACTCCGAAAT CTTCTTCCTT ACAAAATTGC TTATTACATA
    GAGGGGATTG AACATAGAGT TTTTACTCTC CATGAAGGCC GTTCAGCCCA GCTTTGTACT
    GTACAGTTGG ATAAAGCCAG GCTACATTTA AAATTACTTG ACTATCTTAA TCATGATTGG
    AAAAGTGAAT ATCATATAAA ACCTAATCAA CAAGATATTA GTTTCATCAG TTTTACTTGT
    GTTACAGAAA TAGAAAAGAC TGATTTAGAT ATTGCTGTCC ATGTGACTTA TAATACTGGC
    CAGACTATTA TGGCATTTCA TAGTCCTTAT TGGATGGTTA ATAAAACTGG TCGAATGTTG
    CAGTATAAAG CAGATGGAAT TCATCGAAAG CATCCACCTA ATTATAAAAA GCCAGTTCTC
    TTTTCATTTC AGCCAAAGCA CTTTTTCAAT AACAATAAGG TTCAGCTTAT GGTAACTGAT
    AGTGAATTGT CAGATCAATT TTCAATTGAT ACAGTTGGTA GTCATGGAGC GGTTAAATGT
    AAAGGCCCAA AAATGGAATA TCAAGTTGGT GTCACTATAG ACCTTAGCAG TTTTAACATT
    ACCAGAATTG TCACATTTAC CCCCTTTTTT TTGATTAAAA ACAAAAGCAA ATATCATATA
    TCAGTGGCTG AAGAGGGAAA TGATAAATGG CTCGCTCTTG ATTTGGAGCA GTGTGTCCCC
    TTTTGGCCTG AGGATGCTTC TAATAAACTT CTTATTCGAG TTGAAAGAAG TGAAAGCCCT
    CCCCATAAGA TACATTTTAA TAAGGAAGAA AGTTGTATTT TACTGCGTCT AGACAATGAG
    CTTGGAGGTA TTATAGCAGA AGTTAATTTG GCTGAGCATT CTACAGTTAT TACATTTTCA
    GATTATCGTG ATGGAGCCGC TACATTTCTG TTAATAAATC ACACCAAGAA TGACATTGTT
    CAGTATAAGC AAAGTTCCCT CAGTGAAATA GAAGATTCCC TCCCTCCTGG AAAAGCAGTA
    TTTTATACAT GGGCTGATCC AGTGGGCTCT AGAAAGCTGC AGTGGAAATG TGGAAAAAGC
    CACGGTGAAG TAACACAAAA GGATGATATG ATGACACCTA TAAATTTGGG GAAAACGACC
    ATTTATTTAG TTTCATTTTT TGAAGGCTTA CAACGCATAA TTTTATTCAC TGAAGATCCA
    AGAGTATTTA AAGTGACATA TGAAAGTGAG AAAGCAGAGT TAGCAGAGCA AGAAATTGTG
    TTGGCGTTAC AAGATGTTGG GATTTCTCTT GTCAACAATT ATACAAAGCA AGAAGTAGCC
    TATATTGGCA TTACAAGTTC TGATGTGGTT TGGGAGACAA AGCCCAGGAA GAAGGCAAGA
    TGGAAGCCAA TGAGTGTAAA GCACACTGAG AAGTTAGAGA AGGAATTTAA GGAATATGTT
    GAATCTTCAC CGTCAGAAGA TAAGGTTATT GAGTTGGACA CCAACATTCT GGTTCGCTTC
    ACACCTGGTG CTTCTAACAT GAAAATTCTG CAGCCACATG TAATAGCTAT AAGAAGAAAT
    TATCTTCCAG CATTAAAAGT AGAATATAGC ACATCTGCAC ATCAGTCATC GTTTAGAATT
    CAAATTTATA GAATACAGAT CCAAAACCAA ATACATGGTG CTATATTTCC ATTCGTGTTT
    TATCCCATTA AACCGCCCAA GTCCGTCACC TTGGATTCAG CACCGAAACC CTTTACTGAT
    GTCAGTATTG TCATGAGATC TGCAGGACAT TCGCAGATAT CACGTATTAA GTATTTCAAA
    GTGTTGATCC AAGAAATGGA TCTCAGGTTA GATCTTGGAT TTGTCTATGC TGTATCAGAA
    CTTATGACAC CAGCTGAAGT CACTGAAAAA ACAGAGGTTG AGCTTTTTCA TAAAGACATA
    GAAGCTTTCC AAGAAGAATA TAAAATAGTC TCATTAGTAG ATTCATCACA AGTCAGTCTC
    TATGAATACT TTCATATATC TCCTATCAAG TTGCATTTAA GTGTTTCACT GAGTTCAGGT
    GGTGAAGAAC CGAAAGATTC AGAACAGCAT GAAGGACTGA TGGCAATTCG TTCTTTACAT
    CTTTTGCTGA AGAGTATTGG TGCCACTCTC ACAGATGTAC AAGATGTAGT TTTTAAGCTA
    GCATTTTTTG AACTCAATTA TCAGTTCCAT ACAACAGCTG AACTACAGTC TGAAGTAATA
    AGACACTATT CAAAGCAGGC CATTAAACAG ATGTACGTAC TCATTCTTGG ACTTGATGTT
    TTGGGAAATC CCTTTGGCTT GATAAGAGAA TTTTCTGAAG GTGTAGAAGC ATTTTTTTAT
    GAACCTTACC AGGGAGCCAT CCAGGGTCCT GAAGAGTTTG TGGAAGGAAT GGCACTAGGA
    CTGAAAGCAC TAGTTGGTGG AGCTGTTGGT GGACTAGCTG GTGCTGCCTC CAAAATCACC
    AGTGCTATGG CTAAAGGGGT GGCAGCTATG ACTATGGATG AAGACTACCA ACAGAAGAGA
    AGAGAAGCCA TGAATAAGCA ACCAGCTGGT CTTAGGGAAG GCATCACTCG TGGAGGAAAA
    GGCCTAGTTT CTGGTTTTGT AAGTGGCATA ACAGGAATTG TTACAAAACC AATCAAAGGA
    GCTCAGAAAG AAGGAGCAAC TGGTTTCTTT AAAGGTGTTG GGAAAGGTTT AGTTGGAGCA
    GTGACAAGGC CAACTGGAGG CATCATAGAC ATGGCTAGCA GTACATTTCA GGGAATAAAA
    AGAGCTACAG AGACCTCTGA TGAAGTGGAG AGTCTGCGAC CCCCTAGATT CTTTAATGAA
    GATGGAGTTA TCAGACCTTA CAGATTGAGG GATGGTACTG GAAATCAAAT GTTACAGGCA
    TCAAAAAGTT TGATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320027.1
    CDS21..3818
    Translation

    Target ORF information:

    RefSeq Version XM_015464541.1
    Organism Bos taurus(cattle)
    Definition Bos taurus vacuolar protein sorting 13 homolog A (S. cerevisiae) (VPS13A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464541.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAAAAGA AAGCCAAAAA GGCCATTGTT GAGTCAGGTA CTGAAGAAGA AAATTACAGA 
    GTACCAGAAT ACAAAACTGT CATCACTTTC TATTCGAAAG ACCAGCTAAA CATTACATTA
    TCTAAATGTG GCCTTATAAT GTTAAATAAT TTAGCTGAGG CATTTACAGA AGCTGCCACT
    GGATCTTCAG CTACCTTCAT AAGGGATCTG GCACCTTTTA TGATTTTAAA TTCTCTTGGA
    CTTAGTATTT CTGTTTCACC AAGTGAATCT TTTAGTGTAC TCAACGTACC TATGGCAAAA
    TCATATGTAT TGAAAAATGA GGAGAGTTTA AGTATGGATT ATATGCGAAC TAAAGACAAT
    GATCATTTCA ATGCAATGAC CAGCCTAAGC AGCAAACACT TCTTCATTCT TCTTACCCCT
    GCTAACCACT CTACTGCTGA TAAAATCCCT TTAACAAAAG TGGGACGACG ACTGTACACT
    GTAAGACACA GAGAGTCTGG AGTTGAAAGA TCAATTGTTT GTCAGATTGA TGCAGTGGAA
    GGAAGTAAGA AGGTAACAGT TCGCTCTCCA GTGCAGATTA GAAATCATTT TTCAATACCA
    CTTTCTGTTT ATGGAGGAGA CACGTTATTG GGAACGGCCT CACCTGGAAG TGAATTTAAC
    ATACCATTAG CATCTTACCG ATCACTACTT TCTCTGAAGC CAGAAGATGC AGACTATCAG
    ATGTGTGAAG GAATTGACTT TGAAGAAATT ATAAAACAAG ATGGTACTTC TTTCAAGAAA
    AAATGTAGAC CTATAAACCC TTCCAGGAAG CCATTTATCA TTAATATAGT CCCAGAAAAA
    GATAATTTGA CGTATATGTC AGTGTATTCA GAAGACCGTT GGGACTTACC ATATATAATA
    CATTTGTGGC CACCTATTCT ACTCCGAAAT CTTCTTCCTT ACAAAATTGC TTATTACATA
    GAGGGGATTG AACATAGAGT TTTTACTCTC CATGAAGGCC GTTCAGCCCA GCTTTGTACT
    GTACAGTTGG ATAAAGCCAG GCTACATTTA AAATTACTTG ACTATCTTAA TCATGATTGG
    AAAAGTGAAT ATCATATAAA ACCTAATCAA CAAGATATTA GTTTCATCAG TTTTACTTGT
    GTTACAGAAA TAGAAAAGAC TGATTTAGAT ATTGCTGTCC ATGTGACTTA TAATACTGGC
    CAGACTATTA TGGCATTTCA TAGTCCTTAT TGGATGGTTA ATAAAACTGG TCGAATGTTG
    CAGTATAAAG CAGATGGAAT TCATCGAAAG CATCCACCTA ATTATAAAAA GCCAGTTCTC
    TTTTCATTTC AGCCAAAGCA CTTTTTCAAT AACAATAAGG TTCAGCTTAT GGTAACTGAT
    AGTGAATTGT CAGATCAATT TTCAATTGAT ACAGTTGGTA GTCATGGAGC GGTTAAATGT
    AAAGGCCCAA AAATGGAATA TCAAGTTGGT GTCACTATAG ACCTTAGCAG TTTTAACATT
    ACCAGAATTG TCACATTTAC CCCCTTTTTT TTGATTAAAA ACAAAAGCAA ATATCATATA
    TCAGTGGCTG AAGAGGGAAA TGATAAATGG CTCGCTCTTG ATTTGGAGCA GTGTGTCCCC
    TTTTGGCCTG AGGATGCTTC TAATAAACTT CTTATTCGAG TTGAAAGAAG TGAAAGCCCT
    CCCCATAAGA TACATTTTAA TAAGGAAGAA AGTTGTATTT TACTGCGTCT AGACAATGAG
    CTTGGAGGTA TTATAGCAGA AGTTAATTTG GCTGAGCATT CTACAGTTAT TACATTTTCA
    GATTATCGTG ATGGAGCCGC TACATTTCTG TTAATAAATC ACACCAAGAA TGACATTGTT
    CAGTATAAGC AAAGTTCCCT CAGTGAAATA GAAGATTCCC TCCCTCCTGG AAAAGCAGTA
    TTTTATACAT GGGCTGATCC AGTGGGCTCT AGAAAGCTGC AGTGGAAATG TGGAAAAAGC
    CACGGTGAAG TAACACAAAA GGATGATATG ATGACACCTA TAAATTTGGG GAAAACGACC
    ATTTATTTAG TTTCATTTTT TGAAGGCTTA CAACGCATAA TTTTATTCAC TGAAGATCCA
    AGAGTATTTA AAGTGACATA TGAAAGTGAG AAAGCAGAGT TAGCAGAGCA AGAAATTGTG
    TTGGCGTTAC AAGATGTTGG GATTTCTCTT GTCAACAATT ATACAAAGCA AGAAGTAGCC
    TATATTGGCA TTACAAGTTC TGATGTGGTT TGGGAGACAA AGCCCAGGAA GAAGGCAAGA
    TGGAAGCCAA TGAGTGTAAA GCACACTGAG AAGTTAGAGA AGGAATTTAA GGAATATGTT
    GAATCTTCAC CGTCAGAAGA TAAGGTTATT GAGTTGGACA CCAACATTCT GGTTCGCTTC
    ACACCTGGTG CTTCTAACAT GAAAATTCTG CAGCCACATG TAATAGCTAT AAGAAGAAAT
    TATCTTCCAG CATTAAAAGT AGAATATAGC ACATCTGCAC ATCAGTCATC GTTTAGAATT
    CAAATTTATA GAATACAGAT CCAAAACCAA ATACATGGTG CTATATTTCC ATTCGTGTTT
    TATCCCATTA AACCGCCCAA GTCCGTCACC TTGGATTCAG CACCGAAACC CTTTACTGAT
    GTCAGTATTG TCATGAGATC TGCAGGACAT TCGCAGATAT CACGTATTAA GTATTTCAAA
    GTGTTGATCC AAGAAATGGA TCTCAGGTTA GATCTTGGAT TTGTCTATGC TGTATCAGAA
    CTTATGACAC CAGCTGAAGT CACTGAAAAA ACAGAGGTTG AGCTTTTTCA TAAAGACATA
    GAAGCTTTCC AAGAAGAATA TAAAATAGTC TCATTAGTAG ATTCATCACA AGTCAGTCTC
    TATGAATACT TTCATATATC TCCTATCAAG TTGCATTTAA GTGTTTCACT GAGTTCAGGT
    GGTGAAGAAC CGAAAGATTC AGAACAGCAT GAAGGACTGA TGGCAATTCG TTCTTTACAT
    CTTTTGCTGA AGAGTATTGG TGCCACTCTC ACAGATGTAC AAGATGTAGT TTTTAAGCTA
    GCATTTTTTG AACTCAATTA TCAGTTCCAT ACAACAGCTG AACTACAGTC TGAAGTAATA
    AGACACTATT CAAAGCAGGC CATTAAACAG ATGTACGTAC TCATTCTTGG ACTTGATGTT
    TTGGGAAATC CCTTTGGCTT GATAAGAGAA TTTTCTGAAG GTGTAGAAGC ATTTTTTTAT
    GAACCTTACC AGGGAGCCAT CCAGGGTCCT GAAGAGTTTG TGGAAGGAAT GGCACTAGGA
    CTGAAAGCAC TAGTTGGTGG AGCTGTTGGT GGACTAGCTG GTGCTGCCTC CAAAATCACC
    AGTGCTATGG CTAAAGGGGT GGCAGCTATG ACTATGGATG AAGACTACCA ACAGAAGAGA
    AGAGAAGCCA TGAATAAGCA ACCAGCTGGT CTTAGGGAAG GCATCACTCG TGGAGGAAAA
    GGCCTAGTTT CTGGTTTTGT AAGTGGCATA ACAGGAATTG TTACAAAACC AATCAAAGGA
    GCTCAGAAAG AAGGAGCAAC TGGTTTCTTT AAAGGTGTTG GGAAAGGTTT AGTTGGAGCA
    GTGACAAGGC CAACTGGAGG CATCATAGAC ATGGCTAGCA GTACATTTCA GGGAATAAAA
    AGAGCTACAG AGACCTCTGA TGAAGTGGAG AGTCTGCGAC CCCCTAGATT CTTTAATGAA
    GATGGAGTTA TCAGACCTTA CAGATTGAGG GATGGTACTG GAAATCAAAT GTTACAGGCA
    TCAAAAAGTT TGATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237445
    Clone ID Related Accession (Same CDS sequence) XM_024996187.1 , XM_024996188.1
    Accession Version XM_024996187.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product vacuolar protein sorting-associated protein 13A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGGTGTTCG AGTCGGTGGT CGTGGACGTG TTGAACCGGT TCTTGGGGGA CTATGTGGTG 
    AACCTGGACA CGTCCCAGCT CAGTCTGGGC ATCTGGGCAG GAGCTGTGGC CCTGAAGAAT
    CTTGAAATTA AAGAAAATGC CCTGAGTCAA CTGGACGTAC CATTTAAAAT TAAAGTTGGC
    CATATAGGTA CTCTTAATCT TATAATTCCA TGGAAAAACC TTTATACCCA ACCAGTTGAA
    GCTGTATTGG AAGGCGTTTA TTTACTCATA GTGCCTTCTT CTAGAATAAA ATATGATCCT
    ATAAAAGAAG AAAAACAACT CTTTGAAGCA AAGCAACAGG AATTGAAAAG AATAGAAGAA
    GCAAAACGAA AAGTAGCTGA TCAAGAAAAA CAGCTTGTGG AGAAACAGGA CACTTTCACA
    GAAAAATTAA TTACCCAGAT CATAAAAAAT CTTCAAGTGA AAATTTCCAA CATCCATATT
    CGCTATGAAG ATGATGTCAC AAATCGAGAA AACCCTCTGT CATTTGGTAT TTCCCTTCAG
    AATCTCAGCA TGCAGACAAC TGATCAATAC TGGATTCCAT GTTTACATGA TGAAACTGAG
    AAATTATTTC GTAAGTTAAT CCGATTGGAT AACCTTTTTG CCTATTGGAA TGTGAAGACT
    CAGTTGTTTT ATCTTAGTAA TTATGATGAA TCAGTGAGCA GCCTGAGGAA TGGCATTGTT
    GATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTATTTC GCCCCATATC TGCCAATGCC
    AAACTTCAGA TGAATCGCAG ATCGGATTTT GACTTTTCTG CCCCCAAAAT AGACCTGGAT
    GTTGAGTTAC ATGACATAAC AATTCAACTT AATAAACCAC AGTATTTCAG TTTTATGGAA
    CTTCTTGAAT CGGTTGATAT GATGACGCAA AATGTACCAT ATAGGAAATT CAAACCTGAT
    GTACCTCTTC GTTACCATGC CAAAGAATGG TGGGCTTATG CTATACATGG TATCCTTGAA
    GTAAACATTC GCCCCCACTT ACGGATGTGG TCCTGGAAGC ACATTAGAGA ACATAGGCAA
    AAAATGAAGC AATATAAAGA ACTGTATAAA AAAAAGCTAA CAAGTAAGAA GCCATCTGGT
    GAACTTCTCA ACTCTTTGGA GGATTTGGAA AAGACTTTGG ATGTGTTTAA TATAACTGTA
    GCAAGACAAC AGGCGGAAGT TGAGGTAAAG AAAGCTGGAT ACAGGATTTT CAGAGAAGGA
    GCAGAAGAGT CCGAGGGCAA TAAAGGATGG TTTAGCTGGA TGTGGAGTTG GTCAGAATCG
    CATAGTAAAC AACAACTAGA TATTAAACCT GGAAGTCTTG AAGAAATATT GACACCTAAA
    GAAAAAAGTT TGCTGTATGA AGCAATTGGC TATAGTGAAA CAGCAGTTGA TCCAACCTTG
    CCAAAAACAT TTGAAGCAAT GAAGTTCTTT GTTCACTTGA AAAGTATGTC TATTATTCTA
    AGAGAACAAC AACAAAAACC TGAACTGCTA GATATTGTAG TGGAAGAATT TAGTACTTTG
    ATTGTGCAAA GACCAGGAGC ACAAGCAGTT AAATTTGAAA CCAAAATAGA TTCATTTCAT
    GTTACTGGCT TACCAGATAA CTCAACAAAA CCCCGCCTCC TGTCTTCATT AGATGATGCT
    GCATCACTCT TGAAAATTAT GTTTGAGATA AATCCTTTAG ATGAAACTGT TGCTCAGAGG
    TGTATCATAG AAGCTGAACC TTTAGAAATG TTATATGATG CAAGAACAGT GAATAGTATA
    GTAGAATTCT TCAGACCTCC AAAAGAGGTA AATCTAGCAC AGCTGACTTC AGTAACTTTG
    ACAAAACTTG AAGAATTTCG TGATAAGACA GCAACAGGTC TGGTATATAT TATTGAAACA
    CAGAAAGTTC TTGATCTCAA AATTAACTTG AATGCTTCAT ATATCATTAT CCCACAAGAT
    GGTATCTTTA GCCCTACGTC AAATCTCCTT CTTTTGGATC TTGGACATCT AAAGGTGACA
    AGTAAAATTC GTTCAGAATT ACCAGATTTA AAACAAGGTG GAGCCAATCG TGAAGAGATA
    ATGCATAGAG CTTATGATTC CTTTGATATT CAACTTACAA GCATACAGCT GCTTTTCAGC
    AGAGTTGGTG ATAATTGGAA AGAAGCACGA AAGCTCAATG TGTCTGCACA GCATATCTTG
    GTACCTATGC ACTTCAATGT GGAGCTCTCT AAGGCCATGG TTTTCATGGA TATAAGTATG
    CCGAAATTCA AGATTTATGG AAACTTACCA TTAATTTCTT TACGAATCTC AGATAAAAAA
    CTGCAAGGGA TTTTGGAACT GATTGAAAGC ATTCCAAAAC CCTCACCTGC AACTGAAGTA
    AATGTGCCTG TCAAATCATC TCAGGTTCAA AGATCCACTT CTTTGGGAAC ATCACAGATG
    TCACAGAAGA AATTTCCTCT TTTGGAAATT CCATGTGTTG AAGATGATTC AGATGAGGAG
    TTTTTTGATG CACCATGTAG TCCGTTGGAA GAGCCTCTTC AATCACCATC TAGAATTAAA
    AGTCCTCAAC AGAAAAATCT TCAGAAACCA GATTGTTTTA AAAATATGAT TGAGTATAAA
    ATAAGATTTG AAGTGCCAGA GGTTTTGATT CAGTTCTGTC ACCTTGTTGG AGACTGTGAA
    CTACCCGTGG TAGAAATTAA TGTCTTAGGA TTGGGCACAG ATATTGAGCT TAGAACATTT
    GATTTGAAAG CAAATGCCTT TTTGAAAGAG GTCTGCTTAA AATGTCCAGA TTATTTTGAT
    GAAAACAAGA AACCAGTTTA TTTGATTACA ACACTGGATA ACACAATGGA AGATCTCTTA
    ACACTGGAAT ATGTGAAGGC TGAAAATAAT TTACCTGCAA CAATATTGAA GAATGCTTAT
    AACAGTGTTG TGCAACTGAT AAAGGTGAAT TTTTCCTCTT TGGATATTCA TTTACATACT
    GAAGCACTTT TGAATACAAT AAATTATTTT AATAATATCC TTCCATATTT GGAGGAAAAA
    CCAGCCCCAG TGCATGTTGT GGAGACTGAA GACAAAGAGG AGGTTGTTAA AAAAATACCT
    TTAAAATCAT CCAAGAATGA AGATACTATT ACCTTGCAGA TTTTAGCAGA ATTATCATGT
    TTACAGATCT TTATTCAAGA TCAGAAACGT AACATTTCCG AAATTAAGAT AGAAGGGCTT
    GATTGTGAAA TCATTATGAG GCCATCAGTG ACTGAAACAA ATGCAAAGCT AAGGAATATA
    ATTGTCCTGG ATTCTGATAT AACAGCTGTA TACAAAAAGG CTGTTTATAT TACCGGAAAA
    GAAGTTTTCA GCTTCAAAAT GGTTTCTTAT ATGGGTGCAA CTGCTGGTTC TGCATACACA
    GATATGAATG TAGTTGATAT GCAAGTTAAT TTAACTGTTG GTTGCATTGA AGTAGTTCTT
    GTCAAAAAAT TTCTGTATTC TATATTGGCT TTTATAGATA ATTTTCAAGC AGGTAAACAA
    GCCTTAGCGG AGGCAACTGT TCAGGCAGCA GGAATGGCTG CTACTGGTGT AAAAGAACTG
    GCACAGAGGA GTTCTAGAAT GGCATTGGAT GTTCACATCA AAGCCCCTGT TGTGGTTATC
    CCACAGTCTC CAGTTTCTGA AAATATTTTT GTTGCTGATT TTGGACTAAT TACAATGAAA
    AATACATTCC ATGTAATAAC AAAGAGTCCG ACCAACCCCC CACCCATTAT TGATTTGATA
    ACGATAAAGC TAAGTGAAAT GCGACTATAC AGGACTCAAT ATATTAATGA TGCATACCAG
    GAAGTACTGG ATCTACTATT GCCATTAAAT CTTGAGGTGA TGGTTGAGCG AAATTTATCG
    TGGGAGTGGT TCCAGGAAGT TCCTTGTTTC AATCTAAATG CTCAGCTAAA GCCCATGGAG
    TTCATTCTTA GTCAAGAAGA TATAACGACC ATTTTTAAAA CATTATATGG CAACATATGG
    TATGAAGGTG ATAGTGCTTC ACATGAGGTA ACAAAGGATC AGTCCAGTGT TACTAGTGGT
    GCTACTACTG CGTCACACCA TGCGGCAGGA ACAACTGTAG TGACAGCTGC TGTGGTAGAA
    GTACATTCAC ATGCTTCTCA AGTTAAGACA ACACTAAATA TGAGTTTCAG AACCGATTAT
    CTCACCATGG TACTGTATAG TCCAGGTCCT AAACAGACTT CCTTTACAGA TATTCGTGAT
    CCTTCTCTGA AACTTGCTGA ATTTAAGTTG GAGAATATTA TAAGCACTTT AAAAATGTAT
    ACAGATGACT CAATATTTTC TTCCTTCTCA TTAAAAAACT GTATTTTAGA TGACAAAAGG
    CCTCATATCA AGAAGGCAAC TCCTAGAATG ATAGGACTGA CAGTTGGGTT TGACAAAAAG
    GACATGATGG ATGTAAGGTA TAGGACGATC CGAGATGGTT GGGTGGCTGA TATAGTCCTT
    CAAGAAATGT ATATTTGTGC AAGTGTGGAA TTTCTGCTGA CAGTTGCAAA TGTCTTTCTT
    GAGGCCTACA CTACAGGCAC TGCTGTAGAA ACCAATGTTC AAACATGGTC TGCCAAAGAA
    GAAGTACCTA AACAGCAATC AGAAAAGTGG GAAATGAACA TCACTATTAA AAATCCTGAA
    ATTGTGTTTG TCGCTGATAT GACAAGAAAT GATGCTCCTG CCTTAGTCAT TACAACACAG
    TGTGAAATAT ATTGGCTAGG TGACTTTGAA AATGATACAA TGACTGCTGC CATTAAAGAT
    CTCCAAGTGA GAGCATGCCC ATTTCTTCCA GTCAAGAGAA AAGGAAAAAT CACTACTGTT
    TTGCAACCCT GTGATTTGTT CTATCAAGCT ACTCAGATAA GTACAGATCC ACAAGTACTT
    GAATTATCAG TAAAATCCCT GACACTGAAG GTTTCACCAG TTATCATAAA TACTGTGATT
    ACCATAACTT CAGCACTTTA TACTGCTAAG GAAACCATCC CAAAAGAAAC TGCTTCTTCT
    ACAGCACATT TATGGGAAAA GAAGGATACA AAAGATTTAA ATATGTGGTT TCTTGAAGAA
    CCAAATGAAG CTGTAAAAAG AGCTCCCATG CCTGAATTGG TACCCAAAGG AGAGATAATA
    AAAATGAACA TTGATTCTAT TTTTCTGGTT CTTGAGGCTG GAATTGGTCA TAGAACAGTA
    CCTATGCTAT TGGCAAAATC ACGTTTCTCA GGGGAAGGCA AAAACTGGAG TTCCTTAATA
    AATCTCCACT GTCAGCTTGA GCTAGAAGTA CATTATTACA ATGAAATGTT TGGTGTGTGG
    GAGCCTTTGC TTGAACCCTT AGAAATTGAT CAGACTGAGG ATTTTAGACC ATGGAATCTT
    GGAATCAAGA TGAAAAAGAA AGCCAAAAAG GCCATTGTTG AGTCAGGTAC TGAAGAAGAA
    AATTACAGAG TACCAGAATA CAAAACTGTC ATCACTTTCT ATTCGAAAGA CCAGCTAAAC
    ATTACATTAT CTAAATGTGG CCTTATAATG TTAAATAATT TAGCTGAGGC ATTTACAGAA
    GCTGCCACTG GATCTTCAGC TACCTTCATA AGGGATCTGG CACCTTTTAT GATTTTAAAT
    TCTCTTGGAC TTAGTATTTC TGTTTCACCA AGTGAATCTT TTAGTGTACT CAACGTACCT
    ATGGCAAAAT CATATGTATT GAAAAATGAG GAGAGTTTAA GTATGGATTA TATGCGAACT
    AAAGACAATG ATCATTTCAA TGCAATGACC AGCCTAAGCA GCAAACACTT CTTCATTCTT
    CTTACCCCTG CTAACCACTC TACTGCTGAT AAAATCCCTT TAACAAAAGT GGGACGACGA
    CTGTACACTG TAAGACACAG AGAGTCTGGA GTTGAAAGAT CAATTGTTTG TCAGATTGAT
    GCAGTGGAAG GAAGTAAGAA GGTAACAGTT CGCTCTCCAG TGCAGATTAG AAATCATTTT
    TCAATACCAC TTTCTGTTTA TGGAGGAGAC ACGTTATTGG GAACGGCCTC ACCTGGAAGT
    GAATTTAACA TACCATTAGC ATCTTACCGA TCACTACTTT CTCTGAAGCC AGAAGATGCA
    GACTATCAGA TGTGTGAAGG AATTGACTTT GAAGAAATTA TAAAACAAGA TGGTACTTCT
    TTCAAGAAAA AATGTAGACC TATAAACCCT TCCAGGAAGC CATTTATCAT TAATATAGTC
    CCAGAAAAAG ATAATTTGAC GTATATGTCA GTGTATTCAG AAGACCGTTG GGACTTACCA
    TATATAATAC ATTTGTGGCC ACCTATTCTA CTCCGAAATC TTCTTCCTTA CAAAATTGCT
    TATTACATAG AGGGGATTGA ACATAGAGTT TTTACTCTCC ATGAAGGCCG TTCAGCCCAG
    CTTTGTACTG TACAGTTGGA TAAAGCCAGG CTACATTTAA AATTACTTGA CTATCTTAAT
    CATGATTGGA AAAGTGAATA TCATATAAAA CCTAATCAAC AAGATATTAG TTTCATCAGT
    TTTACTTGTG TTACAGAAAT AGAAAAGACT GATTTAGATA TTGCTGTCCA TGTGACTTAT
    AATACTGGCC AGACTATTAT GGCATTTCAT AGTCCTTATT GGATGGTTAA TAAAACTGGT
    CGAATGTTGC AGTATAAAGC AGATGGAATT CATCGAAAGC ATCCACCTAA TTATAAAAAG
    CCAGTTCTCT TTTCATTTCA GCCAAAGCAC TTTTTCAATA ACAATAAGGT TCAGCTTATG
    GTAACTGATA GTGAATTGTC AGATCAATTT TCAATTGATA CAGTTGGTAG TCATGGAGCG
    GTTAAATGTA AAGGCCCAAA AATGGAATAT CAAGTTGGTG TCACTATAGA CCTTAGCAGT
    TTTAACATTA CCAGAATTGT CACATTTACC CCCTTTTTTT TGATTAAAAA CAAAAGCAAA
    TATCATATAT CAGTGGCTGA AGAGGGAAAT GATAAATGGC TCGCTCTTGA TTTGGAGCAG
    TGTGTCCCCT TTTGGCCTGA GGATGCTTCT AATAAACTTC TTATTCGAGT TGAAAGAAGT
    GAAAGCCCTC CCCATAAGAT ACATTTTAAT AAGGAAGAAA GTTGTATTTT ACTGCGTCTA
    GACAATGAGC TTGGAGGTAT TATAGCAGAA GTTAATTTGG CTGAGCATTC TACAGTTATT
    ACATTTTCAG ATTATCGTGA TGGAGCCGCT ACATTTCTGT TAATAAATCA CACCAAGAAT
    GACATTGTTC AGTATAAGCA AAGTTCCCTC AGTGAAATAG AAGATTCCCT CCCTCCTGGA
    AAAGCAGTAT TTTATACATG GGCTGATCCA GTGGGCTCTA GAAAGCTGCA GTGGAAATGT
    GGAAAAAGCC ACGGTGAAGT AACACAAAAG GATGATATGA TGACACCTAT AAATTTGGGG
    AAAACGACCA TTTATTTAGT TTCATTTTTT GAAGGCTTAC AACGCATAAT TTTATTCACT
    GAAGATCCAA GAGTATTTAA AGTGACATAT GAAAGTGAGA AAGCAGAGTT AGCAGAGCAA
    GAAATTGTGT TGGCGTTACA AGATGTTGGG ATTTCTCTTG TCAACAATTA TACAAAGCAA
    GAAGTAGCCT ATATTGGCAT TACAAGTTCT GATGTGGTTT GGGAGACAAA GCCCAGGAAG
    AAGGCAAGAT GGAAGCCAAT GAGTGTAAAG CACACTGAGA AGTTAGAGAA GGAATTTAAG
    GAATATGTTG AATCTTCACC GTCAGAAGAT AAGGTTATTG AGTTGGACAC CAACATTCTG
    GTTCGCTTCA CACCTGGTGC TTCTAACATG AAAATTCTGC AGCCACATGT AATAGCTATA
    AGAAGAAATT ATCTTCCAGC ATTAAAAGTA GAATATAGCA CATCTGCACA TCAGTCATCG
    TTTAGAATTC AAATTTATAG AATACAGATC CAAAACCAAA TACATGGTGC TATATTTCCA
    TTCGTGTTTT ATCCCATTAA ACCGCCCAAG TCCGTCACCT TGGATTCAGC ACCGAAACCC
    TTTACTGATG TCAGTATTGT CATGAGATCT GCAGGACATT CGCAGATATC ACGTATTAAG
    TATTTCAAAG TGTTGATCCA AGAAATGGAT CTCAGGTTAG ATCTTGGATT TGTCTATGCT
    GTATCAGAAC TTATGACACC AGCTGAAGTC ACTGAAAAAA CAGAGGTTGA GCTTTTTCAT
    AAAGACATAG AAGCTTTCCA AGAAGAATAT AAAATAGTCT CATTAGTAGA TTCATCACAA
    GTCAGTCTCT ATGAATACTT TCATATATCT CCTATCAAGT TGCATTTAAG TGTTTCACTG
    AGTTCAGGTG GTGAAGAACC GAAAGATTCA GAACAGCATG AAGGACTGAT GGCAATTCGT
    TCTTTACATC TTTTGCTGAA GAGTATTGGT GCCACTCTCA CAGATGTACA AGATGTAGTT
    TTTAAGCTAG CATTTTTTGA ACTCAATTAT CAGTTCCATA CAACAGCTGA ACTACAGTCT
    GAAGTAATAA GACACTATTC AAAGCAGGCC ATTAAACAGA TGTACGTACT CATTCTTGGA
    CTTGATGTTT TGGGAAATCC CTTTGGCTTG ATAAGAGAAT TTTCTGAAGG TGTAGAAGCA
    TTTTTTTATG AACCTTACCA GGGAGCCATC CAGGGTCCTG AAGAGTTTGT GGAAGGAATG
    GCACTAGGAC TGAAAGCACT AGTTGGTGGA GCTGTTGGTG GACTAGCTGG TGCTGCCTCC
    AAAATCACCA GTGCTATGGC TAAAGGGGTG GCAGCTATGA CTATGGATGA AGACTACCAA
    CAGAAGAGAA GAGAAGCCAT GAATAAGCAA CCAGCTGGTC TTAGGGAAGG CATCACTCGT
    GGAGGAAAAG GCCTAGTTTC TGGTTTTGTA AGTGGCATAA CAGGAATTGT TACAAAACCA
    ATCAAAGGAG CTCAGAAAGA AGGAGCAACT GGTTTCTTTA AAGGTGTTGG GAAAGGTTTA
    GTTGGAGCAG TGACAAGGCC AACTGGAGGC ATCATAGACA TGGCTAGCAG TACATTTCAG
    GGAATAAAAA GAGCTACAGA GACCTCTGAT GAAGTGGAGA GTCTGCGACC CCCTAGATTC
    TTTAATGAAG ATGGAGTTAT CAGACCTTAC AGATTGAGGG ATGGTACTGG AAATCAAATG
    TTACAGGTCA TGGAAAACGG AAGATTTGCA AAATACAAAT ATTTTACCCA TGTCATGATC
    AATAAGACAG ACATGTTAAT GATTACCAGA AGTGGTGTGT TGTTTGTAAC AAAAGGAACA
    TTTGGACAGC TTACTTGTGA GTGGCAGTAT AGTTTTGATG AGTTTACCAA AGAGCCATTC
    ATTGTTCATG AGAGAAGACT ACGCATCGAA GCAAAGGAAC GGGTGAAGTC TGTATTCCAT
    GCCAAAGAGT TTGGAAAAAT AGTTAACTTC AGAACCCCGG AGGATGCGAG GTGGATCCTC
    AGAAAGCTAG AAGAAGCAAG AGAACCTTCC CCGAACCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851955.1
    CDS255..9776
    Translation

    Target ORF information:

    RefSeq Version XM_024996187.1
    Organism Bos taurus(cattle)
    Definition Bos taurus vacuolar protein sorting 13 homolog A (VPS13A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996187.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGGTGTTCG AGTCGGTGGT CGTGGACGTG TTGAACCGGT TCTTGGGGGA CTATGTGGTG 
    AACCTGGACA CGTCCCAGCT CAGTCTGGGC ATCTGGGCAG GAGCTGTGGC CCTGAAGAAT
    CTTGAAATTA AAGAAAATGC CCTGAGTCAA CTGGACGTAC CATTTAAAAT TAAAGTTGGC
    CATATAGGTA CTCTTAATCT TATAATTCCA TGGAAAAACC TTTATACCCA ACCAGTTGAA
    GCTGTATTGG AAGGCGTTTA TTTACTCATA GTGCCTTCTT CTAGAATAAA ATATGATCCT
    ATAAAAGAAG AAAAACAACT CTTTGAAGCA AAGCAACAGG AATTGAAAAG AATAGAAGAA
    GCAAAACGAA AAGTAGCTGA TCAAGAAAAA CAGCTTGTGG AGAAACAGGA CACTTTCACA
    GAAAAATTAA TTACCCAGAT CATAAAAAAT CTTCAAGTGA AAATTTCCAA CATCCATATT
    CGCTATGAAG ATGATGTCAC AAATCGAGAA AACCCTCTGT CATTTGGTAT TTCCCTTCAG
    AATCTCAGCA TGCAGACAAC TGATCAATAC TGGATTCCAT GTTTACATGA TGAAACTGAG
    AAATTATTTC GTAAGTTAAT CCGATTGGAT AACCTTTTTG CCTATTGGAA TGTGAAGACT
    CAGTTGTTTT ATCTTAGTAA TTATGATGAA TCAGTGAGCA GCCTGAGGAA TGGCATTGTT
    GATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTATTTC GCCCCATATC TGCCAATGCC
    AAACTTCAGA TGAATCGCAG ATCGGATTTT GACTTTTCTG CCCCCAAAAT AGACCTGGAT
    GTTGAGTTAC ATGACATAAC AATTCAACTT AATAAACCAC AGTATTTCAG TTTTATGGAA
    CTTCTTGAAT CGGTTGATAT GATGACGCAA AATGTACCAT ATAGGAAATT CAAACCTGAT
    GTACCTCTTC GTTACCATGC CAAAGAATGG TGGGCTTATG CTATACATGG TATCCTTGAA
    GTAAACATTC GCCCCCACTT ACGGATGTGG TCCTGGAAGC ACATTAGAGA ACATAGGCAA
    AAAATGAAGC AATATAAAGA ACTGTATAAA AAAAAGCTAA CAAGTAAGAA GCCATCTGGT
    GAACTTCTCA ACTCTTTGGA GGATTTGGAA AAGACTTTGG ATGTGTTTAA TATAACTGTA
    GCAAGACAAC AGGCGGAAGT TGAGGTAAAG AAAGCTGGAT ACAGGATTTT CAGAGAAGGA
    GCAGAAGAGT CCGAGGGCAA TAAAGGATGG TTTAGCTGGA TGTGGAGTTG GTCAGAATCG
    CATAGTAAAC AACAACTAGA TATTAAACCT GGAAGTCTTG AAGAAATATT GACACCTAAA
    GAAAAAAGTT TGCTGTATGA AGCAATTGGC TATAGTGAAA CAGCAGTTGA TCCAACCTTG
    CCAAAAACAT TTGAAGCAAT GAAGTTCTTT GTTCACTTGA AAAGTATGTC TATTATTCTA
    AGAGAACAAC AACAAAAACC TGAACTGCTA GATATTGTAG TGGAAGAATT TAGTACTTTG
    ATTGTGCAAA GACCAGGAGC ACAAGCAGTT AAATTTGAAA CCAAAATAGA TTCATTTCAT
    GTTACTGGCT TACCAGATAA CTCAACAAAA CCCCGCCTCC TGTCTTCATT AGATGATGCT
    GCATCACTCT TGAAAATTAT GTTTGAGATA AATCCTTTAG ATGAAACTGT TGCTCAGAGG
    TGTATCATAG AAGCTGAACC TTTAGAAATG TTATATGATG CAAGAACAGT GAATAGTATA
    GTAGAATTCT TCAGACCTCC AAAAGAGGTA AATCTAGCAC AGCTGACTTC AGTAACTTTG
    ACAAAACTTG AAGAATTTCG TGATAAGACA GCAACAGGTC TGGTATATAT TATTGAAACA
    CAGAAAGTTC TTGATCTCAA AATTAACTTG AATGCTTCAT ATATCATTAT CCCACAAGAT
    GGTATCTTTA GCCCTACGTC AAATCTCCTT CTTTTGGATC TTGGACATCT AAAGGTGACA
    AGTAAAATTC GTTCAGAATT ACCAGATTTA AAACAAGGTG GAGCCAATCG TGAAGAGATA
    ATGCATAGAG CTTATGATTC CTTTGATATT CAACTTACAA GCATACAGCT GCTTTTCAGC
    AGAGTTGGTG ATAATTGGAA AGAAGCACGA AAGCTCAATG TGTCTGCACA GCATATCTTG
    GTACCTATGC ACTTCAATGT GGAGCTCTCT AAGGCCATGG TTTTCATGGA TATAAGTATG
    CCGAAATTCA AGATTTATGG AAACTTACCA TTAATTTCTT TACGAATCTC AGATAAAAAA
    CTGCAAGGGA TTTTGGAACT GATTGAAAGC ATTCCAAAAC CCTCACCTGC AACTGAAGTA
    AATGTGCCTG TCAAATCATC TCAGGTTCAA AGATCCACTT CTTTGGGAAC ATCACAGATG
    TCACAGAAGA AATTTCCTCT TTTGGAAATT CCATGTGTTG AAGATGATTC AGATGAGGAG
    TTTTTTGATG CACCATGTAG TCCGTTGGAA GAGCCTCTTC AATCACCATC TAGAATTAAA
    AGTCCTCAAC AGAAAAATCT TCAGAAACCA GATTGTTTTA AAAATATGAT TGAGTATAAA
    ATAAGATTTG AAGTGCCAGA GGTTTTGATT CAGTTCTGTC ACCTTGTTGG AGACTGTGAA
    CTACCCGTGG TAGAAATTAA TGTCTTAGGA TTGGGCACAG ATATTGAGCT TAGAACATTT
    GATTTGAAAG CAAATGCCTT TTTGAAAGAG GTCTGCTTAA AATGTCCAGA TTATTTTGAT
    GAAAACAAGA AACCAGTTTA TTTGATTACA ACACTGGATA ACACAATGGA AGATCTCTTA
    ACACTGGAAT ATGTGAAGGC TGAAAATAAT TTACCTGCAA CAATATTGAA GAATGCTTAT
    AACAGTGTTG TGCAACTGAT AAAGGTGAAT TTTTCCTCTT TGGATATTCA TTTACATACT
    GAAGCACTTT TGAATACAAT AAATTATTTT AATAATATCC TTCCATATTT GGAGGAAAAA
    CCAGCCCCAG TGCATGTTGT GGAGACTGAA GACAAAGAGG AGGTTGTTAA AAAAATACCT
    TTAAAATCAT CCAAGAATGA AGATACTATT ACCTTGCAGA TTTTAGCAGA ATTATCATGT
    TTACAGATCT TTATTCAAGA TCAGAAACGT AACATTTCCG AAATTAAGAT AGAAGGGCTT
    GATTGTGAAA TCATTATGAG GCCATCAGTG ACTGAAACAA ATGCAAAGCT AAGGAATATA
    ATTGTCCTGG ATTCTGATAT AACAGCTGTA TACAAAAAGG CTGTTTATAT TACCGGAAAA
    GAAGTTTTCA GCTTCAAAAT GGTTTCTTAT ATGGGTGCAA CTGCTGGTTC TGCATACACA
    GATATGAATG TAGTTGATAT GCAAGTTAAT TTAACTGTTG GTTGCATTGA AGTAGTTCTT
    GTCAAAAAAT TTCTGTATTC TATATTGGCT TTTATAGATA ATTTTCAAGC AGGTAAACAA
    GCCTTAGCGG AGGCAACTGT TCAGGCAGCA GGAATGGCTG CTACTGGTGT AAAAGAACTG
    GCACAGAGGA GTTCTAGAAT GGCATTGGAT GTTCACATCA AAGCCCCTGT TGTGGTTATC
    CCACAGTCTC CAGTTTCTGA AAATATTTTT GTTGCTGATT TTGGACTAAT TACAATGAAA
    AATACATTCC ATGTAATAAC AAAGAGTCCG ACCAACCCCC CACCCATTAT TGATTTGATA
    ACGATAAAGC TAAGTGAAAT GCGACTATAC AGGACTCAAT ATATTAATGA TGCATACCAG
    GAAGTACTGG ATCTACTATT GCCATTAAAT CTTGAGGTGA TGGTTGAGCG AAATTTATCG
    TGGGAGTGGT TCCAGGAAGT TCCTTGTTTC AATCTAAATG CTCAGCTAAA GCCCATGGAG
    TTCATTCTTA GTCAAGAAGA TATAACGACC ATTTTTAAAA CATTATATGG CAACATATGG
    TATGAAGGTG ATAGTGCTTC ACATGAGGTA ACAAAGGATC AGTCCAGTGT TACTAGTGGT
    GCTACTACTG CGTCACACCA TGCGGCAGGA ACAACTGTAG TGACAGCTGC TGTGGTAGAA
    GTACATTCAC ATGCTTCTCA AGTTAAGACA ACACTAAATA TGAGTTTCAG AACCGATTAT
    CTCACCATGG TACTGTATAG TCCAGGTCCT AAACAGACTT CCTTTACAGA TATTCGTGAT
    CCTTCTCTGA AACTTGCTGA ATTTAAGTTG GAGAATATTA TAAGCACTTT AAAAATGTAT
    ACAGATGACT CAATATTTTC TTCCTTCTCA TTAAAAAACT GTATTTTAGA TGACAAAAGG
    CCTCATATCA AGAAGGCAAC TCCTAGAATG ATAGGACTGA CAGTTGGGTT TGACAAAAAG
    GACATGATGG ATGTAAGGTA TAGGACGATC CGAGATGGTT GGGTGGCTGA TATAGTCCTT
    CAAGAAATGT ATATTTGTGC AAGTGTGGAA TTTCTGCTGA CAGTTGCAAA TGTCTTTCTT
    GAGGCCTACA CTACAGGCAC TGCTGTAGAA ACCAATGTTC AAACATGGTC TGCCAAAGAA
    GAAGTACCTA AACAGCAATC AGAAAAGTGG GAAATGAACA TCACTATTAA AAATCCTGAA
    ATTGTGTTTG TCGCTGATAT GACAAGAAAT GATGCTCCTG CCTTAGTCAT TACAACACAG
    TGTGAAATAT ATTGGCTAGG TGACTTTGAA AATGATACAA TGACTGCTGC CATTAAAGAT
    CTCCAAGTGA GAGCATGCCC ATTTCTTCCA GTCAAGAGAA AAGGAAAAAT CACTACTGTT
    TTGCAACCCT GTGATTTGTT CTATCAAGCT ACTCAGATAA GTACAGATCC ACAAGTACTT
    GAATTATCAG TAAAATCCCT GACACTGAAG GTTTCACCAG TTATCATAAA TACTGTGATT
    ACCATAACTT CAGCACTTTA TACTGCTAAG GAAACCATCC CAAAAGAAAC TGCTTCTTCT
    ACAGCACATT TATGGGAAAA GAAGGATACA AAAGATTTAA ATATGTGGTT TCTTGAAGAA
    CCAAATGAAG CTGTAAAAAG AGCTCCCATG CCTGAATTGG TACCCAAAGG AGAGATAATA
    AAAATGAACA TTGATTCTAT TTTTCTGGTT CTTGAGGCTG GAATTGGTCA TAGAACAGTA
    CCTATGCTAT TGGCAAAATC ACGTTTCTCA GGGGAAGGCA AAAACTGGAG TTCCTTAATA
    AATCTCCACT GTCAGCTTGA GCTAGAAGTA CATTATTACA ATGAAATGTT TGGTGTGTGG
    GAGCCTTTGC TTGAACCCTT AGAAATTGAT CAGACTGAGG ATTTTAGACC ATGGAATCTT
    GGAATCAAGA TGAAAAAGAA AGCCAAAAAG GCCATTGTTG AGTCAGGTAC TGAAGAAGAA
    AATTACAGAG TACCAGAATA CAAAACTGTC ATCACTTTCT ATTCGAAAGA CCAGCTAAAC
    ATTACATTAT CTAAATGTGG CCTTATAATG TTAAATAATT TAGCTGAGGC ATTTACAGAA
    GCTGCCACTG GATCTTCAGC TACCTTCATA AGGGATCTGG CACCTTTTAT GATTTTAAAT
    TCTCTTGGAC TTAGTATTTC TGTTTCACCA AGTGAATCTT TTAGTGTACT CAACGTACCT
    ATGGCAAAAT CATATGTATT GAAAAATGAG GAGAGTTTAA GTATGGATTA TATGCGAACT
    AAAGACAATG ATCATTTCAA TGCAATGACC AGCCTAAGCA GCAAACACTT CTTCATTCTT
    CTTACCCCTG CTAACCACTC TACTGCTGAT AAAATCCCTT TAACAAAAGT GGGACGACGA
    CTGTACACTG TAAGACACAG AGAGTCTGGA GTTGAAAGAT CAATTGTTTG TCAGATTGAT
    GCAGTGGAAG GAAGTAAGAA GGTAACAGTT CGCTCTCCAG TGCAGATTAG AAATCATTTT
    TCAATACCAC TTTCTGTTTA TGGAGGAGAC ACGTTATTGG GAACGGCCTC ACCTGGAAGT
    GAATTTAACA TACCATTAGC ATCTTACCGA TCACTACTTT CTCTGAAGCC AGAAGATGCA
    GACTATCAGA TGTGTGAAGG AATTGACTTT GAAGAAATTA TAAAACAAGA TGGTACTTCT
    TTCAAGAAAA AATGTAGACC TATAAACCCT TCCAGGAAGC CATTTATCAT TAATATAGTC
    CCAGAAAAAG ATAATTTGAC GTATATGTCA GTGTATTCAG AAGACCGTTG GGACTTACCA
    TATATAATAC ATTTGTGGCC ACCTATTCTA CTCCGAAATC TTCTTCCTTA CAAAATTGCT
    TATTACATAG AGGGGATTGA ACATAGAGTT TTTACTCTCC ATGAAGGCCG TTCAGCCCAG
    CTTTGTACTG TACAGTTGGA TAAAGCCAGG CTACATTTAA AATTACTTGA CTATCTTAAT
    CATGATTGGA AAAGTGAATA TCATATAAAA CCTAATCAAC AAGATATTAG TTTCATCAGT
    TTTACTTGTG TTACAGAAAT AGAAAAGACT GATTTAGATA TTGCTGTCCA TGTGACTTAT
    AATACTGGCC AGACTATTAT GGCATTTCAT AGTCCTTATT GGATGGTTAA TAAAACTGGT
    CGAATGTTGC AGTATAAAGC AGATGGAATT CATCGAAAGC ATCCACCTAA TTATAAAAAG
    CCAGTTCTCT TTTCATTTCA GCCAAAGCAC TTTTTCAATA ACAATAAGGT TCAGCTTATG
    GTAACTGATA GTGAATTGTC AGATCAATTT TCAATTGATA CAGTTGGTAG TCATGGAGCG
    GTTAAATGTA AAGGCCCAAA AATGGAATAT CAAGTTGGTG TCACTATAGA CCTTAGCAGT
    TTTAACATTA CCAGAATTGT CACATTTACC CCCTTTTTTT TGATTAAAAA CAAAAGCAAA
    TATCATATAT CAGTGGCTGA AGAGGGAAAT GATAAATGGC TCGCTCTTGA TTTGGAGCAG
    TGTGTCCCCT TTTGGCCTGA GGATGCTTCT AATAAACTTC TTATTCGAGT TGAAAGAAGT
    GAAAGCCCTC CCCATAAGAT ACATTTTAAT AAGGAAGAAA GTTGTATTTT ACTGCGTCTA
    GACAATGAGC TTGGAGGTAT TATAGCAGAA GTTAATTTGG CTGAGCATTC TACAGTTATT
    ACATTTTCAG ATTATCGTGA TGGAGCCGCT ACATTTCTGT TAATAAATCA CACCAAGAAT
    GACATTGTTC AGTATAAGCA AAGTTCCCTC AGTGAAATAG AAGATTCCCT CCCTCCTGGA
    AAAGCAGTAT TTTATACATG GGCTGATCCA GTGGGCTCTA GAAAGCTGCA GTGGAAATGT
    GGAAAAAGCC ACGGTGAAGT AACACAAAAG GATGATATGA TGACACCTAT AAATTTGGGG
    AAAACGACCA TTTATTTAGT TTCATTTTTT GAAGGCTTAC AACGCATAAT TTTATTCACT
    GAAGATCCAA GAGTATTTAA AGTGACATAT GAAAGTGAGA AAGCAGAGTT AGCAGAGCAA
    GAAATTGTGT TGGCGTTACA AGATGTTGGG ATTTCTCTTG TCAACAATTA TACAAAGCAA
    GAAGTAGCCT ATATTGGCAT TACAAGTTCT GATGTGGTTT GGGAGACAAA GCCCAGGAAG
    AAGGCAAGAT GGAAGCCAAT GAGTGTAAAG CACACTGAGA AGTTAGAGAA GGAATTTAAG
    GAATATGTTG AATCTTCACC GTCAGAAGAT AAGGTTATTG AGTTGGACAC CAACATTCTG
    GTTCGCTTCA CACCTGGTGC TTCTAACATG AAAATTCTGC AGCCACATGT AATAGCTATA
    AGAAGAAATT ATCTTCCAGC ATTAAAAGTA GAATATAGCA CATCTGCACA TCAGTCATCG
    TTTAGAATTC AAATTTATAG AATACAGATC CAAAACCAAA TACATGGTGC TATATTTCCA
    TTCGTGTTTT ATCCCATTAA ACCGCCCAAG TCCGTCACCT TGGATTCAGC ACCGAAACCC
    TTTACTGATG TCAGTATTGT CATGAGATCT GCAGGACATT CGCAGATATC ACGTATTAAG
    TATTTCAAAG TGTTGATCCA AGAAATGGAT CTCAGGTTAG ATCTTGGATT TGTCTATGCT
    GTATCAGAAC TTATGACACC AGCTGAAGTC ACTGAAAAAA CAGAGGTTGA GCTTTTTCAT
    AAAGACATAG AAGCTTTCCA AGAAGAATAT AAAATAGTCT CATTAGTAGA TTCATCACAA
    GTCAGTCTCT ATGAATACTT TCATATATCT CCTATCAAGT TGCATTTAAG TGTTTCACTG
    AGTTCAGGTG GTGAAGAACC GAAAGATTCA GAACAGCATG AAGGACTGAT GGCAATTCGT
    TCTTTACATC TTTTGCTGAA GAGTATTGGT GCCACTCTCA CAGATGTACA AGATGTAGTT
    TTTAAGCTAG CATTTTTTGA ACTCAATTAT CAGTTCCATA CAACAGCTGA ACTACAGTCT
    GAAGTAATAA GACACTATTC AAAGCAGGCC ATTAAACAGA TGTACGTACT CATTCTTGGA
    CTTGATGTTT TGGGAAATCC CTTTGGCTTG ATAAGAGAAT TTTCTGAAGG TGTAGAAGCA
    TTTTTTTATG AACCTTACCA GGGAGCCATC CAGGGTCCTG AAGAGTTTGT GGAAGGAATG
    GCACTAGGAC TGAAAGCACT AGTTGGTGGA GCTGTTGGTG GACTAGCTGG TGCTGCCTCC
    AAAATCACCA GTGCTATGGC TAAAGGGGTG GCAGCTATGA CTATGGATGA AGACTACCAA
    CAGAAGAGAA GAGAAGCCAT GAATAAGCAA CCAGCTGGTC TTAGGGAAGG CATCACTCGT
    GGAGGAAAAG GCCTAGTTTC TGGTTTTGTA AGTGGCATAA CAGGAATTGT TACAAAACCA
    ATCAAAGGAG CTCAGAAAGA AGGAGCAACT GGTTTCTTTA AAGGTGTTGG GAAAGGTTTA
    GTTGGAGCAG TGACAAGGCC AACTGGAGGC ATCATAGACA TGGCTAGCAG TACATTTCAG
    GGAATAAAAA GAGCTACAGA GACCTCTGAT GAAGTGGAGA GTCTGCGACC CCCTAGATTC
    TTTAATGAAG ATGGAGTTAT CAGACCTTAC AGATTGAGGG ATGGTACTGG AAATCAAATG
    TTACAGGTCA TGGAAAACGG AAGATTTGCA AAATACAAAT ATTTTACCCA TGTCATGATC
    AATAAGACAG ACATGTTAAT GATTACCAGA AGTGGTGTGT TGTTTGTAAC AAAAGGAACA
    TTTGGACAGC TTACTTGTGA GTGGCAGTAT AGTTTTGATG AGTTTACCAA AGAGCCATTC
    ATTGTTCATG AGAGAAGACT ACGCATCGAA GCAAAGGAAC GGGTGAAGTC TGTATTCCAT
    GCCAAAGAGT TTGGAAAAAT AGTTAACTTC AGAACCCCGG AGGATGCGAG GTGGATCCTC
    AGAAAGCTAG AAGAAGCAAG AGAACCTTCC CCGAACCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237445
    Clone ID Related Accession (Same CDS sequence) XM_024996187.1 , XM_024996188.1
    Accession Version XM_024996188.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product vacuolar protein sorting-associated protein 13A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    481
    541
    601
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    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2521
    2581
    2641
    2701
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    3001
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    3181
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    3421
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    3721
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    4141
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    4261
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    4381
    4441
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    5881
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    7141
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    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGGTGTTCG AGTCGGTGGT CGTGGACGTG TTGAACCGGT TCTTGGGGGA CTATGTGGTG 
    AACCTGGACA CGTCCCAGCT CAGTCTGGGC ATCTGGGCAG GAGCTGTGGC CCTGAAGAAT
    CTTGAAATTA AAGAAAATGC CCTGAGTCAA CTGGACGTAC CATTTAAAAT TAAAGTTGGC
    CATATAGGTA CTCTTAATCT TATAATTCCA TGGAAAAACC TTTATACCCA ACCAGTTGAA
    GCTGTATTGG AAGGCGTTTA TTTACTCATA GTGCCTTCTT CTAGAATAAA ATATGATCCT
    ATAAAAGAAG AAAAACAACT CTTTGAAGCA AAGCAACAGG AATTGAAAAG AATAGAAGAA
    GCAAAACGAA AAGTAGCTGA TCAAGAAAAA CAGCTTGTGG AGAAACAGGA CACTTTCACA
    GAAAAATTAA TTACCCAGAT CATAAAAAAT CTTCAAGTGA AAATTTCCAA CATCCATATT
    CGCTATGAAG ATGATGTCAC AAATCGAGAA AACCCTCTGT CATTTGGTAT TTCCCTTCAG
    AATCTCAGCA TGCAGACAAC TGATCAATAC TGGATTCCAT GTTTACATGA TGAAACTGAG
    AAATTATTTC GTAAGTTAAT CCGATTGGAT AACCTTTTTG CCTATTGGAA TGTGAAGACT
    CAGTTGTTTT ATCTTAGTAA TTATGATGAA TCAGTGAGCA GCCTGAGGAA TGGCATTGTT
    GATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTATTTC GCCCCATATC TGCCAATGCC
    AAACTTCAGA TGAATCGCAG ATCGGATTTT GACTTTTCTG CCCCCAAAAT AGACCTGGAT
    GTTGAGTTAC ATGACATAAC AATTCAACTT AATAAACCAC AGTATTTCAG TTTTATGGAA
    CTTCTTGAAT CGGTTGATAT GATGACGCAA AATGTACCAT ATAGGAAATT CAAACCTGAT
    GTACCTCTTC GTTACCATGC CAAAGAATGG TGGGCTTATG CTATACATGG TATCCTTGAA
    GTAAACATTC GCCCCCACTT ACGGATGTGG TCCTGGAAGC ACATTAGAGA ACATAGGCAA
    AAAATGAAGC AATATAAAGA ACTGTATAAA AAAAAGCTAA CAAGTAAGAA GCCATCTGGT
    GAACTTCTCA ACTCTTTGGA GGATTTGGAA AAGACTTTGG ATGTGTTTAA TATAACTGTA
    GCAAGACAAC AGGCGGAAGT TGAGGTAAAG AAAGCTGGAT ACAGGATTTT CAGAGAAGGA
    GCAGAAGAGT CCGAGGGCAA TAAAGGATGG TTTAGCTGGA TGTGGAGTTG GTCAGAATCG
    CATAGTAAAC AACAACTAGA TATTAAACCT GGAAGTCTTG AAGAAATATT GACACCTAAA
    GAAAAAAGTT TGCTGTATGA AGCAATTGGC TATAGTGAAA CAGCAGTTGA TCCAACCTTG
    CCAAAAACAT TTGAAGCAAT GAAGTTCTTT GTTCACTTGA AAAGTATGTC TATTATTCTA
    AGAGAACAAC AACAAAAACC TGAACTGCTA GATATTGTAG TGGAAGAATT TAGTACTTTG
    ATTGTGCAAA GACCAGGAGC ACAAGCAGTT AAATTTGAAA CCAAAATAGA TTCATTTCAT
    GTTACTGGCT TACCAGATAA CTCAACAAAA CCCCGCCTCC TGTCTTCATT AGATGATGCT
    GCATCACTCT TGAAAATTAT GTTTGAGATA AATCCTTTAG ATGAAACTGT TGCTCAGAGG
    TGTATCATAG AAGCTGAACC TTTAGAAATG TTATATGATG CAAGAACAGT GAATAGTATA
    GTAGAATTCT TCAGACCTCC AAAAGAGGTA AATCTAGCAC AGCTGACTTC AGTAACTTTG
    ACAAAACTTG AAGAATTTCG TGATAAGACA GCAACAGGTC TGGTATATAT TATTGAAACA
    CAGAAAGTTC TTGATCTCAA AATTAACTTG AATGCTTCAT ATATCATTAT CCCACAAGAT
    GGTATCTTTA GCCCTACGTC AAATCTCCTT CTTTTGGATC TTGGACATCT AAAGGTGACA
    AGTAAAATTC GTTCAGAATT ACCAGATTTA AAACAAGGTG GAGCCAATCG TGAAGAGATA
    ATGCATAGAG CTTATGATTC CTTTGATATT CAACTTACAA GCATACAGCT GCTTTTCAGC
    AGAGTTGGTG ATAATTGGAA AGAAGCACGA AAGCTCAATG TGTCTGCACA GCATATCTTG
    GTACCTATGC ACTTCAATGT GGAGCTCTCT AAGGCCATGG TTTTCATGGA TATAAGTATG
    CCGAAATTCA AGATTTATGG AAACTTACCA TTAATTTCTT TACGAATCTC AGATAAAAAA
    CTGCAAGGGA TTTTGGAACT GATTGAAAGC ATTCCAAAAC CCTCACCTGC AACTGAAGTA
    AATGTGCCTG TCAAATCATC TCAGGTTCAA AGATCCACTT CTTTGGGAAC ATCACAGATG
    TCACAGAAGA AATTTCCTCT TTTGGAAATT CCATGTGTTG AAGATGATTC AGATGAGGAG
    TTTTTTGATG CACCATGTAG TCCGTTGGAA GAGCCTCTTC AATCACCATC TAGAATTAAA
    AGTCCTCAAC AGAAAAATCT TCAGAAACCA GATTGTTTTA AAAATATGAT TGAGTATAAA
    ATAAGATTTG AAGTGCCAGA GGTTTTGATT CAGTTCTGTC ACCTTGTTGG AGACTGTGAA
    CTACCCGTGG TAGAAATTAA TGTCTTAGGA TTGGGCACAG ATATTGAGCT TAGAACATTT
    GATTTGAAAG CAAATGCCTT TTTGAAAGAG GTCTGCTTAA AATGTCCAGA TTATTTTGAT
    GAAAACAAGA AACCAGTTTA TTTGATTACA ACACTGGATA ACACAATGGA AGATCTCTTA
    ACACTGGAAT ATGTGAAGGC TGAAAATAAT TTACCTGCAA CAATATTGAA GAATGCTTAT
    AACAGTGTTG TGCAACTGAT AAAGGTGAAT TTTTCCTCTT TGGATATTCA TTTACATACT
    GAAGCACTTT TGAATACAAT AAATTATTTT AATAATATCC TTCCATATTT GGAGGAAAAA
    CCAGCCCCAG TGCATGTTGT GGAGACTGAA GACAAAGAGG AGGTTGTTAA AAAAATACCT
    TTAAAATCAT CCAAGAATGA AGATACTATT ACCTTGCAGA TTTTAGCAGA ATTATCATGT
    TTACAGATCT TTATTCAAGA TCAGAAACGT AACATTTCCG AAATTAAGAT AGAAGGGCTT
    GATTGTGAAA TCATTATGAG GCCATCAGTG ACTGAAACAA ATGCAAAGCT AAGGAATATA
    ATTGTCCTGG ATTCTGATAT AACAGCTGTA TACAAAAAGG CTGTTTATAT TACCGGAAAA
    GAAGTTTTCA GCTTCAAAAT GGTTTCTTAT ATGGGTGCAA CTGCTGGTTC TGCATACACA
    GATATGAATG TAGTTGATAT GCAAGTTAAT TTAACTGTTG GTTGCATTGA AGTAGTTCTT
    GTCAAAAAAT TTCTGTATTC TATATTGGCT TTTATAGATA ATTTTCAAGC AGGTAAACAA
    GCCTTAGCGG AGGCAACTGT TCAGGCAGCA GGAATGGCTG CTACTGGTGT AAAAGAACTG
    GCACAGAGGA GTTCTAGAAT GGCATTGGAT GTTCACATCA AAGCCCCTGT TGTGGTTATC
    CCACAGTCTC CAGTTTCTGA AAATATTTTT GTTGCTGATT TTGGACTAAT TACAATGAAA
    AATACATTCC ATGTAATAAC AAAGAGTCCG ACCAACCCCC CACCCATTAT TGATTTGATA
    ACGATAAAGC TAAGTGAAAT GCGACTATAC AGGACTCAAT ATATTAATGA TGCATACCAG
    GAAGTACTGG ATCTACTATT GCCATTAAAT CTTGAGGTGA TGGTTGAGCG AAATTTATCG
    TGGGAGTGGT TCCAGGAAGT TCCTTGTTTC AATCTAAATG CTCAGCTAAA GCCCATGGAG
    TTCATTCTTA GTCAAGAAGA TATAACGACC ATTTTTAAAA CATTATATGG CAACATATGG
    TATGAAGGTG ATAGTGCTTC ACATGAGGTA ACAAAGGATC AGTCCAGTGT TACTAGTGGT
    GCTACTACTG CGTCACACCA TGCGGCAGGA ACAACTGTAG TGACAGCTGC TGTGGTAGAA
    GTACATTCAC ATGCTTCTCA AGTTAAGACA ACACTAAATA TGAGTTTCAG AACCGATTAT
    CTCACCATGG TACTGTATAG TCCAGGTCCT AAACAGACTT CCTTTACAGA TATTCGTGAT
    CCTTCTCTGA AACTTGCTGA ATTTAAGTTG GAGAATATTA TAAGCACTTT AAAAATGTAT
    ACAGATGACT CAATATTTTC TTCCTTCTCA TTAAAAAACT GTATTTTAGA TGACAAAAGG
    CCTCATATCA AGAAGGCAAC TCCTAGAATG ATAGGACTGA CAGTTGGGTT TGACAAAAAG
    GACATGATGG ATGTAAGGTA TAGGACGATC CGAGATGGTT GGGTGGCTGA TATAGTCCTT
    CAAGAAATGT ATATTTGTGC AAGTGTGGAA TTTCTGCTGA CAGTTGCAAA TGTCTTTCTT
    GAGGCCTACA CTACAGGCAC TGCTGTAGAA ACCAATGTTC AAACATGGTC TGCCAAAGAA
    GAAGTACCTA AACAGCAATC AGAAAAGTGG GAAATGAACA TCACTATTAA AAATCCTGAA
    ATTGTGTTTG TCGCTGATAT GACAAGAAAT GATGCTCCTG CCTTAGTCAT TACAACACAG
    TGTGAAATAT ATTGGCTAGG TGACTTTGAA AATGATACAA TGACTGCTGC CATTAAAGAT
    CTCCAAGTGA GAGCATGCCC ATTTCTTCCA GTCAAGAGAA AAGGAAAAAT CACTACTGTT
    TTGCAACCCT GTGATTTGTT CTATCAAGCT ACTCAGATAA GTACAGATCC ACAAGTACTT
    GAATTATCAG TAAAATCCCT GACACTGAAG GTTTCACCAG TTATCATAAA TACTGTGATT
    ACCATAACTT CAGCACTTTA TACTGCTAAG GAAACCATCC CAAAAGAAAC TGCTTCTTCT
    ACAGCACATT TATGGGAAAA GAAGGATACA AAAGATTTAA ATATGTGGTT TCTTGAAGAA
    CCAAATGAAG CTGTAAAAAG AGCTCCCATG CCTGAATTGG TACCCAAAGG AGAGATAATA
    AAAATGAACA TTGATTCTAT TTTTCTGGTT CTTGAGGCTG GAATTGGTCA TAGAACAGTA
    CCTATGCTAT TGGCAAAATC ACGTTTCTCA GGGGAAGGCA AAAACTGGAG TTCCTTAATA
    AATCTCCACT GTCAGCTTGA GCTAGAAGTA CATTATTACA ATGAAATGTT TGGTGTGTGG
    GAGCCTTTGC TTGAACCCTT AGAAATTGAT CAGACTGAGG ATTTTAGACC ATGGAATCTT
    GGAATCAAGA TGAAAAAGAA AGCCAAAAAG GCCATTGTTG AGTCAGGTAC TGAAGAAGAA
    AATTACAGAG TACCAGAATA CAAAACTGTC ATCACTTTCT ATTCGAAAGA CCAGCTAAAC
    ATTACATTAT CTAAATGTGG CCTTATAATG TTAAATAATT TAGCTGAGGC ATTTACAGAA
    GCTGCCACTG GATCTTCAGC TACCTTCATA AGGGATCTGG CACCTTTTAT GATTTTAAAT
    TCTCTTGGAC TTAGTATTTC TGTTTCACCA AGTGAATCTT TTAGTGTACT CAACGTACCT
    ATGGCAAAAT CATATGTATT GAAAAATGAG GAGAGTTTAA GTATGGATTA TATGCGAACT
    AAAGACAATG ATCATTTCAA TGCAATGACC AGCCTAAGCA GCAAACACTT CTTCATTCTT
    CTTACCCCTG CTAACCACTC TACTGCTGAT AAAATCCCTT TAACAAAAGT GGGACGACGA
    CTGTACACTG TAAGACACAG AGAGTCTGGA GTTGAAAGAT CAATTGTTTG TCAGATTGAT
    GCAGTGGAAG GAAGTAAGAA GGTAACAGTT CGCTCTCCAG TGCAGATTAG AAATCATTTT
    TCAATACCAC TTTCTGTTTA TGGAGGAGAC ACGTTATTGG GAACGGCCTC ACCTGGAAGT
    GAATTTAACA TACCATTAGC ATCTTACCGA TCACTACTTT CTCTGAAGCC AGAAGATGCA
    GACTATCAGA TGTGTGAAGG AATTGACTTT GAAGAAATTA TAAAACAAGA TGGTACTTCT
    TTCAAGAAAA AATGTAGACC TATAAACCCT TCCAGGAAGC CATTTATCAT TAATATAGTC
    CCAGAAAAAG ATAATTTGAC GTATATGTCA GTGTATTCAG AAGACCGTTG GGACTTACCA
    TATATAATAC ATTTGTGGCC ACCTATTCTA CTCCGAAATC TTCTTCCTTA CAAAATTGCT
    TATTACATAG AGGGGATTGA ACATAGAGTT TTTACTCTCC ATGAAGGCCG TTCAGCCCAG
    CTTTGTACTG TACAGTTGGA TAAAGCCAGG CTACATTTAA AATTACTTGA CTATCTTAAT
    CATGATTGGA AAAGTGAATA TCATATAAAA CCTAATCAAC AAGATATTAG TTTCATCAGT
    TTTACTTGTG TTACAGAAAT AGAAAAGACT GATTTAGATA TTGCTGTCCA TGTGACTTAT
    AATACTGGCC AGACTATTAT GGCATTTCAT AGTCCTTATT GGATGGTTAA TAAAACTGGT
    CGAATGTTGC AGTATAAAGC AGATGGAATT CATCGAAAGC ATCCACCTAA TTATAAAAAG
    CCAGTTCTCT TTTCATTTCA GCCAAAGCAC TTTTTCAATA ACAATAAGGT TCAGCTTATG
    GTAACTGATA GTGAATTGTC AGATCAATTT TCAATTGATA CAGTTGGTAG TCATGGAGCG
    GTTAAATGTA AAGGCCCAAA AATGGAATAT CAAGTTGGTG TCACTATAGA CCTTAGCAGT
    TTTAACATTA CCAGAATTGT CACATTTACC CCCTTTTTTT TGATTAAAAA CAAAAGCAAA
    TATCATATAT CAGTGGCTGA AGAGGGAAAT GATAAATGGC TCGCTCTTGA TTTGGAGCAG
    TGTGTCCCCT TTTGGCCTGA GGATGCTTCT AATAAACTTC TTATTCGAGT TGAAAGAAGT
    GAAAGCCCTC CCCATAAGAT ACATTTTAAT AAGGAAGAAA GTTGTATTTT ACTGCGTCTA
    GACAATGAGC TTGGAGGTAT TATAGCAGAA GTTAATTTGG CTGAGCATTC TACAGTTATT
    ACATTTTCAG ATTATCGTGA TGGAGCCGCT ACATTTCTGT TAATAAATCA CACCAAGAAT
    GACATTGTTC AGTATAAGCA AAGTTCCCTC AGTGAAATAG AAGATTCCCT CCCTCCTGGA
    AAAGCAGTAT TTTATACATG GGCTGATCCA GTGGGCTCTA GAAAGCTGCA GTGGAAATGT
    GGAAAAAGCC ACGGTGAAGT AACACAAAAG GATGATATGA TGACACCTAT AAATTTGGGG
    AAAACGACCA TTTATTTAGT TTCATTTTTT GAAGGCTTAC AACGCATAAT TTTATTCACT
    GAAGATCCAA GAGTATTTAA AGTGACATAT GAAAGTGAGA AAGCAGAGTT AGCAGAGCAA
    GAAATTGTGT TGGCGTTACA AGATGTTGGG ATTTCTCTTG TCAACAATTA TACAAAGCAA
    GAAGTAGCCT ATATTGGCAT TACAAGTTCT GATGTGGTTT GGGAGACAAA GCCCAGGAAG
    AAGGCAAGAT GGAAGCCAAT GAGTGTAAAG CACACTGAGA AGTTAGAGAA GGAATTTAAG
    GAATATGTTG AATCTTCACC GTCAGAAGAT AAGGTTATTG AGTTGGACAC CAACATTCTG
    GTTCGCTTCA CACCTGGTGC TTCTAACATG AAAATTCTGC AGCCACATGT AATAGCTATA
    AGAAGAAATT ATCTTCCAGC ATTAAAAGTA GAATATAGCA CATCTGCACA TCAGTCATCG
    TTTAGAATTC AAATTTATAG AATACAGATC CAAAACCAAA TACATGGTGC TATATTTCCA
    TTCGTGTTTT ATCCCATTAA ACCGCCCAAG TCCGTCACCT TGGATTCAGC ACCGAAACCC
    TTTACTGATG TCAGTATTGT CATGAGATCT GCAGGACATT CGCAGATATC ACGTATTAAG
    TATTTCAAAG TGTTGATCCA AGAAATGGAT CTCAGGTTAG ATCTTGGATT TGTCTATGCT
    GTATCAGAAC TTATGACACC AGCTGAAGTC ACTGAAAAAA CAGAGGTTGA GCTTTTTCAT
    AAAGACATAG AAGCTTTCCA AGAAGAATAT AAAATAGTCT CATTAGTAGA TTCATCACAA
    GTCAGTCTCT ATGAATACTT TCATATATCT CCTATCAAGT TGCATTTAAG TGTTTCACTG
    AGTTCAGGTG GTGAAGAACC GAAAGATTCA GAACAGCATG AAGGACTGAT GGCAATTCGT
    TCTTTACATC TTTTGCTGAA GAGTATTGGT GCCACTCTCA CAGATGTACA AGATGTAGTT
    TTTAAGCTAG CATTTTTTGA ACTCAATTAT CAGTTCCATA CAACAGCTGA ACTACAGTCT
    GAAGTAATAA GACACTATTC AAAGCAGGCC ATTAAACAGA TGTACGTACT CATTCTTGGA
    CTTGATGTTT TGGGAAATCC CTTTGGCTTG ATAAGAGAAT TTTCTGAAGG TGTAGAAGCA
    TTTTTTTATG AACCTTACCA GGGAGCCATC CAGGGTCCTG AAGAGTTTGT GGAAGGAATG
    GCACTAGGAC TGAAAGCACT AGTTGGTGGA GCTGTTGGTG GACTAGCTGG TGCTGCCTCC
    AAAATCACCA GTGCTATGGC TAAAGGGGTG GCAGCTATGA CTATGGATGA AGACTACCAA
    CAGAAGAGAA GAGAAGCCAT GAATAAGCAA CCAGCTGGTC TTAGGGAAGG CATCACTCGT
    GGAGGAAAAG GCCTAGTTTC TGGTTTTGTA AGTGGCATAA CAGGAATTGT TACAAAACCA
    ATCAAAGGAG CTCAGAAAGA AGGAGCAACT GGTTTCTTTA AAGGTGTTGG GAAAGGTTTA
    GTTGGAGCAG TGACAAGGCC AACTGGAGGC ATCATAGACA TGGCTAGCAG TACATTTCAG
    GGAATAAAAA GAGCTACAGA GACCTCTGAT GAAGTGGAGA GTCTGCGACC CCCTAGATTC
    TTTAATGAAG ATGGAGTTAT CAGACCTTAC AGATTGAGGG ATGGTACTGG AAATCAAATG
    TTACAGGTCA TGGAAAACGG AAGATTTGCA AAATACAAAT ATTTTACCCA TGTCATGATC
    AATAAGACAG ACATGTTAAT GATTACCAGA AGTGGTGTGT TGTTTGTAAC AAAAGGAACA
    TTTGGACAGC TTACTTGTGA GTGGCAGTAT AGTTTTGATG AGTTTACCAA AGAGCCATTC
    ATTGTTCATG AGAGAAGACT ACGCATCGAA GCAAAGGAAC GGGTGAAGTC TGTATTCCAT
    GCCAAAGAGT TTGGAAAAAT AGTTAACTTC AGAACCCCGG AGGATGCGAG GTGGATCCTC
    AGAAAGCTAG AAGAAGCAAG AGAACCTTCC CCGAACCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851956.1
    CDS235..9756
    Translation

    Target ORF information:

    RefSeq Version XM_024996188.1
    Organism Bos taurus(cattle)
    Definition Bos taurus vacuolar protein sorting 13 homolog A (VPS13A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996188.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGGTGTTCG AGTCGGTGGT CGTGGACGTG TTGAACCGGT TCTTGGGGGA CTATGTGGTG 
    AACCTGGACA CGTCCCAGCT CAGTCTGGGC ATCTGGGCAG GAGCTGTGGC CCTGAAGAAT
    CTTGAAATTA AAGAAAATGC CCTGAGTCAA CTGGACGTAC CATTTAAAAT TAAAGTTGGC
    CATATAGGTA CTCTTAATCT TATAATTCCA TGGAAAAACC TTTATACCCA ACCAGTTGAA
    GCTGTATTGG AAGGCGTTTA TTTACTCATA GTGCCTTCTT CTAGAATAAA ATATGATCCT
    ATAAAAGAAG AAAAACAACT CTTTGAAGCA AAGCAACAGG AATTGAAAAG AATAGAAGAA
    GCAAAACGAA AAGTAGCTGA TCAAGAAAAA CAGCTTGTGG AGAAACAGGA CACTTTCACA
    GAAAAATTAA TTACCCAGAT CATAAAAAAT CTTCAAGTGA AAATTTCCAA CATCCATATT
    CGCTATGAAG ATGATGTCAC AAATCGAGAA AACCCTCTGT CATTTGGTAT TTCCCTTCAG
    AATCTCAGCA TGCAGACAAC TGATCAATAC TGGATTCCAT GTTTACATGA TGAAACTGAG
    AAATTATTTC GTAAGTTAAT CCGATTGGAT AACCTTTTTG CCTATTGGAA TGTGAAGACT
    CAGTTGTTTT ATCTTAGTAA TTATGATGAA TCAGTGAGCA GCCTGAGGAA TGGCATTGTT
    GATGAAAATA TTGTTCCAGA AGGTTATGAT TTTGTATTTC GCCCCATATC TGCCAATGCC
    AAACTTCAGA TGAATCGCAG ATCGGATTTT GACTTTTCTG CCCCCAAAAT AGACCTGGAT
    GTTGAGTTAC ATGACATAAC AATTCAACTT AATAAACCAC AGTATTTCAG TTTTATGGAA
    CTTCTTGAAT CGGTTGATAT GATGACGCAA AATGTACCAT ATAGGAAATT CAAACCTGAT
    GTACCTCTTC GTTACCATGC CAAAGAATGG TGGGCTTATG CTATACATGG TATCCTTGAA
    GTAAACATTC GCCCCCACTT ACGGATGTGG TCCTGGAAGC ACATTAGAGA ACATAGGCAA
    AAAATGAAGC AATATAAAGA ACTGTATAAA AAAAAGCTAA CAAGTAAGAA GCCATCTGGT
    GAACTTCTCA ACTCTTTGGA GGATTTGGAA AAGACTTTGG ATGTGTTTAA TATAACTGTA
    GCAAGACAAC AGGCGGAAGT TGAGGTAAAG AAAGCTGGAT ACAGGATTTT CAGAGAAGGA
    GCAGAAGAGT CCGAGGGCAA TAAAGGATGG TTTAGCTGGA TGTGGAGTTG GTCAGAATCG
    CATAGTAAAC AACAACTAGA TATTAAACCT GGAAGTCTTG AAGAAATATT GACACCTAAA
    GAAAAAAGTT TGCTGTATGA AGCAATTGGC TATAGTGAAA CAGCAGTTGA TCCAACCTTG
    CCAAAAACAT TTGAAGCAAT GAAGTTCTTT GTTCACTTGA AAAGTATGTC TATTATTCTA
    AGAGAACAAC AACAAAAACC TGAACTGCTA GATATTGTAG TGGAAGAATT TAGTACTTTG
    ATTGTGCAAA GACCAGGAGC ACAAGCAGTT AAATTTGAAA CCAAAATAGA TTCATTTCAT
    GTTACTGGCT TACCAGATAA CTCAACAAAA CCCCGCCTCC TGTCTTCATT AGATGATGCT
    GCATCACTCT TGAAAATTAT GTTTGAGATA AATCCTTTAG ATGAAACTGT TGCTCAGAGG
    TGTATCATAG AAGCTGAACC TTTAGAAATG TTATATGATG CAAGAACAGT GAATAGTATA
    GTAGAATTCT TCAGACCTCC AAAAGAGGTA AATCTAGCAC AGCTGACTTC AGTAACTTTG
    ACAAAACTTG AAGAATTTCG TGATAAGACA GCAACAGGTC TGGTATATAT TATTGAAACA
    CAGAAAGTTC TTGATCTCAA AATTAACTTG AATGCTTCAT ATATCATTAT CCCACAAGAT
    GGTATCTTTA GCCCTACGTC AAATCTCCTT CTTTTGGATC TTGGACATCT AAAGGTGACA
    AGTAAAATTC GTTCAGAATT ACCAGATTTA AAACAAGGTG GAGCCAATCG TGAAGAGATA
    ATGCATAGAG CTTATGATTC CTTTGATATT CAACTTACAA GCATACAGCT GCTTTTCAGC
    AGAGTTGGTG ATAATTGGAA AGAAGCACGA AAGCTCAATG TGTCTGCACA GCATATCTTG
    GTACCTATGC ACTTCAATGT GGAGCTCTCT AAGGCCATGG TTTTCATGGA TATAAGTATG
    CCGAAATTCA AGATTTATGG AAACTTACCA TTAATTTCTT TACGAATCTC AGATAAAAAA
    CTGCAAGGGA TTTTGGAACT GATTGAAAGC ATTCCAAAAC CCTCACCTGC AACTGAAGTA
    AATGTGCCTG TCAAATCATC TCAGGTTCAA AGATCCACTT CTTTGGGAAC ATCACAGATG
    TCACAGAAGA AATTTCCTCT TTTGGAAATT CCATGTGTTG AAGATGATTC AGATGAGGAG
    TTTTTTGATG CACCATGTAG TCCGTTGGAA GAGCCTCTTC AATCACCATC TAGAATTAAA
    AGTCCTCAAC AGAAAAATCT TCAGAAACCA GATTGTTTTA AAAATATGAT TGAGTATAAA
    ATAAGATTTG AAGTGCCAGA GGTTTTGATT CAGTTCTGTC ACCTTGTTGG AGACTGTGAA
    CTACCCGTGG TAGAAATTAA TGTCTTAGGA TTGGGCACAG ATATTGAGCT TAGAACATTT
    GATTTGAAAG CAAATGCCTT TTTGAAAGAG GTCTGCTTAA AATGTCCAGA TTATTTTGAT
    GAAAACAAGA AACCAGTTTA TTTGATTACA ACACTGGATA ACACAATGGA AGATCTCTTA
    ACACTGGAAT ATGTGAAGGC TGAAAATAAT TTACCTGCAA CAATATTGAA GAATGCTTAT
    AACAGTGTTG TGCAACTGAT AAAGGTGAAT TTTTCCTCTT TGGATATTCA TTTACATACT
    GAAGCACTTT TGAATACAAT AAATTATTTT AATAATATCC TTCCATATTT GGAGGAAAAA
    CCAGCCCCAG TGCATGTTGT GGAGACTGAA GACAAAGAGG AGGTTGTTAA AAAAATACCT
    TTAAAATCAT CCAAGAATGA AGATACTATT ACCTTGCAGA TTTTAGCAGA ATTATCATGT
    TTACAGATCT TTATTCAAGA TCAGAAACGT AACATTTCCG AAATTAAGAT AGAAGGGCTT
    GATTGTGAAA TCATTATGAG GCCATCAGTG ACTGAAACAA ATGCAAAGCT AAGGAATATA
    ATTGTCCTGG ATTCTGATAT AACAGCTGTA TACAAAAAGG CTGTTTATAT TACCGGAAAA
    GAAGTTTTCA GCTTCAAAAT GGTTTCTTAT ATGGGTGCAA CTGCTGGTTC TGCATACACA
    GATATGAATG TAGTTGATAT GCAAGTTAAT TTAACTGTTG GTTGCATTGA AGTAGTTCTT
    GTCAAAAAAT TTCTGTATTC TATATTGGCT TTTATAGATA ATTTTCAAGC AGGTAAACAA
    GCCTTAGCGG AGGCAACTGT TCAGGCAGCA GGAATGGCTG CTACTGGTGT AAAAGAACTG
    GCACAGAGGA GTTCTAGAAT GGCATTGGAT GTTCACATCA AAGCCCCTGT TGTGGTTATC
    CCACAGTCTC CAGTTTCTGA AAATATTTTT GTTGCTGATT TTGGACTAAT TACAATGAAA
    AATACATTCC ATGTAATAAC AAAGAGTCCG ACCAACCCCC CACCCATTAT TGATTTGATA
    ACGATAAAGC TAAGTGAAAT GCGACTATAC AGGACTCAAT ATATTAATGA TGCATACCAG
    GAAGTACTGG ATCTACTATT GCCATTAAAT CTTGAGGTGA TGGTTGAGCG AAATTTATCG
    TGGGAGTGGT TCCAGGAAGT TCCTTGTTTC AATCTAAATG CTCAGCTAAA GCCCATGGAG
    TTCATTCTTA GTCAAGAAGA TATAACGACC ATTTTTAAAA CATTATATGG CAACATATGG
    TATGAAGGTG ATAGTGCTTC ACATGAGGTA ACAAAGGATC AGTCCAGTGT TACTAGTGGT
    GCTACTACTG CGTCACACCA TGCGGCAGGA ACAACTGTAG TGACAGCTGC TGTGGTAGAA
    GTACATTCAC ATGCTTCTCA AGTTAAGACA ACACTAAATA TGAGTTTCAG AACCGATTAT
    CTCACCATGG TACTGTATAG TCCAGGTCCT AAACAGACTT CCTTTACAGA TATTCGTGAT
    CCTTCTCTGA AACTTGCTGA ATTTAAGTTG GAGAATATTA TAAGCACTTT AAAAATGTAT
    ACAGATGACT CAATATTTTC TTCCTTCTCA TTAAAAAACT GTATTTTAGA TGACAAAAGG
    CCTCATATCA AGAAGGCAAC TCCTAGAATG ATAGGACTGA CAGTTGGGTT TGACAAAAAG
    GACATGATGG ATGTAAGGTA TAGGACGATC CGAGATGGTT GGGTGGCTGA TATAGTCCTT
    CAAGAAATGT ATATTTGTGC AAGTGTGGAA TTTCTGCTGA CAGTTGCAAA TGTCTTTCTT
    GAGGCCTACA CTACAGGCAC TGCTGTAGAA ACCAATGTTC AAACATGGTC TGCCAAAGAA
    GAAGTACCTA AACAGCAATC AGAAAAGTGG GAAATGAACA TCACTATTAA AAATCCTGAA
    ATTGTGTTTG TCGCTGATAT GACAAGAAAT GATGCTCCTG CCTTAGTCAT TACAACACAG
    TGTGAAATAT ATTGGCTAGG TGACTTTGAA AATGATACAA TGACTGCTGC CATTAAAGAT
    CTCCAAGTGA GAGCATGCCC ATTTCTTCCA GTCAAGAGAA AAGGAAAAAT CACTACTGTT
    TTGCAACCCT GTGATTTGTT CTATCAAGCT ACTCAGATAA GTACAGATCC ACAAGTACTT
    GAATTATCAG TAAAATCCCT GACACTGAAG GTTTCACCAG TTATCATAAA TACTGTGATT
    ACCATAACTT CAGCACTTTA TACTGCTAAG GAAACCATCC CAAAAGAAAC TGCTTCTTCT
    ACAGCACATT TATGGGAAAA GAAGGATACA AAAGATTTAA ATATGTGGTT TCTTGAAGAA
    CCAAATGAAG CTGTAAAAAG AGCTCCCATG CCTGAATTGG TACCCAAAGG AGAGATAATA
    AAAATGAACA TTGATTCTAT TTTTCTGGTT CTTGAGGCTG GAATTGGTCA TAGAACAGTA
    CCTATGCTAT TGGCAAAATC ACGTTTCTCA GGGGAAGGCA AAAACTGGAG TTCCTTAATA
    AATCTCCACT GTCAGCTTGA GCTAGAAGTA CATTATTACA ATGAAATGTT TGGTGTGTGG
    GAGCCTTTGC TTGAACCCTT AGAAATTGAT CAGACTGAGG ATTTTAGACC ATGGAATCTT
    GGAATCAAGA TGAAAAAGAA AGCCAAAAAG GCCATTGTTG AGTCAGGTAC TGAAGAAGAA
    AATTACAGAG TACCAGAATA CAAAACTGTC ATCACTTTCT ATTCGAAAGA CCAGCTAAAC
    ATTACATTAT CTAAATGTGG CCTTATAATG TTAAATAATT TAGCTGAGGC ATTTACAGAA
    GCTGCCACTG GATCTTCAGC TACCTTCATA AGGGATCTGG CACCTTTTAT GATTTTAAAT
    TCTCTTGGAC TTAGTATTTC TGTTTCACCA AGTGAATCTT TTAGTGTACT CAACGTACCT
    ATGGCAAAAT CATATGTATT GAAAAATGAG GAGAGTTTAA GTATGGATTA TATGCGAACT
    AAAGACAATG ATCATTTCAA TGCAATGACC AGCCTAAGCA GCAAACACTT CTTCATTCTT
    CTTACCCCTG CTAACCACTC TACTGCTGAT AAAATCCCTT TAACAAAAGT GGGACGACGA
    CTGTACACTG TAAGACACAG AGAGTCTGGA GTTGAAAGAT CAATTGTTTG TCAGATTGAT
    GCAGTGGAAG GAAGTAAGAA GGTAACAGTT CGCTCTCCAG TGCAGATTAG AAATCATTTT
    TCAATACCAC TTTCTGTTTA TGGAGGAGAC ACGTTATTGG GAACGGCCTC ACCTGGAAGT
    GAATTTAACA TACCATTAGC ATCTTACCGA TCACTACTTT CTCTGAAGCC AGAAGATGCA
    GACTATCAGA TGTGTGAAGG AATTGACTTT GAAGAAATTA TAAAACAAGA TGGTACTTCT
    TTCAAGAAAA AATGTAGACC TATAAACCCT TCCAGGAAGC CATTTATCAT TAATATAGTC
    CCAGAAAAAG ATAATTTGAC GTATATGTCA GTGTATTCAG AAGACCGTTG GGACTTACCA
    TATATAATAC ATTTGTGGCC ACCTATTCTA CTCCGAAATC TTCTTCCTTA CAAAATTGCT
    TATTACATAG AGGGGATTGA ACATAGAGTT TTTACTCTCC ATGAAGGCCG TTCAGCCCAG
    CTTTGTACTG TACAGTTGGA TAAAGCCAGG CTACATTTAA AATTACTTGA CTATCTTAAT
    CATGATTGGA AAAGTGAATA TCATATAAAA CCTAATCAAC AAGATATTAG TTTCATCAGT
    TTTACTTGTG TTACAGAAAT AGAAAAGACT GATTTAGATA TTGCTGTCCA TGTGACTTAT
    AATACTGGCC AGACTATTAT GGCATTTCAT AGTCCTTATT GGATGGTTAA TAAAACTGGT
    CGAATGTTGC AGTATAAAGC AGATGGAATT CATCGAAAGC ATCCACCTAA TTATAAAAAG
    CCAGTTCTCT TTTCATTTCA GCCAAAGCAC TTTTTCAATA ACAATAAGGT TCAGCTTATG
    GTAACTGATA GTGAATTGTC AGATCAATTT TCAATTGATA CAGTTGGTAG TCATGGAGCG
    GTTAAATGTA AAGGCCCAAA AATGGAATAT CAAGTTGGTG TCACTATAGA CCTTAGCAGT
    TTTAACATTA CCAGAATTGT CACATTTACC CCCTTTTTTT TGATTAAAAA CAAAAGCAAA
    TATCATATAT CAGTGGCTGA AGAGGGAAAT GATAAATGGC TCGCTCTTGA TTTGGAGCAG
    TGTGTCCCCT TTTGGCCTGA GGATGCTTCT AATAAACTTC TTATTCGAGT TGAAAGAAGT
    GAAAGCCCTC CCCATAAGAT ACATTTTAAT AAGGAAGAAA GTTGTATTTT ACTGCGTCTA
    GACAATGAGC TTGGAGGTAT TATAGCAGAA GTTAATTTGG CTGAGCATTC TACAGTTATT
    ACATTTTCAG ATTATCGTGA TGGAGCCGCT ACATTTCTGT TAATAAATCA CACCAAGAAT
    GACATTGTTC AGTATAAGCA AAGTTCCCTC AGTGAAATAG AAGATTCCCT CCCTCCTGGA
    AAAGCAGTAT TTTATACATG GGCTGATCCA GTGGGCTCTA GAAAGCTGCA GTGGAAATGT
    GGAAAAAGCC ACGGTGAAGT AACACAAAAG GATGATATGA TGACACCTAT AAATTTGGGG
    AAAACGACCA TTTATTTAGT TTCATTTTTT GAAGGCTTAC AACGCATAAT TTTATTCACT
    GAAGATCCAA GAGTATTTAA AGTGACATAT GAAAGTGAGA AAGCAGAGTT AGCAGAGCAA
    GAAATTGTGT TGGCGTTACA AGATGTTGGG ATTTCTCTTG TCAACAATTA TACAAAGCAA
    GAAGTAGCCT ATATTGGCAT TACAAGTTCT GATGTGGTTT GGGAGACAAA GCCCAGGAAG
    AAGGCAAGAT GGAAGCCAAT GAGTGTAAAG CACACTGAGA AGTTAGAGAA GGAATTTAAG
    GAATATGTTG AATCTTCACC GTCAGAAGAT AAGGTTATTG AGTTGGACAC CAACATTCTG
    GTTCGCTTCA CACCTGGTGC TTCTAACATG AAAATTCTGC AGCCACATGT AATAGCTATA
    AGAAGAAATT ATCTTCCAGC ATTAAAAGTA GAATATAGCA CATCTGCACA TCAGTCATCG
    TTTAGAATTC AAATTTATAG AATACAGATC CAAAACCAAA TACATGGTGC TATATTTCCA
    TTCGTGTTTT ATCCCATTAA ACCGCCCAAG TCCGTCACCT TGGATTCAGC ACCGAAACCC
    TTTACTGATG TCAGTATTGT CATGAGATCT GCAGGACATT CGCAGATATC ACGTATTAAG
    TATTTCAAAG TGTTGATCCA AGAAATGGAT CTCAGGTTAG ATCTTGGATT TGTCTATGCT
    GTATCAGAAC TTATGACACC AGCTGAAGTC ACTGAAAAAA CAGAGGTTGA GCTTTTTCAT
    AAAGACATAG AAGCTTTCCA AGAAGAATAT AAAATAGTCT CATTAGTAGA TTCATCACAA
    GTCAGTCTCT ATGAATACTT TCATATATCT CCTATCAAGT TGCATTTAAG TGTTTCACTG
    AGTTCAGGTG GTGAAGAACC GAAAGATTCA GAACAGCATG AAGGACTGAT GGCAATTCGT
    TCTTTACATC TTTTGCTGAA GAGTATTGGT GCCACTCTCA CAGATGTACA AGATGTAGTT
    TTTAAGCTAG CATTTTTTGA ACTCAATTAT CAGTTCCATA CAACAGCTGA ACTACAGTCT
    GAAGTAATAA GACACTATTC AAAGCAGGCC ATTAAACAGA TGTACGTACT CATTCTTGGA
    CTTGATGTTT TGGGAAATCC CTTTGGCTTG ATAAGAGAAT TTTCTGAAGG TGTAGAAGCA
    TTTTTTTATG AACCTTACCA GGGAGCCATC CAGGGTCCTG AAGAGTTTGT GGAAGGAATG
    GCACTAGGAC TGAAAGCACT AGTTGGTGGA GCTGTTGGTG GACTAGCTGG TGCTGCCTCC
    AAAATCACCA GTGCTATGGC TAAAGGGGTG GCAGCTATGA CTATGGATGA AGACTACCAA
    CAGAAGAGAA GAGAAGCCAT GAATAAGCAA CCAGCTGGTC TTAGGGAAGG CATCACTCGT
    GGAGGAAAAG GCCTAGTTTC TGGTTTTGTA AGTGGCATAA CAGGAATTGT TACAAAACCA
    ATCAAAGGAG CTCAGAAAGA AGGAGCAACT GGTTTCTTTA AAGGTGTTGG GAAAGGTTTA
    GTTGGAGCAG TGACAAGGCC AACTGGAGGC ATCATAGACA TGGCTAGCAG TACATTTCAG
    GGAATAAAAA GAGCTACAGA GACCTCTGAT GAAGTGGAGA GTCTGCGACC CCCTAGATTC
    TTTAATGAAG ATGGAGTTAT CAGACCTTAC AGATTGAGGG ATGGTACTGG AAATCAAATG
    TTACAGGTCA TGGAAAACGG AAGATTTGCA AAATACAAAT ATTTTACCCA TGTCATGATC
    AATAAGACAG ACATGTTAAT GATTACCAGA AGTGGTGTGT TGTTTGTAAC AAAAGGAACA
    TTTGGACAGC TTACTTGTGA GTGGCAGTAT AGTTTTGATG AGTTTACCAA AGAGCCATTC
    ATTGTTCATG AGAGAAGACT ACGCATCGAA GCAAAGGAAC GGGTGAAGTC TGTATTCCAT
    GCCAAAGAGT TTGGAAAAAT AGTTAACTTC AGAACCCCGG AGGATGCGAG GTGGATCCTC
    AGAAAGCTAG AAGAAGCAAG AGAACCTTCC CCGAACCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.